Lsi05G000450 (gene) Bottle gourd (USVL1VR-Ls) | Cucurbit Genomics Database (CuGenDB)

Overview


Name	Lsi05G000450
Type	gene
Organism	Lagenaria siceraria (Bottle gourd (USVL1VR-Ls))
Description	Plant invertase/pectin methylesterase inhibitor superfamily protein, putative
Location	chr05 : 965787 .. 966338 (+)

Genome Viewer

Gene Expression

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: CDS

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGGAAAATCCCATTTCAAAATCAATTTCATTACTTCTTTTCATTCCAACATTTCTTCAATTAGCATATTGTTTAGACATTGTTCAACAGTCTTGCAAATTGGCTGCACAAACAGACCCATATGTTAACTACAAACTATGTGTTCAAAGCCTTAAAGCCAATCCCAATTATTCCAAAGCTGCAAATTTCAAAGACTTGCTTATGATTTCAATCAACCACTCAAAGGCCAATGCCACAGAAATTGGGTCACAGATTTCAGAGCTTTTGAAAGGAAGTAATGAGAAATTGGGGAAATATTCATTGAATTGTTTGAAAAGCTGTTTAGAACTTTATTCTGAGGCTATTTCTGATTTGGAAAAGGCTTTAGGAGCTTTGAAAATGGAGGATTATGAGACAGCAAATACAGGGGTTAGTGCAGCCATGGATGCACCAATAAGTTGTGAAGATGGGTATAGGGAAAAAGATGGAGAAGTTTCTCCTTTCACAGAGATCAATGATGGGTTCTTTCAATTGATTGCAATTTCTCTTGCTTTTATTAATATGTCTAAGTAA

mRNA sequence

ATGGAAAATCCCATTTCAAAATCAATTTCATTACTTCTTTTCATTCCAACATTTCTTCAATTAGCATATTGTTTAGACATTGTTCAACAGTCTTGCAAATTGGCTGCACAAACAGACCCATATGTTAACTACAAACTATGTGTTCAAAGCCTTAAAGCCAATCCCAATTATTCCAAAGCTGCAAATTTCAAAGACTTGCTTATGATTTCAATCAACCACTCAAAGGCCAATGCCACAGAAATTGGGTCACAGATTTCAGAGCTTTTGAAAGGAAGTAATGAGAAATTGGGGAAATATTCATTGAATTGTTTGAAAAGCTGTTTAGAACTTTATTCTGAGGCTATTTCTGATTTGGAAAAGGCTTTAGGAGCTTTGAAAATGGAGGATTATGAGACAGCAAATACAGGGGTTAGTGCAGCCATGGATGCACCAATAAGTTGTGAAGATGGGTATAGGGAAAAAGATGGAGAAGTTTCTCCTTTCACAGAGATCAATGATGGGTTCTTTCAATTGATTGCAATTTCTCTTGCTTTTATTAATATGTCTAAGTAA

Coding sequence (CDS)

ATGGAAAATCCCATTTCAAAATCAATTTCATTACTTCTTTTCATTCCAACATTTCTTCAATTAGCATATTGTTTAGACATTGTTCAACAGTCTTGCAAATTGGCTGCACAAACAGACCCATATGTTAACTACAAACTATGTGTTCAAAGCCTTAAAGCCAATCCCAATTATTCCAAAGCTGCAAATTTCAAAGACTTGCTTATGATTTCAATCAACCACTCAAAGGCCAATGCCACAGAAATTGGGTCACAGATTTCAGAGCTTTTGAAAGGAAGTAATGAGAAATTGGGGAAATATTCATTGAATTGTTTGAAAAGCTGTTTAGAACTTTATTCTGAGGCTATTTCTGATTTGGAAAAGGCTTTAGGAGCTTTGAAAATGGAGGATTATGAGACAGCAAATACAGGGGTTAGTGCAGCCATGGATGCACCAATAAGTTGTGAAGATGGGTATAGGGAAAAAGATGGAGAAGTTTCTCCTTTCACAGAGATCAATGATGGGTTCTTTCAATTGATTGCAATTTCTCTTGCTTTTATTAATATGTCTAAGTAA

Protein sequence

MENPISKSISLLLFIPTFLQLAYCLDIVQQSCKLAAQTDPYVNYKLCVQSLKANPNYSKAANFKDLLMISINHSKANATEIGSQISELLKGSNEKLGKYSLNCLKSCLELYSEAISDLEKALGALKMEDYETANTGVSAAMDAPISCEDGYREKDGEVSPFTEINDGFFQLIAISLAFINMSK

Homology

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BLAST of Lsi05G000450 vs. Swiss-Prot
Match: PLA1_PLAAC (Putative invertase inhibitor OS=Platanus acerifolia PE=1 SV=1)

HSP 1 Score: 110.9 bits (276), Expect = 1.5e-23
Identity = 71/177 (40.11%), Postives = 104/177 (58.76%), Query Frame = 1


Query: 8   SISLLLF---IPTFLQLAYCLDIVQQSCKLAAQTDPYVNYKLCVQSLKANPNYSKAANFK 67
           S SL +F   +   LQ     DIVQ +CK  AQ  P VNY  CV+SL A+P  S  A+ +
Sbjct: 4   SFSLCIFFFNLLLLLQAVISADIVQGTCKKVAQRSPNVNYDFCVKSLGADPK-SHTADLQ 63

Query: 68  DLLMISINHSKANATEIGSQISELLKGSNEKLGKYSLNCLKSCLELYSEAISDLEKALGA 127
            L +IS N +  + ++I + I  +LK   +   K  LN    C+ LY++A S +++A+  
Sbjct: 64  GLGVISANLAIQHGSKIQTFIGRILKSKVDPALKKYLN---DCVGLYADAKSSVQEAIAD 123

Query: 128 LKMEDYETANTGVSAAMDAPISCEDGYREKDGEVSPFTEINDGFFQLIAISLAFINM 182
            K +DY +AN  +SAA+D  ++CEDG++EK G VSP T+ N  + QL AISLA   +
Sbjct: 124 FKSKDYASANVKMSAALDDSVTCEDGFKEKKGIVSPVTKENKDYVQLTAISLAITKL 176

[Close]

BLAST of Lsi05G000450 vs. TrEMBL
Match: A0A0A0LHE9_CUCSA (Uncharacterized protein OS=Cucumis sativus GN=Csa_3G910710 PE=4 SV=1)

HSP 1 Score: 283.9 bits (725), Expect = 1.4e-73
Identity = 145/181 (80.11%), Postives = 163/181 (90.06%), Query Frame = 1


Query: 1   MENPISKSISLLLFIPTFLQLAYCLDIVQQSCKLAAQTDPYVNYKLCVQSLKANPNYSKA 60
           MENP S+SI  LL +PT +QLAY LDIVQ SCKLAA+TDPYV+YKLCVQ+LKA+PN SK 
Sbjct: 1   MENPTSRSILFLLCVPTLIQLAYGLDIVQHSCKLAAKTDPYVDYKLCVQTLKASPN-SKD 60

Query: 61  ANFKDLLMISINHSKANATEIGSQISELLKGSNEKLGKYSLNCLKSCLELYSEAISDLEK 120
           A FKDL++ISIN SKANATEIGS+ISEL+K  +EK G+YSLNCLKSCLELYSEA+SDLEK
Sbjct: 61  AEFKDLVVISINQSKANATEIGSEISELMKRRSEKWGQYSLNCLKSCLELYSEAVSDLEK 120

Query: 121 ALGALKMEDYETANTGVSAAMDAPISCEDGYREKDGEVSPFTEINDGFFQLIAISLAFIN 180
           AL  LKMEDYETA TGVSAAMDAP+SCEDGY+EKDGEVSP +EINDGFFQL+AISLAFIN
Sbjct: 121 ALKGLKMEDYETAKTGVSAAMDAPVSCEDGYKEKDGEVSPLSEINDGFFQLVAISLAFIN 180

Query: 181 M 182
           M
Sbjct: 181 M 180

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BLAST of Lsi05G000450 vs. TrEMBL
Match: A0A059C0T9_EUCGR (Uncharacterized protein OS=Eucalyptus grandis GN=EUGRSUZ_E00321 PE=4 SV=1)

HSP 1 Score: 141.4 bits (355), Expect = 1.1e-30
Identity = 79/156 (50.64%), Postives = 108/156 (69.23%), Query Frame = 1


Query: 26  DIVQQSCKLAAQTDPYVNYKLCVQSLKANPNYSKAANFKDLLMISINHSKANATEIGSQI 85
           +IV  SCK AA  DP ++Y+ CV   +A+   S+ AN +DL +ISIN + +NAT++ S I
Sbjct: 26  NIVHGSCKRAADGDPNISYEFCVACFEAD-RMSQPANVEDLALISINLTISNATKVSSCI 85

Query: 86  SELLKGSNEKLGKYSLNCLKSCLELYSEAISDLEKALGALKMEDYETANTGVSAAMDAPI 145
           S+LL+  N+ L  ++ +CL +CLELYS+A S L   +  L   DY  AN  VSAAMDA +
Sbjct: 86  SKLLQ--NKTLDHFARDCLTTCLELYSDAYSTLRGVVDDLGSRDYYKANVDVSAAMDAAV 145

Query: 146 SCEDGYREKDGEVSPFTEINDGFFQLIAISLAFINM 182
           +CEDG++E++G VSP T+ ND FFQL AISLAFI M
Sbjct: 146 TCEDGFKEREGLVSPLTKENDMFFQLNAISLAFIKM 178

[Close]

BLAST of Lsi05G000450 vs. TrEMBL
Match: A0A061G2M1_THECC (Plant invertase/pectin methylesterase inhibitor superfamily protein, putative OS=Theobroma cacao GN=TCM_015798 PE=4 SV=1)

HSP 1 Score: 140.2 bits (352), Expect = 2.5e-30
Identity = 79/181 (43.65%), Postives = 115/181 (63.54%), Query Frame = 1


Query: 1   MENPISKSISLLLFIPTFLQLAYCLDIVQQSCKLAAQTDPYVNYKLCVQSLKANPNYSKA 60
           MEN      + + F    L ++   D++Q SC  AA+ D  +N+  CV SL+ NP    A
Sbjct: 74  MENSFPSVCTFIAFF-FLLLVSASSDVIQDSCDKAAKGDRNINFDFCVASLEGNPKSKTA 133

Query: 61  ANFKDLLMISINHSKANATEIGSQISELLKGSNEKLGKYSLNCLKSCLELYSEAISDLEK 120
            + + L+ ISI  + +NAT I S IS+LLK  N+ L   + +CL+ C ELYS+A  +LE 
Sbjct: 134 TSTESLVPISIEMAISNATSISSIISKLLK--NKNLDNPTRSCLEDCSELYSDAQPNLEI 193

Query: 121 ALGALKMEDYETANTGVSAAMDAPISCEDGYREKDGEVSPFTEINDGFFQLIAISLAFIN 180
              A++ +DY TA T +SAA+DAP++CEDG++E++G VSP T+ ND FFQL  + LAFIN
Sbjct: 194 GGQAVESKDYGTAKTVISAALDAPVTCEDGFKEREGLVSPLTKENDEFFQLTVVPLAFIN 251

Query: 181 M 182
           +
Sbjct: 254 L 251

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BLAST of Lsi05G000450 vs. TrEMBL
Match: M5WAV9_PRUPE (Uncharacterized protein OS=Prunus persica GN=PRUPE_ppa012101mg PE=4 SV=1)

HSP 1 Score: 139.4 bits (350), Expect = 4.3e-30
Identity = 77/155 (49.68%), Postives = 109/155 (70.32%), Query Frame = 1


Query: 26  DIVQQSCKLAAQTDPYVNYKLCVQSLKANPNYSKAANFKDLLMISINHSKANATEIGSQI 85
           D++Q SCK A+Q DP ++YK CV SL+AN     + + ++L++IS+N + ANAT I S I
Sbjct: 27  DLIQLSCKKASQGDPNLSYKFCVSSLEANAK-GHSLDLQELVVISLNLTIANATNINSTI 86

Query: 86  SELLKGSNEKLGKYSLNCLKSCLELYSEAISDLEKALGALKMEDYETANTGVSAAMDAPI 145
           S+LLK  ++    ++   L+ C ELY++AI  L++AL A + +D+  AN  VS+AMDA  
Sbjct: 87  SKLLK--DKAFDNFAKERLRGCSELYADAIPTLQEALCAFQSKDFAKANIEVSSAMDASS 146

Query: 146 SCEDGYREKDGEVSPFTEINDGFFQLIAISLAFIN 181
           +CEDG++EK GEVSP  + ND FFQL AISLAFIN
Sbjct: 147 TCEDGFKEKKGEVSPLRKENDVFFQLNAISLAFIN 178

[Close]

BLAST of Lsi05G000450 vs. TrEMBL
Match: A0A0D2PIZ3_GOSRA (Uncharacterized protein OS=Gossypium raimondii GN=B456_001G141900 PE=4 SV=1)

HSP 1 Score: 136.3 bits (342), Expect = 3.6e-29
Identity = 84/175 (48.00%), Postives = 112/175 (64.00%), Query Frame = 1


Query: 9   ISLLLFIPTFLQLAYCLDIVQQSCKLAAQTDPYVNYKL--CVQSLKANPNYSKAANFKDL 68
           IS LLF    L  A   D++Q+SC  A++ +P  N KL  CV   + NP    A    DL
Sbjct: 6   ISCLLFFYLLLVSATS-DLIQKSCYEASKGNP-ANVKLDFCVSGFQGNPKAKAAYGVADL 65

Query: 69  LMISINHSKANATEIGSQISELLKGSNEKLGKYSLNCLKSCLELYSEAISDLEKALGALK 128
           +++SI  + ANAT IGS+IS+LL   N+ +G ++ NCLK C ELYS A S LE  L A +
Sbjct: 66  VLVSIETAIANATAIGSKISKLL--DNKSVGMFARNCLKDCSELYSLAGSSLETGLDAFQ 125

Query: 129 MEDYETANTGVSAAMDAPISCEDGYREKDGEVSPFTEINDGFFQLIAISLAFINM 182
             DY TAN  +SAA+DAP++CED ++EK+G VSP T+ N+ F QL AI LAF+ M
Sbjct: 126 AVDYGTANAEISAALDAPVTCEDQFKEKNGLVSPLTKENNNFRQLTAIPLAFMKM 176

[Close]

BLAST of Lsi05G000450 vs. TAIR10
Match: AT5G46940.1 (AT5G46940.1 Plant invertase/pectin methylesterase inhibitor superfamily protein)

HSP 1 Score: 118.6 bits (296), Expect = 4.0e-27
Identity = 68/179 (37.99%), Postives = 103/179 (57.54%), Query Frame = 1


Query: 9   ISLLLFIPTFLQLAYCL----DIVQQSCKLAAQTDPYVNYKLCVQSLKANPNYSKAANFK 68
           +  LL++ TF  L+  L     +++ SCK A  T P   Y LCV SL+ NP    A +  
Sbjct: 1   MKFLLYLVTFFVLSNGLANGQTLIRNSCKKATATSPKFKYNLCVTSLETNPQAKTAKDLA 60

Query: 69  DLLMISINHSKANATEIGSQISELLKGSNEKLGKYSLNCLKSCLELYSEAISDLEKALGA 128
            L+M S  ++   AT +   + +++KG  +K+ K +   L+ CL+LY++AI  L +AL  
Sbjct: 61  GLVMASTKNAVTKATTLKGTVDKIIKG--KKVNKMTAMPLRDCLQLYTDAIGSLNEALAG 120

Query: 129 LKMEDYETANTGVSAAMDAPISCEDGYREKDGEVSPFTEINDGFFQLIAISLAFINMSK 184
           +K  +Y T  T +SAAMD P +CE G++E+    SP T+ ND  +Q+I I LAF NM K
Sbjct: 121 VKSRNYPTVKTVLSAAMDTPSTCETGFKERKAP-SPVTKENDNLYQMILIPLAFTNMLK 176

[Close]

BLAST of Lsi05G000450 vs. TAIR10
Match: AT5G46930.1 (AT5G46930.1 Plant invertase/pectin methylesterase inhibitor superfamily protein)

HSP 1 Score: 104.8 bits (260), Expect = 5.9e-23
Identity = 61/175 (34.86%), Postives = 98/175 (56.00%), Query Frame = 1


Query: 8   SISLLLFIPTFLQLAYCLDIVQQSCKLAAQTDPYVNYKLCVQSLKANPNYSKAANFKDLL 67
           S+ L++F       A    +++ SCK A   DP + Y  CV+SL+ NP    A + + L+
Sbjct: 4   SLYLVMFFLLLNCFATAQSLIRDSCKKATTKDPKLKYDFCVKSLEENPQSKTAKSLEGLV 63

Query: 68  MISINHSKANATEIGSQISELLKGSNEKLGKYSLNCLKSCLELYSEAISDLEKALGALKM 127
            +S  ++ +  T +   + ++LK    ++ +  L+    CLELY+EAI  L ++L  +K 
Sbjct: 64  FVSTKNAVSKTTSLKGMVDKILKEDKYEVERPLLD----CLELYTEAIDSLNQSLDTVKS 123

Query: 128 EDYETANTGVSAAMDAPISCEDGY-REKDGEVSPFTEINDGFFQLIAISLAFINM 182
            DY+TA   +SAAMDAP SCE  + + K    SPFT+ ND  F ++ I +AF +M
Sbjct: 124 RDYKTATMLMSAAMDAPGSCETKFTKRKKAVKSPFTKENDVLFYMVLIPIAFTSM 174

[Close]

BLAST of Lsi05G000450 vs. TAIR10
Match: AT5G46970.1 (AT5G46970.1 Plant invertase/pectin methylesterase inhibitor superfamily protein)

HSP 1 Score: 104.0 bits (258), Expect = 1.0e-22
Identity = 58/144 (40.28%), Postives = 89/144 (61.81%), Query Frame = 1


Query: 27  IVQQSCKLAAQTDPYVNYKLCVQSLKANPNYSKAANFKDLLMISINHSKANATEIGSQIS 86
           ++Q  CK AA  +P ++Y  CV+SL+ NP    A +   L+M+S  ++ +  T +   + 
Sbjct: 23  LIQDFCKKAADKNPKIHYNFCVKSLEENPQSKTARSLDRLVMLSTKNAVSKTTSMKGIVD 82

Query: 87  ELLKGSNEKLGKYSLNCLKSCLELYSEAISDLEKALGALKMEDYETANTGVSAAMDAPIS 146
           ++LK +  ++  YS   L+ CLELYS+A + L++AL  +K  DY+TAN  +SAAM AP S
Sbjct: 83  KILKENRFEM--YSEKPLRDCLELYSDATNSLKEALTIIKSRDYKTANVVISAAMGAPPS 142

Query: 147 CEDGYRE-KDGEVSPFTEINDGFF 170
           CE G++E K    SPFT+ ND  F
Sbjct: 143 CEIGFKEGKKPLKSPFTKDNDVLF 164

[Close]

BLAST of Lsi05G000450 vs. TAIR10
Match: AT5G46960.1 (AT5G46960.1 Plant invertase/pectin methylesterase inhibitor superfamily protein)

HSP 1 Score: 94.4 bits (233), Expect = 8.0e-20
Identity = 60/173 (34.68%), Postives = 91/173 (52.60%), Query Frame = 1


Query: 9   ISLLLFIPTFLQLAYCLDIVQQSCKLAAQTDPYVNYKLCVQSLKANPNYSKAANFKDLLM 68
           +SL++F       A    ++Q SCK A   DP ++Y  CV SL  +P    A   + L++
Sbjct: 5   VSLVIFSLFLNGFATAQTLIQDSCKKAFAKDPQLSYDFCVNSLTQDPQSKAATTLESLVL 64

Query: 69  ISINHSKANATEIGSQISELLKGSNEKLGKYSLNCLKSCLELYSEAISDLEKALGALKME 128
            S   + A  T +   +++ LK   ++  +  +  LK CL  Y++A  DL  AL  +K  
Sbjct: 65  ASTKTAAAKITNLKGIVAQDLK---DQRYQDIVEDLKLCLGFYNDANDDLTTALANIKSR 124

Query: 129 DYETANTGVSAAMDAPISCEDGYREKDGEVSPFTEINDGFFQLIAISLAFINM 182
           DY+ AN  +SAA+D P +CED ++E   + SP T  N   F+ I I LAF NM
Sbjct: 125 DYQGANINLSAALDVPGNCEDDFKEAK-KTSPITNENSILFKTILIPLAFTNM 173

[Close]

BLAST of Lsi05G000450 vs. TAIR10
Match: AT5G46950.1 (AT5G46950.1 Plant invertase/pectin methylesterase inhibitor superfamily protein)

HSP 1 Score: 92.4 bits (228), Expect = 3.0e-19
Identity = 59/173 (34.10%), Postives = 92/173 (53.18%), Query Frame = 1


Query: 9   ISLLLFIPTFLQLAYCLDIVQQSCKLAAQTDPYVNYKLCVQSLKANPNYSKAANFKDLLM 68
           +SL++F       A    ++Q SCK A   DP  +Y  CVQSL  +P    A   +DL +
Sbjct: 5   VSLVIFSLFLNGFATAQTLIQDSCKKAFAKDPQSSYDFCVQSLTQDPQSKAATTLEDLAL 64

Query: 69  ISINHSKANATEIGSQISELLKGSNEKLGKYSLNCLKSCLELYSEAISDLEKALGALKME 128
            S  +  A  T +   +++ LK   ++  +  +  LK CL  Y +A   L+ AL  +K  
Sbjct: 65  ASTKNVAAKITNLKGIVAQDLK---DQRYQDIVEDLKLCLGFYKDANDSLKTALANIKSR 124

Query: 129 DYETANTGVSAAMDAPISCEDGYREKDGEVSPFTEINDGFFQLIAISLAFINM 182
           DY+ AN+ +SAA++AP  CED ++E + + SP T  N+  ++ I I LAF  M
Sbjct: 125 DYDGANSNLSAALNAPGDCEDDFKEAE-KKSPITNENNILYKTIVIPLAFTTM 173

[Close]

BLAST of Lsi05G000450 vs. NCBI nr
Match: gi|449461581|ref|XP_004148520.1| (PREDICTED: putative invertase inhibitor [Cucumis sativus])

HSP 1 Score: 283.9 bits (725), Expect = 2.0e-73
Identity = 145/181 (80.11%), Postives = 163/181 (90.06%), Query Frame = 1


Query: 1   MENPISKSISLLLFIPTFLQLAYCLDIVQQSCKLAAQTDPYVNYKLCVQSLKANPNYSKA 60
           MENP S+SI  LL +PT +QLAY LDIVQ SCKLAA+TDPYV+YKLCVQ+LKA+PN SK 
Sbjct: 1   MENPTSRSILFLLCVPTLIQLAYGLDIVQHSCKLAAKTDPYVDYKLCVQTLKASPN-SKD 60

Query: 61  ANFKDLLMISINHSKANATEIGSQISELLKGSNEKLGKYSLNCLKSCLELYSEAISDLEK 120
           A FKDL++ISIN SKANATEIGS+ISEL+K  +EK G+YSLNCLKSCLELYSEA+SDLEK
Sbjct: 61  AEFKDLVVISINQSKANATEIGSEISELMKRRSEKWGQYSLNCLKSCLELYSEAVSDLEK 120

Query: 121 ALGALKMEDYETANTGVSAAMDAPISCEDGYREKDGEVSPFTEINDGFFQLIAISLAFIN 180
           AL  LKMEDYETA TGVSAAMDAP+SCEDGY+EKDGEVSP +EINDGFFQL+AISLAFIN
Sbjct: 121 ALKGLKMEDYETAKTGVSAAMDAPVSCEDGYKEKDGEVSPLSEINDGFFQLVAISLAFIN 180

Query: 181 M 182
           M
Sbjct: 181 M 180

[Close]

BLAST of Lsi05G000450 vs. NCBI nr
Match: gi|659131910|ref|XP_008465919.1| (PREDICTED: putative invertase inhibitor [Cucumis melo])

HSP 1 Score: 274.6 bits (701), Expect = 1.2e-70
Identity = 141/181 (77.90%), Postives = 161/181 (88.95%), Query Frame = 1


Query: 1   MENPISKSISLLLFIPTFLQLAYCLDIVQQSCKLAAQTDPYVNYKLCVQSLKANPNYSKA 60
           MENPI+KSI  LL +PT +QLA  LDIVQ  CKLAA+TDPYV+YKLCVQ+LKANPN S+ 
Sbjct: 1   MENPIAKSILFLLCVPTLIQLANGLDIVQHYCKLAAKTDPYVDYKLCVQTLKANPN-SED 60

Query: 61  ANFKDLLMISINHSKANATEIGSQISELLKGSNEKLGKYSLNCLKSCLELYSEAISDLEK 120
           A+FKDL+MISIN +KANATEI S+ISEL+K ++EK GKYSLNCLKSCLELYSEA+SDL+K
Sbjct: 61  ADFKDLVMISINQAKANATEIRSEISELMKRTSEKWGKYSLNCLKSCLELYSEAVSDLKK 120

Query: 121 ALGALKMEDYETANTGVSAAMDAPISCEDGYREKDGEVSPFTEINDGFFQLIAISLAFIN 180
           AL  LKMEDYETA T VSAAMDAP+SCEDGY+EKDGEVSP +E NDGFFQL+AISLAFIN
Sbjct: 121 ALRGLKMEDYETAKTAVSAAMDAPVSCEDGYKEKDGEVSPLSETNDGFFQLVAISLAFIN 180

Query: 181 M 182
           M
Sbjct: 181 M 180

[Close]

BLAST of Lsi05G000450 vs. NCBI nr
Match: gi|645246780|ref|XP_008229513.1| (PREDICTED: putative invertase inhibitor [Prunus mume])

HSP 1 Score: 146.4 bits (368), Expect = 5.0e-32
Identity = 79/155 (50.97%), Postives = 111/155 (71.61%), Query Frame = 1


Query: 26  DIVQQSCKLAAQTDPYVNYKLCVQSLKANPNYSKAANFKDLLMISINHSKANATEIGSQI 85
           D++Q SCK A+Q DP ++YK CV SL+AN     + + ++L++IS+N + ANAT I S I
Sbjct: 27  DLIQLSCKKASQGDPNLSYKFCVSSLEANAK-GHSLDLQELVVISLNLTVANATNINSTI 86

Query: 86  SELLKGSNEKLGKYSLNCLKSCLELYSEAISDLEKALGALKMEDYETANTGVSAAMDAPI 145
           S+LLK  ++   K++  CL+ C ELY++AI  L++AL A + +D+  AN  VS+AMDA  
Sbjct: 87  SKLLK--DKAFDKFAKECLRGCSELYADAIPTLQEALCAFQSKDFAKANIEVSSAMDASS 146

Query: 146 SCEDGYREKDGEVSPFTEINDGFFQLIAISLAFIN 181
           +CEDG++EK GEVSP  + ND FFQL AISLAFIN
Sbjct: 147 TCEDGFKEKKGEVSPLRKENDVFFQLNAISLAFIN 178

[Close]

BLAST of Lsi05G000450 vs. NCBI nr
Match: gi|702353236|ref|XP_010058175.1| (PREDICTED: putative invertase inhibitor [Eucalyptus grandis])

HSP 1 Score: 141.4 bits (355), Expect = 1.6e-30
Identity = 79/156 (50.64%), Postives = 108/156 (69.23%), Query Frame = 1


Query: 26  DIVQQSCKLAAQTDPYVNYKLCVQSLKANPNYSKAANFKDLLMISINHSKANATEIGSQI 85
           +IV  SCK AA  DP ++Y+ CV   +A+   S+ AN +DL +ISIN + +NAT++ S I
Sbjct: 26  NIVHGSCKRAADGDPNISYEFCVACFEAD-RMSQPANVEDLALISINLTISNATKVSSCI 85

Query: 86  SELLKGSNEKLGKYSLNCLKSCLELYSEAISDLEKALGALKMEDYETANTGVSAAMDAPI 145
           S+LL+  N+ L  ++ +CL +CLELYS+A S L   +  L   DY  AN  VSAAMDA +
Sbjct: 86  SKLLQ--NKTLDHFARDCLTTCLELYSDAYSTLRGVVDDLGSRDYYKANVDVSAAMDAAV 145

Query: 146 SCEDGYREKDGEVSPFTEINDGFFQLIAISLAFINM 182
           +CEDG++E++G VSP T+ ND FFQL AISLAFI M
Sbjct: 146 TCEDGFKEREGLVSPLTKENDMFFQLNAISLAFIKM 178

[Close]

BLAST of Lsi05G000450 vs. NCBI nr
Match: gi|590675981|ref|XP_007039604.1| (Plant invertase/pectin methylesterase inhibitor superfamily protein, putative [Theobroma cacao])

HSP 1 Score: 140.2 bits (352), Expect = 3.6e-30
Identity = 79/181 (43.65%), Postives = 115/181 (63.54%), Query Frame = 1


Query: 1   MENPISKSISLLLFIPTFLQLAYCLDIVQQSCKLAAQTDPYVNYKLCVQSLKANPNYSKA 60
           MEN      + + F    L ++   D++Q SC  AA+ D  +N+  CV SL+ NP    A
Sbjct: 74  MENSFPSVCTFIAFF-FLLLVSASSDVIQDSCDKAAKGDRNINFDFCVASLEGNPKSKTA 133

Query: 61  ANFKDLLMISINHSKANATEIGSQISELLKGSNEKLGKYSLNCLKSCLELYSEAISDLEK 120
            + + L+ ISI  + +NAT I S IS+LLK  N+ L   + +CL+ C ELYS+A  +LE 
Sbjct: 134 TSTESLVPISIEMAISNATSISSIISKLLK--NKNLDNPTRSCLEDCSELYSDAQPNLEI 193

Query: 121 ALGALKMEDYETANTGVSAAMDAPISCEDGYREKDGEVSPFTEINDGFFQLIAISLAFIN 180
              A++ +DY TA T +SAA+DAP++CEDG++E++G VSP T+ ND FFQL  + LAFIN
Sbjct: 194 GGQAVESKDYGTAKTVISAALDAPVTCEDGFKEREGLVSPLTKENDEFFQLTVVPLAFIN 251

Query: 181 M 182
           +
Sbjct: 254 L 251

The following BLAST results are available for this feature:

BLAST of Lsi05G000450 vs. Swiss-Prot


Match Name	E-value	Identity	Description
PLA1_PLAAC	1.5e-23	40.11	Putative invertase inhibitor OS=Platanus acerifolia PE=1 SV=1	[more]

BLAST of Lsi05G000450 vs. TrEMBL


Match Name	E-value	Identity	Description
A0A0A0LHE9_CUCSA	1.4e-73	80.11	Uncharacterized protein OS=Cucumis sativus GN=Csa_3G910710 PE=4 SV=1	[more]
A0A059C0T9_EUCGR	1.1e-30	50.64	Uncharacterized protein OS=Eucalyptus grandis GN=EUGRSUZ_E00321 PE=4 SV=1	[more]
A0A061G2M1_THECC	2.5e-30	43.65	Plant invertase/pectin methylesterase inhibitor superfamily protein, putative OS...	[more]
M5WAV9_PRUPE	4.3e-30	49.68	Uncharacterized protein OS=Prunus persica GN=PRUPE_ppa012101mg PE=4 SV=1	[more]
A0A0D2PIZ3_GOSRA	3.6e-29	48.00	Uncharacterized protein OS=Gossypium raimondii GN=B456_001G141900 PE=4 SV=1	[more]

BLAST of Lsi05G000450 vs. TAIR10


Match Name	E-value	Identity	Description
AT5G46940.1	4.0e-27	37.99	Plant invertase/pectin methylesterase inhibitor superfamily protein	[more]
AT5G46930.1	5.9e-23	34.86	Plant invertase/pectin methylesterase inhibitor superfamily protein	[more]
AT5G46970.1	1.0e-22	40.28	Plant invertase/pectin methylesterase inhibitor superfamily protein	[more]
AT5G46960.1	8.0e-20	34.68	Plant invertase/pectin methylesterase inhibitor superfamily protein	[more]
AT5G46950.1	3.0e-19	34.10	Plant invertase/pectin methylesterase inhibitor superfamily protein	[more]

BLAST of Lsi05G000450 vs. NCBI nr


Match Name	E-value	Identity	Description
gi\|449461581\|ref\|XP_004148520.1\|	2.0e-73	80.11	PREDICTED: putative invertase inhibitor [Cucumis sativus]	[more]
gi\|659131910\|ref\|XP_008465919.1\|	1.2e-70	77.90	PREDICTED: putative invertase inhibitor [Cucumis melo]	[more]
gi\|645246780\|ref\|XP_008229513.1\|	5.0e-32	50.97	PREDICTED: putative invertase inhibitor [Prunus mume]	[more]
gi\|702353236\|ref\|XP_010058175.1\|	1.6e-30	50.64	PREDICTED: putative invertase inhibitor [Eucalyptus grandis]	[more]
gi\|590675981\|ref\|XP_007039604.1\|	3.6e-30	43.65	Plant invertase/pectin methylesterase inhibitor superfamily protein, putative [T...	[more]

Annotated Terms

The following terms have been associated with this gene:

Vocabulary: **Molecular Function**
Term	Definition
GO:0004857	enzyme inhibitor activity

Vocabulary: **INTERPRO**
Term	Definition
IPR006501	Pectinesterase_inhib_dom

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0043086	negative regulation of catalytic activity
biological_process	GO:0008152	metabolic process
biological_process	GO:0005982	starch metabolic process
biological_process	GO:0007264	small GTPase mediated signal transduction
biological_process	GO:0006913	nucleocytoplasmic transport
biological_process	GO:0008150	biological_process
biological_process	GO:0006886	intracellular protein transport
biological_process	GO:0005985	sucrose metabolic process
cellular_component	GO:0005575	cellular_component
cellular_component	GO:0005768	endosome
cellular_component	GO:0000139	Golgi membrane
cellular_component	GO:0005886	plasma membrane
cellular_component	GO:0009506	plasmodesma
cellular_component	GO:0005802	trans-Golgi network
cellular_component	GO:0005774	vacuolar membrane
cellular_component	GO:0005829	cytosol
molecular_function	GO:0030599	pectinesterase activity
molecular_function	GO:0004857	enzyme inhibitor activity
molecular_function	GO:0003924	GTPase activity
molecular_function	GO:0005525	GTP binding

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Lsi05G000450.1	Lsi05G000450.1	mRNA

InterPro

Analysis Name: InterPro Annotations of Lagenaria siceraria
Date Performed: 2017-09-18


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR006501	Pectinesterase inhibitor domain	GENE3D	G3DSA:1.20.140.40		coord: 26..181 score: 2.3
IPR006501	Pectinesterase inhibitor domain	PFAM	PF04043	PMEI	coord: 27..177 score: 8.5
IPR006501	Pectinesterase inhibitor domain	SMART	SM00856	PMEI_2	coord: 23..178 score: 9.9
IPR006501	Pectinesterase inhibitor domain	TIGRFAMs	TIGR01614	TIGR01614	coord: 1..181 score: 1.6
IPR006501	Pectinesterase inhibitor domain	unknown	SSF101148	Plant invertase/pectin methylesterase inhibitor	coord: 26..181 score: 3.01
None	No IPR available	unknown	Coil	Coil	coord: 108..128 scor
None	No IPR available	PANTHER	PTHR31707	FAMILY NOT NAMED	coord: 1..181 score: 9.5
None	No IPR available	PANTHER	PTHR31707:SF47	PLANT INVERTASE/PECTIN METHYLESTERASE INHIBITOR DOMAIN-CONTAINING PROTEIN	coord: 1..181 score: 9.5

Synteny

The following gene(s) are orthologous to this gene:


Gene	Orthologue	Organism	Block
Lsi05G000450	Csa3G910710	Cucumber (Chinese Long) v2	culsiB213
Lsi05G000450	Csa3G128870	Cucumber (Chinese Long) v2	culsiB223
Lsi05G000450	CSPI03G10270	Wild cucumber (PI 183967)	cpilsiB219
Lsi05G000450	CSPI03G47160	Wild cucumber (PI 183967)	cpilsiB211
Lsi05G000450	Cucsa.313560	Cucumber (Gy14) v1	cgylsiB506
Lsi05G000450	Cucsa.256940	Cucumber (Gy14) v1	cgylsiB419
Lsi05G000450	CmaCh19G003750	Cucurbita maxima (Rimu)	cmalsiB490
Lsi05G000450	CmaCh02G015850	Cucurbita maxima (Rimu)	cmalsiB579
Lsi05G000450	CmoCh02G016270	Cucurbita moschata (Rifu)	cmolsiB556
Lsi05G000450	CmoCh19G003790	Cucurbita moschata (Rifu)	cmolsiB473
Lsi05G000450	Cla021374	Watermelon (97103) v1	lsiwmB370
Lsi05G000450	Cla016135	Watermelon (97103) v1	lsiwmB347
Lsi05G000450	MELO3C003367	Melon (DHL92) v3.5.1	lsimeB351
Lsi05G000450	MELO3C006266	Melon (DHL92) v3.5.1	lsimeB386
Lsi05G000450	MELO3C006266.2	Melon (DHL92) v3.6.1	lsimedB407
Lsi05G000450	MELO3C003367.2	Melon (DHL92) v3.6.1	lsimedB366
Lsi05G000450	Cla97C05G083460	Watermelon (97103) v2	lsiwmbB337
Lsi05G000450	Cla97C09G175280	Watermelon (97103) v2	lsiwmbB353
Lsi05G000450	Bhi05G000354	Wax gourd	lsiwgoB457
Lsi05G000450	Bhi01G000113	Wax gourd	lsiwgoB455
Lsi05G000450	Cp4.1LG09g06690	Cucurbita pepo (Zucchini)	cpelsiB038
Lsi05G000450	Cp4.1LG05g01970	Cucurbita pepo (Zucchini)	cpelsiB621
Lsi05G000450	CsGy3G010390	Cucumber (Gy14) v2	cgyblsiB211
Lsi05G000450	CsGy3G044550	Cucumber (Gy14) v2	cgyblsiB200
Lsi05G000450	Carg08815	Silver-seed gourd	carlsiB716
Lsi05G000450	CsaV3_3G010420	Cucumber (Chinese Long) v3	cuclsiB242
Lsi05G000450	CsaV3_3G049590	Cucumber (Chinese Long) v3	cuclsiB232

The following gene(s) are paralogous to this gene:


Gene	Paralogue	Organism	Block
Lsi05G000450	Lsi05G018940	Bottle gourd (USVL1VR-Ls)	lsilsiB159

The following block(s) are covering this gene:


Gene	Organism	Block
Lsi05G000450	Bottle gourd (USVL1VR-Ls)	lsilsiB162
Lsi05G000450	Cucumber (Gy14) v1	cgylsiB529
Lsi05G000450	Cucurbita maxima (Rimu)	cmalsiB319
Lsi05G000450	Cucurbita maxima (Rimu)	cmalsiB488
Lsi05G000450	Cucurbita maxima (Rimu)	cmalsiB763
Lsi05G000450	Cucurbita moschata (Rifu)	cmolsiB309
Lsi05G000450	Cucurbita moschata (Rifu)	cmolsiB472
Lsi05G000450	Cucurbita moschata (Rifu)	cmolsiB475
Lsi05G000450	Cucurbita moschata (Rifu)	cmolsiB755
Lsi05G000450	Cucurbita pepo (Zucchini)	cpelsiB180
Lsi05G000450	Cucurbita pepo (Zucchini)	cpelsiB210
Lsi05G000450	Wild cucumber (PI 183967)	cpilsiB218
Lsi05G000450	Cucumber (Chinese Long) v2	culsiB219
Lsi05G000450	Melon (DHL92) v3.5.1	lsimeB372
Lsi05G000450	Watermelon (Charleston Gray)	lsiwcgB315
Lsi05G000450	Watermelon (Charleston Gray)	lsiwcgB320
Lsi05G000450	Watermelon (Charleston Gray)	lsiwcgB341
Lsi05G000450	Watermelon (97103) v1	lsiwmB350
Lsi05G000450	Cucumber (Gy14) v2	cgyblsiB205
Lsi05G000450	Melon (DHL92) v3.6.1	lsimedB403
Lsi05G000450	Silver-seed gourd	carlsiB512
Lsi05G000450	Silver-seed gourd	carlsiB643
Lsi05G000450	Silver-seed gourd	carlsiB941
Lsi05G000450	Cucumber (Chinese Long) v3	cuclsiB237
Lsi05G000450	Watermelon (97103) v2	lsiwmbB324
Lsi05G000450	Wax gourd	lsiwgoB411
Lsi05G000450	Wax gourd	lsiwgoB460