Lsi03G004450 (gene) Bottle gourd (USVL1VR-Ls)

Overview
NameLsi03G004450
Typegene
OrganismLagenaria siceraria (Bottle gourd (USVL1VR-Ls))
DescriptionProbable tRNA methyltransferase 9-like protein
Locationchr03 : 5066134 .. 5070221 (+)
Genome Viewer
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: five_prime_UTRCDS
Hold the cursor over a type above to highlight its positions in the sequence below.
TTATAATTTAACAAAATAATTAACTCTGCAAAATCAATGTAGAGTCACTAGGGCTTCCTCTTCACTTGACGACGAAGACGACCAACCACTTGAAGCTGCCATTGCGAGATTGGTGCCTTGATCTCCTCTTCTTCTTCTTCTTCTTTCCTCTCTTTTTCTTTCTTTTTCCAATCACGAAACTACATTCCACAATCTCAACCTTCCATTTGTTTTTCTCTAATCTTTCAGTTTAATCATGCAGCCACGCCGTTCCTGAGTTTCGTGAGTTTCTTTTTTCGCTTTCTAGATCTACTTAAATCACGCTTTGTATTTTGTTATTCGCACGATCTTTACTCTACTTGCGACTGGTGTTCTGTGTTAATTTTTTACATTTCAATCGCTTGGATGTTTTTGGTTCTTACCCTGTTTTAGGATAATGGGTTTTGTTTCCTCCCCCCCTTTTTTTTGGCCCCCCATGGAAATCTGTGAAGATTCAATGATGAATGCGATCATCCTTGCTATGATAATACGACCAAGTTGCTCCATTAATGCTAACCCACTTCATTATTGCGTTGTCCTTTTCCCAATCCTCCGATGTCTTCTATTTCTATCTGTCCATATTTTCTACAAATAGTAGTTGAATAGACCTGTCTAAATGGGCTTGGTTTTCCTTCTGTATAAAGACCTCGCTGTAATCTAAAGAATCAAATCCATGATTTTAGAGGGAGCTATTCCGTGAGATTGAAGGAACTGAGAAGACCCAGAATCCTTGTTGTGTATTAACAGTGAATGAATAAACAAGTTATTAAGTCACTGAAAGGAGTTGATGTTTGGCTATCGTTTTGTAATCTGTCAGAGCATGGAATTTCGTGGAGTAAAAGAATCCTAAAGAAGGAGGATGTATTAAGAACTGAGAATTCCGAATATCGTTCGAAAGAGTTGCAGGGAAGGTACACTGTCTCATATTAAGTCACTGAAAGGACTTGATGGAGAAAATATCTGAGGTTTCAAGCCTCTACTGTGTCATGTTGCAATTTGAATGGCAGTCGTTGAGCCCGTGGAACAACTTAATTGAATACAACTAAGTGTCTTTACAGTTTATTTACCTTGTCCTCTTAGTTTTGGAAATGAAAAGATATGTGATGACTTGTTTCAGCACATTTTTATATGGAACATGAAAATTGAATATATATATATATATATAAAGTATATATATATATAAAGGTTCTTTTATGTATTTGATAAGGATGAAAGAAAAATTAACTTAATCAAACAAAGTAGCTGAACCCAAAGAATTGTATGATGATGAAGAAACAGAATCCACTAGCCCTTGATGTTGCAGGAGTGAAACACAGGGATTTTTGGAACTTTTAATTTCTATATAATACACAGCCTCTAGTTGGAGAGCCTTGGATTGAACTTACAGTAACGATTATCTTAATAAATATCTATTGCGGGTGCTTCTTATAATCAACCATTTTGTGGCTTAGGAGATGTCCTTTCAGCCTGCTCTTTGTAACCTTGGATTCTAACTAGCTATTCGTGTTTCTTATATATGTGTTTATTCTTATAAAAAAAATCTGGGTGTTATGAACTTATGTACTTATCATTGTCTTCTAGATAGTGATAGAACTGTGACAAATTTAGACCTTTGAGAGGCTTGGTTTCTTGCAGGGCACGCCGAACTGAACTTTTATGCCTCAAGCTTTTGATCGTGCTTCAAGATTGAGCAAACGCTTCTCTCATAATCCCCAGATTTTTGGTTTATCCTCTGTTGTAAGTGGTAGCAGCCATCATTTTAGACGTTCTAGTGCTATGAAAGAGATTAAAGTCAAAGGTGGTTCTAATGCAGATCCTCATCTGTTTAACGATGAATCACATGCACAGTCTTGTGCAAATCAGGGATGTTCAGCATCAAATGTGCAGTGCACCCCTGAAATTGAAAAGAAGTATGTGCATCGTGTTTACGATGCCATTGCACCCCATTTTAGTTCCACTCGATTTGCAAAGTGGCCAAAAGTTGCATCATTCTTATCCTCCTTACCTCTAGGGTCTCTCGTCCTGGATGCTGGCTGTGGTAATGGAAAGTACTTGGGCTATAATCCCAACTGCTTTTTTATTGGATGTGATATTAGTGCCCAACTTATCAAAATTTGTAACGAAAGGGGGCATGAAGTTTTGGTGGCGGACGCTGTAAATCTTCCTTACAGAACTGGTTTTGGCGATGCAGCAATCTCTATAGCTGTATTACATCATCTAAGTACTGAGAACAGGAGGAAGAAAGCGATTGAAGAATTAATACGCGTTGTCAAAAAAGGTGGCCTAGTTTTAATAACAGTCTGGGCTGTTGAACAAGAGGACAAATCTTTATTGACGAAATGGATGCCACTTTCAGAAAAGTATGTTGAAGAGTGGGTTGGACCTGGTAGTCCGCGCGTTCGTAGTCCATCGTCATTGGCACTTGAAAGCATTCCTGAAATGAATGAAAATAATTCGGGTATTTGTCTAAGGGATTCCAAGGAAAACGTTACTGGAAGCATACCAGAAAATAAACCTCTACCCTCTCAAACTGAAAATGATTTGGCGAATTGCAACGATGAAAATTTATTTAAGACTCAGCAAGAGTATTTTGTCCCATGGCACTTACCTTATCATCGTGCAGAAGTCAGTGGCACGTCTGCCAGTGCCCTTGCTAGTGGTTTGGCAAAAAAAGATGATAAGAAAGCAGCTGTTGTGTATAACAGATACTATCATGTTTTCAGTGAAGGCGAACTTGAAAGGTATTACTCTCCCTCATCTCTATTGTATGATTTGTTTTTCTTGTCTCCTTTCGGTGATTGGGTATGAGTGAGTCTCTTGAATACATCTCATTTCAATGTGACTGTGCTGGTCTAGATCTAGTGTGTTCAACTGTATTTCTCATTCCCTTCCTCTGATACTCAATGCATTACATAAGCCATATACTTTTTGGTAAAAGATTAGCTGGATGAAAGTTGGAACAAAGGGAATGATGTAGACATGTTTTTTAATGTTAGCAAAAAAAAAAAAAAAAAAATCGTAATGTACTAAGTAACAACGTTGCAAGATGCACCCGTATCAGTACAGTCGATTTTTGCACATGGGAGATACAGATGATGCTGGTCCGTTGATTACTCATTCTCCATTTTAGAAATGTATGGGAAAAAGAACATTATACTTTTCCTAGCTTTGGCTGTACAGATTATTATTTCTGTTCGTTCAAAATCGAATAGAAAATCCTTCCTAGAAAAGACATCTACAAAAAGGGAGGGGAGATAAGAAAACATCCATACGACAAGACAAAAAGAAAGATCACAATAGAACTCCCTAATTGGTAGCCAAAAAAAAAAAAAAAGGGAAAGGAGAATACAGTTTTGAGGCCAAAAGGGAAAAAAAAAGGACAGGACGGGGAATTTGGTAAAGGCGCCTAAAATTAAGCTTAATCTTGTTTAGATGTCTAATTTGGGGTATTAGTGGCATATACTAATTTGCTCCTGGAGCACAGTACCTTGTTACTAACTAGTTCCAGTTCCATTAATATGTGATGAGGTTAGAACTGGTAACCAGGATCATTCGTCATTTTGGTATCATTCCATCTTTTGACTTAAGATGCCATAGCTGTATAATTAAGGAACTCAGTATATTGTATTTGAAGACAAATTTTCATTTCATTCCGTTATCCTCTTGATATACATTTCTACATAATAATTTGTTCTTTTACTGCTTTCTCATCATGGGTGCATTTGCAGGTTGATTTCTGGTATGGACAATGCAGTAGTGGTTGATCGTTTTTACGACAAATCAAACTGGTGTATAGTTCTTGAGAAGACGGTGTGAGTATTTAAACGAGTTCATTGCCTTTATGAAGATATGGTTTGAGCATGTCTGCCTGCTGTGCGAACTCAAGTCAGGCTTTCAAGATAACTCGCCGTGCTAAAGGCTGTTGCTATCTCGGCTTTGTTCGTGTCGTCCAGTCAAGGTTTGGGGATGGAAAATCTGCAGGCTCTCTTGCAGAAAATGCACGCACTGTAAGGATTTACAATTGTATTATGATTAATAATGCGCTGGATTGCCCTTTGGTTCAGTTCTAA

mRNA sequence

TTATAATTTAACAAAATAATTAACTCTGCAAAATCAATGTAGAGTCACTAGGGCTTCCTCTTCACTTGACGACGAAGACGACCAACCACTTGAAGCTGCCATTGCGAGATTGGTGCCTTGATCTCCTCTTCTTCTTCTTCTTCTTTCCTCTCTTTTTCTTTCTTTTTCCAATCACGAAACTACATTCCACAATCTCAACCTTCCATTTGTTTTTCTCTAATCTTTCAGTTTAATCATGCAGCCACGCCGTTCCTGAGTTTCAGCATGGAATTTCGTGGAGTAAAAGAATCCTAAAGAAGGAGGATGTATTAAGAACTGAGAATTCCGAATATCGTTCGAAAGAGTTGCAGGGAAGGGCACGCCGAACTGAACTTTTATGCCTCAAGCTTTTGATCGTGCTTCAAGATTGAGCAAACGCTTCTCTCATAATCCCCAGATTTTTGGTTTATCCTCTGTTGTAAGTGGTAGCAGCCATCATTTTAGACGTTCTAGTGCTATGAAAGAGATTAAAGTCAAAGGTGGTTCTAATGCAGATCCTCATCTGTTTAACGATGAATCACATGCACAGTCTTGTGCAAATCAGGGATGTTCAGCATCAAATGTGCAGTGCACCCCTGAAATTGAAAAGAAGTATGTGCATCGTGTTTACGATGCCATTGCACCCCATTTTAGTTCCACTCGATTTGCAAAGTGGCCAAAAGTTGCATCATTCTTATCCTCCTTACCTCTAGGGTCTCTCGTCCTGGATGCTGGCTGTGGTAATGGAAAGTACTTGGGCTATAATCCCAACTGCTTTTTTATTGGATGTGATATTAGTGCCCAACTTATCAAAATTTGTAACGAAAGGGGGCATGAAGTTTTGGTGGCGGACGCTGTAAATCTTCCTTACAGAACTGGTTTTGGCGATGCAGCAATCTCTATAGCTGTATTACATCATCTAAGTACTGAGAACAGGAGGAAGAAAGCGATTGAAGAATTAATACGCGTTGTCAAAAAAGGTGGCCTAGTTTTAATAACAGTCTGGGCTGTTGAACAAGAGGACAAATCTTTATTGACGAAATGGATGCCACTTTCAGAAAAGTATGTTGAAGAGTGGGTTGGACCTGGTAGTCCGCGCGTTCGTAGTCCATCGTCATTGGCACTTGAAAGCATTCCTGAAATGAATGAAAATAATTCGGGTATTTGTCTAAGGGATTCCAAGGAAAACGTTACTGGAAGCATACCAGAAAATAAACCTCTACCCTCTCAAACTGAAAATGATTTGGCGAATTGCAACGATGAAAATTTATTTAAGACTCAGCAAGAGTATTTTGTCCCATGGCACTTACCTTATCATCGTGCAGAAGTCAGTGGCACGTCTGCCAGTGCCCTTGCTAGTGGTTTGGCAAAAAAAGATGATAAGAAAGCAGCTGTTGTGTATAACAGATACTATCATGTTTTCAGTGAAGGCGAACTTGAAAGGTTGATTTCTGGTATGGACAATGCAGTAGTGGTTGATCGTTTTTACGACAAATCAAACTGGTGTATAGTTCTTGAGAAGACGGCTTTCAAGATAACTCGCCGTGCTAAAGGCTGTTGCTATCTCGGCTTTGTTCGTGTCGTCCAGTCAAGGTTTGGGGATGGAAAATCTGCAGGCTCTCTTGCAGAAAATGCACGCACTGTAAGGATTTACAATTGTATTATGATTAATAATGCGCTGGATTGCCCTTTGGTTCAGTTCTAA

Coding sequence (CDS)

ATGCCTCAAGCTTTTGATCGTGCTTCAAGATTGAGCAAACGCTTCTCTCATAATCCCCAGATTTTTGGTTTATCCTCTGTTGTAAGTGGTAGCAGCCATCATTTTAGACGTTCTAGTGCTATGAAAGAGATTAAAGTCAAAGGTGGTTCTAATGCAGATCCTCATCTGTTTAACGATGAATCACATGCACAGTCTTGTGCAAATCAGGGATGTTCAGCATCAAATGTGCAGTGCACCCCTGAAATTGAAAAGAAGTATGTGCATCGTGTTTACGATGCCATTGCACCCCATTTTAGTTCCACTCGATTTGCAAAGTGGCCAAAAGTTGCATCATTCTTATCCTCCTTACCTCTAGGGTCTCTCGTCCTGGATGCTGGCTGTGGTAATGGAAAGTACTTGGGCTATAATCCCAACTGCTTTTTTATTGGATGTGATATTAGTGCCCAACTTATCAAAATTTGTAACGAAAGGGGGCATGAAGTTTTGGTGGCGGACGCTGTAAATCTTCCTTACAGAACTGGTTTTGGCGATGCAGCAATCTCTATAGCTGTATTACATCATCTAAGTACTGAGAACAGGAGGAAGAAAGCGATTGAAGAATTAATACGCGTTGTCAAAAAAGGTGGCCTAGTTTTAATAACAGTCTGGGCTGTTGAACAAGAGGACAAATCTTTATTGACGAAATGGATGCCACTTTCAGAAAAGTATGTTGAAGAGTGGGTTGGACCTGGTAGTCCGCGCGTTCGTAGTCCATCGTCATTGGCACTTGAAAGCATTCCTGAAATGAATGAAAATAATTCGGGTATTTGTCTAAGGGATTCCAAGGAAAACGTTACTGGAAGCATACCAGAAAATAAACCTCTACCCTCTCAAACTGAAAATGATTTGGCGAATTGCAACGATGAAAATTTATTTAAGACTCAGCAAGAGTATTTTGTCCCATGGCACTTACCTTATCATCGTGCAGAAGTCAGTGGCACGTCTGCCAGTGCCCTTGCTAGTGGTTTGGCAAAAAAAGATGATAAGAAAGCAGCTGTTGTGTATAACAGATACTATCATGTTTTCAGTGAAGGCGAACTTGAAAGGTTGATTTCTGGTATGGACAATGCAGTAGTGGTTGATCGTTTTTACGACAAATCAAACTGGTGTATAGTTCTTGAGAAGACGGCTTTCAAGATAACTCGCCGTGCTAAAGGCTGTTGCTATCTCGGCTTTGTTCGTGTCGTCCAGTCAAGGTTTGGGGATGGAAAATCTGCAGGCTCTCTTGCAGAAAATGCACGCACTGTAAGGATTTACAATTGTATTATGATTAATAATGCGCTGGATTGCCCTTTGGTTCAGTTCTAA

Protein sequence

MPQAFDRASRLSKRFSHNPQIFGLSSVVSGSSHHFRRSSAMKEIKVKGGSNADPHLFNDESHAQSCANQGCSASNVQCTPEIEKKYVHRVYDAIAPHFSSTRFAKWPKVASFLSSLPLGSLVLDAGCGNGKYLGYNPNCFFIGCDISAQLIKICNERGHEVLVADAVNLPYRTGFGDAAISIAVLHHLSTENRRKKAIEELIRVVKKGGLVLITVWAVEQEDKSLLTKWMPLSEKYVEEWVGPGSPRVRSPSSLALESIPEMNENNSGICLRDSKENVTGSIPENKPLPSQTENDLANCNDENLFKTQQEYFVPWHLPYHRAEVSGTSASALASGLAKKDDKKAAVVYNRYYHVFSEGELERLISGMDNAVVVDRFYDKSNWCIVLEKTAFKITRRAKGCCYLGFVRVVQSRFGDGKSAGSLAENARTVRIYNCIMINNALDCPLVQF
Homology
[Close]
BLAST of Lsi03G004450 vs. Swiss-Prot
Match: ALKB8_MOUSE (Alkylated DNA repair protein alkB homolog 8 OS=Mus musculus GN=Alkbh8 PE=1 SV=1)

HSP 1 Score: 217.6 bits (553), Expect = 2.7e-55
Identity = 121/313 (38.66%), Postives = 176/313 (56.23%), Query Frame = 1 

Query: 81  EIEKKYVHRVYDAIAPHFSSTRFAKWPKVASFLSSLPLGSLVLDAGCGNGKYLGYNPNCF 140
           E+E+K+VH+VY+ IA HFSSTR + WP++  FL +LP GS+V D GCGNGKYLG N + +
Sbjct: 369 ELEQKHVHQVYNEIASHFSSTRHSPWPRIVEFLKALPSGSIVADIGCGNGKYLGINKDLY 428

Query: 141 FIGCDISAQLIKICNERGHEVLVADAVNLPYRTGFGDAAISIAVLHHLSTENRRKKAIEE 200
            IGCD S  L+ IC ER  + LV DA+ +P R+G  DA ISIAV+HH +T  RR +A++E
Sbjct: 429 MIGCDRSQNLVDICRERQFQALVCDALAVPVRSGSCDACISIAVIHHFATAERRVEALQE 488

Query: 201 LIRVVKKGGLVLITVWAVEQEDKSLLTKWMPLSEKYVEEWVGPGSPRVRSPSSLALESIP 260
           L R+++ GG  LI VWA+EQE K+  +K+  L  K + +          S     +   P
Sbjct: 489 LARLLRPGGQALIYVWAMEQEYKNQKSKY--LRGKRISQGDKDELNSATSTEEFLVNQTP 548

Query: 261 EMNENNSGICLRDS-----KENVTGSIPENKPLPSQTENDLANCNDENLFKTQQEYFVPW 320
           E    +  + +  S     +E  +  +P N  LP        +          Q+  VPW
Sbjct: 549 EGVNEDPALSVNSSSITKEEEYKSRKVP-NSELPIHINRTCFH---------SQDVLVPW 608

Query: 321 HLPYHRAEVSGTSASALASGLAKKDDKKAAVVYNRYYHVFSEGELERLISGMDNAVVVDR 380
           HL  +     G   +   SG+A   D   + V++RYYHVF +GELE     + +  ++  
Sbjct: 609 HLKRN----PGKDKAIEPSGVAGCPD--PSPVFHRYYHVFCDGELEASCQAVGDVSILQS 663

Query: 381 FYDKSNWCIVLEK 389
           +YD+ NWC+VL+K
Sbjct: 669 YYDQGNWCVVLQK 663

[Close]
BLAST of Lsi03G004450 vs. Swiss-Prot
Match: ALKB8_BOVIN (Alkylated DNA repair protein alkB homolog 8 OS=Bos taurus GN=ALKBH8 PE=2 SV=1)

HSP 1 Score: 214.5 bits (545), Expect = 2.3e-54
Identity = 119/317 (37.54%), Postives = 167/317 (52.68%), Query Frame = 1 

Query: 81  EIEKKYVHRVYDAIAPHFSSTRFAKWPKVASFLSSLPLGSLVLDAGCGNGKYLGYNPNCF 140
           ++E+ YVHRVY+ IA HFSSTR   WP +  FL +LP GSLV D GCGNGKYLG N   +
Sbjct: 369 QLEQDYVHRVYEEIARHFSSTRHTPWPHIVEFLKALPSGSLVADIGCGNGKYLGINNELY 428

Query: 141 FIGCDISAQLIKICNERGHEVLVADAVNLPYRTGFGDAAISIAVLHHLSTENRRKKAIEE 200
            IGCD S  L+ IC ER ++  V DA+ +P R+G  DA ISIAV+HH +T  RR  A++E
Sbjct: 429 MIGCDHSQNLVDICRERQYQAFVCDALAVPIRSGSCDACISIAVIHHFATAERRVAALQE 488

Query: 201 LIRVVKKGGLVLITVWAVEQEDKSLLTKWMPLSEKYVEEWVGPGSPRVRSPSSLALESIP 260
           L+R+++ GG  LI VWA+EQE     +K+  L E    + +        S   L ++ +P
Sbjct: 489 LVRLLRPGGKALIYVWAMEQEYNKKKSKY--LRENRTSQGMKEEISNDASVQELLVKQLP 548

Query: 261 EMNENNSGICLRDSKENVTGSIPE----NKPLPSQTENDLANCNDENLFKTQQEYFVPWH 320
            +   +S   +    +   G        N  LP  T     +          Q+  VPWH
Sbjct: 549 NVGNQDSAHSVSSINDFQDGGCNSKNVANSKLPIHTNRTSFH---------SQDLLVPWH 608

Query: 321 LPYH-----RAEVSGTSASALASGLAKKDDKKAAVVYNRYYHVFSEGELERLISGMDNAV 380
              +     R E  G   S           +    V++RYYHVF EGELE     ++N  
Sbjct: 609 FKGNPGKDKRVEPFGPLGS-----------RDPGPVFHRYYHVFCEGELEAACRTLNNIS 663

Query: 381 VVDRFYDKSNWCIVLEK 389
           ++  +YD+ NWC++L+K
Sbjct: 669 ILQSYYDQGNWCVILQK 663

[Close]
BLAST of Lsi03G004450 vs. Swiss-Prot
Match: ALKB8_MACFA (Alkylated DNA repair protein alkB homolog 8 OS=Macaca fascicularis GN=ALKBH8 PE=2 SV=1)

HSP 1 Score: 210.3 bits (534), Expect = 4.3e-53
Identity = 114/319 (35.74%), Postives = 172/319 (53.92%), Query Frame = 1 

Query: 82  IEKKYVHRVYDAIAPHFSSTRFAKWPKVASFLSSLPLGSLVLDAGCGNGKYLGYNPNCFF 141
           +E++YVH+VY+ IA HFSSTR   WP +  FL +LP GS+V D GCGNGKYLG N   + 
Sbjct: 370 LEQEYVHQVYEEIAGHFSSTRHTPWPHIVEFLKALPSGSIVADIGCGNGKYLGVNKELYM 429

Query: 142 IGCDISAQLIKICNERGHEVLVADAVNLPYRTGFGDAAISIAVLHHLSTENRRKKAIEEL 201
           +GCD S  L+ IC ER  +  V DA+ +P R+G  DA ISIAV+HH +T  RR  A++E+
Sbjct: 430 VGCDRSQNLVDICRERQFQAFVCDALAVPVRSGSCDACISIAVIHHFATAERRVAALQEI 489

Query: 202 IRVVKKGGLVLITVWAVEQEDKSLLTKWMP------------LSEKYVEEWVGPGSPRVR 261
           +R+++ GG  LI VWA+EQE     +K++              S+  V+  +    P + 
Sbjct: 490 VRLLRPGGKALIYVWAMEQEYNKQKSKYLKGNRNSQGKKEEMNSDTSVQRSLVEQMPDMG 549

Query: 262 SPSSLALESIPEMNENNSGICLRDSKENVTGSIPENKPLPSQTENDLANCNDENLFKTQQ 321
           S  S +  S+P +N++  G C  +S++     +P              + N  + +   Q
Sbjct: 550 SRDSAS--SVPRINDSQEGGC--NSRQVSNSKLP-------------IHVNRTSFY--SQ 609

Query: 322 EYFVPWHLPYHRAEVSGTSASALASGLAKKDDKKAAVVYNRYYHVFSEGELERLISGMDN 381
           +  VPWHL                        +  + V++RYYHVF EGELE L   + +
Sbjct: 610 DMLVPWHLK------GNPDKGKPVEPFGPIGSQDPSPVFHRYYHVFREGELEALCRTVSD 663

Query: 382 AVVVDRFYDKSNWCIVLEK 389
             ++  +YD+ NWC++L+K
Sbjct: 670 VRILQSYYDQGNWCVILQK 663

[Close]
BLAST of Lsi03G004450 vs. Swiss-Prot
Match: ALKB8_HUMAN (Alkylated DNA repair protein alkB homolog 8 OS=Homo sapiens GN=ALKBH8 PE=1 SV=2)

HSP 1 Score: 206.8 bits (525), Expect = 4.8e-52
Identity = 116/317 (36.59%), Postives = 170/317 (53.63%), Query Frame = 1 

Query: 82  IEKKYVHRVYDAIAPHFSSTRFAKWPKVASFLSSLPLGSLVLDAGCGNGKYLGYNPNCFF 141
           +E++YVH+VY+ IA HFSSTR   WP +  FL +LP GS+V D GCGNGKYLG N   + 
Sbjct: 370 LEQEYVHQVYEEIAGHFSSTRHTPWPHIVEFLKALPSGSIVADIGCGNGKYLGINKELYM 429

Query: 142 IGCDISAQLIKICNERGHEVLVADAVNLPYRTGFGDAAISIAVLHHLSTENRRKKAIEEL 201
           IGCD S  L+ IC ER  +  V DA+ +P R+G  DA ISIAV+HH +T  RR  A++E+
Sbjct: 430 IGCDRSQNLVDICRERQFQAFVCDALAVPVRSGSCDACISIAVIHHFATAERRVAALQEI 489

Query: 202 IRVVKKGGLVLITVWAVEQE---DKSLLTKWMPLSEKYVEEWVGPGS------PRVRSPS 261
           +R+++ GG  LI VWA+EQE    KS   +    S+   EE     S       ++R   
Sbjct: 490 VRLLRPGGKALIYVWAMEQEYNKQKSKYLRGNRNSQGKKEEMNSDTSVQRSLVEQMRDMG 549

Query: 262 SL-ALESIPEMNENNSGICLRDSKENVTGSIPENKPLPSQTENDLANCNDENLFKTQQEY 321
           S  +  S+P +N++  G C  +S++     +P              + N  + +   Q+ 
Sbjct: 550 SRDSASSVPRINDSQEGGC--NSRQVSNSKLP-------------VHVNRTSFY--SQDV 609

Query: 322 FVPWHLPYHRAEVSGTSASALASGLAKKDDKKAAVVYNRYYHVFSEGELERLISGMDNAV 381
            VPWHL                        +  + V++RYYHVF EGELE     + +  
Sbjct: 610 LVPWHLK------GNPDKGKPVEPFGPIGSQDPSPVFHRYYHVFREGELEGACRTVSDVR 663

Query: 382 VVDRFYDKSNWCIVLEK 389
           ++  +YD+ NWC++L+K
Sbjct: 670 ILQSYYDQGNWCVILQK 663

[Close]
BLAST of Lsi03G004450 vs. Swiss-Prot
Match: ALKB8_XENTR (Alkylated DNA repair protein alkB homolog 8 OS=Xenopus tropicalis GN=alkbh8 PE=2 SV=2)

HSP 1 Score: 194.9 bits (494), Expect = 1.9e-48
Identity = 116/314 (36.94%), Postives = 163/314 (51.91%), Query Frame = 1 

Query: 82  IEKKYVHRVYDAIAPHFSSTRFAKWPKVASFLSSLPLGSLVLDAGCGNGKYLGYNPNCFF 141
           +E++YVH+VYD IA HFSSTR   WPK+  FL+SLP GSLV D GCGNGKYLG N +   
Sbjct: 369 LEREYVHKVYDDIAGHFSSTRHTPWPKIGDFLASLPKGSLVADVGCGNGKYLGVNKDLCM 428

Query: 142 IGCDISAQLIKICNERGHEVLVADAVNLPYRTGFGDAAISIAVLHHLSTENRRKKAIEEL 201
           IGCD S  L+ IC ER  E  V DA+++P+R G  DA ISIAV+HH +TE RR  A++E 
Sbjct: 429 IGCDRSKNLVDICRERTFEAFVCDALSVPFRAGAFDACISIAVIHHFATEERRIAALQE- 488

Query: 202 IRVVKKGGLVLITVWAVEQEDKSLLTKWMPLSE------KYVEEWVGPGSPRVRSPSSLA 261
                        +  + +  K+L+  W    E      KY++E     S   + PSS A
Sbjct: 489 ------------LIRLLRKGGKALIYVWALEQEYKKNKSKYLKE-----SKTSQGPSSDA 548

Query: 262 LESIPEMNENNSGICLRDSKENVTGSIPENKPLPSQTENDLANCNDENLFKTQQEYFVPW 321
           L +                      ++    PLP  T     +          Q+  VPW
Sbjct: 549 LGT----------------------NLAVKAPLPIHTNRTSFH---------SQDLLVPW 608

Query: 322 HL-PYHRAEVSGTSASALASGLAKKDDKKAAVVYNRYYHVFSEGELERLISGMDNAVVVD 381
           HL P ++++V+  +          + +     VY+R+YHVF EGELE + + + N  V  
Sbjct: 609 HLKPTNKSKVTPENKEQ------NEKEHGPDSVYHRFYHVFCEGELEAMCNRLSNVAVQH 627

Query: 382 RFYDKSNWCIVLEK 389
            ++D+ NWC++LEK
Sbjct: 669 SYHDQGNWCVILEK 627

[Close]
BLAST of Lsi03G004450 vs. TrEMBL
Match: A0A0A0LMT8_CUCSA (Uncharacterized protein OS=Cucumis sativus GN=Csa_2G403730 PE=4 SV=1)

HSP 1 Score: 729.9 bits (1883), Expect = 1.8e-207
Identity = 360/389 (92.54%), Postives = 373/389 (95.89%), Query Frame = 1 

Query: 1   MPQAFDRASRLSKRFSHNPQIFGLSSVVSGSSHHFRRSSAMKEIKVKGGSNADPHLFNDE 60
           M Q+FDR+SR+SK F H+ +IFGLSSVV GS HHFR +SAMKEIK+KGGSNADPH  NDE
Sbjct: 1   MLQSFDRSSRVSKYFCHSCKIFGLSSVVIGSGHHFRLNSAMKEIKIKGGSNADPHPLNDE 60

Query: 61  SHAQ-SCANQGCSASNVQCTPEIEKKYVHRVYDAIAPHFSSTRFAKWPKVASFLSSLPLG 120
           +HAQ SCA QGC+ASNVQCTPEIEKKYVHRVYDAIAPHFSSTRFAKWPKVASFLSSLPLG
Sbjct: 61  THAQLSCAIQGCTASNVQCTPEIEKKYVHRVYDAIAPHFSSTRFAKWPKVASFLSSLPLG 120

Query: 121 SLVLDAGCGNGKYLGYNPNCFFIGCDISAQLIKICNERGHEVLVADAVNLPYRTGFGDAA 180
           SLVLDAGCGNGKYLGYN NCFFIGCDISAQLIKICNERGHEVLVADAVNLPYRTGFGDAA
Sbjct: 121 SLVLDAGCGNGKYLGYNSNCFFIGCDISAQLIKICNERGHEVLVADAVNLPYRTGFGDAA 180

Query: 181 ISIAVLHHLSTENRRKKAIEELIRVVKKGGLVLITVWAVEQEDKSLLTKWMPLSEKYVEE 240
           ISIAVLHHLSTENRRKKAIEELIRVVKKGGLVLITVWAVEQEDKSLLTKWMPLSEKYVEE
Sbjct: 181 ISIAVLHHLSTENRRKKAIEELIRVVKKGGLVLITVWAVEQEDKSLLTKWMPLSEKYVEE 240

Query: 241 WVGPGSPRVRSPSSLALESIPEMNENNSGICLRDSKENVTGSIPENKPLPSQTENDLANC 300
           WVGPGSPRVRSPSS+ALESIPEMNENNSGICL+DSKEN+TGSIPENKP PSQ+ENDLANC
Sbjct: 241 WVGPGSPRVRSPSSMALESIPEMNENNSGICLKDSKENLTGSIPENKPPPSQSENDLANC 300

Query: 301 NDENLFKTQQEYFVPWHLPYHRAEVSGTSASALASGLAKKDDKKAAVVYNRYYHVFSEGE 360
           N ENL KTQQEYFVPWHLPYHRAEVSGTSASALASGLAKKDDKKAAVVYNRYYHVFSEGE
Sbjct: 301 NHENLLKTQQEYFVPWHLPYHRAEVSGTSASALASGLAKKDDKKAAVVYNRYYHVFSEGE 360

Query: 361 LERLISGMDNAVVVDRFYDKSNWCIVLEK 389
           LE LISGMDNAVVVDRFYDKSNWCIVLEK
Sbjct: 361 LEGLISGMDNAVVVDRFYDKSNWCIVLEK 389

[Close]
BLAST of Lsi03G004450 vs. TrEMBL
Match: A0A067KLS3_JATCU (Uncharacterized protein OS=Jatropha curcas GN=JCGZ_12011 PE=4 SV=1)

HSP 1 Score: 535.4 bits (1378), Expect = 6.6e-149
Identity = 264/354 (74.58%), Postives = 295/354 (83.33%), Query Frame = 1 

Query: 41  MKEIKVKGGSNADPHLFNDESHAQS----CANQGCSASNVQCTPEIEKKYVHRVYDAIAP 100
           M++IKVKG S    H F+ ES  Q     C  Q C + +VQ TPEIE+KYVHRVYDAIAP
Sbjct: 1   MRKIKVKGSSGLFSHAFDGESQIQQSVSECEEQKCLSCSVQSTPEIERKYVHRVYDAIAP 60

Query: 101 HFSSTRFAKWPKVASFLSSLPLGSLVLDAGCGNGKYLGYNPNCFFIGCDISAQLIKICNE 160
           HFSSTRFAKWPKVA+FL+SLP GSL+LDAGCGNGKYLG NP+CFFIGCDISA LIKIC +
Sbjct: 61  HFSSTRFAKWPKVATFLNSLPSGSLILDAGCGNGKYLGLNPDCFFIGCDISAPLIKICAD 120

Query: 161 RGHEVLVADAVNLPYRTGFGDAAISIAVLHHLSTENRRKKAIEELIRVVKKGGLVLITVW 220
           RGHEVLV DAVNLPYRTGFGDAAISIAVLHHLSTENRRKKAIEEL+RVVKKGGLVLITVW
Sbjct: 121 RGHEVLVGDAVNLPYRTGFGDAAISIAVLHHLSTENRRKKAIEELVRVVKKGGLVLITVW 180

Query: 221 AVEQEDKSLLTKWMPLSEKYVEEWVGPGSPRVRSPSSLALESIPEMNENNSGICLRDSKE 280
           AVEQED SL+TKW PL+++Y+EEW+GPGSPRVRSPSS+ LESIPE+ ENNS   ++DSKE
Sbjct: 181 AVEQEDGSLVTKWTPLTQRYLEEWIGPGSPRVRSPSSITLESIPEIEENNSKDHVKDSKE 240

Query: 281 NVTGSIPENKPLPSQTENDLANCNDENLFKTQQEYFVPWHLPYHRAEVSGTSASALASGL 340
                + +   L SQTE D     DE     QQEYFVPWHLPYHRAEVSG SA AL SGL
Sbjct: 241 KADKELKKMTHLTSQTEMDGIG-KDEKNANNQQEYFVPWHLPYHRAEVSGASACALESGL 300

Query: 341 AKKDDKKAAVVYNRYYHVFSEGELERLISGMDNAVVVDRFYDKSNWCIVLEKTA 391
           AKKDDKK AV+YNRYYHVFSEGEL+RL+ GM+N VVVDRF+DKSNWCI+LEKT+
Sbjct: 301 AKKDDKKGAVIYNRYYHVFSEGELDRLVIGMNNVVVVDRFFDKSNWCIILEKTS 353

[Close]
BLAST of Lsi03G004450 vs. TrEMBL
Match: B9RJD9_RICCO (S-adenosylmethionine-dependent methyltransferase, putative OS=Ricinus communis GN=RCOM_1033330 PE=4 SV=1)

HSP 1 Score: 534.3 bits (1375), Expect = 1.5e-148
Identity = 276/388 (71.13%), Postives = 308/388 (79.38%), Query Frame = 1 

Query: 7   RASRLSKRFSHNPQIFGLSSVVSGSSHHFRRSSAMKEIKVKGGSNADPHLFNDESHAQSC 66
           RASR S+ +    Q F    +V G    F    AM+EIKVKG S       + ES  Q  
Sbjct: 7   RASRFSETYR---QAFARPIIVIGRVCLFGIFRAMREIKVKGPSGLSNVASDGESQIQLS 66

Query: 67  AN----QGCSASNVQCTPEIEKKYVHRVYDAIAPHFSSTRFAKWPKVASFLSSLPLGSLV 126
            +    Q CS+S+VQ TPEIEKKYVH VYDAIAPHFSSTRFAKWPKVA+FL+SLP GSL+
Sbjct: 67  LSADEKQVCSSSSVQSTPEIEKKYVHHVYDAIAPHFSSTRFAKWPKVATFLNSLPSGSLI 126

Query: 127 LDAGCGNGKYLGYNPNCFFIGCDISAQLIKICNERGHEVLVADAVNLPYRTGFGDAAISI 186
           LDAGCGNGKYLG NP+C+FIGCDISA LIKIC +RGHEVLV DAVNLPYRTGF DAAISI
Sbjct: 127 LDAGCGNGKYLGLNPDCYFIGCDISAPLIKICADRGHEVLVGDAVNLPYRTGFSDAAISI 186

Query: 187 AVLHHLSTENRRKKAIEELIRVVKKGGLVLITVWAVEQEDKSLLTKWMPLSEKYVEEWVG 246
           AVLHHLSTENRRKKAIEEL+RVVKKGGLVLITVWAVEQED SL+ KW PLS+KYVEEW+G
Sbjct: 187 AVLHHLSTENRRKKAIEELVRVVKKGGLVLITVWAVEQEDGSLVAKWTPLSQKYVEEWIG 246

Query: 247 PGSPRVRSPSSLALESIPEMNENNSGICLRDSKENVTGSIPENKPLPSQTENDLANCNDE 306
           PGSPRVRSPSS  LESIPE  ENNS   ++DS++N      +     SQTE++ +   DE
Sbjct: 247 PGSPRVRSPSSFTLESIPETEENNSKELIQDSEKNADEEFMKTMDSISQTEHN-SVFKDE 306

Query: 307 NLFKTQQEYFVPWHLPYHRAEVSGTSASALASGLAKKDDKKAAVVYNRYYHVFSEGELER 366
              K QQEYFVPWHLPYHRAEVSG SA ALASGLAKKDDKK AVVYNRYYHVFSEGELER
Sbjct: 307 KNAKNQQEYFVPWHLPYHRAEVSGASACALASGLAKKDDKKGAVVYNRYYHVFSEGELER 366

Query: 367 LISGMDNAVVVDRFYDKSNWCIVLEKTA 391
           L++GM NAVVVDRF+DKSNWC++LEKT+
Sbjct: 367 LVTGMKNAVVVDRFFDKSNWCVILEKTS 390

[Close]
BLAST of Lsi03G004450 vs. TrEMBL
Match: M5XBE8_PRUPE (Uncharacterized protein OS=Prunus persica GN=PRUPE_ppa007908mg PE=4 SV=1)

HSP 1 Score: 520.8 bits (1340), Expect = 1.7e-144
Identity = 264/355 (74.37%), Postives = 291/355 (81.97%), Query Frame = 1 

Query: 41  MKEIKVKG-------GSNADPHLFNDESHAQSCANQGCSASNVQCTPEIEKKYVHRVYDA 100
           M+E+KVKG        SN + H+   E  + S  N   S+ NVQ TPEIEKKYVH VYDA
Sbjct: 1   MREVKVKGVSSSCTLDSNGESHILQSELASDSQRN---SSLNVQFTPEIEKKYVHHVYDA 60

Query: 101 IAPHFSSTRFAKWPKVASFLSSLPLGSLVLDAGCGNGKYLGYNPNCFFIGCDISAQLIKI 160
           IAPHFSSTRFAKWPKVA+FLSSLP GSLVLDAGCGNGKYLG+NP+CFFIGCDISA LIKI
Sbjct: 61  IAPHFSSTRFAKWPKVANFLSSLPSGSLVLDAGCGNGKYLGFNPDCFFIGCDISAPLIKI 120

Query: 161 CNERGHEVLVADAVNLPYRTGFGDAAISIAVLHHLSTENRRKKAIEELIRVVKKGGLVLI 220
           C +RGHEVLVADAV+LPYRTGFGDAAISIAVLHHLST+ RRKKAIEELIRVVKKGGLVLI
Sbjct: 121 CADRGHEVLVADAVDLPYRTGFGDAAISIAVLHHLSTQGRRKKAIEELIRVVKKGGLVLI 180

Query: 221 TVWAVEQEDKSLLTKWMPLSEKYVEEWVGPGSPRVRSPSSLALESIPEMNENNSGICLRD 280
           TVWAVEQEDKSLL KW PL++KY EEW+GPGSPRVRSPSS  LESIPE  EN  G   +D
Sbjct: 181 TVWAVEQEDKSLLAKWTPLTQKYGEEWIGPGSPRVRSPSSCTLESIPETEENGFGERKQD 240

Query: 281 SKENVTGSIPENKPLPSQTENDLANCNDENLFKTQQEYFVPWHLPYHRAEVSGTSASALA 340
           S E+   S+ E   + SQTE+      D      QQEYFVPWHLPYHRAEVSG SA ALA
Sbjct: 241 SGESSMQSLQE---ITSQTESYSGASTDVKNANNQQEYFVPWHLPYHRAEVSGASACALA 300

Query: 341 SGLAKKDDKKAAVVYNRYYHVFSEGELERLISGMDNAVVVDRFYDKSNWCIVLEK 389
           +GLAKKDDKK AVVYNRYYHVFSEGELERL++G+DNAV+VDRF+DKSNWC+VLEK
Sbjct: 301 NGLAKKDDKKGAVVYNRYYHVFSEGELERLVAGIDNAVIVDRFFDKSNWCVVLEK 349

[Close]
BLAST of Lsi03G004450 vs. TrEMBL
Match: V4SHY6_9ROSI (Uncharacterized protein OS=Citrus clementina GN=CICLE_v10001407mg PE=4 SV=1)

HSP 1 Score: 518.5 bits (1334), Expect = 8.3e-144
Identity = 257/356 (72.19%), Postives = 296/356 (83.15%), Query Frame = 1 

Query: 38  SSAMKEIKVKGGSNADPHLFNDESHAQSCA---NQGCSASNVQCTPEIEKKYVHRVYDAI 97
           S  M+E+KVKG S+      + ES   S +   +Q CS+S+++ TPE+EKKYVHRVYDAI
Sbjct: 39  SGTMREVKVKGASDFCTLGADGESSIHSVSTGEDQRCSSSSIKSTPELEKKYVHRVYDAI 98

Query: 98  APHFSSTRFAKWPKVASFLSSLPLGSLVLDAGCGNGKYLGYNPNCFFIGCDISAQLIKIC 157
           APHFSSTRFAKWPKVA+FL+SLP GSLVLDAGCGNGKYLG NP+CFFIGCDIS  LIKIC
Sbjct: 99  APHFSSTRFAKWPKVATFLNSLPSGSLVLDAGCGNGKYLGLNPDCFFIGCDISPSLIKIC 158

Query: 158 NERGHEVLVADAVNLPYRTGFGDAAISIAVLHHLSTENRRKKAIEELIRVVKKGGLVLIT 217
            +RGHEVLVADAVNLPYR+ FGDAAISIAVLHHLSTE+RRKKAIEEL+RVVKKG LVLIT
Sbjct: 159 ADRGHEVLVADAVNLPYRSDFGDAAISIAVLHHLSTESRRKKAIEELVRVVKKGSLVLIT 218

Query: 218 VWAVEQEDKSLLTKWMPLSEKYVEEWVGPGSPRVRSPSSLALESIPEMNENNSGICLRDS 277
           VWAVEQEDKSL+TKW PL++KYVEEW+GPGSPRVRSPS+  LESIPE  +N S    +D 
Sbjct: 219 VWAVEQEDKSLVTKWTPLTQKYVEEWIGPGSPRVRSPSARTLESIPETEDNGSEEQGKDP 278

Query: 278 KENVTGSIPENKPLPSQTENDLANCNDENLFKTQQEYFVPWHLPYHRAEVSGTSASALAS 337
           K++V   + E+  L SQ+E+D     D  +   QQEYFVPWHLPYHRAEVSG SA ALA+
Sbjct: 279 KQSVPDKLWESISLTSQSEDDSVISQDVKIITNQQEYFVPWHLPYHRAEVSGASACALAN 338

Query: 338 GLAKKDDKKAAVVYNRYYHVFSEGELERLISGMDNAVVVDRFYDKSNWCIVLEKTA 391
           GLAKKDDKK AVVYNRYYHVF +GELERL S +DNAVVVDRF+DKSNWCIVL++T+
Sbjct: 339 GLAKKDDKKGAVVYNRYYHVFCDGELERLASDIDNAVVVDRFFDKSNWCIVLQRTS 394

[Close]
BLAST of Lsi03G004450 vs. TAIR10
Match: AT1G36310.1 (AT1G36310.1 S-adenosyl-L-methionine-dependent methyltransferases superfamily protein)

HSP 1 Score: 474.2 bits (1219), Expect = 9.1e-134
Identity = 252/375 (67.20%), Postives = 284/375 (75.73%), Query Frame = 1 

Query: 38  SSAMKEIKVKGGSNADPHLFNDESHAQSC-ANQGCSASNVQCTPEIEKKYVHRVYDAIAP 97
           +S M++IKVK  S       ++E  + S   +   S S+V+ TPEIEKKYVHRVYDAIAP
Sbjct: 26  TSIMRDIKVKSDSKEFLTSSDEEEESVSIRVSSSSSLSSVKSTPEIEKKYVHRVYDAIAP 85

Query: 98  HFSSTRFAKWPKVASFLSSLPLGSLVLDAGCGNGKYLGYNPNCFFIGCDISAQLIKICNE 157
           HFSSTRFAKWPKVA+FL SLP GS++LDAGCGNGKYLG NP+CFFIGCDIS  LIKIC++
Sbjct: 86  HFSSTRFAKWPKVAAFLESLPSGSVILDAGCGNGKYLGLNPSCFFIGCDISHPLIKICSD 145

Query: 158 RGHEVLVADAVNLPYRTGFGDAAISIAVLHHLSTENRRKKAIEELIRVVKKGGLVLITVW 217
           +G EVLVADAVNLPYR  FGDAAISIAVLHHLSTENRRKKAIEEL+RVVK GG VLITVW
Sbjct: 146 KGQEVLVADAVNLPYREEFGDAAISIAVLHHLSTENRRKKAIEELVRVVKPGGFVLITVW 205

Query: 218 AVEQEDKSLLTKWMPLSEKYVEEWVGPG----SPRVRSPSSLALESIPEMN--------E 277
           A EQED SLLTKW PLS KYVEEWVGPG    SPRVR+    +LESIPE          E
Sbjct: 206 AAEQEDTSLLTKWTPLSAKYVEEWVGPGSPMNSPRVRNNPFFSLESIPETEVSTKEQKVE 265

Query: 278 NNSGICLRDSKENVTGS--------IPENK-PLPSQTENDLANCNDENLFKTQQEYFVPW 337
           N+  I L    E+   +        IPE K  +  Q +      + E L K+QQEYFVPW
Sbjct: 266 NSQFIGLESIPESEESTREQKGESIIPETKASIVEQKDEKSVEESLEALKKSQQEYFVPW 325

Query: 338 HLPYHRAEVSGTSASALASGLAKKDDKKAAVVYNRYYHVFSEGELERLISGMDNAVVVDR 391
           HLPYHRAEVSG SASALASGLAKKDD+K AVVYNRYYHVFSEGELERL SG+ NA++VDR
Sbjct: 326 HLPYHRAEVSGASASALASGLAKKDDRKGAVVYNRYYHVFSEGELERLASGVGNAMIVDR 385

[Close]
BLAST of Lsi03G004450 vs. NCBI nr
Match: gi|449441742|ref|XP_004138641.1| (PREDICTED: alkylated DNA repair protein alkB homolog 8 [Cucumis sativus])

HSP 1 Score: 729.9 bits (1883), Expect = 2.6e-207
Identity = 360/389 (92.54%), Postives = 373/389 (95.89%), Query Frame = 1 

Query: 1   MPQAFDRASRLSKRFSHNPQIFGLSSVVSGSSHHFRRSSAMKEIKVKGGSNADPHLFNDE 60
           M Q+FDR+SR+SK F H+ +IFGLSSVV GS HHFR +SAMKEIK+KGGSNADPH  NDE
Sbjct: 1   MLQSFDRSSRVSKYFCHSCKIFGLSSVVIGSGHHFRLNSAMKEIKIKGGSNADPHPLNDE 60

Query: 61  SHAQ-SCANQGCSASNVQCTPEIEKKYVHRVYDAIAPHFSSTRFAKWPKVASFLSSLPLG 120
           +HAQ SCA QGC+ASNVQCTPEIEKKYVHRVYDAIAPHFSSTRFAKWPKVASFLSSLPLG
Sbjct: 61  THAQLSCAIQGCTASNVQCTPEIEKKYVHRVYDAIAPHFSSTRFAKWPKVASFLSSLPLG 120

Query: 121 SLVLDAGCGNGKYLGYNPNCFFIGCDISAQLIKICNERGHEVLVADAVNLPYRTGFGDAA 180
           SLVLDAGCGNGKYLGYN NCFFIGCDISAQLIKICNERGHEVLVADAVNLPYRTGFGDAA
Sbjct: 121 SLVLDAGCGNGKYLGYNSNCFFIGCDISAQLIKICNERGHEVLVADAVNLPYRTGFGDAA 180

Query: 181 ISIAVLHHLSTENRRKKAIEELIRVVKKGGLVLITVWAVEQEDKSLLTKWMPLSEKYVEE 240
           ISIAVLHHLSTENRRKKAIEELIRVVKKGGLVLITVWAVEQEDKSLLTKWMPLSEKYVEE
Sbjct: 181 ISIAVLHHLSTENRRKKAIEELIRVVKKGGLVLITVWAVEQEDKSLLTKWMPLSEKYVEE 240

Query: 241 WVGPGSPRVRSPSSLALESIPEMNENNSGICLRDSKENVTGSIPENKPLPSQTENDLANC 300
           WVGPGSPRVRSPSS+ALESIPEMNENNSGICL+DSKEN+TGSIPENKP PSQ+ENDLANC
Sbjct: 241 WVGPGSPRVRSPSSMALESIPEMNENNSGICLKDSKENLTGSIPENKPPPSQSENDLANC 300

Query: 301 NDENLFKTQQEYFVPWHLPYHRAEVSGTSASALASGLAKKDDKKAAVVYNRYYHVFSEGE 360
           N ENL KTQQEYFVPWHLPYHRAEVSGTSASALASGLAKKDDKKAAVVYNRYYHVFSEGE
Sbjct: 301 NHENLLKTQQEYFVPWHLPYHRAEVSGTSASALASGLAKKDDKKAAVVYNRYYHVFSEGE 360

Query: 361 LERLISGMDNAVVVDRFYDKSNWCIVLEK 389
           LE LISGMDNAVVVDRFYDKSNWCIVLEK
Sbjct: 361 LEGLISGMDNAVVVDRFYDKSNWCIVLEK 389

[Close]
BLAST of Lsi03G004450 vs. NCBI nr
Match: gi|659081274|ref|XP_008441246.1| (PREDICTED: alkylated DNA repair protein alkB homolog 8 [Cucumis melo])

HSP 1 Score: 724.5 bits (1869), Expect = 1.1e-205
Identity = 359/389 (92.29%), Postives = 370/389 (95.12%), Query Frame = 1 

Query: 1   MPQAFDRASRLSKRFSHNPQIFGLSSVVSGSSHHFRRSSAMKEIKVKGGSNADPHLFNDE 60
           M Q+FD ASR+SK F H+ +I GLSSVVSGS HHFR +S MKEIKVKGGSNADPH  NDE
Sbjct: 1   MLQSFDCASRVSKHFCHSCKILGLSSVVSGSGHHFRLNSTMKEIKVKGGSNADPHPLNDE 60

Query: 61  SHAQ-SCANQGCSASNVQCTPEIEKKYVHRVYDAIAPHFSSTRFAKWPKVASFLSSLPLG 120
           +H Q SCA QGCSASNVQCTPEIEKKYVHRVYDAIAPHFSSTR+AKWPKVASFLSSLPLG
Sbjct: 61  THTQLSCAIQGCSASNVQCTPEIEKKYVHRVYDAIAPHFSSTRYAKWPKVASFLSSLPLG 120

Query: 121 SLVLDAGCGNGKYLGYNPNCFFIGCDISAQLIKICNERGHEVLVADAVNLPYRTGFGDAA 180
           SLVLDAGCGNGKYLGYNPNC FIGCDISAQLIKICNERGHEVLVADAVNLPYRTGFGDAA
Sbjct: 121 SLVLDAGCGNGKYLGYNPNCSFIGCDISAQLIKICNERGHEVLVADAVNLPYRTGFGDAA 180

Query: 181 ISIAVLHHLSTENRRKKAIEELIRVVKKGGLVLITVWAVEQEDKSLLTKWMPLSEKYVEE 240
           ISIAVLHHLSTENRRKKAIEELIRVVKKGGLVLITVWAVEQEDKSLLTKWMPLSEKYVEE
Sbjct: 181 ISIAVLHHLSTENRRKKAIEELIRVVKKGGLVLITVWAVEQEDKSLLTKWMPLSEKYVEE 240

Query: 241 WVGPGSPRVRSPSSLALESIPEMNENNSGICLRDSKENVTGSIPENKPLPSQTENDLANC 300
           WVGPGSPRVRSPSS+ALESIPEMNENNSGICLRDSKEN+TGSIPENKPL SQ+ENDLANC
Sbjct: 241 WVGPGSPRVRSPSSMALESIPEMNENNSGICLRDSKENLTGSIPENKPLHSQSENDLANC 300

Query: 301 NDENLFKTQQEYFVPWHLPYHRAEVSGTSASALASGLAKKDDKKAAVVYNRYYHVFSEGE 360
           N ENL KTQQEYFVPWHLPYHRAEVSGTSASALASGLAKKDDKKAAVVY+RYYHVFSEGE
Sbjct: 301 NHENLLKTQQEYFVPWHLPYHRAEVSGTSASALASGLAKKDDKKAAVVYSRYYHVFSEGE 360

Query: 361 LERLISGMDNAVVVDRFYDKSNWCIVLEK 389
           LE LISGMDNAVVVDRFYDKSNWCIVLEK
Sbjct: 361 LEGLISGMDNAVVVDRFYDKSNWCIVLEK 389

[Close]
BLAST of Lsi03G004450 vs. NCBI nr
Match: gi|802634662|ref|XP_012078118.1| (PREDICTED: alkylated DNA repair protein alkB homolog 8 [Jatropha curcas])

HSP 1 Score: 546.6 bits (1407), Expect = 4.1e-152
Identity = 277/390 (71.03%), Postives = 311/390 (79.74%), Query Frame = 1 

Query: 5   FDRASRLSKRFSHNPQIFGLSSVVSGSSHHFRRSSAMKEIKVKGGSNADPHLFNDESHAQ 64
           F R  RLSK F    + F LS +  GS  H +    M++IKVKG S    H F+ ES  Q
Sbjct: 5   FSRVGRLSKTFL---EAFTLSILAVGSVCHSQIFRNMRKIKVKGSSGLFSHAFDGESQIQ 64

Query: 65  S----CANQGCSASNVQCTPEIEKKYVHRVYDAIAPHFSSTRFAKWPKVASFLSSLPLGS 124
                C  Q C + +VQ TPEIE+KYVHRVYDAIAPHFSSTRFAKWPKVA+FL+SLP GS
Sbjct: 65  QSVSECEEQKCLSCSVQSTPEIERKYVHRVYDAIAPHFSSTRFAKWPKVATFLNSLPSGS 124

Query: 125 LVLDAGCGNGKYLGYNPNCFFIGCDISAQLIKICNERGHEVLVADAVNLPYRTGFGDAAI 184
           L+LDAGCGNGKYLG NP+CFFIGCDISA LIKIC +RGHEVLV DAVNLPYRTGFGDAAI
Sbjct: 125 LILDAGCGNGKYLGLNPDCFFIGCDISAPLIKICADRGHEVLVGDAVNLPYRTGFGDAAI 184

Query: 185 SIAVLHHLSTENRRKKAIEELIRVVKKGGLVLITVWAVEQEDKSLLTKWMPLSEKYVEEW 244
           SIAVLHHLSTENRRKKAIEEL+RVVKKGGLVLITVWAVEQED SL+TKW PL+++Y+EEW
Sbjct: 185 SIAVLHHLSTENRRKKAIEELVRVVKKGGLVLITVWAVEQEDGSLVTKWTPLTQRYLEEW 244

Query: 245 VGPGSPRVRSPSSLALESIPEMNENNSGICLRDSKENVTGSIPENKPLPSQTENDLANCN 304
           +GPGSPRVRSPSS+ LESIPE+ ENNS   ++DSKE     + +   L SQTE D     
Sbjct: 245 IGPGSPRVRSPSSITLESIPEIEENNSKDHVKDSKEKADKELKKMTHLTSQTEMDGIG-K 304

Query: 305 DENLFKTQQEYFVPWHLPYHRAEVSGTSASALASGLAKKDDKKAAVVYNRYYHVFSEGEL 364
           DE     QQEYFVPWHLPYHRAEVSG SA AL SGLAKKDDKK AV+YNRYYHVFSEGEL
Sbjct: 305 DEKNANNQQEYFVPWHLPYHRAEVSGASACALESGLAKKDDKKGAVIYNRYYHVFSEGEL 364

Query: 365 ERLISGMDNAVVVDRFYDKSNWCIVLEKTA 391
           +RL+ GM+N VVVDRF+DKSNWCI+LEKT+
Sbjct: 365 DRLVIGMNNVVVVDRFFDKSNWCIILEKTS 390

[Close]
BLAST of Lsi03G004450 vs. NCBI nr
Match: gi|643723114|gb|KDP32719.1| (hypothetical protein JCGZ_12011 [Jatropha curcas])

HSP 1 Score: 535.4 bits (1378), Expect = 9.4e-149
Identity = 264/354 (74.58%), Postives = 295/354 (83.33%), Query Frame = 1 

Query: 41  MKEIKVKGGSNADPHLFNDESHAQS----CANQGCSASNVQCTPEIEKKYVHRVYDAIAP 100
           M++IKVKG S    H F+ ES  Q     C  Q C + +VQ TPEIE+KYVHRVYDAIAP
Sbjct: 1   MRKIKVKGSSGLFSHAFDGESQIQQSVSECEEQKCLSCSVQSTPEIERKYVHRVYDAIAP 60

Query: 101 HFSSTRFAKWPKVASFLSSLPLGSLVLDAGCGNGKYLGYNPNCFFIGCDISAQLIKICNE 160
           HFSSTRFAKWPKVA+FL+SLP GSL+LDAGCGNGKYLG NP+CFFIGCDISA LIKIC +
Sbjct: 61  HFSSTRFAKWPKVATFLNSLPSGSLILDAGCGNGKYLGLNPDCFFIGCDISAPLIKICAD 120

Query: 161 RGHEVLVADAVNLPYRTGFGDAAISIAVLHHLSTENRRKKAIEELIRVVKKGGLVLITVW 220
           RGHEVLV DAVNLPYRTGFGDAAISIAVLHHLSTENRRKKAIEEL+RVVKKGGLVLITVW
Sbjct: 121 RGHEVLVGDAVNLPYRTGFGDAAISIAVLHHLSTENRRKKAIEELVRVVKKGGLVLITVW 180

Query: 221 AVEQEDKSLLTKWMPLSEKYVEEWVGPGSPRVRSPSSLALESIPEMNENNSGICLRDSKE 280
           AVEQED SL+TKW PL+++Y+EEW+GPGSPRVRSPSS+ LESIPE+ ENNS   ++DSKE
Sbjct: 181 AVEQEDGSLVTKWTPLTQRYLEEWIGPGSPRVRSPSSITLESIPEIEENNSKDHVKDSKE 240

Query: 281 NVTGSIPENKPLPSQTENDLANCNDENLFKTQQEYFVPWHLPYHRAEVSGTSASALASGL 340
                + +   L SQTE D     DE     QQEYFVPWHLPYHRAEVSG SA AL SGL
Sbjct: 241 KADKELKKMTHLTSQTEMDGIG-KDEKNANNQQEYFVPWHLPYHRAEVSGASACALESGL 300

Query: 341 AKKDDKKAAVVYNRYYHVFSEGELERLISGMDNAVVVDRFYDKSNWCIVLEKTA 391
           AKKDDKK AV+YNRYYHVFSEGEL+RL+ GM+N VVVDRF+DKSNWCI+LEKT+
Sbjct: 301 AKKDDKKGAVIYNRYYHVFSEGELDRLVIGMNNVVVVDRFFDKSNWCIILEKTS 353

[Close]
BLAST of Lsi03G004450 vs. NCBI nr
Match: gi|255545596|ref|XP_002513858.1| (PREDICTED: alkylated DNA repair protein alkB homolog 8 [Ricinus communis])

HSP 1 Score: 534.3 bits (1375), Expect = 2.1e-148
Identity = 276/388 (71.13%), Postives = 308/388 (79.38%), Query Frame = 1 

Query: 7   RASRLSKRFSHNPQIFGLSSVVSGSSHHFRRSSAMKEIKVKGGSNADPHLFNDESHAQSC 66
           RASR S+ +    Q F    +V G    F    AM+EIKVKG S       + ES  Q  
Sbjct: 7   RASRFSETYR---QAFARPIIVIGRVCLFGIFRAMREIKVKGPSGLSNVASDGESQIQLS 66

Query: 67  AN----QGCSASNVQCTPEIEKKYVHRVYDAIAPHFSSTRFAKWPKVASFLSSLPLGSLV 126
            +    Q CS+S+VQ TPEIEKKYVH VYDAIAPHFSSTRFAKWPKVA+FL+SLP GSL+
Sbjct: 67  LSADEKQVCSSSSVQSTPEIEKKYVHHVYDAIAPHFSSTRFAKWPKVATFLNSLPSGSLI 126

Query: 127 LDAGCGNGKYLGYNPNCFFIGCDISAQLIKICNERGHEVLVADAVNLPYRTGFGDAAISI 186
           LDAGCGNGKYLG NP+C+FIGCDISA LIKIC +RGHEVLV DAVNLPYRTGF DAAISI
Sbjct: 127 LDAGCGNGKYLGLNPDCYFIGCDISAPLIKICADRGHEVLVGDAVNLPYRTGFSDAAISI 186

Query: 187 AVLHHLSTENRRKKAIEELIRVVKKGGLVLITVWAVEQEDKSLLTKWMPLSEKYVEEWVG 246
           AVLHHLSTENRRKKAIEEL+RVVKKGGLVLITVWAVEQED SL+ KW PLS+KYVEEW+G
Sbjct: 187 AVLHHLSTENRRKKAIEELVRVVKKGGLVLITVWAVEQEDGSLVAKWTPLSQKYVEEWIG 246

Query: 247 PGSPRVRSPSSLALESIPEMNENNSGICLRDSKENVTGSIPENKPLPSQTENDLANCNDE 306
           PGSPRVRSPSS  LESIPE  ENNS   ++DS++N      +     SQTE++ +   DE
Sbjct: 247 PGSPRVRSPSSFTLESIPETEENNSKELIQDSEKNADEEFMKTMDSISQTEHN-SVFKDE 306

Query: 307 NLFKTQQEYFVPWHLPYHRAEVSGTSASALASGLAKKDDKKAAVVYNRYYHVFSEGELER 366
              K QQEYFVPWHLPYHRAEVSG SA ALASGLAKKDDKK AVVYNRYYHVFSEGELER
Sbjct: 307 KNAKNQQEYFVPWHLPYHRAEVSGASACALASGLAKKDDKKGAVVYNRYYHVFSEGELER 366

Query: 367 LISGMDNAVVVDRFYDKSNWCIVLEKTA 391
           L++GM NAVVVDRF+DKSNWC++LEKT+
Sbjct: 367 LVTGMKNAVVVDRFFDKSNWCVILEKTS 390

The following BLAST results are available for this feature:
BLAST of Lsi03G004450 vs. Swiss-Prot
Match NameE-valueIdentityDescription
ALKB8_MOUSE2.7e-5538.66Alkylated DNA repair protein alkB homolog 8 OS=Mus musculus GN=Alkbh8 PE=1 SV=1[more]
ALKB8_BOVIN2.3e-5437.54Alkylated DNA repair protein alkB homolog 8 OS=Bos taurus GN=ALKBH8 PE=2 SV=1[more]
ALKB8_MACFA4.3e-5335.74Alkylated DNA repair protein alkB homolog 8 OS=Macaca fascicularis GN=ALKBH8 PE=... [more]
ALKB8_HUMAN4.8e-5236.59Alkylated DNA repair protein alkB homolog 8 OS=Homo sapiens GN=ALKBH8 PE=1 SV=2[more]
ALKB8_XENTR1.9e-4836.94Alkylated DNA repair protein alkB homolog 8 OS=Xenopus tropicalis GN=alkbh8 PE=2... [more]
BLAST of Lsi03G004450 vs. TrEMBL
Match NameE-valueIdentityDescription
A0A0A0LMT8_CUCSA1.8e-20792.54Uncharacterized protein OS=Cucumis sativus GN=Csa_2G403730 PE=4 SV=1[more]
A0A067KLS3_JATCU6.6e-14974.58Uncharacterized protein OS=Jatropha curcas GN=JCGZ_12011 PE=4 SV=1[more]
B9RJD9_RICCO1.5e-14871.13S-adenosylmethionine-dependent methyltransferase, putative OS=Ricinus communis G... [more]
M5XBE8_PRUPE1.7e-14474.37Uncharacterized protein OS=Prunus persica GN=PRUPE_ppa007908mg PE=4 SV=1[more]
V4SHY6_9ROSI8.3e-14472.19Uncharacterized protein OS=Citrus clementina GN=CICLE_v10001407mg PE=4 SV=1[more]
BLAST of Lsi03G004450 vs. TAIR10
Match NameE-valueIdentityDescription
AT1G36310.19.1e-13467.20 S-adenosyl-L-methionine-dependent methyltransferases superfamily pro... [more]
BLAST of Lsi03G004450 vs. NCBI nr
Match NameE-valueIdentityDescription
gi|449441742|ref|XP_004138641.1|2.6e-20792.54PREDICTED: alkylated DNA repair protein alkB homolog 8 [Cucumis sativus][more]
gi|659081274|ref|XP_008441246.1|1.1e-20592.29PREDICTED: alkylated DNA repair protein alkB homolog 8 [Cucumis melo][more]
gi|802634662|ref|XP_012078118.1|4.1e-15271.03PREDICTED: alkylated DNA repair protein alkB homolog 8 [Jatropha curcas][more]
gi|643723114|gb|KDP32719.1|9.4e-14974.58hypothetical protein JCGZ_12011 [Jatropha curcas][more]
gi|255545596|ref|XP_002513858.1|2.1e-14871.13PREDICTED: alkylated DNA repair protein alkB homolog 8 [Ricinus communis][more]
Annotated Terms
The following terms have been associated with this gene:
Vocabulary: Molecular Function
TermDefinition
GO:0008168methyltransferase activity
Vocabulary: Biological Process
TermDefinition
GO:0008152metabolic process
Vocabulary: INTERPRO
TermDefinition
IPR013216Methyltransf_11
GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0030488 tRNA methylation
biological_process GO:0002098 tRNA wobble uridine modification
biological_process GO:0008152 metabolic process
cellular_component GO:0005575 cellular_component
molecular_function GO:0008175 tRNA methyltransferase activity
molecular_function GO:0008168 methyltransferase activity
molecular_function GO:0016300 tRNA (uracil) methyltransferase activity
Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Lsi03G004450.1Lsi03G004450.1mRNA


InterPro
Analysis Name: InterPro Annotations of Lagenaria siceraria
Date Performed: 2017-09-18
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR013216Methyltransferase type 11PFAMPF08241Methyltransf_11coord: 123..213
score: 3.7
NoneNo IPR availablePANTHERPTHR13069UNCHARACTERIZEDcoord: 79..227
score: 2.2E-113coord: 306..389
score: 2.2E
NoneNo IPR availablePANTHERPTHR13069:SF21PROTEIN C35D10.12coord: 79..227
score: 2.2E-113coord: 306..389
score: 2.2E