Lsi03G007410 (gene) Bottle gourd (USVL1VR-Ls) | Cucurbit Genomics Database (CuGenDB)

Overview


Name	Lsi03G007410
Type	gene
Organism	Lagenaria siceraria (Bottle gourd (USVL1VR-Ls))
Description	Nitronate monooxygenase
Location	chr03 : 9991238 .. 9996831 (+)

Genome Viewer

Gene Expression

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: CDSthree_prime_UTR

Hold the cursor over a type above to highlight its positions in the sequence below.

TCCATTACGTCGTCGTTTCGCATTACTCGACAGATTATTCTGAGAGTGCGGCGTATTTGCTTACGTGTTGAATCCGGCAGCTCTCACGTAACCTTCAAAGCCGCCTCCCATGGCTGCCACTGCTACTTCCTTCCAATATATTCAAATTCACTCTTCTTACCATCAATCCATTATTGCCCACCGAAGACACAGAATCAGATGGCTTTTGGTGGAATACTTGGCTTCCAGTATGGCATAGTTCAGGCTCCGCTTGGACCTGATATCTCCTGTCCTGAGCTTGTTGCTGCCGTCGCCAATGCCGGCGGACTTGGTCTTCTCAGAGCCCCCGATTGGGTACTTTACTGTCTATTTCTCCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNCCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTCATTTAGCTGTGTTGGAAATGAATGTAATGTGTTGTGGAGAAGCATCTCTGATGTGCGATACGTGAGTGGAACCGGAGTTTCTCCTTTCGTAATGGATTTCAGTGATTGATTGTTCGATCATACGTGTTCGGATTTTGAAACTTTTAATTGTTGATCGGTTTGAATGATTGGCGTTTTCGGATTTTTGCAACTGGTTGTTGCATGGTTTTATAGGAATTCAAACGATTTGGTGATGATTGCTTATGAAATTATGGAGGAGTGTTGGGTTTAACTGGGGACTTAGTCGGGGGAATTGATAATGTAAATGAACAGTTGAATCTTTGATCTTGCCAATCTGTTGTGAACAGTTTATATAAGGTATGAATGTATAGAATTTTGTTAAAGTATTGATATAGTTAAACGTACCTTAACCTATGAGCTTAAGCTTTTCAGTAGATCGGTGATTTAACAGACTCATTTTCTCTTCCGTTAATGATGATTTCCATTTGTTGATATAATTAAAATTATAGTTTAGGATTGATGGGTGATTTAACAAATTTAATGCAAGCTTCTTATAGCTATTTTTTCCCTTCCTTTGTTCTTCTATTAGTCAAGAAACTAGTTATAAAATTTTAGTATCGGTTTCATAACTATTTTGTCTTTTGTTGTCTAAGAGCTAGTGTAGTGTAGTATTTAGCTTTAGTTTATACTTTCTACAACGGTTTTGAGATTTTAGCCAAAATTTGAAAAATCAATTAAATTTTTTTGAGAACTAAATGTTTTTGGTTTGTGAATCTTGCTATGATCTCAAAAACATTTATAAAATAAATGCATGTATTAAGGACTGAACAAACTTATCTTCTTAAATCGACAACATACAGTCAAATGATTATCAAACAGTCTTTTAATTATAAAGTCAAAAGGTGTATATCTTCCCATTTATCCTAACTATTTATCTACATCATTTCCTGTTCTTCACTCTATTTGAGTAGCATGGAGCTTGTACGCTTAAAATTTTTATGTGTACCAGGAGTCACCTGATTATGTACGAGAATTGATAAGGAAGACCCGAGCGTTAACAGATAAACCATTTGGAGTAGGTGTCATTTTAGCATTTCCTCATGAGCAAAATCTAAAGGCCATATTAGACGAGAAAGTAGCAGTTGTGCAAGTTTACTGGGGAGAATGTTCAAAGGATCTTGTAGATCAAGTTCATTCTGCTGGAGTGAAAATTATTCCACAAGTGAGTGTTCTCTTGCAAATCACATTAACAATGCATGTCCACAAGATCCAAATCAAGTCGATTGCTTGATCTTATCATTAAACTTTTTATGCACGGTTGTGGAGGCTTAATTTTCTAGAAAAATCAGTCATCTTATTAAATCACATGCTTCTCTTGTGTTGGTCCATCACTTCAGGCTTCATTATTCTGTTCTTGGATGATATTGGTCACTCATGTGGTATGTTACAATTTTAAAATCTACTTTTGTAGGTTGGGAGTGTTGAAGAAGCGAGTAAAGCAGTTGAAGTTGGTGTTGATGCAATAATTGTGCAAGGTCGGGAAGCGGGAGGGCATGTTATTGGTCAAGTATGGATCTTCTTCTTCCCAGAAAAATTAATTAATTCGAGATGCTATTGTATTTGTTTCTAGACTTCTTCCATTACAGTTTCTTTTTTCCATCTTCTAAAGGGGCTATGTTTTTGTACGGTTATTCTTCTTTGTAAAACGTTCTCTAAATTACTAGAAAAAGAAACGAATTAACAAGGAAAATAAGTTGACTTAGATAAGAAAGTTAGTTCATCAAACATCCCTTTCTCAATTCATTAAGGGACTGTGGTCTTAGTTCGGATACCTACATACTTACGCTGCTCCAAGTTCATCTTTTGAAGAGTATACAACATTGGTTTTGTTGAGCCAATGGTTATATTAGAAGCCTGTATTCAGTTTGAAATCAGCTTTAGACAGAACCGGATTTTTTAATATTGATCTTTATCATAAAAGTATTTAAACATTCTTCCTTTCTGACATCAGTCATGCATACATATTTTTAAGTAATTTCCTACTTTTTTCTTCTTTAAATTTCACTGGTAGTAAGAACTGCTAGTATTTTTGTTTGTTGCTTCCAGCCAGTGATATTACTTTGGTGTATGTTTTCATTAGTCATATTCTTCACCTCTCTTTACATCTGTATTTCAACTATATCGTAACTTTTCTCAAATATATAGTCTATAGAAGTTTCATTATCAGAAAAAATGAAAATAGTCTTTAGAAGTTTGACTTAGTTCTTTACAGGAAGGTTTGATTTCCATCTTACCAAGGATAGTTGATATTGTTGGGAATGAAGATATACCTGTAATTGCGGCTGGCGGTATTTCAGATTCCAGAGGTTATGTTGCTGCCCTTGCCCTTGGCGCTCATGGTGTCTGCCTTGGAACTAGGTTAGAAATGCTCAACTGTGATCATTCACAACTTAGAAAACTTATTTTAAAAAGCTGGTAGATCCATGCTACAAAATATTGCTCATTTAGAAACAATTTCCAGTATTAAGAATAGAATATTGCCACACGTTCGGTTCATTCAGGTTCTGTTCAGAGTCAACATATAAGTATGATAATAATGTCAGTGCAGAAATTTTATTAGTGGTCCGTGGCATATATTCTTAAACATATCTTATTTTCATTTATCCAAGGCATGAAAATATAGCCATGAGAATGGTGTGTGAGGAAGTTCTATGGAGGTTGATTGGTCATATCATAATCCATCCACTAAATAGTTCAGAGGCAGAGAACCTGTTCTTTTTGGTGTAACCCAAAGGTTGCCGGTTTTATTTATTTATTTTTGTTAGAGAGGCAAACAAACAATACCATCAAGAAATATCCAACTACTTCCTAACCTCATCAACACGAATGAGGGGGAGAATACTATCTAGAAAAACATCTTTCCAATCAAAGAACAATCTTGAGAGGAGGCAAAAGCTGTTATGGTATGGTCAGCTTTGTTGAGGAATATGACAAAAATGACAATATTAACAAGGTTCCCAACAAAAGGAATATCCTCAACGGGAACTCCAATTTTCGCAAGCGAGAGCTCCTTACACATCAGAAGATTAATCACTTCAAGTGCGCCTGATTCAACTTCAAGTGGCCTGATTCAACTTCAAGTGGAGGATTAGATGCCGTTATCCTTACTATATTATTGTAATTGGAACAATTATATAATCTAATAACGAAACATATCTCAGAAATTTTAGGTAATATTACTATTGATCTTTTGCATTGTCATAAGTATGAGTTAAATGGAATATTGCAGATTTGTTGCTACTGAAGAAAGCCGTGCTCACCCTACATACAAGAGAAAGTTGGTGGAACTGGAAGCAACTGATTATACAAACATTTTTGGCCGTGCAAGATGGCCCGGGGCACCACAGCGTGTTCTACAGACGCCCTTTTACGATGATTGGAAATCACTTCCTGCCAATGAAAATGAAAGCAACCAGCCTGTCATTGGCCGCTCAACAATTAATGGCTTGGTGAGCTCCCTCGTTATCTCTTTTCTACTTAAACAAGTGCATAAAAGGGAGTTATCTTCTTTTATTCTTCATGCTTGAAGAAATTCTGCAATTAGTTTATACGCTGGATCATTAAAAGATTAAATATTTAGGTTAAGGATATTCTGCTGTTCTGCTCTACTTTAAATCATCCTTCATTCAATGCATTCATGCTTGAAACAGGACATAGATATACGACGCTTTGCGGGTACCGTGCCAAATGCAACGACAAAAGGTGACATCGAGAGCATGGCAATGTATGCAGGGCAAGGAGTTGGACTAATCAAGGAAATTCTACCTGCAGGAGAAGTGGTAAGGAGGCTGGTGGAAGGTGCTCAACATCTTATTCAAACACATTTGAGTAGTTTTGTTCATAAAGAATGATGCTCAGAGCTGCATTATTTTTTGTTTTCTTGAAGAAATAAGATCAAAAATCCATATGTAGTTAGATGAATGGCTGAAAATTGGGCTCCACTGTTAATCATGAACTCAGCGAACCTCATGTTTAAATAAAGCAGTAATTGGTATTAGTTTATTTTTTAATCTTTATTTACAGGTTCGATTTTCAATCCCAAATTTATCCATTTTCAGGAAGCATTTCAACGAATTTAG

mRNA sequence

TCCATTACGTCGTCGTTTCGCATTACTCGACAGATTATTCTGAGAGTGCGGCGTATTTGCTTACGTGTTGAATCCGGCAGCTCTCACGTAACCTTCAAAGCCGCCTCCCATGGCTGCCACTGCTACTTCCTTCCAATATATTCAAATTCACTCTTCTTACCATCAATCCATTATTGCCCACCGAAGACACAGAATCAGATGGCTTTTGGTGGAATACTTGGCTTCCAGTATGGCATAGTTCAGGCTCCGCTTGGACCTGATATCTCCTGTCCTGAGCTTGTTGCTGCCGTCGCCAATGCCGGCGGACTTGGTCTTCTCAGAGCCCCCGATTGGGAGTCACCTGATTATGTACGAGAATTGATAAGGAAGACCCGAGCGTTAACAGATAAACCATTTGGAGTAGGTGTCATTTTAGCATTTCCTCATGAGCAAAATCTAAAGGCCATATTAGACGAGAAAGTAGCAGTTGTGCAAGTTTACTGGGGAGAATGTTCAAAGGATCTTGTAGATCAAGTTCATTCTGCTGGAGTGAAAATTATTCCACAAGTTGGGAGTGTTGAAGAAGCGAGTAAAGCAGTTGAAGTTGGTGTTGATGCAATAATTGTGCAAGGTCGGGAAGCGGGAGGGCATGTTATTGGTCAAGAAGGTTTGATTTCCATCTTACCAAGGATAGTTGATATTGTTGGGAATGAAGATATACCTGTAATTGCGGCTGGCGGTATTTCAGATTCCAGAGGTTATGTTGCTGCCCTTGCCCTTGGCGCTCATGGTGTCTGCCTTGGAACTAGGTTAGAAATGCTCAACTGTGATCATTCACAACTTAGAAAACTTATTTTAAAAAGCTGGTTCTGTTCAGAGTCAACATATAAATTTGTTGCTACTGAAGAAAGCCGTGCTCACCCTACATACAAGAGAAAGTTGGTGGAACTGGAAGCAACTGATTATACAAACATTTTTGGCCGTGCAAGATGGCCCGGGGCACCACAGCGTGTTCTACAGACGCCCTTTTACGATGATTGGAAATCACTTCCTGCCAATGAAAATGAAAGCAACCAGCCTGTCATTGGCCGCTCAACAATTAATGGCTTGGACATAGATATACGACGCTTTGCGGGTACCGTGCCAAATGCAACGACAAAAGGTGACATCGAGAGCATGGCAATGTATGCAGGGCAAGGAGTTGGACTAATCAAGGAAATTCTACCTGCAGGAGAAGTGGTAAGGAGGCTGGTGGAAGGTGCTCAACATCTTATTCAAACACATTTGAGTAGTTTTGTTCATAAAGAATGATGCTCAGAGCTGCATTATTTTTTGTTTTCTTGAAGAAATAAGATCAAAAATCCATATGTAGTTAGATGAATGGCTGAAAATTGGGCTCCACTGTTAATCATGAACTCAGCGAACCTCATGTTTAAATAAAGCAGTAATTGGTATTAGTTTATTTTTTAATCTTTATTTACAGGTTCGATTTTCAATCCCAAATTTATCCATTTTCAGGAAGCATTTCAACGAATTTAG

Coding sequence (CDS)

TCCATTACGTCGTCGTTTCGCATTACTCGACAGATTATTCTGAGAGTGCGGCGTATTTGCTTACGTGTTGAATCCGGCAGCTCTCACGTAACCTTCAAAGCCGCCTCCCATGGCTGCCACTGCTACTTCCTTCCAATATATTCAAATTCACTCTTCTTACCATCAATCCATTATTGCCCACCGAAGACACAGAATCAGATGGCTTTTGGTGGAATACTTGGCTTCCAGTATGGCATAGTTCAGGCTCCGCTTGGACCTGATATCTCCTGTCCTGAGCTTGTTGCTGCCGTCGCCAATGCCGGCGGACTTGGTCTTCTCAGAGCCCCCGATTGGGAGTCACCTGATTATGTACGAGAATTGATAAGGAAGACCCGAGCGTTAACAGATAAACCATTTGGAGTAGGTGTCATTTTAGCATTTCCTCATGAGCAAAATCTAAAGGCCATATTAGACGAGAAAGTAGCAGTTGTGCAAGTTTACTGGGGAGAATGTTCAAAGGATCTTGTAGATCAAGTTCATTCTGCTGGAGTGAAAATTATTCCACAAGTTGGGAGTGTTGAAGAAGCGAGTAAAGCAGTTGAAGTTGGTGTTGATGCAATAATTGTGCAAGGTCGGGAAGCGGGAGGGCATGTTATTGGTCAAGAAGGTTTGATTTCCATCTTACCAAGGATAGTTGATATTGTTGGGAATGAAGATATACCTGTAATTGCGGCTGGCGGTATTTCAGATTCCAGAGGTTATGTTGCTGCCCTTGCCCTTGGCGCTCATGGTGTCTGCCTTGGAACTAGGTTAGAAATGCTCAACTGTGATCATTCACAACTTAGAAAACTTATTTTAAAAAGCTGGTTCTGTTCAGAGTCAACATATAAATTTGTTGCTACTGAAGAAAGCCGTGCTCACCCTACATACAAGAGAAAGTTGGTGGAACTGGAAGCAACTGATTATACAAACATTTTTGGCCGTGCAAGATGGCCCGGGGCACCACAGCGTGTTCTACAGACGCCCTTTTACGATGATTGGAAATCACTTCCTGCCAATGAAAATGAAAGCAACCAGCCTGTCATTGGCCGCTCAACAATTAATGGCTTGGACATAGATATACGACGCTTTGCGGGTACCGTGCCAAATGCAACGACAAAAGGTGACATCGAGAGCATGGCAATGTATGCAGGGCAAGGAGTTGGACTAATCAAGGAAATTCTACCTGCAGGAGAAGTGGTAAGGAGGCTGGTGGAAGGTGCTCAACATCTTATTCAAACACATTTGAGTAGTTTTGTTCATAAAGAATGA

Protein sequence

SITSSFRITRQIILRVRRICLRVESGSSHVTFKAASHGCHCYFLPIYSNSLFLPSIHYCPPKTQNQMAFGGILGFQYGIVQAPLGPDISCPELVAAVANAGGLGLLRAPDWESPDYVRELIRKTRALTDKPFGVGVILAFPHEQNLKAILDEKVAVVQVYWGECSKDLVDQVHSAGVKIIPQVGSVEEASKAVEVGVDAIIVQGREAGGHVIGQEGLISILPRIVDIVGNEDIPVIAAGGISDSRGYVAALALGAHGVCLGTRLEMLNCDHSQLRKLILKSWFCSESTYKFVATEESRAHPTYKRKLVELEATDYTNIFGRARWPGAPQRVLQTPFYDDWKSLPANENESNQPVIGRSTINGLDIDIRRFAGTVPNATTKGDIESMAMYAGQGVGLIKEILPAGEVVRRLVEGAQHLIQTHLSSFVHKE

Homology

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BLAST of Lsi03G007410 vs. Swiss-Prot
Match: 2NPD_NEUCR (Nitronate monooxygenase OS=Neurospora crassa (strain ATCC 24698 / 74-OR23-1A / CBS 708.71 / DSM 1257 / FGSC 987) GN=ncd-2 PE=1 SV=1)

HSP 1 Score: 110.5 bits (275), Expect = 4.5e-23
Identity = 107/391 (27.37%), Postives = 166/391 (42.46%), Query Frame = 1


Query: 79  IVQAPLGPDISCPELVAAVANAGGLGLLRAPDWESP--DYVREL------IRKTRALTDK 138
           I+ AP+   I+   L A V+ AGG+G +       P   ++  L       R    LTD+
Sbjct: 33  IISAPMYL-IANGTLAAEVSKAGGIGFVAGGSDFRPGSSHLTALSTELASARSRLGLTDR 92

Query: 139 PF----GVGVILAFPHEQNLKAILDEKVAVV-----QVYW----------------GECS 198
           P     G+GV L   H  ++  + D  + ++     Q  W                   +
Sbjct: 93  PLTPLPGIGVGLILTHTISVPYVTDTVLPILIEHSPQAVWLFANDPDFEASSEPGAKGTA 152

Query: 199 KDLVDQVHSAGVKIIPQVGSVEEASKAVEVGVDAIIVQGREAGGHVIGQ-EGLISILPRI 258
           K +++ +H++G  +  QVG+V++A KA   G D I+ QG +AGGH +    G++S++P +
Sbjct: 153 KQIIEALHASGFVVFFQVGTVKDARKAAADGADVIVAQGIDAGGHQLATGSGIVSLVPEV 212

Query: 259 VDIVGNE----DIPVIAAGGISDSRGYVAALALGAHGVCLGTRLEMLNCDHSQLRKLILK 318
            D++  E    ++ V+AAGG++D RG V AL LGA GV LGTR                 
Sbjct: 213 RDMLDREFKEREVVVVAAGGVADGRGVVGALGLGAEGVVLGTRFT--------------- 272

Query: 319 SWFCSESTYKFVATEESRAHPTYKRKLVELEATD----------YTNIFGRARWPGA-PQ 378
                      VA E S   P ++RK++ LE  D          +  I     W      
Sbjct: 273 -----------VAVEAST--PEFRRKVI-LETNDGGLNTVKSHFHDQINCNTIWHNVYDG 332

Query: 379 RVLQTPFYDDWKS-LPANENESNQPVIGRSTINGLDIDIRRFAGTVPNATTKGDIESMAM 420
           R ++   YDD  + +P  EN                            A + GD      
Sbjct: 333 RAVRNASYDDHAAGVPFEENHKK----------------------FKEAASSGDNSRAVT 371

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BLAST of Lsi03G007410 vs. Swiss-Prot
Match: 2NPD_STAA3 (Probable nitronate monooxygenase OS=Staphylococcus aureus (strain USA300) GN=SAUSA300_0825 PE=3 SV=1)

HSP 1 Score: 106.7 bits (265), Expect = 6.5e-22
Identity = 79/236 (33.47%), Postives = 119/236 (50.42%), Query Frame = 1


Query: 72  ILGFQYGIVQAPLGPDISCPELVAAVANAGGLGLLRAPDWESPDYVRELIRKTRALTDKP 131
           +L  +Y I+QA +    + P+LVA+V+N+GGLG + A  + +   + + I   R LT   
Sbjct: 10  MLSIEYPIIQAGMAGSTT-PKLVASVSNSGGLGTIGA-GYFNTQQLEDEIDYVRQLTSNS 69

Query: 132 FGVGV--------------------------------ILAFPHEQNLKAILD----EKVA 191
           FGV V                                ++    EQ  K  +D    ++V 
Sbjct: 70  FGVNVFVPSQQSYTSSQIENMNAWLKPYRRALHLEEPVVKITEEQQFKCHIDTIIKKQVP 129

Query: 192 VVQVYWGECSKDLVDQVHSAGVKIIPQVGSVEEASKAVEVGVDAIIVQGREAGGH----- 251
           V    +G  S+ ++ ++ +A VK+I    SV+EA    + G+DAI+ QG EAGGH     
Sbjct: 130 VCCFTFGIPSEQIISRLKAANVKLIGTATSVDEAIANEKAGMDAIVAQGSEAGGHRGSFL 189

Query: 252 ----VIGQEGLISILPRIVDIVGNEDIPVIAAGGISDSRGYVAALALGAHGVCLGT 263
                +   G IS++P+IVD+V    IPVIAAGGI D RG +A++ LGA GV +GT
Sbjct: 190 KPKNQLPMVGTISLVPQIVDVV---SIPVIAAGGIMDGRGVLASIVLGAEGVQMGT 240

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BLAST of Lsi03G007410 vs. Swiss-Prot
Match: 2NPD_STAA8 (Probable nitronate monooxygenase OS=Staphylococcus aureus (strain NCTC 8325) GN=SAOUHSC_00855 PE=3 SV=1)

HSP 1 Score: 106.7 bits (265), Expect = 6.5e-22
Identity = 79/236 (33.47%), Postives = 119/236 (50.42%), Query Frame = 1


Query: 72  ILGFQYGIVQAPLGPDISCPELVAAVANAGGLGLLRAPDWESPDYVRELIRKTRALTDKP 131
           +L  +Y I+QA +    + P+LVA+V+N+GGLG + A  + +   + + I   R LT   
Sbjct: 10  MLSIEYPIIQAGMAGSTT-PKLVASVSNSGGLGTIGA-GYFNTQQLEDEIDYVRQLTSNS 69

Query: 132 FGVGV--------------------------------ILAFPHEQNLKAILD----EKVA 191
           FGV V                                ++    EQ  K  +D    ++V 
Sbjct: 70  FGVNVFVPSQQSYTSSQIENMNAWLKPYRRALHLEEPVVKITEEQQFKCHIDTIIKKQVP 129

Query: 192 VVQVYWGECSKDLVDQVHSAGVKIIPQVGSVEEASKAVEVGVDAIIVQGREAGGH----- 251
           V    +G  S+ ++ ++ +A VK+I    SV+EA    + G+DAI+ QG EAGGH     
Sbjct: 130 VCCFTFGIPSEQIISRLKAANVKLIGTATSVDEAIANEKAGMDAIVAQGSEAGGHRGSFL 189

Query: 252 ----VIGQEGLISILPRIVDIVGNEDIPVIAAGGISDSRGYVAALALGAHGVCLGT 263
                +   G IS++P+IVD+V    IPVIAAGGI D RG +A++ LGA GV +GT
Sbjct: 190 KPKNQLPMVGTISLVPQIVDVV---SIPVIAAGGIMDGRGVLASIVLGAEGVQMGT 240

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BLAST of Lsi03G007410 vs. Swiss-Prot
Match: 2NPD_STAAC (Probable nitronate monooxygenase OS=Staphylococcus aureus (strain COL) GN=SACOL0922 PE=3 SV=1)

HSP 1 Score: 106.7 bits (265), Expect = 6.5e-22
Identity = 79/236 (33.47%), Postives = 119/236 (50.42%), Query Frame = 1


Query: 72  ILGFQYGIVQAPLGPDISCPELVAAVANAGGLGLLRAPDWESPDYVRELIRKTRALTDKP 131
           +L  +Y I+QA +    + P+LVA+V+N+GGLG + A  + +   + + I   R LT   
Sbjct: 10  MLSIEYPIIQAGMAGSTT-PKLVASVSNSGGLGTIGA-GYFNTQQLEDEIDYVRQLTSNS 69

Query: 132 FGVGV--------------------------------ILAFPHEQNLKAILD----EKVA 191
           FGV V                                ++    EQ  K  +D    ++V 
Sbjct: 70  FGVNVFVPSQQSYTSSQIENMNAWLKPYRRALHLEEPVVKITEEQQFKCHIDTIIKKQVP 129

Query: 192 VVQVYWGECSKDLVDQVHSAGVKIIPQVGSVEEASKAVEVGVDAIIVQGREAGGH----- 251
           V    +G  S+ ++ ++ +A VK+I    SV+EA    + G+DAI+ QG EAGGH     
Sbjct: 130 VCCFTFGIPSEQIISRLKAANVKLIGTATSVDEAIANEKAGMDAIVAQGSEAGGHRGSFL 189

Query: 252 ----VIGQEGLISILPRIVDIVGNEDIPVIAAGGISDSRGYVAALALGAHGVCLGT 263
                +   G IS++P+IVD+V    IPVIAAGGI D RG +A++ LGA GV +GT
Sbjct: 190 KPKNQLPMVGTISLVPQIVDVV---SIPVIAAGGIMDGRGVLASIVLGAEGVQMGT 240

[Close]

BLAST of Lsi03G007410 vs. Swiss-Prot
Match: 2NPD_STAAE (Probable nitronate monooxygenase OS=Staphylococcus aureus (strain Newman) GN=NWMN_0792 PE=3 SV=1)

HSP 1 Score: 106.7 bits (265), Expect = 6.5e-22
Identity = 79/236 (33.47%), Postives = 119/236 (50.42%), Query Frame = 1


Query: 72  ILGFQYGIVQAPLGPDISCPELVAAVANAGGLGLLRAPDWESPDYVRELIRKTRALTDKP 131
           +L  +Y I+QA +    + P+LVA+V+N+GGLG + A  + +   + + I   R LT   
Sbjct: 10  MLSIEYPIIQAGMAGSTT-PKLVASVSNSGGLGTIGA-GYFNTQQLEDEIDYVRQLTSNS 69

Query: 132 FGVGV--------------------------------ILAFPHEQNLKAILD----EKVA 191
           FGV V                                ++    EQ  K  +D    ++V 
Sbjct: 70  FGVNVFVPSQQSYTSSQIENMNAWLKPYRRALHLEEPVVKITEEQQFKCHIDTIIKKQVP 129

Query: 192 VVQVYWGECSKDLVDQVHSAGVKIIPQVGSVEEASKAVEVGVDAIIVQGREAGGH----- 251
           V    +G  S+ ++ ++ +A VK+I    SV+EA    + G+DAI+ QG EAGGH     
Sbjct: 130 VCCFTFGIPSEQIISRLKAANVKLIGTATSVDEAIANEKAGMDAIVAQGSEAGGHRGSFL 189

Query: 252 ----VIGQEGLISILPRIVDIVGNEDIPVIAAGGISDSRGYVAALALGAHGVCLGT 263
                +   G IS++P+IVD+V    IPVIAAGGI D RG +A++ LGA GV +GT
Sbjct: 190 KPKNQLPMVGTISLVPQIVDVV---SIPVIAAGGIMDGRGVLASIVLGAEGVQMGT 240

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BLAST of Lsi03G007410 vs. TrEMBL
Match: A0A0A0K422_CUCSA (Uncharacterized protein OS=Cucumis sativus GN=Csa_7G064010 PE=4 SV=1)

HSP 1 Score: 705.3 bits (1819), Expect = 4.6e-200
Identity = 364/418 (87.08%), Postives = 375/418 (89.71%), Query Frame = 1


Query: 16  VRRICLRVESGSSHVTFKAASHGCHCYF--LPIYSNSLF--LPSIHYCPPKTQNQMAFGG 75
           +RR CLRVESGSSHV FKAASHG HCYF  LPIYSNSL   LP+ HYCP KTQ QM FGG
Sbjct: 1   MRRNCLRVESGSSHVIFKAASHGFHCYFPSLPIYSNSLLFSLPT-HYCPLKTQIQMGFGG 60

Query: 76  ILGFQYGIVQAPLGPDISCPELVAAVANAGGLGLLRAPDWESPDYVRELIRKTRALTDKP 135
           +LGFQYGIVQAPLGPDIS PELVAAVANAGGLGLLRAPDWESPDYVRELIRKTRALTDKP
Sbjct: 61  LLGFQYGIVQAPLGPDISGPELVAAVANAGGLGLLRAPDWESPDYVRELIRKTRALTDKP 120

Query: 136 FGVGVILAFPHEQNLKAILDEKVAVVQVYWGECSKDLVDQVHSAGVKIIPQVGSVEEASK 195
           FG+GVILAFPHEQNLKAILDEKVAVVQVYWGECSKDLVDQVHSAGVKIIPQVGSVEEASK
Sbjct: 121 FGIGVILAFPHEQNLKAILDEKVAVVQVYWGECSKDLVDQVHSAGVKIIPQVGSVEEASK 180

Query: 196 AVEVGVDAIIVQGREAGGHVIGQEGLISILPRIVDIVGNEDIPVIAAGGISDSRGYVAAL 255
           AV+VGVDAIIVQGREAGGHVIGQEGLISILPR+V+IVG++DIPVIAAG ISDSRGYVAAL
Sbjct: 181 AVDVGVDAIIVQGREAGGHVIGQEGLISILPRVVEIVGDKDIPVIAAGSISDSRGYVAAL 240

Query: 256 ALGAHGVCLGTRLEMLNCDHSQLRKLILKSWFCSESTYKFVATEESRAHPTYKRKLVELE 315
           ALGA GVCLGTR                           FVATEES AHPTYKRKLVELE
Sbjct: 241 ALGARGVCLGTR---------------------------FVATEESNAHPTYKRKLVELE 300

Query: 316 ATDYTNIFGRARWPGAPQRVLQTPFYDDWKSLPANENESNQPVIGRSTINGLDIDIRRFA 375
           ATDYTNIFGRARWPGAPQRVLQTPFYDDWKSLPANENESNQPVIGRSTINGLDIDIRRFA
Sbjct: 301 ATDYTNIFGRARWPGAPQRVLQTPFYDDWKSLPANENESNQPVIGRSTINGLDIDIRRFA 360

Query: 376 GTVPNATTKGDIESMAMYAGQGVGLIKEILPAGEVVRRLVEGAQHLIQTHLSSFVHKE 430
           GTVPNATTKGDIESMAMYAGQGVGLIKEILPAGEVVRRLVEGAQHLI+THLSSFVHK+
Sbjct: 361 GTVPNATTKGDIESMAMYAGQGVGLIKEILPAGEVVRRLVEGAQHLIRTHLSSFVHKD 390

[Close]

BLAST of Lsi03G007410 vs. TrEMBL
Match: B9IJE3_POPTR (Uncharacterized protein OS=Populus trichocarpa GN=POPTR_0017s07870g PE=4 SV=1)

HSP 1 Score: 531.2 bits (1367), Expect = 1.2e-147
Identity = 266/353 (75.35%), Postives = 296/353 (83.85%), Query Frame = 1


Query: 67  MAFGGILGFQYGIVQAPLGPDISCPELVAAVANAGGLGLLRAPDWESPDYVRELIRKTRA 126
           M + GILGF+YGIVQAPLGPDIS PELVAAVANAGGLGLLRAPDWESPDY+RELIRKTR+
Sbjct: 1   MGWRGILGFEYGIVQAPLGPDISGPELVAAVANAGGLGLLRAPDWESPDYLRELIRKTRS 60

Query: 127 LTDKPFGVGVILAFPHEQNLKAILDEKVAVVQVYWGECSKDLVDQVHSAGVKIIPQVGSV 186
           LTDKPFGVG++LAFPHE+NLK ILDEKVAV+QVYWGECSK+LV + H AGVK++PQVGS 
Sbjct: 61  LTDKPFGVGLVLAFPHEENLKVILDEKVAVLQVYWGECSKELVLEAHLAGVKVVPQVGSF 120

Query: 187 EEASKAVEVGVDAIIVQGREAGGHVIGQEGLISILPRIVDIVGNEDIPVIAAGGISDSRG 246
           E+A KA++ GVD IIVQGREAGGHVIGQEGLISILPR+VD+VG+ DIPVIAAGGI ++RG
Sbjct: 121 EDARKAIDAGVDGIIVQGREAGGHVIGQEGLISILPRVVDLVGDRDIPVIAAGGIVNARG 180

Query: 247 YVAALALGAHGVCLGTRLEMLNCDHSQLRKLILKSWFCSESTYKFVATEESRAHPTYKRK 306
           YVAALALGA GVCLGTR                           FVATEES AHPTYKRK
Sbjct: 181 YVAALALGAKGVCLGTR---------------------------FVATEESNAHPTYKRK 240

Query: 307 LVELEATDYTNIFGRARWPGAPQRVLQTPFYDDWKSLPANENESNQPVIGRSTINGLDID 366
           LVEL+ T+YTNIFGRARWP APQR L TPF++DWKSLP +ENE NQPVIGRSTING + +
Sbjct: 241 LVELDRTEYTNIFGRARWPNAPQRALVTPFFNDWKSLPPHENEINQPVIGRSTINGEEKE 300

Query: 367 IRRFAGTVPNATTKGDIESMAMYAGQGVGLIKEILPAGEVVRRLVEGAQHLIQ 420
           IRRF G VPN TT GDIESMAMYAGQGVGLIKEI PA EV++RLVEGAQ LI+
Sbjct: 301 IRRFGGPVPNMTTTGDIESMAMYAGQGVGLIKEIWPASEVIKRLVEGAQQLIR 326

[Close]

BLAST of Lsi03G007410 vs. TrEMBL
Match: A0A067L9X1_JATCU (Uncharacterized protein OS=Jatropha curcas GN=JCGZ_24913 PE=4 SV=1)

HSP 1 Score: 522.3 bits (1344), Expect = 5.5e-145
Identity = 263/358 (73.46%), Postives = 294/358 (82.12%), Query Frame = 1


Query: 67  MAFGGILGFQYGIVQAPLGPDISCPELVAAVANAGGLGLLRAPDWESPDYVRELIRKTRA 126
           M + GILGF+YGIVQAPLGPDIS PELVAAVANAGGLG LRAPDWESPDYVR LIRKTR 
Sbjct: 1   MVWRGILGFEYGIVQAPLGPDISGPELVAAVANAGGLGFLRAPDWESPDYVRGLIRKTRT 60

Query: 127 LTDKPFGVGVILAFPHEQNLKAILDEKVAVVQVYWGECSKDLVDQVHSAGVKIIPQVGSV 186
           LTDKPFG+GV+LAFPHE+N+KAILDEKVAV+QVYWG+CS++LV + H AGVK++PQVGS 
Sbjct: 61  LTDKPFGIGVVLAFPHEENVKAILDEKVAVLQVYWGDCSEELVLKAHQAGVKVVPQVGSY 120

Query: 187 EEASKAVEVGVDAIIVQGREAGGHVIGQEGLISILPRIVDIVGNEDIPVIAAGGISDSRG 246
           E A KA+ VGVDAIIVQGREAGGHVIGQEGLIS+LPR+VD+VG   IPVIAAGGI D+RG
Sbjct: 121 EGAEKAISVGVDAIIVQGREAGGHVIGQEGLISLLPRVVDLVGVRGIPVIAAGGIVDARG 180

Query: 247 YVAALALGAHGVCLGTRLEMLNCDHSQLRKLILKSWFCSESTYKFVATEESRAHPTYKRK 306
           YVAALALGA GVCLGTR                           F+A EES AHP YKRK
Sbjct: 181 YVAALALGAQGVCLGTR---------------------------FLAAEESYAHPIYKRK 240

Query: 307 LVELEATDYTNIFGRARWPGAPQRVLQTPFYDDWKSLPANENESNQPVIGRSTINGLDID 366
           L+EL  T++TN+FGRARWP APQRVL TPF++DWKSLP++E+E NQPVIGRSTING + +
Sbjct: 241 LIELSKTEHTNVFGRARWPNAPQRVLVTPFFNDWKSLPSHESEINQPVIGRSTINGEEKE 300

Query: 367 IRRFAGTVPNATTKGDIESMAMYAGQGVGLIKEILPAGEVVRRLVEGAQHLIQTHLSS 425
           IRRF GTVPN TT GDIESMAMYAGQGVGLI EILPA EVV+ LVEGAQ LIQ  LSS
Sbjct: 301 IRRFGGTVPNRTTTGDIESMAMYAGQGVGLIDEILPASEVVKMLVEGAQQLIQQELSS 331

[Close]

BLAST of Lsi03G007410 vs. TrEMBL
Match: A0A061E2B3_THECC (Aldolase-type TIM barrel family protein isoform 1 OS=Theobroma cacao GN=TCM_007438 PE=4 SV=1)

HSP 1 Score: 520.8 bits (1340), Expect = 1.6e-144
Identity = 257/357 (71.99%), Postives = 300/357 (84.03%), Query Frame = 1


Query: 67  MAFGGILGFQYGIVQAPLGPDISCPELVAAVANAGGLGLLRAPDWESPDYVRELIRKTRA 126
           M + GILGF+YGIVQAPLGPDI+ PELVAAVANAGGLGLLRAPDWESP+Y++ELIRKTR 
Sbjct: 1   MGWRGILGFEYGIVQAPLGPDIAGPELVAAVANAGGLGLLRAPDWESPEYLKELIRKTRM 60

Query: 127 LTDKPFGVGVILAFPHEQNLKAILDEKVAVVQVYWGECSKDLVDQVHSAGVKIIPQVGSV 186
           LTDKPFGVGV+LAFPH++N+KAIL+EKVAV+Q+YWGECSK+LV + H+AGVK++PQVGSV
Sbjct: 61  LTDKPFGVGVVLAFPHKENIKAILEEKVAVLQLYWGECSKELVLEAHNAGVKVVPQVGSV 120

Query: 187 EEASKAVEVGVDAIIVQGREAGGHVIGQEGLISILPRIVDIVGNEDIPVIAAGGISDSRG 246
           EEA KA+ VGVDAIIVQGREAGGHVIGQEGLIS+LP++VD+VG+ DIPVIAAG I D+RG
Sbjct: 121 EEAKKALNVGVDAIIVQGREAGGHVIGQEGLISLLPQVVDLVGDRDIPVIAAGSIVDARG 180

Query: 247 YVAALALGAHGVCLGTRLEMLNCDHSQLRKLILKSWFCSESTYKFVATEESRAHPTYKRK 306
           YVAAL LGA G+C+GTR                           F+AT ES AHPTYKRK
Sbjct: 181 YVAALTLGAKGICMGTR---------------------------FLATHESYAHPTYKRK 240

Query: 307 LVELEATDYTNIFGRARWPGAPQRVLQTPFYDDWKSLPANENESNQPVIGRSTINGLDID 366
           L+E + T+YT++FGRARWPGAP RVLQTPF+ DWKSLP +ENE+ QP+IGRS I+G++ +
Sbjct: 241 LIEHDKTEYTDVFGRARWPGAPHRVLQTPFFCDWKSLPVHENETTQPIIGRSIIHGVEKE 300

Query: 367 IRRFAGTVPNATTKGDIESMAMYAGQGVGLIKEILPAGEVVRRLVEGAQHLIQTHLS 424
           IRRFAGTVPN TT GDIESMAMYAGQ VGLIKEI+PAGEVV+RLVEGAQ  IQ  LS
Sbjct: 301 IRRFAGTVPNPTTTGDIESMAMYAGQSVGLIKEIVPAGEVVKRLVEGAQLAIQQILS 330

[Close]

BLAST of Lsi03G007410 vs. TrEMBL
Match: W9SAP4_9ROSA (Putative nitronate monooxygenase OS=Morus notabilis GN=L484_010808 PE=4 SV=1)

HSP 1 Score: 520.4 bits (1339), Expect = 2.1e-144
Identity = 257/357 (71.99%), Postives = 298/357 (83.47%), Query Frame = 1


Query: 67  MAFGGILGFQYGIVQAPLGPDISCPELVAAVANAGGLGLLRAPDWESPDYVRELIRKTRA 126
           M   GILGF+YGIVQAPLGPDIS PELVAAVANAGGLGLLRAPDWESPDY++ELIR+TR+
Sbjct: 1   MGLRGILGFEYGIVQAPLGPDISGPELVAAVANAGGLGLLRAPDWESPDYLKELIRRTRS 60

Query: 127 LTDKPFGVGVILAFPHEQNLKAILDEKVAVVQVYWGECSKDLVDQVHSAGVKIIPQVGSV 186
           LTDKPFGVGV+LAFPHE+N+KAIL+EKVAV+QVYWG+CS+DLV + H AGVK++PQVGS+
Sbjct: 61  LTDKPFGVGVVLAFPHEENVKAILEEKVAVLQVYWGDCSQDLVVEAHRAGVKVVPQVGSI 120

Query: 187 EEASKAVEVGVDAIIVQGREAGGHVIGQEGLISILPRIVDIVGNEDIPVIAAGGISDSRG 246
           EEA KA+E GVDA+IVQGREAGGHVI +E L  +LPR+VD+VG++DIPVIAAGGI D+RG
Sbjct: 121 EEAKKAIEAGVDAVIVQGREAGGHVISKEALFPLLPRVVDLVGDQDIPVIAAGGIVDARG 180

Query: 247 YVAALALGAHGVCLGTRLEMLNCDHSQLRKLILKSWFCSESTYKFVATEESRAHPTYKRK 306
           YVAALALGA GVCLGTR                           F+ATEES AHP YK+K
Sbjct: 181 YVAALALGAKGVCLGTR---------------------------FLATEESYAHPIYKKK 240

Query: 307 LVELEATDYTNIFGRARWPGAPQRVLQTPFYDDWKSLPANENESNQPVIGRSTINGLDID 366
           L+E E T+YT+IFGRARWP AP RVLQTPF+++WKSL A+ENE+NQPVIG+STI+G + +
Sbjct: 241 LIEFERTEYTDIFGRARWPNAPHRVLQTPFFNEWKSLSAHENETNQPVIGQSTIHGKEKE 300

Query: 367 IRRFAGTVPNATTKGDIESMAMYAGQGVGLIKEILPAGEVVRRLVEGAQHLIQTHLS 424
           I RFAGTVPN TT GDIESMAMYAG+GVGLIKEILPAGEVV+RLVEGA  LIQ   S
Sbjct: 301 IHRFAGTVPNVTTTGDIESMAMYAGEGVGLIKEILPAGEVVKRLVEGALVLIQQKFS 330

[Close]

BLAST of Lsi03G007410 vs. TAIR10
Match: AT5G64250.2 (AT5G64250.2 Aldolase-type TIM barrel family protein)

HSP 1 Score: 514.2 bits (1323), Expect = 7.6e-146
Identity = 252/348 (72.41%), Postives = 291/348 (83.62%), Query Frame = 1


Query: 71  GILGFQYGIVQAPLGPDISCPELVAAVANAGGLGLLRAPDWESPDYVRELIRKTRALTDK 130
           GILGF+YGIVQAPLGPDIS PELVAAVANAGG+GLLR PDWE PDY+RELIRKT+ LT+K
Sbjct: 3   GILGFEYGIVQAPLGPDISGPELVAAVANAGGIGLLRCPDWECPDYLRELIRKTKTLTEK 62

Query: 131 PFGVGVILAFPHEQNLKAILDEKVAVVQVYWGECSKDLVDQVHSAGVKIIPQVGSVEEAS 190
           PFG+GV+LAFPH+ N+K IL+EKVAV+Q+YWG+CS++LVD  H AGVK+IPQVG+VEEA 
Sbjct: 63  PFGIGVVLAFPHDLNIKTILEEKVAVLQLYWGDCSQELVDDAHFAGVKVIPQVGNVEEAR 122

Query: 191 KAVEVGVDAIIVQGREAGGHVIGQEGLISILPRIVDIVGNEDIPVIAAGGISDSRGYVAA 250
           KAV VGVDAIIVQGREAGGHVIG++GL S+LPR+VD+VG  DIPVIAAGGI D RGYVAA
Sbjct: 123 KAVAVGVDAIIVQGREAGGHVIGKDGLFSLLPRVVDLVGERDIPVIAAGGIVDGRGYVAA 182

Query: 251 LALGAHGVCLGTRLEMLNCDHSQLRKLILKSWFCSESTYKFVATEESRAHPTYKRKLVEL 310
           L+LGA GVCLGTR                           FVAT ES AHP YKRKL+E 
Sbjct: 183 LSLGAQGVCLGTR---------------------------FVATHESYAHPIYKRKLIEY 242

Query: 311 EATDYTNIFGRARWPGAPQRVLQTPFYDDWKSLPANENESNQPVIGRSTINGLDIDIRRF 370
           E T+YT++FGRARWPGAPQRVL+TPF+DDW+SLPA+ENE NQP+IGRSTI+G++ +IRRF
Sbjct: 243 EKTEYTDVFGRARWPGAPQRVLETPFFDDWRSLPAHENEVNQPIIGRSTIHGVEKEIRRF 302

Query: 371 AGTVPNATTKGDIESMAMYAGQGVGLIKEILPAGEVVRRLVEGAQHLI 419
           +GTVPN TT GD+ESMAMYAGQ VGLIKEILPAGEVV+ LVE AQ LI
Sbjct: 303 SGTVPNMTTTGDLESMAMYAGQSVGLIKEILPAGEVVKSLVEEAQALI 323

[Close]

BLAST of Lsi03G007410 vs. NCBI nr
Match: gi|778724449|ref|XP_011658807.1| (PREDICTED: nitronate monooxygenase isoform X1 [Cucumis sativus])

HSP 1 Score: 705.3 bits (1819), Expect = 6.6e-200
Identity = 364/418 (87.08%), Postives = 375/418 (89.71%), Query Frame = 1


Query: 16  VRRICLRVESGSSHVTFKAASHGCHCYF--LPIYSNSLF--LPSIHYCPPKTQNQMAFGG 75
           +RR CLRVESGSSHV FKAASHG HCYF  LPIYSNSL   LP+ HYCP KTQ QM FGG
Sbjct: 1   MRRNCLRVESGSSHVIFKAASHGFHCYFPSLPIYSNSLLFSLPT-HYCPLKTQIQMGFGG 60

Query: 76  ILGFQYGIVQAPLGPDISCPELVAAVANAGGLGLLRAPDWESPDYVRELIRKTRALTDKP 135
           +LGFQYGIVQAPLGPDIS PELVAAVANAGGLGLLRAPDWESPDYVRELIRKTRALTDKP
Sbjct: 61  LLGFQYGIVQAPLGPDISGPELVAAVANAGGLGLLRAPDWESPDYVRELIRKTRALTDKP 120

Query: 136 FGVGVILAFPHEQNLKAILDEKVAVVQVYWGECSKDLVDQVHSAGVKIIPQVGSVEEASK 195
           FG+GVILAFPHEQNLKAILDEKVAVVQVYWGECSKDLVDQVHSAGVKIIPQVGSVEEASK
Sbjct: 121 FGIGVILAFPHEQNLKAILDEKVAVVQVYWGECSKDLVDQVHSAGVKIIPQVGSVEEASK 180

Query: 196 AVEVGVDAIIVQGREAGGHVIGQEGLISILPRIVDIVGNEDIPVIAAGGISDSRGYVAAL 255
           AV+VGVDAIIVQGREAGGHVIGQEGLISILPR+V+IVG++DIPVIAAG ISDSRGYVAAL
Sbjct: 181 AVDVGVDAIIVQGREAGGHVIGQEGLISILPRVVEIVGDKDIPVIAAGSISDSRGYVAAL 240

Query: 256 ALGAHGVCLGTRLEMLNCDHSQLRKLILKSWFCSESTYKFVATEESRAHPTYKRKLVELE 315
           ALGA GVCLGTR                           FVATEES AHPTYKRKLVELE
Sbjct: 241 ALGARGVCLGTR---------------------------FVATEESNAHPTYKRKLVELE 300

Query: 316 ATDYTNIFGRARWPGAPQRVLQTPFYDDWKSLPANENESNQPVIGRSTINGLDIDIRRFA 375
           ATDYTNIFGRARWPGAPQRVLQTPFYDDWKSLPANENESNQPVIGRSTINGLDIDIRRFA
Sbjct: 301 ATDYTNIFGRARWPGAPQRVLQTPFYDDWKSLPANENESNQPVIGRSTINGLDIDIRRFA 360

Query: 376 GTVPNATTKGDIESMAMYAGQGVGLIKEILPAGEVVRRLVEGAQHLIQTHLSSFVHKE 430
           GTVPNATTKGDIESMAMYAGQGVGLIKEILPAGEVVRRLVEGAQHLI+THLSSFVHK+
Sbjct: 361 GTVPNATTKGDIESMAMYAGQGVGLIKEILPAGEVVRRLVEGAQHLIRTHLSSFVHKD 390

[Close]

BLAST of Lsi03G007410 vs. NCBI nr
Match: gi|659110334|ref|XP_008455173.1| (PREDICTED: nitronate monooxygenase isoform X1 [Cucumis melo])

HSP 1 Score: 636.0 bits (1639), Expect = 4.9e-179
Identity = 323/363 (88.98%), Postives = 331/363 (91.18%), Query Frame = 1


Query: 67  MAFGGILGFQYGIVQAPLGPDISCPELVAAVANAGGLGLLRAPDWESPDYVRELIRKTRA 126
           M+FGG+LGFQYGIVQAPLGPDIS PELVAAVANAGGLGLLRAPDWESPDYVRELIRKTRA
Sbjct: 1   MSFGGLLGFQYGIVQAPLGPDISGPELVAAVANAGGLGLLRAPDWESPDYVRELIRKTRA 60

Query: 127 LTDKPFGVGVILAFPHEQNLKAILDEKVAVVQVYWGECSKDLVDQVHSAGVKIIPQVGSV 186
           LTDKPFG+GVILAFPHEQNLKAILDEKVAVVQVYWGECSKDLVDQVHSAGVKIIPQVGSV
Sbjct: 61  LTDKPFGIGVILAFPHEQNLKAILDEKVAVVQVYWGECSKDLVDQVHSAGVKIIPQVGSV 120

Query: 187 EEASKAVEVGVDAIIVQGREAGGHVIGQEGLISILPRIVDIVGNEDIPVIAAGGISDSRG 246
           EEASKAV+ GVDAIIVQGREAGGHVIGQEGLISILPR+V+IVGNEDIPVIAAG ISDSRG
Sbjct: 121 EEASKAVDAGVDAIIVQGREAGGHVIGQEGLISILPRVVEIVGNEDIPVIAAGSISDSRG 180

Query: 247 YVAALALGAHGVCLGTRLEMLNCDHSQLRKLILKSWFCSESTYKFVATEESRAHPTYKRK 306
           YVAALALGA GVCLGTR                           FVATEES AHPTYKRK
Sbjct: 181 YVAALALGARGVCLGTR---------------------------FVATEESHAHPTYKRK 240

Query: 307 LVELEATDYTNIFGRARWPGAPQRVLQTPFYDDWKSLPANENESNQPVIGRSTINGLDID 366
           LVE+EATDYTNIFGRARWPGAPQRVLQTPFYDDWKSLPANENESNQPVIGRSTINGLDID
Sbjct: 241 LVEMEATDYTNIFGRARWPGAPQRVLQTPFYDDWKSLPANENESNQPVIGRSTINGLDID 300

Query: 367 IRRFAGTVPNATTKGDIESMAMYAGQGVGLIKEILPAGEVVRRLVEGAQHLIQTHLSSFV 426
           IRRFAGTVPNATTKGDIESMAMYAGQGVGLIKEILPAGEVVRRLVEGAQHLIQTHLSSFV
Sbjct: 301 IRRFAGTVPNATTKGDIESMAMYAGQGVGLIKEILPAGEVVRRLVEGAQHLIQTHLSSFV 336

Query: 427 HKE 430
           HK+
Sbjct: 361 HKD 336

[Close]

BLAST of Lsi03G007410 vs. NCBI nr
Match: gi|1012250541|ref|XP_015943467.1| (PREDICTED: nitronate monooxygenase [Arachis duranensis])

HSP 1 Score: 532.3 bits (1370), Expect = 7.6e-148
Identity = 261/357 (73.11%), Postives = 300/357 (84.03%), Query Frame = 1


Query: 67  MAFGGILGFQYGIVQAPLGPDISCPELVAAVANAGGLGLLRAPDWESPDYVRELIRKTRA 126
           M + G+LGF+YGIVQAPLGPDIS PELVAAVANAGGLGLLRAPDWESPDYVR LI KTR+
Sbjct: 1   MGWRGLLGFEYGIVQAPLGPDISGPELVAAVANAGGLGLLRAPDWESPDYVRGLINKTRS 60

Query: 127 LTDKPFGVGVILAFPHEQNLKAILDEKVAVVQVYWGECSKDLVDQVHSAGVKIIPQVGSV 186
           LTDKPFG+GV+LAFPHEQNLKAILDEKVA++QVYWG+C+ DLV   HSAGVKI+PQVG +
Sbjct: 61  LTDKPFGIGVVLAFPHEQNLKAILDEKVAILQVYWGDCTPDLVAMAHSAGVKIVPQVGDL 120

Query: 187 EEASKAVEVGVDAIIVQGREAGGHVIGQEGLISILPRIVDIVGNEDIPVIAAGGISDSRG 246
             A +A+  GVD IIVQGREAGGHVIGQ+GLIS++P +VD+VGN +IPVIAAGGI D+RG
Sbjct: 121 RGAKQAINAGVDGIIVQGREAGGHVIGQDGLISLVPTVVDLVGNRNIPVIAAGGIVDARG 180

Query: 247 YVAALALGAHGVCLGTRLEMLNCDHSQLRKLILKSWFCSESTYKFVATEESRAHPTYKRK 306
           YVAALALGA GVCLGTR                           FVATEES AHPTYKRK
Sbjct: 181 YVAALALGAQGVCLGTR---------------------------FVATEESYAHPTYKRK 240

Query: 307 LVELEATDYTNIFGRARWPGAPQRVLQTPFYDDWKSLPANENESNQPVIGRSTINGLDID 366
           L+E++ T+YT++FGRARWPGAPQRVL+TPFY DW+SLP++ENE+NQP+IG STI+G+D +
Sbjct: 241 LIEMDETEYTDVFGRARWPGAPQRVLKTPFYKDWRSLPSHENETNQPLIGHSTIHGMDRE 300

Query: 367 IRRFAGTVPNATTKGDIESMAMYAGQGVGLIKEILPAGEVVRRLVEGAQHLIQTHLS 424
           IRRFAGTVPN TT GDIESMAMYAGQGVGLIKEILPAGEVV+RLVEGAQ LI+ H +
Sbjct: 301 IRRFAGTVPNMTTTGDIESMAMYAGQGVGLIKEILPAGEVVKRLVEGAQILIKQHFN 330

[Close]

BLAST of Lsi03G007410 vs. NCBI nr
Match: gi|224140777|ref|XP_002323755.1| (hypothetical protein POPTR_0017s07870g [Populus trichocarpa])

HSP 1 Score: 531.2 bits (1367), Expect = 1.7e-147
Identity = 266/353 (75.35%), Postives = 296/353 (83.85%), Query Frame = 1


Query: 67  MAFGGILGFQYGIVQAPLGPDISCPELVAAVANAGGLGLLRAPDWESPDYVRELIRKTRA 126
           M + GILGF+YGIVQAPLGPDIS PELVAAVANAGGLGLLRAPDWESPDY+RELIRKTR+
Sbjct: 1   MGWRGILGFEYGIVQAPLGPDISGPELVAAVANAGGLGLLRAPDWESPDYLRELIRKTRS 60

Query: 127 LTDKPFGVGVILAFPHEQNLKAILDEKVAVVQVYWGECSKDLVDQVHSAGVKIIPQVGSV 186
           LTDKPFGVG++LAFPHE+NLK ILDEKVAV+QVYWGECSK+LV + H AGVK++PQVGS 
Sbjct: 61  LTDKPFGVGLVLAFPHEENLKVILDEKVAVLQVYWGECSKELVLEAHLAGVKVVPQVGSF 120

Query: 187 EEASKAVEVGVDAIIVQGREAGGHVIGQEGLISILPRIVDIVGNEDIPVIAAGGISDSRG 246
           E+A KA++ GVD IIVQGREAGGHVIGQEGLISILPR+VD+VG+ DIPVIAAGGI ++RG
Sbjct: 121 EDARKAIDAGVDGIIVQGREAGGHVIGQEGLISILPRVVDLVGDRDIPVIAAGGIVNARG 180

Query: 247 YVAALALGAHGVCLGTRLEMLNCDHSQLRKLILKSWFCSESTYKFVATEESRAHPTYKRK 306
           YVAALALGA GVCLGTR                           FVATEES AHPTYKRK
Sbjct: 181 YVAALALGAKGVCLGTR---------------------------FVATEESNAHPTYKRK 240

Query: 307 LVELEATDYTNIFGRARWPGAPQRVLQTPFYDDWKSLPANENESNQPVIGRSTINGLDID 366
           LVEL+ T+YTNIFGRARWP APQR L TPF++DWKSLP +ENE NQPVIGRSTING + +
Sbjct: 241 LVELDRTEYTNIFGRARWPNAPQRALVTPFFNDWKSLPPHENEINQPVIGRSTINGEEKE 300

Query: 367 IRRFAGTVPNATTKGDIESMAMYAGQGVGLIKEILPAGEVVRRLVEGAQHLIQ 420
           IRRF G VPN TT GDIESMAMYAGQGVGLIKEI PA EV++RLVEGAQ LI+
Sbjct: 301 IRRFGGPVPNMTTTGDIESMAMYAGQGVGLIKEIWPASEVIKRLVEGAQQLIR 326

[Close]

BLAST of Lsi03G007410 vs. NCBI nr
Match: gi|743899655|ref|XP_011043115.1| (PREDICTED: nitronate monooxygenase [Populus euphratica])

HSP 1 Score: 528.5 bits (1360), Expect = 1.1e-146
Identity = 265/353 (75.07%), Postives = 295/353 (83.57%), Query Frame = 1


Query: 67  MAFGGILGFQYGIVQAPLGPDISCPELVAAVANAGGLGLLRAPDWESPDYVRELIRKTRA 126
           M + GILGF+YGIVQAPLGPDIS PELVAAVANAGGLGLLRAPDWESPDY+RELIRKTR+
Sbjct: 1   MGWRGILGFEYGIVQAPLGPDISGPELVAAVANAGGLGLLRAPDWESPDYLRELIRKTRS 60

Query: 127 LTDKPFGVGVILAFPHEQNLKAILDEKVAVVQVYWGECSKDLVDQVHSAGVKIIPQVGSV 186
           LTDKPFGVG++LAFPHE+NLK ILDEKVAV+QVYWGECSK+LV + H AGVK++PQVGS 
Sbjct: 61  LTDKPFGVGLVLAFPHEENLKVILDEKVAVLQVYWGECSKELVLEAHLAGVKVVPQVGSF 120

Query: 187 EEASKAVEVGVDAIIVQGREAGGHVIGQEGLISILPRIVDIVGNEDIPVIAAGGISDSRG 246
           E+A KA++ GVD IIVQGREAGGHVIGQEGLISILPR+VD+VG+ DIPVIAAGGI ++RG
Sbjct: 121 EDARKAIDAGVDGIIVQGREAGGHVIGQEGLISILPRVVDLVGDRDIPVIAAGGIVNARG 180

Query: 247 YVAALALGAHGVCLGTRLEMLNCDHSQLRKLILKSWFCSESTYKFVATEESRAHPTYKRK 306
           YVAALALGA GVCLGTR                           FVATEES AHPTYKRK
Sbjct: 181 YVAALALGAKGVCLGTR---------------------------FVATEESNAHPTYKRK 240

Query: 307 LVELEATDYTNIFGRARWPGAPQRVLQTPFYDDWKSLPANENESNQPVIGRSTINGLDID 366
           LVEL+ T YTNIFGRARWP APQR L TPF++DWKSLP +ENE NQPVIGRSTING + +
Sbjct: 241 LVELDRTQYTNIFGRARWPNAPQRALVTPFFNDWKSLPPHENEINQPVIGRSTINGEEKE 300

Query: 367 IRRFAGTVPNATTKGDIESMAMYAGQGVGLIKEILPAGEVVRRLVEGAQHLIQ 420
           I RF G+VPN TT GDIESMAMYAGQGVGLIKEI PA EV++RLVEGAQ LI+
Sbjct: 301 IWRFGGSVPNVTTTGDIESMAMYAGQGVGLIKEIWPASEVIKRLVEGAQQLIR 326

The following BLAST results are available for this feature:

BLAST of Lsi03G007410 vs. Swiss-Prot


Match Name	E-value	Identity	Description
2NPD_NEUCR	4.5e-23	27.37	Nitronate monooxygenase OS=Neurospora crassa (strain ATCC 24698 / 74-OR23-1A / C...	[more]
2NPD_STAA3	6.5e-22	33.47	Probable nitronate monooxygenase OS=Staphylococcus aureus (strain USA300) GN=SAU...	[more]
2NPD_STAA8	6.5e-22	33.47	Probable nitronate monooxygenase OS=Staphylococcus aureus (strain NCTC 8325) GN=...	[more]
2NPD_STAAC	6.5e-22	33.47	Probable nitronate monooxygenase OS=Staphylococcus aureus (strain COL) GN=SACOL0...	[more]
2NPD_STAAE	6.5e-22	33.47	Probable nitronate monooxygenase OS=Staphylococcus aureus (strain Newman) GN=NWM...	[more]

BLAST of Lsi03G007410 vs. TrEMBL


Match Name	E-value	Identity	Description
A0A0A0K422_CUCSA	4.6e-200	87.08	Uncharacterized protein OS=Cucumis sativus GN=Csa_7G064010 PE=4 SV=1	[more]
B9IJE3_POPTR	1.2e-147	75.35	Uncharacterized protein OS=Populus trichocarpa GN=POPTR_0017s07870g PE=4 SV=1	[more]
A0A067L9X1_JATCU	5.5e-145	73.46	Uncharacterized protein OS=Jatropha curcas GN=JCGZ_24913 PE=4 SV=1	[more]
A0A061E2B3_THECC	1.6e-144	71.99	Aldolase-type TIM barrel family protein isoform 1 OS=Theobroma cacao GN=TCM_0074...	[more]
W9SAP4_9ROSA	2.1e-144	71.99	Putative nitronate monooxygenase OS=Morus notabilis GN=L484_010808 PE=4 SV=1	[more]

BLAST of Lsi03G007410 vs. TAIR10


Match Name	E-value	Identity	Description
AT5G64250.2	7.6e-146	72.41	Aldolase-type TIM barrel family protein	[more]

BLAST of Lsi03G007410 vs. NCBI nr


Match Name	E-value	Identity	Description
gi\|778724449\|ref\|XP_011658807.1\|	6.6e-200	87.08	PREDICTED: nitronate monooxygenase isoform X1 [Cucumis sativus]	[more]
gi\|659110334\|ref\|XP_008455173.1\|	4.9e-179	88.98	PREDICTED: nitronate monooxygenase isoform X1 [Cucumis melo]	[more]
gi\|1012250541\|ref\|XP_015943467.1\|	7.6e-148	73.11	PREDICTED: nitronate monooxygenase [Arachis duranensis]	[more]
gi\|224140777\|ref\|XP_002323755.1\|	1.7e-147	75.35	hypothetical protein POPTR_0017s07870g [Populus trichocarpa]	[more]
gi\|743899655\|ref\|XP_011043115.1\|	1.1e-146	75.07	PREDICTED: nitronate monooxygenase [Populus euphratica]	[more]

Annotated Terms

The following terms have been associated with this gene:

Vocabulary: **Molecular Function**
Term	Definition
GO:0003824	catalytic activity
GO:0018580	nitronate monooxygenase activity

Vocabulary: **Biological Process**
Term	Definition
GO:0055114	oxidation-reduction process

Vocabulary: **INTERPRO**
Term	Definition
IPR013785	Aldolase_TIM
IPR004136	NMO

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0006807	nitrogen compound metabolic process
biological_process	GO:0055114	oxidation-reduction process
biological_process	GO:0046482	para-aminobenzoic acid metabolic process
biological_process	GO:0006310	DNA recombination
biological_process	GO:0006302	double-strand break repair
biological_process	GO:0046686	response to cadmium ion
biological_process	GO:0009610	response to symbiotic fungus
cellular_component	GO:0005829	cytosol
cellular_component	GO:0005634	nucleus
cellular_component	GO:0005575	cellular_component
molecular_function	GO:0051213	dioxygenase activity
molecular_function	GO:0018580	nitronate monooxygenase activity
molecular_function	GO:0003677	DNA binding
molecular_function	GO:0003824	catalytic activity

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Lsi03G007410.1	Lsi03G007410.1	mRNA

InterPro

Analysis Name: InterPro Annotations of Lagenaria siceraria
Date Performed: 2017-09-18


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR004136	Nitronate monooxygenase	PFAM	PF03060	NMO	coord: 290..412 score: 1.2E-6coord: 141..272 score: 2.3
IPR013785	Aldolase-type TIM barrel	GENE3D	G3DSA:3.20.20.70		coord: 65..264 score: 5.2E-57coord: 291..409 score: 3.7
None	No IPR available	PANTHER	PTHR32332	FAMILY NOT NAMED	coord: 64..263 score: 1.2E-134coord: 291..429 score: 1.2E
None	No IPR available	PANTHER	PTHR32332:SF20	2-NITROPROPANE DIOXYGENASE-LIKE PROTEIN	coord: 64..263 score: 1.2E-134coord: 291..429 score: 1.2E
None	No IPR available	unknown	SSF51412	Inosine monophosphate dehydrogenase (IMPDH)	coord: 290..408 score: 7.55E-52coord: 51..262 score: 7.55

Synteny

The following gene(s) are orthologous to this gene:


Gene	Orthologue	Organism	Block
Lsi03G007410	Csa7G064010	Cucumber (Chinese Long) v2	culsiB522
Lsi03G007410	CSPI07G05730	Wild cucumber (PI 183967)	cpilsiB536
Lsi03G007410	Cucsa.218110	Cucumber (Gy14) v1	cgylsiB367
Lsi03G007410	CmaCh11G016550	Cucurbita maxima (Rimu)	cmalsiB117
Lsi03G007410	CmaCh19G004650	Cucurbita maxima (Rimu)	cmalsiB484
Lsi03G007410	CmoCh19G004810	Cucurbita moschata (Rifu)	cmolsiB468
Lsi03G007410	Cla012877	Watermelon (97103) v1	lsiwmB233
Lsi03G007410	MELO3C018623	Melon (DHL92) v3.5.1	lsimeB247
Lsi03G007410	ClCG09G015620	Watermelon (Charleston Gray)	lsiwcgB215
Lsi03G007410	MELO3C018623.2	Melon (DHL92) v3.6.1	lsimedB260
Lsi03G007410	Cla97C09G177590	Watermelon (97103) v2	lsiwmbB251
Lsi03G007410	Bhi05G000730	Wax gourd	lsiwgoB320
Lsi03G007410	Cp4.1LG04g07090	Cucurbita pepo (Zucchini)	cpelsiB544
Lsi03G007410	Cp4.1LG15g03680	Cucurbita pepo (Zucchini)	cpelsiB208
Lsi03G007410	CsGy7G005450	Cucumber (Gy14) v2	cgyblsiB489
Lsi03G007410	Carg22823	Silver-seed gourd	carlsiB262
Lsi03G007410	Carg26460	Silver-seed gourd	carlsiB338
Lsi03G007410	CsaV3_7G006720	Cucumber (Chinese Long) v3	cuclsiB555

The following gene(s) are paralogous to this gene:

None

The following block(s) are covering this gene:


Gene	Organism	Block
Lsi03G007410	Cucurbita maxima (Rimu)	cmalsiB482
Lsi03G007410	Cucurbita moschata (Rifu)	cmolsiB104
Lsi03G007410	Cucurbita moschata (Rifu)	cmolsiB466
Lsi03G007410	Cucurbita pepo (Zucchini)	cpelsiB206
Lsi03G007410	Silver-seed gourd	carlsiB888