Lsi02G028920 (gene) Bottle gourd (USVL1VR-Ls)

BLAST of Lsi02G028920 vs. Swiss-Prot
Match: SBT14_ARATH (Subtilisin-like protease SBT1.4 OS=Arabidopsis thaliana GN=SBT1.4 PE=2 SV=1)

HSP 1 Score: 988.0 bits (2553), Expect = 5.7e-287
Identity = 500/771 (64.85%), Postives = 599/771 (77.69%), Query Frame = 1

Query: 8   SSLFFFF--ILCFVPVIFSRSPDNQETFIVHVSKSEKPSLFSSHNHWHSSILESLSPSPH 67
           SS+FF F  +LCF     S S D  E++IVHV +S KPSLFSSHN+WH S+L SL  SP 
Sbjct: 7   SSIFFVFPLLLCFFSPS-SSSSDGLESYIVHVQRSHKPSLFSSHNNWHVSLLRSLPSSPQ 66

Query: 68  PTKLLYSYERAVNGFSASITAAQAAELRHVPGVISVIPDRIRQLHTTRTPHFLGLADNLG 127
           P  LLYSY RAV+GFSA ++  Q A LR  P VISVIPD+ R++HTT TP FLG + N G
Sbjct: 67  PATLLYSYSRAVHGFSARLSPIQTAALRRHPSVISVIPDQAREIHTTHTPAFLGFSQNSG 126

Query: 128 LWADTNYAEDVIIGVLDTGIWPERPSFSDEGLSPVPASWKGTCETGEGFSASACNRKIIG 187
           LW+++NY EDVI+GVLDTGIWPE PSFSD GL P+P++WKG CE G  F AS+CNRK+IG
Sbjct: 127 LWSNSNYGEDVIVGVLDTGIWPEHPSFSDSGLGPIPSTWKGECEIGPDFPASSCNRKLIG 186

Query: 188 ARAYFYGY--ESNLGRSLRSSDFKSARDTDGHGTHTASTAAGSFVKNASFFQYARGEARG 247
           ARA++ GY  + N  +   + + +S RDT+GHGTHTASTAAGS V NAS +QYARG A G
Sbjct: 187 ARAFYRGYLTQRNGTKKHAAKESRSPRDTEGHGTHTASTAAGSVVANASLYQYARGTATG 246

Query: 248 MASRARIAAYKICWEYGCFDSDILAAMDQAIADGVHIISLSVGSSGRAPAYYRDSIAIGA 307
           MAS+ARIAAYKICW  GC+DSDILAAMDQA+ADGVH+ISLSVG+SG AP Y+ DSIAIGA
Sbjct: 247 MASKARIAAYKICWTGGCYDSDILAAMDQAVADGVHVISLSVGASGSAPEYHTDSIAIGA 306

Query: 308 FGAMQHGVVVSCSAGNSGPGSYTAVNIAPWILTVGASTIDREFLADVILGDGRVFSGVSL 367
           FGA +HG+VVSCSAGNSGP   TA NIAPWILTVGAST+DREF A+ I GDG+VF+G SL
Sbjct: 307 FGATRHGIVVSCSAGNSGPNPETATNIAPWILTVGASTVDREFAANAITGDGKVFTGTSL 366

Query: 368 YSGEPLGDSKLPLVYGGDCGSRYCYSGSLDSSKVAGKIVVCDRGGNARVAKGSAVKSAGG 427
           Y+GE L DS+L LVY GDCGSR CY G L+SS V GKIV+CDRGGNARV KGSAVK AGG
Sbjct: 367 YAGESLPDSQLSLVYSGDCGSRLCYPGKLNSSLVEGKIVLCDRGGNARVEKGSAVKLAGG 426

Query: 428 LGMVLANTEENGEELLADSHLIPGTMVGEIAGNKLRNYTHSDPNPTATIVFRGTVIGNSP 487
            GM+LANT E+GEEL ADSHL+P TMVG  AG+++R+Y  +  +PTA I F GT+IG SP
Sbjct: 427 AGMILANTAESGEELTADSHLVPATMVGAKAGDQIRDYIKTSDSPTAKISFLGTLIGPSP 486

Query: 488 SAPRVAAFSSRGPNYRTAEILKPDVIAPGVNILAGWSGYSSPTSLSIDPRRVEFNIISGT 547
            +PRVAAFSSRGPN+ T  ILKPDVIAPGVNILAGW+G   PT L IDPRRV+FNIISGT
Sbjct: 487 PSPRVAAFSSRGPNHLTPVILKPDVIAPGVNILAGWTGMVGPTDLDIDPRRVQFNIISGT 546

Query: 548 SMSCPHVSGVAALLRKAFPTWSPAAIKSALITTSYSLDSSGSPIKDLSTSKESNPFVHGA 607
           SMSCPHVSG+AALLRKA P WSPAAIKSAL+TT+Y +++SG PI+DL+T K SN F+HGA
Sbjct: 547 SMSCPHVSGLAALLRKAHPDWSPAAIKSALVTTAYDVENSGEPIEDLATGKSSNSFIHGA 606

Query: 608 GHIDPNKALNPGLIYDLTPQDYVSFLCSIGYDSRQIAVFVKDSSYSQLCE-HKLSNPGNL 667
           GH+DPNKALNPGL+YD+  ++YV+FLC++GY+   I VF++D +    CE  KL   G+L
Sbjct: 607 GHVDPNKALNPGLVYDIEVKEYVAFLCAVGYEFPGILVFLQDPTLYDACETSKLRTAGDL 666

Query: 668 NYPSFAVVF-DDEEVVKYTRTVTNVGDETDAVYEVKVESPQGVEISVVPNKLEFNEKKTT 727
           NYPSF+VVF    EVVKY R V NVG   DAVYEV V+SP  VEI V P+KL F+++K+ 
Sbjct: 667 NYPSFSVVFASTGEVVKYKRVVKNVGSNVDAVYEVGVKSPANVEIDVSPSKLAFSKEKSV 726

Query: 728 QSYEITFTKI-----SGFKELASFGSIQWSDGSHNVRSPIAVSFNTGSIAS 768
             YE+TF  +      G      FGSI+W+DG H V+SP+AV +  GS+ S
Sbjct: 727 LEYEVTFKSVVLGGGVGSVPGHEFGSIEWTDGEHVVKSPVAVQWGQGSVQS 776

BLAST of Lsi02G028920 vs. Swiss-Prot
Match: SBT17_ARATH (Subtilisin-like protease SBT1.7 OS=Arabidopsis thaliana GN=SBT1.7 PE=1 SV=1)

HSP 1 Score: 792.7 bits (2046), Expect = 3.5e-228
Identity = 426/764 (55.76%), Postives = 532/764 (69.63%), Query Frame = 1

Query: 8   SSLFFFFILCFVPVIFSRSPDNQETFIVHVSKSEKPSLFSSHNHWHSSILESLSPSPHPT 67
           SS  FF +LC      S S  +Q T+IVH++KS+ PS F  H++W+ S L S+S S    
Sbjct: 7   SSTAFFLLLCLGFCHVSSSSSDQGTYIVHMAKSQMPSSFDLHSNWYDSSLRSISDS---A 66

Query: 68  KLLYSYERAVNGFSASITAAQAAELRHVPGVISVIPDRIRQLHTTRTPHFLGLADNLG-L 127
           +LLY+YE A++GFS  +T  +A  L   PGVISV+P+   +LHTTRTP FLGL ++   L
Sbjct: 67  ELLYTYENAIHGFSTRLTQEEADSLMTQPGVISVLPEHRYELHTTRTPLFLGLDEHTADL 126

Query: 128 WADTNYAEDVIIGVLDTGIWPERPSFSDEGLSPVPASWKGTCETGEGFSASACNRKIIGA 187
           + +     DV++GVLDTG+WPE  S+SDEG  P+P+SWKG CE G  F+AS CNRK+IGA
Sbjct: 127 FPEAGSYSDVVVGVLDTGVWPESKSYSDEGFGPIPSSWKGGCEAGTNFTASLCNRKLIGA 186

Query: 188 RAYFYGYESNLGRSLRSSDFKSARDTDGHGTHTASTAAGSFVKNASFFQYARGEARGMAS 247
           R +  GYES +G    S + +S RD DGHGTHT+STAAGS V+ AS   YA G ARGMA 
Sbjct: 187 RFFARGYESTMGPIDESKESRSPRDDDGHGTHTSSTAAGSVVEGASLLGYASGTARGMAP 246

Query: 248 RARIAAYKICWEYGCFDSDILAAMDQAIADGVHIISLSVGSSGRAPAYYRDSIAIGAFGA 307
           RAR+A YK+CW  GCF SDILAA+D+AIAD V+++S+S+G  G    YYRD +AIGAF A
Sbjct: 247 RARVAVYKVCWLGGCFSSDILAAIDKAIADNVNVLSMSLG--GGMSDYYRDGVAIGAFAA 306

Query: 308 MQHGVVVSCSAGNSGPGSYTAVNIAPWILTVGASTIDREFLADVILGDGRVFSGVSLYSG 367
           M+ G++VSCSAGN+GP S +  N+APWI TVGA T+DR+F A  ILG+G+ F+GVSL+ G
Sbjct: 307 MERGILVSCSAGNAGPSSSSLSNVAPWITTVGAGTLDRDFPALAILGNGKNFTGVSLFKG 366

Query: 368 EPLGDSKLPLVYGGDCGS----RYCYSGSLDSSKVAGKIVVCDRGGNARVAKGSAVKSAG 427
           E L D  LP +Y G+  +      C +G+L   KV GKIV+CDRG NARV KG  VK+AG
Sbjct: 367 EALPDKLLPFIYAGNASNATNGNLCMTGTLIPEKVKGKIVMCDRGINARVQKGDVVKAAG 426

Query: 428 GLGMVLANTEENGEELLADSHLIPGTMVGEIAGNKLRNYTHSDPNPTATIVFRGTVIGNS 487
           G+GM+LANT  NGEEL+AD+HL+P T VGE AG+ +R+Y  +DPNPTA+I   GTV+G  
Sbjct: 427 GVGMILANTAANGEELVADAHLLPATTVGEKAGDIIRHYVTTDPNPTASISILGTVVGVK 486

Query: 488 PSAPRVAAFSSRGPNYRTAEILKPDVIAPGVNILAGWSGYSSPTSLSIDPRRVEFNIISG 547
           PS P VAAFSSRGPN  T  ILKPD+IAPGVNILA W+G + PT L+ D RRVEFNIISG
Sbjct: 487 PS-PVVAAFSSRGPNSITPNILKPDLIAPGVNILAAWTGAAGPTGLASDSRRVEFNIISG 546

Query: 548 TSMSCPHVSGVAALLRKAFPTWSPAAIKSALITTSYSLDSSGSPIKDLSTSKESNPFVHG 607
           TSMSCPHVSG+AALL+   P WSPAAI+SAL+TT+Y     G P+ D++T K S PF HG
Sbjct: 547 TSMSCPHVSGLAALLKSVHPEWSPAAIRSALMTTAYKTYKDGKPLLDIATGKPSTPFDHG 606

Query: 608 AGHIDPNKALNPGLIYDLTPQDYVSFLCSIGYDSRQIAVFVKDSSYSQLCE-HKLSNPGN 667
           AGH+ P  A NPGLIYDLT +DY+ FLC++ Y S QI      S  +  C+  K  +  +
Sbjct: 607 AGHVSPTTATNPGLIYDLTTEDYLGFLCALNYTSPQIR---SVSRRNYTCDPSKSYSVAD 666

Query: 668 LNYPSFAVVFDDEEVVKYTRTVTNVGDETDAVYEVKVES-PQGVEISVVPNKLEFNEKKT 727
           LNYPSFAV  D     KYTRTVT+VG      Y VKV S   GV+ISV P  L F E   
Sbjct: 667 LNYPSFAVNVDGVGAYKYTRTVTSVGGA--GTYSVKVTSETTGVKISVEPAVLNFKEANE 726

Query: 728 TQSYEITFT----KISGFKELASFGSIQWSDGSHNVRSPIAVSF 761
            +SY +TFT    K SG     SFGSI+WSDG H V SP+A+S+
Sbjct: 727 KKSYTVTFTVDSSKPSGSN---SFGSIEWSDGKHVVGSPVAISW 756

BLAST of Lsi02G028920 vs. Swiss-Prot
Match: SBT18_ARATH (Subtilisin-like protease SBT1.8 OS=Arabidopsis thaliana GN=SBT1.8 PE=2 SV=1)

HSP 1 Score: 749.2 bits (1933), Expect = 4.5e-215
Identity = 395/738 (53.52%), Postives = 504/738 (68.29%), Query Frame = 1

Query: 30  QETFIVHVSKSEKPSLFSSHNHWHSSILESLSPSPHPTKLLYSYERAVNGFSASITAAQA 89
           ++T+I+ V+ S+KP  F +H+ W++S L S S       LLY+Y  + +GFSA + + +A
Sbjct: 27  KKTYIIRVNHSDKPESFLTHHDWYTSQLNSES------SLLYTYTTSFHGFSAYLDSTEA 86

Query: 90  AELRHVPG-VISVIPDRIRQLHTTRTPHFLGLADNLGLWADTNYAEDVIIGVLDTGIWPE 149
             L      ++ +  D +  LHTTRTP FLGL    G+    + +  VIIGVLDTG+WPE
Sbjct: 87  DSLLSSSNSILDIFEDPLYTLHTTRTPEFLGLNSEFGVHDLGSSSNGVIIGVLDTGVWPE 146

Query: 150 RPSFSDEGLSPVPASWKGTCETGEGFSASACNRKIIGARAYFYGYESNLGRSLRSS-DFK 209
             SF D  +  +P+ WKG CE+G  F +  CN+K+IGAR++  G++   G    S  +  
Sbjct: 147 SRSFDDTDMPEIPSKWKGECESGSDFDSKLCNKKLIGARSFSKGFQMASGGGFSSKRESV 206

Query: 210 SARDTDGHGTHTASTAAGSFVKNASFFQYARGEARGMASRARIAAYKICWEYGCFDSDIL 269
           S RD DGHGTHT++TAAGS V+NASF  YA G ARGMA+RAR+A YK+CW  GCF SDIL
Sbjct: 207 SPRDVDGHGTHTSTTAAGSAVRNASFLGYAAGTARGMATRARVATYKVCWSTGCFGSDIL 266

Query: 270 AAMDQAIADGVHIISLSVGSSGRAPAYYRDSIAIGAFGAMQHGVVVSCSAGNSGPGSYTA 329
           AAMD+AI DGV ++SLS+G  G AP YYRD+IAIGAF AM+ GV VSCSAGNSGP   + 
Sbjct: 267 AAMDRAILDGVDVLSLSLGG-GSAP-YYRDTIAIGAFSAMERGVFVSCSAGNSGPTRASV 326

Query: 330 VNIAPWILTVGASTIDREFLADVILGDGRVFSGVSLYSGEPLGDSKLPLVY--GGDCGSR 389
            N+APW++TVGA T+DR+F A   LG+G+  +GVSLYSG  +G   L LVY  G    S 
Sbjct: 327 ANVAPWVMTVGAGTLDRDFPAFANLGNGKRLTGVSLYSGVGMGTKPLELVYNKGNSSSSN 386

Query: 390 YCYSGSLDSSKVAGKIVVCDRGGNARVAKGSAVKSAGGLGMVLANTEENGEELLADSHLI 449
            C  GSLDSS V GKIVVCDRG NARV KG+ V+ AGGLGM++ANT  +GEEL+ADSHL+
Sbjct: 387 LCLPGSLDSSIVRGKIVVCDRGVNARVEKGAVVRDAGGLGMIMANTAASGEELVADSHLL 446

Query: 450 PGTMVGEIAGNKLRNYTHSDPNPTATIVFRGTVIGNSPSAPRVAAFSSRGPNYRTAEILK 509
           P   VG+  G+ LR Y  SD  PTA +VF+GTV+   PS P VAAFSSRGPN  T EILK
Sbjct: 447 PAIAVGKKTGDLLREYVKSDSKPTALLVFKGTVLDVKPS-PVVAAFSSRGPNTVTPEILK 506

Query: 510 PDVIAPGVNILAGWSGYSSPTSLSIDPRRVEFNIISGTSMSCPHVSGVAALLRKAFPTWS 569
           PDVI PGVNILAGWS    PT L  D RR +FNI+SGTSMSCPH+SG+A LL+ A P WS
Sbjct: 507 PDVIGPGVNILAGWSDAIGPTGLDKDSRRTQFNIMSGTSMSCPHISGLAGLLKAAHPEWS 566

Query: 570 PAAIKSALITTSYSLDSSGSPIKDLSTSKESNPFVHGAGHIDPNKALNPGLIYDLTPQDY 629
           P+AIKSAL+TT+Y LD++ +P+ D + +  SNP+ HG+GH+DP KAL+PGL+YD++ ++Y
Sbjct: 567 PSAIKSALMTTAYVLDNTNAPLHDAADNSLSNPYAHGSGHVDPQKALSPGLVYDISTEEY 626

Query: 630 VSFLCSIGYDSRQIAVFVKDSSYSQLCEHKLSNPGNLNYPSFAVVFDDEEVVKYTRTVTN 689
           + FLCS+ Y    I   VK  S +  C  K S+PG LNYPSF+V+F  + VV+YTR VTN
Sbjct: 627 IRFLCSLDYTVDHIVAIVKRPSVN--CSKKFSDPGQLNYPSFSVLFGGKRVVRYTREVTN 686

Query: 690 VGDETDAVYEVKVESPQGVEISVVPNKLEFNEKKTTQSYEITFTKISG--FKELASFGSI 749
           VG    +VY+V V     V ISV P+KL F      + Y +TF    G      A FGSI
Sbjct: 687 VG-AASSVYKVTVNGAPSVGISVKPSKLSFKSVGEKKRYTVTFVSKKGVSMTNKAEFGSI 746

Query: 750 QWSDGSHNVRSPIAVSFN 762
            WS+  H VRSP+A S+N
Sbjct: 747 TWSNPQHEVRSPVAFSWN 752

BLAST of Lsi02G028920 vs. Swiss-Prot
Match: SBT15_ARATH (Subtilisin-like protease SBT1.5 OS=Arabidopsis thaliana GN=SBT1.5 PE=2 SV=1)

HSP 1 Score: 738.0 bits (1904), Expect = 1.0e-211
Identity = 391/774 (50.52%), Postives = 519/774 (67.05%), Query Frame = 1

Query: 11  FFFFILCFVPVIFSRSPDNQETFIVHVSKSEKPSLFSSHNHWHSSILESLSPSPHPTKLL 70
           +FFF+L       S S  N  T+IVHV    KPS+F +H HW++S L SL+ SP    ++
Sbjct: 6   YFFFLLTLSSPSSSASSSNSLTYIVHVDHEAKPSIFPTHFHWYTSSLASLTSSP--PSII 65

Query: 71  YSYERAVNGFSASITAAQAAELRHVPGVISVIPDRIRQLHTTRTPHFLGL--ADNLGLWA 130
           ++Y+   +GFSA +T+  A++L   P VISVIP+++R LHTTR+P FLGL   D  GL  
Sbjct: 66  HTYDTVFHGFSARLTSQDASQLLDHPHVISVIPEQVRHLHTTRSPEFLGLRSTDKAGLLE 125

Query: 131 DTNYAEDVIIGVLDTGIWPERPSFSDEGLSPVPASWKGTCETGEGFSASACNRKIIGARA 190
           ++++  D++IGV+DTG+WPERPSF D GL PVP  WKG C   + F  SACNRK++GAR 
Sbjct: 126 ESDFGSDLVIGVIDTGVWPERPSFDDRGLGPVPIKWKGQCIASQDFPESACNRKLVGARF 185

Query: 191 YFYGYESNLGRSLRSSDFKSARDTDGHGTHTASTAAGSFVKNASFFQYARGEARGMASRA 250
           +  GYE+  G+   +++F+S RD+DGHGTHTAS +AG +V  AS   YA G A GMA +A
Sbjct: 186 FCGGYEATNGKMNETTEFRSPRDSDGHGTHTASISAGRYVFPASTLGYAHGVAAGMAPKA 245

Query: 251 RIAAYKICWEYGCFDSDILAAMDQAIADGVHIISLSVGSSGRAPAYYRDSIAIGAFGAMQ 310
           R+AAYK+CW  GC+DSDILAA D A+ADGV +ISLSVG  G    YY D+IAIGAFGA+ 
Sbjct: 246 RLAAYKVCWNSGCYDSDILAAFDTAVADGVDVISLSVG--GVVVPYYLDAIAIGAFGAID 305

Query: 311 HGVVVSCSAGNSGPGSYTAVNIAPWILTVGASTIDREFLADVILGDGRVFSGVSLYSGEP 370
            G+ VS SAGN GPG+ T  N+APW+ TVGA TIDR+F A+V LG+G++ SGVS+Y G  
Sbjct: 306 RGIFVSASAGNGGPGALTVTNVAPWMTTVGAGTIDRDFPANVKLGNGKMISGVSVYGGPG 365

Query: 371 LGDSKL-PLVYGGDC------GSRYCYSGSLDSSKVAGKIVVCDRGGNARVAKGSAVKSA 430
           L   ++ PLVYGG         S  C  GSLD + V GKIV+CDRG N+R  KG  V+  
Sbjct: 366 LDPGRMYPLVYGGSLLGGDGYSSSLCLEGSLDPNLVKGKIVLCDRGINSRATKGEIVRKN 425

Query: 431 GGLGMVLANTEENGEELLADSHLIPGTMVGEIAGNKLRNY------THSDPNPTATIVFR 490
           GGLGM++AN   +GE L+AD H++P T VG   G+++R Y      + S  +PTATIVF+
Sbjct: 426 GGLGMIIANGVFDGEGLVADCHVLPATSVGASGGDEIRRYISESSKSRSSKHPTATIVFK 485

Query: 491 GTVIGNSPSAPRVAAFSSRGPNYRTAEILKPDVIAPGVNILAGWSGYSSPTSLSIDPRRV 550
           GT +G  P AP VA+FS+RGPN  T EILKPDVIAPG+NILA W     P+ ++ D RR 
Sbjct: 486 GTRLGIRP-APVVASFSARGPNPETPEILKPDVIAPGLNILAAWPDRIGPSGVTSDNRRT 545

Query: 551 EFNIISGTSMSCPHVSGVAALLRKAFPTWSPAAIKSALITTSYSLDSSGSPIKDLSTSKE 610
           EFNI+SGTSM+CPHVSG+AALL+ A P WSPAAI+SALITT+Y++D+SG P+ D ST   
Sbjct: 546 EFNILSGTSMACPHVSGLAALLKAAHPDWSPAAIRSALITTAYTVDNSGEPMMDESTGNT 605

Query: 611 SNPFVHGAGHIDPNKALNPGLIYDLTPQDYVSFLCSIGYDSRQIAVFVKDSSYSQLCE-- 670
           S+   +G+GH+ P KA++PGL+YD+T  DY++FLC+  Y    I    +  +    C+  
Sbjct: 606 SSVMDYGSGHVHPTKAMDPGLVYDITSYDYINFLCNSNYTRTNIVTITRRQAD---CDGA 665

Query: 671 HKLSNPGNLNYPSFAVVF----DDEEVVKYTRTVTNVGDETDAVYEVKVESPQGVEISVV 730
            +  + GNLNYPSF+VVF    + +    + RTVTNVGD +D+VYE+K+  P+G  ++V 
Sbjct: 666 RRAGHVGNLNYPSFSVVFQQYGESKMSTHFIRTVTNVGD-SDSVYEIKIRPPRGTTVTVE 725

Query: 731 PNKLEFNEKKTTQSYEI----TFTKISGFKELASFGSIQWSDGSHNVRSPIAVS 760
           P KL F       S+ +    T  K+S        G I WSDG  NV SP+ V+
Sbjct: 726 PEKLSFRRVGQKLSFVVRVKTTEVKLSPGATNVETGHIVWSDGKRNVTSPLVVT 770

BLAST of Lsi02G028920 vs. Swiss-Prot
Match: SBT13_ARATH (Subtilisin-like protease SBT1.3 OS=Arabidopsis thaliana GN=SBT1.3 PE=2 SV=1)

HSP 1 Score: 715.3 bits (1845), Expect = 7.1e-205
Identity = 380/773 (49.16%), Postives = 512/773 (66.24%), Query Frame = 1

Query: 12  FFFILCFVPVIFSRSP-----DNQETFIVHVSKSEKPSLFSSHNHWHSSILESLSPSP-- 71
           F FI+  + +IF ++        ++T+++H+ KS  P  +++H  W+SS + S++     
Sbjct: 11  FLFIILSINLIFLQAETTTQISTKKTYVIHMDKSAMPLPYTNHLQWYSSKINSVTQHKSQ 70

Query: 72  ----HPTKLLYSYERAVNGFSASITAAQAAELRHVPGVISVIPDRIRQLHTTRTPHFLGL 131
               +  ++LY+Y+ A +G +A +T  +A  L    GV++VIP+   +LHTTR+P FLGL
Sbjct: 71  EEEGNNNRILYTYQTAFHGLAAQLTQEEAERLEEEDGVVAVIPETRYELHTTRSPTFLGL 130

Query: 132 A--DNLGLWADTNYAEDVIIGVLDTGIWPERPSFSDEGLSPVPASWKGTCETGEGFSASA 191
              ++  +WA+     DV++GVLDTGIWPE  SF+D G+SPVPA+W+G CETG+ F    
Sbjct: 131 ERQESERVWAERVTDHDVVVGVLDTGIWPESESFNDTGMSPVPATWRGACETGKRFLKRN 190

Query: 192 CNRKIIGARAYFYGYESNLGRSLRSSDFKSARDTDGHGTHTASTAAGSFVKNASFFQYAR 251
           CNRKI+GAR ++ GYE+  G+     ++KS RD DGHGTHTA+T AGS VK A+ F +A 
Sbjct: 191 CNRKIVGARVFYRGYEAATGKIDEELEYKSPRDRDGHGTHTAATVAGSPVKGANLFGFAY 250

Query: 252 GEARGMASRARIAAYKICWEYGCFDSDILAAMDQAIADGVHIISLSVGSSGRAPAYYRDS 311
           G ARGMA +AR+AAYK+CW  GCF SDIL+A+DQA+ADGV ++S+S+G  G    Y RDS
Sbjct: 251 GTARGMAQKARVAAYKVCWVGGCFSSDILSAVDQAVADGVQVLSISLG--GGVSTYSRDS 310

Query: 312 IAIGAFGAMQHGVVVSCSAGNSGPGSYTAVNIAPWILTVGASTIDREFLADVILGDGRVF 371
           ++I  FGAM+ GV VSCSAGN GP   +  N++PWI TVGAST+DR+F A V +G  R F
Sbjct: 311 LSIATFGAMEMGVFVSCSAGNGGPDPISLTNVSPWITTVGASTMDRDFPATVKIGTMRTF 370

Query: 372 SGVSLYSGEPL--GDSKLPLVYGGDCGS-----RYCYSGSLDSSKVAGKIVVCDRGGNAR 431
            GVSLY G  +   + + PLVY G   S      +C  G+LD   VAGKIV+CDRG   R
Sbjct: 371 KGVSLYKGRTVLPKNKQYPLVYLGRNASSPDPTSFCLDGALDRRHVAGKIVICDRGVTPR 430

Query: 432 VAKGSAVKSAGGLGMVLANTEENGEELLADSHLIPGTMVGEIAGNKLRNYTHSDPNPTAT 491
           V KG  VK AGG+GMVL NT  NGEEL+ADSH++P   VGE  G  ++ Y  +    TA+
Sbjct: 431 VQKGQVVKRAGGIGMVLTNTATNGEELVADSHMLPAVAVGEKEGKLIKQYAMTSKKATAS 490

Query: 492 IVFRGTVIGNSPSAPRVAAFSSRGPNYRTAEILKPDVIAPGVNILAGWSGYSSPTSLSID 551
           +   GT IG  PS P VAAFSSRGPN+ + EILKPD++APGVNILA W+G  +P+SLS D
Sbjct: 491 LEILGTRIGIKPS-PVVAAFSSRGPNFLSLEILKPDLLAPGVNILAAWTGDMAPSSLSSD 550

Query: 552 PRRVEFNIISGTSMSCPHVSGVAALLRKAFPTWSPAAIKSALITTSYSLDSSGSPIKDLS 611
           PRRV+FNI+SGTSMSCPHVSGVAAL++   P WSPAAIKSAL+TT+Y  D+   P+ D S
Sbjct: 551 PRRVKFNILSGTSMSCPHVSGVAALIKSRHPDWSPAAIKSALMTTAYVHDNMFKPLTDAS 610

Query: 612 TSKESNPFVHGAGHIDPNKALNPGLIYDLTPQDYVSFLCSIGYDSRQIAVFVKDSSYSQL 671
            +  S+P+ HGAGHIDP +A +PGL+YD+ PQ+Y  FLC+      Q+ VF K S  ++ 
Sbjct: 611 GAAPSSPYDHGAGHIDPLRATDPGLVYDIGPQEYFEFLCTQDLSPSQLKVFTKHS--NRT 670

Query: 672 CEHKLS-NPGNLNYPSFAVVFDDEEVVK---YTRTVTNVGDETDAVYEVKVESPQGVEIS 731
           C+H L+ NPGNLNYP+ + +F +   VK     RTVTNVG    + Y+V V   +G  ++
Sbjct: 671 CKHTLAKNPGNLNYPAISALFPENTHVKAMTLRRTVTNVGPHISS-YKVSVSPFKGASVT 730

Query: 732 VVPNKLEFNEKKTTQSYEITFTKISGFKELASFGSIQWSDGSHNVRSPIAVSF 761
           V P  L F  K    SY +TF      K    FG + W   +H VRSP+ +++
Sbjct: 731 VQPKTLNFTSKHQKLSYTVTFRTRFRMKR-PEFGGLVWKSTTHKVRSPVIITW 776

BLAST of Lsi02G028920 vs. TrEMBL
Match: A0A0A0K6Z1_CUCSA (Subtilase family protein OS=Cucumis sativus GN=Csa_7G448910 PE=4 SV=1)

HSP 1 Score: 1388.2 bits (3592), Expect = 0.0e+00
Identity = 699/771 (90.66%), Postives = 733/771 (95.07%), Query Frame = 1

Query: 1   MANFHL-CSSLFFFFILCFVPVIFSRSPDNQETFIVHVSKSEKPSLFSSHNHWHSSILES 60
           MANFHL  SS FFFF+ CF P+IFSRS +NQET+IVHVSKSEKPSLFSSH+HWHSSILES
Sbjct: 1   MANFHLPSSSFFFFFLFCFFPLIFSRSLENQETYIVHVSKSEKPSLFSSHHHWHSSILES 60

Query: 61  LSPSPHPTKLLYSYERAVNGFSASITAAQAAELRHVPGVISVIPDRIRQLHTTRTPHFLG 120
           LS SPHPTKLLY+YERA NGFSA IT  QA ELR VPG+ISVIPD+IRQLHTTRTPHFLG
Sbjct: 61  LSSSPHPTKLLYNYERAANGFSARITTVQAEELRRVPGIISVIPDQIRQLHTTRTPHFLG 120

Query: 121 LADNLGLWADTNYAEDVIIGVLDTGIWPERPSFSDEGLSPVPASWKGTCETGEGFSASAC 180
           LADNLGLWADTNYA+DVIIGVLDTGIWPERPSFSDEGLSPVPA WKGTC+TGEG SA AC
Sbjct: 121 LADNLGLWADTNYADDVIIGVLDTGIWPERPSFSDEGLSPVPARWKGTCDTGEGVSAFAC 180

Query: 181 NRKIIGARAYFYGYESNLGRSLR-SSDFKSARDTDGHGTHTASTAAGSFVKNASFFQYAR 240
           NRKIIGARAYFYGYESNL  SL+ SSDFKSARDT+GHGTHTASTAAGSFV NASFFQYAR
Sbjct: 181 NRKIIGARAYFYGYESNLRGSLKVSSDFKSARDTEGHGTHTASTAAGSFVNNASFFQYAR 240

Query: 241 GEARGMASRARIAAYKICWEYGCFDSDILAAMDQAIADGVHIISLSVGSSGRAPAYYRDS 300
           GEARGMASRARIAAYKICWE+GC+DSDILAAMDQAI+DGV +ISLSVGSSGRAPAYYRDS
Sbjct: 241 GEARGMASRARIAAYKICWEFGCYDSDILAAMDQAISDGVDVISLSVGSSGRAPAYYRDS 300

Query: 301 IAIGAFGAMQHGVVVSCSAGNSGPGSYTAVNIAPWILTVGASTIDREFLADVILGDGRVF 360
           IAIGAFGAMQHGVVVSCSAGNSGPG YTAVNIAPWILTVGASTIDREFLADVILGDGRVF
Sbjct: 301 IAIGAFGAMQHGVVVSCSAGNSGPGPYTAVNIAPWILTVGASTIDREFLADVILGDGRVF 360

Query: 361 SGVSLYSGEPLGDSKLPLVYGGDCGSRYCYSGSLDSSKVAGKIVVCDRGGNARVAKGSAV 420
           SGVSLYSG+PLGDSKL LVYGGDCGSRYCYSGSLDSSKVAGKIVVCDRGGNARVAKG AV
Sbjct: 361 SGVSLYSGDPLGDSKLQLVYGGDCGSRYCYSGSLDSSKVAGKIVVCDRGGNARVAKGGAV 420

Query: 421 KSAGGLGMVLANTEENGEELLADSHLIPGTMVGEIAGNKLRNYTHSDPNPTATIVFRGTV 480
           KSAGGLGMVLANTEENGEELLADSHLIPGTMVG IAGNKLR+Y H+DPNPTATIVFRGTV
Sbjct: 421 KSAGGLGMVLANTEENGEELLADSHLIPGTMVGAIAGNKLRDYIHTDPNPTATIVFRGTV 480

Query: 481 IGNSPSAPRVAAFSSRGPNYRTAEILKPDVIAPGVNILAGWSGYSSPTSLSIDPRRVEFN 540
           IG+SP APRVAAFSSRGPNYRTAEILKPDVIAPGVNILAGWSGYSSPT L+IDPRRVEFN
Sbjct: 481 IGDSPPAPRVAAFSSRGPNYRTAEILKPDVIAPGVNILAGWSGYSSPTGLNIDPRRVEFN 540

Query: 541 IISGTSMSCPHVSGVAALLRKAFPTWSPAAIKSALITTSYSLDSSGSPIKDLSTSKESNP 600
           IISGTSMSCPHVSGVAALLRKAFPTWSPAAIKSALITTSYSLDSSG PIKDLSTS+ESNP
Sbjct: 541 IISGTSMSCPHVSGVAALLRKAFPTWSPAAIKSALITTSYSLDSSGKPIKDLSTSEESNP 600

Query: 601 FVHGAGHIDPNKALNPGLIYDLTPQDYVSFLCSIGYDSRQIAVFVKDSSYSQLCEHKLSN 660
           FVHGAGHI+PN+ALNPGLIYDLTPQDYVSFLCSIGYDS+QIAVFVK SSY QLCEHKL+N
Sbjct: 601 FVHGAGHINPNQALNPGLIYDLTPQDYVSFLCSIGYDSKQIAVFVKGSSYFQLCEHKLTN 660

Query: 661 PGNLNYPSFAVVFDDEEVVKYTRTVTNVGDETDAVYEVKVESPQGVEISVVPNKLEFNEK 720
           PGNLNYPSF+VVFD+EEVVKYTRTVTNVGDET+ VYEVKVE+PQGV ISVVPNKLEFN++
Sbjct: 661 PGNLNYPSFSVVFDEEEVVKYTRTVTNVGDETEVVYEVKVEAPQGVVISVVPNKLEFNKE 720

Query: 721 KTTQSYEITFTKISGFKELASFGSIQWSDGSHNVRSPIAVSFNT-GSIASM 769
           KTTQSYEITFTKI+GFKE ASFGSIQW DG H+VRSPIAVSF T GSIASM
Sbjct: 721 KTTQSYEITFTKINGFKESASFGSIQWGDGIHSVRSPIAVSFKTGGSIASM 771

BLAST of Lsi02G028920 vs. TrEMBL
Match: M1A1D2_SOLTU (Uncharacterized protein OS=Solanum tuberosum GN=PGSC0003DMG400004891 PE=4 SV=1)

HSP 1 Score: 1068.5 bits (2762), Expect = 3.7e-309
Identity = 537/778 (69.02%), Postives = 631/778 (81.11%), Query Frame = 1

Query: 1   MANFHLCSSLFFFFILCFVPVIFSRSPDNQETFIVHVSKSEKPSLFSSHNHWHSSILESL 60
           MA   + SS+FF    C  PV  S   D  ETFI+HV+KS+KP +FS+H+HW+SSI+ S+
Sbjct: 1   MAKISVLSSIFFIISFCLTPVAISVQSDGHETFIIHVAKSDKPHVFSTHHHWYSSIVRSI 60

Query: 61  SP-SPHPTKLLYSYERAVNGFSASITAAQAAELRHVPGVISVIPDRIRQLHTTRTPHFLG 120
           SP S H +K+LY+YERA  GFSA +TA QA +LR VPGVISVIPD++R LHTT TP FL 
Sbjct: 61  SPPSHHRSKILYTYERAAVGFSARLTAGQADQLRRVPGVISVIPDQVRYLHTTHTPTFLK 120

Query: 121 LADNLGLWADTNYAEDVIIGVLDTGIWPERPSFSDEGLSPVPASWKGTCETGEGFSASAC 180
           LAD+ GLW D++YA+DVI+GVLDTGIWPERPSFSDEGLSPVPA WKG C TG GF  S+C
Sbjct: 121 LADSFGLWPDSDYADDVIVGVLDTGIWPERPSFSDEGLSPVPAGWKGKCVTGPGFPRSSC 180

Query: 181 NRKIIGARAYFYGYESNLGRSLRSSDFKSARDTDGHGTHTASTAAGSFVKNASFFQYARG 240
           NRKIIGAR ++ GYE++ G    S + KS RDT+GHGTHTASTAAGS V NASF+QYA+G
Sbjct: 181 NRKIIGARMFYKGYEASHGPMDESKEAKSPRDTEGHGTHTASTAAGSLVANASFYQYAKG 240

Query: 241 EARGMASRARIAAYKICWEYGCFDSDILAAMDQAIADGVHIISLSVGSSGRAPAYYRDSI 300
           EARGMA +ARIAAYKICW+ GCFDSDILAAMDQA+ADGVH+ISLSVG++G AP Y  DSI
Sbjct: 241 EARGMAIKARIAAYKICWKDGCFDSDILAAMDQAVADGVHVISLSVGANGYAPHYLHDSI 300

Query: 301 AIGAFGAMQHGVVVSCSAGNSGPGSYTAVNIAPWILTVGASTIDREFLADVILGDGRVFS 360
           AIGAFGA +HGV+VSCSAGNSGPG YTAVNIAPWILTVGASTIDREF ADVILGD RVF 
Sbjct: 301 AIGAFGASEHGVLVSCSAGNSGPGPYTAVNIAPWILTVGASTIDREFPADVILGDDRVFG 360

Query: 361 GVSLYSGEPLGDSKLPLVYGGDCGSRYCYSGSLDSSKVAGKIVVCDRGGNARVAKGSAVK 420
           GVSLYSG PL DSK P+VY GDCGS+YCY G LD  KVAGKIV+CDRGGNARV KGSAVK
Sbjct: 361 GVSLYSGNPLTDSKFPVVYSGDCGSKYCYPGKLDHKKVAGKIVLCDRGGNARVEKGSAVK 420

Query: 421 SAGGLGMVLANTEENGEELLADSHLIPGTMVGEIAGNKLRNYTHSDPNPTATIVFRGTVI 480
            AGG+GM+LAN  E+GEEL+ADSHL+P TMVG+ AG+K+R Y  SD +PTATIVFRGTVI
Sbjct: 421 LAGGVGMILANLAESGEELVADSHLLPATMVGQKAGDKIREYVTSDTSPTATIVFRGTVI 480

Query: 481 GNSPSAPRVAAFSSRGPNYRTAEILKPDVIAPGVNILAGWSGYSSPTSLSIDPRRVEFNI 540
           GNSP+APRVAAFSSRGPN+ T EILKPDVIAPGVNILAGW+G + PT L+IDPRRVEFNI
Sbjct: 481 GNSPAAPRVAAFSSRGPNHLTPEILKPDVIAPGVNILAGWTGSTGPTDLAIDPRRVEFNI 540

Query: 541 ISGTSMSCPHVSGVAALLRKAFPTWSPAAIKSALITTSYSLDSSGSPIKDLSTSKESNPF 600
           ISGTSMSCPHVSG+AALLR+A   W+PAAIKSAL+TT+Y+LD+SG    DL+T +ES PF
Sbjct: 541 ISGTSMSCPHVSGLAALLRRAHSKWTPAAIKSALMTTAYNLDNSGKIFTDLATGEESTPF 600

Query: 601 VHGAGHIDPNKALNPGLIYDLTPQDYVSFLCSIGYDSRQIAVFVKDSSYSQLCEHKLSNP 660
           VHG+GH+DPN+AL+PGL+YD+   DYV+FLC+IGYD   IAVFV+DSS     E  L+ P
Sbjct: 601 VHGSGHVDPNRALDPGLVYDIETSDYVNFLCTIGYDGDDIAVFVRDSSRVNCSERSLATP 660

Query: 661 GNLNYPSFAVVF--DDEEVVKYTRTVTNVGDETDAVYEVKVESPQGVEISVVPNKLEFNE 720
           G+LNYPSF+V F  D   VVKY R V NVG +++AVYEVKV +P  VE+SV P KL F+E
Sbjct: 661 GDLNYPSFSVDFTSDSNGVVKYKRVVKNVGGDSNAVYEVKVNAPSAVEVSVSPAKLVFSE 720

Query: 721 KKTTQSYEITFTK-------ISGFKELASFGSIQWSDGSHNVRSPIAVSFNTGSIASM 769
           +  + SYEI+FT        + G +  ++FGSI+WSDG H+VRSPIAV +   S  SM
Sbjct: 721 ENNSLSYEISFTSKRSEDIMVKGIQ--SAFGSIEWSDGIHSVRSPIAVRWRYQSAVSM 776

BLAST of Lsi02G028920 vs. TrEMBL
Match: A0A061GCZ9_THECC (Subtilase family protein OS=Theobroma cacao GN=TCM_029155 PE=4 SV=1)

HSP 1 Score: 1067.4 bits (2759), Expect = 8.2e-309
Identity = 541/760 (71.18%), Postives = 625/760 (82.24%), Query Frame = 1

Query: 9   SLFFFFILCFVPVIFSRSPDNQETFIVHVSKSEKPSLFSSHNHWHSSILESLSPSPHPTK 68
           S FFF  L F+P   S S D  E FI+HVSKS KPSLFSSH+HW+SSI+ SL PSPHPTK
Sbjct: 4   SFFFFLSLLFIP-FSSSSSDRPENFIIHVSKSHKPSLFSSHHHWYSSIIHSLPPSPHPTK 63

Query: 69  LLYSYERAVNGFSASITAAQAAELRHVPGVISVIPDRIRQLHTTRTPHFLGLADNLGLWA 128
           LLY+YERA+NGFSA +TA QA +LR +PG++SVIPD++RQ+HTTRTP FLGL+D +GLW 
Sbjct: 64  LLYTYERAINGFSARLTATQAEKLRELPGILSVIPDQVRQIHTTRTPQFLGLSDGVGLWQ 123

Query: 129 DTNYAEDVIIGVLDTGIWPERPSFSDEGLSPVPASWKGTCETGEGFSASACNRKIIGARA 188
           ++ Y + VIIGVLDTGIWPERPSF D GLSPVP SWKG CETG  F ASAC+RKIIGARA
Sbjct: 124 NSYYGDGVIIGVLDTGIWPERPSFKDSGLSPVPDSWKGICETGPDFPASACSRKIIGARA 183

Query: 189 YFYGYESNL-GRSLRSSDFKSARDTDGHGTHTASTAAGSFVKNASFFQYARGEARGMASR 248
           ++ GYES L G    + + KS RDT+GHGTHTASTAAGS V NAS F++A GEARGMA++
Sbjct: 184 FYKGYESYLEGPMDETKESKSPRDTEGHGTHTASTAAGSVVSNASLFEFAYGEARGMATK 243

Query: 249 ARIAAYKICWEYGCFDSDILAAMDQAIADGVHIISLSVGSSGRAPAYYRDSIAIGAFGAM 308
           ARIAAYKICW  GCFDSD+LAAMDQAIADGV++ISLSVG++G AP Y  DSIAIGAFGA 
Sbjct: 244 ARIAAYKICWSLGCFDSDLLAAMDQAIADGVNVISLSVGATGYAPQYDHDSIAIGAFGAA 303

Query: 309 QHGVVVSCSAGNSGPGSYTAVNIAPWILTVGASTIDREFLADVILGDGRVFSGVSLYSGE 368
           QHG+VVSCSAGNSGPG YTAVNIAPWILTVGASTIDREF AD ILGDGR+F GVSLYSG+
Sbjct: 304 QHGIVVSCSAGNSGPGPYTAVNIAPWILTVGASTIDREFPADAILGDGRIFGGVSLYSGD 363

Query: 369 PLGDSKLPLVYGGDCGSRYCYSGSLDSSKVAGKIVVCDRGGNARVAKGSAVKSAGGLGMV 428
           PL D KLPLVY GD G+RYCY GSL  SKV GKIV CDRGGNARV KG AVK AGGLGM+
Sbjct: 364 PLVDIKLPLVYAGDSGNRYCYMGSLSPSKVQGKIVFCDRGGNARVEKGFAVKLAGGLGMI 423

Query: 429 LANTEENGEELLADSHLIPGTMVGEIAGNKLRNYTHSDPNPTATIVFRGTVIGNSPSAPR 488
           LANT E+GEEL+AD+HLIP T VGE AGN++R Y      PTATIVF GTVIG SP AP+
Sbjct: 424 LANTAESGEELIADAHLIPATTVGEKAGNEIRQYIKISQFPTATIVFHGTVIGPSPPAPK 483

Query: 489 VAAFSSRGPNYRTAEILKPDVIAPGVNILAGWSGYSSPTSLSIDPRRVEFNIISGTSMSC 548
           VAAFSSRGPN+ T EILKPDVIAPGVNILAGW+G+  P+ L+ID RRV FNIISGTSMSC
Sbjct: 484 VAAFSSRGPNHLTPEILKPDVIAPGVNILAGWTGFIGPSQLNIDTRRVNFNIISGTSMSC 543

Query: 549 PHVSGVAALLRKAFPTWSPAAIKSALITTSYSLDSSGSPIKDLSTSKESNPFVHGAGHID 608
           PHVSG+AALL KA+P WSPAAIKSAL+TT+YSLD+SG+ IKDL+T  ES+PFV+GAGH+D
Sbjct: 544 PHVSGLAALLIKAYPNWSPAAIKSALMTTAYSLDNSGNTIKDLATGVESSPFVYGAGHVD 603

Query: 609 PNKALNPGLIYDLTPQDYVSFLCSIGYDSRQIAVFVKDSSYSQLCEHKLSNPGNLNYPSF 668
           PN AL PGL+YD+   DYV+FLCSIGYDS++IA+FV++ +   +CE KL+ PGNLNYPSF
Sbjct: 604 PNIALMPGLVYDIDDGDYVAFLCSIGYDSKRIAIFVREPTGPDVCEGKLATPGNLNYPSF 663

Query: 669 AVVFD-DEEVVKYTRTVTNVGDETDAVYEVKVESPQGVEISVVPNKLEFNEKKTTQSYEI 728
           +VVFD ++ VVKY RTV NVG   DAVYE KV +P GVEISV P+KLEF+ +  T SYEI
Sbjct: 664 SVVFDSNDHVVKYKRTVKNVGPSVDAVYEAKVNAPPGVEISVSPSKLEFSAENQTLSYEI 723

Query: 729 TFTK--ISGFK-ELASFGSIQWSDGSHNVRSPIAVSFNTG 764
           TF    ++ F   L +FGSI+WSDG H VRSPIAV +  G
Sbjct: 724 TFASDGLALFAVALEAFGSIEWSDGVHLVRSPIAVRWLQG 762

BLAST of Lsi02G028920 vs. TrEMBL
Match: A0A0D2QGD0_GOSRA (Uncharacterized protein OS=Gossypium raimondii GN=B456_009G146000 PE=4 SV=1)

HSP 1 Score: 1065.1 bits (2753), Expect = 4.1e-308
Identity = 529/759 (69.70%), Postives = 624/759 (82.21%), Query Frame = 1

Query: 11  FFFFILCFVPVIFSRSPDNQETFIVHVSKSEKPSLFSSHNHWHSSILESLSPSPHPTKLL 70
           FF  +LC +P   S S D  E FI+HVSKS KP LFSSH+HW+SSIL SL PSPHP KLL
Sbjct: 7   FFLSLLC-IP-FSSPSSDGPENFIIHVSKSHKPPLFSSHHHWYSSILASLPPSPHPIKLL 66

Query: 71  YSYERAVNGFSASITAAQAAELRHVPGVISVIPDRIRQLHTTRTPHFLGLADNLGLWADT 130
           Y+YER +NGFSA +TAAQA +LR +PG++SVIPD++RQ+HTTRTPHFLGL+D +GLW ++
Sbjct: 67  YTYERVINGFSARLTAAQADKLRGLPGILSVIPDQVRQIHTTRTPHFLGLSDGVGLWQNS 126

Query: 131 NYAEDVIIGVLDTGIWPERPSFSDEGLSPVPASWKGTCETGEGFSASACNRKIIGARAYF 190
            Y + VIIGVLDTGIWPERPSF+D  LSPVP +WKG CETG  F ASACNRK+IGARA++
Sbjct: 127 YYGDGVIIGVLDTGIWPERPSFTDSWLSPVPDTWKGICETGPDFPASACNRKVIGARAFY 186

Query: 191 YGYESNLGRSL-RSSDFKSARDTDGHGTHTASTAAGSFVKNASFFQYARGEARGMASRAR 250
            GY+S+LG S+  + +  S RDT+GHGTHTASTAAGS V NAS F +ARGEARGMAS+AR
Sbjct: 187 KGYQSHLGHSIDETKESTSPRDTEGHGTHTASTAAGSVVSNASLFGFARGEARGMASKAR 246

Query: 251 IAAYKICWEYGCFDSDILAAMDQAIADGVHIISLSVGSSGRAPAYYRDSIAIGAFGAMQH 310
           IAAYKICW +GCFDSDILAAMDQA ADGV +ISLSVG++G AP Y  DSIAIGAFGA  H
Sbjct: 247 IAAYKICWSFGCFDSDILAAMDQATADGVDVISLSVGATGYAPQYDHDSIAIGAFGAAAH 306

Query: 311 GVVVSCSAGNSGPGSYTAVNIAPWILTVGASTIDREFLADVILGDGRVFSGVSLYSGEPL 370
           G+VVSCSAGNSGPG+YTAVNIAPWI+TVGASTIDREF ADV+LGDGR+F GVSLY G PL
Sbjct: 307 GIVVSCSAGNSGPGTYTAVNIAPWIITVGASTIDREFPADVVLGDGRIFGGVSLYYGPPL 366

Query: 371 GDSKLPLVYGGDCGSRYCYSGSLDSSKVAGKIVVCDRGGNARVAKGSAVKSAGGLGMVLA 430
            D KL LVY GD G+RYCY G +  SKV GK+V+CDRGGNARV KG+AVK AGGLGM+ A
Sbjct: 367 PDFKLRLVYAGDAGNRYCYMGGISPSKVQGKMVLCDRGGNARVEKGAAVKLAGGLGMIQA 426

Query: 431 NTEENGEELLADSHLIPGTMVGEIAGNKLRNYTHSDPNPTATIVFRGTVIGNSPSAPRVA 490
           NT E+GEEL+AD+HL+P TMVGEIAGNK+R Y    P PTATI+FRGTV+G SP AP+VA
Sbjct: 427 NTAESGEELIADAHLVPATMVGEIAGNKIREYVKKSPFPTATILFRGTVVGPSPPAPKVA 486

Query: 491 AFSSRGPNYRTAEILKPDVIAPGVNILAGWSGYSSPTSLSIDPRRVEFNIISGTSMSCPH 550
           AFSSRGPN+ T EILKPDVIAPGVNILAGW+G+++PT L IDPRRV+FNIISGTSMSCPH
Sbjct: 487 AFSSRGPNHLTPEILKPDVIAPGVNILAGWTGFAAPTDLDIDPRRVDFNIISGTSMSCPH 546

Query: 551 VSGVAALLRKAFPTWSPAAIKSALITTSYSLDSSGSPIKDLSTSKESNPFVHGAGHIDPN 610
           VSG+AALL+KA+P WSPAAIKSAL+TT+Y+LD+SGS IKDL+T +ES+PF HGAGH+DPN
Sbjct: 547 VSGLAALLKKAYPKWSPAAIKSALMTTAYTLDNSGSTIKDLATGEESSPFGHGAGHVDPN 606

Query: 611 KALNPGLIYDLTPQDYVSFLCSIGYDSRQIAVFVKDSSYSQLCEHKLSNPGNLNYPSFAV 670
           +ALNPGL+YD+   DY++FLCSIGYD  +IAVFV+  + S +CE KL+ PG+LNYPSF+V
Sbjct: 607 RALNPGLVYDIDNDDYIAFLCSIGYDLNRIAVFVRGPTGSDICEGKLATPGDLNYPSFSV 666

Query: 671 VF-DDEEVVKYTRTVTNVGDETDAVYEVKVESPQGVEISVVPNKLEFNEKKTTQSYEITF 730
           VF  ++ VVKY RTV NVG   D VY  KV +P GVEI+V P KLEF+ +  T SYEITF
Sbjct: 667 VFYSNDHVVKYKRTVKNVGTSVDVVYTAKVNAPAGVEINVSPCKLEFSGENKTLSYEITF 726

Query: 731 TK----ISGFKELASFGSIQWSDGSHNVRSPIAVSFNTG 764
                 +     L +FGSI+WSDG H VRSPIAV +  G
Sbjct: 727 ASDGLGLHAVDSLQAFGSIEWSDGVHLVRSPIAVRWAQG 763

BLAST of Lsi02G028920 vs. TrEMBL
Match: M5XH18_PRUPE (Uncharacterized protein OS=Prunus persica GN=PRUPE_ppa001739mg PE=4 SV=1)

HSP 1 Score: 1064.3 bits (2751), Expect = 7.0e-308
Identity = 540/767 (70.40%), Postives = 631/767 (82.27%), Query Frame = 1

Query: 9   SLFFFFILCFVPVIFSRSPD---NQETFIVHVSKSEKPSLFSSHNHWHSSILESLSPSPH 68
           SLF F +   +P   S  PD     +TFIVHVSKS+KPSLFSSH  W++SI+++L PSPH
Sbjct: 7   SLFLFLLSLLLPATLSIPPDLSDRPKTFIVHVSKSQKPSLFSSHRSWYTSIIQNL-PSPH 66

Query: 69  PTKLLYSYERAVNGFSASITAAQAAELRHVPGVISVIPDRIRQLHTTRTPHFLGLADNLG 128
           PTKLLY+Y+R+V+GFSA++T++QA +L   P V+SV PD+ RQLHTT TP+FLGLAD+ G
Sbjct: 67  PTKLLYTYDRSVHGFSATLTSSQATQLLSHPSVLSVTPDQPRQLHTTHTPNFLGLADSFG 126

Query: 129 LWADTNYAEDVIIGVLDTGIWPERPSFSDEGLSPVPASWKGTCETGEGFSASACNRKIIG 188
           LW +++YA+DV+IGVLDTGIWPERPSFSD G+ PVP  WKGTC T   F +SACNRKIIG
Sbjct: 127 LWPNSDYADDVVIGVLDTGIWPERPSFSDSGIGPVPTRWKGTCVTTADFPSSACNRKIIG 186

Query: 189 ARAYFYGYESNLGRSL-RSSDFKSARDTDGHGTHTASTAAGSFVKNASFFQYARGEARGM 248
           ARAYF GYES++GR +  +++ KS RDT+GHGTHTASTAAG+ V NASFF YA+G+ARGM
Sbjct: 187 ARAYFNGYESHIGRLMDETTEAKSPRDTEGHGTHTASTAAGAVVANASFFSYAQGDARGM 246

Query: 249 ASRARIAAYKICWEYGCFDSDILAAMDQAIADGVHIISLSVGSSGRAPAYYRDSIAIGAF 308
           A++ARIA YKICW +GCFDSDILAAMDQAIADGV IISLSVG+SG AP Y RDSIAIGAF
Sbjct: 247 ATKARIAVYKICWSFGCFDSDILAAMDQAIADGVDIISLSVGASGNAPPYDRDSIAIGAF 306

Query: 309 GAMQHGVVVSCSAGNSGPGSYTAVNIAPWILTVGASTIDREFLADVILGDGRVFSGVSLY 368
           GA QHGV+VS SAGNSGP  +TA NIAPWILTVGASTIDREF ADV+LGD RV SGVSLY
Sbjct: 307 GAAQHGVLVSASAGNSGPNPFTATNIAPWILTVGASTIDREFPADVVLGDNRVISGVSLY 366

Query: 369 SGEPLGDSKLPLVYGGDCGSRYCYSGSLDSSKVAGKIVVCDRGGNARVAKGSAVKSAGGL 428
           SGEPL D KLPLVYGGDCGSRYCY G+L  SKV GKIVVCDRGGNARVAKG AVK AGGL
Sbjct: 367 SGEPLVDYKLPLVYGGDCGSRYCYEGALQPSKVQGKIVVCDRGGNARVAKGGAVKLAGGL 426

Query: 429 GMVLANTEENGEELLADSHLIPGTMVGEIAGNKLRNYTHSDPNPTATIVFRGTVIGNSPS 488
           GM+LANTEE+GEELLAD HLIP T VGEIA N++R Y      PTATIVFRGTVIG+SPS
Sbjct: 427 GMILANTEESGEELLADGHLIPATEVGEIAANQIREYIRLSQYPTATIVFRGTVIGSSPS 486

Query: 489 APRVAAFSSRGPNYRTAEILKPDVIAPGVNILAGWSGYSSPTSLSIDPRRVEFNIISGTS 548
           +P+VAAFSSRGPN  T EILKPDVIAPGVNILAGW+G ++PT L IDPRRVEFNIISGTS
Sbjct: 487 SPQVAAFSSRGPNSLTPEILKPDVIAPGVNILAGWTGATAPTDLDIDPRRVEFNIISGTS 546

Query: 549 MSCPHVSGVAALLRKAFPTWSPAAIKSALITTSYSLDSSGSPIKDLSTSKESNPFVHGAG 608
           MSCPHVSG+AALLRKAFP WS AAIKSALITT+Y+LD++G  IKDL T +ES PFVHGAG
Sbjct: 547 MSCPHVSGIAALLRKAFPNWSIAAIKSALITTAYTLDNAGKKIKDLGTGEESTPFVHGAG 606

Query: 609 HIDPNKALNPGLIYDLTPQDYVSFLCSIGYDSRQIAVFVKDSSYSQLC-EHKLSNPGNLN 668
           H+DPN+ALNPGLIYDL   DYV+FLCSIGY  RQIAVFV   + S +C  + L++PG+LN
Sbjct: 607 HVDPNRALNPGLIYDLNVNDYVAFLCSIGYSPRQIAVFVGKPTGSDMCTRNSLASPGDLN 666

Query: 669 YPSFAVVF-DDEEVVKYTRTVTNVGDETDAVYEVKVESPQGVEISVVPNKLEFNEKKTTQ 728
           YPSF+VV   D+ ++KY R  TNVG + DAVYEV V +P GVEISV P KL F+ +  TQ
Sbjct: 667 YPSFSVVLSSDQGLIKYKRIATNVGGDADAVYEVTVNAPAGVEISVEPRKLVFSAENQTQ 726

Query: 729 SYEITFTKISGFKELASFGSIQWSDGSHNVRSPIAVSFNTG-SIASM 769
           SYE+TF +  G+     +GSI+W+DG H VRSP+AV +++  S+ASM
Sbjct: 727 SYEVTFKRGVGYDGGERYGSIEWTDGRHLVRSPVAVRWSSARSLASM 772

BLAST of Lsi02G028920 vs. TAIR10
Match: AT3G14067.1 (AT3G14067.1 Subtilase family protein)

HSP 1 Score: 988.0 bits (2553), Expect = 3.2e-288
Identity = 500/771 (64.85%), Postives = 599/771 (77.69%), Query Frame = 1

Query: 8   SSLFFFF--ILCFVPVIFSRSPDNQETFIVHVSKSEKPSLFSSHNHWHSSILESLSPSPH 67
           SS+FF F  +LCF     S S D  E++IVHV +S KPSLFSSHN+WH S+L SL  SP 
Sbjct: 7   SSIFFVFPLLLCFFSPS-SSSSDGLESYIVHVQRSHKPSLFSSHNNWHVSLLRSLPSSPQ 66

Query: 68  PTKLLYSYERAVNGFSASITAAQAAELRHVPGVISVIPDRIRQLHTTRTPHFLGLADNLG 127
           P  LLYSY RAV+GFSA ++  Q A LR  P VISVIPD+ R++HTT TP FLG + N G
Sbjct: 67  PATLLYSYSRAVHGFSARLSPIQTAALRRHPSVISVIPDQAREIHTTHTPAFLGFSQNSG 126

Query: 128 LWADTNYAEDVIIGVLDTGIWPERPSFSDEGLSPVPASWKGTCETGEGFSASACNRKIIG 187
           LW+++NY EDVI+GVLDTGIWPE PSFSD GL P+P++WKG CE G  F AS+CNRK+IG
Sbjct: 127 LWSNSNYGEDVIVGVLDTGIWPEHPSFSDSGLGPIPSTWKGECEIGPDFPASSCNRKLIG 186

Query: 188 ARAYFYGY--ESNLGRSLRSSDFKSARDTDGHGTHTASTAAGSFVKNASFFQYARGEARG 247
           ARA++ GY  + N  +   + + +S RDT+GHGTHTASTAAGS V NAS +QYARG A G
Sbjct: 187 ARAFYRGYLTQRNGTKKHAAKESRSPRDTEGHGTHTASTAAGSVVANASLYQYARGTATG 246

Query: 248 MASRARIAAYKICWEYGCFDSDILAAMDQAIADGVHIISLSVGSSGRAPAYYRDSIAIGA 307
           MAS+ARIAAYKICW  GC+DSDILAAMDQA+ADGVH+ISLSVG+SG AP Y+ DSIAIGA
Sbjct: 247 MASKARIAAYKICWTGGCYDSDILAAMDQAVADGVHVISLSVGASGSAPEYHTDSIAIGA 306

Query: 308 FGAMQHGVVVSCSAGNSGPGSYTAVNIAPWILTVGASTIDREFLADVILGDGRVFSGVSL 367
           FGA +HG+VVSCSAGNSGP   TA NIAPWILTVGAST+DREF A+ I GDG+VF+G SL
Sbjct: 307 FGATRHGIVVSCSAGNSGPNPETATNIAPWILTVGASTVDREFAANAITGDGKVFTGTSL 366

Query: 368 YSGEPLGDSKLPLVYGGDCGSRYCYSGSLDSSKVAGKIVVCDRGGNARVAKGSAVKSAGG 427
           Y+GE L DS+L LVY GDCGSR CY G L+SS V GKIV+CDRGGNARV KGSAVK AGG
Sbjct: 367 YAGESLPDSQLSLVYSGDCGSRLCYPGKLNSSLVEGKIVLCDRGGNARVEKGSAVKLAGG 426

Query: 428 LGMVLANTEENGEELLADSHLIPGTMVGEIAGNKLRNYTHSDPNPTATIVFRGTVIGNSP 487
            GM+LANT E+GEEL ADSHL+P TMVG  AG+++R+Y  +  +PTA I F GT+IG SP
Sbjct: 427 AGMILANTAESGEELTADSHLVPATMVGAKAGDQIRDYIKTSDSPTAKISFLGTLIGPSP 486

Query: 488 SAPRVAAFSSRGPNYRTAEILKPDVIAPGVNILAGWSGYSSPTSLSIDPRRVEFNIISGT 547
            +PRVAAFSSRGPN+ T  ILKPDVIAPGVNILAGW+G   PT L IDPRRV+FNIISGT
Sbjct: 487 PSPRVAAFSSRGPNHLTPVILKPDVIAPGVNILAGWTGMVGPTDLDIDPRRVQFNIISGT 546

Query: 548 SMSCPHVSGVAALLRKAFPTWSPAAIKSALITTSYSLDSSGSPIKDLSTSKESNPFVHGA 607
           SMSCPHVSG+AALLRKA P WSPAAIKSAL+TT+Y +++SG PI+DL+T K SN F+HGA
Sbjct: 547 SMSCPHVSGLAALLRKAHPDWSPAAIKSALVTTAYDVENSGEPIEDLATGKSSNSFIHGA 606

Query: 608 GHIDPNKALNPGLIYDLTPQDYVSFLCSIGYDSRQIAVFVKDSSYSQLCE-HKLSNPGNL 667
           GH+DPNKALNPGL+YD+  ++YV+FLC++GY+   I VF++D +    CE  KL   G+L
Sbjct: 607 GHVDPNKALNPGLVYDIEVKEYVAFLCAVGYEFPGILVFLQDPTLYDACETSKLRTAGDL 666

Query: 668 NYPSFAVVF-DDEEVVKYTRTVTNVGDETDAVYEVKVESPQGVEISVVPNKLEFNEKKTT 727
           NYPSF+VVF    EVVKY R V NVG   DAVYEV V+SP  VEI V P+KL F+++K+ 
Sbjct: 667 NYPSFSVVFASTGEVVKYKRVVKNVGSNVDAVYEVGVKSPANVEIDVSPSKLAFSKEKSV 726

Query: 728 QSYEITFTKI-----SGFKELASFGSIQWSDGSHNVRSPIAVSFNTGSIAS 768
             YE+TF  +      G      FGSI+W+DG H V+SP+AV +  GS+ S
Sbjct: 727 LEYEVTFKSVVLGGGVGSVPGHEFGSIEWTDGEHVVKSPVAVQWGQGSVQS 776

BLAST of Lsi02G028920 vs. TAIR10
Match: AT5G67360.1 (AT5G67360.1 Subtilase family protein)

HSP 1 Score: 792.7 bits (2046), Expect = 2.0e-229
Identity = 426/764 (55.76%), Postives = 532/764 (69.63%), Query Frame = 1

Query: 8   SSLFFFFILCFVPVIFSRSPDNQETFIVHVSKSEKPSLFSSHNHWHSSILESLSPSPHPT 67
           SS  FF +LC      S S  +Q T+IVH++KS+ PS F  H++W+ S L S+S S    
Sbjct: 7   SSTAFFLLLCLGFCHVSSSSSDQGTYIVHMAKSQMPSSFDLHSNWYDSSLRSISDS---A 66

Query: 68  KLLYSYERAVNGFSASITAAQAAELRHVPGVISVIPDRIRQLHTTRTPHFLGLADNLG-L 127
           +LLY+YE A++GFS  +T  +A  L   PGVISV+P+   +LHTTRTP FLGL ++   L
Sbjct: 67  ELLYTYENAIHGFSTRLTQEEADSLMTQPGVISVLPEHRYELHTTRTPLFLGLDEHTADL 126

Query: 128 WADTNYAEDVIIGVLDTGIWPERPSFSDEGLSPVPASWKGTCETGEGFSASACNRKIIGA 187
           + +     DV++GVLDTG+WPE  S+SDEG  P+P+SWKG CE G  F+AS CNRK+IGA
Sbjct: 127 FPEAGSYSDVVVGVLDTGVWPESKSYSDEGFGPIPSSWKGGCEAGTNFTASLCNRKLIGA 186

Query: 188 RAYFYGYESNLGRSLRSSDFKSARDTDGHGTHTASTAAGSFVKNASFFQYARGEARGMAS 247
           R +  GYES +G    S + +S RD DGHGTHT+STAAGS V+ AS   YA G ARGMA 
Sbjct: 187 RFFARGYESTMGPIDESKESRSPRDDDGHGTHTSSTAAGSVVEGASLLGYASGTARGMAP 246

Query: 248 RARIAAYKICWEYGCFDSDILAAMDQAIADGVHIISLSVGSSGRAPAYYRDSIAIGAFGA 307
           RAR+A YK+CW  GCF SDILAA+D+AIAD V+++S+S+G  G    YYRD +AIGAF A
Sbjct: 247 RARVAVYKVCWLGGCFSSDILAAIDKAIADNVNVLSMSLG--GGMSDYYRDGVAIGAFAA 306

Query: 308 MQHGVVVSCSAGNSGPGSYTAVNIAPWILTVGASTIDREFLADVILGDGRVFSGVSLYSG 367
           M+ G++VSCSAGN+GP S +  N+APWI TVGA T+DR+F A  ILG+G+ F+GVSL+ G
Sbjct: 307 MERGILVSCSAGNAGPSSSSLSNVAPWITTVGAGTLDRDFPALAILGNGKNFTGVSLFKG 366

Query: 368 EPLGDSKLPLVYGGDCGS----RYCYSGSLDSSKVAGKIVVCDRGGNARVAKGSAVKSAG 427
           E L D  LP +Y G+  +      C +G+L   KV GKIV+CDRG NARV KG  VK+AG
Sbjct: 367 EALPDKLLPFIYAGNASNATNGNLCMTGTLIPEKVKGKIVMCDRGINARVQKGDVVKAAG 426

Query: 428 GLGMVLANTEENGEELLADSHLIPGTMVGEIAGNKLRNYTHSDPNPTATIVFRGTVIGNS 487
           G+GM+LANT  NGEEL+AD+HL+P T VGE AG+ +R+Y  +DPNPTA+I   GTV+G  
Sbjct: 427 GVGMILANTAANGEELVADAHLLPATTVGEKAGDIIRHYVTTDPNPTASISILGTVVGVK 486

Query: 488 PSAPRVAAFSSRGPNYRTAEILKPDVIAPGVNILAGWSGYSSPTSLSIDPRRVEFNIISG 547
           PS P VAAFSSRGPN  T  ILKPD+IAPGVNILA W+G + PT L+ D RRVEFNIISG
Sbjct: 487 PS-PVVAAFSSRGPNSITPNILKPDLIAPGVNILAAWTGAAGPTGLASDSRRVEFNIISG 546

Query: 548 TSMSCPHVSGVAALLRKAFPTWSPAAIKSALITTSYSLDSSGSPIKDLSTSKESNPFVHG 607
           TSMSCPHVSG+AALL+   P WSPAAI+SAL+TT+Y     G P+ D++T K S PF HG
Sbjct: 547 TSMSCPHVSGLAALLKSVHPEWSPAAIRSALMTTAYKTYKDGKPLLDIATGKPSTPFDHG 606

Query: 608 AGHIDPNKALNPGLIYDLTPQDYVSFLCSIGYDSRQIAVFVKDSSYSQLCE-HKLSNPGN 667
           AGH+ P  A NPGLIYDLT +DY+ FLC++ Y S QI      S  +  C+  K  +  +
Sbjct: 607 AGHVSPTTATNPGLIYDLTTEDYLGFLCALNYTSPQIR---SVSRRNYTCDPSKSYSVAD 666

Query: 668 LNYPSFAVVFDDEEVVKYTRTVTNVGDETDAVYEVKVES-PQGVEISVVPNKLEFNEKKT 727
           LNYPSFAV  D     KYTRTVT+VG      Y VKV S   GV+ISV P  L F E   
Sbjct: 667 LNYPSFAVNVDGVGAYKYTRTVTSVGGA--GTYSVKVTSETTGVKISVEPAVLNFKEANE 726

Query: 728 TQSYEITFT----KISGFKELASFGSIQWSDGSHNVRSPIAVSF 761
            +SY +TFT    K SG     SFGSI+WSDG H V SP+A+S+
Sbjct: 727 KKSYTVTFTVDSSKPSGSN---SFGSIEWSDGKHVVGSPVAISW 756

BLAST of Lsi02G028920 vs. TAIR10
Match: AT2G05920.1 (AT2G05920.1 Subtilase family protein)

HSP 1 Score: 749.2 bits (1933), Expect = 2.5e-216
Identity = 395/738 (53.52%), Postives = 504/738 (68.29%), Query Frame = 1

Query: 30  QETFIVHVSKSEKPSLFSSHNHWHSSILESLSPSPHPTKLLYSYERAVNGFSASITAAQA 89
           ++T+I+ V+ S+KP  F +H+ W++S L S S       LLY+Y  + +GFSA + + +A
Sbjct: 27  KKTYIIRVNHSDKPESFLTHHDWYTSQLNSES------SLLYTYTTSFHGFSAYLDSTEA 86

Query: 90  AELRHVPG-VISVIPDRIRQLHTTRTPHFLGLADNLGLWADTNYAEDVIIGVLDTGIWPE 149
             L      ++ +  D +  LHTTRTP FLGL    G+    + +  VIIGVLDTG+WPE
Sbjct: 87  DSLLSSSNSILDIFEDPLYTLHTTRTPEFLGLNSEFGVHDLGSSSNGVIIGVLDTGVWPE 146

Query: 150 RPSFSDEGLSPVPASWKGTCETGEGFSASACNRKIIGARAYFYGYESNLGRSLRSS-DFK 209
             SF D  +  +P+ WKG CE+G  F +  CN+K+IGAR++  G++   G    S  +  
Sbjct: 147 SRSFDDTDMPEIPSKWKGECESGSDFDSKLCNKKLIGARSFSKGFQMASGGGFSSKRESV 206

Query: 210 SARDTDGHGTHTASTAAGSFVKNASFFQYARGEARGMASRARIAAYKICWEYGCFDSDIL 269
           S RD DGHGTHT++TAAGS V+NASF  YA G ARGMA+RAR+A YK+CW  GCF SDIL
Sbjct: 207 SPRDVDGHGTHTSTTAAGSAVRNASFLGYAAGTARGMATRARVATYKVCWSTGCFGSDIL 266

Query: 270 AAMDQAIADGVHIISLSVGSSGRAPAYYRDSIAIGAFGAMQHGVVVSCSAGNSGPGSYTA 329
           AAMD+AI DGV ++SLS+G  G AP YYRD+IAIGAF AM+ GV VSCSAGNSGP   + 
Sbjct: 267 AAMDRAILDGVDVLSLSLGG-GSAP-YYRDTIAIGAFSAMERGVFVSCSAGNSGPTRASV 326

Query: 330 VNIAPWILTVGASTIDREFLADVILGDGRVFSGVSLYSGEPLGDSKLPLVY--GGDCGSR 389
            N+APW++TVGA T+DR+F A   LG+G+  +GVSLYSG  +G   L LVY  G    S 
Sbjct: 327 ANVAPWVMTVGAGTLDRDFPAFANLGNGKRLTGVSLYSGVGMGTKPLELVYNKGNSSSSN 386

Query: 390 YCYSGSLDSSKVAGKIVVCDRGGNARVAKGSAVKSAGGLGMVLANTEENGEELLADSHLI 449
            C  GSLDSS V GKIVVCDRG NARV KG+ V+ AGGLGM++ANT  +GEEL+ADSHL+
Sbjct: 387 LCLPGSLDSSIVRGKIVVCDRGVNARVEKGAVVRDAGGLGMIMANTAASGEELVADSHLL 446

Query: 450 PGTMVGEIAGNKLRNYTHSDPNPTATIVFRGTVIGNSPSAPRVAAFSSRGPNYRTAEILK 509
           P   VG+  G+ LR Y  SD  PTA +VF+GTV+   PS P VAAFSSRGPN  T EILK
Sbjct: 447 PAIAVGKKTGDLLREYVKSDSKPTALLVFKGTVLDVKPS-PVVAAFSSRGPNTVTPEILK 506

Query: 510 PDVIAPGVNILAGWSGYSSPTSLSIDPRRVEFNIISGTSMSCPHVSGVAALLRKAFPTWS 569
           PDVI PGVNILAGWS    PT L  D RR +FNI+SGTSMSCPH+SG+A LL+ A P WS
Sbjct: 507 PDVIGPGVNILAGWSDAIGPTGLDKDSRRTQFNIMSGTSMSCPHISGLAGLLKAAHPEWS 566

Query: 570 PAAIKSALITTSYSLDSSGSPIKDLSTSKESNPFVHGAGHIDPNKALNPGLIYDLTPQDY 629
           P+AIKSAL+TT+Y LD++ +P+ D + +  SNP+ HG+GH+DP KAL+PGL+YD++ ++Y
Sbjct: 567 PSAIKSALMTTAYVLDNTNAPLHDAADNSLSNPYAHGSGHVDPQKALSPGLVYDISTEEY 626

Query: 630 VSFLCSIGYDSRQIAVFVKDSSYSQLCEHKLSNPGNLNYPSFAVVFDDEEVVKYTRTVTN 689
           + FLCS+ Y    I   VK  S +  C  K S+PG LNYPSF+V+F  + VV+YTR VTN
Sbjct: 627 IRFLCSLDYTVDHIVAIVKRPSVN--CSKKFSDPGQLNYPSFSVLFGGKRVVRYTREVTN 686

Query: 690 VGDETDAVYEVKVESPQGVEISVVPNKLEFNEKKTTQSYEITFTKISG--FKELASFGSI 749
           VG    +VY+V V     V ISV P+KL F      + Y +TF    G      A FGSI
Sbjct: 687 VG-AASSVYKVTVNGAPSVGISVKPSKLSFKSVGEKKRYTVTFVSKKGVSMTNKAEFGSI 746

Query: 750 QWSDGSHNVRSPIAVSFN 762
            WS+  H VRSP+A S+N
Sbjct: 747 TWSNPQHEVRSPVAFSWN 752

BLAST of Lsi02G028920 vs. TAIR10
Match: AT3G14240.1 (AT3G14240.1 Subtilase family protein)

HSP 1 Score: 738.0 bits (1904), Expect = 5.8e-213
Identity = 391/774 (50.52%), Postives = 519/774 (67.05%), Query Frame = 1

Query: 11  FFFFILCFVPVIFSRSPDNQETFIVHVSKSEKPSLFSSHNHWHSSILESLSPSPHPTKLL 70
           +FFF+L       S S  N  T+IVHV    KPS+F +H HW++S L SL+ SP    ++
Sbjct: 6   YFFFLLTLSSPSSSASSSNSLTYIVHVDHEAKPSIFPTHFHWYTSSLASLTSSP--PSII 65

Query: 71  YSYERAVNGFSASITAAQAAELRHVPGVISVIPDRIRQLHTTRTPHFLGL--ADNLGLWA 130
           ++Y+   +GFSA +T+  A++L   P VISVIP+++R LHTTR+P FLGL   D  GL  
Sbjct: 66  HTYDTVFHGFSARLTSQDASQLLDHPHVISVIPEQVRHLHTTRSPEFLGLRSTDKAGLLE 125

Query: 131 DTNYAEDVIIGVLDTGIWPERPSFSDEGLSPVPASWKGTCETGEGFSASACNRKIIGARA 190
           ++++  D++IGV+DTG+WPERPSF D GL PVP  WKG C   + F  SACNRK++GAR 
Sbjct: 126 ESDFGSDLVIGVIDTGVWPERPSFDDRGLGPVPIKWKGQCIASQDFPESACNRKLVGARF 185

Query: 191 YFYGYESNLGRSLRSSDFKSARDTDGHGTHTASTAAGSFVKNASFFQYARGEARGMASRA 250
           +  GYE+  G+   +++F+S RD+DGHGTHTAS +AG +V  AS   YA G A GMA +A
Sbjct: 186 FCGGYEATNGKMNETTEFRSPRDSDGHGTHTASISAGRYVFPASTLGYAHGVAAGMAPKA 245

Query: 251 RIAAYKICWEYGCFDSDILAAMDQAIADGVHIISLSVGSSGRAPAYYRDSIAIGAFGAMQ 310
           R+AAYK+CW  GC+DSDILAA D A+ADGV +ISLSVG  G    YY D+IAIGAFGA+ 
Sbjct: 246 RLAAYKVCWNSGCYDSDILAAFDTAVADGVDVISLSVG--GVVVPYYLDAIAIGAFGAID 305

Query: 311 HGVVVSCSAGNSGPGSYTAVNIAPWILTVGASTIDREFLADVILGDGRVFSGVSLYSGEP 370
            G+ VS SAGN GPG+ T  N+APW+ TVGA TIDR+F A+V LG+G++ SGVS+Y G  
Sbjct: 306 RGIFVSASAGNGGPGALTVTNVAPWMTTVGAGTIDRDFPANVKLGNGKMISGVSVYGGPG 365

Query: 371 LGDSKL-PLVYGGDC------GSRYCYSGSLDSSKVAGKIVVCDRGGNARVAKGSAVKSA 430
           L   ++ PLVYGG         S  C  GSLD + V GKIV+CDRG N+R  KG  V+  
Sbjct: 366 LDPGRMYPLVYGGSLLGGDGYSSSLCLEGSLDPNLVKGKIVLCDRGINSRATKGEIVRKN 425

Query: 431 GGLGMVLANTEENGEELLADSHLIPGTMVGEIAGNKLRNY------THSDPNPTATIVFR 490
           GGLGM++AN   +GE L+AD H++P T VG   G+++R Y      + S  +PTATIVF+
Sbjct: 426 GGLGMIIANGVFDGEGLVADCHVLPATSVGASGGDEIRRYISESSKSRSSKHPTATIVFK 485

Query: 491 GTVIGNSPSAPRVAAFSSRGPNYRTAEILKPDVIAPGVNILAGWSGYSSPTSLSIDPRRV 550
           GT +G  P AP VA+FS+RGPN  T EILKPDVIAPG+NILA W     P+ ++ D RR 
Sbjct: 486 GTRLGIRP-APVVASFSARGPNPETPEILKPDVIAPGLNILAAWPDRIGPSGVTSDNRRT 545

Query: 551 EFNIISGTSMSCPHVSGVAALLRKAFPTWSPAAIKSALITTSYSLDSSGSPIKDLSTSKE 610
           EFNI+SGTSM+CPHVSG+AALL+ A P WSPAAI+SALITT+Y++D+SG P+ D ST   
Sbjct: 546 EFNILSGTSMACPHVSGLAALLKAAHPDWSPAAIRSALITTAYTVDNSGEPMMDESTGNT 605

Query: 611 SNPFVHGAGHIDPNKALNPGLIYDLTPQDYVSFLCSIGYDSRQIAVFVKDSSYSQLCE-- 670
           S+   +G+GH+ P KA++PGL+YD+T  DY++FLC+  Y    I    +  +    C+  
Sbjct: 606 SSVMDYGSGHVHPTKAMDPGLVYDITSYDYINFLCNSNYTRTNIVTITRRQAD---CDGA 665

Query: 671 HKLSNPGNLNYPSFAVVF----DDEEVVKYTRTVTNVGDETDAVYEVKVESPQGVEISVV 730
            +  + GNLNYPSF+VVF    + +    + RTVTNVGD +D+VYE+K+  P+G  ++V 
Sbjct: 666 RRAGHVGNLNYPSFSVVFQQYGESKMSTHFIRTVTNVGD-SDSVYEIKIRPPRGTTVTVE 725

Query: 731 PNKLEFNEKKTTQSYEI----TFTKISGFKELASFGSIQWSDGSHNVRSPIAVS 760
           P KL F       S+ +    T  K+S        G I WSDG  NV SP+ V+
Sbjct: 726 PEKLSFRRVGQKLSFVVRVKTTEVKLSPGATNVETGHIVWSDGKRNVTSPLVVT 770

BLAST of Lsi02G028920 vs. TAIR10
Match: AT5G51750.1 (AT5G51750.1 subtilase 1.3)

HSP 1 Score: 715.3 bits (1845), Expect = 4.0e-206
Identity = 380/773 (49.16%), Postives = 512/773 (66.24%), Query Frame = 1

Query: 12  FFFILCFVPVIFSRSP-----DNQETFIVHVSKSEKPSLFSSHNHWHSSILESLSPSP-- 71
           F FI+  + +IF ++        ++T+++H+ KS  P  +++H  W+SS + S++     
Sbjct: 11  FLFIILSINLIFLQAETTTQISTKKTYVIHMDKSAMPLPYTNHLQWYSSKINSVTQHKSQ 70

Query: 72  ----HPTKLLYSYERAVNGFSASITAAQAAELRHVPGVISVIPDRIRQLHTTRTPHFLGL 131
               +  ++LY+Y+ A +G +A +T  +A  L    GV++VIP+   +LHTTR+P FLGL
Sbjct: 71  EEEGNNNRILYTYQTAFHGLAAQLTQEEAERLEEEDGVVAVIPETRYELHTTRSPTFLGL 130

Query: 132 A--DNLGLWADTNYAEDVIIGVLDTGIWPERPSFSDEGLSPVPASWKGTCETGEGFSASA 191
              ++  +WA+     DV++GVLDTGIWPE  SF+D G+SPVPA+W+G CETG+ F    
Sbjct: 131 ERQESERVWAERVTDHDVVVGVLDTGIWPESESFNDTGMSPVPATWRGACETGKRFLKRN 190

Query: 192 CNRKIIGARAYFYGYESNLGRSLRSSDFKSARDTDGHGTHTASTAAGSFVKNASFFQYAR 251
           CNRKI+GAR ++ GYE+  G+     ++KS RD DGHGTHTA+T AGS VK A+ F +A 
Sbjct: 191 CNRKIVGARVFYRGYEAATGKIDEELEYKSPRDRDGHGTHTAATVAGSPVKGANLFGFAY 250

Query: 252 GEARGMASRARIAAYKICWEYGCFDSDILAAMDQAIADGVHIISLSVGSSGRAPAYYRDS 311
           G ARGMA +AR+AAYK+CW  GCF SDIL+A+DQA+ADGV ++S+S+G  G    Y RDS
Sbjct: 251 GTARGMAQKARVAAYKVCWVGGCFSSDILSAVDQAVADGVQVLSISLG--GGVSTYSRDS 310

Query: 312 IAIGAFGAMQHGVVVSCSAGNSGPGSYTAVNIAPWILTVGASTIDREFLADVILGDGRVF 371
           ++I  FGAM+ GV VSCSAGN GP   +  N++PWI TVGAST+DR+F A V +G  R F
Sbjct: 311 LSIATFGAMEMGVFVSCSAGNGGPDPISLTNVSPWITTVGASTMDRDFPATVKIGTMRTF 370

Query: 372 SGVSLYSGEPL--GDSKLPLVYGGDCGS-----RYCYSGSLDSSKVAGKIVVCDRGGNAR 431
            GVSLY G  +   + + PLVY G   S      +C  G+LD   VAGKIV+CDRG   R
Sbjct: 371 KGVSLYKGRTVLPKNKQYPLVYLGRNASSPDPTSFCLDGALDRRHVAGKIVICDRGVTPR 430

Query: 432 VAKGSAVKSAGGLGMVLANTEENGEELLADSHLIPGTMVGEIAGNKLRNYTHSDPNPTAT 491
           V KG  VK AGG+GMVL NT  NGEEL+ADSH++P   VGE  G  ++ Y  +    TA+
Sbjct: 431 VQKGQVVKRAGGIGMVLTNTATNGEELVADSHMLPAVAVGEKEGKLIKQYAMTSKKATAS 490

Query: 492 IVFRGTVIGNSPSAPRVAAFSSRGPNYRTAEILKPDVIAPGVNILAGWSGYSSPTSLSID 551
           +   GT IG  PS P VAAFSSRGPN+ + EILKPD++APGVNILA W+G  +P+SLS D
Sbjct: 491 LEILGTRIGIKPS-PVVAAFSSRGPNFLSLEILKPDLLAPGVNILAAWTGDMAPSSLSSD 550

Query: 552 PRRVEFNIISGTSMSCPHVSGVAALLRKAFPTWSPAAIKSALITTSYSLDSSGSPIKDLS 611
           PRRV+FNI+SGTSMSCPHVSGVAAL++   P WSPAAIKSAL+TT+Y  D+   P+ D S
Sbjct: 551 PRRVKFNILSGTSMSCPHVSGVAALIKSRHPDWSPAAIKSALMTTAYVHDNMFKPLTDAS 610

Query: 612 TSKESNPFVHGAGHIDPNKALNPGLIYDLTPQDYVSFLCSIGYDSRQIAVFVKDSSYSQL 671
            +  S+P+ HGAGHIDP +A +PGL+YD+ PQ+Y  FLC+      Q+ VF K S  ++ 
Sbjct: 611 GAAPSSPYDHGAGHIDPLRATDPGLVYDIGPQEYFEFLCTQDLSPSQLKVFTKHS--NRT 670

Query: 672 CEHKLS-NPGNLNYPSFAVVFDDEEVVK---YTRTVTNVGDETDAVYEVKVESPQGVEIS 731
           C+H L+ NPGNLNYP+ + +F +   VK     RTVTNVG    + Y+V V   +G  ++
Sbjct: 671 CKHTLAKNPGNLNYPAISALFPENTHVKAMTLRRTVTNVGPHISS-YKVSVSPFKGASVT 730

Query: 732 VVPNKLEFNEKKTTQSYEITFTKISGFKELASFGSIQWSDGSHNVRSPIAVSF 761
           V P  L F  K    SY +TF      K    FG + W   +H VRSP+ +++
Sbjct: 731 VQPKTLNFTSKHQKLSYTVTFRTRFRMKR-PEFGGLVWKSTTHKVRSPVIITW 776

BLAST of Lsi02G028920 vs. NCBI nr
Match: gi|659124686|ref|XP_008462294.1| (PREDICTED: LOW QUALITY PROTEIN: subtilisin-like protease [Cucumis melo])

HSP 1 Score: 1393.3 bits (3605), Expect = 0.0e+00
Identity = 699/770 (90.78%), Postives = 736/770 (95.58%), Query Frame = 1

Query: 1   MANFHLCSSLFFFFILCFV-PVIFSRSPDNQETFIVHVSKSEKPSLFSSHNHWHSSILES 60
           MANFHL SS FFFF + FV P++FSRS + QET+IVHVSKSEKPSLFSSH+HWHSSILES
Sbjct: 1   MANFHLPSSSFFFFFVLFVFPLVFSRSLETQETYIVHVSKSEKPSLFSSHHHWHSSILES 60

Query: 61  LSPSPHPTKLLYSYERAVNGFSASITAAQAAELRHVPGVISVIPDRIRQLHTTRTPHFLG 120
           LSPSPHPTKLLY+YERA NGFSA IT AQA ELR VPG+ISVIPDRIRQLHTTRTPHFLG
Sbjct: 61  LSPSPHPTKLLYNYERAANGFSARITTAQAEELRRVPGIISVIPDRIRQLHTTRTPHFLG 120

Query: 121 LADNLGLWADTNYAEDVIIGVLDTGIWPERPSFSDEGLSPVPASWKGTCETGEGFSASAC 180
           LADNLGLWADTNYA+DVIIGVLDTGIWPERPSFSDEGL+PVPA WKGTC+TGEG SA AC
Sbjct: 121 LADNLGLWADTNYADDVIIGVLDTGIWPERPSFSDEGLAPVPARWKGTCDTGEGSSALAC 180

Query: 181 NRKIIGARAYFYGYESNLGRSLR-SSDFKSARDTDGHGTHTASTAAGSFVKNASFFQYAR 240
           NRKIIGARA+F GYESNL  SL+ SSDFKSARDTDGHGTHTASTAAGSFVKNASFFQYAR
Sbjct: 181 NRKIIGARAFFXGYESNLRGSLKGSSDFKSARDTDGHGTHTASTAAGSFVKNASFFQYAR 240

Query: 241 GEARGMASRARIAAYKICWEYGCFDSDILAAMDQAIADGVHIISLSVGSSGRAPAYYRDS 300
           GEARGMASRARIAAYKICWE+GC+DSDILAAMDQAI+DGV +ISLSVGSSGRAPAYYRDS
Sbjct: 241 GEARGMASRARIAAYKICWEFGCYDSDILAAMDQAISDGVDVISLSVGSSGRAPAYYRDS 300

Query: 301 IAIGAFGAMQHGVVVSCSAGNSGPGSYTAVNIAPWILTVGASTIDREFLADVILGDGRVF 360
           IAIGAFGAMQHG+VVSCSAGNSGPG YTAVNIAPWI+TVGASTIDREFLADVILGD RVF
Sbjct: 301 IAIGAFGAMQHGIVVSCSAGNSGPGPYTAVNIAPWIVTVGASTIDREFLADVILGDNRVF 360

Query: 361 SGVSLYSGEPLGDSKLPLVYGGDCGSRYCYSGSLDSSKVAGKIVVCDRGGNARVAKGSAV 420
           SGVSLYSG+PLGDSKLPLVYGGDCGSRYCYSGSLDSSKVAG+IVVCDRGGNARVAKG AV
Sbjct: 361 SGVSLYSGDPLGDSKLPLVYGGDCGSRYCYSGSLDSSKVAGRIVVCDRGGNARVAKGGAV 420

Query: 421 KSAGGLGMVLANTEENGEELLADSHLIPGTMVGEIAGNKLRNYTHSDPNPTATIVFRGTV 480
           KSAGGLGMVLANTEENGEELLADSHLIPGTMVGEIAGNKLR+Y H+DPNPTATIVFRGTV
Sbjct: 421 KSAGGLGMVLANTEENGEELLADSHLIPGTMVGEIAGNKLRDYIHTDPNPTATIVFRGTV 480

Query: 481 IGNSPSAPRVAAFSSRGPNYRTAEILKPDVIAPGVNILAGWSGYSSPTSLSIDPRRVEFN 540
           IG+SP AP+VA+FSSRGPNYRTAEILKPDVIAPGVNILAGWSGYSSPT L+IDPRRVEFN
Sbjct: 481 IGDSPPAPKVASFSSRGPNYRTAEILKPDVIAPGVNILAGWSGYSSPTGLNIDPRRVEFN 540

Query: 541 IISGTSMSCPHVSGVAALLRKAFPTWSPAAIKSALITTSYSLDSSGSPIKDLSTSKESNP 600
           IISGTSMSCPHVSGVAALLRKAFPTWSPAAIKSALITTSYSLDSSGSPIKDL+TS+ESNP
Sbjct: 541 IISGTSMSCPHVSGVAALLRKAFPTWSPAAIKSALITTSYSLDSSGSPIKDLATSEESNP 600

Query: 601 FVHGAGHIDPNKALNPGLIYDLTPQDYVSFLCSIGYDSRQIAVFVKDSSYSQLCEHKLSN 660
           FVHGAGHIDPN+ALNPGLIYDLTPQDYVSFLCSIGYDS+QIAVFVK SSYSQLCEHKLSN
Sbjct: 601 FVHGAGHIDPNQALNPGLIYDLTPQDYVSFLCSIGYDSKQIAVFVKGSSYSQLCEHKLSN 660

Query: 661 PGNLNYPSFAVVFDDEEVVKYTRTVTNVGDETDAVYEVKVESPQGVEISVVPNKLEFNEK 720
           PGNLNYPSF+VVFDD EVVKYTRTVTNVGDET+ VY VKVE+PQGVEISVVPNKLEFN++
Sbjct: 661 PGNLNYPSFSVVFDDGEVVKYTRTVTNVGDETEVVYGVKVEAPQGVEISVVPNKLEFNKE 720

Query: 721 KTTQSYEITFTKISGFKELASFGSIQWSDGSHNVRSPIAVSFNTGSIASM 769
           KTT SYEITFTKI+GFKE ASFGSIQWSDG HNVRSPIAVSF TGSIASM
Sbjct: 721 KTTLSYEITFTKINGFKESASFGSIQWSDGIHNVRSPIAVSFKTGSIASM 770

BLAST of Lsi02G028920 vs. NCBI nr
Match: gi|449447904|ref|XP_004141706.1| (PREDICTED: subtilisin-like protease SBT1.7 [Cucumis sativus])

HSP 1 Score: 1388.2 bits (3592), Expect = 0.0e+00
Identity = 699/771 (90.66%), Postives = 733/771 (95.07%), Query Frame = 1

Query: 1   MANFHL-CSSLFFFFILCFVPVIFSRSPDNQETFIVHVSKSEKPSLFSSHNHWHSSILES 60
           MANFHL  SS FFFF+ CF P+IFSRS +NQET+IVHVSKSEKPSLFSSH+HWHSSILES
Sbjct: 1   MANFHLPSSSFFFFFLFCFFPLIFSRSLENQETYIVHVSKSEKPSLFSSHHHWHSSILES 60

Query: 61  LSPSPHPTKLLYSYERAVNGFSASITAAQAAELRHVPGVISVIPDRIRQLHTTRTPHFLG 120
           LS SPHPTKLLY+YERA NGFSA IT  QA ELR VPG+ISVIPD+IRQLHTTRTPHFLG
Sbjct: 61  LSSSPHPTKLLYNYERAANGFSARITTVQAEELRRVPGIISVIPDQIRQLHTTRTPHFLG 120

Query: 121 LADNLGLWADTNYAEDVIIGVLDTGIWPERPSFSDEGLSPVPASWKGTCETGEGFSASAC 180
           LADNLGLWADTNYA+DVIIGVLDTGIWPERPSFSDEGLSPVPA WKGTC+TGEG SA AC
Sbjct: 121 LADNLGLWADTNYADDVIIGVLDTGIWPERPSFSDEGLSPVPARWKGTCDTGEGVSAFAC 180

Query: 181 NRKIIGARAYFYGYESNLGRSLR-SSDFKSARDTDGHGTHTASTAAGSFVKNASFFQYAR 240
           NRKIIGARAYFYGYESNL  SL+ SSDFKSARDT+GHGTHTASTAAGSFV NASFFQYAR
Sbjct: 181 NRKIIGARAYFYGYESNLRGSLKVSSDFKSARDTEGHGTHTASTAAGSFVNNASFFQYAR 240

Query: 241 GEARGMASRARIAAYKICWEYGCFDSDILAAMDQAIADGVHIISLSVGSSGRAPAYYRDS 300
           GEARGMASRARIAAYKICWE+GC+DSDILAAMDQAI+DGV +ISLSVGSSGRAPAYYRDS
Sbjct: 241 GEARGMASRARIAAYKICWEFGCYDSDILAAMDQAISDGVDVISLSVGSSGRAPAYYRDS 300

Query: 301 IAIGAFGAMQHGVVVSCSAGNSGPGSYTAVNIAPWILTVGASTIDREFLADVILGDGRVF 360
           IAIGAFGAMQHGVVVSCSAGNSGPG YTAVNIAPWILTVGASTIDREFLADVILGDGRVF
Sbjct: 301 IAIGAFGAMQHGVVVSCSAGNSGPGPYTAVNIAPWILTVGASTIDREFLADVILGDGRVF 360

Query: 361 SGVSLYSGEPLGDSKLPLVYGGDCGSRYCYSGSLDSSKVAGKIVVCDRGGNARVAKGSAV 420
           SGVSLYSG+PLGDSKL LVYGGDCGSRYCYSGSLDSSKVAGKIVVCDRGGNARVAKG AV
Sbjct: 361 SGVSLYSGDPLGDSKLQLVYGGDCGSRYCYSGSLDSSKVAGKIVVCDRGGNARVAKGGAV 420

Query: 421 KSAGGLGMVLANTEENGEELLADSHLIPGTMVGEIAGNKLRNYTHSDPNPTATIVFRGTV 480
           KSAGGLGMVLANTEENGEELLADSHLIPGTMVG IAGNKLR+Y H+DPNPTATIVFRGTV
Sbjct: 421 KSAGGLGMVLANTEENGEELLADSHLIPGTMVGAIAGNKLRDYIHTDPNPTATIVFRGTV 480

Query: 481 IGNSPSAPRVAAFSSRGPNYRTAEILKPDVIAPGVNILAGWSGYSSPTSLSIDPRRVEFN 540
           IG+SP APRVAAFSSRGPNYRTAEILKPDVIAPGVNILAGWSGYSSPT L+IDPRRVEFN
Sbjct: 481 IGDSPPAPRVAAFSSRGPNYRTAEILKPDVIAPGVNILAGWSGYSSPTGLNIDPRRVEFN 540

Query: 541 IISGTSMSCPHVSGVAALLRKAFPTWSPAAIKSALITTSYSLDSSGSPIKDLSTSKESNP 600
           IISGTSMSCPHVSGVAALLRKAFPTWSPAAIKSALITTSYSLDSSG PIKDLSTS+ESNP
Sbjct: 541 IISGTSMSCPHVSGVAALLRKAFPTWSPAAIKSALITTSYSLDSSGKPIKDLSTSEESNP 600

Query: 601 FVHGAGHIDPNKALNPGLIYDLTPQDYVSFLCSIGYDSRQIAVFVKDSSYSQLCEHKLSN 660
           FVHGAGHI+PN+ALNPGLIYDLTPQDYVSFLCSIGYDS+QIAVFVK SSY QLCEHKL+N
Sbjct: 601 FVHGAGHINPNQALNPGLIYDLTPQDYVSFLCSIGYDSKQIAVFVKGSSYFQLCEHKLTN 660

Query: 661 PGNLNYPSFAVVFDDEEVVKYTRTVTNVGDETDAVYEVKVESPQGVEISVVPNKLEFNEK 720
           PGNLNYPSF+VVFD+EEVVKYTRTVTNVGDET+ VYEVKVE+PQGV ISVVPNKLEFN++
Sbjct: 661 PGNLNYPSFSVVFDEEEVVKYTRTVTNVGDETEVVYEVKVEAPQGVVISVVPNKLEFNKE 720

Query: 721 KTTQSYEITFTKISGFKELASFGSIQWSDGSHNVRSPIAVSFNT-GSIASM 769
           KTTQSYEITFTKI+GFKE ASFGSIQW DG H+VRSPIAVSF T GSIASM
Sbjct: 721 KTTQSYEITFTKINGFKESASFGSIQWGDGIHSVRSPIAVSFKTGGSIASM 771

BLAST of Lsi02G028920 vs. NCBI nr
Match: gi|470140051|ref|XP_004305758.1| (PREDICTED: subtilisin-like protease [Fragaria vesca subsp. vesca])

HSP 1 Score: 1079.3 bits (2790), Expect = 0.0e+00
Identity = 547/767 (71.32%), Postives = 636/767 (82.92%), Query Frame = 1

Query: 9   SLFFFFILCFVPVIFSRSP---DNQETFIVHVSKSEKPSLFSSHNHWHSSILESLSPSPH 68
           SLFFF +LCFV   FS      D+  TFIVHVSK  KP+LFSS   W++SIL SL PSPH
Sbjct: 7   SLFFFLVLCFVHATFSTDQSDRDSPRTFIVHVSKYSKPALFSSQRRWYTSILRSLPPSPH 66

Query: 69  PTKLLYSYERAVNGFSASITAAQAAELRHVPGVISVIPDRIRQLHTTRTPHFLGLADNLG 128
           PTKLLY+Y RAV+GFSA+++A+QA  L+  P V+SV+PD  RQLHTTRT  FLGLADN G
Sbjct: 67  PTKLLYTYSRAVHGFSATLSASQAHALQSHPAVLSVVPDMPRQLHTTRTYDFLGLADNFG 126

Query: 129 LWADTNYAEDVIIGVLDTGIWPERPSFSDEGLSPVPASWKGTCETGEGFSASACNRKIIG 188
           +W +++YA+DVIIGVLDTGIWPERPSFSD GL PVP +WKG C     F AS+CNRKIIG
Sbjct: 127 IWPNSDYADDVIIGVLDTGIWPERPSFSDSGLGPVPKTWKGKCVITGDFPASSCNRKIIG 186

Query: 189 ARAYFYGYESNLGRSL-RSSDFKSARDTDGHGTHTASTAAGSFVKNASFFQYARGEARGM 248
           ARAYF GYES+LG+ +  S++ +S RDT+GHGTHTASTA GS V NASF++YA GEARGM
Sbjct: 187 ARAYFNGYESHLGKPMDESNESRSPRDTEGHGTHTASTAGGSRVSNASFYEYASGEARGM 246

Query: 249 ASRARIAAYKICWEYGCFDSDILAAMDQAIADGVHIISLSVGSSGRAPAYYRDSIAIGAF 308
           AS+ARIAAYKICW +GCFDSDILAAMDQAIADGVHIISLSVG+SG AP Y RDSIAIGAF
Sbjct: 247 ASKARIAAYKICWTFGCFDSDILAAMDQAIADGVHIISLSVGASGGAPPYDRDSIAIGAF 306

Query: 309 GAMQHGVVVSCSAGNSGPGSYTAVNIAPWILTVGASTIDREFLADVILGDGRVFSGVSLY 368
           GA QHGV+VS SAGNSGPG +TA NIAPWILTVGAST+DREF ADV+LGDGRVF+GVSLY
Sbjct: 307 GAAQHGVLVSASAGNSGPGKFTATNIAPWILTVGASTLDREFPADVVLGDGRVFNGVSLY 366

Query: 369 SGEPLGDSKLPLVYGGDCGSRYCYSGSLDSSKVAGKIVVCDRGGNARVAKGSAVKSAGGL 428
           SGE L D KLPLVYGGDCGSR CYSG+L  SKV GKIVVCDRGGNARVAKGSAVK AGG+
Sbjct: 367 SGEGLMDYKLPLVYGGDCGSRLCYSGALQPSKVQGKIVVCDRGGNARVAKGSAVKLAGGI 426

Query: 429 GMVLANTEENGEELLADSHLIPGTMVGEIAGNKLRNYTHSDPNPTATIVFRGTVIGNSPS 488
           GM++ANTEE+GEELLADSHLIP TMVG++A +++R+Y  +  N TATI FRGTVIG SP 
Sbjct: 427 GMIMANTEESGEELLADSHLIPATMVGQMAADQIRSYIKTGHNATATIKFRGTVIGTSPP 486

Query: 489 APRVAAFSSRGPNYRTAEILKPDVIAPGVNILAGWSGYSSPTSLSIDPRRVEFNIISGTS 548
           +P+VA+FSSRGPN  T EILKPDVIAPGVNILAGW+G SSPT L IDPRRVEFNIISGTS
Sbjct: 487 SPKVASFSSRGPNSLTPEILKPDVIAPGVNILAGWTGASSPTDLDIDPRRVEFNIISGTS 546

Query: 549 MSCPHVSGVAALLRKAFPTWSPAAIKSALITTSYSLDSSGSPIKDLSTSKESNPFVHGAG 608
           MSCPHVSG+AALLRKA+P WSPAAIKSAL+TT+Y+LD+SG+ IKDL+   ES PFVHGAG
Sbjct: 547 MSCPHVSGIAALLRKAYPKWSPAAIKSALVTTAYTLDNSGNKIKDLANGGESTPFVHGAG 606

Query: 609 HIDPNKALNPGLIYDLTPQDYVSFLCSIGYDSRQIAVFVKDSSYSQLC-EHKLSNPGNLN 668
           H+DPN+ALNPGL+YD+   DYV+F+CSIGY  RQIAVF+++ +   +C  + L++PG+LN
Sbjct: 607 HVDPNRALNPGLVYDIDVNDYVAFMCSIGYGPRQIAVFMRELAGDDICARNSLASPGDLN 666

Query: 669 YPSFAVVF-DDEEVVKYTRTVTNVGDETDAVYEVKVESPQGVEISVVPNKLEFNEKKTTQ 728
           YPSFAVVF    E+VKY R VTNVG   DAVYEV V++P GVEISV P+KL F+E   TQ
Sbjct: 667 YPSFAVVFKPGRELVKYKRVVTNVGSVVDAVYEVNVDAPAGVEISVEPSKLVFSEVNQTQ 726

Query: 729 SYEITFTKISGFKELASFGSIQWSDGSHNVRSPIAVSF-NTGSIASM 769
           SYE+TF K  G+     +GSI+WSDG H+VRSP+AV + NTG  ASM
Sbjct: 727 SYEVTFAKGIGYVNGERYGSIEWSDGRHHVRSPVAVRWSNTGYSASM 773

BLAST of Lsi02G028920 vs. NCBI nr
Match: gi|565382138|ref|XP_006357406.1| (PREDICTED: subtilisin-like protease SBT1.7 [Solanum tuberosum])

HSP 1 Score: 1068.5 bits (2762), Expect = 5.3e-309
Identity = 537/778 (69.02%), Postives = 631/778 (81.11%), Query Frame = 1

Query: 1   MANFHLCSSLFFFFILCFVPVIFSRSPDNQETFIVHVSKSEKPSLFSSHNHWHSSILESL 60
           MA   + SS+FF    C  PV  S   D  ETFI+HV+KS+KP +FS+H+HW+SSI+ S+
Sbjct: 1   MAKISVLSSIFFIISFCLTPVAISVQSDGHETFIIHVAKSDKPHVFSTHHHWYSSIVRSI 60

Query: 61  SP-SPHPTKLLYSYERAVNGFSASITAAQAAELRHVPGVISVIPDRIRQLHTTRTPHFLG 120
           SP S H +K+LY+YERA  GFSA +TA QA +LR VPGVISVIPD++R LHTT TP FL 
Sbjct: 61  SPPSHHRSKILYTYERAAVGFSARLTAGQADQLRRVPGVISVIPDQVRYLHTTHTPTFLK 120

Query: 121 LADNLGLWADTNYAEDVIIGVLDTGIWPERPSFSDEGLSPVPASWKGTCETGEGFSASAC 180
           LAD+ GLW D++YA+DVI+GVLDTGIWPERPSFSDEGLSPVPA WKG C TG GF  S+C
Sbjct: 121 LADSFGLWPDSDYADDVIVGVLDTGIWPERPSFSDEGLSPVPAGWKGKCVTGPGFPRSSC 180

Query: 181 NRKIIGARAYFYGYESNLGRSLRSSDFKSARDTDGHGTHTASTAAGSFVKNASFFQYARG 240
           NRKIIGAR ++ GYE++ G    S + KS RDT+GHGTHTASTAAGS V NASF+QYA+G
Sbjct: 181 NRKIIGARMFYKGYEASHGPMDESKEAKSPRDTEGHGTHTASTAAGSLVANASFYQYAKG 240

Query: 241 EARGMASRARIAAYKICWEYGCFDSDILAAMDQAIADGVHIISLSVGSSGRAPAYYRDSI 300
           EARGMA +ARIAAYKICW+ GCFDSDILAAMDQA+ADGVH+ISLSVG++G AP Y  DSI
Sbjct: 241 EARGMAIKARIAAYKICWKDGCFDSDILAAMDQAVADGVHVISLSVGANGYAPHYLHDSI 300

Query: 301 AIGAFGAMQHGVVVSCSAGNSGPGSYTAVNIAPWILTVGASTIDREFLADVILGDGRVFS 360
           AIGAFGA +HGV+VSCSAGNSGPG YTAVNIAPWILTVGASTIDREF ADVILGD RVF 
Sbjct: 301 AIGAFGASEHGVLVSCSAGNSGPGPYTAVNIAPWILTVGASTIDREFPADVILGDDRVFG 360

Query: 361 GVSLYSGEPLGDSKLPLVYGGDCGSRYCYSGSLDSSKVAGKIVVCDRGGNARVAKGSAVK 420
           GVSLYSG PL DSK P+VY GDCGS+YCY G LD  KVAGKIV+CDRGGNARV KGSAVK
Sbjct: 361 GVSLYSGNPLTDSKFPVVYSGDCGSKYCYPGKLDHKKVAGKIVLCDRGGNARVEKGSAVK 420

Query: 421 SAGGLGMVLANTEENGEELLADSHLIPGTMVGEIAGNKLRNYTHSDPNPTATIVFRGTVI 480
            AGG+GM+LAN  E+GEEL+ADSHL+P TMVG+ AG+K+R Y  SD +PTATIVFRGTVI
Sbjct: 421 LAGGVGMILANLAESGEELVADSHLLPATMVGQKAGDKIREYVTSDTSPTATIVFRGTVI 480

Query: 481 GNSPSAPRVAAFSSRGPNYRTAEILKPDVIAPGVNILAGWSGYSSPTSLSIDPRRVEFNI 540
           GNSP+APRVAAFSSRGPN+ T EILKPDVIAPGVNILAGW+G + PT L+IDPRRVEFNI
Sbjct: 481 GNSPAAPRVAAFSSRGPNHLTPEILKPDVIAPGVNILAGWTGSTGPTDLAIDPRRVEFNI 540

Query: 541 ISGTSMSCPHVSGVAALLRKAFPTWSPAAIKSALITTSYSLDSSGSPIKDLSTSKESNPF 600
           ISGTSMSCPHVSG+AALLR+A   W+PAAIKSAL+TT+Y+LD+SG    DL+T +ES PF
Sbjct: 541 ISGTSMSCPHVSGLAALLRRAHSKWTPAAIKSALMTTAYNLDNSGKIFTDLATGEESTPF 600

Query: 601 VHGAGHIDPNKALNPGLIYDLTPQDYVSFLCSIGYDSRQIAVFVKDSSYSQLCEHKLSNP 660
           VHG+GH+DPN+AL+PGL+YD+   DYV+FLC+IGYD   IAVFV+DSS     E  L+ P
Sbjct: 601 VHGSGHVDPNRALDPGLVYDIETSDYVNFLCTIGYDGDDIAVFVRDSSRVNCSERSLATP 660

Query: 661 GNLNYPSFAVVF--DDEEVVKYTRTVTNVGDETDAVYEVKVESPQGVEISVVPNKLEFNE 720
           G+LNYPSF+V F  D   VVKY R V NVG +++AVYEVKV +P  VE+SV P KL F+E
Sbjct: 661 GDLNYPSFSVDFTSDSNGVVKYKRVVKNVGGDSNAVYEVKVNAPSAVEVSVSPAKLVFSE 720

Query: 721 KKTTQSYEITFTK-------ISGFKELASFGSIQWSDGSHNVRSPIAVSFNTGSIASM 769
           +  + SYEI+FT        + G +  ++FGSI+WSDG H+VRSPIAV +   S  SM
Sbjct: 721 ENNSLSYEISFTSKRSEDIMVKGIQ--SAFGSIEWSDGIHSVRSPIAVRWRYQSAVSM 776

BLAST of Lsi02G028920 vs. NCBI nr
Match: gi|590620870|ref|XP_007024651.1| (Subtilase family protein [Theobroma cacao])

HSP 1 Score: 1067.4 bits (2759), Expect = 1.2e-308
Identity = 541/760 (71.18%), Postives = 625/760 (82.24%), Query Frame = 1

Query: 9   SLFFFFILCFVPVIFSRSPDNQETFIVHVSKSEKPSLFSSHNHWHSSILESLSPSPHPTK 68
           S FFF  L F+P   S S D  E FI+HVSKS KPSLFSSH+HW+SSI+ SL PSPHPTK
Sbjct: 4   SFFFFLSLLFIP-FSSSSSDRPENFIIHVSKSHKPSLFSSHHHWYSSIIHSLPPSPHPTK 63

Query: 69  LLYSYERAVNGFSASITAAQAAELRHVPGVISVIPDRIRQLHTTRTPHFLGLADNLGLWA 128
           LLY+YERA+NGFSA +TA QA +LR +PG++SVIPD++RQ+HTTRTP FLGL+D +GLW 
Sbjct: 64  LLYTYERAINGFSARLTATQAEKLRELPGILSVIPDQVRQIHTTRTPQFLGLSDGVGLWQ 123

Query: 129 DTNYAEDVIIGVLDTGIWPERPSFSDEGLSPVPASWKGTCETGEGFSASACNRKIIGARA 188
           ++ Y + VIIGVLDTGIWPERPSF D GLSPVP SWKG CETG  F ASAC+RKIIGARA
Sbjct: 124 NSYYGDGVIIGVLDTGIWPERPSFKDSGLSPVPDSWKGICETGPDFPASACSRKIIGARA 183

Query: 189 YFYGYESNL-GRSLRSSDFKSARDTDGHGTHTASTAAGSFVKNASFFQYARGEARGMASR 248
           ++ GYES L G    + + KS RDT+GHGTHTASTAAGS V NAS F++A GEARGMA++
Sbjct: 184 FYKGYESYLEGPMDETKESKSPRDTEGHGTHTASTAAGSVVSNASLFEFAYGEARGMATK 243

Query: 249 ARIAAYKICWEYGCFDSDILAAMDQAIADGVHIISLSVGSSGRAPAYYRDSIAIGAFGAM 308
           ARIAAYKICW  GCFDSD+LAAMDQAIADGV++ISLSVG++G AP Y  DSIAIGAFGA 
Sbjct: 244 ARIAAYKICWSLGCFDSDLLAAMDQAIADGVNVISLSVGATGYAPQYDHDSIAIGAFGAA 303

Query: 309 QHGVVVSCSAGNSGPGSYTAVNIAPWILTVGASTIDREFLADVILGDGRVFSGVSLYSGE 368
           QHG+VVSCSAGNSGPG YTAVNIAPWILTVGASTIDREF AD ILGDGR+F GVSLYSG+
Sbjct: 304 QHGIVVSCSAGNSGPGPYTAVNIAPWILTVGASTIDREFPADAILGDGRIFGGVSLYSGD 363

Query: 369 PLGDSKLPLVYGGDCGSRYCYSGSLDSSKVAGKIVVCDRGGNARVAKGSAVKSAGGLGMV 428
           PL D KLPLVY GD G+RYCY GSL  SKV GKIV CDRGGNARV KG AVK AGGLGM+
Sbjct: 364 PLVDIKLPLVYAGDSGNRYCYMGSLSPSKVQGKIVFCDRGGNARVEKGFAVKLAGGLGMI 423

Query: 429 LANTEENGEELLADSHLIPGTMVGEIAGNKLRNYTHSDPNPTATIVFRGTVIGNSPSAPR 488
           LANT E+GEEL+AD+HLIP T VGE AGN++R Y      PTATIVF GTVIG SP AP+
Sbjct: 424 LANTAESGEELIADAHLIPATTVGEKAGNEIRQYIKISQFPTATIVFHGTVIGPSPPAPK 483

Query: 489 VAAFSSRGPNYRTAEILKPDVIAPGVNILAGWSGYSSPTSLSIDPRRVEFNIISGTSMSC 548
           VAAFSSRGPN+ T EILKPDVIAPGVNILAGW+G+  P+ L+ID RRV FNIISGTSMSC
Sbjct: 484 VAAFSSRGPNHLTPEILKPDVIAPGVNILAGWTGFIGPSQLNIDTRRVNFNIISGTSMSC 543

Query: 549 PHVSGVAALLRKAFPTWSPAAIKSALITTSYSLDSSGSPIKDLSTSKESNPFVHGAGHID 608
           PHVSG+AALL KA+P WSPAAIKSAL+TT+YSLD+SG+ IKDL+T  ES+PFV+GAGH+D
Sbjct: 544 PHVSGLAALLIKAYPNWSPAAIKSALMTTAYSLDNSGNTIKDLATGVESSPFVYGAGHVD 603

Query: 609 PNKALNPGLIYDLTPQDYVSFLCSIGYDSRQIAVFVKDSSYSQLCEHKLSNPGNLNYPSF 668
           PN AL PGL+YD+   DYV+FLCSIGYDS++IA+FV++ +   +CE KL+ PGNLNYPSF
Sbjct: 604 PNIALMPGLVYDIDDGDYVAFLCSIGYDSKRIAIFVREPTGPDVCEGKLATPGNLNYPSF 663

Query: 669 AVVFD-DEEVVKYTRTVTNVGDETDAVYEVKVESPQGVEISVVPNKLEFNEKKTTQSYEI 728
           +VVFD ++ VVKY RTV NVG   DAVYE KV +P GVEISV P+KLEF+ +  T SYEI
Sbjct: 664 SVVFDSNDHVVKYKRTVKNVGPSVDAVYEAKVNAPPGVEISVSPSKLEFSAENQTLSYEI 723

Query: 729 TFTK--ISGFK-ELASFGSIQWSDGSHNVRSPIAVSFNTG 764
           TF    ++ F   L +FGSI+WSDG H VRSPIAV +  G
Sbjct: 724 TFASDGLALFAVALEAFGSIEWSDGVHLVRSPIAVRWLQG 762