Lsi04G001550 (gene) Bottle gourd (USVL1VR-Ls)

Overview
NameLsi04G001550
Typegene
OrganismLagenaria siceraria (Bottle gourd (USVL1VR-Ls))
DescriptionSubtilisin-like serine endopeptidase family protein
Locationchr04 : 1753882 .. 1759048 (+)
Genome Viewer
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: CDSthree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGATATTGTGTCTTCTTTATAGTAAAATCATTGGAGCTCGATCATATCGTAGTGACGGTAAACACCCAGAAGGCGACATCAAAGGTCCAAGAGATTCGGATGGCCACGGGACACACACTGCGTCAATTGTAGCCGGAGGATTGGTTCGGCAATCAAGCATGTTGGGACTTGCCCTTGGCACGGCAAGGGGCGGAGTCCCATCAGCACGCATTGCCGCATACAAAGTATGCTGGTCCGATGGCTGCACGGACGCCGACATTCTTGCTGCATTCGACGATGCCATCGCGGACAGTGTCGATATCATCTCCGGCTCTCTTGGGGGATCAAGGGTCAGAGATTACTTCAACGACTCCCTAGCCATTGGATCTTTTCATGCAATGAAGAAGGGAATTCTTACGTCATTGGCCGTTGGAAATAACGGCCCAGATTTCACAAGCATTGTGAACTTCTCGCCGTGGTCGTTGTCGGTGGCGGCTAGCACCACTGATCGAAAATTCGAAACTAAAGTTGAGCTCGGAGATGGAAGAGAATTCAATGTAAGAAATTTAATTAATTAATTTAATTTAAGCCCTCGTCATTTTATTAATTTTTTAAAAAAGAAAAAAATTTATTAAATTTATTAAACTAATTATATAATTTAATTTTGGTATATGAAGGGAGTCAGCATCAATACATTTGATATAAATGGAAAACAAATTCCATTGGTTTACGCCGGAGATATTCCTAAAGCTCCCTTCGACAGTTCCGTCTCCAGGTATGTCTAAATTTAGCTCTTACATATTTAATAAAATTTAAAATTAAGTTTTTAAGTTTATTTTTATCAAAATTAATTGAAGGGAAATTTTTTAAAATAGAAAAATAAGAAAAACTATTTACATAAAATAGTAAAAATTTTAGATAGTTATTGATAGACGCGGATAGAAGTCTATTAGGATCTATCAGTGATAAATTAAATTATAGAAGTCTATCAATGTATATTAGTGTTTTTTTTGTAAACATTTTGACATTTTTTCTATCTATGTAAATTTTCCTTAATTGAATGATGATAATAATTTGGTTAAAATAAAATTATTATGATCATTCAATTAACAAAACTATTATCAATTGATTGAGGTTTATTTTTATCGAAACTAAGTTTGTTAGTTCAAAATTGGACATGTCAAAGTACAATAATAGTATTTTAACCTAATAATTTTCATTTAAAAAAAAAACACAGTGTGAATATATGTTTATAAAATTTATAGAGGTAATTAAGGCTAATTAGAAATTACAAACATGTTTAATTGGGAGTTGATTTTGAAATGGTTAATAACTTTTTCATGGTCAAAATTTATCCTAAACACATACATTTAATAATTTTAAATTAATTAATTTTTGTACCATCAAAATTAATTTTGAATGATTTAAAGTGTATGATTAGAAATAATTTTAAACGTAACTAAAGTAATTTTAATCATTTCAAAACTACCCTTCCAATATGTTTTTATTCGCCCTAAAAATAATATATTTGAGAGTGATTTTAACTATTTCAAAACAACTTTCATCTGGTCGTAAAAATTTTAATAGAAAAAAATAAATAATGAACTAACAAAATGACCAATTTGGCCTTTGAAAAACAGGATGTGCTTTGAGAATACAGTGGACTTGGAGTTGGTAAAAGGTAAAATTGTAGTATGTGATTCATTGGCTGTTCCAGGAGGAGTTGTGGCTGTAAAAGGAGCGGCGGGTATTATAATGCAAGATGATTCTTCACCTGATGATACCAATTCTTTTCCATTGCCTGCTTCTCATCTTGGCCCAAAAGCTGGTGCTCTCATTCTCTCTTATATCAACTCAACCAAGAGGTAATTAATCAAATACTTTAACCTTAATTCAATTCTATTTTACACTAATTAATCATCACTCACGTGCTTTGATAATATTACATGAAAAATACTTACTCGCTAGCTGGGTTACGCTCGTTTCTTTTGCCTTAGTATATCCAATCACACTTTGTCATGTGGCCAACTTTATGCATATTTAAAAAGAAAAAAAAAATTATGATATTCTTTTCTTGTGTGTGGCGGATTGAGCTACAAAAAATTAATAAAGTCCAACTAGAGCATACAAGTATTGCTTATAGAAGAATATACAGGATGCACTCATCTTTTTGCAGTTCATCTAGTATTAGATAACATAGGCTATCCTTATAAAAATTGTGAGGTCTTTTGATCTTTTTCCTATGTGAGATCTTCGGCATGTGCTTCCTTATTACCTTCAAGATGGAACCTCTTTGGATTCACTAATCTTGGATCGGATCTCAATTTTTGTTTTTGGATTGAACACTTGCACTTATGGAGATTTGGTACCATATTAGAAAGGCGCAGTTGCAAATATAGCAATTGAGCCCAAAGTATTAGAAGATATAGTACAACCAAAAGAATTTGCAAACATAAAAAAAATTAGATCTAGCCATCGGAGTCTATCAGGTGATAGACTATATGGTTGATAGGAGTTTATCACTTATAAGAATTTATAATCAGTGATAGAGTTGATATATTTTGCTATATGAACAATTCTTTAAAAATGTTGTCGTACACTTAATTAATAGTCTTAAAATTGTTACTCATTGCAATTACTCTATTAGATAACATGGGGTTAACCCCAAAACCAATTAGCAATGAGAGAAGTAATTCATAGATTTTATGAAGGATTGTGAAATCTTTTGATTTTTCCAACGTGCATGAGATGCTCAACATCTAGTTGTCCAAATCACAATTTGTCACATGACAAACTTTTCTACTTTTTTTTCTATATTTAAAAAAATACTTTTAAATTTTCACTTCTGTGTAGTTATGATAAACTGTAAAAAGATAGGTGTAGCTCAGAATGTTGCTTTCCTATATTAGATTAAGTAAGGGATTAATTGGTTAATTACACGATGATTTACAGTCTTCCAACAGCAACTATAATGAAGAGCACAGAAAGGAAACGTAAAAGAGCTCCATTTGTTGCATCCTTTTCATCAAGAGGTCCAAACCCAATAACCCCCAACATTCTCAAGGTAATTATAATATTTATTTTATTAATTTTTTTGGAGTAATTATTGTCTAAAAATAATTAGTAATTAGTAATTTGTTGTGTGGAATATTGTTCAGCCGGATTTATGTGGTCCAGGAGTTGAAATTCTTGCAGCTTGGCCTCCAATAGCTTCGCCCTCAGGAGCTGAAGAAGATAGTAAAAGAGTTCTTTATAATATCCTTTCAGGAACTTCAATGGCTTGCCCACATGCCACTGCAGCAGCTGCTTATGTTAAATCATTTCATCCTACTTGGTCTCCTGCTGCACTTAAATCTGCCCTTATCACTACAGGTAATTAATACAAAACATTTCATATTCTAAATTAGGGTCATATAACAATTAATTTCATTTTTCTAAACTTATATATGTGAATTTTCTATGATGAAATTTTAAAAACAAAATATATATTTTTAAAATATTTAACGTTTGTTTATTTGGTAGACTATATGGAATAAGTATAATTACAATGAGTAACAATTTTAGGAATAATAACCAAGTGTATAGCAACATTTTAAAAAAATTGTAAATATAGCAAAGTCTATCACGGATAGATTTCTATCCCTGATAGACTCTTATGGTCTGCATATATAGTAAAGTCTATCAATGATAGACTTTACTATATTTGCAATTTTTTTTTAAAAATGTTACTATATATGTTAATACTTTGAACTTGATTGTTATATTTGCAACGATGTCTATGAATTGTGTTTTATCTTTTATGTTTTCTAATAAAATTCAACAAAATAAAATTGTGATCCAAATTATAGTGTTAATTTTGAAAAACAAATGAAAAGGTAGAATTACATTAAAAAAAATAAAACTTTAACAAAGGTAACATTCATGTTGTAAACTCTATTTTTTAAACATATAATATGGTATCTTATAAACTATATGATATTTTAAACTAATAATTATACAATTTTTTCATAACAAGACCAATAAAATCATGAGGTCTTTTTCATTTTGATATGTGAGGTCAATTAGACCCTTACTCTTTTTTTTAACATTTTTTTTGTTTTGATTTTTGAAAATATGAGTAGAAAATGGATAACAAAATTAAAAGAAAGTCATGGACAAAAGTAGTGTTATACGAGCTTAATTTTTTAAAACACGACCAAAAATTAAAATTTAAATAATTATCAAACAAAGTCTAATTAGTATTTTATTTTTAAAATTAACCAATCTAAATCTATATACATGCAGCATTTCCAATGAGCCCTAAACGTAATCCAGACGGAGAGTTTGGATACGGTGCCGGTCACATCAATCCACTGGGTGCAGTGCATTCTGGATTAATTTACAATGCTTCTGAGATTGACTATGTAAAGTTTCTCTGCGGTCAAGGTATTACGTTGAAAAATATGAATAAAAGTACTTTATTATTATTTTTAAGTAAAAAACACGCAACTTTAATGAAGGATATGTTTTCTTATTTTTGTGAACTTATTCGAATACATTTTTTTCATTGTTTTCTTACACTCCTTTAATTTGTTTTTCTACAGGTTATACAACCGAGTTGCTCCAACGAGTCTCGGAAGATAACAACACTTGTTCTTCAAACAATTCCAACACAGTATTCGACCTTAACTATCCTTCTTTTGCTTTTTCCACAAATATTTCGAAACCAATCAACCACGTTTACAGAAGGAGCGTGACCAATGTTGGATCAAAATATGCAACATATAAAGCCACCATAATTAATCCATGGAAGAATCTTGAGATCAAAGTAAAGCCTTCTATTCTTTCATTCAAGAATTTGGGAGAGGAGCAAAGCTTTGAGCTTACAATTAAAGGAAGAATTAGTAAAAATATTGAATCAGCTTCCTTGGTATGGGATGATGGGAAACACAAAGTGAGGAGTCCTATAACAGTGTTTGATGCTAATATTCACTCTTCTTAATTAAATTTCCAACTTATTAATTAAATAATATTTCTAAAAATATGTAATTCATGTATTTTTCTTAAATTGTAGGGTCAAATAGAGTGGTTTTGTTGGTTTCGCAGCTTAATTTATTGGTCAAAAAGACATATTTGACCTCATGATGTGTTATGAATTCCCTCTATTGAATATACATACGATCAATAAAGCATATTTGTCTTCCTTCC

mRNA sequence

ATGATATTTAAAATCATTGGAGCTCGATCATATCGTAGTGACGGTAAACACCCAGAAGGCGACATCAAAGGTCCAAGAGATTCGGATGGCCACGGGACACACACTGCGTCAATTGTAGCCGGAGGATTGGTTCGGCAATCAAGCATGTTGGGACTTGCCCTTGGCACGGCAAGGGGCGGAGTCCCATCAGCACGCATTGCCGCATACAAAGTATGCTGGTCCGATGGCTGCACGGACGCCGACATTCTTGCTGCATTCGACGATGCCATCGCGGACAGTGTCGATATCATCTCCGGCTCTCTTGGGGGATCAAGGGTCAGAGATTACTTCAACGACTCCCTAGCCATTGGATCTTTTCATGCAATGAAGAAGGGAATTCTTACGTCATTGGCCGTTGGAAATAACGGCCCAGATTTCACAAGCATTGTGAACTTCTCGCCGTGGTCGTTGTCGGTGGCGGCTAGCACCACTGATCGAAAATTCGAAACTAAAGTTGAGCTCGGAGATGGAAGAGAATTCAATGGAGTCAGCATCAATACATTTGATATAAATGGAAAACAAATTCCATTGGTTTACGCCGGAGATATTCCTAAAGCTCCCTTCGACAGTTCCGTCTCCAGGATGTGCTTTGAGAATACAGTGGACTTGGAGTTGGTAAAAGGTAAAATTGTAGTATGTGATTCATTGGCTGTTCCAGGAGGAGTTGTGGCTGTAAAAGGAGCGGCGGGTATTATAATGCAAGATGATTCTTCACCTGATGATACCAATTCTTTTCCATTGCCTGCTTCTCATCTTGGCCCAAAAGCTGGTGCTCTCATTCTCTCTTATATCAACTCAACCAAGAGAGGTCCAAACCCAATAACCCCCAACATTCTCAAGCCGGATTTATGTGGTCCAGGAGTTGAAATTCTTGCAGCTTGGCCTCCAATAGCTTCGCCCTCAGGAGCTGAAGAAGATAGAACTTCAATGGCTTGCCCACATGCCACTGCAGCAGCTGCTTATGTTAAATCATTTCATCCTACTTGGTCTCCTGCTGCACTTAAATCTGCCCTTATCACTACAGGTTATACAACCGAGTTGCTCCAACGAGTCTCGGAAGATAACAACACTTGTTCTTCAAACAATTCCAACACAGTATTCGACCTTAACTATCCTTCTTTTGCTTTTTCCACAAATATTTCGAAACCAATCAACCACGTTTACAGAAGGAGCGTGACCAATGTTGGATCAAAATATGCAACATATAAAGCCACCATAATTAATCCATGGAAGAATCTTGAGATCAAAGTAAAGCCTTCTATTCTTTCATTCAAGAATTTGGGAGAGGAGCAAAGCTTTGAGCTTACAATTAAAGGAAGAATTAGTAAAAATATTGAATCAGCTTCCTTGGTATGGGATGATGGGAAACACAAAGTGAGGAGTCCTATAACAGTGTTTGATGCTAATATTCACTCTTCTTAATTAAATTTCCAACTTATTAATTAAATAATATTTCTAAAAATATGTAATTCATGTATTTTTCTTAAATTGTAGGGTCAAATAGAGTGGTTTTGTTGGTTTCGCAGCTTAATTTATTGGTCAAAAAGACATATTTGACCTCATGATGTGTTATGAATTCCCTCTATTGAATATACATACGATCAATAAAGCATATTTGTCTTCCTTCC

Coding sequence (CDS)

ATGATATTTAAAATCATTGGAGCTCGATCATATCGTAGTGACGGTAAACACCCAGAAGGCGACATCAAAGGTCCAAGAGATTCGGATGGCCACGGGACACACACTGCGTCAATTGTAGCCGGAGGATTGGTTCGGCAATCAAGCATGTTGGGACTTGCCCTTGGCACGGCAAGGGGCGGAGTCCCATCAGCACGCATTGCCGCATACAAAGTATGCTGGTCCGATGGCTGCACGGACGCCGACATTCTTGCTGCATTCGACGATGCCATCGCGGACAGTGTCGATATCATCTCCGGCTCTCTTGGGGGATCAAGGGTCAGAGATTACTTCAACGACTCCCTAGCCATTGGATCTTTTCATGCAATGAAGAAGGGAATTCTTACGTCATTGGCCGTTGGAAATAACGGCCCAGATTTCACAAGCATTGTGAACTTCTCGCCGTGGTCGTTGTCGGTGGCGGCTAGCACCACTGATCGAAAATTCGAAACTAAAGTTGAGCTCGGAGATGGAAGAGAATTCAATGGAGTCAGCATCAATACATTTGATATAAATGGAAAACAAATTCCATTGGTTTACGCCGGAGATATTCCTAAAGCTCCCTTCGACAGTTCCGTCTCCAGGATGTGCTTTGAGAATACAGTGGACTTGGAGTTGGTAAAAGGTAAAATTGTAGTATGTGATTCATTGGCTGTTCCAGGAGGAGTTGTGGCTGTAAAAGGAGCGGCGGGTATTATAATGCAAGATGATTCTTCACCTGATGATACCAATTCTTTTCCATTGCCTGCTTCTCATCTTGGCCCAAAAGCTGGTGCTCTCATTCTCTCTTATATCAACTCAACCAAGAGAGGTCCAAACCCAATAACCCCCAACATTCTCAAGCCGGATTTATGTGGTCCAGGAGTTGAAATTCTTGCAGCTTGGCCTCCAATAGCTTCGCCCTCAGGAGCTGAAGAAGATAGAACTTCAATGGCTTGCCCACATGCCACTGCAGCAGCTGCTTATGTTAAATCATTTCATCCTACTTGGTCTCCTGCTGCACTTAAATCTGCCCTTATCACTACAGGTTATACAACCGAGTTGCTCCAACGAGTCTCGGAAGATAACAACACTTGTTCTTCAAACAATTCCAACACAGTATTCGACCTTAACTATCCTTCTTTTGCTTTTTCCACAAATATTTCGAAACCAATCAACCACGTTTACAGAAGGAGCGTGACCAATGTTGGATCAAAATATGCAACATATAAAGCCACCATAATTAATCCATGGAAGAATCTTGAGATCAAAGTAAAGCCTTCTATTCTTTCATTCAAGAATTTGGGAGAGGAGCAAAGCTTTGAGCTTACAATTAAAGGAAGAATTAGTAAAAATATTGAATCAGCTTCCTTGGTATGGGATGATGGGAAACACAAAGTGAGGAGTCCTATAACAGTGTTTGATGCTAATATTCACTCTTCTTAA

Protein sequence

MIFKIIGARSYRSDGKHPEGDIKGPRDSDGHGTHTASIVAGGLVRQSSMLGLALGTARGGVPSARIAAYKVCWSDGCTDADILAAFDDAIADSVDIISGSLGGSRVRDYFNDSLAIGSFHAMKKGILTSLAVGNNGPDFTSIVNFSPWSLSVAASTTDRKFETKVELGDGREFNGVSINTFDINGKQIPLVYAGDIPKAPFDSSVSRMCFENTVDLELVKGKIVVCDSLAVPGGVVAVKGAAGIIMQDDSSPDDTNSFPLPASHLGPKAGALILSYINSTKRGPNPITPNILKPDLCGPGVEILAAWPPIASPSGAEEDRTSMACPHATAAAAYVKSFHPTWSPAALKSALITTGYTTELLQRVSEDNNTCSSNNSNTVFDLNYPSFAFSTNISKPINHVYRRSVTNVGSKYATYKATIINPWKNLEIKVKPSILSFKNLGEEQSFELTIKGRISKNIESASLVWDDGKHKVRSPITVFDANIHSS
Homology
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BLAST of Lsi04G001550 vs. Swiss-Prot
Match: CUCM1_CUCME (Cucumisin OS=Cucumis melo PE=1 SV=1)

HSP 1 Score: 375.2 bits (962), Expect = 1.1e-102
Identity = 204/384 (53.12%), Postives = 258/384 (67.19%), Query Frame = 1 

Query: 4   KIIGARSYRSDGKHPEGDIKGPRDSDGHGTHTASIVAGGLVRQSSMLGLALGTARGGVPS 63
           KIIGARSY        GD+ GPRD++GHGTHTAS  AGGLV Q+++ GL LGTARGGVP 
Sbjct: 177 KIIGARSYHIGRPISPGDVNGPRDTNGHGTHTASTAAGGLVSQANLYGLGLGTARGGVPL 236

Query: 64  ARIAAYKVCWSDGCTDADILAAFDDAIADSVDIISGSLGGSRVRDYFNDSLAIGSFHAMK 123
           ARIAAYKVCW+DGC+D DILAA+DDAIAD VDIIS S+GG+  R YF D++AIGSFHA++
Sbjct: 237 ARIAAYKVCWNDGCSDTDILAAYDDAIADGVDIISLSVGGANPRHYFVDAIAIGSFHAVE 296

Query: 124 KGILTSLAVGNNGPDFTSIVNFSPWSLSVAASTTDRKFETKVELGDGREFNGVSINTFDI 183
           +GILTS + GN GP+F +  + SPW LSVAAST DRKF T+V++G+G+ F GVSINTFD 
Sbjct: 297 RGILTSNSAGNGGPNFFTTASLSPWLLSVAASTMDRKFVTQVQIGNGQSFQGVSINTFD- 356

Query: 184 NGKQIPLVYAGDIPKAPFDSSVSRMCFENTVDLELVKGKIVVCDSLAVPGGVV-AVKGAA 243
             +  PLV   DIP   FD S SR C + +V+  L+KGKIVVC++   P     ++ GAA
Sbjct: 357 -NQYYPLVSGRDIPNTGFDKSTSRFCTDKSVNPNLLKGKIVVCEASFGPHEFFKSLDGAA 416

Query: 244 GIIMQDDSSPDDTNSFPLPASHLGPKAGALILSYINS----------------------- 303
           G++M  ++  D  +S+PLP+S L P      L YI S                       
Sbjct: 417 GVLMTSNTR-DYADSYPLPSSVLDPNDLLATLRYIYSIRSPGATIFKSTTILNASAPVVV 476

Query: 304 --TKRGPNPITPNILKPDLCGPGVEILAAWPPIASPSGAEEDR-------TSMACPHATA 355
             + RGPN  T +++KPD+ GPGVEILAAWP +A   G   +        TSM+CPH T 
Sbjct: 477 SFSSRGPNRATKDVIKPDISGPGVEILAAWPSVAPVGGIRRNTLFNIISGTSMSCPHITG 536

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BLAST of Lsi04G001550 vs. Swiss-Prot
Match: SBT43_ARATH (Subtilisin-like protease SBT4.3 OS=Arabidopsis thaliana GN=SBT4.3 PE=3 SV=1)

HSP 1 Score: 360.9 bits (925), Expect = 2.2e-98
Identity = 228/560 (40.71%), Postives = 309/560 (55.18%), Query Frame = 1 

Query: 4   KIIGARSYRSDGKHPEGDIKGPRDSDGHGTHTASIVAGGLVRQSSMLGLALGTARGGVPS 63
           K+IGAR Y              RD +GHGTHTAS  AG  V+ +S  GLA GTARGGVPS
Sbjct: 176 KLIGARFYNKFAD-------SARDEEGHGTHTASTAAGNAVQAASFYGLAQGTARGGVPS 235

Query: 64  ARIAAYKVCWSDGCTDADILAAFDDAIADSVDIISGSLGGSRVRDYFNDSLAIGSFHAMK 123
           ARIAAYKVC++  C D DILAAFDDAIAD VD+IS S+    V +  N S+AIGSFHAM 
Sbjct: 236 ARIAAYKVCFNR-CNDVDILAAFDDAIADGVDVISISISADYVSNLLNASVAIGSFHAMM 295

Query: 124 KGILTSLAVGNNGPDFTSIVNFSPWSLSVAASTTDRKFETKVELGDGREFNGVSINTFDI 183
           +GI+T+ + GNNGPD  S+ N SPW ++VAAS TDR+F  +V LG+G+   G+S+NTF++
Sbjct: 296 RGIITAGSAGNNGPDQGSVANVSPWMITVAASGTDRQFIDRVVLGNGKALTGISVNTFNL 355

Query: 184 NGKQIPLVYAGDIPKAPFDSSVSRMCFENTVDLELVKGKIVVCDSLAVPGGVVAVKGAAG 243
           NG + P+VY  ++ +    +     C    VD ELVKGKIV+CD   +      + GA G
Sbjct: 356 NGTKFPIVYGQNVSRNCSQAQAG-YCSSGCVDSELVKGKIVLCDDF-LGYREAYLAGAIG 415

Query: 244 IIMQDDSSPDDTNSFPLPASHLGPKAGALILSYINSTK---------------------- 303
           +I+Q+   PD     P PAS LG +    I SYI S +                      
Sbjct: 416 VIVQNTLLPDSAFVVPFPASSLGFEDYKSIKSYIESAEPPQAEILRTEEIVDREAPYVPS 475

Query: 304 ---RGPNPITPNILKPDLCGPGVEILAAWPPIASPS---GAEEDR---------TSMACP 363
              RGP+ +  N+LKPD+  PG+EILAA+ P+ASPS     E+ R         TSMACP
Sbjct: 476 FSSRGPSFVIQNLLKPDVSAPGLEILAAFSPVASPSSFLNPEDKRSVRYSVMSGTSMACP 535

Query: 364 HATAAAAYVKSFHPTWSPAALKSALITTGYTTELLQRVSED-------NNTCSSNNSNTV 423
           H    AAYVKSFHP WSP+A+KSA++TT     L +   ++        N   +++   V
Sbjct: 536 HVAGVAAYVKSFHPDWSPSAIKSAIMTTATPMNLKKNPEQEFAYGSGQINPTKASDPGLV 595

Query: 424 FDLNYPSFA------------------------------------FSTNISK--PINHVY 480
           +++    +                                      +T +S   P N  +
Sbjct: 596 YEVETEDYLKMLCAEGFDSTTLTTTSGQNVTCSERTEVKDLNYPTMTTFVSSLDPFNVTF 655

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BLAST of Lsi04G001550 vs. Swiss-Prot
Match: SBT4E_ARATH (Subtilisin-like protease SBT4.14 OS=Arabidopsis thaliana GN=SBT4.14 PE=2 SV=1)

HSP 1 Score: 319.7 bits (818), Expect = 5.5e-86
Identity = 181/402 (45.02%), Postives = 247/402 (61.44%), Query Frame = 1 

Query: 4   KIIGARSYRSDGKHPEGDIKGPRDSDGHGTHTASIVAGGLVRQSSMLGLALGTARGGVPS 63
           KIIGA+ ++ DG  P G+++ P D DGHGTHT+S VAG LV  +S+ G+A GTARG VPS
Sbjct: 183 KIIGAKYFKHDGNVPAGEVRSPIDIDGHGTHTSSTVAGVLVANASLYGIANGTARGAVPS 242

Query: 64  ARIAAYKVCWS-DGCTDADILAAFDDAIADSVDIISGSLGGSRVRDYFNDSLAIGSFHAM 123
           AR+A YKVCW+  GC D DILA F+ AI D V+IIS S+GG  + DY +DS+++GSFHAM
Sbjct: 243 ARLAMYKVCWARSGCADMDILAGFEAAIHDGVEIISISIGGP-IADYSSDSISVGSFHAM 302

Query: 124 KKGILTSLAVGNNGPDFTSIVNFSPWSLSVAASTTDRKFETKVELGDGREFNGVSINTFD 183
           +KGILT  + GN+GP   ++ N  PW L+VAAS  DR F++K++LG+G+ F+G+ I+ F 
Sbjct: 303 RKGILTVASAGNDGPSSGTVTNHEPWILTVAASGIDRTFKSKIDLGNGKSFSGMGISMFS 362

Query: 184 INGKQIPLVYAGDIPKAPFDSSVSRMCFENTVDLELVKGKIVVCDSLAVPGGV---VAVK 243
              K  PLV   D  K   D  ++R CF +++D + VKGK++VC      GGV   +   
Sbjct: 363 PKAKSYPLVSGVDAAKNTDDKYLARYCFSDSLDRKKVKGKVMVCRMGG--GGVESTIKSY 422

Query: 244 GAAGIIMQDDSSPDDTNSFPLPASHLGPKAGALILSYINSTK------------------ 303
           G AG I+  D   D+   F  PA+ +    G +I  YINST+                  
Sbjct: 423 GGAGAIIVSDQYLDNAQIFMAPATSVNSSVGDIIYRYINSTRSASAVIQKTRQVTIPAPF 482

Query: 304 ------RGPNPITPNILKPDLCGPGVEILAAWPPIASPSGAEEDR----------TSMAC 363
                 RGPNP +  +LKPD+  PG++ILAA+    S +G + D           TSMAC
Sbjct: 483 VASFSSRGPNPGSIRLLKPDIAAPGIDILAAFTLKRSLTGLDGDTQFSKFTILSGTSMAC 542

Query: 364 PHATAAAAYVKSFHPTWSPAALKSALITTGYTTELLQRVSED 368
           PH    AAYVKSFHP W+PAA+KSA+IT+     + +RV++D
Sbjct: 543 PHVAGVAAYVKSFHPDWTPAAIKSAIITS--AKPISRRVNKD 579

[Close]
BLAST of Lsi04G001550 vs. Swiss-Prot
Match: SBT4F_ARATH (Subtilisin-like protease SBT4.15 OS=Arabidopsis thaliana GN=SBT4.15 PE=3 SV=1)

HSP 1 Score: 305.8 bits (782), Expect = 8.2e-82
Identity = 183/398 (45.98%), Postives = 244/398 (61.31%), Query Frame = 1 

Query: 4   KIIGARSY--RSDGKHPEGDIKGPRDSDGHGTHTASIVAGGLVRQSSMLGLALGTARGGV 63
           K+IGA+ +  +S+G  P+G+     D DGHGTHT+S +AG  V  +S+ G+A GTARGGV
Sbjct: 182 KVIGAKYFHIQSEGL-PDGEGDTAADHDGHGTHTSSTIAGVSVSSASLFGIANGTARGGV 241

Query: 64  PSARIAAYKVCWSDGCTDADILAAFDDAIADSVDIISGSLGGSRVRDYFNDSLAIGSFHA 123
           PSARIAAYKVCW  GCTD D+LAAFD+AI+D VDIIS S+GG+ +  +F D +AIG+FHA
Sbjct: 242 PSARIAAYKVCWDSGCTDMDMLAAFDEAISDGVDIISISIGGASL-PFFEDPIAIGAFHA 301

Query: 124 MKKGILTSLAVGNNGPDFTSIVNFSPWSLSVAASTTDRKFETKVELGDGREFNGVSINTF 183
           MK+GILT+ + GNNGP   ++ N +PW ++VAA++ DRKFET V+LG+G   +G+S+N F
Sbjct: 302 MKRGILTTCSAGNNGPGLFTVSNLAPWVMTVAANSLDRKFETVVKLGNGLTASGISLNGF 361

Query: 184 DINGKQIPLV---YAGDIPKAPFDSSVSRMCFENTVDLELVKGKIVVCDSLAVPGG---- 243
           +   K  PL     A ++    +    +  C   T+  + V GK+V C++    GG    
Sbjct: 362 NPRKKMYPLTSGSLASNLSAGGYGEPST--CEPGTLGEDKVMGKVVYCEAGREEGGNGGQ 421

Query: 244 ----VVAVKGAAGIIMQDDSSPDDTNSFPLPASHLGPKAGALILSYINSTK--------- 303
               VV     AG+I+Q     D   S  +  S++  + G  I  YINSTK         
Sbjct: 422 GQDHVVRSLKGAGVIVQLLEPTDMATSTLIAGSYVFFEDGTKITEYINSTKNPQAVIFKT 481

Query: 304 ---------------RGPNPITPNILKPDLCGPGVEILAAWPPIASPSGAEEDR------ 355
                          RGP  I+PNILKPD+  PG+ ILAA+  +AS +G  +D       
Sbjct: 482 KTTKMLAPSISSFSARGPQRISPNILKPDISAPGLNILAAYSKLASVTGYPDDNRRTLFS 541

[Close]
BLAST of Lsi04G001550 vs. Swiss-Prot
Match: SBT17_ARATH (Subtilisin-like protease SBT1.7 OS=Arabidopsis thaliana GN=SBT1.7 PE=1 SV=1)

HSP 1 Score: 301.2 bits (770), Expect = 2.0e-80
Identity = 213/584 (36.47%), Postives = 296/584 (50.68%), Query Frame = 1 

Query: 4   KIIGARSYRSDGKHPEGDI------KGPRDSDGHGTHTASIVAGGLVRQSSMLGLALGTA 63
           K+IGAR +    +   G I      + PRD DGHGTHT+S  AG +V  +S+LG A GTA
Sbjct: 179 KLIGARFFARGYESTMGPIDESKESRSPRDDDGHGTHTSSTAAGSVVEGASLLGYASGTA 238

Query: 64  RGGVPSARIAAYKVCWSDGCTDADILAAFDDAIADSVDIISGSLGGSRVRDYFNDSLAIG 123
           RG  P AR+A YKVCW  GC  +DILAA D AIAD+V+++S SLGG  + DY+ D +AIG
Sbjct: 239 RGMAPRARVAVYKVCWLGGCFSSDILAAIDKAIADNVNVLSMSLGGG-MSDYYRDGVAIG 298

Query: 124 SFHAMKKGILTSLAVGNNGPDFTSIVNFSPWSLSVAASTTDRKFETKVELGDGREFNGVS 183
           +F AM++GIL S + GN GP  +S+ N +PW  +V A T DR F     LG+G+ F GVS
Sbjct: 299 AFAAMERGILVSCSAGNAGPSSSSLSNVAPWITTVGAGTLDRDFPALAILGNGKNFTGVS 358

Query: 184 INTFD-INGKQIPLVYAGDIPKAPFDSSVSRMCFENTVDLELVKGKIVVCD----SLAVP 243
           +   + +  K +P +YAG+   A    +   +C   T+  E VKGKIV+CD    +    
Sbjct: 359 LFKGEALPDKLLPFIYAGNASNA----TNGNLCMTGTLIPEKVKGKIVMCDRGINARVQK 418

Query: 244 GGVVAVKGAAGIIMQDDSSPDD---TNSFPLPASHLGPKAGALILSYINSTKRGPNP--- 303
           G VV   G  G+I+ + ++  +    ++  LPA+ +G KAG +I  Y+ +    PNP   
Sbjct: 419 GDVVKAAGGVGMILANTAANGEELVADAHLLPATTVGEKAGDIIRHYVTT---DPNPTAS 478

Query: 304 --------------------------ITPNILKPDLCGPGVEILAAWPPIASPSGAEEDR 363
                                     ITPNILKPDL  PGV ILAAW   A P+G   D 
Sbjct: 479 ISILGTVVGVKPSPVVAAFSSRGPNSITPNILKPDLIAPGVNILAAWTGAAGPTGLASDS 538

Query: 364 ----------TSMACPHATAAAAYVKSFHPTWSPAALKSALITTGYTT-----ELLQRVS 423
                     TSM+CPH +  AA +KS HP WSPAA++SAL+TT Y T      LL   +
Sbjct: 539 RRVEFNIISGTSMSCPHVSGLAALLKSVHPEWSPAAIRSALMTTAYKTYKDGKPLLDIAT 598

Query: 424 EDNNT-----------CSSNNSNTVFDL---NYPSFAFSTNISKP-INHVYRRSVTNVGS 479
              +T            ++ N   ++DL   +Y  F  + N + P I  V RR+ T   S
Sbjct: 599 GKPSTPFDHGAGHVSPTTATNPGLIYDLTTEDYLGFLCALNYTSPQIRSVSRRNYTCDPS 658

[Close]
BLAST of Lsi04G001550 vs. TrEMBL
Match: A0A0A0KWL9_CUCSA (Uncharacterized protein OS=Cucumis sativus GN=Csa_5G613590 PE=4 SV=1)

HSP 1 Score: 730.3 bits (1884), Expect = 1.5e-207
Identity = 395/567 (69.66%), Postives = 435/567 (76.72%), Query Frame = 1 

Query: 4   KIIGARSYRSDGKHPEGDIKGPRDSDGHGTHTASIVAGGLVRQSSMLGLALGTARGGVPS 63
           KIIGARSYRS+G++PEGDIKGPRDSDGHGTHTASIVAGGLVR++SMLGL LGTARGGVPS
Sbjct: 177 KIIGARSYRSNGEYPEGDIKGPRDSDGHGTHTASIVAGGLVRRASMLGLGLGTARGGVPS 236

Query: 64  ARIAAYKVCWSDGCTDADILAAFDDAIADSVDIISGSLGGSRVRDYFNDSLAIGSFHAMK 123
           ARIAAYKVCWSDGC+DADILAAFDDAIAD VDIISGSLGGS  RDYFNDS+AIGSFHAMK
Sbjct: 237 ARIAAYKVCWSDGCSDADILAAFDDAIADGVDIISGSLGGSGARDYFNDSIAIGSFHAMK 296

Query: 124 KGILTSLAVGNNGPDFTSIVNFSPWSLSVAASTTDRKFETKVELGDGREFNGVSINTFDI 183
           KGILTSLAVGNNGPDFT+IVNFSPWSLSVAASTTDRKFETKVELGDGREF+GVS+NTFDI
Sbjct: 297 KGILTSLAVGNNGPDFTTIVNFSPWSLSVAASTTDRKFETKVELGDGREFSGVSVNTFDI 356

Query: 184 NGKQIPLVYAGDIPKAPFDSSVSRMCFENTVDLELVKGKIVVCDSLAVPGGVVAVKGAAG 243
            GKQIPLVYAGDIPKAPFDSSVSR+CFENTVDL+LVKGKIVVCDSL VPGGVVAVKGA G
Sbjct: 357 KGKQIPLVYAGDIPKAPFDSSVSRLCFENTVDLKLVKGKIVVCDSLTVPGGVVAVKGAVG 416

Query: 244 IIMQDDSSPDDTNSFPLPASHLGPKAGALILSYINST----------------------- 303
           IIMQDDSS DDTNSFP+PASHLGPKAGAL+LSYINST                       
Sbjct: 417 IIMQDDSSHDDTNSFPIPASHLGPKAGALVLSYINSTNSIPTATIKKSTERKRKRAPSVA 476

Query: 304 ---KRGPNPITPNILKPDLCGPGVEILAAWPPIASPSGAEEDR----------TSMACPH 363
               RGPNPITPNILKPDL GPGVEILAAW P++ PSGAEED           TSMACPH
Sbjct: 477 SFSSRGPNPITPNILKPDLSGPGVEILAAWSPVSPPSGAEEDNKRVLYNIISGTSMACPH 536

Query: 364 ATAAAAYVKSFHPTWSPAALKSALITTGYTTELLQRVSED-------NNTCSSNNSNTVF 423
            TAAAAYVKSFHPTWSP+ALKSALITT +         ++        N   + +   ++
Sbjct: 537 VTAAAAYVKSFHPTWSPSALKSALITTAFPMSPKHNPDKEFGYGAGHINPLGAVHPGLIY 596

Query: 424 D---LNYPSF----AFSTNISK------------------PINHVYRRSVTNVG------ 483
           D   ++Y  F     ++T + +                   +N+      TN+       
Sbjct: 597 DASEIDYVQFLCGQGYTTELLQLVSEDNNTCSSNNSDTVFDLNYPSFALSTNISKPINQV 656

Query: 484 ---------SKYATYKATIINPWKNLEIKVKPSILSFKNLGEEQSFELTIKGRISKNIES 487
                    SKYATYKAT+INPWKNLEIKV PS+LSFKNLGE+QSFE+TI+G+I K+IES
Sbjct: 657 YKRTVTNVGSKYATYKATVINPWKNLEIKVNPSVLSFKNLGEKQSFEVTIRGKIRKDIES 716

[Close]
BLAST of Lsi04G001550 vs. TrEMBL
Match: A0A0A0KJN5_CUCSA (Uncharacterized protein OS=Cucumis sativus GN=Csa_5G141040 PE=4 SV=1)

HSP 1 Score: 544.7 bits (1402), Expect = 1.2e-151
Identity = 309/564 (54.79%), Postives = 372/564 (65.96%), Query Frame = 1 

Query: 4   KIIGARSYRSDGKHPEGDIKGPRDSDGHGTHTASIVAGGLVRQSSMLGLALGTARGGVPS 63
           KIIGARSYRSDG++P  DIKGPRDS+GHGTH AS VAGGLVRQ+SMLGL +GTARGGVPS
Sbjct: 177 KIIGARSYRSDGRYPIDDIKGPRDSNGHGTHAASTVAGGLVRQASMLGLGMGTARGGVPS 236

Query: 64  ARIAAYKVCWSDGCTDADILAAFDDAIADSVDIISGSLGGSRVR-DYFNDSLAIGSFHAM 123
           ARIAAYKVCWSD C+DAD+LAAFDDAIAD VDIIS S+G  R R +YF D +AIG+FHAM
Sbjct: 237 ARIAAYKVCWSDTCSDADVLAAFDDAIADGVDIISMSVGPKRPRPNYFQDPIAIGTFHAM 296

Query: 124 KKGILTSLAVGNNGPDFTSIVNFSPWSLSVAASTTDRKFETKVELGDGREFNGVSINTFD 183
           + GILTS + GN GP   ++ NFSPW+LSVAAST+DR+F T V+LGDGR+FNGV+INTFD
Sbjct: 297 RNGILTSTSAGNEGPLHFTVTNFSPWALSVAASTSDRRFLTAVQLGDGRKFNGVTINTFD 356

Query: 184 INGKQIPLVYAGDIPKAP--FDSSVSRMCFENTVDLELVKGKIVVCDSLAVPGGVVAVKG 243
           +NG Q PLVYAG+IP     F+ S SR C  ++VD ELVKGKI +CDS   P  V +++ 
Sbjct: 357 LNGTQYPLVYAGNIPNVTGGFNGSFSRFCLRDSVDRELVKGKIAICDSFVSPSDVGSLES 416

Query: 244 AAGIIMQDDSSPDDTNSFPLPASHLGPKAGALILSYINSTK------------------- 303
           A GIIMQD S  D T +FPLPASHLG +   LI SY+NST+                   
Sbjct: 417 AVGIIMQDRSPKDLTFAFPLPASHLGIQQRPLISSYLNSTRIPTATILKSTGLKLQVAPL 476

Query: 304 ------RGPNPITPNILKPDLCGPGVEILAAWPPIASPSGAEEDR----------TSMAC 363
                 RGPNP +P ILKPD+ GPGVEILAAW P+ SPS A+ D           TSMAC
Sbjct: 477 VASFSSRGPNPTSPYILKPDVIGPGVEILAAWSPLRSPSNAKGDNRKLLFNIISGTSMAC 536

Query: 364 PHATAA-----------------AAYVKSFHP----------------------TWSPAA 423
           PHATA                  +A + +  P                        +P  
Sbjct: 537 PHATAVAAYVKSFHPSWSPAALKSALITTAFPMRGDLYPEAEFAYGSGHINPLGAVNPGL 596

Query: 424 LKSA--------LITTGYTTELLQRVSEDNNTCSSNNSNTVFDLNYPSFAFSTNISKPIN 483
           + +A        L   GY T  L+ +++DN+TCS+  S  V+DLNYPSFA  T+IS P +
Sbjct: 597 IYNASETDYIRFLCDEGYNTTFLRIITKDNSTCSTTQSIRVYDLNYPSFALFTHISTPFS 656

[Close]
BLAST of Lsi04G001550 vs. TrEMBL
Match: A0A0A0KRC4_CUCSA (Uncharacterized protein OS=Cucumis sativus GN=Csa_5G613580 PE=4 SV=1)

HSP 1 Score: 513.5 bits (1321), Expect = 2.9e-142
Identity = 266/390 (68.21%), Postives = 296/390 (75.90%), Query Frame = 1 

Query: 4   KIIGARSYRSDGKHPEGDIKGPRDSDGHGTHTASIVAGGLVRQSSMLGLALGTARGGVPS 63
           KIIGARSY S G HPEGD++GP DS+GHGTHTAS VAGGLVRQ++MLGL LGTARGGVPS
Sbjct: 176 KIIGARSYHSSGPHPEGDLEGPIDSNGHGTHTASTVAGGLVRQANMLGLGLGTARGGVPS 235

Query: 64  ARIAAYKVCWSDGCTDADILAAFDDAIADSVDIISGSLGGSRVRDYFNDSLAIGSFHAMK 123
           ARIA YK+CWSD C+DADILAAFDDAIAD VDI+S S+ G   ++YFNDS+AIGSFHAMK
Sbjct: 236 ARIAVYKICWSDNCSDADILAAFDDAIADGVDILSVSVAGPGFKNYFNDSMAIGSFHAMK 295

Query: 124 KGILTSLAVGNNGPDFTSIVNFSPWSLSVAASTTDRKFETKVELGDGREFNGVSINTFDI 183
           KGIL+S A GN GP   S+ N+SPWSL+VAASTTDR  ET VELGDGRE  GV+INTFD+
Sbjct: 296 KGILSSFAAGNTGPGSASVANYSPWSLTVAASTTDRVLETVVELGDGRELKGVTINTFDM 355

Query: 184 NGKQIPLVYAGDIPKAPFDSSVSRMCFENTVDLELVKGKIVVCDSLAV-PGGVVAVKGAA 243
            GKQ+PLVY GDIPKA   SS S  C  N+VDL+L KGKIV+CD +   P   VAVKGA 
Sbjct: 356 KGKQVPLVYGGDIPKANTSSSFSSQCLRNSVDLKLAKGKIVMCDMITTSPAEAVAVKGAV 415

Query: 244 GIIMQDDSSPDDTNSFPLPASHLGPKAGALILSYINST---------------------- 303
           GIIMQ+DS  D T SFP+PASH+  K+GALILSYINST                      
Sbjct: 416 GIIMQNDSPKDRTFSFPIPASHIDTKSGALILSYINSTNSIPTATIKKSIERKRRRAPSV 475

Query: 304 ----KRGPNPITPNILKPDLCGPGVEILAAWPPIASPSGAEEDR----------TSMACP 357
                RGPNP+TPNILKPDL GPGVEILAAWPPIASPSGA ED           TSMACP
Sbjct: 476 ASFSSRGPNPVTPNILKPDLSGPGVEILAAWPPIASPSGAVEDNKRVLYNIISGTSMACP 535

[Close]
BLAST of Lsi04G001550 vs. TrEMBL
Match: D7TLL6_VITVI (Putative uncharacterized protein OS=Vitis vinifera GN=VIT_13s0019g02480 PE=4 SV=1)

HSP 1 Score: 483.8 bits (1244), Expect = 2.5e-133
Identity = 282/559 (50.45%), Postives = 344/559 (61.54%), Query Frame = 1 

Query: 4   KIIGARSYRSDGKHPEGDIKGPRDSDGHGTHTASIVAGGLVRQSSMLGLALGTARGGVPS 63
           KIIGAR YRS G+    D++ PRDS+GHGTHTAS  AGGLV  +S+LG  LGTARGGVPS
Sbjct: 139 KIIGARYYRSYGEFSPEDLQTPRDSEGHGTHTASTAAGGLVSMASLLGFGLGTARGGVPS 198

Query: 64  ARIAAYKVCWSDGCTDADILAAFDDAIADSVDIISGSLGGSRVRDYFNDSLAIGSFHAMK 123
           ARIA YK+CWSDGC DADILAAFDDAIAD VDIIS S+GGS  ++YF DS+AIG+FHAMK
Sbjct: 199 ARIAVYKICWSDGCADADILAAFDDAIADGVDIISLSVGGSTPKNYFADSIAIGAFHAMK 258

Query: 124 KGILTSLAVGNNGPDFTSIVNFSPWSLSVAASTTDRKFETKVELGDGREFNGVSINTFDI 183
            GILTS + GN+GP+F SI NFSPWSLSVAAST DRKF TKV+LGD + + G+SINTF+ 
Sbjct: 259 NGILTSTSAGNDGPNFASITNFSPWSLSVAASTIDRKFFTKVQLGDSKVYEGISINTFEP 318

Query: 184 NGKQIPLVYAGDIPKAP--FDSSVSRMCFENTVDLELVKGKIVVCDSLAVPGGVVAVKGA 243
           NG   P +Y GD P     F ++ SR C  N++D  LVKGKIV+CD  +   G   + GA
Sbjct: 319 NG-MYPFIYGGDAPNITGGFSANTSRFCTRNSLDPNLVKGKIVLCDIFSNGTGAF-LAGA 378

Query: 244 AGIIMQDDSSPDDTNSFPLPASHLGPKAGALILSYINSTK-------------------- 303
            G +M D  + D    FPLPAS+LG + G+ I  Y+ ST                     
Sbjct: 379 VGTVMADRGAKDSAWPFPLPASYLGAQDGSSIAYYVTSTSNPTASILKSTEVNDTLAPFI 438

Query: 304 -----RGPNPITPNILKPDLCGPGVEILAAWPPIASPSGAEEDR----------TSMACP 363
                RGPNP T +ILKPDL  PGV ILAAWPPI+  SG + D           TSMACP
Sbjct: 439 VSFSSRGPNPATLDILKPDLAAPGVHILAAWPPISPISGVQGDTRAVLYTMQSGTSMACP 498

Query: 364 HAT-------------AAAAYVKSFHPTWSPAA------------------LKSA----- 423
           HAT             + AA   +   T  P +                  LKS      
Sbjct: 499 HATGAAAYIKSFHPTWSPAAIKSALMTTALPMSAEKNPDAEFAYGAGQIDPLKSVNPGLV 558

Query: 424 -----------LITTGYTTELLQRVSEDNNTCSSNNSNTVFDLNYPSFAFSTNISKPINH 479
                      L   GYTT+ LQ V+ DN+ CS   + TV+DLNYPSFA S++  + I  
Sbjct: 559 YDADKIDYVKFLCGQGYTTQTLQLVTGDNSVCSEATNGTVWDLNYPSFALSSSTFESITG 618

[Close]
BLAST of Lsi04G001550 vs. TrEMBL
Match: W9QVI7_9ROSA (Uncharacterized protein OS=Morus notabilis GN=L484_016738 PE=4 SV=1)

HSP 1 Score: 459.5 bits (1181), Expect = 5.0e-126
Identity = 271/538 (50.37%), Postives = 345/538 (64.13%), Query Frame = 1 

Query: 4   KIIGARSYRSDGKHPEGDIKGPRDSDGHGTHTASIVAGGLVRQSSMLGLALGTARGGVPS 63
           KIIGA+ Y+SDG   +G+I  P DS+GHGTHTAS  AGGLV  +S  G  LGTARGGVPS
Sbjct: 150 KIIGAKYYKSDGFFVKGEILSPLDSNGHGTHTASAAAGGLVSGASFFGFGLGTARGGVPS 209

Query: 64  ARIAAYKVCWSD-GCTDADILAAFDDAIADSVDIISGSLGGSRVRDYFNDSLAIGSFHAM 123
           AR+A YKVCW D GC DADILAAFDDAIAD VDIIS SL GS  RDYF +S+A+G+FHAM
Sbjct: 210 ARLAVYKVCWPDEGCQDADILAAFDDAIADGVDIISVSL-GSYARDYFQNSIAVGAFHAM 269

Query: 124 KKGILTSLAVGNNGPDFTSIVNFSPWSLSVAASTTDRKFETKVELGDGREFNGVSINTFD 183
           +KGILTS + GN   D +S+ NFSPWS+SVAAST DR F T V+LG+ + + G SINTFD
Sbjct: 270 RKGILTSTSAGN---DPSSLTNFSPWSISVAASTMDRNFVTNVQLGNDKIYEGTSINTFD 329

Query: 184 INGKQIPLVYAGDIPK--APFDSSVSRMCFENTVDLELVKGKIVVCDSLAVPGGVVAVKG 243
           +  KQ P++Y GD P   A +DSS+SR CF +++D  LVKGKIV+CD   +  G  +  G
Sbjct: 330 MKNKQFPVIYGGDAPNTTANYDSSLSRYCFGDSLDKNLVKGKIVLCDGYTLGTGAQSA-G 389

Query: 244 AAGIIMQDDSSPDDTNSFPLPASHLGPKAGALILSYINSTK------------------- 303
           A G++M   +S    +++PLP S L  +  A +  Y+NS++                   
Sbjct: 390 AVGVVMSGGASGKLADTYPLPLSSLSLEDSAKLYIYLNSSRKPSASIWKSKEKSDKLVPY 449

Query: 304 ------RGPNPITPNILKPDLCGPGVEILAAWPPIASPSGAEEDR----------TSMAC 363
                 RGPNPITPNILKPDL  PG  ILAAWPP  S S  E D           TSMAC
Sbjct: 450 IASYSSRGPNPITPNILKPDLAAPGAYILAAWPPYISVSRIEGDNRIAAYNILSGTSMAC 509

Query: 364 PHATAAAAYVKSFHPTWSPAALKSALITTG--YTTELLQRV-----SEDNNTCSSNNSNT 423
           PHA+AAAAYVKSFHPTWSPAA+KS+L+TT    T ++         S   N   +     
Sbjct: 510 PHASAAAAYVKSFHPTWSPAAIKSSLMTTAKPMTADINSEAEFAYGSGLINPLKAAYPGL 569

Query: 424 VFDL---NYPSFAFS-------------TNISKPINHVYRRSVTNVGSKYATYKATIINP 480
           V+D+   +Y +F                T+  + I+HV+ R+VTNVGS  +TYKA ++ P
Sbjct: 570 VYDIDENDYVNFLCGRYNAKLLKIITGNTSPLESIDHVFNRTVTNVGSPTSTYKANLVAP 629

[Close]
BLAST of Lsi04G001550 vs. TAIR10
Match: AT5G59190.1 (AT5G59190.1 subtilase family protein)

HSP 1 Score: 360.9 bits (925), Expect = 1.2e-99
Identity = 228/560 (40.71%), Postives = 309/560 (55.18%), Query Frame = 1 

Query: 4   KIIGARSYRSDGKHPEGDIKGPRDSDGHGTHTASIVAGGLVRQSSMLGLALGTARGGVPS 63
           K+IGAR Y              RD +GHGTHTAS  AG  V+ +S  GLA GTARGGVPS
Sbjct: 140 KLIGARFYNKFAD-------SARDEEGHGTHTASTAAGNAVQAASFYGLAQGTARGGVPS 199

Query: 64  ARIAAYKVCWSDGCTDADILAAFDDAIADSVDIISGSLGGSRVRDYFNDSLAIGSFHAMK 123
           ARIAAYKVC++  C D DILAAFDDAIAD VD+IS S+    V +  N S+AIGSFHAM 
Sbjct: 200 ARIAAYKVCFNR-CNDVDILAAFDDAIADGVDVISISISADYVSNLLNASVAIGSFHAMM 259

Query: 124 KGILTSLAVGNNGPDFTSIVNFSPWSLSVAASTTDRKFETKVELGDGREFNGVSINTFDI 183
           +GI+T+ + GNNGPD  S+ N SPW ++VAAS TDR+F  +V LG+G+   G+S+NTF++
Sbjct: 260 RGIITAGSAGNNGPDQGSVANVSPWMITVAASGTDRQFIDRVVLGNGKALTGISVNTFNL 319

Query: 184 NGKQIPLVYAGDIPKAPFDSSVSRMCFENTVDLELVKGKIVVCDSLAVPGGVVAVKGAAG 243
           NG + P+VY  ++ +    +     C    VD ELVKGKIV+CD   +      + GA G
Sbjct: 320 NGTKFPIVYGQNVSRNCSQAQAG-YCSSGCVDSELVKGKIVLCDDF-LGYREAYLAGAIG 379

Query: 244 IIMQDDSSPDDTNSFPLPASHLGPKAGALILSYINSTK---------------------- 303
           +I+Q+   PD     P PAS LG +    I SYI S +                      
Sbjct: 380 VIVQNTLLPDSAFVVPFPASSLGFEDYKSIKSYIESAEPPQAEILRTEEIVDREAPYVPS 439

Query: 304 ---RGPNPITPNILKPDLCGPGVEILAAWPPIASPS---GAEEDR---------TSMACP 363
              RGP+ +  N+LKPD+  PG+EILAA+ P+ASPS     E+ R         TSMACP
Sbjct: 440 FSSRGPSFVIQNLLKPDVSAPGLEILAAFSPVASPSSFLNPEDKRSVRYSVMSGTSMACP 499

Query: 364 HATAAAAYVKSFHPTWSPAALKSALITTGYTTELLQRVSED-------NNTCSSNNSNTV 423
           H    AAYVKSFHP WSP+A+KSA++TT     L +   ++        N   +++   V
Sbjct: 500 HVAGVAAYVKSFHPDWSPSAIKSAIMTTATPMNLKKNPEQEFAYGSGQINPTKASDPGLV 559

Query: 424 FDLNYPSFA------------------------------------FSTNISK--PINHVY 480
           +++    +                                      +T +S   P N  +
Sbjct: 560 YEVETEDYLKMLCAEGFDSTTLTTTSGQNVTCSERTEVKDLNYPTMTTFVSSLDPFNVTF 619

[Close]
BLAST of Lsi04G001550 vs. TAIR10
Match: AT4G00230.1 (AT4G00230.1 xylem serine peptidase 1)

HSP 1 Score: 319.7 bits (818), Expect = 3.1e-87
Identity = 181/402 (45.02%), Postives = 247/402 (61.44%), Query Frame = 1 

Query: 4   KIIGARSYRSDGKHPEGDIKGPRDSDGHGTHTASIVAGGLVRQSSMLGLALGTARGGVPS 63
           KIIGA+ ++ DG  P G+++ P D DGHGTHT+S VAG LV  +S+ G+A GTARG VPS
Sbjct: 183 KIIGAKYFKHDGNVPAGEVRSPIDIDGHGTHTSSTVAGVLVANASLYGIANGTARGAVPS 242

Query: 64  ARIAAYKVCWS-DGCTDADILAAFDDAIADSVDIISGSLGGSRVRDYFNDSLAIGSFHAM 123
           AR+A YKVCW+  GC D DILA F+ AI D V+IIS S+GG  + DY +DS+++GSFHAM
Sbjct: 243 ARLAMYKVCWARSGCADMDILAGFEAAIHDGVEIISISIGGP-IADYSSDSISVGSFHAM 302

Query: 124 KKGILTSLAVGNNGPDFTSIVNFSPWSLSVAASTTDRKFETKVELGDGREFNGVSINTFD 183
           +KGILT  + GN+GP   ++ N  PW L+VAAS  DR F++K++LG+G+ F+G+ I+ F 
Sbjct: 303 RKGILTVASAGNDGPSSGTVTNHEPWILTVAASGIDRTFKSKIDLGNGKSFSGMGISMFS 362

Query: 184 INGKQIPLVYAGDIPKAPFDSSVSRMCFENTVDLELVKGKIVVCDSLAVPGGV---VAVK 243
              K  PLV   D  K   D  ++R CF +++D + VKGK++VC      GGV   +   
Sbjct: 363 PKAKSYPLVSGVDAAKNTDDKYLARYCFSDSLDRKKVKGKVMVCRMGG--GGVESTIKSY 422

Query: 244 GAAGIIMQDDSSPDDTNSFPLPASHLGPKAGALILSYINSTK------------------ 303
           G AG I+  D   D+   F  PA+ +    G +I  YINST+                  
Sbjct: 423 GGAGAIIVSDQYLDNAQIFMAPATSVNSSVGDIIYRYINSTRSASAVIQKTRQVTIPAPF 482

Query: 304 ------RGPNPITPNILKPDLCGPGVEILAAWPPIASPSGAEEDR----------TSMAC 363
                 RGPNP +  +LKPD+  PG++ILAA+    S +G + D           TSMAC
Sbjct: 483 VASFSSRGPNPGSIRLLKPDIAAPGIDILAAFTLKRSLTGLDGDTQFSKFTILSGTSMAC 542

Query: 364 PHATAAAAYVKSFHPTWSPAALKSALITTGYTTELLQRVSED 368
           PH    AAYVKSFHP W+PAA+KSA+IT+     + +RV++D
Sbjct: 543 PHVAGVAAYVKSFHPDWTPAAIKSAIITS--AKPISRRVNKD 579

[Close]
BLAST of Lsi04G001550 vs. TAIR10
Match: AT5G03620.1 (AT5G03620.1 Subtilisin-like serine endopeptidase family protein)

HSP 1 Score: 305.8 bits (782), Expect = 4.6e-83
Identity = 183/398 (45.98%), Postives = 244/398 (61.31%), Query Frame = 1 

Query: 4   KIIGARSY--RSDGKHPEGDIKGPRDSDGHGTHTASIVAGGLVRQSSMLGLALGTARGGV 63
           K+IGA+ +  +S+G  P+G+     D DGHGTHT+S +AG  V  +S+ G+A GTARGGV
Sbjct: 182 KVIGAKYFHIQSEGL-PDGEGDTAADHDGHGTHTSSTIAGVSVSSASLFGIANGTARGGV 241

Query: 64  PSARIAAYKVCWSDGCTDADILAAFDDAIADSVDIISGSLGGSRVRDYFNDSLAIGSFHA 123
           PSARIAAYKVCW  GCTD D+LAAFD+AI+D VDIIS S+GG+ +  +F D +AIG+FHA
Sbjct: 242 PSARIAAYKVCWDSGCTDMDMLAAFDEAISDGVDIISISIGGASL-PFFEDPIAIGAFHA 301

Query: 124 MKKGILTSLAVGNNGPDFTSIVNFSPWSLSVAASTTDRKFETKVELGDGREFNGVSINTF 183
           MK+GILT+ + GNNGP   ++ N +PW ++VAA++ DRKFET V+LG+G   +G+S+N F
Sbjct: 302 MKRGILTTCSAGNNGPGLFTVSNLAPWVMTVAANSLDRKFETVVKLGNGLTASGISLNGF 361

Query: 184 DINGKQIPLV---YAGDIPKAPFDSSVSRMCFENTVDLELVKGKIVVCDSLAVPGG---- 243
           +   K  PL     A ++    +    +  C   T+  + V GK+V C++    GG    
Sbjct: 362 NPRKKMYPLTSGSLASNLSAGGYGEPST--CEPGTLGEDKVMGKVVYCEAGREEGGNGGQ 421

Query: 244 ----VVAVKGAAGIIMQDDSSPDDTNSFPLPASHLGPKAGALILSYINSTK--------- 303
               VV     AG+I+Q     D   S  +  S++  + G  I  YINSTK         
Sbjct: 422 GQDHVVRSLKGAGVIVQLLEPTDMATSTLIAGSYVFFEDGTKITEYINSTKNPQAVIFKT 481

Query: 304 ---------------RGPNPITPNILKPDLCGPGVEILAAWPPIASPSGAEEDR------ 355
                          RGP  I+PNILKPD+  PG+ ILAA+  +AS +G  +D       
Sbjct: 482 KTTKMLAPSISSFSARGPQRISPNILKPDISAPGLNILAAYSKLASVTGYPDDNRRTLFS 541

[Close]
BLAST of Lsi04G001550 vs. TAIR10
Match: AT5G67360.1 (AT5G67360.1 Subtilase family protein)

HSP 1 Score: 301.2 bits (770), Expect = 1.1e-81
Identity = 213/584 (36.47%), Postives = 296/584 (50.68%), Query Frame = 1 

Query: 4   KIIGARSYRSDGKHPEGDI------KGPRDSDGHGTHTASIVAGGLVRQSSMLGLALGTA 63
           K+IGAR +    +   G I      + PRD DGHGTHT+S  AG +V  +S+LG A GTA
Sbjct: 179 KLIGARFFARGYESTMGPIDESKESRSPRDDDGHGTHTSSTAAGSVVEGASLLGYASGTA 238

Query: 64  RGGVPSARIAAYKVCWSDGCTDADILAAFDDAIADSVDIISGSLGGSRVRDYFNDSLAIG 123
           RG  P AR+A YKVCW  GC  +DILAA D AIAD+V+++S SLGG  + DY+ D +AIG
Sbjct: 239 RGMAPRARVAVYKVCWLGGCFSSDILAAIDKAIADNVNVLSMSLGGG-MSDYYRDGVAIG 298

Query: 124 SFHAMKKGILTSLAVGNNGPDFTSIVNFSPWSLSVAASTTDRKFETKVELGDGREFNGVS 183
           +F AM++GIL S + GN GP  +S+ N +PW  +V A T DR F     LG+G+ F GVS
Sbjct: 299 AFAAMERGILVSCSAGNAGPSSSSLSNVAPWITTVGAGTLDRDFPALAILGNGKNFTGVS 358

Query: 184 INTFD-INGKQIPLVYAGDIPKAPFDSSVSRMCFENTVDLELVKGKIVVCD----SLAVP 243
           +   + +  K +P +YAG+   A    +   +C   T+  E VKGKIV+CD    +    
Sbjct: 359 LFKGEALPDKLLPFIYAGNASNA----TNGNLCMTGTLIPEKVKGKIVMCDRGINARVQK 418

Query: 244 GGVVAVKGAAGIIMQDDSSPDD---TNSFPLPASHLGPKAGALILSYINSTKRGPNP--- 303
           G VV   G  G+I+ + ++  +    ++  LPA+ +G KAG +I  Y+ +    PNP   
Sbjct: 419 GDVVKAAGGVGMILANTAANGEELVADAHLLPATTVGEKAGDIIRHYVTT---DPNPTAS 478

Query: 304 --------------------------ITPNILKPDLCGPGVEILAAWPPIASPSGAEEDR 363
                                     ITPNILKPDL  PGV ILAAW   A P+G   D 
Sbjct: 479 ISILGTVVGVKPSPVVAAFSSRGPNSITPNILKPDLIAPGVNILAAWTGAAGPTGLASDS 538

Query: 364 ----------TSMACPHATAAAAYVKSFHPTWSPAALKSALITTGYTT-----ELLQRVS 423
                     TSM+CPH +  AA +KS HP WSPAA++SAL+TT Y T      LL   +
Sbjct: 539 RRVEFNIISGTSMSCPHVSGLAALLKSVHPEWSPAAIRSALMTTAYKTYKDGKPLLDIAT 598

Query: 424 EDNNT-----------CSSNNSNTVFDL---NYPSFAFSTNISKP-INHVYRRSVTNVGS 479
              +T            ++ N   ++DL   +Y  F  + N + P I  V RR+ T   S
Sbjct: 599 GKPSTPFDHGAGHVSPTTATNPGLIYDLTTEDYLGFLCALNYTSPQIRSVSRRNYTCDPS 658

[Close]
BLAST of Lsi04G001550 vs. TAIR10
Match: AT3G46850.1 (AT3G46850.1 Subtilase family protein)

HSP 1 Score: 296.6 bits (758), Expect = 2.8e-80
Identity = 177/388 (45.62%), Postives = 228/388 (58.76%), Query Frame = 1 

Query: 4   KIIGARSYRSDGKHPEGDIKGPRDSDGHGTHTASIVAGGLVRQSSMLGLALGTARGGVPS 63
           K+IGAR Y       EG  +  RD+ GHG+HTASI AG  V+  S  GL  GT RGGVP+
Sbjct: 180 KLIGARYYTPK---LEGFPESARDNTGHGSHTASIAAGNAVKHVSFYGLGNGTVRGGVPA 239

Query: 64  ARIAAYKVCWSD--GCTDADILAAFDDAIADSVDIISGSLGGSRVRDYFNDSLAIGSFHA 123
           ARIA YKVC      CT   ILAAFDDAIAD VDII+ SLG   V  +  D+LAIG+FHA
Sbjct: 240 ARIAVYKVCDPGVIRCTSDGILAAFDDAIADKVDIITVSLGADAVGTFEEDTLAIGAFHA 299

Query: 124 MKKGILTSLAVGNNGPDFTSIVNFSPWSLSVAASTTDRKFETKVELGDGREFNGVSINTF 183
           M KGILT    GNNGP+  +IV+ +PW  +VAAS  +R F TKV LG+G+   G S+N+F
Sbjct: 300 MAKGILTVNGAGNNGPERRTIVSMAPWLFTVAASNMNRAFITKVVLGNGKTIVGRSVNSF 359

Query: 184 DINGKQIPLVYAGDIPKAPFDSSVSRMCFENTVDLELVKGKIVVCDSLAVPGGVVAVKGA 243
           D+NGK+ PLVY G    +  D+S +  C    +D + VKGKIV+CD+   PG   A+   
Sbjct: 360 DLNGKKYPLVY-GKSASSRCDASSAGFCSPGCLDSKRVKGKIVLCDTQRNPGEAQAMGAV 419

Query: 244 AGIIMQDDSSPDDTNSFPLPASHLGPKAGALILSYINSTK-------------------- 303
           A I+   +   D  + F  P S L      ++LSY+NSTK                    
Sbjct: 420 ASIVR--NPYEDAASVFSFPVSVLSEDDYNIVLSYVNSTKNPKAAVLKSETIFNQKAPVV 479

Query: 304 -----RGPNPITPNILKPDLCGPGVEILAAWPPIASPSGAEE--------DRTSMACPHA 357
                RGPNP+  +ILKPD+  PG EILAA+ P   PS ++           TSM+CPH 
Sbjct: 480 ASYSSRGPNPLIHDILKPDITAPGSEILAAYSPYVPPSESDTRHVKYTVISGTSMSCPHV 539

[Close]
BLAST of Lsi04G001550 vs. NCBI nr
Match: gi|700197004|gb|KGN52181.1| (hypothetical protein Csa_5G613590 [Cucumis sativus])

HSP 1 Score: 730.3 bits (1884), Expect = 2.2e-207
Identity = 395/567 (69.66%), Postives = 435/567 (76.72%), Query Frame = 1 

Query: 4   KIIGARSYRSDGKHPEGDIKGPRDSDGHGTHTASIVAGGLVRQSSMLGLALGTARGGVPS 63
           KIIGARSYRS+G++PEGDIKGPRDSDGHGTHTASIVAGGLVR++SMLGL LGTARGGVPS
Sbjct: 177 KIIGARSYRSNGEYPEGDIKGPRDSDGHGTHTASIVAGGLVRRASMLGLGLGTARGGVPS 236

Query: 64  ARIAAYKVCWSDGCTDADILAAFDDAIADSVDIISGSLGGSRVRDYFNDSLAIGSFHAMK 123
           ARIAAYKVCWSDGC+DADILAAFDDAIAD VDIISGSLGGS  RDYFNDS+AIGSFHAMK
Sbjct: 237 ARIAAYKVCWSDGCSDADILAAFDDAIADGVDIISGSLGGSGARDYFNDSIAIGSFHAMK 296

Query: 124 KGILTSLAVGNNGPDFTSIVNFSPWSLSVAASTTDRKFETKVELGDGREFNGVSINTFDI 183
           KGILTSLAVGNNGPDFT+IVNFSPWSLSVAASTTDRKFETKVELGDGREF+GVS+NTFDI
Sbjct: 297 KGILTSLAVGNNGPDFTTIVNFSPWSLSVAASTTDRKFETKVELGDGREFSGVSVNTFDI 356

Query: 184 NGKQIPLVYAGDIPKAPFDSSVSRMCFENTVDLELVKGKIVVCDSLAVPGGVVAVKGAAG 243
            GKQIPLVYAGDIPKAPFDSSVSR+CFENTVDL+LVKGKIVVCDSL VPGGVVAVKGA G
Sbjct: 357 KGKQIPLVYAGDIPKAPFDSSVSRLCFENTVDLKLVKGKIVVCDSLTVPGGVVAVKGAVG 416

Query: 244 IIMQDDSSPDDTNSFPLPASHLGPKAGALILSYINST----------------------- 303
           IIMQDDSS DDTNSFP+PASHLGPKAGAL+LSYINST                       
Sbjct: 417 IIMQDDSSHDDTNSFPIPASHLGPKAGALVLSYINSTNSIPTATIKKSTERKRKRAPSVA 476

Query: 304 ---KRGPNPITPNILKPDLCGPGVEILAAWPPIASPSGAEEDR----------TSMACPH 363
               RGPNPITPNILKPDL GPGVEILAAW P++ PSGAEED           TSMACPH
Sbjct: 477 SFSSRGPNPITPNILKPDLSGPGVEILAAWSPVSPPSGAEEDNKRVLYNIISGTSMACPH 536

Query: 364 ATAAAAYVKSFHPTWSPAALKSALITTGYTTELLQRVSED-------NNTCSSNNSNTVF 423
            TAAAAYVKSFHPTWSP+ALKSALITT +         ++        N   + +   ++
Sbjct: 537 VTAAAAYVKSFHPTWSPSALKSALITTAFPMSPKHNPDKEFGYGAGHINPLGAVHPGLIY 596

Query: 424 D---LNYPSF----AFSTNISK------------------PINHVYRRSVTNVG------ 483
           D   ++Y  F     ++T + +                   +N+      TN+       
Sbjct: 597 DASEIDYVQFLCGQGYTTELLQLVSEDNNTCSSNNSDTVFDLNYPSFALSTNISKPINQV 656

Query: 484 ---------SKYATYKATIINPWKNLEIKVKPSILSFKNLGEEQSFELTIKGRISKNIES 487
                    SKYATYKAT+INPWKNLEIKV PS+LSFKNLGE+QSFE+TI+G+I K+IES
Sbjct: 657 YKRTVTNVGSKYATYKATVINPWKNLEIKVNPSVLSFKNLGEKQSFEVTIRGKIRKDIES 716

[Close]
BLAST of Lsi04G001550 vs. NCBI nr
Match: gi|659091821|ref|XP_008446751.1| (PREDICTED: cucumisin-like [Cucumis melo])

HSP 1 Score: 717.6 bits (1851), Expect = 1.5e-203
Identity = 386/551 (70.05%), Postives = 423/551 (76.77%), Query Frame = 1 

Query: 4   KIIGARSYRSDGKHPEGDIKGPRDSDGHGTHTASIVAGGLVRQSSMLGLALGTARGGVPS 63
           KIIGARSYRS+G++PEGDIKGPRDSDGHGTHTASIVAGGLVRQ++MLGL LGTARGGVPS
Sbjct: 176 KIIGARSYRSNGEYPEGDIKGPRDSDGHGTHTASIVAGGLVRQANMLGLGLGTARGGVPS 235

Query: 64  ARIAAYKVCWSDGCTDADILAAFDDAIADSVDIISGSLGGSRVRDYFNDSLAIGSFHAMK 123
           ARIAAYKVCWSDGC+DADILAAFDDAIAD VDIISGSLGGS  RDYFNDS+AIGSFHAMK
Sbjct: 236 ARIAAYKVCWSDGCSDADILAAFDDAIADGVDIISGSLGGSGFRDYFNDSIAIGSFHAMK 295

Query: 124 KGILTSLAVGNNGPDFTSIVNFSPWSLSVAASTTDRKFETKVELGDGREFNGVSINTFDI 183
           KGILTSLAVGNNGPDFT+IVNFSPWSLSVAASTTDRKFETKVELGDGREFNGVS+NTFDI
Sbjct: 296 KGILTSLAVGNNGPDFTTIVNFSPWSLSVAASTTDRKFETKVELGDGREFNGVSVNTFDI 355

Query: 184 NGKQIPLVYAGDIPKAPFDSSVSRMCFENTVDLELVKGKIVVCDSLAVPGGVVAVKGAAG 243
            GKQIPLVYAGDIPKAPFDSSVSR+CFENT+DL+LVKGKIVVCDSL VPGGV AVKGA G
Sbjct: 356 KGKQIPLVYAGDIPKAPFDSSVSRLCFENTIDLKLVKGKIVVCDSLTVPGGVAAVKGAVG 415

Query: 244 IIMQDDSSPDDTNSFPLPASHLGPKAGALILSYINST----------------------- 303
           IIMQDDSSPDDTNSFP+PASHLGP+AGALILSYINST                       
Sbjct: 416 IIMQDDSSPDDTNSFPIPASHLGPEAGALILSYINSTNSIPTATIKKSTERKRKRAPSVA 475

Query: 304 ---KRGPNPITPNILKPDLCGPGVEILAAWPPIASPSGAEEDR----------TSMACPH 363
               RGPNPITPNILKPDL GPGVEILAAW PI+ PSGAEED           TSMACPH
Sbjct: 476 SFSSRGPNPITPNILKPDLSGPGVEILAAWSPISPPSGAEEDSKRVLYNIISGTSMACPH 535

Query: 364 ATAAAAYVKSFHPTWSPAALKSALITTGYTTELLQRVSED-------NNTCSSNNSNTVF 423
            TAAAAYVKSFHPTWSP+ALKSALITT ++      + ++        N   + +   ++
Sbjct: 536 VTAAAAYVKSFHPTWSPSALKSALITTAFSMSSKHNLDKEFGYGAGHINPLGAVHPGLIY 595

Query: 424 D---LNYPSF----AFSTNISKPI------------------NHVYRRSVTNV------- 472
           D   ++Y  F     ++T + + +                  N+      TN+       
Sbjct: 596 DASEIDYVHFLCGQGYTTELLQQVSEDNNTCSSNNSDTVFDLNYPSFALSTNISKPINQV 655

[Close]
BLAST of Lsi04G001550 vs. NCBI nr
Match: gi|778708693|ref|XP_004135365.2| (PREDICTED: LOW QUALITY PROTEIN: cucumisin-like [Cucumis sativus])

HSP 1 Score: 709.1 bits (1829), Expect = 5.2e-201
Identity = 381/551 (69.15%), Postives = 421/551 (76.41%), Query Frame = 1 

Query: 4   KIIGARSYRSDGKHPEGDIKGPRDSDGHGTHTASIVAGGLVRQSSMLGLALGTARGGVPS 63
           KIIGARSYRS+G++PEGDIKGPRDSDGHGTHTASIVAGGLVR++SMLGL LGTARGGVPS
Sbjct: 177 KIIGARSYRSNGEYPEGDIKGPRDSDGHGTHTASIVAGGLVRRASMLGLGLGTARGGVPS 236

Query: 64  ARIAAYKVCWSDGCTDADILAAFDDAIADSVDIISGSLGGSRVRDYFNDSLAIGSFHAMK 123
           ARIAAYKVCWSDGC+DADILAAFDDAIAD VDIISGSLGGS  RDYFNDS+AIGSFHAMK
Sbjct: 237 ARIAAYKVCWSDGCSDADILAAFDDAIADGVDIISGSLGGSGARDYFNDSIAIGSFHAMK 296

Query: 124 KGILTSLAVGNNGPDFTSIVNFSPWSLSVAASTTDRKFETKVELGDGREFNGVSINTFDI 183
           KGILTSLAVGNNGPDFT+IVNFSPWSLSVAASTTDRKFETKVELGDGREF+GVS+NTFDI
Sbjct: 297 KGILTSLAVGNNGPDFTTIVNFSPWSLSVAASTTDRKFETKVELGDGREFSGVSVNTFDI 356

Query: 184 NGKQIPLVYAGDIPKAPFDSSVSRMCFENTVDLELVKGKIVVCDSLAVPGGVVAVKGAAG 243
            GKQIPLVYAGDIPKAPFDSSVSR+CFENTVDL+LVKGKIVVCDSL VPGGVVAVKGA G
Sbjct: 357 KGKQIPLVYAGDIPKAPFDSSVSRLCFENTVDLKLVKGKIVVCDSLTVPGGVVAVKGAVG 416

Query: 244 IIMQDDSSPDDTNSFPLPASHLGPKAGALILSYINST----------------------- 303
           IIMQDDSS DDTNSFP+PASHLGPKAGAL+LSYINST                       
Sbjct: 417 IIMQDDSSHDDTNSFPIPASHLGPKAGALVLSYINSTNSIPTATIKKSTERKRKRAPSVA 476

Query: 304 ---KRGPNPITPNILKPDLCGPGVEILAAWPPIASPSGAEEDR----------TSMACPH 363
               RGPNPITPNILKPDL GPGVEILAAW P++ PSGAEED           TSMACPH
Sbjct: 477 SFSSRGPNPITPNILKPDLSGPGVEILAAWSPVSPPSGAEEDNKRVLYNIISGTSMACPH 536

Query: 364 ATAAAAYVKSFHPTWSPAALKSALITTGYTTELLQRVSED-------NNTCSSNNSNTVF 423
            TAAAAYVKSFHPTWSP+ALKSALITT +         ++        N   + +   ++
Sbjct: 537 VTAAAAYVKSFHPTWSPSALKSALITTAFPMSPKHNPDKEFGYGAGHINPLGAVHPGLIY 596

Query: 424 D---LNYPSF----AFSTNISK------------------PINHVYRRSVTNVG------ 472
           D   ++Y  F     ++T + +                   +N+      TN+       
Sbjct: 597 DASEIDYVQFLCGQGYTTELLQLVSEDNNTCSSNNSDTVFDLNYPSFALSTNISKPINQV 656

[Close]
BLAST of Lsi04G001550 vs. NCBI nr
Match: gi|659074279|ref|XP_008437519.1| (PREDICTED: cucumisin-like isoform X1 [Cucumis melo])

HSP 1 Score: 546.6 bits (1407), Expect = 4.4e-152
Identity = 312/564 (55.32%), Postives = 373/564 (66.13%), Query Frame = 1 

Query: 4   KIIGARSYRSDGKHPEGDIKGPRDSDGHGTHTASIVAGGLVRQSSMLGLALGTARGGVPS 63
           KIIGA+SYRSDG++P  DIKGPRDS+GHGTH AS VAGGLVRQ+SMLGL  GTARGGVPS
Sbjct: 180 KIIGAQSYRSDGRYPIDDIKGPRDSNGHGTHAASTVAGGLVRQASMLGLGTGTARGGVPS 239

Query: 64  ARIAAYKVCWSDGCTDADILAAFDDAIADSVDIISGSLGGSR-VRDYFNDSLAIGSFHAM 123
           ARIAAYKVCWSD C+ AD+LAAFDDAIAD VDIIS S+G  R + +YFND +AIG+FHAM
Sbjct: 240 ARIAAYKVCWSDTCSGADVLAAFDDAIADGVDIISLSVGPKRPIPNYFNDPIAIGTFHAM 299

Query: 124 KKGILTSLAVGNNGPDFTSIVNFSPWSLSVAASTTDRKFETKVELGDGREFNGVSINTFD 183
           + GILTS + GN GP   ++ NFSPW+LSVAASTTDR+F T V+LGDGR+FNGV+INTFD
Sbjct: 300 RNGILTSSSAGNEGPRPFTLTNFSPWALSVAASTTDRRFLTAVQLGDGRKFNGVTINTFD 359

Query: 184 INGKQIPLVYAGDIPKAP--FDSSVSRMCFENTVDLELVKGKIVVCDSLAVPGGVVAVKG 243
           +NG Q PLV+AG+IP     F+ S+SR C  N+VD ELVKGKI VCDS+ +P  V +++ 
Sbjct: 360 LNGTQYPLVHAGNIPNVTGGFNGSISRFCLGNSVDRELVKGKIAVCDSIVLPSDVGSLES 419

Query: 244 AAGIIMQDDSSPDDTNSFPLPASHLGPKAGALILSYINSTK------------------- 303
           A GIIMQD S  D T +FPLPASHLG +   LI SY+N T+                   
Sbjct: 420 ALGIIMQDGSPKDLTAAFPLPASHLGTQQRPLISSYLNLTRIPTATILKSTELKLEAAPL 479

Query: 304 ------RGPNPITPNILKPDLCGPGVEILAAWPPIASPSGAEEDR----------TSMAC 363
                 RGPNP TP+ILKPD+ GPGVEILAAW PI SPS A+ D           TSMAC
Sbjct: 480 VASFSSRGPNPTTPDILKPDVIGPGVEILAAWSPIRSPSDAKGDNRKLLFNIISGTSMAC 539

Query: 364 PHATAA-----------------AAYVKSFHP----------------------TWSPAA 423
           PHATA                  +A + +  P                        +P  
Sbjct: 540 PHATAVAAYVKSFHPSWSPAALKSALITTAFPMRGDLYPEAEFAYGSGHINPLGAVNPGL 599

Query: 424 LKSA--------LITTGYTTELLQRVSEDNNTCSSNNSNTVFDLNYPSFAFSTNISKPIN 483
           + +A        L   GY T LL+ +++DN+TCS  NS  V+DLNY SFA  T IS P +
Sbjct: 600 IYNASEIDYIRFLCGEGYNTTLLRIITKDNSTCSPTNSGLVYDLNYHSFALFTYISTPFS 659

[Close]
BLAST of Lsi04G001550 vs. NCBI nr
Match: gi|659074283|ref|XP_008437522.1| (PREDICTED: cucumisin-like isoform X3 [Cucumis melo])

HSP 1 Score: 546.6 bits (1407), Expect = 4.4e-152
Identity = 312/564 (55.32%), Postives = 373/564 (66.13%), Query Frame = 1 

Query: 4   KIIGARSYRSDGKHPEGDIKGPRDSDGHGTHTASIVAGGLVRQSSMLGLALGTARGGVPS 63
           KIIGA+SYRSDG++P  DIKGPRDS+GHGTH AS VAGGLVRQ+SMLGL  GTARGGVPS
Sbjct: 121 KIIGAQSYRSDGRYPIDDIKGPRDSNGHGTHAASTVAGGLVRQASMLGLGTGTARGGVPS 180

Query: 64  ARIAAYKVCWSDGCTDADILAAFDDAIADSVDIISGSLGGSR-VRDYFNDSLAIGSFHAM 123
           ARIAAYKVCWSD C+ AD+LAAFDDAIAD VDIIS S+G  R + +YFND +AIG+FHAM
Sbjct: 181 ARIAAYKVCWSDTCSGADVLAAFDDAIADGVDIISLSVGPKRPIPNYFNDPIAIGTFHAM 240

Query: 124 KKGILTSLAVGNNGPDFTSIVNFSPWSLSVAASTTDRKFETKVELGDGREFNGVSINTFD 183
           + GILTS + GN GP   ++ NFSPW+LSVAASTTDR+F T V+LGDGR+FNGV+INTFD
Sbjct: 241 RNGILTSSSAGNEGPRPFTLTNFSPWALSVAASTTDRRFLTAVQLGDGRKFNGVTINTFD 300

Query: 184 INGKQIPLVYAGDIPKAP--FDSSVSRMCFENTVDLELVKGKIVVCDSLAVPGGVVAVKG 243
           +NG Q PLV+AG+IP     F+ S+SR C  N+VD ELVKGKI VCDS+ +P  V +++ 
Sbjct: 301 LNGTQYPLVHAGNIPNVTGGFNGSISRFCLGNSVDRELVKGKIAVCDSIVLPSDVGSLES 360

Query: 244 AAGIIMQDDSSPDDTNSFPLPASHLGPKAGALILSYINSTK------------------- 303
           A GIIMQD S  D T +FPLPASHLG +   LI SY+N T+                   
Sbjct: 361 ALGIIMQDGSPKDLTAAFPLPASHLGTQQRPLISSYLNLTRIPTATILKSTELKLEAAPL 420

Query: 304 ------RGPNPITPNILKPDLCGPGVEILAAWPPIASPSGAEEDR----------TSMAC 363
                 RGPNP TP+ILKPD+ GPGVEILAAW PI SPS A+ D           TSMAC
Sbjct: 421 VASFSSRGPNPTTPDILKPDVIGPGVEILAAWSPIRSPSDAKGDNRKLLFNIISGTSMAC 480

Query: 364 PHATAA-----------------AAYVKSFHP----------------------TWSPAA 423
           PHATA                  +A + +  P                        +P  
Sbjct: 481 PHATAVAAYVKSFHPSWSPAALKSALITTAFPMRGDLYPEAEFAYGSGHINPLGAVNPGL 540

Query: 424 LKSA--------LITTGYTTELLQRVSEDNNTCSSNNSNTVFDLNYPSFAFSTNISKPIN 483
           + +A        L   GY T LL+ +++DN+TCS  NS  V+DLNY SFA  T IS P +
Sbjct: 541 IYNASEIDYIRFLCGEGYNTTLLRIITKDNSTCSPTNSGLVYDLNYHSFALFTYISTPFS 600

The following BLAST results are available for this feature:
BLAST of Lsi04G001550 vs. Swiss-Prot
Match NameE-valueIdentityDescription
CUCM1_CUCME1.1e-10253.13Cucumisin OS=Cucumis melo PE=1 SV=1[more]
SBT43_ARATH2.2e-9840.71Subtilisin-like protease SBT4.3 OS=Arabidopsis thaliana GN=SBT4.3 PE=3 SV=1[more]
SBT4E_ARATH5.5e-8645.02Subtilisin-like protease SBT4.14 OS=Arabidopsis thaliana GN=SBT4.14 PE=2 SV=1[more]
SBT4F_ARATH8.2e-8245.98Subtilisin-like protease SBT4.15 OS=Arabidopsis thaliana GN=SBT4.15 PE=3 SV=1[more]
SBT17_ARATH2.0e-8036.47Subtilisin-like protease SBT1.7 OS=Arabidopsis thaliana GN=SBT1.7 PE=1 SV=1[more]
BLAST of Lsi04G001550 vs. TrEMBL
Match NameE-valueIdentityDescription
A0A0A0KWL9_CUCSA1.5e-20769.66Uncharacterized protein OS=Cucumis sativus GN=Csa_5G613590 PE=4 SV=1[more]
A0A0A0KJN5_CUCSA1.2e-15154.79Uncharacterized protein OS=Cucumis sativus GN=Csa_5G141040 PE=4 SV=1[more]
A0A0A0KRC4_CUCSA2.9e-14268.21Uncharacterized protein OS=Cucumis sativus GN=Csa_5G613580 PE=4 SV=1[more]
D7TLL6_VITVI2.5e-13350.45Putative uncharacterized protein OS=Vitis vinifera GN=VIT_13s0019g02480 PE=4 SV=... [more]
W9QVI7_9ROSA5.0e-12650.37Uncharacterized protein OS=Morus notabilis GN=L484_016738 PE=4 SV=1[more]
BLAST of Lsi04G001550 vs. TAIR10
Match NameE-valueIdentityDescription
AT5G59190.11.2e-9940.71 subtilase family protein[more]
AT4G00230.13.1e-8745.02 xylem serine peptidase 1[more]
AT5G03620.14.6e-8345.98 Subtilisin-like serine endopeptidase family protein[more]
AT5G67360.11.1e-8136.47 Subtilase family protein[more]
AT3G46850.12.8e-8045.62 Subtilase family protein[more]
BLAST of Lsi04G001550 vs. NCBI nr
Match NameE-valueIdentityDescription
gi|700197004|gb|KGN52181.1|2.2e-20769.66hypothetical protein Csa_5G613590 [Cucumis sativus][more]
gi|659091821|ref|XP_008446751.1|1.5e-20370.05PREDICTED: cucumisin-like [Cucumis melo][more]
gi|778708693|ref|XP_004135365.2|5.2e-20169.15PREDICTED: LOW QUALITY PROTEIN: cucumisin-like [Cucumis sativus][more]
gi|659074279|ref|XP_008437519.1|4.4e-15255.32PREDICTED: cucumisin-like isoform X1 [Cucumis melo][more]
gi|659074283|ref|XP_008437522.1|4.4e-15255.32PREDICTED: cucumisin-like isoform X3 [Cucumis melo][more]
Annotated Terms
The following terms have been associated with this gene:
Vocabulary: Biological Process
TermDefinition
GO:0006508proteolysis
Vocabulary: Molecular Function
TermDefinition
GO:0004252serine-type endopeptidase activity
Vocabulary: INTERPRO
TermDefinition
IPR015500Peptidase_S8_subtilisin-rel
IPR000209Peptidase_S8/S53_dom
GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0006508 proteolysis
cellular_component GO:0005575 cellular_component
molecular_function GO:0004252 serine-type endopeptidase activity
Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Lsi04G001550.1Lsi04G001550.1mRNA


InterPro
Analysis Name: InterPro Annotations of Lagenaria siceraria
Date Performed: 2017-09-18
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR000209Peptidase S8/S53 domainGENE3DG3DSA:3.40.50.200coord: 4..167
score: 1.6E-53coord: 272..358
score: 1.6
IPR000209Peptidase S8/S53 domainPFAMPF00082Peptidase_S8coord: 14..358
score: 3.4
IPR000209Peptidase S8/S53 domainunknownSSF52743Subtilisin-likecoord: 18..196
score: 5.63E-52coord: 276..358
score: 5.63
IPR015500Peptidase S8, subtilisin-relatedPRINTSPR00723SUBTILISINcoord: 27..40
score: 1.8E-5coord: 319..335
score: 1.
IPR015500Peptidase S8, subtilisin-relatedPANTHERPTHR10795PROPROTEIN CONVERTASE SUBTILISIN/KEXINcoord: 4..483
score: 8.1E
NoneNo IPR availablePANTHERPTHR10795:SF348SUBFAMILY NOT NAMEDcoord: 4..483
score: 8.1E

Synteny
The following gene(s) are orthologous to this gene:
GeneOrthologueOrganismBlock
Lsi04G001550Csa5G613580Cucumber (Chinese Long) v2culsiB368
Lsi04G001550CSPI05G26240Wild cucumber (PI 183967)cpilsiB372
Lsi04G001550Cucsa.282670Cucumber (Gy14) v1cgylsiB465
Lsi04G001550CmaCh04G014400Cucurbita maxima (Rimu)cmalsiB669
Lsi04G001550CmoCh04G015120Cucurbita moschata (Rifu)cmolsiB660
Lsi04G001550Cla020484Watermelon (97103) v1lsiwmB312
Lsi04G001550MELO3C012343Melon (DHL92) v3.5.1lsimeB281
Lsi04G001550ClCG05G021860Watermelon (Charleston Gray)lsiwcgB283
Lsi04G001550MELO3C012343.2Melon (DHL92) v3.6.1lsimedB297
Lsi04G001550Cla97C05G103560Watermelon (97103) v2lsiwmbB284
Lsi04G001550Bhi07G000629Wax gourdlsiwgoB387
Lsi04G001550Cp4.1LG01g14250Cucurbita pepo (Zucchini)cpelsiB352
Lsi04G001550CsGy5G025720Cucumber (Gy14) v2cgyblsiB337
Lsi04G001550CsaV3_5G035280Cucumber (Chinese Long) v3cuclsiB393
The following gene(s) are paralogous to this gene:

None

The following block(s) are covering this gene:
GeneOrganismBlock
Lsi04G001550Bottle gourd (USVL1VR-Ls)lsilsiB152
Lsi04G001550Cucurbita maxima (Rimu)cmalsiB379
Lsi04G001550Cucurbita maxima (Rimu)cmalsiB684
Lsi04G001550Cucurbita moschata (Rifu)cmolsiB364
Lsi04G001550Cucurbita moschata (Rifu)cmolsiB676
Lsi04G001550Cucurbita pepo (Zucchini)cpelsiB032
Lsi04G001550Cucurbita pepo (Zucchini)cpelsiB365
Lsi04G001550Watermelon (Charleston Gray)lsiwcgB300
Lsi04G001550Watermelon (97103) v1lsiwmB289
Lsi04G001550Silver-seed gourdcarlsiB767
Lsi04G001550Silver-seed gourdcarlsiB960