CSPI04G00590 (gene) Wild cucumber (PI 183967)

Overview
NameCSPI04G00590
Typegene
OrganismCucumis sativus (Wild cucumber (PI 183967))
Description2-oxoglutarate-dependent dioxygenase-related family protein
LocationChr4 : 302176 .. 303915 (+)
Genome Viewer
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: five_prime_UTRCDSthree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
CCCACCAAACACTTAGAAAAAAGAAAAAGGAATTAGAAGGAAAAATAAAAGAGTGTAAAAGGGAATGGATAGTGAATCCTCTCTTAAGCTTCCCCTAATTGATTTCTCCAATTTAGAATCAGGGGGTCCTAAGTGGGAGTTGGCAAAAGCACAAGTGAAGGAAGCACTTGAGGAATTTGGTTGCTTTGAAGCCTCATTTGACAAAGTGCCTGTTGAGGTCAGAAAGGGTTTGTTTGAAGCACTTGAAGAGCTTTTCAACCTTCCTTTGGAAACCAAATTGAGAAATGTTTCTCAAAAGCCCTTCCATGGCTATGTTGGACAGTACCCAATGGCTCCACTTTTTGAAAGTATGGGTGTGGATGATTCCACCATACCTCAAAAGGTCCAAGACTTCACCAATATCTTGTGGCCTCAAGGAAACCCAACCTTTAGGTTTGAATATGTTCTACAAATAATGCTATCTTATTAATTATTTCCCTCTTCTCCTTCTCATTTTTATTTCATACTTTGAATCTATATTATACGTTATTACTTGTTTATTTTTTCTTACCTTTATCGATCGAAAATATGTCTTAACTAGTTGTGATCTTAATTTTGATGACTAATTAATGATAAGAGTCTCTGTCTTGAACCTTCTAATTAGGGTTTTTTTTCCTTTTCTTACAAATCTTGTTGAGTACAGCAAAGTGATGGAAACCTATTCACAACAATTAGCAGAACTAGACGAGATGGTGAGAAGAATGGTTTTGGAGAGTTTGGGAGTTGAAAAGTATTTGGAGGAACATTTGGAGTCAACAAACTATCTTCTTAGAGTGATGAAATACAAAGGGACTGAAAGCAATTATGAAACAAAAATTGGGCTTCATTCCCACACTGATAAGAACATTGTAACCATTTTATATCAAAACCATGTTCAAGGTTTACAGGTAAAAACCAAAGATGGGAAATGGATTAACTTTCAACCTTCCCCAGACTCCTTTGTTGCAATGATTGGAGATTCCTTCCATGTGAGTAACCTTATATTACTTCATTTTTGTTTTCTGATATATATTCATTTTATTAAAAAAAAAATGGTATACTTCAGAAAAAACTTTGTATTATTTATTACTATTTTTTTGTTTTCGAAGTTTGGTTGGGGATTTTTAATTTAATTTAATTATTGTTATTATCTAAACATAACTTTTATGCATGGTAATTAATTAATTGCTTTAGTCCTCCTCAACTTTGTAGTTTTGGTAAAAGGTGTTCCTAACTTTCGAATATCTCAAAAATTTGGAAAAATTTAATAAACACCCTTTACACTTAGAAAAGTTAAAAGATGATATATGATATAGATATATGCATGTAGGCATGGACAAATGGGAGATTGCATTCTCCATATCATAGAGTGATGATGAGTGGAGAAGAAGTGAGGTACTCAGCTGGGTTATTCTCAATCCCAAAGGCAGGCTACATAGTGAAAGCACCAGAAGAGCTTGTAGATGAACAACATCCTTTGCTCTTCAATCCATTTGATCATGTTCAATTTCTTCAGTTCTACTATACAGAAGCTGGCCAAAAAGCTCAATCTGCCCTCAAAACTTATTGTGGTGCAACTTAGAAACATTGATATATATATATATATCTTTCTTGCTTCAACAATAATATTTTCTTTTTTGTATTTGGACTACATATATAATATTATTACGTCTTTGATGCTTGTGTATCATTTTCTTTAGTTTAATAATATCCATCCAAGAC

mRNA sequence

ATGGATAGTGAATCCTCTCTTAAGCTTCCCCTAATTGATTTCTCCAATTTAGAATCAGGGGGTCCTAAGTGGGAGTTGGCAAAAGCACAAGTGAAGGAAGCACTTGAGGAATTTGGTTGCTTTGAAGCCTCATTTGACAAAGTGCCTGTTGAGGTCAGAAAGGGTTTGTTTGAAGCACTTGAAGAGCTTTTCAACCTTCCTTTGGAAACCAAATTGAGAAATGTTTCTCAAAAGCCCTTCCATGGCTATGTTGGACAGTACCCAATGGCTCCACTTTTTGAAAGTATGGGTGTGGATGATTCCACCATACCTCAAAAGGTCCAAGACTTCACCAATATCTTGTGGCCTCAAGGAAACCCAACCTTTAGCAAAGTGATGGAAACCTATTCACAACAATTAGCAGAACTAGACGAGATGGTGAGAAGAATGGTTTTGGAGAGTTTGGGAGTTGAAAAGTATTTGGAGGAACATTTGGAGTCAACAAACTATCTTCTTAGAGTGATGAAATACAAAGGGACTGAAAGCAATTATGAAACAAAAATTGGGCTTCATTCCCACACTGATAAGAACATTGTAACCATTTTATATCAAAACCATGTTCAAGGTTTACAGGTAAAAACCAAAGATGGGAAATGGATTAACTTTCAACCTTCCCCAGACTCCTTTGTTGCAATGATTGGAGATTCCTTCCATGCATGGACAAATGGGAGATTGCATTCTCCATATCATAGAGTGATGATGAGTGGAGAAGAAGTGAGGTACTCAGCTGGGTTATTCTCAATCCCAAAGGCAGGCTACATAGTGAAAGCACCAGAAGAGCTTGTAGATGAACAACATCCTTTGCTCTTCAATCCATTTGATCATGTTCAATTTCTTCAGTTCTACTATACAGAAGCTGGCCAAAAAGCTCAATCTGCCCTCAAAACTTATTGTGGTGCAACTTAG

Coding sequence (CDS)

ATGGATAGTGAATCCTCTCTTAAGCTTCCCCTAATTGATTTCTCCAATTTAGAATCAGGGGGTCCTAAGTGGGAGTTGGCAAAAGCACAAGTGAAGGAAGCACTTGAGGAATTTGGTTGCTTTGAAGCCTCATTTGACAAAGTGCCTGTTGAGGTCAGAAAGGGTTTGTTTGAAGCACTTGAAGAGCTTTTCAACCTTCCTTTGGAAACCAAATTGAGAAATGTTTCTCAAAAGCCCTTCCATGGCTATGTTGGACAGTACCCAATGGCTCCACTTTTTGAAAGTATGGGTGTGGATGATTCCACCATACCTCAAAAGGTCCAAGACTTCACCAATATCTTGTGGCCTCAAGGAAACCCAACCTTTAGCAAAGTGATGGAAACCTATTCACAACAATTAGCAGAACTAGACGAGATGGTGAGAAGAATGGTTTTGGAGAGTTTGGGAGTTGAAAAGTATTTGGAGGAACATTTGGAGTCAACAAACTATCTTCTTAGAGTGATGAAATACAAAGGGACTGAAAGCAATTATGAAACAAAAATTGGGCTTCATTCCCACACTGATAAGAACATTGTAACCATTTTATATCAAAACCATGTTCAAGGTTTACAGGTAAAAACCAAAGATGGGAAATGGATTAACTTTCAACCTTCCCCAGACTCCTTTGTTGCAATGATTGGAGATTCCTTCCATGCATGGACAAATGGGAGATTGCATTCTCCATATCATAGAGTGATGATGAGTGGAGAAGAAGTGAGGTACTCAGCTGGGTTATTCTCAATCCCAAAGGCAGGCTACATAGTGAAAGCACCAGAAGAGCTTGTAGATGAACAACATCCTTTGCTCTTCAATCCATTTGATCATGTTCAATTTCTTCAGTTCTACTATACAGAAGCTGGCCAAAAAGCTCAATCTGCCCTCAAAACTTATTGTGGTGCAACTTAG
Homology
[Close]
BLAST of CSPI04G00590 vs. Swiss-Prot
Match: AOP1L_ARATH (Probable 2-oxoglutarate-dependent dioxygenase AOP1.2 OS=Arabidopsis thaliana GN=AOP1.2 PE=2 SV=1)

HSP 1 Score: 364.0 bits (933), Expect = 1.7e-99
Identity = 176/312 (56.41%), Postives = 231/312 (74.04%), Query Frame = 1 

Query: 6   SLKLPLIDFS--NLESGGPKWELAKAQVKEALEEFGCFEASFDKVPVEVRKGLFEALEEL 65
           S +LP+IDFS  NL+ G  KW+  KA V +ALE++GCFEA FDK+ VE+ + +FEA+E+L
Sbjct: 11  SFQLPVIDFSDQNLKPGSSKWDEVKADVLKALEDYGCFEAFFDKLSVELNRSVFEAMEDL 70

Query: 66  FNLPLETKLRNVSQKPFHGYVGQYPMAPLFESMGVDDSTIPQKVQDFTNILWPQ-GNPTF 125
           F LP+ TK RNVS KPFHGY+       L+ES+G+DD+ + +KV DFT  LWP  GN + 
Sbjct: 71  FELPIPTKQRNVSSKPFHGYLCHN----LYESLGIDDANVLEKVNDFTQQLWPDHGNKSI 130

Query: 126 SKVMETYSQQLAELDEMVRRMVLESLGVEKYLEEHLESTNYLLRVMKYKGT----ESNYE 185
           S+ +  +S+QL ELD MVRRM++ES G+E Y++EHL ST YL R+MKY       + + E
Sbjct: 131 SETIHLFSEQLVELDLMVRRMIMESFGIENYIDEHLNSTYYLTRLMKYTSPPDDDDDDEE 190

Query: 186 TKIGLHSHTDKNIVTILYQNHVQGLQVKTKDGKWINFQPSPDSFVAMIGDSFHAWTNGRL 245
           TK+GL SHTDKNI+TIL+Q  V GL+VKTKD KWI  +PS DS + M+GDS  A  NGRL
Sbjct: 191 TKLGLRSHTDKNIITILHQYQVDGLEVKTKDDKWIKVKPSQDSVLVMVGDSLCALLNGRL 250

Query: 246 HSPYHRVMMSGEEVRYSAGLFSIPKAGYIVKAPEELVDEQHPLLFNPFDHVQFLQFYYTE 305
           HSPYHRV+M+G++ RYS GLFSIPK G I+ +PEELVD++HP +F PF++  FL F+ TE
Sbjct: 251 HSPYHRVIMTGKKTRYSTGLFSIPKTGVIIDSPEELVDKEHPRIFKPFEYTDFLHFFQTE 310

Query: 306 AGQKAQSALKTY 311
           AG+ AQSAL  +
Sbjct: 311 AGRIAQSALHAF 318

[Close]
BLAST of CSPI04G00590 vs. Swiss-Prot
Match: AOP1C_ARATH (Probable 2-oxoglutarate-dependent dioxygenase AOP1 OS=Arabidopsis thaliana GN=AOP1 PE=2 SV=1)

HSP 1 Score: 361.7 bits (927), Expect = 8.2e-99
Identity = 175/313 (55.91%), Postives = 231/313 (73.80%), Query Frame = 1 

Query: 6   SLKLPLIDFS--NLESGGPKWELAKAQVKEALEEFGCFEASFDKVPVEVRKGLFEALEEL 65
           S +LP+IDFS  NL+ G  KW+   A V +ALE++GCFEASFDK+ VE+ + +FEA+E+L
Sbjct: 11  SFQLPVIDFSDQNLKPGSSKWDEVTADVLKALEDYGCFEASFDKLSVELNRSVFEAMEDL 70

Query: 66  FNLPLETKLRNVSQKPFHGYVGQYPMAPLFESMGVDDSTIPQKVQDFTNILWPQ-GNPTF 125
           F LP+ TK RNVS KPFHGY+       L+ES+G++D+ + +KV DFT  LWP  GN + 
Sbjct: 71  FELPIPTKQRNVSSKPFHGYLCHN----LYESLGINDANVLEKVNDFTQQLWPDHGNKSI 130

Query: 126 SKVMETYSQQLAELDEMVRRMVLESLGVEKYLEEHLESTNYLLRVMKYKGT-----ESNY 185
           S+ +  +S+QL ELD MVRRM++ES G+E Y++EHL ST YL R+MKY        + + 
Sbjct: 131 SETIHLFSEQLVELDLMVRRMIMESFGIENYIDEHLNSTYYLTRLMKYTSPPDDDDDDDE 190

Query: 186 ETKIGLHSHTDKNIVTILYQNHVQGLQVKTKDGKWINFQPSPDSFVAMIGDSFHAWTNGR 245
           ETK+GL SHTDKNI+TIL+Q  V GL+VKTKD KWI  +PS DS + M+GDS  A  NGR
Sbjct: 191 ETKLGLRSHTDKNIITILHQYQVDGLEVKTKDDKWIKVKPSQDSVLVMVGDSLCALLNGR 250

Query: 246 LHSPYHRVMMSGEEVRYSAGLFSIPKAGYIVKAPEELVDEQHPLLFNPFDHVQFLQFYYT 305
           LHSPYHRV+M+G++ RYS GLFSIPK G I+ +PEELVD++HP +F PF++  FL F+ T
Sbjct: 251 LHSPYHRVIMTGKKTRYSTGLFSIPKTGVIIDSPEELVDKEHPRIFKPFEYTDFLHFFQT 310

Query: 306 EAGQKAQSALKTY 311
           EAG+ AQSAL  +
Sbjct: 311 EAGRIAQSALHAF 319

[Close]
BLAST of CSPI04G00590 vs. Swiss-Prot
Match: AOP1V_ARATH (Probable 2-oxoglutarate-dependent dioxygenase AOP1 (Fragment) OS=Arabidopsis thaliana GN=AOP1 PE=2 SV=1)

HSP 1 Score: 352.4 bits (903), Expect = 5.0e-96
Identity = 170/302 (56.29%), Postives = 225/302 (74.50%), Query Frame = 1 

Query: 6   SLKLPLIDFS--NLESGGPKWELAKAQVKEALEEFGCFEASFDKVPVEVRKGLFEALEEL 65
           S +LP+IDFS  NL+ G  KW+   A V +ALE++GCFEASFDK+ VE+ + +FEA+E+L
Sbjct: 4   SFQLPVIDFSDQNLKPGSSKWDEVTADVLKALEDYGCFEASFDKLSVELNRSVFEAMEDL 63

Query: 66  FNLPLETKLRNVSQKPFHGYVGQYPMAPLFESMGVDDSTIPQKVQDFTNILWPQ-GNPTF 125
           F LP+ TK RNVS K FHGY+       L+ES+G++D+ + +KV DFT  LWP  GN + 
Sbjct: 64  FELPIPTKQRNVSSKLFHGYLCHN----LYESLGINDANVLEKVNDFTQQLWPDHGNKSI 123

Query: 126 SKVMETYSQQLAELDEMVRRMVLESLGVEKYLEEHLESTNYLLRVMKYKGT---ESNYET 185
           S+ +  +S+QL ELD MVRRM++ES G+EKY++EHL ST YL R+MKY      + + ET
Sbjct: 124 SETIHLFSEQLVELDLMVRRMIMESFGIEKYIDEHLNSTYYLTRLMKYTSPPDDDDDEET 183

Query: 186 KIGLHSHTDKNIVTILYQNHVQGLQVKTKDGKWINFQPSPDSFVAMIGDSFHAWTNGRLH 245
           K+GL SHTDKNI+TIL+Q  V GL+VKTKD KWI  +PS DS + M+GDS  A  NGRLH
Sbjct: 184 KLGLRSHTDKNIITILHQYQVDGLEVKTKDDKWIKVKPSQDSVLVMVGDSLCALLNGRLH 243

Query: 246 SPYHRVMMSGEEVRYSAGLFSIPKAGYIVKAPEELVDEQHPLLFNPFDHVQFLQFYYTEA 302
           SPYHRV+M+G++ RYS GLFSIPK G I+ +PEELVD++HP +F PF++  FL F+ TEA
Sbjct: 244 SPYHRVIMTGKKTRYSTGLFSIPKTGVIIDSPEELVDKEHPRIFKPFEYTDFLHFFQTEA 301

[Close]
BLAST of CSPI04G00590 vs. Swiss-Prot
Match: AOP2V_ARATH (2-oxoglutarate-dependent dioxygenase AOP2 OS=Arabidopsis thaliana GN=AOP2 PE=2 SV=1)

HSP 1 Score: 166.8 bits (421), Expect = 3.9e-40
Identity = 80/169 (47.34%), Postives = 123/169 (72.78%), Query Frame = 1 

Query: 6   SLKLPLIDFSN--LESGGPKWELAKAQVKEALEEFGCFEASFDKVPVEVRKGLFEALEEL 65
           SL+LPLI+ ++  LE G  KW   ++ V++ALE+FGCFEAS+DKV +E+++ + + +EEL
Sbjct: 5   SLQLPLINLADKTLEPGSSKWAEVRSDVRKALEDFGCFEASYDKVSLELQESIMKTMEEL 64

Query: 66  FNLPLETKLRNVSQKPFHGYVGQYPMAPLFESMGVDDSTIPQKVQDFTNILWP--QGNPT 125
           F LP+ETK RNV  KP+ GY+    ++   ES+G+ ++ I + V +FT  LWP   GN  
Sbjct: 65  FALPVETKQRNVCPKPYVGYLNHNNLS---ESLGISNANILENVNEFTQQLWPDGDGNEN 124

Query: 126 FSKVMETYSQQLAELDEMVRRMVLESLGVEKYLEEHLESTNYLLRVMKY 171
            SK ++ ++++L E+D MVRRMV+ES G+EKY+++HL+ST Y +R+MKY
Sbjct: 125 ISKTIQLFAEKLVEIDVMVRRMVMESFGIEKYIDDHLKSTEYRMRLMKY 170

[Close]
BLAST of CSPI04G00590 vs. Swiss-Prot
Match: AOP2C_ARATH (Probable inactive 2-oxoglutarate-dependent dioxygenase AOP2 OS=Arabidopsis thaliana GN=AOP2 PE=2 SV=1)

HSP 1 Score: 165.2 bits (417), Expect = 1.1e-39
Identity = 70/145 (48.28%), Postives = 105/145 (72.41%), Query Frame = 1 

Query: 171 KGTESNYETKIGLHSHTDKNIVTILYQNHVQGLQVKTKDGKWINFQPSPDSFVAMIGDSF 230
           +  + N E K+GL  HTDKN+ T+L+Q+ ++GL+VKTKD KWI  +PSP++F+ + GDS 
Sbjct: 253 EANDDNEEKKLGLPCHTDKNLFTVLFQHEIEGLEVKTKDEKWIRVKPSPNTFIVIAGDSL 312

Query: 231 HAWTNGRLHSPYHRVMMSGEE-VRYSAGLFSIPKAGYIVKAPEELVDEQHPLLFNPFDHV 290
            A  NGR+ +PYHRV ++ ++  RY+A +F+ PK  Y+++AP+ELVDE+HP LF PFD+ 
Sbjct: 313 CALMNGRIRAPYHRVRVTEKKRTRYTAAIFTCPKPDYVIEAPKELVDEKHPRLFRPFDYR 372

Query: 291 QFLQFYYTEAGQKAQSALKTYCGAT 315
               FY++EAG+K Q  L+ YC  +
Sbjct: 373 DLFTFYHSEAGRKIQYTLQAYCAVS 397

[Close]
BLAST of CSPI04G00590 vs. TrEMBL
Match: M5WXX0_PRUPE (Uncharacterized protein OS=Prunus persica GN=PRUPE_ppa021358mg PE=3 SV=1)

HSP 1 Score: 492.7 bits (1267), Expect = 3.4e-136
Identity = 228/314 (72.61%), Postives = 275/314 (87.58%), Query Frame = 1 

Query: 1   MDSESSLKLPLIDFSN--LESGGPKWELAKAQVKEALEEFGCFEASFDKVPVEVRKGLFE 60
           M SE+ LKLP+IDFSN  L  G  +W   +AQV  ALEE+GCFEA F+KVP  +RK +F 
Sbjct: 1   MGSETPLKLPVIDFSNQSLTPGTTEWNTVRAQVHNALEEYGCFEALFNKVPPHIRKSIFP 60

Query: 61  ALEELFNLPLETKLRNVSQKPFHGYVGQYPMAPLFESMGVDDSTIPQKVQDFTNILWPQG 120
           ++EELF+LPL+TKL+NVS+KPFHGYVGQYPM PL+ESMGVDD+ + ++VQ  TN LWPQG
Sbjct: 61  SIEELFDLPLQTKLQNVSKKPFHGYVGQYPMVPLYESMGVDDANVYEQVQSLTNTLWPQG 120

Query: 121 NPTFSKVMETYSQQLAELDEMVRRMVLESLGVEKYLEEHLESTNYLLRVMKYKGTESNYE 180
           NP+F K ++++S+Q++ELD+++RRM+LESLG+EKYLEEHLESTNYLLRVMKYKG ++N E
Sbjct: 121 NPSFCKTIQSFSEQVSELDQIIRRMILESLGLEKYLEEHLESTNYLLRVMKYKGPQTN-E 180

Query: 181 TKIGLHSHTDKNIVTILYQNHVQGLQVKTKDGKWINFQPSPDSFVAMIGDSFHAWTNGRL 240
           TK+GL++HTDKNIVTILYQN V GL+V+ KDGKWIN +PSPDSF+AMIGDS +AWTNGRL
Sbjct: 181 TKLGLNAHTDKNIVTILYQNQVDGLEVQNKDGKWINVKPSPDSFIAMIGDSLYAWTNGRL 240

Query: 241 HSPYHRVMMSGEEVRYSAGLFSIPKAGYIVKAPEELVDEQHPLLFNPFDHVQFLQFYYTE 300
           HSPYHRVMMSG EVRYS GLFSIPK GYI+KAPEE+VDE+HPLLF PFDHVQFL FYY+E
Sbjct: 241 HSPYHRVMMSGNEVRYSTGLFSIPKEGYIIKAPEEVVDEEHPLLFKPFDHVQFLGFYYSE 300

Query: 301 AGQKAQSALKTYCG 313
           AGQ+A SALKTYCG
Sbjct: 301 AGQRAHSALKTYCG 313

[Close]
BLAST of CSPI04G00590 vs. TrEMBL
Match: D7U0Q6_VITVI (Putative uncharacterized protein OS=Vitis vinifera GN=VIT_09s0002g05280 PE=3 SV=1)

HSP 1 Score: 481.9 bits (1239), Expect = 6.1e-133
Identity = 222/314 (70.70%), Postives = 275/314 (87.58%), Query Frame = 1 

Query: 1   MDSESSLKLPLIDFSN--LESGGPKWELAKAQVKEALEEFGCFEASFDKVPVEVRKGLFE 60
           M SE+ LKLP+IDFS+  L+ G  +W L KAQV++ALEE+GCFEA F+KVP+++RK +F 
Sbjct: 1   MGSETPLKLPVIDFSDPELKPGTLQWNLVKAQVQKALEEYGCFEAFFNKVPLDLRKAIFG 60

Query: 61  ALEELFNLPLETKLRNVSQKPFHGYVGQYPMAPLFESMGVDDSTIPQKVQDFTNILWPQG 120
           ALEELF+LPL+TKLRN S+KP+HGYVGQYP  PL+ESMG+DD+ + +K + FT +LWP+G
Sbjct: 61  ALEELFDLPLQTKLRNSSKKPYHGYVGQYPTVPLYESMGIDDALVQEKAEHFTKLLWPEG 120

Query: 121 NPTFSKVMETYSQQLAELDEMVRRMVLESLGVEKYLEEHLESTNYLLRVMKYKGTESNYE 180
           NP F K ++++S+QL+ELD  +RRMVLESLG+EKYL+EH++STNYLLRVMKYKG ++  E
Sbjct: 121 NPNFCKTIQSFSEQLSELDHTIRRMVLESLGMEKYLDEHMDSTNYLLRVMKYKGPQTT-E 180

Query: 181 TKIGLHSHTDKNIVTILYQNHVQGLQVKTKDGKWINFQPSPDSFVAMIGDSFHAWTNGRL 240
           TK+GL++HTDKNIVTILYQN V GL V+TKDG+WIN + SPDSF+ MIGDSF AWTNGRL
Sbjct: 181 TKLGLNAHTDKNIVTILYQNQVDGLGVQTKDGQWINIKCSPDSFIIMIGDSFLAWTNGRL 240

Query: 241 HSPYHRVMMSGEEVRYSAGLFSIPKAGYIVKAPEELVDEQHPLLFNPFDHVQFLQFYYTE 300
           HSPYHRVMM+G E RYS GLFSIPKAGY++KAPEELVDE+HPLLF PFDH++FLQFYYTE
Sbjct: 241 HSPYHRVMMTGNESRYSTGLFSIPKAGYVIKAPEELVDEEHPLLFKPFDHLEFLQFYYTE 300

Query: 301 AGQKAQSALKTYCG 313
           AGQ+A+SALKTYCG
Sbjct: 301 AGQRAESALKTYCG 313

[Close]
BLAST of CSPI04G00590 vs. TrEMBL
Match: K7KZF3_SOYBN (Uncharacterized protein OS=Glycine max GN=GLYMA_07G033700 PE=3 SV=1)

HSP 1 Score: 480.7 bits (1236), Expect = 1.3e-132
Identity = 217/314 (69.11%), Postives = 276/314 (87.90%), Query Frame = 1 

Query: 1   MDSESSLKLPLIDFSNL--ESGGPKWELAKAQVKEALEEFGCFEASFDKVPVEVRKGLFE 60
           M SE++LKLP+IDF+NL  E+  P WE  K+QV +AL ++GCFEA F+KVP+E+RK +F 
Sbjct: 1   MGSEATLKLPVIDFTNLKLEANNPNWEAIKSQVHKALVDYGCFEAIFEKVPLELRKAIFA 60

Query: 61  ALEELFNLPLETKLRNVSQKPFHGYVGQYPMAPLFESMGVDDSTIPQKVQDFTNILWPQG 120
           AL+ELF+LPL+TK+ NVS+KP+HGYVGQYPM PLFESMG+DD+ + + V+  TNI+WP G
Sbjct: 61  ALQELFDLPLQTKILNVSKKPYHGYVGQYPMVPLFESMGIDDANVYENVESMTNIMWPHG 120

Query: 121 NPTFSKVMETYSQQLAELDEMVRRMVLESLGVEKYLEEHLESTNYLLRVMKYKGTESNYE 180
           NP+FSK ++++S+QL+ELD+++R+M+LESLGVEKYLEEH+ STNYLLRVMKYKG +++ +
Sbjct: 121 NPSFSKTIQSFSEQLSELDQIIRKMILESLGVEKYLEEHMNSTNYLLRVMKYKGPQTS-D 180

Query: 181 TKIGLHSHTDKNIVTILYQNHVQGLQVKTKDGKWINFQPSPDSFVAMIGDSFHAWTNGRL 240
           TK+GL +H+DKNIVTILYQN V+GL+V TKDGKWI+++PSPDSFV MIGDS HAW+NGRL
Sbjct: 181 TKVGLTTHSDKNIVTILYQNEVEGLEVMTKDGKWISYRPSPDSFVVMIGDSLHAWSNGRL 240

Query: 241 HSPYHRVMMSGEEVRYSAGLFSIPKAGYIVKAPEELVDEQHPLLFNPFDHVQFLQFYYTE 300
           HSP+HRVMMSG E RYSAGLFSIPK G I+KAPEELVDE+HPLLF PFDHV+FL++YYTE
Sbjct: 241 HSPFHRVMMSGNEARYSAGLFSIPKGGNIIKAPEELVDEEHPLLFKPFDHVEFLKYYYTE 300

Query: 301 AGQKAQSALKTYCG 313
            GQ+ Q AL+TYCG
Sbjct: 301 KGQRDQFALRTYCG 313

[Close]
BLAST of CSPI04G00590 vs. TrEMBL
Match: A5AJH9_VITVI (Putative uncharacterized protein OS=Vitis vinifera GN=VITISV_028512 PE=3 SV=1)

HSP 1 Score: 480.3 bits (1235), Expect = 1.8e-132
Identity = 222/314 (70.70%), Postives = 273/314 (86.94%), Query Frame = 1 

Query: 1   MDSESSLKLPLIDFSN--LESGGPKWELAKAQVKEALEEFGCFEASFDKVPVEVRKGLFE 60
           M SE+ LKLP IDFS+  L+ G  +W L KAQV++ALEE+GCFEA F+KVP+++RK +F 
Sbjct: 1   MGSETPLKLPXIDFSDPELKPGTLQWNLVKAQVQKALEEYGCFEAFFNKVPLDLRKAIFG 60

Query: 61  ALEELFNLPLETKLRNVSQKPFHGYVGQYPMAPLFESMGVDDSTIPQKVQDFTNILWPQG 120
           ALEELF+LPL+TKLRN S+KP+HGYVGQYP  PL+ESMG+DD+ + +K + FT +LWP+G
Sbjct: 61  ALEELFDLPLQTKLRNSSKKPYHGYVGQYPTVPLYESMGIDDALVQEKAEHFTKLLWPEG 120

Query: 121 NPTFSKVMETYSQQLAELDEMVRRMVLESLGVEKYLEEHLESTNYLLRVMKYKGTESNYE 180
           NP F K ++++S+QL+ELD  +RRMVLESLG+EKYL+EH++STNYLLRVMKYKG ++  E
Sbjct: 121 NPNFCKTIQSFSEQLSELDHTIRRMVLESLGMEKYLDEHMDSTNYLLRVMKYKGPQTT-E 180

Query: 181 TKIGLHSHTDKNIVTILYQNHVQGLQVKTKDGKWINFQPSPDSFVAMIGDSFHAWTNGRL 240
           TK+GL++HTDKNIVTILYQN V GL V+TKDG WIN + SPDSF+ MIGDSF AWTNGRL
Sbjct: 181 TKLGLNAHTDKNIVTILYQNQVDGLGVQTKDGXWINIKCSPDSFIIMIGDSFLAWTNGRL 240

Query: 241 HSPYHRVMMSGEEVRYSAGLFSIPKAGYIVKAPEELVDEQHPLLFNPFDHVQFLQFYYTE 300
           HSPYHRVMM+G E RYS GLFSIPKAGY++KAPEELVDE+HPLLF PFDH++FLQFYYTE
Sbjct: 241 HSPYHRVMMTGNESRYSTGLFSIPKAGYVIKAPEELVDEEHPLLFKPFDHLEFLQFYYTE 300

Query: 301 AGQKAQSALKTYCG 313
           AGQ+A+SALKTYCG
Sbjct: 301 AGQRAESALKTYCG 313

[Close]
BLAST of CSPI04G00590 vs. TrEMBL
Match: A0A0B2NW45_GLYSO (Gibberellin 20 oxidase 1 OS=Glycine soja GN=glysoja_009567 PE=3 SV=1)

HSP 1 Score: 478.8 bits (1231), Expect = 5.1e-132
Identity = 216/314 (68.79%), Postives = 275/314 (87.58%), Query Frame = 1 

Query: 1   MDSESSLKLPLIDFSNL--ESGGPKWELAKAQVKEALEEFGCFEASFDKVPVEVRKGLFE 60
           M SE++LKLP+IDF+NL  E+  P WE  K+QV +AL ++GCFEA F+KVP+E+RK +F 
Sbjct: 1   MGSEATLKLPVIDFTNLKLEANNPNWEAIKSQVHKALVDYGCFEAIFEKVPLELRKAIFA 60

Query: 61  ALEELFNLPLETKLRNVSQKPFHGYVGQYPMAPLFESMGVDDSTIPQKVQDFTNILWPQG 120
           AL+ELF+LPL+TK+ NVS+KP+HGYVGQYPM PLFESMG+DD+ + + V+  TNI+WP G
Sbjct: 61  ALQELFDLPLQTKILNVSKKPYHGYVGQYPMVPLFESMGIDDANVYENVESMTNIMWPHG 120

Query: 121 NPTFSKVMETYSQQLAELDEMVRRMVLESLGVEKYLEEHLESTNYLLRVMKYKGTESNYE 180
           NP+FSK ++++S+QL+ELD+++R+M+LESLGVEKYLEEH+ STNYLL VMKYKG +++ +
Sbjct: 121 NPSFSKTIQSFSEQLSELDQIIRKMILESLGVEKYLEEHMNSTNYLLEVMKYKGPQTS-D 180

Query: 181 TKIGLHSHTDKNIVTILYQNHVQGLQVKTKDGKWINFQPSPDSFVAMIGDSFHAWTNGRL 240
           TK+GL +H+DKNIVTILYQN V+GL+V TKDGKWI+++PSPDSFV MIGDS HAW+NGRL
Sbjct: 181 TKVGLTTHSDKNIVTILYQNEVEGLEVMTKDGKWISYRPSPDSFVVMIGDSLHAWSNGRL 240

Query: 241 HSPYHRVMMSGEEVRYSAGLFSIPKAGYIVKAPEELVDEQHPLLFNPFDHVQFLQFYYTE 300
           HSP+HRVMMSG E RYSAGLFSIPK G I+KAPEELVDE+HPLLF PFDHV+FL++YYTE
Sbjct: 241 HSPFHRVMMSGNEARYSAGLFSIPKGGNIIKAPEELVDEEHPLLFKPFDHVEFLKYYYTE 300

Query: 301 AGQKAQSALKTYCG 313
            GQ+ Q AL+TYCG
Sbjct: 301 KGQRDQFALRTYCG 313

[Close]
BLAST of CSPI04G00590 vs. TAIR10
Match: AT1G52820.1 (AT1G52820.1 2-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily protein)

HSP 1 Score: 455.3 bits (1170), Expect = 3.1e-128
Identity = 210/308 (68.18%), Postives = 265/308 (86.04%), Query Frame = 1 

Query: 7   LKLPLIDFSN--LESGGPKWELAKAQVKEALEEFGCFEASFDKVPVEVRKGLFEALEELF 66
           L+LP+IDFSN  L+ G P+W+L +A V++AL+++G FEASFD++P E+RK +F ALEELF
Sbjct: 10  LRLPVIDFSNKNLKPGEPEWDLTRADVQKALQDYGYFEASFDRIPFELRKSVFGALEELF 69

Query: 67  NLPLETKLRNVSQKPFHGYVGQYPMAPLFESMGVDDSTIPQKVQDFTNILWPQGNPTFSK 126
           +LPL+TKLRNVS+KPFHGYVGQYPM PL+ESMG+DDS I +KV  FT  LWPQGN +FS 
Sbjct: 70  DLPLQTKLRNVSKKPFHGYVGQYPMVPLYESMGIDDSDIAEKVDAFTEKLWPQGNISFST 129

Query: 127 VMETYSQQLAELDEMVRRMVLESLGVEKYLEEHLESTNYLLRVMKYKGTESNYETKIGLH 186
            ++++S++L+ELD  +RRM++ES G++KY++EHL STNYLLRVMKYKG ++  ETK+GL+
Sbjct: 130 TIQSFSKKLSELDITIRRMIMESFGLDKYIDEHLHSTNYLLRVMKYKGPDTE-ETKVGLN 189

Query: 187 SHTDKNIVTILYQNHVQGLQVKTKDGKWINFQPSPDSFVAMIGDSFHAWTNGRLHSPYHR 246
           +HTDKNIVTILYQNHV+GL+V+TKD  WI  +P+ DSF  MIGDS +A  NGRLHSPYHR
Sbjct: 190 AHTDKNIVTILYQNHVEGLEVQTKDKNWIKVKPTQDSFTVMIGDSLYALLNGRLHSPYHR 249

Query: 247 VMMSGEEVRYSAGLFSIPKAGYIVKAPEELVDEQHPLLFNPFDHVQFLQFYYTEAGQKAQ 306
           VMM+G E RYS GLFSIPKAG+IV +P+ELVDE+HP LF PFDHV+FLQFYYTEAGQ++Q
Sbjct: 250 VMMTGTETRYSLGLFSIPKAGHIVSSPDELVDEEHPRLFKPFDHVEFLQFYYTEAGQRSQ 309

Query: 307 SALKTYCG 313
           SALKTYCG
Sbjct: 310 SALKTYCG 316

[Close]
BLAST of CSPI04G00590 vs. TAIR10
Match: AT4G03070.1 (AT4G03070.1 2-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily protein)

HSP 1 Score: 361.7 bits (927), Expect = 4.6e-100
Identity = 175/313 (55.91%), Postives = 231/313 (73.80%), Query Frame = 1 

Query: 6   SLKLPLIDFS--NLESGGPKWELAKAQVKEALEEFGCFEASFDKVPVEVRKGLFEALEEL 65
           S +LP+IDFS  NL+ G  KW+   A V +ALE++GCFEASFDK+ VE+ + +FEA+E+L
Sbjct: 11  SFQLPVIDFSDQNLKPGSSKWDEVTADVLKALEDYGCFEASFDKLSVELNRSVFEAMEDL 70

Query: 66  FNLPLETKLRNVSQKPFHGYVGQYPMAPLFESMGVDDSTIPQKVQDFTNILWPQ-GNPTF 125
           F LP+ TK RNVS KPFHGY+       L+ES+G++D+ + +KV DFT  LWP  GN + 
Sbjct: 71  FELPIPTKQRNVSSKPFHGYLCHN----LYESLGINDANVLEKVNDFTQQLWPDHGNKSI 130

Query: 126 SKVMETYSQQLAELDEMVRRMVLESLGVEKYLEEHLESTNYLLRVMKYKGT-----ESNY 185
           S+ +  +S+QL ELD MVRRM++ES G+E Y++EHL ST YL R+MKY        + + 
Sbjct: 131 SETIHLFSEQLVELDLMVRRMIMESFGIENYIDEHLNSTYYLTRLMKYTSPPDDDDDDDE 190

Query: 186 ETKIGLHSHTDKNIVTILYQNHVQGLQVKTKDGKWINFQPSPDSFVAMIGDSFHAWTNGR 245
           ETK+GL SHTDKNI+TIL+Q  V GL+VKTKD KWI  +PS DS + M+GDS  A  NGR
Sbjct: 191 ETKLGLRSHTDKNIITILHQYQVDGLEVKTKDDKWIKVKPSQDSVLVMVGDSLCALLNGR 250

Query: 246 LHSPYHRVMMSGEEVRYSAGLFSIPKAGYIVKAPEELVDEQHPLLFNPFDHVQFLQFYYT 305
           LHSPYHRV+M+G++ RYS GLFSIPK G I+ +PEELVD++HP +F PF++  FL F+ T
Sbjct: 251 LHSPYHRVIMTGKKTRYSTGLFSIPKTGVIIDSPEELVDKEHPRIFKPFEYTDFLHFFQT 310

Query: 306 EAGQKAQSALKTY 311
           EAG+ AQSAL  +
Sbjct: 311 EAGRIAQSALHAF 319

[Close]
BLAST of CSPI04G00590 vs. TAIR10
Match: AT1G52800.1 (AT1G52800.1 2-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily protein)

HSP 1 Score: 290.8 bits (743), Expect = 1.0e-78
Identity = 140/308 (45.45%), Postives = 198/308 (64.29%), Query Frame = 1 

Query: 8   KLPLIDFSNLESGGPK---WELAKAQVKEALEEFGCFEASFDKVPVEVRKGLFEALEELF 67
           K+P++D ++ +   P    W     +  EALEE+GCF A +D V  ++   +F A EELF
Sbjct: 9   KVPILDLTSQQELKPNTSTWRSVSREACEALEEYGCFLAVYDGVTQQLDDSIFAAAEELF 68

Query: 68  NLPLETKLRNVSQKPFHGYVGQYPMAPLFESMGVDDSTIPQKVQDFTNILWPQGNPTFSK 127
           +LP ETK +NV++KP+HGYVGQ P+ PL E +GVD  T  +  Q FT+++WPQGN  F  
Sbjct: 69  DLPTETKKKNVNEKPYHGYVGQMPVIPLHEGLGVDYVTNKEIAQRFTHLMWPQGNDRFCN 128

Query: 128 VMETYSQQLAELDEMVRRMVLESLGVEKYLEEHLESTNYLLRVMKYKGTESNYETKIGLH 187
            + T+S  +AELD +V RM+ E+ GVEK+ E H+ S  YLL+ +KY     +        
Sbjct: 129 TVHTFSNAVAELDRLVVRMIFENYGVEKHYESHVGSKTYLLKFLKYLAPPESISMP-AFP 188

Query: 188 SHTDKNIVTILYQNHVQGLQVKTKDGKWINFQPSPDSFVAMIGDSFHAWTNGRLHSPYHR 247
            HTDK  ++IL+QN V GL+VK+KDG+WI+ Q  P S+V M GD    W+N R+ S  HR
Sbjct: 189 QHTDKTFLSILHQNDVNGLEVKSKDGEWISLQLPPKSYVVMAGDISMGWSNDRIRSCEHR 248

Query: 248 VMMSGEEVRYSAGLFSIPKAGYIVKAPEELVDEQHPLLFNPFDHVQFLQFYYTEAGQKAQ 307
           V M G++ RY+ GLFS      +V  PEELVD++HPL++ PFD++  + FY T+ G++A 
Sbjct: 249 VTMEGDKTRYTLGLFSF--LTDLVSIPEELVDDKHPLMYKPFDNIALINFYTTKEGREAN 308

Query: 308 SALKTYCG 313
           S LK YCG
Sbjct: 309 STLKAYCG 313

[Close]
BLAST of CSPI04G00590 vs. TAIR10
Match: AT1G52790.1 (AT1G52790.1 2-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily protein)

HSP 1 Score: 288.1 bits (736), Expect = 6.5e-78
Identity = 138/311 (44.37%), Postives = 210/311 (67.52%), Query Frame = 1 

Query: 6   SLKLPLIDFS--NLESGGPKWELAKAQVKEALEEFGCFEASF-DKVPVEVRKGLFEALEE 65
           S K+P +DFS  +L+ G   WE  +  +++ALEE+GCF     DK P+++   +F +L +
Sbjct: 2   SPKIPTLDFSREDLKPGTKYWESTRENIRQALEEYGCFIIDLKDKTPLDLLDRVFGSLVD 61

Query: 66  LFNLPLETKLRNVSQKPFHGYVGQYPMAPLFESMGVDDSTIPQKVQDFTNILWPQGNPTF 125
           LF+LP +TK++N   KP +GYVGQ P  PL ES+G+D++T  +  + FT ++WPQGN  F
Sbjct: 62  LFDLPTQTKMKNKYDKPLNGYVGQIPALPLHESLGIDNATSLEATRSFTGLMWPQGNEHF 121

Query: 126 SKVMETYSQQLAELDEMVRRMVLESLGVEKYLEEHLESTNYLLRVMKYKGTESNYETKIG 185
           S+ +  Y++  AELD+MV RMV +S  VEKY + ++EST YLLRV+K +   +N    +G
Sbjct: 122 SECLYKYAEFAAELDQMVTRMVFQSYNVEKYYDPYIESTTYLLRVLKNR-APNNENPTLG 181

Query: 186 LHSHTDKNIVTILYQNHVQGLQVKTKDGKWINFQ-PSPDSFVAMIGDSFHAWTNGRLHSP 245
             +HTDK+  TIL+Q+ V GL+++T++G+ IN    SP  F+ + GD+  AW+N R+ SP
Sbjct: 182 FVTHTDKSFTTILHQDQVNGLEMETREGERININLSSPSLFMVVAGDALMAWSNDRVWSP 241

Query: 246 YHRVMMSGEEVRYSAGLFSIPKAGYIVKAPEELVDEQHPLLFNPFDHVQFLQFYYTEAGQ 305
            H+V++SGE  RYS G+F+       ++ PEEL+D QHPL++ PFDH+  L+FY T+ G 
Sbjct: 242 RHQVLVSGETDRYSLGMFAFNNG--TLQVPEELIDHQHPLMYKPFDHLGLLRFYRTDTGY 301

Query: 306 KAQSALKTYCG 313
           K++  +K YCG
Sbjct: 302 KSECPIKAYCG 309

[Close]
BLAST of CSPI04G00590 vs. TAIR10
Match: AT1G28030.1 (AT1G28030.1 2-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily protein)

HSP 1 Score: 283.1 bits (723), Expect = 2.1e-76
Identity = 148/316 (46.84%), Postives = 209/316 (66.14%), Query Frame = 1 

Query: 2   DSESSLKLPLIDFSN--LESGGPKWELAKAQVKEALEEFGCFEASFDKVPVEVRKGLFEA 61
           +S+  L LP+IDFSN  L+   P+W+L ++QV++ALEE+GCFEA FD   +E+RK LFE+
Sbjct: 6   ESQVPLSLPIIDFSNPDLKPETPEWDLVRSQVRKALEEYGCFEALFDGASMELRKALFES 65

Query: 62  LEELFNLPLETKLRNVSQKPFHGYVGQYPMAPLFESMGVDDSTIPQKVQDFTNILWPQGN 121
            +E+F+LPLETKL   +   + GY+   P  P+ E MG      P  V D T+ LWPQGN
Sbjct: 66  SKEVFDLPLETKLSTKTDVHYEGYL-TIPRVPIQEGMGFYGIDNPNVVNDLTHKLWPQGN 125

Query: 122 PTFSKVMETYSQQLAELDEMVRRMVLESLGVEKYLEEHLESTNYLLRVMKYKG-TESNYE 181
               K +++++++L EL+  VR M LES G+EKY+EEHL + N   +++KYKG ++ N E
Sbjct: 126 IFVGKNVQSFAEKLIELNLTVRTMTLESFGLEKYMEEHLNAANKHFQLLKYKGISDDNTE 185

Query: 182 TKIGLHSHTDKNIVTILYQNH-VQGLQVKTKDGK-WINFQPSP-DSFVAMIGDSFHAWTN 241
            KIG + H D++ +TIL QN  V GL++KTKDG+ WI  +PS   SF+ M G S H   N
Sbjct: 186 NKIGFYPHIDRHFLTILCQNDAVDGLEIKTKDGEEWIKVKPSQASSFIVMAGASLHVLLN 245

Query: 242 GRLHSPYHRVMMSGEEVRYSAGLFSIPKAGYIVKAPEELVDEQHPLLFNPFDHVQFLQFY 301
           G +  P HRV+++G++ RY A LF+IPK G I+ APEE+VD++HP L+ PFD   FL+F 
Sbjct: 246 GGVFPPLHRVVITGKKDRYVAALFTIPKEGVIINAPEEMVDDEHPRLYKPFDFWGFLKFS 305

Query: 302 YTEAGQKAQSALKTYC 312
                +K  S L  YC
Sbjct: 306 NLPNARKDISDLTDYC 320

[Close]
BLAST of CSPI04G00590 vs. NCBI nr
Match: gi|449469106|ref|XP_004152262.1| (PREDICTED: probable 2-oxoglutarate-dependent dioxygenase AOP1 [Cucumis sativus])

HSP 1 Score: 645.6 bits (1664), Expect = 4.5e-182
Identity = 314/314 (100.00%), Postives = 314/314 (100.00%), Query Frame = 1 

Query: 1   MDSESSLKLPLIDFSNLESGGPKWELAKAQVKEALEEFGCFEASFDKVPVEVRKGLFEAL 60
           MDSESSLKLPLIDFSNLESGGPKWELAKAQVKEALEEFGCFEASFDKVPVEVRKGLFEAL
Sbjct: 1   MDSESSLKLPLIDFSNLESGGPKWELAKAQVKEALEEFGCFEASFDKVPVEVRKGLFEAL 60

Query: 61  EELFNLPLETKLRNVSQKPFHGYVGQYPMAPLFESMGVDDSTIPQKVQDFTNILWPQGNP 120
           EELFNLPLETKLRNVSQKPFHGYVGQYPMAPLFESMGVDDSTIPQKVQDFTNILWPQGNP
Sbjct: 61  EELFNLPLETKLRNVSQKPFHGYVGQYPMAPLFESMGVDDSTIPQKVQDFTNILWPQGNP 120

Query: 121 TFSKVMETYSQQLAELDEMVRRMVLESLGVEKYLEEHLESTNYLLRVMKYKGTESNYETK 180
           TFSKVMETYSQQLAELDEMVRRMVLESLGVEKYLEEHLESTNYLLRVMKYKGTESNYETK
Sbjct: 121 TFSKVMETYSQQLAELDEMVRRMVLESLGVEKYLEEHLESTNYLLRVMKYKGTESNYETK 180

Query: 181 IGLHSHTDKNIVTILYQNHVQGLQVKTKDGKWINFQPSPDSFVAMIGDSFHAWTNGRLHS 240
           IGLHSHTDKNIVTILYQNHVQGLQVKTKDGKWINFQPSPDSFVAMIGDSFHAWTNGRLHS
Sbjct: 181 IGLHSHTDKNIVTILYQNHVQGLQVKTKDGKWINFQPSPDSFVAMIGDSFHAWTNGRLHS 240

Query: 241 PYHRVMMSGEEVRYSAGLFSIPKAGYIVKAPEELVDEQHPLLFNPFDHVQFLQFYYTEAG 300
           PYHRVMMSGEEVRYSAGLFSIPKAGYIVKAPEELVDEQHPLLFNPFDHVQFLQFYYTEAG
Sbjct: 241 PYHRVMMSGEEVRYSAGLFSIPKAGYIVKAPEELVDEQHPLLFNPFDHVQFLQFYYTEAG 300

Query: 301 QKAQSALKTYCGAT 315
           QKAQSALKTYCGAT
Sbjct: 301 QKAQSALKTYCGAT 314

[Close]
BLAST of CSPI04G00590 vs. NCBI nr
Match: gi|659108754|ref|XP_008454372.1| (PREDICTED: probable 2-oxoglutarate-dependent dioxygenase AOP1 [Cucumis melo])

HSP 1 Score: 625.2 bits (1611), Expect = 6.4e-176
Identity = 301/314 (95.86%), Postives = 310/314 (98.73%), Query Frame = 1 

Query: 1   MDSESSLKLPLIDFSNLESGGPKWELAKAQVKEALEEFGCFEASFDKVPVEVRKGLFEAL 60
           MD ESSLKLPLIDFSNLESGGPKWELAKAQVKEALEEFGCFEASFDKVP+EVRKGLFEAL
Sbjct: 1   MDGESSLKLPLIDFSNLESGGPKWELAKAQVKEALEEFGCFEASFDKVPIEVRKGLFEAL 60

Query: 61  EELFNLPLETKLRNVSQKPFHGYVGQYPMAPLFESMGVDDSTIPQKVQDFTNILWPQGNP 120
           EELFNLPLETKLRNVSQKPFHGYVGQYPMAPLFESMGVDDSTIPQKVQDFTNILWPQGNP
Sbjct: 61  EELFNLPLETKLRNVSQKPFHGYVGQYPMAPLFESMGVDDSTIPQKVQDFTNILWPQGNP 120

Query: 121 TFSKVMETYSQQLAELDEMVRRMVLESLGVEKYLEEHLESTNYLLRVMKYKGTESNYETK 180
           TFSKV+ETYSQQL+ELDEMVRRMVLESLGVEKY+EEHLESTNYLLRVMKYKGT+ + ETK
Sbjct: 121 TFSKVIETYSQQLSELDEMVRRMVLESLGVEKYMEEHLESTNYLLRVMKYKGTDQSNETK 180

Query: 181 IGLHSHTDKNIVTILYQNHVQGLQVKTKDGKWINFQPSPDSFVAMIGDSFHAWTNGRLHS 240
           IGLHSHTDKNIVTILYQNHV+GLQV+TKDGKWINFQPSP SFVAMIGDSFHAWTNGRLHS
Sbjct: 181 IGLHSHTDKNIVTILYQNHVEGLQVQTKDGKWINFQPSPHSFVAMIGDSFHAWTNGRLHS 240

Query: 241 PYHRVMMSGEEVRYSAGLFSIPKAGYIVKAPEELVDEQHPLLFNPFDHVQFLQFYYTEAG 300
           PYHRVMMSGEEVRYSAGLFSIPKAGYIVKAPEELVDEQHPLLFNPFDHVQFLQFYYTEAG
Sbjct: 241 PYHRVMMSGEEVRYSAGLFSIPKAGYIVKAPEELVDEQHPLLFNPFDHVQFLQFYYTEAG 300

Query: 301 QKAQSALKTYCGAT 315
           QKAQSALKT+CGAT
Sbjct: 301 QKAQSALKTFCGAT 314

[Close]
BLAST of CSPI04G00590 vs. NCBI nr
Match: gi|595960175|ref|XP_007216862.1| (hypothetical protein PRUPE_ppa021358mg [Prunus persica])

HSP 1 Score: 492.7 bits (1267), Expect = 4.9e-136
Identity = 228/314 (72.61%), Postives = 275/314 (87.58%), Query Frame = 1 

Query: 1   MDSESSLKLPLIDFSN--LESGGPKWELAKAQVKEALEEFGCFEASFDKVPVEVRKGLFE 60
           M SE+ LKLP+IDFSN  L  G  +W   +AQV  ALEE+GCFEA F+KVP  +RK +F 
Sbjct: 1   MGSETPLKLPVIDFSNQSLTPGTTEWNTVRAQVHNALEEYGCFEALFNKVPPHIRKSIFP 60

Query: 61  ALEELFNLPLETKLRNVSQKPFHGYVGQYPMAPLFESMGVDDSTIPQKVQDFTNILWPQG 120
           ++EELF+LPL+TKL+NVS+KPFHGYVGQYPM PL+ESMGVDD+ + ++VQ  TN LWPQG
Sbjct: 61  SIEELFDLPLQTKLQNVSKKPFHGYVGQYPMVPLYESMGVDDANVYEQVQSLTNTLWPQG 120

Query: 121 NPTFSKVMETYSQQLAELDEMVRRMVLESLGVEKYLEEHLESTNYLLRVMKYKGTESNYE 180
           NP+F K ++++S+Q++ELD+++RRM+LESLG+EKYLEEHLESTNYLLRVMKYKG ++N E
Sbjct: 121 NPSFCKTIQSFSEQVSELDQIIRRMILESLGLEKYLEEHLESTNYLLRVMKYKGPQTN-E 180

Query: 181 TKIGLHSHTDKNIVTILYQNHVQGLQVKTKDGKWINFQPSPDSFVAMIGDSFHAWTNGRL 240
           TK+GL++HTDKNIVTILYQN V GL+V+ KDGKWIN +PSPDSF+AMIGDS +AWTNGRL
Sbjct: 181 TKLGLNAHTDKNIVTILYQNQVDGLEVQNKDGKWINVKPSPDSFIAMIGDSLYAWTNGRL 240

Query: 241 HSPYHRVMMSGEEVRYSAGLFSIPKAGYIVKAPEELVDEQHPLLFNPFDHVQFLQFYYTE 300
           HSPYHRVMMSG EVRYS GLFSIPK GYI+KAPEE+VDE+HPLLF PFDHVQFL FYY+E
Sbjct: 241 HSPYHRVMMSGNEVRYSTGLFSIPKEGYIIKAPEEVVDEEHPLLFKPFDHVQFLGFYYSE 300

Query: 301 AGQKAQSALKTYCG 313
           AGQ+A SALKTYCG
Sbjct: 301 AGQRAHSALKTYCG 313

[Close]
BLAST of CSPI04G00590 vs. NCBI nr
Match: gi|645244876|ref|XP_008228619.1| (PREDICTED: probable 2-oxoglutarate-dependent dioxygenase AOP1 [Prunus mume])

HSP 1 Score: 483.0 bits (1242), Expect = 3.9e-133
Identity = 223/314 (71.02%), Postives = 272/314 (86.62%), Query Frame = 1 

Query: 1   MDSESSLKLPLIDFSN--LESGGPKWELAKAQVKEALEEFGCFEASFDKVPVEVRKGLFE 60
           M SE+  KLP+IDFSN  L  G  +W   +AQV  ALEE+GCFEA F+KVP  +RK +F 
Sbjct: 1   MGSETPPKLPVIDFSNQSLTPGTTEWNAVRAQVHNALEEYGCFEALFNKVPSHIRKSIFP 60

Query: 61  ALEELFNLPLETKLRNVSQKPFHGYVGQYPMAPLFESMGVDDSTIPQKVQDFTNILWPQG 120
           ++EELF+LPL+TKL+NVS+KP+HGYVGQYPM PL+ESMGVD + + ++VQ  TN LWP+G
Sbjct: 61  SIEELFDLPLQTKLQNVSKKPYHGYVGQYPMVPLYESMGVDGANVYEQVQSLTNTLWPRG 120

Query: 121 NPTFSKVMETYSQQLAELDEMVRRMVLESLGVEKYLEEHLESTNYLLRVMKYKGTESNYE 180
           NP+F K ++++S+Q++ELD+++RRM+LESLG+EKYLEEHLESTNYLLRVMKYKG ++N E
Sbjct: 121 NPSFCKAIQSFSEQVSELDQIIRRMILESLGLEKYLEEHLESTNYLLRVMKYKGPQTN-E 180

Query: 181 TKIGLHSHTDKNIVTILYQNHVQGLQVKTKDGKWINFQPSPDSFVAMIGDSFHAWTNGRL 240
           TK+GL++HTDKNIVTILYQN V GL+V+ KDGKWIN +PSPDSF+AMIGDS +AWTNGRL
Sbjct: 181 TKLGLNAHTDKNIVTILYQNQVDGLEVQNKDGKWINVKPSPDSFIAMIGDSLYAWTNGRL 240

Query: 241 HSPYHRVMMSGEEVRYSAGLFSIPKAGYIVKAPEELVDEQHPLLFNPFDHVQFLQFYYTE 300
           HSPYHRVMMSG E RYS GLFSIPK GYI+KAPEE+VDE+HPLLF PFDHVQFL FYY+E
Sbjct: 241 HSPYHRVMMSGNEARYSTGLFSIPKEGYIIKAPEEVVDEEHPLLFKPFDHVQFLGFYYSE 300

Query: 301 AGQKAQSALKTYCG 313
           AGQ+A SALKTYCG
Sbjct: 301 AGQRAHSALKTYCG 313

[Close]
BLAST of CSPI04G00590 vs. NCBI nr
Match: gi|225442747|ref|XP_002284968.1| (PREDICTED: probable 2-oxoglutarate-dependent dioxygenase AOP1 [Vitis vinifera])

HSP 1 Score: 481.9 bits (1239), Expect = 8.7e-133
Identity = 222/314 (70.70%), Postives = 275/314 (87.58%), Query Frame = 1 

Query: 1   MDSESSLKLPLIDFSN--LESGGPKWELAKAQVKEALEEFGCFEASFDKVPVEVRKGLFE 60
           M SE+ LKLP+IDFS+  L+ G  +W L KAQV++ALEE+GCFEA F+KVP+++RK +F 
Sbjct: 1   MGSETPLKLPVIDFSDPELKPGTLQWNLVKAQVQKALEEYGCFEAFFNKVPLDLRKAIFG 60

Query: 61  ALEELFNLPLETKLRNVSQKPFHGYVGQYPMAPLFESMGVDDSTIPQKVQDFTNILWPQG 120
           ALEELF+LPL+TKLRN S+KP+HGYVGQYP  PL+ESMG+DD+ + +K + FT +LWP+G
Sbjct: 61  ALEELFDLPLQTKLRNSSKKPYHGYVGQYPTVPLYESMGIDDALVQEKAEHFTKLLWPEG 120

Query: 121 NPTFSKVMETYSQQLAELDEMVRRMVLESLGVEKYLEEHLESTNYLLRVMKYKGTESNYE 180
           NP F K ++++S+QL+ELD  +RRMVLESLG+EKYL+EH++STNYLLRVMKYKG ++  E
Sbjct: 121 NPNFCKTIQSFSEQLSELDHTIRRMVLESLGMEKYLDEHMDSTNYLLRVMKYKGPQTT-E 180

Query: 181 TKIGLHSHTDKNIVTILYQNHVQGLQVKTKDGKWINFQPSPDSFVAMIGDSFHAWTNGRL 240
           TK+GL++HTDKNIVTILYQN V GL V+TKDG+WIN + SPDSF+ MIGDSF AWTNGRL
Sbjct: 181 TKLGLNAHTDKNIVTILYQNQVDGLGVQTKDGQWINIKCSPDSFIIMIGDSFLAWTNGRL 240

Query: 241 HSPYHRVMMSGEEVRYSAGLFSIPKAGYIVKAPEELVDEQHPLLFNPFDHVQFLQFYYTE 300
           HSPYHRVMM+G E RYS GLFSIPKAGY++KAPEELVDE+HPLLF PFDH++FLQFYYTE
Sbjct: 241 HSPYHRVMMTGNESRYSTGLFSIPKAGYVIKAPEELVDEEHPLLFKPFDHLEFLQFYYTE 300

Query: 301 AGQKAQSALKTYCG 313
           AGQ+A+SALKTYCG
Sbjct: 301 AGQRAESALKTYCG 313

The following BLAST results are available for this feature:
BLAST of CSPI04G00590 vs. Swiss-Prot
Match NameE-valueIdentityDescription
AOP1L_ARATH1.7e-9956.41Probable 2-oxoglutarate-dependent dioxygenase AOP1.2 OS=Arabidopsis thaliana GN=... [more]
AOP1C_ARATH8.2e-9955.91Probable 2-oxoglutarate-dependent dioxygenase AOP1 OS=Arabidopsis thaliana GN=AO... [more]
AOP1V_ARATH5.0e-9656.29Probable 2-oxoglutarate-dependent dioxygenase AOP1 (Fragment) OS=Arabidopsis tha... [more]
AOP2V_ARATH3.9e-4047.342-oxoglutarate-dependent dioxygenase AOP2 OS=Arabidopsis thaliana GN=AOP2 PE=2 S... [more]
AOP2C_ARATH1.1e-3948.28Probable inactive 2-oxoglutarate-dependent dioxygenase AOP2 OS=Arabidopsis thali... [more]
BLAST of CSPI04G00590 vs. TrEMBL
Match NameE-valueIdentityDescription
M5WXX0_PRUPE3.4e-13672.61Uncharacterized protein OS=Prunus persica GN=PRUPE_ppa021358mg PE=3 SV=1[more]
D7U0Q6_VITVI6.1e-13370.70Putative uncharacterized protein OS=Vitis vinifera GN=VIT_09s0002g05280 PE=3 SV=... [more]
K7KZF3_SOYBN1.3e-13269.11Uncharacterized protein OS=Glycine max GN=GLYMA_07G033700 PE=3 SV=1[more]
A5AJH9_VITVI1.8e-13270.70Putative uncharacterized protein OS=Vitis vinifera GN=VITISV_028512 PE=3 SV=1[more]
A0A0B2NW45_GLYSO5.1e-13268.79Gibberellin 20 oxidase 1 OS=Glycine soja GN=glysoja_009567 PE=3 SV=1[more]
BLAST of CSPI04G00590 vs. TAIR10
Match NameE-valueIdentityDescription
AT1G52820.13.1e-12868.18 2-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily prot... [more]
AT4G03070.14.6e-10055.91 2-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily prot... [more]
AT1G52800.11.0e-7845.45 2-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily prot... [more]
AT1G52790.16.5e-7844.37 2-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily prot... [more]
AT1G28030.12.1e-7646.84 2-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily prot... [more]
BLAST of CSPI04G00590 vs. NCBI nr
Match NameE-valueIdentityDescription
gi|449469106|ref|XP_004152262.1|4.5e-182100.00PREDICTED: probable 2-oxoglutarate-dependent dioxygenase AOP1 [Cucumis sativus][more]
gi|659108754|ref|XP_008454372.1|6.4e-17695.86PREDICTED: probable 2-oxoglutarate-dependent dioxygenase AOP1 [Cucumis melo][more]
gi|595960175|ref|XP_007216862.1|4.9e-13672.61hypothetical protein PRUPE_ppa021358mg [Prunus persica][more]
gi|645244876|ref|XP_008228619.1|3.9e-13371.02PREDICTED: probable 2-oxoglutarate-dependent dioxygenase AOP1 [Prunus mume][more]
gi|225442747|ref|XP_002284968.1|8.7e-13370.70PREDICTED: probable 2-oxoglutarate-dependent dioxygenase AOP1 [Vitis vinifera][more]
Annotated Terms
The following terms have been associated with this gene:
Vocabulary: INTERPRO
TermDefinition
IPR002283Isopenicillin N synthase
IPR005123Oxoglu/Fe-dep_dioxygenase
IPR026992DIOX_N
IPR027443IPNS-like
Vocabulary: Molecular Function
TermDefinition
GO:0005506iron ion binding
GO:0016491oxidoreductase activity
Vocabulary: Biological Process
TermDefinition
GO:0055114oxidation-reduction process
GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0016102 diterpenoid biosynthetic process
biological_process GO:0055114 oxidation-reduction process
cellular_component GO:0005575 cellular_component
molecular_function GO:0016707 gibberellin 3-beta-dioxygenase activity
molecular_function GO:0005506 iron ion binding
molecular_function GO:0016491 oxidoreductase activity
molecular_function GO:0016706 oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen, 2-oxoglutarate as one donor, and incorporation of one atom each of oxygen into both donors
molecular_function GO:0051213 dioxygenase activity
molecular_function GO:0046872 metal ion binding
Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
CSPI04G00590.1CSPI04G00590.1mRNA


InterPro
Analysis Name: InterPro Annotations of cucumber (PI183967)
Date Performed: 2017-01-17
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR002283Isopenicillin N synthasePRINTSPR00682IPNSYNTHASEcoord: 222..248
score: 8.6E-6coord: 186..207
score: 8.
IPR005123Oxoglutarate/iron-dependent dioxygenasePFAMPF031712OG-FeII_Oxycoord: 170..259
score: 2.8
IPR005123Oxoglutarate/iron-dependent dioxygenasePROFILEPS51471FE2OG_OXYcoord: 160..262
score: 10
IPR026992Non-haem dioxygenase N-terminal domainPFAMPF14226DIOX_Ncoord: 9..85
score: 1.2
IPR027443Isopenicillin N synthase-likeGENE3DG3DSA:2.60.120.330coord: 4..303
score: 7.8
NoneNo IPR availableunknownCoilCoilcoord: 126..146
scor
NoneNo IPR availablePANTHERPTHR10209OXIDOREDUCTASE, 2OG-FE II OXYGENASE FAMILY PROTEINcoord: 1..305
score: 7.4E
NoneNo IPR availablePANTHERPTHR10209:SF2112-OXOGLUTARATE (2OG) AND FE(II)-DEPENDENT OXYGENASE SUPERFAMILY PROTEIN-RELATEDcoord: 1..305
score: 7.4E
NoneNo IPR availableunknownSSF51197Clavaminate synthase-likecoord: 5..300
score: 7.55