Lsi05G012130 (gene) Bottle gourd (USVL1VR-Ls) | Cucurbit Genomics Database (CuGenDB)

Overview


Name	Lsi05G012130
Type	gene
Organism	Lagenaria siceraria (Bottle gourd (USVL1VR-Ls))
Description	Divalent ion symporter
Location	chr05 : 19991345 .. 19995185 (+)

Genome Viewer

Gene Expression

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: five_prime_UTRCDSthree_prime_UTR

Hold the cursor over a type above to highlight its positions in the sequence below.

TGAACGGCTAATATGCTGAACACATCGATGCATAGTAATGTTCAAATTTATTTTTAGTTTAAAAATGAAAATAGGAGTTAAATTTTAATAATGGATTTGGGAATCACATTCCCAGATAACTTGAACAGCTCGACGAAATAGCTATGAAATTGATGACTGGCGGAAAAATTCTGTCTGTATGTAGGCATTATTTTATATAAAAGCCGGGAGCTCACCATGTGGACAGGAATGAAGTCCAACTTTTTGTTTGCTCTGAATTACTGTTAGAACCTCCATAGTATCCACTTTTGATTAGATTCCCATTCGTTTTCGAAGAGAAAGCAAGATTAGATCTCTTCTTTTTGGATCCAGATGCAAACAAATTTTTGTGTAAGTTCTTTAAGCTTCACTTGCAGGTTGCAAAAGTTGGTTCATCTTTATAATTTCTGATGATATATTTTCAATGATAGTGCATCAATTTGAGTCCATTTTGCCACACTTTCCTCTTTTTATGTAGGGTCCCTCTTAATCTTTTGTCTTTTAAACTTGAATTTGTTAACTTCCAATCTCGGACGTATTGTATTGGTTGTATTGGATTGGAGATTTCAAGTTCCATGTTTAAGCCATGAGAGCTTGGAGCTGGCCTTTCCCTGAAGTATGTGAAAAACCTGGAATTCTTGTTGGTCAAAGAATTTGGTAATTCCACTATTCTTCTTGGAAGATTTGGATTCTGACTTCCATGGCAATGACTACTTAGAAACTAATGTCACTGCCCAAATGCGATCCAGTTGGTAATTTGGTTTAGAATTGGCTGAAAAAGCAGAGTTTCAAGTACTGTTTGATACCATGTTCTTTTGTATAATTGTATGAGTCCTACTTCTAAAAGTTCTAGCTATTAGATTAATATTCATTCCTAATATATTGCCTGAACTTTTTGTAAATTAATGTTGCATGGCAAGTGGACTTCTTATCTTCAAAGTTAAAATATAGAGGAAGTGCGTACAAGTTGGCTCAATCAACATTTATCTCTTAGAGATTAGCTTTTAGAAAGTTAGATGTTTCATGTATAAATTCTTCACTGATGGTTTATGTTCAGAAATTTTGTCATCGTTTCAAGCTACATAGAAGGTTCCTTGTACTGCTTTTTTGTCTTTTGTTTATCCACCCTATCTTTGCGTTCCATCATTTTGGTTAATGGTTACAGTTTCTTTTTGTTAATATGACTCTGTTAAGATATTCAAACTAAATTTTCTTCTCAGGTTGGCTTGGGTGAGAAGTTGAATCAAGATACTCAGCTACTCTGGTGCTGAAAAGACATTCGAAATAAATTCTAGTCAATCAGGTTCAAGGCTGACTCAAGTCGAATCTGGTTGCAAACATGGCCATGGATCATACTGTAAAAGTCATTCTAGGCTCAATTGCCTTCGCAGTCTTCTGGTTATTAGCTGTTTTCCCTGCTATCCCATTTCTACCAATTGGGAGAACAGCAGGATCTCTCCTAGGGGCAATGCTAATGGTCGTATTTCGAGTCCTAACTCCAGATCAAGCATATGCAGCCATCGATCTCCCAATACTTGGTCTTCTGTTTGGGACAATGGTAGTTAGTGTTTATCTTGAAAGAGCTGATATGTTCAAGTATTTGGGTAAGGTGCTTTCGTGGAAGAGTAAAGGGGCAAAGGATTTAATTTGTCGAGTGTGTCTGATATCTGCAATTTCAAGTGCGTTTTTCACCAATGACACCTCGTGTGTGGTTTTGACTGAATTTGTTTTAAAAATTGCTAGGCAACATAACCTTCCACCCCGTCCCTTCCTTCTTGCCCTTGCATCAAGTGCAAATATTGGTTCTTCAGCAACACCTATTGGCAACCCCCAAAACTTGGTCATAGCTGTCCAGAGTAAAATTCCTTTTGGGCAATTTGTGGTTGGAATACTCCCAGCGATGCTGGTTGGTGTTGTTGTAAATGCTTTAATTATTCTTATCCTATATTGGAAATTGTTATCTGTCCAGAAGGATGAAGAAGATCCATCCCCAGAAGTTGTTGCAGACGAAGATGTGCTTTCTCATAGATTTTCACCAGCCAGATTGTCACACTCACAAATTCCATCCATTAACTCTGCAGAATGGGACTCTCGGTTGGATTTAATGAACACACAAAGTCCTCCGTGCTCAAATGCCAATGTTGAAACTCTAAGAAATTCAGTAAGTTCAAAAGATAATGAAATCCACAGGTCTCATAGTGCTATGACGGAGTCGGCAAGAATATCTGATGCTTCAAAAGAGTGGCTGCCCAATGCATCTCCTCAGAAAAGGGAAGAAGGTTTCTCTTCTAAGAGCCTCAACTCAATGGATAAACAGAAAGAACCAGTATCTTTGCAGCCCTCGGAAGGAAAGGAACATTGGAGCACCAAATGGAGAAAGATTGCATGGAAATGCTGTGTTTATCTTGTGACTATAGGAATGCTAGTTGCTTTATTGATGGGCTTGAATATGTCATGGACTGCAGTGACTGCTGCACTCGCTCTTGTAGTTCTTGATTTCAAGGATGCTCAGCCATGCCTAGAAAAGGTTTGAACCATTATTTCTTTGTCCATCTTTAATTGCTACAAATTATAATTTTTCGATGGAATTTAACGATACATTTTATGGTACTATTTCTACAGGTTTCCTATTCACTTTTAGTCTTCTTTTGTGGGATGTTTATGACGGTTGATGGATTTAACAAGACTGGATTACCAAGTGCCTTTTGGAATTTCATGGAGCCACATGCTCAGATTGATCGTGTTTCTGGGACTGTGGTTCTTGCTCTTGTCATACTGTACCTCTCAAACTTGGCTTCAAACGTGCCTACTGGTATTAAATATTTCTCCTCCCACTGTTCTTTGCTTTCCGAATTTTCAATGTTCTTTTCCATTTCTATCTTCTGAATGTTGATAGCCTTCATAGCATGAAGATTCCAATATAGCAAAGAAACCCTGAACTTCTTTACTTGGAGTTTTTTTGTAGATTTGGTATTTATCAAGAAATATCTGATTCTGAGTACAAGCACTACAACTGATGTGTATAAATAAGGTTGAAGTCATGAACATATTAACAAAATCACAAATTCATTTTCGTAGTATAAAGCAACAAACAGACTGAAAAAAGCACAGGTTGTCATAGATTGCTTAGCATTTCATGATTAGATGAGGATAGATAGGTACTAACTCAACAAGTTAATCTCTTCATTTGATTTAAGATTTTGAATGAAAAGTGATAACCACATAATTTTTCTTGATTGGTGAACATTATGTAATTCTCTGGTTCTATTGACCTGTTAATCGTCATGAAAATGCAATTGTATCTTATGCTTTTTCGTTGTCGTATTGTTAGTTCTTCTGCTTGGAGCACGGGTCGCCGCATCGGCTGCTGCAATATCCGCAGCTCAAGAAAAACGGGCATGGCTTCTTTTAGCTTGGATCAGTACTGTAGCTGGAAATCTCTCATTATTAGGCTCAGCTGCAAACTTGATAGTGTGCGAACAGGCTCGTCGCACTCCGCAGCTAAGCTACAATTTATCCTTCTGGAATCATCTTAAATTTGGAGTTCCCTCAACTCTTATTGTCACAGCCATTGGTTTATTTCTTATAAGATGATTTTCTTGCTTTTCCTTGCTTTCTTTTATTGTTTTTCCATAATAAACTTTTCATTTATGAAGGTTAGAATTGTAGATTTCGTTTGAAGTTAATCTATATATATATATATATATAAATATATGGAGAATGTTTCCTTGAAAAGTCTTGTACAAATTTAAGTATGAAACAGATGTTCGATATATGGTTGAGTTGAAAATGGAAATGAACTGATAAATAGCCATTTTTGTTC

mRNA sequence

TGAACGGCTAATATGCTGAACACATCGATGCATAGTAATGTTCAAATTTATTTTTAGTTTAAAAATGAAAATAGGAGTTAAATTTTAATAATGGATTTGGGAATCACATTCCCAGATAACTTGAACAGCTCGACGAAATAGCTATGAAATTGATGACTGGCGGAAAAATTCTGTCTGTATGTAGGCATTATTTTATATAAAAGCCGGGAGCTCACCATGTGGACAGGAATGAAGTCCAACTTTTTGTTTGCTCTGAATTACTGTTAGAACCTCCATAGTATCCACTTTTGATTAGATTCCCATTCGTTTTCGAAGAGAAAGCAAGATTAGATCTCTTCTTTTTGGATCCAGATGCAAACAAATTTTTGTGTTGGCTTGGGTGAGAAGTTGAATCAAGATACTCAGCTACTCTGGTGCTGAAAAGACATTCGAAATAAATTCTAGTCAATCAGGTTCAAGGCTGACTCAAGTCGAATCTGGTTGCAAACATGGCCATGGATCATACTGTAAAAGTCATTCTAGGCTCAATTGCCTTCGCAGTCTTCTGGTTATTAGCTGTTTTCCCTGCTATCCCATTTCTACCAATTGGGAGAACAGCAGGATCTCTCCTAGGGGCAATGCTAATGGTCGTATTTCGAGTCCTAACTCCAGATCAAGCATATGCAGCCATCGATCTCCCAATACTTGGTCTTCTGTTTGGGACAATGGTAGTTAGTGTTTATCTTGAAAGAGCTGATATGTTCAAGTATTTGGGTAAGGTGCTTTCGTGGAAGAGTAAAGGGGCAAAGGATTTAATTTGTCGAGTGTGTCTGATATCTGCAATTTCAAGTGCGTTTTTCACCAATGACACCTCGTGTGTGGTTTTGACTGAATTTGTTTTAAAAATTGCTAGGCAACATAACCTTCCACCCCGTCCCTTCCTTCTTGCCCTTGCATCAAGTGCAAATATTGGTTCTTCAGCAACACCTATTGGCAACCCCCAAAACTTGGTCATAGCTGTCCAGAGTAAAATTCCTTTTGGGCAATTTGTGGTTGGAATACTCCCAGCGATGCTGGTTGGTGTTGTTGTAAATGCTTTAATTATTCTTATCCTATATTGGAAATTGTTATCTGTCCAGAAGGATGAAGAAGATCCATCCCCAGAAGTTGTTGCAGACGAAGATGTGCTTTCTCATAGATTTTCACCAGCCAGATTGTCACACTCACAAATTCCATCCATTAACTCTGCAGAATGGGACTCTCGGTTGGATTTAATGAACACACAAAGTCCTCCGTGCTCAAATGCCAATGTTGAAACTCTAAGAAATTCAGTAAGTTCAAAAGATAATGAAATCCACAGGTCTCATAGTGCTATGACGGAGTCGGCAAGAATATCTGATGCTTCAAAAGAGTGGCTGCCCAATGCATCTCCTCAGAAAAGGGAAGAAGGTTTCTCTTCTAAGAGCCTCAACTCAATGGATAAACAGAAAGAACCAGTATCTTTGCAGCCCTCGGAAGGAAAGGAACATTGGAGCACCAAATGGAGAAAGATTGCATGGAAATGCTGTGTTTATCTTGTGACTATAGGAATGCTAGTTGCTTTATTGATGGGCTTGAATATGTCATGGACTGCAGTGACTGCTGCACTCGCTCTTGTAGTTCTTGATTTCAAGGATGCTCAGCCATGCCTAGAAAAGGTTTCCTATTCACTTTTAGTCTTCTTTTGTGGGATGTTTATGACGGTTGATGGATTTAACAAGACTGGATTACCAAGTGCCTTTTGGAATTTCATGGAGCCACATGCTCAGATTGATCGTGTTTCTGGGACTGTGGTTCTTGCTCTTGTCATACTGTACCTCTCAAACTTGGCTTCAAACGTGCCTACTGTTCTTCTGCTTGGAGCACGGGTCGCCGCATCGGCTGCTGCAATATCCGCAGCTCAAGAAAAACGGGCATGGCTTCTTTTAGCTTGGATCAGTACTGTAGCTGGAAATCTCTCATTATTAGGCTCAGCTGCAAACTTGATAGTGTGCGAACAGGCTCGTCGCACTCCGCAGCTAAGCTACAATTTATCCTTCTGGAATCATCTTAAATTTGGAGTTCCCTCAACTCTTATTGTCACAGCCATTGGTTTATTTCTTATAAGATGATTTTCTTGCTTTTCCTTGCTTTCTTTTATTGTTTTTCCATAATAAACTTTTCATTTATGAAGGTTAGAATTGTAGATTTCGTTTGAAGTTAATCTATATATATATATATATATAAATATATGGAGAATGTTTCCTTGAAAAGTCTTGTACAAATTTAAGTATGAAACAGATGTTCGATATATGGTTGAGTTGAAAATGGAAATGAACTGATAAATAGCCATTTTTGTTC

Coding sequence (CDS)

ATGGCCATGGATCATACTGTAAAAGTCATTCTAGGCTCAATTGCCTTCGCAGTCTTCTGGTTATTAGCTGTTTTCCCTGCTATCCCATTTCTACCAATTGGGAGAACAGCAGGATCTCTCCTAGGGGCAATGCTAATGGTCGTATTTCGAGTCCTAACTCCAGATCAAGCATATGCAGCCATCGATCTCCCAATACTTGGTCTTCTGTTTGGGACAATGGTAGTTAGTGTTTATCTTGAAAGAGCTGATATGTTCAAGTATTTGGGTAAGGTGCTTTCGTGGAAGAGTAAAGGGGCAAAGGATTTAATTTGTCGAGTGTGTCTGATATCTGCAATTTCAAGTGCGTTTTTCACCAATGACACCTCGTGTGTGGTTTTGACTGAATTTGTTTTAAAAATTGCTAGGCAACATAACCTTCCACCCCGTCCCTTCCTTCTTGCCCTTGCATCAAGTGCAAATATTGGTTCTTCAGCAACACCTATTGGCAACCCCCAAAACTTGGTCATAGCTGTCCAGAGTAAAATTCCTTTTGGGCAATTTGTGGTTGGAATACTCCCAGCGATGCTGGTTGGTGTTGTTGTAAATGCTTTAATTATTCTTATCCTATATTGGAAATTGTTATCTGTCCAGAAGGATGAAGAAGATCCATCCCCAGAAGTTGTTGCAGACGAAGATGTGCTTTCTCATAGATTTTCACCAGCCAGATTGTCACACTCACAAATTCCATCCATTAACTCTGCAGAATGGGACTCTCGGTTGGATTTAATGAACACACAAAGTCCTCCGTGCTCAAATGCCAATGTTGAAACTCTAAGAAATTCAGTAAGTTCAAAAGATAATGAAATCCACAGGTCTCATAGTGCTATGACGGAGTCGGCAAGAATATCTGATGCTTCAAAAGAGTGGCTGCCCAATGCATCTCCTCAGAAAAGGGAAGAAGGTTTCTCTTCTAAGAGCCTCAACTCAATGGATAAACAGAAAGAACCAGTATCTTTGCAGCCCTCGGAAGGAAAGGAACATTGGAGCACCAAATGGAGAAAGATTGCATGGAAATGCTGTGTTTATCTTGTGACTATAGGAATGCTAGTTGCTTTATTGATGGGCTTGAATATGTCATGGACTGCAGTGACTGCTGCACTCGCTCTTGTAGTTCTTGATTTCAAGGATGCTCAGCCATGCCTAGAAAAGGTTTCCTATTCACTTTTAGTCTTCTTTTGTGGGATGTTTATGACGGTTGATGGATTTAACAAGACTGGATTACCAAGTGCCTTTTGGAATTTCATGGAGCCACATGCTCAGATTGATCGTGTTTCTGGGACTGTGGTTCTTGCTCTTGTCATACTGTACCTCTCAAACTTGGCTTCAAACGTGCCTACTGTTCTTCTGCTTGGAGCACGGGTCGCCGCATCGGCTGCTGCAATATCCGCAGCTCAAGAAAAACGGGCATGGCTTCTTTTAGCTTGGATCAGTACTGTAGCTGGAAATCTCTCATTATTAGGCTCAGCTGCAAACTTGATAGTGTGCGAACAGGCTCGTCGCACTCCGCAGCTAAGCTACAATTTATCCTTCTGGAATCATCTTAAATTTGGAGTTCCCTCAACTCTTATTGTCACAGCCATTGGTTTATTTCTTATAAGATGA

Protein sequence

MAMDHTVKVILGSIAFAVFWLLAVFPAIPFLPIGRTAGSLLGAMLMVVFRVLTPDQAYAAIDLPILGLLFGTMVVSVYLERADMFKYLGKVLSWKSKGAKDLICRVCLISAISSAFFTNDTSCVVLTEFVLKIARQHNLPPRPFLLALASSANIGSSATPIGNPQNLVIAVQSKIPFGQFVVGILPAMLVGVVVNALIILILYWKLLSVQKDEEDPSPEVVADEDVLSHRFSPARLSHSQIPSINSAEWDSRLDLMNTQSPPCSNANVETLRNSVSSKDNEIHRSHSAMTESARISDASKEWLPNASPQKREEGFSSKSLNSMDKQKEPVSLQPSEGKEHWSTKWRKIAWKCCVYLVTIGMLVALLMGLNMSWTAVTAALALVVLDFKDAQPCLEKVSYSLLVFFCGMFMTVDGFNKTGLPSAFWNFMEPHAQIDRVSGTVVLALVILYLSNLASNVPTVLLLGARVAASAAAISAAQEKRAWLLLAWISTVAGNLSLLGSAANLIVCEQARRTPQLSYNLSFWNHLKFGVPSTLIVTAIGLFLIR

Homology

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BLAST of Lsi05G012130 vs. Swiss-Prot
Match: ARSB_DICDI (Putative transporter arsB OS=Dictyostelium discoideum GN=arsB PE=2 SV=1)

HSP 1 Score: 224.6 bits (571), Expect = 2.7e-57
Identity = 167/527 (31.69%), Postives = 281/527 (53.32%), Query Frame = 1


Query: 31  LPIGRTAGSLLGAMLMVVFRVLTPDQAYAAIDLPILGLLFGTMVVSVYLERADMFKYLGK 90
           LPIGR   S++GA LMV F ++ P +  + I+   + LL   M++S Y+E+A+++    K
Sbjct: 66  LPIGRAGSSIIGATLMVYFGIIQPKEIGSVINWDTIILLMSMMMLSNYMEQANIWGMASK 125

Query: 91  VLSWKSKGAKDLICRVCLISAISSAFFTNDTSCVVLTEFVLKIARQHNLPPRPFLLALAS 150
           +L WK K     + RVCLIS+I S+  TNDT CV LT  V+   +  NL   PFL+A+A+
Sbjct: 126 ILLWKCKSTSIFMVRVCLISSIMSSILTNDTVCVTLTPIVISACKSTNLTFFPFLMAIAT 185

Query: 151 SANIGSSATPIGNPQNLVIAVQSKIPFGQFVVGILPAMLVGVVVNALIILILYWKLLSVQ 210
           SANIGSSA P+GNPQN++IA    + F  F    + + ++GV +N +++L+ + K L   
Sbjct: 186 SANIGSSALPVGNPQNMIIATAGGLNFFNFFKVSIVSSILGVCLNTILLLLYFKKDLKNL 245

Query: 211 KDEEDPSPEVVAD--EDVLSHRFSPARLSHSQIPSINSAEWDSRLDLMNTQSPPCSNANV 270
               +   E V    E++ ++        H    + N ++ +  ++ +N +     + N 
Sbjct: 246 NSNFNQLIETVNPKVEEIDNNH-------HDDDGANNQSKNEKEMENINKEVEEEQHNND 305

Query: 271 ETLRNSVS-SKDNEIHRSHSAM-----TESARISDASKEWLPNASPQKREEGFSSKSLNS 330
           +   +  + +K+N  +  H+ +      +S  +SD S   + N   +K++E F     NS
Sbjct: 306 DDDDDGFNENKNNNNNGGHAILLVASSMDSIDLSDCS---IIN-KDKKKKENFIEIYFNS 365

Query: 331 MDKQKEPVSLQPSEGKEHWSTKWRKIAWKCCVYLVTIGMLVALLMGLNMSWTAVTAALAL 390
            +K  E +                K+ +K  V ++   +L+   +G++M +T +     L
Sbjct: 366 KEKSIETI------------VNIIKLIFKFRVAIILTLVLIGFFIGMHMGFTVLFGVSIL 425

Query: 391 VVLDFKDAQPCLEKVSYSLLVFFCGMFMTVDGFNKTGLPSAFWNFMEPHAQID----RVS 450
           ++ + KD    +  V + LL+FF G+F+ V+GF++     A W  +EP   ID     V 
Sbjct: 426 MICERKDITDIINSVDWELLLFFSGLFVLVEGFDRQFEKEA-WTILEPFVPIDSTHLNVL 485

Query: 451 GTVVLALVILYLSNLASNVPTVLLLGARVAASAAAISAAQEKRAWLLLAWISTVAGNLSL 510
              + +++IL L N+  NVP VL L  R+      + A      W+LLA++STVAGNL+L
Sbjct: 486 KIFIFSILILVLCNILGNVPLVLSLSPRL------LEALAPDFTWILLAFVSTVAGNLTL 545

Query: 511 LGSAANLIVCEQARRTPQLSYNLSFWNHLKFGVPSTLIVTAIGLFLI 546
           +GS ANLIV E+++      + + F  +LKFGVPST++V  IG+ ++
Sbjct: 546 VGSVANLIVAEKSKS----YHEIGFLEYLKFGVPSTILVILIGVPIV 558

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BLAST of Lsi05G012130 vs. Swiss-Prot
Match: P_PIG (P protein OS=Sus scrofa GN=Oca2 PE=2 SV=3)

HSP 1 Score: 89.0 bits (219), Expect = 1.8e-16
Identity = 135/569 (23.73%), Postives = 230/569 (40.42%), Query Frame = 1


Query: 1   MAMDHTVKVILGSIAFAVFWLLAVFPAIPFLPIGRTAGSLLGAM-----LMVVFRVLTPD 60
           MA  +    I   +  A   L  V+  I F  + RT  ++LG++     L V+    T  
Sbjct: 325 MAHQYLRASIEAQVTIAAVILAGVYVLIIFEIVHRTLAAMLGSLAALAALAVIGDRPTLT 384

Query: 61  QAYAAIDLPILGLLFGTMVVSVYLERADMFKYLGKVLSWKSKGAK-DLICRVCLISAISS 120
           Q    ID   L LLFG M++         F Y        S+G    +I  +CLI+A+ S
Sbjct: 385 QVVEWIDFETLALLFGMMILVAIFSETGFFDYCAVKAYQLSRGRVWAMIIMLCLIAAVLS 444

Query: 121 AFFTNDTSCVVLTEFVLKIARQHNLPPRPFLLALASSANIGSSATPIGNPQNLVIAVQSK 180
           AF  N T+ ++ T   +++    NL PR  L+A     NIG +AT IG+P N++I    +
Sbjct: 445 AFLDNVTTALLFTPVTIRLCEVLNLDPRQVLIAEVIFTNIGGAATAIGDPPNVIIVSNQE 504

Query: 181 I-PFGQFVVGILPAMLVGVVVNAL----IILILYWKLLSVQKDEEDPSPEVVADEDVLSH 240
           +   G    G    M  G+    L    ++ +LYW      K   +PS E+V     L H
Sbjct: 505 LRKMGLDFAGFTAHMFAGICFVLLFSFPLLRLLYWNRKLYNK---EPS-EIVE----LKH 564

Query: 241 RFSPARLSHSQIPSINSAEWDSRLDLMNTQSPPCSNANVETLRNSVSSKDNEIHRSHSAM 300
                RL+  +I   +  E   R  L+           V +L   ++ + +  HR     
Sbjct: 565 EIHVWRLTAQRISPASREETAVRGLLLE---------KVLSLERLLARRLHSFHR----- 624

Query: 301 TESARISDASKEWLPNASPQKREEGFSSKSLNSMDKQKEPVSLQPSEGKEHWSTKWRKIA 360
               +IS   K W  N    +++   S ++L +                           
Sbjct: 625 ----QISQEDKNWETNIQELQKKHRISDRTLLT--------------------------- 684

Query: 361 WKCCVYLVTIGMLVALL--------MGLNMSWTAVTAALALVVL-DFKDAQPCLEKVSYS 420
            KC   +  +G+++ +         + L++ W A+  A+ L++L D  D +  L +V ++
Sbjct: 685 -KC---VTVLGLVIFMFFLNSFVPGVHLDLGWIAILGAIWLLILADIHDFEIILHRVEWA 744

Query: 421 LLVFFCGMFMTVDG------FNKTGLPSAFWNFMEPHAQIDRVSGTVVLALVILYLSNLA 480
            L+FF  +F+ ++           G  +A    M P  Q    +  +V+  V    S+L 
Sbjct: 745 TLLFFAALFILMEALAHLHLIEYVGEQTALLIKMVPEDQ-RLAAAIIVVVWVSAIASSLI 804

Query: 481 SNVP-TVLLLGARVAASAAAISAAQEKRAWLLLAWISTVAGNLSLLGSAANLIVCEQARR 540
            N+P T  ++   +  S     +         LA  + + GN +L+G++AN +VC  A  
Sbjct: 805 DNIPFTATMIPVLLNLSRDPEISLPAPPLMYALALGACLGGNGTLIGASAN-VVC--AGI 832

Query: 541 TPQLSYNLSFWNHLKFGVPSTLIVTAIGL 543
             Q  Y  SF    + G P  ++   +G+
Sbjct: 865 AEQHGYGFSFMEFFRLGFPMMVVSCMVGM 832

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BLAST of Lsi05G012130 vs. Swiss-Prot
Match: P_HUMAN (P protein OS=Homo sapiens GN=OCA2 PE=1 SV=2)

HSP 1 Score: 80.9 bits (198), Expect = 4.8e-14
Identity = 128/549 (23.32%), Postives = 221/549 (40.26%), Query Frame = 1


Query: 14  IAFAVFWLLAVFPAIPFLPIGRTAGSLLGAMLMVVFRVLTPDQAYAA-----IDLPILGL 73
           +  A   L  V+  I F  + RT  ++LG++  +    +  D+         ID   L L
Sbjct: 331 VTIATAILAGVYALIIFEIVHRTLAAMLGSLAALAALAVIGDRPSLTHVVEWIDFETLAL 390

Query: 74  LFGTMVVSVYLERADMFKYLGKVLSWKSKGAK-DLICRVCLISAISSAFFTNDTSCVVLT 133
           LFG M++         F Y        S+G    +I  +CLI+A+ SAF  N T+ ++ T
Sbjct: 391 LFGMMILVAIFSETGFFDYCAVKAYRLSRGRVWAMIIMLCLIAAVLSAFLDNVTTMLLFT 450

Query: 134 EFVLKIARQHNLPPRPFLLALASSANIGSSATPIGNPQNLVIAVQSKI-PFGQFVVGILP 193
              +++    NL PR  L+A     NIG +AT IG+P N++I    ++   G    G   
Sbjct: 451 PVTIRLCEVLNLDPRQVLIAEVIFTNIGGAATAIGDPPNVIIVSNQELRKMGLDFAGFTA 510

Query: 194 AMLVGVVVNALI----ILILYWKLLSVQKDEEDPSPEVVADEDVLSHRFSPARLSHSQIP 253
            M +G+ +  L+    + +LYW      K   +PS E+V     L H     RL+  +I 
Sbjct: 511 HMFIGICLVLLVCFPLLRLLYWNRKLYNK---EPS-EIVE----LKHEIHVWRLTAQRIS 570

Query: 254 SINSAEWDSRLDLMNTQSPPCSNANVETLRNSVSSKDNEIHRSHSAMTESARISDASKEW 313
             +  E   R  L+           V  L + ++ + +  HR         +IS   K W
Sbjct: 571 PASREETAVRRLLL---------GKVLALEHLLARRLHTFHR---------QISQEDKNW 630

Query: 314 LPNASPQKREEGFSSKSLNSMDKQKEPVSLQPSEGKEHWSTKWRKIAWKCCVYLVTIGML 373
             N    +++   S   L                       K   +             +
Sbjct: 631 ETNIQELQKKHRISDGIL---------------------LAKCLTVLGFVIFMFFLNSFV 690

Query: 374 VALLMGLNMSWTAVTAALALVVL-DFKDAQPCLEKVSYSLLVFFCGMFMTVDG------F 433
             + +  ++ W A+  A+ L++L D  D +  L +V ++ L+FF  +F+ ++        
Sbjct: 691 PGIHL--DLGWIAILGAIWLLILADIHDFEIILHRVEWATLLFFAALFVLMEALAHLHLI 750

Query: 434 NKTGLPSAFWNFMEPHAQIDRVSGTVVLALVILYL-SNLASNVP-TVLLLGARVAASAAA 493
              G  +A    M P  Q  R+   +VL + +  L S+L  N+P T  ++   +  S   
Sbjct: 751 EYVGEQTALLIKMVPEEQ--RLIAAIVLVVWVSALASSLIDNIPFTATMIPVLLNLSHDP 810

Query: 494 ISAAQEKRAWLLLAWISTVAGNLSLLGSAANLIVCEQARRTPQLSYNLSFWNHLKFGVPS 543
                       LA+ + + GN +L+G++AN +VC  A    Q  Y  SF    + G P 
Sbjct: 811 EVGLPAPPLMYALAFGACLGGNGTLIGASAN-VVC--AGIAEQHGYGFSFMEFFRLGFPM 825

[Close]

BLAST of Lsi05G012130 vs. Swiss-Prot
Match: P_MOUSE (P protein OS=Mus musculus GN=Oca2 PE=1 SV=1)

HSP 1 Score: 79.3 bits (194), Expect = 1.4e-13
Identity = 132/561 (23.53%), Postives = 224/561 (39.93%), Query Frame = 1


Query: 14  IAFAVFWLLAVFPAIPFLPIGRTAGSLLGAMLMVVFRVLTPDQAYAA-----IDLPILGL 73
           +A AV  L  V+  I F  + RT  ++LGA+  +    +  D+         ID   L L
Sbjct: 326 VASAVAILAGVYTLIIFEIVHRTLAAMLGALAALAALAVVGDRPSLTHVVEWIDFETLAL 385

Query: 74  LFGTMVVSVYLERADMFKYLGKVLSWKSKGAK-DLICRVCLISAISSAFFTNDTSCVVLT 133
           LFG M++         F Y        S+G    +I  +CL++AI SAF  N T+ ++ T
Sbjct: 386 LFGMMILVAVFSETGFFDYCAVKAYQLSRGRVWAMIFMLCLMAAILSAFLDNVTTMLLFT 445

Query: 134 EFVLKIARQHNLPPRPFLLALASSANIGSSATPIGNPQNLVIAVQSKI-PFGQFVVGILP 193
              +++    NL PR  L+A     NIG +AT IG+P N++I    ++   G    G   
Sbjct: 446 PVTIRLCEVLNLDPRQVLIAEVIFTNIGGAATAIGDPPNVIIVSNQELRKMGLDFAGFTA 505

Query: 194 AMLVGV----VVNALIILILYWKLLSVQKDEEDPSPEVVADEDVLSHRFSPARLSHSQIP 253
            M +G+    +V+  ++ +LYW      K   +PS E+V     L H     RL+  +I 
Sbjct: 506 HMFLGICLVLLVSFPLLRLLYWNKKLYNK---EPS-EIVE----LKHEIHVWRLTAQRIS 565

Query: 254 SINSAEWDSRLDLMNTQSPPCSNANVETLRNSVSSKDNEIHRSHSAMTESARISDASKEW 313
             +  E   R  L+           V  L + ++ + +  HR         +IS   K W
Sbjct: 566 PASREETAVRGLLLE---------KVLALEHLLAQRLHTFHR---------QISQEDKNW 625

Query: 314 LPNASPQKREEGFSSKSLNSMDKQKEPVSLQPSEGKEHWSTKWRKIAWKCCV---YLVTI 373
             N    +R+   S +SL                              KC     +++++
Sbjct: 626 ETNIQELQRKHRISDRSL----------------------------LVKCLTVLGFVISM 685

Query: 374 GMLVALLMG----------LNMSWTAVTAALALVVLDFKDAQPCLEKVSYSLLVFFCGMF 433
             L + + G          L   W        L++ D  D +  L +V ++ L+FF  +F
Sbjct: 686 FFLNSFVPGIHLDLGWIAILGAIW-------LLILADIHDFEIILHRVEWATLLFFAALF 745

Query: 434 MTVDGFNK------TGLPSAFWNFMEPHAQIDRVSGTVVLALVILYL-SNLASNVP-TVL 493
           + ++           G  +A    M P  Q  R +  +VL + +  L S+L  N+P T  
Sbjct: 746 VLMEALTHLHLVEYVGEQTALLIKMVPEDQ--RFAAAIVLIVWVSALASSLIDNIPFTAT 805

Query: 494 LLGARVAASAAAISAAQEKRAWLLLAWISTVAGNLSLLGSAANLIVCEQARRTPQLSYNL 543
           ++   +  S     +         LA  + + GN +L+G++ N++    A +     Y  
Sbjct: 806 MIPVLLNLSQDPEISLPALPLMYALALGACLGGNGTLIGASTNVVCAGIAEKH---GYGF 820

[Close]

BLAST of Lsi05G012130 vs. Swiss-Prot
Match: Y2685_MYCTO (Uncharacterized transporter MT2759 OS=Mycobacterium tuberculosis (strain CDC 1551 / Oshkosh) GN=MT2759 PE=3 SV=1)

HSP 1 Score: 71.6 bits (174), Expect = 2.9e-11
Identity = 65/225 (28.89%), Postives = 114/225 (50.67%), Query Frame = 1


Query: 14  IAFAVFWLLAVFPAIPFLPIGRTAGSLLGAMLMVVFRVLTPDQAY----AAIDLPILGLL 73
           IA  VF  +A +  I    + +T  +L  A +MV   ++  D  +    A ID  ++ LL
Sbjct: 4   IAITVF--VAGYALIASDRVSKTRVALTCAAIMVGAGIVGSDDVFYSHEAGIDWDVIFLL 63

Query: 74  FGTMVVSVYLERADMFKYLGKVLSWKSKGAKDLICRVC----LISAISSAFFTNDTSCVV 133
            G M++   L    +F+Y   V  W  K A     R+     L++A+ SA   N T+ ++
Sbjct: 64  LGMMIIVSVLRHTGVFEY---VAIWAVKRANAAPLRIMILLVLVTALGSALLDNVTTVLL 123

Query: 134 LTEFVLKIARQHNLPPRPFLLALASSANIGSSATPIGNPQNLVIAVQSKIPFGQFVVGIL 193
           +    L +  +  +   PFL+A   ++N+G +AT +G+P N++IA ++ + F  F++ + 
Sbjct: 124 IAPVTLLVCDRLGVNSTPFLVAEVFASNVGGAATLVGDPPNIIIASRAGLTFNDFLIHMA 183

Query: 194 PAMLVGVVVNALIILILYWKLLSVQKDEEDPSPEVVADEDVLSHR 231
           PA+LV ++    +I +L W L SV  +     P+ VAD   L+ R
Sbjct: 184 PAVLVVMIA---LIGLLPWLLGSVTAE-----PDRVADVLSLNER 215

[Close]

BLAST of Lsi05G012130 vs. TrEMBL
Match: A0A0A0L6L7_CUCSA (Uncharacterized protein OS=Cucumis sativus GN=Csa_3G182780 PE=4 SV=1)

HSP 1 Score: 988.0 bits (2553), Expect = 4.5e-285
Identity = 518/547 (94.70%), Postives = 532/547 (97.26%), Query Frame = 1


Query: 1   MAMDHTVKVILGSIAFAVFWLLAVFPAIPFLPIGRTAGSLLGAMLMVVFRVLTPDQAYAA 60
           MAMDHTVKVILGSIAFAVFWLLAVFPAIPFLPIGRTAGS+LGAMLMVVFRVLTP+QAYAA
Sbjct: 1   MAMDHTVKVILGSIAFAVFWLLAVFPAIPFLPIGRTAGSILGAMLMVVFRVLTPEQAYAA 60

Query: 61  IDLPILGLLFGTMVVSVYLERADMFKYLGKVLSWKSKGAKDLICRVCLISAISSAFFTND 120
           IDLPILGLLFGTMVVSVYLERADMFKYLGKVLSWKSKGAKDLICRVCLISAISSAFFTND
Sbjct: 61  IDLPILGLLFGTMVVSVYLERADMFKYLGKVLSWKSKGAKDLICRVCLISAISSAFFTND 120

Query: 121 TSCVVLTEFVLKIARQHNLPPRPFLLALASSANIGSSATPIGNPQNLVIAVQSKIPFGQF 180
           TSCVVLTEFVLKIARQHNLPPRPFLLALASSANIGSSATPIGNPQNLVIAVQSKI FGQF
Sbjct: 121 TSCVVLTEFVLKIARQHNLPPRPFLLALASSANIGSSATPIGNPQNLVIAVQSKIHFGQF 180

Query: 181 VVGILPAMLVGVVVNALIILILYWKLLSVQKDEEDPSPEVVADEDVLSHRFSPARLSHSQ 240
           V+GILPAMLVGVVVNALIILI+YWKLLSVQKDEEDPSPEV+ADEDVLSHRFSPARLSHSQ
Sbjct: 181 VIGILPAMLVGVVVNALIILIMYWKLLSVQKDEEDPSPEVIADEDVLSHRFSPARLSHSQ 240

Query: 241 IPSINSAEWDSRLDLMNTQSPPCSNANVETLRNSVSSKDN-EIHRSHSAMTESARISDAS 300
           IPS+NSAEWDSRLDLMN QSPPCSN NVET+RNSVSSKDN EI RSHS MTE ARISDAS
Sbjct: 241 IPSLNSAEWDSRLDLMNAQSPPCSNTNVETIRNSVSSKDNEEIRRSHSTMTEPARISDAS 300

Query: 301 KEWLPNASPQKREEGFSSKSLNSMDKQKEPVSLQPSEGKEHWSTKWRKIAWKCCVYLVTI 360
           KEWLPNAS QKREE FSSKSLNSM+KQKEPV LQ SEGKEHWSTKWR+IAWK CVYLVT+
Sbjct: 301 KEWLPNASTQKREEDFSSKSLNSMEKQKEPVILQSSEGKEHWSTKWRRIAWKSCVYLVTV 360

Query: 361 GMLVALLMGLNMSWTAVTAALALVVLDFKDAQPCLEKVSYSLLVFFCGMFMTVDGFNKTG 420
           GMLVALLMGL+MSWTAVTAALALVVLDFKDAQPCLEKVSYSLLVFFCGMFMTVDGFNKTG
Sbjct: 361 GMLVALLMGLDMSWTAVTAALALVVLDFKDAQPCLEKVSYSLLVFFCGMFMTVDGFNKTG 420

Query: 421 LPSAFWNFMEPHAQIDRVSGTVVLALVILYLSNLASNVPTVLLLGARVAASAAAISAAQE 480
           LPSAFWNFMEPHAQIDRVSGTVVLALVILYLSNLASNVPTVLLLGARVAASAAAIS  +E
Sbjct: 421 LPSAFWNFMEPHAQIDRVSGTVVLALVILYLSNLASNVPTVLLLGARVAASAAAISPTEE 480

Query: 481 KRAWLLLAWISTVAGNLSLLGSAANLIVCEQARRTPQLSYNLSFWNHLKFGVPSTLIVTA 540
           KRAWLLLAWISTVAGNLSLLGSAANLIVCEQARRTPQLSYNLSFWNHLKFG+PSTLIVTA
Sbjct: 481 KRAWLLLAWISTVAGNLSLLGSAANLIVCEQARRTPQLSYNLSFWNHLKFGLPSTLIVTA 540

Query: 541 IGLFLIR 547
           IGL LI+
Sbjct: 541 IGLVLIK 547

[Close]

BLAST of Lsi05G012130 vs. TrEMBL
Match: F1SX52_CUCMO (Silicon efflux transporter CmLsi2-1 OS=Cucurbita moschata GN=CmLsi2-1 PE=2 SV=1)

HSP 1 Score: 951.8 bits (2459), Expect = 3.6e-274
Identity = 501/549 (91.26%), Postives = 522/549 (95.08%), Query Frame = 1


Query: 1   MAMDHTVKVILGSIAFAVFWLLAVFPAIPFLPIGRTAGSLLGAMLMVVFRVLTPDQAYAA 60
           MAMDHTVKVILGSIAF +FWLLAVFPAIPFLPIGRTAGSLLGAMLMV+FRVLTPDQAYAA
Sbjct: 1   MAMDHTVKVILGSIAFGIFWLLAVFPAIPFLPIGRTAGSLLGAMLMVLFRVLTPDQAYAA 60

Query: 61  IDLPILGLLFGTMVVSVYLERADMFKYLGKVLSWKSKGAKDLICRVCLISAISSAFFTND 120
           IDLPILGLLFGTMVVSVYLERADMFKYLGKVLSWKSKGAKDLICRVCLISAISSA FTND
Sbjct: 61  IDLPILGLLFGTMVVSVYLERADMFKYLGKVLSWKSKGAKDLICRVCLISAISSALFTND 120

Query: 121 TSCVVLTEFVLKIARQHNLPPRPFLLALASSANIGSSATPIGNPQNLVIAVQSKIPFGQF 180
           TSCVVLTEFVLKIA+QHNLPPRPFLLALASSANIGSSATPIGNPQNLVIAVQS IPFGQF
Sbjct: 121 TSCVVLTEFVLKIAKQHNLPPRPFLLALASSANIGSSATPIGNPQNLVIAVQSGIPFGQF 180

Query: 181 VVGILPAMLVGVVVNALIILILYWKLLSVQKDEEDPSPEVVADEDVLSHRFSPARLSHSQ 240
           V+GILPAMLVG+VVNALIILI YWKLLS+QKDEED  PE+VADEDV SHRFSPARLSHSQ
Sbjct: 181 VIGILPAMLVGIVVNALIILISYWKLLSIQKDEEDVPPEIVADEDVNSHRFSPARLSHSQ 240

Query: 241 IPSINSAEWDSRLDLMNTQSPPCSNANVETLRNSVSSKDNEIHRSHSAMTES-ARISDAS 300
           IPS+NS EWDSRLD M  QSPP SNANVETLR S+SSKDNEIH SH A+TE+ ARISDAS
Sbjct: 241 IPSLNSQEWDSRLDSMYAQSPPNSNANVETLRTSLSSKDNEIHWSHGAVTEAVARISDAS 300

Query: 301 KEWLPNAS--PQKREEGFSSKSLNSMDKQKEPVSLQPSEGKEHWSTKWRKIAWKCCVYLV 360
           KE +PNAS  PQKREEG SSKS NSMDKQKEP+ LQ SEGK+HWSTKWR++AWKCCVYLV
Sbjct: 301 KECVPNASPPPQKREEGLSSKSFNSMDKQKEPLPLQVSEGKQHWSTKWRRMAWKCCVYLV 360

Query: 361 TIGMLVALLMGLNMSWTAVTAALALVVLDFKDAQPCLEKVSYSLLVFFCGMFMTVDGFNK 420
           TIGMLVALLMGLNMSWTAVTAALALVVLDFKDAQPCLEKVSYSLLVFFCGMF+TVDGFNK
Sbjct: 361 TIGMLVALLMGLNMSWTAVTAALALVVLDFKDAQPCLEKVSYSLLVFFCGMFITVDGFNK 420

Query: 421 TGLPSAFWNFMEPHAQIDRVSGTVVLALVILYLSNLASNVPTVLLLGARVAASAAAISAA 480
           TGLP+ FWNFMEPHAQIDRVSGTVVLALVILYLSNLASNVPTVLLLGARVAASAAAIS A
Sbjct: 421 TGLPNTFWNFMEPHAQIDRVSGTVVLALVILYLSNLASNVPTVLLLGARVAASAAAISPA 480

Query: 481 QEKRAWLLLAWISTVAGNLSLLGSAANLIVCEQARRTPQLSYNLSFWNHLKFGVPSTLIV 540
           QEKRAWLLLAWISTVAGNLSLLGSAANLIVCEQARRTPQLSYNLSFWNHLKFG+PSTLI+
Sbjct: 481 QEKRAWLLLAWISTVAGNLSLLGSAANLIVCEQARRTPQLSYNLSFWNHLKFGLPSTLII 540

Query: 541 TAIGLFLIR 547
           T IGL LI+
Sbjct: 541 TGIGLVLIK 549

[Close]

BLAST of Lsi05G012130 vs. TrEMBL
Match: F1SX53_CUCMO (Silicon efflux transporter CmLsi2-2 OS=Cucurbita moschata GN=CmLsi2-2 PE=2 SV=1)

HSP 1 Score: 932.6 bits (2409), Expect = 2.2e-268
Identity = 501/546 (91.76%), Postives = 514/546 (94.14%), Query Frame = 1


Query: 1   MAMDHTVKVILGSIAFAVFWLLAVFPAIPFLPIGRTAGSLLGAMLMVVFRVLTPDQAYAA 60
           MAMDHTVKVILGSIAFA FWLLAVFPAIPFLP+GRTAGSLLGAMLMVVFRVLTPDQAYAA
Sbjct: 1   MAMDHTVKVILGSIAFATFWLLAVFPAIPFLPVGRTAGSLLGAMLMVVFRVLTPDQAYAA 60

Query: 61  IDLPILGLLFGTMVVSVYLERADMFKYLGKVLSWKSKGAKDLICRVCLISAISSAFFTND 120
           IDLPILGLLFGTMVVSVYLERADMFKYLGKVLSW+SKGAKDLICRVCLISAISSAFFTND
Sbjct: 61  IDLPILGLLFGTMVVSVYLERADMFKYLGKVLSWRSKGAKDLICRVCLISAISSAFFTND 120

Query: 121 TSCVVLTEFVLKIARQHNLPPRPFLLALASSANIGSSATPIGNPQNLVIAVQSKIPFGQF 180
           TSCVVLTEFVLKIARQHNLPPRPFLLALASSANIGSSATPIGNPQNLVIAVQSKIPFG+F
Sbjct: 121 TSCVVLTEFVLKIARQHNLPPRPFLLALASSANIGSSATPIGNPQNLVIAVQSKIPFGEF 180

Query: 181 VVGILPAMLVGVVVNALIILILYWKLLSVQKDEEDPSPEVVADEDVLSHRFSPARLSHSQ 240
           VVGILPAMLVGVVVNALIILILYWKLLSVQKDEEDPS E+VADE VLSHRFSPARLSH+Q
Sbjct: 181 VVGILPAMLVGVVVNALIILILYWKLLSVQKDEEDPSSEIVADEVVLSHRFSPARLSHTQ 240

Query: 241 IPSINSAEWDSRLDLMNTQSPPCSNANVETLRNSVSSKDNEIHRSHSAMTESARISDASK 300
           IPS+NS EW+SRLD M        NANVET R SVSSK+NEIHRSHSA+TESARISDAS 
Sbjct: 241 IPSLNSPEWESRLDSM--------NANVETQR-SVSSKENEIHRSHSALTESARISDASS 300

Query: 301 EWLPNASPQKREEGFSSKSLNSMDKQKEPVSLQPSEGKEHWSTKWRKIAWKCCVYLVTIG 360
                    KREEGFSSKSLNSMDKQKE VSL  SE KEHWSTKWR+IAWKCCVYLVTIG
Sbjct: 301 --------LKREEGFSSKSLNSMDKQKESVSLPSSEEKEHWSTKWRRIAWKCCVYLVTIG 360

Query: 361 MLVALLMGLNMSWTAVTAALALVVLDFKDAQPCLEKVSYSLLVFFCGMFMTVDGFNKTGL 420
           MLVALLMGLNMSWTAVTAALALVVLDFKDAQPCLEKVSYSLLVFFCGMFMTVDGFNKTGL
Sbjct: 361 MLVALLMGLNMSWTAVTAALALVVLDFKDAQPCLEKVSYSLLVFFCGMFMTVDGFNKTGL 420

Query: 421 PSAFWNFMEPHAQIDRVSGTVVLALVILYLSNLASNVPTVLLLGARVAASAAAISAAQEK 480
           PSAFW+FMEPHAQIDRVSGTVVLALVILYLSNLASNVPTVLLLGARVAASAAAIS  +EK
Sbjct: 421 PSAFWDFMEPHAQIDRVSGTVVLALVILYLSNLASNVPTVLLLGARVAASAAAISPMEEK 480

Query: 481 RAWLLLAWISTVAGNLSLLGSAANLIVCEQARRTPQLSYNLSFWNHLKFGVPSTLIVTAI 540
           RAWLLLAWISTVAGNLSLLGSAANLIVCEQARRTPQLSYNLSFWNHLKFGVPSTLIVTAI
Sbjct: 481 RAWLLLAWISTVAGNLSLLGSAANLIVCEQARRTPQLSYNLSFWNHLKFGVPSTLIVTAI 529

Query: 541 GLFLIR 547
           GL LI+
Sbjct: 541 GLVLIK 529

[Close]

BLAST of Lsi05G012130 vs. TrEMBL
Match: M5X637_PRUPE (Uncharacterized protein OS=Prunus persica GN=PRUPE_ppa024761mg PE=4 SV=1)

HSP 1 Score: 780.4 bits (2014), Expect = 1.4e-222
Identity = 413/549 (75.23%), Postives = 476/549 (86.70%), Query Frame = 1


Query: 1   MAMDHTVKVILGSIAFAVFWLLAVFPAIPFLPIGRTAGSLLGAMLMVVFRVLTPDQAYAA 60
           MAM  +VKV+LGSIAFAVFW+LAVFPA+PFLPIGRTAGSLLGAMLMV+FRVLTPDQAYAA
Sbjct: 1   MAMASSVKVVLGSIAFAVFWVLAVFPAVPFLPIGRTAGSLLGAMLMVIFRVLTPDQAYAA 60

Query: 61  IDLPILGLLFGTMVVSVYLERADMFKYLGKVLSWKSKGAKDLICRVCLISAISSAFFTND 120
           IDLPILGLLFGTMVVS+YLERADMFKYLGK+L WK++GAKDL+CR+CLISAISSA FTND
Sbjct: 61  IDLPILGLLFGTMVVSIYLERADMFKYLGKLLLWKTQGAKDLLCRICLISAISSALFTND 120

Query: 121 TSCVVLTEFVLKIARQHNLPPRPFLLALASSANIGSSATPIGNPQNLVIAVQSKIPFGQF 180
           TSCVVLTEFVLK+A+QHNLPP PFLLALASSANIGS+ATPIGNPQNLVIAVQSKIPFG+F
Sbjct: 121 TSCVVLTEFVLKVAKQHNLPPHPFLLALASSANIGSAATPIGNPQNLVIAVQSKIPFGKF 180

Query: 181 VVGILPAMLVGVVVNALIILILYWKLLSVQKDEEDPSPEVVADEDVLSHRFSPARLSHSQ 240
           V+GILPAMLVG+ VNALI+L ++W+LLSV KDEEDP+ EVV +EDV SHRFSPA LSHS 
Sbjct: 181 VIGILPAMLVGIFVNALILLCMFWRLLSVHKDEEDPAGEVVEEEDVSSHRFSPATLSHSA 240

Query: 241 IPSINSAEWDSRLDLMNTQSPPCSNA---NVETLRNSVSSKDNEIHRSHSAMTESARISD 300
             S+NS EW+SRL+  + +S P  +    NVETLRN + S +NEI+  HS   +SAR S 
Sbjct: 241 --SLNSQEWNSRLETADVKSSPNFSRNIDNVETLRNRLPSSENEINNVHSGALDSARNSI 300

Query: 301 ASKEWLPNASPQKREEGFSSKSLNSMDKQKEPVSLQPSEGKEHWSTKWRKIAWKCCVYLV 360
           ASKE + + S  KREE   S+   S +  ++    +  EGKE+ +T+W+++ WK  VYLV
Sbjct: 301 ASKEEIVDVSSHKREETILSERSASNNTLRDACPTESLEGKENLTTRWKRLLWKSGVYLV 360

Query: 361 TIGMLVALLMGLNMSWTAVTAALALVVLDFKDAQPCLEKVSYSLLVFFCGMFMTVDGFNK 420
           TIGML+ALLMGLNMSW A+TAALALVVLDF DA+P LEKVSYSLL+FFCGMF+TV+GFNK
Sbjct: 361 TIGMLIALLMGLNMSWCAITAALALVVLDFTDARPSLEKVSYSLLIFFCGMFITVEGFNK 420

Query: 421 TGLPSAFWNFMEPHAQIDRVSGTVVLALVILYLSNLASNVPTVLLLGARVAASAAAISAA 480
           TG+PSA W+FMEPHA IDR +G  VLAL IL LSNLASNVPTVLLLGARVAASAAAISAA
Sbjct: 421 TGIPSALWDFMEPHAHIDRATGIAVLALTILVLSNLASNVPTVLLLGARVAASAAAISAA 480

Query: 481 QEKRAWLLLAWISTVAGNLSLLGSAANLIVCEQARRTPQLSYNLSFWNHLKFGVPSTLIV 540
           +EK+AWLLLAW+STVAGNLSLLGSAANLIVCEQAR++P L+Y LSFW+HLKFGVPSTL+V
Sbjct: 481 EEKKAWLLLAWVSTVAGNLSLLGSAANLIVCEQARQSPYLAYTLSFWSHLKFGVPSTLLV 540

Query: 541 TAIGLFLIR 547
           TAIGL LIR
Sbjct: 541 TAIGLTLIR 547

[Close]

BLAST of Lsi05G012130 vs. TrEMBL
Match: B9IDB0_POPTR (Uncharacterized protein OS=Populus trichocarpa GN=POPTR_0015s12850g PE=4 SV=2)

HSP 1 Score: 780.4 bits (2014), Expect = 1.4e-222
Identity = 414/550 (75.27%), Postives = 473/550 (86.00%), Query Frame = 1


Query: 1   MAMDHTVKVILGSIAFAVFWLLAVFPAIPFLPIGRTAGSLLGAMLMVVFRVLTPDQAYAA 60
           MAM  TVKV+LG IAFA+FW+LAVFP+IPFLP+GRTAGSLLGAMLMVVFRVLTPDQA+AA
Sbjct: 1   MAMAPTVKVVLGLIAFAIFWVLAVFPSIPFLPVGRTAGSLLGAMLMVVFRVLTPDQAFAA 60

Query: 61  IDLPILGLLFGTMVVSVYLERADMFKYLGKVLSWKSKGAKDLICRVCLISAISSAFFTND 120
           IDLPILGLLFGTMVVSVYLERADMFKYLGK+LSWKSKGAKDL+CR+ +ISAISSA FTND
Sbjct: 61  IDLPILGLLFGTMVVSVYLERADMFKYLGKLLSWKSKGAKDLLCRISVISAISSALFTND 120

Query: 121 TSCVVLTEFVLKIARQHNLPPRPFLLALASSANIGSSATPIGNPQNLVIAVQSKIPFGQF 180
           TSCVVLTEF+LKI RQHNLPP PFLLALASSANIGSSATPIGNPQNLVIAVQSKI FG F
Sbjct: 121 TSCVVLTEFILKITRQHNLPPHPFLLALASSANIGSSATPIGNPQNLVIAVQSKIAFGSF 180

Query: 181 VVGILPAMLVGVVVNALIILILYWKLL-SVQKDEEDPSPEVVADEDVLSHRFSPARLSHS 240
           V GILPAMLVGVVVN LI++ +YWKLL S QKDEED + EVVAD DV+SHRFSPA +SH 
Sbjct: 181 VFGILPAMLVGVVVNILILMCMYWKLLSSAQKDEEDATAEVVADGDVISHRFSPATMSH- 240

Query: 241 QIPSINSAEWDSRLDLMNTQSPPCSNANV---ETLRNSVSSKDNEIHRSHSAMTESARIS 300
            + S+NS EW+SRL+ +N QS P  +  V   ETLRN ++S +NEIH   S++ ESAR S
Sbjct: 241 -LTSLNSLEWNSRLESVNMQSSPNMSGQVNHAETLRNRINSTENEIHSDSSSVYESARNS 300

Query: 301 DASKEWLPNASPQKREEGFSSKSLNSMDKQKEPVSLQPSEGKEHWSTKWRKIAWKCCVYL 360
            ASKE   +AS QKR+E  SS+ + SMD+ ++  S+Q S G   ++TKW+++ WK CVYL
Sbjct: 301 SASKEITNDASSQKRDETVSSRRIESMDRSRDLSSMQFSSG--DFATKWKRMLWKSCVYL 360

Query: 361 VTIGMLVALLMGLNMSWTAVTAALALVVLDFKDAQPCLEKVSYSLLVFFCGMFMTVDGFN 420
           VTIGML+A  +GLNMSWTA+TAALALVVLDFKDAQPC EKVSYSLL+FFCGMF+TVDG N
Sbjct: 361 VTIGMLIAFFLGLNMSWTALTAALALVVLDFKDAQPCFEKVSYSLLIFFCGMFITVDGLN 420

Query: 421 KTGLPSAFWNFMEPHAQIDRVSGTVVLALVILYLSNLASNVPTVLLLGARVAASAAAISA 480
           KTG+PSA W+FMEP+A+ID  +G V+LALVIL LSN ASNVPTVLLLG RVAASAAAISA
Sbjct: 421 KTGIPSALWDFMEPYARIDHTAGIVILALVILVLSNFASNVPTVLLLGGRVAASAAAISA 480

Query: 481 AQEKRAWLLLAWISTVAGNLSLLGSAANLIVCEQARRTPQLSYNLSFWNHLKFGVPSTLI 540
           A EK+AWL+LAW+STV+GNLSLLGSAANLIVCEQA R P   Y+LSFW HLKFGVPST+I
Sbjct: 481 ADEKKAWLILAWVSTVSGNLSLLGSAANLIVCEQAHRAPHFGYSLSFWKHLKFGVPSTII 540

Query: 541 VTAIGLFLIR 547
           +TAIGL LIR
Sbjct: 541 ITAIGLILIR 546

[Close]

BLAST of Lsi05G012130 vs. TAIR10
Match: AT1G02260.1 (AT1G02260.1 Divalent ion symporter)

HSP 1 Score: 564.7 bits (1454), Expect = 6.2e-161
Identity = 325/556 (58.45%), Postives = 395/556 (71.04%), Query Frame = 1


Query: 1   MAMDHTVKVILGSIAFAVFWLLAVFPAIPFLPIGRTAGSLLGAMLMVVFRVLTPDQAYAA 60
           MAM   +K++LGS+AFA+FW+LAVFP++PFLPIGRTAGSL GAMLMV+F+V+TP+QAYAA
Sbjct: 1   MAMAPVIKLVLGSVAFAIFWILAVFPSVPFLPIGRTAGSLFGAMLMVIFQVITPEQAYAA 60

Query: 61  IDLPILGLLFGTMVVSVYLERADMFKYLGKVLSWKSKGAKDLICRVCLISAISSAFFTND 120
           IDLPILGLLFGTMVVS+YLERADMFKYLG +LSWKS+G KDL+CRVCL+SA+SSA FTND
Sbjct: 61  IDLPILGLLFGTMVVSIYLERADMFKYLGTLLSWKSRGPKDLLCRVCLVSAVSSALFTND 120

Query: 121 TSCVVLTEFVLKIARQHNLPPRPFLLALASSANIGSSATPIGNPQNLVIAVQSKIPFGQF 180
           TSCVVLTEFVLKIARQ NLPP PFLLALA+SANIGSSATPIGNPQNLVIAVQSKIP    
Sbjct: 121 TSCVVLTEFVLKIARQKNLPPHPFLLALATSANIGSSATPIGNPQNLVIAVQSKIP---- 180

Query: 181 VVGILPAMLVGVVVNALIILILYWKLLSVQKDEEDPSPEVVADEDVLSHRFSPARLSHSQ 240
                                 +W+ L          P ++    V       A L    
Sbjct: 181 ----------------------FWEFLL------GVFPAMIVGITV------NAMLLLGM 240

Query: 241 IPSINSAEWDSRLDLMNTQSPPCSNANVETLRNS------VSSKDNEIHRSHSAMTESAR 300
              + S   +   ++ N  S   +  +V++ R S      VSS+D+ + R  +A T   R
Sbjct: 241 YWRLLSDHKEDEEEVQNADSEVVAQEDVQSHRFSPATFSPVSSEDSNL-RMDAAETLRNR 300

Query: 301 ISDASKEWL----PNASPQKREEGFSSKSLNSMDKQKEPVSLQPSEGKEHWSTKWRKIAW 360
              A +  L     NAS ++  +  S    N+ +   +             + +WR++ W
Sbjct: 301 AGSAGESELISCNSNASREQHNDAESQGESNNTNNMFQ-------------TKRWRRVLW 360

Query: 361 KCCVYLVTIGMLVALLMGLNMSWTAVTAALALVVLDFKDAQPCLEKVSYSLLVFFCGMFM 420
           K  VY +T+GML++LLMGLNMSWTA+TAALALVVLDFKDA+P LEKVSYSLL+FFCGMF+
Sbjct: 361 KSSVYFITLGMLISLLMGLNMSWTAITAALALVVLDFKDARPSLEKVSYSLLIFFCGMFI 420

Query: 421 TVDGFNKTGLPSAFWNFMEPHAQIDRVSGTVVLALVILYLSNLASNVPTVLLLGARVAAS 480
           TVDGFNKTG+P+A W+ MEP+A+ID+  G  VLA+VIL LSN+ASNVPTVLLLGARVAAS
Sbjct: 421 TVDGFNKTGIPTALWDLMEPYAKIDQAKGIAVLAVVILVLSNVASNVPTVLLLGARVAAS 480

Query: 481 AAAISAAQEKRAWLLLAWISTVAGNLSLLGSAANLIVCEQARRTPQLSYNLSFWNHLKFG 540
             A+   +EK+AWLLLAW+STVAGNL+LLGSAANLIVCEQARR     Y L+F  H KFG
Sbjct: 481 --AMGREEEKKAWLLLAWVSTVAGNLTLLGSAANLIVCEQARRAVSHGYTLTFTKHFKFG 502

Query: 541 VPSTLIVTAIGLFLIR 547
           +PSTLIVTAIGLFLI+
Sbjct: 541 LPSTLIVTAIGLFLIK 502

[Close]

BLAST of Lsi05G012130 vs. NCBI nr
Match: gi|659077548|ref|XP_008439264.1| (PREDICTED: putative transporter arsB [Cucumis melo])

HSP 1 Score: 1006.1 bits (2600), Expect = 2.3e-290
Identity = 522/546 (95.60%), Postives = 537/546 (98.35%), Query Frame = 1


Query: 1   MAMDHTVKVILGSIAFAVFWLLAVFPAIPFLPIGRTAGSLLGAMLMVVFRVLTPDQAYAA 60
           MAMDHTVKVILGSIAFAVFWLLAVFPAIPFLPIGRTAGS+LGAMLMV+FRVLTP+QAYAA
Sbjct: 1   MAMDHTVKVILGSIAFAVFWLLAVFPAIPFLPIGRTAGSILGAMLMVIFRVLTPEQAYAA 60

Query: 61  IDLPILGLLFGTMVVSVYLERADMFKYLGKVLSWKSKGAKDLICRVCLISAISSAFFTND 120
           IDLPILGLLFGTMVVSVYLERADMFKYLGKVLSWKSKGAKDLICRVCLISAISSAFFTND
Sbjct: 61  IDLPILGLLFGTMVVSVYLERADMFKYLGKVLSWKSKGAKDLICRVCLISAISSAFFTND 120

Query: 121 TSCVVLTEFVLKIARQHNLPPRPFLLALASSANIGSSATPIGNPQNLVIAVQSKIPFGQF 180
           TSCVVLTEFVLKIARQHNLPPRPFLLALASSANIGSSATPIGNPQNLVIAVQSKIPFGQF
Sbjct: 121 TSCVVLTEFVLKIARQHNLPPRPFLLALASSANIGSSATPIGNPQNLVIAVQSKIPFGQF 180

Query: 181 VVGILPAMLVGVVVNALIILILYWKLLSVQKDEEDPSPEVVADEDVLSHRFSPARLSHSQ 240
           V+GILPAMLVGVVVNALIILILYWKLLSVQKDEEDPSPEV+ADEDVLSHRFSPARLSHSQ
Sbjct: 181 VIGILPAMLVGVVVNALIILILYWKLLSVQKDEEDPSPEVIADEDVLSHRFSPARLSHSQ 240

Query: 241 IPSINSAEWDSRLDLMNTQSPPCSNANVETLRNSVSSKDNEIHRSHSAMTESARISDASK 300
           IPS+NSAEWDSRLD MN QSPPCSNANVET+RNSVSSKDNEIHRSHS +TE ARISDASK
Sbjct: 241 IPSLNSAEWDSRLDSMNAQSPPCSNANVETIRNSVSSKDNEIHRSHSTLTEPARISDASK 300

Query: 301 EWLPNASPQKREEGFSSKSLNSMDKQKEPVSLQPSEGKEHWSTKWRKIAWKCCVYLVTIG 360
           EWLPNASPQKREEGFSSKSLNSMDKQKEPV LQ SEGKEHWSTKWR+IAWK CVYLVT+G
Sbjct: 301 EWLPNASPQKREEGFSSKSLNSMDKQKEPVILQSSEGKEHWSTKWRRIAWKSCVYLVTVG 360

Query: 361 MLVALLMGLNMSWTAVTAALALVVLDFKDAQPCLEKVSYSLLVFFCGMFMTVDGFNKTGL 420
           ML+ALL+GL+MSWTAVTAALALVVLDFKDAQPCLEKVSYSLLVFFCGMFMTVDGFNKTGL
Sbjct: 361 MLIALLLGLDMSWTAVTAALALVVLDFKDAQPCLEKVSYSLLVFFCGMFMTVDGFNKTGL 420

Query: 421 PSAFWNFMEPHAQIDRVSGTVVLALVILYLSNLASNVPTVLLLGARVAASAAAISAAQEK 480
           PSAFWNFMEPHAQIDRVSGTVVLALVILYLSNLASNVPTVLLLGARVAASAAAIS A+EK
Sbjct: 421 PSAFWNFMEPHAQIDRVSGTVVLALVILYLSNLASNVPTVLLLGARVAASAAAISPAEEK 480

Query: 481 RAWLLLAWISTVAGNLSLLGSAANLIVCEQARRTPQLSYNLSFWNHLKFGVPSTLIVTAI 540
           RAWLLLAWISTVAGNLSLLGSAANLIVCEQARRTPQLSYNLSFWNHLKFG+PSTLIVTAI
Sbjct: 481 RAWLLLAWISTVAGNLSLLGSAANLIVCEQARRTPQLSYNLSFWNHLKFGLPSTLIVTAI 540

Query: 541 GLFLIR 547
           GL LIR
Sbjct: 541 GLVLIR 546

[Close]

BLAST of Lsi05G012130 vs. NCBI nr
Match: gi|449446019|ref|XP_004140769.1| (PREDICTED: putative transporter arsB [Cucumis sativus])

HSP 1 Score: 988.0 bits (2553), Expect = 6.5e-285
Identity = 518/547 (94.70%), Postives = 532/547 (97.26%), Query Frame = 1


Query: 1   MAMDHTVKVILGSIAFAVFWLLAVFPAIPFLPIGRTAGSLLGAMLMVVFRVLTPDQAYAA 60
           MAMDHTVKVILGSIAFAVFWLLAVFPAIPFLPIGRTAGS+LGAMLMVVFRVLTP+QAYAA
Sbjct: 1   MAMDHTVKVILGSIAFAVFWLLAVFPAIPFLPIGRTAGSILGAMLMVVFRVLTPEQAYAA 60

Query: 61  IDLPILGLLFGTMVVSVYLERADMFKYLGKVLSWKSKGAKDLICRVCLISAISSAFFTND 120
           IDLPILGLLFGTMVVSVYLERADMFKYLGKVLSWKSKGAKDLICRVCLISAISSAFFTND
Sbjct: 61  IDLPILGLLFGTMVVSVYLERADMFKYLGKVLSWKSKGAKDLICRVCLISAISSAFFTND 120

Query: 121 TSCVVLTEFVLKIARQHNLPPRPFLLALASSANIGSSATPIGNPQNLVIAVQSKIPFGQF 180
           TSCVVLTEFVLKIARQHNLPPRPFLLALASSANIGSSATPIGNPQNLVIAVQSKI FGQF
Sbjct: 121 TSCVVLTEFVLKIARQHNLPPRPFLLALASSANIGSSATPIGNPQNLVIAVQSKIHFGQF 180

Query: 181 VVGILPAMLVGVVVNALIILILYWKLLSVQKDEEDPSPEVVADEDVLSHRFSPARLSHSQ 240
           V+GILPAMLVGVVVNALIILI+YWKLLSVQKDEEDPSPEV+ADEDVLSHRFSPARLSHSQ
Sbjct: 181 VIGILPAMLVGVVVNALIILIMYWKLLSVQKDEEDPSPEVIADEDVLSHRFSPARLSHSQ 240

Query: 241 IPSINSAEWDSRLDLMNTQSPPCSNANVETLRNSVSSKDN-EIHRSHSAMTESARISDAS 300
           IPS+NSAEWDSRLDLMN QSPPCSN NVET+RNSVSSKDN EI RSHS MTE ARISDAS
Sbjct: 241 IPSLNSAEWDSRLDLMNAQSPPCSNTNVETIRNSVSSKDNEEIRRSHSTMTEPARISDAS 300

Query: 301 KEWLPNASPQKREEGFSSKSLNSMDKQKEPVSLQPSEGKEHWSTKWRKIAWKCCVYLVTI 360
           KEWLPNAS QKREE FSSKSLNSM+KQKEPV LQ SEGKEHWSTKWR+IAWK CVYLVT+
Sbjct: 301 KEWLPNASTQKREEDFSSKSLNSMEKQKEPVILQSSEGKEHWSTKWRRIAWKSCVYLVTV 360

Query: 361 GMLVALLMGLNMSWTAVTAALALVVLDFKDAQPCLEKVSYSLLVFFCGMFMTVDGFNKTG 420
           GMLVALLMGL+MSWTAVTAALALVVLDFKDAQPCLEKVSYSLLVFFCGMFMTVDGFNKTG
Sbjct: 361 GMLVALLMGLDMSWTAVTAALALVVLDFKDAQPCLEKVSYSLLVFFCGMFMTVDGFNKTG 420

Query: 421 LPSAFWNFMEPHAQIDRVSGTVVLALVILYLSNLASNVPTVLLLGARVAASAAAISAAQE 480
           LPSAFWNFMEPHAQIDRVSGTVVLALVILYLSNLASNVPTVLLLGARVAASAAAIS  +E
Sbjct: 421 LPSAFWNFMEPHAQIDRVSGTVVLALVILYLSNLASNVPTVLLLGARVAASAAAISPTEE 480

Query: 481 KRAWLLLAWISTVAGNLSLLGSAANLIVCEQARRTPQLSYNLSFWNHLKFGVPSTLIVTA 540
           KRAWLLLAWISTVAGNLSLLGSAANLIVCEQARRTPQLSYNLSFWNHLKFG+PSTLIVTA
Sbjct: 481 KRAWLLLAWISTVAGNLSLLGSAANLIVCEQARRTPQLSYNLSFWNHLKFGLPSTLIVTA 540

Query: 541 IGLFLIR 547
           IGL LI+
Sbjct: 541 IGLVLIK 547

[Close]

BLAST of Lsi05G012130 vs. NCBI nr
Match: gi|327187684|dbj|BAK09177.1| (silicon efflux transporter CmLsi2-1 [Cucurbita moschata])

HSP 1 Score: 951.8 bits (2459), Expect = 5.1e-274
Identity = 501/549 (91.26%), Postives = 522/549 (95.08%), Query Frame = 1


Query: 1   MAMDHTVKVILGSIAFAVFWLLAVFPAIPFLPIGRTAGSLLGAMLMVVFRVLTPDQAYAA 60
           MAMDHTVKVILGSIAF +FWLLAVFPAIPFLPIGRTAGSLLGAMLMV+FRVLTPDQAYAA
Sbjct: 1   MAMDHTVKVILGSIAFGIFWLLAVFPAIPFLPIGRTAGSLLGAMLMVLFRVLTPDQAYAA 60

Query: 61  IDLPILGLLFGTMVVSVYLERADMFKYLGKVLSWKSKGAKDLICRVCLISAISSAFFTND 120
           IDLPILGLLFGTMVVSVYLERADMFKYLGKVLSWKSKGAKDLICRVCLISAISSA FTND
Sbjct: 61  IDLPILGLLFGTMVVSVYLERADMFKYLGKVLSWKSKGAKDLICRVCLISAISSALFTND 120

Query: 121 TSCVVLTEFVLKIARQHNLPPRPFLLALASSANIGSSATPIGNPQNLVIAVQSKIPFGQF 180
           TSCVVLTEFVLKIA+QHNLPPRPFLLALASSANIGSSATPIGNPQNLVIAVQS IPFGQF
Sbjct: 121 TSCVVLTEFVLKIAKQHNLPPRPFLLALASSANIGSSATPIGNPQNLVIAVQSGIPFGQF 180

Query: 181 VVGILPAMLVGVVVNALIILILYWKLLSVQKDEEDPSPEVVADEDVLSHRFSPARLSHSQ 240
           V+GILPAMLVG+VVNALIILI YWKLLS+QKDEED  PE+VADEDV SHRFSPARLSHSQ
Sbjct: 181 VIGILPAMLVGIVVNALIILISYWKLLSIQKDEEDVPPEIVADEDVNSHRFSPARLSHSQ 240

Query: 241 IPSINSAEWDSRLDLMNTQSPPCSNANVETLRNSVSSKDNEIHRSHSAMTES-ARISDAS 300
           IPS+NS EWDSRLD M  QSPP SNANVETLR S+SSKDNEIH SH A+TE+ ARISDAS
Sbjct: 241 IPSLNSQEWDSRLDSMYAQSPPNSNANVETLRTSLSSKDNEIHWSHGAVTEAVARISDAS 300

Query: 301 KEWLPNAS--PQKREEGFSSKSLNSMDKQKEPVSLQPSEGKEHWSTKWRKIAWKCCVYLV 360
           KE +PNAS  PQKREEG SSKS NSMDKQKEP+ LQ SEGK+HWSTKWR++AWKCCVYLV
Sbjct: 301 KECVPNASPPPQKREEGLSSKSFNSMDKQKEPLPLQVSEGKQHWSTKWRRMAWKCCVYLV 360

Query: 361 TIGMLVALLMGLNMSWTAVTAALALVVLDFKDAQPCLEKVSYSLLVFFCGMFMTVDGFNK 420
           TIGMLVALLMGLNMSWTAVTAALALVVLDFKDAQPCLEKVSYSLLVFFCGMF+TVDGFNK
Sbjct: 361 TIGMLVALLMGLNMSWTAVTAALALVVLDFKDAQPCLEKVSYSLLVFFCGMFITVDGFNK 420

Query: 421 TGLPSAFWNFMEPHAQIDRVSGTVVLALVILYLSNLASNVPTVLLLGARVAASAAAISAA 480
           TGLP+ FWNFMEPHAQIDRVSGTVVLALVILYLSNLASNVPTVLLLGARVAASAAAIS A
Sbjct: 421 TGLPNTFWNFMEPHAQIDRVSGTVVLALVILYLSNLASNVPTVLLLGARVAASAAAISPA 480

Query: 481 QEKRAWLLLAWISTVAGNLSLLGSAANLIVCEQARRTPQLSYNLSFWNHLKFGVPSTLIV 540
           QEKRAWLLLAWISTVAGNLSLLGSAANLIVCEQARRTPQLSYNLSFWNHLKFG+PSTLI+
Sbjct: 481 QEKRAWLLLAWISTVAGNLSLLGSAANLIVCEQARRTPQLSYNLSFWNHLKFGLPSTLII 540

Query: 541 TAIGLFLIR 547
           T IGL LI+
Sbjct: 541 TGIGLVLIK 549

[Close]

BLAST of Lsi05G012130 vs. NCBI nr
Match: gi|327187686|dbj|BAK09178.1| (silicon efflux transporter CmLsi2-2 [Cucurbita moschata])

HSP 1 Score: 932.6 bits (2409), Expect = 3.2e-268
Identity = 501/546 (91.76%), Postives = 514/546 (94.14%), Query Frame = 1


Query: 1   MAMDHTVKVILGSIAFAVFWLLAVFPAIPFLPIGRTAGSLLGAMLMVVFRVLTPDQAYAA 60
           MAMDHTVKVILGSIAFA FWLLAVFPAIPFLP+GRTAGSLLGAMLMVVFRVLTPDQAYAA
Sbjct: 1   MAMDHTVKVILGSIAFATFWLLAVFPAIPFLPVGRTAGSLLGAMLMVVFRVLTPDQAYAA 60

Query: 61  IDLPILGLLFGTMVVSVYLERADMFKYLGKVLSWKSKGAKDLICRVCLISAISSAFFTND 120
           IDLPILGLLFGTMVVSVYLERADMFKYLGKVLSW+SKGAKDLICRVCLISAISSAFFTND
Sbjct: 61  IDLPILGLLFGTMVVSVYLERADMFKYLGKVLSWRSKGAKDLICRVCLISAISSAFFTND 120

Query: 121 TSCVVLTEFVLKIARQHNLPPRPFLLALASSANIGSSATPIGNPQNLVIAVQSKIPFGQF 180
           TSCVVLTEFVLKIARQHNLPPRPFLLALASSANIGSSATPIGNPQNLVIAVQSKIPFG+F
Sbjct: 121 TSCVVLTEFVLKIARQHNLPPRPFLLALASSANIGSSATPIGNPQNLVIAVQSKIPFGEF 180

Query: 181 VVGILPAMLVGVVVNALIILILYWKLLSVQKDEEDPSPEVVADEDVLSHRFSPARLSHSQ 240
           VVGILPAMLVGVVVNALIILILYWKLLSVQKDEEDPS E+VADE VLSHRFSPARLSH+Q
Sbjct: 181 VVGILPAMLVGVVVNALIILILYWKLLSVQKDEEDPSSEIVADEVVLSHRFSPARLSHTQ 240

Query: 241 IPSINSAEWDSRLDLMNTQSPPCSNANVETLRNSVSSKDNEIHRSHSAMTESARISDASK 300
           IPS+NS EW+SRLD M        NANVET R SVSSK+NEIHRSHSA+TESARISDAS 
Sbjct: 241 IPSLNSPEWESRLDSM--------NANVETQR-SVSSKENEIHRSHSALTESARISDASS 300

Query: 301 EWLPNASPQKREEGFSSKSLNSMDKQKEPVSLQPSEGKEHWSTKWRKIAWKCCVYLVTIG 360
                    KREEGFSSKSLNSMDKQKE VSL  SE KEHWSTKWR+IAWKCCVYLVTIG
Sbjct: 301 --------LKREEGFSSKSLNSMDKQKESVSLPSSEEKEHWSTKWRRIAWKCCVYLVTIG 360

Query: 361 MLVALLMGLNMSWTAVTAALALVVLDFKDAQPCLEKVSYSLLVFFCGMFMTVDGFNKTGL 420
           MLVALLMGLNMSWTAVTAALALVVLDFKDAQPCLEKVSYSLLVFFCGMFMTVDGFNKTGL
Sbjct: 361 MLVALLMGLNMSWTAVTAALALVVLDFKDAQPCLEKVSYSLLVFFCGMFMTVDGFNKTGL 420

Query: 421 PSAFWNFMEPHAQIDRVSGTVVLALVILYLSNLASNVPTVLLLGARVAASAAAISAAQEK 480
           PSAFW+FMEPHAQIDRVSGTVVLALVILYLSNLASNVPTVLLLGARVAASAAAIS  +EK
Sbjct: 421 PSAFWDFMEPHAQIDRVSGTVVLALVILYLSNLASNVPTVLLLGARVAASAAAISPMEEK 480

Query: 481 RAWLLLAWISTVAGNLSLLGSAANLIVCEQARRTPQLSYNLSFWNHLKFGVPSTLIVTAI 540
           RAWLLLAWISTVAGNLSLLGSAANLIVCEQARRTPQLSYNLSFWNHLKFGVPSTLIVTAI
Sbjct: 481 RAWLLLAWISTVAGNLSLLGSAANLIVCEQARRTPQLSYNLSFWNHLKFGVPSTLIVTAI 529

Query: 541 GLFLIR 547
           GL LI+
Sbjct: 541 GLVLIK 529

[Close]

BLAST of Lsi05G012130 vs. NCBI nr
Match: gi|566207314|ref|XP_002322484.2| (hypothetical protein POPTR_0015s12850g [Populus trichocarpa])

HSP 1 Score: 780.4 bits (2014), Expect = 2.0e-222
Identity = 414/550 (75.27%), Postives = 473/550 (86.00%), Query Frame = 1


Query: 1   MAMDHTVKVILGSIAFAVFWLLAVFPAIPFLPIGRTAGSLLGAMLMVVFRVLTPDQAYAA 60
           MAM  TVKV+LG IAFA+FW+LAVFP+IPFLP+GRTAGSLLGAMLMVVFRVLTPDQA+AA
Sbjct: 1   MAMAPTVKVVLGLIAFAIFWVLAVFPSIPFLPVGRTAGSLLGAMLMVVFRVLTPDQAFAA 60

Query: 61  IDLPILGLLFGTMVVSVYLERADMFKYLGKVLSWKSKGAKDLICRVCLISAISSAFFTND 120
           IDLPILGLLFGTMVVSVYLERADMFKYLGK+LSWKSKGAKDL+CR+ +ISAISSA FTND
Sbjct: 61  IDLPILGLLFGTMVVSVYLERADMFKYLGKLLSWKSKGAKDLLCRISVISAISSALFTND 120

Query: 121 TSCVVLTEFVLKIARQHNLPPRPFLLALASSANIGSSATPIGNPQNLVIAVQSKIPFGQF 180
           TSCVVLTEF+LKI RQHNLPP PFLLALASSANIGSSATPIGNPQNLVIAVQSKI FG F
Sbjct: 121 TSCVVLTEFILKITRQHNLPPHPFLLALASSANIGSSATPIGNPQNLVIAVQSKIAFGSF 180

Query: 181 VVGILPAMLVGVVVNALIILILYWKLL-SVQKDEEDPSPEVVADEDVLSHRFSPARLSHS 240
           V GILPAMLVGVVVN LI++ +YWKLL S QKDEED + EVVAD DV+SHRFSPA +SH 
Sbjct: 181 VFGILPAMLVGVVVNILILMCMYWKLLSSAQKDEEDATAEVVADGDVISHRFSPATMSH- 240

Query: 241 QIPSINSAEWDSRLDLMNTQSPPCSNANV---ETLRNSVSSKDNEIHRSHSAMTESARIS 300
            + S+NS EW+SRL+ +N QS P  +  V   ETLRN ++S +NEIH   S++ ESAR S
Sbjct: 241 -LTSLNSLEWNSRLESVNMQSSPNMSGQVNHAETLRNRINSTENEIHSDSSSVYESARNS 300

Query: 301 DASKEWLPNASPQKREEGFSSKSLNSMDKQKEPVSLQPSEGKEHWSTKWRKIAWKCCVYL 360
            ASKE   +AS QKR+E  SS+ + SMD+ ++  S+Q S G   ++TKW+++ WK CVYL
Sbjct: 301 SASKEITNDASSQKRDETVSSRRIESMDRSRDLSSMQFSSG--DFATKWKRMLWKSCVYL 360

Query: 361 VTIGMLVALLMGLNMSWTAVTAALALVVLDFKDAQPCLEKVSYSLLVFFCGMFMTVDGFN 420
           VTIGML+A  +GLNMSWTA+TAALALVVLDFKDAQPC EKVSYSLL+FFCGMF+TVDG N
Sbjct: 361 VTIGMLIAFFLGLNMSWTALTAALALVVLDFKDAQPCFEKVSYSLLIFFCGMFITVDGLN 420

Query: 421 KTGLPSAFWNFMEPHAQIDRVSGTVVLALVILYLSNLASNVPTVLLLGARVAASAAAISA 480
           KTG+PSA W+FMEP+A+ID  +G V+LALVIL LSN ASNVPTVLLLG RVAASAAAISA
Sbjct: 421 KTGIPSALWDFMEPYARIDHTAGIVILALVILVLSNFASNVPTVLLLGGRVAASAAAISA 480

Query: 481 AQEKRAWLLLAWISTVAGNLSLLGSAANLIVCEQARRTPQLSYNLSFWNHLKFGVPSTLI 540
           A EK+AWL+LAW+STV+GNLSLLGSAANLIVCEQA R P   Y+LSFW HLKFGVPST+I
Sbjct: 481 ADEKKAWLILAWVSTVSGNLSLLGSAANLIVCEQAHRAPHFGYSLSFWKHLKFGVPSTII 540

Query: 541 VTAIGLFLIR 547
           +TAIGL LIR
Sbjct: 541 ITAIGLILIR 546

The following BLAST results are available for this feature:

BLAST of Lsi05G012130 vs. Swiss-Prot


Match Name	E-value	Identity	Description
ARSB_DICDI	2.7e-57	31.69	Putative transporter arsB OS=Dictyostelium discoideum GN=arsB PE=2 SV=1	[more]
P_PIG	1.8e-16	23.73	P protein OS=Sus scrofa GN=Oca2 PE=2 SV=3	[more]
P_HUMAN	4.8e-14	23.32	P protein OS=Homo sapiens GN=OCA2 PE=1 SV=2	[more]
P_MOUSE	1.4e-13	23.53	P protein OS=Mus musculus GN=Oca2 PE=1 SV=1	[more]
Y2685_MYCTO	2.9e-11	28.89	Uncharacterized transporter MT2759 OS=Mycobacterium tuberculosis (strain CDC 155...	[more]

BLAST of Lsi05G012130 vs. TrEMBL


Match Name	E-value	Identity	Description
A0A0A0L6L7_CUCSA	4.5e-285	94.70	Uncharacterized protein OS=Cucumis sativus GN=Csa_3G182780 PE=4 SV=1	[more]
F1SX52_CUCMO	3.6e-274	91.26	Silicon efflux transporter CmLsi2-1 OS=Cucurbita moschata GN=CmLsi2-1 PE=2 SV=1	[more]
F1SX53_CUCMO	2.2e-268	91.76	Silicon efflux transporter CmLsi2-2 OS=Cucurbita moschata GN=CmLsi2-2 PE=2 SV=1	[more]
M5X637_PRUPE	1.4e-222	75.23	Uncharacterized protein OS=Prunus persica GN=PRUPE_ppa024761mg PE=4 SV=1	[more]
B9IDB0_POPTR	1.4e-222	75.27	Uncharacterized protein OS=Populus trichocarpa GN=POPTR_0015s12850g PE=4 SV=2	[more]

BLAST of Lsi05G012130 vs. TAIR10


Match Name	E-value	Identity	Description
AT1G02260.1	6.2e-161	58.45	Divalent ion symporter	[more]

BLAST of Lsi05G012130 vs. NCBI nr


Match Name	E-value	Identity	Description
gi\|659077548\|ref\|XP_008439264.1\|	2.3e-290	95.60	PREDICTED: putative transporter arsB [Cucumis melo]	[more]
gi\|449446019\|ref\|XP_004140769.1\|	6.5e-285	94.70	PREDICTED: putative transporter arsB [Cucumis sativus]	[more]
gi\|327187684\|dbj\|BAK09177.1\|	5.1e-274	91.26	silicon efflux transporter CmLsi2-1 [Cucurbita moschata]	[more]
gi\|327187686\|dbj\|BAK09178.1\|	3.2e-268	91.76	silicon efflux transporter CmLsi2-2 [Cucurbita moschata]	[more]
gi\|566207314\|ref\|XP_002322484.2\|	2.0e-222	75.27	hypothetical protein POPTR_0015s12850g [Populus trichocarpa]	[more]

Annotated Terms

The following terms have been associated with this gene:

Vocabulary: **Biological Process**
Term	Definition
GO:0055085	transmembrane transport

Vocabulary: **Cellular Component**
Term	Definition
GO:0016021	integral component of membrane

Vocabulary: **INTERPRO**
Term	Definition
IPR004680	Cit_transptr-like_dom

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0098656	anion transmembrane transport
biological_process	GO:0055085	transmembrane transport
cellular_component	GO:0016021	integral component of membrane
molecular_function	GO:0005215	transporter activity
molecular_function	GO:0003674	molecular_function

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Lsi05G012130.1	Lsi05G012130.1	mRNA

InterPro

Analysis Name: InterPro Annotations of Lagenaria siceraria
Date Performed: 2017-09-18


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR004680	Citrate transporter-like domain	PFAM	PF03600	CitMHS	coord: 29..493 score: 5.9
None	No IPR available	PANTHER	PTHR10283	SOLUTE CARRIER FAMILY 13 MEMBER	coord: 1..225 score: 1.4E-287coord: 300..545 score: 1.4E
None	No IPR available	PANTHER	PTHR10283:SF94	SUBFAMILY NOT NAMED	coord: 1..225 score: 1.4E-287coord: 300..545 score: 1.4E

Synteny

The following gene(s) are orthologous to this gene:


Gene	Orthologue	Organism	Block
Lsi05G012130	Csa3G182780	Cucumber (Chinese Long) v2	culsiB210
Lsi05G012130	CSPI03G17100	Wild cucumber (PI 183967)	cpilsiB208
Lsi05G012130	Cucsa.107960	Cucumber (Gy14) v1	cgylsiB143
Lsi05G012130	CmaCh16G001130	Cucurbita maxima (Rimu)	cmalsiB312
Lsi05G012130	CmaCh18G012580	Cucurbita maxima (Rimu)	cmalsiB387
Lsi05G012130	CmoCh18G012850	Cucurbita moschata (Rifu)	cmolsiB372
Lsi05G012130	CmoCh16G001250	Cucurbita moschata (Rifu)	cmolsiB300
Lsi05G012130	Cla006164	Watermelon (97103) v1	lsiwmB366
Lsi05G012130	MELO3C006926	Melon (DHL92) v3.5.1	lsimeB379
Lsi05G012130	ClCG05G008200	Watermelon (Charleston Gray)	lsiwcgB336
Lsi05G012130	MELO3C006926.2	Melon (DHL92) v3.6.1	lsimedB397
Lsi05G012130	Cla97C05G090280	Watermelon (97103) v2	lsiwmbB334
Lsi05G012130	Bhi01G000940	Wax gourd	lsiwgoB434
Lsi05G012130	Cp4.1LG14g06260	Cucurbita pepo (Zucchini)	cpelsiB172
Lsi05G012130	Cp4.1LG09g00720	Cucurbita pepo (Zucchini)	cpelsiB035
Lsi05G012130	CsGy3G017160	Cucumber (Gy14) v2	cgyblsiB197
Lsi05G012130	Carg22116	Silver-seed gourd	carlsiB675
Lsi05G012130	Carg15123	Silver-seed gourd	carlsiB903
Lsi05G012130	CsaV3_3G017280	Cucumber (Chinese Long) v3	cuclsiB229

The following gene(s) are paralogous to this gene:

None

The following block(s) are covering this gene:


Gene	Organism	Block
Lsi05G012130	Cucurbita pepo (Zucchini)	cpelsiB618
Lsi05G012130	Cucumber (Gy14) v2	cgyblsiB193
Lsi05G012130	Melon (DHL92) v3.6.1	lsimedB394
Lsi05G012130	Silver-seed gourd	carlsiB650
Lsi05G012130	Watermelon (97103) v2	lsiwmbB331
Lsi05G012130	Bottle gourd (USVL1VR-Ls)	lsilsiB160
Lsi05G012130	Cucurbita maxima (Rimu)	cmalsiB587
Lsi05G012130	Cucurbita maxima (Rimu)	cmalsiB836
Lsi05G012130	Cucurbita moschata (Rifu)	cmolsiB567