Lsi05G014540 (gene) Bottle gourd (USVL1VR-Ls) | Cucurbit Genomics Database (CuGenDB)

Overview


Name	Lsi05G014540
Type	gene
Organism	Lagenaria siceraria (Bottle gourd (USVL1VR-Ls))
Description	Basic helix-loop-helix DNA-binding superfamily protein
Location	chr05 : 22392843 .. 22394423 (+)

Genome Viewer

Gene Expression

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: CDS

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGAATGAACATCTTAGGGTTTTGAGATCTTTGACCCCTTCTTTCTATATCAAAAGGGTTTGATTCCCTTCAATCTCTCTCTCTCTTTTAATTTCCTCTTTCTTCTTCTTAATTTTCATTTGATGGGGTTTCCTTTTTATTTTATTATAGGGAGATCAAGCCTCCATTATTGGGGGAGTCATAGAGTTCATCAAGGAGTTGCATCAAGTTTTACAATCGTTGGAGTCCAACAAACGGAGGAGGAAGAGTATAAGCCCTAGTCCTGGTCCGAGCCCGAGACCACAGTTGATCGCGGGATCCGATAATGGCCCGTTTGGATTTGAAAATGGAGTTGATGTCGTCGGAGCGTGTTGCAATTCATCGGTTGCGGACGTCGAAGCAAAGATTTCGGGTTCGAACGTGATTTTGAAGATCATTTCTCGACGGATTCCCGGCCAACTTTCGAAGATGATTGGTGTCTTGGAGAGGCTCTCCTTTGAGGTTCTTCATCTCAACATTAGTAGTATGGATGACACGGTTCTGTACTCTTTTGTTGTCAAGGTATATACATCAAAATAGGGCTAACTAGCCTTTCTTTATTAATTAGGGTTTCATCTTTGGTTGAGGATATTACAAAAAGCCCACTTGAAACCCTAATTAATCTTCTTTATTTCACCCATAACAACAAAATTATCTATTTCTAAATAGGAATTAATGAAATTATAGATATTTTTTATACAAGAGGGTTGGATCAGTGGTGAGTACTATTATCTAAAAATTGATTGAATTTTTTTTTGAAAGCTCTTCTACATACAGATGTAATTAACTACAAAAAGTATTGGTGAATAGTAATTAGCAATCTTTATATGATAATGAAAACAATTTAGAATTAATTAACAAAATTATATTTGTTCAATCAAAAGTGACACTAGAAACTACTATTATAGTCAATTTTGTTACCCTAATTAACTATAAATATACGTAATGTATCAATATATATGATTACCACACAAATGCATTAGAAAAAGAGGGAGAAAAATTCATATATGTATTCAACTATTTAAAAAATACTTTTGGATGCAATCATATTCAATTTTAATCTATCTTTCACTTCATTTTGGAATTGAATTCTCAGCAAGTTCTAAGCTTTGAACTTCTCAGTTAGGGTTTATGAGTTTCCATTGTTAGCTATGAATCTATGGATTTAAATGCATGCATGCATACGATATCTTTTGAAAAAATTGACGAACAAAACTAGTTAAAACATTATATTATCCAAGTAACTTAATATGATTTGGGATAATCGATCTTATTTTTCGTATTAATGTAATATTGTTCCACATAAAATTTTCTTTTAAACTCTATATTTATGGATGTGTGTGATCTCAGAGTTTGCTTGAATAGAAAATTGGAGCATTTGAATAAGTTGTTACATTCTAAATGATTTGAATTTTGTTAATCATTTTCCTTAATTTTGTGAGATTTAATAACTCTGGTCTAATTTGTTGGATGCAGATAGGGCTTGAATGTCAGCTAAGTTTGGAGGAATTAGCTTATGAAGTTCAACAGAGCTTCTGCTCTCAAGTGTATTTGTGCCAGTGA

mRNA sequence

ATGAATGAACATCTTAGGGTTTTGAGATCTTTGACCCCTTCTTTCTATATCAAAAGGGGAGATCAAGCCTCCATTATTGGGGGAGTCATAGAGTTCATCAAGGAGTTGCATCAAGTTTTACAATCGTTGGAGTCCAACAAACGGAGGAGGAAGAGTATAAGCCCTAGTCCTGGTCCGAGCCCGAGACCACAGTTGATCGCGGGATCCGATAATGGCCCGTTTGGATTTGAAAATGGAGTTGATGTCGTCGGAGCGTGTTGCAATTCATCGGTTGCGGACGTCGAAGCAAAGATTTCGGGTTCGAACGTGATTTTGAAGATCATTTCTCGACGGATTCCCGGCCAACTTTCGAAGATGATTGGTGTCTTGGAGAGGCTCTCCTTTGAGGTTCTTCATCTCAACATTAGTAGTATGGATGACACGGTTCTGTACTCTTTTGTTGTCAAGATAGGGCTTGAATGTCAGCTAAGTTTGGAGGAATTAGCTTATGAAGTTCAACAGAGCTTCTGCTCTCAAGTGTATTTGTGCCAGTGA

Coding sequence (CDS)

ATGAATGAACATCTTAGGGTTTTGAGATCTTTGACCCCTTCTTTCTATATCAAAAGGGGAGATCAAGCCTCCATTATTGGGGGAGTCATAGAGTTCATCAAGGAGTTGCATCAAGTTTTACAATCGTTGGAGTCCAACAAACGGAGGAGGAAGAGTATAAGCCCTAGTCCTGGTCCGAGCCCGAGACCACAGTTGATCGCGGGATCCGATAATGGCCCGTTTGGATTTGAAAATGGAGTTGATGTCGTCGGAGCGTGTTGCAATTCATCGGTTGCGGACGTCGAAGCAAAGATTTCGGGTTCGAACGTGATTTTGAAGATCATTTCTCGACGGATTCCCGGCCAACTTTCGAAGATGATTGGTGTCTTGGAGAGGCTCTCCTTTGAGGTTCTTCATCTCAACATTAGTAGTATGGATGACACGGTTCTGTACTCTTTTGTTGTCAAGATAGGGCTTGAATGTCAGCTAAGTTTGGAGGAATTAGCTTATGAAGTTCAACAGAGCTTCTGCTCTCAAGTGTATTTGTGCCAGTGA

Protein sequence

MNEHLRVLRSLTPSFYIKRGDQASIIGGVIEFIKELHQVLQSLESNKRRRKSISPSPGPSPRPQLIAGSDNGPFGFENGVDVVGACCNSSVADVEAKISGSNVILKIISRRIPGQLSKMIGVLERLSFEVLHLNISSMDDTVLYSFVVKIGLECQLSLEELAYEVQQSFCSQVYLCQ

Homology

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BLAST of Lsi05G014540 vs. Swiss-Prot
Match: MUTE_ARATH (Transcription factor MUTE OS=Arabidopsis thaliana GN=MUTE PE=2 SV=1)

HSP 1 Score: 200.3 bits (508), Expect = 1.8e-50
Identity = 117/181 (64.64%), Postives = 136/181 (75.14%), Query Frame = 1


Query: 1   MNEHLRVLRSLTPSFYIKRGDQASIIGGVIEFIKELHQVLQSLESNKRRRKSISPS---P 60
           MNEHL+ LRSLTP FYIKRGDQASIIGGVIEFIKEL Q++Q LES KRR+    PS    
Sbjct: 14  MNEHLKSLRSLTPCFYIKRGDQASIIGGVIEFIKELQQLVQVLESKKRRKTLNRPSFPYD 73

Query: 61  GPSPRPQLI-AGSDNGPFG-FENGVDV-----VGACCNSSVADVEAKISGSNVILKIISR 120
             +  P  + A +   PF   EN +       VGACCNS  A+VEAKISGSNV+L+++SR
Sbjct: 74  HQTIEPSSLGAATTRVPFSRIENVMTTSTFKEVGACCNSPHANVEAKISGSNVVLRVVSR 133

Query: 121 RIPGQLSKMIGVLERLSFEVLHLNISSMDDTVLYSFVVKIGLECQLSLEELAYEVQQSFC 172
           RI GQL K+I VLE+LSF+VLHLNISSM++TVLY FVVKIGLEC LSLEEL  EVQ+SF 
Sbjct: 134 RIVGQLVKIISVLEKLSFQVLHLNISSMEETVLYFFVVKIGLECHLSLEELTLEVQKSFV 193

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BLAST of Lsi05G014540 vs. Swiss-Prot
Match: FAMA_ARATH (Transcription factor FAMA OS=Arabidopsis thaliana GN=FAMA PE=1 SV=1)

HSP 1 Score: 139.4 bits (350), Expect = 3.7e-32
Identity = 87/185 (47.03%), Postives = 114/185 (61.62%), Query Frame = 1


Query: 1   MNEHLRVLRSLTPSFYIKRGDQASIIGGVIEFIKELHQVLQSLESNKRRR--------KS 60
           MNEHLRVLRSL P  Y++RGDQASIIGG IEF++EL Q+LQ LES KRRR         +
Sbjct: 210 MNEHLRVLRSLMPGSYVQRGDQASIIGGAIEFVRELEQLLQCLESQKRRRILGETGRDMT 269

Query: 61  ISPSPGPSP--------RPQLIAGSDNGPFGFENGVDVVGACCNSSVADVEAKISGSNVI 120
            + +   SP        +P +I G+     G   G+    A   S +ADVE K+ G + +
Sbjct: 270 TTTTSSSSPITTVANQAQPLIITGNVTELEG-GGGLREETAENKSCLADVEVKLLGFDAM 329

Query: 121 LKIISRRIPGQLSKMIGVLERLSFEVLHLNISSMDDTVLYSFVVKIGLECQLSLEELAYE 170
           +KI+SRR PGQL K I  LE L   +LH NI++M+ TVLYSF VKI  E + + E++A  
Sbjct: 330 IKILSRRRPGQLIKTIAALEDLHLSILHTNITTMEQTVLYSFNVKITSETRFTAEDIASS 389

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BLAST of Lsi05G014540 vs. Swiss-Prot
Match: BH070_ARATH (Transcription factor bHLH70 OS=Arabidopsis thaliana GN=BHLH70 PE=2 SV=1)

HSP 1 Score: 104.4 bits (259), Expect = 1.3e-21
Identity = 74/173 (42.77%), Postives = 100/173 (57.80%), Query Frame = 1


Query: 1   MNEHLRVLRSLTPSFYIKRGDQASIIGGVIEFIKELHQVLQSLESNKRRRKSISPSPGPS 60
           MN HL  LRS+ PS YI+RGDQASI+GG I+F+K L Q LQSLE+ KR ++S   +    
Sbjct: 206 MNVHLNSLRSIIPSSYIQRGDQASIVGGAIDFVKILEQQLQSLEAQKRSQQS-DDNKEQI 265

Query: 61  PRPQLIAGSDNGPFGFENGVDVVGACCNSSVADVEAKISGSNVILKIISRRIPGQLSKMI 120
           P    +    +      N  +       SS   +EA +  S+V LKI   R  GQL + I
Sbjct: 266 PEDNSLRNISSNKLRASNKEE------QSSKLKIEATVIESHVNLKIQCTRKQGQLLRSI 325

Query: 121 GVLERLSFEVLHLNISSMDDT-VLYSFVVKIGLECQL-SLEELAYEVQQSFCS 172
            +LE+L F VLHLNI+S  +T V YSF +K+  EC L S +E+   ++Q F S
Sbjct: 326 ILLEKLRFTVLHLNITSPTNTSVSYSFNLKMEDECNLGSADEITAAIRQIFDS 371

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BLAST of Lsi05G014540 vs. Swiss-Prot
Match: BH057_ARATH (Transcription factor bHLH57 OS=Arabidopsis thaliana GN=BHLH57 PE=2 SV=1)

HSP 1 Score: 103.2 bits (256), Expect = 3.0e-21
Identity = 73/190 (38.42%), Postives = 101/190 (53.16%), Query Frame = 1


Query: 1   MNEHLRVLRSLTPSFYIKRGDQASIIGGVIEFIKELHQVLQSLESNKRRRKSISPSPGPS 60
           MNEHL  LRSL P  +++RGDQASI+GG I+FIKEL Q+LQSLE+ KR+        G  
Sbjct: 128 MNEHLNSLRSLMPPSFLQRGDQASIVGGAIDFIKELEQLLQSLEAEKRK-------DGTD 187

Query: 61  PRPQLIAGSDNGPFGFENGVDVVGACCNSSVA--------------------DVEAKISG 120
             P+  + S +             AC NSS++                    +VEA +  
Sbjct: 188 ETPKTASCSSSSSL----------ACTNSSISSVSTTSENGFTARFGGGDTTEVEATVIQ 247

Query: 121 SNVILKIISRRIPGQLSKMIGVLERLSFEVLHLNISSMDDTVLYSFVVKIGLECQL-SLE 170
           ++V LK+  +R   Q+ K I  +E L   +LHL ISS  D V+YSF +K+   C+L S +
Sbjct: 248 NHVSLKVRCKRGKRQILKAIVSIEELKLAILHLTISSSFDFVIYSFNLKMEDGCKLGSAD 300

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BLAST of Lsi05G014540 vs. Swiss-Prot
Match: SPCH_ARATH (Transcription factor SPEECHLESS OS=Arabidopsis thaliana GN=SPCH PE=1 SV=1)

HSP 1 Score: 102.4 bits (254), Expect = 5.0e-21
Identity = 54/114 (47.37%), Postives = 79/114 (69.30%), Query Frame = 1


Query: 61  PRPQLIAGSDNGPFGFENG----VDVVGACCNSSVADVEAKISGSNVILKIISRRIPGQL 120
           P P   A S + P    N     ++ + A   S++ADVE K SG+NV+LK +S +IPGQ+
Sbjct: 251 PPPYSPASSSSSPSVSSNHESSVINELVANSKSALADVEVKFSGANVLLKTVSHKIPGQV 310

Query: 121 SKMIGVLERLSFEVLHLNISSMDDTVLYSFVVKIGLECQLSLEELAYEVQQSFC 171
            K+I  LE L+ E+L +NI+++D+T+L SF +KIG+ECQLS EELA ++QQ+FC
Sbjct: 311 MKIIAALEDLALEILQVNINTVDETMLNSFTIKIGIECQLSAEELAQQIQQTFC 364

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BLAST of Lsi05G014540 vs. TrEMBL
Match: A0A0A0L8U9_CUCSA (Uncharacterized protein OS=Cucumis sativus GN=Csa_3G150010 PE=4 SV=1)

HSP 1 Score: 319.3 bits (817), Expect = 2.9e-84
Identity = 167/178 (93.82%), Postives = 172/178 (96.63%), Query Frame = 1


Query: 1   MNEHLRVLRSLTPSFYIKRGDQASIIGGVIEFIKELHQVLQSLESNKRRRKSISPSPGPS 60
           MNEHLRVLRSLTPSFYIKRGDQASIIGGVIEFIKELHQVLQSLESNKRRRKSISPSPGPS
Sbjct: 14  MNEHLRVLRSLTPSFYIKRGDQASIIGGVIEFIKELHQVLQSLESNKRRRKSISPSPGPS 73

Query: 61  PRPQLIA-GSDNGPFGFENGVDVVGACCNSSVADVEAKISGSNVILKIISRRIPGQLSKM 120
           P+ QL+A GSDN PFGFENGVDV GACCNSSVADVEAKISGSNV+LKIISRRIPGQL KM
Sbjct: 74  PKAQLVALGSDNSPFGFENGVDV-GACCNSSVADVEAKISGSNVVLKIISRRIPGQLPKM 133

Query: 121 IGVLERLSFEVLHLNISSMDDTVLYSFVVKIGLECQLSLEELAYEVQQSFCSQVYLCQ 178
           IGV ERLSFEVLHLNISSMDDTVLYSFVVKIGLECQLSLEELA+EVQQSFCSQ+YLCQ
Sbjct: 134 IGVFERLSFEVLHLNISSMDDTVLYSFVVKIGLECQLSLEELAFEVQQSFCSQLYLCQ 190

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BLAST of Lsi05G014540 vs. TrEMBL
Match: A0A0R6HST9_VITVI (MUTE OS=Vitis vinifera GN=MUTE PE=2 SV=1)

HSP 1 Score: 262.3 bits (669), Expect = 4.2e-67
Identity = 140/171 (81.87%), Postives = 154/171 (90.06%), Query Frame = 1


Query: 1   MNEHLRVLRSLTPSFYIKRGDQASIIGGVIEFIKELHQVLQSLESNKRRRKSISPSPGPS 60
           MNEHL+VLRSLTP FYIKRGDQASIIGGVIEFIKELHQVLQSLES K+RRKS+SPSPGPS
Sbjct: 14  MNEHLKVLRSLTPCFYIKRGDQASIIGGVIEFIKELHQVLQSLES-KKRRKSLSPSPGPS 73

Query: 61  PRPQLIAGSDNGPFGFENGVDVVGACCNSSVADVEAKISGSNVILKIISRRIPGQLSKMI 120
           PRP  +    + PFG EN  ++ GACCNSSVADVEAKISGSNVIL+IISRRIPGQ+ K+I
Sbjct: 74  PRPLQLTSQPDTPFGLENFKEL-GACCNSSVADVEAKISGSNVILRIISRRIPGQIVKII 133

Query: 121 GVLERLSFEVLHLNISSMDDTVLYSFVVKIGLECQLSLEELAYEVQQSFCS 172
            VLE+LSFEVLHLNISSM++TVLYSFVVKIGLECQLS+EELA EVQQSF S
Sbjct: 134 NVLEKLSFEVLHLNISSMEETVLYSFVVKIGLECQLSVEELALEVQQSFRS 182

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BLAST of Lsi05G014540 vs. TrEMBL
Match: A0A0R6HY81_VITRI (MUTE OS=Vitis riparia GN=MUTE PE=2 SV=1)

HSP 1 Score: 260.4 bits (664), Expect = 1.6e-66
Identity = 138/171 (80.70%), Postives = 153/171 (89.47%), Query Frame = 1


Query: 1   MNEHLRVLRSLTPSFYIKRGDQASIIGGVIEFIKELHQVLQSLESNKRRRKSISPSPGPS 60
           MNEHL+VLRSLTP FYIKRGDQASIIGGVIEFIKELHQVLQSLES K+RRKS+SPSPGPS
Sbjct: 14  MNEHLKVLRSLTPCFYIKRGDQASIIGGVIEFIKELHQVLQSLES-KKRRKSLSPSPGPS 73

Query: 61  PRPQLIAGSDNGPFGFENGVDVVGACCNSSVADVEAKISGSNVILKIISRRIPGQLSKMI 120
           PRP  +    + PFG EN  ++ GACCNSSVADVEAKISGSNVIL+IISRRIPGQ+ K+I
Sbjct: 74  PRPLQLTSQPDTPFGLENFKEL-GACCNSSVADVEAKISGSNVILRIISRRIPGQIVKII 133

Query: 121 GVLERLSFEVLHLNISSMDDTVLYSFVVKIGLECQLSLEELAYEVQQSFCS 172
            VLE+ SFEVLHLNISSM++TVLYSFV+KIGLECQLS+EELA EVQQSF S
Sbjct: 134 NVLEKFSFEVLHLNISSMEETVLYSFVIKIGLECQLSVEELALEVQQSFRS 182

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BLAST of Lsi05G014540 vs. TrEMBL
Match: A0A061EL05_THECC (Basic helix-loop-helix DNA-binding superfamily protein isoform 1 OS=Theobroma cacao GN=TCM_020520 PE=4 SV=1)

HSP 1 Score: 259.6 bits (662), Expect = 2.7e-66
Identity = 139/173 (80.35%), Postives = 155/173 (89.60%), Query Frame = 1


Query: 1   MNEHLRVLRSLTPSFYIKRGDQASIIGGVIEFIKELHQVLQSLESNKRRRKSISPSPGPS 60
           MNEHL+VLRSLTP FYIKRGDQASIIGGVIEFIKELHQVLQ+LES K+RRKS+SPSPGPS
Sbjct: 14  MNEHLKVLRSLTPCFYIKRGDQASIIGGVIEFIKELHQVLQALES-KKRRKSLSPSPGPS 73

Query: 61  PRP-QLIAGSDNGPFGFENGVDVVGACCNSSVADVEAKISGSNVILKIISRRIPGQLSKM 120
           PRP Q     D+ P GFE+ V  +GACCNSS+ADVEA+ISGSNVILKIIS+RIPGQ+ K+
Sbjct: 74  PRPLQQATQPDHSPIGFES-VGELGACCNSSLADVEARISGSNVILKIISKRIPGQILKI 133

Query: 121 IGVLERLSFEVLHLNISSMDDTVLYSFVVKIGLECQLSLEELAYEVQQSFCSQ 173
           I VLE+ SFEVLHLNISSM+DTVLYSFV+KIGLECQLSLEELA EVQQSF S+
Sbjct: 134 IAVLEKFSFEVLHLNISSMEDTVLYSFVIKIGLECQLSLEELAVEVQQSFFSE 184

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BLAST of Lsi05G014540 vs. TrEMBL
Match: A0A0R6HYB5_VITRI (MUTE OS=Vitis riparia GN=MUTE PE=4 SV=1)

HSP 1 Score: 257.7 bits (657), Expect = 1.0e-65
Identity = 137/170 (80.59%), Postives = 152/170 (89.41%), Query Frame = 1


Query: 2   NEHLRVLRSLTPSFYIKRGDQASIIGGVIEFIKELHQVLQSLESNKRRRKSISPSPGPSP 61
           NEHL+VLR LTP FYIKRGDQASIIGGVIEFIKELHQVLQSLES K+RRKS+SPSPGPSP
Sbjct: 15  NEHLKVLRFLTPCFYIKRGDQASIIGGVIEFIKELHQVLQSLES-KKRRKSLSPSPGPSP 74

Query: 62  RPQLIAGSDNGPFGFENGVDVVGACCNSSVADVEAKISGSNVILKIISRRIPGQLSKMIG 121
           RP  +    + PFG EN  ++ GACCNSSVADVEAKISGSNVIL+IISRRIPGQ+ K+I 
Sbjct: 75  RPLQLTSQPDTPFGLENFKEL-GACCNSSVADVEAKISGSNVILRIISRRIPGQIVKIIN 134

Query: 122 VLERLSFEVLHLNISSMDDTVLYSFVVKIGLECQLSLEELAYEVQQSFCS 172
           VLE+LSFEVLHLNISSM++TVLYSFV+KIGLECQLS+EELA EVQQSF S
Sbjct: 135 VLEKLSFEVLHLNISSMEETVLYSFVIKIGLECQLSVEELALEVQQSFRS 182

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BLAST of Lsi05G014540 vs. TAIR10
Match: AT3G06120.1 (AT3G06120.1 basic helix-loop-helix (bHLH) DNA-binding superfamily protein)

HSP 1 Score: 200.3 bits (508), Expect = 1.0e-51
Identity = 117/181 (64.64%), Postives = 136/181 (75.14%), Query Frame = 1


Query: 1   MNEHLRVLRSLTPSFYIKRGDQASIIGGVIEFIKELHQVLQSLESNKRRRKSISPS---P 60
           MNEHL+ LRSLTP FYIKRGDQASIIGGVIEFIKEL Q++Q LES KRR+    PS    
Sbjct: 14  MNEHLKSLRSLTPCFYIKRGDQASIIGGVIEFIKELQQLVQVLESKKRRKTLNRPSFPYD 73

Query: 61  GPSPRPQLI-AGSDNGPFG-FENGVDV-----VGACCNSSVADVEAKISGSNVILKIISR 120
             +  P  + A +   PF   EN +       VGACCNS  A+VEAKISGSNV+L+++SR
Sbjct: 74  HQTIEPSSLGAATTRVPFSRIENVMTTSTFKEVGACCNSPHANVEAKISGSNVVLRVVSR 133

Query: 121 RIPGQLSKMIGVLERLSFEVLHLNISSMDDTVLYSFVVKIGLECQLSLEELAYEVQQSFC 172
           RI GQL K+I VLE+LSF+VLHLNISSM++TVLY FVVKIGLEC LSLEEL  EVQ+SF 
Sbjct: 134 RIVGQLVKIISVLEKLSFQVLHLNISSMEETVLYFFVVKIGLECHLSLEELTLEVQKSFV 193

[Close]

BLAST of Lsi05G014540 vs. TAIR10
Match: AT3G24140.1 (AT3G24140.1 basic helix-loop-helix (bHLH) DNA-binding superfamily protein)

HSP 1 Score: 139.4 bits (350), Expect = 2.1e-33
Identity = 87/185 (47.03%), Postives = 114/185 (61.62%), Query Frame = 1


Query: 1   MNEHLRVLRSLTPSFYIKRGDQASIIGGVIEFIKELHQVLQSLESNKRRR--------KS 60
           MNEHLRVLRSL P  Y++RGDQASIIGG IEF++EL Q+LQ LES KRRR         +
Sbjct: 210 MNEHLRVLRSLMPGSYVQRGDQASIIGGAIEFVRELEQLLQCLESQKRRRILGETGRDMT 269

Query: 61  ISPSPGPSP--------RPQLIAGSDNGPFGFENGVDVVGACCNSSVADVEAKISGSNVI 120
            + +   SP        +P +I G+     G   G+    A   S +ADVE K+ G + +
Sbjct: 270 TTTTSSSSPITTVANQAQPLIITGNVTELEG-GGGLREETAENKSCLADVEVKLLGFDAM 329

Query: 121 LKIISRRIPGQLSKMIGVLERLSFEVLHLNISSMDDTVLYSFVVKIGLECQLSLEELAYE 170
           +KI+SRR PGQL K I  LE L   +LH NI++M+ TVLYSF VKI  E + + E++A  
Sbjct: 330 IKILSRRRPGQLIKTIAALEDLHLSILHTNITTMEQTVLYSFNVKITSETRFTAEDIASS 389

[Close]

BLAST of Lsi05G014540 vs. TAIR10
Match: AT2G46810.1 (AT2G46810.1 basic helix-loop-helix (bHLH) DNA-binding superfamily protein)

HSP 1 Score: 104.4 bits (259), Expect = 7.5e-23
Identity = 74/173 (42.77%), Postives = 100/173 (57.80%), Query Frame = 1


Query: 1   MNEHLRVLRSLTPSFYIKRGDQASIIGGVIEFIKELHQVLQSLESNKRRRKSISPSPGPS 60
           MN HL  LRS+ PS YI+RGDQASI+GG I+F+K L Q LQSLE+ KR ++S   +    
Sbjct: 206 MNVHLNSLRSIIPSSYIQRGDQASIVGGAIDFVKILEQQLQSLEAQKRSQQS-DDNKEQI 265

Query: 61  PRPQLIAGSDNGPFGFENGVDVVGACCNSSVADVEAKISGSNVILKIISRRIPGQLSKMI 120
           P    +    +      N  +       SS   +EA +  S+V LKI   R  GQL + I
Sbjct: 266 PEDNSLRNISSNKLRASNKEE------QSSKLKIEATVIESHVNLKIQCTRKQGQLLRSI 325

Query: 121 GVLERLSFEVLHLNISSMDDT-VLYSFVVKIGLECQL-SLEELAYEVQQSFCS 172
            +LE+L F VLHLNI+S  +T V YSF +K+  EC L S +E+   ++Q F S
Sbjct: 326 ILLEKLRFTVLHLNITSPTNTSVSYSFNLKMEDECNLGSADEITAAIRQIFDS 371

[Close]

BLAST of Lsi05G014540 vs. TAIR10
Match: AT4G01460.1 (AT4G01460.1 basic helix-loop-helix (bHLH) DNA-binding superfamily protein)

HSP 1 Score: 103.2 bits (256), Expect = 1.7e-22
Identity = 73/190 (38.42%), Postives = 101/190 (53.16%), Query Frame = 1


Query: 1   MNEHLRVLRSLTPSFYIKRGDQASIIGGVIEFIKELHQVLQSLESNKRRRKSISPSPGPS 60
           MNEHL  LRSL P  +++RGDQASI+GG I+FIKEL Q+LQSLE+ KR+        G  
Sbjct: 128 MNEHLNSLRSLMPPSFLQRGDQASIVGGAIDFIKELEQLLQSLEAEKRK-------DGTD 187

Query: 61  PRPQLIAGSDNGPFGFENGVDVVGACCNSSVA--------------------DVEAKISG 120
             P+  + S +             AC NSS++                    +VEA +  
Sbjct: 188 ETPKTASCSSSSSL----------ACTNSSISSVSTTSENGFTARFGGGDTTEVEATVIQ 247

Query: 121 SNVILKIISRRIPGQLSKMIGVLERLSFEVLHLNISSMDDTVLYSFVVKIGLECQL-SLE 170
           ++V LK+  +R   Q+ K I  +E L   +LHL ISS  D V+YSF +K+   C+L S +
Sbjct: 248 NHVSLKVRCKRGKRQILKAIVSIEELKLAILHLTISSSFDFVIYSFNLKMEDGCKLGSAD 300

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BLAST of Lsi05G014540 vs. TAIR10
Match: AT5G53210.1 (AT5G53210.1 basic helix-loop-helix (bHLH) DNA-binding superfamily protein)

HSP 1 Score: 102.4 bits (254), Expect = 2.8e-22
Identity = 54/114 (47.37%), Postives = 79/114 (69.30%), Query Frame = 1


Query: 61  PRPQLIAGSDNGPFGFENG----VDVVGACCNSSVADVEAKISGSNVILKIISRRIPGQL 120
           P P   A S + P    N     ++ + A   S++ADVE K SG+NV+LK +S +IPGQ+
Sbjct: 251 PPPYSPASSSSSPSVSSNHESSVINELVANSKSALADVEVKFSGANVLLKTVSHKIPGQV 310

Query: 121 SKMIGVLERLSFEVLHLNISSMDDTVLYSFVVKIGLECQLSLEELAYEVQQSFC 171
            K+I  LE L+ E+L +NI+++D+T+L SF +KIG+ECQLS EELA ++QQ+FC
Sbjct: 311 MKIIAALEDLALEILQVNINTVDETMLNSFTIKIGIECQLSAEELAQQIQQTFC 364

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BLAST of Lsi05G014540 vs. NCBI nr
Match: gi|659076510|ref|XP_008438719.1| (PREDICTED: transcription factor MUTE [Cucumis melo])

HSP 1 Score: 324.7 bits (831), Expect = 1.0e-85
Identity = 170/178 (95.51%), Postives = 173/178 (97.19%), Query Frame = 1


Query: 1   MNEHLRVLRSLTPSFYIKRGDQASIIGGVIEFIKELHQVLQSLESNKRRRKSISPSPGPS 60
           MNEHLRVLRSLTPSFYIKRGDQASIIGGVIEFIKELHQVLQSLESNKRRRKSISPSPGPS
Sbjct: 14  MNEHLRVLRSLTPSFYIKRGDQASIIGGVIEFIKELHQVLQSLESNKRRRKSISPSPGPS 73

Query: 61  PRPQLIA-GSDNGPFGFENGVDVVGACCNSSVADVEAKISGSNVILKIISRRIPGQLSKM 120
           PRPQL+A GSD  PFGFENGVDV GACCNSSVADVEAKISGSNV+LKIISRRIPGQLSKM
Sbjct: 74  PRPQLVALGSDKSPFGFENGVDV-GACCNSSVADVEAKISGSNVVLKIISRRIPGQLSKM 133

Query: 121 IGVLERLSFEVLHLNISSMDDTVLYSFVVKIGLECQLSLEELAYEVQQSFCSQVYLCQ 178
           IGV ERLSFEVLHLNISSMDDTVLYSFVVKIGLECQLSLEELAYEVQQSFCSQ+YLCQ
Sbjct: 134 IGVFERLSFEVLHLNISSMDDTVLYSFVVKIGLECQLSLEELAYEVQQSFCSQLYLCQ 190

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BLAST of Lsi05G014540 vs. NCBI nr
Match: gi|778678580|ref|XP_011650991.1| (PREDICTED: transcription factor MUTE [Cucumis sativus])

HSP 1 Score: 319.3 bits (817), Expect = 4.2e-84
Identity = 167/178 (93.82%), Postives = 172/178 (96.63%), Query Frame = 1


Query: 1   MNEHLRVLRSLTPSFYIKRGDQASIIGGVIEFIKELHQVLQSLESNKRRRKSISPSPGPS 60
           MNEHLRVLRSLTPSFYIKRGDQASIIGGVIEFIKELHQVLQSLESNKRRRKSISPSPGPS
Sbjct: 14  MNEHLRVLRSLTPSFYIKRGDQASIIGGVIEFIKELHQVLQSLESNKRRRKSISPSPGPS 73

Query: 61  PRPQLIA-GSDNGPFGFENGVDVVGACCNSSVADVEAKISGSNVILKIISRRIPGQLSKM 120
           P+ QL+A GSDN PFGFENGVDV GACCNSSVADVEAKISGSNV+LKIISRRIPGQL KM
Sbjct: 74  PKAQLVALGSDNSPFGFENGVDV-GACCNSSVADVEAKISGSNVVLKIISRRIPGQLPKM 133

Query: 121 IGVLERLSFEVLHLNISSMDDTVLYSFVVKIGLECQLSLEELAYEVQQSFCSQVYLCQ 178
           IGV ERLSFEVLHLNISSMDDTVLYSFVVKIGLECQLSLEELA+EVQQSFCSQ+YLCQ
Sbjct: 134 IGVFERLSFEVLHLNISSMDDTVLYSFVVKIGLECQLSLEELAFEVQQSFCSQLYLCQ 190

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BLAST of Lsi05G014540 vs. NCBI nr
Match: gi|794510301|gb|AKA58674.1| (MUTE [Vitis vinifera])

HSP 1 Score: 262.3 bits (669), Expect = 6.1e-67
Identity = 140/171 (81.87%), Postives = 154/171 (90.06%), Query Frame = 1


Query: 1   MNEHLRVLRSLTPSFYIKRGDQASIIGGVIEFIKELHQVLQSLESNKRRRKSISPSPGPS 60
           MNEHL+VLRSLTP FYIKRGDQASIIGGVIEFIKELHQVLQSLES K+RRKS+SPSPGPS
Sbjct: 14  MNEHLKVLRSLTPCFYIKRGDQASIIGGVIEFIKELHQVLQSLES-KKRRKSLSPSPGPS 73

Query: 61  PRPQLIAGSDNGPFGFENGVDVVGACCNSSVADVEAKISGSNVILKIISRRIPGQLSKMI 120
           PRP  +    + PFG EN  ++ GACCNSSVADVEAKISGSNVIL+IISRRIPGQ+ K+I
Sbjct: 74  PRPLQLTSQPDTPFGLENFKEL-GACCNSSVADVEAKISGSNVILRIISRRIPGQIVKII 133

Query: 121 GVLERLSFEVLHLNISSMDDTVLYSFVVKIGLECQLSLEELAYEVQQSFCS 172
            VLE+LSFEVLHLNISSM++TVLYSFVVKIGLECQLS+EELA EVQQSF S
Sbjct: 134 NVLEKLSFEVLHLNISSMEETVLYSFVVKIGLECQLSVEELALEVQQSFRS 182

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BLAST of Lsi05G014540 vs. NCBI nr
Match: gi|794510446|gb|AKA58680.1| (MUTE [Vitis riparia])

HSP 1 Score: 260.4 bits (664), Expect = 2.3e-66
Identity = 138/171 (80.70%), Postives = 153/171 (89.47%), Query Frame = 1


Query: 1   MNEHLRVLRSLTPSFYIKRGDQASIIGGVIEFIKELHQVLQSLESNKRRRKSISPSPGPS 60
           MNEHL+VLRSLTP FYIKRGDQASIIGGVIEFIKELHQVLQSLES K+RRKS+SPSPGPS
Sbjct: 14  MNEHLKVLRSLTPCFYIKRGDQASIIGGVIEFIKELHQVLQSLES-KKRRKSLSPSPGPS 73

Query: 61  PRPQLIAGSDNGPFGFENGVDVVGACCNSSVADVEAKISGSNVILKIISRRIPGQLSKMI 120
           PRP  +    + PFG EN  ++ GACCNSSVADVEAKISGSNVIL+IISRRIPGQ+ K+I
Sbjct: 74  PRPLQLTSQPDTPFGLENFKEL-GACCNSSVADVEAKISGSNVILRIISRRIPGQIVKII 133

Query: 121 GVLERLSFEVLHLNISSMDDTVLYSFVVKIGLECQLSLEELAYEVQQSFCS 172
            VLE+ SFEVLHLNISSM++TVLYSFV+KIGLECQLS+EELA EVQQSF S
Sbjct: 134 NVLEKFSFEVLHLNISSMEETVLYSFVIKIGLECQLSVEELALEVQQSFRS 182

[Close]

BLAST of Lsi05G014540 vs. NCBI nr
Match: gi|590657620|ref|XP_007034615.1| (Basic helix-loop-helix DNA-binding superfamily protein isoform 1 [Theobroma cacao])

HSP 1 Score: 259.6 bits (662), Expect = 3.9e-66
Identity = 139/173 (80.35%), Postives = 155/173 (89.60%), Query Frame = 1


Query: 1   MNEHLRVLRSLTPSFYIKRGDQASIIGGVIEFIKELHQVLQSLESNKRRRKSISPSPGPS 60
           MNEHL+VLRSLTP FYIKRGDQASIIGGVIEFIKELHQVLQ+LES K+RRKS+SPSPGPS
Sbjct: 14  MNEHLKVLRSLTPCFYIKRGDQASIIGGVIEFIKELHQVLQALES-KKRRKSLSPSPGPS 73

Query: 61  PRP-QLIAGSDNGPFGFENGVDVVGACCNSSVADVEAKISGSNVILKIISRRIPGQLSKM 120
           PRP Q     D+ P GFE+ V  +GACCNSS+ADVEA+ISGSNVILKIIS+RIPGQ+ K+
Sbjct: 74  PRPLQQATQPDHSPIGFES-VGELGACCNSSLADVEARISGSNVILKIISKRIPGQILKI 133

Query: 121 IGVLERLSFEVLHLNISSMDDTVLYSFVVKIGLECQLSLEELAYEVQQSFCSQ 173
           I VLE+ SFEVLHLNISSM+DTVLYSFV+KIGLECQLSLEELA EVQQSF S+
Sbjct: 134 IAVLEKFSFEVLHLNISSMEDTVLYSFVIKIGLECQLSLEELAVEVQQSFFSE 184

The following BLAST results are available for this feature:

BLAST of Lsi05G014540 vs. Swiss-Prot


Match Name	E-value	Identity	Description
MUTE_ARATH	1.8e-50	64.64	Transcription factor MUTE OS=Arabidopsis thaliana GN=MUTE PE=2 SV=1	[more]
FAMA_ARATH	3.7e-32	47.03	Transcription factor FAMA OS=Arabidopsis thaliana GN=FAMA PE=1 SV=1	[more]
BH070_ARATH	1.3e-21	42.77	Transcription factor bHLH70 OS=Arabidopsis thaliana GN=BHLH70 PE=2 SV=1	[more]
BH057_ARATH	3.0e-21	38.42	Transcription factor bHLH57 OS=Arabidopsis thaliana GN=BHLH57 PE=2 SV=1	[more]
SPCH_ARATH	5.0e-21	47.37	Transcription factor SPEECHLESS OS=Arabidopsis thaliana GN=SPCH PE=1 SV=1	[more]

BLAST of Lsi05G014540 vs. TrEMBL


Match Name	E-value	Identity	Description
A0A0A0L8U9_CUCSA	2.9e-84	93.82	Uncharacterized protein OS=Cucumis sativus GN=Csa_3G150010 PE=4 SV=1	[more]
A0A0R6HST9_VITVI	4.2e-67	81.87	MUTE OS=Vitis vinifera GN=MUTE PE=2 SV=1	[more]
A0A0R6HY81_VITRI	1.6e-66	80.70	MUTE OS=Vitis riparia GN=MUTE PE=2 SV=1	[more]
A0A061EL05_THECC	2.7e-66	80.35	Basic helix-loop-helix DNA-binding superfamily protein isoform 1 OS=Theobroma ca...	[more]
A0A0R6HYB5_VITRI	1.0e-65	80.59	MUTE OS=Vitis riparia GN=MUTE PE=4 SV=1	[more]

BLAST of Lsi05G014540 vs. TAIR10


Match Name	E-value	Identity	Description
AT3G06120.1	1.0e-51	64.64	basic helix-loop-helix (bHLH) DNA-binding superfamily protein	[more]
AT3G24140.1	2.1e-33	47.03	basic helix-loop-helix (bHLH) DNA-binding superfamily protein	[more]
AT2G46810.1	7.5e-23	42.77	basic helix-loop-helix (bHLH) DNA-binding superfamily protein	[more]
AT4G01460.1	1.7e-22	38.42	basic helix-loop-helix (bHLH) DNA-binding superfamily protein	[more]
AT5G53210.1	2.8e-22	47.37	basic helix-loop-helix (bHLH) DNA-binding superfamily protein	[more]

BLAST of Lsi05G014540 vs. NCBI nr


Match Name	E-value	Identity	Description
gi\|659076510\|ref\|XP_008438719.1\|	1.0e-85	95.51	PREDICTED: transcription factor MUTE [Cucumis melo]	[more]
gi\|778678580\|ref\|XP_011650991.1\|	4.2e-84	93.82	PREDICTED: transcription factor MUTE [Cucumis sativus]	[more]
gi\|794510301\|gb\|AKA58674.1\|	6.1e-67	81.87	MUTE [Vitis vinifera]	[more]
gi\|794510446\|gb\|AKA58680.1\|	2.3e-66	80.70	MUTE [Vitis riparia]	[more]
gi\|590657620\|ref\|XP_007034615.1\|	3.9e-66	80.35	Basic helix-loop-helix DNA-binding superfamily protein isoform 1 [Theobroma caca...	[more]

Annotated Terms

The following terms have been associated with this gene:

Vocabulary: **Molecular Function**
Term	Definition
GO:0046983	protein dimerization activity

Vocabulary: **INTERPRO**
Term	Definition
IPR011598	bHLH_dom

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0010374	stomatal complex development
biological_process	GO:0009913	epidermal cell differentiation
biological_process	GO:0006355	regulation of transcription, DNA-templated
biological_process	GO:0006470	protein dephosphorylation
biological_process	GO:0008150	biological_process
cellular_component	GO:0005634	nucleus
cellular_component	GO:0005667	transcription factor complex
cellular_component	GO:0005575	cellular_component
molecular_function	GO:0003677	DNA binding
molecular_function	GO:0046983	protein dimerization activity
molecular_function	GO:0003700	transcription factor activity, sequence-specific DNA binding
molecular_function	GO:0008138	protein tyrosine/serine/threonine phosphatase activity

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Lsi05G014540.1	Lsi05G014540.1	mRNA

InterPro

Analysis Name: InterPro Annotations of Lagenaria siceraria
Date Performed: 2017-09-18


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR011598	Myc-type, basic helix-loop-helix (bHLH) domain	GENE3D	G3DSA:4.10.280.10		coord: 1..48 score: 6.
IPR011598	Myc-type, basic helix-loop-helix (bHLH) domain	PROFILE	PS50888	BHLH	coord: 1..36 score: 10
IPR011598	Myc-type, basic helix-loop-helix (bHLH) domain	unknown	SSF47459	HLH, helix-loop-helix DNA-binding domain	coord: 1..49 score: 1.3
None	No IPR available	PANTHER	PTHR11514	MYC	coord: 1..171 score: 1.8
None	No IPR available	PANTHER	PTHR11514:SF49	TRANSCRIPTION FACTOR MUTE	coord: 1..171 score: 1.8

Synteny

The following gene(s) are orthologous to this gene:


Gene	Orthologue	Organism	Block
Lsi05G014540	Csa3G150010	Cucumber (Chinese Long) v2	culsiB199
Lsi05G014540	CSPI03G13480	Wild cucumber (PI 183967)	cpilsiB198
Lsi05G014540	Cucsa.254010	Cucumber (Gy14) v1	cgylsiB420
Lsi05G014540	CmaCh06G014320	Cucurbita maxima (Rimu)	cmalsiB757
Lsi05G014540	CmoCh06G014350	Cucurbita moschata (Rifu)	cmolsiB752
Lsi05G014540	Cla021661	Watermelon (97103) v1	lsiwmB362
Lsi05G014540	MELO3C006563	Melon (DHL92) v3.5.1	lsimeB369
Lsi05G014540	ClCG05G005410	Watermelon (Charleston Gray)	lsiwcgB333
Lsi05G014540	MELO3C006563.2	Melon (DHL92) v3.6.1	lsimedB385
Lsi05G014540	Cla97C05G086750	Watermelon (97103) v2	lsiwmbB330
Lsi05G014540	Bhi01G000628	Wax gourd	lsiwgoB432
Lsi05G014540	Cp4.1LG08g04260	Cucurbita pepo (Zucchini)	cpelsiB718
Lsi05G014540	CsGy3G013600	Cucumber (Gy14) v2	cgyblsiB184
Lsi05G014540	Carg17401	Silver-seed gourd	carlsiB552
Lsi05G014540	CsaV3_3G013690	Cucumber (Chinese Long) v3	cuclsiB218

The following gene(s) are paralogous to this gene:

None

The following block(s) are covering this gene:


Gene	Organism	Block
Lsi05G014540	Cucurbita maxima (Rimu)	cmalsiB250
Lsi05G014540	Cucurbita maxima (Rimu)	cmalsiB353
Lsi05G014540	Cucurbita maxima (Rimu)	cmalsiB834
Lsi05G014540	Cucurbita moschata (Rifu)	cmolsiB238
Lsi05G014540	Cucurbita moschata (Rifu)	cmolsiB342
Lsi05G014540	Cucurbita moschata (Rifu)	cmolsiB819
Lsi05G014540	Cucurbita pepo (Zucchini)	cpelsiB118
Lsi05G014540	Cucurbita pepo (Zucchini)	cpelsiB273
Lsi05G014540	Cucurbita pepo (Zucchini)	cpelsiB512
Lsi05G014540	Melon (DHL92) v3.5.1	lsimeB342
Lsi05G014540	Watermelon (Charleston Gray)	lsiwcgB345
Lsi05G014540	Watermelon (97103) v1	lsiwmB384
Lsi05G014540	Melon (DHL92) v3.6.1	lsimedB359
Lsi05G014540	Silver-seed gourd	carlsiB065
Lsi05G014540	Silver-seed gourd	carlsiB200
Lsi05G014540	Silver-seed gourd	carlsiB298
Lsi05G014540	Watermelon (97103) v2	lsiwmbB344
Lsi05G014540	Wax gourd	lsiwgoB422
Lsi05G014540	Bottle gourd (USVL1VR-Ls)	lsilsiB006