Cp4.1LG15g03680 (gene) Cucurbita pepo (Zucchini) | Cucurbit Genomics Database (CuGenDB)

Overview


Name	Cp4.1LG15g03680
Type	gene
Organism	Cucurbita pepo (Cucurbita pepo (Zucchini))
Description	2-nitropropane dioxygenase, putative
Location	Cp4.1LG15 : 4824025 .. 4829328 (-)

Genome Viewer

Gene Expression

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: five_prime_UTRCDSthree_prime_UTR

Hold the cursor over a type above to highlight its positions in the sequence below.

CGGATTAAAGCGAGTGTGCGGCGTATTTGCTTACGTGTTGAACCCGGCAGCTCTCACGTAACCTTCAAAGCCGCCTCTCCCATGGCTGCCACTGCCACTTCCTTCCGGTGTTTTAAAATTGGCTCTTCTTCCAATCAATCCATCATTGCCCATCCAAGAGACAGAATCAGATGGATTCTGGAGGGATTCTCGGTTTCCAATACGGCATAGTTCAGGCTCCGCTTGGACCTGATATCTCCGGTCCTGAGCTTGTTGCTGCGGTCGCCAATGCCGGCGGACTCGGTCTTCTCAGATCCCCCGATTGGGTAACTTACTGTCTATTATTCCTTATTGTTCAATTAGTTTTGTTGGAAATGAATGTAATCTGTTGTTGCGAAGCATCATCATCTGAGTTTCTCGTTTTGTAATGGATTCTGCTATTGATTGTTCGATCATATGTATGTCGTCGTCGGATTTTGGAACTTTTAACTGTCGATTAGTTTTATAGATTGGCGTTTTCTGATTTTTGCAACTGCTTATTGATTGTTTTTAAAGGAATGCGGACAATTTGGTGGTGATTTGTTGTGAAATTTTGGAGGAGTGTTGGGTTTAATGGGGACTTAGTCCGGGGAATCGATTATGTGAGTGAACAGTTGAAATATTGACGTTGTCTATCTGTTCATGTGGGCACTTTGTATTTGTTATGAATGTAAAGATTTCATTGAAACCTTCTTTTCCTTCGTTCTTTTATTAGTCTAGAAATTGTTTATAAAATTTGAGTATCAACGTGAGATCCCACATTGGGAGAAGAACGAAACATTCTTTATAAGGGTGTGAAAACCTCTCCCTAGTAGACGTGTTTTAAAAACCATGGAGGGAAAGCCCAAAAGGGAAAACCCAAAGAGGACAATATCTGCTAGCGGTGGGCTTGGGCTGTTACAGTTGGTATTAGAGCCAGGCACCGAGCGGTGTGTCGCCGAGGACACTGGGCTACGAAGGGGGTTCCCACATCGATTGGAGAAGGGAACGAGTGCTAGTGAGGACGCAACATTCTTTATAAGGGGGCTCCGAAAGGGGGTAGATTGTGTGATTCCACATCAGTTGGGGAGGAGAACGAAGCATTCTTTATAAGGGTGTGGAAACCTCTCCCTAGCAGTCGCGTTTTAAAAACCTTAAGGGAAAGCCCAAAGAGGACAATATCGGCNTCTGATTTTTGCAACTGCTTATTGATTGTTTTTAAAGGAATGCGGACAATTTGGTGGTGATTTGTTGTGAAATTTTGGAGGAGTGTTGGGTTTAATGGGGACTTAGTCCGGGGAATCGATTATGTGAGTGAACAGTTGAAATATTGACGTTGTCTATCTGTTCATGTGGGCACTTTGTATTTGTTATGAATGTAAAGATTTCATTGAAACCTTCTTTTCCTTCGTTCTTTTATTAGTCTAGAAATTGTTTATAAAATTTGAGTATCAACGTGAGATCCCACATTGGGAGAAGAACGAAACATTCTTTATAAGGGTGTGAAAACCTCTCCCTAGTAGACGTGTTTTAAAAACCATGGAGGGAAAGCCCAAAAGGGAAAACCCAAAGAGGACAATATCTGCTAGCGGTGGGCTTGGGCTGTTACAGTTGGTATTAGAGCCAGGCACCGAGCGGTGTGTCGCCGAGGACACTGGGCTACGAAGGGGGTTCCCACATCGATTGGAGAAGGGAACGAGTGCTAGTGAGGACGCAACATTCTTTATAAGGGGGCTCCGAAAGGGGGTAGATTGTGTGATTCCACATCAGTTGGGGAGGAGAACGAAGCATTCTTTATAAGGGTGTGGAAACCTCTCCCTAGCAGTCGCGTTTTAAAAACCTTAAGGGAAAGCCCAAAGAGGACAATATCGGCTTAGCGGTGGGCTTGGGCTTGGGCTTGGGCTTGGGCTTGGGCTTGGGCTGTTACAATCAGTTTCATAACCATTTAGTCTTTGTTGCCGAAGAGCTAATGTGCTGCAGTATTTAGCTTATGTTTCTACTTTGTGCAATGGTTTTGAGATTTTAGCCAAATTTTAAAAAATCAATCAAATTTCTGGGAACTACATGTTTTTGGTTTGTGAATCTTGCTTTGATCTAAAAAATATATGCATGTATTCTGCACTGAACAGGCTTATCTTCTTATATTGAAAACATACAGTAAATTGATGATCAAATATAATGTCAAAAGGTGTATAGCTTGCTTCTCTTTTGTCCCAACTATTTATTTACATCTTTTCCTGTTTTCCACTCTAACCGAGTAGCATGGAGCTCATTTTATACGTACCAGGAATCGCCTGATTATGTACGAGAATTGATACGGAAGACCCGAGCATTAACAGATAAACCATTTGGAGTAGGTGTAATTTTAGCATTTCCCCATGAGCAAAATCTAAAGGCCATATTAGATGAGAAAGTAGCAGTTCTGCAAGTTTACTGGGGAGAATGTTCAAAGGCTCTTGTGGACCAAGCTCATTCTGCTGGGGTGAAAATTATTCCACAAGTGAGTGTTTTCTTGCAAATCACATCAACAATGCGCGGCCACACGACCCAATTCAAGTAGATTTTTTCATTTTATTATTAAACTTATTATTGTTCCAAGAATTTTTATGCGCATGGATGTGGAAGCGTTATCTGAAATCACCTTCAGGTGGTTCATTATTATGTTCTATGACGTTATCGGTCCTCTGTTCATATTGTGGTATGTTACAATTTTTAAAAACTACTTTTGCAGGTTGGGAGCGTCGAAGAAGCAAGCAAAGCAGTTGATGTTGGTGTTGATGCAATAATCGTGCAAGGTCGGGAAGCGGGAGGGCATGTTATTGGTCAAGTATAGACCTTCTTCTTCCCAGAAAACTTATTTAATTTGTGGTGCTATTGTGTTTGTTTCTAGGCTTCTTCCATCATAGTTTCTTTTTCATTTGCTAAATCGCTCGTGGGCTATGTTTTTGCACGGTTCTTCTCCTTCGGAAAAAACTTCTCTAAATTACTAGAAAATTAAAGGAAAAGCATCTGTTTCTCAATTCATTAAGAAACTGTTGTCGTAGTTCAGGTATCTCCTGACTTACCTGCTCCAAGTTCATCGTTCGAAGGGTATCGAGTTTGAAATCCCCTTGGAGAGAACTAGATTTTTTAATATTAATCTTTATCATAAAAGTATTTGGACATTCTTCCTTTTTGACATTAATCATGCATATACATTTCGATTACTTGCTACTCTTCTTCTTTAAATTTCACTATATTGTATGAACTGCTGGTGTTTTTATTTGTTGCTTCCAGTCAGTGATACTCATTTGGATCATGTTTTCATTAGTCATGTTCTTCACCTCTCTTCACATTTGTATTTCAACTATATCGTAACTTTTCTCAAATCATTATTTGAAAAAATGAAAATAGTCTACAGAAGTTTGACTTGATTCTTTACAGGAAGGTTTGATTTCCATCTTACCAAGGGTAGTTGATATTGTTGGGAATGAAGATATACCTATAATTGCAGCTGGTGGTATTTCAGATTCTCGAGGTTATGTTGCTGCCCTGGCCCTGGGCGCTCGTGGTGTCTGCCTCGGAACTAGGTTAGAAAAAGCTGAACTATGTCTGTTCGACTCGGAAAACATGTTTTTAAAAGTTGGCTGATCCATGCTACAAAATGGTGCTCAATTCGAAACAATACCCGGTATCGAAAATAGAATACTGTCGCACGTTCAGAGGCAGAAAGCCTTTTTTTCTTTGGTGTAATCCAAAAGTTGCTGTTATCCTTGCTATATCATATAATCTAATAACAAAACATGTCTCATAAATTTTAGATAGTATCACCATTGATCTTTTGCATCTTCTGAGACTTAAATTTTACTTTTTAAATGTTTGTTGTGAGAGTAATTTGAGTACGAAGAACTAAATGAAATATTGCAGATTTGTTGCTACTGAAGAAAGCCATGCTCACCCTGCATACAAGAGGAAGTTGGTAGAACTGGACGCAACTGATTATACGAACATTTTTGGGCGTGCAAGATGGCCCGGCGCACCCCAGCGTGTTCTACAGACACCCTTTTACGATGATTGGAAGTCGCTTCCTGCCAACGAAAACGAAAGCAATCAGCCTGTCATTGGCCGCTCAACAATTAATGGCTTGGTGAGCTCCCCCACCTTATTTCTTTTCTACTTAAACAGATGCATAAAAGAGATATTTATCTTCCATGATGTGAGATCCCACCTCGGTTAGGGAGGAAAACGAAGCATTCTTTTTAAGGGTGTGGAAATCTCTCCCTTGCAGATGTATTTTAAAAACCTTGAGGGGAAGCCCGAAACGAAAAGCCCAAGGAGGACAATATAGGTTAGCGGTGGGCTTGGACCTTTACAAATGGTATTAGAGCCAAGTTTTGGGCGTCAACGAGGAGGCTAAACCCCAAACTGGGGGTGGACACAAGGTAGTGTGTCAGCGAGGAGGCTAAGCCCTGAAGCGGGTGGACACGAGGCAGTATGCCAGCAAGGAGAAGGGAAGTAGTGAGGGTGGATTGGAGGGTCCCACATCGATTGGAGAAGAGAACGAGTGTCAGCAAGCATGTTGAGCCCTGAATGGGTGGATTATGAGATCTCACATTGGTTGAGGAGGAGAACGAAACATTATTTATAAGGATGTGGAAACCTCTCCCTAGCAAGCTTGTTTTAAAAACCTTGAGGGGAAGCCCGAAAGAGAAAGTCTAATGAGAACAATATCTGCTAGCGGTGGACTGTTACACAGACATACATTAAAAAAATTAAAAAAAAAAGTTAGCTGCCATTTATTCTTCATGCTTGAAGAAACTCTGCAGTACTAAATATTTATGTTGAAAGTGAAGAAGTTAAGGATCTTCTCTTGTTCTTCTCTACATTAACATTCTTCATTTAATACATTCATGCTCGGAACAGGACATAGATGTACGGCGCTTCGCAGGAACCGTGCCAAACGCAACGACAAAAGGCGACATCGAGAGCATGGCAATGTATGCGGGGCAAGGAGTAGGACTAATCAAGGAGATACTACCAGCAGGAGAAGTGGTAAGAAAGCTGGTTGAAGGTGCTCAACATCTGATCCAAACACATTTGAGTAGTTTTGTTCATAAAGATTGATGCTCAGAGCTGCAATATTTTTTGTTTCCTGAAAGAAATAAGTTCAAAAATCCATATGTAGATGTAGATGAATGTCTGAAAAATTGAGCTTCACAAGTTAAATAAAAGTAGTAACTGGGATTTGCTGTTTTGTTCGCATCAGTATCAGTGCCATTTCAGGTCCTTATATCTTTAAAGCTTGTCTGGATTGATATCTTTTATGTT

mRNA sequence

CGGATTAAAGCGAGTGTGCGGCGTATTTGCTTACGTGTTGAACCCGGCAGCTCTCACGTAACCTTCAAAGCCGCCTCTCCCATGGCTGCCACTGCCACTTCCTTCCGGTGTTTTAAAATTGGCTCTTCTTCCAATCAATCCATCATTGCCCATCCAAGAGACAGAATCAGATGGATTCTGGAGGGATTCTCGGTTTCCAATACGGCATAGTTCAGGCTCCGCTTGGACCTGATATCTCCGGTCCTGAGCTTGTTGCTGCGGTCGCCAATGCCGGCGGACTCGGTCTTCTCAGATCCCCCGATTGGGAATCGCCTGATTATGTACGAGAATTGATACGGAAGACCCGAGCATTAACAGATAAACCATTTGGAGTAGGTGTAATTTTAGCATTTCCCCATGAGCAAAATCTAAAGGCCATATTAGATGAGAAAGTAGCAGTTCTGCAAGTTTACTGGGGAGAATGTTCAAAGGCTCTTGTGGACCAAGCTCATTCTGCTGGGGTGAAAATTATTCCACAAGTTGGGAGCGTCGAAGAAGCAAGCAAAGCAGTTGATGTTGGTGTTGATGCAATAATCGTGCAAGGTCGGGAAGCGGGAGGGCATGTTATTGGTCAAGAAGGTTTGATTTCCATCTTACCAAGGGTAGTTGATATTGTTGGGAATGAAGATATACCTATAATTGCAGCTGGTGGTATTTCAGATTCTCGAGGTTATGTTGCTGCCCTGGCCCTGGGCGCTCGTGGTGTCTGCCTCGGAACTAGATTTGTTGCTACTGAAGAAAGCCATGCTCACCCTGCATACAAGAGGAAGTTGGTAGAACTGGACGCAACTGATTATACGAACATTTTTGGGCGTGCAAGATGGCCCGGCGCACCCCAGCGTGTTCTACAGACACCCTTTTACGATGATTGGAAGTCGCTTCCTGCCAACGAAAACGAAAGCAATCAGCCTGTCATTGGCCGCTCAACAATTAATGGCTTGGACATAGATGTACGGCGCTTCGCAGGAACCGTGCCAAACGCAACGACAAAAGGCGACATCGAGAGCATGGCAATGTATGCGGGGCAAGGAGTAGGACTAATCAAGGAGATACTACCAGCAGGAGAAGTGGTAAGAAAGCTGGTTGAAGGTGCTCAACATCTGATCCAAACACATTTGAGTAGTTTTGTTCATAAAGATTGATGCTCAGAGCTGCAATATTTTTTGTTTCCTGAAAGAAATAAGTTCAAAAATCCATATGTAGATGTAGATGAATGTCTGAAAAATTGAGCTTCACAAGTTAAATAAAAGTAGTAACTGGGATTTGCTGTTTTGTTCGCATCAGTATCAGTGCCATTTCAGGTCCTTATATCTTTAAAGCTTGTCTGGATTGATATCTTTTATGTT

Coding sequence (CDS)

ATGGATTCTGGAGGGATTCTCGGTTTCCAATACGGCATAGTTCAGGCTCCGCTTGGACCTGATATCTCCGGTCCTGAGCTTGTTGCTGCGGTCGCCAATGCCGGCGGACTCGGTCTTCTCAGATCCCCCGATTGGGAATCGCCTGATTATGTACGAGAATTGATACGGAAGACCCGAGCATTAACAGATAAACCATTTGGAGTAGGTGTAATTTTAGCATTTCCCCATGAGCAAAATCTAAAGGCCATATTAGATGAGAAAGTAGCAGTTCTGCAAGTTTACTGGGGAGAATGTTCAAAGGCTCTTGTGGACCAAGCTCATTCTGCTGGGGTGAAAATTATTCCACAAGTTGGGAGCGTCGAAGAAGCAAGCAAAGCAGTTGATGTTGGTGTTGATGCAATAATCGTGCAAGGTCGGGAAGCGGGAGGGCATGTTATTGGTCAAGAAGGTTTGATTTCCATCTTACCAAGGGTAGTTGATATTGTTGGGAATGAAGATATACCTATAATTGCAGCTGGTGGTATTTCAGATTCTCGAGGTTATGTTGCTGCCCTGGCCCTGGGCGCTCGTGGTGTCTGCCTCGGAACTAGATTTGTTGCTACTGAAGAAAGCCATGCTCACCCTGCATACAAGAGGAAGTTGGTAGAACTGGACGCAACTGATTATACGAACATTTTTGGGCGTGCAAGATGGCCCGGCGCACCCCAGCGTGTTCTACAGACACCCTTTTACGATGATTGGAAGTCGCTTCCTGCCAACGAAAACGAAAGCAATCAGCCTGTCATTGGCCGCTCAACAATTAATGGCTTGGACATAGATGTACGGCGCTTCGCAGGAACCGTGCCAAACGCAACGACAAAAGGCGACATCGAGAGCATGGCAATGTATGCGGGGCAAGGAGTAGGACTAATCAAGGAGATACTACCAGCAGGAGAAGTGGTAAGAAAGCTGGTTGAAGGTGCTCAACATCTGATCCAAACACATTTGAGTAGTTTTGTTCATAAAGATTGA

Protein sequence

MDSGGILGFQYGIVQAPLGPDISGPELVAAVANAGGLGLLRSPDWESPDYVRELIRKTRALTDKPFGVGVILAFPHEQNLKAILDEKVAVLQVYWGECSKALVDQAHSAGVKIIPQVGSVEEASKAVDVGVDAIIVQGREAGGHVIGQEGLISILPRVVDIVGNEDIPIIAAGGISDSRGYVAALALGARGVCLGTRFVATEESHAHPAYKRKLVELDATDYTNIFGRARWPGAPQRVLQTPFYDDWKSLPANENESNQPVIGRSTINGLDIDVRRFAGTVPNATTKGDIESMAMYAGQGVGLIKEILPAGEVVRKLVEGAQHLIQTHLSSFVHKD

Homology

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BLAST of Cp4.1LG15g03680 vs. Swiss-Prot
Match: 2NPD_PSEAE (Nitronate monooxygenase OS=Pseudomonas aeruginosa (strain ATCC 15692 / PAO1 / 1C / PRS 101 / LMG 12228) GN=PA1024 PE=1 SV=1)

HSP 1 Score: 121.7 bits (304), Expect = 1.5e-26
Identity = 97/315 (30.79%), Postives = 149/315 (47.30%), Query Frame = 1


Query: 26  ELVAAVANAGGLGLLRSPDWESPDYVRELIRKTRALTDKPFGVGVILA-----FPHEQNL 85
           E+ AAVANAGGL  L +    SP+ +   I + R LTD+PFGV + L       P+ +  
Sbjct: 30  EMAAAVANAGGLATLSALTQPSPEALAAEIARCRELTDRPFGVNLTLLPTQKPVPYAEYR 89

Query: 86  KAILDEKVAVLQVYWGECSKALVDQAHSAGVKIIPQVGSVEEASKAVDVGVDAIIVQGRE 145
            AI++  + V++    +  + + +     GVK+I +  +V  A KA  +GVDA+ + G E
Sbjct: 90  AAIIEAGIRVVETAGNDPGEHIAEFRRH-GVKVIHKCTAVRHALKAERLGVDAVSIDGFE 149

Query: 146 AGGHVIGQEGL--ISILPRVVDIVGNEDIPIIAAGGISDSRGYVAALALGARGVCLGTRF 205
             GH  G++ +  + +LP   + +    +PIIA+GG +D RG VAALALGA  + +GTRF
Sbjct: 150 CAGHP-GEDDIPGLVLLPAAANRL---RVPIIASGGFADGRGLVAALALGADAINMGTRF 209

Query: 206 VATEESHAHPAYKRKLVELDATDYTNIFGRARWPGAPQRVLQTPFYDDWKSLPANENESN 265
           +AT E   HPA K  +   D      I    R      RV +     +  ++ A      
Sbjct: 210 LATRECPIHPAVKAAIRAADERSTDLIMRSLR---NTARVARNAISQEVLAIEA------ 269

Query: 266 QPVIGRSTINGLDIDVRRFAGTVPNATTKGDIESMAMYAGQGVGLIKEILPAGEVVRKLV 325
                R      DI              +GD +     AG   GLI +     E++R +V
Sbjct: 270 -----RGGAGYADIAALVSGQRGRQVYQQGDTDLGIWSAGMVQGLIDDEPACAELLRDIV 325

Query: 326 EGAQHLIQTHLSSFV 334
           E A+ L++  L   +
Sbjct: 330 EQARQLVRQRLEGML 325

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BLAST of Cp4.1LG15g03680 vs. Swiss-Prot
Match: 2NPD_NEUCR (Nitronate monooxygenase OS=Neurospora crassa (strain ATCC 24698 / 74-OR23-1A / CBS 708.71 / DSM 1257 / FGSC 987) GN=ncd-2 PE=1 SV=1)

HSP 1 Score: 120.9 bits (302), Expect = 2.6e-26
Identity = 104/364 (28.57%), Postives = 165/364 (45.33%), Query Frame = 1


Query: 13  IVQAPLGPDISGPELVAAVANAGGLGLLRSPDWESP--DYVREL------IRKTRALTDK 72
           I+ AP+    +G  L A V+ AGG+G +       P   ++  L       R    LTD+
Sbjct: 33  IISAPMYLIANGT-LAAEVSKAGGIGFVAGGSDFRPGSSHLTALSTELASARSRLGLTDR 92

Query: 73  PF----GVGVILAFPHEQNLKAILDEKVAVL-----QVYW----------------GECS 132
           P     G+GV L   H  ++  + D  + +L     Q  W                   +
Sbjct: 93  PLTPLPGIGVGLILTHTISVPYVTDTVLPILIEHSPQAVWLFANDPDFEASSEPGAKGTA 152

Query: 133 KALVDQAHSAGVKIIPQVGSVEEASKAVDVGVDAIIVQGREAGGHVIGQ-EGLISILPRV 192
           K +++  H++G  +  QVG+V++A KA   G D I+ QG +AGGH +    G++S++P V
Sbjct: 153 KQIIEALHASGFVVFFQVGTVKDARKAAADGADVIVAQGIDAGGHQLATGSGIVSLVPEV 212

Query: 193 VDIVG----NEDIPIIAAGGISDSRGYVAALALGARGVCLGTRFVATEESHAHPAYKRKL 252
            D++       ++ ++AAGG++D RG V AL LGA GV LGTRF    E+ + P ++RK+
Sbjct: 213 RDMLDREFKEREVVVVAAGGVADGRGVVGALGLGAEGVVLGTRFTVAVEA-STPEFRRKV 272

Query: 253 VELDATD----------YTNIFGRARWPGA-PQRVLQTPFYDDWKS-LPANENESNQPVI 312
           + L+  D          +  I     W      R ++   YDD  + +P  EN       
Sbjct: 273 I-LETNDGGLNTVKSHFHDQINCNTIWHNVYDGRAVRNASYDDHAAGVPFEENHKK---- 332

Query: 313 GRSTINGLDIDVRRFAGTVPNATTKGDIESMAMYAGQGVGLIKEILPAGEVVRKLVEGAQ 327
                                A + GD      ++G  VGLIK+  PAG++VR+L E A+
Sbjct: 333 ------------------FKEAASSGDNSRAVTWSGTAVGLIKDQRPAGDIVRELREEAK 371

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BLAST of Cp4.1LG15g03680 vs. Swiss-Prot
Match: 2NPD_STAS1 (Probable nitronate monooxygenase OS=Staphylococcus saprophyticus subsp. saprophyticus (strain ATCC 15305 / DSM 20229) GN=SSP1854 PE=3 SV=1)

HSP 1 Score: 117.1 bits (292), Expect = 3.8e-25
Identity = 99/369 (26.83%), Postives = 170/369 (46.07%), Query Frame = 1


Query: 5   GILGFQYGIVQAPLGPDISGPELVAAVANAGGLGLLRSPDWESPDYVRELIRKTRALTDK 64
           G+L  +Y I+QA +    + PELVA V+NAGGLG + +  + S D + + I   + LTD 
Sbjct: 9   GLLNVKYPIIQAGMAGSTT-PELVATVSNAGGLGTIGA-GYFSSDRLEQEITYLQELTDL 68

Query: 65  PFGVGV--------------------------------ILAFPHEQNLK----AILDEKV 124
           P+ V +                                ++    +Q  K     ++++ V
Sbjct: 69  PYAVNLFVPSDKLYIPEKVEHMNAWLKPYRRAFNIEEPVINMTEKQQFKDAIDLVIEKGV 128

Query: 125 AVLQVYWGECSKALVDQAHSAGVKIIPQVGSVEEASKAVDVGVDAIIVQGREAGGH---- 184
             +   +G   + ++++     +K+I    SVEEA      G+D +I QG EAGGH    
Sbjct: 129 PAVSFTFGIPEQTVIEKLKERHIKLIGTATSVEEAIANESAGMDMVIAQGSEAGGHRGAF 188

Query: 185 ------VIGQEGLISILPRVVDIVGNEDIPIIAAGGISDSRGYVAALALGARGVCLGTRF 244
                 +    G +S++P++VD +   +IP++AAGGI D RG VA++ LGA GV +GT F
Sbjct: 189 SETASQLTPLIGTMSLVPQMVDQI---NIPVVAAGGIMDGRGLVASMVLGAEGVQMGTAF 248

Query: 245 VATEESHAHPAYKRKLVELDATD--YTNIFGRARWPGAPQRVLQTPFYDDWKSLPANENE 304
           + ++ES A   YK  + +   TD   TN+       G P R ++  F           NE
Sbjct: 249 LTSDESGASQLYKHAISQSKETDTVVTNVI-----TGKPARGIENEFIH-------KMNE 308

Query: 305 SNQPVIGRSTINGLDIDVRRFAGTVPNATTKGDIESMAMYAGQGVGLIKEILPAGEVVRK 326
            +  +      N L   +R+       A  KG+ +   +++GQ   L++  + A  ++  
Sbjct: 309 YDDEIPDYPIQNQLTNALRK------EAANKGNAQWTHLWSGQSPRLVQH-MSAWALIEN 353

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BLAST of Cp4.1LG15g03680 vs. Swiss-Prot
Match: 2NPD_STAA3 (Probable nitronate monooxygenase OS=Staphylococcus aureus (strain USA300) GN=SAUSA300_0825 PE=3 SV=1)

HSP 1 Score: 116.3 bits (290), Expect = 6.4e-25
Identity = 101/366 (27.60%), Postives = 171/366 (46.72%), Query Frame = 1


Query: 6   ILGFQYGIVQAPLGPDISGPELVAAVANAGGLGLLRSPDWESPDYVRELIRKTRALTDKP 65
           +L  +Y I+QA +    + P+LVA+V+N+GGLG + +  + +   + + I   R LT   
Sbjct: 10  MLSIEYPIIQAGMAGSTT-PKLVASVSNSGGLGTIGA-GYFNTQQLEDEIDYVRQLTSNS 69

Query: 66  FGVGV--------------------------------ILAFPHEQNLKAILD----EKVA 125
           FGV V                                ++    EQ  K  +D    ++V 
Sbjct: 70  FGVNVFVPSQQSYTSSQIENMNAWLKPYRRALHLEEPVVKITEEQQFKCHIDTIIKKQVP 129

Query: 126 VLQVYWGECSKALVDQAHSAGVKIIPQVGSVEEASKAVDVGVDAIIVQGREAGGH----- 185
           V    +G  S+ ++ +  +A VK+I    SV+EA      G+DAI+ QG EAGGH     
Sbjct: 130 VCCFTFGIPSEQIISRLKAANVKLIGTATSVDEAIANEKAGMDAIVAQGSEAGGHRGSFL 189

Query: 186 ----VIGQEGLISILPRVVDIVGNEDIPIIAAGGISDSRGYVAALALGARGVCLGTRFVA 245
                +   G IS++P++VD+V    IP+IAAGGI D RG +A++ LGA GV +GT F+ 
Sbjct: 190 KPKNQLPMVGTISLVPQIVDVV---SIPVIAAGGIMDGRGVLASIVLGAEGVQMGTAFLT 249

Query: 246 TEESHAHPAYKRKLVELDATDYTNIFGRARWPGAPQRVLQTPFYDDWKSLPANENESNQP 305
           +++S+A    +  ++    TD   +  +A + G   R +   F ++      ++ E + P
Sbjct: 250 SQDSNASELLRDAIINSKETD--TVITKA-FSGKLARGINNRFIEE-----MSQYEGDIP 309

Query: 306 VIGRSTINGLDIDVRRFAGTVPNATTKGDIESMAMYAGQGVGLIKEILPAGEVVRKLVEG 327
                  N L   +R+ A  +      GD E + M++GQ   L     PA  ++  ++  
Sbjct: 310 --DYPIQNELTSSIRKAAANI------GDKELIHMWSGQSPRL-ATTHPANTIMSNIINQ 353

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BLAST of Cp4.1LG15g03680 vs. Swiss-Prot
Match: 2NPD_STAA8 (Probable nitronate monooxygenase OS=Staphylococcus aureus (strain NCTC 8325) GN=SAOUHSC_00855 PE=3 SV=1)

HSP 1 Score: 116.3 bits (290), Expect = 6.4e-25
Identity = 101/366 (27.60%), Postives = 171/366 (46.72%), Query Frame = 1


Query: 6   ILGFQYGIVQAPLGPDISGPELVAAVANAGGLGLLRSPDWESPDYVRELIRKTRALTDKP 65
           +L  +Y I+QA +    + P+LVA+V+N+GGLG + +  + +   + + I   R LT   
Sbjct: 10  MLSIEYPIIQAGMAGSTT-PKLVASVSNSGGLGTIGA-GYFNTQQLEDEIDYVRQLTSNS 69

Query: 66  FGVGV--------------------------------ILAFPHEQNLKAILD----EKVA 125
           FGV V                                ++    EQ  K  +D    ++V 
Sbjct: 70  FGVNVFVPSQQSYTSSQIENMNAWLKPYRRALHLEEPVVKITEEQQFKCHIDTIIKKQVP 129

Query: 126 VLQVYWGECSKALVDQAHSAGVKIIPQVGSVEEASKAVDVGVDAIIVQGREAGGH----- 185
           V    +G  S+ ++ +  +A VK+I    SV+EA      G+DAI+ QG EAGGH     
Sbjct: 130 VCCFTFGIPSEQIISRLKAANVKLIGTATSVDEAIANEKAGMDAIVAQGSEAGGHRGSFL 189

Query: 186 ----VIGQEGLISILPRVVDIVGNEDIPIIAAGGISDSRGYVAALALGARGVCLGTRFVA 245
                +   G IS++P++VD+V    IP+IAAGGI D RG +A++ LGA GV +GT F+ 
Sbjct: 190 KPKNQLPMVGTISLVPQIVDVV---SIPVIAAGGIMDGRGVLASIVLGAEGVQMGTAFLT 249

Query: 246 TEESHAHPAYKRKLVELDATDYTNIFGRARWPGAPQRVLQTPFYDDWKSLPANENESNQP 305
           +++S+A    +  ++    TD   +  +A + G   R +   F ++      ++ E + P
Sbjct: 250 SQDSNASELLRDAIINSKETD--TVITKA-FSGKLARGINNRFIEE-----MSQYEGDIP 309

Query: 306 VIGRSTINGLDIDVRRFAGTVPNATTKGDIESMAMYAGQGVGLIKEILPAGEVVRKLVEG 327
                  N L   +R+ A  +      GD E + M++GQ   L     PA  ++  ++  
Sbjct: 310 --DYPIQNELTSSIRKAAANI------GDKELIHMWSGQSPRL-ATTHPANTIMSNIINQ 353

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BLAST of Cp4.1LG15g03680 vs. TrEMBL
Match: A0A0A0K422_CUCSA (Uncharacterized protein OS=Cucumis sativus GN=Csa_7G064010 PE=4 SV=1)

HSP 1 Score: 640.6 bits (1651), Expect = 1.1e-180
Identity = 317/336 (94.35%), Postives = 330/336 (98.21%), Query Frame = 1


Query: 1   MDSGGILGFQYGIVQAPLGPDISGPELVAAVANAGGLGLLRSPDWESPDYVRELIRKTRA 60
           M  GG+LGFQYGIVQAPLGPDISGPELVAAVANAGGLGLLR+PDWESPDYVRELIRKTRA
Sbjct: 55  MGFGGLLGFQYGIVQAPLGPDISGPELVAAVANAGGLGLLRAPDWESPDYVRELIRKTRA 114

Query: 61  LTDKPFGVGVILAFPHEQNLKAILDEKVAVLQVYWGECSKALVDQAHSAGVKIIPQVGSV 120
           LTDKPFG+GVILAFPHEQNLKAILDEKVAV+QVYWGECSK LVDQ HSAGVKIIPQVGSV
Sbjct: 115 LTDKPFGIGVILAFPHEQNLKAILDEKVAVVQVYWGECSKDLVDQVHSAGVKIIPQVGSV 174

Query: 121 EEASKAVDVGVDAIIVQGREAGGHVIGQEGLISILPRVVDIVGNEDIPIIAAGGISDSRG 180
           EEASKAVDVGVDAIIVQGREAGGHVIGQEGLISILPRVV+IVG++DIP+IAAG ISDSRG
Sbjct: 175 EEASKAVDVGVDAIIVQGREAGGHVIGQEGLISILPRVVEIVGDKDIPVIAAGSISDSRG 234

Query: 181 YVAALALGARGVCLGTRFVATEESHAHPAYKRKLVELDATDYTNIFGRARWPGAPQRVLQ 240
           YVAALALGARGVCLGTRFVATEES+AHP YKRKLVEL+ATDYTNIFGRARWPGAPQRVLQ
Sbjct: 235 YVAALALGARGVCLGTRFVATEESNAHPTYKRKLVELEATDYTNIFGRARWPGAPQRVLQ 294

Query: 241 TPFYDDWKSLPANENESNQPVIGRSTINGLDIDVRRFAGTVPNATTKGDIESMAMYAGQG 300
           TPFYDDWKSLPANENESNQPVIGRSTINGLDID+RRFAGTVPNATTKGDIESMAMYAGQG
Sbjct: 295 TPFYDDWKSLPANENESNQPVIGRSTINGLDIDIRRFAGTVPNATTKGDIESMAMYAGQG 354

Query: 301 VGLIKEILPAGEVVRKLVEGAQHLIQTHLSSFVHKD 337
           VGLIKEILPAGEVVR+LVEGAQHLI+THLSSFVHKD
Sbjct: 355 VGLIKEILPAGEVVRRLVEGAQHLIRTHLSSFVHKD 390

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BLAST of Cp4.1LG15g03680 vs. TrEMBL
Match: B9IJE3_POPTR (Uncharacterized protein OS=Populus trichocarpa GN=POPTR_0017s07870g PE=4 SV=1)

HSP 1 Score: 548.5 bits (1412), Expect = 5.6e-153
Identity = 266/322 (82.61%), Postives = 296/322 (91.93%), Query Frame = 1


Query: 5   GILGFQYGIVQAPLGPDISGPELVAAVANAGGLGLLRSPDWESPDYVRELIRKTRALTDK 64
           GILGF+YGIVQAPLGPDISGPELVAAVANAGGLGLLR+PDWESPDY+RELIRKTR+LTDK
Sbjct: 5   GILGFEYGIVQAPLGPDISGPELVAAVANAGGLGLLRAPDWESPDYLRELIRKTRSLTDK 64

Query: 65  PFGVGVILAFPHEQNLKAILDEKVAVLQVYWGECSKALVDQAHSAGVKIIPQVGSVEEAS 124
           PFGVG++LAFPHE+NLK ILDEKVAVLQVYWGECSK LV +AH AGVK++PQVGS E+A 
Sbjct: 65  PFGVGLVLAFPHEENLKVILDEKVAVLQVYWGECSKELVLEAHLAGVKVVPQVGSFEDAR 124

Query: 125 KAVDVGVDAIIVQGREAGGHVIGQEGLISILPRVVDIVGNEDIPIIAAGGISDSRGYVAA 184
           KA+D GVD IIVQGREAGGHVIGQEGLISILPRVVD+VG+ DIP+IAAGGI ++RGYVAA
Sbjct: 125 KAIDAGVDGIIVQGREAGGHVIGQEGLISILPRVVDLVGDRDIPVIAAGGIVNARGYVAA 184

Query: 185 LALGARGVCLGTRFVATEESHAHPAYKRKLVELDATDYTNIFGRARWPGAPQRVLQTPFY 244
           LALGA+GVCLGTRFVATEES+AHP YKRKLVELD T+YTNIFGRARWP APQR L TPF+
Sbjct: 185 LALGAKGVCLGTRFVATEESNAHPTYKRKLVELDRTEYTNIFGRARWPNAPQRALVTPFF 244

Query: 245 DDWKSLPANENESNQPVIGRSTINGLDIDVRRFAGTVPNATTKGDIESMAMYAGQGVGLI 304
           +DWKSLP +ENE NQPVIGRSTING + ++RRF G VPN TT GDIESMAMYAGQGVGLI
Sbjct: 245 NDWKSLPPHENEINQPVIGRSTINGEEKEIRRFGGPVPNMTTTGDIESMAMYAGQGVGLI 304

Query: 305 KEILPAGEVVRKLVEGAQHLIQ 327
           KEI PA EV+++LVEGAQ LI+
Sbjct: 305 KEIWPASEVIKRLVEGAQQLIR 326

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BLAST of Cp4.1LG15g03680 vs. TrEMBL
Match: A0A067L9X1_JATCU (Uncharacterized protein OS=Jatropha curcas GN=JCGZ_24913 PE=4 SV=1)

HSP 1 Score: 540.0 bits (1390), Expect = 2.0e-150
Identity = 263/327 (80.43%), Postives = 295/327 (90.21%), Query Frame = 1


Query: 5   GILGFQYGIVQAPLGPDISGPELVAAVANAGGLGLLRSPDWESPDYVRELIRKTRALTDK 64
           GILGF+YGIVQAPLGPDISGPELVAAVANAGGLG LR+PDWESPDYVR LIRKTR LTDK
Sbjct: 5   GILGFEYGIVQAPLGPDISGPELVAAVANAGGLGFLRAPDWESPDYVRGLIRKTRTLTDK 64

Query: 65  PFGVGVILAFPHEQNLKAILDEKVAVLQVYWGECSKALVDQAHSAGVKIIPQVGSVEEAS 124
           PFG+GV+LAFPHE+N+KAILDEKVAVLQVYWG+CS+ LV +AH AGVK++PQVGS E A 
Sbjct: 65  PFGIGVVLAFPHEENVKAILDEKVAVLQVYWGDCSEELVLKAHQAGVKVVPQVGSYEGAE 124

Query: 125 KAVDVGVDAIIVQGREAGGHVIGQEGLISILPRVVDIVGNEDIPIIAAGGISDSRGYVAA 184
           KA+ VGVDAIIVQGREAGGHVIGQEGLIS+LPRVVD+VG   IP+IAAGGI D+RGYVAA
Sbjct: 125 KAISVGVDAIIVQGREAGGHVIGQEGLISLLPRVVDLVGVRGIPVIAAGGIVDARGYVAA 184

Query: 185 LALGARGVCLGTRFVATEESHAHPAYKRKLVELDATDYTNIFGRARWPGAPQRVLQTPFY 244
           LALGA+GVCLGTRF+A EES+AHP YKRKL+EL  T++TN+FGRARWP APQRVL TPF+
Sbjct: 185 LALGAQGVCLGTRFLAAEESYAHPIYKRKLIELSKTEHTNVFGRARWPNAPQRVLVTPFF 244

Query: 245 DDWKSLPANENESNQPVIGRSTINGLDIDVRRFAGTVPNATTKGDIESMAMYAGQGVGLI 304
           +DWKSLP++E+E NQPVIGRSTING + ++RRF GTVPN TT GDIESMAMYAGQGVGLI
Sbjct: 245 NDWKSLPSHESEINQPVIGRSTINGEEKEIRRFGGTVPNRTTTGDIESMAMYAGQGVGLI 304

Query: 305 KEILPAGEVVRKLVEGAQHLIQTHLSS 332
            EILPA EVV+ LVEGAQ LIQ  LSS
Sbjct: 305 DEILPASEVVKMLVEGAQQLIQQELSS 331

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BLAST of Cp4.1LG15g03680 vs. TrEMBL
Match: A0A061E2B3_THECC (Aldolase-type TIM barrel family protein isoform 1 OS=Theobroma cacao GN=TCM_007438 PE=4 SV=1)

HSP 1 Score: 538.1 bits (1385), Expect = 7.6e-150
Identity = 256/326 (78.53%), Postives = 301/326 (92.33%), Query Frame = 1


Query: 5   GILGFQYGIVQAPLGPDISGPELVAAVANAGGLGLLRSPDWESPDYVRELIRKTRALTDK 64
           GILGF+YGIVQAPLGPDI+GPELVAAVANAGGLGLLR+PDWESP+Y++ELIRKTR LTDK
Sbjct: 5   GILGFEYGIVQAPLGPDIAGPELVAAVANAGGLGLLRAPDWESPEYLKELIRKTRMLTDK 64

Query: 65  PFGVGVILAFPHEQNLKAILDEKVAVLQVYWGECSKALVDQAHSAGVKIIPQVGSVEEAS 124
           PFGVGV+LAFPH++N+KAIL+EKVAVLQ+YWGECSK LV +AH+AGVK++PQVGSVEEA 
Sbjct: 65  PFGVGVVLAFPHKENIKAILEEKVAVLQLYWGECSKELVLEAHNAGVKVVPQVGSVEEAK 124

Query: 125 KAVDVGVDAIIVQGREAGGHVIGQEGLISILPRVVDIVGNEDIPIIAAGGISDSRGYVAA 184
           KA++VGVDAIIVQGREAGGHVIGQEGLIS+LP+VVD+VG+ DIP+IAAG I D+RGYVAA
Sbjct: 125 KALNVGVDAIIVQGREAGGHVIGQEGLISLLPQVVDLVGDRDIPVIAAGSIVDARGYVAA 184

Query: 185 LALGARGVCLGTRFVATEESHAHPAYKRKLVELDATDYTNIFGRARWPGAPQRVLQTPFY 244
           L LGA+G+C+GTRF+AT ES+AHP YKRKL+E D T+YT++FGRARWPGAP RVLQTPF+
Sbjct: 185 LTLGAKGICMGTRFLATHESYAHPTYKRKLIEHDKTEYTDVFGRARWPGAPHRVLQTPFF 244

Query: 245 DDWKSLPANENESNQPVIGRSTINGLDIDVRRFAGTVPNATTKGDIESMAMYAGQGVGLI 304
            DWKSLP +ENE+ QP+IGRS I+G++ ++RRFAGTVPN TT GDIESMAMYAGQ VGLI
Sbjct: 245 CDWKSLPVHENETTQPIIGRSIIHGVEKEIRRFAGTVPNPTTTGDIESMAMYAGQSVGLI 304

Query: 305 KEILPAGEVVRKLVEGAQHLIQTHLS 331
           KEI+PAGEVV++LVEGAQ  IQ  LS
Sbjct: 305 KEIVPAGEVVKRLVEGAQLAIQQILS 330

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BLAST of Cp4.1LG15g03680 vs. TrEMBL
Match: D7TU94_VITVI (Putative uncharacterized protein OS=Vitis vinifera GN=VIT_03s0017g01240 PE=4 SV=1)

HSP 1 Score: 536.2 bits (1380), Expect = 2.9e-149
Identity = 256/332 (77.11%), Postives = 295/332 (88.86%), Query Frame = 1


Query: 5   GILGFQYGIVQAPLGPDISGPELVAAVANAGGLGLLRSPDWESPDYVRELIRKTRALTDK 64
           G+LGF+YG+VQAPLGPDISGPELVAAVANAG +G LR+PDWE+PDY+R LI+KTR LTDK
Sbjct: 5   GVLGFEYGVVQAPLGPDISGPELVAAVANAGAIGFLRAPDWETPDYLRGLIKKTRTLTDK 64

Query: 65  PFGVGVILAFPHEQNLKAILDEKVAVLQVYWGECSKALVDQAHSAGVKIIPQVGSVEEAS 124
           PFGV VILAFPHE+N+KAIL EKVAVLQV WG+  + LV +AH AGVK++PQVGS EEA 
Sbjct: 65  PFGVAVILAFPHEENMKAILAEKVAVLQVSWGDVREELVHEAHCAGVKVVPQVGSFEEAR 124

Query: 125 KAVDVGVDAIIVQGREAGGHVIGQEGLISILPRVVDIVGNEDIPIIAAGGISDSRGYVAA 184
           KA+DVGVDAIIVQGREAGGHVIGQ+GLIS+LPRVVD+VG+ DIP+IAAGGI D RGYVAA
Sbjct: 125 KAIDVGVDAIIVQGREAGGHVIGQDGLISLLPRVVDLVGDRDIPVIAAGGIVDERGYVAA 184

Query: 185 LALGARGVCLGTRFVATEESHAHPAYKRKLVELDATDYTNIFGRARWPGAPQRVLQTPFY 244
           LALGA+G+CLGTRFVATEESHAHP YKRKL+ELD T+YTN+FGRARWPGAPQRVL+TPF+
Sbjct: 185 LALGAQGICLGTRFVATEESHAHPIYKRKLLELDETEYTNVFGRARWPGAPQRVLETPFF 244

Query: 245 DDWKSLPANENESNQPVIGRSTINGLDIDVRRFAGTVPNATTKGDIESMAMYAGQGVGLI 304
            DWKSLPA+E+E NQP+IG STING++ D+RRF+GTVPN TT GDIESM +YAG+GVGLI
Sbjct: 245 KDWKSLPAHESEINQPLIGHSTINGMEKDIRRFSGTVPNRTTTGDIESMVLYAGEGVGLI 304

Query: 305 KEILPAGEVVRKLVEGAQHLIQTHLSSFVHKD 337
           KEILPA EVV++LVEGAQ LI         KD
Sbjct: 305 KEILPAAEVVKRLVEGAQLLIHQQFGGLKSKD 336

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BLAST of Cp4.1LG15g03680 vs. TAIR10
Match: AT5G64250.2 (AT5G64250.2 Aldolase-type TIM barrel family protein)

HSP 1 Score: 533.1 bits (1372), Expect = 1.2e-151
Identity = 253/321 (78.82%), Postives = 294/321 (91.59%), Query Frame = 1


Query: 5   GILGFQYGIVQAPLGPDISGPELVAAVANAGGLGLLRSPDWESPDYVRELIRKTRALTDK 64
           GILGF+YGIVQAPLGPDISGPELVAAVANAGG+GLLR PDWE PDY+RELIRKT+ LT+K
Sbjct: 3   GILGFEYGIVQAPLGPDISGPELVAAVANAGGIGLLRCPDWECPDYLRELIRKTKTLTEK 62

Query: 65  PFGVGVILAFPHEQNLKAILDEKVAVLQVYWGECSKALVDQAHSAGVKIIPQVGSVEEAS 124
           PFG+GV+LAFPH+ N+K IL+EKVAVLQ+YWG+CS+ LVD AH AGVK+IPQVG+VEEA 
Sbjct: 63  PFGIGVVLAFPHDLNIKTILEEKVAVLQLYWGDCSQELVDDAHFAGVKVIPQVGNVEEAR 122

Query: 125 KAVDVGVDAIIVQGREAGGHVIGQEGLISILPRVVDIVGNEDIPIIAAGGISDSRGYVAA 184
           KAV VGVDAIIVQGREAGGHVIG++GL S+LPRVVD+VG  DIP+IAAGGI D RGYVAA
Sbjct: 123 KAVAVGVDAIIVQGREAGGHVIGKDGLFSLLPRVVDLVGERDIPVIAAGGIVDGRGYVAA 182

Query: 185 LALGARGVCLGTRFVATEESHAHPAYKRKLVELDATDYTNIFGRARWPGAPQRVLQTPFY 244
           L+LGA+GVCLGTRFVAT ES+AHP YKRKL+E + T+YT++FGRARWPGAPQRVL+TPF+
Sbjct: 183 LSLGAQGVCLGTRFVATHESYAHPIYKRKLIEYEKTEYTDVFGRARWPGAPQRVLETPFF 242

Query: 245 DDWKSLPANENESNQPVIGRSTINGLDIDVRRFAGTVPNATTKGDIESMAMYAGQGVGLI 304
           DDW+SLPA+ENE NQP+IGRSTI+G++ ++RRF+GTVPN TT GD+ESMAMYAGQ VGLI
Sbjct: 243 DDWRSLPAHENEVNQPIIGRSTIHGVEKEIRRFSGTVPNMTTTGDLESMAMYAGQSVGLI 302

Query: 305 KEILPAGEVVRKLVEGAQHLI 326
           KEILPAGEVV+ LVE AQ LI
Sbjct: 303 KEILPAGEVVKSLVEEAQALI 323

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BLAST of Cp4.1LG15g03680 vs. NCBI nr
Match: gi|659110334|ref|XP_008455173.1| (PREDICTED: nitronate monooxygenase isoform X1 [Cucumis melo])

HSP 1 Score: 646.4 bits (1666), Expect = 2.8e-182
Identity = 319/336 (94.94%), Postives = 329/336 (97.92%), Query Frame = 1


Query: 1   MDSGGILGFQYGIVQAPLGPDISGPELVAAVANAGGLGLLRSPDWESPDYVRELIRKTRA 60
           M  GG+LGFQYGIVQAPLGPDISGPELVAAVANAGGLGLLR+PDWESPDYVRELIRKTRA
Sbjct: 1   MSFGGLLGFQYGIVQAPLGPDISGPELVAAVANAGGLGLLRAPDWESPDYVRELIRKTRA 60

Query: 61  LTDKPFGVGVILAFPHEQNLKAILDEKVAVLQVYWGECSKALVDQAHSAGVKIIPQVGSV 120
           LTDKPFG+GVILAFPHEQNLKAILDEKVAV+QVYWGECSK LVDQ HSAGVKIIPQVGSV
Sbjct: 61  LTDKPFGIGVILAFPHEQNLKAILDEKVAVVQVYWGECSKDLVDQVHSAGVKIIPQVGSV 120

Query: 121 EEASKAVDVGVDAIIVQGREAGGHVIGQEGLISILPRVVDIVGNEDIPIIAAGGISDSRG 180
           EEASKAVD GVDAIIVQGREAGGHVIGQEGLISILPRVV+IVGNEDIP+IAAG ISDSRG
Sbjct: 121 EEASKAVDAGVDAIIVQGREAGGHVIGQEGLISILPRVVEIVGNEDIPVIAAGSISDSRG 180

Query: 181 YVAALALGARGVCLGTRFVATEESHAHPAYKRKLVELDATDYTNIFGRARWPGAPQRVLQ 240
           YVAALALGARGVCLGTRFVATEESHAHP YKRKLVE++ATDYTNIFGRARWPGAPQRVLQ
Sbjct: 181 YVAALALGARGVCLGTRFVATEESHAHPTYKRKLVEMEATDYTNIFGRARWPGAPQRVLQ 240

Query: 241 TPFYDDWKSLPANENESNQPVIGRSTINGLDIDVRRFAGTVPNATTKGDIESMAMYAGQG 300
           TPFYDDWKSLPANENESNQPVIGRSTINGLDID+RRFAGTVPNATTKGDIESMAMYAGQG
Sbjct: 241 TPFYDDWKSLPANENESNQPVIGRSTINGLDIDIRRFAGTVPNATTKGDIESMAMYAGQG 300

Query: 301 VGLIKEILPAGEVVRKLVEGAQHLIQTHLSSFVHKD 337
           VGLIKEILPAGEVVR+LVEGAQHLIQTHLSSFVHKD
Sbjct: 301 VGLIKEILPAGEVVRRLVEGAQHLIQTHLSSFVHKD 336

[Close]

BLAST of Cp4.1LG15g03680 vs. NCBI nr
Match: gi|778724449|ref|XP_011658807.1| (PREDICTED: nitronate monooxygenase isoform X1 [Cucumis sativus])

HSP 1 Score: 640.6 bits (1651), Expect = 1.6e-180
Identity = 317/336 (94.35%), Postives = 330/336 (98.21%), Query Frame = 1


Query: 1   MDSGGILGFQYGIVQAPLGPDISGPELVAAVANAGGLGLLRSPDWESPDYVRELIRKTRA 60
           M  GG+LGFQYGIVQAPLGPDISGPELVAAVANAGGLGLLR+PDWESPDYVRELIRKTRA
Sbjct: 55  MGFGGLLGFQYGIVQAPLGPDISGPELVAAVANAGGLGLLRAPDWESPDYVRELIRKTRA 114

Query: 61  LTDKPFGVGVILAFPHEQNLKAILDEKVAVLQVYWGECSKALVDQAHSAGVKIIPQVGSV 120
           LTDKPFG+GVILAFPHEQNLKAILDEKVAV+QVYWGECSK LVDQ HSAGVKIIPQVGSV
Sbjct: 115 LTDKPFGIGVILAFPHEQNLKAILDEKVAVVQVYWGECSKDLVDQVHSAGVKIIPQVGSV 174

Query: 121 EEASKAVDVGVDAIIVQGREAGGHVIGQEGLISILPRVVDIVGNEDIPIIAAGGISDSRG 180
           EEASKAVDVGVDAIIVQGREAGGHVIGQEGLISILPRVV+IVG++DIP+IAAG ISDSRG
Sbjct: 175 EEASKAVDVGVDAIIVQGREAGGHVIGQEGLISILPRVVEIVGDKDIPVIAAGSISDSRG 234

Query: 181 YVAALALGARGVCLGTRFVATEESHAHPAYKRKLVELDATDYTNIFGRARWPGAPQRVLQ 240
           YVAALALGARGVCLGTRFVATEES+AHP YKRKLVEL+ATDYTNIFGRARWPGAPQRVLQ
Sbjct: 235 YVAALALGARGVCLGTRFVATEESNAHPTYKRKLVELEATDYTNIFGRARWPGAPQRVLQ 294

Query: 241 TPFYDDWKSLPANENESNQPVIGRSTINGLDIDVRRFAGTVPNATTKGDIESMAMYAGQG 300
           TPFYDDWKSLPANENESNQPVIGRSTINGLDID+RRFAGTVPNATTKGDIESMAMYAGQG
Sbjct: 295 TPFYDDWKSLPANENESNQPVIGRSTINGLDIDIRRFAGTVPNATTKGDIESMAMYAGQG 354

Query: 301 VGLIKEILPAGEVVRKLVEGAQHLIQTHLSSFVHKD 337
           VGLIKEILPAGEVVR+LVEGAQHLI+THLSSFVHKD
Sbjct: 355 VGLIKEILPAGEVVRRLVEGAQHLIRTHLSSFVHKD 390

[Close]

BLAST of Cp4.1LG15g03680 vs. NCBI nr
Match: gi|224140777|ref|XP_002323755.1| (hypothetical protein POPTR_0017s07870g [Populus trichocarpa])

HSP 1 Score: 548.5 bits (1412), Expect = 8.1e-153
Identity = 266/322 (82.61%), Postives = 296/322 (91.93%), Query Frame = 1


Query: 5   GILGFQYGIVQAPLGPDISGPELVAAVANAGGLGLLRSPDWESPDYVRELIRKTRALTDK 64
           GILGF+YGIVQAPLGPDISGPELVAAVANAGGLGLLR+PDWESPDY+RELIRKTR+LTDK
Sbjct: 5   GILGFEYGIVQAPLGPDISGPELVAAVANAGGLGLLRAPDWESPDYLRELIRKTRSLTDK 64

Query: 65  PFGVGVILAFPHEQNLKAILDEKVAVLQVYWGECSKALVDQAHSAGVKIIPQVGSVEEAS 124
           PFGVG++LAFPHE+NLK ILDEKVAVLQVYWGECSK LV +AH AGVK++PQVGS E+A 
Sbjct: 65  PFGVGLVLAFPHEENLKVILDEKVAVLQVYWGECSKELVLEAHLAGVKVVPQVGSFEDAR 124

Query: 125 KAVDVGVDAIIVQGREAGGHVIGQEGLISILPRVVDIVGNEDIPIIAAGGISDSRGYVAA 184
           KA+D GVD IIVQGREAGGHVIGQEGLISILPRVVD+VG+ DIP+IAAGGI ++RGYVAA
Sbjct: 125 KAIDAGVDGIIVQGREAGGHVIGQEGLISILPRVVDLVGDRDIPVIAAGGIVNARGYVAA 184

Query: 185 LALGARGVCLGTRFVATEESHAHPAYKRKLVELDATDYTNIFGRARWPGAPQRVLQTPFY 244
           LALGA+GVCLGTRFVATEES+AHP YKRKLVELD T+YTNIFGRARWP APQR L TPF+
Sbjct: 185 LALGAKGVCLGTRFVATEESNAHPTYKRKLVELDRTEYTNIFGRARWPNAPQRALVTPFF 244

Query: 245 DDWKSLPANENESNQPVIGRSTINGLDIDVRRFAGTVPNATTKGDIESMAMYAGQGVGLI 304
           +DWKSLP +ENE NQPVIGRSTING + ++RRF G VPN TT GDIESMAMYAGQGVGLI
Sbjct: 245 NDWKSLPPHENEINQPVIGRSTINGEEKEIRRFGGPVPNMTTTGDIESMAMYAGQGVGLI 304

Query: 305 KEILPAGEVVRKLVEGAQHLIQ 327
           KEI PA EV+++LVEGAQ LI+
Sbjct: 305 KEIWPASEVIKRLVEGAQQLIR 326

[Close]

BLAST of Cp4.1LG15g03680 vs. NCBI nr
Match: gi|1012250541|ref|XP_015943467.1| (PREDICTED: nitronate monooxygenase [Arachis duranensis])

HSP 1 Score: 547.0 bits (1408), Expect = 2.3e-152
Identity = 259/326 (79.45%), Postives = 301/326 (92.33%), Query Frame = 1


Query: 5   GILGFQYGIVQAPLGPDISGPELVAAVANAGGLGLLRSPDWESPDYVRELIRKTRALTDK 64
           G+LGF+YGIVQAPLGPDISGPELVAAVANAGGLGLLR+PDWESPDYVR LI KTR+LTDK
Sbjct: 5   GLLGFEYGIVQAPLGPDISGPELVAAVANAGGLGLLRAPDWESPDYVRGLINKTRSLTDK 64

Query: 65  PFGVGVILAFPHEQNLKAILDEKVAVLQVYWGECSKALVDQAHSAGVKIIPQVGSVEEAS 124
           PFG+GV+LAFPHEQNLKAILDEKVA+LQVYWG+C+  LV  AHSAGVKI+PQVG +  A 
Sbjct: 65  PFGIGVVLAFPHEQNLKAILDEKVAILQVYWGDCTPDLVAMAHSAGVKIVPQVGDLRGAK 124

Query: 125 KAVDVGVDAIIVQGREAGGHVIGQEGLISILPRVVDIVGNEDIPIIAAGGISDSRGYVAA 184
           +A++ GVD IIVQGREAGGHVIGQ+GLIS++P VVD+VGN +IP+IAAGGI D+RGYVAA
Sbjct: 125 QAINAGVDGIIVQGREAGGHVIGQDGLISLVPTVVDLVGNRNIPVIAAGGIVDARGYVAA 184

Query: 185 LALGARGVCLGTRFVATEESHAHPAYKRKLVELDATDYTNIFGRARWPGAPQRVLQTPFY 244
           LALGA+GVCLGTRFVATEES+AHP YKRKL+E+D T+YT++FGRARWPGAPQRVL+TPFY
Sbjct: 185 LALGAQGVCLGTRFVATEESYAHPTYKRKLIEMDETEYTDVFGRARWPGAPQRVLKTPFY 244

Query: 245 DDWKSLPANENESNQPVIGRSTINGLDIDVRRFAGTVPNATTKGDIESMAMYAGQGVGLI 304
            DW+SLP++ENE+NQP+IG STI+G+D ++RRFAGTVPN TT GDIESMAMYAGQGVGLI
Sbjct: 245 KDWRSLPSHENETNQPLIGHSTIHGMDREIRRFAGTVPNMTTTGDIESMAMYAGQGVGLI 304

Query: 305 KEILPAGEVVRKLVEGAQHLIQTHLS 331
           KEILPAGEVV++LVEGAQ LI+ H +
Sbjct: 305 KEILPAGEVVKRLVEGAQILIKQHFN 330

[Close]

BLAST of Cp4.1LG15g03680 vs. NCBI nr
Match: gi|743899655|ref|XP_011043115.1| (PREDICTED: nitronate monooxygenase [Populus euphratica])

HSP 1 Score: 545.8 bits (1405), Expect = 5.2e-152
Identity = 265/322 (82.30%), Postives = 295/322 (91.61%), Query Frame = 1


Query: 5   GILGFQYGIVQAPLGPDISGPELVAAVANAGGLGLLRSPDWESPDYVRELIRKTRALTDK 64
           GILGF+YGIVQAPLGPDISGPELVAAVANAGGLGLLR+PDWESPDY+RELIRKTR+LTDK
Sbjct: 5   GILGFEYGIVQAPLGPDISGPELVAAVANAGGLGLLRAPDWESPDYLRELIRKTRSLTDK 64

Query: 65  PFGVGVILAFPHEQNLKAILDEKVAVLQVYWGECSKALVDQAHSAGVKIIPQVGSVEEAS 124
           PFGVG++LAFPHE+NLK ILDEKVAVLQVYWGECSK LV +AH AGVK++PQVGS E+A 
Sbjct: 65  PFGVGLVLAFPHEENLKVILDEKVAVLQVYWGECSKELVLEAHLAGVKVVPQVGSFEDAR 124

Query: 125 KAVDVGVDAIIVQGREAGGHVIGQEGLISILPRVVDIVGNEDIPIIAAGGISDSRGYVAA 184
           KA+D GVD IIVQGREAGGHVIGQEGLISILPRVVD+VG+ DIP+IAAGGI ++RGYVAA
Sbjct: 125 KAIDAGVDGIIVQGREAGGHVIGQEGLISILPRVVDLVGDRDIPVIAAGGIVNARGYVAA 184

Query: 185 LALGARGVCLGTRFVATEESHAHPAYKRKLVELDATDYTNIFGRARWPGAPQRVLQTPFY 244
           LALGA+GVCLGTRFVATEES+AHP YKRKLVELD T YTNIFGRARWP APQR L TPF+
Sbjct: 185 LALGAKGVCLGTRFVATEESNAHPTYKRKLVELDRTQYTNIFGRARWPNAPQRALVTPFF 244

Query: 245 DDWKSLPANENESNQPVIGRSTINGLDIDVRRFAGTVPNATTKGDIESMAMYAGQGVGLI 304
           +DWKSLP +ENE NQPVIGRSTING + ++ RF G+VPN TT GDIESMAMYAGQGVGLI
Sbjct: 245 NDWKSLPPHENEINQPVIGRSTINGEEKEIWRFGGSVPNVTTTGDIESMAMYAGQGVGLI 304

Query: 305 KEILPAGEVVRKLVEGAQHLIQ 327
           KEI PA EV+++LVEGAQ LI+
Sbjct: 305 KEIWPASEVIKRLVEGAQQLIR 326

The following BLAST results are available for this feature:

BLAST of Cp4.1LG15g03680 vs. Swiss-Prot


Match Name	E-value	Identity	Description
2NPD_PSEAE	1.5e-26	30.79	Nitronate monooxygenase OS=Pseudomonas aeruginosa (strain ATCC 15692 / PAO1 / 1C...	[more]
2NPD_NEUCR	2.6e-26	28.57	Nitronate monooxygenase OS=Neurospora crassa (strain ATCC 24698 / 74-OR23-1A / C...	[more]
2NPD_STAS1	3.8e-25	26.83	Probable nitronate monooxygenase OS=Staphylococcus saprophyticus subsp. saprophy...	[more]
2NPD_STAA3	6.4e-25	27.60	Probable nitronate monooxygenase OS=Staphylococcus aureus (strain USA300) GN=SAU...	[more]
2NPD_STAA8	6.4e-25	27.60	Probable nitronate monooxygenase OS=Staphylococcus aureus (strain NCTC 8325) GN=...	[more]

BLAST of Cp4.1LG15g03680 vs. TrEMBL


Match Name	E-value	Identity	Description
A0A0A0K422_CUCSA	1.1e-180	94.35	Uncharacterized protein OS=Cucumis sativus GN=Csa_7G064010 PE=4 SV=1	[more]
B9IJE3_POPTR	5.6e-153	82.61	Uncharacterized protein OS=Populus trichocarpa GN=POPTR_0017s07870g PE=4 SV=1	[more]
A0A067L9X1_JATCU	2.0e-150	80.43	Uncharacterized protein OS=Jatropha curcas GN=JCGZ_24913 PE=4 SV=1	[more]
A0A061E2B3_THECC	7.6e-150	78.53	Aldolase-type TIM barrel family protein isoform 1 OS=Theobroma cacao GN=TCM_0074...	[more]
D7TU94_VITVI	2.9e-149	77.11	Putative uncharacterized protein OS=Vitis vinifera GN=VIT_03s0017g01240 PE=4 SV=...	[more]

BLAST of Cp4.1LG15g03680 vs. TAIR10


Match Name	E-value	Identity	Description
AT5G64250.2	1.2e-151	78.82	Aldolase-type TIM barrel family protein	[more]

BLAST of Cp4.1LG15g03680 vs. NCBI nr


Match Name	E-value	Identity	Description
gi\|659110334\|ref\|XP_008455173.1\|	2.8e-182	94.94	PREDICTED: nitronate monooxygenase isoform X1 [Cucumis melo]	[more]
gi\|778724449\|ref\|XP_011658807.1\|	1.6e-180	94.35	PREDICTED: nitronate monooxygenase isoform X1 [Cucumis sativus]	[more]
gi\|224140777\|ref\|XP_002323755.1\|	8.1e-153	82.61	hypothetical protein POPTR_0017s07870g [Populus trichocarpa]	[more]
gi\|1012250541\|ref\|XP_015943467.1\|	2.3e-152	79.45	PREDICTED: nitronate monooxygenase [Arachis duranensis]	[more]
gi\|743899655\|ref\|XP_011043115.1\|	5.2e-152	82.30	PREDICTED: nitronate monooxygenase [Populus euphratica]	[more]

Annotated Terms

The following terms have been associated with this gene:

Vocabulary: **Molecular Function**
Term	Definition
GO:0003824	catalytic activity
GO:0018580	nitronate monooxygenase activity

Vocabulary: **Biological Process**
Term	Definition
GO:0055114	oxidation-reduction process

Vocabulary: **INTERPRO**
Term	Definition
IPR013785	Aldolase_TIM
IPR004136	NMO

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0055114	oxidation-reduction process
biological_process	GO:0046482	para-aminobenzoic acid metabolic process
biological_process	GO:0046686	response to cadmium ion
biological_process	GO:0009610	response to symbiotic fungus
biological_process	GO:0006807	nitrogen compound metabolic process
cellular_component	GO:0005829	cytosol
cellular_component	GO:0005575	cellular_component
molecular_function	GO:0051213	dioxygenase activity
molecular_function	GO:0018580	nitronate monooxygenase activity
molecular_function	GO:0003824	catalytic activity

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Cp4.1LG15g03680.1	Cp4.1LG15g03680.1	mRNA

InterPro

Analysis Name: InterPro Annotations of Cucurbita pepo
Date Performed: 2017-12-02


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR004136	Nitronate monooxygenase	PFAM	PF03060	NMO	coord: 76..319 score: 1.7E-45coord: 6..75 score: 3.6
IPR013785	Aldolase-type TIM barrel	GENE3D	G3DSA:3.20.20.70		coord: 5..315 score: 4.7
None	No IPR available	PANTHER	PTHR32332	FAMILY NOT NAMED	coord: 6..336 score: 1.1E
None	No IPR available	PANTHER	PTHR32332:SF20	2-NITROPROPANE DIOXYGENASE-LIKE PROTEIN	coord: 6..336 score: 1.1E
None	No IPR available	unknown	SSF51412	Inosine monophosphate dehydrogenase (IMPDH)	coord: 4..326 score: 8.7

Synteny

The following gene(s) are orthologous to this gene:


Gene	Orthologue	Organism	Block
Cp4.1LG15g03680	Cucsa.218110	Cucumber (Gy14) v1	cgycpeB0594
Cp4.1LG15g03680	CmaCh11G016550	Cucurbita maxima (Rimu)	cmacpeB116
Cp4.1LG15g03680	Cla012877	Watermelon (97103) v1	cpewmB237
Cp4.1LG15g03680	Csa7G064010	Cucumber (Chinese Long) v2	cpecuB258
Cp4.1LG15g03680	ClCG09G015620	Watermelon (Charleston Gray)	cpewcgB218
Cp4.1LG15g03680	CSPI07G05730	Wild cucumber (PI 183967)	cpecpiB258
Cp4.1LG15g03680	Lsi03G007410	Bottle gourd (USVL1VR-Ls)	cpelsiB208
Cp4.1LG15g03680	CsaV3_7G006720	Cucumber (Chinese Long) v3	cpecucB0323
Cp4.1LG15g03680	Bhi05G000730	Wax gourd	cpewgoB0317
Cp4.1LG15g03680	CsGy7G005450	Cucumber (Gy14) v2	cgybcpeB911
Cp4.1LG15g03680	Carg22823	Silver-seed gourd	carcpeB0401

The following gene(s) are paralogous to this gene:


Gene	Paralogue	Organism	Block
Cp4.1LG15g03680	Cp4.1LG04g07090	Cucurbita pepo (Zucchini)	cpecpeB269

The following block(s) are covering this gene:


Gene	Organism	Block
Cp4.1LG15g03680	Cucurbita pepo (Zucchini)	cpecpeB279
Cp4.1LG15g03680	Cucurbita maxima (Rimu)	cmacpeB522
Cp4.1LG15g03680	Cucurbita moschata (Rifu)	cmocpeB481
Cp4.1LG15g03680	Cucumber (Gy14) v2	cgybcpeB337
Cp4.1LG15g03680	Melon (DHL92) v3.6.1	cpemedB267
Cp4.1LG15g03680	Silver-seed gourd	carcpeB1091
Cp4.1LG15g03680	Cucumber (Chinese Long) v3	cpecucB0303