Lsi04G006220 (gene) Bottle gourd (USVL1VR-Ls) | Cucurbit Genomics Database (CuGenDB)

Overview


Name	Lsi04G006220
Type	gene
Organism	Lagenaria siceraria (Bottle gourd (USVL1VR-Ls))
Description	Cytochrome P450
Location	chr04 : 5677055 .. 5678955 (-)

Genome Viewer

Gene Expression

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: CDS

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGCTCCTCAAACTCGGCTCAGTCCCGACCCTCGTAATCTCCTCCTCCGCCGCCGCAAAAGAGATTCTAAAAGTTAACGACCTCTCTTGTTGCAGCCGCCCTCGTTTAACCGCGGGTGGAAGATTTTCATACAAGTATTTAGACATTGTGTTAGGACAATATGGTGATCATTGGAGAGAAGTTCGAAAGATTTGTGTGGTTGGGTTGTTTAATGCTAGACGAGTGCAATCATTTCAAATGATAAGACAAGAAGAGATTGATGTGTTGTTAAATTCCATCTCTCAATCTTCATCTTCTGCCACTTCAATTAATTTAAGTGAGAAATTTTATTCTTTCACTATAAATATGACAACACGGATTGCGTTTGGGAAGAAGTTTAGAGGGGGTGAGATAGATAATGATAATATTGAAAATGTTATACGTAGAGCAGTAGTAGTATTGAAAAGCTTTTCAGCTACTGATTTCTTTCCTAGAGTGGGTTGGATTATTGATTGGCTTAGTGGTTTTCATACAAGGATGGAGAAGAGTTTTACTGAATTGGATGGTTATTTTCAACATGTTTTGGATTATCACATCAAATTTAGGAAATTTTCTGGGTGTGATGATGAAGAAAATATTGTTGATGTTTTGTTGAAAAAAGCAAGGGAGGATGTCCCTAAATTTGATGCAGATTGCATTAAGGCACTTCTCATGGTAAAGTTTCAACTCAATATAACACATCTCCCTTTCAAACCCCTTCACAAGGTTTTCACAAGTGGAAGACCAATCATTTATATTTGGATAATTTTTAAAAATAGAAAAATAAGGAAAATTATTTACACAAAATAGCAAAATTTTTAGATAGTTGTGTGATAGATGCTGAAAAAAGTTTATCAAGGTCTATCAGTGATAGAAATGATAGAAGTCTATCACTGATAGAAGTTTATCAATGTTTATCAGTGGTTTTTTTTGCTATTTTCTGTAAATACTTTGATATTTTTTTCTACCTGTGAAAATTTTCCTTTATATTTTTCCATATATCTTTTAAACTTTATGAGTTTTGATACCATATTTAAATAAATATGAAATTTTTATTTCAAGATTAATTAAATATGAAAGAGTAACTCGTGTCATATATATAATAATGCAAGTTTTGAATTCCTCTAGTTTTCTAATTTAGGATCTCGAAGATATTAAGATTTTTCTTGTATTCTCAATAAGTTATAGATTGCTAACCTTTGTGAGTTTTTTTCTTTCTTCTTATTTTCTTTTGAGTTCTTAATTTTTGGAAGGGTGTGTGTTTCCAGGATATACTATTAGCTGGAGTAGAAACAGGAGCAAACACCCTTGTTTGGGCGTTGACAGAATTGGTAAAGAATCCAAAGGTGATGAAGAAGCTGCAAGATGAAATTAGAAGTTGCATAAAAGAAGATCGGGTGAAAGAGAGCGATCTCGAAAAGCTTAAATATCTAAAAGTAGTGATGAAAGAAGTTCTAAGGTTGCATGCACTTGCTCCACTTCTGCTTCCAAGAGAAACAATGTCCCATTTTAAGTTGAATGGTTATGATATTGATCCAAAAACTCTTGTTTATATAAATGTGTGGGCAATCGGACGAGACCCAAAATTATGGACAAATCCAGAAGAGTTCTTGCCGGAGAGATTTATAGGAAGTGGTATTGATTACAAAAGACATAATTTTGAGTTATTGTCATTTGGATCTGGTCGGAGAATTTGTCCGGAGATGAATATGGCAACCATTATGATGGAGCTAACATTGGCTAATATGTTATTGTGTTTTGATTGGAAATTGCCTGCAAATATGAAAAGCAAAGATGTGGGCGTTGAAGAGGATTCTAGTTTTGTAGTTGCCAAAAAATCGCCTCTTCAACTTGTTCCAATATGTTATTTCAACTCTATTTAA

mRNA sequence

ATGCTCCTCAAACTCGGCTCAGTCCCGACCCTCGTAATCTCCTCCTCCGCCGCCGCAAAAGAGATTCTAAAAGTTAACGACCTCTCTTGTTGCAGCCGCCCTCGTTTAACCGCGGGTGGAAGATTTTCATACAAGTATTTAGACATTGTGTTAGGACAATATGGTGATCATTGGAGAGAAGTTCGAAAGATTTGTGTGGTTGGGTTGTTTAATGCTAGACGAGTGCAATCATTTCAAATGATAAGACAAGAAGAGATTGATGTGTTGTTAAATTCCATCTCTCAATCTTCATCTTCTGCCACTTCAATTAATTTAAGTGAGAAATTTTATTCTTTCACTATAAATATGACAACACGGATTGCGTTTGGGAAGAAGTTTAGAGGGGGTGAGATAGATAATGATAATATTGAAAATGTTATACGTAGAGCAGTAGTAGTATTGAAAAGCTTTTCAGCTACTGATTTCTTTCCTAGAGTGGGTTGGATTATTGATTGGCTTAGTGGTTTTCATACAAGGATGGAGAAGAGTTTTACTGAATTGGATGGTTATTTTCAACATGTTTTGGATTATCACATCAAATTTAGGAAATTTTCTGGGTGTGATGATGAAGAAAATATTGTTGATGTTTTGTTGAAAAAAGCAAGGGAGGATGTCCCTAAATTTGATGCAGATTGCATTAAGGCACTTCTCATGGATATACTATTAGCTGGAGTAGAAACAGGAGCAAACACCCTTGTTTGGGCGTTGACAGAATTGGTAAAGAATCCAAAGGTGATGAAGAAGCTGCAAGATGAAATTAGAAGTTGCATAAAAGAAGATCGGGTGAAAGAGAGCGATCTCGAAAAGCTTAAATATCTAAAAGTAGTGATGAAAGAAGTTCTAAGGTTGCATGCACTTGCTCCACTTCTGCTTCCAAGAGAAACAATGTCCCATTTTAAGTTGAATGGTTATGATATTGATCCAAAAACTCTTGTTTATATAAATGTGTGGGCAATCGGACGAGACCCAAAATTATGGACAAATCCAGAAGAGTTCTTGCCGGAGAGATTTATAGGAAGTGGTATTGATTACAAAAGACATAATTTTGAGTTATTGTCATTTGGATCTGGTCGGAGAATTTGTCCGGAGATGAATATGGCAACCATTATGATGGAGCTAACATTGGCTAATATGTTATTGTGTTTTGATTGGAAATTGCCTGCAAATATGAAAAGCAAAGATGTGGGCGTTGAAGAGGATTCTAGTTTTGTAGTTGCCAAAAAATCGCCTCTTCAACTTGTTCCAATATGTTATTTCAACTCTATTTAA

Coding sequence (CDS)

ATGCTCCTCAAACTCGGCTCAGTCCCGACCCTCGTAATCTCCTCCTCCGCCGCCGCAAAAGAGATTCTAAAAGTTAACGACCTCTCTTGTTGCAGCCGCCCTCGTTTAACCGCGGGTGGAAGATTTTCATACAAGTATTTAGACATTGTGTTAGGACAATATGGTGATCATTGGAGAGAAGTTCGAAAGATTTGTGTGGTTGGGTTGTTTAATGCTAGACGAGTGCAATCATTTCAAATGATAAGACAAGAAGAGATTGATGTGTTGTTAAATTCCATCTCTCAATCTTCATCTTCTGCCACTTCAATTAATTTAAGTGAGAAATTTTATTCTTTCACTATAAATATGACAACACGGATTGCGTTTGGGAAGAAGTTTAGAGGGGGTGAGATAGATAATGATAATATTGAAAATGTTATACGTAGAGCAGTAGTAGTATTGAAAAGCTTTTCAGCTACTGATTTCTTTCCTAGAGTGGGTTGGATTATTGATTGGCTTAGTGGTTTTCATACAAGGATGGAGAAGAGTTTTACTGAATTGGATGGTTATTTTCAACATGTTTTGGATTATCACATCAAATTTAGGAAATTTTCTGGGTGTGATGATGAAGAAAATATTGTTGATGTTTTGTTGAAAAAAGCAAGGGAGGATGTCCCTAAATTTGATGCAGATTGCATTAAGGCACTTCTCATGGATATACTATTAGCTGGAGTAGAAACAGGAGCAAACACCCTTGTTTGGGCGTTGACAGAATTGGTAAAGAATCCAAAGGTGATGAAGAAGCTGCAAGATGAAATTAGAAGTTGCATAAAAGAAGATCGGGTGAAAGAGAGCGATCTCGAAAAGCTTAAATATCTAAAAGTAGTGATGAAAGAAGTTCTAAGGTTGCATGCACTTGCTCCACTTCTGCTTCCAAGAGAAACAATGTCCCATTTTAAGTTGAATGGTTATGATATTGATCCAAAAACTCTTGTTTATATAAATGTGTGGGCAATCGGACGAGACCCAAAATTATGGACAAATCCAGAAGAGTTCTTGCCGGAGAGATTTATAGGAAGTGGTATTGATTACAAAAGACATAATTTTGAGTTATTGTCATTTGGATCTGGTCGGAGAATTTGTCCGGAGATGAATATGGCAACCATTATGATGGAGCTAACATTGGCTAATATGTTATTGTGTTTTGATTGGAAATTGCCTGCAAATATGAAAAGCAAAGATGTGGGCGTTGAAGAGGATTCTAGTTTTGTAGTTGCCAAAAAATCGCCTCTTCAACTTGTTCCAATATGTTATTTCAACTCTATTTAA

Protein sequence

MLLKLGSVPTLVISSSAAAKEILKVNDLSCCSRPRLTAGGRFSYKYLDIVLGQYGDHWREVRKICVVGLFNARRVQSFQMIRQEEIDVLLNSISQSSSSATSINLSEKFYSFTINMTTRIAFGKKFRGGEIDNDNIENVIRRAVVVLKSFSATDFFPRVGWIIDWLSGFHTRMEKSFTELDGYFQHVLDYHIKFRKFSGCDDEENIVDVLLKKAREDVPKFDADCIKALLMDILLAGVETGANTLVWALTELVKNPKVMKKLQDEIRSCIKEDRVKESDLEKLKYLKVVMKEVLRLHALAPLLLPRETMSHFKLNGYDIDPKTLVYINVWAIGRDPKLWTNPEEFLPERFIGSGIDYKRHNFELLSFGSGRRICPEMNMATIMMELTLANMLLCFDWKLPANMKSKDVGVEEDSSFVVAKKSPLQLVPICYFNSI

Homology

[Close]

BLAST of Lsi04G006220 vs. Swiss-Prot
Match: C71BX_ARATH (Cytochrome P450 71B36 OS=Arabidopsis thaliana GN=CYP71B36 PE=3 SV=1)

HSP 1 Score: 431.8 bits (1109), Expect = 8.9e-120
Identity = 212/438 (48.40%), Postives = 301/438 (68.72%), Query Frame = 1


Query: 1   MLLKLGSVPTLVISSSAAAKEILKVNDLSCCSRPRLTAGGRFSYKYLDIVLGQYGDHWRE 60
           MLLK GS+PT+V+SSS  AK++LK++DL CCSRP L      SY YLDI    + D+W+E
Sbjct: 66  MLLKFGSIPTVVVSSSETAKQVLKIHDLHCCSRPSLAGPRALSYNYLDIAFSPFDDYWKE 125

Query: 61  VRKICVVGLFNARRVQSFQMIRQEEIDVLLNSISQSSSSATSINLSEKFYSFTINMTTRI 120
           +R+ICV  LF+ +RVQSFQ I+++E+  L++S+S+S+S  T +NLSEKF S T+ +T + 
Sbjct: 126 LRRICVQELFSVKRVQSFQPIKEDEVKKLIDSVSESASQGTPVNLSEKFTSLTVRVTCKA 185

Query: 121 AFGKKFRGGEIDNDNIENVIRRAVVVLKSFSATDFFPRVGWIIDWLSGFHTRMEKSFTEL 180
            FG  F+G  +++D  E +I    + L SFSA+D+FP  GWIIDWL+G H + E+S   L
Sbjct: 186 TFGVNFQGTVLNSDRFEKLIHDTYLFLGSFSASDYFPNGGWIIDWLTGLHGQRERSVRAL 245

Query: 181 DGYFQHVLDYHIKFRKFSGCDDEENIVDVLLKKAREDV----PKFDADCIKALLMDILLA 240
           D +++ + D H +  K    +  E+ VD+LL+  +E+      K   + IKA+LM++L+ 
Sbjct: 246 DAFYEQMFDLHKQGNK----EGVEDFVDLLLRLEKEETVIGYGKLTRNHIKAILMNVLIG 305

Query: 241 GVETGANTLVWALTELVKNPKVMKKLQDEIRSCI-KEDRVKESDLEKLKYLKVVMKEVLR 300
           G+ T A T+ WA+TEL++NP+VMKK+Q EIR+ I K+  +   D+++L YLK+V+ E  R
Sbjct: 306 GIGTSAITMTWAMTELMRNPRVMKKVQSEIRNQIGKKSMITLDDIDQLHYLKMVINETWR 365

Query: 301 LHALAPLLLPRETMSHFKLNGYDIDPKTLVYINVWAIGRDPKLWTNPEEFLPERFIGSGI 360
           LH  +P L+PR+ MS F+LN Y I  KT +Y+NVWAIGRDP  W +PEEFLPERF+ S I
Sbjct: 366 LHPPSPFLIPRQVMSEFELNDYVIPVKTRLYVNVWAIGRDPDTWKDPEEFLPERFVNSSI 425

Query: 361 DYKRHNFELLSFGSGRRICPEMNMATIMMELTLANMLLCFDWKLPANMKSKDVGVEEDSS 420
           D K  +FELL FGSGRR+CP M M T M+E  LANML  FDWK+P  M ++D+ +EE   
Sbjct: 426 DAKGQHFELLPFGSGRRMCPAMYMGTTMVEFGLANMLYHFDWKIPVGMVAEDIDLEESPG 485

Query: 421 FVVAKKSPLQLVPICYFN 434
              +KK+ L LVP+ Y +
Sbjct: 486 LNASKKNELVLVPLKYLD 499

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BLAST of Lsi04G006220 vs. Swiss-Prot
Match: C71BY_ARATH (Cytochrome P450 71B37 OS=Arabidopsis thaliana GN=CYP71B37 PE=3 SV=2)

HSP 1 Score: 426.8 bits (1096), Expect = 2.9e-118
Identity = 215/438 (49.09%), Postives = 297/438 (67.81%), Query Frame = 1


Query: 1   MLLKLGSVPTLVISSSAAAKEILKVNDLSCCSRPRLTAGGRFSYKYLDIVLGQYGDHWRE 60
           MLLK GS+PT+V+SSS  AK+ LK++DL+CCSRP L      SY YLDIV   + D+W+E
Sbjct: 66  MLLKFGSIPTVVVSSSETAKQALKIHDLNCCSRPSLAGPRALSYNYLDIVFSPFNDYWKE 125

Query: 61  VRKICVVGLFNARRVQSFQMIRQEEIDVLLNSISQSSSSATSINLSEKFYSFTINMTTRI 120
           +R++CV  LF+ ++V   Q IR+EE+  L+NS S+S++  T +NLSEK  S T+ +  + 
Sbjct: 126 LRRMCVQELFSPKQVHLIQPIREEEVKKLMNSFSESAAQKTPVNLSEKLASLTVGVICKA 185

Query: 121 AFGKKFRGGEIDNDNIENVIRRAVVVLKSFSATDFFPRVGWIIDWLSGFHTRMEKSFTEL 180
           AFG  F+G  +++DN + +I  A + L SFSA+D+FP VGWIIDWL+G   + E+S   L
Sbjct: 186 AFGVSFQGTVLNSDNFDKLIHDAFLFLGSFSASDYFPNVGWIIDWLTGLQGQRERSVRGL 245

Query: 181 DGYFQHVLDYHIKFRKFSGCDDEENIVDVLLKKAREDV----PKFDADCIKALLMDILLA 240
           D +++ + D H +  K    +  E+ VD+LLK  +E+      K   + IKA+LM++LL 
Sbjct: 246 DAFYEQMFDLHKQGNK----EGVEDFVDLLLKLEKEETVLGYGKLTRNHIKAVLMNVLLG 305

Query: 241 GVETGANTLVWALTELVKNPKVMKKLQDEIRSCI-KEDRVKESDLEKLKYLKVVMKEVLR 300
           G+ T A T+ WA+TEL++NP+VMKK+Q EIR+ I  +  +   D+++L YLK+V+ E  R
Sbjct: 306 GIGTSAITMTWAMTELMRNPRVMKKVQSEIRNQIGGKSMICLDDIDQLHYLKMVINETWR 365

Query: 301 LHALAPLLLPRETMSHFKLNGYDIDPKTLVYINVWAIGRDPKLWTNPEEFLPERFIGSGI 360
           LH  APLL+PRE MS F++NGY I  KT +Y+NVW IGRDP  W +PEEFLPERF+ S I
Sbjct: 366 LHPPAPLLVPREVMSEFEINGYTIPAKTRLYVNVWGIGRDPDTWKDPEEFLPERFVNSNI 425

Query: 361 DYKRHNFELLSFGSGRRICPEMNMATIMMELTLANMLLCFDWKLPANMKSKDVGVEEDSS 420
           D K  NFELL FGSGRR+CP M M T M+E  LAN+L  FDWKLP  M  +D+ +EE   
Sbjct: 426 DAKGQNFELLPFGSGRRMCPAMYMGTTMVEFGLANLLYHFDWKLPEGMVVEDIDMEESPG 485

Query: 421 FVVAKKSPLQLVPICYFN 434
              +KK+ L LVP  Y N
Sbjct: 486 LNASKKNELVLVPRKYLN 499

[Close]

BLAST of Lsi04G006220 vs. Swiss-Prot
Match: C71B2_ARATH (Cytochrome P450 71B2 OS=Arabidopsis thaliana GN=CYP71B2 PE=2 SV=2)

HSP 1 Score: 424.9 bits (1091), Expect = 1.1e-117
Identity = 203/434 (46.77%), Postives = 297/434 (68.43%), Query Frame = 1


Query: 1   MLLKLGSVPTLVISSSAAAKEILKVNDLSCCSRPRLTAGGRFSYKYLDIVLGQYGDHWRE 60
           + L+LGSVP +VISSS AA+ +LK NDL CCSRP+    G+ SY + DI    YG++WRE
Sbjct: 66  VFLRLGSVPVVVISSSEAAEAVLKTNDLECCSRPKTVGSGKLSYGFKDITFAPYGEYWRE 125

Query: 61  VRKICVVGLFNARRVQSFQMIRQEEIDVLLNSISQSSSSATSINLSEKFYSFTINMTTRI 120
           VRK+ V+ LF++++VQSF+ IR+EE+D ++  +S+S+   + ++LS+ F+S T ++  R+
Sbjct: 126 VRKLAVIELFSSKKVQSFRYIREEEVDFVVKKVSESALKQSPVDLSKTFFSLTASIICRV 185

Query: 121 AFGKKFR--GGEIDNDNIENVIRRAVVVLKSFSATDFFPR-VGWIIDWLSGFHTRMEKSF 180
           A G+ F   G  ID D IE ++  +   L +F+ +DFFP  +G  +DWL   H ++ K F
Sbjct: 186 ALGQNFNESGFVIDQDRIEELVTESAEALGTFTFSDFFPGGLGRFVDWLFQRHKKINKVF 245

Query: 181 TELDGYFQHVLDYHIKFRKFSGCDDEENIVDVLLKKAREDVPKFDADCIKALLMDILLAG 240
            ELD ++QHV+D H+K       D    I+D++ K+   D  K + D +KA++MD+ LAG
Sbjct: 246 KELDAFYQHVIDDHLKPEGRKNQDIVTLILDMIDKQEDSDSFKLNMDNLKAIVMDVFLAG 305

Query: 241 VETGANTLVWALTELVKNPKVMKKLQDEIRSCI--KEDRVKESDLEKLKYLKVVMKEVLR 300
           ++T A T++WA+TEL++NP+VMKK Q+ IR+ +  K++R+ E DL K++YL  ++KE  R
Sbjct: 306 IDTSAVTMIWAMTELIRNPRVMKKAQESIRTTLGLKKERITEEDLGKVEYLNHILKETFR 365

Query: 301 LHALAPLLLPRETMSHFKLNGYDIDPKTLVYINVWAIGRDPKLWTNPEEFLPERFIGSGI 360
           LH   P ++PRETMSH K+ GYDI PKT + +NVW IGRDPK W +PEEF PERF  S +
Sbjct: 366 LHPALPFVVPRETMSHIKIQGYDIPPKTQIQLNVWTIGRDPKRWNDPEEFNPERFANSSV 425

Query: 361 DYKRHNFELLSFGSGRRICPEMNMATIMMELTLANMLLCFDWKLPANMKSKDVGVEEDSS 420
           D++  +F+LL FGSGRRICP M MA   +EL L N+L  FDW +P   K +D+ +EE  +
Sbjct: 426 DFRGQHFDLLPFGSGRRICPGMPMAIASVELALMNLLYYFDWSMPDGTKGEDIDMEEAGN 485

Query: 421 FVVAKKSPLQLVPI 430
             + KK PLQLVP+
Sbjct: 486 ISIVKKIPLQLVPV 499

[Close]

BLAST of Lsi04G006220 vs. Swiss-Prot
Match: C71B9_ARATH (Cytochrome P450 71B9 OS=Arabidopsis thaliana GN=CYP71B9 PE=2 SV=3)

HSP 1 Score: 423.7 bits (1088), Expect = 2.4e-117
Identity = 214/436 (49.08%), Postives = 291/436 (66.74%), Query Frame = 1


Query: 1   MLLKLGSVPTLVISSSAAAKEILKVNDLSCCSRPRLTAGGRFSYKYLDIVLGQYGDHWRE 60
           MLLKLGSVPT+V+SSS  AK++LK+NDL CCSRP L      SY YLDI    + D+W+E
Sbjct: 66  MLLKLGSVPTVVVSSSETAKQVLKINDLHCCSRPSLAGAKELSYNYLDIAFSPFDDYWKE 125

Query: 61  VRKICVVGLFNARRVQSFQMIRQEEIDVLLNSISQSSSSATSINLSEKFYSFTINMTTRI 120
           +R+ICV  LF+A+RV S Q I++EE+  L+ S ++S+S  + +NLSEKF   T+++  + 
Sbjct: 126 LRRICVQELFSAKRVHSIQPIKEEEVRKLIVSATESASQKSPVNLSEKFLDLTVSVICKA 185

Query: 121 AFGKKFRGGEIDNDNIENVIRRAVVVLKSFSATDFFPRVGWIIDWLSGFHTRMEKSFTEL 180
           AF   F    ++ND  + +I  A + L SFSA++FFP  GWIIDWL+G   R EKS  +L
Sbjct: 186 AFSLDFHTSVLNNDGFDKLIHDAFLFLGSFSASNFFPNGGWIIDWLTGLQRRREKSVKDL 245

Query: 181 DGYFQHVLDYHIKFRKFSGCDDEENIVDVLLKKAREDV----PKFDADCIKALLMDILLA 240
           D ++Q + D H +  K       E+ VD+LLK  +E+      K   + +KA+LM++LL 
Sbjct: 246 DVFYQQMFDLHKQENK----QGVEDFVDLLLKLEKEETVLGYGKLTRNHVKAILMNVLLG 305

Query: 241 GVETGANTLVWALTELVKNPKVMKKLQDEIRS-CIKEDRVKESDLEKLKYLKVVMKEVLR 300
            + T A T+ WA+ EL++NP+VMKK+Q EIR+  I +  +   D++ L YLK+V+KE  R
Sbjct: 306 AINTSAMTMTWAMAELIRNPRVMKKVQSEIRNQMINKSVITLDDIDHLPYLKMVIKETWR 365

Query: 301 LHALAPLLLPRETMSHFKLNGYDIDPKTLVYINVWAIGRDPKLWTNPEEFLPERFIGSGI 360
           LH   PLLLPRE MS F++NGY I PKTL+Y+NVWAIGRDP  W + + F PERF+ + I
Sbjct: 366 LHPPVPLLLPREVMSEFEINGYKIQPKTLLYVNVWAIGRDPDSWKDADMFYPERFMDNNI 425

Query: 361 DYKRHNFELLSFGSGRRICPEMNMATIMMELTLANMLLCFDWKLPANMKSKDVGVEEDSS 420
           D K  NFELL FGSGRRICP M M T M+E  LANML  FDW++P  M  +D+ +EE   
Sbjct: 426 DAKGQNFELLPFGSGRRICPGMYMGTTMVEFGLANMLYQFDWEVPDGMVVEDIDMEESPG 485

Query: 421 FVVAKKSPLQLVPICY 432
             V KK+ L LVP+ Y
Sbjct: 486 LAVGKKNELLLVPVKY 497

[Close]

BLAST of Lsi04G006220 vs. Swiss-Prot
Match: C71BQ_ARATH (Cytochrome P450 71B26 OS=Arabidopsis thaliana GN=CYP71B26 PE=2 SV=1)

HSP 1 Score: 414.5 bits (1064), Expect = 1.5e-114
Identity = 214/438 (48.86%), Postives = 294/438 (67.12%), Query Frame = 1


Query: 1   MLLKLGSVPTLVISSSAAAKEILKVNDLSCCSRPRLTAGGRFSYKYLDIVLGQYGDHWRE 60
           MLLKLG VPTL++SSS  AK+ L+  DL CCSRP L  G   SY  LD+    Y ++W+E
Sbjct: 66  MLLKLGKVPTLILSSSETAKQALRDYDLHCCSRPSLAGGRELSYNNLDMSSSPYNEYWKE 125

Query: 61  VRKICVVGLFNARRVQSFQMIRQEEIDVLLNSISQSSSSATSINLSEKFYSFTINMTTRI 120
           +RK+C   LF+A ++QS Q I+ EE+  +++SI++SSS    +NLS+ F + T ++  + 
Sbjct: 126 LRKLCSQELFSANKIQSIQPIKDEEVKKVIDSIAESSSLKNPVNLSKTFLALTTSVVCKA 185

Query: 121 AFGKKFRGGEIDNDNIENVIRRAVVVLKSFSATDFFPRVGWIIDWLSGFHTRMEKSFTEL 180
           AFG  F G  +++D    ++R    +L SFSA+DF P VGWIID  +G     +KSF +L
Sbjct: 186 AFGVSFEGSVLNSDRFNKLVRDTFEMLGSFSASDFIPYVGWIIDKFNGLQGWRKKSFRDL 245

Query: 181 DGYFQHVLDYHIKFRKFSGCDDEENIVDVLLKKAREDVP----KFDADCIKALLMDILLA 240
           D +++ + D H K  K  G +D   +VDVLL+  +E++     K   + IKA+LM+ILL 
Sbjct: 246 DAFYEQIFDLH-KEEKEVGSED---LVDVLLRLEKEEIVVGNGKLTRNHIKAILMNILLG 305

Query: 241 GVETGANTLVWALTELVKNPKVMKKLQDEIRSCIK-EDRVKESDLEKLKYLKVVMKEVLR 300
           G++T A T+ WA+ EL KNP+VMKK+Q EIR+ IK ++R+   D +KL+YLK+V+KE  R
Sbjct: 306 GIDTSAITMTWAMAELAKNPRVMKKVQAEIRNQIKNKERISFDDTDKLEYLKMVIKETWR 365

Query: 301 LHALAPLLLPRETMSHFKLNGYDIDPKTLVYINVWAIGRDPKLWTNPEEFLPERFIGSGI 360
           LH   PLLLPR+ ++ F++NGY I  KT +++NVWAIGRDP  W +PE FLPERF  S I
Sbjct: 366 LHPPTPLLLPRDVITEFEINGYTIPAKTRLHVNVWAIGRDPDTWKDPEMFLPERFNDSNI 425

Query: 361 DYKRHNFELLSFGSGRRICPEMNMATIMMELTLANMLLCFDWKLPANMKSKDVGVEEDSS 420
           D K  NFELLSFGSGRRICP + M T M+E  LANML  FDWKLP  M  +D+ +EE   
Sbjct: 426 DAKGQNFELLSFGSGRRICPGLYMGTTMVEFGLANMLYHFDWKLPEGMVVEDIDMEEAPG 485

Query: 421 FVVAKKSPLQLVPICYFN 434
             V+KKS L LVP+ Y +
Sbjct: 486 LTVSKKSELVLVPVKYLD 499

[Close]

BLAST of Lsi04G006220 vs. TrEMBL
Match: W9S1E6_9ROSA (Cytochrome P450 OS=Morus notabilis GN=L484_027664 PE=3 SV=1)

HSP 1 Score: 479.2 bits (1232), Expect = 5.4e-132
Identity = 232/436 (53.21%), Postives = 315/436 (72.25%), Query Frame = 1


Query: 1   MLLKLGSVPTLVISSSAAAKEILKVNDLSCCSRPRLTAGGRFSYKYLDIVLGQYGDHWRE 60
           M L+LG VP +VISS+ AAKE+LK++DL CCSRP L   G+ SY +LD+    YG+HWRE
Sbjct: 65  MRLQLGGVPAIVISSAEAAKEVLKIHDLECCSRPPLAGPGKLSYNFLDVGFSPYGEHWRE 124

Query: 61  VRKICVVGLFNARRVQSFQMIRQEEIDVLLNSISQSSSSATSINLSEKFYSFTINMTTRI 120
           +RK+CVV LF+ + VQSF+ +R+EEI  L++S   +S + T ++LS+K ++ T ++T RI
Sbjct: 125 LRKVCVVRLFSLKSVQSFRFVREEEIGSLIDSFRGNSFTKTPVDLSKKMFALTASVTLRI 184

Query: 121 AFGKKFRGGEIDNDNIENVIRRAVVVLKSFSATDFFPRVGWIIDWLSGFHTRMEKSFTEL 180
           AFGKKF    +DND  E ++ RA  VL  F+A+DFFP VGW +D L+G H ++E+SF EL
Sbjct: 185 AFGKKFEETGLDNDEFEEIMHRAQSVLGGFTASDFFPFVGWFVDRLTGLHAKLERSFHEL 244

Query: 181 DGYFQHVLDYHIKFRKFSGCDDEENIVDVLLKKAREDVP----KFDADCIKALLMDILLA 240
           D +FQ V+D H+     +   ++E++VD++L+  R+       +F  DC KA+LMDI LA
Sbjct: 245 DDFFQRVIDEHL---NRTTTQEQEDVVDLMLRMERDQSEFGEVQFTRDCTKAILMDIFLA 304

Query: 241 GVETGANTLVWALTELVKNPKVMKKLQDEIRSCIKE-DRVKESDLEKLKYLKVVMKEVLR 300
           GV+TGA T+ WA+ EL +NP+VM+K QDEIR+ +K+ + V ES++ +L YLK+V+KE +R
Sbjct: 305 GVDTGAVTITWAMAELARNPRVMRKAQDEIRTRVKKGELVSESEIHQLDYLKMVVKETMR 364

Query: 301 LHALAPLLLPRETMSHFKLNGYDIDPKTLVYINVWAIGRDPKLWTNPEEFLPERFIGSGI 360
           LH  APLLLPRETMS FKL GYD+ PKTL+ +NVWAIGRDP  W NPEEF+PERF    I
Sbjct: 365 LHPAAPLLLPRETMSQFKLFGYDVYPKTLLQVNVWAIGRDPNYWDNPEEFIPERFADGSI 424

Query: 361 DYKRHNFELLSFGSGRRICPEMNMATIMMELTLANMLLCFDWKLPANMKSKDVGVEEDSS 420
           DYK  +FE L FG+GRR CP + M   M+ELTLAN+L CFDWKLP  M+  D+ ++E S 
Sbjct: 425 DYKGQHFEFLPFGAGRRGCPGIYMGIAMVELTLANLLCCFDWKLPDGMEETDIDMDEASG 484

Query: 421 FVVAKKSPLQLVPICY 432
             + KK PL+LVPI Y
Sbjct: 485 LAIHKKFPLKLVPISY 497

[Close]

BLAST of Lsi04G006220 vs. TrEMBL
Match: B9SB66_RICCO (Cytochrome P450, putative OS=Ricinus communis GN=RCOM_0785370 PE=3 SV=1)

HSP 1 Score: 478.4 bits (1230), Expect = 9.2e-132
Identity = 237/437 (54.23%), Postives = 317/437 (72.54%), Query Frame = 1


Query: 1   MLLKLGSVPTLVISSSAAAKEILKVNDLSCCSRPRLTAGGRFSYKYLDIVLGQYGDHWRE 60
           ML+KLG VPT++ISSS AAKE+ K +DL+ CSRP LT  G+ SY YLDI    YGD+WR+
Sbjct: 68  MLVKLGRVPTVIISSSGAAKELFKTHDLNSCSRPLLTGSGKLSYNYLDIAFTPYGDYWRD 127

Query: 61  VRKICVVGLFNARRVQSFQMIRQEEIDVLLNSISQSSSSATSINLSEKFYSFTINMTTRI 120
           +RK+CV+ LF+A+RVQSFQ IR++E+ +L++SIS+SSSS   ++LSEK  + T N+  R 
Sbjct: 128 MRKLCVLELFSAKRVQSFQFIREQEVSLLIDSISKSSSSCP-VDLSEKAMTLTANIICRA 187

Query: 121 AFGKKFRGGEIDNDNIENVIRRAVVVLKSFSATDFFPRVGWIIDWLSGFHTRMEKSFTEL 180
           AFGK F+   ++++  +  I   + +L SFSA DFFP VGWI+D ++G H R+E++F E 
Sbjct: 188 AFGKSFQERGLNHERFQEAIHEGLAMLGSFSAADFFPYVGWIVDRITGLHARLERNFQEF 247

Query: 181 DGYFQHVLDYHIKFRKFSGCDDEENIVDVLL---KKAREDVP--KFDADCIKALLMDILL 240
           D ++Q ++D HI+  +  G    E+I+DVLL   K  RE+    +F  D IKA+LM+I L
Sbjct: 248 DTFYQKIIDDHIQKGRKDGSQQVEDIIDVLLELEKSHREEFGAFQFSKDHIKAILMNIFL 307

Query: 241 AGVETGANTLVWALTELVKNPKVMKKLQDEIRSCIKEDR-VKESDLEKLKYLKVVMKEVL 300
           AGV+TGA TLVWA+TEL++NP+VMKK Q+EIRSCI + R V E D+EK  YLK+V+KE L
Sbjct: 308 AGVDTGAITLVWAMTELIRNPRVMKKAQEEIRSCIGDKRKVSEIDIEKFGYLKIVLKETL 367

Query: 301 RLHALAPLLLPRETMSHFKLNGYDIDPKTLVYINVWAIGRDPKLWTNPEEFLPERFIGSG 360
           R+H  + LL+PRETM+ F +NGYDI PKT + +NVWA+GRDPK+W NP+EF PERF+ S 
Sbjct: 368 RIHPPSVLLIPRETMAQFSINGYDIYPKTRIQVNVWAMGRDPKIWKNPQEFYPERFLDSS 427

Query: 361 IDYKRHNFELLSFGSGRRICPEMNMATIMMELTLANMLLCFDWKLPANMKSKDVGVEEDS 420
           IDYK  N+ELL FG GRR CP + M    +EL LAN+L  FDWKLP NMK +D+ +EE  
Sbjct: 428 IDYKGMNYELLPFGGGRRGCPGITMGMTTVELALANLLFYFDWKLPYNMKIEDINMEEAP 487

Query: 421 SFVVAKKSPLQLVPICY 432
              + KK PL LVP  Y
Sbjct: 488 GLTIHKKEPLLLVPTIY 503

[Close]

BLAST of Lsi04G006220 vs. TrEMBL
Match: B9SB69_RICCO (Cytochrome P450, putative OS=Ricinus communis GN=RCOM_0785500 PE=3 SV=1)

HSP 1 Score: 478.0 bits (1229), Expect = 1.2e-131
Identity = 238/437 (54.46%), Postives = 315/437 (72.08%), Query Frame = 1


Query: 1   MLLKLGSVPTLVISSSAAAKEILKVNDLSCCSRPRLTAGGRFSYKYLDIVLGQYGDHWRE 60
           ML+KLG VPT++ISSS AAKE+ K +DL+ CSRP LT  G+ SY YLDI    YGD+WR+
Sbjct: 68  MLVKLGRVPTVIISSSGAAKELFKTHDLNSCSRPLLTGSGKLSYNYLDIAFTPYGDYWRD 127

Query: 61  VRKICVVGLFNARRVQSFQMIRQEEIDVLLNSISQSSSSATSINLSEKFYSFTINMTTRI 120
           +RK+CV+ LF+A+RVQSFQ IR++E+ +L++SIS+SSSS   ++LSEK  + T N+  R 
Sbjct: 128 MRKLCVLELFSAKRVQSFQFIREQEVSLLIDSISKSSSSCP-VDLSEKAMTLTANIICRA 187

Query: 121 AFGKKFRGGEIDNDNIENVIRRAVVVLKSFSATDFFPRVGWIIDWLSGFHTRMEKSFTEL 180
           AFGK F+   + ++  + VI   +  L SF A DFFP VGWI+D ++G H R+E++F E 
Sbjct: 188 AFGKSFQERGLGHERFQEVIHEGLATLGSFCAADFFPYVGWIVDRITGLHARLERNFQEF 247

Query: 181 DGYFQHVLDYHIKFRKFSGCDDEENIVDVLL---KKAREDVP--KFDADCIKALLMDILL 240
           D ++Q ++D HI+  +  G    E+I+DVLL   K  RE+    +F  D IKA+LM+I L
Sbjct: 248 DTFYQKIIDDHIQKGRKDGSQQVEDIIDVLLELEKSHREEFGAFQFSKDHIKAILMNIFL 307

Query: 241 AGVETGANTLVWALTELVKNPKVMKKLQDEIRSCIKEDR-VKESDLEKLKYLKVVMKEVL 300
           AGV+TGA TLVWA+TEL++NP+VMKK Q+EIRSCI + R V E D+EKL YLK+V+KE L
Sbjct: 308 AGVDTGAITLVWAMTELIRNPRVMKKAQEEIRSCIGDKRKVSEIDIEKLGYLKIVLKETL 367

Query: 301 RLHALAPLLLPRETMSHFKLNGYDIDPKTLVYINVWAIGRDPKLWTNPEEFLPERFIGSG 360
           R+H    LL+PRETM+ F +NGYDI PKT + +NVWA+GRDPK+W NP+EF PERF+ S 
Sbjct: 368 RIHPPGVLLIPRETMAQFSINGYDIYPKTRIQVNVWAMGRDPKIWKNPQEFYPERFLDSS 427

Query: 361 IDYKRHNFELLSFGSGRRICPEMNMATIMMELTLANMLLCFDWKLPANMKSKDVGVEEDS 420
           IDYK  N+ELL FG GRR CP + M    +EL LAN+L  FDWKLP NMK +D+ +EE  
Sbjct: 428 IDYKGMNYELLPFGGGRRGCPGITMGMTTVELALANLLFYFDWKLPYNMKIEDINMEEAP 487

Query: 421 SFVVAKKSPLQLVPICY 432
              + KK PL LVP  Y
Sbjct: 488 GLTIHKKEPLLLVPTIY 503

[Close]

BLAST of Lsi04G006220 vs. TrEMBL
Match: A0A067K4G4_JATCU (Uncharacterized protein OS=Jatropha curcas GN=JCGZ_17832 PE=3 SV=1)

HSP 1 Score: 473.8 bits (1218), Expect = 2.3e-130
Identity = 228/439 (51.94%), Postives = 318/439 (72.44%), Query Frame = 1


Query: 1   MLLKLGSVPTLVISSSAAAKEILKVNDLSCCSRPRLTAGGRFSYKYLDIVLGQYGDHWRE 60
           MLL+LG++P +VISS+ AA+E+LKVNDL+CCSRP+L   GR SY YLD+ L  YGDHWR 
Sbjct: 70  MLLQLGNIPNVVISSAEAAREVLKVNDLACCSRPQLAGAGRLSYNYLDVGLTPYGDHWRN 129

Query: 61  VRKICVVGLFNARRVQSFQMIRQEEIDVLLNSISQSSSSATSINLSEKFYSFTINMTTRI 120
           ++K+ V+ LF+ +RVQSF+ +R+EE+++ ++SIS S++S T INL+EK +S T N+T R+
Sbjct: 130 MKKLIVLQLFSLKRVQSFRSLREEEVELFISSISNSAASETPINLTEKLFSLTANITVRM 189

Query: 121 AFGKKFRGGEIDNDNIENVIRRAVVVLKSFSATDFFPRVGWIIDWLSGFHTRMEKSFTEL 180
           +FG  +RG   D D    V+     V  SFS ++ FP  GW+ DW++  H R E+ F EL
Sbjct: 190 SFGFDYRGTNFDRDRFHEVVHDGEAVAGSFSKSELFPYFGWVFDWITSHHARTERVFHEL 249

Query: 181 DGYFQHVLDYHIKFRKFSGCDDEENIVDVLLK----KAREDVPKFDADCIKALLMDILLA 240
           D +F++ ++ H+K  +    DD   ++DVLL+    +A   + KF  D IK +L+++ LA
Sbjct: 250 DTFFEYAINDHLKPGRNKEKDD---VIDVLLRIKEEQAETGMAKFTNDNIKGVLLNLFLA 309

Query: 241 GVETGANTLVWALTELVKNPKVMKKLQDEIRSCI-KEDRVKESDLEKLKYLKVVMKEVLR 300
           GV+T A T+ WA+ EL +NP VMKK QDE+R+ + K+ R+ E DL++L+YLK+V+KE  R
Sbjct: 310 GVDTSAITVNWAMAELSRNPSVMKKAQDEVRNVVGKKGRITEGDLDQLEYLKMVIKETFR 369

Query: 301 LHALAPLLLPRETMSHFKLNGYDIDPKTLVYINVWAIGRDPKLWTNPEEFLPERFIGSGI 360
           LH  APLLLPRET+SHFK+NGYDI PKTL+ +N WAIGRDPK W +PE+F PERF  S I
Sbjct: 370 LHPAAPLLLPRETISHFKINGYDIYPKTLIQVNAWAIGRDPKYWKDPEQFFPERFADSDI 429

Query: 361 DYKRHNFELLSFGSGRRICPEMNMATIMMELTLANMLLCFDWKLPANMKSKDVGVEEDS- 420
           D+K  NFE L FG+GRRICP ++MATI +E+ LAN+L CFDWKLP  MK +D+ +EE   
Sbjct: 430 DFKGQNFEFLPFGAGRRICPGIHMATITVEIVLANLLYCFDWKLPDGMKREDIDMEEQPG 489

Query: 421 -SFVVAKKSPLQLVPICYF 433
            S  V+KK+PL L+P+ +F
Sbjct: 490 ISLTVSKKTPLNLLPVEHF 505

[Close]

BLAST of Lsi04G006220 vs. TrEMBL
Match: W9RKJ2_9ROSA (Cytochrome P450 OS=Morus notabilis GN=L484_027660 PE=3 SV=1)

HSP 1 Score: 471.1 bits (1211), Expect = 1.5e-129
Identity = 228/438 (52.05%), Postives = 310/438 (70.78%), Query Frame = 1


Query: 1   MLLKLGSVPTLVISSSAAAKEILKVNDLSCCSRPRLTAGGRFSYKYLDIVLGQYGDHWRE 60
           M L+ G VP +++SS+ AAKE+LK+ DL CCSRP L   G  SY  LDI    YG+ WRE
Sbjct: 71  MHLRFGGVPMIIVSSAEAAKEVLKIRDLECCSRPHLVGSGTLSYNNLDISFSPYGERWRE 130

Query: 61  VRKICVVGLFNARRVQSFQMIRQEEIDVLLNSISQSSSSA--TSINLSEKFYSFTINMTT 120
           +RKICV+ LFN ++V+SF+ +R+EEI  L++S+S+SS++   T  NLSE  ++   ++T 
Sbjct: 131 MRKICVLKLFNIKKVKSFRFVREEEISSLIDSLSRSSNNLLQTPANLSEMMFALAASITL 190

Query: 121 RIAFGKKFRGGEIDNDNIENVIRRAVVVLKSFSATDFFPRVGWIIDWLSGFHTRMEKSFT 180
           RIAFGK F    +DND  E  I R    L SF+A+DFFP VGWI+D L+G H+++E  F 
Sbjct: 191 RIAFGKTFGKSGLDNDKFEETIHRVQSALGSFAASDFFPYVGWIVDRLTGLHSKLESVFH 250

Query: 181 ELDGYFQHVLDYHIKFRKFSGCDDEENIVDVLLKKAREDVP----KFDADCIKALLMDIL 240
           ELD +++ V++ H+     +  DD+E+IVD++L+  R+       +F  DC KA++MDI 
Sbjct: 251 ELDNFYERVIEEHLNR---TTQDDQEDIVDLMLRIERDQAEFSEVQFTRDCTKAIIMDIF 310

Query: 241 LAGVETGANTLVWALTELVKNPKVMKKLQDEIRSCIKEDR-VKESDLEKLKYLKVVMKEV 300
           LAGV+TGA T++W + ELV+NP+VM+K QDEIRSC+K+ + V ESD+ +L YL++V+KE 
Sbjct: 311 LAGVDTGAITIIWVMAELVRNPRVMRKAQDEIRSCVKKGKLVSESDIHQLHYLRMVIKET 370

Query: 301 LRLHALAPLLLPRETMSHFKLNGYDIDPKTLVYINVWAIGRDPKLWTNPEEFLPERFIGS 360
           LRLH   PLLLPRE MS FKL GYD+ PKTL+ +NVWAIGRDP  W NPEEF PERF   
Sbjct: 371 LRLHPAIPLLLPREAMSQFKLFGYDVYPKTLLQVNVWAIGRDPNYWHNPEEFFPERFSDG 430

Query: 361 GIDYKRHNFELLSFGSGRRICPEMNMATIMMELTLANMLLCFDWKLPANMKSKDVGVEED 420
            ID+K  +FE L FG+GRR CP ++M   M+ELTLAN+L CFDWKLP  MK  D+ ++E+
Sbjct: 431 SIDFKGQHFEFLPFGAGRRTCPGIHMGIAMVELTLANLLCCFDWKLPDGMKETDIDMDEE 490

Query: 421 SSFVVAKKSPLQLVPICY 432
           S     KK PL+L+P+ Y
Sbjct: 491 SGLTTHKKFPLKLIPVRY 505

[Close]

BLAST of Lsi04G006220 vs. TAIR10
Match: AT3G26320.1 (AT3G26320.1 cytochrome P450, family 71, subfamily B, polypeptide 36)

HSP 1 Score: 431.8 bits (1109), Expect = 5.0e-121
Identity = 212/438 (48.40%), Postives = 301/438 (68.72%), Query Frame = 1


Query: 1   MLLKLGSVPTLVISSSAAAKEILKVNDLSCCSRPRLTAGGRFSYKYLDIVLGQYGDHWRE 60
           MLLK GS+PT+V+SSS  AK++LK++DL CCSRP L      SY YLDI    + D+W+E
Sbjct: 66  MLLKFGSIPTVVVSSSETAKQVLKIHDLHCCSRPSLAGPRALSYNYLDIAFSPFDDYWKE 125

Query: 61  VRKICVVGLFNARRVQSFQMIRQEEIDVLLNSISQSSSSATSINLSEKFYSFTINMTTRI 120
           +R+ICV  LF+ +RVQSFQ I+++E+  L++S+S+S+S  T +NLSEKF S T+ +T + 
Sbjct: 126 LRRICVQELFSVKRVQSFQPIKEDEVKKLIDSVSESASQGTPVNLSEKFTSLTVRVTCKA 185

Query: 121 AFGKKFRGGEIDNDNIENVIRRAVVVLKSFSATDFFPRVGWIIDWLSGFHTRMEKSFTEL 180
            FG  F+G  +++D  E +I    + L SFSA+D+FP  GWIIDWL+G H + E+S   L
Sbjct: 186 TFGVNFQGTVLNSDRFEKLIHDTYLFLGSFSASDYFPNGGWIIDWLTGLHGQRERSVRAL 245

Query: 181 DGYFQHVLDYHIKFRKFSGCDDEENIVDVLLKKAREDV----PKFDADCIKALLMDILLA 240
           D +++ + D H +  K    +  E+ VD+LL+  +E+      K   + IKA+LM++L+ 
Sbjct: 246 DAFYEQMFDLHKQGNK----EGVEDFVDLLLRLEKEETVIGYGKLTRNHIKAILMNVLIG 305

Query: 241 GVETGANTLVWALTELVKNPKVMKKLQDEIRSCI-KEDRVKESDLEKLKYLKVVMKEVLR 300
           G+ T A T+ WA+TEL++NP+VMKK+Q EIR+ I K+  +   D+++L YLK+V+ E  R
Sbjct: 306 GIGTSAITMTWAMTELMRNPRVMKKVQSEIRNQIGKKSMITLDDIDQLHYLKMVINETWR 365

Query: 301 LHALAPLLLPRETMSHFKLNGYDIDPKTLVYINVWAIGRDPKLWTNPEEFLPERFIGSGI 360
           LH  +P L+PR+ MS F+LN Y I  KT +Y+NVWAIGRDP  W +PEEFLPERF+ S I
Sbjct: 366 LHPPSPFLIPRQVMSEFELNDYVIPVKTRLYVNVWAIGRDPDTWKDPEEFLPERFVNSSI 425

Query: 361 DYKRHNFELLSFGSGRRICPEMNMATIMMELTLANMLLCFDWKLPANMKSKDVGVEEDSS 420
           D K  +FELL FGSGRR+CP M M T M+E  LANML  FDWK+P  M ++D+ +EE   
Sbjct: 426 DAKGQHFELLPFGSGRRMCPAMYMGTTMVEFGLANMLYHFDWKIPVGMVAEDIDLEESPG 485

Query: 421 FVVAKKSPLQLVPICYFN 434
              +KK+ L LVP+ Y +
Sbjct: 486 LNASKKNELVLVPLKYLD 499

[Close]

BLAST of Lsi04G006220 vs. TAIR10
Match: AT3G26330.1 (AT3G26330.1 cytochrome P450, family 71, subfamily B, polypeptide 37)

HSP 1 Score: 426.8 bits (1096), Expect = 1.6e-119
Identity = 215/438 (49.09%), Postives = 297/438 (67.81%), Query Frame = 1


Query: 1   MLLKLGSVPTLVISSSAAAKEILKVNDLSCCSRPRLTAGGRFSYKYLDIVLGQYGDHWRE 60
           MLLK GS+PT+V+SSS  AK+ LK++DL+CCSRP L      SY YLDIV   + D+W+E
Sbjct: 66  MLLKFGSIPTVVVSSSETAKQALKIHDLNCCSRPSLAGPRALSYNYLDIVFSPFNDYWKE 125

Query: 61  VRKICVVGLFNARRVQSFQMIRQEEIDVLLNSISQSSSSATSINLSEKFYSFTINMTTRI 120
           +R++CV  LF+ ++V   Q IR+EE+  L+NS S+S++  T +NLSEK  S T+ +  + 
Sbjct: 126 LRRMCVQELFSPKQVHLIQPIREEEVKKLMNSFSESAAQKTPVNLSEKLASLTVGVICKA 185

Query: 121 AFGKKFRGGEIDNDNIENVIRRAVVVLKSFSATDFFPRVGWIIDWLSGFHTRMEKSFTEL 180
           AFG  F+G  +++DN + +I  A + L SFSA+D+FP VGWIIDWL+G   + E+S   L
Sbjct: 186 AFGVSFQGTVLNSDNFDKLIHDAFLFLGSFSASDYFPNVGWIIDWLTGLQGQRERSVRGL 245

Query: 181 DGYFQHVLDYHIKFRKFSGCDDEENIVDVLLKKAREDV----PKFDADCIKALLMDILLA 240
           D +++ + D H +  K    +  E+ VD+LLK  +E+      K   + IKA+LM++LL 
Sbjct: 246 DAFYEQMFDLHKQGNK----EGVEDFVDLLLKLEKEETVLGYGKLTRNHIKAVLMNVLLG 305

Query: 241 GVETGANTLVWALTELVKNPKVMKKLQDEIRSCI-KEDRVKESDLEKLKYLKVVMKEVLR 300
           G+ T A T+ WA+TEL++NP+VMKK+Q EIR+ I  +  +   D+++L YLK+V+ E  R
Sbjct: 306 GIGTSAITMTWAMTELMRNPRVMKKVQSEIRNQIGGKSMICLDDIDQLHYLKMVINETWR 365

Query: 301 LHALAPLLLPRETMSHFKLNGYDIDPKTLVYINVWAIGRDPKLWTNPEEFLPERFIGSGI 360
           LH  APLL+PRE MS F++NGY I  KT +Y+NVW IGRDP  W +PEEFLPERF+ S I
Sbjct: 366 LHPPAPLLVPREVMSEFEINGYTIPAKTRLYVNVWGIGRDPDTWKDPEEFLPERFVNSNI 425

Query: 361 DYKRHNFELLSFGSGRRICPEMNMATIMMELTLANMLLCFDWKLPANMKSKDVGVEEDSS 420
           D K  NFELL FGSGRR+CP M M T M+E  LAN+L  FDWKLP  M  +D+ +EE   
Sbjct: 426 DAKGQNFELLPFGSGRRMCPAMYMGTTMVEFGLANLLYHFDWKLPEGMVVEDIDMEESPG 485

Query: 421 FVVAKKSPLQLVPICYFN 434
              +KK+ L LVP  Y N
Sbjct: 486 LNASKKNELVLVPRKYLN 499

[Close]

BLAST of Lsi04G006220 vs. TAIR10
Match: AT1G13080.1 (AT1G13080.1 cytochrome P450, family 71, subfamily B, polypeptide 2)

HSP 1 Score: 424.9 bits (1091), Expect = 6.1e-119
Identity = 203/434 (46.77%), Postives = 297/434 (68.43%), Query Frame = 1


Query: 1   MLLKLGSVPTLVISSSAAAKEILKVNDLSCCSRPRLTAGGRFSYKYLDIVLGQYGDHWRE 60
           + L+LGSVP +VISSS AA+ +LK NDL CCSRP+    G+ SY + DI    YG++WRE
Sbjct: 66  VFLRLGSVPVVVISSSEAAEAVLKTNDLECCSRPKTVGSGKLSYGFKDITFAPYGEYWRE 125

Query: 61  VRKICVVGLFNARRVQSFQMIRQEEIDVLLNSISQSSSSATSINLSEKFYSFTINMTTRI 120
           VRK+ V+ LF++++VQSF+ IR+EE+D ++  +S+S+   + ++LS+ F+S T ++  R+
Sbjct: 126 VRKLAVIELFSSKKVQSFRYIREEEVDFVVKKVSESALKQSPVDLSKTFFSLTASIICRV 185

Query: 121 AFGKKFR--GGEIDNDNIENVIRRAVVVLKSFSATDFFPR-VGWIIDWLSGFHTRMEKSF 180
           A G+ F   G  ID D IE ++  +   L +F+ +DFFP  +G  +DWL   H ++ K F
Sbjct: 186 ALGQNFNESGFVIDQDRIEELVTESAEALGTFTFSDFFPGGLGRFVDWLFQRHKKINKVF 245

Query: 181 TELDGYFQHVLDYHIKFRKFSGCDDEENIVDVLLKKAREDVPKFDADCIKALLMDILLAG 240
            ELD ++QHV+D H+K       D    I+D++ K+   D  K + D +KA++MD+ LAG
Sbjct: 246 KELDAFYQHVIDDHLKPEGRKNQDIVTLILDMIDKQEDSDSFKLNMDNLKAIVMDVFLAG 305

Query: 241 VETGANTLVWALTELVKNPKVMKKLQDEIRSCI--KEDRVKESDLEKLKYLKVVMKEVLR 300
           ++T A T++WA+TEL++NP+VMKK Q+ IR+ +  K++R+ E DL K++YL  ++KE  R
Sbjct: 306 IDTSAVTMIWAMTELIRNPRVMKKAQESIRTTLGLKKERITEEDLGKVEYLNHILKETFR 365

Query: 301 LHALAPLLLPRETMSHFKLNGYDIDPKTLVYINVWAIGRDPKLWTNPEEFLPERFIGSGI 360
           LH   P ++PRETMSH K+ GYDI PKT + +NVW IGRDPK W +PEEF PERF  S +
Sbjct: 366 LHPALPFVVPRETMSHIKIQGYDIPPKTQIQLNVWTIGRDPKRWNDPEEFNPERFANSSV 425

Query: 361 DYKRHNFELLSFGSGRRICPEMNMATIMMELTLANMLLCFDWKLPANMKSKDVGVEEDSS 420
           D++  +F+LL FGSGRRICP M MA   +EL L N+L  FDW +P   K +D+ +EE  +
Sbjct: 426 DFRGQHFDLLPFGSGRRICPGMPMAIASVELALMNLLYYFDWSMPDGTKGEDIDMEEAGN 485

Query: 421 FVVAKKSPLQLVPI 430
             + KK PLQLVP+
Sbjct: 486 ISIVKKIPLQLVPV 499

[Close]

BLAST of Lsi04G006220 vs. TAIR10
Match: AT2G02580.1 (AT2G02580.1 cytochrome P450, family 71, subfamily B, polypeptide 9)

HSP 1 Score: 423.7 bits (1088), Expect = 1.4e-118
Identity = 214/436 (49.08%), Postives = 291/436 (66.74%), Query Frame = 1


Query: 1   MLLKLGSVPTLVISSSAAAKEILKVNDLSCCSRPRLTAGGRFSYKYLDIVLGQYGDHWRE 60
           MLLKLGSVPT+V+SSS  AK++LK+NDL CCSRP L      SY YLDI    + D+W+E
Sbjct: 66  MLLKLGSVPTVVVSSSETAKQVLKINDLHCCSRPSLAGAKELSYNYLDIAFSPFDDYWKE 125

Query: 61  VRKICVVGLFNARRVQSFQMIRQEEIDVLLNSISQSSSSATSINLSEKFYSFTINMTTRI 120
           +R+ICV  LF+A+RV S Q I++EE+  L+ S ++S+S  + +NLSEKF   T+++  + 
Sbjct: 126 LRRICVQELFSAKRVHSIQPIKEEEVRKLIVSATESASQKSPVNLSEKFLDLTVSVICKA 185

Query: 121 AFGKKFRGGEIDNDNIENVIRRAVVVLKSFSATDFFPRVGWIIDWLSGFHTRMEKSFTEL 180
           AF   F    ++ND  + +I  A + L SFSA++FFP  GWIIDWL+G   R EKS  +L
Sbjct: 186 AFSLDFHTSVLNNDGFDKLIHDAFLFLGSFSASNFFPNGGWIIDWLTGLQRRREKSVKDL 245

Query: 181 DGYFQHVLDYHIKFRKFSGCDDEENIVDVLLKKAREDV----PKFDADCIKALLMDILLA 240
           D ++Q + D H +  K       E+ VD+LLK  +E+      K   + +KA+LM++LL 
Sbjct: 246 DVFYQQMFDLHKQENK----QGVEDFVDLLLKLEKEETVLGYGKLTRNHVKAILMNVLLG 305

Query: 241 GVETGANTLVWALTELVKNPKVMKKLQDEIRS-CIKEDRVKESDLEKLKYLKVVMKEVLR 300
            + T A T+ WA+ EL++NP+VMKK+Q EIR+  I +  +   D++ L YLK+V+KE  R
Sbjct: 306 AINTSAMTMTWAMAELIRNPRVMKKVQSEIRNQMINKSVITLDDIDHLPYLKMVIKETWR 365

Query: 301 LHALAPLLLPRETMSHFKLNGYDIDPKTLVYINVWAIGRDPKLWTNPEEFLPERFIGSGI 360
           LH   PLLLPRE MS F++NGY I PKTL+Y+NVWAIGRDP  W + + F PERF+ + I
Sbjct: 366 LHPPVPLLLPREVMSEFEINGYKIQPKTLLYVNVWAIGRDPDSWKDADMFYPERFMDNNI 425

Query: 361 DYKRHNFELLSFGSGRRICPEMNMATIMMELTLANMLLCFDWKLPANMKSKDVGVEEDSS 420
           D K  NFELL FGSGRRICP M M T M+E  LANML  FDW++P  M  +D+ +EE   
Sbjct: 426 DAKGQNFELLPFGSGRRICPGMYMGTTMVEFGLANMLYQFDWEVPDGMVVEDIDMEESPG 485

Query: 421 FVVAKKSPLQLVPICY 432
             V KK+ L LVP+ Y
Sbjct: 486 LAVGKKNELLLVPVKY 497

[Close]

BLAST of Lsi04G006220 vs. TAIR10
Match: AT3G26290.1 (AT3G26290.1 cytochrome P450, family 71, subfamily B, polypeptide 26)

HSP 1 Score: 414.5 bits (1064), Expect = 8.3e-116
Identity = 214/438 (48.86%), Postives = 294/438 (67.12%), Query Frame = 1


Query: 1   MLLKLGSVPTLVISSSAAAKEILKVNDLSCCSRPRLTAGGRFSYKYLDIVLGQYGDHWRE 60
           MLLKLG VPTL++SSS  AK+ L+  DL CCSRP L  G   SY  LD+    Y ++W+E
Sbjct: 66  MLLKLGKVPTLILSSSETAKQALRDYDLHCCSRPSLAGGRELSYNNLDMSSSPYNEYWKE 125

Query: 61  VRKICVVGLFNARRVQSFQMIRQEEIDVLLNSISQSSSSATSINLSEKFYSFTINMTTRI 120
           +RK+C   LF+A ++QS Q I+ EE+  +++SI++SSS    +NLS+ F + T ++  + 
Sbjct: 126 LRKLCSQELFSANKIQSIQPIKDEEVKKVIDSIAESSSLKNPVNLSKTFLALTTSVVCKA 185

Query: 121 AFGKKFRGGEIDNDNIENVIRRAVVVLKSFSATDFFPRVGWIIDWLSGFHTRMEKSFTEL 180
           AFG  F G  +++D    ++R    +L SFSA+DF P VGWIID  +G     +KSF +L
Sbjct: 186 AFGVSFEGSVLNSDRFNKLVRDTFEMLGSFSASDFIPYVGWIIDKFNGLQGWRKKSFRDL 245

Query: 181 DGYFQHVLDYHIKFRKFSGCDDEENIVDVLLKKAREDVP----KFDADCIKALLMDILLA 240
           D +++ + D H K  K  G +D   +VDVLL+  +E++     K   + IKA+LM+ILL 
Sbjct: 246 DAFYEQIFDLH-KEEKEVGSED---LVDVLLRLEKEEIVVGNGKLTRNHIKAILMNILLG 305

Query: 241 GVETGANTLVWALTELVKNPKVMKKLQDEIRSCIK-EDRVKESDLEKLKYLKVVMKEVLR 300
           G++T A T+ WA+ EL KNP+VMKK+Q EIR+ IK ++R+   D +KL+YLK+V+KE  R
Sbjct: 306 GIDTSAITMTWAMAELAKNPRVMKKVQAEIRNQIKNKERISFDDTDKLEYLKMVIKETWR 365

Query: 301 LHALAPLLLPRETMSHFKLNGYDIDPKTLVYINVWAIGRDPKLWTNPEEFLPERFIGSGI 360
           LH   PLLLPR+ ++ F++NGY I  KT +++NVWAIGRDP  W +PE FLPERF  S I
Sbjct: 366 LHPPTPLLLPRDVITEFEINGYTIPAKTRLHVNVWAIGRDPDTWKDPEMFLPERFNDSNI 425

Query: 361 DYKRHNFELLSFGSGRRICPEMNMATIMMELTLANMLLCFDWKLPANMKSKDVGVEEDSS 420
           D K  NFELLSFGSGRRICP + M T M+E  LANML  FDWKLP  M  +D+ +EE   
Sbjct: 426 DAKGQNFELLSFGSGRRICPGLYMGTTMVEFGLANMLYHFDWKLPEGMVVEDIDMEEAPG 485

Query: 421 FVVAKKSPLQLVPICYFN 434
             V+KKS L LVP+ Y +
Sbjct: 486 LTVSKKSELVLVPVKYLD 499

[Close]

BLAST of Lsi04G006220 vs. NCBI nr
Match: gi|449435434|ref|XP_004135500.1| (PREDICTED: cytochrome P450 71B37-like [Cucumis sativus])

HSP 1 Score: 598.6 bits (1542), Expect = 8.8e-168
Identity = 301/448 (67.19%), Postives = 356/448 (79.46%), Query Frame = 1


Query: 1   MLLKLGSVPTLVISSSAAAKEILKVNDLSCCSRPRLTAGGRFSYKYLDIVLGQYGDHWRE 60
           MLLKLGS+PT+V+SS+ AAKE+LKV+DL+ CSRPR TA  RFSY YLDI    YGDHWR+
Sbjct: 44  MLLKLGSIPTVVVSSATAAKEVLKVHDLASCSRPRSTANARFSYNYLDIGFAPYGDHWRK 103

Query: 61  VRKICVVGLFNARRVQSFQMIRQEEIDVLLNSISQSSS--SATSINLSEKFYSFTINMTT 120
           VRKI V+ LF+ARRVQSFQ IR+EEI V+LNSISQSS   S   ++LSEKFYS T NM T
Sbjct: 104 VRKISVLELFSARRVQSFQNIREEEIGVMLNSISQSSLLLSNDPVDLSEKFYSLTANMIT 163

Query: 121 RIAFGKKFRGGEIDNDNIENVIRRAVVVLKSFSATDFFPRVGWIIDWLSGFHTRMEKSFT 180
           RIAFGKKFRGGE+DN+N + VIRRA+  + SFSA D+ P VGWIIDW+SG H+R+E SF 
Sbjct: 164 RIAFGKKFRGGELDNENFQKVIRRAMAAVGSFSAADYLPIVGWIIDWVSGVHSRLETSFN 223

Query: 181 ELDGYFQHVLDYHIKFRKFSGCD-------DEENIVDVLLKKAREDVP----KFDADCIK 240
           ELD +FQH++D  I FR+            D+ENIVDVLLK  +        K   DCIK
Sbjct: 224 ELDAFFQHIVDDRINFRESCSSHNNNGDGYDQENIVDVLLKMEKNSSQYGEVKLTRDCIK 283

Query: 241 ALLMDILLAGVETGANTLVWALTELVKNPKVMKKLQDEIRSCIKEDR-VKESDLEKLKYL 300
           AL+MDI LAGVETGA+TLVW + EL+KNPKVMKKLQ+EIR+C+KE++ VKE+DL+ L+YL
Sbjct: 284 ALIMDIFLAGVETGASTLVWTMAELIKNPKVMKKLQNEIRNCVKENKMVKENDLQNLEYL 343

Query: 301 KVVMKEVLRLHALAPLLLPRETMSHFKLNGYDIDPKTLVYINVWAIGRDPKLWTNPEEFL 360
           K V+KEVLRLH  APLLLPRE MSHFKLNGYDI PKT +Y+N WAIGRDP++WTNPEEF+
Sbjct: 344 KAVVKEVLRLHTPAPLLLPREAMSHFKLNGYDILPKTHIYVNAWAIGRDPEIWTNPEEFI 403

Query: 361 PERFIGSGIDYKRHNFELLSFGSGRRICPEMNMATIMMELTLANMLLCFDWKLPANMKSK 420
           PERFIGS IDYK  NFELL FGSGRRICP MNMA+  +EL LAN+LLCFDWKL   MK +
Sbjct: 404 PERFIGSNIDYKGQNFELLPFGSGRRICPGMNMASFTVELALANVLLCFDWKLANGMKEE 463

Query: 421 DVGVEEDSSFVVAKKSPLQLVPICYFNS 435
           DV +EE++   VAKKSPLQLVP+ YFNS
Sbjct: 464 DVDMEEETGLAVAKKSPLQLVPVHYFNS 491

[Close]

BLAST of Lsi04G006220 vs. NCBI nr
Match: gi|659092302|ref|XP_008447001.1| (PREDICTED: cytochrome P450 71B34-like [Cucumis melo])

HSP 1 Score: 554.7 bits (1428), Expect = 1.5e-154
Identity = 272/435 (62.53%), Postives = 344/435 (79.08%), Query Frame = 1


Query: 1   MLLKLGSVPTLVISSSAAAKEILKVNDLSCCSRPRLTAGGRFSYKYLDIVLGQYGDHWRE 60
           MLLKLGSVPT+ ISS++AA+E+ + +DL+ CSRPRLT  GRFSY + D+ L  YG+ WRE
Sbjct: 73  MLLKLGSVPTIAISSASAARELFRQHDLASCSRPRLTGSGRFSYNFQDLNLSPYGERWRE 132

Query: 61  VRKICVVGLFNARRVQSFQMIRQEEIDVLLNSISQSSSSATSINLSEKFYSFTINMTTRI 120
           +RKI ++ LF+ +RVQSF  IR+EEID LLN IS SSS  T I+L++  Y+ T N+T RI
Sbjct: 133 LRKIFILELFSTKRVQSFHHIREEEIDKLLNCISNSSSLGTPIDLAQTSYALTANVTFRI 192

Query: 121 AFGKKFRGGEIDNDNIENVIRRAVVVLKSFSATDFFPRVGWIIDWLSGFHTRMEKSFTEL 180
           AFGK+F GGE+DN+N +++IRR++V L SF ATD+FP VGWI+DW+SG +  +EKSF E+
Sbjct: 193 AFGKRFSGGELDNENFQHIIRRSMVALGSFFATDYFPCVGWIVDWISGVNGTLEKSFGEM 252

Query: 181 DGYFQHVLDYHIKFRKFSGCDDEENIVDVLLKKARED----VPKFDADCIKALLMDILLA 240
           D  FQ V+D  IKF++ S    EENIVDVLL+  R+       KF  +C+KAL+MDI LA
Sbjct: 253 DAIFQKVVDDRIKFKE-SCRSSEENIVDVLLRMERDSSELHAVKFTHECVKALIMDIFLA 312

Query: 241 GVETGANTLVWALTELVKNPKVMKKLQDEIRSCIKEDRVKESDLEKLKYLKVVMKEVLRL 300
           GVETG N+++WA+ EL+KNP+VMKKLQDEIRS IKEDRVKESDLEKL+YLK+V+KEVLRL
Sbjct: 313 GVETGENSIIWAMAELIKNPRVMKKLQDEIRSTIKEDRVKESDLEKLQYLKMVVKEVLRL 372

Query: 301 HALAPLLLPRETMSHFKLNGYDIDPKTLVYINVWAIGRDPKLWTNPEEFLPERFIGSGID 360
           H   PLLLPRE+MSHFKLNGYDI PKT +++N WAIGRDP+ W +P +F+PERFI + ID
Sbjct: 373 HPPVPLLLPRESMSHFKLNGYDIHPKTHIHVNAWAIGRDPESWKHPLQFIPERFIENNID 432

Query: 361 YKRHNFELLSFGSGRRICPEMNMATIMMELTLANMLLCFDWKLPANMKSKDVGVEEDSSF 420
           YK  +FEL+ FG+GRRICP MNM  I++EL LANMLLCFDWKLP  MK +DV +EED+  
Sbjct: 433 YKGQHFELIPFGAGRRICPGMNMGIIIVELALANMLLCFDWKLPNGMKEEDVDMEEDAGL 492

Query: 421 VVAKKSPLQLVPICY 432
             +KK PLQL+PI Y
Sbjct: 493 SASKKLPLQLIPIRY 506

[Close]

BLAST of Lsi04G006220 vs. NCBI nr
Match: gi|778704858|ref|XP_004135496.2| (PREDICTED: cytochrome P450 71B26-like isoform X2 [Cucumis sativus])

HSP 1 Score: 542.3 bits (1396), Expect = 7.5e-151
Identity = 270/437 (61.78%), Postives = 336/437 (76.89%), Query Frame = 1


Query: 1   MLLKLGSVPTLVISSSAAAKEILKVNDLSCCSRPRLTAGGRFSYKYLDIVLGQYGDHWRE 60
           MLLKLGSVPT+ ISS+ AA+E+ K +DL+ CSRPRLT  GRFSY + D+ L  +G+ WRE
Sbjct: 74  MLLKLGSVPTIAISSATAARELFKHHDLASCSRPRLTGTGRFSYNFQDLNLSPHGERWRE 133

Query: 61  VRKICVVGLFNARRVQSFQMIRQEEIDVLLNSISQSSSSATSINLSEKFYSFTINMTTRI 120
           +RKI +  LF+ +RVQSF  IR+EEID LL  IS SSS  T I+L++  Y+ T N+  R+
Sbjct: 134 LRKIFMTELFSTKRVQSFYHIREEEIDKLLKFISNSSSLGTPIDLAKTSYALTANVIFRM 193

Query: 121 AFGKKFRGGEIDNDNIENVIRRAVVVLKSFSATDFFPRVGWIIDWLSGFHTRMEKSFTEL 180
           AFGKKF GGE+DN+N +++IRR++V L SF  +DFFPRVGW++DW+SG +  +EKSF E+
Sbjct: 194 AFGKKFSGGELDNENFQHIIRRSMVALGSFFVSDFFPRVGWLVDWISGAYGTLEKSFGEM 253

Query: 181 DGYFQHVLDYHIKFRKFSGCDDEENIVDVLLKKARE----DVPKFDADCIKALLMDILLA 240
           D  FQ V+D  IKF++ S    EENIVDVLL+  R+    D  KF  +C+KAL+MDI LA
Sbjct: 254 DAIFQKVVDDRIKFKE-SYRSSEENIVDVLLRMERDSSEFDTVKFTHECVKALIMDIFLA 313

Query: 241 GVETGANTLVWALTELVKNPKVMKKLQDEIRSCIKEDRVKESDLEKLKYLKVVMKEVLRL 300
           GVETG N++VWA+TEL+KNP+VMKKLQDEIRS IKEDRVKESDL+ L+YL +V+KEVLRL
Sbjct: 314 GVETGENSIVWAMTELIKNPRVMKKLQDEIRSTIKEDRVKESDLQNLQYLNMVIKEVLRL 373

Query: 301 HALAPLLLPRETMSHFKLNGYDIDPKTLVYINVWAIGRDPKLWTNPEEFLPERFIGSGID 360
           H   PLLLPRE  SHFKLNGYDI PKT +Y+NVWAIGRD + W NP EF PERFI S ID
Sbjct: 374 HPPVPLLLPREATSHFKLNGYDIHPKTHIYVNVWAIGRDRESWKNPLEFFPERFIESNID 433

Query: 361 YKRHNFELLSFGSGRRICPEMNMATIMMELTLANMLLCFDWKLPANMKSKDVGVEEDSSF 420
           YK  NFEL+ FG+GRRIC  M M  I++EL LANMLLCFDWKLP  +K +DV +EED+  
Sbjct: 434 YKGQNFELIPFGAGRRICAGMTMGIIIVELALANMLLCFDWKLPKGVKEEDVDMEEDAGL 493

Query: 421 VVAKKSPLQLVPICYFN 434
             +KK PLQL+PI Y +
Sbjct: 494 SASKKLPLQLIPIPYLS 509

[Close]

BLAST of Lsi04G006220 vs. NCBI nr
Match: gi|778704862|ref|XP_011655596.1| (PREDICTED: cytochrome P450 71B2-like isoform X3 [Cucumis sativus])

HSP 1 Score: 542.3 bits (1396), Expect = 7.5e-151
Identity = 270/437 (61.78%), Postives = 336/437 (76.89%), Query Frame = 1


Query: 1   MLLKLGSVPTLVISSSAAAKEILKVNDLSCCSRPRLTAGGRFSYKYLDIVLGQYGDHWRE 60
           MLLKLGSVPT+ ISS+ AA+E+ K +DL+ CSRPRLT  GRFSY + D+ L  +G+ WRE
Sbjct: 74  MLLKLGSVPTIAISSATAARELFKHHDLASCSRPRLTGTGRFSYNFQDLNLSPHGERWRE 133

Query: 61  VRKICVVGLFNARRVQSFQMIRQEEIDVLLNSISQSSSSATSINLSEKFYSFTINMTTRI 120
           +RKI +  LF+ +RVQSF  IR+EEID LL  IS SSS  T I+L++  Y+ T N+  R+
Sbjct: 134 LRKIFMTELFSTKRVQSFYHIREEEIDKLLKFISNSSSLGTPIDLAKTSYALTANVIFRM 193

Query: 121 AFGKKFRGGEIDNDNIENVIRRAVVVLKSFSATDFFPRVGWIIDWLSGFHTRMEKSFTEL 180
           AFGKKF GGE+DN+N +++IRR++V L SF  +DFFPRVGW++DW+SG +  +EKSF E+
Sbjct: 194 AFGKKFSGGELDNENFQHIIRRSMVALGSFFVSDFFPRVGWLVDWISGAYGTLEKSFGEM 253

Query: 181 DGYFQHVLDYHIKFRKFSGCDDEENIVDVLLKKARE----DVPKFDADCIKALLMDILLA 240
           D  FQ V+D  IKF++ S    EENIVDVLL+  R+    D  KF  +C+KAL+MDI LA
Sbjct: 254 DAIFQKVVDDRIKFKE-SYRSSEENIVDVLLRMERDSSEFDTVKFTHECVKALIMDIFLA 313

Query: 241 GVETGANTLVWALTELVKNPKVMKKLQDEIRSCIKEDRVKESDLEKLKYLKVVMKEVLRL 300
           GVETG N++VWA+TEL+KNP+VMKKLQDEIRS IKEDRVKESDL+ L+YL +V+KEVLRL
Sbjct: 314 GVETGENSIVWAMTELIKNPRVMKKLQDEIRSTIKEDRVKESDLQNLQYLNMVIKEVLRL 373

Query: 301 HALAPLLLPRETMSHFKLNGYDIDPKTLVYINVWAIGRDPKLWTNPEEFLPERFIGSGID 360
           H   PLLLPRE  SHFKLNGYDI PKT +Y+NVWAIGRD + W NP EF PERFI S ID
Sbjct: 374 HPPVPLLLPREATSHFKLNGYDIHPKTHIYVNVWAIGRDRESWKNPLEFFPERFIESNID 433

Query: 361 YKRHNFELLSFGSGRRICPEMNMATIMMELTLANMLLCFDWKLPANMKSKDVGVEEDSSF 420
           YK  NFEL+ FG+GRRIC  M M  I++EL LANMLLCFDWKLP  +K +DV +EED+  
Sbjct: 434 YKGQNFELIPFGAGRRICAGMTMGIIIVELALANMLLCFDWKLPKGVKEEDVDMEEDAGL 493

Query: 421 VVAKKSPLQLVPICYFN 434
             +KK PLQL+PI Y +
Sbjct: 494 SASKKLPLQLIPIPYLS 509

[Close]

BLAST of Lsi04G006220 vs. NCBI nr
Match: gi|778704855|ref|XP_004135281.2| (PREDICTED: cytochrome P450 71B2-like isoform X1 [Cucumis sativus])

HSP 1 Score: 528.5 bits (1360), Expect = 1.1e-146
Identity = 264/438 (60.27%), Postives = 334/438 (76.26%), Query Frame = 1


Query: 1   MLLKLGSVPTLVISSSAAAKEILKVNDLSCCSRPRLTAGGRFSYKYLDIVLGQYGDHWRE 60
           MLLKLGS+PT+VISS+ AA+E+ K +DL+ CSRPRLT  GRFSY + D+ L  YG  WRE
Sbjct: 74  MLLKLGSIPTVVISSATAARELFKHHDLASCSRPRLTGSGRFSYNFQDLNLSPYGVRWRE 133

Query: 61  VRKICVVGLFNARRVQSFQMIRQEEIDVLLNSISQSSS--SATSINLSEKFYSFTINMTT 120
           +RKI ++ LF+ +RVQSF  IR++E+ +L+NSISQ S   S+  I+L +K YS T N+TT
Sbjct: 134 LRKIFILELFSTKRVQSFHHIREKEVSLLINSISQQSLKFSSNPIDLGDKSYSLTANITT 193

Query: 121 RIAFGKKFRGGEIDNDNIENVIRRAVVVLKSFSATDFFPRVGWIIDWLSGFHTRMEKSFT 180
           RIAFGK FRGGE+DN N + V+RR +  +K FS +DFFP  GWI+D +SG H ++EKSF 
Sbjct: 194 RIAFGKSFRGGELDNQNFQKVMRRTIDAIKGFSISDFFPSFGWIVDRISGVHGKLEKSFG 253

Query: 181 ELDGYFQHVLDYHIKFRKFSGCDDEENIVDVLLKKARE----DVPKFDADCIKALLMDIL 240
           E+D +FQ V+D  I   K +   +EENIVDVLL+  R+    D      DCIKA++ DI 
Sbjct: 254 EMDAFFQKVVDDRINMDKATS-GNEENIVDVLLRMKRDGFQSDALILTQDCIKAIIKDIF 313

Query: 241 LAGVETGANTLVWALTELVKNPKVMKKLQDEIRSCIKEDRVKESDLEKLKYLKVVMKEVL 300
           +AGVETGANT+VWA+ EL++ P+VMKKLQD+IRS IK+++VKE+DLE+L YLK+V+KEVL
Sbjct: 314 IAGVETGANTIVWAMAELIRKPRVMKKLQDQIRSYIKKEQVKETDLERLPYLKMVVKEVL 373

Query: 301 RLHALAPLLLPRETMSHFKLNGYDIDPKTLVYINVWAIGRDPKLWTNPEEFLPERFIGSG 360
           RLH   PLL+PRET SHFKLNGYDI PK  +++NVWAIGRDP+ W NPEEF+PERFI + 
Sbjct: 374 RLHPPVPLLIPRETTSHFKLNGYDIHPKAHLHVNVWAIGRDPECWVNPEEFIPERFIENN 433

Query: 361 IDYKRHNFELLSFGSGRRICPEMNMATIMMELTLANMLLCFDWKLPANMKSKDVGVEED- 420
           IDYK  N+ELL FG GRR+CP MNM    +ELTLAN+LLCFDWKL   MK +DV +EED 
Sbjct: 434 IDYKGQNYELLPFGGGRRVCPGMNMGIFTVELTLANLLLCFDWKLGDGMKEEDVDMEEDF 493

Query: 421 SSFVVAKKSPLQLVPICY 432
               VAKKSPL+LVPI Y
Sbjct: 494 FGISVAKKSPLKLVPIPY 510

The following BLAST results are available for this feature:

BLAST of Lsi04G006220 vs. Swiss-Prot


Match Name	E-value	Identity	Description
C71BX_ARATH	8.9e-120	48.40	Cytochrome P450 71B36 OS=Arabidopsis thaliana GN=CYP71B36 PE=3 SV=1	[more]
C71BY_ARATH	2.9e-118	49.09	Cytochrome P450 71B37 OS=Arabidopsis thaliana GN=CYP71B37 PE=3 SV=2	[more]
C71B2_ARATH	1.1e-117	46.77	Cytochrome P450 71B2 OS=Arabidopsis thaliana GN=CYP71B2 PE=2 SV=2	[more]
C71B9_ARATH	2.4e-117	49.08	Cytochrome P450 71B9 OS=Arabidopsis thaliana GN=CYP71B9 PE=2 SV=3	[more]
C71BQ_ARATH	1.5e-114	48.86	Cytochrome P450 71B26 OS=Arabidopsis thaliana GN=CYP71B26 PE=2 SV=1	[more]

BLAST of Lsi04G006220 vs. TrEMBL


Match Name	E-value	Identity	Description
W9S1E6_9ROSA	5.4e-132	53.21	Cytochrome P450 OS=Morus notabilis GN=L484_027664 PE=3 SV=1	[more]
B9SB66_RICCO	9.2e-132	54.23	Cytochrome P450, putative OS=Ricinus communis GN=RCOM_0785370 PE=3 SV=1	[more]
B9SB69_RICCO	1.2e-131	54.46	Cytochrome P450, putative OS=Ricinus communis GN=RCOM_0785500 PE=3 SV=1	[more]
A0A067K4G4_JATCU	2.3e-130	51.94	Uncharacterized protein OS=Jatropha curcas GN=JCGZ_17832 PE=3 SV=1	[more]
W9RKJ2_9ROSA	1.5e-129	52.05	Cytochrome P450 OS=Morus notabilis GN=L484_027660 PE=3 SV=1	[more]

BLAST of Lsi04G006220 vs. TAIR10


Match Name	E-value	Identity	Description
AT3G26320.1	5.0e-121	48.40	cytochrome P450, family 71, subfamily B, polypeptide 36	[more]
AT3G26330.1	1.6e-119	49.09	cytochrome P450, family 71, subfamily B, polypeptide 37	[more]
AT1G13080.1	6.1e-119	46.77	cytochrome P450, family 71, subfamily B, polypeptide 2	[more]
AT2G02580.1	1.4e-118	49.08	cytochrome P450, family 71, subfamily B, polypeptide 9	[more]
AT3G26290.1	8.3e-116	48.86	cytochrome P450, family 71, subfamily B, polypeptide 26	[more]

BLAST of Lsi04G006220 vs. NCBI nr


Match Name	E-value	Identity	Description
gi\|449435434\|ref\|XP_004135500.1\|	8.8e-168	67.19	PREDICTED: cytochrome P450 71B37-like [Cucumis sativus]	[more]
gi\|659092302\|ref\|XP_008447001.1\|	1.5e-154	62.53	PREDICTED: cytochrome P450 71B34-like [Cucumis melo]	[more]
gi\|778704858\|ref\|XP_004135496.2\|	7.5e-151	61.78	PREDICTED: cytochrome P450 71B26-like isoform X2 [Cucumis sativus]	[more]
gi\|778704862\|ref\|XP_011655596.1\|	7.5e-151	61.78	PREDICTED: cytochrome P450 71B2-like isoform X3 [Cucumis sativus]	[more]
gi\|778704855\|ref\|XP_004135281.2\|	1.1e-146	60.27	PREDICTED: cytochrome P450 71B2-like isoform X1 [Cucumis sativus]	[more]

Annotated Terms

The following terms have been associated with this gene:

Vocabulary: **Biological Process**
Term	Definition
GO:0055114	oxidation-reduction process

Vocabulary: **Molecular Function**
Term	Definition
GO:0020037	heme binding
GO:0005506	iron ion binding
GO:0016705	oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen

Vocabulary: **INTERPRO**
Term	Definition
IPR002401	Cyt_P450_E_grp-I
IPR001128	Cyt_P450

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0055114	oxidation-reduction process
cellular_component	GO:0016020	membrane
cellular_component	GO:0005575	cellular_component
molecular_function	GO:0020037	heme binding
molecular_function	GO:0005506	iron ion binding
molecular_function	GO:0004497	monooxygenase activity
molecular_function	GO:0016705	oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen
molecular_function	GO:0016709	oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen, NAD(P)H as one donor, and incorporation of one atom of oxygen

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Lsi04G006220.1	Lsi04G006220.1	mRNA

InterPro

Analysis Name: InterPro Annotations of Lagenaria siceraria
Date Performed: 2017-09-18


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR001128	Cytochrome P450	PRINTS	PR00385	P450	coord: 288..299 score: 4.0E-8coord: 365..374 score: 4.0E-8coord: 236..253 score: 4.
IPR001128	Cytochrome P450	GENE3D	G3DSA:1.10.630.10		coord: 2..428 score: 1.4E
IPR001128	Cytochrome P450	PFAM	PF00067	p450	coord: 3..408 score: 1.8
IPR001128	Cytochrome P450	unknown	SSF48264	Cytochrome P450	coord: 2..427 score: 8.91E
IPR002401	Cytochrome P450, E-class, group I	PRINTS	PR00463	EP450I	coord: 225..242 score: 1.7E-35coord: 364..374 score: 1.7E-35coord: 374..397 score: 1.7E-35coord: 112..130 score: 1.7E-35coord: 18..39 score: 1.7E-35coord: 245..271 score: 1.7E-35coord: 287..305 score: 1.7E-35coord: 328..352 score: 1.7
None	No IPR available	PANTHER	PTHR24298	FAMILY NOT NAMED	coord: 1..435 score: 7.9E
None	No IPR available	PANTHER	PTHR24298:SF85	CYTOCHROME P450 71B10-RELATED	coord: 1..435 score: 7.9E

Synteny

The following gene(s) are orthologous to this gene:


Gene	Orthologue	Organism	Block
Lsi04G006220	CmoCh18G007420	Cucurbita moschata (Rifu)	cmolsiB368
Lsi04G006220	CmoCh04G018790	Cucurbita moschata (Rifu)	cmolsiB661

The following gene(s) are paralogous to this gene:

None

The following block(s) are covering this gene:


Gene	Organism	Block
Lsi04G006220	Bottle gourd (USVL1VR-Ls)	lsilsiB097
Lsi04G006220	Bottle gourd (USVL1VR-Ls)	lsilsiB149
Lsi04G006220	Cucumber (Gy14) v1	cgylsiB465
Lsi04G006220	Cucumber (Gy14) v1	cgylsiB636
Lsi04G006220	Cucurbita maxima (Rimu)	cmalsiB383
Lsi04G006220	Cucurbita maxima (Rimu)	cmalsiB670
Lsi04G006220	Cucurbita maxima (Rimu)	cmalsiB726
Lsi04G006220	Cucurbita moschata (Rifu)	cmolsiB716
Lsi04G006220	Cucurbita pepo (Zucchini)	cpelsiB030
Lsi04G006220	Cucurbita pepo (Zucchini)	cpelsiB353
Lsi04G006220	Wild cucumber (PI 183967)	cpilsiB116
Lsi04G006220	Wild cucumber (PI 183967)	cpilsiB372
Lsi04G006220	Cucumber (Chinese Long) v2	culsiB119
Lsi04G006220	Cucumber (Chinese Long) v2	culsiB368
Lsi04G006220	Melon (DHL92) v3.5.1	lsimeB281
Lsi04G006220	Melon (DHL92) v3.5.1	lsimeB289
Lsi04G006220	Melon (DHL92) v3.5.1	lsimeB303
Lsi04G006220	Watermelon (Charleston Gray)	lsiwcgB283
Lsi04G006220	Watermelon (Charleston Gray)	lsiwcgB308
Lsi04G006220	Watermelon (97103) v1	lsiwmB296
Lsi04G006220	Watermelon (97103) v1	lsiwmB312
Lsi04G006220	Cucumber (Gy14) v2	cgyblsiB105
Lsi04G006220	Cucumber (Gy14) v2	cgyblsiB337
Lsi04G006220	Melon (DHL92) v3.6.1	lsimedB297
Lsi04G006220	Melon (DHL92) v3.6.1	lsimedB317
Lsi04G006220	Silver-seed gourd	carlsiB218
Lsi04G006220	Silver-seed gourd	carlsiB453
Lsi04G006220	Cucumber (Chinese Long) v3	cuclsiB131
Lsi04G006220	Cucumber (Chinese Long) v3	cuclsiB393
Lsi04G006220	Watermelon (97103) v2	lsiwmbB284
Lsi04G006220	Watermelon (97103) v2	lsiwmbB305
Lsi04G006220	Wax gourd	lsiwgoB380