Lsi03G000690 (gene) Bottle gourd (USVL1VR-Ls)

Overview
NameLsi03G000690
Typegene
OrganismLagenaria siceraria (Bottle gourd (USVL1VR-Ls))
DescriptionMagnesium transporter MRS2-like protein
Locationchr03 : 977826 .. 981470 (-)
Genome Viewer
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: five_prime_UTRCDS
Hold the cursor over a type above to highlight its positions in the sequence below.
TGGAAATTGATTTAATATAAAAAAAATACACCTATTTTTTTTTTCCCTTCTTCTTTCAAATCGAAGATCCCAAGAAGCTTAAAGCATACTTTTAATGTTCACAACCAGAGAGCTCGGATCGCTCTCCAATGGCGCTTTCTCTATCCCTACAACTTATCCGATTCCCTCCTCAAGCATTATCTTCATCCTCTCACCATCTTCACATCTCTGACCTTGGCTCCGTCTCGCGCTTCTTCCGATCTCCATGTCTTTCCTCTTTGACTCCGTCGCGGAGCTGCACGATGACGTTATCTTCCAGAACGAAATGTTTCTCCATGTCCACCGAGGAGGAGCAGTGGAGTGAACCGGAAGCGTTGGTGTCAGATGTGGACGGAGGGGGAGATCTTGAGGACGATATTGATATTGGTGTTCAGGAAGACAGCTCTGTGGCCACTGCTGCTTCTGCGCAGAGGATTTCTTCCTCGTCGCCGAGTGATTATCTGTCGCTTGCGATTCGAGAGCAGGTTTATGAGGTCTGTTGCCTTGATGCATTTTGTTTTATTTTGTTTTTCTTTTTCACGTGAATTCGGACGTGTTTGCTGCTAGTTTGGGTGCTGGATTGAAGTAGGAAATTTGAACATCGAACCAAAAATTTCAATCAAACTAATAGTAAGATTTCCTCGCATTTGATGTTAGGTTGCAAATGGTTTAATATTAAAATTTTAAACAGTAGTAAGTGCTTCTAATCTTGTAGTAATTTAATGGTTTCTAAATTGATTTTTAAACATAAAAGACATTAGCAACATAGAAACTTTTTTTTAGTTCATGAATTCAAATCTCTAACCTATTGGTCGAAGAAATGAGGTAACTTTATGCTTTAACTAGTTGAACTATATTAGGTTGGTGATCATACACCAACATTTAATTAGTTAGAGGATATAAATCATATAAAATATGCATAGTATAAATTATAGCCCTATCAAAGAAATGATACAAAATTATGACTTTTGAGGGGTTCAACTCCAGGTATTCACTCTTCTTTGAGGTCACAATTTTTTAATTATTTTTTTGGATATGACTACAAATCCTGTTTGAAGGGCAGAATGTTTACAAAATTTCAGCTCAACAAATTCTGTTTGAAGGGCAGAATGTTTACAAAATTTCAGGTCAACAAATTCTTTTTGAAGGGCAGAATACTTACAAAATTTCAGCTTAATTTAAGACCTTGTGTTATAATCCTTCCACTGATTGGAGTTAATGGTTTGGAGCTAGTTATCGTTTTGATTCCTCAATCAAATCTTTTCCTGTTAGAGATATCTCTGCGGGTTTCTTTCTTAGATAAAGTTCTTACACTCAATGTTCATCTCTATTGACCACTTAGGTGTTAGAGGTTAAAGCTGATGGAACCGTATCTACCAGGAAGGTTAACAGACGGCAACTATTGAAGTCAAGTGGTGAGTAAATATTACCAGAAATTTCAGCTTCTCATTTCAATGCGTAGTTTCTGGATGTATACCGTTGAAAACTTTTAGTCTGCATTGACTTGTGTATACTTTCTTTCGGAAATGTCGATTACTACTGGATGTCTCACGGTTGGTTTTTTGGATTCACCGATAGATAACCGTGGGATGACAAATAGTATGACTACATACTTGTTAACAAACTATATATTTTCCTCTTGTGGATATATATTTTTTTAATTATCAGGGCTTCGTCCACGTGATATCCGAAGTGTTGATCCATCTTTATTTTTGACAAACTCGATGCCTACATTACTGGTATATTGATTTTCACCTTTTTAATTCCCGGTCATATTCTTTTATATTATAAGCTTTCTATGTTGAAGAATTAGCATTTGGGATAAACATATGAAATTAAGAAATTAAAGTCTATAAATGATACTCAGCAACAATTTTCTTTGTGGTTCACATCTCAGGTCCGTGAACATGCTATTTTGCTTAATCTGGGATCGTTGCGAGCAATAGCTATGCAGGATTGTGTCCTCATATTCGATTATAACCGGTATCCATCTGACTTGAAAATGGTGCCATATTTATATCTCATACAAAATTTCGACATTGCAATTCCTTTTGCATGATGGTGATCATCTTCAGTCCAGGAGGACAAGCTTTTATTGAATCATTGTTGCCTCGATTGAACCCAAAAAACATGAATGGAGTGCCAGCAATGCCATTTGAACTCGAGGTTAGTAATTCTTGTTTATATATGTATTTTTACATCATATAAATAGAGTTGGCAGATGAATGCAAAATTAGCTTACAGACTGCGATACTTCTGTACACCTAACAATAAGATGTTTCCCAATTGGAATTTGGTCCTTAGGAAGCTTACCCCTAGAAGCAATGAAAAATTACCACTAACTGCCTTTTAGCATTCTTATATCATTCTGCGAGTCCCAATGGAAATTGCTGAGAAGATCAGATAATTATTGTTAGCAGATAAGTGAGATCAACTTCTGCTCACAAAGATTTTCAAATTAAAATCTGACCTTGAGAGGTCAATGAAGGTTCCTACCAATTCTGAAACTTACATCGAATATTTTCTATGATGGAGGCCAGACAACCAAATACTATTAAATAACCGTTGAGGGGAAGTCCAAATAGGTAGTGAGCCATTTCTCATTGTCTGCTATGAAGGCTAAACTTTTGCACTTCCAGATCATCTCTCGGCTAATAATCTGTCTCCGATTATTGTTTAGACCTGAGAAAGGCTATAAACGCTTGGTGATATTGAACGGATTAACAAACTCATCTTCAAGAGCAATGGAGAAAAGAATAGTACAAAACTTAATACAGAACCATTGGTCTAACCTTGGTTTTCCATAAGCAATAATGGTCTTTTAATCTTCTGGTGGCTTCTCCAGTTCCACGATAGCATTTACAAAATTTAAATCAACTATAACAAAGGCCTTTTATAGATCCAGTTTGACATATGCACCCTTCTTCCTTTTTCAAACATCACCGAATTCTGGACAAGAATCAATAATTTGTTTTCACCCGAAAAAGCCATTTGATGTTCTTTAATTGTGTATCAAATAATCCTTTAGATATTTTAATAAAAACTTAGTGGGTAAAACCATTAAGTTTGAACTTGTTAAGTCACTAGTTGACCCAAAAACTTAAGTTGATGTGTGAAGGTAAATGTAATATTATATCAAAACTTTAACACTCTCCTTTACTTGTGAGCTTGAAAATTTGTAGAAGACCCAATAAGTGGAAATCAATATTAACTGGGGAGGAAATAACATTGTAAGGTTTTGAACACAGGACCTCTTGCTCTGATACAACGTTAAATCACCAATTGATCCAAAAGCTTAAGCTAATGGGTGAAGGTAAATTTGATATTATATAATACTTTAACGGAACTCAATTACCTTTTTTGCATTACACTTCATTAGAATAAGGTGTACATCTCATTAATGCTCATTGCTCATGTGAGTAATGTCATTTTAGAAAAGTCCATGGAACAATTTCATAACTTATGCAGAAGAATGTTCGACCTTTTCTGAAGAACACTGCTGTTAAATGGTCTAGCCTAGGGGCCTTGCACATACAAACTTCGCATATCTTTCAAGATCTTGCCGTAGTAAAACTGATTCCCATGCCTTCCTTTAGTGATCAGAAAGAGGATTCAAATCAAGATTAG

mRNA sequence

TGGAAATTGATTTAATATAAAAAAAATACACCTATTTTTTTTTTCCCTTCTTCTTTCAAATCGAAGATCCCAAGAAGCTTAAAGCATACTTTTAATGTTCACAACCAGAGAGCTCGGATCGCTCTCCAATGGCGCTTTCTCTATCCCTACAACTTATCCGATTCCCTCCTCAAGCATTATCTTCATCCTCTCACCATCTTCACATCTCTGACCTTGGCTCCGTCTCGCGCTTCTTCCGATCTCCATGTCTTTCCTCTTTGACTCCGTCGCGGAGCTGCACGATGACGTTATCTTCCAGAACGAAATGTTTCTCCATGTCCACCGAGGAGGAGCAGTGGAGTGAACCGGAAGCGTTGGTGTCAGATGTGGACGGAGGGGGAGATCTTGAGGACGATATTGATATTGGTGTTCAGGAAGACAGCTCTGTGGCCACTGCTGCTTCTGCGCAGAGGATTTCTTCCTCGTCGCCGAGTGATTATCTGTCGCTTGCGATTCGAGAGCAGGTTTATGAGGTGTTAGAGGTTAAAGCTGATGGAACCGTATCTACCAGGAAGGTTAACAGACGGCAACTATTGAAGTCAAGTGTCTGCATTGACTTGTGTATACTTTCTTTCGGAAATGTCGATTACTACTGGATGTCTCACGGGCTTCGTCCACGTGATATCCGAAGTGTTGATCCATCTTTATTTTTGACAAACTCGATGCCTACATTACTGGTCCGTGAACATGCTATTTTGCTTAATCTGGGATCGTTGCGAGCAATAGCTATGCAGGATTGTGTCCTCATATTCGATTATAACCGTCCAGGAGGACAAGCTTTTATTGAATCATTGTTGCCTCGATTGAACCCAAAAAACATGAATGGAGTGCCAGCAATGCCATTTGAACTCGAGAAGAATGTTCGACCTTTTCTGAAGAACACTGCTGTTAAATGGTCTAGCCTAGGGGCCTTGCACATACAAACTTCGCATATCTTTCAAGATCTTGCCGTAGTAAAACTGATTCCCATGCCTTCCTTTAGTGATCAGAAAGAGGATTCAAATCAAGATTAG

Coding sequence (CDS)

ATGGCGCTTTCTCTATCCCTACAACTTATCCGATTCCCTCCTCAAGCATTATCTTCATCCTCTCACCATCTTCACATCTCTGACCTTGGCTCCGTCTCGCGCTTCTTCCGATCTCCATGTCTTTCCTCTTTGACTCCGTCGCGGAGCTGCACGATGACGTTATCTTCCAGAACGAAATGTTTCTCCATGTCCACCGAGGAGGAGCAGTGGAGTGAACCGGAAGCGTTGGTGTCAGATGTGGACGGAGGGGGAGATCTTGAGGACGATATTGATATTGGTGTTCAGGAAGACAGCTCTGTGGCCACTGCTGCTTCTGCGCAGAGGATTTCTTCCTCGTCGCCGAGTGATTATCTGTCGCTTGCGATTCGAGAGCAGGTTTATGAGGTGTTAGAGGTTAAAGCTGATGGAACCGTATCTACCAGGAAGGTTAACAGACGGCAACTATTGAAGTCAAGTGTCTGCATTGACTTGTGTATACTTTCTTTCGGAAATGTCGATTACTACTGGATGTCTCACGGGCTTCGTCCACGTGATATCCGAAGTGTTGATCCATCTTTATTTTTGACAAACTCGATGCCTACATTACTGGTCCGTGAACATGCTATTTTGCTTAATCTGGGATCGTTGCGAGCAATAGCTATGCAGGATTGTGTCCTCATATTCGATTATAACCGTCCAGGAGGACAAGCTTTTATTGAATCATTGTTGCCTCGATTGAACCCAAAAAACATGAATGGAGTGCCAGCAATGCCATTTGAACTCGAGAAGAATGTTCGACCTTTTCTGAAGAACACTGCTGTTAAATGGTCTAGCCTAGGGGCCTTGCACATACAAACTTCGCATATCTTTCAAGATCTTGCCGTAGTAAAACTGATTCCCATGCCTTCCTTTAGTGATCAGAAAGAGGATTCAAATCAAGATTAG

Protein sequence

MALSLSLQLIRFPPQALSSSSHHLHISDLGSVSRFFRSPCLSSLTPSRSCTMTLSSRTKCFSMSTEEEQWSEPEALVSDVDGGGDLEDDIDIGVQEDSSVATAASAQRISSSSPSDYLSLAIREQVYEVLEVKADGTVSTRKVNRRQLLKSSVCIDLCILSFGNVDYYWMSHGLRPRDIRSVDPSLFLTNSMPTLLVREHAILLNLGSLRAIAMQDCVLIFDYNRPGGQAFIESLLPRLNPKNMNGVPAMPFELEKNVRPFLKNTAVKWSSLGALHIQTSHIFQDLAVVKLIPMPSFSDQKEDSNQD
Homology
[Close]
BLAST of Lsi03G000690 vs. Swiss-Prot
Match: MRS2B_ARATH (Magnesium transporter MRS2-11, chloroplastic OS=Arabidopsis thaliana GN=MRS2-11 PE=2 SV=1)

HSP 1 Score: 199.5 bits (506), Expect = 5.3e-50
Identity = 132/239 (55.23%), Postives = 155/239 (64.85%), Query Frame = 1 

Query: 21  SHHLHISDLGSVSRFFRSPCLSSLTPSRSCTMTLSSRTKCFSMS--TEEEQWSEPEAL-V 80
           S  LH    GS   F       S TP     + + SR+KCF+ S  T E+   + E+L V
Sbjct: 24  SPSLHYLLPGSSPSFSLQLSALSRTPIYFEALKVLSRSKCFAKSPTTAEDFVGDYESLNV 83

Query: 81  SDVDGGGDLED-DIDIGVQEDSSVATAASAQRISSSSPSDYLSLAIREQVYEVLEVKADG 140
           SD D G D    D D G   D S    +S    SSSS SD  SL IRE VYEV+EVKA G
Sbjct: 84  SDDDDGSDSNSSDGDNGGGRDDSKKIDSS----SSSSSSDSTSLGIREPVYEVVEVKATG 143

Query: 141 TVSTRKVNRRQLLKSSVCIDLCILSFGNVDYYWMSHGLRPRDIRSVDPSLFLTNSMPTLL 200
            +STRK+NRRQLLKSS                    GLRPRDIRSVDPSLF+TNS+P+LL
Sbjct: 144 AISTRKINRRQLLKSS--------------------GLRPRDIRSVDPSLFMTNSVPSLL 203

Query: 201 VREHAILLNLGSLRAIAMQDCVLIFDYNRPGGQAFIESLLPRLNPKNMNGVPAMPFELE 256
           VREHAILLNLGSLRAIAM+D VLIFDYNR GG+AF+++L+PRLNP++MNG P+MPFELE
Sbjct: 204 VREHAILLNLGSLRAIAMRDRVLIFDYNRRGGRAFVDTLMPRLNPRSMNGGPSMPFELE 238

[Close]
BLAST of Lsi03G000690 vs. Swiss-Prot
Match: MRS2A_ORYSI (Magnesium transporter MRS2-A, chloroplastic OS=Oryza sativa subsp. indica GN=MRS2-A PE=3 SV=1)

HSP 1 Score: 173.3 bits (438), Expect = 4.0e-42
Identity = 100/184 (54.35%), Postives = 126/184 (68.48%), Query Frame = 1 

Query: 72  EPEALVSDVDGGGDLEDDIDIGVQEDSSVATAASAQRISSSSPSDYLSLAIREQVYEVLE 131
           E EA  + V+  G++E   +     D     AA+    S    +DY+SL IRE VYEV+E
Sbjct: 90  EEEAAAAAVEEEGEVEVRREEDKPGDDGSREAAARGSGSGRFSADYISLGIREPVYEVIE 149

Query: 132 VKADGTVSTRKVNRRQLLKSSVCIDLCILSFGNVDYYWMSHGLRPRDIRSVDPSLFLTNS 191
           VK++G +ST+K++RRQLLKSS                    GLR RD RSVDPSL+L NS
Sbjct: 150 VKSNGRMSTKKISRRQLLKSS--------------------GLRLRDTRSVDPSLWLMNS 209

Query: 192 MPTLLVREHAILLNLGSLRAIAMQDCVLIFDYNRPGGQAFIESLLPRLNPKNMNGVPAMP 251
           MP+LLVRE AIL+NLGSLRAIAM + VLIF+YN PGG+AF++SLLPRLNP+N+NG PAMP
Sbjct: 210 MPSLLVREQAILVNLGSLRAIAMHERVLIFNYNSPGGKAFLDSLLPRLNPRNINGGPAMP 253

Query: 252 FELE 256
           F+LE
Sbjct: 270 FQLE 253

[Close]
BLAST of Lsi03G000690 vs. Swiss-Prot
Match: MRS2A_ORYSJ (Magnesium transporter MRS2-A, chloroplastic OS=Oryza sativa subsp. japonica GN=MRS2-A PE=2 SV=1)

HSP 1 Score: 173.3 bits (438), Expect = 4.0e-42
Identity = 100/184 (54.35%), Postives = 126/184 (68.48%), Query Frame = 1 

Query: 72  EPEALVSDVDGGGDLEDDIDIGVQEDSSVATAASAQRISSSSPSDYLSLAIREQVYEVLE 131
           E EA  + V+  G++E   +     D     AA+    S    +DY+SL IRE VYEV+E
Sbjct: 90  EEEAAAAAVEEEGEVEVRREEDKPGDDGSREAAARGSGSGRFSADYISLGIREPVYEVIE 149

Query: 132 VKADGTVSTRKVNRRQLLKSSVCIDLCILSFGNVDYYWMSHGLRPRDIRSVDPSLFLTNS 191
           VK++G +ST+K++RRQLLKSS                    GLR RD RSVDPSL+L NS
Sbjct: 150 VKSNGRMSTKKISRRQLLKSS--------------------GLRLRDTRSVDPSLWLMNS 209

Query: 192 MPTLLVREHAILLNLGSLRAIAMQDCVLIFDYNRPGGQAFIESLLPRLNPKNMNGVPAMP 251
           MP+LLVRE AIL+NLGSLRAIAM + VLIF+YN PGG+AF++SLLPRLNP+N+NG PAMP
Sbjct: 210 MPSLLVREQAILVNLGSLRAIAMHERVLIFNYNSPGGKAFLDSLLPRLNPRNINGGPAMP 253

Query: 252 FELE 256
           F+LE
Sbjct: 270 FQLE 253

[Close]
BLAST of Lsi03G000690 vs. Swiss-Prot
Match: MRS2_NEUCR (Mitochondrial inner membrane magnesium transporter mrs2 OS=Neurospora crassa (strain ATCC 24698 / 74-OR23-1A / CBS 708.71 / DSM 1257 / FGSC 987) GN=mrs2 PE=3 SV=1)

HSP 1 Score: 58.5 bits (140), Expect = 1.4e-07
Identity = 33/86 (38.37%), Postives = 49/86 (56.98%), Query Frame = 1 

Query: 172 HGLRPRDIRSVDPSLFLTNSMPTLLVREHAILLNLGSLRAIAMQDCVLIFD-YNRPGG-- 231
           +GL PRD+R +D S     ++P +L+R  AILLNL  L+ +   DCVL+FD Y       
Sbjct: 207 YGLLPRDLRKIDSS-----NLPHILIRPSAILLNLLHLKVLIKHDCVLLFDVYGSKSSYP 266

Query: 232 -QAFIESLLPRLNPKNMNGVPAMPFE 254
             AF+  L  +L  K  +G  ++P+E
Sbjct: 267 QSAFMYDLQGKLQQKQSSGANSLPYE 287

[Close]
BLAST of Lsi03G000690 vs. Swiss-Prot
Match: MRS2_ASPFU (Mitochondrial inner membrane magnesium transporter mrs2 OS=Neosartorya fumigata (strain ATCC MYA-4609 / Af293 / CBS 101355 / FGSC A1100) GN=mrs2 PE=3 SV=1)

HSP 1 Score: 53.5 bits (127), Expect = 4.7e-06
Identity = 33/86 (38.37%), Postives = 46/86 (53.49%), Query Frame = 1 

Query: 172 HGLRPRDIRSVDPSLFLTNSMPTLLVREHAILLNLGSLRAIAMQDCVLIFD-YNRPGG-- 231
           +GL PRD+R +D S     ++P +LVR  AIL+NL  LR +   D VL+FD Y       
Sbjct: 202 YGLLPRDLRKIDSS-----TLPHILVRPSAILINLLHLRVLIKHDRVLVFDAYGSTDSYM 261

Query: 232 -QAFIESLLPRLNPKNMNGVPAMPFE 254
              F+  L  +L  K   G  A+P+E
Sbjct: 262 QSLFVYDLEGKLQQKQTGGFGALPYE 282

[Close]
BLAST of Lsi03G000690 vs. TrEMBL
Match: A0A0A0L1G8_CUCSA (Uncharacterized protein OS=Cucumis sativus GN=Csa_4G209330 PE=4 SV=1)

HSP 1 Score: 224.2 bits (570), Expect = 2.2e-55
Identity = 118/143 (82.52%), Postives = 120/143 (83.92%), Query Frame = 1 

Query: 129 VLEVKADGTVSTRKVNRRQLLKSSVCIDLCILSFGNVDYYWMSHGLRPRDIRSVDPSLFL 188
           VLEVKADGTVSTRKVNRRQLLKSS                    GLRPRD+RSVDPSLFL
Sbjct: 2   VLEVKADGTVSTRKVNRRQLLKSS--------------------GLRPRDVRSVDPSLFL 61

Query: 189 TNSMPTLLVREHAILLNLGSLRAIAMQDCVLIFDYNRPGGQAFIESLLPRLNPKNMNGVP 248
           TNSMPTLLVREHAILLNLGSLRAIAMQDCVLIFD+NRPGGQAFIESLLPRLNPKNMNGVP
Sbjct: 62  TNSMPTLLVREHAILLNLGSLRAIAMQDCVLIFDHNRPGGQAFIESLLPRLNPKNMNGVP 121

Query: 249 AMPFELEKNVRPFLKNTAVKWSS 272
           AMPFELEKNV PFLKN AVK SS
Sbjct: 122 AMPFELEKNVHPFLKNAAVKQSS 124

[Close]
BLAST of Lsi03G000690 vs. TrEMBL
Match: A5C9Y2_VITVI (Putative uncharacterized protein OS=Vitis vinifera GN=VITISV_026281 PE=4 SV=1)

HSP 1 Score: 219.5 bits (558), Expect = 5.5e-54
Identity = 144/262 (54.96%), Postives = 172/262 (65.65%), Query Frame = 1 

Query: 1   MALSLSLQLIRFPPQALSSSSHHLHISDLGSVSRFFRS----PCLSSLTPSRSCTMTLS- 60
           +A S S  L++FP   +SS +H L +    + + FFRS    P   SL  + S    +S 
Sbjct: 3   LAPSSSPHLLQFP---VSSPAHRLLLFSDLTCATFFRSSWPPPFPISLKKANSFVFPISV 62

Query: 61  SRTKCFSMSTEEEQWSEPEALVSDVDGGGDLEDDIDIGVQEDSSVATAASAQRISSSSPS 120
            R KCFS STEE+  S+ E       GG D            +SVAT   +QRI++SS +
Sbjct: 63  RREKCFSKSTEEDLLSDSETQT----GGND---------DRPASVATTGGSQRIAASS-T 122

Query: 121 DYLSLAIREQVYEVLEVKADGTVSTRKVNRRQLLKSSVCIDLCILSFGNVDYYWMSHGLR 180
           D LSL IRE VYEV+EVK+DG VSTRK+NRRQLLKSS                    GLR
Sbjct: 123 DSLSLGIREPVYEVVEVKSDGKVSTRKINRRQLLKSS--------------------GLR 182

Query: 181 PRDIRSVDPSLFLTNSMPTLLVREHAILLNLGSLRAIAMQDCVLIFDYNRPGGQAFIESL 240
           PRDIRSVDPSL+LTNSMP+L+VREHAILLNLGSLRAIAMQ+ V IFDYNR GG+AF++SL
Sbjct: 183 PRDIRSVDPSLWLTNSMPSLVVREHAILLNLGSLRAIAMQESVFIFDYNRKGGKAFMDSL 227

Query: 241 LPRLNPKNMNGVPAMPFELEKN 258
           LPRLNPKNMNG P+MPFELE N
Sbjct: 243 LPRLNPKNMNGGPSMPFELELN 227

[Close]
BLAST of Lsi03G000690 vs. TrEMBL
Match: F6GW66_VITVI (Putative uncharacterized protein OS=Vitis vinifera GN=VIT_06s0061g01060 PE=4 SV=1)

HSP 1 Score: 218.0 bits (554), Expect = 1.6e-53
Identity = 143/260 (55.00%), Postives = 171/260 (65.77%), Query Frame = 1 

Query: 1   MALSLSLQLIRFPPQALSSSSHHLHISDLGSVSRFFRS----PCLSSLTPSRSCTMTLS- 60
           +A S S  L++FP   +SS +H L +    + + FFRS    P   SL  + S    +S 
Sbjct: 3   LAPSSSPHLLQFP---VSSPAHRLLLFSDLTCATFFRSSWPPPFPISLKKANSFVFPISV 62

Query: 61  SRTKCFSMSTEEEQWSEPEALVSDVDGGGDLEDDIDIGVQEDSSVATAASAQRISSSSPS 120
            R KCFS STEE+  S+ E       GG D            +SVAT   +QRI++SS +
Sbjct: 63  RREKCFSKSTEEDLLSDSETQT----GGND---------DRPASVATTGGSQRIAASS-T 122

Query: 121 DYLSLAIREQVYEVLEVKADGTVSTRKVNRRQLLKSSVCIDLCILSFGNVDYYWMSHGLR 180
           D LSL IRE VYEV+EVK+DG VSTRK+NRRQLLKSS                    GLR
Sbjct: 123 DSLSLGIREPVYEVVEVKSDGKVSTRKINRRQLLKSS--------------------GLR 182

Query: 181 PRDIRSVDPSLFLTNSMPTLLVREHAILLNLGSLRAIAMQDCVLIFDYNRPGGQAFIESL 240
           PRDIRSVDPSL+LTNSMP+L+VREHAILLNLGSLRAIAMQ+ V IFDYNR GG+AF++SL
Sbjct: 183 PRDIRSVDPSLWLTNSMPSLVVREHAILLNLGSLRAIAMQESVFIFDYNRKGGKAFMDSL 225

Query: 241 LPRLNPKNMNGVPAMPFELE 256
           LPRLNPKNMNG P+MPFELE
Sbjct: 243 LPRLNPKNMNGGPSMPFELE 225

[Close]
BLAST of Lsi03G000690 vs. TrEMBL
Match: K7LVN0_SOYBN (Uncharacterized protein OS=Glycine max GN=GLYMA_12G180000 PE=4 SV=1)

HSP 1 Score: 216.1 bits (549), Expect = 6.0e-53
Identity = 141/263 (53.61%), Postives = 168/263 (63.88%), Query Frame = 1 

Query: 1   MALSLSLQLIRFPPQALSSSSHHLHISDLGSVSRFFRSPCLSSLTPSRSCTM------TL 60
           MALS    +++F  Q L   ++ +  SDL   SR     C  S T  R C +      T+
Sbjct: 1   MALSWP-HVLQFRSQPLLGHANCVFFSDLPE-SRSLELSCSDSYTLLRRCKISGHSPATV 60

Query: 61  SSRTKCFSMSTEEEQWSEPEALVSDVDGGGDLEDDIDIGVQEDSSVATAA--SAQRISSS 120
               KC S STEE+QWS+ E  VSD D   D  +D     QE +S  T     +QRI+++
Sbjct: 61  KPAVKCLSRSTEEKQWSDAETAVSDSDEVADEPND-----QERTSAPTNGRFESQRIATT 120

Query: 121 SPSDYLSLAIREQVYEVLEVKADGTVSTRKVNRRQLLKSSVCIDLCILSFGNVDYYWMSH 180
           S  D LSL IRE VYEV+EV+++G VSTRK+NRRQLLKSS                    
Sbjct: 121 SSGDSLSLGIREPVYEVVEVRSNGKVSTRKINRRQLLKSS-------------------- 180

Query: 181 GLRPRDIRSVDPSLFLTNSMPTLLVREHAILLNLGSLRAIAMQDCVLIFDYNRPGGQAFI 240
           GLRPRDIRSVDPSLF+TNSMP LLVRE+AILLNLGSLRAIAMQDCVLIFD N  GG+AF+
Sbjct: 181 GLRPRDIRSVDPSLFMTNSMPALLVREYAILLNLGSLRAIAMQDCVLIFDNNGIGGKAFL 236

Query: 241 ESLLPRLNPKNMNGVPAMPFELE 256
           E+LLPRLNPK  NG P+MPFELE
Sbjct: 241 ETLLPRLNPKINNGGPSMPFELE 236

[Close]
BLAST of Lsi03G000690 vs. TrEMBL
Match: A0A0R0H6R7_SOYBN (Uncharacterized protein OS=Glycine max GN=GLYMA_12G180000 PE=4 SV=1)

HSP 1 Score: 216.1 bits (549), Expect = 6.0e-53
Identity = 141/263 (53.61%), Postives = 168/263 (63.88%), Query Frame = 1 

Query: 1   MALSLSLQLIRFPPQALSSSSHHLHISDLGSVSRFFRSPCLSSLTPSRSCTM------TL 60
           MALS    +++F  Q L   ++ +  SDL   SR     C  S T  R C +      T+
Sbjct: 1   MALSWP-HVLQFRSQPLLGHANCVFFSDLPE-SRSLELSCSDSYTLLRRCKISGHSPATV 60

Query: 61  SSRTKCFSMSTEEEQWSEPEALVSDVDGGGDLEDDIDIGVQEDSSVATAA--SAQRISSS 120
               KC S STEE+QWS+ E  VSD D   D  +D     QE +S  T     +QRI+++
Sbjct: 61  KPAVKCLSRSTEEKQWSDAETAVSDSDEVADEPND-----QERTSAPTNGRFESQRIATT 120

Query: 121 SPSDYLSLAIREQVYEVLEVKADGTVSTRKVNRRQLLKSSVCIDLCILSFGNVDYYWMSH 180
           S  D LSL IRE VYEV+EV+++G VSTRK+NRRQLLKSS                    
Sbjct: 121 SSGDSLSLGIREPVYEVVEVRSNGKVSTRKINRRQLLKSS-------------------- 180

Query: 181 GLRPRDIRSVDPSLFLTNSMPTLLVREHAILLNLGSLRAIAMQDCVLIFDYNRPGGQAFI 240
           GLRPRDIRSVDPSLF+TNSMP LLVRE+AILLNLGSLRAIAMQDCVLIFD N  GG+AF+
Sbjct: 181 GLRPRDIRSVDPSLFMTNSMPALLVREYAILLNLGSLRAIAMQDCVLIFDNNGIGGKAFL 236

Query: 241 ESLLPRLNPKNMNGVPAMPFELE 256
           E+LLPRLNPK  NG P+MPFELE
Sbjct: 241 ETLLPRLNPKINNGGPSMPFELE 236

[Close]
BLAST of Lsi03G000690 vs. TAIR10
Match: AT5G22830.1 (AT5G22830.1 magnesium (Mg) transporter 10)

HSP 1 Score: 199.5 bits (506), Expect = 3.0e-51
Identity = 132/239 (55.23%), Postives = 155/239 (64.85%), Query Frame = 1 

Query: 21  SHHLHISDLGSVSRFFRSPCLSSLTPSRSCTMTLSSRTKCFSMS--TEEEQWSEPEAL-V 80
           S  LH    GS   F       S TP     + + SR+KCF+ S  T E+   + E+L V
Sbjct: 24  SPSLHYLLPGSSPSFSLQLSALSRTPIYFEALKVLSRSKCFAKSPTTAEDFVGDYESLNV 83

Query: 81  SDVDGGGDLED-DIDIGVQEDSSVATAASAQRISSSSPSDYLSLAIREQVYEVLEVKADG 140
           SD D G D    D D G   D S    +S    SSSS SD  SL IRE VYEV+EVKA G
Sbjct: 84  SDDDDGSDSNSSDGDNGGGRDDSKKIDSS----SSSSSSDSTSLGIREPVYEVVEVKATG 143

Query: 141 TVSTRKVNRRQLLKSSVCIDLCILSFGNVDYYWMSHGLRPRDIRSVDPSLFLTNSMPTLL 200
            +STRK+NRRQLLKSS                    GLRPRDIRSVDPSLF+TNS+P+LL
Sbjct: 144 AISTRKINRRQLLKSS--------------------GLRPRDIRSVDPSLFMTNSVPSLL 203

Query: 201 VREHAILLNLGSLRAIAMQDCVLIFDYNRPGGQAFIESLLPRLNPKNMNGVPAMPFELE 256
           VREHAILLNLGSLRAIAM+D VLIFDYNR GG+AF+++L+PRLNP++MNG P+MPFELE
Sbjct: 204 VREHAILLNLGSLRAIAMRDRVLIFDYNRRGGRAFVDTLMPRLNPRSMNGGPSMPFELE 238

[Close]
BLAST of Lsi03G000690 vs. NCBI nr
Match: gi|659071865|ref|XP_008462287.1| (PREDICTED: LOW QUALITY PROTEIN: magnesium transporter MRS2-11, chloroplastic [Cucumis melo])

HSP 1 Score: 393.7 bits (1010), Expect = 3.0e-106
Identity = 218/258 (84.50%), Postives = 223/258 (86.43%), Query Frame = 1 

Query: 1   MALSLSLQLIRFPPQALSSSSHHLHISDLGSVSRFFRSPCLSSLTPSRSCTMTLSSRTKC 60
           MALSLSLQLIRFPP+ LSSSSHHL ISDL SVSRFFRSPCLSSL PSR C + LSSRTKC
Sbjct: 1   MALSLSLQLIRFPPRPLSSSSHHLLISDLTSVSRFFRSPCLSSLAPSRDCALKLSSRTKC 60

Query: 61  FSMSTEEEQWSEPEALVSDVDGGGDLEDDIDIGVQEDSSVATAASAQRI---SSSSPSDY 120
           FSMSTEEEQWSE EA VSDVD GGDLEDDIDI V+EDSSVATAASAQRI   SSSSPSDY
Sbjct: 61  FSMSTEEEQWSESEAFVSDVDEGGDLEDDIDISVREDSSVATAASAQRISSSSSSSPSDY 120

Query: 121 LSLAIREQVYEVLEVKADGTVSTRKVNRRQLLKSSVCIDLCILSFGNVDYYWMSHGLRPR 180
           LSLAIREQVYEVLEVKA+GTVSTRKVNRRQLLKSS                    GLRPR
Sbjct: 121 LSLAIREQVYEVLEVKANGTVSTRKVNRRQLLKSS--------------------GLRPR 180

Query: 181 DIRSVDPSLFLTNSMPTLLVREHAILLNLGSLRAIAMQDCVLIFDYNRPGGQAFIESLLP 240
           D+RSVDPSLFLTNSMPTLLVREHAILLNLGSLRAIAMQDCVLIFDYNRPGGQAFIESLLP
Sbjct: 181 DVRSVDPSLFLTNSMPTLLVREHAILLNLGSLRAIAMQDCVLIFDYNRPGGQAFIESLLP 238

Query: 241 RLNPKNMNGVPAMPFELE 256
           RLNPK MNGVPAMPFELE
Sbjct: 241 RLNPKXMNGVPAMPFELE 238

[Close]
BLAST of Lsi03G000690 vs. NCBI nr
Match: gi|700198813|gb|KGN53971.1| (hypothetical protein Csa_4G209330 [Cucumis sativus])

HSP 1 Score: 224.2 bits (570), Expect = 3.2e-55
Identity = 118/143 (82.52%), Postives = 120/143 (83.92%), Query Frame = 1 

Query: 129 VLEVKADGTVSTRKVNRRQLLKSSVCIDLCILSFGNVDYYWMSHGLRPRDIRSVDPSLFL 188
           VLEVKADGTVSTRKVNRRQLLKSS                    GLRPRD+RSVDPSLFL
Sbjct: 2   VLEVKADGTVSTRKVNRRQLLKSS--------------------GLRPRDVRSVDPSLFL 61

Query: 189 TNSMPTLLVREHAILLNLGSLRAIAMQDCVLIFDYNRPGGQAFIESLLPRLNPKNMNGVP 248
           TNSMPTLLVREHAILLNLGSLRAIAMQDCVLIFD+NRPGGQAFIESLLPRLNPKNMNGVP
Sbjct: 62  TNSMPTLLVREHAILLNLGSLRAIAMQDCVLIFDHNRPGGQAFIESLLPRLNPKNMNGVP 121

Query: 249 AMPFELEKNVRPFLKNTAVKWSS 272
           AMPFELEKNV PFLKN AVK SS
Sbjct: 122 AMPFELEKNVHPFLKNAAVKQSS 124

[Close]
BLAST of Lsi03G000690 vs. NCBI nr
Match: gi|1012221590|ref|XP_015935900.1| (PREDICTED: magnesium transporter MRS2-11, chloroplastic isoform X1 [Arachis duranensis])

HSP 1 Score: 223.8 bits (569), Expect = 4.2e-55
Identity = 140/265 (52.83%), Postives = 169/265 (63.77%), Query Frame = 1 

Query: 1   MALSLSLQLIRFPPQALSSSSHHLHISDLGSVSRFFRSPCLSS---------LTPSRSCT 60
           MAL   L L     Q L + ++    SDL    R F +PCL S         L P+    
Sbjct: 1   MALRWPLLLRFRSQQPLLAPTNFAAFSDLAD-PRAFDAPCLPSVNLLHRSNVLAPALKLK 60

Query: 61  MTLSSRTKCFSMSTEEEQWSEPEALVSDVDGGGDLED-DIDIGVQEDSSVATAASAQRIS 120
           +      KC + STEE+QWS+ E +V+D D     +D +  +  Q  +S      + R++
Sbjct: 61  VLAPYVVKCLARSTEEKQWSDAETVVTDSDEAASEDDGNGQLRSQTSASETDRLESPRVA 120

Query: 121 SSSPSDYLSLAIREQVYEVLEVKADGTVSTRKVNRRQLLKSSVCIDLCILSFGNVDYYWM 180
           +S   D LSL IRE VYEV+EV++DGTVSTRK+NRRQLLKSS                  
Sbjct: 121 TSYSGDSLSLGIREPVYEVVEVRSDGTVSTRKINRRQLLKSS------------------ 180

Query: 181 SHGLRPRDIRSVDPSLFLTNSMPTLLVREHAILLNLGSLRAIAMQDCVLIFDYNRPGGQA 240
             GLRPRDIRSVDPSLFLTNS+P+LLVRE+AILLNLGSLRAIAM+DCVLIFDYNR GGQA
Sbjct: 181 --GLRPRDIRSVDPSLFLTNSVPSLLVREYAILLNLGSLRAIAMKDCVLIFDYNRKGGQA 240

Query: 241 FIESLLPRLNPKNMNGVPAMPFELE 256
           F+ESLLPRLNPKN NG P+MPFELE
Sbjct: 241 FLESLLPRLNPKNSNGGPSMPFELE 244

[Close]
BLAST of Lsi03G000690 vs. NCBI nr
Match: gi|1012221594|ref|XP_015935901.1| (PREDICTED: magnesium transporter MRS2-11, chloroplastic isoform X2 [Arachis duranensis])

HSP 1 Score: 223.8 bits (569), Expect = 4.2e-55
Identity = 140/265 (52.83%), Postives = 169/265 (63.77%), Query Frame = 1 

Query: 1   MALSLSLQLIRFPPQALSSSSHHLHISDLGSVSRFFRSPCLSS---------LTPSRSCT 60
           MAL   L L     Q L + ++    SDL    R F +PCL S         L P+    
Sbjct: 1   MALRWPLLLRFRSQQPLLAPTNFAAFSDLAD-PRAFDAPCLPSVNLLHRSNVLAPALKLK 60

Query: 61  MTLSSRTKCFSMSTEEEQWSEPEALVSDVDGGGDLED-DIDIGVQEDSSVATAASAQRIS 120
           +      KC + STEE+QWS+ E +V+D D     +D +  +  Q  +S      + R++
Sbjct: 61  VLAPYVVKCLARSTEEKQWSDAETVVTDSDEAASEDDGNGQLRSQTSASETDRLESPRVA 120

Query: 121 SSSPSDYLSLAIREQVYEVLEVKADGTVSTRKVNRRQLLKSSVCIDLCILSFGNVDYYWM 180
           +S   D LSL IRE VYEV+EV++DGTVSTRK+NRRQLLKSS                  
Sbjct: 121 TSYSGDSLSLGIREPVYEVVEVRSDGTVSTRKINRRQLLKSS------------------ 180

Query: 181 SHGLRPRDIRSVDPSLFLTNSMPTLLVREHAILLNLGSLRAIAMQDCVLIFDYNRPGGQA 240
             GLRPRDIRSVDPSLFLTNS+P+LLVRE+AILLNLGSLRAIAM+DCVLIFDYNR GGQA
Sbjct: 181 --GLRPRDIRSVDPSLFLTNSVPSLLVREYAILLNLGSLRAIAMKDCVLIFDYNRKGGQA 240

Query: 241 FIESLLPRLNPKNMNGVPAMPFELE 256
           F+ESLLPRLNPKN NG P+MPFELE
Sbjct: 241 FLESLLPRLNPKNSNGGPSMPFELE 244

[Close]
BLAST of Lsi03G000690 vs. NCBI nr
Match: gi|778659302|ref|XP_011654160.1| (PREDICTED: magnesium transporter MRS2-11, chloroplastic, partial [Cucumis sativus])

HSP 1 Score: 223.4 bits (568), Expect = 5.4e-55
Identity = 120/145 (82.76%), Postives = 122/145 (84.14%), Query Frame = 1 

Query: 111 SSSPSDYLSLAIREQVYEVLEVKADGTVSTRKVNRRQLLKSSVCIDLCILSFGNVDYYWM 170
           SS  SDYLSLAI EQVYEVLEVKADGTVSTRKVNRRQLLKSS                  
Sbjct: 13  SSFTSDYLSLAIPEQVYEVLEVKADGTVSTRKVNRRQLLKSS------------------ 72

Query: 171 SHGLRPRDIRSVDPSLFLTNSMPTLLVREHAILLNLGSLRAIAMQDCVLIFDYNRPGGQA 230
             GLRPRD+RSVDPSLFLTNSMPTLLVREHAILLNLGSLRAIAMQDCVLIFD+NRPGGQA
Sbjct: 73  --GLRPRDVRSVDPSLFLTNSMPTLLVREHAILLNLGSLRAIAMQDCVLIFDHNRPGGQA 132

Query: 231 FIESLLPRLNPKNMNGVPAMPFELE 256
           FIESLLPRLNPKNMNGVPAMPFELE
Sbjct: 133 FIESLLPRLNPKNMNGVPAMPFELE 137

The following BLAST results are available for this feature:
BLAST of Lsi03G000690 vs. Swiss-Prot
Match NameE-valueIdentityDescription
MRS2B_ARATH5.3e-5055.23Magnesium transporter MRS2-11, chloroplastic OS=Arabidopsis thaliana GN=MRS2-11 ... [more]
MRS2A_ORYSI4.0e-4254.35Magnesium transporter MRS2-A, chloroplastic OS=Oryza sativa subsp. indica GN=MRS... [more]
MRS2A_ORYSJ4.0e-4254.35Magnesium transporter MRS2-A, chloroplastic OS=Oryza sativa subsp. japonica GN=M... [more]
MRS2_NEUCR1.4e-0738.37Mitochondrial inner membrane magnesium transporter mrs2 OS=Neurospora crassa (st... [more]
MRS2_ASPFU4.7e-0638.37Mitochondrial inner membrane magnesium transporter mrs2 OS=Neosartorya fumigata ... [more]
BLAST of Lsi03G000690 vs. TrEMBL
Match NameE-valueIdentityDescription
A0A0A0L1G8_CUCSA2.2e-5582.52Uncharacterized protein OS=Cucumis sativus GN=Csa_4G209330 PE=4 SV=1[more]
A5C9Y2_VITVI5.5e-5454.96Putative uncharacterized protein OS=Vitis vinifera GN=VITISV_026281 PE=4 SV=1[more]
F6GW66_VITVI1.6e-5355.00Putative uncharacterized protein OS=Vitis vinifera GN=VIT_06s0061g01060 PE=4 SV=... [more]
K7LVN0_SOYBN6.0e-5353.61Uncharacterized protein OS=Glycine max GN=GLYMA_12G180000 PE=4 SV=1[more]
A0A0R0H6R7_SOYBN6.0e-5353.61Uncharacterized protein OS=Glycine max GN=GLYMA_12G180000 PE=4 SV=1[more]
BLAST of Lsi03G000690 vs. TAIR10
Match NameE-valueIdentityDescription
AT5G22830.13.0e-5155.23 magnesium (Mg) transporter 10[more]
BLAST of Lsi03G000690 vs. NCBI nr
Match NameE-valueIdentityDescription
gi|659071865|ref|XP_008462287.1|3.0e-10684.50PREDICTED: LOW QUALITY PROTEIN: magnesium transporter MRS2-11, chloroplastic [Cu... [more]
gi|700198813|gb|KGN53971.1|3.2e-5582.52hypothetical protein Csa_4G209330 [Cucumis sativus][more]
gi|1012221590|ref|XP_015935900.1|4.2e-5552.83PREDICTED: magnesium transporter MRS2-11, chloroplastic isoform X1 [Arachis dura... [more]
gi|1012221594|ref|XP_015935901.1|4.2e-5552.83PREDICTED: magnesium transporter MRS2-11, chloroplastic isoform X2 [Arachis dura... [more]
gi|778659302|ref|XP_011654160.1|5.4e-5582.76PREDICTED: magnesium transporter MRS2-11, chloroplastic, partial [Cucumis sativu... [more]
Annotated Terms
The following terms have been associated with this gene:
Vocabulary: INTERPRO
TermDefinition
GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0015693 magnesium ion transport
biological_process GO:0044763 single-organism cellular process
biological_process GO:0055085 transmembrane transport
biological_process GO:0030003 cellular cation homeostasis
biological_process GO:0030001 metal ion transport
biological_process GO:0008150 biological_process
cellular_component GO:0005575 cellular_component
cellular_component GO:0009507 chloroplast
cellular_component GO:0016020 membrane
cellular_component GO:0009941 chloroplast envelope
cellular_component GO:0016021 integral component of membrane
molecular_function GO:0015095 magnesium ion transmembrane transporter activity
molecular_function GO:0046873 metal ion transmembrane transporter activity
molecular_function GO:0003674 molecular_function
Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Lsi03G000690.1Lsi03G000690.1mRNA


InterPro
Analysis Name: InterPro Annotations of Lagenaria siceraria
Date Performed: 2017-09-18
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
NoneNo IPR availablePANTHERPTHR13890RNA SPLICING PROTEIN MRS2, MITOCHONDRIALcoord: 171..255
score: 1.0E-46coord: 41..150
score: 1.0
NoneNo IPR availablePANTHERPTHR13890:SF0MAGNESIUM TRANSPORTER MRS2 HOMOLOG, MITOCHONDRIALcoord: 171..255
score: 1.0E-46coord: 41..150
score: 1.0