Lsi03G006220 (gene) Bottle gourd (USVL1VR-Ls) | Cucurbit Genomics Database (CuGenDB)

Overview


Name	Lsi03G006220
Type	gene
Organism	Lagenaria siceraria (Bottle gourd (USVL1VR-Ls))
Description	Cysteine-rich venom protein
Location	chr03 : 7757407 .. 7757904 (-)

Genome Viewer

Gene Expression

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: CDS

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGACATTTGCCAACATTCTCTCAATCCTTTTCTTAATAGGGCTAACCCTAACCTTAGCTTCCATTGCCCCCATCTTGGCCAAAAGCTATCCTAAAAATTACATTGCTGCCCACAACACTATTCGTGTAGAGGTTGGCGTTGAGCCCCTCCATTGGAACGCCACCTTGGCAGCATATGCTCAAAATTACGCAAACACGAAGATCGCCACCGGTCAAATGGAGCACTCTAGAGGACCTTATGGGGAAAACCTAGCAGAGGGATACGAAGTGATGACAGCAGAGACGGCAGTGAGTCTTTGGGCCGACGAGAAGAAACATTACGACTACAATTCTAACATGTGTGTCAACGACTCTAGCCATTGCCTCCATTATACACAGTTGGTATGGAGGAACACCAAATCTGTTGGTTGTGCCCAAGTGAAGTGCCAAAACAATTGGGTTTTTCTAATATGCAACTATTATCCTCCTGGCAATTATATAGGCCAACGTCCATATTAA

mRNA sequence

ATGACATTTGCCAACATTCTCTCAATCCTTTTCTTAATAGGGCTAACCCTAACCTTAGCTTCCATTGCCCCCATCTTGGCCAAAAGCTATCCTAAAAATTACATTGCTGCCCACAACACTATTCGTGTAGAGGTTGGCGTTGAGCCCCTCCATTGGAACGCCACCTTGGCAGCATATGCTCAAAATTACGCAAACACGAAGATCGCCACCGGTCAAATGGAGCACTCTAGAGGACCTTATGGGGAAAACCTAGCAGAGGGATACGAAGTGATGACAGCAGAGACGGCAGTGAGTCTTTGGGCCGACGAGAAGAAACATTACGACTACAATTCTAACATGTGTGTCAACGACTCTAGCCATTGCCTCCATTATACACAGTTGGTATGGAGGAACACCAAATCTGTTGGTTGTGCCCAAGTGAAGTGCCAAAACAATTGGGTTTTTCTAATATGCAACTATTATCCTCCTGGCAATTATATAGGCCAACGTCCATATTAA

Coding sequence (CDS)

ATGACATTTGCCAACATTCTCTCAATCCTTTTCTTAATAGGGCTAACCCTAACCTTAGCTTCCATTGCCCCCATCTTGGCCAAAAGCTATCCTAAAAATTACATTGCTGCCCACAACACTATTCGTGTAGAGGTTGGCGTTGAGCCCCTCCATTGGAACGCCACCTTGGCAGCATATGCTCAAAATTACGCAAACACGAAGATCGCCACCGGTCAAATGGAGCACTCTAGAGGACCTTATGGGGAAAACCTAGCAGAGGGATACGAAGTGATGACAGCAGAGACGGCAGTGAGTCTTTGGGCCGACGAGAAGAAACATTACGACTACAATTCTAACATGTGTGTCAACGACTCTAGCCATTGCCTCCATTATACACAGTTGGTATGGAGGAACACCAAATCTGTTGGTTGTGCCCAAGTGAAGTGCCAAAACAATTGGGTTTTTCTAATATGCAACTATTATCCTCCTGGCAATTATATAGGCCAACGTCCATATTAA

Protein sequence

MTFANILSILFLIGLTLTLASIAPILAKSYPKNYIAAHNTIRVEVGVEPLHWNATLAAYAQNYANTKIATGQMEHSRGPYGENLAEGYEVMTAETAVSLWADEKKHYDYNSNMCVNDSSHCLHYTQLVWRNTKSVGCAQVKCQNNWVFLICNYYPPGNYIGQRPY

Homology

[Close]

BLAST of Lsi03G006220 vs. Swiss-Prot
Match: PRB1_TOBAC (Basic form of pathogenesis-related protein 1 OS=Nicotiana tabacum PE=3 SV=1)

HSP 1 Score: 170.6 bits (431), Expect = 1.4e-41
Identity = 76/139 (54.68%), Postives = 97/139 (69.78%), Query Frame = 1


Query: 27  AKSYPKNYIAAHNTIRVEVGVEPLHWNATLAAYAQNYANTKIATGQMEHSRGPYGENLAE 86
           A++ P++Y+  HN  R +VGV P+ W+  LAAYAQNYAN +I    M HS GPYGENLA 
Sbjct: 23  AQNSPQDYLNPHNAARRQVGVGPMTWDNRLAAYAQNYANQRIGDCGMIHSHGPYGENLAA 82

Query: 87  GYEVMTAETAVSLWADEKKHYDYNSNMCVNDSSHCLHYTQLVWRNTKSVGCAQVKCQNNW 146
            +  + A  AV +W DEK+ YDYNSN CV     C HYTQ+VWRN+  +GCA+V+  N W
Sbjct: 83  AFPQLNAAGAVKMWVDEKRFYDYNSNSCV--GGVCGHYTQVVWRNSVRLGCARVRSNNGW 142

Query: 147 VFLICNYYPPGNYIGQRPY 166
            F+ CNY PPGN+IGQRP+
Sbjct: 143 FFITCNYDPPGNFIGQRPF 159

[Close]

BLAST of Lsi03G006220 vs. Swiss-Prot
Match: PR1A_TOBAC (Pathogenesis-related protein 1A OS=Nicotiana tabacum PE=1 SV=1)

HSP 1 Score: 159.1 bits (401), Expect = 4.2e-38
Identity = 81/164 (49.39%), Postives = 106/164 (64.63%), Query Frame = 1


Query: 3   FANILSILFLIGLTLTLASIAPILAKSYPKNYIAAHNTIRVEVGVEPLHWNATLAAYAQN 62
           F+ + S L +  L L L       A++  ++Y+ AHNT R +VGVEPL W+  +AAYAQN
Sbjct: 6   FSQLPSFLLVSTLLLFLVISHSCRAQNSQQDYLDAHNTARADVGVEPLTWDDQVAAYAQN 65

Query: 63  YANTKIATGQMEHSRGPYGENLAEGY-EVMTAETAVSLWADEKKHYDYNSNMCVNDSSHC 122
           YA+   A   + HS G YGENLAEG  + MTA  AV +W DEK++YD++SN C      C
Sbjct: 66  YASQLAADCNLVHSHGQYGENLAEGSGDFMTAAKAVEMWVDEKQYYDHDSNTCA-QGQVC 125

Query: 123 LHYTQLVWRNTKSVGCAQVKCQNNWVFLICNYYPPGNYIGQRPY 166
            HYTQ+VWRN+  VGCA+V+C N    + CNY PPGNY G+ PY
Sbjct: 126 GHYTQVVWRNSVRVGCARVQCNNGGYVVSCNYDPPGNYRGESPY 168

[Close]

BLAST of Lsi03G006220 vs. Swiss-Prot
Match: PR1B_TOBAC (Pathogenesis-related protein 1B OS=Nicotiana tabacum PE=2 SV=1)

HSP 1 Score: 155.2 bits (391), Expect = 6.1e-37
Identity = 80/164 (48.78%), Postives = 104/164 (63.41%), Query Frame = 1


Query: 3   FANILSILFLIGLTLTLASIAPILAKSYPKNYIAAHNTIRVEVGVEPLHWNATLAAYAQN 62
           F+ + S   +  L L L       A++  ++Y+ AHNT R +VGVEPL W+  +AAYAQN
Sbjct: 6   FSQMPSFFLVSTLLLFLIISHSSHAQNSQQDYLDAHNTARADVGVEPLTWDNGVAAYAQN 65

Query: 63  YANTKIATGQMEHSRGPYGENLAEGY-EVMTAETAVSLWADEKKHYDYNSNMCVNDSSHC 122
           Y +   A   + HS G YGENLA+G  + MTA  AV +W DEK++YD++SN C      C
Sbjct: 66  YVSQLAADCNLVHSHGQYGENLAQGSGDFMTAAKAVEMWVDEKQYYDHDSNTCAQGQV-C 125

Query: 123 LHYTQLVWRNTKSVGCAQVKCQNNWVFLICNYYPPGNYIGQRPY 166
            HYTQ+VWRN+  VGCA+VKC N    + CNY PPGN IGQ PY
Sbjct: 126 GHYTQVVWRNSVRVGCARVKCNNGGYVVSCNYDPPGNVIGQSPY 168

[Close]

BLAST of Lsi03G006220 vs. Swiss-Prot
Match: PR1A_SOLLC (Pathogenesis-related protein 1A1 OS=Solanum lycopersicum PE=2 SV=1)

HSP 1 Score: 154.5 bits (389), Expect = 1.0e-36
Identity = 71/134 (52.99%), Postives = 90/134 (67.16%), Query Frame = 1


Query: 27  AKSYPKNYIAAHNTIRVEVGVEPLHWNATLAAYAQNYANTKIATGQMEHSRGPYGENLAE 86
           A++  +N++ AHN  R  VGV P+ W+  LAAYAQNYAN +     M HS GPYGENLA 
Sbjct: 21  AQTPRENFLNAHNAARRRVGVGPMTWDDGLAAYAQNYANQRADDCGMIHSDGPYGENLAA 80

Query: 87  GYEVMTAETAVSLWADEKKHYDYNSNMCVNDSSHCLHYTQLVWRNTKSVGCAQVKCQNNW 146
            +  + A  AV +W DEK+ YDYNSN C      C HYTQ+VWR +  +GCA+V+C + W
Sbjct: 81  AFPQLNAAGAVKMWDDEKQWYDYNSNTCA-PGKVCGHYTQVVWRKSVRLGCARVRCNSGW 140

Query: 147 VFLICNYYPPGNYI 161
           VF+ CNY PPGNYI
Sbjct: 141 VFITCNYDPPGNYI 153

[Close]

BLAST of Lsi03G006220 vs. Swiss-Prot
Match: PRB1_ARATH (Pathogenesis-related protein 1 OS=Arabidopsis thaliana GN=PRB1 PE=2 SV=1)

HSP 1 Score: 152.5 bits (384), Expect = 4.0e-36
Identity = 71/158 (44.94%), Postives = 103/158 (65.19%), Query Frame = 1


Query: 8   SILFLIGLTLTLASIAPILAKSYPKNYIAAHNTIRVEVGVEPLHWNATLAAYAQNYANTK 67
           S + +I   L  A + P+ A+   ++Y+ AHN  R ++GV P+ W+  LAAYA+NYAN  
Sbjct: 7   SRILIILAALVGALVVPLKAQDSQQDYVNAHNQARSQIGVGPMQWDEGLAAYARNYANQL 66

Query: 68  IATGQMEHSRGPYGENLAEGYEVMTAETAVSLWADEKKHYDYNSNMCVNDSSHCLHYTQL 127
               ++ HSRGPYGENLA+    ++   AV+LW +EK +Y+Y++N C   +  C HYTQ+
Sbjct: 67  KGDCRLVHSRGPYGENLAKSGGDLSGVAAVNLWVNEKANYNYDTNTC---NGVCGHYTQV 126

Query: 128 VWRNTKSVGCAQVKCQNNWVFLICNYYPPGNYIGQRPY 166
           VWRN+  +GCA+V+C N    + CNY PPGNY  Q+PY
Sbjct: 127 VWRNSVRLGCAKVRCNNGGTIISCNYDPPGNYANQKPY 161

[Close]

BLAST of Lsi03G006220 vs. TrEMBL
Match: A0A0A0K7N5_CUCSA (Uncharacterized protein OS=Cucumis sativus GN=Csa_7G070232 PE=3 SV=1)

HSP 1 Score: 286.2 bits (731), Expect = 2.6e-74
Identity = 141/171 (82.46%), Postives = 146/171 (85.38%), Query Frame = 1


Query: 1   MTFANILSILFLIGLTLTL------ASIAPILAKSYPKNYIAAHNTIRVEVGVEPLHWNA 60
           M FANILS L L+GLTLTL      AS AP LAKS PKNYI AHN +R  VGVEPLHWN+
Sbjct: 1   MGFANILSTLCLLGLTLTLTLTLTLASTAPSLAKSSPKNYIDAHNAVRAAVGVEPLHWNS 60

Query: 61  TLAAYAQNYANTKIATGQMEHSRGPYGENLAEGYEVMTAETAVSLWADEKKHYDYNSNMC 120
           TLA YAQNYANTKIAT QMEHS GPYGENLAEG EVMTAETAVSLWADEKKHYDYNSN C
Sbjct: 61  TLADYAQNYANTKIATCQMEHSGGPYGENLAEGNEVMTAETAVSLWADEKKHYDYNSNTC 120

Query: 121 VNDSSHCLHYTQLVWRNTKSVGCAQVKCQNNWVFLICNYYPPGNYIGQRPY 166
            ND S+CLHYTQLVW NTKSVGCAQVKCQNNWVFLIC+YYPPGNY GQRPY
Sbjct: 121 SNDPSNCLHYTQLVWSNTKSVGCAQVKCQNNWVFLICSYYPPGNYNGQRPY 171

[Close]

BLAST of Lsi03G006220 vs. TrEMBL
Match: A0A0A0K4C1_CUCSA (Uncharacterized protein OS=Cucumis sativus GN=Csa_7G070225 PE=3 SV=1)

HSP 1 Score: 198.4 bits (503), Expect = 7.0e-48
Identity = 94/160 (58.75%), Postives = 117/160 (73.12%), Query Frame = 1


Query: 6   ILSILFLIGLTLTLASIAPILAKSYPKNYIAAHNTIRVEVGVEPLHWNATLAAYAQNYAN 65
           +L    ++GL   LASI   LA++  ++++ AHN  R +VGV P+ WN TLAAYAQ YAN
Sbjct: 7   LLISFMMMGLITQLASIT--LAQNSHQDFVNAHNAARAKVGVGPVSWNYTLAAYAQTYAN 66

Query: 66  TKIATGQMEHSRGPYGENLAEGYEVMTAETAVSLWADEKKHYDYNSNMCVNDSSHCLHYT 125
            KI T +M+HS GPYGENLAEGY  MTA  AV+ W  EKK+YD++SN C+ D   C HYT
Sbjct: 67  KKIGTCEMQHSYGPYGENLAEGYGEMTAVEAVNFWVSEKKYYDHHSNRCIGD--ECRHYT 126

Query: 126 QLVWRNTKSVGCAQVKCQNNWVFLICNYYPPGNYIGQRPY 166
           Q+VWR TK VGCA+VKC NNW+F+ICNY PP NY+GQ PY
Sbjct: 127 QVVWRGTKHVGCARVKCHNNWIFVICNYDPPDNYVGQFPY 162

[Close]

BLAST of Lsi03G006220 vs. TrEMBL
Match: A0A0A0K2C5_CUCSA (Uncharacterized protein OS=Cucumis sativus GN=Csa_7G070230 PE=4 SV=1)

HSP 1 Score: 193.7 bits (491), Expect = 1.7e-46
Identity = 87/154 (56.49%), Postives = 112/154 (72.73%), Query Frame = 1


Query: 12  LIGLTLTLASIAPILAKSYPKNYIAAHNTIRVEVGVEPLHWNATLAAYAQNYANTKIATG 71
           ++GL L LA I+P LA S P++++ AHN IR + GV P+ WN T+A+YA+ YA TK AT 
Sbjct: 13  VVGLFLILAPISPTLANSTPQDFVDAHNAIRAKYGVGPVTWNKTIASYAEKYAKTKTATC 72

Query: 72  QMEHSRGPYGENLAEGYEVMTAETAVSLWADEKKHYDYNSNMCVNDSSHCLHYTQLVWRN 131
           +MEHS GPYGENLAE +E  TAE  V+ WA E K YD+ SN CV +   C H+ Q+VW++
Sbjct: 73  EMEHSMGPYGENLAEAFEKTTAELTVNYWASEDKFYDHKSNKCVEE--ECGHFLQIVWKD 132

Query: 132 TKSVGCAQVKCQNNWVFLICNYYPPGNYIGQRPY 166
           T S+GCA+VKC NN++F ICNYYPPG Y  Q PY
Sbjct: 133 TTSIGCAEVKCNNNYIFTICNYYPPGGYPDQLPY 164

[Close]

BLAST of Lsi03G006220 vs. TrEMBL
Match: A0A0A0K6A0_CUCSA (Uncharacterized protein OS=Cucumis sativus GN=Csa_7G070235 PE=3 SV=1)

HSP 1 Score: 191.0 bits (484), Expect = 1.1e-45
Identity = 88/162 (54.32%), Postives = 117/162 (72.22%), Query Frame = 1


Query: 4   ANILSILFLIGLTLTLASIAPILAKSYPKNYIAAHNTIRVEVGVEPLHWNATLAAYAQNY 63
           + I  + FL+  T++ A       ++ P++++  HN IR  VGV P+ W+ TLAAYAQ+Y
Sbjct: 9   STIFLVSFLLATTISNA-------QNSPQDFVDTHNDIRAAVGVGPVSWDDTLAAYAQSY 68

Query: 64  ANTKIATGQMEHSRGPYGENLAEGYEVMTAETAVSLWADEKKHYDYNSNMCVNDSSHCLH 123
           A++K+ T +MEHS GPYGENLAEGY+ MT   AV  WA EKK Y+++ N CV D   C H
Sbjct: 69  ADSKMDTCEMEHSNGPYGENLAEGYDEMTGVEAVRFWATEKKFYNHHLNRCVGDE--CGH 128

Query: 124 YTQLVWRNTKSVGCAQVKCQNNWVFLICNYYPPGNYIGQRPY 166
           YTQ+VWR+T ++GC +VKC+NNWVF+ICNY PPGNYIGQ PY
Sbjct: 129 YTQIVWRHTTNIGCGRVKCENNWVFVICNYNPPGNYIGQHPY 161

[Close]

BLAST of Lsi03G006220 vs. TrEMBL
Match: A0A0L9TL64_PHAAN (Uncharacterized protein OS=Phaseolus angularis GN=LR48_Vigan01g073200 PE=3 SV=1)

HSP 1 Score: 189.9 bits (481), Expect = 2.5e-45
Identity = 82/139 (58.99%), Postives = 100/139 (71.94%), Query Frame = 1


Query: 27  AKSYPKNYIAAHNTIRVEVGVEPLHWNATLAAYAQNYANTKIATGQMEHSRGPYGENLAE 86
           A++ PK+Y+  HN  R EVGV+PL WN TL AY Q Y N+K  T + EHS GPYGENLA+
Sbjct: 7   AQNSPKDYLDEHNKARKEVGVKPLKWNETLVAYGQKYVNSKKKTCEFEHSMGPYGENLAQ 66

Query: 87  GYEVMTAETAVSLWADEKKHYDYNSNMCVNDSSHCLHYTQLVWRNTKSVGCAQVKCQNNW 146
           GY   +   +V +W  EK +YD  +N CV D   CLHYTQ+VW  T+SVGCA+ KC NNW
Sbjct: 67  GYGEFSGVDSVKMWVGEKPNYDEKNNKCVKD--ECLHYTQVVWNTTESVGCARTKCDNNW 126

Query: 147 VFLICNYYPPGNYIGQRPY 166
           +F+ICNYYPPGNYIG RPY
Sbjct: 127 MFIICNYYPPGNYIGSRPY 143

[Close]

BLAST of Lsi03G006220 vs. TAIR10
Match: AT4G33720.1 (AT4G33720.1 CAP (Cysteine-rich secretory proteins, Antigen 5, and Pathogenesis-related 1 protein) superfamily protein)

HSP 1 Score: 158.7 bits (400), Expect = 3.1e-39
Identity = 78/165 (47.27%), Postives = 104/165 (63.03%), Query Frame = 1


Query: 1   MTFANILSILFLIGLTLTLASIAPILAKSYPKNYIAAHNTIRVEVGVEPLHWNATLAAYA 60
           M   N    LFL  +T  L  I  + A+  P++++A HN  R EVGV PL W+  +AAYA
Sbjct: 1   MKIFNSSQNLFL-AITFFLVLIVHLKAQDSPQDFLAVHNRARAEVGVGPLRWDEKVAAYA 60

Query: 61  QNYANTKIATGQMEHSRGPYGENLAEGYEVMTAETAVSLWADEKKHYDYNSNMCVNDSSH 120
           +NYAN +     M+HS G YGEN+A     MT   AV +W DE+  YDY+SN C  D   
Sbjct: 61  RNYANQRKGDCAMKHSSGSYGENIAWSSGSMTGVAAVDMWVDEQFDYDYDSNTCAWD-KQ 120

Query: 121 CLHYTQLVWRNTKSVGCAQVKCQNNWVFLICNYYPPGNYIGQRPY 166
           C HYTQ+VWRN++ +GCA+V+C N   F+ CNY PPGN++G+ PY
Sbjct: 121 CGHYTQVVWRNSERLGCAKVRCNNGQTFITCNYDPPGNWVGEWPY 163

[Close]

BLAST of Lsi03G006220 vs. TAIR10
Match: AT3G19690.1 (AT3G19690.1 CAP (Cysteine-rich secretory proteins, Antigen 5, and Pathogenesis-related 1 protein) superfamily protein)

HSP 1 Score: 158.3 bits (399), Expect = 4.1e-39
Identity = 74/159 (46.54%), Postives = 107/159 (67.30%), Query Frame = 1


Query: 8   SILFLIGLTLTLASIAPILAKSYPKNYIAAHNTIRVEVGVEPLHWNATLAAYAQNYANTK 67
           +ILFL+ + L   S    LA+   + ++ AHN  R EVG++PL W+  +AAYA +YAN +
Sbjct: 7   NILFLLAIALFYGS----LAEDLQQQFLEAHNEARNEVGLDPLVWDDEVAAYAASYANQR 66

Query: 68  IATGQMEHSRGPYGENLAEGYEVMTAETAVSLWADEKKHYDYNSNMCVN-DSSHCLHYTQ 127
           I    + HS GP+GEN+A     M+AE A  +W +EK++YDY+SN C + +   CLHYTQ
Sbjct: 67  INDCALVHSNGPFGENIAMSSGEMSAEDAAEMWINEKQYYDYDSNTCNDPNGGTCLHYTQ 126

Query: 128 LVWRNTKSVGCAQVKCQNNWVFLICNYYPPGNYIGQRPY 166
           +VW+NT  +GCA+V C +   F+ CNY PPGNYIG++P+
Sbjct: 127 VVWKNTVRLGCAKVVCNSGGTFITCNYDPPGNYIGEKPF 161

[Close]

BLAST of Lsi03G006220 vs. TAIR10
Match: AT2G19990.1 (AT2G19990.1 pathogenesis-related protein-1-like)

HSP 1 Score: 154.8 bits (390), Expect = 4.5e-38
Identity = 69/136 (50.74%), Postives = 94/136 (69.12%), Query Frame = 1


Query: 31  PKNYIAAHNTIRVEVGVEPLHWNATLAAYAQNYANTKIATGQMEHSRGPYGENLAEGYEV 90
           P+  +  HN  R  VGV P+ WN TLA YAQ+YA+ +     M+HS GP+GENLA G+  
Sbjct: 42  PQETLVVHNKARAMVGVGPMVWNETLATYAQSYAHERARDCAMKHSLGPFGENLAAGWGT 101

Query: 91  MTAETAVSLWADEKKHYDYNSNMCVNDSSHCLHYTQLVWRNTKSVGCAQVKCQNN-WVFL 150
           M+   A   W  EK++YDY+SN C  D   C HYTQ+VWR++  +GCA V+C+N+ ++++
Sbjct: 102 MSGPVATEYWMTEKENYDYDSNTCGGDGV-CGHYTQIVWRDSVRLGCASVRCKNDEYIWV 161

Query: 151 ICNYYPPGNYIGQRPY 166
           IC+Y PPGNYIGQRPY
Sbjct: 162 ICSYDPPGNYIGQRPY 176

[Close]

BLAST of Lsi03G006220 vs. TAIR10
Match: AT1G50060.1 (AT1G50060.1 CAP (Cysteine-rich secretory proteins, Antigen 5, and Pathogenesis-related 1 protein) superfamily protein)

HSP 1 Score: 152.9 bits (385), Expect = 1.7e-37
Identity = 74/162 (45.68%), Postives = 105/162 (64.81%), Query Frame = 1


Query: 5   NILSILFLIGLTLTLASIAPILAKSYPKNYIAAHNTIRVEVGVEPLHWNATLAAYAQNYA 64
           N     FL+ + ++   +A   A++ P++Y+ +HNT R +VGV  + W+ TLAAYA NY+
Sbjct: 2   NTFKTPFLVIVAISFLVVATN-AQNTPQDYLNSHNTARAQVGVPNVVWDTTLAAYALNYS 61

Query: 65  NTKIATGQMEHSRGPYGENLAEGYEV-MTAETAVSLWADEKKHYDYNSNMCVNDSSHCLH 124
           N + A   + HS GPYGENLA+G     +A +AV LW DEK +Y Y  N C      CLH
Sbjct: 62  NFRKADCNLVHSNGPYGENLAKGSSSSFSAISAVKLWVDEKPYYSYAYNNCTG-GKQCLH 121

Query: 125 YTQLVWRNTKSVGCAQVKCQNNWVFLICNYYPPGNYIGQRPY 166
           YTQ+VWR++  +GCA+V+C N W F+ CNY  PGN++G+ PY
Sbjct: 122 YTQVVWRDSVKIGCARVQCTNTWWFVSCNYNSPGNWVGEYPY 161

[Close]

BLAST of Lsi03G006220 vs. TAIR10
Match: AT2G14580.1 (AT2G14580.1 basic pathogenesis-related protein 1)

HSP 1 Score: 152.5 bits (384), Expect = 2.2e-37
Identity = 71/158 (44.94%), Postives = 103/158 (65.19%), Query Frame = 1


Query: 8   SILFLIGLTLTLASIAPILAKSYPKNYIAAHNTIRVEVGVEPLHWNATLAAYAQNYANTK 67
           S + +I   L  A + P+ A+   ++Y+ AHN  R ++GV P+ W+  LAAYA+NYAN  
Sbjct: 7   SRILIILAALVGALVVPLKAQDSQQDYVNAHNQARSQIGVGPMQWDEGLAAYARNYANQL 66

Query: 68  IATGQMEHSRGPYGENLAEGYEVMTAETAVSLWADEKKHYDYNSNMCVNDSSHCLHYTQL 127
               ++ HSRGPYGENLA+    ++   AV+LW +EK +Y+Y++N C   +  C HYTQ+
Sbjct: 67  KGDCRLVHSRGPYGENLAKSGGDLSGVAAVNLWVNEKANYNYDTNTC---NGVCGHYTQV 126

Query: 128 VWRNTKSVGCAQVKCQNNWVFLICNYYPPGNYIGQRPY 166
           VWRN+  +GCA+V+C N    + CNY PPGNY  Q+PY
Sbjct: 127 VWRNSVRLGCAKVRCNNGGTIISCNYDPPGNYANQKPY 161

[Close]

BLAST of Lsi03G006220 vs. NCBI nr
Match: gi|659110139|ref|XP_008455069.1| (PREDICTED: basic form of pathogenesis-related protein 1-like [Cucumis melo])

HSP 1 Score: 304.7 bits (779), Expect = 1.0e-79
Identity = 143/165 (86.67%), Postives = 151/165 (91.52%), Query Frame = 1


Query: 1   MTFANILSILFLIGLTLTLASIAPILAKSYPKNYIAAHNTIRVEVGVEPLHWNATLAAYA 60
           M FANILS   L+GLTLTLAS APILAKSYPKNY+ AHN IR EVGV+PLHWN+TLAAYA
Sbjct: 1   MGFANILSTFCLLGLTLTLASTAPILAKSYPKNYVDAHNAIRAEVGVDPLHWNSTLAAYA 60

Query: 61  QNYANTKIATGQMEHSRGPYGENLAEGYEVMTAETAVSLWADEKKHYDYNSNMCVNDSSH 120
           QNYANTKIAT QMEHS GPYGENLAEGYE MTAE AVSLWADEKKHYDYNSN C ND+S+
Sbjct: 61  QNYANTKIATCQMEHSGGPYGENLAEGYEEMTAEMAVSLWADEKKHYDYNSNTCTNDASN 120

Query: 121 CLHYTQLVWRNTKSVGCAQVKCQNNWVFLICNYYPPGNYIGQRPY 166
           CLHYTQLVWRNTKSVGCA+VKCQNNWVFLIC+YYPPGNYIGQRPY
Sbjct: 121 CLHYTQLVWRNTKSVGCAEVKCQNNWVFLICSYYPPGNYIGQRPY 165

[Close]

BLAST of Lsi03G006220 vs. NCBI nr
Match: gi|778724756|ref|XP_004137080.2| (PREDICTED: pathogenesis-related protein 1A-like [Cucumis sativus])

HSP 1 Score: 286.2 bits (731), Expect = 3.7e-74
Identity = 141/171 (82.46%), Postives = 146/171 (85.38%), Query Frame = 1


Query: 1   MTFANILSILFLIGLTLTL------ASIAPILAKSYPKNYIAAHNTIRVEVGVEPLHWNA 60
           M FANILS L L+GLTLTL      AS AP LAKS PKNYI AHN +R  VGVEPLHWN+
Sbjct: 1   MGFANILSTLCLLGLTLTLTLTLTLASTAPSLAKSSPKNYIDAHNAVRAAVGVEPLHWNS 60

Query: 61  TLAAYAQNYANTKIATGQMEHSRGPYGENLAEGYEVMTAETAVSLWADEKKHYDYNSNMC 120
           TLA YAQNYANTKIAT QMEHS GPYGENLAEG EVMTAETAVSLWADEKKHYDYNSN C
Sbjct: 61  TLADYAQNYANTKIATCQMEHSGGPYGENLAEGNEVMTAETAVSLWADEKKHYDYNSNTC 120

Query: 121 VNDSSHCLHYTQLVWRNTKSVGCAQVKCQNNWVFLICNYYPPGNYIGQRPY 166
            ND S+CLHYTQLVW NTKSVGCAQVKCQNNWVFLIC+YYPPGNY GQRPY
Sbjct: 121 SNDPSNCLHYTQLVWSNTKSVGCAQVKCQNNWVFLICSYYPPGNYNGQRPY 171

[Close]

BLAST of Lsi03G006220 vs. NCBI nr
Match: gi|659110143|ref|XP_008455071.1| (PREDICTED: basic form of pathogenesis-related protein 1-like [Cucumis melo])

HSP 1 Score: 200.7 bits (509), Expect = 2.0e-48
Identity = 96/165 (58.18%), Postives = 120/165 (72.73%), Query Frame = 1


Query: 1   MTFANILSILFLIGLTLTLASIAPILAKSYPKNYIAAHNTIRVEVGVEPLHWNATLAAYA 60
           M    +L I F++   +TLA I   LA++  ++++ AHN  R +VGV P+ WN TLAAYA
Sbjct: 1   MPLPKLLLISFMMMGLITLAPIT--LAQNSHQDFVNAHNNARAKVGVGPVSWNYTLAAYA 60

Query: 61  QNYANTKIATGQMEHSRGPYGENLAEGYEVMTAETAVSLWADEKKHYDYNSNMCVNDSSH 120
           Q YAN KI T +M+HS GPYGENLAEGY  MTA  AV+ W  EKK+YD++SN C+ D   
Sbjct: 61  QTYANKKIGTCEMQHSYGPYGENLAEGYGEMTAVEAVNFWVSEKKYYDHHSNRCIGD--E 120

Query: 121 CLHYTQLVWRNTKSVGCAQVKCQNNWVFLICNYYPPGNYIGQRPY 166
           C HYTQ+VWR TK VGCA+VKC NNW+F+ICNY PPGNY+GQ PY
Sbjct: 121 CRHYTQVVWRGTKHVGCARVKCHNNWIFVICNYDPPGNYVGQFPY 161

[Close]

BLAST of Lsi03G006220 vs. NCBI nr
Match: gi|449438289|ref|XP_004136921.1| (PREDICTED: basic form of pathogenesis-related protein 1-like [Cucumis sativus])

HSP 1 Score: 198.4 bits (503), Expect = 1.0e-47
Identity = 94/160 (58.75%), Postives = 117/160 (73.12%), Query Frame = 1


Query: 6   ILSILFLIGLTLTLASIAPILAKSYPKNYIAAHNTIRVEVGVEPLHWNATLAAYAQNYAN 65
           +L    ++GL   LASI   LA++  ++++ AHN  R +VGV P+ WN TLAAYAQ YAN
Sbjct: 7   LLISFMMMGLITQLASIT--LAQNSHQDFVNAHNAARAKVGVGPVSWNYTLAAYAQTYAN 66

Query: 66  TKIATGQMEHSRGPYGENLAEGYEVMTAETAVSLWADEKKHYDYNSNMCVNDSSHCLHYT 125
            KI T +M+HS GPYGENLAEGY  MTA  AV+ W  EKK+YD++SN C+ D   C HYT
Sbjct: 67  KKIGTCEMQHSYGPYGENLAEGYGEMTAVEAVNFWVSEKKYYDHHSNRCIGD--ECRHYT 126

Query: 126 QLVWRNTKSVGCAQVKCQNNWVFLICNYYPPGNYIGQRPY 166
           Q+VWR TK VGCA+VKC NNW+F+ICNY PP NY+GQ PY
Sbjct: 127 QVVWRGTKHVGCARVKCHNNWIFVICNYDPPDNYVGQFPY 162

[Close]

BLAST of Lsi03G006220 vs. NCBI nr
Match: gi|659110141|ref|XP_008455070.1| (PREDICTED: basic form of pathogenesis-related protein 1-like [Cucumis melo])

HSP 1 Score: 197.2 bits (500), Expect = 2.2e-47
Identity = 89/159 (55.97%), Postives = 117/159 (73.58%), Query Frame = 1


Query: 7   LSILFLIGLTLTLASIAPILAKSYPKNYIAAHNTIRVEVGVEPLHWNATLAAYAQNYANT 66
           +S   ++GL+L LA I+P +AKS P+ ++ AHN IR + GV P+ WN TLA+YA+NYA T
Sbjct: 8   VSAFCVVGLSLILAPISPTIAKSSPQEFVDAHNAIRAKYGVGPVTWNKTLASYAENYAKT 67

Query: 67  KIATGQMEHSRGPYGENLAEGYEVMTAETAVSLWADEKKHYDYNSNMCVNDSSHCLHYTQ 126
           ++AT +MEHS GPYGENLAE +E  TAE  V+ WA E K YD+ SN CV +   C H+ Q
Sbjct: 68  RVATCEMEHSMGPYGENLAEAFEKTTAELTVNYWATEDKFYDHKSNKCVEE--ECGHFLQ 127

Query: 127 LVWRNTKSVGCAQVKCQNNWVFLICNYYPPGNYIGQRPY 166
           +VW++T S+GCA+VKC NN++F ICNYYPPG Y  Q PY
Sbjct: 128 VVWKDTTSIGCAEVKCNNNYIFTICNYYPPGGYPDQLPY 164

The following BLAST results are available for this feature:

BLAST of Lsi03G006220 vs. Swiss-Prot


Match Name	E-value	Identity	Description
PRB1_TOBAC	1.4e-41	54.68	Basic form of pathogenesis-related protein 1 OS=Nicotiana tabacum PE=3 SV=1	[more]
PR1A_TOBAC	4.2e-38	49.39	Pathogenesis-related protein 1A OS=Nicotiana tabacum PE=1 SV=1	[more]
PR1B_TOBAC	6.1e-37	48.78	Pathogenesis-related protein 1B OS=Nicotiana tabacum PE=2 SV=1	[more]
PR1A_SOLLC	1.0e-36	52.99	Pathogenesis-related protein 1A1 OS=Solanum lycopersicum PE=2 SV=1	[more]
PRB1_ARATH	4.0e-36	44.94	Pathogenesis-related protein 1 OS=Arabidopsis thaliana GN=PRB1 PE=2 SV=1	[more]

BLAST of Lsi03G006220 vs. TrEMBL


Match Name	E-value	Identity	Description
A0A0A0K7N5_CUCSA	2.6e-74	82.46	Uncharacterized protein OS=Cucumis sativus GN=Csa_7G070232 PE=3 SV=1	[more]
A0A0A0K4C1_CUCSA	7.0e-48	58.75	Uncharacterized protein OS=Cucumis sativus GN=Csa_7G070225 PE=3 SV=1	[more]
A0A0A0K2C5_CUCSA	1.7e-46	56.49	Uncharacterized protein OS=Cucumis sativus GN=Csa_7G070230 PE=4 SV=1	[more]
A0A0A0K6A0_CUCSA	1.1e-45	54.32	Uncharacterized protein OS=Cucumis sativus GN=Csa_7G070235 PE=3 SV=1	[more]
A0A0L9TL64_PHAAN	2.5e-45	58.99	Uncharacterized protein OS=Phaseolus angularis GN=LR48_Vigan01g073200 PE=3 SV=1	[more]

BLAST of Lsi03G006220 vs. TAIR10


Match Name	E-value	Identity	Description
AT4G33720.1	3.1e-39	47.27	CAP (Cysteine-rich secretory proteins, Antigen 5, and Pathogenesis-r...	[more]
AT3G19690.1	4.1e-39	46.54	CAP (Cysteine-rich secretory proteins, Antigen 5, and Pathogenesis-r...	[more]
AT2G19990.1	4.5e-38	50.74	pathogenesis-related protein-1-like	[more]
AT1G50060.1	1.7e-37	45.68	CAP (Cysteine-rich secretory proteins, Antigen 5, and Pathogenesis-r...	[more]
AT2G14580.1	2.2e-37	44.94	basic pathogenesis-related protein 1	[more]

BLAST of Lsi03G006220 vs. NCBI nr


Match Name	E-value	Identity	Description
gi\|659110139\|ref\|XP_008455069.1\|	1.0e-79	86.67	PREDICTED: basic form of pathogenesis-related protein 1-like [Cucumis melo]	[more]
gi\|778724756\|ref\|XP_004137080.2\|	3.7e-74	82.46	PREDICTED: pathogenesis-related protein 1A-like [Cucumis sativus]	[more]
gi\|659110143\|ref\|XP_008455071.1\|	2.0e-48	58.18	PREDICTED: basic form of pathogenesis-related protein 1-like [Cucumis melo]	[more]
gi\|449438289\|ref\|XP_004136921.1\|	1.0e-47	58.75	PREDICTED: basic form of pathogenesis-related protein 1-like [Cucumis sativus]	[more]
gi\|659110141\|ref\|XP_008455070.1\|	2.2e-47	55.97	PREDICTED: basic form of pathogenesis-related protein 1-like [Cucumis melo]	[more]

Annotated Terms

The following terms have been associated with this gene:

Vocabulary: **Cellular Component**
Term	Definition
GO:0005576	extracellular region

Vocabulary: **INTERPRO**
Term	Definition
IPR018244	Allrgn_V5/Tpx1_CS
IPR014044	CAP_domain
IPR001283	Allrgn_V5/Tpx1

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0008150	biological_process
cellular_component	GO:0005576	extracellular region
cellular_component	GO:0005575	cellular_component
molecular_function	GO:0003674	molecular_function

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Lsi03G006220.1	Lsi03G006220.1	mRNA

InterPro

Analysis Name: InterPro Annotations of Lagenaria siceraria
Date Performed: 2017-09-18


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR001283	Cysteine-rich secretory protein, allergen V5/Tpx-1-related	PRINTS	PR00837	V5TPXLIKE	coord: 50..68 score: 1.2E-18coord: 96..109 score: 1.2E-18coord: 121..137 score: 1.2E-18coord: 148..161 score: 1.2
IPR001283	Cysteine-rich secretory protein, allergen V5/Tpx-1-related	PANTHER	PTHR10334	CYSTEINE-RICH SECRETORY PROTEIN-RELATED	coord: 1..165 score: 1.5
IPR014044	CAP domain	GENE3D	G3DSA:3.40.33.10		coord: 28..165 score: 1.4
IPR014044	CAP domain	PFAM	PF00188	CAP	coord: 36..153 score: 2.8
IPR014044	CAP domain	SMART	SM00198	SCP_3	coord: 29..161 score: 9.8
IPR014044	CAP domain	unknown	SSF55797	PR-1-like	coord: 9..165 score: 1.96
IPR018244	Allergen V5/Tpx-1-related, conserved site	PROSITE	PS01010	CRISP_2	coord: 148..159 scor
None	No IPR available	PANTHER	PTHR10334:SF223	F2J10.6 PROTEIN-RELATED	coord: 1..165 score: 1.5

Synteny

The following gene(s) are orthologous to this gene:


Gene	Orthologue	Organism	Block
Lsi03G006220	CSPI07G06600	Wild cucumber (PI 183967)	cpilsiB536
Lsi03G006220	Cucsa.218900	Cucumber (Gy14) v1	cgylsiB367
Lsi03G006220	CmoCh11G014310	Cucurbita moschata (Rifu)	cmolsiB103
Lsi03G006220	CmoCh19G005320	Cucurbita moschata (Rifu)	cmolsiB468
Lsi03G006220	Cla001629	Watermelon (97103) v1	lsiwmB268
Lsi03G006220	ClCG02G007260	Watermelon (Charleston Gray)	lsiwcgB233

The following gene(s) are paralogous to this gene:

None

The following block(s) are covering this gene:


Gene	Organism	Block
Lsi03G006220	Cucurbita maxima (Rimu)	cmalsiB116
Lsi03G006220	Cucurbita maxima (Rimu)	cmalsiB484
Lsi03G006220	Cucurbita maxima (Rimu)	cmalsiB662
Lsi03G006220	Cucurbita moschata (Rifu)	cmolsiB650
Lsi03G006220	Cucurbita pepo (Zucchini)	cpelsiB208
Lsi03G006220	Cucurbita pepo (Zucchini)	cpelsiB543
Lsi03G006220	Cucumber (Chinese Long) v2	culsiB522
Lsi03G006220	Melon (DHL92) v3.5.1	lsimeB247
Lsi03G006220	Melon (DHL92) v3.5.1	lsimeB268
Lsi03G006220	Watermelon (Charleston Gray)	lsiwcgB223
Lsi03G006220	Watermelon (97103) v1	lsiwmB245
Lsi03G006220	Melon (DHL92) v3.6.1	lsimedB260
Lsi03G006220	Melon (DHL92) v3.6.1	lsimedB281
Lsi03G006220	Cucumber (Chinese Long) v3	cuclsiB555
Lsi03G006220	Watermelon (97103) v2	lsiwmbB226
Lsi03G006220	Wax gourd	lsiwgoB307
Lsi03G006220	Wax gourd	lsiwgoB320
Lsi03G006220	Bottle gourd (USVL1VR-Ls)	lsilsiB126