Lsi05G004730 (gene) Bottle gourd (USVL1VR-Ls) | Cucurbit Genomics Database (CuGenDB)

Overview


Name	Lsi05G004730
Type	gene
Organism	Lagenaria siceraria (Bottle gourd (USVL1VR-Ls))
Description	ATP synthase mitochondrial F1 complex assembly factor 2
Location	chr05 : 6054091 .. 6057994 (+)

Genome Viewer

Gene Expression

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: five_prime_UTRCDSthree_prime_UTR

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGCTTTCAAGTGTTGGAGTGCTTCTTGCTTCTTCTAAGAGCACCACACATTCCCTTTTTGATCCACTTGTAAATGTCATTGCTTCACCTTGTTCTTTTTCTTCTTCATTCAAAGTTTGTATGTTGTTGAGTAAGGTATCTTGTGGGTAATTTCCACTTCATTTGTACATTCTTCCATCTGGATCTCCAAAATTTGTTGAGATGTCGTTTCACTTTCAATGTTGACACCCAATGTAGATCTCGATCAAGTTGGATCGAAAAAAATTAGTCTAAGGAATTAAAAAAAAATATGCTAATTTTGAGAAGTGAAATTTAGAGGAAGGTACGTATAACCATTTCAGAGTATTATAGATTATTTCGTGTGAAATTTTGCAGACCATTTGTAAATCAACCCGTCCGAGTCCGAGTCGAGTCCGAAAACATGTAAACCGATCCGTGTATTTGCAATCCCTCTTCTTCCTCAATTTCTAAAACCCTAGCGCAGTTCACTCAGAAACACAAACATTAGGGGGAAAGCAAGAAAATGGCGAGCTCAATTTTATCAAAAATGGCGAAATCAATCAGAATCACCAATCCCACGCTAATTCCATTTCATTCATTCCGCTGCCGTATGCTTTCTACCAGTGTCGCCACCGCTGCCGAACAAGCTCAATCCGACGCTGCCTCCTTCACCTTCTCCGAGGACAACGCTAGAGAAGATCCTATTTTCGTCAAGGCTCCAGTTTCGAATTCCAGAGCCGATTCTTCATCCCCTTCCAGCTCCTCGGTGACGATGCCGACCTCGTTCATGACCGGTTCCGTCGTAGGCAAGCGATTTTACCAGCAAGTGACTACGAGGGAAGCGGATGATCGGAATGGATGGACGGTGATGCTTGATTACCGGACGCTGAAGACTCCTTCGAAGAGGCCGCTAAAGCTACCGACTCTCGGTCTCGCCAAGGCCGTTGCGGCTGAGTGGGAATATCAGGTGATTTCTCATTTCTTTTGATCGCGAAGTTTGAGTCCTCATCCTTTTTGGAAGGTTATTGAACTTTGTTTATCTGGATTGAAATGAAGCTTTGATCGATGTAGGAAACAGATGGGATTAGACCCTTCACAATGCCTCTAATGAAACTGGCCTGTACCGCATTGGAAAGAGTGCCTCTTACCCGGCCCAAGATTATCGAACATTTGATGAAGAAATTCAATCAGGATTTGGTTTTCTGTCGTGCTCCGGAGGACAATGTTCTAACAAGTGGAGTTTATGGTATGTCAAGTAATCCTATGAGCCCATAGCTAATTTCTTGATTATGACAGATTAGTACGTTTTGTTGTTCAAACTTTACTAGATGCATTGAGCATGAGCTTTACTTTAATTTCCATTCCCTAGCATTATTTTCTGTTGTGTCTAAAGGCTGAAGAATAACAGAATATGAACTATAGATTGAGTGGTATTTCGTTAATTTAAATTTATAACTCCTGCGTACAACATATTTAACTTAGAAGACCTCAAAAGTTGAGAGATCGGTTGAGTAGAACTTCCCAACATTTTTTTTTCTTTTTTTCTTTTTTGTTGTAGCTGCAATAGCCTTTACATCCTGATATGAGTGATCAACCACATTTCCTTTGGTAGATTTTTCCAACATTTTTGGTTATAACTGCAAGAACATAATACCCTGATTGAGTGATCAACAACATTTTTTTTTTTTTTTTTTTTTTTTTTTTNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGTAGCTGCAAATACATCTACATCTTGATATGAGTAATCGATGGCTCTTTTTAGTTGAAATTTTCAACATTTTTATTGTAGCTGCAATAACATCTAGATCCTGATATAAGTGAATTTAAATAAAGGGCAATAGAGGACGTTTAATTTTCTCGACACTATTTATTGAGCTTCATATCAGACTATCTGTCACAGTGTGATTCACATTTCAATAATGACTCATTCTGGTTTTGGCAGAAATTTTGAATGCCCATGCATGCAGCAACATAGCGAACATATTGCATGCAAACGTGGAATAGTAAATTAGATATACTGTATCTCCTTGACTTCTTCCGTTGGAAATGATGATACTCACTTGACAATTGCATGAACTCCTTTTTTGCAATGCGAAAACTTAAAGAATTAGAATTGAAAATTAATCAAGTTGTTTGATGAAAGAGATTATTATCTAGGAATGAATTTTCATAAAGGTTGAATACAAATTAACAGTAAAGGCTTCGGTATATAATAGGTGTTACATTTTCCTTGCAGAACGACAAGTGGAGAAAGTTGATCCTTTACTTGACTGGGTGCGCTCAGAATTTGGCTTTAAGCCTGTTGTATATTCCAGTTTTTTTGGTGGGAAGCAGGAGGACGGTCTTATAAAAGCTGTTGAAGATCTTCTAAGAAAAACAGATGACTGTGAATTGGCATCAATCGATGCCATTGCGTCAGCTGCACACTCTTTAATAATTGCTATTGGAATTTTTCGTGGAAAGTTGCAGATCGAAGAAGCAATTGAGTTGATTAGACTCGAAGAAGATCTGCAGGTTTTACTTCTCTCTCTTATTCTTATTCTTTATCATGCATAAATTTATTCTGCGTTTCCAGAGTCTAGGATGATGTCATATTAAGAGGCAAATGTCATTAACATATTCTAAACCAAAAAGAACCTGCTCGACCATTTACAGGTGGATAAATGGGGTCTGGTTGAAGGTGGTCACGATGTCGATATTGCTGATCTACGGGTTCAGATCTCATCTGCCACGGTATTTCTCGCTCTTTCAAGGAAATTTTGAAACCATTACTTTCTTATTGTACCCTGAGAGCTAGGATGGTGTCTTTTTTTTAGTTTTTAGTTTTTTGTATCTTGTTGTAGATCCAAAGGGCCTTTCTAAATTATGTTCTAAGACTGTTTATAGGCATACAATAGCTATGAGCAGTTGAGAAAATAATTATATTTACTGGAAAATAATGGATAGATTATTTTTGTAAACATTTCCCTCTGGCCAAGAAAGAACTTAGATGCCACTGTCTTTAATCTTTGGCTTTGTGAGAGTAAATCAAAATCACATACAAAATAATGCATAGAAGAAGGCTTTTAGAAGAGTTTATATTCAATCCAAGTGAAGTAATAGCTGAGCATGTTTAGTAGTGTAGACATTGTCAAAGCTCTTGGCAGCATTGCACACAGAGAGGTGGCTGTTTCCAGATATCACAGTTACAACAGGCAGTGTCACAGTCATTATTGCACCCTACATTAGACATTTTCCAATATGAGAGTTCTTTTGGTTAAGATTTCATAAATGTTAACAAGGATATGCTTTTTGCAAGTGCTAGGTGAATTTGGCTATCTCAATTCTTGGTAAGTCAAGATTCTCATCTATGCTCTTCTTTTAATTTACTACCCATTGTTTCTATGTTTAATGAGAATTGACAAATGGTTTCATAAGAACAAAAAGAAGTTTTTGTTTTTTAAAAGATACCATTTCTTTTAGTACAAAAGTCCTGATCATAGTTATTATTAAGAGTTTGTTGACATCAAATGTCTAATCTCAAATTTATGAAAAATGTTCATGTCATTGATATTGTGAAGGAAACCTTAAAGTCAAATTCATGTAAATTTATCAATATGTTGTAGATTATGGCCGTCG

mRNA sequence

ATGCTTTCAAGTGTTGGAGTGCTTCTTGCTTCTTCTAAGAGCACCACACATTCCCTTTTTGATCCACTTGTAAATGTCATTGCTTCACCTTGTTCTTTTTCTTCTTCATTCAAAGTTTGTATGTTGTTGAGTAAGGTATCTTGTGGGTAATTTCCACTTCATTTGTACATTCTTCCATCTGGATCTCCAAAATTTGTTGAGATGTCGTTTCACTTTCAATGTTGACACCCAATGTAGATCTCGATCAAGTTGGATCGAAAAAAATTAGTCTAAGGAATTAAAAAAAAATATGCTAATTTTGAGAAGTGAAATTTAGAGGAAGGTACGTATAACCATTTCAGAGTATTATAGATTATTTCGTGTGAAATTTTGCAGACCATTTGTAAATCAACCCGTCCGAGTCCGAGTCGAGTCCGAAAACATGTAAACCGATCCGTGTATTTGCAATCCCTCTTCTTCCTCAATTTCTAAAACCCTAGCGCAGTTCACTCAGAAACACAAACATTAGGGGGAAAGCAAGAAAATGGCGAGCTCAATTTTATCAAAAATGGCGAAATCAATCAGAATCACCAATCCCACGCTAATTCCATTTCATTCATTCCGCTGCCGTATGCTTTCTACCAGTGTCGCCACCGCTGCCGAACAAGCTCAATCCGACGCTGCCTCCTTCACCTTCTCCGAGGACAACGCTAGAGAAGATCCTATTTTCGTCAAGGCTCCAGTTTCGAATTCCAGAGCCGATTCTTCATCCCCTTCCAGCTCCTCGGTGACGATGCCGACCTCGTTCATGACCGGTTCCGTCGTAGGCAAGCGATTTTACCAGCAAGTGACTACGAGGGAAGCGGATGATCGGAATGGATGGACGGTGATGCTTGATTACCGGACGCTGAAGACTCCTTCGAAGAGGCCGCTAAAGCTACCGACTCTCGGTCTCGCCAAGGCCGTTGCGGCTGAGTGGGAATATCAGGAAACAGATGGGATTAGACCCTTCACAATGCCTCTAATGAAACTGGCCTGTACCGCATTGGAAAGAGTGCCTCTTACCCGGCCCAAGATTATCGAACATTTGATGAAGAAATTCAATCAGGATTTGGTTTTCTGTCGTGCTCCGGAGGACAATGTTCTAACAAGTGGAGTTTATGAACGACAAGTGGAGAAAGTTGATCCTTTACTTGACTGGGTGCGCTCAGAATTTGGCTTTAAGCCTGTTGTATATTCCAGTTTTTTTGGTGGGAAGCAGGAGGACGGTCTTATAAAAGCTGTTGAAGATCTTCTAAGAAAAACAGATGACTGTGAATTGGCATCAATCGATGCCATTGCGTCAGCTGCACACTCTTTAATAATTGCTATTGGAATTTTTCGTGGAAAGTTGCAGATCGAAGAAGCAATTGAGTTGATTAGACTCGAAGAAGATCTGCAGGTGGATAAATGGGGTCTGGTTGAAGGTGGTCACGATGTCGATATTGCTGATCTACGGGTTCAGATCTCATCTGCCACGGTATTTCTCGCTCTTTCAAGGAAATTTTGAAACCATTACTTTCTTATTGTACCCTGAGAGCTAGGATGGTGTCTTTTTTTTAGTTTTTAGTTTTTTGTATCTTGTTGTAGATCCAAAGGGCCTTTCTAAATTATGTTCTAAGACTGTTTATAGGCATACAATAGCTATGAGCAGTTGAGAAAATAATTATATTTACTGGAAAATAATGGATAGATTATTTTTGTAAACATTTCCCTCTGGCCAAGAAAGAACTTAGATGCCACTGTCTTTAATCTTTGGCTTTGTGAGAGTAAATCAAAATCACATACAAAATAATGCATAGAAGAAGGCTTTTAGAAGAGTTTATATTCAATCCAAGTGAAGTAATAGCTGAGCATGTTTAGTAGTGTAGACATTGTCAAAGCTCTTGGCAGCATTGCACACAGAGAGGTGGCTGTTTCCAGATATCACAGTTACAACAGGCAGTGTCACAGTCATTATTGCACCCTACATTAGACATTTTCCAATATGAGAGTTCTTTTGGTTAAGATTTCATAAATGTTAACAAGGATATGCTTTTTGCAAGTGCTAGGTGAATTTGGCTATCTCAATTCTTGGTAAGTCAAGATTCTCATCTATGCTCTTCTTTTAATTTACTACCCATTGTTTCTATGTTTAATGAGAATTGACAAATGGTTTCATAAGAACAAAAAGAAGTTTTTGTTTTTTAAAAGATACCATTTCTTTTAGTACAAAAGTCCTGATCATAGTTATTATTAAGAGTTTGTTGACATCAAATGTCTAATCTCAAATTTATGAAAAATGTTCATGTCATTGATATTGTGAAGGAAACCTTAAAGTCAAATTCATGTAAATTTATCAATATGTTGTAGATTATGGCCGTCG

Coding sequence (CDS)

ATGGCGAGCTCAATTTTATCAAAAATGGCGAAATCAATCAGAATCACCAATCCCACGCTAATTCCATTTCATTCATTCCGCTGCCGTATGCTTTCTACCAGTGTCGCCACCGCTGCCGAACAAGCTCAATCCGACGCTGCCTCCTTCACCTTCTCCGAGGACAACGCTAGAGAAGATCCTATTTTCGTCAAGGCTCCAGTTTCGAATTCCAGAGCCGATTCTTCATCCCCTTCCAGCTCCTCGGTGACGATGCCGACCTCGTTCATGACCGGTTCCGTCGTAGGCAAGCGATTTTACCAGCAAGTGACTACGAGGGAAGCGGATGATCGGAATGGATGGACGGTGATGCTTGATTACCGGACGCTGAAGACTCCTTCGAAGAGGCCGCTAAAGCTACCGACTCTCGGTCTCGCCAAGGCCGTTGCGGCTGAGTGGGAATATCAGGAAACAGATGGGATTAGACCCTTCACAATGCCTCTAATGAAACTGGCCTGTACCGCATTGGAAAGAGTGCCTCTTACCCGGCCCAAGATTATCGAACATTTGATGAAGAAATTCAATCAGGATTTGGTTTTCTGTCGTGCTCCGGAGGACAATGTTCTAACAAGTGGAGTTTATGAACGACAAGTGGAGAAAGTTGATCCTTTACTTGACTGGGTGCGCTCAGAATTTGGCTTTAAGCCTGTTGTATATTCCAGTTTTTTTGGTGGGAAGCAGGAGGACGGTCTTATAAAAGCTGTTGAAGATCTTCTAAGAAAAACAGATGACTGTGAATTGGCATCAATCGATGCCATTGCGTCAGCTGCACACTCTTTAATAATTGCTATTGGAATTTTTCGTGGAAAGTTGCAGATCGAAGAAGCAATTGAGTTGATTAGACTCGAAGAAGATCTGCAGGTGGATAAATGGGGTCTGGTTGAAGGTGGTCACGATGTCGATATTGCTGATCTACGGGTTCAGATCTCATCTGCCACGGTATTTCTCGCTCTTTCAAGGAAATTTTGA

Protein sequence

MASSILSKMAKSIRITNPTLIPFHSFRCRMLSTSVATAAEQAQSDAASFTFSEDNAREDPIFVKAPVSNSRADSSSPSSSSVTMPTSFMTGSVVGKRFYQQVTTREADDRNGWTVMLDYRTLKTPSKRPLKLPTLGLAKAVAAEWEYQETDGIRPFTMPLMKLACTALERVPLTRPKIIEHLMKKFNQDLVFCRAPEDNVLTSGVYERQVEKVDPLLDWVRSEFGFKPVVYSSFFGGKQEDGLIKAVEDLLRKTDDCELASIDAIASAAHSLIIAIGIFRGKLQIEEAIELIRLEEDLQVDKWGLVEGGHDVDIADLRVQISSATVFLALSRKF

Homology

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BLAST of Lsi05G004730 vs. Swiss-Prot
Match: ATPF2_HUMAN (ATP synthase mitochondrial F1 complex assembly factor 2 OS=Homo sapiens GN=ATPAF2 PE=1 SV=1)

HSP 1 Score: 120.9 bits (302), Expect = 2.6e-26
Identity = 74/237 (31.22%), Postives = 126/237 (53.16%), Query Frame = 1


Query: 96  KRFYQQVTTREADDRNGWTVMLDYRTLKTPSKRPLKLPTLGLAKAVAAEWEYQETDGIRP 155
           KRFYQ V+  + +   G+ + LD+R LKTP  +   +P+  LA AVA EW+ Q+ D I+ 
Sbjct: 47  KRFYQNVSITQGE--GGFEINLDHRKLKTPQAKLFTVPSEALAIAVATEWDSQQ-DTIKY 106

Query: 156 FTMPLMKLACTALERVPLTRPK--IIEHLMKKFNQDLVFCRAPEDNVLTSGVYERQVEKV 215
           +TM L  L  T+L+  P  R K  +I   +K  + D +  R  E   L     E Q  + 
Sbjct: 107 YTMHLTTLCNTSLDN-PTQRNKDQLIRAAVKFLDTDTICYRVEEPETLV----ELQRNEW 166

Query: 216 DPLLDWVRSEFGFKPVVYSSFFGGKQEDGLIKAVEDLLRKTDDCELASIDAIASAAHSLI 275
           DP+++W    +G +    +S  G        + +   L   +   L  I+ +A+   S++
Sbjct: 167 DPIIEWAEKRYGVEISSSTSIMGPSIPAKTREVLVSHLASYNTWALQGIEFVAAQLKSMV 226

Query: 276 IAIGIFRGKLQIEEAIELIRLEEDLQVDKWGLVEGGHDVDIADLRVQISSATVFLAL 331
           + +G+   +L +E+A+ L RLEE+ Q+ KWG +E  HD ++ +LR + ++ T+F+ L
Sbjct: 227 LTLGLIDLRLTVEQAVLLSRLEEEYQIQKWGNIEWAHDYELQELRARTAAGTLFIHL 275

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BLAST of Lsi05G004730 vs. Swiss-Prot
Match: ATPF2_MOUSE (ATP synthase mitochondrial F1 complex assembly factor 2 OS=Mus musculus GN=Atpaf2 PE=1 SV=1)

HSP 1 Score: 118.6 bits (296), Expect = 1.3e-25
Identity = 73/237 (30.80%), Postives = 125/237 (52.74%), Query Frame = 1


Query: 96  KRFYQQVTTREADDRNGWTVMLDYRTLKTPSKRPLKLPTLGLAKAVAAEWEYQETDGIRP 155
           KRFYQ V+  + +   G+ + LD+R LKTP  +   +P+  LA AVA EW+ Q+ D I+ 
Sbjct: 47  KRFYQNVSISQGE--GGFEINLDHRKLKTPQAKLFTVPSEALAIAVATEWDSQQ-DTIKF 106

Query: 156 FTMPLMKLACTALERVPLTRPK--IIEHLMKKFNQDLVFCRAPEDNVLTSGVYERQVEKV 215
           +TM L  L  T+L+  P  R K  +I   +K  + D +  R  E   L     E Q  + 
Sbjct: 107 YTMHLTTLCNTSLDN-PTQRSKDQLIRAAVKFLDTDTICYRVEEPETLV----ELQKNEW 166

Query: 216 DPLLDWVRSEFGFKPVVYSSFFGGKQEDGLIKAVEDLLRKTDDCELASIDAIASAAHSLI 275
           DP+++W    +G +    +S  G        + +   L   +   L  I+ + +   S++
Sbjct: 167 DPVIEWAEKRYGMEIGSSTSIMGPSIPTQTREVLTSHLSSYNMWALQGIEFVVAQLKSML 226

Query: 276 IAIGIFRGKLQIEEAIELIRLEEDLQVDKWGLVEGGHDVDIADLRVQISSATVFLAL 331
           + +G+   +L +E+A+ L RLEE+ Q+ KWG +E  HD ++ +LR + ++ T+F+ L
Sbjct: 227 LTLGLIDLRLTVEQAVLLSRLEEEYQIQKWGNIEWAHDYELQELRARTAAGTLFVHL 275

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BLAST of Lsi05G004730 vs. Swiss-Prot
Match: ATPF2_BOVIN (ATP synthase mitochondrial F1 complex assembly factor 2 OS=Bos taurus GN=ATPAF2 PE=2 SV=1)

HSP 1 Score: 115.5 bits (288), Expect = 1.1e-24
Identity = 74/237 (31.22%), Postives = 124/237 (52.32%), Query Frame = 1


Query: 96  KRFYQQVTTREADDRNGWTVMLDYRTLKTPSKRPLKLPTLGLAKAVAAEWEYQETDGIRP 155
           KRFYQ V+  + +   G+ + LD+R L+TP  +   +P+  LA AVA EW+ Q+ D I+ 
Sbjct: 47  KRFYQNVSISQGE--GGFEINLDHRKLRTPQGKLFTVPSEALAIAVATEWDSQQ-DTIKM 106

Query: 156 FTMPLMKLACTALERVPLTRPK--IIEHLMKKFNQDLVFCRAPEDNVLTSGVYERQVEKV 215
           +TM L  L  T+L+  P  R K  +I   +K  + D V  R  E   L     E Q  + 
Sbjct: 107 YTMHLTTLCNTSLDN-PTQRDKDQLIRAAVKFLDTDTVCYRVEEPETLV----ELQRNEW 166

Query: 216 DPLLDWVRSEFGFKPVVYSSFFGGKQEDGLIKAVEDLLRKTDDCELASIDAIASAAHSLI 275
           DP++ W    +G +    +S  G        + +   L   +   L  I+ + +   SL+
Sbjct: 167 DPVISWAEKRYGVEIGSSTSITGPSIPARTREVLVSHLASYNMWALQGIEFVVTQLKSLV 226

Query: 276 IAIGIFRGKLQIEEAIELIRLEEDLQVDKWGLVEGGHDVDIADLRVQISSATVFLAL 331
           + +G+   +L +E+A+ L RLEE+ Q+ KWG +E  HD ++ +LR + ++ T+F+ L
Sbjct: 227 LTLGLTDLRLTVEQAVLLSRLEEEYQIQKWGNIEWAHDYELQELRARTAAGTLFVHL 275

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BLAST of Lsi05G004730 vs. Swiss-Prot
Match: ATP12_SCHPO (Protein atp12, mitochondrial OS=Schizosaccharomyces pombe (strain 972 / ATCC 24843) GN=atp12 PE=3 SV=2)

HSP 1 Score: 79.7 bits (195), Expect = 6.6e-14
Identity = 61/244 (25.00%), Postives = 114/244 (46.72%), Query Frame = 1


Query: 96  KRFYQQVTTREADDRNGWT-VMLDYRTLKTPSKRPLKLPTLG--LAKAVAAEWEYQETDG 155
           +RF++   T+    +NG   + LD R LK+PS + +K+P     LA  +A EW+   +  
Sbjct: 39  RRFWKNTATKI---QNGEVLIQLDGRNLKSPSGKIVKVPKEMELLAHLIALEWDRLPSTS 98

Query: 156 IRPFTMPLMKLACTALERVPLTRPK---IIEHLMKKFNQDLVFCRAPEDNVLTSGVYERQ 215
           +R   +P+  L   A++     + +   +   L++  + D +   +PE       + E Q
Sbjct: 99  VRQHNLPITSLVSRAIDISQFKKEEKELLSTQLIRFLDTDTILIYSPETEY-EGKLLEEQ 158

Query: 216 VEKVDPLLDWVRSEFGFKPVVYSSFFGG-------KQEDGLIKAVEDLLRKTDDCELASI 275
            E   PL +   ++ G    V  S+  G       KQ     + + + L   +  +LA+ 
Sbjct: 159 KENWWPLKETFENKLG----VQLSYLDGDAGIIAHKQTQETHERIRNWLSSLNSWQLAAF 218

Query: 276 DAIASAAHSLIIAIGIFRGKLQIEEAIELIRLEEDLQVDKWGLVEGGHDVDIADLRVQIS 327
           +   S   S I++  I +G L  E+A  L  LE   Q ++WG +E  H++D  DL+ +++
Sbjct: 219 ERSVSCCKSFIVSFMILKGYLNSEKAAALTNLELQYQTNRWGSLEDAHEIDNEDLKNKLA 274

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BLAST of Lsi05G004730 vs. Swiss-Prot
Match: ATP12_YEAST (Protein ATP12, mitochondrial OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) GN=ATP12 PE=1 SV=2)

HSP 1 Score: 53.1 bits (126), Expect = 6.6e-06
Identity = 60/280 (21.43%), Postives = 108/280 (38.57%), Query Frame = 1


Query: 85  PTSFMTGSVVGKRFYQQVTTREADDRNGWTVMLDYRTLKTPSKRPLKLPTLG--LAKAVA 144
           PT     S   ++F+++V+     ++    + LD RT+KTP    + +      LA  + 
Sbjct: 41  PTETNRLSKTSQKFWEKVSLNRDVEKGKIALQLDGRTIKTPLGNGIIVDNAKSLLAYLLK 100

Query: 145 AEWEYQETDGIRPFTMPLMKLACTALERVPLTRPKIIEHLMKKF--NQD----------- 204
            EW    +  I+  ++PL  L    ++      P     L+ K   N D           
Sbjct: 101 LEWSSLSSLSIKTHSLPLTSLVARCIDLQMTNEPGCDPQLVAKIGGNSDVIKNQLLRYLD 160

Query: 205 ----LVFCRAPE-DNVLTSGVYERQVEKVDPLLDWVR---SEFGFKPVVYSSFF----GG 264
               LVF    E +  L +   E  +  +  + +++R   SE   +  +  +      G 
Sbjct: 161 TDTLLVFSPMNEFEGRLRNAQNELYIPIIKGMEEFLRNFSSESNIRLQILDADIHGLRGN 220

Query: 265 KQEDGLIKAVEDLLRKTDDCELASIDAIASAAHSLIIAIGIFRGKLQIEEAIELIRLEED 324
           +Q D +  A +  +      +LA ++       S I  + +   K      I  ++ + D
Sbjct: 221 QQSDIVKNAAKKYMSSLSPWDLAILEKTVLTTKSFICGVLLLENKKDTANLIPALKTDMD 280

Query: 325 -----------LQVDKWGLVEGGHDVDIADLRVQISSATV 327
                       QV+KWG VE  HDVD  D+R +I +A +
Sbjct: 281 NIVRAATLETIFQVEKWGEVEDTHDVDKRDIRRKIHTAAI 320

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BLAST of Lsi05G004730 vs. TrEMBL
Match: E5GCD8_CUCME (ATP12-like protein OS=Cucumis melo subsp. melo PE=4 SV=1)

HSP 1 Score: 575.9 bits (1483), Expect = 3.3e-161
Identity = 297/334 (88.92%), Postives = 314/334 (94.01%), Query Frame = 1


Query: 1   MASSILSKMAKSIRITNPTLIPFHSFRCRMLSTSVATAAEQAQSDAASFTFSEDNAREDP 60
           M++SILS MAKSIRITNPTL P HSFR RML TSVATA EQ+ SDAASFTFS DN RE+P
Sbjct: 1   MSTSILSIMAKSIRITNPTLTPSHSFRYRMLCTSVATAPEQSPSDAASFTFSNDNPREEP 60

Query: 61  IFVKAPVSNSRADSSSPSSSSVTMPTSFMTGSVVGKRFYQQVTTREADDRNGWTVMLDYR 120
           +F+KAP    R++SSSPSSSSVTMPTSFMTGS+VGKRFYQ+VTTREADD NGWTVMLDYR
Sbjct: 61  VFIKAP----RSNSSSPSSSSVTMPTSFMTGSIVGKRFYQKVTTREADDGNGWTVMLDYR 120

Query: 121 TLKTPSKRPLKLPTLGLAKAVAAEWEYQETDGIRPFTMPLMKLACTALERVPLTRPKIIE 180
           TLKTP+KRPLKLPTLGLAKAVAAEWEYQETDGIRPFTMPLMKLACTALERVPLTRPKIIE
Sbjct: 121 TLKTPTKRPLKLPTLGLAKAVAAEWEYQETDGIRPFTMPLMKLACTALERVPLTRPKIIE 180

Query: 181 HLMKKFNQDLVFCRAPEDNVLTSGVYERQVEKVDPLLDWVRSEFGFKPVVYSSFFGGKQE 240
           HLM KFN+DLVFCRAPED+ LTSGVYERQVEK+DPLLDWV SEFGFKP+VYSS FGG QE
Sbjct: 181 HLMGKFNRDLVFCRAPEDD-LTSGVYERQVEKIDPLLDWVHSEFGFKPIVYSSLFGGNQE 240

Query: 241 DGLIKAVEDLLRKTDDCELASIDAIASAAHSLIIAIGIFRGKLQIEEAIELIRLEEDLQV 300
           DGLIKAVEDLLRKTDDCELASIDAIASAAHSLIIAIGIFRGKLQIEEAIELIRLEED QV
Sbjct: 241 DGLIKAVEDLLRKTDDCELASIDAIASAAHSLIIAIGIFRGKLQIEEAIELIRLEEDFQV 300

Query: 301 DKWGLVEGGHDVDIADLRVQISSATVFLALSRKF 335
           DKWGLVEGGHDVDIADL+VQISSATVFLALSR+F
Sbjct: 301 DKWGLVEGGHDVDIADLQVQISSATVFLALSRRF 329

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BLAST of Lsi05G004730 vs. TrEMBL
Match: A0A0A0L8R2_CUCSA (Uncharacterized protein OS=Cucumis sativus GN=Csa_3G595240 PE=4 SV=1)

HSP 1 Score: 572.0 bits (1473), Expect = 4.7e-160
Identity = 295/333 (88.59%), Postives = 311/333 (93.39%), Query Frame = 1


Query: 1   MASSILSKMAKSIRITNPTLIPFHSFRCRMLSTSVATAAEQAQSDAASFTFSEDNAREDP 60
           M+SSILSKMAKSIRITNPTL P HSFR RML TSVATAAEQ+  D  SFTFS DN R++P
Sbjct: 1   MSSSILSKMAKSIRITNPTLSPSHSFRYRMLCTSVATAAEQSPLDDTSFTFSNDNPRDEP 60

Query: 61  IFVKAPVSNSRADSSSPSSSSVTMPTSFMTGSVVGKRFYQQVTTREADDRNGWTVMLDYR 120
           +FVKAP SNS    SS SSSSVTMPTSFMTGS+VGKRFYQ+VTTREADDRNGW VMLDYR
Sbjct: 61  VFVKAPRSNS----SSRSSSSVTMPTSFMTGSIVGKRFYQKVTTREADDRNGWAVMLDYR 120

Query: 121 TLKTPSKRPLKLPTLGLAKAVAAEWEYQETDGIRPFTMPLMKLACTALERVPLTRPKIIE 180
           TLKTP+KRPLKLPTLGLAKAVAAEWEYQETDGIRPFTMPLMKLACTALERVPLTR  IIE
Sbjct: 121 TLKTPTKRPLKLPTLGLAKAVAAEWEYQETDGIRPFTMPLMKLACTALERVPLTRHTIIE 180

Query: 181 HLMKKFNQDLVFCRAPEDNVLTSGVYERQVEKVDPLLDWVRSEFGFKPVVYSSFFGGKQE 240
           HL+KKFN DLVFCRAPE+NVLTSGVYERQVEK+DPLLDWV SEFGFKP+VYSSFFGG QE
Sbjct: 181 HLIKKFNSDLVFCRAPEENVLTSGVYERQVEKIDPLLDWVHSEFGFKPIVYSSFFGGNQE 240

Query: 241 DGLIKAVEDLLRKTDDCELASIDAIASAAHSLIIAIGIFRGKLQIEEAIELIRLEEDLQV 300
           DGLIKAVEDLL+KT+DCELASIDAIASAAHSLIIAIGIFRGKLQIEEAIELIRLEEDLQV
Sbjct: 241 DGLIKAVEDLLKKTNDCELASIDAIASAAHSLIIAIGIFRGKLQIEEAIELIRLEEDLQV 300

Query: 301 DKWGLVEGGHDVDIADLRVQISSATVFLALSRK 334
           DKWGLVEGGHDVDIADL+VQISSATVFLALSR+
Sbjct: 301 DKWGLVEGGHDVDIADLQVQISSATVFLALSRR 329

[Close]

BLAST of Lsi05G004730 vs. TrEMBL
Match: A0A067LGA4_JATCU (Uncharacterized protein OS=Jatropha curcas GN=JCGZ_10198 PE=4 SV=1)

HSP 1 Score: 490.7 bits (1262), Expect = 1.4e-135
Identity = 256/334 (76.65%), Postives = 291/334 (87.13%), Query Frame = 1


Query: 1   MASSILSKMAKSIRITNPTLIPFHSFRCRMLSTSVATAAEQAQSD-AASFTFSED-NARE 60
           MA+S++++  KSI+  NP L+   S       TS+ATA +    D ++SFTF+ +  A  
Sbjct: 1   MATSLINRTLKSIK--NPKLLSPLSSSLTRHFTSIATAQQSPSDDPSSSFTFTGNATATT 60

Query: 61  DPIFVKAPVSNSRADSSSPSSSSVTMPTSFMTGSVVGKRFYQQVTTREADDRNGWTVMLD 120
           D IF+KAP SN++ D++   SSSVTMP SFMTGS+VGKRFY+QVTTREADD NGWTVMLD
Sbjct: 61  DSIFLKAPNSNAKLDAAP--SSSVTMPMSFMTGSIVGKRFYKQVTTREADDGNGWTVMLD 120

Query: 121 YRTLKTPSKRPLKLPTLGLAKAVAAEWEYQETDGIRPFTMPLMKLACTALERVPLTRPKI 180
           YRTLKTPSKRPLKLPTL LAKA+AAEWEYQ+ DGIRPF MPLMKLACTALERVPLTRPKI
Sbjct: 121 YRTLKTPSKRPLKLPTLALAKAIAAEWEYQQLDGIRPFMMPLMKLACTALERVPLTRPKI 180

Query: 181 IEHLMKKFNQDLVFCRAPEDNVLTSGVYERQVEKVDPLLDWVRSEFGFKPVVYSSFFGGK 240
           IEHLMKKFNQDLVFCRAPEDN LT GVYERQVEK+DPLLDWV+SEFGFKPVV+SSFFGGK
Sbjct: 181 IEHLMKKFNQDLVFCRAPEDNDLTRGVYERQVEKIDPLLDWVKSEFGFKPVVHSSFFGGK 240

Query: 241 QEDGLIKAVEDLLRKTDDCELASIDAIASAAHSLIIAIGIFRGKLQIEEAIELIRLEEDL 300
           QE+GL+KA+EDLL+KTD+ ELASIDAIA+AAHSL+IA+GIFRGKL IEEAI+LIRLEEDL
Sbjct: 241 QEEGLVKAIEDLLQKTDNFELASIDAIAAAAHSLVIAVGIFRGKLDIEEAIQLIRLEEDL 300

Query: 301 QVDKWGLVEGGHDVDIADLRVQISSATVFLALSR 333
           QVDKWGLVEGGHDVDIADLRVQ+SSA VFL LSR
Sbjct: 301 QVDKWGLVEGGHDVDIADLRVQVSSAAVFLGLSR 330

[Close]

BLAST of Lsi05G004730 vs. TrEMBL
Match: A0A067ELJ3_CITSI (Uncharacterized protein OS=Citrus sinensis GN=CISIN_1g020035mg PE=4 SV=1)

HSP 1 Score: 482.6 bits (1241), Expect = 3.8e-133
Identity = 256/338 (75.74%), Postives = 290/338 (85.80%), Query Frame = 1


Query: 1   MASSILSKMAKSIRITNPTLIP-FHSFRCRMLSTSVATAAEQAQSD---AASFTFSEDNA 60
           MA+S++ K  +SIR  NP  I  F +FR R L  SVAT  +  Q D    +SFTFS  + 
Sbjct: 1   MAASLIPKTLQSIR--NPNAISSFTTFRTRHLC-SVATVHQSPQYDDDPTSSFTFSSPSD 60

Query: 61  R-EDPIFVKAPVSNSRADSSSPSSSSVTMPTSFMTGSVVGKRFYQQVTTREADDRNGWTV 120
           +  + I++K      + +++S SSSSVTMP SFMTGS+VGKRFY++V+TREADD NGWTV
Sbjct: 61  KINESIYIKG----RKQETASSSSSSVTMPMSFMTGSIVGKRFYEKVSTREADDGNGWTV 120

Query: 121 MLDYRTLKTPSKRPLKLPTLGLAKAVAAEWEYQETDGIRPFTMPLMKLACTALERVPLTR 180
           MLDYRTLKTPSKRPLKLPTLGLAKA+ AEW+YQ+TDGIRPF MPLMKLACTALERVPLTR
Sbjct: 121 MLDYRTLKTPSKRPLKLPTLGLAKAIGAEWDYQQTDGIRPFMMPLMKLACTALERVPLTR 180

Query: 181 PKIIEHLMKKFNQDLVFCRAPEDNVLTSGVYERQVEKVDPLLDWVRSEFGFKPVVYSSFF 240
           PKIIEHLMKKFNQDLVFCRAP DN LTSGV+ERQV+K+DPLL WV SEFGFKPVVYSSFF
Sbjct: 181 PKIIEHLMKKFNQDLVFCRAPADNDLTSGVHERQVQKIDPLLKWVESEFGFKPVVYSSFF 240

Query: 241 GGKQEDGLIKAVEDLLRKTDDCELASIDAIASAAHSLIIAIGIFRGKLQIEEAIELIRLE 300
           GGKQEDGLIK VE+L++KTDD ELA+IDAIA+AAHSL+IAIGIFRGKLQIEEAIELIRLE
Sbjct: 241 GGKQEDGLIKTVENLMKKTDDYELAAIDAIAAAAHSLVIAIGIFRGKLQIEEAIELIRLE 300

Query: 301 EDLQVDKWGLVEGGHDVDIADLRVQISSATVFLALSRK 334
           EDLQVDKWGLVEGGHD+DIADLRVQISSATVFL LSR+
Sbjct: 301 EDLQVDKWGLVEGGHDIDIADLRVQISSATVFLGLSRR 331

[Close]

BLAST of Lsi05G004730 vs. TrEMBL
Match: V4U8I3_9ROSI (Uncharacterized protein OS=Citrus clementina GN=CICLE_v10015882mg PE=4 SV=1)

HSP 1 Score: 481.9 bits (1239), Expect = 6.4e-133
Identity = 256/337 (75.96%), Postives = 286/337 (84.87%), Query Frame = 1


Query: 1   MASSILSKMAKSIRITNPTLIP-FHSFRCRMLSTSVATAAEQAQSD---AASFTFSEDNA 60
           MA+S++SK  +SIR  NP  I  F +FR R L  SVAT  +  Q D     SFTFS  + 
Sbjct: 1   MAASLISKTLQSIR--NPNAISSFTTFRTRHLC-SVATVHQSPQYDDDPTTSFTFSSPS- 60

Query: 61  REDPIFVKAPVSNSRADSSSPSSSSVTMPTSFMTGSVVGKRFYQQVTTREADDRNGWTVM 120
             D I     +   + +++S SSSSVTMP SFMTGS+VGKRFY++V+TREADD NGWTVM
Sbjct: 61  --DKINENIYIKGRKQETASSSSSSVTMPMSFMTGSIVGKRFYEKVSTREADDGNGWTVM 120

Query: 121 LDYRTLKTPSKRPLKLPTLGLAKAVAAEWEYQETDGIRPFTMPLMKLACTALERVPLTRP 180
           LDYRTLKTPSKRPLKLPTLGLAKA+ AEW+YQ+TDGIRPF MPLMKLACTALERVPLTRP
Sbjct: 121 LDYRTLKTPSKRPLKLPTLGLAKAIGAEWDYQQTDGIRPFMMPLMKLACTALERVPLTRP 180

Query: 181 KIIEHLMKKFNQDLVFCRAPEDNVLTSGVYERQVEKVDPLLDWVRSEFGFKPVVYSSFFG 240
           KIIEHLMKKFNQDLVFCRAP DN LTSGV+ERQV+K+DPLL WV SEF FKPVVYSSFFG
Sbjct: 181 KIIEHLMKKFNQDLVFCRAPADNDLTSGVHERQVQKIDPLLKWVESEFSFKPVVYSSFFG 240

Query: 241 GKQEDGLIKAVEDLLRKTDDCELASIDAIASAAHSLIIAIGIFRGKLQIEEAIELIRLEE 300
           GKQEDGLIK VE+L++KTDD ELA+IDAIA+AAHSL+IAIGIFRGKLQIEEAIELIRLEE
Sbjct: 241 GKQEDGLIKTVENLMKKTDDYELAAIDAIAAAAHSLVIAIGIFRGKLQIEEAIELIRLEE 300

Query: 301 DLQVDKWGLVEGGHDVDIADLRVQISSATVFLALSRK 334
           DLQVDKWGLVEGGHD+DIADLRVQISSATVFL LSR+
Sbjct: 301 DLQVDKWGLVEGGHDIDIADLRVQISSATVFLGLSRR 331

[Close]

BLAST of Lsi05G004730 vs. TAIR10
Match: AT5G40660.1 (AT5G40660.1 ATP12 protein-related)

HSP 1 Score: 429.5 bits (1103), Expect = 1.9e-120
Identity = 231/339 (68.14%), Postives = 276/339 (81.42%), Query Frame = 1


Query: 1   MASSILSKMAKSIRITNPTLIPFHSFRCRMLSTSVATAAEQAQSD------AASFTFSED 60
           MA+ ++ +  KS R +N  +      R R L T+  +AA Q  SD      ++SFTF ++
Sbjct: 1   MAAMLIGRAFKSARNSNLAI------RARSLCTT--SAARQPDSDTQPSESSSSFTFEKE 60

Query: 61  NAREDPIFVKAPVSNSRADSSSPSSSSVTMPTSFMTGSVVGKRFYQQVTTREADDRNGWT 120
           N  E PI VKAP  NSR  + S    SVTMPTSFMTGS+VGKRFY++VTTREADD NGWT
Sbjct: 61  N--EKPILVKAP--NSRRKNES---DSVTMPTSFMTGSIVGKRFYKKVTTREADDGNGWT 120

Query: 121 VMLDYRTLKTPSKRPLKLPTLGLAKAVAAEWEYQETDGIRPFTMPLMKLACTALERVPLT 180
           VMLDYRTLKTPSKRPLKL +L LAKA+AAEWEYQ T+GIRPFTMPLM+LACTALERVPLT
Sbjct: 121 VMLDYRTLKTPSKRPLKLRSLALAKAIAAEWEYQLTEGIRPFTMPLMRLACTALERVPLT 180

Query: 181 RPKIIEHLMKKFNQDLVFCRAPEDNVLTSGVYERQVEKVDPLLDWVRSEFGFKPVVYSSF 240
           R KIIEHL +K +QDLVF RAPEDN LTS V++ QVE +DPLL+W+ SEF  KP VYSS 
Sbjct: 181 RSKIIEHLSRKIHQDLVFFRAPEDNDLTSDVHDIQVESIDPLLEWIESEFRVKPKVYSSI 240

Query: 241 FGGKQEDGLIKAVEDLLRKTDDCELASIDAIASAAHSLIIAIGIFRGKLQIEEAIELIRL 300
           FGGKQ+D L+KAVE+LL+KT+D ELASIDA+ ++AHS++IA+GIF GKLQI++AI+LIRL
Sbjct: 241 FGGKQDDKLVKAVEELLKKTNDGELASIDALQASAHSIVIALGIFCGKLQIDDAIKLIRL 300

Query: 301 EEDLQVDKWGLVEGGHDVDIADLRVQISSATVFLALSRK 334
           EEDLQVDKWGLVEGGHD+D+ADL+VQISSATVFLALSR+
Sbjct: 301 EEDLQVDKWGLVEGGHDIDVADLKVQISSATVFLALSRE 324

[Close]

BLAST of Lsi05G004730 vs. NCBI nr
Match: gi|659098220|ref|XP_008450034.1| (PREDICTED: ATP synthase mitochondrial F1 complex assembly factor 2 isoform X1 [Cucumis melo])

HSP 1 Score: 575.9 bits (1483), Expect = 4.7e-161
Identity = 297/334 (88.92%), Postives = 314/334 (94.01%), Query Frame = 1


Query: 1   MASSILSKMAKSIRITNPTLIPFHSFRCRMLSTSVATAAEQAQSDAASFTFSEDNAREDP 60
           M++SILS MAKSIRITNPTL P HSFR RML TSVATA EQ+ SDAASFTFS DN RE+P
Sbjct: 1   MSTSILSIMAKSIRITNPTLTPSHSFRYRMLCTSVATAPEQSPSDAASFTFSNDNPREEP 60

Query: 61  IFVKAPVSNSRADSSSPSSSSVTMPTSFMTGSVVGKRFYQQVTTREADDRNGWTVMLDYR 120
           +F+KAP    R++SSSPSSSSVTMPTSFMTGS+VGKRFYQ+VTTREADD NGWTVMLDYR
Sbjct: 61  VFIKAP----RSNSSSPSSSSVTMPTSFMTGSIVGKRFYQKVTTREADDGNGWTVMLDYR 120

Query: 121 TLKTPSKRPLKLPTLGLAKAVAAEWEYQETDGIRPFTMPLMKLACTALERVPLTRPKIIE 180
           TLKTP+KRPLKLPTLGLAKAVAAEWEYQETDGIRPFTMPLMKLACTALERVPLTRPKIIE
Sbjct: 121 TLKTPTKRPLKLPTLGLAKAVAAEWEYQETDGIRPFTMPLMKLACTALERVPLTRPKIIE 180

Query: 181 HLMKKFNQDLVFCRAPEDNVLTSGVYERQVEKVDPLLDWVRSEFGFKPVVYSSFFGGKQE 240
           HLM KFN+DLVFCRAPED+ LTSGVYERQVEK+DPLLDWV SEFGFKP+VYSS FGG QE
Sbjct: 181 HLMGKFNRDLVFCRAPEDD-LTSGVYERQVEKIDPLLDWVHSEFGFKPIVYSSLFGGNQE 240

Query: 241 DGLIKAVEDLLRKTDDCELASIDAIASAAHSLIIAIGIFRGKLQIEEAIELIRLEEDLQV 300
           DGLIKAVEDLLRKTDDCELASIDAIASAAHSLIIAIGIFRGKLQIEEAIELIRLEED QV
Sbjct: 241 DGLIKAVEDLLRKTDDCELASIDAIASAAHSLIIAIGIFRGKLQIEEAIELIRLEEDFQV 300

Query: 301 DKWGLVEGGHDVDIADLRVQISSATVFLALSRKF 335
           DKWGLVEGGHDVDIADL+VQISSATVFLALSR+F
Sbjct: 301 DKWGLVEGGHDVDIADLQVQISSATVFLALSRRF 329

[Close]

BLAST of Lsi05G004730 vs. NCBI nr
Match: gi|449455898|ref|XP_004145687.1| (PREDICTED: ATP synthase mitochondrial F1 complex assembly factor 2 [Cucumis sativus])

HSP 1 Score: 572.0 bits (1473), Expect = 6.8e-160
Identity = 295/333 (88.59%), Postives = 311/333 (93.39%), Query Frame = 1


Query: 1   MASSILSKMAKSIRITNPTLIPFHSFRCRMLSTSVATAAEQAQSDAASFTFSEDNAREDP 60
           M+SSILSKMAKSIRITNPTL P HSFR RML TSVATAAEQ+  D  SFTFS DN R++P
Sbjct: 1   MSSSILSKMAKSIRITNPTLSPSHSFRYRMLCTSVATAAEQSPLDDTSFTFSNDNPRDEP 60

Query: 61  IFVKAPVSNSRADSSSPSSSSVTMPTSFMTGSVVGKRFYQQVTTREADDRNGWTVMLDYR 120
           +FVKAP SNS    SS SSSSVTMPTSFMTGS+VGKRFYQ+VTTREADDRNGW VMLDYR
Sbjct: 61  VFVKAPRSNS----SSRSSSSVTMPTSFMTGSIVGKRFYQKVTTREADDRNGWAVMLDYR 120

Query: 121 TLKTPSKRPLKLPTLGLAKAVAAEWEYQETDGIRPFTMPLMKLACTALERVPLTRPKIIE 180
           TLKTP+KRPLKLPTLGLAKAVAAEWEYQETDGIRPFTMPLMKLACTALERVPLTR  IIE
Sbjct: 121 TLKTPTKRPLKLPTLGLAKAVAAEWEYQETDGIRPFTMPLMKLACTALERVPLTRHTIIE 180

Query: 181 HLMKKFNQDLVFCRAPEDNVLTSGVYERQVEKVDPLLDWVRSEFGFKPVVYSSFFGGKQE 240
           HL+KKFN DLVFCRAPE+NVLTSGVYERQVEK+DPLLDWV SEFGFKP+VYSSFFGG QE
Sbjct: 181 HLIKKFNSDLVFCRAPEENVLTSGVYERQVEKIDPLLDWVHSEFGFKPIVYSSFFGGNQE 240

Query: 241 DGLIKAVEDLLRKTDDCELASIDAIASAAHSLIIAIGIFRGKLQIEEAIELIRLEEDLQV 300
           DGLIKAVEDLL+KT+DCELASIDAIASAAHSLIIAIGIFRGKLQIEEAIELIRLEEDLQV
Sbjct: 241 DGLIKAVEDLLKKTNDCELASIDAIASAAHSLIIAIGIFRGKLQIEEAIELIRLEEDLQV 300

Query: 301 DKWGLVEGGHDVDIADLRVQISSATVFLALSRK 334
           DKWGLVEGGHDVDIADL+VQISSATVFLALSR+
Sbjct: 301 DKWGLVEGGHDVDIADLQVQISSATVFLALSRR 329

[Close]

BLAST of Lsi05G004730 vs. NCBI nr
Match: gi|659098222|ref|XP_008450035.1| (PREDICTED: ATP synthase mitochondrial F1 complex assembly factor 2 isoform X2 [Cucumis melo])

HSP 1 Score: 510.0 bits (1312), Expect = 3.2e-141
Identity = 264/299 (88.29%), Postives = 279/299 (93.31%), Query Frame = 1


Query: 1   MASSILSKMAKSIRITNPTLIPFHSFRCRMLSTSVATAAEQAQSDAASFTFSEDNAREDP 60
           M++SILS MAKSIRITNPTL P HSFR RML TSVATA EQ+ SDAASFTFS DN RE+P
Sbjct: 1   MSTSILSIMAKSIRITNPTLTPSHSFRYRMLCTSVATAPEQSPSDAASFTFSNDNPREEP 60

Query: 61  IFVKAPVSNSRADSSSPSSSSVTMPTSFMTGSVVGKRFYQQVTTREADDRNGWTVMLDYR 120
           +F+KAP    R++SSSPSSSSVTMPTSFMTGS+VGKRFYQ+VTTREADD NGWTVMLDYR
Sbjct: 61  VFIKAP----RSNSSSPSSSSVTMPTSFMTGSIVGKRFYQKVTTREADDGNGWTVMLDYR 120

Query: 121 TLKTPSKRPLKLPTLGLAKAVAAEWEYQETDGIRPFTMPLMKLACTALERVPLTRPKIIE 180
           TLKTP+KRPLKLPTLGLAKAVAAEWEYQETDGIRPFTMPLMKLACTALERVPLTRPKIIE
Sbjct: 121 TLKTPTKRPLKLPTLGLAKAVAAEWEYQETDGIRPFTMPLMKLACTALERVPLTRPKIIE 180

Query: 181 HLMKKFNQDLVFCRAPEDNVLTSGVYERQVEKVDPLLDWVRSEFGFKPVVYSSFFGGKQE 240
           HLM KFN+DLVFCRAPED+ LTSGVYERQVEK+DPLLDWV SEFGFKP+VYSS FGG QE
Sbjct: 181 HLMGKFNRDLVFCRAPEDD-LTSGVYERQVEKIDPLLDWVHSEFGFKPIVYSSLFGGNQE 240

Query: 241 DGLIKAVEDLLRKTDDCELASIDAIASAAHSLIIAIGIFRGKLQIEEAIELIRLEEDLQ 300
           DGLIKAVEDLLRKTDDCELASIDAIASAAHSLIIAIGIFRGKLQIEEAIELIRLEED Q
Sbjct: 241 DGLIKAVEDLLRKTDDCELASIDAIASAAHSLIIAIGIFRGKLQIEEAIELIRLEEDFQ 294

[Close]

BLAST of Lsi05G004730 vs. NCBI nr
Match: gi|1009124344|ref|XP_015879016.1| (PREDICTED: ATP synthase mitochondrial F1 complex assembly factor 2 [Ziziphus jujuba])

HSP 1 Score: 493.8 bits (1270), Expect = 2.3e-136
Identity = 263/339 (77.58%), Postives = 293/339 (86.43%), Query Frame = 1


Query: 1   MASSILSKMAKSIRITNPTLIPFHSFRCRMLSTSVATAAEQAQSD--AASFTFSEDNARE 60
           MA+S++ K   S+R  NP L    S R   +S S+ATA EQ QSD  + SFTFS D  +E
Sbjct: 1   MANSLIRKTLISVR--NPLLFKSASSRNCYIS-SIATA-EQPQSDGPSLSFTFSSDGQQE 60

Query: 61  ----DPIFVKAPVSNSRADSSSPSSSSVTMPTSFMTGSVVGKRFYQQVTTREADDRNGWT 120
               + I+VKAP SN R +++S SSSSVTMP SFMTGS+VGKRFY+QVT READD NGWT
Sbjct: 61  HKADNNIYVKAPSSNPRRETAS-SSSSVTMPMSFMTGSIVGKRFYKQVTIREADDGNGWT 120

Query: 121 VMLDYRTLKTPSKRPLKLPTLGLAKAVAAEWEYQETDGIRPFTMPLMKLACTALERVPLT 180
           VMLDYRTLKTPSKRPLKLPT  LAKA+AAEWEYQ+TDGIRPFTMPLMKLACTA ERVPLT
Sbjct: 121 VMLDYRTLKTPSKRPLKLPTSSLAKAIAAEWEYQQTDGIRPFTMPLMKLACTASERVPLT 180

Query: 181 RPKIIEHLMKKFNQDLVFCRAPEDNVLTSGVYERQVEKVDPLLDWVRSEFGFKPVVYSSF 240
           RPKIIEHL+KKFNQDLVFCRAPEDN LTS VYE+QVEK+DPLLDWV SEFGFKPVVYSSF
Sbjct: 181 RPKIIEHLIKKFNQDLVFCRAPEDNDLTSNVYEKQVEKIDPLLDWVASEFGFKPVVYSSF 240

Query: 241 FGGKQEDGLIKAVEDLLRKTDDCELASIDAIASAAHSLIIAIGIFRGKLQIEEAIELIRL 300
           FGGKQEDGL+KA+E+ L++TDDCELA +DAIASAAHSL+IA+G+FRGKLQIEEAIELIRL
Sbjct: 241 FGGKQEDGLVKAMENFLKRTDDCELAVVDAIASAAHSLVIAVGLFRGKLQIEEAIELIRL 300

Query: 301 EEDLQVDKWGLVEGGHDVDIADLRVQISSATVFLALSRK 334
           EEDLQVD+WGLVEGGHDVDIADLRVQISSA VFL LSRK
Sbjct: 301 EEDLQVDRWGLVEGGHDVDIADLRVQISSAVVFLGLSRK 334

[Close]

BLAST of Lsi05G004730 vs. NCBI nr
Match: gi|802539509|ref|XP_012071373.1| (PREDICTED: ATP synthase mitochondrial F1 complex assembly factor 2 [Jatropha curcas])

HSP 1 Score: 490.7 bits (1262), Expect = 2.0e-135
Identity = 256/334 (76.65%), Postives = 291/334 (87.13%), Query Frame = 1


Query: 1   MASSILSKMAKSIRITNPTLIPFHSFRCRMLSTSVATAAEQAQSD-AASFTFSED-NARE 60
           MA+S++++  KSI+  NP L+   S       TS+ATA +    D ++SFTF+ +  A  
Sbjct: 1   MATSLINRTLKSIK--NPKLLSPLSSSLTRHFTSIATAQQSPSDDPSSSFTFTGNATATT 60

Query: 61  DPIFVKAPVSNSRADSSSPSSSSVTMPTSFMTGSVVGKRFYQQVTTREADDRNGWTVMLD 120
           D IF+KAP SN++ D++   SSSVTMP SFMTGS+VGKRFY+QVTTREADD NGWTVMLD
Sbjct: 61  DSIFLKAPNSNAKLDAAP--SSSVTMPMSFMTGSIVGKRFYKQVTTREADDGNGWTVMLD 120

Query: 121 YRTLKTPSKRPLKLPTLGLAKAVAAEWEYQETDGIRPFTMPLMKLACTALERVPLTRPKI 180
           YRTLKTPSKRPLKLPTL LAKA+AAEWEYQ+ DGIRPF MPLMKLACTALERVPLTRPKI
Sbjct: 121 YRTLKTPSKRPLKLPTLALAKAIAAEWEYQQLDGIRPFMMPLMKLACTALERVPLTRPKI 180

Query: 181 IEHLMKKFNQDLVFCRAPEDNVLTSGVYERQVEKVDPLLDWVRSEFGFKPVVYSSFFGGK 240
           IEHLMKKFNQDLVFCRAPEDN LT GVYERQVEK+DPLLDWV+SEFGFKPVV+SSFFGGK
Sbjct: 181 IEHLMKKFNQDLVFCRAPEDNDLTRGVYERQVEKIDPLLDWVKSEFGFKPVVHSSFFGGK 240

Query: 241 QEDGLIKAVEDLLRKTDDCELASIDAIASAAHSLIIAIGIFRGKLQIEEAIELIRLEEDL 300
           QE+GL+KA+EDLL+KTD+ ELASIDAIA+AAHSL+IA+GIFRGKL IEEAI+LIRLEEDL
Sbjct: 241 QEEGLVKAIEDLLQKTDNFELASIDAIAAAAHSLVIAVGIFRGKLDIEEAIQLIRLEEDL 300

Query: 301 QVDKWGLVEGGHDVDIADLRVQISSATVFLALSR 333
           QVDKWGLVEGGHDVDIADLRVQ+SSA VFL LSR
Sbjct: 301 QVDKWGLVEGGHDVDIADLRVQVSSAAVFLGLSR 330

The following BLAST results are available for this feature:

BLAST of Lsi05G004730 vs. Swiss-Prot


Match Name	E-value	Identity	Description
ATPF2_HUMAN	2.6e-26	31.22	ATP synthase mitochondrial F1 complex assembly factor 2 OS=Homo sapiens GN=ATPAF...	[more]
ATPF2_MOUSE	1.3e-25	30.80	ATP synthase mitochondrial F1 complex assembly factor 2 OS=Mus musculus GN=Atpaf...	[more]
ATPF2_BOVIN	1.1e-24	31.22	ATP synthase mitochondrial F1 complex assembly factor 2 OS=Bos taurus GN=ATPAF2 ...	[more]
ATP12_SCHPO	6.6e-14	25.00	Protein atp12, mitochondrial OS=Schizosaccharomyces pombe (strain 972 / ATCC 248...	[more]
ATP12_YEAST	6.6e-06	21.43	Protein ATP12, mitochondrial OS=Saccharomyces cerevisiae (strain ATCC 204508 / S...	[more]

BLAST of Lsi05G004730 vs. TrEMBL


Match Name	E-value	Identity	Description
E5GCD8_CUCME	3.3e-161	88.92	ATP12-like protein OS=Cucumis melo subsp. melo PE=4 SV=1	[more]
A0A0A0L8R2_CUCSA	4.7e-160	88.59	Uncharacterized protein OS=Cucumis sativus GN=Csa_3G595240 PE=4 SV=1	[more]
A0A067LGA4_JATCU	1.4e-135	76.65	Uncharacterized protein OS=Jatropha curcas GN=JCGZ_10198 PE=4 SV=1	[more]
A0A067ELJ3_CITSI	3.8e-133	75.74	Uncharacterized protein OS=Citrus sinensis GN=CISIN_1g020035mg PE=4 SV=1	[more]
V4U8I3_9ROSI	6.4e-133	75.96	Uncharacterized protein OS=Citrus clementina GN=CICLE_v10015882mg PE=4 SV=1	[more]

BLAST of Lsi05G004730 vs. TAIR10


Match Name	E-value	Identity	Description
AT5G40660.1	1.9e-120	68.14	ATP12 protein-related	[more]

BLAST of Lsi05G004730 vs. NCBI nr


Match Name	E-value	Identity	Description
gi\|659098220\|ref\|XP_008450034.1\|	4.7e-161	88.92	PREDICTED: ATP synthase mitochondrial F1 complex assembly factor 2 isoform X1 [C...	[more]
gi\|449455898\|ref\|XP_004145687.1\|	6.8e-160	88.59	PREDICTED: ATP synthase mitochondrial F1 complex assembly factor 2 [Cucumis sati...	[more]
gi\|659098222\|ref\|XP_008450035.1\|	3.2e-141	88.29	PREDICTED: ATP synthase mitochondrial F1 complex assembly factor 2 isoform X2 [C...	[more]
gi\|1009124344\|ref\|XP_015879016.1\|	2.3e-136	77.58	PREDICTED: ATP synthase mitochondrial F1 complex assembly factor 2 [Ziziphus juj...	[more]
gi\|802539509\|ref\|XP_012071373.1\|	2.0e-135	76.65	PREDICTED: ATP synthase mitochondrial F1 complex assembly factor 2 [Jatropha cur...	[more]

Annotated Terms

The following terms have been associated with this gene:

Vocabulary: **Biological Process**
Term	Definition
GO:0043461	proton-transporting ATP synthase complex assembly

Vocabulary: **INTERPRO**
Term	Definition
IPR023335	ATP12_ortho_dom_sf
IPR011419	ATP12_ATP_synth-F1-assembly

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0043461	proton-transporting ATP synthase complex assembly
cellular_component	GO:0005739	mitochondrion
cellular_component	GO:0005575	cellular_component
molecular_function	GO:0003674	molecular_function

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Lsi05G004730.1	Lsi05G004730.1	mRNA

InterPro

Analysis Name: InterPro Annotations of Lagenaria siceraria
Date Performed: 2017-09-18


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR011419	ATP12, ATPase F1F0-assembly protein	PANTHER	PTHR21013	ATP SYNTHASE MITOCHONDRIAL F1 COMPLEX ASSEMBLY FACTOR 2/ATP12 PROTEIN, MITOCHONDRIAL PRECURSOR	coord: 78..331 score: 6.2E
IPR011419	ATP12, ATPase F1F0-assembly protein	PFAM	PF07542	ATP12	coord: 96..220 score: 5.8
IPR023335	Orthogonal Bundle domain in ATP12	GENE3D	G3DSA:1.10.3580.10		coord: 158..330 score: 2.2
None	No IPR available	GENE3D	G3DSA:3.30.2180.10		coord: 73..155 score: 4.3
None	No IPR available	unknown	SSF160909	ATP12-like	coord: 83..329 score: 3.53

Synteny

The following gene(s) are orthologous to this gene:


Gene	Orthologue	Organism	Block
Lsi05G004730	Csa3G595240	Cucumber (Chinese Long) v2	culsiB214
Lsi05G004730	CSPI03G26020	Wild cucumber (PI 183967)	cpilsiB212
Lsi05G004730	Cucsa.156210	Cucumber (Gy14) v1	cgylsiB248
Lsi05G004730	CmaCh14G006850	Cucurbita maxima (Rimu)	cmalsiB248
Lsi05G004730	CmoCh14G007020	Cucurbita moschata (Rifu)	cmolsiB236
Lsi05G004730	MELO3C014823	Melon (DHL92) v3.5.1	lsimeB368
Lsi05G004730	ClCG05G013000	Watermelon (Charleston Gray)	lsiwcgB332
Lsi05G004730	MELO3C014823.2	Melon (DHL92) v3.6.1	lsimedB402
Lsi05G004730	Cla97C05G094570	Watermelon (97103) v2	lsiwmbB329
Lsi05G004730	Bhi01G001556	Wax gourd	lsiwgoB436
Lsi05G004730	Cp4.1LG03g07180	Cucurbita pepo (Zucchini)	cpelsiB509
Lsi05G004730	CsGy3G024370	Cucumber (Gy14) v2	cgyblsiB201
Lsi05G004730	Carg09693	Silver-seed gourd	carlsiB396
Lsi05G004730	CsaV3_3G027540	Cucumber (Chinese Long) v3	cuclsiB233

The following gene(s) are paralogous to this gene:

None

The following block(s) are covering this gene:


Gene	Organism	Block
Lsi05G004730	Cucurbita maxima (Rimu)	cmalsiB125
Lsi05G004730	Cucurbita moschata (Rifu)	cmolsiB117
Lsi05G004730	Cucurbita pepo (Zucchini)	cpelsiB511
Lsi05G004730	Cucurbita pepo (Zucchini)	cpelsiB555
Lsi05G004730	Silver-seed gourd	carlsiB334