Lsi03G004910 (gene) Bottle gourd (USVL1VR-Ls) | Cucurbit Genomics Database (CuGenDB)

Overview


Name	Lsi03G004910
Type	gene
Organism	Lagenaria siceraria (Bottle gourd (USVL1VR-Ls))
Description	LAG1 longevity assurance like 2
Location	chr03 : 5605910 .. 5610826 (-)

Genome Viewer

Gene Expression

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: five_prime_UTRCDS

Hold the cursor over a type above to highlight its positions in the sequence below.

AAAAAAAAAAAATGAAAAAAGAAAAAAAAAAAAACACAACGAGATCTCAACCTCCACTTCCTGCTTATTCTCTTCCTTCCATTCCTTCTTTCCTCTTCTCAAATTTCCCAGCCTTTTCACTCTCACTTCTCCAATCACCTCTCTGCAAATTCTAGGGCAAAACTTAGATCTCTTGCGACGAAGAAGCTTTTTCTACGCTTACCTCTATTGATTTCGCTTCTCTCATTCTAGGTTCTTTCACCAAATTTTCCTTTTTCTTTTCTTCCCCTTCGTTTTTCATGCTTCTCCTTTAAATTTTACCTTCAATGGACTCGATATGGAGCCACAATAGCCCCCCAGCCGCGTTACATTTCCTTATCGCCCTCCACTACGCATTCGCCTTCTTCGCCGCTAGGTTCTTACTCGACAGATTCATTTTTCGTGTGAGTTTTCTTAATTTTTCTTTTACTCTTTCTCTACTGCTATTTTGTTTCTCCTTTTCCTTTTGTTTTATTGATATGGATTCCGATCATTATGTAGCAATTTATTTGTATATAGGGTTGGATGAATTGTTTTGTGCGATGGTGGAAATGTGAAGAATTTAAGACGAGATGGAATTTTTTTTAAAACGTCTCTGATTGTGCTACTTGCTTCTAAGTGATACGTTAAGAACGTTGTACTCGATTTTTCTTCTTTCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTATTTTTTTTTATCTAAGAAGAAGATGAGAAGGGCTTGGTTATGTTATCCTCGGTGAATTTCAAATGCTAGTTCTCTACTGAACTTAATCGGTGGTGTGTTCGACATTTACTTAGTTTGGTTGTCTTATTATAGAGCATCTGGTTCCTTGGTGACTTGGCCAGACGTGAAATACTTGGTTGTAAATAATATGTATTAGGACTTGTTGCCTCCTTAGCTTCAATGAAGATGTCTCAGAAGTTTAGTTGGTCATCATTGATGAAATTTTAGAAATTTTACCTTTGTCCTGCTAGCCAAATTTCAGACGAAATTCAATTCCAACAGCACAAATTAATGCAATAGAAAAAGATTTTTGACTGGCTTTTATTTAGGGCAATGTAAGAACAGAAATCTAGTCATCAGAAAAGTAGCAAAAGGGAAACATTGAAGTGGTTAGACTTCAAGCCTCCCCCCACCTATGACACTTGTCCTCAAAAGATCATCCAAATCCTCCACAAAAAGTGGAGTTGAATAGAATTTCCTGTCATGCCACATCTTATCAGAAATACAATTTAGCTGAGCGCTGGTTGAAAAGGGAAACACCAGATAAATTATTCAAATCCTCGGTAGCCTCCTTGTAAAGATTACAACTATGATTAAAGAAAAATGATAGAGGGTCAAGCTCTAGGTAGTGTGAACAGTGATGATTCTCCCACCTTTTACATTATTCTCACTGCTCAACTCCTGGGAAAAGATGAATGCATTTGATGGGGAAGAGGTTAAACTAGGCTGACCAGTAAAGTTACCAAAATGCAGATCCCACTCTCTTGTCCCCAAGTTGATGATTGTGATGAGCCAATGACAGCATCTTGTCCTCGAGTGTTTCATCTTCTACTGAAAGAATACAAAGCCCACACTTTATTATCTCCAAATGGATGCACTCAAATGATGAGAAGATCAAATGGATTGTAACTGTAATAAAGCTAAAGAAGCATTCTAGTTTCCATTCAATTAGGGGGTGTTTGGGGAGGAATTGAGTGGGGTGGGGTGGGGTGGAGTGAAGTTATTAACTCCACTCCTTGTTTGGCAAGTTACTAACATCAATTCCACAATTTATTAACTCCTTGCACTATGGGCCCACGACTAAATAACTCTTTAGACTAAACAACTTCACTCCTAACCCCAAACGCCCTCTTAATATTTATATTTAGAAAAATTCTTATACATTTGGTTACCTTTTGAAATTCACATTGTTATTCCTATCTATAAAAATTCAGATTGTCATGTACTTGACATTTTTTCAGTTAAAAGTAGAAATGTGATTGGAAAAACTGTTGCACACCATAGAGCTTCTCTCCCGAGGTCTTTTCACAACGCATTTTGTAACTATAGGTTTTCCCTTTTTTTTTTTCTTTTAGTTGCTGCCAATCAGGGGAACCTATTTGTAATTCTCTGAACTTTGCAGTTGATTTCTTATCACCCTTTTTGTACTTGTTTCTTTAGCATTGTCAATAAGATTTCTCTGTTACTTATTACAAAAGGGGAAATAATGATGTTATTCTACATTTCTACTTGTTCTGTTTTCAGAGGTTGGCCATTTGGCTGTTGAGCAAAGGATCTGCCCCACTGAAACTTGACGAGGCTACCCAGTCAAAGGTTGTAAAATGTTCAGAATCAATGTGGAAACTAGCATACTATGGCACTGTTGAAATATGCATTCTAAAAATTGCATACAATGAACCATGGTTTAGAGATTCAAATCAGTACTTCAAAGGCTGGCCGAATCAGGAGTTGCAGTGAGTGGTTTACATGCTTTGATGTTAATTTAATTGAGGAACTTCATCTTTCGTCAACTAGCAGATCTAACTTGCTGTATGTATTGATATTATTGATAGAAATGCTTGAAGTGGGTTGGTGCCTCCTTCTATCTAGATTACTTCTCCTCAATATTTTTTGAAGACTTCTTTTCCTTCATTAGTGCATTTGGGCTCTTTGCTCCATTCTTCATCTTCCTTATTATGGCAACTCTAATACTAAGAAGTTGAAATTTTTACTTCTAACTTTAGTGCATTATTACTTTATTTTTTTTTTTTTTAAATTTATTTATAGAGAGGTGTATTATTACTGTATTATTACTTTTAAGGAATACATTCAAATGAGTTAAAGATTTTTAGTTGTATTTGAACTGATCCCTTTCACTTCTTTTTGCAGACTTCCTCTAAAACTGCTCTATATGTGCCAATGTGGGTTTTACTTATATAGCATCGCTGCGCTTCTTATATGGGAGACTCGGAGAAAGGATTTCTCCGTCATGATGTCTCATCATGTAATTACTGTCATCTTGATCGGATACTCATATATTACAAGGTATTCTTAAAGTTGTTTTCTACAAGTTCAAATATCTAGTAGTATTTACTAATAACTAGAAATATGTTGGTGGCCATTTCACAGATTGGGAAAATCATGCCAGAGTCTGTTTCCCATTTATGCTTTTATGGGCCTTGTTATATTGTTGCTGGGCTGCTGCTTCTTTTTCCTTTCTTTTTTTTTCTTGGGAGAAAGGTGGCCACCAGTCTTAAGATCTTGTAATGTTTGATATAGAATAGATCAAAGATTCAAAGCTGTGCTTGTTGATGGCTATTGCATTAGAACGAATGTTATTTTGTGGCCTTTTCTCTTCCTCTATCTCTCTCAAATTAATCTTCATATAACATGATACTAATCTGTTGTTTTGAGTATTCGTAGAAGCAATGTCTTTAATTTTCTGTGAAGTCAATGAAGTGTGTCTCTCACTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTATATATATATATATATATATATATATATATATATATATATATATTTGGCTCTAAATAGTACTTTATCTCTAACTTTTACTTTGACATTTATCATTCTAGTGCCTTTTCATGAAGTGATGGAAACCTTCTGAATTGAAGTTTATTTTATTAAAATCCTGCATTATTATGGCTGTCTTGCTCATCCTAATTATGCGTTGAGTTCATATACTGTTATTGAAAGATTGCAGTGTGTTTTTTGACAGTTTGCAGGATGACACTCTTGCCATGCATCATTAAATATAAAATAAGAGCATGCTATCAGAATAAGGAGATATTCAAGTTTAAATGTCTTCTATTTTTTAAAAAGATTGTTTGGATCTTATGAAATTTTTTCGTTTTTCTTTAAAAACTAAACAAAACTAAAGGGCTTTTCACATTTCCTCTCAAATTTTGATAAAAGCGTGTTTGTTCCTTTTTCTGATTTTGACTGTAGTCCTGATCTGATGGTAGTTAATAACCCATTAACAACGAGCATATAGATATCAACTATGATGGTGGTATTTATTATTATCTTTTTAAAACTCTACTTGGATTGAAAGTAATGCAAAGACTTGCTCATGACAAACAAAAGTTTTTAGATGTTTTTTTTGGGATAATGTACACACCTCTTATAGCGTCAATTTGGGGCTCCGTGAGTTCCTTTTTTTACCTTGATTATGTTGATGTCACTGTAACAATGTGTGAAAGTTATGCACATGTTTGCATAACTTTATATTTGTGTAATCAGGGGTCAAAATTTGTTTTAAAGCCTCTTCTGATTTTTTTTTTTGGTGGCTATATTTGGGTGTGTGTAGCCAAAAATTGCTTGACTATTCATTGTTTCTCAGCGTTTCTTATGATGGAAGCGTAGACTGATTATAATAATTTATTACATTTTAAATCAGAATTTTGTACTCAACTTGCAGATTTTTCCAGATTGGATCGGTTATTCTTGCGCTCCATGATGCAAGTGATGTCTTCATGGAAGCTGCCAAGGTTTTTAAATATTCTGAGAAAGAACTTGGAGCAAGTATTTTCTTTGGATTTTTTGCCATTTCATGGCTCATATTGCGGCTTATATTCTTCCCATTCTGGGTTATAAGAGCAACAAGGTTTGTGCTCCTAGTGTCTGATTTATCTATGAAGATTAGGCTCAATACTCTCTCTTTTTCCTATCTGATTTTTTGATTGATACTTCTCAGCTATGATCTCTGTGAGTACTTGAAGTTATCAGATGTGAATTCGAGATTAATATACTATGTTTTCAACACGATGTTGCTGATGTTGCTTGTGTTCCACATATACTGGTGGTTACTCATATGCTCTATGATTAGTAGACAGCTGAAAAACAGGGGAAAAGTTGGAGAAGATATAAGATCTGGTATGTAA

mRNA sequence

AAAAAAAAAAAATGAAAAAAGAAAAAAAAAAAAACACAACGAGATCTCAACCTCCACTTCCTGCTTATTCTCTTCCTTCCATTCCTTCTTTCCTCTTCTCAAATTTCCCAGCCTTTTCACTCTCACTTCTCCAATCACCTCTCTGCAAATTCTAGGGCAAAACTTAGATCTCTTGCGACGAAGAAGCTTTTTCTACGCTTACCTCTATTGATTTCGCTTCTCTCATTCTAGGTTCTTTCACCAAATTTTCCTTTTTCTTTTCTTCCCCTTCGTTTTTCATGCTTCTCCTTTAAATTTTACCTTCAATGGACTCGATATGGAGCCACAATAGCCCCCCAGCCGCGTTACATTTCCTTATCGCCCTCCACTACGCATTCGCCTTCTTCGCCGCTAGGTTCTTACTCGACAGATTCATTTTTCGTAGGTTGGCCATTTGGCTGTTGAGCAAAGGATCTGCCCCACTGAAACTTGACGAGGCTACCCAGTCAAAGGTTGTAAAATGTTCAGAATCAATGTGGAAACTAGCATACTATGGCACTGTTGAAATATGCATTCTAAAAATTGCATACAATGAACCATGGTTTAGAGATTCAAATCAGTACTTCAAAGGCTGGCCGAATCAGGAGTTGCAACTTCCTCTAAAACTGCTCTATATGTGCCAATGTGGGTTTTACTTATATAGCATCGCTGCGCTTCTTATATGGGAGACTCGGAGAAAGGATTTCTCCGTCATGATGTCTCATCATGTAATTACTGTCATCTTGATCGGATACTCATATATTACAAGATTTTTCCAGATTGGATCGGTTATTCTTGCGCTCCATGATGCAAGTGATGTCTTCATGGAAGCTGCCAAGGTTTTTAAATATTCTGAGAAAGAACTTGGAGCAAGTATTTTCTTTGGATTTTTTGCCATTTCATGGCTCATATTGCGGCTTATATTCTTCCCATTCTGGGTTATAAGAGCAACAAGCTATGATCTCTGTGAGTACTTGAAGTTATCAGATGTGAATTCGAGATTAATATACTATGTTTTCAACACGATGTTGCTGATGTTGCTTGTGTTCCACATATACTGGTGGTTACTCATATGCTCTATGATTAGTAGACAGCTGAAAAACAGGGGAAAAGTTGGAGAAGATATAAGATCTGGTATGTAA

Coding sequence (CDS)

ATGGACTCGATATGGAGCCACAATAGCCCCCCAGCCGCGTTACATTTCCTTATCGCCCTCCACTACGCATTCGCCTTCTTCGCCGCTAGGTTCTTACTCGACAGATTCATTTTTCGTAGGTTGGCCATTTGGCTGTTGAGCAAAGGATCTGCCCCACTGAAACTTGACGAGGCTACCCAGTCAAAGGTTGTAAAATGTTCAGAATCAATGTGGAAACTAGCATACTATGGCACTGTTGAAATATGCATTCTAAAAATTGCATACAATGAACCATGGTTTAGAGATTCAAATCAGTACTTCAAAGGCTGGCCGAATCAGGAGTTGCAACTTCCTCTAAAACTGCTCTATATGTGCCAATGTGGGTTTTACTTATATAGCATCGCTGCGCTTCTTATATGGGAGACTCGGAGAAAGGATTTCTCCGTCATGATGTCTCATCATGTAATTACTGTCATCTTGATCGGATACTCATATATTACAAGATTTTTCCAGATTGGATCGGTTATTCTTGCGCTCCATGATGCAAGTGATGTCTTCATGGAAGCTGCCAAGGTTTTTAAATATTCTGAGAAAGAACTTGGAGCAAGTATTTTCTTTGGATTTTTTGCCATTTCATGGCTCATATTGCGGCTTATATTCTTCCCATTCTGGGTTATAAGAGCAACAAGCTATGATCTCTGTGAGTACTTGAAGTTATCAGATGTGAATTCGAGATTAATATACTATGTTTTCAACACGATGTTGCTGATGTTGCTTGTGTTCCACATATACTGGTGGTTACTCATATGCTCTATGATTAGTAGACAGCTGAAAAACAGGGGAAAAGTTGGAGAAGATATAAGATCTGGTATGTAA

Protein sequence

MDSIWSHNSPPAALHFLIALHYAFAFFAARFLLDRFIFRRLAIWLLSKGSAPLKLDEATQSKVVKCSESMWKLAYYGTVEICILKIAYNEPWFRDSNQYFKGWPNQELQLPLKLLYMCQCGFYLYSIAALLIWETRRKDFSVMMSHHVITVILIGYSYITRFFQIGSVILALHDASDVFMEAAKVFKYSEKELGASIFFGFFAISWLILRLIFFPFWVIRATSYDLCEYLKLSDVNSRLIYYVFNTMLLMLLVFHIYWWLLICSMISRQLKNRGKVGEDIRSGM

Homology

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BLAST of Lsi03G004910 vs. Swiss-Prot
Match: LAG12_ARATH (LAG1 longevity assurance homolog 2 OS=Arabidopsis thaliana GN=LAG2 PE=1 SV=1)

HSP 1 Score: 391.3 bits (1004), Expect = 8.7e-108
Identity = 187/289 (64.71%), Postives = 231/289 (79.93%), Query Frame = 1


Query: 1   MDSIWSHNSPPAAL------HFLIALHYAFAFFAARFLLDRFIFRRLAIWLLSKGSAPLK 60
           M+S+ S    P         HF IA+++AF FF  R +LDR++F+R+A+WLLS GSAP+K
Sbjct: 1   MESVSSRGGDPVVKPSMEVWHFQIAVYFAFGFFFLRLVLDRYVFQRIALWLLSTGSAPIK 60

Query: 61  L-DEATQSKVVKCSESMWKLAYYGTVEICILKIAYNEPWFRDSNQYFKGWPNQELQLPLK 120
           L D AT++K+VKC ES+WKL YY   +  +L++ Y+EPW RD   YF GWPNQEL+L +K
Sbjct: 61  LNDAATRAKIVKCKESLWKLLYYAACDFFVLQVIYHEPWARDIKLYFHGWPNQELKLSIK 120

Query: 121 LLYMCQCGFYLYSIAALLIWETRRKDFSVMMSHHVITVILIGYSYITRFFQIGSVILALH 180
           L YMCQCGFY+Y +AALL WETRRKDF+VMMSHHVIT+IL+ YSY+T FF+IG++ILALH
Sbjct: 121 LYYMCQCGFYVYGVAALLAWETRRKDFAVMMSHHVITIILLSYSYLTSFFRIGAIILALH 180

Query: 181 DASDVFMEAAKVFKYSEKELGASIFFGFFAISWLILRLIFFPFWVIRATSYDLCEYLKLS 240
           DASDVFME AK+FKYSEKE GAS+ F  FA+SWL+LRLI+FPFW+IRATS +L +YL ++
Sbjct: 181 DASDVFMETAKIFKYSEKEFGASVCFALFAVSWLLLRLIYFPFWIIRATSIELLDYLDMT 240

Query: 241 DVNSRLIYYVFNTMLLMLLVFHIYWWLLICSMISRQLKNRGKVGEDIRS 283
                L+YY FNTMLLMLLVFHIYWW LIC+MI R LKNRGKVGEDIRS
Sbjct: 241 SAEGTLMYYSFNTMLLMLLVFHIYWWYLICAMIVRLLKNRGKVGEDIRS 289

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BLAST of Lsi03G004910 vs. Swiss-Prot
Match: ASCL3_ORYSJ (ASC1-like protein 3 OS=Oryza sativa subsp. japonica GN=Os03g0264000 PE=2 SV=1)

HSP 1 Score: 336.7 bits (862), Expect = 2.5e-91
Identity = 163/273 (59.71%), Postives = 209/273 (76.56%), Query Frame = 1


Query: 10  PPAALHFLIALHYAFAFFAARFLLDRFIFRRLAIWLLSKGSAPLKLDEATQSKVVKCSES 69
           P A+  F + L ++  FF ARF LDR +++ LA +  S  ++ L  DE  Q+K+VK SES
Sbjct: 6   PEASSFFPLTLVFSVGFFCARFFLDRLVYKPLAAYCFSSKASKLMNDEVRQAKIVKFSES 65

Query: 70  MWKLAYYGTVEICILKIAYNEPWFRDSNQYFKGWPNQELQLPLKLLYMCQCGFYLYSIAA 129
           +WKL YYG+V+  +L I   EPW  D+ QYF+GWPNQ +   L L YMCQCGFY+YSI A
Sbjct: 66  IWKLTYYGSVQAWVLLIIKQEPWSLDTMQYFEGWPNQYMTSSLMLFYMCQCGFYIYSIFA 125

Query: 130 LLIWETRRKDFSVMMSHHVITVILIGYSYITRFFQIGSVILALHDASDVFMEAAKVFKYS 189
           L+ WETRRKDF+VMMSHHV+T ILIGY+Y+T FF+IG++ILALHDASDVF+E AK+ KY+
Sbjct: 126 LVAWETRRKDFAVMMSHHVVTSILIGYAYLTGFFRIGTIILALHDASDVFLETAKLCKYT 185

Query: 190 EKELGASIFFGFFAISWLILRLIFFPFWVIRATSYDLCEYLKLSDVNSRLIYYVFNTMLL 249
           EKELGAS+FFG FA+SWL+LRLI+FPFW+I+ +SY     L+  +     +YY+FNTMLL
Sbjct: 186 EKELGASLFFGLFALSWLLLRLIYFPFWIIKTSSYQSIISLRKLEKFPTTLYYIFNTMLL 245

Query: 250 MLLVFHIYWWLLICSMISRQLKNRGKVGEDIRS 283
            LLVFHIYWW LIC MI +QL N+G+VGED+RS
Sbjct: 246 TLLVFHIYWWKLICLMIMKQLNNKGQVGEDVRS 278

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BLAST of Lsi03G004910 vs. Swiss-Prot
Match: LAG13_ARATH (LAG1 longevity assurance homolog 3 OS=Arabidopsis thaliana GN=At1g13580 PE=2 SV=2)

HSP 1 Score: 255.0 bits (650), Expect = 9.8e-67
Identity = 135/287 (47.04%), Postives = 185/287 (64.46%), Query Frame = 1


Query: 5   WSHNSPPAALHFLIALHYAFAFFAARFLLDRFIFRRLAIWLLSKGSAPLKLDEATQ--SK 64
           W H S P    F +   +A  F + RFLLDRF+F +LA +L+         D+ T+   K
Sbjct: 12  WEHESSPVYQDFRVLPLFAVFFPSIRFLLDRFVFEKLAKYLIYGKHRQDMGDDTTERKKK 71

Query: 65  VVKCSESMWKLAYYGTVEICILKIAYNEPWFRDSNQYFKG-----WPNQELQLPLKLLYM 124
           + K  ES WK  YY + EI  L + YNEPWF ++  ++ G     WP+Q+ +L LKLLYM
Sbjct: 72  IRKFKESAWKCVYYLSAEILALSVTYNEPWFMNTKYFWVGPGDQTWPDQQTKLKLKLLYM 131

Query: 125 CQCGFYLYSIAALLIWETRRKDFSVMMSHHVITVILIGYSYITRFFQIGSVILALHDASD 184
              GFY YSI AL+ WETRR DF V M HH+ T+ILI  SY+  F ++GSV+LALHDASD
Sbjct: 132 FVAGFYTYSIFALVFWETRRSDFGVSMGHHIATLILIVLSYVCSFSRVGSVVLALHDASD 191

Query: 185 VFMEAAKVFKYSEKELGASIFFGFFAISWLILRLIFFPFWVIRATSYDLCEYLKLS--DV 244
           VF+E  K+ KYS  E  AS  F  F +SW+ILRLI++PFW++ +TSY++   L      +
Sbjct: 192 VFLEVGKMSKYSGAERIASFSFILFVLSWIILRLIYYPFWILWSTSYEVVLELDKDKHPI 251

Query: 245 NSRLIYYVFNTMLLMLLVFHIYWWLLICSMISRQLKNRGKVGEDIRS 283
              + YY+FNT+L  LLV HIYWW+L+  M+ +Q+++RGK+ ED+RS
Sbjct: 252 EGPIYYYMFNTLLYCLLVLHIYWWVLMYRMLVKQIQDRGKLSEDVRS 298

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BLAST of Lsi03G004910 vs. Swiss-Prot
Match: ASCL1_ORYSJ (ASC1-like protein 1 OS=Oryza sativa subsp. japonica GN=Os02g0581300 PE=2 SV=1)

HSP 1 Score: 240.7 bits (613), Expect = 1.9e-62
Identity = 130/285 (45.61%), Postives = 180/285 (63.16%), Query Frame = 1


Query: 5   WSHNSPPAALHFLIALHYAFAFFAARFLLDRFIFRRLAIWLLSKGSAPLKLDEATQSKVV 64
           W   + PA   F     +A  F   R+LLD F+F  +   L+          E T+ K+ 
Sbjct: 16  WEREAYPAYRDFFALPLFAVFFLVVRYLLDCFVFEWIGRKLIFGKEKVDYEKEETRKKIR 75

Query: 65  KCSESMWKLAYYGTVEICILKIAYNEPWFRDSNQYFKG-----WPNQELQLPLKLLYMCQ 124
           K  ES WK  Y+ + EI  L + YNEPWF ++  ++ G     WP+Q+++  LK +YM  
Sbjct: 76  KFKESAWKCVYFLSGEILSLSVTYNEPWFTNTKYFWVGPGDQVWPDQKIKWKLKAVYMYA 135

Query: 125 CGFYLYSIAALLIWETRRKDFSVMMSHHVITVILIGYSYITRFFQIGSVILALHDASDVF 184
            GFY YSI AL+ WETRR DF V MSHHV TV LI  SY+ RF ++GSV+LA+HDASDVF
Sbjct: 136 AGFYTYSIFALMFWETRRSDFGVSMSHHVATVALIVLSYVFRFARVGSVVLAIHDASDVF 195

Query: 185 MEAAKVFKYSEKELGASIFFGFFAISWLILRLIFFPFWVIRATSYDLCEYL--KLSDVNS 244
           +E  K+ KYS  +L A++ F  F +SW++LRL +FPFW++R+TSY++   L  K  + + 
Sbjct: 196 LEVGKMAKYSHCDLLANVAFLLFVVSWVLLRLTYFPFWILRSTSYEVLLTLDKKKHNFDG 255

Query: 245 RLIYYVFNTMLLMLLVFHIYWWLLICSMISRQLKNRGKVGEDIRS 283
            + YYVFN++L  LLV HIYWW+LI  M+ RQ+K R  VG+D+RS
Sbjct: 256 PIYYYVFNSLLFSLLVLHIYWWVLIYRMLVRQIKTR-NVGDDVRS 299

[Close]

BLAST of Lsi03G004910 vs. Swiss-Prot
Match: LAG11_ARATH (LAG1 longevity assurance homolog 1 OS=Arabidopsis thaliana GN=LAG1 PE=2 SV=1)

HSP 1 Score: 240.7 bits (613), Expect = 1.9e-62
Identity = 132/289 (45.67%), Postives = 181/289 (62.63%), Query Frame = 1


Query: 5   WSHNSPPAALHFLIALHYAFAFFAARFLLDRFIFRRLAIWLL-----SKGSAPLKLDEAT 64
           W   S P          +A  F   RFLLDRF+F +LA  ++     +  S  +K  +  
Sbjct: 12  WEQESFPTYQDLGFLPLFAVFFPTIRFLLDRFVFEKLASLVIYGRMSTNKSDNIKDRKKN 71

Query: 65  QSKVVKCSESMWKLAYYGTVEICILKIAYNEPWFRDSNQYFKG-----WPNQELQLPLKL 124
             KV K  ES WK  YY + E+  L + YNEPWF ++  ++ G     WP+Q +++ LK 
Sbjct: 72  SPKVRKFKESAWKCIYYLSAELLALSVTYNEPWFSNTLYFWIGPGDQIWPDQPMKMKLKF 131

Query: 125 LYMCQCGFYLYSIAALLIWETRRKDFSVMMSHHVITVILIGYSYITRFFQIGSVILALHD 184
           LYM   GFY YSI AL+ WETRR DF V M HH+ T++LI  SYI R  + GSVILALHD
Sbjct: 132 LYMFAAGFYTYSIFALVFWETRRSDFGVSMGHHITTLVLIVLSYICRLTRAGSVILALHD 191

Query: 185 ASDVFMEAAKVFKYSEKELGASIFFGFFAISWLILRLIFFPFWVIRATSYDLCEYL-KLS 244
           ASDVF+E  K+ KY   E  ASI F  FA+SW++LRLI++PFW++ +TSY +   + K  
Sbjct: 192 ASDVFLEIGKMSKYCGAESLASISFVLFALSWVVLRLIYYPFWILWSTSYQIIMTVDKEK 251

Query: 245 DVNSRLIYYVFNTMLLMLLVFHIYWWLLICSMISRQLKNRGKVGEDIRS 283
             N  ++YY+FNT+L  LLV HI+WW+LI  M+ +Q+++RGK+ ED+RS
Sbjct: 252 HPNGPILYYMFNTLLYFLLVLHIFWWVLIYRMLVKQVQDRGKLSEDVRS 300

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BLAST of Lsi03G004910 vs. TrEMBL
Match: A0A0A0K373_CUCSA (Uncharacterized protein OS=Cucumis sativus GN=Csa_7G073690 PE=4 SV=1)

HSP 1 Score: 543.5 bits (1399), Expect = 1.5e-151
Identity = 268/281 (95.37%), Postives = 275/281 (97.86%), Query Frame = 1


Query: 2   DSIWSHNSPPAALHFLIALHYAFAFFAARFLLDRFIFRRLAIWLLSKGSAPLKLDEATQS 61
           DSIW+H SPP  LHF IALH++FAFFAARFLLDRFIFRR AIWLLSKGSAPLKLDEATQS
Sbjct: 3   DSIWTHTSPPHLLHFFIALHFSFAFFAARFLLDRFIFRRFAIWLLSKGSAPLKLDEATQS 62

Query: 62  KVVKCSESMWKLAYYGTVEICILKIAYNEPWFRDSNQYFKGWPNQELQLPLKLLYMCQCG 121
           KVVKCSESMWKLAYYGTVEICILKIAYNEPWFRDSNQYFKGWPNQELQLPLKLLYMCQCG
Sbjct: 63  KVVKCSESMWKLAYYGTVEICILKIAYNEPWFRDSNQYFKGWPNQELQLPLKLLYMCQCG 122

Query: 122 FYLYSIAALLIWETRRKDFSVMMSHHVITVILIGYSYITRFFQIGSVILALHDASDVFME 181
           FYLYSIAALLIWETRRKDFSVMMSHHVITVILIGYSY+TRFFQIGSVILALHDASDVFME
Sbjct: 123 FYLYSIAALLIWETRRKDFSVMMSHHVITVILIGYSYMTRFFQIGSVILALHDASDVFME 182

Query: 182 AAKVFKYSEKELGASIFFGFFAISWLILRLIFFPFWVIRATSYDLCEYLKLSDVNSRLIY 241
           AAKVFKYSEKELGAS+FFGFFAISWL+LRLIFFPFWVI+ATSYDLC YLKLSDVNSRLIY
Sbjct: 183 AAKVFKYSEKELGASVFFGFFAISWLVLRLIFFPFWVIKATSYDLCAYLKLSDVNSRLIY 242

Query: 242 YVFNTMLLMLLVFHIYWWLLICSMISRQLKNRGKVGEDIRS 283
           YVFNTMLLMLLVFHIYWWLLICSMISRQLKNRGKVGEDIRS
Sbjct: 243 YVFNTMLLMLLVFHIYWWLLICSMISRQLKNRGKVGEDIRS 283

[Close]

BLAST of Lsi03G004910 vs. TrEMBL
Match: A0A061E0W3_THECC (Longevity assurance factor, putative OS=Theobroma cacao GN=TCM_007062 PE=4 SV=1)

HSP 1 Score: 464.2 bits (1193), Expect = 1.2e-127
Identity = 220/279 (78.85%), Postives = 253/279 (90.68%), Query Frame = 1


Query: 4   IWSHNSPPAALHFLIALHYAFAFFAARFLLDRFIFRRLAIWLLSKGSAPLKLDEATQSKV 63
           IWS N+ P ALHFL+A+++AF F AARF LD+FIFRRLAIWLLSKGS PLK++EATQ+K+
Sbjct: 5   IWSRNAKPDALHFLLAVYFAFGFVAARFFLDKFIFRRLAIWLLSKGSGPLKMNEATQAKI 64

Query: 64  VKCSESMWKLAYYGTVEICILKIAYNEPWFRDSNQYFKGWPNQELQLPLKLLYMCQCGFY 123
           VKCSESMWKLAYY TVE C+L+I Y EPWFRD+ +YF+GWP+QEL LPL L YMCQCGFY
Sbjct: 65  VKCSESMWKLAYYATVETCVLRITYYEPWFRDTKEYFRGWPDQELNLPLSLYYMCQCGFY 124

Query: 124 LYSIAALLIWETRRKDFSVMMSHHVITVILIGYSYITRFFQIGSVILALHDASDVFMEAA 183
           +YSIAALL WETRRKDF+VMMSHH+ITVILIGYSY+TRFF+IG++ILALHDASDVFMEAA
Sbjct: 125 IYSIAALLTWETRRKDFAVMMSHHIITVILIGYSYVTRFFRIGTIILALHDASDVFMEAA 184

Query: 184 KVFKYSEKELGASIFFGFFAISWLILRLIFFPFWVIRATSYDLCEYLKLSDVNSRLIYYV 243
           KVFKYSE+ELGAS+FFG FAISWL+LRLIFFPFWVIR +SYD+ EYL LS+   R +YYV
Sbjct: 185 KVFKYSERELGASVFFGLFAISWLLLRLIFFPFWVIRTSSYDVREYLNLSESYPRFLYYV 244

Query: 244 FNTMLLMLLVFHIYWWLLICSMISRQLKNRGKVGEDIRS 283
            NT+LLMLLVFH+YWW+LICSMISRQL NRGKVGEDIRS
Sbjct: 245 CNTLLLMLLVFHVYWWVLICSMISRQLNNRGKVGEDIRS 283

[Close]

BLAST of Lsi03G004910 vs. TrEMBL
Match: M5VXQ9_PRUPE (Uncharacterized protein OS=Prunus persica GN=PRUPE_ppa009536mg PE=4 SV=1)

HSP 1 Score: 453.4 bits (1165), Expect = 2.1e-124
Identity = 219/282 (77.66%), Postives = 247/282 (87.59%), Query Frame = 1


Query: 1   MDSIWSHNSPPAALHFLIALHYAFAFFAARFLLDRFIFRRLAIWLLSKGSAPLKLDEATQ 60
           M+S WSHN  P A HFL+ +++AF F AAR   DRFIF ++AIWLLSKGSAPLKLD AT+
Sbjct: 1   MESFWSHNGAPDAWHFLVVIYFAFGFLAARLFFDRFIFCKIAIWLLSKGSAPLKLDMATR 60

Query: 61  SKVVKCSESMWKLAYYGTVEICILKIAYNEPWFRDSNQYFKGWPNQELQLPLKLLYMCQC 120
           +KVVKCSES+WKLAYY TVE CILKI+Y EPWFRD+ +YFKGWPNQEL LPLKLLYM QC
Sbjct: 61  AKVVKCSESLWKLAYYATVEACILKISYQEPWFRDTKEYFKGWPNQELGLPLKLLYMGQC 120

Query: 121 GFYLYSIAALLIWETRRKDFSVMMSHHVITVILIGYSYITRFFQIGSVILALHDASDVFM 180
           GFY YSI ALL WETRRKDFSVMM HHVITV+LIG+SYIT FF+IGS+ILALHDASDVF+
Sbjct: 121 GFYTYSIVALLTWETRRKDFSVMMGHHVITVLLIGFSYITSFFRIGSIILALHDASDVFL 180

Query: 181 EAAKVFKYSEKELGASIFFGFFAISWLILRLIFFPFWVIRATSYDLCEYLKLSDVNSRLI 240
           EAAKVFKYSEKEL AS+ FGFFA+SWLILRLIFFPFWVIR++SYDL + L LS   SR +
Sbjct: 181 EAAKVFKYSEKELAASVLFGFFAVSWLILRLIFFPFWVIRSSSYDLIDCLDLSKPASRAL 240

Query: 241 YYVFNTMLLMLLVFHIYWWLLICSMISRQLKNRGKVGEDIRS 283
           YYVFNTMLLML VFH+YWW++IC+MI RQLKNRGKVGEDIRS
Sbjct: 241 YYVFNTMLLMLFVFHLYWWVMICAMIRRQLKNRGKVGEDIRS 282

[Close]

BLAST of Lsi03G004910 vs. TrEMBL
Match: H6WCP6_GOSHI (Longevity assurance protein 1-like protein OS=Gossypium hirsutum PE=2 SV=1)

HSP 1 Score: 442.2 bits (1136), Expect = 4.8e-121
Identity = 214/280 (76.43%), Postives = 242/280 (86.43%), Query Frame = 1


Query: 3   SIWSHNSPPAALHFLIALHYAFAFFAARFLLDRFIFRRLAIWLLSKGSAPLKLDEATQSK 62
           SIWS N+ P   HFL+A+H+A  F  ARF LD+FIFRRLAIWL S G APLK++EATQ+K
Sbjct: 4   SIWSRNAKPDVCHFLVAVHFALGFVVARFFLDKFIFRRLAIWLSSNGYAPLKMNEATQAK 63

Query: 63  VVKCSESMWKLAYYGTVEICILKIAYNEPWFRDSNQYFKGWPNQELQLPLKLLYMCQCGF 122
           + KCSESMWKLAYY TVE  ILKI Y+EPWF D+ QYF+GWP+QEL+L L L YMCQCGF
Sbjct: 64  IAKCSESMWKLAYYATVETFILKITYHEPWFTDTKQYFRGWPDQELKLSLSLFYMCQCGF 123

Query: 123 YLYSIAALLIWETRRKDFSVMMSHHVITVILIGYSYITRFFQIGSVILALHDASDVFMEA 182
           Y+YSIAALL WETRRKDF+VMMSHHVITVILIGYSYIT FF+IGS+ILALHDASDVF+EA
Sbjct: 124 YIYSIAALLTWETRRKDFAVMMSHHVITVILIGYSYITSFFRIGSIILALHDASDVFLEA 183

Query: 183 AKVFKYSEKELGASIFFGFFAISWLILRLIFFPFWVIRATSYDLCEYLKLSDVNSRLIYY 242
           AKVFKYSE ELGAS+ FG FAISWL+LRLIFFPFWVI+ +SYD+ E+L LS+     +YY
Sbjct: 184 AKVFKYSESELGASVCFGLFAISWLLLRLIFFPFWVIKTSSYDVREFLNLSESYPMSLYY 243

Query: 243 VFNTMLLMLLVFHIYWWLLICSMISRQLKNRGKVGEDIRS 283
           VFNTMLLMLLVFH+YWW LICSMI RQLKNRGKVGEDIRS
Sbjct: 244 VFNTMLLMLLVFHVYWWFLICSMIMRQLKNRGKVGEDIRS 283

[Close]

BLAST of Lsi03G004910 vs. TrEMBL
Match: A0A022RP89_ERYGU (Uncharacterized protein OS=Erythranthe guttata GN=MIMGU_mgv1a011272mg PE=4 SV=1)

HSP 1 Score: 440.3 bits (1131), Expect = 1.8e-120
Identity = 208/282 (73.76%), Postives = 246/282 (87.23%), Query Frame = 1


Query: 1   MDSIWSHNSPPAALHFLIALHYAFAFFAARFLLDRFIFRRLAIWLLSKGSAPLKLDEATQ 60
           M+SIW+HN  P A HF+ A+++AFAF A RF+LDRFIFRRLAIWLLS+G+A LKL++ T+
Sbjct: 1   MESIWTHNGVPTASHFIFAVYFAFAFVAVRFILDRFIFRRLAIWLLSRGTAQLKLNDETR 60

Query: 61  SKVVKCSESMWKLAYYGTVEICILKIAYNEPWFRDSNQYFKGWPNQELQLPLKLLYMCQC 120
           +K+ KC+ESMWKL YY TVE CIL   Y EPWF D  +YF GWP+QEL+LPLKL+YMCQC
Sbjct: 61  AKIAKCTESMWKLTYYATVEFCILATIYQEPWFIDIKKYFTGWPDQELKLPLKLIYMCQC 120

Query: 121 GFYLYSIAALLIWETRRKDFSVMMSHHVITVILIGYSYITRFFQIGSVILALHDASDVFM 180
           GFY YSIAALL WETRRKDFSVMMSHH++TVILI  SY+TRFF+IG+VILALHDASDVF+
Sbjct: 121 GFYTYSIAALLAWETRRKDFSVMMSHHIVTVILISTSYVTRFFRIGAVILALHDASDVFL 180

Query: 181 EAAKVFKYSEKELGASIFFGFFAISWLILRLIFFPFWVIRATSYDLCEYLKLSDVNSRLI 240
           EAAKVFKYS KE GAS+FFG FA+SW++LRLIFFPFWVIR++SY LCE L LS+  S  +
Sbjct: 181 EAAKVFKYSGKEFGASLFFGLFALSWVLLRLIFFPFWVIRSSSYYLCEVLNLSETYSATL 240

Query: 241 YYVFNTMLLMLLVFHIYWWLLICSMISRQLKNRGKVGEDIRS 283
           YY+FNTMLL LL+FHIYWW+LICSMI+RQLKNRG+VGEDIRS
Sbjct: 241 YYLFNTMLLTLLIFHIYWWILICSMITRQLKNRGQVGEDIRS 282

[Close]

BLAST of Lsi03G004910 vs. TAIR10
Match: AT3G19260.1 (AT3G19260.1 LAG1 homologue 2)

HSP 1 Score: 391.3 bits (1004), Expect = 4.9e-109
Identity = 187/289 (64.71%), Postives = 231/289 (79.93%), Query Frame = 1


Query: 1   MDSIWSHNSPPAAL------HFLIALHYAFAFFAARFLLDRFIFRRLAIWLLSKGSAPLK 60
           M+S+ S    P         HF IA+++AF FF  R +LDR++F+R+A+WLLS GSAP+K
Sbjct: 1   MESVSSRGGDPVVKPSMEVWHFQIAVYFAFGFFFLRLVLDRYVFQRIALWLLSTGSAPIK 60

Query: 61  L-DEATQSKVVKCSESMWKLAYYGTVEICILKIAYNEPWFRDSNQYFKGWPNQELQLPLK 120
           L D AT++K+VKC ES+WKL YY   +  +L++ Y+EPW RD   YF GWPNQEL+L +K
Sbjct: 61  LNDAATRAKIVKCKESLWKLLYYAACDFFVLQVIYHEPWARDIKLYFHGWPNQELKLSIK 120

Query: 121 LLYMCQCGFYLYSIAALLIWETRRKDFSVMMSHHVITVILIGYSYITRFFQIGSVILALH 180
           L YMCQCGFY+Y +AALL WETRRKDF+VMMSHHVIT+IL+ YSY+T FF+IG++ILALH
Sbjct: 121 LYYMCQCGFYVYGVAALLAWETRRKDFAVMMSHHVITIILLSYSYLTSFFRIGAIILALH 180

Query: 181 DASDVFMEAAKVFKYSEKELGASIFFGFFAISWLILRLIFFPFWVIRATSYDLCEYLKLS 240
           DASDVFME AK+FKYSEKE GAS+ F  FA+SWL+LRLI+FPFW+IRATS +L +YL ++
Sbjct: 181 DASDVFMETAKIFKYSEKEFGASVCFALFAVSWLLLRLIYFPFWIIRATSIELLDYLDMT 240

Query: 241 DVNSRLIYYVFNTMLLMLLVFHIYWWLLICSMISRQLKNRGKVGEDIRS 283
                L+YY FNTMLLMLLVFHIYWW LIC+MI R LKNRGKVGEDIRS
Sbjct: 241 SAEGTLMYYSFNTMLLMLLVFHIYWWYLICAMIVRLLKNRGKVGEDIRS 289

[Close]

BLAST of Lsi03G004910 vs. TAIR10
Match: AT1G13580.2 (AT1G13580.2 LAG1 longevity assurance homolog 3)

HSP 1 Score: 255.0 bits (650), Expect = 5.5e-68
Identity = 135/287 (47.04%), Postives = 185/287 (64.46%), Query Frame = 1


Query: 5   WSHNSPPAALHFLIALHYAFAFFAARFLLDRFIFRRLAIWLLSKGSAPLKLDEATQ--SK 64
           W H S P    F +   +A  F + RFLLDRF+F +LA +L+         D+ T+   K
Sbjct: 12  WEHESSPVYQDFRVLPLFAVFFPSIRFLLDRFVFEKLAKYLIYGKHRQDMGDDTTERKKK 71

Query: 65  VVKCSESMWKLAYYGTVEICILKIAYNEPWFRDSNQYFKG-----WPNQELQLPLKLLYM 124
           + K  ES WK  YY + EI  L + YNEPWF ++  ++ G     WP+Q+ +L LKLLYM
Sbjct: 72  IRKFKESAWKCVYYLSAEILALSVTYNEPWFMNTKYFWVGPGDQTWPDQQTKLKLKLLYM 131

Query: 125 CQCGFYLYSIAALLIWETRRKDFSVMMSHHVITVILIGYSYITRFFQIGSVILALHDASD 184
              GFY YSI AL+ WETRR DF V M HH+ T+ILI  SY+  F ++GSV+LALHDASD
Sbjct: 132 FVAGFYTYSIFALVFWETRRSDFGVSMGHHIATLILIVLSYVCSFSRVGSVVLALHDASD 191

Query: 185 VFMEAAKVFKYSEKELGASIFFGFFAISWLILRLIFFPFWVIRATSYDLCEYLKLS--DV 244
           VF+E  K+ KYS  E  AS  F  F +SW+ILRLI++PFW++ +TSY++   L      +
Sbjct: 192 VFLEVGKMSKYSGAERIASFSFILFVLSWIILRLIYYPFWILWSTSYEVVLELDKDKHPI 251

Query: 245 NSRLIYYVFNTMLLMLLVFHIYWWLLICSMISRQLKNRGKVGEDIRS 283
              + YY+FNT+L  LLV HIYWW+L+  M+ +Q+++RGK+ ED+RS
Sbjct: 252 EGPIYYYMFNTLLYCLLVLHIYWWVLMYRMLVKQIQDRGKLSEDVRS 298

[Close]

BLAST of Lsi03G004910 vs. TAIR10
Match: AT3G25540.1 (AT3G25540.1 TRAM, LAG1 and CLN8 (TLC) lipid-sensing domain containing protein)

HSP 1 Score: 240.7 bits (613), Expect = 1.1e-63
Identity = 132/289 (45.67%), Postives = 181/289 (62.63%), Query Frame = 1


Query: 5   WSHNSPPAALHFLIALHYAFAFFAARFLLDRFIFRRLAIWLL-----SKGSAPLKLDEAT 64
           W   S P          +A  F   RFLLDRF+F +LA  ++     +  S  +K  +  
Sbjct: 12  WEQESFPTYQDLGFLPLFAVFFPTIRFLLDRFVFEKLASLVIYGRMSTNKSDNIKDRKKN 71

Query: 65  QSKVVKCSESMWKLAYYGTVEICILKIAYNEPWFRDSNQYFKG-----WPNQELQLPLKL 124
             KV K  ES WK  YY + E+  L + YNEPWF ++  ++ G     WP+Q +++ LK 
Sbjct: 72  SPKVRKFKESAWKCIYYLSAELLALSVTYNEPWFSNTLYFWIGPGDQIWPDQPMKMKLKF 131

Query: 125 LYMCQCGFYLYSIAALLIWETRRKDFSVMMSHHVITVILIGYSYITRFFQIGSVILALHD 184
           LYM   GFY YSI AL+ WETRR DF V M HH+ T++LI  SYI R  + GSVILALHD
Sbjct: 132 LYMFAAGFYTYSIFALVFWETRRSDFGVSMGHHITTLVLIVLSYICRLTRAGSVILALHD 191

Query: 185 ASDVFMEAAKVFKYSEKELGASIFFGFFAISWLILRLIFFPFWVIRATSYDLCEYL-KLS 244
           ASDVF+E  K+ KY   E  ASI F  FA+SW++LRLI++PFW++ +TSY +   + K  
Sbjct: 192 ASDVFLEIGKMSKYCGAESLASISFVLFALSWVVLRLIYYPFWILWSTSYQIIMTVDKEK 251

Query: 245 DVNSRLIYYVFNTMLLMLLVFHIYWWLLICSMISRQLKNRGKVGEDIRS 283
             N  ++YY+FNT+L  LLV HI+WW+LI  M+ +Q+++RGK+ ED+RS
Sbjct: 252 HPNGPILYYMFNTLLYFLLVLHIFWWVLIYRMLVKQVQDRGKLSEDVRS 300

[Close]

BLAST of Lsi03G004910 vs. TAIR10
Match: AT1G26200.1 (AT1G26200.1 TRAM, LAG1 and CLN8 (TLC) lipid-sensing domain containing protein)

HSP 1 Score: 215.7 bits (548), Expect = 3.7e-56
Identity = 123/292 (42.12%), Postives = 174/292 (59.59%), Query Frame = 1


Query: 5   WSHNSPPAALHFLIALHYAFAFFAARFLLDRFIFRRLAIWLLSKGSAPLKLDEATQSKVV 64
           W   S P +  FL+ + +A  F   R +LDR IF R+A  L+         +E  + KVV
Sbjct: 11  WDQESYPDSSDFLVLIFFAPFFLFLRLILDRCIFERVARRLVVPKGLCADSNER-RKKVV 70

Query: 65  KCSESMWKLAYYGTVEICILKIAYNEPWFRDSNQYFKG-----WPNQELQLPLKLLYMCQ 124
           K  ES WK     +VE   L + Y EPWF+D+  ++ G     WP+Q+++L +K +YM  
Sbjct: 71  KFKESAWKCLCSFSVEAFALYVTYKEPWFKDTRSFWLGPGDQVWPDQKIKLKMKGMYMFV 130

Query: 125 CGFYLYSIAALLIWETRRKDFSVMMSHHVITVILIGYSYITRFFQIGSVILALHDASDVF 184
            G  +Y+  AL  WETRR DF VM+ HH++T  LI  SY+ RF +IGSVILALH+ SDVF
Sbjct: 131 GGLNVYAFFALFFWETRRSDFKVMLVHHIVTSFLIILSYVFRFARIGSVILALHEISDVF 190

Query: 185 MEAAKVFKYSEKELGASIFFGFFAISWLILRLIFFPFWVIRATSYDLC---------EYL 244
           +E  K+ KYS  E   S+ F  F +SW  LRLI++PFW++ +TSY+           ++L
Sbjct: 191 LEIGKMCKYSGAETMTSVSFVLFFLSWTTLRLIYYPFWILWSTSYESIKVKTEYWDKKHL 250

Query: 245 KLSDVNSRLIYYVFNTMLLMLLVFHIYWWLLICSMISRQLKNRGKVGEDIRS 283
             +     L YYVFNT+L  L + HIYWW+LI  ++  Q++ +GKV +DIRS
Sbjct: 251 METGPPLILFYYVFNTLLYCLQILHIYWWILIYRVLISQIRAKGKVAKDIRS 301

[Close]

BLAST of Lsi03G004910 vs. NCBI nr
Match: gi|659109911|ref|XP_008454946.1| (PREDICTED: LAG1 longevity assurance homolog 2 [Cucumis melo])

HSP 1 Score: 545.8 bits (1405), Expect = 4.4e-152
Identity = 269/281 (95.73%), Postives = 276/281 (98.22%), Query Frame = 1


Query: 2   DSIWSHNSPPAALHFLIALHYAFAFFAARFLLDRFIFRRLAIWLLSKGSAPLKLDEATQS 61
           DSIW+H SPP  LHF IALH++FAFFAARFLLDRFIFRR AIWLLSKG+APLKLDEATQS
Sbjct: 3   DSIWTHTSPPHLLHFFIALHFSFAFFAARFLLDRFIFRRFAIWLLSKGAAPLKLDEATQS 62

Query: 62  KVVKCSESMWKLAYYGTVEICILKIAYNEPWFRDSNQYFKGWPNQELQLPLKLLYMCQCG 121
           KVVKCSESMWKLAYYGTVEICILKIAYNEPWFRDSNQYFKGWPNQELQLPLKLLYMCQCG
Sbjct: 63  KVVKCSESMWKLAYYGTVEICILKIAYNEPWFRDSNQYFKGWPNQELQLPLKLLYMCQCG 122

Query: 122 FYLYSIAALLIWETRRKDFSVMMSHHVITVILIGYSYITRFFQIGSVILALHDASDVFME 181
           FYLYSIAALLIWETRRKDFSVMMSHHVITVILIGYSYITRFFQIGSVILALHDASDVFME
Sbjct: 123 FYLYSIAALLIWETRRKDFSVMMSHHVITVILIGYSYITRFFQIGSVILALHDASDVFME 182

Query: 182 AAKVFKYSEKELGASIFFGFFAISWLILRLIFFPFWVIRATSYDLCEYLKLSDVNSRLIY 241
           AAKVFKYSEKELGAS+FFGFFAISWL+LRLIFFPFWVI+ATSYDLCEYLKLSDVNSRLIY
Sbjct: 183 AAKVFKYSEKELGASVFFGFFAISWLVLRLIFFPFWVIKATSYDLCEYLKLSDVNSRLIY 242

Query: 242 YVFNTMLLMLLVFHIYWWLLICSMISRQLKNRGKVGEDIRS 283
           YVFNTMLLMLLVFHIYWWLLICSMISRQLKNRGKVGEDIRS
Sbjct: 243 YVFNTMLLMLLVFHIYWWLLICSMISRQLKNRGKVGEDIRS 283

[Close]

BLAST of Lsi03G004910 vs. NCBI nr
Match: gi|449438412|ref|XP_004136982.1| (PREDICTED: LAG1 longevity assurance homolog 2 [Cucumis sativus])

HSP 1 Score: 543.5 bits (1399), Expect = 2.2e-151
Identity = 268/281 (95.37%), Postives = 275/281 (97.86%), Query Frame = 1


Query: 2   DSIWSHNSPPAALHFLIALHYAFAFFAARFLLDRFIFRRLAIWLLSKGSAPLKLDEATQS 61
           DSIW+H SPP  LHF IALH++FAFFAARFLLDRFIFRR AIWLLSKGSAPLKLDEATQS
Sbjct: 3   DSIWTHTSPPHLLHFFIALHFSFAFFAARFLLDRFIFRRFAIWLLSKGSAPLKLDEATQS 62

Query: 62  KVVKCSESMWKLAYYGTVEICILKIAYNEPWFRDSNQYFKGWPNQELQLPLKLLYMCQCG 121
           KVVKCSESMWKLAYYGTVEICILKIAYNEPWFRDSNQYFKGWPNQELQLPLKLLYMCQCG
Sbjct: 63  KVVKCSESMWKLAYYGTVEICILKIAYNEPWFRDSNQYFKGWPNQELQLPLKLLYMCQCG 122

Query: 122 FYLYSIAALLIWETRRKDFSVMMSHHVITVILIGYSYITRFFQIGSVILALHDASDVFME 181
           FYLYSIAALLIWETRRKDFSVMMSHHVITVILIGYSY+TRFFQIGSVILALHDASDVFME
Sbjct: 123 FYLYSIAALLIWETRRKDFSVMMSHHVITVILIGYSYMTRFFQIGSVILALHDASDVFME 182

Query: 182 AAKVFKYSEKELGASIFFGFFAISWLILRLIFFPFWVIRATSYDLCEYLKLSDVNSRLIY 241
           AAKVFKYSEKELGAS+FFGFFAISWL+LRLIFFPFWVI+ATSYDLC YLKLSDVNSRLIY
Sbjct: 183 AAKVFKYSEKELGASVFFGFFAISWLVLRLIFFPFWVIKATSYDLCAYLKLSDVNSRLIY 242

Query: 242 YVFNTMLLMLLVFHIYWWLLICSMISRQLKNRGKVGEDIRS 283
           YVFNTMLLMLLVFHIYWWLLICSMISRQLKNRGKVGEDIRS
Sbjct: 243 YVFNTMLLMLLVFHIYWWLLICSMISRQLKNRGKVGEDIRS 283

[Close]

BLAST of Lsi03G004910 vs. NCBI nr
Match: gi|719969955|ref|XP_010269982.1| (PREDICTED: LAG1 longevity assurance homolog 2-like [Nelumbo nucifera])

HSP 1 Score: 468.0 bits (1203), Expect = 1.2e-128
Identity = 220/282 (78.01%), Postives = 255/282 (90.43%), Query Frame = 1


Query: 1   MDSIWSHNSPPAALHFLIALHYAFAFFAARFLLDRFIFRRLAIWLLSKGSAPLKLDEATQ 60
           MDSIWSHN  P A HF IA+++A  F AARF LD FI+RRLAIWLLS G+AP+K+ EA Q
Sbjct: 1   MDSIWSHNGVPEAFHFFIAIYFAVGFVAARFFLDTFIYRRLAIWLLSTGTAPVKISEAKQ 60

Query: 61  SKVVKCSESMWKLAYYGTVEICILKIAYNEPWFRDSNQYFKGWPNQELQLPLKLLYMCQC 120
           +K+VKCSESMWKL YY TV++C+LKI YNEPWFRD  +YF+GWPNQE+++PLKLLYMCQC
Sbjct: 61  AKIVKCSESMWKLTYYATVQVCVLKITYNEPWFRDIKEYFRGWPNQEIKVPLKLLYMCQC 120

Query: 121 GFYLYSIAALLIWETRRKDFSVMMSHHVITVILIGYSYITRFFQIGSVILALHDASDVFM 180
           GFY+Y IAALL+WETRRKDFS+MMSHHVITVILIGYSY++RFF+IGS++LALHDASDVFM
Sbjct: 121 GFYVYGIAALLMWETRRKDFSIMMSHHVITVILIGYSYLSRFFRIGSIVLALHDASDVFM 180

Query: 181 EAAKVFKYSEKELGASIFFGFFAISWLILRLIFFPFWVIRATSYDLCEYLKLSDVNSRLI 240
           EAAKVFKYSEKEL AS+FFGFFAISW+ILRLIFFPFWVI+++SYD+ E+L LS+    L+
Sbjct: 181 EAAKVFKYSEKELAASLFFGFFAISWIILRLIFFPFWVIKSSSYDIREFLALSEPYPALL 240

Query: 241 YYVFNTMLLMLLVFHIYWWLLICSMISRQLKNRGKVGEDIRS 283
           YYVFNTMLLMLLVFHIYWW+LICSMI RQLKNRGKVGEDIRS
Sbjct: 241 YYVFNTMLLMLLVFHIYWWILICSMIMRQLKNRGKVGEDIRS 282

[Close]

BLAST of Lsi03G004910 vs. NCBI nr
Match: gi|590686597|ref|XP_007042426.1| (Longevity assurance factor, putative [Theobroma cacao])

HSP 1 Score: 464.2 bits (1193), Expect = 1.7e-127
Identity = 220/279 (78.85%), Postives = 253/279 (90.68%), Query Frame = 1


Query: 4   IWSHNSPPAALHFLIALHYAFAFFAARFLLDRFIFRRLAIWLLSKGSAPLKLDEATQSKV 63
           IWS N+ P ALHFL+A+++AF F AARF LD+FIFRRLAIWLLSKGS PLK++EATQ+K+
Sbjct: 5   IWSRNAKPDALHFLLAVYFAFGFVAARFFLDKFIFRRLAIWLLSKGSGPLKMNEATQAKI 64

Query: 64  VKCSESMWKLAYYGTVEICILKIAYNEPWFRDSNQYFKGWPNQELQLPLKLLYMCQCGFY 123
           VKCSESMWKLAYY TVE C+L+I Y EPWFRD+ +YF+GWP+QEL LPL L YMCQCGFY
Sbjct: 65  VKCSESMWKLAYYATVETCVLRITYYEPWFRDTKEYFRGWPDQELNLPLSLYYMCQCGFY 124

Query: 124 LYSIAALLIWETRRKDFSVMMSHHVITVILIGYSYITRFFQIGSVILALHDASDVFMEAA 183
           +YSIAALL WETRRKDF+VMMSHH+ITVILIGYSY+TRFF+IG++ILALHDASDVFMEAA
Sbjct: 125 IYSIAALLTWETRRKDFAVMMSHHIITVILIGYSYVTRFFRIGTIILALHDASDVFMEAA 184

Query: 184 KVFKYSEKELGASIFFGFFAISWLILRLIFFPFWVIRATSYDLCEYLKLSDVNSRLIYYV 243
           KVFKYSE+ELGAS+FFG FAISWL+LRLIFFPFWVIR +SYD+ EYL LS+   R +YYV
Sbjct: 185 KVFKYSERELGASVFFGLFAISWLLLRLIFFPFWVIRTSSYDVREYLNLSESYPRFLYYV 244

Query: 244 FNTMLLMLLVFHIYWWLLICSMISRQLKNRGKVGEDIRS 283
            NT+LLMLLVFH+YWW+LICSMISRQL NRGKVGEDIRS
Sbjct: 245 CNTLLLMLLVFHVYWWVLICSMISRQLNNRGKVGEDIRS 283

[Close]

BLAST of Lsi03G004910 vs. NCBI nr
Match: gi|720024154|ref|XP_010263552.1| (PREDICTED: LAG1 longevity assurance homolog 2-like [Nelumbo nucifera])

HSP 1 Score: 455.7 bits (1171), Expect = 6.0e-125
Identity = 216/282 (76.60%), Postives = 251/282 (89.01%), Query Frame = 1


Query: 1   MDSIWSHNSPPAALHFLIALHYAFAFFAARFLLDRFIFRRLAIWLLSKGSAPLKLDEATQ 60
           MDSIWSHN  P A HF IA+++AF F AARF LD FI+RRLAIWLL+ G +P+K++EA +
Sbjct: 1   MDSIWSHNGVPDAWHFFIAIYFAFGFVAARFFLDTFIYRRLAIWLLNDGVSPVKINEAKR 60

Query: 61  SKVVKCSESMWKLAYYGTVEICILKIAYNEPWFRDSNQYFKGWPNQELQLPLKLLYMCQC 120
           +K+VKCSESMWKL YY TV+ C+L I Y+EPWFRD+ +YF+GWPNQEL+ PLKLLYMCQC
Sbjct: 61  AKIVKCSESMWKLTYYATVQACVLMIIYHEPWFRDTKEYFRGWPNQELKFPLKLLYMCQC 120

Query: 121 GFYLYSIAALLIWETRRKDFSVMMSHHVITVILIGYSYITRFFQIGSVILALHDASDVFM 180
           GFYLYSIAALL WETRRKDFS+MMSHH+ITVILIGYSY+TRFF+IGS+ILALHDASDVF+
Sbjct: 121 GFYLYSIAALLTWETRRKDFSIMMSHHIITVILIGYSYLTRFFRIGSIILALHDASDVFL 180

Query: 181 EAAKVFKYSEKELGASIFFGFFAISWLILRLIFFPFWVIRATSYDLCEYLKLSDVNSRLI 240
           EAAKVFKYSEKEL AS+ FGFFAISWLILRLIFFPFWVIR +SYD+ E+L LS+   + +
Sbjct: 181 EAAKVFKYSEKELAASLCFGFFAISWLILRLIFFPFWVIRTSSYDVREFLDLSEGYQKSL 240

Query: 241 YYVFNTMLLMLLVFHIYWWLLICSMISRQLKNRGKVGEDIRS 283
           YYVFNTMLL+L +FHIYWW+LICSMI RQLKNRGKVGEDIRS
Sbjct: 241 YYVFNTMLLVLFIFHIYWWILICSMILRQLKNRGKVGEDIRS 282

The following BLAST results are available for this feature:

BLAST of Lsi03G004910 vs. Swiss-Prot


Match Name	E-value	Identity	Description
LAG12_ARATH	8.7e-108	64.71	LAG1 longevity assurance homolog 2 OS=Arabidopsis thaliana GN=LAG2 PE=1 SV=1	[more]
ASCL3_ORYSJ	2.5e-91	59.71	ASC1-like protein 3 OS=Oryza sativa subsp. japonica GN=Os03g0264000 PE=2 SV=1	[more]
LAG13_ARATH	9.8e-67	47.04	LAG1 longevity assurance homolog 3 OS=Arabidopsis thaliana GN=At1g13580 PE=2 SV=...	[more]
ASCL1_ORYSJ	1.9e-62	45.61	ASC1-like protein 1 OS=Oryza sativa subsp. japonica GN=Os02g0581300 PE=2 SV=1	[more]
LAG11_ARATH	1.9e-62	45.67	LAG1 longevity assurance homolog 1 OS=Arabidopsis thaliana GN=LAG1 PE=2 SV=1	[more]

BLAST of Lsi03G004910 vs. TrEMBL


Match Name	E-value	Identity	Description
A0A0A0K373_CUCSA	1.5e-151	95.37	Uncharacterized protein OS=Cucumis sativus GN=Csa_7G073690 PE=4 SV=1	[more]
A0A061E0W3_THECC	1.2e-127	78.85	Longevity assurance factor, putative OS=Theobroma cacao GN=TCM_007062 PE=4 SV=1	[more]
M5VXQ9_PRUPE	2.1e-124	77.66	Uncharacterized protein OS=Prunus persica GN=PRUPE_ppa009536mg PE=4 SV=1	[more]
H6WCP6_GOSHI	4.8e-121	76.43	Longevity assurance protein 1-like protein OS=Gossypium hirsutum PE=2 SV=1	[more]
A0A022RP89_ERYGU	1.8e-120	73.76	Uncharacterized protein OS=Erythranthe guttata GN=MIMGU_mgv1a011272mg PE=4 SV=1	[more]

BLAST of Lsi03G004910 vs. TAIR10


Match Name	E-value	Identity	Description
AT3G19260.1	4.9e-109	64.71	LAG1 homologue 2	[more]
AT1G13580.2	5.5e-68	47.04	LAG1 longevity assurance homolog 3	[more]
AT3G25540.1	1.1e-63	45.67	TRAM, LAG1 and CLN8 (TLC) lipid-sensing domain containing protein	[more]
AT1G26200.1	3.7e-56	42.12	TRAM, LAG1 and CLN8 (TLC) lipid-sensing domain containing protein	[more]

BLAST of Lsi03G004910 vs. NCBI nr


Match Name	E-value	Identity	Description
gi\|659109911\|ref\|XP_008454946.1\|	4.4e-152	95.73	PREDICTED: LAG1 longevity assurance homolog 2 [Cucumis melo]	[more]
gi\|449438412\|ref\|XP_004136982.1\|	2.2e-151	95.37	PREDICTED: LAG1 longevity assurance homolog 2 [Cucumis sativus]	[more]
gi\|719969955\|ref\|XP_010269982.1\|	1.2e-128	78.01	PREDICTED: LAG1 longevity assurance homolog 2-like [Nelumbo nucifera]	[more]
gi\|590686597\|ref\|XP_007042426.1\|	1.7e-127	78.85	Longevity assurance factor, putative [Theobroma cacao]	[more]
gi\|720024154\|ref\|XP_010263552.1\|	6.0e-125	76.60	PREDICTED: LAG1 longevity assurance homolog 2-like [Nelumbo nucifera]	[more]

Annotated Terms

The following terms have been associated with this gene:

Vocabulary: **Cellular Component**
Term	Definition
GO:0005783	endoplasmic reticulum
GO:0016021	integral component of membrane

Vocabulary: **INTERPRO**
Term	Definition
IPR016439	Lag1/Lac1-like
IPR006634	TLC-dom

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0042967	acyl-carrier-protein biosynthetic process
biological_process	GO:0006687	glycosphingolipid metabolic process
biological_process	GO:0042759	long-chain fatty acid biosynthetic process
biological_process	GO:0002238	response to molecule of fungal origin
biological_process	GO:0055114	oxidation-reduction process
biological_process	GO:0008150	biological_process
cellular_component	GO:0005783	endoplasmic reticulum
cellular_component	GO:0005794	Golgi apparatus
cellular_component	GO:0016021	integral component of membrane
cellular_component	GO:0005886	plasma membrane
molecular_function	GO:0050291	sphingosine N-acyltransferase activity
molecular_function	GO:0010295	(+)-abscisic acid 8'-hydroxylase activity
molecular_function	GO:0020037	heme binding
molecular_function	GO:0005506	iron ion binding
molecular_function	GO:0003674	molecular_function

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Lsi03G004910.1	Lsi03G004910.1	mRNA

InterPro

Analysis Name: InterPro Annotations of Lagenaria siceraria
Date Performed: 2017-09-18


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR006634	TRAM/LAG1/CLN8 homology domain	PFAM	PF03798	TRAM_LAG1_CLN8	coord: 65..263 score: 2.6
IPR006634	TRAM/LAG1/CLN8 homology domain	SMART	SM00724	lag1_27	coord: 64..271 score: 9.6
IPR006634	TRAM/LAG1/CLN8 homology domain	PROFILE	PS50922	TLC	coord: 64..271 score: 19
IPR016439	Ceramide synthase component Lag1/Lac1	PIR	PIRSF005225	LAG1_LAC1	coord: 7..284 score: 2.1
IPR016439	Ceramide synthase component Lag1/Lac1	PANTHER	PTHR12560	LONGEVITY ASSURANCE FACTOR 1 LAG1	coord: 1..282 score: 1.9E
None	No IPR available	PANTHER	PTHR12560:SF12	LAG1 LONGEVITY ASSURANCE HOMOLOG 2	coord: 1..282 score: 1.9E

Synteny

The following gene(s) are orthologous to this gene:


Gene	Orthologue	Organism	Block
Lsi03G004910	Csa7G073690	Cucumber (Chinese Long) v2	culsiB522
Lsi03G004910	CSPI07G07660	Wild cucumber (PI 183967)	cpilsiB536
Lsi03G004910	Cucsa.219880	Cucumber (Gy14) v1	cgylsiB367
Lsi03G004910	CmaCh11G017530	Cucurbita maxima (Rimu)	cmalsiB116
Lsi03G004910	CmaCh19G006050	Cucurbita maxima (Rimu)	cmalsiB484
Lsi03G004910	CmoCh19G006230	Cucurbita moschata (Rifu)	cmolsiB468
Lsi03G004910	CmoCh11G013600	Cucurbita moschata (Rifu)	cmolsiB103
Lsi03G004910	Cla016010	Watermelon (97103) v1	lsiwmB268
Lsi03G004910	MELO3C018448	Melon (DHL92) v3.5.1	lsimeB247
Lsi03G004910	ClCG02G005920	Watermelon (Charleston Gray)	lsiwcgB233
Lsi03G004910	MELO3C018448.2	Melon (DHL92) v3.6.1	lsimedB260
Lsi03G004910	Cla97C02G032890	Watermelon (97103) v2	lsiwmbB226
Lsi03G004910	Bhi05G000918	Wax gourd	lsiwgoB316
Lsi03G004910	Cp4.1LG04g08090	Cucurbita pepo (Zucchini)	cpelsiB543
Lsi03G004910	Cp4.1LG15g04950	Cucurbita pepo (Zucchini)	cpelsiB208
Lsi03G004910	CsGy7G007220	Cucumber (Gy14) v2	cgyblsiB488
Lsi03G004910	Carg22065	Silver-seed gourd	carlsiB187
Lsi03G004910	Carg26414	Silver-seed gourd	carlsiB736
Lsi03G004910	CsaV3_7G008620	Cucumber (Chinese Long) v3	cuclsiB555

The following gene(s) are paralogous to this gene:

None

The following block(s) are covering this gene:


Gene	Organism	Block
Lsi03G004910	Wax gourd	lsiwgoB314
Lsi03G004910	Wax gourd	lsiwgoB323
Lsi03G004910	Bottle gourd (USVL1VR-Ls)	lsilsiB132
Lsi03G004910	Cucumber (Gy14) v1	cgylsiB403
Lsi03G004910	Cucurbita maxima (Rimu)	cmalsiB309
Lsi03G004910	Cucurbita maxima (Rimu)	cmalsiB662
Lsi03G004910	Cucurbita moschata (Rifu)	cmolsiB297
Lsi03G004910	Cucurbita moschata (Rifu)	cmolsiB650
Lsi03G004910	Cucurbita pepo (Zucchini)	cpelsiB171
Lsi03G004910	Cucurbita pepo (Zucchini)	cpelsiB348
Lsi03G004910	Cucurbita pepo (Zucchini)	cpelsiB454
Lsi03G004910	Wild cucumber (PI 183967)	cpilsiB188
Lsi03G004910	Cucumber (Chinese Long) v2	culsiB189
Lsi03G004910	Melon (DHL92) v3.5.1	lsimeB267
Lsi03G004910	Watermelon (Charleston Gray)	lsiwcgB242
Lsi03G004910	Watermelon (97103) v1	lsiwmB255
Lsi03G004910	Cucumber (Gy14) v2	cgyblsiB103
Lsi03G004910	Cucumber (Gy14) v2	cgyblsiB173
Lsi03G004910	Melon (DHL92) v3.6.1	lsimedB271
Lsi03G004910	Melon (DHL92) v3.6.1	lsimedB280
Lsi03G004910	Silver-seed gourd	carlsiB342
Lsi03G004910	Cucumber (Chinese Long) v3	cuclsiB205
Lsi03G004910	Watermelon (97103) v2	lsiwmbB236
Lsi03G004910	Watermelon (97103) v2	lsiwmbB244