Lsi03G003840 (gene) Bottle gourd (USVL1VR-Ls) | Cucurbit Genomics Database (CuGenDB)

Overview


Name	Lsi03G003840
Type	gene
Organism	Lagenaria siceraria (Bottle gourd (USVL1VR-Ls))
Description	EF hand calcium-binding family protein
Location	chr03 : 4487367 .. 4488298 (+)

Genome Viewer

Gene Expression

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: five_prime_UTRCDS

Hold the cursor over a type above to highlight its positions in the sequence below.

AAGCAATTGAAGAAGGAAGAAAGATTGAAACTTTTTGAAAGGAATGAATCAAAAGAACTTTTCGCACTTTTATTATTTTATGTTTTCGCAATAACAAGTTGCCATTTGCCAGCCGTTTAAGACAGAGAAATGGACTCTTTCTTTTCACGTTCTACATGATTCTTCTTTTTCTTCTTCTTCTTCTTCTTCTTCTTCTTCTTCTTCATATAATTAGGGCTTTTTCCACGTTTTCTAGACTCTTTGATTCATTTATTAATCCCACCAACATTTTCTACGCACAATTGTTTATTCAACAATCGACTTCATTGAAACGGTGAAGGAAAAGTAATGGTCGACCATCCACTCCACCAGTTGACGGCAACTCCCATTTTCATTTACCAACTTCATCCCCAAAGCCGGCGGTCAAACCACGGTACGCGCATTCCCTTTCATATAATTATGCACACCCATTTGGCCCTAATCCCCAATTCTTCAACCTAATTTCTCTTCCTTCACTCGGATTCAGAGTCATGGACAAAATCGCATTGTACGAGAAGGTGTTCAAGCAGGTCGACGGTGACGGAGACGGGAAGCTGTCTCCGCCGGAGCTGCAGCAGTGTATGGCGGGCGTCGGAGGGAGTTTGACGCTCGAGGAGGCAGAGACGGTGGTTGAGAATTTGGATTCGGACGGCGATGGGTTAGTGGGGTGGGATGAATTTGTTGCATTTGTAAAAGAAGTGGGTGAGGAAGAGAAAGTGAATGATTTGAAGGAGGCGTTTAGAATGTATGAAATGGATGGTTGTGGGTTCATAACGACGAAGAGTTTGAAGAGGATGTTGAGTAAGCTTGGGGAATCAAGAAGTATTGAGGAATGCAAGAAAATGATTGCTAAATTTGATCTCAATTCTGATGGTGTTCTTAACTTTGATGAGTTCAAGTTCATGATGTCCTGA

mRNA sequence

AAGCAATTGAAGAAGGAAGAAAGATTGAAACTTTTTGAAAGGAATGAATCAAAAGAACTTTTCGCACTTTTATTATTTTATGTTTTCGCAATAACAAGTTGCCATTTGCCAGCCGTTTAAGACAGAGAAATGGACTCTTTCTTTTCACGTTCTACATGATTCTTCTTTTTCTTCTTCTTCTTCTTCTTCTTCTTCTTCTTCTTCATATAATTAGGGCTTTTTCCACGTTTTCTAGACTCTTTGATTCATTTATTAATCCCACCAACATTTTCTACGCACAATTGTTTATTCAACAATCGACTTCATTGAAACGGTGAAGGAAAAGTAATGGTCGACCATCCACTCCACCAGTTGACGGCAACTCCCATTTTCATTTACCAACTTCATCCCCAAAGCCGGCGGTCAAACCACGAGTCATGGACAAAATCGCATTGTACGAGAAGGTGTTCAAGCAGGTCGACGGTGACGGAGACGGGAAGCTGTCTCCGCCGGAGCTGCAGCAGTGTATGGCGGGCGTCGGAGGGAGTTTGACGCTCGAGGAGGCAGAGACGGTGGTTGAGAATTTGGATTCGGACGGCGATGGGTTAGTGGGGTGGGATGAATTTGTTGCATTTGTAAAAGAAGTGGGTGAGGAAGAGAAAGTGAATGATTTGAAGGAGGCGTTTAGAATGTATGAAATGGATGGTTGTGGGTTCATAACGACGAAGAGTTTGAAGAGGATGTTGAGTAAGCTTGGGGAATCAAGAAGTATTGAGGAATGCAAGAAAATGATTGCTAAATTTGATCTCAATTCTGATGGTGTTCTTAACTTTGATGAGTTCAAGTTCATGATGTCCTGA

Coding sequence (CDS)

ATGGACAAAATCGCATTGTACGAGAAGGTGTTCAAGCAGGTCGACGGTGACGGAGACGGGAAGCTGTCTCCGCCGGAGCTGCAGCAGTGTATGGCGGGCGTCGGAGGGAGTTTGACGCTCGAGGAGGCAGAGACGGTGGTTGAGAATTTGGATTCGGACGGCGATGGGTTAGTGGGGTGGGATGAATTTGTTGCATTTGTAAAAGAAGTGGGTGAGGAAGAGAAAGTGAATGATTTGAAGGAGGCGTTTAGAATGTATGAAATGGATGGTTGTGGGTTCATAACGACGAAGAGTTTGAAGAGGATGTTGAGTAAGCTTGGGGAATCAAGAAGTATTGAGGAATGCAAGAAAATGATTGCTAAATTTGATCTCAATTCTGATGGTGTTCTTAACTTTGATGAGTTCAAGTTCATGATGTCCTGA

Protein sequence

MDKIALYEKVFKQVDGDGDGKLSPPELQQCMAGVGGSLTLEEAETVVENLDSDGDGLVGWDEFVAFVKEVGEEEKVNDLKEAFRMYEMDGCGFITTKSLKRMLSKLGESRSIEECKKMIAKFDLNSDGVLNFDEFKFMMS

Homology

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BLAST of Lsi03G003840 vs. Swiss-Prot
Match: CML19_ORYSJ (Putative calcium-binding protein CML19 OS=Oryza sativa subsp. japonica GN=CML19 PE=3 SV=1)

HSP 1 Score: 133.3 bits (334), Expect = 2.1e-30
Identity = 74/139 (53.24%), Postives = 97/139 (69.78%), Query Frame = 1


Query: 5   ALYEKVFKQVDGDGDGKLSPPELQQCM-AGVGGSLTLEEAETVVENLDSDGDGLVGWDEF 64
           A + +VF   D D DGK+S  EL+ CM A +G  ++ EEAE +V + D+D DGL+  +EF
Sbjct: 6   AEFRRVFSAFDRDADGKISAAELRLCMKAALGEDMSAEEAEALVSSADTDDDGLLDEEEF 65

Query: 65  --VAFVKEVG-EEEKVNDLKEAFRMYEMDGCGFITTKSLKRMLSKLGESRSIEECKKMIA 124
             +A   E+G EEE+   L EAFRMYEM+G G IT  SLKRMLSKLG  + IEEC+ MI 
Sbjct: 66  TKLAVQLEMGDEEERCRGLMEAFRMYEMEGEGRITPASLKRMLSKLGSHQGIEECQTMIC 125

Query: 125 KFDLNSDGVLNFDEFKFMM 140
           +FDL+ DGV++F+EFK MM
Sbjct: 126 RFDLDGDGVISFEEFKIMM 144

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BLAST of Lsi03G003840 vs. Swiss-Prot
Match: CML38_ARATH (Calcium-binding protein CML38 OS=Arabidopsis thaliana GN=CML38 PE=2 SV=1)

HSP 1 Score: 128.3 bits (321), Expect = 6.8e-29
Identity = 68/138 (49.28%), Postives = 92/138 (66.67%), Query Frame = 1


Query: 2   DKIALYEKVFKQVDGDGDGKLSPPELQQCMAGVGGSLTLEEAETVVENLDSDGDGLVGWD 61
           DK    E VF  +D + DG++SP ELQ+    +G  L+ EEA   V   D+DGDG++ ++
Sbjct: 39  DKNRELEAVFSYMDANRDGRISPEELQKSFMTLGEQLSDEEAVAAVRLSDTDGDGMLDFE 98

Query: 62  EFVAFVKEVGEEEKVNDLKEAFRMYEMDGCGFITTKSLKRMLSKLGESRSIEECKKMIAK 121
           EF   +K   EEEK  +LK AFR+Y  +G   IT +SLK ML KLGESR+ ++C+ MI+ 
Sbjct: 99  EFSQLIKVDDEEEKKMELKGAFRLYIAEGEDCITPRSLKMMLKKLGESRTTDDCRVMISA 158

Query: 122 FDLNSDGVLNFDEFKFMM 140
           FDLN+DGVL+FDEF  MM
Sbjct: 159 FDLNADGVLSFDEFALMM 176

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BLAST of Lsi03G003840 vs. Swiss-Prot
Match: CML37_ARATH (Calcium-binding protein CML37 OS=Arabidopsis thaliana GN=CML37 PE=2 SV=1)

HSP 1 Score: 124.8 bits (312), Expect = 7.5e-28
Identity = 65/132 (49.24%), Postives = 91/132 (68.94%), Query Frame = 1


Query: 10  VFKQVDGDGDGKLSPPELQQCMAGVGGSLTLEEAETVVENLDSDGDGLVGWDEFVAFVK- 69
           VF  +D + DGK+S  ELQ C++ +GG+L+  E E VV+  D DGDG + ++EF+  ++ 
Sbjct: 53  VFDYMDANSDGKISGEELQSCVSLLGGALSSREVEEVVKTSDVDGDGFIDFEEFLKLMEG 112

Query: 70  -EVGEEEKVNDLKEAFRMYEMDGCGFITTKSLKRMLSKLGESRSIEECKKMIAKFDLNSD 129
            +  +EE+  +LKEAF MY M+G  FIT  SL+R LS+LGES +++ CK MI  FD N D
Sbjct: 113 EDGSDEERRKELKEAFGMYVMEGEEFITAASLRRTLSRLGESCTVDACKVMIRGFDQNDD 172

Query: 130 GVLNFDEFKFMM 140
           GVL+FDEF  MM
Sbjct: 173 GVLSFDEFVLMM 184

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BLAST of Lsi03G003840 vs. Swiss-Prot
Match: CML23_ORYSJ (Putative calcium-binding protein CML23 OS=Oryza sativa subsp. japonica GN=CML23 PE=3 SV=1)

HSP 1 Score: 122.9 bits (307), Expect = 2.9e-27
Identity = 70/143 (48.95%), Postives = 93/143 (65.03%), Query Frame = 1


Query: 7   YEKVFKQVDGDGDGKLSPPELQQCM-AGVGGSLTLEEAETVVENLDSDGDGLVGWDEFVA 66
           + +VF   D DGDGK+S  EL+ C+ A +G  +  EE + ++   D+DGDGL+  +EFV 
Sbjct: 7   FRRVFGSFDQDGDGKISATELRLCVKASLGEDMPDEEVQALMALADTDGDGLLDEEEFVR 66

Query: 67  FVKEV---GEEEKVND------LKEAFRMYEMDGCGFITTKSLKRMLSKLGESRSIEECK 126
            V E+   G+EE+ +D      L+EAF MYEM+G G IT  SLK MLSKLG    + EC+
Sbjct: 67  LVTEMEADGDEEEDDDDETCRCLREAFAMYEMEGRGCITPLSLKLMLSKLGTHLDVAECQ 126

Query: 127 KMIAKFDLNSDGVLNFDEFKFMM 140
            MI +FD+N DGVL FDEFK MM
Sbjct: 127 AMICRFDMNGDGVLTFDEFKTMM 149

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BLAST of Lsi03G003840 vs. Swiss-Prot
Match: CML31_ORYSJ (Probable calcium-binding protein CML31 OS=Oryza sativa subsp. japonica GN=CML31 PE=2 SV=1)

HSP 1 Score: 120.6 bits (301), Expect = 1.4e-26
Identity = 68/136 (50.00%), Postives = 94/136 (69.12%), Query Frame = 1


Query: 10  VFKQVDGDGDGKLSPPELQQCM-AGVGGSLTLEEAETVVENLDSDGDGLVGWDEFVAFVK 69
           +F   D DGDG++S  EL+ CM   +G  ++ EEA  +V ++D+DGDGL+   EFV  V+
Sbjct: 15  LFATFDHDGDGRISAAELRLCMKTTLGEEVSDEEAGQLVASVDADGDGLLCEAEFVRLVQ 74

Query: 70  --EVGEEE--KVNDLKEAFRMYEMDGCGFITTKSLKRMLSKLGESRSIEECKKMIAKFDL 129
             EV EE+  +   L+EAF MYEM+G G IT  SL+RML +LG  + I++C+ MI +FDL
Sbjct: 75  AAEVEEEDERRGTGLREAFGMYEMEGEGCITPTSLRRMLRRLGSDQDIDDCRAMICRFDL 134

Query: 130 NSDGVLNFDEFKFMMS 141
           N DGVL+FDEFK MM+
Sbjct: 135 NGDGVLSFDEFKIMMN 150

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BLAST of Lsi03G003840 vs. TrEMBL
Match: A0A0A0LMM5_CUCSA (Uncharacterized protein OS=Cucumis sativus GN=Csa_2G406780 PE=4 SV=1)

HSP 1 Score: 262.7 bits (670), Expect = 2.6e-67
Identity = 129/140 (92.14%), Postives = 136/140 (97.14%), Query Frame = 1


Query: 1   MDKIALYEKVFKQVDGDGDGKLSPPELQQCMAGVGGSLTLEEAETVVENLDSDGDGLVGW 60
           MDKIALYEKVFKQVDGDGDGKLSPPELQ+C+ GVGGSLT+EEAETVVE LDSDGDGLVGW
Sbjct: 1   MDKIALYEKVFKQVDGDGDGKLSPPELQRCILGVGGSLTIEEAETVVEKLDSDGDGLVGW 60

Query: 61  DEFVAFVKEVGEEEKVNDLKEAFRMYEMDGCGFITTKSLKRMLSKLGESRSIEECKKMIA 120
           DEFV FV+ VGEEEKVNDLKEAFRMYEMDGCGFITTKSLKRMLSKLGESRSI++CKKMIA
Sbjct: 61  DEFVEFVEGVGEEEKVNDLKEAFRMYEMDGCGFITTKSLKRMLSKLGESRSIDDCKKMIA 120

Query: 121 KFDLNSDGVLNFDEFKFMMS 141
           KFDL+SDGVLNFDEFKFMMS
Sbjct: 121 KFDLDSDGVLNFDEFKFMMS 140

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BLAST of Lsi03G003840 vs. TrEMBL
Match: A0A0D2VX70_GOSRA (Uncharacterized protein OS=Gossypium raimondii GN=B456_012G087800 PE=4 SV=1)

HSP 1 Score: 198.7 bits (504), Expect = 4.6e-48
Identity = 96/139 (69.06%), Postives = 115/139 (82.73%), Query Frame = 1


Query: 1   MDKIALYEKVFKQVDGDGDGKLSPPELQQCMAGVGGSLTLEEAETVVENLDSDGDGLVGW 60
           MDK   YE+VF   D + DGK+SP ELQQC+  +GG L+ EEAE  VE LD+DGDGL+G 
Sbjct: 1   MDKHQEYERVFNHFDENKDGKISPAELQQCVKAIGGELSREEAEVAVEVLDTDGDGLLGL 60

Query: 61  DEFVAFVKEVGEEEKVNDLKEAFRMYEMDGCGFITTKSLKRMLSKLGESRSIEECKKMIA 120
           ++F+  V+EVGEEEKVNDLKEAF+MYEM+GCG IT KSLKRMLS+LGESR++EECK MIA
Sbjct: 61  EDFIRLVEEVGEEEKVNDLKEAFKMYEMEGCGCITPKSLKRMLSRLGESRTLEECKSMIA 120

Query: 121 KFDLNSDGVLNFDEFKFMM 140
           +FDLN DGVLNFDEF+ MM
Sbjct: 121 QFDLNGDGVLNFDEFRVMM 139

[Close]

BLAST of Lsi03G003840 vs. TrEMBL
Match: V4U8A2_9ROSI (Uncharacterized protein OS=Citrus clementina GN=CICLE_v10018267mg PE=4 SV=1)

HSP 1 Score: 191.4 bits (485), Expect = 7.3e-46
Identity = 92/139 (66.19%), Postives = 112/139 (80.58%), Query Frame = 1


Query: 2   DKIALYEKVFKQVDGDGDGKLSPPELQQCMAGVGGSLTLEEAETVVENLDSDGDGLVGWD 61
           +K   YE+VF   D +GD K+SP E+QQC+  +GG L+L EAE  VE LD+DGDGL+G+D
Sbjct: 3   EKYQQYERVFNHFDSNGDRKISPSEIQQCVEAIGGELSLAEAEAAVEFLDADGDGLLGFD 62

Query: 62  EFVAFVKEVGEEEKVNDLKEAFRMYEMDGCGFITTKSLKRMLSKLGESRSIEECKKMIAK 121
           +FV  V+  GEEEK+NDLKEAF+MYEMDGCG IT KSLKRMLS+LG+S+S +ECK MIA 
Sbjct: 63  DFVRLVEGSGEEEKMNDLKEAFKMYEMDGCGCITPKSLKRMLSRLGQSKSDDECKSMIAY 122

Query: 122 FDLNSDGVLNFDEFKFMMS 141
           FDLN DGVLNFDEF+ MMS
Sbjct: 123 FDLNGDGVLNFDEFRIMMS 141

[Close]

BLAST of Lsi03G003840 vs. TrEMBL
Match: A0A067EP25_CITSI (Uncharacterized protein OS=Citrus sinensis GN=CISIN_1g037913mg PE=4 SV=1)

HSP 1 Score: 190.3 bits (482), Expect = 1.6e-45
Identity = 92/139 (66.19%), Postives = 112/139 (80.58%), Query Frame = 1


Query: 2   DKIALYEKVFKQVDGDGDGKLSPPELQQCMAGVGGSLTLEEAETVVENLDSDGDGLVGWD 61
           +K   YE+VF   D +GD ++SP ELQQC+  +GG L+L EAE  VE LD+DGDGL+G+D
Sbjct: 3   EKHQQYERVFNHFDSNGDRRISPSELQQCVEAIGGELSLAEAEAAVEFLDADGDGLLGFD 62

Query: 62  EFVAFVKEVGEEEKVNDLKEAFRMYEMDGCGFITTKSLKRMLSKLGESRSIEECKKMIAK 121
           +FV  V+  GEEEK+NDLKEAF+MYEMDGCG IT KSLKRMLS+LG+S+S +ECK MIA 
Sbjct: 63  DFVRLVEGSGEEEKMNDLKEAFKMYEMDGCGCITPKSLKRMLSRLGQSKSDDECKSMIAY 122

Query: 122 FDLNSDGVLNFDEFKFMMS 141
           FDLN DGVLNFDEF+ MMS
Sbjct: 123 FDLNGDGVLNFDEFRIMMS 141

[Close]

BLAST of Lsi03G003840 vs. TrEMBL
Match: M5WW87_PRUPE (Uncharacterized protein OS=Prunus persica GN=PRUPE_ppa025431mg PE=4 SV=1)

HSP 1 Score: 189.9 bits (481), Expect = 2.1e-45
Identity = 97/142 (68.31%), Postives = 114/142 (80.28%), Query Frame = 1


Query: 1   MDKIALYEKVFKQVDGDGDGKLSPPELQQCMAGVGGSLTLEEAETVVENLDSDGDGLVGW 60
           MDK+A YE+VF   D +GDGK+SP ELQQC+  +GG L+L EAE  VE+LDSDGDGL+  
Sbjct: 1   MDKVAQYERVFNHFDANGDGKISPLELQQCVRAIGGELSLTEAEVAVEHLDSDGDGLLCL 60

Query: 61  DEFVAFVKEVGEEEKVNDLKEAFRMYEM-DGCGF--ITTKSLKRMLSKLGESRSIEECKK 120
           D+FV FV    EEEKVNDLKEAF MY M DGCGF  IT KSLKRMLS+LGES+S+++CK 
Sbjct: 61  DDFVKFVDGGREEEKVNDLKEAFNMYVMDDGCGFSCITPKSLKRMLSRLGESKSVDDCKV 120

Query: 121 MIAKFDLNSDGVLNFDEFKFMM 140
           MI++FDLN DGVLNFDEFK MM
Sbjct: 121 MISRFDLNGDGVLNFDEFKVMM 142

[Close]

BLAST of Lsi03G003840 vs. TAIR10
Match: AT1G76650.1 (AT1G76650.1 calmodulin-like 38)

HSP 1 Score: 128.3 bits (321), Expect = 3.8e-30
Identity = 68/138 (49.28%), Postives = 92/138 (66.67%), Query Frame = 1


Query: 2   DKIALYEKVFKQVDGDGDGKLSPPELQQCMAGVGGSLTLEEAETVVENLDSDGDGLVGWD 61
           DK    E VF  +D + DG++SP ELQ+    +G  L+ EEA   V   D+DGDG++ ++
Sbjct: 39  DKNRELEAVFSYMDANRDGRISPEELQKSFMTLGEQLSDEEAVAAVRLSDTDGDGMLDFE 98

Query: 62  EFVAFVKEVGEEEKVNDLKEAFRMYEMDGCGFITTKSLKRMLSKLGESRSIEECKKMIAK 121
           EF   +K   EEEK  +LK AFR+Y  +G   IT +SLK ML KLGESR+ ++C+ MI+ 
Sbjct: 99  EFSQLIKVDDEEEKKMELKGAFRLYIAEGEDCITPRSLKMMLKKLGESRTTDDCRVMISA 158

Query: 122 FDLNSDGVLNFDEFKFMM 140
           FDLN+DGVL+FDEF  MM
Sbjct: 159 FDLNADGVLSFDEFALMM 176

[Close]

BLAST of Lsi03G003840 vs. TAIR10
Match: AT5G42380.1 (AT5G42380.1 calmodulin like 37)

HSP 1 Score: 124.8 bits (312), Expect = 4.2e-29
Identity = 65/132 (49.24%), Postives = 91/132 (68.94%), Query Frame = 1


Query: 10  VFKQVDGDGDGKLSPPELQQCMAGVGGSLTLEEAETVVENLDSDGDGLVGWDEFVAFVK- 69
           VF  +D + DGK+S  ELQ C++ +GG+L+  E E VV+  D DGDG + ++EF+  ++ 
Sbjct: 53  VFDYMDANSDGKISGEELQSCVSLLGGALSSREVEEVVKTSDVDGDGFIDFEEFLKLMEG 112

Query: 70  -EVGEEEKVNDLKEAFRMYEMDGCGFITTKSLKRMLSKLGESRSIEECKKMIAKFDLNSD 129
            +  +EE+  +LKEAF MY M+G  FIT  SL+R LS+LGES +++ CK MI  FD N D
Sbjct: 113 EDGSDEERRKELKEAFGMYVMEGEEFITAASLRRTLSRLGESCTVDACKVMIRGFDQNDD 172

Query: 130 GVLNFDEFKFMM 140
           GVL+FDEF  MM
Sbjct: 173 GVLSFDEFVLMM 184

[Close]

BLAST of Lsi03G003840 vs. TAIR10
Match: AT1G76640.1 (AT1G76640.1 Calcium-binding EF-hand family protein)

HSP 1 Score: 119.4 bits (298), Expect = 1.8e-27
Identity = 67/135 (49.63%), Postives = 87/135 (64.44%), Query Frame = 1


Query: 8   EKVFKQVDGDGDGKLSPPELQQCMAGVGGSLTLEEAETVVENLDSDGDGLVGWDEFVAFV 67
           E VF  +D + DG++S  EL++    +G  ++ EEAE  V+  D DGDG++  +EF   +
Sbjct: 24  EAVFAYMDANRDGRISAEELKKSFKTLGEQMSDEEAEAAVKLSDIDGDGMLDINEFALLI 83

Query: 68  K---EVGEEEKVNDLKEAFRMYEMDGCGFITTKSLKRMLSKLGESRSIEECKKMIAKFDL 127
           K   E  EEEK   + EAFRMY  DG   IT  SLK ML KLGESR+ ++CK MI  FDL
Sbjct: 84  KGNDEFTEEEKKRKIMEAFRMYIADGEDCITPGSLKMMLMKLGESRTTDDCKVMIQAFDL 143

Query: 128 NSDGVLNFDEFKFMM 140
           N+DGVL+FDEF  MM
Sbjct: 144 NADGVLSFDEFALMM 158

[Close]

BLAST of Lsi03G003840 vs. TAIR10
Match: AT5G37770.1 (AT5G37770.1 EF hand calcium-binding protein family)

HSP 1 Score: 116.3 bits (290), Expect = 1.5e-26
Identity = 60/139 (43.17%), Postives = 92/139 (66.19%), Query Frame = 1


Query: 8   EKVFKQVDGDGDGKLSPPELQQCMAGVGGSLTLEEAETVVENLDSDGDGLVGWDEFVAFV 67
           +KVF++ D +GDGK+S  EL++ +  +  + + EE  T+++  D DG+G +  DEFVA  
Sbjct: 19  KKVFQRFDKNGDGKISVDELKEVIRALSPTASPEETVTMMKQFDLDGNGFIDLDEFVALF 78

Query: 68  KEVG------EEEKVNDLKEAFRMYEMDGCGFITTKSLKRMLSKLGESRSIEECKKMIAK 127
            ++G          V+DLKEAF +Y++DG G I+ K L  ++  LGE  S+++CKKMI+K
Sbjct: 79  -QIGIGGGGNNRNDVSDLKEAFELYDLDGNGRISAKELHSVMKNLGEKCSVQDCKKMISK 138

Query: 128 FDLNSDGVLNFDEFKFMMS 141
            D++ DG +NFDEFK MMS
Sbjct: 139 VDIDGDGCVNFDEFKKMMS 156

[Close]

BLAST of Lsi03G003840 vs. TAIR10
Match: AT1G24620.1 (AT1G24620.1 EF hand calcium-binding protein family)

HSP 1 Score: 105.9 bits (263), Expect = 2.0e-23
Identity = 54/134 (40.30%), Postives = 83/134 (61.94%), Query Frame = 1


Query: 8   EKVFKQVDGDGDGKLSPPELQQCMAGVGGSLTLEEAETVVENLDSDGDGLVGWDEFVAF- 67
           E VFK+ D +GDGK+S  EL   M  +G  +  EE E  +  +D  GDG + ++EFV   
Sbjct: 39  EAVFKKFDVNGDGKISSKELGAIMTSLGHEVPEEELEKAITEIDRKGDGYINFEEFVELN 98

Query: 68  VKEVGEEEKVNDLKEAFRMYEMDGCGFITTKSLKRMLSKLGESRSIEECKKMIAKFDLNS 127
            K + + + + +LK+AF +Y++DG G I+ + L  +L  LG+  SI EC+KMI   D + 
Sbjct: 99  TKGMDQNDVLENLKDAFSVYDIDGNGSISAEELHEVLRSLGDECSIAECRKMIGGVDKDG 158

Query: 128 DGVLNFDEFKFMMS 141
           DG ++F+EFK MM+
Sbjct: 159 DGTIDFEEFKIMMT 172

[Close]

BLAST of Lsi03G003840 vs. NCBI nr
Match: gi|449441684|ref|XP_004138612.1| (PREDICTED: calcium-binding protein CML38-like [Cucumis sativus])

HSP 1 Score: 262.7 bits (670), Expect = 3.7e-67
Identity = 129/140 (92.14%), Postives = 136/140 (97.14%), Query Frame = 1


Query: 1   MDKIALYEKVFKQVDGDGDGKLSPPELQQCMAGVGGSLTLEEAETVVENLDSDGDGLVGW 60
           MDKIALYEKVFKQVDGDGDGKLSPPELQ+C+ GVGGSLT+EEAETVVE LDSDGDGLVGW
Sbjct: 1   MDKIALYEKVFKQVDGDGDGKLSPPELQRCILGVGGSLTIEEAETVVEKLDSDGDGLVGW 60

Query: 61  DEFVAFVKEVGEEEKVNDLKEAFRMYEMDGCGFITTKSLKRMLSKLGESRSIEECKKMIA 120
           DEFV FV+ VGEEEKVNDLKEAFRMYEMDGCGFITTKSLKRMLSKLGESRSI++CKKMIA
Sbjct: 61  DEFVEFVEGVGEEEKVNDLKEAFRMYEMDGCGFITTKSLKRMLSKLGESRSIDDCKKMIA 120

Query: 121 KFDLNSDGVLNFDEFKFMMS 141
           KFDL+SDGVLNFDEFKFMMS
Sbjct: 121 KFDLDSDGVLNFDEFKFMMS 140

[Close]

BLAST of Lsi03G003840 vs. NCBI nr
Match: gi|659081481|ref|XP_008441354.1| (PREDICTED: putative calcium-binding protein CML19 [Cucumis melo])

HSP 1 Score: 261.9 bits (668), Expect = 6.3e-67
Identity = 129/140 (92.14%), Postives = 136/140 (97.14%), Query Frame = 1


Query: 1   MDKIALYEKVFKQVDGDGDGKLSPPELQQCMAGVGGSLTLEEAETVVENLDSDGDGLVGW 60
           MDKIALYEKVFKQVDGDGDGKLSPPELQ+C+ GVGGSLT+EEAETVVE LDS+GDGLVGW
Sbjct: 1   MDKIALYEKVFKQVDGDGDGKLSPPELQRCILGVGGSLTIEEAETVVEKLDSNGDGLVGW 60

Query: 61  DEFVAFVKEVGEEEKVNDLKEAFRMYEMDGCGFITTKSLKRMLSKLGESRSIEECKKMIA 120
           DEFV FV+ VGEEEKVNDLKEAFRMYEMDGCGFITTKSLKRMLSKLGESRSIE+CKKMIA
Sbjct: 61  DEFVEFVEGVGEEEKVNDLKEAFRMYEMDGCGFITTKSLKRMLSKLGESRSIEDCKKMIA 120

Query: 121 KFDLNSDGVLNFDEFKFMMS 141
           KFDL+SDGVLNFDEFKFMMS
Sbjct: 121 KFDLDSDGVLNFDEFKFMMS 140

[Close]

BLAST of Lsi03G003840 vs. NCBI nr
Match: gi|764612590|ref|XP_011467704.1| (PREDICTED: calcium-binding protein CML38 [Fragaria vesca subsp. vesca])

HSP 1 Score: 203.8 bits (517), Expect = 2.0e-49
Identity = 99/139 (71.22%), Postives = 116/139 (83.45%), Query Frame = 1


Query: 1   MDKIALYEKVFKQVDGDGDGKLSPPELQQCMAGVGGSLTLEEAETVVENLDSDGDGLVGW 60
           MDK+A YE+VF Q DG+GDGK+SP ELQQC+  +GG L+  EAE  VE LDSD DGL+G+
Sbjct: 1   MDKLAQYERVFNQFDGNGDGKISPMELQQCVEAIGGELSSSEAEAAVEYLDSDRDGLLGF 60

Query: 61  DEFVAFVKEVGEEEKVNDLKEAFRMYEMDGCGFITTKSLKRMLSKLGESRSIEECKKMIA 120
           D+FV FV+   EEEKVNDL+EAF+MY+ DGCG IT KSLKRMLS+LGESRS++ECK MIA
Sbjct: 61  DDFVKFVEGGDEEEKVNDLREAFKMYDTDGCGCITPKSLKRMLSRLGESRSVDECKVMIA 120

Query: 121 KFDLNSDGVLNFDEFKFMM 140
           KFDLN DGVLNFDEFK MM
Sbjct: 121 KFDLNGDGVLNFDEFKVMM 139

[Close]

BLAST of Lsi03G003840 vs. NCBI nr
Match: gi|823251670|ref|XP_012458473.1| (PREDICTED: calcium-binding protein CML38-like [Gossypium raimondii])

HSP 1 Score: 198.7 bits (504), Expect = 6.5e-48
Identity = 96/139 (69.06%), Postives = 115/139 (82.73%), Query Frame = 1


Query: 1   MDKIALYEKVFKQVDGDGDGKLSPPELQQCMAGVGGSLTLEEAETVVENLDSDGDGLVGW 60
           MDK   YE+VF   D + DGK+SP ELQQC+  +GG L+ EEAE  VE LD+DGDGL+G 
Sbjct: 1   MDKHQEYERVFNHFDENKDGKISPAELQQCVKAIGGELSREEAEVAVEVLDTDGDGLLGL 60

Query: 61  DEFVAFVKEVGEEEKVNDLKEAFRMYEMDGCGFITTKSLKRMLSKLGESRSIEECKKMIA 120
           ++F+  V+EVGEEEKVNDLKEAF+MYEM+GCG IT KSLKRMLS+LGESR++EECK MIA
Sbjct: 61  EDFIRLVEEVGEEEKVNDLKEAFKMYEMEGCGCITPKSLKRMLSRLGESRTLEECKSMIA 120

Query: 121 KFDLNSDGVLNFDEFKFMM 140
           +FDLN DGVLNFDEF+ MM
Sbjct: 121 QFDLNGDGVLNFDEFRVMM 139

[Close]

BLAST of Lsi03G003840 vs. NCBI nr
Match: gi|658012114|ref|XP_008341320.1| (PREDICTED: putative calcium-binding protein CML19 [Malus domestica])

HSP 1 Score: 198.4 bits (503), Expect = 8.5e-48
Identity = 98/139 (70.50%), Postives = 115/139 (82.73%), Query Frame = 1


Query: 1   MDKIALYEKVFKQVDGDGDGKLSPPELQQCMAGVGGSLTLEEAETVVENLDSDGDGLVGW 60
           MDK+A YE+VFKQ DG+GDGK+SP ELQQC+  +GG L+L EAE  VE LDSDGDGL+G 
Sbjct: 1   MDKVAQYERVFKQFDGNGDGKISPIELQQCVGAIGGELSLTEAEVAVEYLDSDGDGLLGL 60

Query: 61  DEFVAFVKEVGEEEKVNDLKEAFRMYEMDGCGFITTKSLKRMLSKLGESRSIEECKKMIA 120
           D+FV FV    +EEKV+ LKEAF+MY MDG G IT KSLKRMLS+LGES+S++ECK MIA
Sbjct: 61  DDFVKFVDGGCDEEKVSVLKEAFKMYVMDGSGCITPKSLKRMLSRLGESKSVDECKNMIA 120

Query: 121 KFDLNSDGVLNFDEFKFMM 140
           +FDLN DGVLNFDEFK MM
Sbjct: 121 RFDLNGDGVLNFDEFKVMM 139

The following BLAST results are available for this feature:

BLAST of Lsi03G003840 vs. Swiss-Prot


Match Name	E-value	Identity	Description
CML19_ORYSJ	2.1e-30	53.24	Putative calcium-binding protein CML19 OS=Oryza sativa subsp. japonica GN=CML19 ...	[more]
CML38_ARATH	6.8e-29	49.28	Calcium-binding protein CML38 OS=Arabidopsis thaliana GN=CML38 PE=2 SV=1	[more]
CML37_ARATH	7.5e-28	49.24	Calcium-binding protein CML37 OS=Arabidopsis thaliana GN=CML37 PE=2 SV=1	[more]
CML23_ORYSJ	2.9e-27	48.95	Putative calcium-binding protein CML23 OS=Oryza sativa subsp. japonica GN=CML23 ...	[more]
CML31_ORYSJ	1.4e-26	50.00	Probable calcium-binding protein CML31 OS=Oryza sativa subsp. japonica GN=CML31 ...	[more]

BLAST of Lsi03G003840 vs. TrEMBL


Match Name	E-value	Identity	Description
A0A0A0LMM5_CUCSA	2.6e-67	92.14	Uncharacterized protein OS=Cucumis sativus GN=Csa_2G406780 PE=4 SV=1	[more]
A0A0D2VX70_GOSRA	4.6e-48	69.06	Uncharacterized protein OS=Gossypium raimondii GN=B456_012G087800 PE=4 SV=1	[more]
V4U8A2_9ROSI	7.3e-46	66.19	Uncharacterized protein OS=Citrus clementina GN=CICLE_v10018267mg PE=4 SV=1	[more]
A0A067EP25_CITSI	1.6e-45	66.19	Uncharacterized protein OS=Citrus sinensis GN=CISIN_1g037913mg PE=4 SV=1	[more]
M5WW87_PRUPE	2.1e-45	68.31	Uncharacterized protein OS=Prunus persica GN=PRUPE_ppa025431mg PE=4 SV=1	[more]

BLAST of Lsi03G003840 vs. TAIR10


Match Name	E-value	Identity	Description
AT1G76650.1	3.8e-30	49.28	calmodulin-like 38	[more]
AT5G42380.1	4.2e-29	49.24	calmodulin like 37	[more]
AT1G76640.1	1.8e-27	49.63	Calcium-binding EF-hand family protein	[more]
AT5G37770.1	1.5e-26	43.17	EF hand calcium-binding protein family	[more]
AT1G24620.1	2.0e-23	40.30	EF hand calcium-binding protein family	[more]

BLAST of Lsi03G003840 vs. NCBI nr


Match Name	E-value	Identity	Description
gi\|449441684\|ref\|XP_004138612.1\|	3.7e-67	92.14	PREDICTED: calcium-binding protein CML38-like [Cucumis sativus]	[more]
gi\|659081481\|ref\|XP_008441354.1\|	6.3e-67	92.14	PREDICTED: putative calcium-binding protein CML19 [Cucumis melo]	[more]
gi\|764612590\|ref\|XP_011467704.1\|	2.0e-49	71.22	PREDICTED: calcium-binding protein CML38 [Fragaria vesca subsp. vesca]	[more]
gi\|823251670\|ref\|XP_012458473.1\|	6.5e-48	69.06	PREDICTED: calcium-binding protein CML38-like [Gossypium raimondii]	[more]
gi\|658012114\|ref\|XP_008341320.1\|	8.5e-48	70.50	PREDICTED: putative calcium-binding protein CML19 [Malus domestica]	[more]

Annotated Terms

The following terms have been associated with this gene:

Vocabulary: **Molecular Function**
Term	Definition
GO:0005509	calcium ion binding

Vocabulary: **INTERPRO**
Term	Definition
IPR018247	EF_Hand_1_Ca_BS
IPR011992	EF-hand-dom_pair
IPR002048	EF_hand_dom

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0008150	biological_process
biological_process	GO:0006612	protein targeting to membrane
biological_process	GO:0009862	systemic acquired resistance, salicylic acid mediated signaling pathway
biological_process	GO:0009697	salicylic acid biosynthetic process
biological_process	GO:0010200	response to chitin
biological_process	GO:0002679	respiratory burst involved in defense response
biological_process	GO:0010363	regulation of plant-type hypersensitive response
biological_process	GO:0010310	regulation of hydrogen peroxide metabolic process
biological_process	GO:0031348	negative regulation of defense response
biological_process	GO:0000165	MAPK cascade
biological_process	GO:0009867	jasmonic acid mediated signaling pathway
biological_process	GO:0009693	ethylene biosynthetic process
biological_process	GO:0030968	endoplasmic reticulum unfolded protein response
biological_process	GO:0050832	defense response to fungus
biological_process	GO:0042742	defense response to bacterium
cellular_component	GO:0005575	cellular_component
cellular_component	GO:0005622	intracellular
molecular_function	GO:0005509	calcium ion binding

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Lsi03G003840.1	Lsi03G003840.1	mRNA

InterPro

Analysis Name: InterPro Annotations of Lagenaria siceraria
Date Performed: 2017-09-18


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR002048	EF-hand domain	PFAM	PF13499	EF-hand_7	coord: 78..139 score: 3.2E-11coord: 6..67 score: 7.3
IPR002048	EF-hand domain	SMART	SM00054	efh_1	coord: 114..140 score: 0.26coord: 42..70 score: 6.3coord: 6..34 score: 0.01coord: 78..106 score:
IPR002048	EF-hand domain	PROFILE	PS50222	EF_HAND_2	coord: 110..140 score: 14.318coord: 2..37 score: 13.704coord: 74..109 score: 11.361coord: 38..73 score: 12
IPR011992	EF-hand domain pair	GENE3D	G3DSA:1.10.238.10		coord: 4..139 score: 2.0
IPR011992	EF-hand domain pair	unknown	SSF47473	EF-hand	coord: 4..139 score: 6.45
IPR018247	EF-Hand 1, calcium-binding site	PROSITE	PS00018	EF_HAND_1	coord: 123..135 score: -coord: 15..27 score: -coord: 51..63 scor
None	No IPR available	PANTHER	PTHR10891	EF-HAND CALCIUM-BINDING DOMAIN CONTAINING PROTEIN	coord: 7..139 score: 4.6
None	No IPR available	PANTHER	PTHR10891:SF621	SUBFAMILY NOT NAMED	coord: 7..139 score: 4.6

Synteny

The following gene(s) are orthologous to this gene:


Gene	Orthologue	Organism	Block
Lsi03G003840	Csa2G406780	Cucumber (Chinese Long) v2	culsiB115
Lsi03G003840	CSPI02G25720	Wild cucumber (PI 183967)	cpilsiB113
Lsi03G003840	Cucsa.087640	Cucumber (Gy14) v1	cgylsiB098
Lsi03G003840	CmaCh04G029650	Cucurbita maxima (Rimu)	cmalsiB656
Lsi03G003840	CmaCh15G000830	Cucurbita maxima (Rimu)	cmalsiB276
Lsi03G003840	CmoCh15G000840	Cucurbita moschata (Rifu)	cmolsiB265
Lsi03G003840	Cla006517	Watermelon (97103) v1	lsiwmB277
Lsi03G003840	MELO3C008204	Melon (DHL92) v3.5.1	lsimeB259
Lsi03G003840	ClCG11G006990	Watermelon (Charleston Gray)	lsiwcgB226
Lsi03G003840	MELO3C008204.2	Melon (DHL92) v3.6.1	lsimedB272
Lsi03G003840	Cla97C11G213930	Watermelon (97103) v2	lsiwmbB222
Lsi03G003840	Bhi04G001974	Wax gourd	lsiwgoB322
Lsi03G003840	Cp4.1LG13g11120	Cucurbita pepo (Zucchini)	cpelsiB143
Lsi03G003840	CsGy2G025350	Cucumber (Gy14) v2	cgyblsiB102
Lsi03G003840	Carg06040	Silver-seed gourd	carlsiB018
Lsi03G003840	Carg24272	Silver-seed gourd	carlsiB924
Lsi03G003840	CsaV3_2G034220	Cucumber (Chinese Long) v3	cuclsiB127

The following gene(s) are paralogous to this gene:

None

The following block(s) are covering this gene:


Gene	Organism	Block
Lsi03G003840	Cucurbita maxima (Rimu)	cmalsiB663
Lsi03G003840	Cucurbita moschata (Rifu)	cmolsiB645
Lsi03G003840	Cucurbita moschata (Rifu)	cmolsiB654
Lsi03G003840	Cucurbita pepo (Zucchini)	cpelsiB339
Lsi03G003840	Cucurbita pepo (Zucchini)	cpelsiB344
Lsi03G003840	Silver-seed gourd	carlsiB017