Lsi05G003740 (gene) Bottle gourd (USVL1VR-Ls) | Cucurbit Genomics Database (CuGenDB)

Overview


Name	Lsi05G003740
Type	gene
Organism	Lagenaria siceraria (Bottle gourd (USVL1VR-Ls))
Description	AGAMOUS MADS box factor transcription factor
Location	chr05 : 4734847 .. 4735809 (+)

Genome Viewer

Gene Expression

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: CDS

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGGGCAGAGGAATATTGAGCCTTAAACTGATTCCCAATCTCAAATCTCGCAGAACAACCTTTCTTAAGAGGAAGAAAAGCCTGATGAAGAAGGCCTACGAGCTTTCCACTCTTTGCGATGTTCAAACCTGCGTTTTCATCGCTTCTGATTGTGACCCATCAACCCAATTTGAAACTTGGCCTCAAAATCACGATCAAATCCAGGCTATGATCCGTTCCTATAAATCTCACTCTTTCCCCAAACAATCCCGCAACTCCTCTTACGATCTCCAACAATTCTTCTCTGATCGCAAGAACAAAATCGTAACAGAGACATCTAAACTGCGTCAGAATGTTGACAAATTCAAGGATCCCCACCTTGATTCTCTATCGGAACAGCAATTGAAGGAGTTGTTGGCCGCTTTGGATTCCAAAATTGGAGATGCAGACGGCATGATTCAGTTCATGGAGGCCGATTACGATCATTTGATTGAGGAAGCATCTGGAATTTCAAGCATTAACATACCACCATTCAGTCCTTTCGAAACAGAAGAACCCCCTAAGTTAGACATACCGGAGTCGTTGATTGAGCCATATGGGTTTGAATACAATGTCGAGAAGTTCATGGAGGCCGATTACGATCACTTGATTGTGGAAGCATCTGAAATTTCAAGCATTGACCCACCATCATCCAGTTCTTTTGAAACAAAGGAACCCCCAGAATTAGACATATCGGAGTTGTTGATTTGGCCAGATGGGTTTGAATACAATGTCGAGGAGTTTACGGAGGCCGATTGCGATCACTTGTTTGAGGAAGCATCTAGACTTTCAAGCATTGACACACCATCATTCAGTCCTTTCGAAATAGAGGAACCCCCTGGATTAGACATATCGGAGTTGTTGATTTTGGCAGATGAGTTTGAATACAATGTCCAGGATTTTCAATCTCTATTGGAGGACGAGCTTCTCGAAACCCTGCTTTAG

mRNA sequence

ATGGGCAGAGGAATATTGAGCCTTAAACTGATTCCCAATCTCAAATCTCGCAGAACAACCTTTCTTAAGAGGAAGAAAAGCCTGATGAAGAAGGCCTACGAGCTTTCCACTCTTTGCGATGTTCAAACCTGCGTTTTCATCGCTTCTGATTGTGACCCATCAACCCAATTTGAAACTTGGCCTCAAAATCACGATCAAATCCAGGCTATGATCCGTTCCTATAAATCTCACTCTTTCCCCAAACAATCCCGCAACTCCTCTTACGATCTCCAACAATTCTTCTCTGATCGCAAGAACAAAATCGTAACAGAGACATCTAAACTGCGTCAGAATGTTGACAAATTCAAGGATCCCCACCTTGATTCTCTATCGGAACAGCAATTGAAGGAGTTGTTGGCCGCTTTGGATTCCAAAATTGGAGATGCAGACGGCATGATTCAGTTCATGGAGGCCGATTACGATCATTTGATTGAGGAAGCATCTGGAATTTCAAGCATTAACATACCACCATTCAGTCCTTTCGAAACAGAAGAACCCCCTAAGTTAGACATACCGGAGTCGTTGATTGAGCCATATGGGTTTGAATACAATGTCGAGAAGTTCATGGAGGCCGATTACGATCACTTGATTGTGGAAGCATCTGAAATTTCAAGCATTGACCCACCATCATCCAGTTCTTTTGAAACAAAGGAACCCCCAGAATTAGACATATCGGAGTTGTTGATTTGGCCAGATGGGTTTGAATACAATGTCGAGGAGTTTACGGAGGCCGATTGCGATCACTTGTTTGAGGAAGCATCTAGACTTTCAAGCATTGACACACCATCATTCAGTCCTTTCGAAATAGAGGAACCCCCTGGATTAGACATATCGGAGTTGTTGATTTTGGCAGATGAGTTTGAATACAATGTCCAGGATTTTCAATCTCTATTGGAGGACGAGCTTCTCGAAACCCTGCTTTAG

Coding sequence (CDS)

ATGGGCAGAGGAATATTGAGCCTTAAACTGATTCCCAATCTCAAATCTCGCAGAACAACCTTTCTTAAGAGGAAGAAAAGCCTGATGAAGAAGGCCTACGAGCTTTCCACTCTTTGCGATGTTCAAACCTGCGTTTTCATCGCTTCTGATTGTGACCCATCAACCCAATTTGAAACTTGGCCTCAAAATCACGATCAAATCCAGGCTATGATCCGTTCCTATAAATCTCACTCTTTCCCCAAACAATCCCGCAACTCCTCTTACGATCTCCAACAATTCTTCTCTGATCGCAAGAACAAAATCGTAACAGAGACATCTAAACTGCGTCAGAATGTTGACAAATTCAAGGATCCCCACCTTGATTCTCTATCGGAACAGCAATTGAAGGAGTTGTTGGCCGCTTTGGATTCCAAAATTGGAGATGCAGACGGCATGATTCAGTTCATGGAGGCCGATTACGATCATTTGATTGAGGAAGCATCTGGAATTTCAAGCATTAACATACCACCATTCAGTCCTTTCGAAACAGAAGAACCCCCTAAGTTAGACATACCGGAGTCGTTGATTGAGCCATATGGGTTTGAATACAATGTCGAGAAGTTCATGGAGGCCGATTACGATCACTTGATTGTGGAAGCATCTGAAATTTCAAGCATTGACCCACCATCATCCAGTTCTTTTGAAACAAAGGAACCCCCAGAATTAGACATATCGGAGTTGTTGATTTGGCCAGATGGGTTTGAATACAATGTCGAGGAGTTTACGGAGGCCGATTGCGATCACTTGTTTGAGGAAGCATCTAGACTTTCAAGCATTGACACACCATCATTCAGTCCTTTCGAAATAGAGGAACCCCCTGGATTAGACATATCGGAGTTGTTGATTTTGGCAGATGAGTTTGAATACAATGTCCAGGATTTTCAATCTCTATTGGAGGACGAGCTTCTCGAAACCCTGCTTTAG

Protein sequence

MGRGILSLKLIPNLKSRRTTFLKRKKSLMKKAYELSTLCDVQTCVFIASDCDPSTQFETWPQNHDQIQAMIRSYKSHSFPKQSRNSSYDLQQFFSDRKNKIVTETSKLRQNVDKFKDPHLDSLSEQQLKELLAALDSKIGDADGMIQFMEADYDHLIEEASGISSINIPPFSPFETEEPPKLDIPESLIEPYGFEYNVEKFMEADYDHLIVEASEISSIDPPSSSSFETKEPPELDISELLIWPDGFEYNVEEFTEADCDHLFEEASRLSSIDTPSFSPFEIEEPPGLDISELLILADEFEYNVQDFQSLLEDELLETLL

Homology

[Close]

BLAST of Lsi05G003740 vs. Swiss-Prot
Match: AGL82_ARATH (Agamous-like MADS-box protein AGL82 OS=Arabidopsis thaliana GN=AGL82 PE=1 SV=1)

HSP 1 Score: 73.6 bits (179), Expect = 4.5e-12
Identity = 66/260 (25.38%), Postives = 121/260 (46.54%), Query Frame = 1


Query: 5   ILSLKLIPNLKSRRTTFLKRKKSLMKKAYELSTLCDVQTCVFIASDCDPS----TQFETW 64
           ++ L+ I N K+R TT+ KRK SL KKA E STLC V+TC+ +      +    ++ E W
Sbjct: 5   VVDLQRIANDKTRITTYKKRKASLYKKAQEFSTLCGVETCLIVYGPTKATDVVISEPEIW 64

Query: 65  PQNHDQIQAMIRSYKSHSFPKQSRNSSYDLQQFFSD--RKNKIVTETSKLRQNVDKFKDP 124
           P++  +++A+IR YK       S     +++ F +D  + N++VT+    R+N     + 
Sbjct: 65  PKDETKVRAIIRKYK--DTVSTSCRKETNVETFVNDVGKGNEVVTKKRVKRENKYSSWEE 124

Query: 125 HLDSLSEQQLKELLAALDSKIGDADGMIQFMEADYDHLIEEASGISSINIPPFSPFETEE 184
            LD  S +QL  +  A+DSK+ +A    +      +H   +     ++    F P    E
Sbjct: 125 KLDKCSREQLHGIFCAVDSKLNEAVTRQERSMFRVNHQAMDTPFPQNLMDQQFMPQYFHE 184

Query: 185 PPKL--------DIPESLIEPYGFEYNVEKFMEADYDHLIVEASEISSIDPPSSSSFETK 244
            P+         ++  SLI P+  +  ++  +   +  L +  S + S     +   + +
Sbjct: 185 QPQFQGFPNNFNNMGFSLISPHDGQIQMDPNLMEKWTDLALTQSLMMSKGNDGTQFMQRQ 244

Query: 245 EPPELDISELLIWPDGFEYN 251
           E P  +  +++    GF  N
Sbjct: 245 EQPYYNREQVVSRSAGFNVN 262

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BLAST of Lsi05G003740 vs. Swiss-Prot
Match: AG_PANGI (Floral homeotic protein AGAMOUS OS=Panax ginseng GN=AG2 PE=2 SV=1)

HSP 1 Score: 63.2 bits (152), Expect = 6.1e-09
Identity = 46/157 (29.30%), Postives = 77/157 (49.04%), Query Frame = 1


Query: 1   MGRGILSLKLIPNLKSRRTTFLKRKKSLMKKAYELSTLCDVQTCVFIASDCDPSTQFETW 60
           +GRG + +K I N  +R+ TF KR+  L+KKAYELS LCD +  + +      ST+   +
Sbjct: 17  LGRGKIEIKRIENTTNRQVTFCKRRNGLLKKAYELSVLCDAEVALIVF-----STRGRLY 76

Query: 61  PQNHDQIQAMIRSYKSHSFPKQSRNSSYDLQ-QFFSDRKNKIVTETSKLRQNVDKFKDPH 120
              ++ ++  I  YK       + +S  +   QF+    +K+  E S +++N        
Sbjct: 77  EYANNSVKGTIERYKKACTDSPNTSSVSEANAQFYQQEASKLRQEISSIQKNNRNMMGES 136

Query: 121 LDSLSEQQLKELLAALD---SKIGDADGMIQFMEADY 154
           L SL+ + LK L   L+   S+I      + F E +Y
Sbjct: 137 LGSLTVRDLKGLETKLEKGISRIRSKKNELLFAEIEY 168

[Close]

BLAST of Lsi05G003740 vs. Swiss-Prot
Match: AG103_ARATH (Agamous-like MADS-box protein AGL103 OS=Arabidopsis thaliana GN=AGL103 PE=1 SV=1)

HSP 1 Score: 60.1 bits (144), Expect = 5.2e-08
Identity = 43/132 (32.58%), Postives = 72/132 (54.55%), Query Frame = 1


Query: 16  SRRTTFLKRKKSLMKKAYELSTLCDVQTCVFIASDCDPSTQFETWPQNHDQIQAMIRSYK 75
           SR T+ +KR++++ KKA ELS LCD+  CV        + + +TWP+  ++++A+ R Y 
Sbjct: 31  SRATSLIKRQQTVFKKAKELSILCDIDVCVICYGS---NGELKTWPEEREKVKAIARRYG 90

Query: 76  SHSFPKQSRNSSYDLQQFFSDRKNKIVTETSKLR----QNVDKFK----DPHLDSLSEQQ 135
             S  K+ R  S DL +F  ++ NK   E  + +    + V K K    DP  D+ S +Q
Sbjct: 91  ELSETKR-RKGSVDLHEFL-EKMNKDDPEKEEKKKIKVRRVPKVKYPVWDPRFDNYSVEQ 150

Query: 136 LKELLAALDSKI 140
           L  L+ +L+  +
Sbjct: 151 LMGLVQSLERNL 157

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BLAST of Lsi05G003740 vs. Swiss-Prot
Match: MAD13_ORYSJ (MADS-box transcription factor 13 OS=Oryza sativa subsp. japonica GN=MADS13 PE=1 SV=2)

HSP 1 Score: 59.7 bits (143), Expect = 6.8e-08
Identity = 43/142 (30.28%), Postives = 74/142 (52.11%), Query Frame = 1


Query: 1   MGRGILSLKLIPNLKSRRTTFLKRKKSLMKKAYELSTLCDVQTCVFIASDCDPSTQFETW 60
           MGRG + +K I N  SR+ TF KR+  L+KKAYELS LCD +  + + S      ++   
Sbjct: 1   MGRGRIEIKRIENTTSRQVTFCKRRNGLLKKAYELSVLCDAEVALIVFSSRGRLYEY--- 60

Query: 61  PQNHDQIQAMIRSY-KSHSFPKQSRNSSYDL--QQFFSDRKNKIVTETSKLRQNVDKFKD 120
             N++ ++A I  Y K+H+    S     ++  QQ++     K+  +   L+        
Sbjct: 61  -SNNNNVKATIDRYKKAHACGSTSGAPLIEVNAQQYYQQESAKLRHQIQMLQNTNKHLVG 120

Query: 121 PHLDSLSEQQLKELLAALDSKI 140
            ++ +LS ++LK+L + L+  I
Sbjct: 121 DNVSNLSLKELKQLESRLEKGI 138

[Close]

BLAST of Lsi05G003740 vs. Swiss-Prot
Match: AGL11_ARATH (Agamous-like MADS-box protein AGL11 OS=Arabidopsis thaliana GN=AGL11 PE=1 SV=1)

HSP 1 Score: 57.8 bits (138), Expect = 2.6e-07
Identity = 41/135 (30.37%), Postives = 70/135 (51.85%), Query Frame = 1


Query: 1   MGRGILSLKLIPNLKSRRTTFLKRKKSLMKKAYELSTLCDVQTCVFIASDCDPSTQFETW 60
           MGRG + +K I N  +R+ TF KR+  L+KKAYELS LCD +  + +      ST+   +
Sbjct: 1   MGRGKIEIKRIENSTNRQVTFCKRRNGLLKKAYELSVLCDAEVALIVF-----STRGRLY 60

Query: 61  PQNHDQIQAMIRSYKSHSFPKQSRNSSYDLQQFFSDRKNKIVTETSKLRQNVDKFKDPHL 120
              ++ I++ I  YK       + ++  ++   +  +      E++KLRQ +   ++ + 
Sbjct: 61  EYANNNIRSTIERYKKACSDSTNTSTVQEINAAYYQQ------ESAKLRQQIQTIQNSNR 120

Query: 121 ----DSLSEQQLKEL 132
               DSLS   +KEL
Sbjct: 121 NLMGDSLSSLSVKEL 124

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BLAST of Lsi05G003740 vs. TrEMBL
Match: A0A0A0LAL9_CUCSA (Uncharacterized protein OS=Cucumis sativus GN=Csa_3G258140 PE=4 SV=1)

HSP 1 Score: 238.4 bits (607), Expect = 1.2e-59
Identity = 141/224 (62.95%), Postives = 161/224 (71.88%), Query Frame = 1


Query: 1   MGRGILSLKLIPNLKSRRTTFLKRKKSLMKKAYELSTLCDVQTCVFIASDCDPSTQFETW 60
           MGRGILSLKLIPN KSRRTTFLKRKKSL+KKAYELSTLCDVQTC+FIASDCDPST FETW
Sbjct: 1   MGRGILSLKLIPNPKSRRTTFLKRKKSLIKKAYELSTLCDVQTCLFIASDCDPSTHFETW 60

Query: 61  PQNHDQIQAMIRSYKSHSFPKQSRNSSYDLQQFFSDRKNKIVTETSKLRQNVDKFKDPHL 120
           P NH QI  MIRSYKSHSF K   NSSYDL +FFSDRKNKI+T TSKL  NV       +
Sbjct: 61  PPNHHQIHQMIRSYKSHSFTKP--NSSYDLNRFFSDRKNKILTNTSKLLHNV-------V 120

Query: 121 DSLSEQQLKELLAALDSKIGDADGMIQFMEADYDHLIEEASGISSINIPPFSPFETEEPP 180
           D  SE QL ELL ALDSKI  A+ MI+FMEADYDHLI++A G+ +    P S  E EE  
Sbjct: 121 DHQSEHQLMELLDALDSKIRVANDMIEFMEADYDHLIDQAIGMDT----PPSQTEDEETT 180

Query: 181 KLDIPESLIEPYGF-EYNVEKF---MEADYDHLIVEASEISSID 221
           + ++ +   EP  F EYNVE F   +E  +   +V+ + I   D
Sbjct: 181 QFNVSDLFNEPDEFEEYNVEGFQSLLEDKFLETLVQNNPIPDFD 211

[Close]

BLAST of Lsi05G003740 vs. TrEMBL
Match: A0A0A0LME6_CUCSA (Uncharacterized protein OS=Cucumis sativus GN=Csa_2G336070 PE=4 SV=1)

HSP 1 Score: 125.9 bits (315), Expect = 8.6e-26
Identity = 79/173 (45.66%), Postives = 110/173 (63.58%), Query Frame = 1


Query: 1   MGRGILSLKLIPNLKSRRTTFLKRKKSLMKKAYELSTLCDVQTCVFI----ASDCDPSTQ 60
           MGRG LS+KLI N KSR+TTF KRK SL++KAYELSTLCDV+ CV +     S+     Q
Sbjct: 1   MGRGKLSMKLISNEKSRKTTFHKRKASLLRKAYELSTLCDVRVCVVVHGPNQSNDQSPLQ 60

Query: 61  FETWPQNHDQIQAMIRSYKSHSFPKQSRNSSYDLQQFFSDRKNKIVTETSKLRQNVDKFK 120
             TWP + D++  MI SYK++   K+ R  S+ L  FFS+RK K+ T+ SKLR++V + +
Sbjct: 61  LHTWPPSADEVNNMIASYKTNCLYKRVR-KSFGLIDFFSERKKKVETDMSKLRKDVSEER 120

Query: 121 ----DPHLDSLSEQQLKELLAALDSKIGDADGMIQFMEADYDHLIEEASGISS 166
               D  LD L E QL+ L+  LDSKI  A   I+ +  + ++ +EE + + S
Sbjct: 121 FPSWDERLDHLLEDQLRVLMVELDSKIEIAKKRIE-IATENNYNVEEGTSVES 171

[Close]

BLAST of Lsi05G003740 vs. TrEMBL
Match: M5X3X5_PRUPE (Uncharacterized protein OS=Prunus persica GN=PRUPE_ppa021970mg PE=4 SV=1)

HSP 1 Score: 107.8 bits (268), Expect = 2.4e-20
Identity = 60/145 (41.38%), Postives = 89/145 (61.38%), Query Frame = 1


Query: 1   MGRGILSLKLIPNLKSRRTTFLKRKKSLMKKAYELSTLCDVQTCVFIASD--CDPSTQFE 60
           MGRG L ++LI N +SR+TTF KR K +MKKAYE STLC+V  C+ I      D   + +
Sbjct: 1   MGRGRLKMELIANERSRKTTFQKRSKGMMKKAYEFSTLCEVDVCIIIYGPKLTDRPPELQ 60

Query: 61  TWPQNHDQIQAMIRSYKSHSFPKQSRNSSYDLQQFFSDRKNKIVTETSKLRQNVDKFK-- 120
           TWPQN +Q+  +I  YK+ +  K ++  ++DL     DRKNK+  +  + R+ + + K  
Sbjct: 61  TWPQNSEQVDRIINKYKASTMSKPAK-KTFDLSDLLMDRKNKVYADIYRARKEMYEAKYP 120

Query: 121 --DPHLDSLSEQQLKELLAALDSKI 140
             D  ++S SE QL+ LL  LD+K+
Sbjct: 121 TWDERIESFSENQLEALLDKLDAKL 144

[Close]

BLAST of Lsi05G003740 vs. TrEMBL
Match: M5XI05_PRUPE (Uncharacterized protein (Fragment) OS=Prunus persica GN=PRUPE_ppa027181mg PE=4 SV=1)

HSP 1 Score: 101.7 bits (252), Expect = 1.7e-18
Identity = 56/145 (38.62%), Postives = 87/145 (60.00%), Query Frame = 1


Query: 1   MGRGILSLKLIPNLKSRRTTFLKRKKSLMKKAYELSTLCDVQTCVFI--ASDCDPSTQFE 60
           MGRG L ++LI N ++R+TTF KR+K +MKKAYE STLC++  C+ I      D   +  
Sbjct: 1   MGRGRLKMELIANERARKTTFQKRRKGMMKKAYEFSTLCELDVCMIIYGPKQTDRPPELH 60

Query: 61  TWPQNHDQIQAMIRSYKSHSFPKQSRNSSYDLQQFFSDRKNKIVTETSKLRQNVDKFK-- 120
           TWP+N D++  +I  YK+ S  ++    ++DL     DRK K+  +  + R+ + + K  
Sbjct: 61  TWPENRDEVDRIINKYKA-SIMRKPAKKTFDLSDLLRDRKTKVHVDIYRARKEMYEAKYP 120

Query: 121 --DPHLDSLSEQQLKELLAALDSKI 140
             D  ++S SE QL+ LL  LD+K+
Sbjct: 121 TWDERIESFSENQLEALLNTLDTKL 144

[Close]

BLAST of Lsi05G003740 vs. TrEMBL
Match: A0A059BT66_EUCGR (Uncharacterized protein OS=Eucalyptus grandis GN=EUGRSUZ_F02794 PE=4 SV=1)

HSP 1 Score: 100.1 bits (248), Expect = 5.0e-18
Identity = 67/173 (38.73%), Postives = 101/173 (58.38%), Query Frame = 1


Query: 1   MGRGILSLKLIPNLKSRRTTFLKRKKSLMKKAYELSTLCDVQTCVF---IASDCDPSTQF 60
           MGR  L LKLI N +SRRTTF KRKK LMKKA E  TLC V TCV    I    D   + 
Sbjct: 1   MGRAKLKLKLISNSRSRRTTFEKRKKGLMKKAGEFKTLCGVDTCVIINPIGISSDGLVKM 60

Query: 61  ETWPQNHDQIQAMIRSYKSHSFPKQSRNSSYDLQQFFSDRKNKIVTETSKLRQNVDKFKD 120
           ETWP +  +++ ++R Y+S +  +++R+++  L +FF+ RK KI  E +K  +   + K 
Sbjct: 61  ETWPPDRKEVERIVRRYQSEATDRRARHAT-GLHEFFASRKKKIDAELAKAHRANWEAKY 120

Query: 121 PHLD----SLSEQQLKELLAALDSKIGDADGMIQFMEADYDHLIEEASGISSI 167
           P  +     LS+ QL+ LL+A+D ++  A   +  M+      +E+A+  SS+
Sbjct: 121 PVSEEIFKDLSDPQLRSLLSAVDDRLEQAKTRLATMKE--KRRVEKAAPNSSV 170

[Close]

BLAST of Lsi05G003740 vs. TAIR10
Match: AT5G58890.1 (AT5G58890.1 AGAMOUS-like 82)

HSP 1 Score: 73.6 bits (179), Expect = 2.6e-13
Identity = 66/260 (25.38%), Postives = 121/260 (46.54%), Query Frame = 1


Query: 5   ILSLKLIPNLKSRRTTFLKRKKSLMKKAYELSTLCDVQTCVFIASDCDPS----TQFETW 64
           ++ L+ I N K+R TT+ KRK SL KKA E STLC V+TC+ +      +    ++ E W
Sbjct: 5   VVDLQRIANDKTRITTYKKRKASLYKKAQEFSTLCGVETCLIVYGPTKATDVVISEPEIW 64

Query: 65  PQNHDQIQAMIRSYKSHSFPKQSRNSSYDLQQFFSD--RKNKIVTETSKLRQNVDKFKDP 124
           P++  +++A+IR YK       S     +++ F +D  + N++VT+    R+N     + 
Sbjct: 65  PKDETKVRAIIRKYK--DTVSTSCRKETNVETFVNDVGKGNEVVTKKRVKRENKYSSWEE 124

Query: 125 HLDSLSEQQLKELLAALDSKIGDADGMIQFMEADYDHLIEEASGISSINIPPFSPFETEE 184
            LD  S +QL  +  A+DSK+ +A    +      +H   +     ++    F P    E
Sbjct: 125 KLDKCSREQLHGIFCAVDSKLNEAVTRQERSMFRVNHQAMDTPFPQNLMDQQFMPQYFHE 184

Query: 185 PPKL--------DIPESLIEPYGFEYNVEKFMEADYDHLIVEASEISSIDPPSSSSFETK 244
            P+         ++  SLI P+  +  ++  +   +  L +  S + S     +   + +
Sbjct: 185 QPQFQGFPNNFNNMGFSLISPHDGQIQMDPNLMEKWTDLALTQSLMMSKGNDGTQFMQRQ 244

Query: 245 EPPELDISELLIWPDGFEYN 251
           E P  +  +++    GF  N
Sbjct: 245 EQPYYNREQVVSRSAGFNVN 262

[Close]

BLAST of Lsi05G003740 vs. TAIR10
Match: AT3G18650.1 (AT3G18650.1 AGAMOUS-like 103)

HSP 1 Score: 60.1 bits (144), Expect = 2.9e-09
Identity = 43/132 (32.58%), Postives = 72/132 (54.55%), Query Frame = 1


Query: 16  SRRTTFLKRKKSLMKKAYELSTLCDVQTCVFIASDCDPSTQFETWPQNHDQIQAMIRSYK 75
           SR T+ +KR++++ KKA ELS LCD+  CV        + + +TWP+  ++++A+ R Y 
Sbjct: 31  SRATSLIKRQQTVFKKAKELSILCDIDVCVICYGS---NGELKTWPEEREKVKAIARRYG 90

Query: 76  SHSFPKQSRNSSYDLQQFFSDRKNKIVTETSKLR----QNVDKFK----DPHLDSLSEQQ 135
             S  K+ R  S DL +F  ++ NK   E  + +    + V K K    DP  D+ S +Q
Sbjct: 91  ELSETKR-RKGSVDLHEFL-EKMNKDDPEKEEKKKIKVRRVPKVKYPVWDPRFDNYSVEQ 150

Query: 136 LKELLAALDSKI 140
           L  L+ +L+  +
Sbjct: 151 LMGLVQSLERNL 157

[Close]

BLAST of Lsi05G003740 vs. TAIR10
Match: AT5G55690.1 (AT5G55690.1 MADS-box transcription factor family protein)

HSP 1 Score: 57.8 bits (138), Expect = 1.5e-08
Identity = 50/187 (26.74%), Postives = 81/187 (43.32%), Query Frame = 1


Query: 1   MGRGILSLKLIPNLKSRRTTFLKRKKSLMKKAYELSTLCDVQTCVFIASDCDPSTQF--- 60
           MGR ++ +  I N K+R TT+ KRK  L KKA E STLC V TCV +        +    
Sbjct: 1   MGRKMVKMTRITNEKTRITTYKKRKACLYKKASEFSTLCGVDTCVIVYGPSRAGDEMVME 60

Query: 61  -ETWPQNHDQIQAMIRSYKSHSFPKQSRNSSYDLQQFFSDRKNKIVTETSKLRQNVDKFK 120
            E WP++  +++ ++  Y+  +    S   +Y +Q+       K+   T   +       
Sbjct: 61  PELWPKDGSKVREILTKYRDTA--SSSCTKTYTVQECLEKNNTKVEKPTIATKYPT---W 120

Query: 121 DPHLDSLSEQQLKELLAALDSKIGDADGMIQFMEADYDHLIEEASGISSINIPPFSPFET 180
           D  LD  S   L  +  A+++KI +A    Q    D      +  G+   N   F  ++ 
Sbjct: 121 DKKLDQCSLNDLYAVFMAVENKIQEATNRNQTF-PDTSCWSNDQLGLCGYNRQCFEQYQL 180

Query: 181 EEPPKLD 184
              P +D
Sbjct: 181 FPLPTMD 181

[Close]

BLAST of Lsi05G003740 vs. TAIR10
Match: AT4G09960.3 (AT4G09960.3 K-box region and MADS-box transcription factor family protein )

HSP 1 Score: 57.8 bits (138), Expect = 1.5e-08
Identity = 41/135 (30.37%), Postives = 70/135 (51.85%), Query Frame = 1


Query: 1   MGRGILSLKLIPNLKSRRTTFLKRKKSLMKKAYELSTLCDVQTCVFIASDCDPSTQFETW 60
           MGRG + +K I N  +R+ TF KR+  L+KKAYELS LCD +  + +      ST+   +
Sbjct: 27  MGRGKIEIKRIENSTNRQVTFCKRRNGLLKKAYELSVLCDAEVALIVF-----STRGRLY 86

Query: 61  PQNHDQIQAMIRSYKSHSFPKQSRNSSYDLQQFFSDRKNKIVTETSKLRQNVDKFKDPHL 120
              ++ I++ I  YK       + ++  ++   +  +      E++KLRQ +   ++ + 
Sbjct: 87  EYANNNIRSTIERYKKACSDSTNTSTVQEINAAYYQQ------ESAKLRQQIQTIQNSNR 146

Query: 121 ----DSLSEQQLKEL 132
               DSLS   +KEL
Sbjct: 147 NLMGDSLSSLSVKEL 150

[Close]

BLAST of Lsi05G003740 vs. TAIR10
Match: AT2G03710.1 (AT2G03710.1 K-box region and MADS-box transcription factor family protein )

HSP 1 Score: 57.0 bits (136), Expect = 2.5e-08
Identity = 46/134 (34.33%), Postives = 66/134 (49.25%), Query Frame = 1


Query: 1   MGRGILSLKLIPNLKSRRTTFLKRKKSLMKKAYELSTLCDVQTCVFIASDCDPSTQFETW 60
           MGRG + LK I N  +R+ TF KR+  L+KKAYELS LCD +  + I S+     +F + 
Sbjct: 1   MGRGKVELKRIENKINRQVTFAKRRNGLLKKAYELSVLCDAEIALLIFSNRGKLYEFCSS 60

Query: 61  PQNHDQIQAMIRSYKSHSFPKQSRN-SSYDLQQFFSDRKNKIVTETSKLRQNVDKFKDPH 120
           P     +   +  Y+ HS+     N S+ DLQ  + D          K R  + +    H
Sbjct: 61  PSG---MARTVDKYRKHSYATMDPNQSAKDLQDKYQDYLK------LKSRVEILQHSQRH 120

Query: 121 L--DSLSEQQLKEL 132
           L  + LSE  + EL
Sbjct: 121 LLGEELSEMDVNEL 125

[Close]

BLAST of Lsi05G003740 vs. NCBI nr
Match: gi|449455824|ref|XP_004145650.1| (PREDICTED: agamous-like MADS-box protein AGL3 [Cucumis sativus])

HSP 1 Score: 238.4 bits (607), Expect = 1.7e-59
Identity = 141/224 (62.95%), Postives = 161/224 (71.88%), Query Frame = 1


Query: 1   MGRGILSLKLIPNLKSRRTTFLKRKKSLMKKAYELSTLCDVQTCVFIASDCDPSTQFETW 60
           MGRGILSLKLIPN KSRRTTFLKRKKSL+KKAYELSTLCDVQTC+FIASDCDPST FETW
Sbjct: 1   MGRGILSLKLIPNPKSRRTTFLKRKKSLIKKAYELSTLCDVQTCLFIASDCDPSTHFETW 60

Query: 61  PQNHDQIQAMIRSYKSHSFPKQSRNSSYDLQQFFSDRKNKIVTETSKLRQNVDKFKDPHL 120
           P NH QI  MIRSYKSHSF K   NSSYDL +FFSDRKNKI+T TSKL  NV       +
Sbjct: 61  PPNHHQIHQMIRSYKSHSFTKP--NSSYDLNRFFSDRKNKILTNTSKLLHNV-------V 120

Query: 121 DSLSEQQLKELLAALDSKIGDADGMIQFMEADYDHLIEEASGISSINIPPFSPFETEEPP 180
           D  SE QL ELL ALDSKI  A+ MI+FMEADYDHLI++A G+ +    P S  E EE  
Sbjct: 121 DHQSEHQLMELLDALDSKIRVANDMIEFMEADYDHLIDQAIGMDT----PPSQTEDEETT 180

Query: 181 KLDIPESLIEPYGF-EYNVEKF---MEADYDHLIVEASEISSID 221
           + ++ +   EP  F EYNVE F   +E  +   +V+ + I   D
Sbjct: 181 QFNVSDLFNEPDEFEEYNVEGFQSLLEDKFLETLVQNNPIPDFD 211

[Close]

BLAST of Lsi05G003740 vs. NCBI nr
Match: gi|659121915|ref|XP_008460878.1| (PREDICTED: MADS-box protein SOC1-like [Cucumis melo])

HSP 1 Score: 127.5 bits (319), Expect = 4.2e-26
Identity = 80/173 (46.24%), Postives = 110/173 (63.58%), Query Frame = 1


Query: 1   MGRGILSLKLIPNLKSRRTTFLKRKKSLMKKAYELSTLCDVQTCVFI----ASDCDPSTQ 60
           MGRG LS+KLI N KSR+TTF KRK SL++KAYELSTLCDV+ CVF+     S+     Q
Sbjct: 1   MGRGKLSMKLISNEKSRKTTFHKRKASLLRKAYELSTLCDVRVCVFVHGPNQSNDQSPLQ 60

Query: 61  FETWPQNHDQIQAMIRSYKSHSFPKQSRNSSYDLQQFFSDRKNKIVTETSKLRQNVDKFK 120
             TWP   D++ +MI SYK++   K++R  ++ L  FFS+RK K+ T+ SKLR++V + +
Sbjct: 61  LHTWPPCPDEVNSMIASYKTNCLHKRAR-KAFGLIDFFSERKKKVETDMSKLRKDVAEAR 120

Query: 121 ----DPHLDSLSEQQLKELLAALDSKIGDADGMIQFMEADYDHLIEEASGISS 166
               D  LD L E QL+ L+  LDSKI  A   I+     Y+  +EE + + S
Sbjct: 121 FTKWDERLDHLLEDQLRVLMMELDSKIEIAKKRIEIETECYN--VEEGTSVES 170

[Close]

BLAST of Lsi05G003740 vs. NCBI nr
Match: gi|778674258|ref|XP_011650173.1| (PREDICTED: floral homeotic protein DEFICIENS-like [Cucumis sativus])

HSP 1 Score: 125.9 bits (315), Expect = 1.2e-25
Identity = 79/173 (45.66%), Postives = 110/173 (63.58%), Query Frame = 1


Query: 1   MGRGILSLKLIPNLKSRRTTFLKRKKSLMKKAYELSTLCDVQTCVFI----ASDCDPSTQ 60
           MGRG LS+KLI N KSR+TTF KRK SL++KAYELSTLCDV+ CV +     S+     Q
Sbjct: 1   MGRGKLSMKLISNEKSRKTTFHKRKASLLRKAYELSTLCDVRVCVVVHGPNQSNDQSPLQ 60

Query: 61  FETWPQNHDQIQAMIRSYKSHSFPKQSRNSSYDLQQFFSDRKNKIVTETSKLRQNVDKFK 120
             TWP + D++  MI SYK++   K+ R  S+ L  FFS+RK K+ T+ SKLR++V + +
Sbjct: 61  LHTWPPSADEVNNMIASYKTNCLYKRVR-KSFGLIDFFSERKKKVETDMSKLRKDVSEER 120

Query: 121 ----DPHLDSLSEQQLKELLAALDSKIGDADGMIQFMEADYDHLIEEASGISS 166
               D  LD L E QL+ L+  LDSKI  A   I+ +  + ++ +EE + + S
Sbjct: 121 FPSWDERLDHLLEDQLRVLMVELDSKIEIAKKRIE-IATENNYNVEEGTSVES 171

[Close]

BLAST of Lsi05G003740 vs. NCBI nr
Match: gi|596029901|ref|XP_007219420.1| (hypothetical protein PRUPE_ppa021970mg [Prunus persica])

HSP 1 Score: 107.8 bits (268), Expect = 3.5e-20
Identity = 60/145 (41.38%), Postives = 89/145 (61.38%), Query Frame = 1


Query: 1   MGRGILSLKLIPNLKSRRTTFLKRKKSLMKKAYELSTLCDVQTCVFIASD--CDPSTQFE 60
           MGRG L ++LI N +SR+TTF KR K +MKKAYE STLC+V  C+ I      D   + +
Sbjct: 1   MGRGRLKMELIANERSRKTTFQKRSKGMMKKAYEFSTLCEVDVCIIIYGPKLTDRPPELQ 60

Query: 61  TWPQNHDQIQAMIRSYKSHSFPKQSRNSSYDLQQFFSDRKNKIVTETSKLRQNVDKFK-- 120
           TWPQN +Q+  +I  YK+ +  K ++  ++DL     DRKNK+  +  + R+ + + K  
Sbjct: 61  TWPQNSEQVDRIINKYKASTMSKPAK-KTFDLSDLLMDRKNKVYADIYRARKEMYEAKYP 120

Query: 121 --DPHLDSLSEQQLKELLAALDSKI 140
             D  ++S SE QL+ LL  LD+K+
Sbjct: 121 TWDERIESFSENQLEALLDKLDAKL 144

[Close]

BLAST of Lsi05G003740 vs. NCBI nr
Match: gi|694433726|ref|XP_009344134.1| (PREDICTED: agamous-like MADS-box protein AGL14 [Pyrus x bretschneideri])

HSP 1 Score: 104.4 bits (259), Expect = 3.8e-19
Identity = 58/145 (40.00%), Postives = 92/145 (63.45%), Query Frame = 1


Query: 1   MGRGILSLKLIPNLKSRRTTFLKRKKSLMKKAYELSTLCDVQTCVFIASD--CDPSTQFE 60
           MGRG L+++LI N +SR+TTF KR+K +MKKAYE STLC+V  C+ I      D   + +
Sbjct: 1   MGRGRLNMELIANERSRKTTFQKRRKGIMKKAYEFSTLCEVDVCMIIYGPKLTDRPPELQ 60

Query: 61  TWPQNHDQIQAMIRSYKSHSFPKQSRNSSYDLQQFFSDRKNKIVTETSKLRQNVDKFK-- 120
           TWP+N +++  +I  YK+ +  K ++  ++DL     DRKNK+  +  + R+ + + K  
Sbjct: 61  TWPKNPEEVSRIINKYKASTMCKPAK-KTFDLSDLLMDRKNKVYADIYRKRKEMYETKYP 120

Query: 121 --DPHLDSLSEQQLKELLAALDSKI 140
             D  +++ SE QL+ LL ALD+K+
Sbjct: 121 AWDERINTFSESQLEALLNALDAKL 144

The following BLAST results are available for this feature:

BLAST of Lsi05G003740 vs. Swiss-Prot


Match Name	E-value	Identity	Description
AGL82_ARATH	4.5e-12	25.38	Agamous-like MADS-box protein AGL82 OS=Arabidopsis thaliana GN=AGL82 PE=1 SV=1	[more]
AG_PANGI	6.1e-09	29.30	Floral homeotic protein AGAMOUS OS=Panax ginseng GN=AG2 PE=2 SV=1	[more]
AG103_ARATH	5.2e-08	32.58	Agamous-like MADS-box protein AGL103 OS=Arabidopsis thaliana GN=AGL103 PE=1 SV=1	[more]
MAD13_ORYSJ	6.8e-08	30.28	MADS-box transcription factor 13 OS=Oryza sativa subsp. japonica GN=MADS13 PE=1 ...	[more]
AGL11_ARATH	2.6e-07	30.37	Agamous-like MADS-box protein AGL11 OS=Arabidopsis thaliana GN=AGL11 PE=1 SV=1	[more]

BLAST of Lsi05G003740 vs. TrEMBL


Match Name	E-value	Identity	Description
A0A0A0LAL9_CUCSA	1.2e-59	62.95	Uncharacterized protein OS=Cucumis sativus GN=Csa_3G258140 PE=4 SV=1	[more]
A0A0A0LME6_CUCSA	8.6e-26	45.66	Uncharacterized protein OS=Cucumis sativus GN=Csa_2G336070 PE=4 SV=1	[more]
M5X3X5_PRUPE	2.4e-20	41.38	Uncharacterized protein OS=Prunus persica GN=PRUPE_ppa021970mg PE=4 SV=1	[more]
M5XI05_PRUPE	1.7e-18	38.62	Uncharacterized protein (Fragment) OS=Prunus persica GN=PRUPE_ppa027181mg PE=4 S...	[more]
A0A059BT66_EUCGR	5.0e-18	38.73	Uncharacterized protein OS=Eucalyptus grandis GN=EUGRSUZ_F02794 PE=4 SV=1	[more]

BLAST of Lsi05G003740 vs. TAIR10


Match Name	E-value	Identity	Description
AT5G58890.1	2.6e-13	25.38	AGAMOUS-like 82	[more]
AT3G18650.1	2.9e-09	32.58	AGAMOUS-like 103	[more]
AT5G55690.1	1.5e-08	26.74	MADS-box transcription factor family protein	[more]
AT4G09960.3	1.5e-08	30.37	K-box region and MADS-box transcription factor family protein	[more]
AT2G03710.1	2.5e-08	34.33	K-box region and MADS-box transcription factor family protein	[more]

BLAST of Lsi05G003740 vs. NCBI nr


Match Name	E-value	Identity	Description
gi\|449455824\|ref\|XP_004145650.1\|	1.7e-59	62.95	PREDICTED: agamous-like MADS-box protein AGL3 [Cucumis sativus]	[more]
gi\|659121915\|ref\|XP_008460878.1\|	4.2e-26	46.24	PREDICTED: MADS-box protein SOC1-like [Cucumis melo]	[more]
gi\|778674258\|ref\|XP_011650173.1\|	1.2e-25	45.66	PREDICTED: floral homeotic protein DEFICIENS-like [Cucumis sativus]	[more]
gi\|596029901\|ref\|XP_007219420.1\|	3.5e-20	41.38	hypothetical protein PRUPE_ppa021970mg [Prunus persica]	[more]
gi\|694433726\|ref\|XP_009344134.1\|	3.8e-19	40.00	PREDICTED: agamous-like MADS-box protein AGL14 [Pyrus x bretschneideri]	[more]

Annotated Terms

The following terms have been associated with this gene:

Vocabulary: **Molecular Function**
Term	Definition
GO:0046983	protein dimerization activity
GO:0003677	DNA binding

Vocabulary: **INTERPRO**
Term	Definition
IPR002100	TF_MADSbox

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0006351	transcription, DNA-templated
biological_process	GO:0008150	biological_process
cellular_component	GO:0005575	cellular_component
molecular_function	GO:0003677	DNA binding
molecular_function	GO:0046983	protein dimerization activity

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Lsi05G003740.1	Lsi05G003740.1	mRNA

InterPro

Analysis Name: InterPro Annotations of Lagenaria siceraria
Date Performed: 2017-09-18


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR002100	Transcription factor, MADS-box	PRINTS	PR00404	MADSDOMAIN	coord: 3..23 score: 2.9E-11coord: 23..38 score: 2.9E-11coord: 38..59 score: 2.9
IPR002100	Transcription factor, MADS-box	PFAM	PF00319	SRF-TF	coord: 11..51 score: 2.2
IPR002100	Transcription factor, MADS-box	SMART	SM00432	madsneu2	coord: 1..60 score: 3.1
IPR002100	Transcription factor, MADS-box	PROFILE	PS50066	MADS_BOX_2	coord: 1..61 score: 1
IPR002100	Transcription factor, MADS-box	unknown	SSF55455	SRF-like	coord: 2..89 score: 2.22
None	No IPR available	PANTHER	PTHR11945	MADS BOX PROTEIN	coord: 2..167 score: 7.3
None	No IPR available	PANTHER	PTHR11945:SF176	PROTEIN AGAMOUS-LIKE 82	coord: 2..167 score: 7.3

Synteny

The following gene(s) are orthologous to this gene:


Gene	Orthologue	Organism	Block
Lsi05G003740	CmaCh14G008180	Cucurbita maxima (Rimu)	cmalsiB249
Lsi05G003740	CmoCh14G008310	Cucurbita moschata (Rifu)	cmolsiB237
Lsi05G003740	Cp4.1LG03g06160	Cucurbita pepo (Zucchini)	cpelsiB511
Lsi05G003740	Carg22264	Silver-seed gourd	carlsiB435

The following gene(s) are paralogous to this gene:

None

The following block(s) are covering this gene:


Gene	Organism	Block
Lsi05G003740	Wax gourd	lsiwgoB447
Lsi05G003740	Cucumber (Gy14) v1	cgylsiB004
Lsi05G003740	Cucurbita maxima (Rimu)	cmalsiB440
Lsi05G003740	Cucurbita moschata (Rifu)	cmolsiB430
Lsi05G003740	Cucurbita pepo (Zucchini)	cpelsiB470
Lsi05G003740	Wild cucumber (PI 183967)	cpilsiB210
Lsi05G003740	Cucumber (Chinese Long) v2	culsiB212
Lsi05G003740	Melon (DHL92) v3.5.1	lsimeB368
Lsi05G003740	Watermelon (Charleston Gray)	lsiwcgB332
Lsi05G003740	Watermelon (97103) v1	lsiwmB361
Lsi05G003740	Cucumber (Gy14) v2	cgyblsiB199
Lsi05G003740	Melon (DHL92) v3.6.1	lsimedB384
Lsi05G003740	Silver-seed gourd	carlsiB700
Lsi05G003740	Cucumber (Chinese Long) v3	cuclsiB231
Lsi05G003740	Watermelon (97103) v2	lsiwmbB329
Lsi05G003740	Watermelon (97103) v2	lsiwmbB340