CmaCh04G029650 (gene) Cucurbita maxima (Rimu)

NameCmaCh04G029650
Typegene
OrganismCucurbita maxima (Cucurbita maxima (Rimu))
DescriptionCalcium binding protein
LocationCma_Chr04 : 19477567 .. 19478581 (-)
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: CDSexonthree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGTTTAAAAGGGGTAACATGGTAATTTCAATATTGTCAAAAGCCATCCCAAATCTGTTGAATAATACCCCTTTATTTGTGGAAATCACCCTTTTTAGTCTTCAGGTTTTTTTTACGATTCTTTAAACAAAATTTTCTTGGCGGAATTTTAGGGCATTTCCCACGTTTTCCGCCCTTCTCCGTCAATTGTTTATTCTTCAACAATCGCTGAAGAAGCCCACCTTCTTCAAATTCTAGAAATGGTCATCCACCTGCTCCACGAGTTGACGGCAACCACCATTTTCATTTGCCTACTTCTTCGCCGGTCAAACTGCGGTAACGTAACGCGCATTCCCTTTCATATAACTGCATACCCATGTTTCCCCAATTCTCCATTCTCATTTCTCCTAATTCACTCTCGCTCTCCACGCATTCAGAATTATGGACAAAATCGCATTGTACGAGAAGGTGTTCAAGCAGGTCGACGGCGACGGTGACGGGAAGCTATCTCTCCCGGAGTTGCAGCGGTGCATGGCGGTCGTCGGGGGTAGCTTGACACTCGAGGAGGCGGAGAAAGTGGTGGAGAATTTGGATTCCGACGGCGATGGGTTGGTGAGCTGGGCTGAATTTGTTGCGTTTATGGAAGAAGTAGGAGAGGAAGAGAAAGTGAGTGATTTGATGGAGGCGTTCAGAATGTATGAAATGGATGGCTGTGGATTCATAACCACTAAGAGCTTGAAGAGGATGCTGAGTAGGCTTGGGGAATCCAGGAGCATCGAGGAATGCAAGAAAATGATAGCTAAATTTGATCTCAATTCTGATGGTGTTCTTAACTTTGATGAGTTTAAGTTCATGATGTCCTGATTTTCTTCCATTTTTATGTGTTCTGAACTTAGATTTTTGGTCACCAGTTATATACGATTTACTCTTTCTCCTTGTGTTCTTCAATGGTTTGCATTCTCTCTCATAAACTAAAAACTAAGTATAAAATGAACGCTCATCAGCTTACTAAATTACATAAGAACTGCAAGTGAAG

mRNA sequence

ATGTTTAAAAGGGGTAACATGGGCATTTCCCACGTTTTCCGCCCTTCTCCAAATGGTCATCCACCTGCTCCACGAGTTGACGGCAACCACCATTTTCATTTGCCTACTTCTTCGCCGGTCAAACTGCGAATTATGGACAAAATCGCATTGTACGAGAAGGTGTTCAAGCAGGTCGACGGCGACGGTGACGGGAAGCTATCTCTCCCGGAGTTGCAGCGGTGCATGGCGGTCGTCGGGGGTAGCTTGACACTCGAGGAGGCGGAGAAAGTGGTGGAGAATTTGGATTCCGACGGCGATGGGTTGGTGAGCTGGGCTGAATTTGTTGCGTTTATGGAAGAAGTAGGAGAGGAAGAGAAAGTGAGTGATTTGATGGAGGCGTTCAGAATGTATGAAATGGATGGCTGTGGATTCATAACCACTAAGAGCTTGAAGAGGATGCTGAGTAGGCTTGGGGAATCCAGGAGCATCGAGGAATGCAAGAAAATGATAGCTAAATTTGATCTCAATTCTGATGGTGTTCTTAACTTTGATGAGTTTAAGTTCATGATGTCCTGATTTTCTTCCATTTTTATGTGTTCTGAACTTAGATTTTTGGTCACCAGTTATATACGATTTACTCTTTCTCCTTGTGTTCTTCAATGGTTTGCATTCTCTCTCATAAACTAAAAACTAAGTATAAAATGAACGCTCATCAGCTTACTAAATTACATAAGAACTGCAAGTGAAG

Coding sequence (CDS)

ATGTTTAAAAGGGGTAACATGGGCATTTCCCACGTTTTCCGCCCTTCTCCAAATGGTCATCCACCTGCTCCACGAGTTGACGGCAACCACCATTTTCATTTGCCTACTTCTTCGCCGGTCAAACTGCGAATTATGGACAAAATCGCATTGTACGAGAAGGTGTTCAAGCAGGTCGACGGCGACGGTGACGGGAAGCTATCTCTCCCGGAGTTGCAGCGGTGCATGGCGGTCGTCGGGGGTAGCTTGACACTCGAGGAGGCGGAGAAAGTGGTGGAGAATTTGGATTCCGACGGCGATGGGTTGGTGAGCTGGGCTGAATTTGTTGCGTTTATGGAAGAAGTAGGAGAGGAAGAGAAAGTGAGTGATTTGATGGAGGCGTTCAGAATGTATGAAATGGATGGCTGTGGATTCATAACCACTAAGAGCTTGAAGAGGATGCTGAGTAGGCTTGGGGAATCCAGGAGCATCGAGGAATGCAAGAAAATGATAGCTAAATTTGATCTCAATTCTGATGGTGTTCTTAACTTTGATGAGTTTAAGTTCATGATGTCCTGA

Protein sequence

MFKRGNMGISHVFRPSPNGHPPAPRVDGNHHFHLPTSSPVKLRIMDKIALYEKVFKQVDGDGDGKLSLPELQRCMAVVGGSLTLEEAEKVVENLDSDGDGLVSWAEFVAFMEEVGEEEKVSDLMEAFRMYEMDGCGFITTKSLKRMLSRLGESRSIEECKKMIAKFDLNSDGVLNFDEFKFMMS
BLAST of CmaCh04G029650 vs. Swiss-Prot
Match: CML19_ORYSJ (Putative calcium-binding protein CML19 OS=Oryza sativa subsp. japonica GN=CML19 PE=3 SV=1)

HSP 1 Score: 133.7 bits (335), Expect = 2.1e-30
Identity = 74/139 (53.24%), Postives = 97/139 (69.78%), Query Frame = 1

Query: 49  ALYEKVFKQVDGDGDGKLSLPELQRCM-AVVGGSLTLEEAEKVVENLDSDGDGLVSWAEF 108
           A + +VF   D D DGK+S  EL+ CM A +G  ++ EEAE +V + D+D DGL+   EF
Sbjct: 6   AEFRRVFSAFDRDADGKISAAELRLCMKAALGEDMSAEEAEALVSSADTDDDGLLDEEEF 65

Query: 109 --VAFMEEVG-EEEKVSDLMEAFRMYEMDGCGFITTKSLKRMLSRLGESRSIEECKKMIA 168
             +A   E+G EEE+   LMEAFRMYEM+G G IT  SLKRMLS+LG  + IEEC+ MI 
Sbjct: 66  TKLAVQLEMGDEEERCRGLMEAFRMYEMEGEGRITPASLKRMLSKLGSHQGIEECQTMIC 125

Query: 169 KFDLNSDGVLNFDEFKFMM 184
           +FDL+ DGV++F+EFK MM
Sbjct: 126 RFDLDGDGVISFEEFKIMM 144

BLAST of CmaCh04G029650 vs. Swiss-Prot
Match: CML37_ARATH (Calcium-binding protein CML37 OS=Arabidopsis thaliana GN=CML37 PE=2 SV=1)

HSP 1 Score: 128.3 bits (321), Expect = 8.9e-29
Identity = 67/132 (50.76%), Postives = 91/132 (68.94%), Query Frame = 1

Query: 54  VFKQVDGDGDGKLSLPELQRCMAVVGGSLTLEEAEKVVENLDSDGDGLVSWAEFVAFME- 113
           VF  +D + DGK+S  ELQ C++++GG+L+  E E+VV+  D DGDG + + EF+  ME 
Sbjct: 53  VFDYMDANSDGKISGEELQSCVSLLGGALSSREVEEVVKTSDVDGDGFIDFEEFLKLMEG 112

Query: 114 -EVGEEEKVSDLMEAFRMYEMDGCGFITTKSLKRMLSRLGESRSIEECKKMIAKFDLNSD 173
            +  +EE+  +L EAF MY M+G  FIT  SL+R LSRLGES +++ CK MI  FD N D
Sbjct: 113 EDGSDEERRKELKEAFGMYVMEGEEFITAASLRRTLSRLGESCTVDACKVMIRGFDQNDD 172

Query: 174 GVLNFDEFKFMM 184
           GVL+FDEF  MM
Sbjct: 173 GVLSFDEFVLMM 184

BLAST of CmaCh04G029650 vs. Swiss-Prot
Match: CML31_ORYSJ (Probable calcium-binding protein CML31 OS=Oryza sativa subsp. japonica GN=CML31 PE=2 SV=1)

HSP 1 Score: 125.6 bits (314), Expect = 5.8e-28
Identity = 69/136 (50.74%), Postives = 96/136 (70.59%), Query Frame = 1

Query: 54  VFKQVDGDGDGKLSLPELQRCM-AVVGGSLTLEEAEKVVENLDSDGDGLVSWAEFVAFME 113
           +F   D DGDG++S  EL+ CM   +G  ++ EEA ++V ++D+DGDGL+  AEFV  ++
Sbjct: 15  LFATFDHDGDGRISAAELRLCMKTTLGEEVSDEEAGQLVASVDADGDGLLCEAEFVRLVQ 74

Query: 114 --EVGEEE--KVSDLMEAFRMYEMDGCGFITTKSLKRMLSRLGESRSIEECKKMIAKFDL 173
             EV EE+  + + L EAF MYEM+G G IT  SL+RML RLG  + I++C+ MI +FDL
Sbjct: 75  AAEVEEEDERRGTGLREAFGMYEMEGEGCITPTSLRRMLRRLGSDQDIDDCRAMICRFDL 134

Query: 174 NSDGVLNFDEFKFMMS 185
           N DGVL+FDEFK MM+
Sbjct: 135 NGDGVLSFDEFKIMMN 150

BLAST of CmaCh04G029650 vs. Swiss-Prot
Match: CML38_ARATH (Calcium-binding protein CML38 OS=Arabidopsis thaliana GN=CML38 PE=2 SV=1)

HSP 1 Score: 119.4 bits (298), Expect = 4.1e-26
Identity = 64/138 (46.38%), Postives = 89/138 (64.49%), Query Frame = 1

Query: 46  DKIALYEKVFKQVDGDGDGKLSLPELQRCMAVVGGSLTLEEAEKVVENLDSDGDGLVSWA 105
           DK    E VF  +D + DG++S  ELQ+    +G  L+ EEA   V   D+DGDG++ + 
Sbjct: 39  DKNRELEAVFSYMDANRDGRISPEELQKSFMTLGEQLSDEEAVAAVRLSDTDGDGMLDFE 98

Query: 106 EFVAFMEEVGEEEKVSDLMEAFRMYEMDGCGFITTKSLKRMLSRLGESRSIEECKKMIAK 165
           EF   ++   EEEK  +L  AFR+Y  +G   IT +SLK ML +LGESR+ ++C+ MI+ 
Sbjct: 99  EFSQLIKVDDEEEKKMELKGAFRLYIAEGEDCITPRSLKMMLKKLGESRTTDDCRVMISA 158

Query: 166 FDLNSDGVLNFDEFKFMM 184
           FDLN+DGVL+FDEF  MM
Sbjct: 159 FDLNADGVLSFDEFALMM 176

BLAST of CmaCh04G029650 vs. Swiss-Prot
Match: CML39_ARATH (Calcium-binding protein CML39 OS=Arabidopsis thaliana GN=CML39 PE=2 SV=1)

HSP 1 Score: 119.0 bits (297), Expect = 5.4e-26
Identity = 66/135 (48.89%), Postives = 87/135 (64.44%), Query Frame = 1

Query: 52  EKVFKQVDGDGDGKLSLPELQRCMAVVGGSLTLEEAEKVVENLDSDGDGLVSWAEFVAFM 111
           E VF  +D + DG++S  EL++    +G  ++ EEAE  V+  D DGDG++   EF   +
Sbjct: 24  EAVFAYMDANRDGRISAEELKKSFKTLGEQMSDEEAEAAVKLSDIDGDGMLDINEFALLI 83

Query: 112 ---EEVGEEEKVSDLMEAFRMYEMDGCGFITTKSLKRMLSRLGESRSIEECKKMIAKFDL 171
              +E  EEEK   +MEAFRMY  DG   IT  SLK ML +LGESR+ ++CK MI  FDL
Sbjct: 84  KGNDEFTEEEKKRKIMEAFRMYIADGEDCITPGSLKMMLMKLGESRTTDDCKVMIQAFDL 143

Query: 172 NSDGVLNFDEFKFMM 184
           N+DGVL+FDEF  MM
Sbjct: 144 NADGVLSFDEFALMM 158

BLAST of CmaCh04G029650 vs. TrEMBL
Match: A0A0A0LMM5_CUCSA (Uncharacterized protein OS=Cucumis sativus GN=Csa_2G406780 PE=4 SV=1)

HSP 1 Score: 245.0 bits (624), Expect = 7.3e-62
Identity = 122/140 (87.14%), Postives = 130/140 (92.86%), Query Frame = 1

Query: 45  MDKIALYEKVFKQVDGDGDGKLSLPELQRCMAVVGGSLTLEEAEKVVENLDSDGDGLVSW 104
           MDKIALYEKVFKQVDGDGDGKLS PELQRC+  VGGSLT+EEAE VVE LDSDGDGLV W
Sbjct: 1   MDKIALYEKVFKQVDGDGDGKLSPPELQRCILGVGGSLTIEEAETVVEKLDSDGDGLVGW 60

Query: 105 AEFVAFMEEVGEEEKVSDLMEAFRMYEMDGCGFITTKSLKRMLSRLGESRSIEECKKMIA 164
            EFV F+E VGEEEKV+DL EAFRMYEMDGCGFITTKSLKRMLS+LGESRSI++CKKMIA
Sbjct: 61  DEFVEFVEGVGEEEKVNDLKEAFRMYEMDGCGFITTKSLKRMLSKLGESRSIDDCKKMIA 120

Query: 165 KFDLNSDGVLNFDEFKFMMS 185
           KFDL+SDGVLNFDEFKFMMS
Sbjct: 121 KFDLDSDGVLNFDEFKFMMS 140

BLAST of CmaCh04G029650 vs. TrEMBL
Match: A0A0D2VX70_GOSRA (Uncharacterized protein OS=Gossypium raimondii GN=B456_012G087800 PE=4 SV=1)

HSP 1 Score: 186.8 bits (473), Expect = 2.4e-44
Identity = 92/139 (66.19%), Postives = 111/139 (79.86%), Query Frame = 1

Query: 45  MDKIALYEKVFKQVDGDGDGKLSLPELQRCMAVVGGSLTLEEAEKVVENLDSDGDGLVSW 104
           MDK   YE+VF   D + DGK+S  ELQ+C+  +GG L+ EEAE  VE LD+DGDGL+  
Sbjct: 1   MDKHQEYERVFNHFDENKDGKISPAELQQCVKAIGGELSREEAEVAVEVLDTDGDGLLGL 60

Query: 105 AEFVAFMEEVGEEEKVSDLMEAFRMYEMDGCGFITTKSLKRMLSRLGESRSIEECKKMIA 164
            +F+  +EEVGEEEKV+DL EAF+MYEM+GCG IT KSLKRMLSRLGESR++EECK MIA
Sbjct: 61  EDFIRLVEEVGEEEKVNDLKEAFKMYEMEGCGCITPKSLKRMLSRLGESRTLEECKSMIA 120

Query: 165 KFDLNSDGVLNFDEFKFMM 184
           +FDLN DGVLNFDEF+ MM
Sbjct: 121 QFDLNGDGVLNFDEFRVMM 139

BLAST of CmaCh04G029650 vs. TrEMBL
Match: A5AHM0_VITVI (Putative uncharacterized protein OS=Vitis vinifera GN=VITISV_011291 PE=4 SV=1)

HSP 1 Score: 182.2 bits (461), Expect = 5.8e-43
Identity = 88/134 (65.67%), Postives = 108/134 (80.60%), Query Frame = 1

Query: 50  LYEKVFKQVDGDGDGKLSLPELQRCMAVVGGSLTLEEAEKVVENLDSDGDGLVSWAEFVA 109
           +YE++FK+ D DGDGKLS  EL+RC+  +G  L +EEA++VVE++DSDGDGL+   EFV 
Sbjct: 6   VYERIFKRFDEDGDGKLSPSELRRCLGTIGEELLMEEAQEVVESMDSDGDGLLGLEEFVG 65

Query: 110 FMEEVGEEEKVSDLMEAFRMYEMDGCGFITTKSLKRMLSRLGESRSIEECKKMIAKFDLN 169
           +ME  GEE K+ DL EAFRMYEM+G G IT KSLKRMLSRLGESRS+EEC  MIA+FD N
Sbjct: 66  WMEREGEERKMQDLREAFRMYEMEGSGCITPKSLKRMLSRLGESRSVEECSVMIAQFDXN 125

Query: 170 SDGVLNFDEFKFMM 184
            DGVL+FDEFK M+
Sbjct: 126 GDGVLSFDEFKLML 139

BLAST of CmaCh04G029650 vs. TrEMBL
Match: F6HG75_VITVI (Putative uncharacterized protein OS=Vitis vinifera GN=VIT_01s0010g03000 PE=4 SV=1)

HSP 1 Score: 181.0 bits (458), Expect = 1.3e-42
Identity = 88/139 (63.31%), Postives = 110/139 (79.14%), Query Frame = 1

Query: 45  MDKIALYEKVFKQVDGDGDGKLSLPELQRCMAVVGGSLTLEEAEKVVENLDSDGDGLVSW 104
           M   ++YE++FK+ D DGDGKLS  EL+RC+  +G  L +EEA++VVE++DSDGDGLV  
Sbjct: 1   MINCSIYERIFKRFDEDGDGKLSPSELRRCVGTIGEELLMEEAQEVVESMDSDGDGLVGL 60

Query: 105 AEFVAFMEEVGEEEKVSDLMEAFRMYEMDGCGFITTKSLKRMLSRLGESRSIEECKKMIA 164
            EFV +M+  GEE K+ +L EAFRMYEM+G G IT KSLKRMLSRLGESRS+EEC  MI 
Sbjct: 61  EEFVGWMKREGEERKMEELREAFRMYEMEGSGCITPKSLKRMLSRLGESRSVEECSVMIR 120

Query: 165 KFDLNSDGVLNFDEFKFMM 184
           +FD+N DGVL+FDEFK MM
Sbjct: 121 QFDVNGDGVLSFDEFKLMM 139

BLAST of CmaCh04G029650 vs. TrEMBL
Match: A0A151QQW8_CAJCA (Putative calcium-binding protein CML19 OS=Cajanus cajan GN=KK1_046572 PE=4 SV=1)

HSP 1 Score: 179.5 bits (454), Expect = 3.8e-42
Identity = 91/139 (65.47%), Postives = 109/139 (78.42%), Query Frame = 1

Query: 45  MDKIALYEKVFKQVDGDGDGKLSLPELQRCMAVVGGSLTLEEAEKVVENLDSDGDGLVSW 104
           MDK++ Y +VF   D +GDGK+S  EL++C+  VGG L+LEEAE VVE LDSD DGLV  
Sbjct: 1   MDKMSQYVRVFNHFDENGDGKISPSELRQCVEAVGGELSLEEAEAVVELLDSDKDGLVGL 60

Query: 105 AEFVAFMEEVGEEEKVSDLMEAFRMYEMDGCGFITTKSLKRMLSRLGESRSIEECKKMIA 164
            +FV F EE  EEEKV DL EAF+MYEM+GCG IT KSLKRML RLGE +SI+ECK MIA
Sbjct: 61  DDFVRFAEEGEEEEKVKDLREAFKMYEMEGCGCITPKSLKRMLGRLGECKSIDECKVMIA 120

Query: 165 KFDLNSDGVLNFDEFKFMM 184
           +FDL+ DGVL+FDEF+ MM
Sbjct: 121 RFDLDGDGVLSFDEFRVMM 139

BLAST of CmaCh04G029650 vs. TAIR10
Match: AT5G42380.1 (AT5G42380.1 calmodulin like 37)

HSP 1 Score: 128.3 bits (321), Expect = 5.0e-30
Identity = 67/132 (50.76%), Postives = 91/132 (68.94%), Query Frame = 1

Query: 54  VFKQVDGDGDGKLSLPELQRCMAVVGGSLTLEEAEKVVENLDSDGDGLVSWAEFVAFME- 113
           VF  +D + DGK+S  ELQ C++++GG+L+  E E+VV+  D DGDG + + EF+  ME 
Sbjct: 53  VFDYMDANSDGKISGEELQSCVSLLGGALSSREVEEVVKTSDVDGDGFIDFEEFLKLMEG 112

Query: 114 -EVGEEEKVSDLMEAFRMYEMDGCGFITTKSLKRMLSRLGESRSIEECKKMIAKFDLNSD 173
            +  +EE+  +L EAF MY M+G  FIT  SL+R LSRLGES +++ CK MI  FD N D
Sbjct: 113 EDGSDEERRKELKEAFGMYVMEGEEFITAASLRRTLSRLGESCTVDACKVMIRGFDQNDD 172

Query: 174 GVLNFDEFKFMM 184
           GVL+FDEF  MM
Sbjct: 173 GVLSFDEFVLMM 184

BLAST of CmaCh04G029650 vs. TAIR10
Match: AT1G76650.1 (AT1G76650.1 calmodulin-like 38)

HSP 1 Score: 119.4 bits (298), Expect = 2.3e-27
Identity = 64/138 (46.38%), Postives = 89/138 (64.49%), Query Frame = 1

Query: 46  DKIALYEKVFKQVDGDGDGKLSLPELQRCMAVVGGSLTLEEAEKVVENLDSDGDGLVSWA 105
           DK    E VF  +D + DG++S  ELQ+    +G  L+ EEA   V   D+DGDG++ + 
Sbjct: 39  DKNRELEAVFSYMDANRDGRISPEELQKSFMTLGEQLSDEEAVAAVRLSDTDGDGMLDFE 98

Query: 106 EFVAFMEEVGEEEKVSDLMEAFRMYEMDGCGFITTKSLKRMLSRLGESRSIEECKKMIAK 165
           EF   ++   EEEK  +L  AFR+Y  +G   IT +SLK ML +LGESR+ ++C+ MI+ 
Sbjct: 99  EFSQLIKVDDEEEKKMELKGAFRLYIAEGEDCITPRSLKMMLKKLGESRTTDDCRVMISA 158

Query: 166 FDLNSDGVLNFDEFKFMM 184
           FDLN+DGVL+FDEF  MM
Sbjct: 159 FDLNADGVLSFDEFALMM 176

BLAST of CmaCh04G029650 vs. TAIR10
Match: AT1G76640.1 (AT1G76640.1 Calcium-binding EF-hand family protein)

HSP 1 Score: 119.0 bits (297), Expect = 3.0e-27
Identity = 66/135 (48.89%), Postives = 87/135 (64.44%), Query Frame = 1

Query: 52  EKVFKQVDGDGDGKLSLPELQRCMAVVGGSLTLEEAEKVVENLDSDGDGLVSWAEFVAFM 111
           E VF  +D + DG++S  EL++    +G  ++ EEAE  V+  D DGDG++   EF   +
Sbjct: 24  EAVFAYMDANRDGRISAEELKKSFKTLGEQMSDEEAEAAVKLSDIDGDGMLDINEFALLI 83

Query: 112 ---EEVGEEEKVSDLMEAFRMYEMDGCGFITTKSLKRMLSRLGESRSIEECKKMIAKFDL 171
              +E  EEEK   +MEAFRMY  DG   IT  SLK ML +LGESR+ ++CK MI  FDL
Sbjct: 84  KGNDEFTEEEKKRKIMEAFRMYIADGEDCITPGSLKMMLMKLGESRTTDDCKVMIQAFDL 143

Query: 172 NSDGVLNFDEFKFMM 184
           N+DGVL+FDEF  MM
Sbjct: 144 NADGVLSFDEFALMM 158

BLAST of CmaCh04G029650 vs. TAIR10
Match: AT5G37770.1 (AT5G37770.1 EF hand calcium-binding protein family)

HSP 1 Score: 112.1 bits (279), Expect = 3.7e-25
Identity = 59/138 (42.75%), Postives = 89/138 (64.49%), Query Frame = 1

Query: 52  EKVFKQVDGDGDGKLSLPELQRCMAVVGGSLTLEEAEKVVENLDSDGDGLVSWAEFVA-F 111
           +KVF++ D +GDGK+S+ EL+  +  +  + + EE   +++  D DG+G +   EFVA F
Sbjct: 19  KKVFQRFDKNGDGKISVDELKEVIRALSPTASPEETVTMMKQFDLDGNGFIDLDEFVALF 78

Query: 112 MEEVG----EEEKVSDLMEAFRMYEMDGCGFITTKSLKRMLSRLGESRSIEECKKMIAKF 171
              +G        VSDL EAF +Y++DG G I+ K L  ++  LGE  S+++CKKMI+K 
Sbjct: 79  QIGIGGGGNNRNDVSDLKEAFELYDLDGNGRISAKELHSVMKNLGEKCSVQDCKKMISKV 138

Query: 172 DLNSDGVLNFDEFKFMMS 185
           D++ DG +NFDEFK MMS
Sbjct: 139 DIDGDGCVNFDEFKKMMS 156

BLAST of CmaCh04G029650 vs. TAIR10
Match: AT1G24620.1 (AT1G24620.1 EF hand calcium-binding protein family)

HSP 1 Score: 102.4 bits (254), Expect = 3.0e-22
Identity = 53/134 (39.55%), Postives = 83/134 (61.94%), Query Frame = 1

Query: 52  EKVFKQVDGDGDGKLSLPELQRCMAVVGGSLTLEEAEKVVENLDSDGDGLVSWAEFVAFM 111
           E VFK+ D +GDGK+S  EL   M  +G  +  EE EK +  +D  GDG +++ EFV   
Sbjct: 39  EAVFKKFDVNGDGKISSKELGAIMTSLGHEVPEEELEKAITEIDRKGDGYINFEEFVELN 98

Query: 112 EE-VGEEEKVSDLMEAFRMYEMDGCGFITTKSLKRMLSRLGESRSIEECKKMIAKFDLNS 171
            + + + + + +L +AF +Y++DG G I+ + L  +L  LG+  SI EC+KMI   D + 
Sbjct: 99  TKGMDQNDVLENLKDAFSVYDIDGNGSISAEELHEVLRSLGDECSIAECRKMIGGVDKDG 158

Query: 172 DGVLNFDEFKFMMS 185
           DG ++F+EFK MM+
Sbjct: 159 DGTIDFEEFKIMMT 172

BLAST of CmaCh04G029650 vs. NCBI nr
Match: gi|449441684|ref|XP_004138612.1| (PREDICTED: calcium-binding protein CML38-like [Cucumis sativus])

HSP 1 Score: 245.0 bits (624), Expect = 1.0e-61
Identity = 122/140 (87.14%), Postives = 130/140 (92.86%), Query Frame = 1

Query: 45  MDKIALYEKVFKQVDGDGDGKLSLPELQRCMAVVGGSLTLEEAEKVVENLDSDGDGLVSW 104
           MDKIALYEKVFKQVDGDGDGKLS PELQRC+  VGGSLT+EEAE VVE LDSDGDGLV W
Sbjct: 1   MDKIALYEKVFKQVDGDGDGKLSPPELQRCILGVGGSLTIEEAETVVEKLDSDGDGLVGW 60

Query: 105 AEFVAFMEEVGEEEKVSDLMEAFRMYEMDGCGFITTKSLKRMLSRLGESRSIEECKKMIA 164
            EFV F+E VGEEEKV+DL EAFRMYEMDGCGFITTKSLKRMLS+LGESRSI++CKKMIA
Sbjct: 61  DEFVEFVEGVGEEEKVNDLKEAFRMYEMDGCGFITTKSLKRMLSKLGESRSIDDCKKMIA 120

Query: 165 KFDLNSDGVLNFDEFKFMMS 185
           KFDL+SDGVLNFDEFKFMMS
Sbjct: 121 KFDLDSDGVLNFDEFKFMMS 140

BLAST of CmaCh04G029650 vs. NCBI nr
Match: gi|659081481|ref|XP_008441354.1| (PREDICTED: putative calcium-binding protein CML19 [Cucumis melo])

HSP 1 Score: 244.2 bits (622), Expect = 1.8e-61
Identity = 122/140 (87.14%), Postives = 130/140 (92.86%), Query Frame = 1

Query: 45  MDKIALYEKVFKQVDGDGDGKLSLPELQRCMAVVGGSLTLEEAEKVVENLDSDGDGLVSW 104
           MDKIALYEKVFKQVDGDGDGKLS PELQRC+  VGGSLT+EEAE VVE LDS+GDGLV W
Sbjct: 1   MDKIALYEKVFKQVDGDGDGKLSPPELQRCILGVGGSLTIEEAETVVEKLDSNGDGLVGW 60

Query: 105 AEFVAFMEEVGEEEKVSDLMEAFRMYEMDGCGFITTKSLKRMLSRLGESRSIEECKKMIA 164
            EFV F+E VGEEEKV+DL EAFRMYEMDGCGFITTKSLKRMLS+LGESRSIE+CKKMIA
Sbjct: 61  DEFVEFVEGVGEEEKVNDLKEAFRMYEMDGCGFITTKSLKRMLSKLGESRSIEDCKKMIA 120

Query: 165 KFDLNSDGVLNFDEFKFMMS 185
           KFDL+SDGVLNFDEFKFMMS
Sbjct: 121 KFDLDSDGVLNFDEFKFMMS 140

BLAST of CmaCh04G029650 vs. NCBI nr
Match: gi|764612590|ref|XP_011467704.1| (PREDICTED: calcium-binding protein CML38 [Fragaria vesca subsp. vesca])

HSP 1 Score: 191.4 bits (485), Expect = 1.4e-45
Identity = 95/139 (68.35%), Postives = 112/139 (80.58%), Query Frame = 1

Query: 45  MDKIALYEKVFKQVDGDGDGKLSLPELQRCMAVVGGSLTLEEAEKVVENLDSDGDGLVSW 104
           MDK+A YE+VF Q DG+GDGK+S  ELQ+C+  +GG L+  EAE  VE LDSD DGL+ +
Sbjct: 1   MDKLAQYERVFNQFDGNGDGKISPMELQQCVEAIGGELSSSEAEAAVEYLDSDRDGLLGF 60

Query: 105 AEFVAFMEEVGEEEKVSDLMEAFRMYEMDGCGFITTKSLKRMLSRLGESRSIEECKKMIA 164
            +FV F+E   EEEKV+DL EAF+MY+ DGCG IT KSLKRMLSRLGESRS++ECK MIA
Sbjct: 61  DDFVKFVEGGDEEEKVNDLREAFKMYDTDGCGCITPKSLKRMLSRLGESRSVDECKVMIA 120

Query: 165 KFDLNSDGVLNFDEFKFMM 184
           KFDLN DGVLNFDEFK MM
Sbjct: 121 KFDLNGDGVLNFDEFKVMM 139

BLAST of CmaCh04G029650 vs. NCBI nr
Match: gi|658012114|ref|XP_008341320.1| (PREDICTED: putative calcium-binding protein CML19 [Malus domestica])

HSP 1 Score: 187.2 bits (474), Expect = 2.6e-44
Identity = 94/139 (67.63%), Postives = 112/139 (80.58%), Query Frame = 1

Query: 45  MDKIALYEKVFKQVDGDGDGKLSLPELQRCMAVVGGSLTLEEAEKVVENLDSDGDGLVSW 104
           MDK+A YE+VFKQ DG+GDGK+S  ELQ+C+  +GG L+L EAE  VE LDSDGDGL+  
Sbjct: 1   MDKVAQYERVFKQFDGNGDGKISPIELQQCVGAIGGELSLTEAEVAVEYLDSDGDGLLGL 60

Query: 105 AEFVAFMEEVGEEEKVSDLMEAFRMYEMDGCGFITTKSLKRMLSRLGESRSIEECKKMIA 164
            +FV F++   +EEKVS L EAF+MY MDG G IT KSLKRMLSRLGES+S++ECK MIA
Sbjct: 61  DDFVKFVDGGCDEEKVSVLKEAFKMYVMDGSGCITPKSLKRMLSRLGESKSVDECKNMIA 120

Query: 165 KFDLNSDGVLNFDEFKFMM 184
           +FDLN DGVLNFDEFK MM
Sbjct: 121 RFDLNGDGVLNFDEFKVMM 139

BLAST of CmaCh04G029650 vs. NCBI nr
Match: gi|823251670|ref|XP_012458473.1| (PREDICTED: calcium-binding protein CML38-like [Gossypium raimondii])

HSP 1 Score: 186.8 bits (473), Expect = 3.4e-44
Identity = 92/139 (66.19%), Postives = 111/139 (79.86%), Query Frame = 1

Query: 45  MDKIALYEKVFKQVDGDGDGKLSLPELQRCMAVVGGSLTLEEAEKVVENLDSDGDGLVSW 104
           MDK   YE+VF   D + DGK+S  ELQ+C+  +GG L+ EEAE  VE LD+DGDGL+  
Sbjct: 1   MDKHQEYERVFNHFDENKDGKISPAELQQCVKAIGGELSREEAEVAVEVLDTDGDGLLGL 60

Query: 105 AEFVAFMEEVGEEEKVSDLMEAFRMYEMDGCGFITTKSLKRMLSRLGESRSIEECKKMIA 164
            +F+  +EEVGEEEKV+DL EAF+MYEM+GCG IT KSLKRMLSRLGESR++EECK MIA
Sbjct: 61  EDFIRLVEEVGEEEKVNDLKEAFKMYEMEGCGCITPKSLKRMLSRLGESRTLEECKSMIA 120

Query: 165 KFDLNSDGVLNFDEFKFMM 184
           +FDLN DGVLNFDEF+ MM
Sbjct: 121 QFDLNGDGVLNFDEFRVMM 139

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
CML19_ORYSJ2.1e-3053.24Putative calcium-binding protein CML19 OS=Oryza sativa subsp. japonica GN=CML19 ... [more]
CML37_ARATH8.9e-2950.76Calcium-binding protein CML37 OS=Arabidopsis thaliana GN=CML37 PE=2 SV=1[more]
CML31_ORYSJ5.8e-2850.74Probable calcium-binding protein CML31 OS=Oryza sativa subsp. japonica GN=CML31 ... [more]
CML38_ARATH4.1e-2646.38Calcium-binding protein CML38 OS=Arabidopsis thaliana GN=CML38 PE=2 SV=1[more]
CML39_ARATH5.4e-2648.89Calcium-binding protein CML39 OS=Arabidopsis thaliana GN=CML39 PE=2 SV=1[more]
Match NameE-valueIdentityDescription
A0A0A0LMM5_CUCSA7.3e-6287.14Uncharacterized protein OS=Cucumis sativus GN=Csa_2G406780 PE=4 SV=1[more]
A0A0D2VX70_GOSRA2.4e-4466.19Uncharacterized protein OS=Gossypium raimondii GN=B456_012G087800 PE=4 SV=1[more]
A5AHM0_VITVI5.8e-4365.67Putative uncharacterized protein OS=Vitis vinifera GN=VITISV_011291 PE=4 SV=1[more]
F6HG75_VITVI1.3e-4263.31Putative uncharacterized protein OS=Vitis vinifera GN=VIT_01s0010g03000 PE=4 SV=... [more]
A0A151QQW8_CAJCA3.8e-4265.47Putative calcium-binding protein CML19 OS=Cajanus cajan GN=KK1_046572 PE=4 SV=1[more]
Match NameE-valueIdentityDescription
AT5G42380.15.0e-3050.76 calmodulin like 37[more]
AT1G76650.12.3e-2746.38 calmodulin-like 38[more]
AT1G76640.13.0e-2748.89 Calcium-binding EF-hand family protein[more]
AT5G37770.13.7e-2542.75 EF hand calcium-binding protein family[more]
AT1G24620.13.0e-2239.55 EF hand calcium-binding protein family[more]
Match NameE-valueIdentityDescription
gi|449441684|ref|XP_004138612.1|1.0e-6187.14PREDICTED: calcium-binding protein CML38-like [Cucumis sativus][more]
gi|659081481|ref|XP_008441354.1|1.8e-6187.14PREDICTED: putative calcium-binding protein CML19 [Cucumis melo][more]
gi|764612590|ref|XP_011467704.1|1.4e-4568.35PREDICTED: calcium-binding protein CML38 [Fragaria vesca subsp. vesca][more]
gi|658012114|ref|XP_008341320.1|2.6e-4467.63PREDICTED: putative calcium-binding protein CML19 [Malus domestica][more]
gi|823251670|ref|XP_012458473.1|3.4e-4466.19PREDICTED: calcium-binding protein CML38-like [Gossypium raimondii][more]
The following terms have been associated with this gene:
Vocabulary: INTERPRO
TermDefinition
IPR002048EF_hand_dom
IPR011992EF-hand-dom_pair
IPR018247EF_Hand_1_Ca_BS
Vocabulary: Molecular Function
TermDefinition
GO:0005509calcium ion binding
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0008150 biological_process
biological_process GO:0006612 protein targeting to membrane
biological_process GO:0009862 systemic acquired resistance, salicylic acid mediated signaling pathway
biological_process GO:0009697 salicylic acid biosynthetic process
biological_process GO:0010200 response to chitin
biological_process GO:0002679 respiratory burst involved in defense response
biological_process GO:0010363 regulation of plant-type hypersensitive response
biological_process GO:0010310 regulation of hydrogen peroxide metabolic process
biological_process GO:0031348 negative regulation of defense response
biological_process GO:0000165 MAPK cascade
biological_process GO:0009867 jasmonic acid mediated signaling pathway
biological_process GO:0009693 ethylene biosynthetic process
biological_process GO:0030968 endoplasmic reticulum unfolded protein response
biological_process GO:0050832 defense response to fungus
biological_process GO:0042742 defense response to bacterium
cellular_component GO:0005575 cellular_component
cellular_component GO:0005622 intracellular
molecular_function GO:0005509 calcium ion binding

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
CmaCh04G029650.1CmaCh04G029650.1mRNA


Analysis Name: InterPro Annotations of Cucurbita maxima
Date Performed: 2017-05-20
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR002048EF-hand domainPFAMPF13499EF-hand_7coord: 50..112
score: 3.9E-13coord: 122..183
score: 4.4
IPR002048EF-hand domainSMARTSM00054efh_1coord: 158..184
score: 0.26coord: 86..114
score: 1.6coord: 122..150
score: 0.73coord: 50..78
score: 0
IPR002048EF-hand domainPROFILEPS50222EF_HAND_2coord: 118..153
score: 11.026coord: 82..117
score: 13.593coord: 46..81
score: 13.425coord: 154..184
score: 14
IPR011992EF-hand domain pairGENE3DG3DSA:1.10.238.10coord: 48..183
score: 1.1
IPR011992EF-hand domain pairunknownSSF47473EF-handcoord: 48..183
score: 7.19
IPR018247EF-Hand 1, calcium-binding sitePROSITEPS00018EF_HAND_1coord: 167..179
score: -coord: 59..71
score: -coord: 95..107
scor
NoneNo IPR availablePANTHERPTHR10891EF-HAND CALCIUM-BINDING DOMAIN CONTAINING PROTEINcoord: 52..184
score: 1.2
NoneNo IPR availablePANTHERPTHR10891:SF590SUBFAMILY NOT NAMEDcoord: 52..184
score: 1.2

The following gene(s) are paralogous to this gene:
GeneParalogueOrganismBlock
CmaCh04G029650CmaCh15G000830Cucurbita maxima (Rimu)cmacmaB320