Lsi03G010410 (gene) Bottle gourd (USVL1VR-Ls) | Cucurbit Genomics Database (CuGenDB)

Overview


Name	Lsi03G010410
Type	gene
Organism	Lagenaria siceraria (Bottle gourd (USVL1VR-Ls))
Description	2-oxoglutarate-dependent dioxygenase-related family protein
Location	chr03 : 19999957 .. 20004439 (+)

Genome Viewer

Gene Expression

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: five_prime_UTRCDS

Hold the cursor over a type above to highlight its positions in the sequence below.

TGAGAATATTTTCGTAAAACTTGCAAACCTATTTTACATGTTATTTTGATTTTCTAGTGTTCTTTCATAGTTATTATTTTTATAATAGGACTTTTAGGATGGGCAAATCTTCATAAGCTCCTAGTTAACACATACTAATCTCAAATCAATATTTTTGTCATAACAATCTCAAAATTTCATTTAATTAATTATTGTATCAATAATGATCTCTATAAAAGAGGACAAAAGCAACCACTTTTCTCCTCACACCTTCACCACCAAACATTTAGAAAGAAATTAGAAGGAAAAAAAAAAAGAGTGTAAAAAGGAATGGATAATAATGAATCCTCTCTCAAGCTTCCCCTAATTGATTTCTCCAACCTAGAATTAGGGAGTCCCAAGTGGGAGTTGGCAAAAGCACAAGTGAAGGAAGCACTTGAGGAATTTGGTTGCTTTGAAGCCTCATTTGAGAAAGTGCCTCTTGAGGTTAGAAAGGGTTTGTTTGAAGCACTTGAAGAGCTTTTCAACCTTCCTTTGCAAACCAAATTGAGAAATGTTTCTCAGAAGCCCTTCCATGGCTATGTTGGACAATATCCAATGGCTCCACTTTTTGAAAGCATGGGTGTGGATGATTCCACCATACATGAAAAGGTCCAAAGCTTCACCAATATCTTGTGGCCTCAAGGAAACCCAACCTTTAGGTTTGTATATGTCTACAAATAATGCTATATTCTTTCCTCTTCCTTTTGATTTCATTCCATATTTTGAATTTGTGTTGTTGTTAATTATAAGATTAAGATTAGTTGATAAGTAACATTCTTATTTCTTGTTTGATGTTTCTCTTTGGGGTAGCTGCAAATTATAGCACAGTAAAAGAATTTGCAGATATAGCAAAATTTAGATTCAACTCTCAAAATCTCAGTGATAAACTATATCGCTAATAAGAGTTTATCATCGATAATAGTCTACCCGAGATAAAGTCTATCATTGATAAATTTTGCTATATTTGAAATTCTTTAAAAATATCGCTATACACTTAATTATTATCCCTAAAAGTACTACCCATTGCAATTACCATTCTCTTTTTCAAATTTTTTGAGTTCAACAATGAGTGGAGTAGGAGATTCAAACATCTGACCTCTCTTGATTGAGAGCATATGCCTTAACTAGTTATGAGCTATGCTATTTCTCGTTCAATCAAAAAATAATTGATTTGCGCCCTAGCAACGTTTTTTTAAATAGAGACTTTTATCATCGATTAGTTCAATAATTTTCGATTTTGTTTGACCCTTCTAATGAAGTAGGGTTTTTCTTTTCTTTCCAAAAATCTTTTTGACAGCAAAGTGATAGAATCCTATTCACAACAATTATCAGAACTAGACCAAATGGTGAGAAGGATGGTTTTGGAGAGTTTGGGAATTGAAAAATACTTGGAGGAACATTTGGAGTCAACAAACTATCTTCTTAGAGTGATGAAATACAAAGGCCCTCAAAGCAATGAGACACAACTTGGCCTTCATTCCCACACTGATAAGAACATTGTAACCATTTTATATCAAAATCATGTTGAAGGTTTGCAGGTACAAACCAAAGATGGCAAATGGATTAACTTTCAACCTTCCCCAAACTCCTTTGTTGCAATGATTGGAGATTCCCTCCATGTGAGTAACTTTTTTATTTCATTTGTTTTGTTTCATAGTGATTTTGTTTTTTAGTTTTATGTTCATGTTTGTTTGTTTGTGATTTTTTTGTTTTCTTTTGTGATATTCATTTTTTGAAGGTCATTTTATTAAAAATAAAAACAAATCATAGGCACTTTGTATTTTTTTTTCTTTTTGAAGATTAGTTGGGGATATTTAATTTGTTTTTATCATTATTTTAGTCTTGAACTTTTTTGGTAATATTATTTGCTTTAGTCTTTTGAGAATTGTTTGAATTGTCTTGTGCTTATTGTGTTTCAATCTCTAAATTTATTAACTTATTATTATTAAAAAAGTAATGAATTTTTTAGGGAAGATTTTTAAAAATAGAAAATAAGGAAAACTATTTACACAAAATAGTAAAATTTTTAGATAGTTGTGATAGACCTTGATAGAAGTTTATCACTAATAGATGTTGATAGAAGTCTATCAATGTCCATCTGTATTTTTCCTAGAAAAAATGTCAAACTATTTACAGAAAATAGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAANNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAGCTGGTAGACATTGATAGACTTCTATTAGAATTTATCACTGATAGAATTCTATCATTGATTTCTATCACGGATAGACTTTGATAGACTTCTATCAGTGTCTATCATAACTATCTAAATTTTTTTCTATTTTGTGTAAATAGTTCCCTTATTTTTCTATTTTAAAAATTCCTCTTTTTTCTATCTGTGAACATTTTTTATTTTTTACTGGTATTTTTTTAATAAATAAGTCTAAATGTTATGGTCTTTATGTTTATCTATGCAGTAAGTAGATTTGTCCGTTACAATAAACATAAAGACCATAAAATTTAGACTTATTTATTAAAAAAAATACCAGTAAAAAATGAAAAATTTTCACAGATAGAAAAAAGAGGAATTTTTAAAATAGAAAAATAAGAGAACTATTTACACAAAATATAAAAAAAAATTTGGAGTGATTTTAAAATTGTTAAAATCACTTTTGTCATTTTTAAAATCACTAATAAACACACTTTAATCACTCAAAATTAATTTAATTTTCAATTTTACACTTTTAAATGCAATTTTCAATCATCAAAATTAGTTTTGAAGGATTAAAAATATATTTCACGGTAATTTTGAAAATGACAAAAGTGATTTTAGTTATTTTAAAATCACTTTCAAATATGTCCTAAGGATATGCATTGAGATGATAAATTAATAAAATTTAACCACAATGATTAAACTCAAAGAAAAAAAAACCAATAGAAGAAAGTTTAAAATAAAACATGAAAAGTTGAAGAGAGAGAGAATTCAAACCTTACTTTTCTCTCTTTATAAGAAAAAAGTATTCAAATTTGATTTTAAAAAAAGTAGAAATAATTTTTTTTTTTTAACTACATTATCAAACAAGACCGTAAAATATTATTGAACCCAACACAACGTATTATCATACGAAAATTTTCAACCCATAAGAGTTGCAAAGTGAAAAGCACTATATATTATTTGAACATTTATTAACGTATTTAGATCAAATACTTTCTTACCATTTAGAAAAATTCGATTAAGTGACCGTCAGATTACGACGATGGTTAATGTAGGCATGGACAAATGGAAGATTGCATTCTCCATATCATAGAGTGATGATGAGTGGAGAGGAAGCAAGGTACTCAGCTGGGTTATTCTCAATCCCAAAAGCAGGATATATAATCAAAGCACCAAAAGAACTTATAGATGAACAACATCCTTTGCTCTTCAACCCATTTGATCATGTTGAGTTTCTTCAGTTCTACTACACAGAAGCTGGCCAAAGAGCTCAATCTGCCCTCAAAACTTATTGTGGTGCAACTTGA

mRNA sequence

TGAGAATATTTTCGTAAAACTTGCAAACCTATTTTACATGTTATTTTGATTTTCTAGTGTTCTTTCATAGTTATTATTTTTATAATAGGACTTTTAGGATGGGCAAATCTTCATAAGCTCCTAGTTAACACATACTAATCTCAAATCAATATTTTTGTCATAACAATCTCAAAATTTCATTTAATTAATTATTGTATCAATAATGATCTCTATAAAAGAGGACAAAAGCAACCACTTTTCTCCTCACACCTTCACCACCAAACATTTAGAAAGAAATTAGAAGGAAAAAAAAAAAGAGTGTAAAAAGGAATGGATAATAATGAATCCTCTCTCAAGCTTCCCCTAATTGATTTCTCCAACCTAGAATTAGGGAGTCCCAAGTGGGAGTTGGCAAAAGCACAAGTGAAGGAAGCACTTGAGGAATTTGGTTGCTTTGAAGCCTCATTTGAGAAAGTGCCTCTTGAGGTTAGAAAGGGTTTGTTTGAAGCACTTGAAGAGCTTTTCAACCTTCCTTTGCAAACCAAATTGAGAAATGTTTCTCAGAAGCCCTTCCATGGCTATGTTGGACAATATCCAATGGCTCCACTTTTTGAAAGCATGGGTGTGGATGATTCCACCATACATGAAAAGGTCCAAAGCTTCACCAATATCTTGTGGCCTCAAGGAAACCCAACCTTTAGAGTGATGATGAGTGGAGAGGAAGCAAGGTACTCAGCTGGGTTATTCTCAATCCCAAAAGCAGGATATATAATCAAAGCACCAAAAGAACTTATAGATGAACAACATCCTTTGCTCTTCAACCCATTTGATCATGTTGAGTTTCTTCAGTTCTACTACACAGAAGCTGGCCAAAGAGCTCAATCTGCCCTCAAAACTTATTGTGGTGCAACTTGA

Coding sequence (CDS)

ATGGATAATAATGAATCCTCTCTCAAGCTTCCCCTAATTGATTTCTCCAACCTAGAATTAGGGAGTCCCAAGTGGGAGTTGGCAAAAGCACAAGTGAAGGAAGCACTTGAGGAATTTGGTTGCTTTGAAGCCTCATTTGAGAAAGTGCCTCTTGAGGTTAGAAAGGGTTTGTTTGAAGCACTTGAAGAGCTTTTCAACCTTCCTTTGCAAACCAAATTGAGAAATGTTTCTCAGAAGCCCTTCCATGGCTATGTTGGACAATATCCAATGGCTCCACTTTTTGAAAGCATGGGTGTGGATGATTCCACCATACATGAAAAGGTCCAAAGCTTCACCAATATCTTGTGGCCTCAAGGAAACCCAACCTTTAGAGTGATGATGAGTGGAGAGGAAGCAAGGTACTCAGCTGGGTTATTCTCAATCCCAAAAGCAGGATATATAATCAAAGCACCAAAAGAACTTATAGATGAACAACATCCTTTGCTCTTCAACCCATTTGATCATGTTGAGTTTCTTCAGTTCTACTACACAGAAGCTGGCCAAAGAGCTCAATCTGCCCTCAAAACTTATTGTGGTGCAACTTGA

Protein sequence

MDNNESSLKLPLIDFSNLELGSPKWELAKAQVKEALEEFGCFEASFEKVPLEVRKGLFEALEELFNLPLQTKLRNVSQKPFHGYVGQYPMAPLFESMGVDDSTIHEKVQSFTNILWPQGNPTFRVMMSGEEARYSAGLFSIPKAGYIIKAPKELIDEQHPLLFNPFDHVEFLQFYYTEAGQRAQSALKTYCGAT

Homology

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BLAST of Lsi03G010410 vs. Swiss-Prot
Match: AOP1L_ARATH (Probable 2-oxoglutarate-dependent dioxygenase AOP1.2 OS=Arabidopsis thaliana GN=AOP1.2 PE=2 SV=1)

HSP 1 Score: 127.5 bits (319), Expect = 1.6e-28
Identity = 61/113 (53.98%), Postives = 83/113 (73.45%), Query Frame = 1


Query: 7   SLKLPLIDFS--NLELGSPKWELAKAQVKEALEEFGCFEASFEKVPLEVRKGLFEALEEL 66
           S +LP+IDFS  NL+ GS KW+  KA V +ALE++GCFEA F+K+ +E+ + +FEA+E+L
Sbjct: 11  SFQLPVIDFSDQNLKPGSSKWDEVKADVLKALEDYGCFEAFFDKLSVELNRSVFEAMEDL 70

Query: 67  FNLPLQTKLRNVSQKPFHGYVGQYPMAPLFESMGVDDSTIHEKVQSFTNILWP 118
           F LP+ TK RNVS KPFHGY+       L+ES+G+DD+ + EKV  FT  LWP
Sbjct: 71  FELPIPTKQRNVSSKPFHGYL----CHNLYESLGIDDANVLEKVNDFTQQLWP 119

[Close]

BLAST of Lsi03G010410 vs. Swiss-Prot
Match: AOP1C_ARATH (Probable 2-oxoglutarate-dependent dioxygenase AOP1 OS=Arabidopsis thaliana GN=AOP1 PE=2 SV=1)

HSP 1 Score: 125.6 bits (314), Expect = 6.1e-28
Identity = 60/113 (53.10%), Postives = 83/113 (73.45%), Query Frame = 1


Query: 7   SLKLPLIDFS--NLELGSPKWELAKAQVKEALEEFGCFEASFEKVPLEVRKGLFEALEEL 66
           S +LP+IDFS  NL+ GS KW+   A V +ALE++GCFEASF+K+ +E+ + +FEA+E+L
Sbjct: 11  SFQLPVIDFSDQNLKPGSSKWDEVTADVLKALEDYGCFEASFDKLSVELNRSVFEAMEDL 70

Query: 67  FNLPLQTKLRNVSQKPFHGYVGQYPMAPLFESMGVDDSTIHEKVQSFTNILWP 118
           F LP+ TK RNVS KPFHGY+       L+ES+G++D+ + EKV  FT  LWP
Sbjct: 71  FELPIPTKQRNVSSKPFHGYL----CHNLYESLGINDANVLEKVNDFTQQLWP 119

[Close]

BLAST of Lsi03G010410 vs. Swiss-Prot
Match: AOP1V_ARATH (Probable 2-oxoglutarate-dependent dioxygenase AOP1 (Fragment) OS=Arabidopsis thaliana GN=AOP1 PE=2 SV=1)

HSP 1 Score: 121.7 bits (304), Expect = 8.8e-27
Identity = 59/113 (52.21%), Postives = 82/113 (72.57%), Query Frame = 1


Query: 7   SLKLPLIDFS--NLELGSPKWELAKAQVKEALEEFGCFEASFEKVPLEVRKGLFEALEEL 66
           S +LP+IDFS  NL+ GS KW+   A V +ALE++GCFEASF+K+ +E+ + +FEA+E+L
Sbjct: 4   SFQLPVIDFSDQNLKPGSSKWDEVTADVLKALEDYGCFEASFDKLSVELNRSVFEAMEDL 63

Query: 67  FNLPLQTKLRNVSQKPFHGYVGQYPMAPLFESMGVDDSTIHEKVQSFTNILWP 118
           F LP+ TK RNVS K FHGY+       L+ES+G++D+ + EKV  FT  LWP
Sbjct: 64  FELPIPTKQRNVSSKLFHGYL----CHNLYESLGINDANVLEKVNDFTQQLWP 112

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BLAST of Lsi03G010410 vs. Swiss-Prot
Match: AOP2V_ARATH (2-oxoglutarate-dependent dioxygenase AOP2 OS=Arabidopsis thaliana GN=AOP2 PE=2 SV=1)

HSP 1 Score: 111.7 bits (278), Expect = 9.1e-24
Identity = 54/116 (46.55%), Postives = 82/116 (70.69%), Query Frame = 1


Query: 7   SLKLPLIDFSN--LELGSPKWELAKAQVKEALEEFGCFEASFEKVPLEVRKGLFEALEEL 66
           SL+LPLI+ ++  LE GS KW   ++ V++ALE+FGCFEAS++KV LE+++ + + +EEL
Sbjct: 5   SLQLPLINLADKTLEPGSSKWAEVRSDVRKALEDFGCFEASYDKVSLELQESIMKTMEEL 64

Query: 67  FNLPLQTKLRNVSQKPFHGYVGQYPMAPLFESMGVDDSTIHEKVQSFTNILWPQGN 121
           F LP++TK RNV  KP+ GY+    ++   ES+G+ ++ I E V  FT  LWP G+
Sbjct: 65  FALPVETKQRNVCPKPYVGYLNHNNLS---ESLGISNANILENVNEFTQQLWPDGD 117

[Close]

BLAST of Lsi03G010410 vs. Swiss-Prot
Match: AOP2C_ARATH (Probable inactive 2-oxoglutarate-dependent dioxygenase AOP2 OS=Arabidopsis thaliana GN=AOP2 PE=2 SV=1)

HSP 1 Score: 111.3 bits (277), Expect = 1.2e-23
Identity = 53/116 (45.69%), Postives = 82/116 (70.69%), Query Frame = 1


Query: 7   SLKLPLIDFSN--LELGSPKWELAKAQVKEALEEFGCFEASFEKVPLEVRKGLFEALEEL 66
           SL+LPLI+ ++  LE GS KW   ++ V++ALE+FGCFEAS++KV LE+++ + + +EEL
Sbjct: 5   SLQLPLINLADKTLEPGSSKWAEVRSDVRKALEDFGCFEASYDKVSLELQESIMKTMEEL 64

Query: 67  FNLPLQTKLRNVSQKPFHGYVGQYPMAPLFESMGVDDSTIHEKVQSFTNILWPQGN 121
           F LP++TK RNV  KP+ GY+    ++   ES+G+ ++ I E +  FT  LWP G+
Sbjct: 65  FALPVETKQRNVCPKPYVGYLNHNNLS---ESLGISNANILENINEFTQQLWPHGD 117

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BLAST of Lsi03G010410 vs. TrEMBL
Match: A0A0A0KT26_CUCSA (Uncharacterized protein OS=Cucumis sativus GN=Csa_4G001530 PE=4 SV=1)

HSP 1 Score: 234.6 bits (597), Expect = 1.0e-58
Identity = 112/125 (89.60%), Postives = 119/125 (95.20%), Query Frame = 1


Query: 3   NNESSLKLPLIDFSNLELGSPKWELAKAQVKEALEEFGCFEASFEKVPLEVRKGLFEALE 62
           ++ESSLKLPLIDFSNLE G PKWELAKAQVKEALEEFGCFEASF+KVP+EVRKGLFEALE
Sbjct: 2   DSESSLKLPLIDFSNLESGGPKWELAKAQVKEALEEFGCFEASFDKVPVEVRKGLFEALE 61

Query: 63  ELFNLPLQTKLRNVSQKPFHGYVGQYPMAPLFESMGVDDSTIHEKVQSFTNILWPQGNPT 122
           ELFNLPL+TKLRNVSQKPFHGYVGQYPMAPLFESMGVDDSTI +KVQ FTNILWPQGNPT
Sbjct: 62  ELFNLPLETKLRNVSQKPFHGYVGQYPMAPLFESMGVDDSTIPQKVQDFTNILWPQGNPT 121

Query: 123 FRVMM 128
           F  +M
Sbjct: 122 FSKVM 126

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BLAST of Lsi03G010410 vs. TrEMBL
Match: A0A0B2REW5_GLYSO (Uncharacterized protein OS=Glycine soja GN=glysoja_025883 PE=4 SV=1)

HSP 1 Score: 195.7 bits (496), Expect = 5.3e-47
Identity = 99/190 (52.11%), Postives = 134/190 (70.53%), Query Frame = 1


Query: 4   NESSLKLPLIDFS--NLELGSPKWELAKAQVKEALEEFGCFEASFEKVPLEVRKGLFEAL 63
           +E  LKLP IDFS  +LE    KWEL K+QV +AL E+GCFEA F+KVPL++RK +F  +
Sbjct: 3   SEPELKLPTIDFSIEDLEFNVAKWELVKSQVHKALVEYGCFEALFDKVPLDLRKAIFLQV 62

Query: 64  EELFNLPLQTKLRNVSQKPFHGYVGQYPMAPLFESMGVDDSTIHEKVQSFTNILWPQGNP 123
           EE+F+LPLQTK R VS +P+HGYVG      L+E+M +DD   H  +Q++TN       P
Sbjct: 63  EEMFDLPLQTKQRVVSSRPYHGYVGP---LQLYENMVIDDVDNHNVLQAWTN--GRVHTP 122

Query: 124 TFRVMMSGEEARYSAGLFSIPKAGYIIKAPKELIDEQHPLLFNPFDHVEFLQFYYTEAGQ 183
             RVMMSG E R++ GLF++PK G+IIKAP+EL+ E+HPLLF PF   EF++F +  + +
Sbjct: 123 NHRVMMSGNETRFTIGLFTVPKPGFIIKAPEELVTEEHPLLFKPFVQSEFMKFLH--SSE 182

Query: 184 RAQSALKTYC 192
             ++ALK YC
Sbjct: 183 STKNALKVYC 185

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BLAST of Lsi03G010410 vs. TrEMBL
Match: K7KZF3_SOYBN (Uncharacterized protein OS=Glycine max GN=GLYMA_07G033700 PE=3 SV=1)

HSP 1 Score: 181.0 bits (458), Expect = 1.4e-42
Identity = 80/122 (65.57%), Postives = 105/122 (86.07%), Query Frame = 1


Query: 4   NESSLKLPLIDFSNLEL--GSPKWELAKAQVKEALEEFGCFEASFEKVPLEVRKGLFEAL 63
           +E++LKLP+IDF+NL+L   +P WE  K+QV +AL ++GCFEA FEKVPLE+RK +F AL
Sbjct: 3   SEATLKLPVIDFTNLKLEANNPNWEAIKSQVHKALVDYGCFEAIFEKVPLELRKAIFAAL 62

Query: 64  EELFNLPLQTKLRNVSQKPFHGYVGQYPMAPLFESMGVDDSTIHEKVQSFTNILWPQGNP 123
           +ELF+LPLQTK+ NVS+KP+HGYVGQYPM PLFESMG+DD+ ++E V+S TNI+WP GNP
Sbjct: 63  QELFDLPLQTKILNVSKKPYHGYVGQYPMVPLFESMGIDDANVYENVESMTNIMWPHGNP 122

[Close]

BLAST of Lsi03G010410 vs. TrEMBL
Match: A0A0B2NW45_GLYSO (Gibberellin 20 oxidase 1 OS=Glycine soja GN=glysoja_009567 PE=3 SV=1)

HSP 1 Score: 181.0 bits (458), Expect = 1.4e-42
Identity = 80/122 (65.57%), Postives = 105/122 (86.07%), Query Frame = 1


Query: 4   NESSLKLPLIDFSNLEL--GSPKWELAKAQVKEALEEFGCFEASFEKVPLEVRKGLFEAL 63
           +E++LKLP+IDF+NL+L   +P WE  K+QV +AL ++GCFEA FEKVPLE+RK +F AL
Sbjct: 3   SEATLKLPVIDFTNLKLEANNPNWEAIKSQVHKALVDYGCFEAIFEKVPLELRKAIFAAL 62

Query: 64  EELFNLPLQTKLRNVSQKPFHGYVGQYPMAPLFESMGVDDSTIHEKVQSFTNILWPQGNP 123
           +ELF+LPLQTK+ NVS+KP+HGYVGQYPM PLFESMG+DD+ ++E V+S TNI+WP GNP
Sbjct: 63  QELFDLPLQTKILNVSKKPYHGYVGQYPMVPLFESMGIDDANVYENVESMTNIMWPHGNP 122

[Close]

BLAST of Lsi03G010410 vs. TrEMBL
Match: A0A068ULG4_COFCA (Uncharacterized protein OS=Coffea canephora GN=GSCOC_T00027361001 PE=3 SV=1)

HSP 1 Score: 180.6 bits (457), Expect = 1.8e-42
Identity = 81/116 (69.83%), Postives = 103/116 (88.79%), Query Frame = 1


Query: 10  LPLIDFSN--LELGSPKWELAKAQVKEALEEFGCFEASFEKVPLEVRKGLFEALEELFNL 69
           LP IDFSN  L+ G+P+W+L K QV++ALEE+GCFEA F+K+PLE++K LF+ALEELF+L
Sbjct: 8   LPTIDFSNPGLKPGAPEWDLVKDQVRKALEEYGCFEALFDKIPLELQKSLFDALEELFDL 67

Query: 70  PLQTKLRNVSQKPFHGYVGQYPMAPLFESMGVDDSTIHEKVQSFTNILWPQGNPTF 124
           PLQTK+RN S+KPFHGYVGQYPM PL+ESMG+DD+TI EKV+SFT++LWP+GN  F
Sbjct: 68  PLQTKVRNSSKKPFHGYVGQYPMVPLYESMGIDDATISEKVESFTDLLWPEGNSNF 123

[Close]

BLAST of Lsi03G010410 vs. TAIR10
Match: AT1G52820.1 (AT1G52820.1 2-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily protein)

HSP 1 Score: 172.6 bits (436), Expect = 2.4e-43
Identity = 79/118 (66.95%), Postives = 100/118 (84.75%), Query Frame = 1


Query: 8   LKLPLIDFSNLEL--GSPKWELAKAQVKEALEEFGCFEASFEKVPLEVRKGLFEALEELF 67
           L+LP+IDFSN  L  G P+W+L +A V++AL+++G FEASF+++P E+RK +F ALEELF
Sbjct: 10  LRLPVIDFSNKNLKPGEPEWDLTRADVQKALQDYGYFEASFDRIPFELRKSVFGALEELF 69

Query: 68  NLPLQTKLRNVSQKPFHGYVGQYPMAPLFESMGVDDSTIHEKVQSFTNILWPQGNPTF 124
           +LPLQTKLRNVS+KPFHGYVGQYPM PL+ESMG+DDS I EKV +FT  LWPQGN +F
Sbjct: 70  DLPLQTKLRNVSKKPFHGYVGQYPMVPLYESMGIDDSDIAEKVDAFTEKLWPQGNISF 127

[Close]

BLAST of Lsi03G010410 vs. TAIR10
Match: AT1G52810.1 (AT1G52810.1 2-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily protein)

HSP 1 Score: 139.4 bits (350), Expect = 2.3e-33
Identity = 66/119 (55.46%), Postives = 91/119 (76.47%), Query Frame = 1


Query: 7   SLKLPLIDFSNLEL--GSPKWELAKAQVKEALEEFGCFEASFEKVPLEVRKGLFEALEEL 66
           +L+LP+IDF++ +L  G+ +W+  +A V+ ALEE+GCFEA F+KVPLE+RK +F+A EE+
Sbjct: 9   ALQLPVIDFTSRDLKPGTIQWDSVRADVRRALEEYGCFEALFDKVPLELRKAVFDASEEV 68

Query: 67  FNLPLQTKLRNVSQKPFHGYVGQYPMAPLFESMGVDDSTIHEKVQSFTNILWPQGNPTF 124
           F LPL+TK R VS++ + GYVGQ P  PLFE MGVD +   +KV +FT+ LWPQGN  F
Sbjct: 69  FQLPLETKKRVVSKRKYRGYVGQIPTLPLFEVMGVDFAENPDKVNAFTHKLWPQGNNNF 127

[Close]

BLAST of Lsi03G010410 vs. TAIR10
Match: AT4G03070.1 (AT4G03070.1 2-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily protein)

HSP 1 Score: 125.6 bits (314), Expect = 3.4e-29
Identity = 60/113 (53.10%), Postives = 83/113 (73.45%), Query Frame = 1


Query: 7   SLKLPLIDFS--NLELGSPKWELAKAQVKEALEEFGCFEASFEKVPLEVRKGLFEALEEL 66
           S +LP+IDFS  NL+ GS KW+   A V +ALE++GCFEASF+K+ +E+ + +FEA+E+L
Sbjct: 11  SFQLPVIDFSDQNLKPGSSKWDEVTADVLKALEDYGCFEASFDKLSVELNRSVFEAMEDL 70

Query: 67  FNLPLQTKLRNVSQKPFHGYVGQYPMAPLFESMGVDDSTIHEKVQSFTNILWP 118
           F LP+ TK RNVS KPFHGY+       L+ES+G++D+ + EKV  FT  LWP
Sbjct: 71  FELPIPTKQRNVSSKPFHGYL----CHNLYESLGINDANVLEKVNDFTQQLWP 119

[Close]

BLAST of Lsi03G010410 vs. TAIR10
Match: AT1G52790.1 (AT1G52790.1 2-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily protein)

HSP 1 Score: 117.1 bits (292), Expect = 1.2e-26
Identity = 55/120 (45.83%), Postives = 81/120 (67.50%), Query Frame = 1


Query: 7   SLKLPLIDFSNLEL--GSPKWELAKAQVKEALEEFGCFEASF-EKVPLEVRKGLFEALEE 66
           S K+P +DFS  +L  G+  WE  +  +++ALEE+GCF     +K PL++   +F +L +
Sbjct: 2   SPKIPTLDFSREDLKPGTKYWESTRENIRQALEEYGCFIIDLKDKTPLDLLDRVFGSLVD 61

Query: 67  LFNLPLQTKLRNVSQKPFHGYVGQYPMAPLFESMGVDDSTIHEKVQSFTNILWPQGNPTF 124
           LF+LP QTK++N   KP +GYVGQ P  PL ES+G+D++T  E  +SFT ++WPQGN  F
Sbjct: 62  LFDLPTQTKMKNKYDKPLNGYVGQIPALPLHESLGIDNATSLEATRSFTGLMWPQGNEHF 121

[Close]

BLAST of Lsi03G010410 vs. TAIR10
Match: AT1G52800.1 (AT1G52800.1 2-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily protein)

HSP 1 Score: 115.2 bits (287), Expect = 4.6e-26
Identity = 53/118 (44.92%), Postives = 78/118 (66.10%), Query Frame = 1


Query: 9   KLPLIDFSN---LELGSPKWELAKAQVKEALEEFGCFEASFEKVPLEVRKGLFEALEELF 68
           K+P++D ++   L+  +  W     +  EALEE+GCF A ++ V  ++   +F A EELF
Sbjct: 9   KVPILDLTSQQELKPNTSTWRSVSREACEALEEYGCFLAVYDGVTQQLDDSIFAAAEELF 68

Query: 69  NLPLQTKLRNVSQKPFHGYVGQYPMAPLFESMGVDDSTIHEKVQSFTNILWPQGNPTF 124
           +LP +TK +NV++KP+HGYVGQ P+ PL E +GVD  T  E  Q FT+++WPQGN  F
Sbjct: 69  DLPTETKKKNVNEKPYHGYVGQMPVIPLHEGLGVDYVTNKEIAQRFTHLMWPQGNDRF 126

[Close]

BLAST of Lsi03G010410 vs. NCBI nr
Match: gi|449469106|ref|XP_004152262.1| (PREDICTED: probable 2-oxoglutarate-dependent dioxygenase AOP1 [Cucumis sativus])

HSP 1 Score: 234.6 bits (597), Expect = 1.5e-58
Identity = 112/125 (89.60%), Postives = 119/125 (95.20%), Query Frame = 1


Query: 3   NNESSLKLPLIDFSNLELGSPKWELAKAQVKEALEEFGCFEASFEKVPLEVRKGLFEALE 62
           ++ESSLKLPLIDFSNLE G PKWELAKAQVKEALEEFGCFEASF+KVP+EVRKGLFEALE
Sbjct: 2   DSESSLKLPLIDFSNLESGGPKWELAKAQVKEALEEFGCFEASFDKVPVEVRKGLFEALE 61

Query: 63  ELFNLPLQTKLRNVSQKPFHGYVGQYPMAPLFESMGVDDSTIHEKVQSFTNILWPQGNPT 122
           ELFNLPL+TKLRNVSQKPFHGYVGQYPMAPLFESMGVDDSTI +KVQ FTNILWPQGNPT
Sbjct: 62  ELFNLPLETKLRNVSQKPFHGYVGQYPMAPLFESMGVDDSTIPQKVQDFTNILWPQGNPT 121

Query: 123 FRVMM 128
           F  +M
Sbjct: 122 FSKVM 126

[Close]

BLAST of Lsi03G010410 vs. NCBI nr
Match: gi|700197627|gb|KGN52785.1| (hypothetical protein Csa_4G001530 [Cucumis sativus])

HSP 1 Score: 234.6 bits (597), Expect = 1.5e-58
Identity = 112/125 (89.60%), Postives = 119/125 (95.20%), Query Frame = 1


Query: 3   NNESSLKLPLIDFSNLELGSPKWELAKAQVKEALEEFGCFEASFEKVPLEVRKGLFEALE 62
           ++ESSLKLPLIDFSNLE G PKWELAKAQVKEALEEFGCFEASF+KVP+EVRKGLFEALE
Sbjct: 2   DSESSLKLPLIDFSNLESGGPKWELAKAQVKEALEEFGCFEASFDKVPVEVRKGLFEALE 61

Query: 63  ELFNLPLQTKLRNVSQKPFHGYVGQYPMAPLFESMGVDDSTIHEKVQSFTNILWPQGNPT 122
           ELFNLPL+TKLRNVSQKPFHGYVGQYPMAPLFESMGVDDSTI +KVQ FTNILWPQGNPT
Sbjct: 62  ELFNLPLETKLRNVSQKPFHGYVGQYPMAPLFESMGVDDSTIPQKVQDFTNILWPQGNPT 121

Query: 123 FRVMM 128
           F  +M
Sbjct: 122 FSKVM 126

[Close]

BLAST of Lsi03G010410 vs. NCBI nr
Match: gi|659108754|ref|XP_008454372.1| (PREDICTED: probable 2-oxoglutarate-dependent dioxygenase AOP1 [Cucumis melo])

HSP 1 Score: 233.0 bits (593), Expect = 4.3e-58
Identity = 111/121 (91.74%), Postives = 116/121 (95.87%), Query Frame = 1


Query: 3   NNESSLKLPLIDFSNLELGSPKWELAKAQVKEALEEFGCFEASFEKVPLEVRKGLFEALE 62
           + ESSLKLPLIDFSNLE G PKWELAKAQVKEALEEFGCFEASF+KVP+EVRKGLFEALE
Sbjct: 2   DGESSLKLPLIDFSNLESGGPKWELAKAQVKEALEEFGCFEASFDKVPIEVRKGLFEALE 61

Query: 63  ELFNLPLQTKLRNVSQKPFHGYVGQYPMAPLFESMGVDDSTIHEKVQSFTNILWPQGNPT 122
           ELFNLPL+TKLRNVSQKPFHGYVGQYPMAPLFESMGVDDSTI +KVQ FTNILWPQGNPT
Sbjct: 62  ELFNLPLETKLRNVSQKPFHGYVGQYPMAPLFESMGVDDSTIPQKVQDFTNILWPQGNPT 121

Query: 123 F 124
           F
Sbjct: 122 F 122

[Close]

BLAST of Lsi03G010410 vs. NCBI nr
Match: gi|645244959|ref|XP_008228655.1| (PREDICTED: probable 2-oxoglutarate-dependent dioxygenase AOP1.2 [Prunus mume])

HSP 1 Score: 224.2 bits (570), Expect = 2.0e-55
Identity = 111/197 (56.35%), Postives = 141/197 (71.57%), Query Frame = 1


Query: 1   MDNNESSLKLPLIDFSN--LELGSPKWELAKAQVKEALEEFGCFEASFEKVPLEVRKGLF 60
           M ++E+ LKLP+ DFS     LGS +WE  + QV  ALEE+GCFEA   KVP  +RK +F
Sbjct: 1   MGSSETPLKLPITDFSKQAFTLGSKEWETVRTQVHNALEEYGCFEALLYKVPQHIRKSIF 60

Query: 61  EALEELFNLPLQTKLRNVSQKPFHGYVGQYPMAPLFESMGVDDSTIHEKVQSFTNILWPQ 120
           +++EELFNLPL+TK++NVS+K             ++ES+GVDD  ++E+        W  
Sbjct: 61  QSMEELFNLPLKTKMQNVSKKTLPWLCWPPHGGTIYESIGVDDVNVYEQA-------WTN 120

Query: 121 G---NPTFRVMMSGEEARYSAGLFSIPKAGYIIKAPKELIDEQHPLLFNPFDHVEFLQFY 180
           G   +P  R+MMSG EARYS GLFSIPK GYIIKAP+E++DE+HPLLF PFDHV+FL FY
Sbjct: 121 GRLHSPYHRLMMSGNEARYSTGLFSIPKEGYIIKAPEEVVDEEHPLLFKPFDHVQFLGFY 180

Query: 181 YTEAGQRAQSALKTYCG 193
           Y+EAGQRA SALKTYCG
Sbjct: 181 YSEAGQRAHSALKTYCG 190

[Close]

BLAST of Lsi03G010410 vs. NCBI nr
Match: gi|734397951|gb|KHN30392.1| (hypothetical protein glysoja_025883 [Glycine soja])

HSP 1 Score: 195.7 bits (496), Expect = 7.7e-47
Identity = 99/190 (52.11%), Postives = 134/190 (70.53%), Query Frame = 1


Query: 4   NESSLKLPLIDFS--NLELGSPKWELAKAQVKEALEEFGCFEASFEKVPLEVRKGLFEAL 63
           +E  LKLP IDFS  +LE    KWEL K+QV +AL E+GCFEA F+KVPL++RK +F  +
Sbjct: 3   SEPELKLPTIDFSIEDLEFNVAKWELVKSQVHKALVEYGCFEALFDKVPLDLRKAIFLQV 62

Query: 64  EELFNLPLQTKLRNVSQKPFHGYVGQYPMAPLFESMGVDDSTIHEKVQSFTNILWPQGNP 123
           EE+F+LPLQTK R VS +P+HGYVG      L+E+M +DD   H  +Q++TN       P
Sbjct: 63  EEMFDLPLQTKQRVVSSRPYHGYVGP---LQLYENMVIDDVDNHNVLQAWTN--GRVHTP 122

Query: 124 TFRVMMSGEEARYSAGLFSIPKAGYIIKAPKELIDEQHPLLFNPFDHVEFLQFYYTEAGQ 183
             RVMMSG E R++ GLF++PK G+IIKAP+EL+ E+HPLLF PF   EF++F +  + +
Sbjct: 123 NHRVMMSGNETRFTIGLFTVPKPGFIIKAPEELVTEEHPLLFKPFVQSEFMKFLH--SSE 182

Query: 184 RAQSALKTYC 192
             ++ALK YC
Sbjct: 183 STKNALKVYC 185

The following BLAST results are available for this feature:

BLAST of Lsi03G010410 vs. Swiss-Prot


Match Name	E-value	Identity	Description
AOP1L_ARATH	1.6e-28	53.98	Probable 2-oxoglutarate-dependent dioxygenase AOP1.2 OS=Arabidopsis thaliana GN=...	[more]
AOP1C_ARATH	6.1e-28	53.10	Probable 2-oxoglutarate-dependent dioxygenase AOP1 OS=Arabidopsis thaliana GN=AO...	[more]
AOP1V_ARATH	8.8e-27	52.21	Probable 2-oxoglutarate-dependent dioxygenase AOP1 (Fragment) OS=Arabidopsis tha...	[more]
AOP2V_ARATH	9.1e-24	46.55	2-oxoglutarate-dependent dioxygenase AOP2 OS=Arabidopsis thaliana GN=AOP2 PE=2 S...	[more]
AOP2C_ARATH	1.2e-23	45.69	Probable inactive 2-oxoglutarate-dependent dioxygenase AOP2 OS=Arabidopsis thali...	[more]

BLAST of Lsi03G010410 vs. TrEMBL


Match Name	E-value	Identity	Description
A0A0A0KT26_CUCSA	1.0e-58	89.60	Uncharacterized protein OS=Cucumis sativus GN=Csa_4G001530 PE=4 SV=1	[more]
A0A0B2REW5_GLYSO	5.3e-47	52.11	Uncharacterized protein OS=Glycine soja GN=glysoja_025883 PE=4 SV=1	[more]
K7KZF3_SOYBN	1.4e-42	65.57	Uncharacterized protein OS=Glycine max GN=GLYMA_07G033700 PE=3 SV=1	[more]
A0A0B2NW45_GLYSO	1.4e-42	65.57	Gibberellin 20 oxidase 1 OS=Glycine soja GN=glysoja_009567 PE=3 SV=1	[more]
A0A068ULG4_COFCA	1.8e-42	69.83	Uncharacterized protein OS=Coffea canephora GN=GSCOC_T00027361001 PE=3 SV=1	[more]

BLAST of Lsi03G010410 vs. TAIR10


Match Name	E-value	Identity	Description
AT1G52820.1	2.4e-43	66.95	2-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily prot...	[more]
AT1G52810.1	2.3e-33	55.46	2-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily prot...	[more]
AT4G03070.1	3.4e-29	53.10	2-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily prot...	[more]
AT1G52790.1	1.2e-26	45.83	2-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily prot...	[more]
AT1G52800.1	4.6e-26	44.92	2-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily prot...	[more]

BLAST of Lsi03G010410 vs. NCBI nr


Match Name	E-value	Identity	Description
gi\|449469106\|ref\|XP_004152262.1\|	1.5e-58	89.60	PREDICTED: probable 2-oxoglutarate-dependent dioxygenase AOP1 [Cucumis sativus]	[more]
gi\|700197627\|gb\|KGN52785.1\|	1.5e-58	89.60	hypothetical protein Csa_4G001530 [Cucumis sativus]	[more]
gi\|659108754\|ref\|XP_008454372.1\|	4.3e-58	91.74	PREDICTED: probable 2-oxoglutarate-dependent dioxygenase AOP1 [Cucumis melo]	[more]
gi\|645244959\|ref\|XP_008228655.1\|	2.0e-55	56.35	PREDICTED: probable 2-oxoglutarate-dependent dioxygenase AOP1.2 [Prunus mume]	[more]
gi\|734397951\|gb\|KHN30392.1\|	7.7e-47	52.11	hypothetical protein glysoja_025883 [Glycine soja]	[more]

Annotated Terms

The following terms have been associated with this gene:

Vocabulary: **INTERPRO**
Term	Definition
IPR027443	IPNS-like
IPR026992	DIOX_N

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0055114	oxidation-reduction process
biological_process	GO:0016102	diterpenoid biosynthetic process
cellular_component	GO:0005575	cellular_component
molecular_function	GO:0051213	dioxygenase activity
molecular_function	GO:0005506	iron ion binding
molecular_function	GO:0016706	oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen, 2-oxoglutarate as one donor, and incorporation of one atom each of oxygen into both donors
molecular_function	GO:0016707	gibberellin 3-beta-dioxygenase activity
molecular_function	GO:0046872	metal ion binding
molecular_function	GO:0016491	oxidoreductase activity

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Lsi03G010410.1	Lsi03G010410.1	mRNA

InterPro

Analysis Name: InterPro Annotations of Lagenaria siceraria
Date Performed: 2017-09-18


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR026992	Non-haem dioxygenase N-terminal domain	PFAM	PF14226	DIOX_N	coord: 10..86 score: 2.2
IPR027443	Isopenicillin N synthase-like	GENE3D	G3DSA:2.60.120.330		coord: 126..181 score: 4.2E-8coord: 9..125 score: 2.5
None	No IPR available	PANTHER	PTHR10209	OXIDOREDUCTASE, 2OG-FE II OXYGENASE FAMILY PROTEIN	coord: 1..185 score: 6.3
None	No IPR available	PANTHER	PTHR10209:SF211	2-OXOGLUTARATE (2OG) AND FE(II)-DEPENDENT OXYGENASE SUPERFAMILY PROTEIN-RELATED	coord: 1..185 score: 6.3
None	No IPR available	unknown	SSF51197	Clavaminate synthase-like	coord: 5..179 score: 5.89

Synteny

The following gene(s) are orthologous to this gene:


Gene	Orthologue	Organism	Block
Lsi03G010410	Csa4G001530	Cucumber (Chinese Long) v2	culsiB275
Lsi03G010410	CSPI04G00590	Wild cucumber (PI 183967)	cpilsiB274
Lsi03G010410	Cucsa.097340	Cucumber (Gy14) v1	cgylsiB111
Lsi03G010410	CmaCh14G008860	Cucurbita maxima (Rimu)	cmalsiB240
Lsi03G010410	CmoCh14G009060	Cucurbita moschata (Rifu)	cmolsiB228
Lsi03G010410	Cla001298	Watermelon (97103) v1	lsiwmB240
Lsi03G010410	MELO3C017981	Melon (DHL92) v3.5.1	lsimeB269
Lsi03G010410	ClCG10G010620	Watermelon (Charleston Gray)	lsiwcgB219
Lsi03G010410	ClCG10G010780	Watermelon (Charleston Gray)	lsiwcgB221
Lsi03G010410	MELO3C017981.2	Melon (DHL92) v3.6.1	lsimedB282
Lsi03G010410	Cla97C10G193540	Watermelon (97103) v2	lsiwmbB218
Lsi03G010410	Bhi11G000977	Wax gourd	lsiwgoB292
Lsi03G010410	Cp4.1LG03g05520	Cucurbita pepo (Zucchini)	cpelsiB500
Lsi03G010410	CsGy4G000510	Cucumber (Gy14) v2	cgyblsiB257
Lsi03G010410	CsaV3_4G000540	Cucumber (Chinese Long) v3	cuclsiB296

The following gene(s) are paralogous to this gene:

None

The following block(s) are covering this gene:


Gene	Organism	Block
Lsi03G010410	Cucurbita maxima (Rimu)	cmalsiB115
Lsi03G010410	Cucurbita maxima (Rimu)	cmalsiB422
Lsi03G010410	Cucurbita moschata (Rifu)	cmolsiB108
Lsi03G010410	Cucurbita moschata (Rifu)	cmolsiB409
Lsi03G010410	Silver-seed gourd	carlsiB699