Cp4.1LG01g14250 (gene) Cucurbita pepo (Zucchini)

NameCp4.1LG01g14250
Typegene
OrganismCucurbita pepo (Cucurbita pepo (Zucchini))
DescriptionSubtilisin-like protease C1
LocationCp4.1LG01 : 7751256 .. 7755603 (+)
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: CDS
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGGCTTCTCTCTCAACAACCCTTTTATTCCTCAGTTTCTGCTTCTCTCTCCTCTTTTTCAGCTCATTTTCACAAGAAGACCCTCAAAAGGTAATTCTCTCTCTAAAAAATATTTTATTTTATTTATTATTTATTTCTAAATTTTTATTTATTTTATTTGGCAGACATATATTGTGTATATGGGAAGCCATCCAAAAGGGAAGGTTTCAACTTCATCTCACCATATAAGATTTTTAACAGAAACGATTGGCAGGTAATAATAATAATAATAATAATAATAATAATAATAATAATTCATCCCATTTTCGGAGTAATTCACAAATTTATTTATTTATTTTTAATTTTTTGCAGTAATTTTGCTCCACGGTCTTTAATCCGTAGCTACAAGAGAAGCTTCAATGGCTTTGTGGCTAAGCTGACTGAAGCCGAAGCCAAGAAAGTGTCGGGTATATTTCAAGGGCTGCCTTTATTATGAGTTTTTATTTATTTATTTATTTATTTATTTTTGTGTATTTAAACTCTGGTTTTAGAAATGGAAGGGGTAATTTCGGTATTTCCCAATGGAAAAAAACAACTCCACACTACAAGGTCTTGGGATTTCATGGGCTTCTCCGAACAAGTCAAGAGAATTCCGGCGGTGGAAAGTAACATCATTGTCGGAGTATTCGACAGCGGAATTTGGCCGGAATCTCCTAGCTTTGACGACACAGGATACGGTCCGCCGCCAGCCAAGTGGAGGGGCCGCTGTGAAGCCTCCGCCAATTTCTCTTGCAACAAGTAAGCTATATATATATATATTTATTTATTTTTTTAAAAAAAAATTATTTATTAACTTAGGGTCTAAATGGGATTTTTTTTTTTTTTTTTTAATAGTAAAATTATTGGAGCTAGATCGTATCGGAGTAATGGTAAACATTTAGAGAACGACATCAAAGGTCCAATAGATTCGGATGGCCATGGGACGCACACAGCATCGACAGTGGCTGGAGGGTTAGTCCGGCAAGCGAGTATGTTGGGTCTCGGCCTTGGCACGGCAAGGGGTGGAGTCCCATCAGCACGCATTGCTGCTTACAAAGTATGTTGGTCCGACGGCTGCACTGACGCTGATATTCTTGCAGCATTCGACGATGCCATTGCTGATGGTGTTGATATCATCTCTGGCTCTCTTGGGGGTTCGAGTGTGAAAGATTACTTCAATGACTCTATAGCCATTGGATCTTTCCATGCAATGAAGAAGGGAATCCTAACAACATTGGCCGTTGGAAATAACGGGCCGGAATTCACAACCATTGTGAACTTCTCGCCGTGGTCGTTGTCGGTAGCCGCTAGCACCACTGATCGGAAATTCGAAACTAAGGTTAAGCTCGGAGATGGAAGAGAATTCAACGTAAGAAAATTAATTAAGTTTTTCTCTCGTAAAAAGATTATGAAATTAAACGAAAATATTTTCATTTGGTTTATGAAGGGAGTCAGCGTCAATACATTTGACTTAAACGGAACACAAATTCCATTAGTTTATGCCGGAGATATCCCTAAAGCTCCATTCGACAGATCGGTGTCAAGGTAAATTATGAATTTATTTTAGAGAAAAATTAAATAAATAATTGAGTGTAGAGGTGAAAGGACTAATTTGACCTTGGAAAAACAGAATGTGCTTCGAGAACACAGTGGACAAGGAAAGGGTAAAAGGTAAAATTGTAGTATGTGATTCGTTGGCCGTTCCCGGCGGAGTTGTGGCCGTAGAAGGCGCCGCCGGAATTATAATGCAAGACGAATCCCCACAAGACGATACCAATTCTTATCCATTGCCTGCTTCTCATGTTGGCCCAAAACCTGGCGCTCTCATTCTCTCTTACATCAACTCAACCAGGTAAATCAATATTTACCTTAATTTATTTGTATTTTACCCTAATTGAATTTTACCCAAATACTCGACTTGAATTTATTGTTAATTTTAAATAATTTTTTTTCCTAGTCCATACATATATTTATTTTATTTTTATAATTAACTTTTTTTATTACTTATTTTCGAATAATTTTTAACTAATTAAAATATATATTCAATTATGTTGTAAATCTTAATTAAAATTTTAAAATAAATAAATAAATTTTACAAATTAAGATTTTACTTTATTAAAAAAAATTAAATAAATAACTCATCAACCCGAGTTATAAGACTTGTTTGAGTTGAAAAATTTGTATCTCCTATAATTAGGTTATATCTAAAAAAAAAAAATATCTACACCCAACCCACTAACACCGTAAGTCTACTAGTAGGGTTGTGATTGTTTTTACCGTATAGCTCCGTGATCGAACCACTGTTTGTCACATAGTAAATTTTATATCTTTTTAGAAAATATTTCAGACTTTTTTTTGGTAACAACTTAAACTCACCACGTTTGTTAGGAGAGGTTTTCACACTGTTGGGTAAAGAACGTTTCGTTCTACTCTCTAACCAACGTGGAATCTCACATTTTTCTTACTAGAATAGAGCCGTAAAAAAATGGGCACAGCCCAGGTATCGGGTATTAGAACACGTTAATAATTACATACATGATGATTTACAGGGTTCCAACAGCAAGTATAATGAAGAGCAGAGGGAGAAAACGTAATAGAGCCCCTTTTGTTGCATCATTTTCTTCAAGGGGTCCAAACCCAATAACCCCCAACATTCTGAAGGTAAATTATTAATTTAATTTAATTAATTGAATTATTATTATTATTATTATTATATTATTATTATATTAATTGTAAATGATTTATGCAGCCGGATATATCAGGGCCTGGCGTTGAAATCCTGGCGGCGTGGCCTTCCACGGTGTCACCCTCAGGAGCTGAAGAAGATAATAAAAGGGTTGTTTATAATGTGATTTCGGGCACCTCCATGGCTTGCCCGCATGTCACTGCCGCCGCCGCTTATGTTAAGTCCTTCCATCCCACTTGGTCTCCTGCTGCTCTTAAATCAGCCCTTATCACAACAGGTAATTAAATAGAACACAACTATTTTTCACTTTTTTATTTTAAGAATTATTAGCTAATTTAAATCATTTTTTCAAAAATGTTTTGGAATTACCGGGAAATTACATTTTTTTTTAATGTTATTTGTGTATTTAAATTTTGATTTTAAAATTTTAAAATGTATAATTATATATTAAAAAAAAAAGAAAATTTAATAAATATAAACTCGATTTATCAATCCAATGGAATTGGGTTGGATTGTGAATTTATTTAGGTTGGCCGAATTTAAATAAATAAAAATTTTATAAATTTAATTAATTTATAAATTTTTTATTCAATTAAATTAAATGTTCGTAATTTTTTTAAAAAAGATTTTTTTACACACAATTATATATATATATATATATATATATATTATTTTTTATTGTGTATTCTCACAACTACAAAAAAAAATAAAAAATAATAATAATAATAATGGTTAATTTTTGAAAATATATATAGACATGGTAAATTTTAATGAAATATTGGGAGATAAATAAATAAATAGATATTTTAAAATTTTAACATTTTATTTTTATTAAAAAATTTAAATAAATGAACTCCTGTCAAGTCTCAAAATGGTAGATATAGTACGGGAGTCATTTTCACCTATGCAAATCAAAATATAAGCTTTCACAGATAGAAGTTCATACCTCACTTATGATTAAGATCGAGTAGCATATGATCCTCATGTGAAATATTAATTTTTTCAATTAACGGATTTATGGAGATATAGATTACATCTTTGGAATAATTAGAATTAAACAAATCCAAACATTAAGTTATTATTTAATATATTAATGCAGCATTTCCAATGAGCCCCAAACGTAACCCAGACGGCGAGTTAGCGTACGGCGCGGGCCACATAAACCCACTGGGCGCAGTCCATCCAGGCTTAATCTACAACGCTTCAGAGACCGACTACGTGAAGTTTCTATGCGGTCAAGGCTACTCCACGGAGTCGCTCCGGCGACTCTCCGACGACAGTGCCTCTTGCTCAGCCAACAATTCCGGCACAGTTTTCGAACTCAACTACCCATCCTTCGCTCTTTCCACGAACGCCTCAAACCCCATCAGCCAAGTTTTCAGAAGAAGGGTCACAAATGTTGGGTCGAGCTATGCGACGTCGTATAAAGCCGTGATAGTTAACCCATGGAGGGATCTTAAAATTACGGTGAAGCCCTCGGTTCTTTTGTTCAAGAGGTTGGGAGAGGAGCTAAGGTTTGAGGTTAGAATTGGAGGAAAAATTAGGAAAGGTATTGAATCGGCGGCTTTGGTGTGGGATGATGGTAAGCATAAAGTGAGGAGTCCTATAACCGTCTTTAATGCTAATCTTCACTCTTTGGATTAA

mRNA sequence

ATGGCTTCTCTCTCAACAACCCTTTTATTCCTCAGTTTCTGCTTCTCTCTCCTCTTTTTCAGCTCATTTTCACAAGAAGACCCTCAAAAGACATATATTGTGTATATGGGAAGCCATCCAAAAGGGAAGGTTTCAACTTCATCTCACCATATAAGATTTTTAACAGAAACGATTGGCAGTAATTTTGCTCCACGGTCTTTAATCCGTAGCTACAAGAGAAGCTTCAATGGCTTTGTGGCTAAGCTGACTGAAGCCGAAGCCAAGAAAGTGTCGGAAATGGAAGGGGTAATTTCGGTATTTCCCAATGGAAAAAAACAACTCCACACTACAAGGTCTTGGGATTTCATGGGCTTCTCCGAACAAGTCAAGAGAATTCCGGCGGTGGAAAGTAACATCATTGTCGGAGTATTCGACAGCGGAATTTGGCCGGAATCTCCTAGCTTTGACGACACAGGATACGGTCCGCCGCCAGCCAAGTGGAGGGGCCGCTGTGAAGCCTCCGCCAATTTCTCTTGCAACAATAAAATTATTGGAGCTAGATCGTATCGGAGTAATGGTAAACATTTAGAGAACGACATCAAAGGTCCAATAGATTCGGATGGCCATGGGACGCACACAGCATCGACAGTGGCTGGAGGGTTAGTCCGGCAAGCGAGTATGTTGGGTCTCGGCCTTGGCACGGCAAGGGGTGGAGTCCCATCAGCACGCATTGCTGCTTACAAAGTATGTTGGTCCGACGGCTGCACTGACGCTGATATTCTTGCAGCATTCGACGATGCCATTGCTGATGGTGTTGATATCATCTCTGGCTCTCTTGGGGGTTCGAGTGTGAAAGATTACTTCAATGACTCTATAGCCATTGGATCTTTCCATGCAATGAAGAAGGGAATCCTAACAACATTGGCCGTTGGAAATAACGGGCCGGAATTCACAACCATTGTGAACTTCTCGCCGTGGTCGTTGTCGGTAGCCGCTAGCACCACTGATCGGAAATTCGAAACTAAGGTTAAGCTCGGAGATGGAAGAGAATTCAACGGAGTCAGCGTCAATACATTTGACTTAAACGGAACACAAATTCCATTAGTTTATGCCGGAGATATCCCTAAAGCTCCATTCGACAGATCGGTGTCAAGAATGTGCTTCGAGAACACAGTGGACAAGGAAAGGGTAAAAGGTAAAATTGTAGTATGTGATTCGTTGGCCGTTCCCGGCGGAGTTGTGGCCGTAGAAGGCGCCGCCGGAATTATAATGCAAGACGAATCCCCACAAGACGATACCAATTCTTATCCATTGCCTGCTTCTCATAACACGTTAATAATTACATACATGATGATTTACAGGGTTCCAACAGCAAGTATAATGAAGAGCAGAGGGAGAAAACGTAATAGAGCCCCTTTTGTTGCATCATTTTCTTCAAGGGGTCCAAACCCAATAACCCCCAACATTCTGAAGCCGGATATATCAGGGCCTGGCGTTGAAATCCTGGCGGCGTGGCCTTCCACGGTGTCACCCTCAGGAGCTGAAGAAGATAATAAAAGGGTTGTTTATAATGTGATTTCGGGCACCTCCATGGCTTGCCCGCATGTCACTGCCGCCGCCGCTTATGTTAAGTCCTTCCATCCCACTTGGTCTCCTGCTGCTCTTAAATCAGCCCTTATCACAACAGCATTTCCAATGAGCCCCAAACGTAACCCAGACGGCGAGTTAGCGTACGGCGCGGGCCACATAAACCCACTGGGCGCAGTCCATCCAGGCTTAATCTACAACGCTTCAGAGACCGACTACGTGAAGTTTCTATGCGGTCAAGGCTACTCCACGGAGTCGCTCCGGCGACTCTCCGACGACAGTGCCTCTTGCTCAGCCAACAATTCCGGCACAGTTTTCGAACTCAACTACCCATCCTTCGCTCTTTCCACGAACGCCTCAAACCCCATCAGCCAAGTTTTCAGAAGAAGGGTCACAAATGTTGGGTCGAGCTATGCGACGTCGTATAAAGCCGTGATAGTTAACCCATGGAGGGATCTTAAAATTACGGTGAAGCCCTCGGTTCTTTTGTTCAAGAGGTTGGGAGAGGAGCTAAGGTTTGAGGTTAGAATTGGAGGAAAAATTAGGAAAGGTATTGAATCGGCGGCTTTGGTGTGGGATGATGGTAAGCATAAAGTGAGGAGTCCTATAACCGTCTTTAATGCTAATCTTCACTCTTTGGATTAA

Coding sequence (CDS)

ATGGCTTCTCTCTCAACAACCCTTTTATTCCTCAGTTTCTGCTTCTCTCTCCTCTTTTTCAGCTCATTTTCACAAGAAGACCCTCAAAAGACATATATTGTGTATATGGGAAGCCATCCAAAAGGGAAGGTTTCAACTTCATCTCACCATATAAGATTTTTAACAGAAACGATTGGCAGTAATTTTGCTCCACGGTCTTTAATCCGTAGCTACAAGAGAAGCTTCAATGGCTTTGTGGCTAAGCTGACTGAAGCCGAAGCCAAGAAAGTGTCGGAAATGGAAGGGGTAATTTCGGTATTTCCCAATGGAAAAAAACAACTCCACACTACAAGGTCTTGGGATTTCATGGGCTTCTCCGAACAAGTCAAGAGAATTCCGGCGGTGGAAAGTAACATCATTGTCGGAGTATTCGACAGCGGAATTTGGCCGGAATCTCCTAGCTTTGACGACACAGGATACGGTCCGCCGCCAGCCAAGTGGAGGGGCCGCTGTGAAGCCTCCGCCAATTTCTCTTGCAACAATAAAATTATTGGAGCTAGATCGTATCGGAGTAATGGTAAACATTTAGAGAACGACATCAAAGGTCCAATAGATTCGGATGGCCATGGGACGCACACAGCATCGACAGTGGCTGGAGGGTTAGTCCGGCAAGCGAGTATGTTGGGTCTCGGCCTTGGCACGGCAAGGGGTGGAGTCCCATCAGCACGCATTGCTGCTTACAAAGTATGTTGGTCCGACGGCTGCACTGACGCTGATATTCTTGCAGCATTCGACGATGCCATTGCTGATGGTGTTGATATCATCTCTGGCTCTCTTGGGGGTTCGAGTGTGAAAGATTACTTCAATGACTCTATAGCCATTGGATCTTTCCATGCAATGAAGAAGGGAATCCTAACAACATTGGCCGTTGGAAATAACGGGCCGGAATTCACAACCATTGTGAACTTCTCGCCGTGGTCGTTGTCGGTAGCCGCTAGCACCACTGATCGGAAATTCGAAACTAAGGTTAAGCTCGGAGATGGAAGAGAATTCAACGGAGTCAGCGTCAATACATTTGACTTAAACGGAACACAAATTCCATTAGTTTATGCCGGAGATATCCCTAAAGCTCCATTCGACAGATCGGTGTCAAGAATGTGCTTCGAGAACACAGTGGACAAGGAAAGGGTAAAAGGTAAAATTGTAGTATGTGATTCGTTGGCCGTTCCCGGCGGAGTTGTGGCCGTAGAAGGCGCCGCCGGAATTATAATGCAAGACGAATCCCCACAAGACGATACCAATTCTTATCCATTGCCTGCTTCTCATAACACGTTAATAATTACATACATGATGATTTACAGGGTTCCAACAGCAAGTATAATGAAGAGCAGAGGGAGAAAACGTAATAGAGCCCCTTTTGTTGCATCATTTTCTTCAAGGGGTCCAAACCCAATAACCCCCAACATTCTGAAGCCGGATATATCAGGGCCTGGCGTTGAAATCCTGGCGGCGTGGCCTTCCACGGTGTCACCCTCAGGAGCTGAAGAAGATAATAAAAGGGTTGTTTATAATGTGATTTCGGGCACCTCCATGGCTTGCCCGCATGTCACTGCCGCCGCCGCTTATGTTAAGTCCTTCCATCCCACTTGGTCTCCTGCTGCTCTTAAATCAGCCCTTATCACAACAGCATTTCCAATGAGCCCCAAACGTAACCCAGACGGCGAGTTAGCGTACGGCGCGGGCCACATAAACCCACTGGGCGCAGTCCATCCAGGCTTAATCTACAACGCTTCAGAGACCGACTACGTGAAGTTTCTATGCGGTCAAGGCTACTCCACGGAGTCGCTCCGGCGACTCTCCGACGACAGTGCCTCTTGCTCAGCCAACAATTCCGGCACAGTTTTCGAACTCAACTACCCATCCTTCGCTCTTTCCACGAACGCCTCAAACCCCATCAGCCAAGTTTTCAGAAGAAGGGTCACAAATGTTGGGTCGAGCTATGCGACGTCGTATAAAGCCGTGATAGTTAACCCATGGAGGGATCTTAAAATTACGGTGAAGCCCTCGGTTCTTTTGTTCAAGAGGTTGGGAGAGGAGCTAAGGTTTGAGGTTAGAATTGGAGGAAAAATTAGGAAAGGTATTGAATCGGCGGCTTTGGTGTGGGATGATGGTAAGCATAAAGTGAGGAGTCCTATAACCGTCTTTAATGCTAATCTTCACTCTTTGGATTAA

Protein sequence

MASLSTTLLFLSFCFSLLFFSSFSQEDPQKTYIVYMGSHPKGKVSTSSHHIRFLTETIGSNFAPRSLIRSYKRSFNGFVAKLTEAEAKKVSEMEGVISVFPNGKKQLHTTRSWDFMGFSEQVKRIPAVESNIIVGVFDSGIWPESPSFDDTGYGPPPAKWRGRCEASANFSCNNKIIGARSYRSNGKHLENDIKGPIDSDGHGTHTASTVAGGLVRQASMLGLGLGTARGGVPSARIAAYKVCWSDGCTDADILAAFDDAIADGVDIISGSLGGSSVKDYFNDSIAIGSFHAMKKGILTTLAVGNNGPEFTTIVNFSPWSLSVAASTTDRKFETKVKLGDGREFNGVSVNTFDLNGTQIPLVYAGDIPKAPFDRSVSRMCFENTVDKERVKGKIVVCDSLAVPGGVVAVEGAAGIIMQDESPQDDTNSYPLPASHNTLIITYMMIYRVPTASIMKSRGRKRNRAPFVASFSSRGPNPITPNILKPDISGPGVEILAAWPSTVSPSGAEEDNKRVVYNVISGTSMACPHVTAAAAYVKSFHPTWSPAALKSALITTAFPMSPKRNPDGELAYGAGHINPLGAVHPGLIYNASETDYVKFLCGQGYSTESLRRLSDDSASCSANNSGTVFELNYPSFALSTNASNPISQVFRRRVTNVGSSYATSYKAVIVNPWRDLKITVKPSVLLFKRLGEELRFEVRIGGKIRKGIESAALVWDDGKHKVRSPITVFNANLHSLD
BLAST of Cp4.1LG01g14250 vs. Swiss-Prot
Match: CUCM1_CUCME (Cucumisin OS=Cucumis melo PE=1 SV=1)

HSP 1 Score: 783.1 bits (2021), Expect = 2.7e-225
Identity = 408/735 (55.51%), Postives = 528/735 (71.84%), Query Frame = 1

Query: 4   LSTTLLFLSFCFSLLFFSSF-----SQEDPQKTYIVYMGSHPKGKVSTSSHHIRFLTETI 63
           +S++L+F  F FSL F +       S +D +  YIVYMG   +   S   HH   L + +
Sbjct: 1   MSSSLIFKLFFFSLFFSNRLASRLDSDDDGKNIYIVYMGRKLEDPDSAHLHHRAMLEQVV 60

Query: 64  GSNFAPRSLIRSYKRSFNGFVAKLTEAEAKKVSEMEGVISVFPNGKKQLHTTRSWDFMGF 123
           GS FAP S++ +YKRSFNGF  KLTE EA+K++ MEGV+SVF N   +LHTTRSWDF+GF
Sbjct: 61  GSTFAPESVLHTYKRSFNGFAVKLTEEEAEKIASMEGVVSVFLNEMNELHTTRSWDFLGF 120

Query: 124 SEQVKRIPAVESNIIVGVFDSGIWPESPSFDDTGYGPPPAKWRGRCEASANFSCNNKIIG 183
              V R   VESNI+VGV D+GIWPESPSFDD G+ PPP KW+G CE S NF CN KIIG
Sbjct: 121 PLTVPRRSQVESNIVVGVLDTGIWPESPSFDDEGFSPPPPKWKGTCETSNNFRCNRKIIG 180

Query: 184 ARSYRSNGKHLENDIKGPIDSDGHGTHTASTVAGGLVRQASMLGLGLGTARGGVPSARIA 243
           ARSY         D+ GP D++GHGTHTAST AGGLV QA++ GLGLGTARGGVP ARIA
Sbjct: 181 ARSYHIGRPISPGDVNGPRDTNGHGTHTASTAAGGLVSQANLYGLGLGTARGGVPLARIA 240

Query: 244 AYKVCWSDGCTDADILAAFDDAIADGVDIISGSLGGSSVKDYFNDSIAIGSFHAMKKGIL 303
           AYKVCW+DGC+D DILAA+DDAIADGVDIIS S+GG++ + YF D+IAIGSFHA+++GIL
Sbjct: 241 AYKVCWNDGCSDTDILAAYDDAIADGVDIISLSVGGANPRHYFVDAIAIGSFHAVERGIL 300

Query: 304 TTLAVGNNGPEFTTIVNFSPWSLSVAASTTDRKFETKVKLGDGREFNGVSVNTFDLNGTQ 363
           T+ + GN GP F T  + SPW LSVAAST DRKF T+V++G+G+ F GVS+NTFD     
Sbjct: 301 TSNSAGNGGPNFFTTASLSPWLLSVAASTMDRKFVTQVQIGNGQSFQGVSINTFD--NQY 360

Query: 364 IPLVYAGDIPKAPFDRSVSRMCFENTVDKERVKGKIVVCDSLAVPGGVV-AVEGAAGIIM 423
            PLV   DIP   FD+S SR C + +V+   +KGKIVVC++   P     +++GAAG++M
Sbjct: 361 YPLVSGRDIPNTGFDKSTSRFCTDKSVNPNLLKGKIVVCEASFGPHEFFKSLDGAAGVLM 420

Query: 424 QDESPQDDTNSYPLPAS---HNTLIITYMMIY--RVPTASIMKSRGRKRNRAPFVASFSS 483
              + +D  +SYPLP+S    N L+ T   IY  R P A+I KS       AP V SFSS
Sbjct: 421 TSNT-RDYADSYPLPSSVLDPNDLLATLRYIYSIRSPGATIFKSTTILNASAPVVVSFSS 480

Query: 484 RGPNPITPNILKPDISGPGVEILAAWPSTVSPSGAEEDNKRVVYNVISGTSMACPHVTAA 543
           RGPN  T +++KPDISGPGVEILAAWPS V+P G    N   ++N+ISGTSM+CPH+T  
Sbjct: 481 RGPNRATKDVIKPDISGPGVEILAAWPS-VAPVGGIRRN--TLFNIISGTSMSCPHITGI 540

Query: 544 AAYVKSFHPTWSPAALKSALITTAFPMSPKRNPDGELAYGAGHINPLGAVHPGLIYNASE 603
           A YVK+++PTWSPAA+KSAL+TTA PM+ + NP  E AYG+GH+NPL AV PGL+Y+A+E
Sbjct: 541 ATYVKTYNPTWSPAAIKSALMTTASPMNARFNPQAEFAYGSGHVNPLKAVRPGLVYDANE 600

Query: 604 TDYVKFLCGQGYSTESLRRLSDDSASCSANNSGTVFELNYPSFALSTNASNPISQVFRRR 663
           +DYVKFLCGQGY+T+++RR++ D ++C++ N+G V++LNYPSF LS + S   +Q F R 
Sbjct: 601 SDYVKFLCGQGYNTQAVRRITGDYSACTSGNTGRVWDLNYPSFGLSVSPSQTFNQYFNRT 660

Query: 664 VTNVGSSYATSYKAVIVNPWRDLKITVKPSVLLFKRLGEELRFEVRIGGKIRKGIESAAL 723
           +T+V +  A++Y+A+I  P + L I+V P+VL F  LG+   F + + G I+  + SA+L
Sbjct: 661 LTSV-APQASTYRAMISAP-QGLTISVNPNVLSFNGLGDRKSFTLTVRGSIKGFVVSASL 720

Query: 724 VWDDGKHKVRSPITV 728
           VW DG H VRSPIT+
Sbjct: 721 VWSDGVHYVRSPITI 727

BLAST of Cp4.1LG01g14250 vs. Swiss-Prot
Match: SBT43_ARATH (Subtilisin-like protease SBT4.3 OS=Arabidopsis thaliana GN=SBT4.3 PE=3 SV=1)

HSP 1 Score: 650.2 bits (1676), Expect = 2.7e-185
Identity = 356/741 (48.04%), Postives = 475/741 (64.10%), Query Frame = 1

Query: 1   MASLSTTLLFLSFCFSLLFFSSFSQEDPQKT---YIVYMGSHPKGKVSTSSHHIRFLTET 60
           MA LST L  +  C + +F    S  D ++    YIVYMG+ P+ K S  SHH+  L + 
Sbjct: 1   MAKLSTPLYLI--CLAFIFTRDVSANDYRQASSVYIVYMGTLPEIKYSPPSHHLSILQKL 60

Query: 61  IGSNFAPRSLIRSYKRSFNGFVAKLTEAEAKKVSEMEGVISVFPNGKKQLHTTRSWDFMG 120
           +G+  A   L+RSYKRSFNGF A L++AE++K+  M+ V+SVFP+   +L TTRSWDF+G
Sbjct: 61  VGTIAASHLLVRSYKRSFNGFAANLSQAESQKLQNMKEVVSVFPSKSHELTTTRSWDFVG 120

Query: 121 FSEQVKRIPAVESNIIVGVFDSGIWPESPSFDDTGYGPPPAKWRGRCEASANFSCNNKII 180
           F E+ +R    ES++IVGV DSGIWPES SFDD G+GPPP KW+G C+    F+CNNK+I
Sbjct: 121 FGEKARRESVKESDVIVGVIDSGIWPESESFDDEGFGPPPKKWKGSCKGGLKFACNNKLI 180

Query: 181 GARSYRSNGKHLENDIKGPIDSDGHGTHTASTVAGGLVRQASMLGLGLGTARGGVPSARI 240
           GAR Y               D +GHGTHTAST AG  V+ AS  GL  GTARGGVPSARI
Sbjct: 181 GARFYNKFADSAR-------DEEGHGTHTASTAAGNAVQAASFYGLAQGTARGGVPSARI 240

Query: 241 AAYKVCWSDGCTDADILAAFDDAIADGVDIISGSLGGSSVKDYFNDSIAIGSFHAMKKGI 300
           AAYKVC++  C D DILAAFDDAIADGVD+IS S+    V +  N S+AIGSFHAM +GI
Sbjct: 241 AAYKVCFNR-CNDVDILAAFDDAIADGVDVISISISADYVSNLLNASVAIGSFHAMMRGI 300

Query: 301 LTTLAVGNNGPEFTTIVNFSPWSLSVAASTTDRKFETKVKLGDGREFNGVSVNTFDLNGT 360
           +T  + GNNGP+  ++ N SPW ++VAAS TDR+F  +V LG+G+   G+SVNTF+LNGT
Sbjct: 301 ITAGSAGNNGPDQGSVANVSPWMITVAASGTDRQFIDRVVLGNGKALTGISVNTFNLNGT 360

Query: 361 QIPLVYAGDIPKAPFDRSVSRMCFENTVDKERVKGKIVVCDSLAVPGGVVAVEGAAGIIM 420
           + P+VY  ++ +    ++ +  C    VD E VKGKIV+CD   +      + GA G+I+
Sbjct: 361 KFPIVYGQNVSRN-CSQAQAGYCSSGCVDSELVKGKIVLCDDF-LGYREAYLAGAIGVIV 420

Query: 421 QDESPQDDTNSYPLPASHNTL-----IITYMMIYRVPTASIMKSRGRKRNRAPFVASFSS 480
           Q+    D     P PAS         I +Y+     P A I+++       AP+V SFSS
Sbjct: 421 QNTLLPDSAFVVPFPASSLGFEDYKSIKSYIESAEPPQAEILRTEEIVDREAPYVPSFSS 480

Query: 481 RGPNPITPNILKPDISGPGVEILAAWPSTVSPSGA--EEDNKRVVYNVISGTSMACPHVT 540
           RGP+ +  N+LKPD+S PG+EILAA+    SPS     ED + V Y+V+SGTSMACPHV 
Sbjct: 481 RGPSFVIQNLLKPDVSAPGLEILAAFSPVASPSSFLNPEDKRSVRYSVMSGTSMACPHVA 540

Query: 541 AAAAYVKSFHPTWSPAALKSALITTAFPMSPKRNPDGELAYGAGHINPLGAVHPGLIYNA 600
             AAYVKSFHP WSP+A+KSA++TTA PM+ K+NP+ E AYG+G INP  A  PGL+Y  
Sbjct: 541 GVAAYVKSFHPDWSPSAIKSAIMTTATPMNLKKNPEQEFAYGSGQINPTKASDPGLVYEV 600

Query: 601 SETDYVKFLCGQGYSTESLRRLSDDSASCSANNSGTVFELNYPSFALSTNASNPISQVFR 660
              DY+K LC +G+ + +L   S  + +CS      V +LNYP+     ++ +P +  F+
Sbjct: 601 ETEDYLKMLCAEGFDSTTLTTTSGQNVTCSERTE--VKDLNYPTMTTFVSSLDPFNVTFK 660

Query: 661 RRVTNVGSSYATSYKAVIVNPWRDLKITVKPSVLLFKRLGEELRFEVRIGGKIRK--GIE 720
           R VTNVG   +T YKA +V    +L+I+++P +L F  L E+  F V I GK  K     
Sbjct: 661 RTVTNVGFPNST-YKASVVPLQPELQISIEPEILRFGFLEEKKSFVVTISGKELKDGSFV 720

Query: 721 SAALVWDDGKHKVRSPITVFN 730
           S+++VW DG H VRSPI  ++
Sbjct: 721 SSSVVWSDGSHSVRSPIVAYS 726

BLAST of Cp4.1LG01g14250 vs. Swiss-Prot
Match: SBT46_ARATH (Subtilisin-like protease SBT4.6 OS=Arabidopsis thaliana GN=SBT4.6 PE=3 SV=1)

HSP 1 Score: 625.9 bits (1613), Expect = 5.4e-178
Identity = 352/741 (47.50%), Postives = 468/741 (63.16%), Query Frame = 1

Query: 3   SLSTTLLFLSFCFSLLFFSSFSQEDPQK-TYIVYMGSHP-KGKVSTSSHHIRFLTETIGS 62
           ++S  LL   F   ++ F+S  ++D  K  YIVYMG+ P +      SHH   L +  G 
Sbjct: 4   AVSYCLLSCIFALLVVSFASAGKDDQDKQVYIVYMGALPSRVDYMPMSHHTSILQDVTGE 63

Query: 63  NFAPRSLIRSYKRSFNGFVAKLTEAEAKKVSEMEGVISVFPNGKKQLHTTRSWDFMGFSE 122
           +     L+R+YKRSFNGF A+LTE+E + ++ M+ V+SVFP+    L TT SW+FMG  E
Sbjct: 64  SSIQDRLVRNYKRSFNGFAARLTESEREILASMDEVVSVFPSKNLNLQTTTSWNFMGLKE 123

Query: 123 --QVKRIPAVESNIIVGVFDSGIWPESPSFDDTGYGPPPAKWRGRCEASANFSCNNKIIG 182
             + KR P +ES+ I+GV DSGI+PES SF   G+GPPP KW+G C+   NF+CNNK+IG
Sbjct: 124 GKRTKRNPLIESDTIIGVIDSGIYPESDSFSGKGFGPPPKKWKGVCKGGTNFTCNNKLIG 183

Query: 183 ARSYRSNGKHLENDIKGPIDSDGHGTHTASTVAGGLVRQASMLGLGLGTARGGVPSARIA 242
           AR Y      LE   +   D+ GHG+HTAS  AG  V+  S  GLG GT RGGVP+ARIA
Sbjct: 184 ARYYTPK---LEGFPESARDNTGHGSHTASIAAGNAVKHVSFYGLGNGTVRGGVPAARIA 243

Query: 243 AYKVCWSD--GCTDADILAAFDDAIADGVDIISGSLGGSSVKDYFNDSIAIGSFHAMKKG 302
            YKVC      CT   ILAAFDDAIAD VDII+ SLG  +V  +  D++AIG+FHAM KG
Sbjct: 244 VYKVCDPGVIRCTSDGILAAFDDAIADKVDIITVSLGADAVGTFEEDTLAIGAFHAMAKG 303

Query: 303 ILTTLAVGNNGPEFTTIVNFSPWSLSVAASTTDRKFETKVKLGDGREFNGVSVNTFDLNG 362
           ILT    GNNGPE  TIV+ +PW  +VAAS  +R F TKV LG+G+   G SVN+FDLNG
Sbjct: 304 ILTVNGAGNNGPERRTIVSMAPWLFTVAASNMNRAFITKVVLGNGKTIVGRSVNSFDLNG 363

Query: 363 TQIPLVYAGDIPKAPFDRSVSRMCFENTVDKERVKGKIVVCDSLAVPGGVVAVEGAAGII 422
            + PLVY G    +  D S +  C    +D +RVKGKIV+CD+   PG   A+   A I+
Sbjct: 364 KKYPLVY-GKSASSRCDASSAGFCSPGCLDSKRVKGKIVLCDTQRNPGEAQAMGAVASIV 423

Query: 423 MQDESPQDDTNSYP---LPASHNTLIITYMMIYRVPTASIMKSRGRKRNRAPFVASFSSR 482
                      S+P   L      ++++Y+   + P A+++KS      +AP VAS+SSR
Sbjct: 424 RNPYEDAASVFSFPVSVLSEDDYNIVLSYVNSTKNPKAAVLKSETIFNQKAPVVASYSSR 483

Query: 483 GPNPITPNILKPDISGPGVEILAAWPSTVSPSGAEEDNKRVVYNVISGTSMACPHVTAAA 542
           GPNP+  +ILKPDI+ PG EILAA+   V PS  E D + V Y VISGTSM+CPHV   A
Sbjct: 484 GPNPLIHDILKPDITAPGSEILAAYSPYVPPS--ESDTRHVKYTVISGTSMSCPHVAGVA 543

Query: 543 AYVKSFHPTWSPAALKSALITTAFPMSPKRNPDGEL---AYGAGHINPLGAVHPGLIYNA 602
           AY+K+FHP WSP+ ++SA++TTA+PM+   +P  EL   AYGAGH++P+ A+HPGL+Y A
Sbjct: 544 AYIKTFHPLWSPSMIQSAIMTTAWPMNASTSPSNELAEFAYGAGHVDPIAAIHPGLVYEA 603

Query: 603 SETDYVKFLCGQGYSTESLRRLSDDSASCSANNSGTVF-ELNYPSFALSTNASNPISQVF 662
           +++D++ FLCG  Y+ + LR +S DS+SC+   + ++   LNYPS +   + + P    F
Sbjct: 604 NKSDHITFLCGFNYTGKKLRLISGDSSSCTKEQTKSLTRNLNYPSMSAQVSGTKPFKVTF 663

Query: 663 RRRVTNVGSSYATSYKAVIVNPWRDLKITVKPSVLLFKRLGEELRFEVRIGGKIRK--GI 722
           RR VTNVG   AT YKA +V     LK+ V P+VL  K L E+  F V + G   K   +
Sbjct: 664 RRTVTNVGRPNAT-YKAKVVG--SKLKVKVVPAVLSLKSLYEKKSFTVTVSGAGPKAENL 723

Query: 723 ESAALVWDDGKHKVRSPITVF 729
            SA L+W DG H VRSPI V+
Sbjct: 724 VSAQLIWSDGVHFVRSPIVVY 735

BLAST of Cp4.1LG01g14250 vs. Swiss-Prot
Match: SBT44_ARATH (Subtilisin-like protease SBT4.4 OS=Arabidopsis thaliana GN=SBT4.4 PE=2 SV=1)

HSP 1 Score: 620.9 bits (1600), Expect = 1.8e-176
Identity = 342/741 (46.15%), Postives = 470/741 (63.43%), Query Frame = 1

Query: 6   TTLLFLSFCFSLLFFSSFSQEDP----QKTYIVYMGSHPKGKVSTS-SHHIRFLTETIGS 65
           TT +FL     +L  SS S +      Q+ YIVY+GS P  +  T  S H+  L E  G 
Sbjct: 5   TTFIFLFSSLLVLSLSSVSADKDDHGDQQVYIVYLGSLPSREEYTPMSDHMSILQEITGE 64

Query: 66  NFAPRSLIRSYKRSFNGFVAKLTEAEAKKVSEMEGVISVFPNGKKQLHTTRSWDFMGFSE 125
           +     L+RSYK+SFNGF A+LTE+E K+++ ME V+SVFP+ K +L TT SW+FMG  E
Sbjct: 65  SLIENRLVRSYKKSFNGFAARLTESERKRLAGMERVVSVFPSRKLKLQTTSSWNFMGLKE 124

Query: 126 QVK--RIPAVESNIIVGVFDSGIWPESPSFDDTGYGPPPAKWRGRCEASANFSCNNKIIG 185
            +K  R  ++ES+ I+GV DSGI+PES SF D G+GPPP KW+G C    NF+CNNK+IG
Sbjct: 125 GIKTKRTRSIESDTIIGVIDSGIYPESDSFSDQGFGPPPKKWKGTCAGGKNFTCNNKVIG 184

Query: 186 ARSYRSNGKHLENDIKGPIDSDGHGTHTASTVAGGLVRQASMLGLGLGTARGGVPSARIA 245
           AR Y +  K  +       D  GHGTHTAS  AG  V  ++  GLG GTARGGVP+ARIA
Sbjct: 185 ARDYTAKSKANQT----ARDYSGHGTHTASIAAGNAVANSNFYGLGNGTARGGVPAARIA 244

Query: 246 AYKVCWSDGCTDADILAAFDDAIADGVDIISGSLGGSSVKDYFNDSIAIGSFHAMKKGIL 305
            YKVC ++GC    +++AFDDAIADGVD+IS S+   ++  +  D IAIG+FHAM  G+L
Sbjct: 245 VYKVCDNEGCDGEAMMSAFDDAIADGVDVISISIVLDNIPPFEEDPIAIGAFHAMAVGVL 304

Query: 306 TTLAVGNNGPEFTTIVNFSPWSLSVAASTTDRKFETKVKLGDGREFNGVSVNTFDLNGTQ 365
           T  A GNNGP+ +T+ + +PW  SVAAS T+R F  KV LGDG+   G SVNT+D+NGT 
Sbjct: 305 TVNAAGNNGPKISTVTSTAPWVFSVAASVTNRAFMAKVVLGDGKILIGRSVNTYDMNGTN 364

Query: 366 IPLVYAGDIPKAPFDRSVSRMCFENTVDKERVKGKIVVCDSLAVPGGVVAVE--GAAGII 425
            PLVY      +      +R+C    +D + VKGKIV+CDS     G++  +  GA G I
Sbjct: 365 YPLVYGKSAALSTCSVDKARLCEPKCLDGKLVKGKIVLCDSTK---GLIEAQKLGAVGSI 424

Query: 426 MQDESP-QDDTNSYPLPASHN---TLIITYMMIYRVPTASIMKSRGRKRNRAPFVASFSS 485
           +++  P +    S+P+    N     +++YM   + P A+++KS      RAP VASFSS
Sbjct: 425 VKNPEPDRAFIRSFPVSFLSNDDYKSLVSYMNSTKNPKATVLKSEEISNQRAPLVASFSS 484

Query: 486 RGPNPITPNILKPDISGPGVEILAAWPSTVSPSGAEEDNKRVVYNVISGTSMACPHVTAA 545
           RGP+ I  +ILKPDI+ PGVEILAA+    SP+ +E D +RV Y+V+SGTSMACPHV   
Sbjct: 485 RGPSSIVSDILKPDITAPGVEILAAYSPDSSPTESEFDTRRVKYSVLSGTSMACPHVAGV 544

Query: 546 AAYVKSFHPTWSPAALKSALITTAFPMSPKRN--PDGELAYGAGHINPLGAVHPGLIYNA 605
           AAYVK+FHP WSP+ ++SA++TTA+PM+   +     E AYG+GH++P+ A++PGL+Y  
Sbjct: 545 AAYVKTFHPQWSPSMIQSAIMTTAWPMNASGSGFVSTEFAYGSGHVDPIDAINPGLVYEL 604

Query: 606 SETDYVKFLCGQGYSTESLRRLSDDSASCSANNSGTV-FELNYPSFALSTNASNPISQVF 665
           ++ D++ FLCG  Y+++ LR +S D+++C+   S T+   LNYP+ +   + + P +  F
Sbjct: 605 TKADHINFLCGLNYTSDHLRIISGDNSTCTKEISKTLPRNLNYPTMSAKVSGTKPFNITF 664

Query: 666 RRRVTNVGSSYATSYKAVIVNPWRDLKITVKPSVLLFKRLGEELRFEVRIGGKI--RKGI 725
           +R VTNVG   +T    V+  P   L I V P VL  K + E+  F V +       K  
Sbjct: 665 QRTVTNVGMQKSTYNAKVVKFPGSKLSIKVSPRVLSMKSMNEKQSFMVTVSSDSIGTKQP 724

Query: 726 ESAALVWDDGKHKVRSPITVF 729
            SA L+W DG H VRSPI V+
Sbjct: 725 VSANLIWSDGTHNVRSPIIVY 738

BLAST of Cp4.1LG01g14250 vs. Swiss-Prot
Match: SBT4C_ARATH (Subtilisin-like protease SBT4.12 OS=Arabidopsis thaliana GN=SBT4.12 PE=2 SV=1)

HSP 1 Score: 619.0 bits (1595), Expect = 6.7e-176
Identity = 344/744 (46.24%), Postives = 462/744 (62.10%), Query Frame = 1

Query: 1   MASLSTTLLFLSFCFSLLF--FSSFSQEDPQKTYIVYMGS-HPKGKVSTSSHHIRFLTET 60
           MA+L+ +    S+   LL    S+   ED Q  YIVYMGS   +     +S H+  L + 
Sbjct: 1   MANLAASTCLYSWLLVLLLSSVSAIIDEDTQ-VYIVYMGSLSSRADYIPTSDHMSILQQV 60

Query: 61  IGSNFAPRSLIRSYKRSFNGFVAKLTEAEAKKVSEMEGVISVFPNGKKQLHTTRSWDFMG 120
            G +     L+RSYKRSFNGF A+LTE+E   ++E+EGV+SVFPN   QLHTT SWDFMG
Sbjct: 61  TGESSIEGRLVRSYKRSFNGFAARLTESERTLIAEIEGVVSVFPNKILQLHTTTSWDFMG 120

Query: 121 FSE--QVKRIPAVESNIIVGVFDSGIWPESPSFDDTGYGPPPAKWRGRCEASANFSCNNK 180
             E    KR  A+ES+ I+GV D+GIWPES SF D G+GPPP KW+G C    NF+CNNK
Sbjct: 121 VKEGKNTKRNLAIESDTIIGVIDTGIWPESKSFSDKGFGPPPKKWKGVCSGGKNFTCNNK 180

Query: 181 IIGARSYRSNGKHLENDIKGPIDSDGHGTHTASTVAGGLVRQASMLGLGLGTARGGVPSA 240
           +IGAR Y S G           D+ GHGTHTAST AG  V+  S  G+G GT RGGVP++
Sbjct: 181 LIGARDYTSEGTR---------DTSGHGTHTASTAAGNAVKDTSFFGIGNGTVRGGVPAS 240

Query: 241 RIAAYKVCWSDGCTDADILAAFDDAIADGVDIISGSLGGSSVKDYFNDSIAIGSFHAMKK 300
           RIAAYKVC   GC+   +L++FDDAIADGVD+I+ S+G      + +D IAIG+FHAM K
Sbjct: 241 RIAAYKVCTDSGCSSEALLSSFDDAIADGVDLITISIGFQFPSIFEDDPIAIGAFHAMAK 300

Query: 301 GILTTLAVGNNGPEFTTIVNFSPWSLSVAASTTDRKFETKVKLGDGREFNGVSVNTFDLN 360
           GILT  + GN+GP+ TT+ + +PW  +VAASTT+R F TKV LG+G+   G SVN FD+ 
Sbjct: 301 GILTVSSAGNSGPKPTTVSHVAPWIFTVAASTTNRGFITKVVLGNGKTLAGRSVNAFDMK 360

Query: 361 GTQIPLVYAGDIPKAPFDRSVSRMCFENTVDKERVKGKIVVCDSLAVPGGVVAVEGAAGI 420
           G + PLVY      +  D   + +C    ++K RVKGKI+VC     P G    +    I
Sbjct: 361 GKKYPLVYGKSAASSACDAKTAALCAPACLNKSRVKGKILVCGG---PSGYKIAKSVGAI 420

Query: 421 IMQDESPQDD---TNSYP---LPASHNTLIITYMMIYRVPTASIMKSRGRKRNRAPFVAS 480
            + D+SP+ D   T+  P   L A     +++Y+     P A+++K+       +P +AS
Sbjct: 421 AIIDKSPRPDVAFTHHLPASGLKAKDFKSLVSYIESQDSPQAAVLKTETIFNRTSPVIAS 480

Query: 481 FSSRGPNPITPNILKPDISGPGVEILAAWPSTVSPSGAEEDNKRVVYNVISGTSMACPHV 540
           FSSRGPN I  +ILKPDI+ PGVEILAA+     PS  E+D +RV Y+V SGTSMACPHV
Sbjct: 481 FSSRGPNTIAVDILKPDITAPGVEILAAFSPNGEPS--EDDTRRVKYSVFSGTSMACPHV 540

Query: 541 TAAAAYVKSFHPTWSPAALKSALITTAFPMSPKRN--PDGELAYGAGHINPLGAVHPGLI 600
              AAYVK+F+P WSP+ ++SA++TTA+P+  K       E AYGAGH++P+ A++PGL+
Sbjct: 541 AGVAAYVKTFYPRWSPSMIQSAIMTTAWPVKAKGRGIASTEFAYGAGHVDPMAALNPGLV 600

Query: 601 YNASETDYVKFLCGQGYSTESLRRLSDDSASCSANNSGTVFELNYPSF-ALSTNASNPIS 660
           Y   + D++ FLCG  Y++++L+ +S D+  CS  N      LNYPS  A  +   +  S
Sbjct: 601 YELDKADHIAFLCGMNYTSKTLKIISGDTVKCSKKNKILPRNLNYPSMSAKLSGTDSTFS 660

Query: 661 QVFRRRVTNVGSSYATSYKAVIVNPWRDLKITVKPSVLLFKRLGEELRFEVRI-GGKIRK 720
             F R +TNVG+  +T    V+      L I V PSVL FK + E+  F V + G  +  
Sbjct: 661 VTFNRTLTNVGTPNSTYKSKVVAGHGSKLSIKVTPSVLYFKTVNEKQSFSVTVTGSDVDS 720

Query: 721 GIESAA-LVWDDGKHKVRSPITVF 729
            + S+A L+W DG H VRSPI V+
Sbjct: 721 EVPSSANLIWSDGTHNVRSPIVVY 729

BLAST of Cp4.1LG01g14250 vs. TrEMBL
Match: A0A0A0KWL9_CUCSA (Uncharacterized protein OS=Cucumis sativus GN=Csa_5G613590 PE=4 SV=1)

HSP 1 Score: 1193.3 bits (3086), Expect = 0.0e+00
Identity = 600/740 (81.08%), Postives = 655/740 (88.51%), Query Frame = 1

Query: 1   MASLSTTLLFLSFCFSLLFFSSFSQE--DPQKTYIVYMGSHPKGKVSTSSHHIRFLTETI 60
           M+SL   LLFLSFC  LLFF S SQ+  D QKTYIVYMGSH KGKVSTSSHHIR L ETI
Sbjct: 1   MSSLIPRLLFLSFCLFLLFFCSNSQDNYDSQKTYIVYMGSHSKGKVSTSSHHIRLLKETI 60

Query: 61  GSNFAPRSLIRSYKRSFNGFVAKLTEAEAKKVSEMEGVISVFPNGKKQLHTTRSWDFMGF 120
           GS+F P SL+ S+KRSFNGFVAKLTEAE KKVSEMEGVISVFPNGKKQLHTTRSWDFMGF
Sbjct: 61  GSSFPPHSLLHSFKRSFNGFVAKLTEAEVKKVSEMEGVISVFPNGKKQLHTTRSWDFMGF 120

Query: 121 SEQVKRIPAVESNIIVGVFDSGIWPESPSFDDTGYGPPPAKWRGRCEASANFSCNNKIIG 180
           SEQVKR+PAVESN+IVGV DSGIWPESPSFD  GYG PPAKW+G CE SANFSCNNKIIG
Sbjct: 121 SEQVKRVPAVESNVIVGVLDSGIWPESPSFDHAGYGSPPAKWKGSCEVSANFSCNNKIIG 180

Query: 181 ARSYRSNGKHLENDIKGPIDSDGHGTHTASTVAGGLVRQASMLGLGLGTARGGVPSARIA 240
           ARSYRSNG++ E DIKGP DSDGHGTHTAS VAGGLVR+ASMLGLGLGTARGGVPSARIA
Sbjct: 181 ARSYRSNGEYPEGDIKGPRDSDGHGTHTASIVAGGLVRRASMLGLGLGTARGGVPSARIA 240

Query: 241 AYKVCWSDGCTDADILAAFDDAIADGVDIISGSLGGSSVKDYFNDSIAIGSFHAMKKGIL 300
           AYKVCWSDGC+DADILAAFDDAIADGVDIISGSLGGS  +DYFNDSIAIGSFHAMKKGIL
Sbjct: 241 AYKVCWSDGCSDADILAAFDDAIADGVDIISGSLGGSGARDYFNDSIAIGSFHAMKKGIL 300

Query: 301 TTLAVGNNGPEFTTIVNFSPWSLSVAASTTDRKFETKVKLGDGREFNGVSVNTFDLNGTQ 360
           T+LAVGNNGP+FTTIVNFSPWSLSVAASTTDRKFETKV+LGDGREF+GVSVNTFD+ G Q
Sbjct: 301 TSLAVGNNGPDFTTIVNFSPWSLSVAASTTDRKFETKVELGDGREFSGVSVNTFDIKGKQ 360

Query: 361 IPLVYAGDIPKAPFDRSVSRMCFENTVDKERVKGKIVVCDSLAVPGGVVAVEGAAGIIMQ 420
           IPLVYAGDIPKAPFD SVSR+CFENTVD + VKGKIVVCDSL VPGGVVAV+GA GIIMQ
Sbjct: 361 IPLVYAGDIPKAPFDSSVSRLCFENTVDLKLVKGKIVVCDSLTVPGGVVAVKGAVGIIMQ 420

Query: 421 DESPQDDTNSYPLPASH-----NTLIITYM-MIYRVPTASIMKSRGRKRNRAPFVASFSS 480
           D+S  DDTNS+P+PASH       L+++Y+     +PTA+I KS  RKR RAP VASFSS
Sbjct: 421 DDSSHDDTNSFPIPASHLGPKAGALVLSYINSTNSIPTATIKKSTERKRKRAPSVASFSS 480

Query: 481 RGPNPITPNILKPDISGPGVEILAAWPSTVSPSGAEEDNKRVVYNVISGTSMACPHVTAA 540
           RGPNPITPNILKPD+SGPGVEILAAW     PSGAEEDNKRV+YN+ISGTSMACPHVTAA
Sbjct: 481 RGPNPITPNILKPDLSGPGVEILAAWSPVSPPSGAEEDNKRVLYNIISGTSMACPHVTAA 540

Query: 541 AAYVKSFHPTWSPAALKSALITTAFPMSPKRNPDGELAYGAGHINPLGAVHPGLIYNASE 600
           AAYVKSFHPTWSP+ALKSALITTAFPMSPK NPD E  YGAGHINPLGAVHPGLIY+ASE
Sbjct: 541 AAYVKSFHPTWSPSALKSALITTAFPMSPKHNPDKEFGYGAGHINPLGAVHPGLIYDASE 600

Query: 601 TDYVKFLCGQGYSTESLRRLSDDSASCSANNSGTVFELNYPSFALSTNASNPISQVFRRR 660
            DYV+FLCGQGY+TE L+ +S+D+ +CS+NNS TVF+LNYPSFALSTN S PI+QV++R 
Sbjct: 601 IDYVQFLCGQGYTTELLQLVSEDNNTCSSNNSDTVFDLNYPSFALSTNISKPINQVYKRT 660

Query: 661 VTNVGSSYATSYKAVIVNPWRDLKITVKPSVLLFKRLGEELRFEVRIGGKIRKGIESAAL 720
           VTNVGS YAT YKA ++NPW++L+I V PSVL FK LGE+  FEV I GKIRK IESA+L
Sbjct: 661 VTNVGSKYAT-YKATVINPWKNLEIKVNPSVLSFKNLGEKQSFEVTIRGKIRKDIESASL 720

Query: 721 VWDDGKHKVRSPITVFNANL 733
           VWDDGKHKVRSPITVF AN+
Sbjct: 721 VWDDGKHKVRSPITVFIANI 739

BLAST of Cp4.1LG01g14250 vs. TrEMBL
Match: A0A0A0KRC4_CUCSA (Uncharacterized protein OS=Cucumis sativus GN=Csa_5G613580 PE=4 SV=1)

HSP 1 Score: 1085.1 bits (2805), Expect = 0.0e+00
Identity = 543/740 (73.38%), Postives = 624/740 (84.32%), Query Frame = 1

Query: 1   MASLSTTLLFLSFCFSLLFFSSFSQED-PQKTYIVYMGSHPKGKVSTSSHHIRFLTETIG 60
           M+ L + LLFL+FCF LLFFS+ SQ++  QKTYIVYMG+HPKGK STSSHH+R L E+IG
Sbjct: 1   MSPLISKLLFLNFCFFLLFFSTNSQDNGSQKTYIVYMGNHPKGKPSTSSHHMRLLKESIG 60

Query: 61  SNFAPRSLIRSYKRSFNGFVAKLTEAEAKKVSEMEGVISVFPNGKKQLHTTRSWDFMGFS 120
           S+F P SL+ SYKRSFNGFVAK+TE EAKKVSEMEGVISVFPNGKKQLHTTRSW+FMGFS
Sbjct: 61  SSFPPNSLLHSYKRSFNGFVAKMTEDEAKKVSEMEGVISVFPNGKKQLHTTRSWNFMGFS 120

Query: 121 EQVKRIPAVESNIIVGVFDSGIWPESPSFDDTGYGPPPAKWRGRCEASANFSCNNKIIGA 180
           EQVKR+P VES+IIVGVFD+GIWPESPSFDDTGYGPPPAKW+G CE SANFSCNNKIIGA
Sbjct: 121 EQVKRVPMVESDIIVGVFDTGIWPESPSFDDTGYGPPPAKWKGSCEVSANFSCNNKIIGA 180

Query: 181 RSYRSNGKHLENDIKGPIDSDGHGTHTASTVAGGLVRQASMLGLGLGTARGGVPSARIAA 240
           RSY S+G H E D++GPIDS+GHGTHTASTVAGGLVRQA+MLGLGLGTARGGVPSARIA 
Sbjct: 181 RSYHSSGPHPEGDLEGPIDSNGHGTHTASTVAGGLVRQANMLGLGLGTARGGVPSARIAV 240

Query: 241 YKVCWSDGCTDADILAAFDDAIADGVDIISGSLGGSSVKDYFNDSIAIGSFHAMKKGILT 300
           YK+CWSD C+DADILAAFDDAIADGVDI+S S+ G   K+YFNDS+AIGSFHAMKKGIL+
Sbjct: 241 YKICWSDNCSDADILAAFDDAIADGVDILSVSVAGPGFKNYFNDSMAIGSFHAMKKGILS 300

Query: 301 TLAVGNNGPEFTTIVNFSPWSLSVAASTTDRKFETKVKLGDGREFNGVSVNTFDLNGTQI 360
           + A GN GP   ++ N+SPWSL+VAASTTDR  ET V+LGDGRE  GV++NTFD+ G Q+
Sbjct: 301 SFAAGNTGPGSASVANYSPWSLTVAASTTDRVLETVVELGDGRELKGVTINTFDMKGKQV 360

Query: 361 PLVYAGDIPKAPFDRSVSRMCFENTVDKERVKGKIVVCDSLAV-PGGVVAVEGAAGIIMQ 420
           PLVY GDIPKA    S S  C  N+VD +  KGKIV+CD +   P   VAV+GA GIIMQ
Sbjct: 361 PLVYGGDIPKANTSSSFSSQCLRNSVDLKLAKGKIVMCDMITTSPAEAVAVKGAVGIIMQ 420

Query: 421 DESPQDDTNSYPLPASH-----NTLIITYM-MIYRVPTASIMKSRGRKRNRAPFVASFSS 480
           ++SP+D T S+P+PASH       LI++Y+     +PTA+I KS  RKR RAP VASFSS
Sbjct: 421 NDSPKDRTFSFPIPASHIDTKSGALILSYINSTNSIPTATIKKSIERKRRRAPSVASFSS 480

Query: 481 RGPNPITPNILKPDISGPGVEILAAWPSTVSPSGAEEDNKRVVYNVISGTSMACPHVTAA 540
           RGPNP+TPNILKPD+SGPGVEILAAWP   SPSGA EDNKRV+YN+ISGTSMACPHVTA 
Sbjct: 481 RGPNPVTPNILKPDLSGPGVEILAAWPPIASPSGAVEDNKRVLYNIISGTSMACPHVTAV 540

Query: 541 AAYVKSFHPTWSPAALKSALITTAFPMSPKRNPDGELAYGAGHINPLGAVHPGLIYNASE 600
           AAYVKSFHPTWSPAALKSAL+TTAFPMSPKRN D E AYGAGH+NPLGAVHPGLIY+ASE
Sbjct: 541 AAYVKSFHPTWSPAALKSALMTTAFPMSPKRNQDKEFAYGAGHLNPLGAVHPGLIYDASE 600

Query: 601 TDYVKFLCGQGYSTESLRRLSDDSASCSANNSGTVFELNYPSFALSTNASNPISQVFRRR 660
            DYV+FLCGQGY+TE L+ +SD S +CS+N+S TVF+LNYPSFALSTN S PI+QV+RR 
Sbjct: 601 IDYVRFLCGQGYTTELLQLVSDGSNTCSSNDSDTVFDLNYPSFALSTNISVPINQVYRRT 660

Query: 661 VTNVGSSYATSYKAVIVNPWRDLKITVKPSVLLFKRLGEELRFEVRIGGKIRKGIESAAL 720
           VTN+GS  A  YKA I+NPW++L I V PSVL F  LGE+  FEV I GKIR+ IESA+L
Sbjct: 661 VTNIGSRSA-MYKATIINPWKNLDIKVNPSVLSFTSLGEKQSFEVTIRGKIRRNIESASL 720

Query: 721 VWDDGKHKVRSPITVFNANL 733
           VW+DGKHKVRSPITVF+A +
Sbjct: 721 VWNDGKHKVRSPITVFDATI 739

BLAST of Cp4.1LG01g14250 vs. TrEMBL
Match: A0A0A0KJN5_CUCSA (Uncharacterized protein OS=Cucumis sativus GN=Csa_5G141040 PE=4 SV=1)

HSP 1 Score: 971.8 bits (2511), Expect = 4.5e-280
Identity = 502/742 (67.65%), Postives = 593/742 (79.92%), Query Frame = 1

Query: 1   MASLSTTLLFLSFCFSLLFFSSFSQEDP-QKTYIVYMGSHPKGKVS--TSSHHIRFLTET 60
           M+SLS  LLFL  CFSLLF +S S++D  +KTYIVYMGSH +   +  +S HH+R L E 
Sbjct: 1   MSSLSR-LLFLCLCFSLLFSTSVSEDDQYRKTYIVYMGSHHQVSSAPLSSHHHMRILQEA 60

Query: 61  IGSNFAPRSLIRSYKRSFNGFVAKLTEAEAKKVSEMEGVISVFPNGKKQLHTTRSWDFMG 120
           +GS FAP  L+ SYKRSFNGFVAKLTE EAKKVSEMEGVISVFPNG+ QLHTTRSWDFMG
Sbjct: 61  VGSTFAPHCLLHSYKRSFNGFVAKLTEIEAKKVSEMEGVISVFPNGELQLHTTRSWDFMG 120

Query: 121 FSEQVKRIPAVESNIIVGVFDSGIWPESPSFDDTGYGPPPAKWRGRCEASANFSCNNKII 180
            SEQV+R+P+VES+IIVGVFD+GIWPESPSF D GYGPPP KW+G CE SANFSCNNKII
Sbjct: 121 MSEQVERVPSVESDIIVGVFDTGIWPESPSFLDHGYGPPPPKWKGSCEVSANFSCNNKII 180

Query: 181 GARSYRSNGKHLENDIKGPIDSDGHGTHTASTVAGGLVRQASMLGLGLGTARGGVPSARI 240
           GARSYRS+G++  +DIKGP DS+GHGTH ASTVAGGLVRQASMLGLG+GTARGGVPSARI
Sbjct: 181 GARSYRSDGRYPIDDIKGPRDSNGHGTHAASTVAGGLVRQASMLGLGMGTARGGVPSARI 240

Query: 241 AAYKVCWSDGCTDADILAAFDDAIADGVDIISGSLGGSSVK-DYFNDSIAIGSFHAMKKG 300
           AAYKVCWSD C+DAD+LAAFDDAIADGVDIIS S+G    + +YF D IAIG+FHAM+ G
Sbjct: 241 AAYKVCWSDTCSDADVLAAFDDAIADGVDIISMSVGPKRPRPNYFQDPIAIGTFHAMRNG 300

Query: 301 ILTTLAVGNNGPEFTTIVNFSPWSLSVAASTTDRKFETKVKLGDGREFNGVSVNTFDLNG 360
           ILT+ + GN GP   T+ NFSPW+LSVAAST+DR+F T V+LGDGR+FNGV++NTFDLNG
Sbjct: 301 ILTSTSAGNEGPLHFTVTNFSPWALSVAASTSDRRFLTAVQLGDGRKFNGVTINTFDLNG 360

Query: 361 TQIPLVYAGDIPKAP--FDRSVSRMCFENTVDKERVKGKIVVCDSLAVPGGVVAVEGAAG 420
           TQ PLVYAG+IP     F+ S SR C  ++VD+E VKGKI +CDS   P  V ++E A G
Sbjct: 361 TQYPLVYAGNIPNVTGGFNGSFSRFCLRDSVDRELVKGKIAICDSFVSPSDVGSLESAVG 420

Query: 421 IIMQDESPQDDTNSYPLPASH-----NTLIITYMMIYRVPTASIMKSRGRKRNRAPFVAS 480
           IIMQD SP+D T ++PLPASH       LI +Y+   R+PTA+I+KS G K   AP VAS
Sbjct: 421 IIMQDRSPKDLTFAFPLPASHLGIQQRPLISSYLNSTRIPTATILKSTGLKLQVAPLVAS 480

Query: 481 FSSRGPNPITPNILKPDISGPGVEILAAWPSTVSPSGAEEDNKRVVYNVISGTSMACPHV 540
           FSSRGPNP +P ILKPD+ GPGVEILAAW    SPS A+ DN+++++N+ISGTSMACPH 
Sbjct: 481 FSSRGPNPTSPYILKPDVIGPGVEILAAWSPLRSPSNAKGDNRKLLFNIISGTSMACPHA 540

Query: 541 TAAAAYVKSFHPTWSPAALKSALITTAFPMSPKRNPDGELAYGAGHINPLGAVHPGLIYN 600
           TA AAYVKSFHP+WSPAALKSALITTAFPM     P+ E AYG+GHINPLGAV+PGLIYN
Sbjct: 541 TAVAAYVKSFHPSWSPAALKSALITTAFPMRGDLYPEAEFAYGSGHINPLGAVNPGLIYN 600

Query: 601 ASETDYVKFLCGQGYSTESLRRLSDDSASCSANNSGTVFELNYPSFALSTNASNPISQVF 660
           ASETDY++FLC +GY+T  LR ++ D+++CS   S  V++LNYPSFAL T+ S P SQ  
Sbjct: 601 ASETDYIRFLCDEGYNTTFLRIITKDNSTCSTTQSIRVYDLNYPSFALFTHISTPFSQTS 660

Query: 661 RRRVTNVGSSYATSYKAVIVNPWRDLKITVKPSVLLFKRLGEELRFEVRIGGKIRKGIES 720
           +RRVTNVGS+ +T YKA I  P   L ITV PS+L FK L EEL FEV   GKI + IES
Sbjct: 661 KRRVTNVGSTNST-YKATISAP-SGLNITVNPSILSFKALEEELNFEVTFEGKIDRSIES 720

Query: 721 AALVWDDGKHKVRSPITVFNAN 732
           A+LVWDDG HKVRSPI VF+++
Sbjct: 721 ASLVWDDGVHKVRSPIIVFDSD 739

BLAST of Cp4.1LG01g14250 vs. TrEMBL
Match: A0A0A0KLR4_CUCSA (Uncharacterized protein OS=Cucumis sativus GN=Csa_5G187880 PE=4 SV=1)

HSP 1 Score: 864.4 bits (2232), Expect = 1.0e-247
Identity = 435/700 (62.14%), Postives = 525/700 (75.00%), Query Frame = 1

Query: 36  MGSHPKGKVSTSSHHIRFLTETIGSNFAPRSLIRSYKRSFNGFVAKLTEAEAKKVSEMEG 95
           MG+  +   ST SHH+R L E +GS+FAP +L+ SYKRSFNGFV KLTE EA+K+S  E 
Sbjct: 1   MGNKLEDSASTPSHHMRMLEEVVGSSFAPEALLHSYKRSFNGFVVKLTEEEAQKISAKEN 60

Query: 96  VISVFPNGKKQLHTTRSWDFMGFSEQVKRIPAVESNIIVGVFDSGIWPESPSFDDTGYGP 155
           V+SVFPN KK LHTTRSWDFMGF+++  R+  VESNI+VGV DSGIWPESPSF D GYGP
Sbjct: 61  VVSVFPNEKKHLHTTRSWDFMGFTQKAPRVKQVESNIVVGVLDSGIWPESPSFSDVGYGP 120

Query: 156 PPAKWRGRCEASANFSCNNKIIGARSYRSNGKHLENDIKGPIDSDGHGTHTASTVAGGLV 215
           PP KW+G C+ SANF CN KIIGAR+YRS+      DIK P DSDGHGTHTASTVAGGLV
Sbjct: 121 PPPKWKGACQTSANFHCNRKIIGARAYRSDKFFPPEDIKSPRDSDGHGTHTASTVAGGLV 180

Query: 216 RQASMLGLGLGTARGGVPSARIAAYKVCWSDGCTDADILAAFDDAIADGVDIISGSLGGS 275
            QAS+ GL LGTARGGVPSARIA YK+CWSDGC DADILAAFDDAIADGVDIIS S+GGS
Sbjct: 181 NQASLYGLALGTARGGVPSARIAVYKICWSDGCYDADILAAFDDAIADGVDIISLSVGGS 240

Query: 276 SVKDYFNDSIAIGSFHAMKKGILTTLAVGNNGPEFTTIVNFSPWSLSVAASTTDRKFETK 335
             K YFNDSIAIG+FH+MK GILT+ + GN+GP++ TI NFSPWSLSVAAS+ DRK  ++
Sbjct: 241 KPKYYFNDSIAIGAFHSMKHGILTSNSAGNDGPDYFTIRNFSPWSLSVAASSIDRKLVSR 300

Query: 336 VKLGDGREFNGVSVNTFDLNGTQIPLVYAGDIP--KAPFDRSVSRMCFENTVDKERVKGK 395
           V+LG+   F G ++NTFDL G Q PL+YAG  P   A F  S SR C  N+VD+  VKGK
Sbjct: 301 VQLGNKNTFQGYTINTFDLKGKQHPLIYAGSAPNISAGFTGSSSRFCSRNSVDRNLVKGK 360

Query: 396 IVVCDSLAVPGGVVAVEGAAGIIMQDESPQDDTNSYPLPASH-----NTLIITYMMIYRV 455
           IV+CDS+  P   V++ GA G++M D   +D+  SYPLP+S+        I TYM   R 
Sbjct: 361 IVLCDSVLSPATFVSLNGAVGVVMNDLGVKDNARSYPLPSSYLDPVDGDNIKTYMDRTRF 420

Query: 456 PTASIMKSRGRKRNRAPFVASFSSRGPNPITPNILKPDISGPGVEILAAWPSTVSPSGAE 515
           PTA+I+KS       AP++ SFSSRGPNP T +ILKPD++ PGVEILAAW    + S   
Sbjct: 421 PTATILKSNAVNDTSAPWIVSFSSRGPNPETYDILKPDLTAPGVEILAAWSPIATVSSGV 480

Query: 516 EDNKRVVYNVISGTSMACPHVTAAAAYVKSFHPTWSPAALKSALITTAFPMSPKRNPDGE 575
            D++  +YN+ISGTSM+CPH TAAA YVK+FHPTWSPAA+KSAL+TTA P++ K N   E
Sbjct: 481 RDSRTTLYNIISGTSMSCPHATAAAVYVKTFHPTWSPAAIKSALMTTATPLNAKLNTQVE 540

Query: 576 LAYGAGHINPLGAVHPGLIYNASETDYVKFLCGQGYSTESLRRLSDDSASCSANNSGTVF 635
            AYGAGHINPL AVHPGL+Y+A E+DYV+FLCGQGY+T  +RRLS D++ C+  NSG V+
Sbjct: 541 FAYGAGHINPLRAVHPGLLYDAYESDYVRFLCGQGYTTAMVRRLSGDNSVCTRANSGRVW 600

Query: 636 ELNYPSFALSTNASNPISQVFRRRVTNVGSSYATSYKAVIVNPWRDLKITVKPSVLLFKR 695
           +LNYPSFALS+ +S   +Q FRR VTNVGS  +T Y+A +V   R L ITV P VL F  
Sbjct: 601 DLNYPSFALSSTSSQSFNQFFRRTVTNVGSKVST-YRAKVVGVPRGLSITVNPPVLSFNA 660

Query: 696 LGEELRFEVRIGGKIRKGIESAALVWDDGKHKVRSPITVF 729
           +G++  F + I G I + I SA+LVW DG H VRSPITVF
Sbjct: 661 IGQKKSFTLTIRGSISQSIVSASLVWSDGHHNVRSPITVF 699

BLAST of Cp4.1LG01g14250 vs. TrEMBL
Match: A0A0A0KLY4_CUCSA (Uncharacterized protein OS=Cucumis sativus GN=Csa_5G187860 PE=4 SV=1)

HSP 1 Score: 839.0 bits (2166), Expect = 4.6e-240
Identity = 428/729 (58.71%), Postives = 533/729 (73.11%), Query Frame = 1

Query: 8   LLFLSFCFSLLFFSSFSQEDPQKTYIVYMGSHPKGKVSTSSHHIRFLTETIGSNFAPRSL 67
           +L L    SLL     S+ D +K YIVYMG+ P+   ST SHH+R L E  GSNFAP SL
Sbjct: 12  VLVLGLVSSLLASGFDSKNDDRKIYIVYMGNKPQDTASTPSHHMRMLREVTGSNFAPESL 71

Query: 68  IRSYKRSFNGFVAKLTEAEAKKVSEMEGVISVFPNGKKQLHTTRSWDFMGFSEQVKRIPA 127
           + SYKRSFNGFV KLTE EA ++S  EGV+SVFP+GKK LHTTRSWDF+GF++ V R+  
Sbjct: 72  LHSYKRSFNGFVVKLTEEEAHRISAKEGVVSVFPSGKKHLHTTRSWDFIGFTKDVPRVNQ 131

Query: 128 VESNIIVGVFDSGIWPESPSFDDTGYGPPPAKWRGRCEASANFSCNNKIIGARSYRSNGK 187
           VES+I+VGV DSGIWPE+PSF D GYGP PAKW+G C+   NF+CN KIIGAR+YRS+  
Sbjct: 132 VESDIVVGVLDSGIWPENPSFSDAGYGPIPAKWKGICQNPTNFTCNKKIIGARAYRSDNV 191

Query: 188 HLENDIKGPIDSDGHGTHTASTVAGGLVRQASMLGLGLGTARGGVPSARIAAYKVCWSDG 247
               DI  P DS+GHGTHTASTVAGGLV QAS+ GL LGTARGGVPSARIA YK+CWSDG
Sbjct: 192 FPTEDIPSPRDSNGHGTHTASTVAGGLVSQASLYGLALGTARGGVPSARIAVYKICWSDG 251

Query: 248 CTDADILAAFDDAIADGVDIISGSLGGSSVKDYFNDSIAIGSFHAMKKGILTTLAVGNNG 307
           C+DADILAAFDDAIADGVDIIS S+GGS  + YFNDSIAIG+FH+MK GILT+ + GN+G
Sbjct: 252 CSDADILAAFDDAIADGVDIISLSVGGSEARYYFNDSIAIGAFHSMKHGILTSNSAGNDG 311

Query: 308 PEFTTIVNFSPWSLSVAASTTDRKFETKVKLGDGREFNGVSVNTFDLNGTQIPLVYAGDI 367
           P++ TI NFSPWSLSVAASTTDRK  ++V++G+   + G ++NTFD  G Q PL+YAGD 
Sbjct: 312 PDYFTIRNFSPWSLSVAASTTDRKLVSRVEIGNTNVYQGYTINTFDPLGKQYPLIYAGDA 371

Query: 368 PK--APFDRSVSRMCFENTVDKERVKGKIVVCDSLAVPGGVVAVEGAAGIIMQDESPQDD 427
           P     F  S+SR C E +VD   V GKI++CDS+  P   V    A G++M D+  +  
Sbjct: 372 PNLIGGFTGSISRFCSEGSVDANLVSGKILLCDSILAPSAFVYFSDAVGVVMNDDGVKYP 431

Query: 428 TNSYPLPASH-----NTLIITYMMIYRVPTASIMKSRGRKRNRAPFVASFSSRGPNPITP 487
           +NSYPLP+S+        I TYM    VPTA+I KS     + APF+ SFSSRGPNP T 
Sbjct: 432 SNSYPLPSSYLETVDGDAIKTYMASNGVPTATIFKSDAVNDSSAPFIVSFSSRGPNPETL 491

Query: 488 NILKPDISGPGVEILAAWPSTVSPSGAEEDNKRVVYNVISGTSMACPHVTAAAAYVKSFH 547
           +ILKPD++ PGVEILAAW      S    D++  +YN+ISGTSM+CPHVTAAA YVK+FH
Sbjct: 492 DILKPDLTAPGVEILAAWSPIAPVSSGVIDSRTTLYNIISGTSMSCPHVTAAAVYVKTFH 551

Query: 548 PTWSPAALKSALITTAFPMSPKRNPDGELAYGAGHINPLGAVHPGLIYNASETDYVKFLC 607
           PTWSPAA+KSAL+TTA P+ P+ N + E AYGAG INPL A+ PGL+Y+A+E DYVKFLC
Sbjct: 552 PTWSPAAIKSALMTTATPLKPEINVEAEFAYGAGQINPLKAISPGLVYDANEFDYVKFLC 611

Query: 608 GQGYSTESLRRLSDDSASCSANNSGTVFELNYPSFALSTNASNPISQVFRRRVTNVGSSY 667
           GQGY+++ ++ LS+D+  C++ N G V++LNYPSFALS+  S  I+Q F R +T+V S+ 
Sbjct: 612 GQGYTSDMVQSLSNDNTICNSANIGRVWDLNYPSFALSSTPSQSINQFFTRTLTSVDSN- 671

Query: 668 ATSYKAVIVNPWRDLKITVKPSVLLFKRLGEELRFEVRIGGKI-RKGIESAALVWDDGKH 727
           A++Y + I+   + L ITV P VL F  +GE+  F + I G I    I SA+LVW D  H
Sbjct: 672 ASTYTSTILGAPQGLTITVNPKVLSFSGIGEKKTFTLTIQGTIDPTTIVSASLVWSDSSH 731

Query: 728 KVRSPITVF 729
            VRSPIT++
Sbjct: 732 DVRSPITIY 739

BLAST of Cp4.1LG01g14250 vs. TAIR10
Match: AT5G59190.1 (AT5G59190.1 subtilase family protein)

HSP 1 Score: 637.5 bits (1643), Expect = 1.0e-182
Identity = 342/703 (48.65%), Postives = 456/703 (64.86%), Query Frame = 1

Query: 36  MGSHPKGKVSTSSHHIRFLTETIGSNFAPRSLIRSYKRSFNGFVAKLTEAEAKKVSEMEG 95
           MG+ P+ K S  SHH+  L + +G+  A   L+RSYKRSFNGF A L++AE++K+  M+ 
Sbjct: 1   MGTLPEIKYSPPSHHLSILQKLVGTIAASHLLVRSYKRSFNGFAANLSQAESQKLQNMKE 60

Query: 96  VISVFPNGKKQLHTTRSWDFMGFSEQVKRIPAVESNIIVGVFDSGIWPESPSFDDTGYGP 155
           V+SVFP+   +L TTRSWDF+GF E+ +R    ES++IVGV DSGIWPES SFDD G+GP
Sbjct: 61  VVSVFPSKSHELTTTRSWDFVGFGEKARRESVKESDVIVGVIDSGIWPESESFDDEGFGP 120

Query: 156 PPAKWRGRCEASANFSCNNKIIGARSYRSNGKHLENDIKGPIDSDGHGTHTASTVAGGLV 215
           PP KW+G C+    F+CNNK+IGAR Y               D +GHGTHTAST AG  V
Sbjct: 121 PPKKWKGSCKGGLKFACNNKLIGARFYNKFADSAR-------DEEGHGTHTASTAAGNAV 180

Query: 216 RQASMLGLGLGTARGGVPSARIAAYKVCWSDGCTDADILAAFDDAIADGVDIISGSLGGS 275
           + AS  GL  GTARGGVPSARIAAYKVC++  C D DILAAFDDAIADGVD+IS S+   
Sbjct: 181 QAASFYGLAQGTARGGVPSARIAAYKVCFNR-CNDVDILAAFDDAIADGVDVISISISAD 240

Query: 276 SVKDYFNDSIAIGSFHAMKKGILTTLAVGNNGPEFTTIVNFSPWSLSVAASTTDRKFETK 335
            V +  N S+AIGSFHAM +GI+T  + GNNGP+  ++ N SPW ++VAAS TDR+F  +
Sbjct: 241 YVSNLLNASVAIGSFHAMMRGIITAGSAGNNGPDQGSVANVSPWMITVAASGTDRQFIDR 300

Query: 336 VKLGDGREFNGVSVNTFDLNGTQIPLVYAGDIPKAPFDRSVSRMCFENTVDKERVKGKIV 395
           V LG+G+   G+SVNTF+LNGT+ P+VY  ++ +    ++ +  C    VD E VKGKIV
Sbjct: 301 VVLGNGKALTGISVNTFNLNGTKFPIVYGQNVSRN-CSQAQAGYCSSGCVDSELVKGKIV 360

Query: 396 VCDSLAVPGGVVAVEGAAGIIMQDESPQDDTNSYPLPASHNTL-----IITYMMIYRVPT 455
           +CD   +      + GA G+I+Q+    D     P PAS         I +Y+     P 
Sbjct: 361 LCDDF-LGYREAYLAGAIGVIVQNTLLPDSAFVVPFPASSLGFEDYKSIKSYIESAEPPQ 420

Query: 456 ASIMKSRGRKRNRAPFVASFSSRGPNPITPNILKPDISGPGVEILAAWPSTVSPSGA--E 515
           A I+++       AP+V SFSSRGP+ +  N+LKPD+S PG+EILAA+    SPS     
Sbjct: 421 AEILRTEEIVDREAPYVPSFSSRGPSFVIQNLLKPDVSAPGLEILAAFSPVASPSSFLNP 480

Query: 516 EDNKRVVYNVISGTSMACPHVTAAAAYVKSFHPTWSPAALKSALITTAFPMSPKRNPDGE 575
           ED + V Y+V+SGTSMACPHV   AAYVKSFHP WSP+A+KSA++TTA PM+ K+NP+ E
Sbjct: 481 EDKRSVRYSVMSGTSMACPHVAGVAAYVKSFHPDWSPSAIKSAIMTTATPMNLKKNPEQE 540

Query: 576 LAYGAGHINPLGAVHPGLIYNASETDYVKFLCGQGYSTESLRRLSDDSASCSANNSGTVF 635
            AYG+G INP  A  PGL+Y     DY+K LC +G+ + +L   S  + +CS      V 
Sbjct: 541 FAYGSGQINPTKASDPGLVYEVETEDYLKMLCAEGFDSTTLTTTSGQNVTCSERTE--VK 600

Query: 636 ELNYPSFALSTNASNPISQVFRRRVTNVGSSYATSYKAVIVNPWRDLKITVKPSVLLFKR 695
           +LNYP+     ++ +P +  F+R VTNVG   +T YKA +V    +L+I+++P +L F  
Sbjct: 601 DLNYPTMTTFVSSLDPFNVTFKRTVTNVGFPNST-YKASVVPLQPELQISIEPEILRFGF 660

Query: 696 LGEELRFEVRIGGKIRK--GIESAALVWDDGKHKVRSPITVFN 730
           L E+  F V I GK  K     S+++VW DG H VRSPI  ++
Sbjct: 661 LEEKKSFVVTISGKELKDGSFVSSSVVWSDGSHSVRSPIVAYS 690

BLAST of Cp4.1LG01g14250 vs. TAIR10
Match: AT3G46850.1 (AT3G46850.1 Subtilase family protein)

HSP 1 Score: 625.9 bits (1613), Expect = 3.1e-179
Identity = 352/741 (47.50%), Postives = 468/741 (63.16%), Query Frame = 1

Query: 3   SLSTTLLFLSFCFSLLFFSSFSQEDPQK-TYIVYMGSHP-KGKVSTSSHHIRFLTETIGS 62
           ++S  LL   F   ++ F+S  ++D  K  YIVYMG+ P +      SHH   L +  G 
Sbjct: 4   AVSYCLLSCIFALLVVSFASAGKDDQDKQVYIVYMGALPSRVDYMPMSHHTSILQDVTGE 63

Query: 63  NFAPRSLIRSYKRSFNGFVAKLTEAEAKKVSEMEGVISVFPNGKKQLHTTRSWDFMGFSE 122
           +     L+R+YKRSFNGF A+LTE+E + ++ M+ V+SVFP+    L TT SW+FMG  E
Sbjct: 64  SSIQDRLVRNYKRSFNGFAARLTESEREILASMDEVVSVFPSKNLNLQTTTSWNFMGLKE 123

Query: 123 --QVKRIPAVESNIIVGVFDSGIWPESPSFDDTGYGPPPAKWRGRCEASANFSCNNKIIG 182
             + KR P +ES+ I+GV DSGI+PES SF   G+GPPP KW+G C+   NF+CNNK+IG
Sbjct: 124 GKRTKRNPLIESDTIIGVIDSGIYPESDSFSGKGFGPPPKKWKGVCKGGTNFTCNNKLIG 183

Query: 183 ARSYRSNGKHLENDIKGPIDSDGHGTHTASTVAGGLVRQASMLGLGLGTARGGVPSARIA 242
           AR Y      LE   +   D+ GHG+HTAS  AG  V+  S  GLG GT RGGVP+ARIA
Sbjct: 184 ARYYTPK---LEGFPESARDNTGHGSHTASIAAGNAVKHVSFYGLGNGTVRGGVPAARIA 243

Query: 243 AYKVCWSD--GCTDADILAAFDDAIADGVDIISGSLGGSSVKDYFNDSIAIGSFHAMKKG 302
            YKVC      CT   ILAAFDDAIAD VDII+ SLG  +V  +  D++AIG+FHAM KG
Sbjct: 244 VYKVCDPGVIRCTSDGILAAFDDAIADKVDIITVSLGADAVGTFEEDTLAIGAFHAMAKG 303

Query: 303 ILTTLAVGNNGPEFTTIVNFSPWSLSVAASTTDRKFETKVKLGDGREFNGVSVNTFDLNG 362
           ILT    GNNGPE  TIV+ +PW  +VAAS  +R F TKV LG+G+   G SVN+FDLNG
Sbjct: 304 ILTVNGAGNNGPERRTIVSMAPWLFTVAASNMNRAFITKVVLGNGKTIVGRSVNSFDLNG 363

Query: 363 TQIPLVYAGDIPKAPFDRSVSRMCFENTVDKERVKGKIVVCDSLAVPGGVVAVEGAAGII 422
            + PLVY G    +  D S +  C    +D +RVKGKIV+CD+   PG   A+   A I+
Sbjct: 364 KKYPLVY-GKSASSRCDASSAGFCSPGCLDSKRVKGKIVLCDTQRNPGEAQAMGAVASIV 423

Query: 423 MQDESPQDDTNSYP---LPASHNTLIITYMMIYRVPTASIMKSRGRKRNRAPFVASFSSR 482
                      S+P   L      ++++Y+   + P A+++KS      +AP VAS+SSR
Sbjct: 424 RNPYEDAASVFSFPVSVLSEDDYNIVLSYVNSTKNPKAAVLKSETIFNQKAPVVASYSSR 483

Query: 483 GPNPITPNILKPDISGPGVEILAAWPSTVSPSGAEEDNKRVVYNVISGTSMACPHVTAAA 542
           GPNP+  +ILKPDI+ PG EILAA+   V PS  E D + V Y VISGTSM+CPHV   A
Sbjct: 484 GPNPLIHDILKPDITAPGSEILAAYSPYVPPS--ESDTRHVKYTVISGTSMSCPHVAGVA 543

Query: 543 AYVKSFHPTWSPAALKSALITTAFPMSPKRNPDGEL---AYGAGHINPLGAVHPGLIYNA 602
           AY+K+FHP WSP+ ++SA++TTA+PM+   +P  EL   AYGAGH++P+ A+HPGL+Y A
Sbjct: 544 AYIKTFHPLWSPSMIQSAIMTTAWPMNASTSPSNELAEFAYGAGHVDPIAAIHPGLVYEA 603

Query: 603 SETDYVKFLCGQGYSTESLRRLSDDSASCSANNSGTVF-ELNYPSFALSTNASNPISQVF 662
           +++D++ FLCG  Y+ + LR +S DS+SC+   + ++   LNYPS +   + + P    F
Sbjct: 604 NKSDHITFLCGFNYTGKKLRLISGDSSSCTKEQTKSLTRNLNYPSMSAQVSGTKPFKVTF 663

Query: 663 RRRVTNVGSSYATSYKAVIVNPWRDLKITVKPSVLLFKRLGEELRFEVRIGGKIRK--GI 722
           RR VTNVG   AT YKA +V     LK+ V P+VL  K L E+  F V + G   K   +
Sbjct: 664 RRTVTNVGRPNAT-YKAKVVG--SKLKVKVVPAVLSLKSLYEKKSFTVTVSGAGPKAENL 723

Query: 723 ESAALVWDDGKHKVRSPITVF 729
            SA L+W DG H VRSPI V+
Sbjct: 724 VSAQLIWSDGVHFVRSPIVVY 735

BLAST of Cp4.1LG01g14250 vs. TAIR10
Match: AT5G59100.1 (AT5G59100.1 Subtilisin-like serine endopeptidase family protein)

HSP 1 Score: 620.9 bits (1600), Expect = 9.9e-178
Identity = 342/741 (46.15%), Postives = 470/741 (63.43%), Query Frame = 1

Query: 6   TTLLFLSFCFSLLFFSSFSQEDP----QKTYIVYMGSHPKGKVSTS-SHHIRFLTETIGS 65
           TT +FL     +L  SS S +      Q+ YIVY+GS P  +  T  S H+  L E  G 
Sbjct: 5   TTFIFLFSSLLVLSLSSVSADKDDHGDQQVYIVYLGSLPSREEYTPMSDHMSILQEITGE 64

Query: 66  NFAPRSLIRSYKRSFNGFVAKLTEAEAKKVSEMEGVISVFPNGKKQLHTTRSWDFMGFSE 125
           +     L+RSYK+SFNGF A+LTE+E K+++ ME V+SVFP+ K +L TT SW+FMG  E
Sbjct: 65  SLIENRLVRSYKKSFNGFAARLTESERKRLAGMERVVSVFPSRKLKLQTTSSWNFMGLKE 124

Query: 126 QVK--RIPAVESNIIVGVFDSGIWPESPSFDDTGYGPPPAKWRGRCEASANFSCNNKIIG 185
            +K  R  ++ES+ I+GV DSGI+PES SF D G+GPPP KW+G C    NF+CNNK+IG
Sbjct: 125 GIKTKRTRSIESDTIIGVIDSGIYPESDSFSDQGFGPPPKKWKGTCAGGKNFTCNNKVIG 184

Query: 186 ARSYRSNGKHLENDIKGPIDSDGHGTHTASTVAGGLVRQASMLGLGLGTARGGVPSARIA 245
           AR Y +  K  +       D  GHGTHTAS  AG  V  ++  GLG GTARGGVP+ARIA
Sbjct: 185 ARDYTAKSKANQT----ARDYSGHGTHTASIAAGNAVANSNFYGLGNGTARGGVPAARIA 244

Query: 246 AYKVCWSDGCTDADILAAFDDAIADGVDIISGSLGGSSVKDYFNDSIAIGSFHAMKKGIL 305
            YKVC ++GC    +++AFDDAIADGVD+IS S+   ++  +  D IAIG+FHAM  G+L
Sbjct: 245 VYKVCDNEGCDGEAMMSAFDDAIADGVDVISISIVLDNIPPFEEDPIAIGAFHAMAVGVL 304

Query: 306 TTLAVGNNGPEFTTIVNFSPWSLSVAASTTDRKFETKVKLGDGREFNGVSVNTFDLNGTQ 365
           T  A GNNGP+ +T+ + +PW  SVAAS T+R F  KV LGDG+   G SVNT+D+NGT 
Sbjct: 305 TVNAAGNNGPKISTVTSTAPWVFSVAASVTNRAFMAKVVLGDGKILIGRSVNTYDMNGTN 364

Query: 366 IPLVYAGDIPKAPFDRSVSRMCFENTVDKERVKGKIVVCDSLAVPGGVVAVE--GAAGII 425
            PLVY      +      +R+C    +D + VKGKIV+CDS     G++  +  GA G I
Sbjct: 365 YPLVYGKSAALSTCSVDKARLCEPKCLDGKLVKGKIVLCDSTK---GLIEAQKLGAVGSI 424

Query: 426 MQDESP-QDDTNSYPLPASHN---TLIITYMMIYRVPTASIMKSRGRKRNRAPFVASFSS 485
           +++  P +    S+P+    N     +++YM   + P A+++KS      RAP VASFSS
Sbjct: 425 VKNPEPDRAFIRSFPVSFLSNDDYKSLVSYMNSTKNPKATVLKSEEISNQRAPLVASFSS 484

Query: 486 RGPNPITPNILKPDISGPGVEILAAWPSTVSPSGAEEDNKRVVYNVISGTSMACPHVTAA 545
           RGP+ I  +ILKPDI+ PGVEILAA+    SP+ +E D +RV Y+V+SGTSMACPHV   
Sbjct: 485 RGPSSIVSDILKPDITAPGVEILAAYSPDSSPTESEFDTRRVKYSVLSGTSMACPHVAGV 544

Query: 546 AAYVKSFHPTWSPAALKSALITTAFPMSPKRN--PDGELAYGAGHINPLGAVHPGLIYNA 605
           AAYVK+FHP WSP+ ++SA++TTA+PM+   +     E AYG+GH++P+ A++PGL+Y  
Sbjct: 545 AAYVKTFHPQWSPSMIQSAIMTTAWPMNASGSGFVSTEFAYGSGHVDPIDAINPGLVYEL 604

Query: 606 SETDYVKFLCGQGYSTESLRRLSDDSASCSANNSGTV-FELNYPSFALSTNASNPISQVF 665
           ++ D++ FLCG  Y+++ LR +S D+++C+   S T+   LNYP+ +   + + P +  F
Sbjct: 605 TKADHINFLCGLNYTSDHLRIISGDNSTCTKEISKTLPRNLNYPTMSAKVSGTKPFNITF 664

Query: 666 RRRVTNVGSSYATSYKAVIVNPWRDLKITVKPSVLLFKRLGEELRFEVRIGGKI--RKGI 725
           +R VTNVG   +T    V+  P   L I V P VL  K + E+  F V +       K  
Sbjct: 665 QRTVTNVGMQKSTYNAKVVKFPGSKLSIKVSPRVLSMKSMNEKQSFMVTVSSDSIGTKQP 724

Query: 726 ESAALVWDDGKHKVRSPITVF 729
            SA L+W DG H VRSPI V+
Sbjct: 725 VSANLIWSDGTHNVRSPIIVY 738

BLAST of Cp4.1LG01g14250 vs. TAIR10
Match: AT5G59090.1 (AT5G59090.1 subtilase 4.12)

HSP 1 Score: 619.0 bits (1595), Expect = 3.8e-177
Identity = 344/744 (46.24%), Postives = 462/744 (62.10%), Query Frame = 1

Query: 1   MASLSTTLLFLSFCFSLLF--FSSFSQEDPQKTYIVYMGS-HPKGKVSTSSHHIRFLTET 60
           MA+L+ +    S+   LL    S+   ED Q  YIVYMGS   +     +S H+  L + 
Sbjct: 1   MANLAASTCLYSWLLVLLLSSVSAIIDEDTQ-VYIVYMGSLSSRADYIPTSDHMSILQQV 60

Query: 61  IGSNFAPRSLIRSYKRSFNGFVAKLTEAEAKKVSEMEGVISVFPNGKKQLHTTRSWDFMG 120
            G +     L+RSYKRSFNGF A+LTE+E   ++E+EGV+SVFPN   QLHTT SWDFMG
Sbjct: 61  TGESSIEGRLVRSYKRSFNGFAARLTESERTLIAEIEGVVSVFPNKILQLHTTTSWDFMG 120

Query: 121 FSE--QVKRIPAVESNIIVGVFDSGIWPESPSFDDTGYGPPPAKWRGRCEASANFSCNNK 180
             E    KR  A+ES+ I+GV D+GIWPES SF D G+GPPP KW+G C    NF+CNNK
Sbjct: 121 VKEGKNTKRNLAIESDTIIGVIDTGIWPESKSFSDKGFGPPPKKWKGVCSGGKNFTCNNK 180

Query: 181 IIGARSYRSNGKHLENDIKGPIDSDGHGTHTASTVAGGLVRQASMLGLGLGTARGGVPSA 240
           +IGAR Y S G           D+ GHGTHTAST AG  V+  S  G+G GT RGGVP++
Sbjct: 181 LIGARDYTSEGTR---------DTSGHGTHTASTAAGNAVKDTSFFGIGNGTVRGGVPAS 240

Query: 241 RIAAYKVCWSDGCTDADILAAFDDAIADGVDIISGSLGGSSVKDYFNDSIAIGSFHAMKK 300
           RIAAYKVC   GC+   +L++FDDAIADGVD+I+ S+G      + +D IAIG+FHAM K
Sbjct: 241 RIAAYKVCTDSGCSSEALLSSFDDAIADGVDLITISIGFQFPSIFEDDPIAIGAFHAMAK 300

Query: 301 GILTTLAVGNNGPEFTTIVNFSPWSLSVAASTTDRKFETKVKLGDGREFNGVSVNTFDLN 360
           GILT  + GN+GP+ TT+ + +PW  +VAASTT+R F TKV LG+G+   G SVN FD+ 
Sbjct: 301 GILTVSSAGNSGPKPTTVSHVAPWIFTVAASTTNRGFITKVVLGNGKTLAGRSVNAFDMK 360

Query: 361 GTQIPLVYAGDIPKAPFDRSVSRMCFENTVDKERVKGKIVVCDSLAVPGGVVAVEGAAGI 420
           G + PLVY      +  D   + +C    ++K RVKGKI+VC     P G    +    I
Sbjct: 361 GKKYPLVYGKSAASSACDAKTAALCAPACLNKSRVKGKILVCGG---PSGYKIAKSVGAI 420

Query: 421 IMQDESPQDD---TNSYP---LPASHNTLIITYMMIYRVPTASIMKSRGRKRNRAPFVAS 480
            + D+SP+ D   T+  P   L A     +++Y+     P A+++K+       +P +AS
Sbjct: 421 AIIDKSPRPDVAFTHHLPASGLKAKDFKSLVSYIESQDSPQAAVLKTETIFNRTSPVIAS 480

Query: 481 FSSRGPNPITPNILKPDISGPGVEILAAWPSTVSPSGAEEDNKRVVYNVISGTSMACPHV 540
           FSSRGPN I  +ILKPDI+ PGVEILAA+     PS  E+D +RV Y+V SGTSMACPHV
Sbjct: 481 FSSRGPNTIAVDILKPDITAPGVEILAAFSPNGEPS--EDDTRRVKYSVFSGTSMACPHV 540

Query: 541 TAAAAYVKSFHPTWSPAALKSALITTAFPMSPKRN--PDGELAYGAGHINPLGAVHPGLI 600
              AAYVK+F+P WSP+ ++SA++TTA+P+  K       E AYGAGH++P+ A++PGL+
Sbjct: 541 AGVAAYVKTFYPRWSPSMIQSAIMTTAWPVKAKGRGIASTEFAYGAGHVDPMAALNPGLV 600

Query: 601 YNASETDYVKFLCGQGYSTESLRRLSDDSASCSANNSGTVFELNYPSF-ALSTNASNPIS 660
           Y   + D++ FLCG  Y++++L+ +S D+  CS  N      LNYPS  A  +   +  S
Sbjct: 601 YELDKADHIAFLCGMNYTSKTLKIISGDTVKCSKKNKILPRNLNYPSMSAKLSGTDSTFS 660

Query: 661 QVFRRRVTNVGSSYATSYKAVIVNPWRDLKITVKPSVLLFKRLGEELRFEVRI-GGKIRK 720
             F R +TNVG+  +T    V+      L I V PSVL FK + E+  F V + G  +  
Sbjct: 661 VTFNRTLTNVGTPNSTYKSKVVAGHGSKLSIKVTPSVLYFKTVNEKQSFSVTVTGSDVDS 720

Query: 721 GIESAA-LVWDDGKHKVRSPITVF 729
            + S+A L+W DG H VRSPI V+
Sbjct: 721 EVPSSANLIWSDGTHNVRSPIVVY 729

BLAST of Cp4.1LG01g14250 vs. TAIR10
Match: AT5G59120.1 (AT5G59120.1 subtilase 4.13)

HSP 1 Score: 619.0 bits (1595), Expect = 3.8e-177
Identity = 344/747 (46.05%), Postives = 471/747 (63.05%), Query Frame = 1

Query: 1   MASLSTTLLFLSFCFSLLFFSSFSQ-EDPQKTYIVYMGS-HPKGKVSTSSHHIRFLTETI 60
           MA+L+ +   LS C  +LF SS S   D ++ YIVYMGS   +   + +S H+  L E  
Sbjct: 1   MATLAASSSLLS-CLLVLFLSSVSAVTDDKQVYIVYMGSLSSRADYTPTSDHMNILQEVT 60

Query: 61  GSNFAPRSLIRSYKRSFNGFVAKLTEAEAKKVSEMEGVISVFPNGKKQLHTTRSWDFMGF 120
           G +     L+RSYKRSFNGF A+LTE+E ++V++M GV+SVFPN K QL TT SWDFMG 
Sbjct: 61  GESSIEGRLVRSYKRSFNGFAARLTESERERVAKMVGVVSVFPNKKLQLQTTTSWDFMGL 120

Query: 121 SEQVK--RIPAVESNIIVGVFDSGIWPESPSFDDTGYGPPPAKWRGRCEASANFSCNNKI 180
            E +K  R P VES+ I+GV DSGI PES SF D G+GPPP KW+G C    NF+CNNK+
Sbjct: 121 KEGIKTKRNPTVESDTIIGVIDSGITPESQSFSDKGFGPPPQKWKGVCSGGKNFTCNNKL 180

Query: 181 IGARSYRSNGKHLENDIKGPIDSDGHGTHTASTVAGGLVRQASMLGLGLGTARGGVPSAR 240
           IGAR Y S G           D DGHGTHTAST AG  V  AS  G+G GT RGGVP++R
Sbjct: 181 IGARDYTSEGTR---------DMDGHGTHTASTAAGNAVVDASFFGIGNGTVRGGVPASR 240

Query: 241 IAAYKVCWSDGCTDADILAAFDDAIADGVDIISGSLGGSSVKDYFNDSIAIGSFHAMKKG 300
           +AAYKVC   GC+   +L+AFDDAIADGVD+I+ S+G  +   + ND IAIG+FHAM KG
Sbjct: 241 VAAYKVCTPTGCSSEALLSAFDDAIADGVDLITISIGDKTASMFQNDPIAIGAFHAMAKG 300

Query: 301 ILTTLAVGNNGPEFTTIVNFSPWSLSVAASTTDRKFETKVKLGDGREFNGVSVNTFDLNG 360
           +LT  + GN+GP+  ++   +PW L+VAASTT+R F TKV LG+G+   G SVN +++ G
Sbjct: 301 VLTVNSAGNSGPKPISVSGVAPWILTVAASTTNRGFVTKVVLGNGKTLVGKSVNAYEMKG 360

Query: 361 TQIPLVYAGDIPKAPFDRSVSRMCFENTVDKERVKGKIVVCDSLAVPGGVVAVE--GAAG 420
              PLVY      +  D   + +C  + VDK RVKGKI+VC     PGG+  VE  GA G
Sbjct: 361 KDYPLVYGKSAASSACDAESAGLCELSCVDKSRVKGKILVCGG---PGGLKIVESVGAVG 420

Query: 421 IIMQDESPQDDTNSYPLPAS-----HNTLIITYMMIYRVPTASIMKSRGRKRNRAPFVAS 480
           +I +   P D    +PLPA+         +++Y+     P A ++K+       +P +AS
Sbjct: 421 LIYRTPKP-DVAFIHPLPAAGLLTEDFESLVSYLESTDSPQAIVLKTEAIFNRTSPVIAS 480

Query: 481 FSSRGPNPITPNILKPDISGPGVEILAAWPSTVSPSGAEEDNKRVVYNVISGTSMACPHV 540
           FSSRGPN I  +ILKPDI+ PGVEILAA+     PS  ++D + V Y+V+SGTSM+CPHV
Sbjct: 481 FSSRGPNTIAVDILKPDITAPGVEILAAYSPAGEPS--QDDTRHVKYSVLSGTSMSCPHV 540

Query: 541 TAAAAYVKSFHPTWSPAALKSALITTAFPMSPKRN--PDGELAYGAGHINPLGAVHPGLI 600
              AAYVK+F+P WSP+ ++SA++TTA+P++         E AYG+GH++P+ A +PGL+
Sbjct: 541 AGVAAYVKTFNPKWSPSMIQSAIMTTAWPVNATGTGIASTEFAYGSGHVDPIAASNPGLV 600

Query: 601 YNASETDYVKFLCGQGYSTESLRRLSDDSASCSANNSGTVFELNYPSFALSTNASNPISQ 660
           Y   ++D++ FLCG  Y+++ L+ +S ++ +CS         LNYPS +   + S     
Sbjct: 601 YELDKSDHIAFLCGMNYTSQVLKVISGETVTCSEAKKILPRNLNYPSMSAKLSGSGTTFT 660

Query: 661 V-FRRRVTNVGSSYATSYKAVIVNPWRDLKITVKPSVLLFKRLGEELRFEVRI-GGKIRK 720
           V F R +TNVG+  +T    V+      L + + PSVL FK + E+  F V + G  +  
Sbjct: 661 VTFNRTLTNVGTPNSTYTSKVVAGHGSKLDVKITPSVLSFKTVNEKQSFTVTVTGSNLDS 720

Query: 721 GIESAA-LVWDDGKHKVRSPITVFNAN 732
            + S+A L+W DG H VRSPI V+ ++
Sbjct: 721 EVPSSANLIWSDGTHNVRSPIVVYTSD 731

BLAST of Cp4.1LG01g14250 vs. NCBI nr
Match: gi|700197004|gb|KGN52181.1| (hypothetical protein Csa_5G613590 [Cucumis sativus])

HSP 1 Score: 1193.3 bits (3086), Expect = 0.0e+00
Identity = 600/740 (81.08%), Postives = 655/740 (88.51%), Query Frame = 1

Query: 1   MASLSTTLLFLSFCFSLLFFSSFSQE--DPQKTYIVYMGSHPKGKVSTSSHHIRFLTETI 60
           M+SL   LLFLSFC  LLFF S SQ+  D QKTYIVYMGSH KGKVSTSSHHIR L ETI
Sbjct: 1   MSSLIPRLLFLSFCLFLLFFCSNSQDNYDSQKTYIVYMGSHSKGKVSTSSHHIRLLKETI 60

Query: 61  GSNFAPRSLIRSYKRSFNGFVAKLTEAEAKKVSEMEGVISVFPNGKKQLHTTRSWDFMGF 120
           GS+F P SL+ S+KRSFNGFVAKLTEAE KKVSEMEGVISVFPNGKKQLHTTRSWDFMGF
Sbjct: 61  GSSFPPHSLLHSFKRSFNGFVAKLTEAEVKKVSEMEGVISVFPNGKKQLHTTRSWDFMGF 120

Query: 121 SEQVKRIPAVESNIIVGVFDSGIWPESPSFDDTGYGPPPAKWRGRCEASANFSCNNKIIG 180
           SEQVKR+PAVESN+IVGV DSGIWPESPSFD  GYG PPAKW+G CE SANFSCNNKIIG
Sbjct: 121 SEQVKRVPAVESNVIVGVLDSGIWPESPSFDHAGYGSPPAKWKGSCEVSANFSCNNKIIG 180

Query: 181 ARSYRSNGKHLENDIKGPIDSDGHGTHTASTVAGGLVRQASMLGLGLGTARGGVPSARIA 240
           ARSYRSNG++ E DIKGP DSDGHGTHTAS VAGGLVR+ASMLGLGLGTARGGVPSARIA
Sbjct: 181 ARSYRSNGEYPEGDIKGPRDSDGHGTHTASIVAGGLVRRASMLGLGLGTARGGVPSARIA 240

Query: 241 AYKVCWSDGCTDADILAAFDDAIADGVDIISGSLGGSSVKDYFNDSIAIGSFHAMKKGIL 300
           AYKVCWSDGC+DADILAAFDDAIADGVDIISGSLGGS  +DYFNDSIAIGSFHAMKKGIL
Sbjct: 241 AYKVCWSDGCSDADILAAFDDAIADGVDIISGSLGGSGARDYFNDSIAIGSFHAMKKGIL 300

Query: 301 TTLAVGNNGPEFTTIVNFSPWSLSVAASTTDRKFETKVKLGDGREFNGVSVNTFDLNGTQ 360
           T+LAVGNNGP+FTTIVNFSPWSLSVAASTTDRKFETKV+LGDGREF+GVSVNTFD+ G Q
Sbjct: 301 TSLAVGNNGPDFTTIVNFSPWSLSVAASTTDRKFETKVELGDGREFSGVSVNTFDIKGKQ 360

Query: 361 IPLVYAGDIPKAPFDRSVSRMCFENTVDKERVKGKIVVCDSLAVPGGVVAVEGAAGIIMQ 420
           IPLVYAGDIPKAPFD SVSR+CFENTVD + VKGKIVVCDSL VPGGVVAV+GA GIIMQ
Sbjct: 361 IPLVYAGDIPKAPFDSSVSRLCFENTVDLKLVKGKIVVCDSLTVPGGVVAVKGAVGIIMQ 420

Query: 421 DESPQDDTNSYPLPASH-----NTLIITYM-MIYRVPTASIMKSRGRKRNRAPFVASFSS 480
           D+S  DDTNS+P+PASH       L+++Y+     +PTA+I KS  RKR RAP VASFSS
Sbjct: 421 DDSSHDDTNSFPIPASHLGPKAGALVLSYINSTNSIPTATIKKSTERKRKRAPSVASFSS 480

Query: 481 RGPNPITPNILKPDISGPGVEILAAWPSTVSPSGAEEDNKRVVYNVISGTSMACPHVTAA 540
           RGPNPITPNILKPD+SGPGVEILAAW     PSGAEEDNKRV+YN+ISGTSMACPHVTAA
Sbjct: 481 RGPNPITPNILKPDLSGPGVEILAAWSPVSPPSGAEEDNKRVLYNIISGTSMACPHVTAA 540

Query: 541 AAYVKSFHPTWSPAALKSALITTAFPMSPKRNPDGELAYGAGHINPLGAVHPGLIYNASE 600
           AAYVKSFHPTWSP+ALKSALITTAFPMSPK NPD E  YGAGHINPLGAVHPGLIY+ASE
Sbjct: 541 AAYVKSFHPTWSPSALKSALITTAFPMSPKHNPDKEFGYGAGHINPLGAVHPGLIYDASE 600

Query: 601 TDYVKFLCGQGYSTESLRRLSDDSASCSANNSGTVFELNYPSFALSTNASNPISQVFRRR 660
            DYV+FLCGQGY+TE L+ +S+D+ +CS+NNS TVF+LNYPSFALSTN S PI+QV++R 
Sbjct: 601 IDYVQFLCGQGYTTELLQLVSEDNNTCSSNNSDTVFDLNYPSFALSTNISKPINQVYKRT 660

Query: 661 VTNVGSSYATSYKAVIVNPWRDLKITVKPSVLLFKRLGEELRFEVRIGGKIRKGIESAAL 720
           VTNVGS YAT YKA ++NPW++L+I V PSVL FK LGE+  FEV I GKIRK IESA+L
Sbjct: 661 VTNVGSKYAT-YKATVINPWKNLEIKVNPSVLSFKNLGEKQSFEVTIRGKIRKDIESASL 720

Query: 721 VWDDGKHKVRSPITVFNANL 733
           VWDDGKHKVRSPITVF AN+
Sbjct: 721 VWDDGKHKVRSPITVFIANI 739

BLAST of Cp4.1LG01g14250 vs. NCBI nr
Match: gi|778708693|ref|XP_004135365.2| (PREDICTED: LOW QUALITY PROTEIN: cucumisin-like [Cucumis sativus])

HSP 1 Score: 1174.1 bits (3036), Expect = 0.0e+00
Identity = 590/728 (81.04%), Postives = 644/728 (88.46%), Query Frame = 1

Query: 1   MASLSTTLLFLSFCFSLLFFSSFSQE--DPQKTYIVYMGSHPKGKVSTSSHHIRFLTETI 60
           M+SL   LLFLSFC  LLFF S SQ+  D QKTYIVYMGSH KGKVSTSSHHIR L ETI
Sbjct: 1   MSSLIPRLLFLSFCLFLLFFCSNSQDNYDSQKTYIVYMGSHSKGKVSTSSHHIRLLKETI 60

Query: 61  GSNFAPRSLIRSYKRSFNGFVAKLTEAEAKKVSEMEGVISVFPNGKKQLHTTRSWDFMGF 120
           GS+F P SL+ S+KRSFNGFVAKLTEAE KKVSEMEGVISVFPNGKKQLHTTRSWDFMGF
Sbjct: 61  GSSFPPHSLLHSFKRSFNGFVAKLTEAEVKKVSEMEGVISVFPNGKKQLHTTRSWDFMGF 120

Query: 121 SEQVKRIPAVESNIIVGVFDSGIWPESPSFDDTGYGPPPAKWRGRCEASANFSCNNKIIG 180
           SEQVKR+PAVESN+IVGV DSGIWPESPSFD  GYG PPAKW+G CE SANFSCNNKIIG
Sbjct: 121 SEQVKRVPAVESNVIVGVLDSGIWPESPSFDHAGYGSPPAKWKGSCEVSANFSCNNKIIG 180

Query: 181 ARSYRSNGKHLENDIKGPIDSDGHGTHTASTVAGGLVRQASMLGLGLGTARGGVPSARIA 240
           ARSYRSNG++ E DIKGP DSDGHGTHTAS VAGGLVR+ASMLGLGLGTARGGVPSARIA
Sbjct: 181 ARSYRSNGEYPEGDIKGPRDSDGHGTHTASIVAGGLVRRASMLGLGLGTARGGVPSARIA 240

Query: 241 AYKVCWSDGCTDADILAAFDDAIADGVDIISGSLGGSSVKDYFNDSIAIGSFHAMKKGIL 300
           AYKVCWSDGC+DADILAAFDDAIADGVDIISGSLGGS  +DYFNDSIAIGSFHAMKKGIL
Sbjct: 241 AYKVCWSDGCSDADILAAFDDAIADGVDIISGSLGGSGARDYFNDSIAIGSFHAMKKGIL 300

Query: 301 TTLAVGNNGPEFTTIVNFSPWSLSVAASTTDRKFETKVKLGDGREFNGVSVNTFDLNGTQ 360
           T+LAVGNNGP+FTTIVNFSPWSLSVAASTTDRKFETKV+LGDGREF+GVSVNTFD+ G Q
Sbjct: 301 TSLAVGNNGPDFTTIVNFSPWSLSVAASTTDRKFETKVELGDGREFSGVSVNTFDIKGKQ 360

Query: 361 IPLVYAGDIPKAPFDRSVSRMCFENTVDKERVKGKIVVCDSLAVPGGVVAVEGAAGIIMQ 420
           IPLVYAGDIPKAPFD SVSR+CFENTVD + VKGKIVVCDSL VPGGVVAV+GA GIIMQ
Sbjct: 361 IPLVYAGDIPKAPFDSSVSRLCFENTVDLKLVKGKIVVCDSLTVPGGVVAVKGAVGIIMQ 420

Query: 421 DESPQDDTNSYPLPASH-----NTLIITYM-MIYRVPTASIMKSRGRKRNRAPFVASFSS 480
           D+S  DDTNS+P+PASH       L+++Y+     +PTA+I KS  RKR RAP VASFSS
Sbjct: 421 DDSSHDDTNSFPIPASHLGPKAGALVLSYINSTNSIPTATIKKSTERKRKRAPSVASFSS 480

Query: 481 RGPNPITPNILKPDISGPGVEILAAWPSTVSPSGAEEDNKRVVYNVISGTSMACPHVTAA 540
           RGPNPITPNILKPD+SGPGVEILAAW     PSGAEEDNKRV+YN+ISGTSMACPHVTAA
Sbjct: 481 RGPNPITPNILKPDLSGPGVEILAAWSPVSPPSGAEEDNKRVLYNIISGTSMACPHVTAA 540

Query: 541 AAYVKSFHPTWSPAALKSALITTAFPMSPKRNPDGELAYGAGHINPLGAVHPGLIYNASE 600
           AAYVKSFHPTWSP+ALKSALITTAFPMSPK NPD E  YGAGHINPLGAVHPGLIY+ASE
Sbjct: 541 AAYVKSFHPTWSPSALKSALITTAFPMSPKHNPDKEFGYGAGHINPLGAVHPGLIYDASE 600

Query: 601 TDYVKFLCGQGYSTESLRRLSDDSASCSANNSGTVFELNYPSFALSTNASNPISQVFRRR 660
            DYV+FLCGQGY+TE L+ +S+D+ +CS+NNS TVF+LNYPSFALSTN S PI+QV++R 
Sbjct: 601 IDYVQFLCGQGYTTELLQLVSEDNNTCSSNNSDTVFDLNYPSFALSTNISKPINQVYKRT 660

Query: 661 VTNVGSSYATSYKAVIVNPWRDLKITVKPSVLLFKRLGEELRFEVRIGGKIRKGIESAAL 720
           VTNVGS YAT YKA ++NPW++L+I V PSVL FK LGE+  FEV I GKIRK IESA+L
Sbjct: 661 VTNVGSKYAT-YKATVINPWKNLEIKVNPSVLSFKNLGEKQSFEVTIRGKIRKDIESASL 720

BLAST of Cp4.1LG01g14250 vs. NCBI nr
Match: gi|659091821|ref|XP_008446751.1| (PREDICTED: cucumisin-like [Cucumis melo])

HSP 1 Score: 1170.6 bits (3027), Expect = 0.0e+00
Identity = 591/727 (81.29%), Postives = 643/727 (88.45%), Query Frame = 1

Query: 1   MASLSTTLLFLSFCFSLLFFSSFSQED-PQKTYIVYMGSHPKGKVSTSSHHIRFLTETIG 60
           M+SL   LLFL+FC  LLFFSS SQ +  QKTYIVYMGSHPKGKVSTSSHHIR L ETIG
Sbjct: 1   MSSLIPRLLFLNFCLFLLFFSSSSQNNGSQKTYIVYMGSHPKGKVSTSSHHIRLLKETIG 60

Query: 61  SNFAPRSLIRSYKRSFNGFVAKLTEAEAKKVSEMEGVISVFPNGKKQLHTTRSWDFMGFS 120
           S+F P SL+ S+KRSFNGFVAKLTEAEAKKVSEMEGVISVFPNGKKQLHTTRSWDFMGFS
Sbjct: 61  SSFPPHSLLHSFKRSFNGFVAKLTEAEAKKVSEMEGVISVFPNGKKQLHTTRSWDFMGFS 120

Query: 121 EQVKRIPAVESNIIVGVFDSGIWPESPSFDDTGYGPPPAKWRGRCEASANFSCNNKIIGA 180
           EQVKR+PAVESN+IVGV DSGIWPESPSFD TGYGPPPAKW+G CE SANFSCNNKIIGA
Sbjct: 121 EQVKRVPAVESNVIVGVLDSGIWPESPSFDHTGYGPPPAKWKGSCEVSANFSCNNKIIGA 180

Query: 181 RSYRSNGKHLENDIKGPIDSDGHGTHTASTVAGGLVRQASMLGLGLGTARGGVPSARIAA 240
           RSYRSNG++ E DIKGP DSDGHGTHTAS VAGGLVRQA+MLGLGLGTARGGVPSARIAA
Sbjct: 181 RSYRSNGEYPEGDIKGPRDSDGHGTHTASIVAGGLVRQANMLGLGLGTARGGVPSARIAA 240

Query: 241 YKVCWSDGCTDADILAAFDDAIADGVDIISGSLGGSSVKDYFNDSIAIGSFHAMKKGILT 300
           YKVCWSDGC+DADILAAFDDAIADGVDIISGSLGGS  +DYFNDSIAIGSFHAMKKGILT
Sbjct: 241 YKVCWSDGCSDADILAAFDDAIADGVDIISGSLGGSGFRDYFNDSIAIGSFHAMKKGILT 300

Query: 301 TLAVGNNGPEFTTIVNFSPWSLSVAASTTDRKFETKVKLGDGREFNGVSVNTFDLNGTQI 360
           +LAVGNNGP+FTTIVNFSPWSLSVAASTTDRKFETKV+LGDGREFNGVSVNTFD+ G QI
Sbjct: 301 SLAVGNNGPDFTTIVNFSPWSLSVAASTTDRKFETKVELGDGREFNGVSVNTFDIKGKQI 360

Query: 361 PLVYAGDIPKAPFDRSVSRMCFENTVDKERVKGKIVVCDSLAVPGGVVAVEGAAGIIMQD 420
           PLVYAGDIPKAPFD SVSR+CFENT+D + VKGKIVVCDSL VPGGV AV+GA GIIMQD
Sbjct: 361 PLVYAGDIPKAPFDSSVSRLCFENTIDLKLVKGKIVVCDSLTVPGGVAAVKGAVGIIMQD 420

Query: 421 ESPQDDTNSYPLPASH-----NTLIITYM-MIYRVPTASIMKSRGRKRNRAPFVASFSSR 480
           +S  DDTNS+P+PASH       LI++Y+     +PTA+I KS  RKR RAP VASFSSR
Sbjct: 421 DSSPDDTNSFPIPASHLGPEAGALILSYINSTNSIPTATIKKSTERKRKRAPSVASFSSR 480

Query: 481 GPNPITPNILKPDISGPGVEILAAWPSTVSPSGAEEDNKRVVYNVISGTSMACPHVTAAA 540
           GPNPITPNILKPD+SGPGVEILAAW     PSGAEED+KRV+YN+ISGTSMACPHVTAAA
Sbjct: 481 GPNPITPNILKPDLSGPGVEILAAWSPISPPSGAEEDSKRVLYNIISGTSMACPHVTAAA 540

Query: 541 AYVKSFHPTWSPAALKSALITTAFPMSPKRNPDGELAYGAGHINPLGAVHPGLIYNASET 600
           AYVKSFHPTWSP+ALKSALITTAF MS K N D E  YGAGHINPLGAVHPGLIY+ASE 
Sbjct: 541 AYVKSFHPTWSPSALKSALITTAFSMSSKHNLDKEFGYGAGHINPLGAVHPGLIYDASEI 600

Query: 601 DYVKFLCGQGYSTESLRRLSDDSASCSANNSGTVFELNYPSFALSTNASNPISQVFRRRV 660
           DYV FLCGQGY+TE L+++S+D+ +CS+NNS TVF+LNYPSFALSTN S PI+QV+RR V
Sbjct: 601 DYVHFLCGQGYTTELLQQVSEDNNTCSSNNSDTVFDLNYPSFALSTNISKPINQVYRRTV 660

Query: 661 TNVGSSYATSYKAVIVNPWRDLKITVKPSVLLFKRLGEELRFEVRIGGKIRKGIESAALV 720
           TNVGS  AT YKA I+NPW++L+I V PSVL FK LGEE  FEV I GKIRK IESA+LV
Sbjct: 661 TNVGSKSAT-YKATIINPWKNLEIKVNPSVLSFKNLGEEQSFEVTIKGKIRKNIESASLV 720

BLAST of Cp4.1LG01g14250 vs. NCBI nr
Match: gi|700197003|gb|KGN52180.1| (hypothetical protein Csa_5G613580 [Cucumis sativus])

HSP 1 Score: 1085.1 bits (2805), Expect = 0.0e+00
Identity = 543/740 (73.38%), Postives = 624/740 (84.32%), Query Frame = 1

Query: 1   MASLSTTLLFLSFCFSLLFFSSFSQED-PQKTYIVYMGSHPKGKVSTSSHHIRFLTETIG 60
           M+ L + LLFL+FCF LLFFS+ SQ++  QKTYIVYMG+HPKGK STSSHH+R L E+IG
Sbjct: 1   MSPLISKLLFLNFCFFLLFFSTNSQDNGSQKTYIVYMGNHPKGKPSTSSHHMRLLKESIG 60

Query: 61  SNFAPRSLIRSYKRSFNGFVAKLTEAEAKKVSEMEGVISVFPNGKKQLHTTRSWDFMGFS 120
           S+F P SL+ SYKRSFNGFVAK+TE EAKKVSEMEGVISVFPNGKKQLHTTRSW+FMGFS
Sbjct: 61  SSFPPNSLLHSYKRSFNGFVAKMTEDEAKKVSEMEGVISVFPNGKKQLHTTRSWNFMGFS 120

Query: 121 EQVKRIPAVESNIIVGVFDSGIWPESPSFDDTGYGPPPAKWRGRCEASANFSCNNKIIGA 180
           EQVKR+P VES+IIVGVFD+GIWPESPSFDDTGYGPPPAKW+G CE SANFSCNNKIIGA
Sbjct: 121 EQVKRVPMVESDIIVGVFDTGIWPESPSFDDTGYGPPPAKWKGSCEVSANFSCNNKIIGA 180

Query: 181 RSYRSNGKHLENDIKGPIDSDGHGTHTASTVAGGLVRQASMLGLGLGTARGGVPSARIAA 240
           RSY S+G H E D++GPIDS+GHGTHTASTVAGGLVRQA+MLGLGLGTARGGVPSARIA 
Sbjct: 181 RSYHSSGPHPEGDLEGPIDSNGHGTHTASTVAGGLVRQANMLGLGLGTARGGVPSARIAV 240

Query: 241 YKVCWSDGCTDADILAAFDDAIADGVDIISGSLGGSSVKDYFNDSIAIGSFHAMKKGILT 300
           YK+CWSD C+DADILAAFDDAIADGVDI+S S+ G   K+YFNDS+AIGSFHAMKKGIL+
Sbjct: 241 YKICWSDNCSDADILAAFDDAIADGVDILSVSVAGPGFKNYFNDSMAIGSFHAMKKGILS 300

Query: 301 TLAVGNNGPEFTTIVNFSPWSLSVAASTTDRKFETKVKLGDGREFNGVSVNTFDLNGTQI 360
           + A GN GP   ++ N+SPWSL+VAASTTDR  ET V+LGDGRE  GV++NTFD+ G Q+
Sbjct: 301 SFAAGNTGPGSASVANYSPWSLTVAASTTDRVLETVVELGDGRELKGVTINTFDMKGKQV 360

Query: 361 PLVYAGDIPKAPFDRSVSRMCFENTVDKERVKGKIVVCDSLAV-PGGVVAVEGAAGIIMQ 420
           PLVY GDIPKA    S S  C  N+VD +  KGKIV+CD +   P   VAV+GA GIIMQ
Sbjct: 361 PLVYGGDIPKANTSSSFSSQCLRNSVDLKLAKGKIVMCDMITTSPAEAVAVKGAVGIIMQ 420

Query: 421 DESPQDDTNSYPLPASH-----NTLIITYM-MIYRVPTASIMKSRGRKRNRAPFVASFSS 480
           ++SP+D T S+P+PASH       LI++Y+     +PTA+I KS  RKR RAP VASFSS
Sbjct: 421 NDSPKDRTFSFPIPASHIDTKSGALILSYINSTNSIPTATIKKSIERKRRRAPSVASFSS 480

Query: 481 RGPNPITPNILKPDISGPGVEILAAWPSTVSPSGAEEDNKRVVYNVISGTSMACPHVTAA 540
           RGPNP+TPNILKPD+SGPGVEILAAWP   SPSGA EDNKRV+YN+ISGTSMACPHVTA 
Sbjct: 481 RGPNPVTPNILKPDLSGPGVEILAAWPPIASPSGAVEDNKRVLYNIISGTSMACPHVTAV 540

Query: 541 AAYVKSFHPTWSPAALKSALITTAFPMSPKRNPDGELAYGAGHINPLGAVHPGLIYNASE 600
           AAYVKSFHPTWSPAALKSAL+TTAFPMSPKRN D E AYGAGH+NPLGAVHPGLIY+ASE
Sbjct: 541 AAYVKSFHPTWSPAALKSALMTTAFPMSPKRNQDKEFAYGAGHLNPLGAVHPGLIYDASE 600

Query: 601 TDYVKFLCGQGYSTESLRRLSDDSASCSANNSGTVFELNYPSFALSTNASNPISQVFRRR 660
            DYV+FLCGQGY+TE L+ +SD S +CS+N+S TVF+LNYPSFALSTN S PI+QV+RR 
Sbjct: 601 IDYVRFLCGQGYTTELLQLVSDGSNTCSSNDSDTVFDLNYPSFALSTNISVPINQVYRRT 660

Query: 661 VTNVGSSYATSYKAVIVNPWRDLKITVKPSVLLFKRLGEELRFEVRIGGKIRKGIESAAL 720
           VTN+GS  A  YKA I+NPW++L I V PSVL F  LGE+  FEV I GKIR+ IESA+L
Sbjct: 661 VTNIGSRSA-MYKATIINPWKNLDIKVNPSVLSFTSLGEKQSFEVTIRGKIRRNIESASL 720

Query: 721 VWDDGKHKVRSPITVFNANL 733
           VW+DGKHKVRSPITVF+A +
Sbjct: 721 VWNDGKHKVRSPITVFDATI 739

BLAST of Cp4.1LG01g14250 vs. NCBI nr
Match: gi|659074279|ref|XP_008437519.1| (PREDICTED: cucumisin-like isoform X1 [Cucumis melo])

HSP 1 Score: 994.6 bits (2570), Expect = 9.3e-287
Identity = 514/745 (68.99%), Postives = 602/745 (80.81%), Query Frame = 1

Query: 1   MASLSTTLLFLSFCFSLLFFSSFSQEDPQ--KTYIVYMGSHPKGKVST----SSHHIRFL 60
           M+SLS  LLFL FCFSLLFFSS S+ED Q  KTYIVYMGSHPK +VS+    S HH+R L
Sbjct: 1   MSSLSR-LLFLCFCFSLLFFSSLSEEDDQYRKTYIVYMGSHPKDQVSSTPLSSHHHMRLL 60

Query: 61  TETIGSNFAPRSLIRSYKRSFNGFVAKLTEAEAKKVSEMEGVISVFPNGKKQLHTTRSWD 120
            E IGS FAP  L+ SYKRSFNGFVAKLTE EAKKVSEMEGVISVFPN + QLHTTRSWD
Sbjct: 61  QEAIGSTFAPHCLLHSYKRSFNGFVAKLTETEAKKVSEMEGVISVFPNEELQLHTTRSWD 120

Query: 121 FMGFSEQVKRIPAVESNIIVGVFDSGIWPESPSFDDTGYGPPPAKWRGRCEASANFSCNN 180
           FMGFSEQV+R+P+VES+IIVGVFD+GIWPESPSF D GYGPPP KW+G CE S NFSCNN
Sbjct: 121 FMGFSEQVERVPSVESDIIVGVFDTGIWPESPSFLDHGYGPPPPKWKGSCEVSLNFSCNN 180

Query: 181 KIIGARSYRSNGKHLENDIKGPIDSDGHGTHTASTVAGGLVRQASMLGLGLGTARGGVPS 240
           KIIGA+SYRS+G++  +DIKGP DS+GHGTH ASTVAGGLVRQASMLGLG GTARGGVPS
Sbjct: 181 KIIGAQSYRSDGRYPIDDIKGPRDSNGHGTHAASTVAGGLVRQASMLGLGTGTARGGVPS 240

Query: 241 ARIAAYKVCWSDGCTDADILAAFDDAIADGVDIISGSLGGS-SVKDYFNDSIAIGSFHAM 300
           ARIAAYKVCWSD C+ AD+LAAFDDAIADGVDIIS S+G    + +YFND IAIG+FHAM
Sbjct: 241 ARIAAYKVCWSDTCSGADVLAAFDDAIADGVDIISLSVGPKRPIPNYFNDPIAIGTFHAM 300

Query: 301 KKGILTTLAVGNNGPEFTTIVNFSPWSLSVAASTTDRKFETKVKLGDGREFNGVSVNTFD 360
           + GILT+ + GN GP   T+ NFSPW+LSVAASTTDR+F T V+LGDGR+FNGV++NTFD
Sbjct: 301 RNGILTSSSAGNEGPRPFTLTNFSPWALSVAASTTDRRFLTAVQLGDGRKFNGVTINTFD 360

Query: 361 LNGTQIPLVYAGDIPKAP--FDRSVSRMCFENTVDKERVKGKIVVCDSLAVPGGVVAVEG 420
           LNGTQ PLV+AG+IP     F+ S+SR C  N+VD+E VKGKI VCDS+ +P  V ++E 
Sbjct: 361 LNGTQYPLVHAGNIPNVTGGFNGSISRFCLGNSVDRELVKGKIAVCDSIVLPSDVGSLES 420

Query: 421 AAGIIMQDESPQDDTNSYPLPASH-----NTLIITYMMIYRVPTASIMKSRGRKRNRAPF 480
           A GIIMQD SP+D T ++PLPASH       LI +Y+ + R+PTA+I+KS   K   AP 
Sbjct: 421 ALGIIMQDGSPKDLTAAFPLPASHLGTQQRPLISSYLNLTRIPTATILKSTELKLEAAPL 480

Query: 481 VASFSSRGPNPITPNILKPDISGPGVEILAAWPSTVSPSGAEEDNKRVVYNVISGTSMAC 540
           VASFSSRGPNP TP+ILKPD+ GPGVEILAAW    SPS A+ DN+++++N+ISGTSMAC
Sbjct: 481 VASFSSRGPNPTTPDILKPDVIGPGVEILAAWSPIRSPSDAKGDNRKLLFNIISGTSMAC 540

Query: 541 PHVTAAAAYVKSFHPTWSPAALKSALITTAFPMSPKRNPDGELAYGAGHINPLGAVHPGL 600
           PH TA AAYVKSFHP+WSPAALKSALITTAFPM     P+ E AYG+GHINPLGAV+PGL
Sbjct: 541 PHATAVAAYVKSFHPSWSPAALKSALITTAFPMRGDLYPEAEFAYGSGHINPLGAVNPGL 600

Query: 601 IYNASETDYVKFLCGQGYSTESLRRLSDDSASCSANNSGTVFELNYPSFALSTNASNPIS 660
           IYNASE DY++FLCG+GY+T  LR ++ D+++CS  NSG V++LNY SFAL T  S P S
Sbjct: 601 IYNASEIDYIRFLCGEGYNTTLLRIITKDNSTCSPTNSGLVYDLNYHSFALFTYISTPFS 660

Query: 661 QVFRRRVTNVGSSYATSYKAVIVNPWRDLKITVKPSVLLFKRLGEELRFEVRIGGKIRKG 720
           QV++RRVTNVGS+ +T YKA I  P  +L ITV PS L FK L EEL+FEV I GKI   
Sbjct: 661 QVYKRRVTNVGSTNST-YKATIFAP-SELNITVNPSTLSFKALEEELKFEVTIEGKINGS 720

Query: 721 IESAALVWDDGKHKVRSPITVFNAN 732
           I SA+LVWDDG HKVRSPI VF+++
Sbjct: 721 IASASLVWDDGVHKVRSPIIVFDSD 742

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
CUCM1_CUCME2.7e-22555.51Cucumisin OS=Cucumis melo PE=1 SV=1[more]
SBT43_ARATH2.7e-18548.04Subtilisin-like protease SBT4.3 OS=Arabidopsis thaliana GN=SBT4.3 PE=3 SV=1[more]
SBT46_ARATH5.4e-17847.50Subtilisin-like protease SBT4.6 OS=Arabidopsis thaliana GN=SBT4.6 PE=3 SV=1[more]
SBT44_ARATH1.8e-17646.15Subtilisin-like protease SBT4.4 OS=Arabidopsis thaliana GN=SBT4.4 PE=2 SV=1[more]
SBT4C_ARATH6.7e-17646.24Subtilisin-like protease SBT4.12 OS=Arabidopsis thaliana GN=SBT4.12 PE=2 SV=1[more]
Match NameE-valueIdentityDescription
A0A0A0KWL9_CUCSA0.0e+0081.08Uncharacterized protein OS=Cucumis sativus GN=Csa_5G613590 PE=4 SV=1[more]
A0A0A0KRC4_CUCSA0.0e+0073.38Uncharacterized protein OS=Cucumis sativus GN=Csa_5G613580 PE=4 SV=1[more]
A0A0A0KJN5_CUCSA4.5e-28067.65Uncharacterized protein OS=Cucumis sativus GN=Csa_5G141040 PE=4 SV=1[more]
A0A0A0KLR4_CUCSA1.0e-24762.14Uncharacterized protein OS=Cucumis sativus GN=Csa_5G187880 PE=4 SV=1[more]
A0A0A0KLY4_CUCSA4.6e-24058.71Uncharacterized protein OS=Cucumis sativus GN=Csa_5G187860 PE=4 SV=1[more]
Match NameE-valueIdentityDescription
AT5G59190.11.0e-18248.65 subtilase family protein[more]
AT3G46850.13.1e-17947.50 Subtilase family protein[more]
AT5G59100.19.9e-17846.15 Subtilisin-like serine endopeptidase family protein[more]
AT5G59090.13.8e-17746.24 subtilase 4.12[more]
AT5G59120.13.8e-17746.05 subtilase 4.13[more]
Match NameE-valueIdentityDescription
gi|700197004|gb|KGN52181.1|0.0e+0081.08hypothetical protein Csa_5G613590 [Cucumis sativus][more]
gi|778708693|ref|XP_004135365.2|0.0e+0081.04PREDICTED: LOW QUALITY PROTEIN: cucumisin-like [Cucumis sativus][more]
gi|659091821|ref|XP_008446751.1|0.0e+0081.29PREDICTED: cucumisin-like [Cucumis melo][more]
gi|700197003|gb|KGN52180.1|0.0e+0073.38hypothetical protein Csa_5G613580 [Cucumis sativus][more]
gi|659074279|ref|XP_008437519.1|9.3e-28768.99PREDICTED: cucumisin-like isoform X1 [Cucumis melo][more]
The following terms have been associated with this gene:
Vocabulary: Biological Process
TermDefinition
GO:0006508proteolysis
Vocabulary: Molecular Function
TermDefinition
GO:0004252serine-type endopeptidase activity
Vocabulary: INTERPRO
TermDefinition
IPR023828Peptidase_S8_Ser-AS
IPR015500Peptidase_S8_subtilisin-rel
IPR010259S8pro/Inhibitor_I9
IPR000209Peptidase_S8/S53_dom
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0006508 proteolysis
cellular_component GO:0005575 cellular_component
molecular_function GO:0004252 serine-type endopeptidase activity

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Cp4.1LG01g14250.1Cp4.1LG01g14250.1mRNA


Analysis Name: InterPro Annotations of Cucurbita pepo
Date Performed: 2017-12-02
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR000209Peptidase S8/S53 domainGENE3DG3DSA:3.40.50.200coord: 129..338
score: 1.5E-77coord: 463..582
score: 1.5
IPR000209Peptidase S8/S53 domainPFAMPF00082Peptidase_S8coord: 130..568
score: 2.5
IPR000209Peptidase S8/S53 domainunknownSSF52743Subtilisin-likecoord: 107..367
score: 4.19E-73coord: 467..586
score: 4.19
IPR010259Peptidase S8 propeptide/proteinase inhibitor I9PFAMPF05922Inhibitor_I9coord: 31..108
score: 1.8
IPR015500Peptidase S8, subtilisin-relatedPRINTSPR00723SUBTILISINcoord: 129..148
score: 8.8E-13coord: 198..211
score: 8.8E-13coord: 520..536
score: 8.8
IPR015500Peptidase S8, subtilisin-relatedPANTHERPTHR10795PROPROTEIN CONVERTASE SUBTILISIN/KEXINcoord: 1..731
score:
IPR023828Peptidase S8, subtilisin, Ser-active sitePROSITEPS00138SUBTILASE_SERcoord: 521..531
scor
NoneNo IPR availablePANTHERPTHR10795:SF348SUBFAMILY NOT NAMEDcoord: 1..731
score:

The following gene(s) are paralogous to this gene:

None