Lsi03G009970 (gene) Bottle gourd (USVL1VR-Ls) | Cucurbit Genomics Database (CuGenDB)

Overview


Name	Lsi03G009970
Type	gene
Organism	Lagenaria siceraria (Bottle gourd (USVL1VR-Ls))
Description	Cysteine-rich venom protein
Location	chr03 : 19231417 .. 19231917 (+)

Genome Viewer

Gene Expression

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: CDS

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGCGGACTCAAAAGCCTTTCATTATTGCCATTCTCTTGGCCACTTCCATCTTTATCCTAAGCCAAACACATGCTCAAAACTCACCTCAAGACTATGTTACACTACACAACCGTGCTCGAGCCGAAGTTGGCGTCGGCCCCGTTACATGGAACGAGACAGTGGCTGCCTACGCTCAAGCCTATGCGAACCAAAGGAGGAAGGACTGCATCATGGTTAACTCAGGCGGGCCATATGGCGAAAACATACTCGCTGGCTATTTCCCTGAAATCAGTGGAGCATTTGCAGTGAAGTTTTGGTTAGATGAGAAGCGTTTATACAATCCTACGTCGAACACGTGCGTGAAGGGTGAGTGTAAACATTATACCCAAGTGGTGTGGCGAAACTCAGTGCAGATTGGATGTGCTAGAGTTTTATGTAACACTAATTCTCAATTTGTTATATGTAATTATCATCCTCCTGGCAATATTGTTGGAGAAAGACCTTATGATTCTTCAATCTAA

mRNA sequence

ATGCGGACTCAAAAGCCTTTCATTATTGCCATTCTCTTGGCCACTTCCATCTTTATCCTAAGCCAAACACATGCTCAAAACTCACCTCAAGACTATGTTACACTACACAACCGTGCTCGAGCCGAAGTTGGCGTCGGCCCCGTTACATGGAACGAGACAGTGGCTGCCTACGCTCAAGCCTATGCGAACCAAAGGAGGAAGGACTGCATCATGGTTAACTCAGGCGGGCCATATGGCGAAAACATACTCGCTGGCTATTTCCCTGAAATCAGTGGAGCATTTGCAGTGAAGTTTTGGTTAGATGAGAAGCGTTTATACAATCCTACGTCGAACACGTGCGTGAAGGGTGAGTGTAAACATTATACCCAAGTGGTGTGGCGAAACTCAGTGCAGATTGGATGTGCTAGAGTTTTATGTAACACTAATTCTCAATTTGTTATATGTAATTATCATCCTCCTGGCAATATTGTTGGAGAAAGACCTTATGATTCTTCAATCTAA

Coding sequence (CDS)

ATGCGGACTCAAAAGCCTTTCATTATTGCCATTCTCTTGGCCACTTCCATCTTTATCCTAAGCCAAACACATGCTCAAAACTCACCTCAAGACTATGTTACACTACACAACCGTGCTCGAGCCGAAGTTGGCGTCGGCCCCGTTACATGGAACGAGACAGTGGCTGCCTACGCTCAAGCCTATGCGAACCAAAGGAGGAAGGACTGCATCATGGTTAACTCAGGCGGGCCATATGGCGAAAACATACTCGCTGGCTATTTCCCTGAAATCAGTGGAGCATTTGCAGTGAAGTTTTGGTTAGATGAGAAGCGTTTATACAATCCTACGTCGAACACGTGCGTGAAGGGTGAGTGTAAACATTATACCCAAGTGGTGTGGCGAAACTCAGTGCAGATTGGATGTGCTAGAGTTTTATGTAACACTAATTCTCAATTTGTTATATGTAATTATCATCCTCCTGGCAATATTGTTGGAGAAAGACCTTATGATTCTTCAATCTAA

Protein sequence

MRTQKPFIIAILLATSIFILSQTHAQNSPQDYVTLHNRARAEVGVGPVTWNETVAAYAQAYANQRRKDCIMVNSGGPYGENILAGYFPEISGAFAVKFWLDEKRLYNPTSNTCVKGECKHYTQVVWRNSVQIGCARVLCNTNSQFVICNYHPPGNIVGERPYDSSI

Homology

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BLAST of Lsi03G009970 vs. Swiss-Prot
Match: PRB1_TOBAC (Basic form of pathogenesis-related protein 1 OS=Nicotiana tabacum PE=3 SV=1)

HSP 1 Score: 196.8 bits (499), Expect = 1.8e-49
Identity = 92/155 (59.35%), Postives = 115/155 (74.19%), Query Frame = 1


Query: 8   IIAILLATSIFILSQTHAQNSPQDYVTLHNRARAEVGVGPVTWNETVAAYAQAYANQRRK 67
           I+A  +  +I I S + AQNSPQDY+  HN AR +VGVGP+TW+  +AAYAQ YANQR  
Sbjct: 7   IVACFITFAILIHS-SKAQNSPQDYLNPHNAARRQVGVGPMTWDNRLAAYAQNYANQRIG 66

Query: 68  DCIMVNSGGPYGENILAGYFPEISGAFAVKFWLDEKRLYNPTSNTCVKGECKHYTQVVWR 127
           DC M++S GPYGEN LA  FP+++ A AVK W+DEKR Y+  SN+CV G C HYTQVVWR
Sbjct: 67  DCGMIHSHGPYGEN-LAAAFPQLNAAGAVKMWVDEKRFYDYNSNSCVGGVCGHYTQVVWR 126

Query: 128 NSVQIGCARVLCNTNSQFVICNYHPPGNIVGERPY 163
           NSV++GCARV  N    F+ CNY PPGN +G+RP+
Sbjct: 127 NSVRLGCARVRSNNGWFFITCNYDPPGNFIGQRPF 159

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BLAST of Lsi03G009970 vs. Swiss-Prot
Match: PR1_SAMNI (Pathogenesis-related protein PR-1 type OS=Sambucus nigra PE=2 SV=1)

HSP 1 Score: 187.6 bits (475), Expect = 1.1e-46
Identity = 86/158 (54.43%), Postives = 112/158 (70.89%), Query Frame = 1


Query: 7   FIIAILLATSIFILSQTHAQNSPQDYVTLHNRARAEVGVGPVTWNETVAAYAQAYANQRR 66
           F +A++   ++ ++  + AQNSPQDYV  HN AR+ V VGPVTW+E+VAA+A+ YA  R 
Sbjct: 10  FSVALVCVVALVMVQYSVAQNSPQDYVDAHNAARSAVNVGPVTWDESVAAFARQYAQSRA 69

Query: 67  KDCIMVNSGGP-YGENILAGYFPEISGAFAVKFWLDEKRLYNPTSNTCVKGE-CKHYTQV 126
            DC +V+SG P YGEN+  G   E++G  AV  W+ E+  YNP +NTC  G+ C HYTQV
Sbjct: 70  GDCRLVHSGDPRYGENLAFGSGFELTGRNAVDMWVAERNDYNPNTNTCAPGKVCGHYTQV 129

Query: 127 VWRNSVQIGCARVLCNTNSQFVICNYHPPGNIVGERPY 163
           VWRNSV+IGCARV CN  + F+ CNY PPGN  G+RPY
Sbjct: 130 VWRNSVRIGCARVRCNNGAWFITCNYSPPGNYAGQRPY 167

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BLAST of Lsi03G009970 vs. Swiss-Prot
Match: PR1B_TOBAC (Pathogenesis-related protein 1B OS=Nicotiana tabacum PE=2 SV=1)

HSP 1 Score: 182.2 bits (461), Expect = 4.7e-45
Identity = 83/157 (52.87%), Postives = 107/157 (68.15%), Query Frame = 1


Query: 7   FIIAILLATSIFILSQTHAQNSPQDYVTLHNRARAEVGVGPVTWNETVAAYAQAYANQRR 66
           F +   L   + I   +HAQNS QDY+  HN ARA+VGV P+TW+  VAAYAQ Y +Q  
Sbjct: 12  FFLVSTLLLFLIISHSSHAQNSQQDYLDAHNTARADVGVEPLTWDNGVAAYAQNYVSQLA 71

Query: 67  KDCIMVNSGGPYGENILAGYFPEISGAFAVKFWLDEKRLYNPTSNTCVKGE-CKHYTQVV 126
            DC +V+S G YGEN+  G    ++ A AV+ W+DEK+ Y+  SNTC +G+ C HYTQVV
Sbjct: 72  ADCNLVHSHGQYGENLAQGSGDFMTAAKAVEMWVDEKQYYDHDSNTCAQGQVCGHYTQVV 131

Query: 127 WRNSVQIGCARVLCNTNSQFVICNYHPPGNIVGERPY 163
           WRNSV++GCARV CN     V CNY PPGN++G+ PY
Sbjct: 132 WRNSVRVGCARVKCNNGGYVVSCNYDPPGNVIGQSPY 168

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BLAST of Lsi03G009970 vs. Swiss-Prot
Match: PR1C_TOBAC (Pathogenesis-related protein 1C OS=Nicotiana tabacum PE=2 SV=3)

HSP 1 Score: 180.6 bits (457), Expect = 1.4e-44
Identity = 83/157 (52.87%), Postives = 107/157 (68.15%), Query Frame = 1


Query: 7   FIIAILLATSIFILSQTHAQNSPQDYVTLHNRARAEVGVGPVTWNETVAAYAQAYANQRR 66
           F +   L   + I    HAQNS QDY+  HN ARA+VGV P+TW++ VAAYAQ YA+Q  
Sbjct: 12  FFLVSTLLLFLIISHSCHAQNSQQDYLDAHNTARADVGVEPLTWDDQVAAYAQNYASQLA 71

Query: 67  KDCIMVNSGGPYGENILAGYFPEISGAFAVKFWLDEKRLYNPTSNTCVKGE-CKHYTQVV 126
            DC +V+S G YGEN+  G    ++ A AV+ W++EK+ Y   SNTC +G+ C HYTQVV
Sbjct: 72  ADCNLVHSHGQYGENLAWGSGDFLTAAKAVEMWVNEKQYYAHDSNTCAQGQVCGHYTQVV 131

Query: 127 WRNSVQIGCARVLCNTNSQFVICNYHPPGNIVGERPY 163
           WRNSV++GCARV CN     V CNY PPGN++G+ PY
Sbjct: 132 WRNSVRVGCARVQCNNGGYIVSCNYDPPGNVIGKSPY 168

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BLAST of Lsi03G009970 vs. Swiss-Prot
Match: PR1A_TOBAC (Pathogenesis-related protein 1A OS=Nicotiana tabacum PE=1 SV=1)

HSP 1 Score: 178.7 bits (452), Expect = 5.2e-44
Identity = 84/157 (53.50%), Postives = 106/157 (67.52%), Query Frame = 1


Query: 7   FIIAILLATSIFILSQTHAQNSPQDYVTLHNRARAEVGVGPVTWNETVAAYAQAYANQRR 66
           F++   L   + I     AQNS QDY+  HN ARA+VGV P+TW++ VAAYAQ YA+Q  
Sbjct: 12  FLLVSTLLLFLVISHSCRAQNSQQDYLDAHNTARADVGVEPLTWDDQVAAYAQNYASQLA 71

Query: 67  KDCIMVNSGGPYGENILAGYFPEISGAFAVKFWLDEKRLYNPTSNTCVKGE-CKHYTQVV 126
            DC +V+S G YGEN+  G    ++ A AV+ W+DEK+ Y+  SNTC +G+ C HYTQVV
Sbjct: 72  ADCNLVHSHGQYGENLAEGSGDFMTAAKAVEMWVDEKQYYDHDSNTCAQGQVCGHYTQVV 131

Query: 127 WRNSVQIGCARVLCNTNSQFVICNYHPPGNIVGERPY 163
           WRNSV++GCARV CN     V CNY PPGN  GE PY
Sbjct: 132 WRNSVRVGCARVQCNNGGYVVSCNYDPPGNYRGESPY 168

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BLAST of Lsi03G009970 vs. TrEMBL
Match: A0A0A0KHP4_CUCSA (Uncharacterized protein OS=Cucumis sativus GN=Csa_6G483242 PE=3 SV=1)

HSP 1 Score: 225.7 bits (574), Expect = 4.1e-56
Identity = 96/150 (64.00%), Postives = 120/150 (80.00%), Query Frame = 1


Query: 17  IFILSQTHAQNSPQDYVTLHNRARAEVGVGPVTWNETVAAYAQAYANQRRKDCIMVNSGG 76
           +F+   THAQNSPQDY++LHN+ARA VGVGP+TW+ TVAAYAQ+YAN+R+ DC +V+S G
Sbjct: 1   MFLFVLTHAQNSPQDYISLHNKARAAVGVGPMTWDNTVAAYAQSYANKRKNDCALVHSTG 60

Query: 77  PYGENILAGYFPEISGAFAVKFWLDEKRLYNPTSNTCVKGECKHYTQVVWRNSVQIGCAR 136
           PYGENI  G++PE +GA  VK W+ EK LY+  SN+C  G+C HYTQVVWR SV +GCAR
Sbjct: 61  PYGENIAIGFYPEFTGADGVKLWVGEKHLYDYASNSCKGGDCGHYTQVVWRTSVHLGCAR 120

Query: 137 VLCNTNSQFVICNYHPPGNIVGERPYDSSI 167
           V C   SQFV+CNY PPGN +G RPY+SS+
Sbjct: 121 VPCKGKSQFVVCNYDPPGNYIGLRPYNSSM 150

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BLAST of Lsi03G009970 vs. TrEMBL
Match: A0A0A0LSG5_CUCSA (Uncharacterized protein OS=Cucumis sativus GN=Csa_2G381380 PE=3 SV=1)

HSP 1 Score: 224.9 bits (572), Expect = 7.0e-56
Identity = 98/158 (62.03%), Postives = 121/158 (76.58%), Query Frame = 1


Query: 9   IAILLATSIFILSQTHAQNSPQDYVTLHNRARAEVGVGPVTWNETVAAYAQAYANQRRKD 68
           +AI   T  F+   T AQ SPQDY++LHN+ARA V VGP+TWN+TVAAYAQ+YAN+R+ D
Sbjct: 1   MAIFFPTFTFLFFLTQAQKSPQDYISLHNKARAAVRVGPMTWNKTVAAYAQSYANKRKND 60

Query: 69  CIMVNSGGPYGENILAGYFPEISGAFAVKFWLDEKRLYNPTSNTCVKGECKHYTQVVWRN 128
           C +V+S GPYGENI  GY+PE +GA  VK W+ EK LY+  SN+C  G+C HYTQVVWR 
Sbjct: 61  CALVHSTGPYGENIAVGYYPEFTGADGVKLWVGEKHLYDYASNSCKGGDCGHYTQVVWRT 120

Query: 129 SVQIGCARVLCNTNSQFVICNYHPPGNIVGERPYDSSI 167
           SV +GCARV C   SQFV+CNY PPGN +G RPY+SS+
Sbjct: 121 SVHLGCARVACKGKSQFVVCNYDPPGNYIGLRPYNSSM 158

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BLAST of Lsi03G009970 vs. TrEMBL
Match: E2GEW0_9ROSI (Pathogenesis-related protein 1 OS=Vitis hybrid cultivar GN=PR-1 PE=3 SV=1)

HSP 1 Score: 206.5 bits (524), Expect = 2.6e-50
Identity = 98/155 (63.23%), Postives = 116/155 (74.84%), Query Frame = 1


Query: 9   IAILLATSIFILSQTH-AQNSPQDYVTLHNRARAEVGVGPVTWNETVAAYAQAYANQRRK 68
           +A+L+    F L+ T  AQNSPQDYV  HN ARA+VGVGP+TWN+TVAAYAQ YANQR  
Sbjct: 8   LALLVGLMGFALAHTCCAQNSPQDYVDPHNAARAQVGVGPITWNDTVAAYAQNYANQRIG 67

Query: 69  DCIMVNSGGPYGENILAGYFPEISGAFAVKFWLDEKRLYNPTSNTCVKGECKHYTQVVWR 128
           DC +V+SGGPYGENI  G  P ++G  AV  W+ EK  Y+  SN+CV G+C HYTQVVWR
Sbjct: 68  DCNLVHSGGPYGENIAWGS-PSLTGTDAVNLWVGEKPNYDYNSNSCVGGKCGHYTQVVWR 127

Query: 129 NSVQIGCARVLCNTNSQFVICNYHPPGNIVGERPY 163
           NSV++GCARV CN    FV CNY PPGN VG+RPY
Sbjct: 128 NSVRLGCARVQCNNGGWFVTCNYDPPGNYVGQRPY 161

[Close]

BLAST of Lsi03G009970 vs. TrEMBL
Match: E2GEV6_9ROSI (Pathogenesis-related protein 1 OS=Vitis hybrid cultivar GN=PR-1 PE=3 SV=1)

HSP 1 Score: 206.5 bits (524), Expect = 2.6e-50
Identity = 98/155 (63.23%), Postives = 116/155 (74.84%), Query Frame = 1


Query: 9   IAILLATSIFILSQTH-AQNSPQDYVTLHNRARAEVGVGPVTWNETVAAYAQAYANQRRK 68
           +A+L+    F L+ T  AQNSPQDYV  HN ARA+VGVGP+TWN+TVAAYAQ YANQR  
Sbjct: 8   LALLVGLMGFALAHTCCAQNSPQDYVDSHNAARAQVGVGPITWNDTVAAYAQNYANQRIG 67

Query: 69  DCIMVNSGGPYGENILAGYFPEISGAFAVKFWLDEKRLYNPTSNTCVKGECKHYTQVVWR 128
           DC +V+SGGPYGENI  G  P ++G  AV  W+ EK  Y+  SN+CV GEC HYTQVVWR
Sbjct: 68  DCNLVHSGGPYGENIAWGS-PSLTGTDAVNMWVGEKPNYDYNSNSCVGGECGHYTQVVWR 127

Query: 129 NSVQIGCARVLCNTNSQFVICNYHPPGNIVGERPY 163
           NSV++GCARV C+    FV CNY PPGN VG+RPY
Sbjct: 128 NSVRLGCARVQCDNGGWFVTCNYDPPGNYVGQRPY 161

[Close]

BLAST of Lsi03G009970 vs. TrEMBL
Match: E2GEV8_9ROSI (Pathogenesis-related protein 1 OS=Vitis hybrid cultivar GN=PR-1 PE=3 SV=1)

HSP 1 Score: 204.5 bits (519), Expect = 9.8e-50
Identity = 96/155 (61.94%), Postives = 116/155 (74.84%), Query Frame = 1


Query: 9   IAILLATSIFILSQTH-AQNSPQDYVTLHNRARAEVGVGPVTWNETVAAYAQAYANQRRK 68
           +A+L+    F L+ T  AQNSPQDYV  HN A+A+VGVGP+TWN+TVAAYAQ YANQR  
Sbjct: 8   LALLVGLMGFALAHTCCAQNSPQDYVDPHNAAQAQVGVGPITWNDTVAAYAQNYANQRIG 67

Query: 69  DCIMVNSGGPYGENILAGYFPEISGAFAVKFWLDEKRLYNPTSNTCVKGECKHYTQVVWR 128
           DC +V+SGGPYGENI  G  P ++G  AV  W+ EK  Y+  SN+CV G+C HYTQVVWR
Sbjct: 68  DCNLVHSGGPYGENIAWGS-PSLTGTDAVNMWVGEKPNYDYNSNSCVGGKCGHYTQVVWR 127

Query: 129 NSVQIGCARVLCNTNSQFVICNYHPPGNIVGERPY 163
           NSV++GCARV CN    F+ CNY PPGN VG+RPY
Sbjct: 128 NSVRLGCARVQCNNGGWFITCNYDPPGNYVGQRPY 161

[Close]

BLAST of Lsi03G009970 vs. TAIR10
Match: AT4G33720.1 (AT4G33720.1 CAP (Cysteine-rich secretory proteins, Antigen 5, and Pathogenesis-related 1 protein) superfamily protein)

HSP 1 Score: 178.3 bits (451), Expect = 3.8e-45
Identity = 86/155 (55.48%), Postives = 109/155 (70.32%), Query Frame = 1


Query: 11  ILLATSIFILSQTH--AQNSPQDYVTLHNRARAEVGVGPVTWNETVAAYAQAYANQRRKD 70
           + LA + F++   H  AQ+SPQD++ +HNRARAEVGVGP+ W+E VAAYA+ YANQR+ D
Sbjct: 10  LFLAITFFLVLIVHLKAQDSPQDFLAVHNRARAEVGVGPLRWDEKVAAYARNYANQRKGD 69

Query: 71  CIMVNSGGPYGENILAGYFPEISGAFAVKFWLDEKRLYNPTSNTCV-KGECKHYTQVVWR 130
           C M +S G YGENI A     ++G  AV  W+DE+  Y+  SNTC    +C HYTQVVWR
Sbjct: 70  CAMKHSSGSYGENI-AWSSGSMTGVAAVDMWVDEQFDYDYDSNTCAWDKQCGHYTQVVWR 129

Query: 131 NSVQIGCARVLCNTNSQFVICNYHPPGNIVGERPY 163
           NS ++GCA+V CN    F+ CNY PPGN VGE PY
Sbjct: 130 NSERLGCAKVRCNNGQTFITCNYDPPGNWVGEWPY 163

[Close]

BLAST of Lsi03G009970 vs. TAIR10
Match: AT1G50060.1 (AT1G50060.1 CAP (Cysteine-rich secretory proteins, Antigen 5, and Pathogenesis-related 1 protein) superfamily protein)

HSP 1 Score: 176.8 bits (447), Expect = 1.1e-44
Identity = 83/158 (52.53%), Postives = 107/158 (67.72%), Query Frame = 1


Query: 6   PFIIAILLATSIFILSQTHAQNSPQDYVTLHNRARAEVGVGPVTWNETVAAYAQAYANQR 65
           PF++ + ++   F++  T+AQN+PQDY+  HN ARA+VGV  V W+ T+AAYA  Y+N R
Sbjct: 7   PFLVIVAIS---FLVVATNAQNTPQDYLNSHNTARAQVGVPNVVWDTTLAAYALNYSNFR 66

Query: 66  RKDCIMVNSGGPYGENILAGYFPEISGAFAVKFWLDEKRLYNPTSNTCVKG-ECKHYTQV 125
           + DC +V+S GPYGEN+  G     S   AVK W+DEK  Y+   N C  G +C HYTQV
Sbjct: 67  KADCNLVHSNGPYGENLAKGSSSSFSAISAVKLWVDEKPYYSYAYNNCTGGKQCLHYTQV 126

Query: 126 VWRNSVQIGCARVLCNTNSQFVICNYHPPGNIVGERPY 163
           VWR+SV+IGCARV C     FV CNY+ PGN VGE PY
Sbjct: 127 VWRDSVKIGCARVQCTNTWWFVSCNYNSPGNWVGEYPY 161

[Close]

BLAST of Lsi03G009970 vs. TAIR10
Match: AT2G14610.1 (AT2G14610.1 pathogenesis-related gene 1)

HSP 1 Score: 173.3 bits (438), Expect = 1.2e-43
Identity = 80/156 (51.28%), Postives = 106/156 (67.95%), Query Frame = 1


Query: 7   FIIAILLATSIFILSQTHAQNSPQDYVTLHNRARAEVGVGPVTWNETVAAYAQAYANQRR 66
           F+I  +      +L  + AQ+SPQDY+ +HN+AR  VGVGP+ W+E VAAYA++YA Q R
Sbjct: 9   FLIVFVALVGALVLP-SKAQDSPQDYLRVHNQARGAVGVGPMQWDERVAAYARSYAEQLR 68

Query: 67  KDCIMVNSGGPYGENILAGYFPEISGAFAVKFWLDEKRLYNPTSNTCVKGECKHYTQVVW 126
            +C +++SGGPYGEN+  G   ++SG  AV  W+ EK  YN  +NTC  G C HYTQVVW
Sbjct: 69  GNCRLIHSGGPYGENLAWG-SGDLSGVSAVNMWVSEKANYNYAANTC-NGVCGHYTQVVW 128

Query: 127 RNSVQIGCARVLCNTNSQFVICNYHPPGNIVGERPY 163
           R SV++GCA+V CN     + CNY P GN V E+PY
Sbjct: 129 RKSVRLGCAKVRCNNGGTIISCNYDPRGNYVNEKPY 161

[Close]

BLAST of Lsi03G009970 vs. TAIR10
Match: AT2G14580.1 (AT2G14580.1 basic pathogenesis-related protein 1)

HSP 1 Score: 172.6 bits (436), Expect = 2.1e-43
Identity = 81/155 (52.26%), Postives = 107/155 (69.03%), Query Frame = 1


Query: 8   IIAILLATSIFILSQTHAQNSPQDYVTLHNRARAEVGVGPVTWNETVAAYAQAYANQRRK 67
           I+ IL A    ++    AQ+S QDYV  HN+AR+++GVGP+ W+E +AAYA+ YANQ + 
Sbjct: 9   ILIILAALVGALVVPLKAQDSQQDYVNAHNQARSQIGVGPMQWDEGLAAYARNYANQLKG 68

Query: 68  DCIMVNSGGPYGENILAGYFPEISGAFAVKFWLDEKRLYNPTSNTCVKGECKHYTQVVWR 127
           DC +V+S GPYGEN LA    ++SG  AV  W++EK  YN  +NTC  G C HYTQVVWR
Sbjct: 69  DCRLVHSRGPYGEN-LAKSGGDLSGVAAVNLWVNEKANYNYDTNTC-NGVCGHYTQVVWR 128

Query: 128 NSVQIGCARVLCNTNSQFVICNYHPPGNIVGERPY 163
           NSV++GCA+V CN     + CNY PPGN   ++PY
Sbjct: 129 NSVRLGCAKVRCNNGGTIISCNYDPPGNYANQKPY 161

[Close]

BLAST of Lsi03G009970 vs. TAIR10
Match: AT2G19990.1 (AT2G19990.1 pathogenesis-related protein-1-like)

HSP 1 Score: 164.9 bits (416), Expect = 4.4e-41
Identity = 74/136 (54.41%), Postives = 101/136 (74.26%), Query Frame = 1


Query: 29  PQDYVTLHNRARAEVGVGPVTWNETVAAYAQAYANQRRKDCIMVNSGGPYGENILAGYFP 88
           PQ+ + +HN+ARA VGVGP+ WNET+A YAQ+YA++R +DC M +S GP+GEN+ AG+  
Sbjct: 42  PQETLVVHNKARAMVGVGPMVWNETLATYAQSYAHERARDCAMKHSLGPFGENLAAGW-G 101

Query: 89  EISGAFAVKFWLDEKRLYNPTSNTC-VKGECKHYTQVVWRNSVQIGCARVLC-NTNSQFV 148
            +SG  A ++W+ EK  Y+  SNTC   G C HYTQ+VWR+SV++GCA V C N    +V
Sbjct: 102 TMSGPVATEYWMTEKENYDYDSNTCGGDGVCGHYTQIVWRDSVRLGCASVRCKNDEYIWV 161

Query: 149 ICNYHPPGNIVGERPY 163
           IC+Y PPGN +G+RPY
Sbjct: 162 ICSYDPPGNYIGQRPY 176

[Close]

BLAST of Lsi03G009970 vs. NCBI nr
Match: gi|659109009|ref|XP_008454501.1| (PREDICTED: basic form of pathogenesis-related protein 1-like [Cucumis melo])

HSP 1 Score: 234.6 bits (597), Expect = 1.3e-58
Identity = 102/157 (64.97%), Postives = 125/157 (79.62%), Query Frame = 1


Query: 9   IAILLATSIFILSQTHAQNSPQDYVTLHNRARAEVGVGPVTWNETVAAYAQAYANQRRKD 68
           +AI  A+ +F+   THAQNSPQDY+ LHN+ARA VGVGP+TWN T+AAYA+AYANQR KD
Sbjct: 1   MAIFFASFMFLFFLTHAQNSPQDYIALHNKARAAVGVGPMTWNSTLAAYAEAYANQRIKD 60

Query: 69  CIMVNSGGPYGENILAGYFPEISGAFAVKFWLDEKRLYNPTSNTCVKGECKHYTQVVWRN 128
           C +V+S GPYGENI  GY+PE +GA  VK W+ EKRLY+  SN+C  G+C HYTQ+VWR 
Sbjct: 61  CALVHSKGPYGENIAVGYYPEFTGADGVKLWVAEKRLYDYASNSCKGGDCGHYTQLVWRT 120

Query: 129 SVQIGCARVLCNTNSQFVICNYHPPGNIVGERPYDSS 166
           SV++GCARV C   SQFV+CNY PPGN +G RPY+SS
Sbjct: 121 SVRLGCARVACKGKSQFVVCNYDPPGNYIGLRPYNSS 157

[Close]

BLAST of Lsi03G009970 vs. NCBI nr
Match: gi|778722387|ref|XP_011658476.1| (PREDICTED: basic form of pathogenesis-related protein 1-like [Cucumis sativus])

HSP 1 Score: 230.7 bits (587), Expect = 1.8e-57
Identity = 100/158 (63.29%), Postives = 125/158 (79.11%), Query Frame = 1


Query: 9   IAILLATSIFILSQTHAQNSPQDYVTLHNRARAEVGVGPVTWNETVAAYAQAYANQRRKD 68
           +AIL  T +F+   THAQNSPQDY++LHN+ARA VGVGP+TW+ TVAAYAQ+YAN+R+ D
Sbjct: 1   MAILFPTFMFLFVLTHAQNSPQDYISLHNKARAAVGVGPMTWDNTVAAYAQSYANKRKND 60

Query: 69  CIMVNSGGPYGENILAGYFPEISGAFAVKFWLDEKRLYNPTSNTCVKGECKHYTQVVWRN 128
           C +V+S GPYGENI  G++PE +GA  VK W+ EK LY+  SN+C  G+C HYTQVVWR 
Sbjct: 61  CALVHSTGPYGENIAIGFYPEFTGADGVKLWVGEKHLYDYASNSCKGGDCGHYTQVVWRT 120

Query: 129 SVQIGCARVLCNTNSQFVICNYHPPGNIVGERPYDSSI 167
           SV +GCARV C   SQFV+CNY PPGN +G RPY+SS+
Sbjct: 121 SVHLGCARVPCKGKSQFVVCNYDPPGNYIGLRPYNSSM 158

[Close]

BLAST of Lsi03G009970 vs. NCBI nr
Match: gi|700193135|gb|KGN48339.1| (hypothetical protein Csa_6G483242 [Cucumis sativus])

HSP 1 Score: 225.7 bits (574), Expect = 5.9e-56
Identity = 96/150 (64.00%), Postives = 120/150 (80.00%), Query Frame = 1


Query: 17  IFILSQTHAQNSPQDYVTLHNRARAEVGVGPVTWNETVAAYAQAYANQRRKDCIMVNSGG 76
           +F+   THAQNSPQDY++LHN+ARA VGVGP+TW+ TVAAYAQ+YAN+R+ DC +V+S G
Sbjct: 1   MFLFVLTHAQNSPQDYISLHNKARAAVGVGPMTWDNTVAAYAQSYANKRKNDCALVHSTG 60

Query: 77  PYGENILAGYFPEISGAFAVKFWLDEKRLYNPTSNTCVKGECKHYTQVVWRNSVQIGCAR 136
           PYGENI  G++PE +GA  VK W+ EK LY+  SN+C  G+C HYTQVVWR SV +GCAR
Sbjct: 61  PYGENIAIGFYPEFTGADGVKLWVGEKHLYDYASNSCKGGDCGHYTQVVWRTSVHLGCAR 120

Query: 137 VLCNTNSQFVICNYHPPGNIVGERPYDSSI 167
           V C   SQFV+CNY PPGN +G RPY+SS+
Sbjct: 121 VPCKGKSQFVVCNYDPPGNYIGLRPYNSSM 150

[Close]

BLAST of Lsi03G009970 vs. NCBI nr
Match: gi|700207817|gb|KGN62936.1| (hypothetical protein Csa_2G381380 [Cucumis sativus])

HSP 1 Score: 224.9 bits (572), Expect = 1.0e-55
Identity = 98/158 (62.03%), Postives = 121/158 (76.58%), Query Frame = 1


Query: 9   IAILLATSIFILSQTHAQNSPQDYVTLHNRARAEVGVGPVTWNETVAAYAQAYANQRRKD 68
           +AI   T  F+   T AQ SPQDY++LHN+ARA V VGP+TWN+TVAAYAQ+YAN+R+ D
Sbjct: 1   MAIFFPTFTFLFFLTQAQKSPQDYISLHNKARAAVRVGPMTWNKTVAAYAQSYANKRKND 60

Query: 69  CIMVNSGGPYGENILAGYFPEISGAFAVKFWLDEKRLYNPTSNTCVKGECKHYTQVVWRN 128
           C +V+S GPYGENI  GY+PE +GA  VK W+ EK LY+  SN+C  G+C HYTQVVWR 
Sbjct: 61  CALVHSTGPYGENIAVGYYPEFTGADGVKLWVGEKHLYDYASNSCKGGDCGHYTQVVWRT 120

Query: 129 SVQIGCARVLCNTNSQFVICNYHPPGNIVGERPYDSSI 167
           SV +GCARV C   SQFV+CNY PPGN +G RPY+SS+
Sbjct: 121 SVHLGCARVACKGKSQFVVCNYDPPGNYIGLRPYNSSM 158

[Close]

BLAST of Lsi03G009970 vs. NCBI nr
Match: gi|307340541|gb|ADN43432.1| (pathogenesis-related protein 1 [Vitis hybrid cultivar])

HSP 1 Score: 206.5 bits (524), Expect = 3.7e-50
Identity = 98/155 (63.23%), Postives = 116/155 (74.84%), Query Frame = 1


Query: 9   IAILLATSIFILSQTH-AQNSPQDYVTLHNRARAEVGVGPVTWNETVAAYAQAYANQRRK 68
           +A+L+    F L+ T  AQNSPQDYV  HN ARA+VGVGP+TWN+TVAAYAQ YANQR  
Sbjct: 8   LALLVGLMGFALAHTCCAQNSPQDYVDSHNAARAQVGVGPITWNDTVAAYAQNYANQRIG 67

Query: 69  DCIMVNSGGPYGENILAGYFPEISGAFAVKFWLDEKRLYNPTSNTCVKGECKHYTQVVWR 128
           DC +V+SGGPYGENI  G  P ++G  AV  W+ EK  Y+  SN+CV GEC HYTQVVWR
Sbjct: 68  DCNLVHSGGPYGENIAWGS-PSLTGTDAVNMWVGEKPNYDYNSNSCVGGECGHYTQVVWR 127

Query: 129 NSVQIGCARVLCNTNSQFVICNYHPPGNIVGERPY 163
           NSV++GCARV C+    FV CNY PPGN VG+RPY
Sbjct: 128 NSVRLGCARVQCDNGGWFVTCNYDPPGNYVGQRPY 161

The following BLAST results are available for this feature:

BLAST of Lsi03G009970 vs. Swiss-Prot


Match Name	E-value	Identity	Description
PRB1_TOBAC	1.8e-49	59.35	Basic form of pathogenesis-related protein 1 OS=Nicotiana tabacum PE=3 SV=1	[more]
PR1_SAMNI	1.1e-46	54.43	Pathogenesis-related protein PR-1 type OS=Sambucus nigra PE=2 SV=1	[more]
PR1B_TOBAC	4.7e-45	52.87	Pathogenesis-related protein 1B OS=Nicotiana tabacum PE=2 SV=1	[more]
PR1C_TOBAC	1.4e-44	52.87	Pathogenesis-related protein 1C OS=Nicotiana tabacum PE=2 SV=3	[more]
PR1A_TOBAC	5.2e-44	53.50	Pathogenesis-related protein 1A OS=Nicotiana tabacum PE=1 SV=1	[more]

BLAST of Lsi03G009970 vs. TrEMBL


Match Name	E-value	Identity	Description
A0A0A0KHP4_CUCSA	4.1e-56	64.00	Uncharacterized protein OS=Cucumis sativus GN=Csa_6G483242 PE=3 SV=1	[more]
A0A0A0LSG5_CUCSA	7.0e-56	62.03	Uncharacterized protein OS=Cucumis sativus GN=Csa_2G381380 PE=3 SV=1	[more]
E2GEW0_9ROSI	2.6e-50	63.23	Pathogenesis-related protein 1 OS=Vitis hybrid cultivar GN=PR-1 PE=3 SV=1	[more]
E2GEV6_9ROSI	2.6e-50	63.23	Pathogenesis-related protein 1 OS=Vitis hybrid cultivar GN=PR-1 PE=3 SV=1	[more]
E2GEV8_9ROSI	9.8e-50	61.94	Pathogenesis-related protein 1 OS=Vitis hybrid cultivar GN=PR-1 PE=3 SV=1	[more]

BLAST of Lsi03G009970 vs. TAIR10


Match Name	E-value	Identity	Description
AT4G33720.1	3.8e-45	55.48	CAP (Cysteine-rich secretory proteins, Antigen 5, and Pathogenesis-r...	[more]
AT1G50060.1	1.1e-44	52.53	CAP (Cysteine-rich secretory proteins, Antigen 5, and Pathogenesis-r...	[more]
AT2G14610.1	1.2e-43	51.28	pathogenesis-related gene 1	[more]
AT2G14580.1	2.1e-43	52.26	basic pathogenesis-related protein 1	[more]
AT2G19990.1	4.4e-41	54.41	pathogenesis-related protein-1-like	[more]

BLAST of Lsi03G009970 vs. NCBI nr


Match Name	E-value	Identity	Description
gi\|659109009\|ref\|XP_008454501.1\|	1.3e-58	64.97	PREDICTED: basic form of pathogenesis-related protein 1-like [Cucumis melo]	[more]
gi\|778722387\|ref\|XP_011658476.1\|	1.8e-57	63.29	PREDICTED: basic form of pathogenesis-related protein 1-like [Cucumis sativus]	[more]
gi\|700193135\|gb\|KGN48339.1\|	5.9e-56	64.00	hypothetical protein Csa_6G483242 [Cucumis sativus]	[more]
gi\|700207817\|gb\|KGN62936.1\|	1.0e-55	62.03	hypothetical protein Csa_2G381380 [Cucumis sativus]	[more]
gi\|307340541\|gb\|ADN43432.1\|	3.7e-50	63.23	pathogenesis-related protein 1 [Vitis hybrid cultivar]	[more]

Annotated Terms

The following terms have been associated with this gene:

Vocabulary: **Cellular Component**
Term	Definition
GO:0005576	extracellular region

Vocabulary: **INTERPRO**
Term	Definition
IPR018244	Allrgn_V5/Tpx1_CS
IPR014044	CAP_domain
IPR002413	V5_allergen
IPR001283	Allrgn_V5/Tpx1

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0008150	biological_process
cellular_component	GO:0005576	extracellular region
cellular_component	GO:0005575	cellular_component
molecular_function	GO:0003674	molecular_function

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Lsi03G009970.1	Lsi03G009970.1	mRNA

InterPro

Analysis Name: InterPro Annotations of Lagenaria siceraria
Date Performed: 2017-09-18


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR001283	Cysteine-rich secretory protein, allergen V5/Tpx-1-related	PRINTS	PR00837	V5TPXLIKE	coord: 118..134 score: 3.7E-18coord: 95..108 score: 3.7E-18coord: 48..66 score: 3.7E-18coord: 145..158 score: 3.7
IPR001283	Cysteine-rich secretory protein, allergen V5/Tpx-1-related	PANTHER	PTHR10334	CYSTEINE-RICH SECRETORY PROTEIN-RELATED	coord: 1..166 score: 1.7
IPR002413	Ves allergen	PRINTS	PR00838	V5ALLERGEN	coord: 93..108 score: 4.8E-8coord: 48..66 score: 4.8E-8coord: 117..136 score: 4.
IPR014044	CAP domain	GENE3D	G3DSA:3.40.33.10		coord: 26..164 score: 4.8
IPR014044	CAP domain	PFAM	PF00188	CAP	coord: 34..150 score: 2.0
IPR014044	CAP domain	SMART	SM00198	SCP_3	coord: 27..158 score: 5.7
IPR014044	CAP domain	unknown	SSF55797	PR-1-like	coord: 27..163 score: 2.62
IPR018244	Allergen V5/Tpx-1-related, conserved site	PROSITE	PS01010	CRISP_2	coord: 145..156 scor
None	No IPR available	PANTHER	PTHR10334:SF223	F2J10.6 PROTEIN-RELATED	coord: 1..166 score: 1.7

Synteny

The following gene(s) are orthologous to this gene:


Gene	Orthologue	Organism	Block
Lsi03G009970	CmoCh19G005460	Cucurbita moschata (Rifu)	cmolsiB467
Lsi03G009970	Cla006469	Watermelon (97103) v1	lsiwmB240
Lsi03G009970	ClCG10G009890	Watermelon (Charleston Gray)	lsiwcgB219
Lsi03G009970	MELO3C018539.2	Melon (DHL92) v3.6.1	lsimedB265
Lsi03G009970	Cla97C02G034120	Watermelon (97103) v2	lsiwmbB229
Lsi03G009970	Cla97C10G192910	Watermelon (97103) v2	lsiwmbB218
Lsi03G009970	Bhi11G000894	Wax gourd	lsiwgoB292
Lsi03G009970	CsaV3_7G007580	Cucumber (Chinese Long) v3	cuclsiB554

The following gene(s) are paralogous to this gene:

None

The following block(s) are covering this gene:


Gene	Organism	Block
Lsi03G009970	Wild cucumber (PI 183967)	cpilsiB282
Lsi03G009970	Wild cucumber (PI 183967)	cpilsiB534
Lsi03G009970	Cucumber (Chinese Long) v2	culsiB520
Lsi03G009970	Melon (DHL92) v3.5.1	lsimeB253
Lsi03G009970	Melon (DHL92) v3.5.1	lsimeB269
Lsi03G009970	Cucumber (Gy14) v2	cgyblsiB487
Lsi03G009970	Melon (DHL92) v3.6.1	lsimedB282
Lsi03G009970	Silver-seed gourd	carlsiB269
Lsi03G009970	Silver-seed gourd	carlsiB341
Lsi03G009970	Silver-seed gourd	carlsiB673
Lsi03G009970	Bottle gourd (USVL1VR-Ls)	lsilsiB126
Lsi03G009970	Cucumber (Gy14) v1	cgylsiB366
Lsi03G009970	Cucurbita maxima (Rimu)	cmalsiB115
Lsi03G009970	Cucurbita maxima (Rimu)	cmalsiB307
Lsi03G009970	Cucurbita maxima (Rimu)	cmalsiB483
Lsi03G009970	Cucurbita maxima (Rimu)	cmalsiB657
Lsi03G009970	Cucurbita moschata (Rifu)	cmolsiB108
Lsi03G009970	Cucurbita moschata (Rifu)	cmolsiB294
Lsi03G009970	Cucurbita moschata (Rifu)	cmolsiB646
Lsi03G009970	Cucurbita pepo (Zucchini)	cpelsiB168
Lsi03G009970	Cucurbita pepo (Zucchini)	cpelsiB207
Lsi03G009970	Cucurbita pepo (Zucchini)	cpelsiB340
Lsi03G009970	Cucurbita pepo (Zucchini)	cpelsiB548