Cucsa.107960 (gene) Cucumber (Gy14) v1

BLAST of Cucsa.107960 vs. Swiss-Prot
Match: ARSB_DICDI (Putative transporter arsB OS=Dictyostelium discoideum GN=arsB PE=2 SV=1)

HSP 1 Score: 252.7 bits (644), Expect = 9.3e-66
Identity = 165/527 (31.31%), Postives = 279/527 (52.94%), Query Frame = 1

Query: 31  LPIGRTAGSILGAMLMVVFRVLTPEQAYAAIDLPILGLLFGTMVVSVYLERADMFKYLGK 90
           LPIGR   SI+GA LMV F ++ P++  + I+   + LL   M++S Y+E+A+++    K
Sbjct: 66  LPIGRAGSSIIGATLMVYFGIIQPKEIGSVINWDTIILLMSMMMLSNYMEQANIWGMASK 125

Query: 91  VLSWKSKGAKDLICRVCLISAISSAFFTNDTSCVVLTEFVLKIARQHNLPPRPFLLALAS 150
           +L WK K     + RVCLIS+I S+  TNDT CV LT  V+   +  NL   PFL+A+A+
Sbjct: 126 ILLWKCKSTSIFMVRVCLISSIMSSILTNDTVCVTLTPIVISACKSTNLTFFPFLMAIAT 185

Query: 151 SANIGSSATPIGNPQNLVIAVQSKIPFGQFVIGILPAMLVGVVVNALIILIMYWKLLSVQ 210
           SANIGSSA P+GNPQN++IA    + F  F    + + ++GV +N +++L+ + K L   
Sbjct: 186 SANIGSSALPVGNPQNMIIATAGGLNFFNFFKVSIVSSILGVCLNTILLLLYFKKDLKNL 245

Query: 211 KDE-----EDPSPEVIADEDVLSHRFSPARLSHSQIPSLNSAEWDSRLDLMNAQSPPCSN 270
                   E  +P+V   E++ ++        H    + N ++ +  ++ +N +     +
Sbjct: 246 NSNFNQLIETVNPKV---EEIDNNH-------HDDDGANNQSKNEKEMENINKEVEEEQH 305

Query: 271 TNVETIRNSVSSKDNEEIRRSHSTMTEPARIS--DASKEWLPNASTQKREEDFSSKSLNS 330
            N +   +  +   N      H+ +   + +   D S   + N   +K++E+F     NS
Sbjct: 306 NNDDDDDDGFNENKNNNNNGGHAILLVASSMDSIDLSDCSIIN-KDKKKKENFIEIYFNS 365

Query: 331 MEKQKEPVILQSSEGKEHWSTKWRRIAWKSCVYLVTVGMLVALLMGLDMSWTAVTAALAL 390
            EK  E ++               ++ +K  V ++   +L+   +G+ M +T +     L
Sbjct: 366 KEKSIETIV------------NIIKLIFKFRVAIILTLVLIGFFIGMHMGFTVLFGVSIL 425

Query: 391 VVLDFKDAQPCLEKVSYSLLVFFCGMFMTVDGFNKTGLPSAFWNFMEPHAQID----RVS 450
           ++ + KD    +  V + LL+FF G+F+ V+GF++     A W  +EP   ID     V 
Sbjct: 426 MICERKDITDIINSVDWELLLFFSGLFVLVEGFDRQFEKEA-WTILEPFVPIDSTHLNVL 485

Query: 451 GTVVLALVILYLSNLASNVPTVLLLGARVAASAAAISPTEEKRAWLLLAWISTVAGNLSL 510
              + +++IL L N+  NVP VL L  R+  + A          W+LLA++STVAGNL+L
Sbjct: 486 KIFIFSILILVLCNILGNVPLVLSLSPRLLEALA------PDFTWILLAFVSTVAGNLTL 545

Query: 511 LGSAANLIVCEQARRTPQLSYNLSFWNHLKFGLPSTLIVTAIGLVLI 547
           +GS ANLIV E+++      + + F  +LKFG+PST++V  IG+ ++
Sbjct: 546 VGSVANLIVAEKSKS----YHEIGFLEYLKFGVPSTILVILIGVPIV 558

BLAST of Cucsa.107960 vs. Swiss-Prot
Match: P_PIG (P protein OS=Sus scrofa GN=Oca2 PE=2 SV=3)

HSP 1 Score: 113.2 bits (282), Expect = 8.8e-24
Identity = 135/568 (23.77%), Postives = 224/568 (39.44%), Query Frame = 1

Query: 1   MAMDHTVKVILGSIAFAVFWLLAVFPAIPFLPIGRTAGSILGAM-----LMVVFRVLTPE 60
           MA  +    I   +  A   L  V+  I F  + RT  ++LG++     L V+    T  
Sbjct: 325 MAHQYLRASIEAQVTIAAVILAGVYVLIIFEIVHRTLAAMLGSLAALAALAVIGDRPTLT 384

Query: 61  QAYAAIDLPILGLLFGTMVVSVYLERADMFKYLGKVLSWKSKGAK-DLICRVCLISAISS 120
           Q    ID   L LLFG M++         F Y        S+G    +I  +CLI+A+ S
Sbjct: 385 QVVEWIDFETLALLFGMMILVAIFSETGFFDYCAVKAYQLSRGRVWAMIIMLCLIAAVLS 444

Query: 121 AFFTNDTSCVVLTEFVLKIARQHNLPPRPFLLALASSANIGSSATPIGNPQNLVIAVQSK 180
           AF  N T+ ++ T   +++    NL PR  L+A     NIG +AT IG+P N++I    +
Sbjct: 445 AFLDNVTTALLFTPVTIRLCEVLNLDPRQVLIAEVIFTNIGGAATAIGDPPNVIIVSNQE 504

Query: 181 I-PFGQFVIGILPAMLVGVVVNAL----IILIMYWKLLSVQKDEEDPSPEVIADEDVLSH 240
           +   G    G    M  G+    L    ++ ++YW      K+     P  I +   L H
Sbjct: 505 LRKMGLDFAGFTAHMFAGICFVLLFSFPLLRLLYWNRKLYNKE-----PSEIVE---LKH 564

Query: 241 RFSPARLSHSQIPSLNSAEWDSRLDLMNAQSPPCSNTNVETIRNSVSSKDNEEIRRSHST 300
                RL+  +I   +  E   R  L+                  V S +    RR HS 
Sbjct: 565 EIHVWRLTAQRISPASREETAVRGLLL----------------EKVLSLERLLARRLHSF 624

Query: 301 MTEPARISDASKEWLPNASTQKREEDFSSKSLNSMEKQKEPVILQSSEGKEHWSTKWRRI 360
             +   IS   K W  N    +++   S ++L +                          
Sbjct: 625 HRQ---ISQEDKNWETNIQELQKKHRISDRTLLT-------------------------- 684

Query: 361 AWKSCVYLVTVGMLVALL------MGLDMSWTAVTAALALVVL-DFKDAQPCLEKVSYSL 420
               CV ++ + + +  L      + LD+ W A+  A+ L++L D  D +  L +V ++ 
Sbjct: 685 ---KCVTVLGLVIFMFFLNSFVPGVHLDLGWIAILGAIWLLILADIHDFEIILHRVEWAT 744

Query: 421 LVFFCGMFMTVDG------FNKTGLPSAFWNFMEPHAQIDRVSGTVVLALVILYLSNLAS 480
           L+FF  +F+ ++           G  +A    M P  Q    +  +V+  V    S+L  
Sbjct: 745 LLFFAALFILMEALAHLHLIEYVGEQTALLIKMVPEDQ-RLAAAIIVVVWVSAIASSLID 804

Query: 481 NVP-TVLLLGARVAASAAAISPTEEKRAWLLLAWISTVAGNLSLLGSAANLIVCEQARRT 540
           N+P T  ++   +  S               LA  + + GN +L+G++AN +VC  A   
Sbjct: 805 NIPFTATMIPVLLNLSRDPEISLPAPPLMYALALGACLGGNGTLIGASAN-VVC--AGIA 832

Query: 541 PQLSYNLSFWNHLKFGLPSTLIVTAIGL 544
            Q  Y  SF    + G P  ++   +G+
Sbjct: 865 EQHGYGFSFMEFFRLGFPMMVVSCMVGM 832

BLAST of Cucsa.107960 vs. Swiss-Prot
Match: P_MOUSE (P protein OS=Mus musculus GN=Oca2 PE=1 SV=1)

HSP 1 Score: 104.8 bits (260), Expect = 3.1e-21
Identity = 128/546 (23.44%), Postives = 223/546 (40.84%), Query Frame = 1

Query: 14  IAFAVFWLLAVFPAIPFLPIGRTAGSILGAMLMVVFRVLTPEQAYAA-----IDLPILGL 73
           +A AV  L  V+  I F  + RT  ++LGA+  +    +  ++         ID   L L
Sbjct: 326 VASAVAILAGVYTLIIFEIVHRTLAAMLGALAALAALAVVGDRPSLTHVVEWIDFETLAL 385

Query: 74  LFGTMVVSVYLERADMFKYLGKVLSWKSKGAK-DLICRVCLISAISSAFFTNDTSCVVLT 133
           LFG M++         F Y        S+G    +I  +CL++AI SAF  N T+ ++ T
Sbjct: 386 LFGMMILVAVFSETGFFDYCAVKAYQLSRGRVWAMIFMLCLMAAILSAFLDNVTTMLLFT 445

Query: 134 EFVLKIARQHNLPPRPFLLALASSANIGSSATPIGNPQNLVIAVQSKI-PFGQFVIGILP 193
              +++    NL PR  L+A     NIG +AT IG+P N++I    ++   G    G   
Sbjct: 446 PVTIRLCEVLNLDPRQVLIAEVIFTNIGGAATAIGDPPNVIIVSNQELRKMGLDFAGFTA 505

Query: 194 AMLVGVVVNALIILIMYWKLLSVQKDEEDPSPEVIADEDVLSHRFSPARLSHSQIPSLNS 253
            M +G+ +  L++     +LL   K   +  P  I +   L H     RL+  +I   + 
Sbjct: 506 HMFLGICL-VLLVSFPLLRLLYWNKKLYNKEPSEIVE---LKHEIHVWRLTAQRISPASR 565

Query: 254 AEWDSRLDLMNAQSPPCSNTNVETIRNSVSSKDNEEIRRSHSTMTEPARISDASKEWLPN 313
            E   R  L+                  V + ++   +R H   T   +IS   K W  N
Sbjct: 566 EETAVRGLLL----------------EKVLALEHLLAQRLH---TFHRQISQEDKNWETN 625

Query: 314 ASTQKREEDFSSKSLNSMEKQKEPVILQSSEGKEHWSTKWRRIAWKSCVYLVTVGMLVAL 373
               +R+   S +SL          +++         + +   ++   ++L         
Sbjct: 626 IQELQRKHRISDRSL----------LVKCLTVLGFVISMFFLNSFVPGIHL--------- 685

Query: 374 LMGLDMSWTAVTAALALVVL-DFKDAQPCLEKVSYSLLVFFCGMFMTVDGFNK------T 433
               D+ W A+  A+ L++L D  D +  L +V ++ L+FF  +F+ ++           
Sbjct: 686 ----DLGWIAILGAIWLLILADIHDFEIILHRVEWATLLFFAALFVLMEALTHLHLVEYV 745

Query: 434 GLPSAFWNFMEPHAQIDRVSGTVVLALVILYL-SNLASNVP-TVLLLGARVAASAAAISP 493
           G  +A    M P  Q  R +  +VL + +  L S+L  N+P T  ++   +  S      
Sbjct: 746 GEQTALLIKMVPEDQ--RFAAAIVLIVWVSALASSLIDNIPFTATMIPVLLNLSQDPEIS 805

Query: 494 TEEKRAWLLLAWISTVAGNLSLLGSAANLIVCEQARRTPQLSYNLSFWNHLKFGLPSTLI 544
                    LA  + + GN +L+G++ N++    A +     Y  SF    + G P  L+
Sbjct: 806 LPALPLMYALALGACLGGNGTLIGASTNVVCAGIAEKH---GYGFSFMEFFRLGFPVMLM 820

BLAST of Cucsa.107960 vs. Swiss-Prot
Match: AG45_MYCLE (46 kDa membrane protein OS=Mycobacterium leprae (strain TN) GN=ag45 PE=3 SV=2)

HSP 1 Score: 81.3 bits (199), Expect = 3.7e-14
Identity = 54/222 (24.32%), Postives = 111/222 (50.00%), Query Frame = 1

Query: 14  IAFAVFWLLAVFPAIPFLPIGRTAGSILGAMLMVVFRVLTPEQAY----AAIDLPILGLL 73
           IA  VF  +  +  I    + +T  ++ GA ++V   ++  E  +      ID  ++ LL
Sbjct: 4   IAITVF--IVTYALIASDRVNKTLAALTGAAIVVTLPIINSEDVFYSHETGIDWEVIFLL 63

Query: 74  FGTMVVSVYLERADMFKYLGKVLSWKSKGAK-DLICRVCLISAISSAFFTNDTSCVVLTE 133
              M++   L +  +F+Y+    + +S G+   ++  + L+ A+ SA   N T+ +++  
Sbjct: 64  LSMMIIVSVLRQTGVFEYVAIWTAKRSHGSPLRILLLLVLVMALGSALLDNVTTVLLIAP 123

Query: 134 FVLKIARQHNLPPRPFLLALASSANIGSSATPIGNPQNLVIAVQSKIPFGQFVIGILPAM 193
             L +  +  +   PFL+A   ++NIG +AT +G+P N++IA ++   F  F+I + P +
Sbjct: 124 VTLLVCERLTINAAPFLMAEVFASNIGGAATLVGDPPNIIIASRAGFSFNDFLIHLTPIV 183

Query: 194 LVGVVVNALIILIMYWKLLSVQKDEEDPSPEVIADEDVLSHR 231
           ++  VV + ++  ++    +V        PE +AD   L+ R
Sbjct: 184 IIVTVVLSALLPRLFRGAFAV-------DPERVADIMSLNER 216

HSP 2 Score: 56.2 bits (134), Expect = 1.3e-06
Identity = 56/217 (25.81%), Postives = 95/217 (43.78%), Query Frame = 1

Query: 333 LQSSEGKEHWSTKWRRIAWKSCVYLVTVGMLVALLMGLDMSWTAVTAALALVVLDFKDAQ 392
           + S   +E    +W  I     + LV V  +   ++    S   +  A  L+V+   +  
Sbjct: 210 IMSLNEREAIRDRWLLIKCGVVLLLVFVAFIAHPVLHTGPSLVGMLGAGILIVISKLERS 269

Query: 393 PCLEKVSYSLLVFFCGMFMTVDGFNKTG----LPSAFWNFMEPHAQIDRVSGTVVLAL-V 452
             L  V +  L+FF G+F+ V    KT     L  A       H  +     TVVL L V
Sbjct: 270 DYLSSVKWETLLFFAGLFIMVGALVKTDVVNQLARATTTLTGGHELL-----TVVLTLGV 329

Query: 453 ILYLSNLASNVPTVLLLGARVAASAAAI-SPTEEKRAWLLLAWISTVAGNLSLLGSAANL 512
              +S++  N+P V  +   V+   A++   +     W  LA  +   GNL+ +G++AN+
Sbjct: 330 STLVSSIIDNIPYVATMTPIVSELVASMPDQSHTDILWWALALGADFGGNLTAVGASANV 389

Query: 513 IVCEQARR--TPQLSYNLSFWNHLKFGLPSTLIVTAI 542
           ++ E A+   TP     +SFW   + G+  T+I  A+
Sbjct: 390 VMLEIAKSAGTP-----ISFWEFTRKGIAVTVISIAL 416

BLAST of Cucsa.107960 vs. Swiss-Prot
Match: Y2703_MYCBO (Uncharacterized transporter Mb2703 OS=Mycobacterium bovis (strain ATCC BAA-935 / AF2122/97) GN=Mb2703 PE=3 SV=1)

HSP 1 Score: 80.1 bits (196), Expect = 8.3e-14
Identity = 54/193 (27.98%), Postives = 102/193 (52.85%), Query Frame = 1

Query: 17  AVFWLLAVFPAIPFLPIGRTAGSILGAMLMVVFRVLTPEQAY----AAIDLPILGLLFGT 76
           AV   +A +  I    + +T  ++ GA  +VV  V+T    +      ID  ++ LL G 
Sbjct: 5   AVTIFVAAYVLIASDRVNKTMVALTGAAAVVVLPVITSHDIFYSHDTGIDWDVIFLLVGM 64

Query: 77  MVVSVYLERADMFKYLGKVLSWKSKGAKDLICRVC----LISAISSAFFTNDTSCVVLTE 136
           M++   L +  +F+Y      W +K A+    R+     L+SA++SA   N T+ +++  
Sbjct: 65  MIIVGVLRQTGVFEYTA---IWAAKRARGSPLRIMILLVLVSALASALLDNVTTVLLIAP 124

Query: 137 FVLKIARQHNLPPRPFLLALASSANIGSSATPIGNPQNLVIAVQSKIPFGQFVIGILPAM 196
             L +  + N+    FL+A   ++NIG +AT +G+P N+++A ++ + F  F++ + P  
Sbjct: 125 VTLLVCDRLNINTTSFLMAEVFASNIGGAATLVGDPPNIIVASRAGLTFNDFMLHLTP-- 184

Query: 197 LVGVVVNALIILI 202
           LV +V+ ALI ++
Sbjct: 185 LVVIVLIALIAVL 192

HSP 2 Score: 55.1 bits (131), Expect = 2.9e-06
Identity = 48/195 (24.62%), Postives = 82/195 (42.05%), Query Frame = 1

Query: 357 LVTVGMLVALLMGLDMSWTAVTAALALVVLDFKDAQPCLEKVSYSLLVFFCGMFMTVDGF 416
           LV    +   ++ +  S  A+  A  L+V+        L  V +  L+FF G+F+ V   
Sbjct: 233 LVFAAFVAHPVLHIQPSLVALLGAGMLIVVSGLTRSEYLSSVEWDTLLFFAGLFIMVGAL 292

Query: 417 NKTGLPSAFWNFMEPHAQIDRVSGTVVLALVILY-----LSNLASNVPTVLLLGARVAAS 476
            KTG+ +         A      G +V    ++      +S +  N+P V  +   VA  
Sbjct: 293 VKTGVVNDL-----ARAATQLTGGNIVATAFLILGVSAPISGIIDNIPYVATMTPLVAEL 352

Query: 477 AAAIS-PTEEKRAWLLLAWISTVAGNLSLLGSAANLIVCEQARRTPQLSYNLSFWNHLKF 536
            A +         W  LA  +   GNL+ +G++AN+++   ARR       +SFW   + 
Sbjct: 353 VAVMGGQPSTDTPWWALALGADFGGNLTAIGASANVVMLGIARRA---GAPISFWEFTRK 412

Query: 537 GLPSTLIVTAIGLVL 546
           G     +VTA+ + L
Sbjct: 413 G----AVVTAVSIAL 415

BLAST of Cucsa.107960 vs. TrEMBL
Match: A0A0A0L6L7_CUCSA (Uncharacterized protein OS=Cucumis sativus GN=Csa_3G182780 PE=4 SV=1)

HSP 1 Score: 1067.0 bits (2758), Expect = 7.7e-309
Identity = 546/547 (99.82%), Postives = 546/547 (99.82%), Query Frame = 1

Query: 1   MAMDHTVKVILGSIAFAVFWLLAVFPAIPFLPIGRTAGSILGAMLMVVFRVLTPEQAYAA 60
           MAMDHTVKVILGSIAFAVFWLLAVFPAIPFLPIGRTAGSILGAMLMVVFRVLTPEQAYAA
Sbjct: 1   MAMDHTVKVILGSIAFAVFWLLAVFPAIPFLPIGRTAGSILGAMLMVVFRVLTPEQAYAA 60

Query: 61  IDLPILGLLFGTMVVSVYLERADMFKYLGKVLSWKSKGAKDLICRVCLISAISSAFFTND 120
           IDLPILGLLFGTMVVSVYLERADMFKYLGKVLSWKSKGAKDLICRVCLISAISSAFFTND
Sbjct: 61  IDLPILGLLFGTMVVSVYLERADMFKYLGKVLSWKSKGAKDLICRVCLISAISSAFFTND 120

Query: 121 TSCVVLTEFVLKIARQHNLPPRPFLLALASSANIGSSATPIGNPQNLVIAVQSKIPFGQF 180
           TSCVVLTEFVLKIARQHNLPPRPFLLALASSANIGSSATPIGNPQNLVIAVQSKI FGQF
Sbjct: 121 TSCVVLTEFVLKIARQHNLPPRPFLLALASSANIGSSATPIGNPQNLVIAVQSKIHFGQF 180

Query: 181 VIGILPAMLVGVVVNALIILIMYWKLLSVQKDEEDPSPEVIADEDVLSHRFSPARLSHSQ 240
           VIGILPAMLVGVVVNALIILIMYWKLLSVQKDEEDPSPEVIADEDVLSHRFSPARLSHSQ
Sbjct: 181 VIGILPAMLVGVVVNALIILIMYWKLLSVQKDEEDPSPEVIADEDVLSHRFSPARLSHSQ 240

Query: 241 IPSLNSAEWDSRLDLMNAQSPPCSNTNVETIRNSVSSKDNEEIRRSHSTMTEPARISDAS 300
           IPSLNSAEWDSRLDLMNAQSPPCSNTNVETIRNSVSSKDNEEIRRSHSTMTEPARISDAS
Sbjct: 241 IPSLNSAEWDSRLDLMNAQSPPCSNTNVETIRNSVSSKDNEEIRRSHSTMTEPARISDAS 300

Query: 301 KEWLPNASTQKREEDFSSKSLNSMEKQKEPVILQSSEGKEHWSTKWRRIAWKSCVYLVTV 360
           KEWLPNASTQKREEDFSSKSLNSMEKQKEPVILQSSEGKEHWSTKWRRIAWKSCVYLVTV
Sbjct: 301 KEWLPNASTQKREEDFSSKSLNSMEKQKEPVILQSSEGKEHWSTKWRRIAWKSCVYLVTV 360

Query: 361 GMLVALLMGLDMSWTAVTAALALVVLDFKDAQPCLEKVSYSLLVFFCGMFMTVDGFNKTG 420
           GMLVALLMGLDMSWTAVTAALALVVLDFKDAQPCLEKVSYSLLVFFCGMFMTVDGFNKTG
Sbjct: 361 GMLVALLMGLDMSWTAVTAALALVVLDFKDAQPCLEKVSYSLLVFFCGMFMTVDGFNKTG 420

Query: 421 LPSAFWNFMEPHAQIDRVSGTVVLALVILYLSNLASNVPTVLLLGARVAASAAAISPTEE 480
           LPSAFWNFMEPHAQIDRVSGTVVLALVILYLSNLASNVPTVLLLGARVAASAAAISPTEE
Sbjct: 421 LPSAFWNFMEPHAQIDRVSGTVVLALVILYLSNLASNVPTVLLLGARVAASAAAISPTEE 480

Query: 481 KRAWLLLAWISTVAGNLSLLGSAANLIVCEQARRTPQLSYNLSFWNHLKFGLPSTLIVTA 540
           KRAWLLLAWISTVAGNLSLLGSAANLIVCEQARRTPQLSYNLSFWNHLKFGLPSTLIVTA
Sbjct: 481 KRAWLLLAWISTVAGNLSLLGSAANLIVCEQARRTPQLSYNLSFWNHLKFGLPSTLIVTA 540

Query: 541 IGLVLIK 548
           IGLVLIK
Sbjct: 541 IGLVLIK 547

BLAST of Cucsa.107960 vs. TrEMBL
Match: F1SX52_CUCMO (Silicon efflux transporter CmLsi2-1 OS=Cucurbita moschata GN=CmLsi2-1 PE=2 SV=1)

HSP 1 Score: 961.8 bits (2485), Expect = 3.5e-277
Identity = 494/550 (89.82%), Postives = 517/550 (94.00%), Query Frame = 1

Query: 1   MAMDHTVKVILGSIAFAVFWLLAVFPAIPFLPIGRTAGSILGAMLMVVFRVLTPEQAYAA 60
           MAMDHTVKVILGSIAF +FWLLAVFPAIPFLPIGRTAGS+LGAMLMV+FRVLTP+QAYAA
Sbjct: 1   MAMDHTVKVILGSIAFGIFWLLAVFPAIPFLPIGRTAGSLLGAMLMVLFRVLTPDQAYAA 60

Query: 61  IDLPILGLLFGTMVVSVYLERADMFKYLGKVLSWKSKGAKDLICRVCLISAISSAFFTND 120
           IDLPILGLLFGTMVVSVYLERADMFKYLGKVLSWKSKGAKDLICRVCLISAISSA FTND
Sbjct: 61  IDLPILGLLFGTMVVSVYLERADMFKYLGKVLSWKSKGAKDLICRVCLISAISSALFTND 120

Query: 121 TSCVVLTEFVLKIARQHNLPPRPFLLALASSANIGSSATPIGNPQNLVIAVQSKIPFGQF 180
           TSCVVLTEFVLKIA+QHNLPPRPFLLALASSANIGSSATPIGNPQNLVIAVQS IPFGQF
Sbjct: 121 TSCVVLTEFVLKIAKQHNLPPRPFLLALASSANIGSSATPIGNPQNLVIAVQSGIPFGQF 180

Query: 181 VIGILPAMLVGVVVNALIILIMYWKLLSVQKDEEDPSPEVIADEDVLSHRFSPARLSHSQ 240
           VIGILPAMLVG+VVNALIILI YWKLLS+QKDEED  PE++ADEDV SHRFSPARLSHSQ
Sbjct: 181 VIGILPAMLVGIVVNALIILISYWKLLSIQKDEEDVPPEIVADEDVNSHRFSPARLSHSQ 240

Query: 241 IPSLNSAEWDSRLDLMNAQSPPCSNTNVETIRNSVSSKDNEEIRRSHSTMTEP-ARISDA 300
           IPSLNS EWDSRLD M AQSPP SN NVET+R S+SSKDN EI  SH  +TE  ARISDA
Sbjct: 241 IPSLNSQEWDSRLDSMYAQSPPNSNANVETLRTSLSSKDN-EIHWSHGAVTEAVARISDA 300

Query: 301 SKEWLPNAS--TQKREEDFSSKSLNSMEKQKEPVILQSSEGKEHWSTKWRRIAWKSCVYL 360
           SKE +PNAS   QKREE  SSKS NSM+KQKEP+ LQ SEGK+HWSTKWRR+AWK CVYL
Sbjct: 301 SKECVPNASPPPQKREEGLSSKSFNSMDKQKEPLPLQVSEGKQHWSTKWRRMAWKCCVYL 360

Query: 361 VTVGMLVALLMGLDMSWTAVTAALALVVLDFKDAQPCLEKVSYSLLVFFCGMFMTVDGFN 420
           VT+GMLVALLMGL+MSWTAVTAALALVVLDFKDAQPCLEKVSYSLLVFFCGMF+TVDGFN
Sbjct: 361 VTIGMLVALLMGLNMSWTAVTAALALVVLDFKDAQPCLEKVSYSLLVFFCGMFITVDGFN 420

Query: 421 KTGLPSAFWNFMEPHAQIDRVSGTVVLALVILYLSNLASNVPTVLLLGARVAASAAAISP 480
           KTGLP+ FWNFMEPHAQIDRVSGTVVLALVILYLSNLASNVPTVLLLGARVAASAAAISP
Sbjct: 421 KTGLPNTFWNFMEPHAQIDRVSGTVVLALVILYLSNLASNVPTVLLLGARVAASAAAISP 480

Query: 481 TEEKRAWLLLAWISTVAGNLSLLGSAANLIVCEQARRTPQLSYNLSFWNHLKFGLPSTLI 540
            +EKRAWLLLAWISTVAGNLSLLGSAANLIVCEQARRTPQLSYNLSFWNHLKFGLPSTLI
Sbjct: 481 AQEKRAWLLLAWISTVAGNLSLLGSAANLIVCEQARRTPQLSYNLSFWNHLKFGLPSTLI 540

Query: 541 VTAIGLVLIK 548
           +T IGLVLIK
Sbjct: 541 ITGIGLVLIK 549

BLAST of Cucsa.107960 vs. TrEMBL
Match: F1SX53_CUCMO (Silicon efflux transporter CmLsi2-2 OS=Cucurbita moschata GN=CmLsi2-2 PE=2 SV=1)

HSP 1 Score: 943.7 bits (2438), Expect = 9.8e-272
Identity = 493/547 (90.13%), Postives = 512/547 (93.60%), Query Frame = 1

Query: 1   MAMDHTVKVILGSIAFAVFWLLAVFPAIPFLPIGRTAGSILGAMLMVVFRVLTPEQAYAA 60
           MAMDHTVKVILGSIAFA FWLLAVFPAIPFLP+GRTAGS+LGAMLMVVFRVLTP+QAYAA
Sbjct: 1   MAMDHTVKVILGSIAFATFWLLAVFPAIPFLPVGRTAGSLLGAMLMVVFRVLTPDQAYAA 60

Query: 61  IDLPILGLLFGTMVVSVYLERADMFKYLGKVLSWKSKGAKDLICRVCLISAISSAFFTND 120
           IDLPILGLLFGTMVVSVYLERADMFKYLGKVLSW+SKGAKDLICRVCLISAISSAFFTND
Sbjct: 61  IDLPILGLLFGTMVVSVYLERADMFKYLGKVLSWRSKGAKDLICRVCLISAISSAFFTND 120

Query: 121 TSCVVLTEFVLKIARQHNLPPRPFLLALASSANIGSSATPIGNPQNLVIAVQSKIPFGQF 180
           TSCVVLTEFVLKIARQHNLPPRPFLLALASSANIGSSATPIGNPQNLVIAVQSKIPFG+F
Sbjct: 121 TSCVVLTEFVLKIARQHNLPPRPFLLALASSANIGSSATPIGNPQNLVIAVQSKIPFGEF 180

Query: 181 VIGILPAMLVGVVVNALIILIMYWKLLSVQKDEEDPSPEVIADEDVLSHRFSPARLSHSQ 240
           V+GILPAMLVGVVVNALIILI+YWKLLSVQKDEEDPS E++ADE VLSHRFSPARLSH+Q
Sbjct: 181 VVGILPAMLVGVVVNALIILILYWKLLSVQKDEEDPSSEIVADEVVLSHRFSPARLSHTQ 240

Query: 241 IPSLNSAEWDSRLDLMNAQSPPCSNTNVETIRNSVSSKDNEEIRRSHSTMTEPARISDAS 300
           IPSLNS EW+SRLD MNA        NVET R SVSSK+N EI RSHS +TE ARISD  
Sbjct: 241 IPSLNSPEWESRLDSMNA--------NVETQR-SVSSKEN-EIHRSHSALTESARISD-- 300

Query: 301 KEWLPNASTQKREEDFSSKSLNSMEKQKEPVILQSSEGKEHWSTKWRRIAWKSCVYLVTV 360
                 AS+ KREE FSSKSLNSM+KQKE V L SSE KEHWSTKWRRIAWK CVYLVT+
Sbjct: 301 ------ASSLKREEGFSSKSLNSMDKQKESVSLPSSEEKEHWSTKWRRIAWKCCVYLVTI 360

Query: 361 GMLVALLMGLDMSWTAVTAALALVVLDFKDAQPCLEKVSYSLLVFFCGMFMTVDGFNKTG 420
           GMLVALLMGL+MSWTAVTAALALVVLDFKDAQPCLEKVSYSLLVFFCGMFMTVDGFNKTG
Sbjct: 361 GMLVALLMGLNMSWTAVTAALALVVLDFKDAQPCLEKVSYSLLVFFCGMFMTVDGFNKTG 420

Query: 421 LPSAFWNFMEPHAQIDRVSGTVVLALVILYLSNLASNVPTVLLLGARVAASAAAISPTEE 480
           LPSAFW+FMEPHAQIDRVSGTVVLALVILYLSNLASNVPTVLLLGARVAASAAAISP EE
Sbjct: 421 LPSAFWDFMEPHAQIDRVSGTVVLALVILYLSNLASNVPTVLLLGARVAASAAAISPMEE 480

Query: 481 KRAWLLLAWISTVAGNLSLLGSAANLIVCEQARRTPQLSYNLSFWNHLKFGLPSTLIVTA 540
           KRAWLLLAWISTVAGNLSLLGSAANLIVCEQARRTPQLSYNLSFWNHLKFG+PSTLIVTA
Sbjct: 481 KRAWLLLAWISTVAGNLSLLGSAANLIVCEQARRTPQLSYNLSFWNHLKFGVPSTLIVTA 529

Query: 541 IGLVLIK 548
           IGLVLIK
Sbjct: 541 IGLVLIK 529

BLAST of Cucsa.107960 vs. TrEMBL
Match: M5X637_PRUPE (Uncharacterized protein OS=Prunus persica GN=PRUPE_ppa024761mg PE=4 SV=1)

HSP 1 Score: 800.8 bits (2067), Expect = 1.0e-228
Identity = 409/550 (74.36%), Postives = 474/550 (86.18%), Query Frame = 1

Query: 1   MAMDHTVKVILGSIAFAVFWLLAVFPAIPFLPIGRTAGSILGAMLMVVFRVLTPEQAYAA 60
           MAM  +VKV+LGSIAFAVFW+LAVFPA+PFLPIGRTAGS+LGAMLMV+FRVLTP+QAYAA
Sbjct: 1   MAMASSVKVVLGSIAFAVFWVLAVFPAVPFLPIGRTAGSLLGAMLMVIFRVLTPDQAYAA 60

Query: 61  IDLPILGLLFGTMVVSVYLERADMFKYLGKVLSWKSKGAKDLICRVCLISAISSAFFTND 120
           IDLPILGLLFGTMVVS+YLERADMFKYLGK+L WK++GAKDL+CR+CLISAISSA FTND
Sbjct: 61  IDLPILGLLFGTMVVSIYLERADMFKYLGKLLLWKTQGAKDLLCRICLISAISSALFTND 120

Query: 121 TSCVVLTEFVLKIARQHNLPPRPFLLALASSANIGSSATPIGNPQNLVIAVQSKIPFGQF 180
           TSCVVLTEFVLK+A+QHNLPP PFLLALASSANIGS+ATPIGNPQNLVIAVQSKIPFG+F
Sbjct: 121 TSCVVLTEFVLKVAKQHNLPPHPFLLALASSANIGSAATPIGNPQNLVIAVQSKIPFGKF 180

Query: 181 VIGILPAMLVGVVVNALIILIMYWKLLSVQKDEEDPSPEVIADEDVLSHRFSPARLSHSQ 240
           VIGILPAMLVG+ VNALI+L M+W+LLSV KDEEDP+ EV+ +EDV SHRFSPA LSHS 
Sbjct: 181 VIGILPAMLVGIFVNALILLCMFWRLLSVHKDEEDPAGEVVEEEDVSSHRFSPATLSHS- 240

Query: 241 IPSLNSAEWDSRLDLMNAQSPPCSN---TNVETIRNSVSSKDNEEIRRSHSTMTEPARIS 300
             SLNS EW+SRL+  + +S P  +    NVET+RN + S +N EI   HS   + AR S
Sbjct: 241 -ASLNSQEWNSRLETADVKSSPNFSRNIDNVETLRNRLPSSEN-EINNVHSGALDSARNS 300

Query: 301 DASKEWLPNASTQKREEDFSSKSLNSMEKQKEPVILQSSEGKEHWSTKWRRIAWKSCVYL 360
            ASKE + + S+ KREE   S+   S    ++    +S EGKE+ +T+W+R+ WKS VYL
Sbjct: 301 IASKEEIVDVSSHKREETILSERSASNNTLRDACPTESLEGKENLTTRWKRLLWKSGVYL 360

Query: 361 VTVGMLVALLMGLDMSWTAVTAALALVVLDFKDAQPCLEKVSYSLLVFFCGMFMTVDGFN 420
           VT+GML+ALLMGL+MSW A+TAALALVVLDF DA+P LEKVSYSLL+FFCGMF+TV+GFN
Sbjct: 361 VTIGMLIALLMGLNMSWCAITAALALVVLDFTDARPSLEKVSYSLLIFFCGMFITVEGFN 420

Query: 421 KTGLPSAFWNFMEPHAQIDRVSGTVVLALVILYLSNLASNVPTVLLLGARVAASAAAISP 480
           KTG+PSA W+FMEPHA IDR +G  VLAL IL LSNLASNVPTVLLLGARVAASAAAIS 
Sbjct: 421 KTGIPSALWDFMEPHAHIDRATGIAVLALTILVLSNLASNVPTVLLLGARVAASAAAISA 480

Query: 481 TEEKRAWLLLAWISTVAGNLSLLGSAANLIVCEQARRTPQLSYNLSFWNHLKFGLPSTLI 540
            EEK+AWLLLAW+STVAGNLSLLGSAANLIVCEQAR++P L+Y LSFW+HLKFG+PSTL+
Sbjct: 481 AEEKKAWLLLAWVSTVAGNLSLLGSAANLIVCEQARQSPYLAYTLSFWSHLKFGVPSTLL 540

Query: 541 VTAIGLVLIK 548
           VTAIGL LI+
Sbjct: 541 VTAIGLTLIR 547

BLAST of Cucsa.107960 vs. TrEMBL
Match: A0A067JQJ4_JATCU (Uncharacterized protein OS=Jatropha curcas GN=JCGZ_26081 PE=4 SV=1)

HSP 1 Score: 794.3 bits (2050), Expect = 9.6e-227
Identity = 403/551 (73.14%), Postives = 467/551 (84.75%), Query Frame = 1

Query: 1   MAMDHTVKVILGSIAFAVFWLLAVFPAIPFLPIGRTAGSILGAMLMVVFRVLTPEQAYAA 60
           M +  +VKV+LGSIAF++FW+LAVFP+IPFLP+GRTAGS+LGAMLMV+FRV+TP+QAYAA
Sbjct: 1   MVLASSVKVVLGSIAFSIFWILAVFPSIPFLPVGRTAGSLLGAMLMVIFRVMTPDQAYAA 60

Query: 61  IDLPILGLLFGTMVVSVYLERADMFKYLGKVLSWKSKGAKDLICRVCLISAISSAFFTND 120
           IDLPILGLLFGTMVVSVYLERADMFKYLG +LSWKSKGAKDL+CR+CL+SAISSA FTND
Sbjct: 61  IDLPILGLLFGTMVVSVYLERADMFKYLGNLLSWKSKGAKDLLCRICLVSAISSALFTND 120

Query: 121 TSCVVLTEFVLKIARQHNLPPRPFLLALASSANIGSSATPIGNPQNLVIAVQSKIPFGQF 180
           TSCVVLTEFVLKIARQHNLPP PFLLALASSANIGSSATPIGNPQNLVIA QSKI FG F
Sbjct: 121 TSCVVLTEFVLKIARQHNLPPHPFLLALASSANIGSSATPIGNPQNLVIAGQSKISFGDF 180

Query: 181 VIGILPAMLVGVVVNALIILIMYWKLLS-VQKDEEDPSPEVIADEDVLSHRFSPARLSHS 240
           + GILPAMLVG+VVN  I++ MYWKLLS  QKDEED + EV+ +EDV  HRFSPA LSH 
Sbjct: 181 LFGILPAMLVGIVVNIAILMCMYWKLLSSTQKDEEDATAEVVGEEDVNFHRFSPATLSH- 240

Query: 241 QIPSLNSAEWDSRLDLMNAQSPPCSN---TNVETIRNSVSSKDNEEIRRSHSTMTEPARI 300
              S NS EW+SRL+ +N QS P  N    + ET+RN ++S +NE I    ++  E AR 
Sbjct: 241 -FSSANSQEWNSRLESLNMQSSPNMNGHTNHAETLRNRITSNENE-IHSGTTSAYESARD 300

Query: 301 SDASKEWLPNASTQKREEDFSSKSLNSMEKQKEPVILQSSEGKEHWSTKWRRIAWKSCVY 360
           S+ASKE     S+QKREE   SK + SM+K ++ V+ + S  K  +STKW+R  WKS VY
Sbjct: 301 SNASKEETTAVSSQKREEHVPSKRIASMDKLRDVVVQEFSGEKPEFSTKWKRTLWKSSVY 360

Query: 361 LVTVGMLVALLMGLDMSWTAVTAALALVVLDFKDAQPCLEKVSYSLLVFFCGMFMTVDGF 420
           LVT+GML+ALLMGL+MSWTA+TAALALVVLDFKDA+PCLEKVSYSLL+FFCGMF+TVDGF
Sbjct: 361 LVTLGMLIALLMGLNMSWTAITAALALVVLDFKDARPCLEKVSYSLLIFFCGMFITVDGF 420

Query: 421 NKTGLPSAFWNFMEPHAQIDRVSGTVVLALVILYLSNLASNVPTVLLLGARVAASAAAIS 480
           NKTG+PSA W+ MEPHA ID  +GT VLA+VIL LSNLASNVPTVLLLG RVAASAAAIS
Sbjct: 421 NKTGIPSALWDLMEPHAHIDSAAGTAVLAVVILVLSNLASNVPTVLLLGGRVAASAAAIS 480

Query: 481 PTEEKRAWLLLAWISTVAGNLSLLGSAANLIVCEQARRTPQLSYNLSFWNHLKFGLPSTL 540
             +EK+AWL LAW+STVAGNLSLLGSAANLIVCEQARR PQ  YNL+FW HLKFG+PST+
Sbjct: 481 AADEKKAWLFLAWVSTVAGNLSLLGSAANLIVCEQARRAPQFGYNLTFWKHLKFGVPSTI 540

Query: 541 IVTAIGLVLIK 548
           +VTA+GL+LI+
Sbjct: 541 LVTAVGLMLIR 548

BLAST of Cucsa.107960 vs. TAIR10
Match: AT1G02260.1 (AT1G02260.1 Divalent ion symporter)

HSP 1 Score: 671.8 bits (1732), Expect = 3.6e-193
Identity = 354/551 (64.25%), Postives = 426/551 (77.31%), Query Frame = 1

Query: 1   MAMDHTVKVILGSIAFAVFWLLAVFPAIPFLPIGRTAGSILGAMLMVVFRVLTPEQAYAA 60
           MAM   +K++LGS+AFA+FW+LAVFP++PFLPIGRTAGS+ GAMLMV+F+V+TPEQAYAA
Sbjct: 1   MAMAPVIKLVLGSVAFAIFWILAVFPSVPFLPIGRTAGSLFGAMLMVIFQVITPEQAYAA 60

Query: 61  IDLPILGLLFGTMVVSVYLERADMFKYLGKVLSWKSKGAKDLICRVCLISAISSAFFTND 120
           IDLPILGLLFGTMVVS+YLERADMFKYLG +LSWKS+G KDL+CRVCL+SA+SSA FTND
Sbjct: 61  IDLPILGLLFGTMVVSIYLERADMFKYLGTLLSWKSRGPKDLLCRVCLVSAVSSALFTND 120

Query: 121 TSCVVLTEFVLKIARQHNLPPRPFLLALASSANIGSSATPIGNPQNLVIAVQSKIPFGQF 180
           TSCVVLTEFVLKIARQ NLPP PFLLALA+SANIGSSATPIGNPQNLVIAVQSKIPF +F
Sbjct: 121 TSCVVLTEFVLKIARQKNLPPHPFLLALATSANIGSSATPIGNPQNLVIAVQSKIPFWEF 180

Query: 181 VIGILPAMLVGVVVNALIILIMYWKLLSVQKDEED----PSPEVIADEDVLSHRFSPARL 240
           ++G+ PAM+VG+ VNA+++L MYW+LLS  K++E+       EV+A EDV SHRFSPA  
Sbjct: 181 LLGVFPAMIVGITVNAMLLLGMYWRLLSDHKEDEEEVQNADSEVVAQEDVQSHRFSPATF 240

Query: 241 SHSQIPSLNSAEWDSRLDLMNAQSPPCSNTNVETIRNSVSSKDNEEIRRSHSTMTEPARI 300
           S      ++S + + R+D              ET+RN   S    E+   +         
Sbjct: 241 S-----PVSSEDSNLRMDA------------AETLRNRAGSAGESELISCN--------- 300

Query: 301 SDASKEWLPNASTQKREEDFSSKSLNSMEKQKEPVILQSSEGKEHWSTKWRRIAWKSCVY 360
           S+AS+E   +A +Q       S + N+M + K                +WRR+ WKS VY
Sbjct: 301 SNASREQHNDAESQG-----ESNNTNNMFQTK----------------RWRRVLWKSSVY 360

Query: 361 LVTVGMLVALLMGLDMSWTAVTAALALVVLDFKDAQPCLEKVSYSLLVFFCGMFMTVDGF 420
            +T+GML++LLMGL+MSWTA+TAALALVVLDFKDA+P LEKVSYSLL+FFCGMF+TVDGF
Sbjct: 361 FITLGMLISLLMGLNMSWTAITAALALVVLDFKDARPSLEKVSYSLLIFFCGMFITVDGF 420

Query: 421 NKTGLPSAFWNFMEPHAQIDRVSGTVVLALVILYLSNLASNVPTVLLLGARVAASAAAIS 480
           NKTG+P+A W+ MEP+A+ID+  G  VLA+VIL LSN+ASNVPTVLLLGARVAAS  A+ 
Sbjct: 421 NKTGIPTALWDLMEPYAKIDQAKGIAVLAVVILVLSNVASNVPTVLLLGARVAAS--AMG 480

Query: 481 PTEEKRAWLLLAWISTVAGNLSLLGSAANLIVCEQARRTPQLSYNLSFWNHLKFGLPSTL 540
             EEK+AWLLLAW+STVAGNL+LLGSAANLIVCEQARR     Y L+F  H KFGLPSTL
Sbjct: 481 REEEKKAWLLLAWVSTVAGNLTLLGSAANLIVCEQARRAVSHGYTLTFTKHFKFGLPSTL 502

Query: 541 IVTAIGLVLIK 548
           IVTAIGL LIK
Sbjct: 541 IVTAIGLFLIK 502

BLAST of Cucsa.107960 vs. NCBI nr
Match: gi|449446019|ref|XP_004140769.1| (PREDICTED: putative transporter arsB [Cucumis sativus])

HSP 1 Score: 1067.0 bits (2758), Expect = 1.1e-308
Identity = 546/547 (99.82%), Postives = 546/547 (99.82%), Query Frame = 1

Query: 1   MAMDHTVKVILGSIAFAVFWLLAVFPAIPFLPIGRTAGSILGAMLMVVFRVLTPEQAYAA 60
           MAMDHTVKVILGSIAFAVFWLLAVFPAIPFLPIGRTAGSILGAMLMVVFRVLTPEQAYAA
Sbjct: 1   MAMDHTVKVILGSIAFAVFWLLAVFPAIPFLPIGRTAGSILGAMLMVVFRVLTPEQAYAA 60

Query: 61  IDLPILGLLFGTMVVSVYLERADMFKYLGKVLSWKSKGAKDLICRVCLISAISSAFFTND 120
           IDLPILGLLFGTMVVSVYLERADMFKYLGKVLSWKSKGAKDLICRVCLISAISSAFFTND
Sbjct: 61  IDLPILGLLFGTMVVSVYLERADMFKYLGKVLSWKSKGAKDLICRVCLISAISSAFFTND 120

Query: 121 TSCVVLTEFVLKIARQHNLPPRPFLLALASSANIGSSATPIGNPQNLVIAVQSKIPFGQF 180
           TSCVVLTEFVLKIARQHNLPPRPFLLALASSANIGSSATPIGNPQNLVIAVQSKI FGQF
Sbjct: 121 TSCVVLTEFVLKIARQHNLPPRPFLLALASSANIGSSATPIGNPQNLVIAVQSKIHFGQF 180

Query: 181 VIGILPAMLVGVVVNALIILIMYWKLLSVQKDEEDPSPEVIADEDVLSHRFSPARLSHSQ 240
           VIGILPAMLVGVVVNALIILIMYWKLLSVQKDEEDPSPEVIADEDVLSHRFSPARLSHSQ
Sbjct: 181 VIGILPAMLVGVVVNALIILIMYWKLLSVQKDEEDPSPEVIADEDVLSHRFSPARLSHSQ 240

Query: 241 IPSLNSAEWDSRLDLMNAQSPPCSNTNVETIRNSVSSKDNEEIRRSHSTMTEPARISDAS 300
           IPSLNSAEWDSRLDLMNAQSPPCSNTNVETIRNSVSSKDNEEIRRSHSTMTEPARISDAS
Sbjct: 241 IPSLNSAEWDSRLDLMNAQSPPCSNTNVETIRNSVSSKDNEEIRRSHSTMTEPARISDAS 300

Query: 301 KEWLPNASTQKREEDFSSKSLNSMEKQKEPVILQSSEGKEHWSTKWRRIAWKSCVYLVTV 360
           KEWLPNASTQKREEDFSSKSLNSMEKQKEPVILQSSEGKEHWSTKWRRIAWKSCVYLVTV
Sbjct: 301 KEWLPNASTQKREEDFSSKSLNSMEKQKEPVILQSSEGKEHWSTKWRRIAWKSCVYLVTV 360

Query: 361 GMLVALLMGLDMSWTAVTAALALVVLDFKDAQPCLEKVSYSLLVFFCGMFMTVDGFNKTG 420
           GMLVALLMGLDMSWTAVTAALALVVLDFKDAQPCLEKVSYSLLVFFCGMFMTVDGFNKTG
Sbjct: 361 GMLVALLMGLDMSWTAVTAALALVVLDFKDAQPCLEKVSYSLLVFFCGMFMTVDGFNKTG 420

Query: 421 LPSAFWNFMEPHAQIDRVSGTVVLALVILYLSNLASNVPTVLLLGARVAASAAAISPTEE 480
           LPSAFWNFMEPHAQIDRVSGTVVLALVILYLSNLASNVPTVLLLGARVAASAAAISPTEE
Sbjct: 421 LPSAFWNFMEPHAQIDRVSGTVVLALVILYLSNLASNVPTVLLLGARVAASAAAISPTEE 480

Query: 481 KRAWLLLAWISTVAGNLSLLGSAANLIVCEQARRTPQLSYNLSFWNHLKFGLPSTLIVTA 540
           KRAWLLLAWISTVAGNLSLLGSAANLIVCEQARRTPQLSYNLSFWNHLKFGLPSTLIVTA
Sbjct: 481 KRAWLLLAWISTVAGNLSLLGSAANLIVCEQARRTPQLSYNLSFWNHLKFGLPSTLIVTA 540

Query: 541 IGLVLIK 548
           IGLVLIK
Sbjct: 541 IGLVLIK 547

BLAST of Cucsa.107960 vs. NCBI nr
Match: gi|659077548|ref|XP_008439264.1| (PREDICTED: putative transporter arsB [Cucumis melo])

HSP 1 Score: 1044.3 bits (2699), Expect = 7.6e-302
Identity = 533/547 (97.44%), Postives = 540/547 (98.72%), Query Frame = 1

Query: 1   MAMDHTVKVILGSIAFAVFWLLAVFPAIPFLPIGRTAGSILGAMLMVVFRVLTPEQAYAA 60
           MAMDHTVKVILGSIAFAVFWLLAVFPAIPFLPIGRTAGSILGAMLMV+FRVLTPEQAYAA
Sbjct: 1   MAMDHTVKVILGSIAFAVFWLLAVFPAIPFLPIGRTAGSILGAMLMVIFRVLTPEQAYAA 60

Query: 61  IDLPILGLLFGTMVVSVYLERADMFKYLGKVLSWKSKGAKDLICRVCLISAISSAFFTND 120
           IDLPILGLLFGTMVVSVYLERADMFKYLGKVLSWKSKGAKDLICRVCLISAISSAFFTND
Sbjct: 61  IDLPILGLLFGTMVVSVYLERADMFKYLGKVLSWKSKGAKDLICRVCLISAISSAFFTND 120

Query: 121 TSCVVLTEFVLKIARQHNLPPRPFLLALASSANIGSSATPIGNPQNLVIAVQSKIPFGQF 180
           TSCVVLTEFVLKIARQHNLPPRPFLLALASSANIGSSATPIGNPQNLVIAVQSKIPFGQF
Sbjct: 121 TSCVVLTEFVLKIARQHNLPPRPFLLALASSANIGSSATPIGNPQNLVIAVQSKIPFGQF 180

Query: 181 VIGILPAMLVGVVVNALIILIMYWKLLSVQKDEEDPSPEVIADEDVLSHRFSPARLSHSQ 240
           VIGILPAMLVGVVVNALIILI+YWKLLSVQKDEEDPSPEVIADEDVLSHRFSPARLSHSQ
Sbjct: 181 VIGILPAMLVGVVVNALIILILYWKLLSVQKDEEDPSPEVIADEDVLSHRFSPARLSHSQ 240

Query: 241 IPSLNSAEWDSRLDLMNAQSPPCSNTNVETIRNSVSSKDNEEIRRSHSTMTEPARISDAS 300
           IPSLNSAEWDSRLD MNAQSPPCSN NVETIRNSVSSKDN EI RSHST+TEPARISDAS
Sbjct: 241 IPSLNSAEWDSRLDSMNAQSPPCSNANVETIRNSVSSKDN-EIHRSHSTLTEPARISDAS 300

Query: 301 KEWLPNASTQKREEDFSSKSLNSMEKQKEPVILQSSEGKEHWSTKWRRIAWKSCVYLVTV 360
           KEWLPNAS QKREE FSSKSLNSM+KQKEPVILQSSEGKEHWSTKWRRIAWKSCVYLVTV
Sbjct: 301 KEWLPNASPQKREEGFSSKSLNSMDKQKEPVILQSSEGKEHWSTKWRRIAWKSCVYLVTV 360

Query: 361 GMLVALLMGLDMSWTAVTAALALVVLDFKDAQPCLEKVSYSLLVFFCGMFMTVDGFNKTG 420
           GML+ALL+GLDMSWTAVTAALALVVLDFKDAQPCLEKVSYSLLVFFCGMFMTVDGFNKTG
Sbjct: 361 GMLIALLLGLDMSWTAVTAALALVVLDFKDAQPCLEKVSYSLLVFFCGMFMTVDGFNKTG 420

Query: 421 LPSAFWNFMEPHAQIDRVSGTVVLALVILYLSNLASNVPTVLLLGARVAASAAAISPTEE 480
           LPSAFWNFMEPHAQIDRVSGTVVLALVILYLSNLASNVPTVLLLGARVAASAAAISP EE
Sbjct: 421 LPSAFWNFMEPHAQIDRVSGTVVLALVILYLSNLASNVPTVLLLGARVAASAAAISPAEE 480

Query: 481 KRAWLLLAWISTVAGNLSLLGSAANLIVCEQARRTPQLSYNLSFWNHLKFGLPSTLIVTA 540
           KRAWLLLAWISTVAGNLSLLGSAANLIVCEQARRTPQLSYNLSFWNHLKFGLPSTLIVTA
Sbjct: 481 KRAWLLLAWISTVAGNLSLLGSAANLIVCEQARRTPQLSYNLSFWNHLKFGLPSTLIVTA 540

Query: 541 IGLVLIK 548
           IGLVLI+
Sbjct: 541 IGLVLIR 546

BLAST of Cucsa.107960 vs. NCBI nr
Match: gi|327187684|dbj|BAK09177.1| (silicon efflux transporter CmLsi2-1 [Cucurbita moschata])

HSP 1 Score: 961.8 bits (2485), Expect = 5.0e-277
Identity = 494/550 (89.82%), Postives = 517/550 (94.00%), Query Frame = 1

Query: 1   MAMDHTVKVILGSIAFAVFWLLAVFPAIPFLPIGRTAGSILGAMLMVVFRVLTPEQAYAA 60
           MAMDHTVKVILGSIAF +FWLLAVFPAIPFLPIGRTAGS+LGAMLMV+FRVLTP+QAYAA
Sbjct: 1   MAMDHTVKVILGSIAFGIFWLLAVFPAIPFLPIGRTAGSLLGAMLMVLFRVLTPDQAYAA 60

Query: 61  IDLPILGLLFGTMVVSVYLERADMFKYLGKVLSWKSKGAKDLICRVCLISAISSAFFTND 120
           IDLPILGLLFGTMVVSVYLERADMFKYLGKVLSWKSKGAKDLICRVCLISAISSA FTND
Sbjct: 61  IDLPILGLLFGTMVVSVYLERADMFKYLGKVLSWKSKGAKDLICRVCLISAISSALFTND 120

Query: 121 TSCVVLTEFVLKIARQHNLPPRPFLLALASSANIGSSATPIGNPQNLVIAVQSKIPFGQF 180
           TSCVVLTEFVLKIA+QHNLPPRPFLLALASSANIGSSATPIGNPQNLVIAVQS IPFGQF
Sbjct: 121 TSCVVLTEFVLKIAKQHNLPPRPFLLALASSANIGSSATPIGNPQNLVIAVQSGIPFGQF 180

Query: 181 VIGILPAMLVGVVVNALIILIMYWKLLSVQKDEEDPSPEVIADEDVLSHRFSPARLSHSQ 240
           VIGILPAMLVG+VVNALIILI YWKLLS+QKDEED  PE++ADEDV SHRFSPARLSHSQ
Sbjct: 181 VIGILPAMLVGIVVNALIILISYWKLLSIQKDEEDVPPEIVADEDVNSHRFSPARLSHSQ 240

Query: 241 IPSLNSAEWDSRLDLMNAQSPPCSNTNVETIRNSVSSKDNEEIRRSHSTMTEP-ARISDA 300
           IPSLNS EWDSRLD M AQSPP SN NVET+R S+SSKDN EI  SH  +TE  ARISDA
Sbjct: 241 IPSLNSQEWDSRLDSMYAQSPPNSNANVETLRTSLSSKDN-EIHWSHGAVTEAVARISDA 300

Query: 301 SKEWLPNAS--TQKREEDFSSKSLNSMEKQKEPVILQSSEGKEHWSTKWRRIAWKSCVYL 360
           SKE +PNAS   QKREE  SSKS NSM+KQKEP+ LQ SEGK+HWSTKWRR+AWK CVYL
Sbjct: 301 SKECVPNASPPPQKREEGLSSKSFNSMDKQKEPLPLQVSEGKQHWSTKWRRMAWKCCVYL 360

Query: 361 VTVGMLVALLMGLDMSWTAVTAALALVVLDFKDAQPCLEKVSYSLLVFFCGMFMTVDGFN 420
           VT+GMLVALLMGL+MSWTAVTAALALVVLDFKDAQPCLEKVSYSLLVFFCGMF+TVDGFN
Sbjct: 361 VTIGMLVALLMGLNMSWTAVTAALALVVLDFKDAQPCLEKVSYSLLVFFCGMFITVDGFN 420

Query: 421 KTGLPSAFWNFMEPHAQIDRVSGTVVLALVILYLSNLASNVPTVLLLGARVAASAAAISP 480
           KTGLP+ FWNFMEPHAQIDRVSGTVVLALVILYLSNLASNVPTVLLLGARVAASAAAISP
Sbjct: 421 KTGLPNTFWNFMEPHAQIDRVSGTVVLALVILYLSNLASNVPTVLLLGARVAASAAAISP 480

Query: 481 TEEKRAWLLLAWISTVAGNLSLLGSAANLIVCEQARRTPQLSYNLSFWNHLKFGLPSTLI 540
            +EKRAWLLLAWISTVAGNLSLLGSAANLIVCEQARRTPQLSYNLSFWNHLKFGLPSTLI
Sbjct: 481 AQEKRAWLLLAWISTVAGNLSLLGSAANLIVCEQARRTPQLSYNLSFWNHLKFGLPSTLI 540

Query: 541 VTAIGLVLIK 548
           +T IGLVLIK
Sbjct: 541 ITGIGLVLIK 549

BLAST of Cucsa.107960 vs. NCBI nr
Match: gi|327187686|dbj|BAK09178.1| (silicon efflux transporter CmLsi2-2 [Cucurbita moschata])

HSP 1 Score: 943.7 bits (2438), Expect = 1.4e-271
Identity = 493/547 (90.13%), Postives = 512/547 (93.60%), Query Frame = 1

Query: 1   MAMDHTVKVILGSIAFAVFWLLAVFPAIPFLPIGRTAGSILGAMLMVVFRVLTPEQAYAA 60
           MAMDHTVKVILGSIAFA FWLLAVFPAIPFLP+GRTAGS+LGAMLMVVFRVLTP+QAYAA
Sbjct: 1   MAMDHTVKVILGSIAFATFWLLAVFPAIPFLPVGRTAGSLLGAMLMVVFRVLTPDQAYAA 60

Query: 61  IDLPILGLLFGTMVVSVYLERADMFKYLGKVLSWKSKGAKDLICRVCLISAISSAFFTND 120
           IDLPILGLLFGTMVVSVYLERADMFKYLGKVLSW+SKGAKDLICRVCLISAISSAFFTND
Sbjct: 61  IDLPILGLLFGTMVVSVYLERADMFKYLGKVLSWRSKGAKDLICRVCLISAISSAFFTND 120

Query: 121 TSCVVLTEFVLKIARQHNLPPRPFLLALASSANIGSSATPIGNPQNLVIAVQSKIPFGQF 180
           TSCVVLTEFVLKIARQHNLPPRPFLLALASSANIGSSATPIGNPQNLVIAVQSKIPFG+F
Sbjct: 121 TSCVVLTEFVLKIARQHNLPPRPFLLALASSANIGSSATPIGNPQNLVIAVQSKIPFGEF 180

Query: 181 VIGILPAMLVGVVVNALIILIMYWKLLSVQKDEEDPSPEVIADEDVLSHRFSPARLSHSQ 240
           V+GILPAMLVGVVVNALIILI+YWKLLSVQKDEEDPS E++ADE VLSHRFSPARLSH+Q
Sbjct: 181 VVGILPAMLVGVVVNALIILILYWKLLSVQKDEEDPSSEIVADEVVLSHRFSPARLSHTQ 240

Query: 241 IPSLNSAEWDSRLDLMNAQSPPCSNTNVETIRNSVSSKDNEEIRRSHSTMTEPARISDAS 300
           IPSLNS EW+SRLD MNA        NVET R SVSSK+N EI RSHS +TE ARISD  
Sbjct: 241 IPSLNSPEWESRLDSMNA--------NVETQR-SVSSKEN-EIHRSHSALTESARISD-- 300

Query: 301 KEWLPNASTQKREEDFSSKSLNSMEKQKEPVILQSSEGKEHWSTKWRRIAWKSCVYLVTV 360
                 AS+ KREE FSSKSLNSM+KQKE V L SSE KEHWSTKWRRIAWK CVYLVT+
Sbjct: 301 ------ASSLKREEGFSSKSLNSMDKQKESVSLPSSEEKEHWSTKWRRIAWKCCVYLVTI 360

Query: 361 GMLVALLMGLDMSWTAVTAALALVVLDFKDAQPCLEKVSYSLLVFFCGMFMTVDGFNKTG 420
           GMLVALLMGL+MSWTAVTAALALVVLDFKDAQPCLEKVSYSLLVFFCGMFMTVDGFNKTG
Sbjct: 361 GMLVALLMGLNMSWTAVTAALALVVLDFKDAQPCLEKVSYSLLVFFCGMFMTVDGFNKTG 420

Query: 421 LPSAFWNFMEPHAQIDRVSGTVVLALVILYLSNLASNVPTVLLLGARVAASAAAISPTEE 480
           LPSAFW+FMEPHAQIDRVSGTVVLALVILYLSNLASNVPTVLLLGARVAASAAAISP EE
Sbjct: 421 LPSAFWDFMEPHAQIDRVSGTVVLALVILYLSNLASNVPTVLLLGARVAASAAAISPMEE 480

Query: 481 KRAWLLLAWISTVAGNLSLLGSAANLIVCEQARRTPQLSYNLSFWNHLKFGLPSTLIVTA 540
           KRAWLLLAWISTVAGNLSLLGSAANLIVCEQARRTPQLSYNLSFWNHLKFG+PSTLIVTA
Sbjct: 481 KRAWLLLAWISTVAGNLSLLGSAANLIVCEQARRTPQLSYNLSFWNHLKFGVPSTLIVTA 529

Query: 541 IGLVLIK 548
           IGLVLIK
Sbjct: 541 IGLVLIK 529

BLAST of Cucsa.107960 vs. NCBI nr
Match: gi|596047768|ref|XP_007220313.1| (hypothetical protein PRUPE_ppa024761mg [Prunus persica])

HSP 1 Score: 800.8 bits (2067), Expect = 1.5e-228
Identity = 409/550 (74.36%), Postives = 474/550 (86.18%), Query Frame = 1

Query: 1   MAMDHTVKVILGSIAFAVFWLLAVFPAIPFLPIGRTAGSILGAMLMVVFRVLTPEQAYAA 60
           MAM  +VKV+LGSIAFAVFW+LAVFPA+PFLPIGRTAGS+LGAMLMV+FRVLTP+QAYAA
Sbjct: 1   MAMASSVKVVLGSIAFAVFWVLAVFPAVPFLPIGRTAGSLLGAMLMVIFRVLTPDQAYAA 60

Query: 61  IDLPILGLLFGTMVVSVYLERADMFKYLGKVLSWKSKGAKDLICRVCLISAISSAFFTND 120
           IDLPILGLLFGTMVVS+YLERADMFKYLGK+L WK++GAKDL+CR+CLISAISSA FTND
Sbjct: 61  IDLPILGLLFGTMVVSIYLERADMFKYLGKLLLWKTQGAKDLLCRICLISAISSALFTND 120

Query: 121 TSCVVLTEFVLKIARQHNLPPRPFLLALASSANIGSSATPIGNPQNLVIAVQSKIPFGQF 180
           TSCVVLTEFVLK+A+QHNLPP PFLLALASSANIGS+ATPIGNPQNLVIAVQSKIPFG+F
Sbjct: 121 TSCVVLTEFVLKVAKQHNLPPHPFLLALASSANIGSAATPIGNPQNLVIAVQSKIPFGKF 180

Query: 181 VIGILPAMLVGVVVNALIILIMYWKLLSVQKDEEDPSPEVIADEDVLSHRFSPARLSHSQ 240
           VIGILPAMLVG+ VNALI+L M+W+LLSV KDEEDP+ EV+ +EDV SHRFSPA LSHS 
Sbjct: 181 VIGILPAMLVGIFVNALILLCMFWRLLSVHKDEEDPAGEVVEEEDVSSHRFSPATLSHS- 240

Query: 241 IPSLNSAEWDSRLDLMNAQSPPCSN---TNVETIRNSVSSKDNEEIRRSHSTMTEPARIS 300
             SLNS EW+SRL+  + +S P  +    NVET+RN + S +N EI   HS   + AR S
Sbjct: 241 -ASLNSQEWNSRLETADVKSSPNFSRNIDNVETLRNRLPSSEN-EINNVHSGALDSARNS 300

Query: 301 DASKEWLPNASTQKREEDFSSKSLNSMEKQKEPVILQSSEGKEHWSTKWRRIAWKSCVYL 360
            ASKE + + S+ KREE   S+   S    ++    +S EGKE+ +T+W+R+ WKS VYL
Sbjct: 301 IASKEEIVDVSSHKREETILSERSASNNTLRDACPTESLEGKENLTTRWKRLLWKSGVYL 360

Query: 361 VTVGMLVALLMGLDMSWTAVTAALALVVLDFKDAQPCLEKVSYSLLVFFCGMFMTVDGFN 420
           VT+GML+ALLMGL+MSW A+TAALALVVLDF DA+P LEKVSYSLL+FFCGMF+TV+GFN
Sbjct: 361 VTIGMLIALLMGLNMSWCAITAALALVVLDFTDARPSLEKVSYSLLIFFCGMFITVEGFN 420

Query: 421 KTGLPSAFWNFMEPHAQIDRVSGTVVLALVILYLSNLASNVPTVLLLGARVAASAAAISP 480
           KTG+PSA W+FMEPHA IDR +G  VLAL IL LSNLASNVPTVLLLGARVAASAAAIS 
Sbjct: 421 KTGIPSALWDFMEPHAHIDRATGIAVLALTILVLSNLASNVPTVLLLGARVAASAAAISA 480

Query: 481 TEEKRAWLLLAWISTVAGNLSLLGSAANLIVCEQARRTPQLSYNLSFWNHLKFGLPSTLI 540
            EEK+AWLLLAW+STVAGNLSLLGSAANLIVCEQAR++P L+Y LSFW+HLKFG+PSTL+
Sbjct: 481 AEEKKAWLLLAWVSTVAGNLSLLGSAANLIVCEQARQSPYLAYTLSFWSHLKFGVPSTLL 540

Query: 541 VTAIGLVLIK 548
           VTAIGL LI+
Sbjct: 541 VTAIGLTLIR 547