ClCG09G001040 (gene) Watermelon (Charleston Gray)

NameClCG09G001040
Typegene
OrganismCitrullus lanatus (Watermelon (Charleston Gray))
DescriptionSubtilisin-like serine protease
LocationCG_Chr09 : 946394 .. 948697 (-)
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: CDS
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGGCGAATTTCCATCGCTCTTCTTCACTTTTCTTCTTCTTCATCTTCTGTTTCGTCCCTGTAATTTTCTCTCGATCGCCGGATAATCAAGAGACATTCATCGTTCATGTCTCTAAATCGGAGAAGCCTTCTTTATTTTCCTCCCATCGTCACTGGCACTCTTCCATTCTCGAATCACTCTCTCCTTCTCCTCATCCGACGAAGCTTCTTTACAACTACGAACATGCCGTTAATGGATTCTCCGCACGCATCACGGCTGCTCAGGCGGCGGAGCTGCGTCATGTTCCCGGTGTTATTTCGGTGATTCCTGATCGGATCCGTCAGCTTCATACGACTCGGACGCCTCATTTCTTGGGACTGGCGGATAACCTAGGGCTCTGGCCTGACACGAATTATGCCGAAGATGTGATTATTGGCGTTCTTGATACCGGAATTTGGCCGGGGAGGCCGAGTTTCTCCGATGAAGGTTTGTCTCCGGTTCCGGCAAGCTGGAAAGGCACCTGCGAGGCTGGGGAGGGATTTTCGGCTTCGACCTGTAATCGGAAGATTATTGGTGCGAGAGCGTACTTTAACGGATACGAGTCTAATTTAGGAAGATCGCTTCGTTCTTCGGATTTCAAATCTGCTAGGGATTCGGATGGCCATGGAACTCACACGGCCTCTACGGCTGCAGGTTCGTTCGTTAAAAATGCTAGTTTTTTCCAATACGCTCGTGGAGAAGCCAGAGGAATGGCGAGTAGAGCTCGTATCGCTGCCTACAAGATCTGCTGGGAATTTGGTTGTTTCGATTCTGACATACTCGCCGCTATGGATCAGGCGATTGCCGATGGCGTTCACATCATCTCTCTCTCAGTCGGATCCTCGGGCCGTGCGCCCGCGTACTATCGAGATTCCATTGCAATTGGAGCGTTTGGGGCAATGCAGCACGGTGTCGTCGTCTCTTGCTCCGCCGGTAATTCCGGTCCCGGTCCTTACACGGCTGTGAACATCGCTCCTTGGATCCTCACCGTCGGTGCCTCCACAATCGACAGGGAGTTTCTTGCCGATGTCATTCTCGGAGACGGCAGGGTTTTCAGCGGCGTGTCGCTGTACTCCGGCGAACCCCTTGGCGATTCCAAGCTTCCGTTGGTTTACGGTGGAGATTGCGGTAGCAGGTATTGTTATTCTGGATCCCTCGATCCGGCGAAAGTCGCAGGAAAAATTGTCGTGTGCGACCGAGGAGGGAACGCCAGAGTCGCCAAAGGAGGCGCAGTGAAGTCCGCCGGTGGTCTTGGTATGGTACTCGCCAATACGGAGGAAAACGGCGAAGAGCTCTTAGCAGATTCTCATCTTATTCCAGGAACAATGGTCGGTGAAATCGCCGGCAACAAGCTTAGAAACTACATCCATTCGGATCCGAATCCAACTGCTACAATCGTGTTTCGCGGAACTGTCATTGGCAATTCGCCTTCAGCTCCGAGAGTCGCCGCCTTCTCCAGCCGTGGTCCGAACTACCGGACGGCGGAGATTCTAAAACCAGATGTTATAGCTCCGGGAGTTAACATTTTGGCCGGCTGGAGCGGCTACAGCAGTCCGACCAGTCTAAGCATCGATTCTCGAAGAGTCGAGTTCAATATCATCTCCGGAACTTCAATGTCTTGCCCTCACGTCAGTGGCGTCGCCGCCTTGCTCCGTAAGGCTTTTCCGACGTGGTCACCGGCTGCTATCAAATCTGCTCTGATAACCACTTCTTACTCTTTGGACAGTTCCGGCAGCCCAATCAAAGATCTCTCCACCAGCAAAGAATCAAATCCATTCGTCCATGGCGCCGGCCACATCGATCCCAACAAAGCTCTGAATCCGGGCCTGATTTATGATCTCACTCCTCAAGACTACGTTTCATTTCTCTGCTCAATCGGTTACGACTCTAGACAAATAGCTGTTTTCGTGAAGGATTCTTCATACTCCCAACTATGTGAGCACAAACTGAGCAATCCAGGCAACCTGAATTACCCATCATTCTCTGTCGTGTTCGACGGCGAGGAGGTGGTGAAATATACAAGAACAGTGACAAATGTAGGAGATGAAACTGATGTTTATGAAGTGAAAGTAGAATCCCCACAAGGAGTTGAAATCAGTGTTCTTCCCAACAAGCTGGAGTTCAATGCAAAGAAGACAACACAGTCTTATGAGATTACATTCACCAAAATCAGTGGATTCAAGGAATCAGCAAGCTTCGGATCAATTCAATGGAGTGATGGAAGCCACAATGTTAGGAGTCCAATTGCTGTCTCGTTCAACACTGGATCCATAGCTTCGATGTAA

mRNA sequence

ATGGCGAATTTCCATCGCTCTTCTTCACTTTTCTTCTTCTTCATCTTCTGTTTCGTCCCTGTAATTTTCTCTCGATCGCCGGATAATCAAGAGACATTCATCGTTCATGTCTCTAAATCGGAGAAGCCTTCTTTATTTTCCTCCCATCGTCACTGGCACTCTTCCATTCTCGAATCACTCTCTCCTTCTCCTCATCCGACGAAGCTTCTTTACAACTACGAACATGCCGTTAATGGATTCTCCGCACGCATCACGGCTGCTCAGGCGGCGGAGCTGCGTCATGTTCCCGGTGTTATTTCGGTGATTCCTGATCGGATCCGTCAGCTTCATACGACTCGGACGCCTCATTTCTTGGGACTGGCGGATAACCTAGGGCTCTGGCCTGACACGAATTATGCCGAAGATGTGATTATTGGCGTTCTTGATACCGGAATTTGGCCGGGGAGGCCGAGTTTCTCCGATGAAGGTTTGTCTCCGGTTCCGGCAAGCTGGAAAGGCACCTGCGAGGCTGGGGAGGGATTTTCGGCTTCGACCTGTAATCGGAAGATTATTGGTGCGAGAGCGTACTTTAACGGATACGAGTCTAATTTAGGAAGATCGCTTCGTTCTTCGGATTTCAAATCTGCTAGGGATTCGGATGGCCATGGAACTCACACGGCCTCTACGGCTGCAGGTTCGTTCGTTAAAAATGCTAGTTTTTTCCAATACGCTCGTGGAGAAGCCAGAGGAATGGCGAGTAGAGCTCGTATCGCTGCCTACAAGATCTGCTGGGAATTTGGTTGTTTCGATTCTGACATACTCGCCGCTATGGATCAGGCGATTGCCGATGGCGTTCACATCATCTCTCTCTCAGTCGGATCCTCGGGCCGTGCGCCCGCGTACTATCGAGATTCCATTGCAATTGGAGCGTTTGGGGCAATGCAGCACGGTGTCGTCGTCTCTTGCTCCGCCGGTAATTCCGGTCCCGGTCCTTACACGGCTGTGAACATCGCTCCTTGGATCCTCACCGTCGGTGCCTCCACAATCGACAGGGAGTTTCTTGCCGATGTCATTCTCGGAGACGGCAGGGTTTTCAGCGGCGTGTCGCTGTACTCCGGCGAACCCCTTGGCGATTCCAAGCTTCCGTTGGTTTACGGTGGAGATTGCGGTAGCAGGTATTGTTATTCTGGATCCCTCGATCCGGCGAAAGTCGCAGGAAAAATTGTCGTGTGCGACCGAGGAGGGAACGCCAGAGTCGCCAAAGGAGGCGCAGTGAAGTCCGCCGGTGGTCTTGGTATGGTACTCGCCAATACGGAGGAAAACGGCGAAGAGCTCTTAGCAGATTCTCATCTTATTCCAGGAACAATGGTCGGTGAAATCGCCGGCAACAAGCTTAGAAACTACATCCATTCGGATCCGAATCCAACTGCTACAATCGTGTTTCGCGGAACTGTCATTGGCAATTCGCCTTCAGCTCCGAGAGTCGCCGCCTTCTCCAGCCGTGGTCCGAACTACCGGACGGCGGAGATTCTAAAACCAGATGTTATAGCTCCGGGAGTTAACATTTTGGCCGGCTGGAGCGGCTACAGCAGTCCGACCAGTCTAAGCATCGATTCTCGAAGAGTCGAGTTCAATATCATCTCCGGAACTTCAATGTCTTGCCCTCACGTCAGTGGCGTCGCCGCCTTGCTCCGTAAGGCTTTTCCGACGTGGTCACCGGCTGCTATCAAATCTGCTCTGATAACCACTTCTTACTCTTTGGACAGTTCCGGCAGCCCAATCAAAGATCTCTCCACCAGCAAAGAATCAAATCCATTCGTCCATGGCGCCGGCCACATCGATCCCAACAAAGCTCTGAATCCGGGCCTGATTTATGATCTCACTCCTCAAGACTACGTTTCATTTCTCTGCTCAATCGGTTACGACTCTAGACAAATAGCTGTTTTCGTGAAGGATTCTTCATACTCCCAACTATGTGAGCACAAACTGAGCAATCCAGGCAACCTGAATTACCCATCATTCTCTGTCGTGTTCGACGGCGAGGAGGTGGTGAAATATACAAGAACAGTGACAAATGTAGGAGATGAAACTGATGTTTATGAAGTGAAAGTAGAATCCCCACAAGGAGTTGAAATCAGTGTTCTTCCCAACAAGCTGGAGTTCAATGCAAAGAAGACAACACAGTCTTATGAGATTACATTCACCAAAATCAGTGGATTCAAGGAATCAGCAAGCTTCGGATCAATTCAATGGAGTGATGGAAGCCACAATGTTAGGAGTCCAATTGCTGTCTCGTTCAACACTGGATCCATAGCTTCGATGTAA

Coding sequence (CDS)

ATGGCGAATTTCCATCGCTCTTCTTCACTTTTCTTCTTCTTCATCTTCTGTTTCGTCCCTGTAATTTTCTCTCGATCGCCGGATAATCAAGAGACATTCATCGTTCATGTCTCTAAATCGGAGAAGCCTTCTTTATTTTCCTCCCATCGTCACTGGCACTCTTCCATTCTCGAATCACTCTCTCCTTCTCCTCATCCGACGAAGCTTCTTTACAACTACGAACATGCCGTTAATGGATTCTCCGCACGCATCACGGCTGCTCAGGCGGCGGAGCTGCGTCATGTTCCCGGTGTTATTTCGGTGATTCCTGATCGGATCCGTCAGCTTCATACGACTCGGACGCCTCATTTCTTGGGACTGGCGGATAACCTAGGGCTCTGGCCTGACACGAATTATGCCGAAGATGTGATTATTGGCGTTCTTGATACCGGAATTTGGCCGGGGAGGCCGAGTTTCTCCGATGAAGGTTTGTCTCCGGTTCCGGCAAGCTGGAAAGGCACCTGCGAGGCTGGGGAGGGATTTTCGGCTTCGACCTGTAATCGGAAGATTATTGGTGCGAGAGCGTACTTTAACGGATACGAGTCTAATTTAGGAAGATCGCTTCGTTCTTCGGATTTCAAATCTGCTAGGGATTCGGATGGCCATGGAACTCACACGGCCTCTACGGCTGCAGGTTCGTTCGTTAAAAATGCTAGTTTTTTCCAATACGCTCGTGGAGAAGCCAGAGGAATGGCGAGTAGAGCTCGTATCGCTGCCTACAAGATCTGCTGGGAATTTGGTTGTTTCGATTCTGACATACTCGCCGCTATGGATCAGGCGATTGCCGATGGCGTTCACATCATCTCTCTCTCAGTCGGATCCTCGGGCCGTGCGCCCGCGTACTATCGAGATTCCATTGCAATTGGAGCGTTTGGGGCAATGCAGCACGGTGTCGTCGTCTCTTGCTCCGCCGGTAATTCCGGTCCCGGTCCTTACACGGCTGTGAACATCGCTCCTTGGATCCTCACCGTCGGTGCCTCCACAATCGACAGGGAGTTTCTTGCCGATGTCATTCTCGGAGACGGCAGGGTTTTCAGCGGCGTGTCGCTGTACTCCGGCGAACCCCTTGGCGATTCCAAGCTTCCGTTGGTTTACGGTGGAGATTGCGGTAGCAGGTATTGTTATTCTGGATCCCTCGATCCGGCGAAAGTCGCAGGAAAAATTGTCGTGTGCGACCGAGGAGGGAACGCCAGAGTCGCCAAAGGAGGCGCAGTGAAGTCCGCCGGTGGTCTTGGTATGGTACTCGCCAATACGGAGGAAAACGGCGAAGAGCTCTTAGCAGATTCTCATCTTATTCCAGGAACAATGGTCGGTGAAATCGCCGGCAACAAGCTTAGAAACTACATCCATTCGGATCCGAATCCAACTGCTACAATCGTGTTTCGCGGAACTGTCATTGGCAATTCGCCTTCAGCTCCGAGAGTCGCCGCCTTCTCCAGCCGTGGTCCGAACTACCGGACGGCGGAGATTCTAAAACCAGATGTTATAGCTCCGGGAGTTAACATTTTGGCCGGCTGGAGCGGCTACAGCAGTCCGACCAGTCTAAGCATCGATTCTCGAAGAGTCGAGTTCAATATCATCTCCGGAACTTCAATGTCTTGCCCTCACGTCAGTGGCGTCGCCGCCTTGCTCCGTAAGGCTTTTCCGACGTGGTCACCGGCTGCTATCAAATCTGCTCTGATAACCACTTCTTACTCTTTGGACAGTTCCGGCAGCCCAATCAAAGATCTCTCCACCAGCAAAGAATCAAATCCATTCGTCCATGGCGCCGGCCACATCGATCCCAACAAAGCTCTGAATCCGGGCCTGATTTATGATCTCACTCCTCAAGACTACGTTTCATTTCTCTGCTCAATCGGTTACGACTCTAGACAAATAGCTGTTTTCGTGAAGGATTCTTCATACTCCCAACTATGTGAGCACAAACTGAGCAATCCAGGCAACCTGAATTACCCATCATTCTCTGTCGTGTTCGACGGCGAGGAGGTGGTGAAATATACAAGAACAGTGACAAATGTAGGAGATGAAACTGATGTTTATGAAGTGAAAGTAGAATCCCCACAAGGAGTTGAAATCAGTGTTCTTCCCAACAAGCTGGAGTTCAATGCAAAGAAGACAACACAGTCTTATGAGATTACATTCACCAAAATCAGTGGATTCAAGGAATCAGCAAGCTTCGGATCAATTCAATGGAGTGATGGAAGCCACAATGTTAGGAGTCCAATTGCTGTCTCGTTCAACACTGGATCCATAGCTTCGATGTAA

Protein sequence

MANFHRSSSLFFFFIFCFVPVIFSRSPDNQETFIVHVSKSEKPSLFSSHRHWHSSILESLSPSPHPTKLLYNYEHAVNGFSARITAAQAAELRHVPGVISVIPDRIRQLHTTRTPHFLGLADNLGLWPDTNYAEDVIIGVLDTGIWPGRPSFSDEGLSPVPASWKGTCEAGEGFSASTCNRKIIGARAYFNGYESNLGRSLRSSDFKSARDSDGHGTHTASTAAGSFVKNASFFQYARGEARGMASRARIAAYKICWEFGCFDSDILAAMDQAIADGVHIISLSVGSSGRAPAYYRDSIAIGAFGAMQHGVVVSCSAGNSGPGPYTAVNIAPWILTVGASTIDREFLADVILGDGRVFSGVSLYSGEPLGDSKLPLVYGGDCGSRYCYSGSLDPAKVAGKIVVCDRGGNARVAKGGAVKSAGGLGMVLANTEENGEELLADSHLIPGTMVGEIAGNKLRNYIHSDPNPTATIVFRGTVIGNSPSAPRVAAFSSRGPNYRTAEILKPDVIAPGVNILAGWSGYSSPTSLSIDSRRVEFNIISGTSMSCPHVSGVAALLRKAFPTWSPAAIKSALITTSYSLDSSGSPIKDLSTSKESNPFVHGAGHIDPNKALNPGLIYDLTPQDYVSFLCSIGYDSRQIAVFVKDSSYSQLCEHKLSNPGNLNYPSFSVVFDGEEVVKYTRTVTNVGDETDVYEVKVESPQGVEISVLPNKLEFNAKKTTQSYEITFTKISGFKESASFGSIQWSDGSHNVRSPIAVSFNTGSIASM
BLAST of ClCG09G001040 vs. Swiss-Prot
Match: SBT14_ARATH (Subtilisin-like protease SBT1.4 OS=Arabidopsis thaliana GN=SBT1.4 PE=2 SV=1)

HSP 1 Score: 988.0 bits (2553), Expect = 5.7e-287
Identity = 493/771 (63.94%), Postives = 590/771 (76.52%), Query Frame = 1

Query: 8   SSLFFFF--IFCFVPVIFSRSPDNQETFIVHVSKSEKPSLFSSHRHWHSSILESLSPSPH 67
           SS+FF F  + CF     S S D  E++IVHV +S KPSLFSSH +WH S+L SL  SP 
Sbjct: 7   SSIFFVFPLLLCFFSPS-SSSSDGLESYIVHVQRSHKPSLFSSHNNWHVSLLRSLPSSPQ 66

Query: 68  PTKLLYNYEHAVNGFSARITAAQAAELRHVPGVISVIPDRIRQLHTTRTPHFLGLADNLG 127
           P  LLY+Y  AV+GFSAR++  Q A LR  P VISVIPD+ R++HTT TP FLG + N G
Sbjct: 67  PATLLYSYSRAVHGFSARLSPIQTAALRRHPSVISVIPDQAREIHTTHTPAFLGFSQNSG 126

Query: 128 LWPDTNYAEDVIIGVLDTGIWPGRPSFSDEGLSPVPASWKGTCEAGEGFSASTCNRKIIG 187
           LW ++NY EDVI+GVLDTGIWP  PSFSD GL P+P++WKG CE G  F AS+CNRK+IG
Sbjct: 127 LWSNSNYGEDVIVGVLDTGIWPEHPSFSDSGLGPIPSTWKGECEIGPDFPASSCNRKLIG 186

Query: 188 ARAYFNGY--ESNLGRSLRSSDFKSARDSDGHGTHTASTAAGSFVKNASFFQYARGEARG 247
           ARA++ GY  + N  +   + + +S RD++GHGTHTASTAAGS V NAS +QYARG A G
Sbjct: 187 ARAFYRGYLTQRNGTKKHAAKESRSPRDTEGHGTHTASTAAGSVVANASLYQYARGTATG 246

Query: 248 MASRARIAAYKICWEFGCFDSDILAAMDQAIADGVHIISLSVGSSGRAPAYYRDSIAIGA 307
           MAS+ARIAAYKICW  GC+DSDILAAMDQA+ADGVH+ISLSVG+SG AP Y+ DSIAIGA
Sbjct: 247 MASKARIAAYKICWTGGCYDSDILAAMDQAVADGVHVISLSVGASGSAPEYHTDSIAIGA 306

Query: 308 FGAMQHGVVVSCSAGNSGPGPYTAVNIAPWILTVGASTIDREFLADVILGDGRVFSGVSL 367
           FGA +HG+VVSCSAGNSGP P TA NIAPWILTVGAST+DREF A+ I GDG+VF+G SL
Sbjct: 307 FGATRHGIVVSCSAGNSGPNPETATNIAPWILTVGASTVDREFAANAITGDGKVFTGTSL 366

Query: 368 YSGEPLGDSKLPLVYGGDCGSRYCYSGSLDPAKVAGKIVVCDRGGNARVAKGGAVKSAGG 427
           Y+GE L DS+L LVY GDCGSR CY G L+ + V GKIV+CDRGGNARV KG AVK AGG
Sbjct: 367 YAGESLPDSQLSLVYSGDCGSRLCYPGKLNSSLVEGKIVLCDRGGNARVEKGSAVKLAGG 426

Query: 428 LGMVLANTEENGEELLADSHLIPGTMVGEIAGNKLRNYIHSDPNPTATIVFRGTVIGNSP 487
            GM+LANT E+GEEL ADSHL+P TMVG  AG+++R+YI +  +PTA I F GT+IG SP
Sbjct: 427 AGMILANTAESGEELTADSHLVPATMVGAKAGDQIRDYIKTSDSPTAKISFLGTLIGPSP 486

Query: 488 SAPRVAAFSSRGPNYRTAEILKPDVIAPGVNILAGWSGYSSPTSLSIDSRRVEFNIISGT 547
            +PRVAAFSSRGPN+ T  ILKPDVIAPGVNILAGW+G   PT L ID RRV+FNIISGT
Sbjct: 487 PSPRVAAFSSRGPNHLTPVILKPDVIAPGVNILAGWTGMVGPTDLDIDPRRVQFNIISGT 546

Query: 548 SMSCPHVSGVAALLRKAFPTWSPAAIKSALITTSYSLDSSGSPIKDLSTSKESNPFVHGA 607
           SMSCPHVSG+AALLRKA P WSPAAIKSAL+TT+Y +++SG PI+DL+T K SN F+HGA
Sbjct: 547 SMSCPHVSGLAALLRKAHPDWSPAAIKSALVTTAYDVENSGEPIEDLATGKSSNSFIHGA 606

Query: 608 GHIDPNKALNPGLIYDLTPQDYVSFLCSIGYDSRQIAVFVKDSSYSQLCE-HKLSNPGNL 667
           GH+DPNKALNPGL+YD+  ++YV+FLC++GY+   I VF++D +    CE  KL   G+L
Sbjct: 607 GHVDPNKALNPGLVYDIEVKEYVAFLCAVGYEFPGILVFLQDPTLYDACETSKLRTAGDL 666

Query: 668 NYPSFSVVFDGE-EVVKYTRTVTNVGDETD-VYEVKVESPQGVEISVLPNKLEFNAKKTT 727
           NYPSFSVVF    EVVKY R V NVG   D VYEV V+SP  VEI V P+KL F+ +K+ 
Sbjct: 667 NYPSFSVVFASTGEVVKYKRVVKNVGSNVDAVYEVGVKSPANVEIDVSPSKLAFSKEKSV 726

Query: 728 QSYEITFTKI-----SGFKESASFGSIQWSDGSHNVRSPIAVSFNTGSIAS 767
             YE+TF  +      G      FGSI+W+DG H V+SP+AV +  GS+ S
Sbjct: 727 LEYEVTFKSVVLGGGVGSVPGHEFGSIEWTDGEHVVKSPVAVQWGQGSVQS 776

BLAST of ClCG09G001040 vs. Swiss-Prot
Match: SBT17_ARATH (Subtilisin-like protease SBT1.7 OS=Arabidopsis thaliana GN=SBT1.7 PE=1 SV=1)

HSP 1 Score: 815.1 bits (2104), Expect = 6.6e-235
Identity = 426/760 (56.05%), Postives = 531/760 (69.87%), Query Frame = 1

Query: 8   SSLFFFFIFCFVPVIFSRSPDNQETFIVHVSKSEKPSLFSSHRHWHSSILESLSPSPHPT 67
           SS  FF + C      S S  +Q T+IVH++KS+ PS F  H +W+ S L S+S S    
Sbjct: 7   SSTAFFLLLCLGFCHVSSSSSDQGTYIVHMAKSQMPSSFDLHSNWYDSSLRSISDS---A 66

Query: 68  KLLYNYEHAVNGFSARITAAQAAELRHVPGVISVIPDRIRQLHTTRTPHFLGLADNLG-L 127
           +LLY YE+A++GFS R+T  +A  L   PGVISV+P+   +LHTTRTP FLGL ++   L
Sbjct: 67  ELLYTYENAIHGFSTRLTQEEADSLMTQPGVISVLPEHRYELHTTRTPLFLGLDEHTADL 126

Query: 128 WPDTNYAEDVIIGVLDTGIWPGRPSFSDEGLSPVPASWKGTCEAGEGFSASTCNRKIIGA 187
           +P+     DV++GVLDTG+WP   S+SDEG  P+P+SWKG CEAG  F+AS CNRK+IGA
Sbjct: 127 FPEAGSYSDVVVGVLDTGVWPESKSYSDEGFGPIPSSWKGGCEAGTNFTASLCNRKLIGA 186

Query: 188 RAYFNGYESNLGRSLRSSDFKSARDSDGHGTHTASTAAGSFVKNASFFQYARGEARGMAS 247
           R +  GYES +G    S + +S RD DGHGTHT+STAAGS V+ AS   YA G ARGMA 
Sbjct: 187 RFFARGYESTMGPIDESKESRSPRDDDGHGTHTSSTAAGSVVEGASLLGYASGTARGMAP 246

Query: 248 RARIAAYKICWEFGCFDSDILAAMDQAIADGVHIISLSVGSSGRAPAYYRDSIAIGAFGA 307
           RAR+A YK+CW  GCF SDILAA+D+AIAD V+++S+S+G  G    YYRD +AIGAF A
Sbjct: 247 RARVAVYKVCWLGGCFSSDILAAIDKAIADNVNVLSMSLG--GGMSDYYRDGVAIGAFAA 306

Query: 308 MQHGVVVSCSAGNSGPGPYTAVNIAPWILTVGASTIDREFLADVILGDGRVFSGVSLYSG 367
           M+ G++VSCSAGN+GP   +  N+APWI TVGA T+DR+F A  ILG+G+ F+GVSL+ G
Sbjct: 307 MERGILVSCSAGNAGPSSSSLSNVAPWITTVGAGTLDRDFPALAILGNGKNFTGVSLFKG 366

Query: 368 EPLGDSKLPLVYGGDCGS----RYCYSGSLDPAKVAGKIVVCDRGGNARVAKGGAVKSAG 427
           E L D  LP +Y G+  +      C +G+L P KV GKIV+CDRG NARV KG  VK+AG
Sbjct: 367 EALPDKLLPFIYAGNASNATNGNLCMTGTLIPEKVKGKIVMCDRGINARVQKGDVVKAAG 426

Query: 428 GLGMVLANTEENGEELLADSHLIPGTMVGEIAGNKLRNYIHSDPNPTATIVFRGTVIGNS 487
           G+GM+LANT  NGEEL+AD+HL+P T VGE AG+ +R+Y+ +DPNPTA+I   GTV+G  
Sbjct: 427 GVGMILANTAANGEELVADAHLLPATTVGEKAGDIIRHYVTTDPNPTASISILGTVVGVK 486

Query: 488 PSAPRVAAFSSRGPNYRTAEILKPDVIAPGVNILAGWSGYSSPTSLSIDSRRVEFNIISG 547
           PS P VAAFSSRGPN  T  ILKPD+IAPGVNILA W+G + PT L+ DSRRVEFNIISG
Sbjct: 487 PS-PVVAAFSSRGPNSITPNILKPDLIAPGVNILAAWTGAAGPTGLASDSRRVEFNIISG 546

Query: 548 TSMSCPHVSGVAALLRKAFPTWSPAAIKSALITTSYSLDSSGSPIKDLSTSKESNPFVHG 607
           TSMSCPHVSG+AALL+   P WSPAAI+SAL+TT+Y     G P+ D++T K S PF HG
Sbjct: 547 TSMSCPHVSGLAALLKSVHPEWSPAAIRSALMTTAYKTYKDGKPLLDIATGKPSTPFDHG 606

Query: 608 AGHIDPNKALNPGLIYDLTPQDYVSFLCSIGYDSRQIAVFVKDSSYSQLCE-HKLSNPGN 667
           AGH+ P  A NPGLIYDLT +DY+ FLC++ Y S QI      S  +  C+  K  +  +
Sbjct: 607 AGHVSPTTATNPGLIYDLTTEDYLGFLCALNYTSPQIR---SVSRRNYTCDPSKSYSVAD 666

Query: 668 LNYPSFSVVFDGEEVVKYTRTVTNVGDETDVYEVKVES-PQGVEISVLPNKLEFNAKKTT 727
           LNYPSF+V  DG    KYTRTVT+VG     Y VKV S   GV+ISV P  L F      
Sbjct: 667 LNYPSFAVNVDGVGAYKYTRTVTSVGG-AGTYSVKVTSETTGVKISVEPAVLNFKEANEK 726

Query: 728 QSYEITFTKISGFKE-SASFGSIQWSDGSHNVRSPIAVSF 760
           +SY +TFT  S     S SFGSI+WSDG H V SP+A+S+
Sbjct: 727 KSYTVTFTVDSSKPSGSNSFGSIEWSDGKHVVGSPVAISW 756

BLAST of ClCG09G001040 vs. Swiss-Prot
Match: SBT18_ARATH (Subtilisin-like protease SBT1.8 OS=Arabidopsis thaliana GN=SBT1.8 PE=2 SV=1)

HSP 1 Score: 765.8 bits (1976), Expect = 4.6e-220
Identity = 395/737 (53.60%), Postives = 501/737 (67.98%), Query Frame = 1

Query: 30  QETFIVHVSKSEKPSLFSSHRHWHSSILESLSPSPHPTKLLYNYEHAVNGFSARITAAQA 89
           ++T+I+ V+ S+KP  F +H  W++S L S S       LLY Y  + +GFSA + + +A
Sbjct: 27  KKTYIIRVNHSDKPESFLTHHDWYTSQLNSES------SLLYTYTTSFHGFSAYLDSTEA 86

Query: 90  AELRHVPG-VISVIPDRIRQLHTTRTPHFLGLADNLGLWPDTNYAEDVIIGVLDTGIWPG 149
             L      ++ +  D +  LHTTRTP FLGL    G+    + +  VIIGVLDTG+WP 
Sbjct: 87  DSLLSSSNSILDIFEDPLYTLHTTRTPEFLGLNSEFGVHDLGSSSNGVIIGVLDTGVWPE 146

Query: 150 RPSFSDEGLSPVPASWKGTCEAGEGFSASTCNRKIIGARAYFNGYESNLGRSLRSS-DFK 209
             SF D  +  +P+ WKG CE+G  F +  CN+K+IGAR++  G++   G    S  +  
Sbjct: 147 SRSFDDTDMPEIPSKWKGECESGSDFDSKLCNKKLIGARSFSKGFQMASGGGFSSKRESV 206

Query: 210 SARDSDGHGTHTASTAAGSFVKNASFFQYARGEARGMASRARIAAYKICWEFGCFDSDIL 269
           S RD DGHGTHT++TAAGS V+NASF  YA G ARGMA+RAR+A YK+CW  GCF SDIL
Sbjct: 207 SPRDVDGHGTHTSTTAAGSAVRNASFLGYAAGTARGMATRARVATYKVCWSTGCFGSDIL 266

Query: 270 AAMDQAIADGVHIISLSVGSSGRAPAYYRDSIAIGAFGAMQHGVVVSCSAGNSGPGPYTA 329
           AAMD+AI DGV ++SLS+G  G AP YYRD+IAIGAF AM+ GV VSCSAGNSGP   + 
Sbjct: 267 AAMDRAILDGVDVLSLSLGG-GSAP-YYRDTIAIGAFSAMERGVFVSCSAGNSGPTRASV 326

Query: 330 VNIAPWILTVGASTIDREFLADVILGDGRVFSGVSLYSGEPLGDSKLPLVY--GGDCGSR 389
            N+APW++TVGA T+DR+F A   LG+G+  +GVSLYSG  +G   L LVY  G    S 
Sbjct: 327 ANVAPWVMTVGAGTLDRDFPAFANLGNGKRLTGVSLYSGVGMGTKPLELVYNKGNSSSSN 386

Query: 390 YCYSGSLDPAKVAGKIVVCDRGGNARVAKGGAVKSAGGLGMVLANTEENGEELLADSHLI 449
            C  GSLD + V GKIVVCDRG NARV KG  V+ AGGLGM++ANT  +GEEL+ADSHL+
Sbjct: 387 LCLPGSLDSSIVRGKIVVCDRGVNARVEKGAVVRDAGGLGMIMANTAASGEELVADSHLL 446

Query: 450 PGTMVGEIAGNKLRNYIHSDPNPTATIVFRGTVIGNSPSAPRVAAFSSRGPNYRTAEILK 509
           P   VG+  G+ LR Y+ SD  PTA +VF+GTV+   PS P VAAFSSRGPN  T EILK
Sbjct: 447 PAIAVGKKTGDLLREYVKSDSKPTALLVFKGTVLDVKPS-PVVAAFSSRGPNTVTPEILK 506

Query: 510 PDVIAPGVNILAGWSGYSSPTSLSIDSRRVEFNIISGTSMSCPHVSGVAALLRKAFPTWS 569
           PDVI PGVNILAGWS    PT L  DSRR +FNI+SGTSMSCPH+SG+A LL+ A P WS
Sbjct: 507 PDVIGPGVNILAGWSDAIGPTGLDKDSRRTQFNIMSGTSMSCPHISGLAGLLKAAHPEWS 566

Query: 570 PAAIKSALITTSYSLDSSGSPIKDLSTSKESNPFVHGAGHIDPNKALNPGLIYDLTPQDY 629
           P+AIKSAL+TT+Y LD++ +P+ D + +  SNP+ HG+GH+DP KAL+PGL+YD++ ++Y
Sbjct: 567 PSAIKSALMTTAYVLDNTNAPLHDAADNSLSNPYAHGSGHVDPQKALSPGLVYDISTEEY 626

Query: 630 VSFLCSIGYDSRQIAVFVKDSSYSQLCEHKLSNPGNLNYPSFSVVFDGEEVVKYTRTVTN 689
           + FLCS+ Y    I   VK  S +  C  K S+PG LNYPSFSV+F G+ VV+YTR VTN
Sbjct: 627 IRFLCSLDYTVDHIVAIVKRPSVN--CSKKFSDPGQLNYPSFSVLFGGKRVVRYTREVTN 686

Query: 690 VGDETDVYEVKVESPQGVEISVLPNKLEFNAKKTTQSYEITFTKISG--FKESASFGSIQ 749
           VG  + VY+V V     V ISV P+KL F +    + Y +TF    G      A FGSI 
Sbjct: 687 VGAASSVYKVTVNGAPSVGISVKPSKLSFKSVGEKKRYTVTFVSKKGVSMTNKAEFGSIT 746

Query: 750 WSDGSHNVRSPIAVSFN 761
           WS+  H VRSP+A S+N
Sbjct: 747 WSNPQHEVRSPVAFSWN 752

BLAST of ClCG09G001040 vs. Swiss-Prot
Match: SBT15_ARATH (Subtilisin-like protease SBT1.5 OS=Arabidopsis thaliana GN=SBT1.5 PE=2 SV=1)

HSP 1 Score: 758.8 bits (1958), Expect = 5.6e-218
Identity = 395/773 (51.10%), Postives = 514/773 (66.49%), Query Frame = 1

Query: 11  FFFFIFCFVPVIFSRSPDNQETFIVHVSKSEKPSLFSSHRHWHSSILESLSPSPHPTKLL 70
           +FFF+        S S  N  T+IVHV    KPS+F +H HW++S L SL+ SP    ++
Sbjct: 6   YFFFLLTLSSPSSSASSSNSLTYIVHVDHEAKPSIFPTHFHWYTSSLASLTSSP--PSII 65

Query: 71  YNYEHAVNGFSARITAAQAAELRHVPGVISVIPDRIRQLHTTRTPHFLGL--ADNLGLWP 130
           + Y+   +GFSAR+T+  A++L   P VISVIP+++R LHTTR+P FLGL   D  GL  
Sbjct: 66  HTYDTVFHGFSARLTSQDASQLLDHPHVISVIPEQVRHLHTTRSPEFLGLRSTDKAGLLE 125

Query: 131 DTNYAEDVIIGVLDTGIWPGRPSFSDEGLSPVPASWKGTCEAGEGFSASTCNRKIIGARA 190
           ++++  D++IGV+DTG+WP RPSF D GL PVP  WKG C A + F  S CNRK++GAR 
Sbjct: 126 ESDFGSDLVIGVIDTGVWPERPSFDDRGLGPVPIKWKGQCIASQDFPESACNRKLVGARF 185

Query: 191 YFNGYESNLGRSLRSSDFKSARDSDGHGTHTASTAAGSFVKNASFFQYARGEARGMASRA 250
           +  GYE+  G+   +++F+S RDSDGHGTHTAS +AG +V  AS   YA G A GMA +A
Sbjct: 186 FCGGYEATNGKMNETTEFRSPRDSDGHGTHTASISAGRYVFPASTLGYAHGVAAGMAPKA 245

Query: 251 RIAAYKICWEFGCFDSDILAAMDQAIADGVHIISLSVGSSGRAPAYYRDSIAIGAFGAMQ 310
           R+AAYK+CW  GC+DSDILAA D A+ADGV +ISLSVG  G    YY D+IAIGAFGA+ 
Sbjct: 246 RLAAYKVCWNSGCYDSDILAAFDTAVADGVDVISLSVG--GVVVPYYLDAIAIGAFGAID 305

Query: 311 HGVVVSCSAGNSGPGPYTAVNIAPWILTVGASTIDREFLADVILGDGRVFSGVSLYSGEP 370
            G+ VS SAGN GPG  T  N+APW+ TVGA TIDR+F A+V LG+G++ SGVS+Y G  
Sbjct: 306 RGIFVSASAGNGGPGALTVTNVAPWMTTVGAGTIDRDFPANVKLGNGKMISGVSVYGGPG 365

Query: 371 LGDSKL-PLVYGGDC------GSRYCYSGSLDPAKVAGKIVVCDRGGNARVAKGGAVKSA 430
           L   ++ PLVYGG         S  C  GSLDP  V GKIV+CDRG N+R  KG  V+  
Sbjct: 366 LDPGRMYPLVYGGSLLGGDGYSSSLCLEGSLDPNLVKGKIVLCDRGINSRATKGEIVRKN 425

Query: 431 GGLGMVLANTEENGEELLADSHLIPGTMVGEIAGNKLRNYI------HSDPNPTATIVFR 490
           GGLGM++AN   +GE L+AD H++P T VG   G+++R YI       S  +PTATIVF+
Sbjct: 426 GGLGMIIANGVFDGEGLVADCHVLPATSVGASGGDEIRRYISESSKSRSSKHPTATIVFK 485

Query: 491 GTVIGNSPSAPRVAAFSSRGPNYRTAEILKPDVIAPGVNILAGWSGYSSPTSLSIDSRRV 550
           GT +G  P AP VA+FS+RGPN  T EILKPDVIAPG+NILA W     P+ ++ D+RR 
Sbjct: 486 GTRLGIRP-APVVASFSARGPNPETPEILKPDVIAPGLNILAAWPDRIGPSGVTSDNRRT 545

Query: 551 EFNIISGTSMSCPHVSGVAALLRKAFPTWSPAAIKSALITTSYSLDSSGSPIKDLSTSKE 610
           EFNI+SGTSM+CPHVSG+AALL+ A P WSPAAI+SALITT+Y++D+SG P+ D ST   
Sbjct: 546 EFNILSGTSMACPHVSGLAALLKAAHPDWSPAAIRSALITTAYTVDNSGEPMMDESTGNT 605

Query: 611 SNPFVHGAGHIDPNKALNPGLIYDLTPQDYVSFLCSIGYDSRQIAVFVKDSSYSQLCE-- 670
           S+   +G+GH+ P KA++PGL+YD+T  DY++FLC+  Y    I    +  +    C+  
Sbjct: 606 SSVMDYGSGHVHPTKAMDPGLVYDITSYDYINFLCNSNYTRTNIVTITRRQAD---CDGA 665

Query: 671 HKLSNPGNLNYPSFSVVFD--GEEVVK--YTRTVTNVGDETDVYEVKVESPQGVEISVLP 730
            +  + GNLNYPSFSVVF   GE  +   + RTVTNVGD   VYE+K+  P+G  ++V P
Sbjct: 666 RRAGHVGNLNYPSFSVVFQQYGESKMSTHFIRTVTNVGDSDSVYEIKIRPPRGTTVTVEP 725

Query: 731 NKLEFNAKKTTQSYEI----TFTKISGFKESASFGSIQWSDGSHNVRSPIAVS 759
            KL F       S+ +    T  K+S    +   G I WSDG  NV SP+ V+
Sbjct: 726 EKLSFRRVGQKLSFVVRVKTTEVKLSPGATNVETGHIVWSDGKRNVTSPLVVT 770

BLAST of ClCG09G001040 vs. Swiss-Prot
Match: SBT13_ARATH (Subtilisin-like protease SBT1.3 OS=Arabidopsis thaliana GN=SBT1.3 PE=2 SV=1)

HSP 1 Score: 726.1 bits (1873), Expect = 4.0e-208
Identity = 378/772 (48.96%), Postives = 505/772 (65.41%), Query Frame = 1

Query: 12  FFFIFCFVPVIFSRSP-----DNQETFIVHVSKSEKPSLFSSHRHWHSSILESLSPSP-- 71
           F FI   + +IF ++        ++T+++H+ KS  P  +++H  W+SS + S++     
Sbjct: 11  FLFIILSINLIFLQAETTTQISTKKTYVIHMDKSAMPLPYTNHLQWYSSKINSVTQHKSQ 70

Query: 72  ----HPTKLLYNYEHAVNGFSARITAAQAAELRHVPGVISVIPDRIRQLHTTRTPHFLGL 131
               +  ++LY Y+ A +G +A++T  +A  L    GV++VIP+   +LHTTR+P FLGL
Sbjct: 71  EEEGNNNRILYTYQTAFHGLAAQLTQEEAERLEEEDGVVAVIPETRYELHTTRSPTFLGL 130

Query: 132 A--DNLGLWPDTNYAEDVIIGVLDTGIWPGRPSFSDEGLSPVPASWKGTCEAGEGFSAST 191
              ++  +W +     DV++GVLDTGIWP   SF+D G+SPVPA+W+G CE G+ F    
Sbjct: 131 ERQESERVWAERVTDHDVVVGVLDTGIWPESESFNDTGMSPVPATWRGACETGKRFLKRN 190

Query: 192 CNRKIIGARAYFNGYESNLGRSLRSSDFKSARDSDGHGTHTASTAAGSFVKNASFFQYAR 251
           CNRKI+GAR ++ GYE+  G+     ++KS RD DGHGTHTA+T AGS VK A+ F +A 
Sbjct: 191 CNRKIVGARVFYRGYEAATGKIDEELEYKSPRDRDGHGTHTAATVAGSPVKGANLFGFAY 250

Query: 252 GEARGMASRARIAAYKICWEFGCFDSDILAAMDQAIADGVHIISLSVGSSGRAPAYYRDS 311
           G ARGMA +AR+AAYK+CW  GCF SDIL+A+DQA+ADGV ++S+S+G  G    Y RDS
Sbjct: 251 GTARGMAQKARVAAYKVCWVGGCFSSDILSAVDQAVADGVQVLSISLG--GGVSTYSRDS 310

Query: 312 IAIGAFGAMQHGVVVSCSAGNSGPGPYTAVNIAPWILTVGASTIDREFLADVILGDGRVF 371
           ++I  FGAM+ GV VSCSAGN GP P +  N++PWI TVGAST+DR+F A V +G  R F
Sbjct: 311 LSIATFGAMEMGVFVSCSAGNGGPDPISLTNVSPWITTVGASTMDRDFPATVKIGTMRTF 370

Query: 372 SGVSLYSGEPL--GDSKLPLVYGGDCGSR-----YCYSGSLDPAKVAGKIVVCDRGGNAR 431
            GVSLY G  +   + + PLVY G   S      +C  G+LD   VAGKIV+CDRG   R
Sbjct: 371 KGVSLYKGRTVLPKNKQYPLVYLGRNASSPDPTSFCLDGALDRRHVAGKIVICDRGVTPR 430

Query: 432 VAKGGAVKSAGGLGMVLANTEENGEELLADSHLIPGTMVGEIAGNKLRNYIHSDPNPTAT 491
           V KG  VK AGG+GMVL NT  NGEEL+ADSH++P   VGE  G  ++ Y  +    TA+
Sbjct: 431 VQKGQVVKRAGGIGMVLTNTATNGEELVADSHMLPAVAVGEKEGKLIKQYAMTSKKATAS 490

Query: 492 IVFRGTVIGNSPSAPRVAAFSSRGPNYRTAEILKPDVIAPGVNILAGWSGYSSPTSLSID 551
           +   GT IG  PS P VAAFSSRGPN+ + EILKPD++APGVNILA W+G  +P+SLS D
Sbjct: 491 LEILGTRIGIKPS-PVVAAFSSRGPNFLSLEILKPDLLAPGVNILAAWTGDMAPSSLSSD 550

Query: 552 SRRVEFNIISGTSMSCPHVSGVAALLRKAFPTWSPAAIKSALITTSYSLDSSGSPIKDLS 611
            RRV+FNI+SGTSMSCPHVSGVAAL++   P WSPAAIKSAL+TT+Y  D+   P+ D S
Sbjct: 551 PRRVKFNILSGTSMSCPHVSGVAALIKSRHPDWSPAAIKSALMTTAYVHDNMFKPLTDAS 610

Query: 612 TSKESNPFVHGAGHIDPNKALNPGLIYDLTPQDYVSFLCSIGYDSRQIAVFVKDSSYSQL 671
            +  S+P+ HGAGHIDP +A +PGL+YD+ PQ+Y  FLC+      Q+ VF K S  ++ 
Sbjct: 611 GAAPSSPYDHGAGHIDPLRATDPGLVYDIGPQEYFEFLCTQDLSPSQLKVFTKHS--NRT 670

Query: 672 CEHKLS-NPGNLNYPSFSVVFDGEEVVK---YTRTVTNVGDETDVYEVKVESPQGVEISV 731
           C+H L+ NPGNLNYP+ S +F     VK     RTVTNVG     Y+V V   +G  ++V
Sbjct: 671 CKHTLAKNPGNLNYPAISALFPENTHVKAMTLRRTVTNVGPHISSYKVSVSPFKGASVTV 730

Query: 732 LPNKLEFNAKKTTQSYEITFTKISGFKESASFGSIQWSDGSHNVRSPIAVSF 760
            P  L F +K    SY +TF      K    FG + W   +H VRSP+ +++
Sbjct: 731 QPKTLNFTSKHQKLSYTVTFRTRFRMKR-PEFGGLVWKSTTHKVRSPVIITW 776

BLAST of ClCG09G001040 vs. TrEMBL
Match: A0A0A0K6Z1_CUCSA (Subtilase family protein OS=Cucumis sativus GN=Csa_7G448910 PE=4 SV=1)

HSP 1 Score: 1393.3 bits (3605), Expect = 0.0e+00
Identity = 697/771 (90.40%), Postives = 728/771 (94.42%), Query Frame = 1

Query: 1   MANFHR-SSSLFFFFIFCFVPVIFSRSPDNQETFIVHVSKSEKPSLFSSHRHWHSSILES 60
           MANFH  SSS FFFF+FCF P+IFSRS +NQET+IVHVSKSEKPSLFSSH HWHSSILES
Sbjct: 1   MANFHLPSSSFFFFFLFCFFPLIFSRSLENQETYIVHVSKSEKPSLFSSHHHWHSSILES 60

Query: 61  LSPSPHPTKLLYNYEHAVNGFSARITAAQAAELRHVPGVISVIPDRIRQLHTTRTPHFLG 120
           LS SPHPTKLLYNYE A NGFSARIT  QA ELR VPG+ISVIPD+IRQLHTTRTPHFLG
Sbjct: 61  LSSSPHPTKLLYNYERAANGFSARITTVQAEELRRVPGIISVIPDQIRQLHTTRTPHFLG 120

Query: 121 LADNLGLWPDTNYAEDVIIGVLDTGIWPGRPSFSDEGLSPVPASWKGTCEAGEGFSASTC 180
           LADNLGLW DTNYA+DVIIGVLDTGIWP RPSFSDEGLSPVPA WKGTC+ GEG SA  C
Sbjct: 121 LADNLGLWADTNYADDVIIGVLDTGIWPERPSFSDEGLSPVPARWKGTCDTGEGVSAFAC 180

Query: 181 NRKIIGARAYFNGYESNLGRSLR-SSDFKSARDSDGHGTHTASTAAGSFVKNASFFQYAR 240
           NRKIIGARAYF GYESNL  SL+ SSDFKSARD++GHGTHTASTAAGSFV NASFFQYAR
Sbjct: 181 NRKIIGARAYFYGYESNLRGSLKVSSDFKSARDTEGHGTHTASTAAGSFVNNASFFQYAR 240

Query: 241 GEARGMASRARIAAYKICWEFGCFDSDILAAMDQAIADGVHIISLSVGSSGRAPAYYRDS 300
           GEARGMASRARIAAYKICWEFGC+DSDILAAMDQAI+DGV +ISLSVGSSGRAPAYYRDS
Sbjct: 241 GEARGMASRARIAAYKICWEFGCYDSDILAAMDQAISDGVDVISLSVGSSGRAPAYYRDS 300

Query: 301 IAIGAFGAMQHGVVVSCSAGNSGPGPYTAVNIAPWILTVGASTIDREFLADVILGDGRVF 360
           IAIGAFGAMQHGVVVSCSAGNSGPGPYTAVNIAPWILTVGASTIDREFLADVILGDGRVF
Sbjct: 301 IAIGAFGAMQHGVVVSCSAGNSGPGPYTAVNIAPWILTVGASTIDREFLADVILGDGRVF 360

Query: 361 SGVSLYSGEPLGDSKLPLVYGGDCGSRYCYSGSLDPAKVAGKIVVCDRGGNARVAKGGAV 420
           SGVSLYSG+PLGDSKL LVYGGDCGSRYCYSGSLD +KVAGKIVVCDRGGNARVAKGGAV
Sbjct: 361 SGVSLYSGDPLGDSKLQLVYGGDCGSRYCYSGSLDSSKVAGKIVVCDRGGNARVAKGGAV 420

Query: 421 KSAGGLGMVLANTEENGEELLADSHLIPGTMVGEIAGNKLRNYIHSDPNPTATIVFRGTV 480
           KSAGGLGMVLANTEENGEELLADSHLIPGTMVG IAGNKLR+YIH+DPNPTATIVFRGTV
Sbjct: 421 KSAGGLGMVLANTEENGEELLADSHLIPGTMVGAIAGNKLRDYIHTDPNPTATIVFRGTV 480

Query: 481 IGNSPSAPRVAAFSSRGPNYRTAEILKPDVIAPGVNILAGWSGYSSPTSLSIDSRRVEFN 540
           IG+SP APRVAAFSSRGPNYRTAEILKPDVIAPGVNILAGWSGYSSPT L+ID RRVEFN
Sbjct: 481 IGDSPPAPRVAAFSSRGPNYRTAEILKPDVIAPGVNILAGWSGYSSPTGLNIDPRRVEFN 540

Query: 541 IISGTSMSCPHVSGVAALLRKAFPTWSPAAIKSALITTSYSLDSSGSPIKDLSTSKESNP 600
           IISGTSMSCPHVSGVAALLRKAFPTWSPAAIKSALITTSYSLDSSG PIKDLSTS+ESNP
Sbjct: 541 IISGTSMSCPHVSGVAALLRKAFPTWSPAAIKSALITTSYSLDSSGKPIKDLSTSEESNP 600

Query: 601 FVHGAGHIDPNKALNPGLIYDLTPQDYVSFLCSIGYDSRQIAVFVKDSSYSQLCEHKLSN 660
           FVHGAGHI+PN+ALNPGLIYDLTPQDYVSFLCSIGYDS+QIAVFVK SSY QLCEHKL+N
Sbjct: 601 FVHGAGHINPNQALNPGLIYDLTPQDYVSFLCSIGYDSKQIAVFVKGSSYFQLCEHKLTN 660

Query: 661 PGNLNYPSFSVVFDGEEVVKYTRTVTNVGDETD-VYEVKVESPQGVEISVLPNKLEFNAK 720
           PGNLNYPSFSVVFD EEVVKYTRTVTNVGDET+ VYEVKVE+PQGV ISV+PNKLEFN +
Sbjct: 661 PGNLNYPSFSVVFDEEEVVKYTRTVTNVGDETEVVYEVKVEAPQGVVISVVPNKLEFNKE 720

Query: 721 KTTQSYEITFTKISGFKESASFGSIQWSDGSHNVRSPIAVSFNT-GSIASM 768
           KTTQSYEITFTKI+GFKESASFGSIQW DG H+VRSPIAVSF T GSIASM
Sbjct: 721 KTTQSYEITFTKINGFKESASFGSIQWGDGIHSVRSPIAVSFKTGGSIASM 771

BLAST of ClCG09G001040 vs. TrEMBL
Match: M5XH18_PRUPE (Uncharacterized protein OS=Prunus persica GN=PRUPE_ppa001739mg PE=4 SV=1)

HSP 1 Score: 1082.4 bits (2798), Expect = 0.0e+00
Identity = 541/767 (70.53%), Postives = 631/767 (82.27%), Query Frame = 1

Query: 9   SLFFFFIFCFVPVIFSRSPD---NQETFIVHVSKSEKPSLFSSHRHWHSSILESLSPSPH 68
           SLF F +   +P   S  PD     +TFIVHVSKS+KPSLFSSHR W++SI+++L PSPH
Sbjct: 7   SLFLFLLSLLLPATLSIPPDLSDRPKTFIVHVSKSQKPSLFSSHRSWYTSIIQNL-PSPH 66

Query: 69  PTKLLYNYEHAVNGFSARITAAQAAELRHVPGVISVIPDRIRQLHTTRTPHFLGLADNLG 128
           PTKLLY Y+ +V+GFSA +T++QA +L   P V+SV PD+ RQLHTT TP+FLGLAD+ G
Sbjct: 67  PTKLLYTYDRSVHGFSATLTSSQATQLLSHPSVLSVTPDQPRQLHTTHTPNFLGLADSFG 126

Query: 129 LWPDTNYAEDVIIGVLDTGIWPGRPSFSDEGLSPVPASWKGTCEAGEGFSASTCNRKIIG 188
           LWP+++YA+DV+IGVLDTGIWP RPSFSD G+ PVP  WKGTC     F +S CNRKIIG
Sbjct: 127 LWPNSDYADDVVIGVLDTGIWPERPSFSDSGIGPVPTRWKGTCVTTADFPSSACNRKIIG 186

Query: 189 ARAYFNGYESNLGRSL-RSSDFKSARDSDGHGTHTASTAAGSFVKNASFFQYARGEARGM 248
           ARAYFNGYES++GR +  +++ KS RD++GHGTHTASTAAG+ V NASFF YA+G+ARGM
Sbjct: 187 ARAYFNGYESHIGRLMDETTEAKSPRDTEGHGTHTASTAAGAVVANASFFSYAQGDARGM 246

Query: 249 ASRARIAAYKICWEFGCFDSDILAAMDQAIADGVHIISLSVGSSGRAPAYYRDSIAIGAF 308
           A++ARIA YKICW FGCFDSDILAAMDQAIADGV IISLSVG+SG AP Y RDSIAIGAF
Sbjct: 247 ATKARIAVYKICWSFGCFDSDILAAMDQAIADGVDIISLSVGASGNAPPYDRDSIAIGAF 306

Query: 309 GAMQHGVVVSCSAGNSGPGPYTAVNIAPWILTVGASTIDREFLADVILGDGRVFSGVSLY 368
           GA QHGV+VS SAGNSGP P+TA NIAPWILTVGASTIDREF ADV+LGD RV SGVSLY
Sbjct: 307 GAAQHGVLVSASAGNSGPNPFTATNIAPWILTVGASTIDREFPADVVLGDNRVISGVSLY 366

Query: 369 SGEPLGDSKLPLVYGGDCGSRYCYSGSLDPAKVAGKIVVCDRGGNARVAKGGAVKSAGGL 428
           SGEPL D KLPLVYGGDCGSRYCY G+L P+KV GKIVVCDRGGNARVAKGGAVK AGGL
Sbjct: 367 SGEPLVDYKLPLVYGGDCGSRYCYEGALQPSKVQGKIVVCDRGGNARVAKGGAVKLAGGL 426

Query: 429 GMVLANTEENGEELLADSHLIPGTMVGEIAGNKLRNYIHSDPNPTATIVFRGTVIGNSPS 488
           GM+LANTEE+GEELLAD HLIP T VGEIA N++R YI     PTATIVFRGTVIG+SPS
Sbjct: 427 GMILANTEESGEELLADGHLIPATEVGEIAANQIREYIRLSQYPTATIVFRGTVIGSSPS 486

Query: 489 APRVAAFSSRGPNYRTAEILKPDVIAPGVNILAGWSGYSSPTSLSIDSRRVEFNIISGTS 548
           +P+VAAFSSRGPN  T EILKPDVIAPGVNILAGW+G ++PT L ID RRVEFNIISGTS
Sbjct: 487 SPQVAAFSSRGPNSLTPEILKPDVIAPGVNILAGWTGATAPTDLDIDPRRVEFNIISGTS 546

Query: 549 MSCPHVSGVAALLRKAFPTWSPAAIKSALITTSYSLDSSGSPIKDLSTSKESNPFVHGAG 608
           MSCPHVSG+AALLRKAFP WS AAIKSALITT+Y+LD++G  IKDL T +ES PFVHGAG
Sbjct: 547 MSCPHVSGIAALLRKAFPNWSIAAIKSALITTAYTLDNAGKKIKDLGTGEESTPFVHGAG 606

Query: 609 HIDPNKALNPGLIYDLTPQDYVSFLCSIGYDSRQIAVFVKDSSYSQLC-EHKLSNPGNLN 668
           H+DPN+ALNPGLIYDL   DYV+FLCSIGY  RQIAVFV   + S +C  + L++PG+LN
Sbjct: 607 HVDPNRALNPGLIYDLNVNDYVAFLCSIGYSPRQIAVFVGKPTGSDMCTRNSLASPGDLN 666

Query: 669 YPSFSVVFDGEE-VVKYTRTVTNVGDETD-VYEVKVESPQGVEISVLPNKLEFNAKKTTQ 728
           YPSFSVV   ++ ++KY R  TNVG + D VYEV V +P GVEISV P KL F+A+  TQ
Sbjct: 667 YPSFSVVLSSDQGLIKYKRIATNVGGDADAVYEVTVNAPAGVEISVEPRKLVFSAENQTQ 726

Query: 729 SYEITFTKISGFKESASFGSIQWSDGSHNVRSPIAVSFNTG-SIASM 768
           SYE+TF +  G+     +GSI+W+DG H VRSP+AV +++  S+ASM
Sbjct: 727 SYEVTFKRGVGYDGGERYGSIEWTDGRHLVRSPVAVRWSSARSLASM 772

BLAST of ClCG09G001040 vs. TrEMBL
Match: A0A061GCZ9_THECC (Subtilase family protein OS=Theobroma cacao GN=TCM_029155 PE=4 SV=1)

HSP 1 Score: 1077.0 bits (2784), Expect = 0.0e+00
Identity = 538/760 (70.79%), Postives = 622/760 (81.84%), Query Frame = 1

Query: 9   SLFFFFIFCFVPVIFSRSPDNQETFIVHVSKSEKPSLFSSHRHWHSSILESLSPSPHPTK 68
           S FFF    F+P   S S D  E FI+HVSKS KPSLFSSH HW+SSI+ SL PSPHPTK
Sbjct: 4   SFFFFLSLLFIP-FSSSSSDRPENFIIHVSKSHKPSLFSSHHHWYSSIIHSLPPSPHPTK 63

Query: 69  LLYNYEHAVNGFSARITAAQAAELRHVPGVISVIPDRIRQLHTTRTPHFLGLADNLGLWP 128
           LLY YE A+NGFSAR+TA QA +LR +PG++SVIPD++RQ+HTTRTP FLGL+D +GLW 
Sbjct: 64  LLYTYERAINGFSARLTATQAEKLRELPGILSVIPDQVRQIHTTRTPQFLGLSDGVGLWQ 123

Query: 129 DTNYAEDVIIGVLDTGIWPGRPSFSDEGLSPVPASWKGTCEAGEGFSASTCNRKIIGARA 188
           ++ Y + VIIGVLDTGIWP RPSF D GLSPVP SWKG CE G  F AS C+RKIIGARA
Sbjct: 124 NSYYGDGVIIGVLDTGIWPERPSFKDSGLSPVPDSWKGICETGPDFPASACSRKIIGARA 183

Query: 189 YFNGYESNL-GRSLRSSDFKSARDSDGHGTHTASTAAGSFVKNASFFQYARGEARGMASR 248
           ++ GYES L G    + + KS RD++GHGTHTASTAAGS V NAS F++A GEARGMA++
Sbjct: 184 FYKGYESYLEGPMDETKESKSPRDTEGHGTHTASTAAGSVVSNASLFEFAYGEARGMATK 243

Query: 249 ARIAAYKICWEFGCFDSDILAAMDQAIADGVHIISLSVGSSGRAPAYYRDSIAIGAFGAM 308
           ARIAAYKICW  GCFDSD+LAAMDQAIADGV++ISLSVG++G AP Y  DSIAIGAFGA 
Sbjct: 244 ARIAAYKICWSLGCFDSDLLAAMDQAIADGVNVISLSVGATGYAPQYDHDSIAIGAFGAA 303

Query: 309 QHGVVVSCSAGNSGPGPYTAVNIAPWILTVGASTIDREFLADVILGDGRVFSGVSLYSGE 368
           QHG+VVSCSAGNSGPGPYTAVNIAPWILTVGASTIDREF AD ILGDGR+F GVSLYSG+
Sbjct: 304 QHGIVVSCSAGNSGPGPYTAVNIAPWILTVGASTIDREFPADAILGDGRIFGGVSLYSGD 363

Query: 369 PLGDSKLPLVYGGDCGSRYCYSGSLDPAKVAGKIVVCDRGGNARVAKGGAVKSAGGLGMV 428
           PL D KLPLVY GD G+RYCY GSL P+KV GKIV CDRGGNARV KG AVK AGGLGM+
Sbjct: 364 PLVDIKLPLVYAGDSGNRYCYMGSLSPSKVQGKIVFCDRGGNARVEKGFAVKLAGGLGMI 423

Query: 429 LANTEENGEELLADSHLIPGTMVGEIAGNKLRNYIHSDPNPTATIVFRGTVIGNSPSAPR 488
           LANT E+GEEL+AD+HLIP T VGE AGN++R YI     PTATIVF GTVIG SP AP+
Sbjct: 424 LANTAESGEELIADAHLIPATTVGEKAGNEIRQYIKISQFPTATIVFHGTVIGPSPPAPK 483

Query: 489 VAAFSSRGPNYRTAEILKPDVIAPGVNILAGWSGYSSPTSLSIDSRRVEFNIISGTSMSC 548
           VAAFSSRGPN+ T EILKPDVIAPGVNILAGW+G+  P+ L+ID+RRV FNIISGTSMSC
Sbjct: 484 VAAFSSRGPNHLTPEILKPDVIAPGVNILAGWTGFIGPSQLNIDTRRVNFNIISGTSMSC 543

Query: 549 PHVSGVAALLRKAFPTWSPAAIKSALITTSYSLDSSGSPIKDLSTSKESNPFVHGAGHID 608
           PHVSG+AALL KA+P WSPAAIKSAL+TT+YSLD+SG+ IKDL+T  ES+PFV+GAGH+D
Sbjct: 544 PHVSGLAALLIKAYPNWSPAAIKSALMTTAYSLDNSGNTIKDLATGVESSPFVYGAGHVD 603

Query: 609 PNKALNPGLIYDLTPQDYVSFLCSIGYDSRQIAVFVKDSSYSQLCEHKLSNPGNLNYPSF 668
           PN AL PGL+YD+   DYV+FLCSIGYDS++IA+FV++ +   +CE KL+ PGNLNYPSF
Sbjct: 604 PNIALMPGLVYDIDDGDYVAFLCSIGYDSKRIAIFVREPTGPDVCEGKLATPGNLNYPSF 663

Query: 669 SVVFD-GEEVVKYTRTVTNVGDETD-VYEVKVESPQGVEISVLPNKLEFNAKKTTQSYEI 728
           SVVFD  + VVKY RTV NVG   D VYE KV +P GVEISV P+KLEF+A+  T SYEI
Sbjct: 664 SVVFDSNDHVVKYKRTVKNVGPSVDAVYEAKVNAPPGVEISVSPSKLEFSAENQTLSYEI 723

Query: 729 TFTK--ISGFKES-ASFGSIQWSDGSHNVRSPIAVSFNTG 763
           TF    ++ F  +  +FGSI+WSDG H VRSPIAV +  G
Sbjct: 724 TFASDGLALFAVALEAFGSIEWSDGVHLVRSPIAVRWLQG 762

BLAST of ClCG09G001040 vs. TrEMBL
Match: M1A1D2_SOLTU (Uncharacterized protein OS=Solanum tuberosum GN=PGSC0003DMG400004891 PE=4 SV=1)

HSP 1 Score: 1075.1 bits (2779), Expect = 0.0e+00
Identity = 535/778 (68.77%), Postives = 624/778 (80.21%), Query Frame = 1

Query: 1   MANFHRSSSLFFFFIFCFVPVIFSRSPDNQETFIVHVSKSEKPSLFSSHRHWHSSILESL 60
           MA     SS+FF   FC  PV  S   D  ETFI+HV+KS+KP +FS+H HW+SSI+ S+
Sbjct: 1   MAKISVLSSIFFIISFCLTPVAISVQSDGHETFIIHVAKSDKPHVFSTHHHWYSSIVRSI 60

Query: 61  SP-SPHPTKLLYNYEHAVNGFSARITAAQAAELRHVPGVISVIPDRIRQLHTTRTPHFLG 120
           SP S H +K+LY YE A  GFSAR+TA QA +LR VPGVISVIPD++R LHTT TP FL 
Sbjct: 61  SPPSHHRSKILYTYERAAVGFSARLTAGQADQLRRVPGVISVIPDQVRYLHTTHTPTFLK 120

Query: 121 LADNLGLWPDTNYAEDVIIGVLDTGIWPGRPSFSDEGLSPVPASWKGTCEAGEGFSASTC 180
           LAD+ GLWPD++YA+DVI+GVLDTGIWP RPSFSDEGLSPVPA WKG C  G GF  S+C
Sbjct: 121 LADSFGLWPDSDYADDVIVGVLDTGIWPERPSFSDEGLSPVPAGWKGKCVTGPGFPRSSC 180

Query: 181 NRKIIGARAYFNGYESNLGRSLRSSDFKSARDSDGHGTHTASTAAGSFVKNASFFQYARG 240
           NRKIIGAR ++ GYE++ G    S + KS RD++GHGTHTASTAAGS V NASF+QYA+G
Sbjct: 181 NRKIIGARMFYKGYEASHGPMDESKEAKSPRDTEGHGTHTASTAAGSLVANASFYQYAKG 240

Query: 241 EARGMASRARIAAYKICWEFGCFDSDILAAMDQAIADGVHIISLSVGSSGRAPAYYRDSI 300
           EARGMA +ARIAAYKICW+ GCFDSDILAAMDQA+ADGVH+ISLSVG++G AP Y  DSI
Sbjct: 241 EARGMAIKARIAAYKICWKDGCFDSDILAAMDQAVADGVHVISLSVGANGYAPHYLHDSI 300

Query: 301 AIGAFGAMQHGVVVSCSAGNSGPGPYTAVNIAPWILTVGASTIDREFLADVILGDGRVFS 360
           AIGAFGA +HGV+VSCSAGNSGPGPYTAVNIAPWILTVGASTIDREF ADVILGD RVF 
Sbjct: 301 AIGAFGASEHGVLVSCSAGNSGPGPYTAVNIAPWILTVGASTIDREFPADVILGDDRVFG 360

Query: 361 GVSLYSGEPLGDSKLPLVYGGDCGSRYCYSGSLDPAKVAGKIVVCDRGGNARVAKGGAVK 420
           GVSLYSG PL DSK P+VY GDCGS+YCY G LD  KVAGKIV+CDRGGNARV KG AVK
Sbjct: 361 GVSLYSGNPLTDSKFPVVYSGDCGSKYCYPGKLDHKKVAGKIVLCDRGGNARVEKGSAVK 420

Query: 421 SAGGLGMVLANTEENGEELLADSHLIPGTMVGEIAGNKLRNYIHSDPNPTATIVFRGTVI 480
            AGG+GM+LAN  E+GEEL+ADSHL+P TMVG+ AG+K+R Y+ SD +PTATIVFRGTVI
Sbjct: 421 LAGGVGMILANLAESGEELVADSHLLPATMVGQKAGDKIREYVTSDTSPTATIVFRGTVI 480

Query: 481 GNSPSAPRVAAFSSRGPNYRTAEILKPDVIAPGVNILAGWSGYSSPTSLSIDSRRVEFNI 540
           GNSP+APRVAAFSSRGPN+ T EILKPDVIAPGVNILAGW+G + PT L+ID RRVEFNI
Sbjct: 481 GNSPAAPRVAAFSSRGPNHLTPEILKPDVIAPGVNILAGWTGSTGPTDLAIDPRRVEFNI 540

Query: 541 ISGTSMSCPHVSGVAALLRKAFPTWSPAAIKSALITTSYSLDSSGSPIKDLSTSKESNPF 600
           ISGTSMSCPHVSG+AALLR+A   W+PAAIKSAL+TT+Y+LD+SG    DL+T +ES PF
Sbjct: 541 ISGTSMSCPHVSGLAALLRRAHSKWTPAAIKSALMTTAYNLDNSGKIFTDLATGEESTPF 600

Query: 601 VHGAGHIDPNKALNPGLIYDLTPQDYVSFLCSIGYDSRQIAVFVKDSSYSQLCEHKLSNP 660
           VHG+GH+DPN+AL+PGL+YD+   DYV+FLC+IGYD   IAVFV+DSS     E  L+ P
Sbjct: 601 VHGSGHVDPNRALDPGLVYDIETSDYVNFLCTIGYDGDDIAVFVRDSSRVNCSERSLATP 660

Query: 661 GNLNYPSFSVVF--DGEEVVKYTRTVTNV-GDETDVYEVKVESPQGVEISVLPNKLEFNA 720
           G+LNYPSFSV F  D   VVKY R V NV GD   VYEVKV +P  VE+SV P KL F+ 
Sbjct: 661 GDLNYPSFSVDFTSDSNGVVKYKRVVKNVGGDSNAVYEVKVNAPSAVEVSVSPAKLVFSE 720

Query: 721 KKTTQSYEITFTK-------ISGFKESASFGSIQWSDGSHNVRSPIAVSFNTGSIASM 768
           +  + SYEI+FT        + G +  ++FGSI+WSDG H+VRSPIAV +   S  SM
Sbjct: 721 ENNSLSYEISFTSKRSEDIMVKGIQ--SAFGSIEWSDGIHSVRSPIAVRWRYQSAVSM 776

BLAST of ClCG09G001040 vs. TrEMBL
Match: A0A059AIP6_EUCGR (Uncharacterized protein OS=Eucalyptus grandis GN=EUGRSUZ_J02805 PE=4 SV=1)

HSP 1 Score: 1072.8 bits (2773), Expect = 1.7e-310
Identity = 525/771 (68.09%), Postives = 626/771 (81.19%), Query Frame = 1

Query: 7   SSSLFFFFIFCFVPVIF----SRSPDNQ---ETFIVHVSKSEKPSLFSSHRHWHSSILES 66
           SSS  F  IF F+ ++F    + +P  Q   +TFI+H S S KPSLFSSH HW+SS+L S
Sbjct: 8   SSSSSFLLIFIFILLLFISPSASTPQEQSHHQTFIIHASSSHKPSLFSSHHHWYSSLLHS 67

Query: 67  LSPSPHPTKLLYNYEHAVNGFSARITAAQAAELRHVPGVISVIPDRIRQLHTTRTPHFLG 126
           L PS  P+++LY Y  A  GFSAR+TA QA  LR  PGV+SV+PDRIR LHTTRTP FLG
Sbjct: 68  LPPSHPPSQILYTYRAAAAGFSARLTAPQAEALRRAPGVLSVVPDRIRHLHTTRTPRFLG 127

Query: 127 LADNLGLWPDTNYAEDVIIGVLDTGIWPGRPSFSDEGLSPVPASWKGTCEAGEGFSASTC 186
           L ++ GLWP+++YA+D+IIGVLDTGIWP R SFSD GL PVP +W+GTCE G  F AS C
Sbjct: 128 LTESFGLWPNSDYADDIIIGVLDTGIWPERRSFSDSGLGPVPGAWRGTCEVGRDFPASAC 187

Query: 187 NRKIIGARAYFNGYESNLGRSL-RSSDFKSARDSDGHGTHTASTAAGSFVKNASFFQYAR 246
           NRKIIGARA++ GYE+ L R +  + + KS RD++GHGTHT+STA GS V NASF++YA 
Sbjct: 188 NRKIIGARAFYKGYEAYLERPIDETKESKSPRDTEGHGTHTSSTAGGSVVSNASFYEYAV 247

Query: 247 GEARGMASRARIAAYKICWEFGCFDSDILAAMDQAIADGVHIISLSVGSSGRAPAYYRDS 306
           GEARGMA++ARIAAYKICW  GCFDSDILAAMDQA++DGVHIISLSVG++G AP Y  DS
Sbjct: 248 GEARGMATKARIAAYKICWSLGCFDSDILAAMDQAVSDGVHIISLSVGANGYAPQYDHDS 307

Query: 307 IAIGAFGAMQHGVVVSCSAGNSGPGPYTAVNIAPWILTVGASTIDREFLADVILGDGRVF 366
           IAIGAFGA++HGV+VSCSAGNSGPGP+TAVNIAPWILTVGASTIDREF ADV+LGDGR+F
Sbjct: 308 IAIGAFGAVKHGVLVSCSAGNSGPGPFTAVNIAPWILTVGASTIDREFPADVVLGDGRIF 367

Query: 367 SGVSLYSGEPLGDSKLPLVYGGDCGSRYCYSGSLDPAKVAGKIVVCDRGGNARVAKGGAV 426
            GVSLYSGEPLGD+KLPLVY GDCGSRYCY  SLD  KVAGKIVVCDRGGNARVAKG AV
Sbjct: 368 KGVSLYSGEPLGDAKLPLVYAGDCGSRYCYDDSLDSTKVAGKIVVCDRGGNARVAKGSAV 427

Query: 427 KSAGGLGMVLANTEENGEELLADSHLIPGTMVGEIAGNKLRNYIHSDPNPTATIVFRGTV 486
           K AGG+GM+LANTEE+GEEL+ADSHLIP  MVGE AG+K+R Y  SD  PTA I F GTV
Sbjct: 428 KLAGGVGMILANTEESGEELIADSHLIPANMVGETAGDKIREYASSDQFPTAAIKFIGTV 487

Query: 487 IGNSPSAPRVAAFSSRGPNYRTAEILKPDVIAPGVNILAGWSGYSSPTSLSIDSRRVEFN 546
           IG SP AP+VAAFSSRGPN+ TAEILKPDVIAPGVNILA W+G++ PT L ID RRVEFN
Sbjct: 488 IGPSPPAPKVAAFSSRGPNHLTAEILKPDVIAPGVNILAAWTGFNGPTDLDIDPRRVEFN 547

Query: 547 IISGTSMSCPHVSGVAALLRKAFPTWSPAAIKSALITTSYSLDSSGSPIKDLSTSKESNP 606
           IISGTSMSCPH SG+AALLRKA+P WSPAAIKSAL+TT+Y+LD+ G  IKDL++  ES+P
Sbjct: 548 IISGTSMSCPHASGLAALLRKAYPDWSPAAIKSALMTTAYNLDNGGRNIKDLASGDESSP 607

Query: 607 FVHGAGHIDPNKALNPGLIYDLTPQDYVSFLCSIGYDSRQIAVFVKDSSYSQLCEHKLSN 666
           FVHGAGH+DPN+ALNPGL+YD+   DYV FLC+IGY+ R+IAVF+++   S +C ++++ 
Sbjct: 608 FVHGAGHVDPNRALNPGLVYDIDVDDYVGFLCAIGYEPRRIAVFLREPMSSTVCANRMAT 667

Query: 667 PGNLNYPSFSVVFDGE-EVVKYTRTVTNVGDETD-VYEVKVESPQGVEISVLPNKLEFNA 726
           PG LNYPSFSVV     +VVKY R V NVG   D VYEVKV++P+ VE+SV P+KLEF+A
Sbjct: 668 PGELNYPSFSVVLTSNGDVVKYRRVVKNVGSSADAVYEVKVDAPENVEVSVSPSKLEFSA 727

Query: 727 KKTTQSYEITFTKISGFKESASFGSIQWSDGSHNVRSPIAVSFNTGSIASM 768
           +    SYEITF+ I+    +  FGSI+W+DG+H VRSPIAV ++   + S+
Sbjct: 728 ENQILSYEITFSSIALGSSTPKFGSIEWTDGTHRVRSPIAVKWHQELVESI 778

BLAST of ClCG09G001040 vs. TAIR10
Match: AT3G14067.1 (AT3G14067.1 Subtilase family protein)

HSP 1 Score: 988.0 bits (2553), Expect = 3.2e-288
Identity = 493/771 (63.94%), Postives = 590/771 (76.52%), Query Frame = 1

Query: 8   SSLFFFF--IFCFVPVIFSRSPDNQETFIVHVSKSEKPSLFSSHRHWHSSILESLSPSPH 67
           SS+FF F  + CF     S S D  E++IVHV +S KPSLFSSH +WH S+L SL  SP 
Sbjct: 7   SSIFFVFPLLLCFFSPS-SSSSDGLESYIVHVQRSHKPSLFSSHNNWHVSLLRSLPSSPQ 66

Query: 68  PTKLLYNYEHAVNGFSARITAAQAAELRHVPGVISVIPDRIRQLHTTRTPHFLGLADNLG 127
           P  LLY+Y  AV+GFSAR++  Q A LR  P VISVIPD+ R++HTT TP FLG + N G
Sbjct: 67  PATLLYSYSRAVHGFSARLSPIQTAALRRHPSVISVIPDQAREIHTTHTPAFLGFSQNSG 126

Query: 128 LWPDTNYAEDVIIGVLDTGIWPGRPSFSDEGLSPVPASWKGTCEAGEGFSASTCNRKIIG 187
           LW ++NY EDVI+GVLDTGIWP  PSFSD GL P+P++WKG CE G  F AS+CNRK+IG
Sbjct: 127 LWSNSNYGEDVIVGVLDTGIWPEHPSFSDSGLGPIPSTWKGECEIGPDFPASSCNRKLIG 186

Query: 188 ARAYFNGY--ESNLGRSLRSSDFKSARDSDGHGTHTASTAAGSFVKNASFFQYARGEARG 247
           ARA++ GY  + N  +   + + +S RD++GHGTHTASTAAGS V NAS +QYARG A G
Sbjct: 187 ARAFYRGYLTQRNGTKKHAAKESRSPRDTEGHGTHTASTAAGSVVANASLYQYARGTATG 246

Query: 248 MASRARIAAYKICWEFGCFDSDILAAMDQAIADGVHIISLSVGSSGRAPAYYRDSIAIGA 307
           MAS+ARIAAYKICW  GC+DSDILAAMDQA+ADGVH+ISLSVG+SG AP Y+ DSIAIGA
Sbjct: 247 MASKARIAAYKICWTGGCYDSDILAAMDQAVADGVHVISLSVGASGSAPEYHTDSIAIGA 306

Query: 308 FGAMQHGVVVSCSAGNSGPGPYTAVNIAPWILTVGASTIDREFLADVILGDGRVFSGVSL 367
           FGA +HG+VVSCSAGNSGP P TA NIAPWILTVGAST+DREF A+ I GDG+VF+G SL
Sbjct: 307 FGATRHGIVVSCSAGNSGPNPETATNIAPWILTVGASTVDREFAANAITGDGKVFTGTSL 366

Query: 368 YSGEPLGDSKLPLVYGGDCGSRYCYSGSLDPAKVAGKIVVCDRGGNARVAKGGAVKSAGG 427
           Y+GE L DS+L LVY GDCGSR CY G L+ + V GKIV+CDRGGNARV KG AVK AGG
Sbjct: 367 YAGESLPDSQLSLVYSGDCGSRLCYPGKLNSSLVEGKIVLCDRGGNARVEKGSAVKLAGG 426

Query: 428 LGMVLANTEENGEELLADSHLIPGTMVGEIAGNKLRNYIHSDPNPTATIVFRGTVIGNSP 487
            GM+LANT E+GEEL ADSHL+P TMVG  AG+++R+YI +  +PTA I F GT+IG SP
Sbjct: 427 AGMILANTAESGEELTADSHLVPATMVGAKAGDQIRDYIKTSDSPTAKISFLGTLIGPSP 486

Query: 488 SAPRVAAFSSRGPNYRTAEILKPDVIAPGVNILAGWSGYSSPTSLSIDSRRVEFNIISGT 547
            +PRVAAFSSRGPN+ T  ILKPDVIAPGVNILAGW+G   PT L ID RRV+FNIISGT
Sbjct: 487 PSPRVAAFSSRGPNHLTPVILKPDVIAPGVNILAGWTGMVGPTDLDIDPRRVQFNIISGT 546

Query: 548 SMSCPHVSGVAALLRKAFPTWSPAAIKSALITTSYSLDSSGSPIKDLSTSKESNPFVHGA 607
           SMSCPHVSG+AALLRKA P WSPAAIKSAL+TT+Y +++SG PI+DL+T K SN F+HGA
Sbjct: 547 SMSCPHVSGLAALLRKAHPDWSPAAIKSALVTTAYDVENSGEPIEDLATGKSSNSFIHGA 606

Query: 608 GHIDPNKALNPGLIYDLTPQDYVSFLCSIGYDSRQIAVFVKDSSYSQLCE-HKLSNPGNL 667
           GH+DPNKALNPGL+YD+  ++YV+FLC++GY+   I VF++D +    CE  KL   G+L
Sbjct: 607 GHVDPNKALNPGLVYDIEVKEYVAFLCAVGYEFPGILVFLQDPTLYDACETSKLRTAGDL 666

Query: 668 NYPSFSVVFDGE-EVVKYTRTVTNVGDETD-VYEVKVESPQGVEISVLPNKLEFNAKKTT 727
           NYPSFSVVF    EVVKY R V NVG   D VYEV V+SP  VEI V P+KL F+ +K+ 
Sbjct: 667 NYPSFSVVFASTGEVVKYKRVVKNVGSNVDAVYEVGVKSPANVEIDVSPSKLAFSKEKSV 726

Query: 728 QSYEITFTKI-----SGFKESASFGSIQWSDGSHNVRSPIAVSFNTGSIAS 767
             YE+TF  +      G      FGSI+W+DG H V+SP+AV +  GS+ S
Sbjct: 727 LEYEVTFKSVVLGGGVGSVPGHEFGSIEWTDGEHVVKSPVAVQWGQGSVQS 776

BLAST of ClCG09G001040 vs. TAIR10
Match: AT5G67360.1 (AT5G67360.1 Subtilase family protein)

HSP 1 Score: 815.1 bits (2104), Expect = 3.7e-236
Identity = 426/760 (56.05%), Postives = 531/760 (69.87%), Query Frame = 1

Query: 8   SSLFFFFIFCFVPVIFSRSPDNQETFIVHVSKSEKPSLFSSHRHWHSSILESLSPSPHPT 67
           SS  FF + C      S S  +Q T+IVH++KS+ PS F  H +W+ S L S+S S    
Sbjct: 7   SSTAFFLLLCLGFCHVSSSSSDQGTYIVHMAKSQMPSSFDLHSNWYDSSLRSISDS---A 66

Query: 68  KLLYNYEHAVNGFSARITAAQAAELRHVPGVISVIPDRIRQLHTTRTPHFLGLADNLG-L 127
           +LLY YE+A++GFS R+T  +A  L   PGVISV+P+   +LHTTRTP FLGL ++   L
Sbjct: 67  ELLYTYENAIHGFSTRLTQEEADSLMTQPGVISVLPEHRYELHTTRTPLFLGLDEHTADL 126

Query: 128 WPDTNYAEDVIIGVLDTGIWPGRPSFSDEGLSPVPASWKGTCEAGEGFSASTCNRKIIGA 187
           +P+     DV++GVLDTG+WP   S+SDEG  P+P+SWKG CEAG  F+AS CNRK+IGA
Sbjct: 127 FPEAGSYSDVVVGVLDTGVWPESKSYSDEGFGPIPSSWKGGCEAGTNFTASLCNRKLIGA 186

Query: 188 RAYFNGYESNLGRSLRSSDFKSARDSDGHGTHTASTAAGSFVKNASFFQYARGEARGMAS 247
           R +  GYES +G    S + +S RD DGHGTHT+STAAGS V+ AS   YA G ARGMA 
Sbjct: 187 RFFARGYESTMGPIDESKESRSPRDDDGHGTHTSSTAAGSVVEGASLLGYASGTARGMAP 246

Query: 248 RARIAAYKICWEFGCFDSDILAAMDQAIADGVHIISLSVGSSGRAPAYYRDSIAIGAFGA 307
           RAR+A YK+CW  GCF SDILAA+D+AIAD V+++S+S+G  G    YYRD +AIGAF A
Sbjct: 247 RARVAVYKVCWLGGCFSSDILAAIDKAIADNVNVLSMSLG--GGMSDYYRDGVAIGAFAA 306

Query: 308 MQHGVVVSCSAGNSGPGPYTAVNIAPWILTVGASTIDREFLADVILGDGRVFSGVSLYSG 367
           M+ G++VSCSAGN+GP   +  N+APWI TVGA T+DR+F A  ILG+G+ F+GVSL+ G
Sbjct: 307 MERGILVSCSAGNAGPSSSSLSNVAPWITTVGAGTLDRDFPALAILGNGKNFTGVSLFKG 366

Query: 368 EPLGDSKLPLVYGGDCGS----RYCYSGSLDPAKVAGKIVVCDRGGNARVAKGGAVKSAG 427
           E L D  LP +Y G+  +      C +G+L P KV GKIV+CDRG NARV KG  VK+AG
Sbjct: 367 EALPDKLLPFIYAGNASNATNGNLCMTGTLIPEKVKGKIVMCDRGINARVQKGDVVKAAG 426

Query: 428 GLGMVLANTEENGEELLADSHLIPGTMVGEIAGNKLRNYIHSDPNPTATIVFRGTVIGNS 487
           G+GM+LANT  NGEEL+AD+HL+P T VGE AG+ +R+Y+ +DPNPTA+I   GTV+G  
Sbjct: 427 GVGMILANTAANGEELVADAHLLPATTVGEKAGDIIRHYVTTDPNPTASISILGTVVGVK 486

Query: 488 PSAPRVAAFSSRGPNYRTAEILKPDVIAPGVNILAGWSGYSSPTSLSIDSRRVEFNIISG 547
           PS P VAAFSSRGPN  T  ILKPD+IAPGVNILA W+G + PT L+ DSRRVEFNIISG
Sbjct: 487 PS-PVVAAFSSRGPNSITPNILKPDLIAPGVNILAAWTGAAGPTGLASDSRRVEFNIISG 546

Query: 548 TSMSCPHVSGVAALLRKAFPTWSPAAIKSALITTSYSLDSSGSPIKDLSTSKESNPFVHG 607
           TSMSCPHVSG+AALL+   P WSPAAI+SAL+TT+Y     G P+ D++T K S PF HG
Sbjct: 547 TSMSCPHVSGLAALLKSVHPEWSPAAIRSALMTTAYKTYKDGKPLLDIATGKPSTPFDHG 606

Query: 608 AGHIDPNKALNPGLIYDLTPQDYVSFLCSIGYDSRQIAVFVKDSSYSQLCE-HKLSNPGN 667
           AGH+ P  A NPGLIYDLT +DY+ FLC++ Y S QI      S  +  C+  K  +  +
Sbjct: 607 AGHVSPTTATNPGLIYDLTTEDYLGFLCALNYTSPQIR---SVSRRNYTCDPSKSYSVAD 666

Query: 668 LNYPSFSVVFDGEEVVKYTRTVTNVGDETDVYEVKVES-PQGVEISVLPNKLEFNAKKTT 727
           LNYPSF+V  DG    KYTRTVT+VG     Y VKV S   GV+ISV P  L F      
Sbjct: 667 LNYPSFAVNVDGVGAYKYTRTVTSVGG-AGTYSVKVTSETTGVKISVEPAVLNFKEANEK 726

Query: 728 QSYEITFTKISGFKE-SASFGSIQWSDGSHNVRSPIAVSF 760
           +SY +TFT  S     S SFGSI+WSDG H V SP+A+S+
Sbjct: 727 KSYTVTFTVDSSKPSGSNSFGSIEWSDGKHVVGSPVAISW 756

BLAST of ClCG09G001040 vs. TAIR10
Match: AT2G05920.1 (AT2G05920.1 Subtilase family protein)

HSP 1 Score: 765.8 bits (1976), Expect = 2.6e-221
Identity = 395/737 (53.60%), Postives = 501/737 (67.98%), Query Frame = 1

Query: 30  QETFIVHVSKSEKPSLFSSHRHWHSSILESLSPSPHPTKLLYNYEHAVNGFSARITAAQA 89
           ++T+I+ V+ S+KP  F +H  W++S L S S       LLY Y  + +GFSA + + +A
Sbjct: 27  KKTYIIRVNHSDKPESFLTHHDWYTSQLNSES------SLLYTYTTSFHGFSAYLDSTEA 86

Query: 90  AELRHVPG-VISVIPDRIRQLHTTRTPHFLGLADNLGLWPDTNYAEDVIIGVLDTGIWPG 149
             L      ++ +  D +  LHTTRTP FLGL    G+    + +  VIIGVLDTG+WP 
Sbjct: 87  DSLLSSSNSILDIFEDPLYTLHTTRTPEFLGLNSEFGVHDLGSSSNGVIIGVLDTGVWPE 146

Query: 150 RPSFSDEGLSPVPASWKGTCEAGEGFSASTCNRKIIGARAYFNGYESNLGRSLRSS-DFK 209
             SF D  +  +P+ WKG CE+G  F +  CN+K+IGAR++  G++   G    S  +  
Sbjct: 147 SRSFDDTDMPEIPSKWKGECESGSDFDSKLCNKKLIGARSFSKGFQMASGGGFSSKRESV 206

Query: 210 SARDSDGHGTHTASTAAGSFVKNASFFQYARGEARGMASRARIAAYKICWEFGCFDSDIL 269
           S RD DGHGTHT++TAAGS V+NASF  YA G ARGMA+RAR+A YK+CW  GCF SDIL
Sbjct: 207 SPRDVDGHGTHTSTTAAGSAVRNASFLGYAAGTARGMATRARVATYKVCWSTGCFGSDIL 266

Query: 270 AAMDQAIADGVHIISLSVGSSGRAPAYYRDSIAIGAFGAMQHGVVVSCSAGNSGPGPYTA 329
           AAMD+AI DGV ++SLS+G  G AP YYRD+IAIGAF AM+ GV VSCSAGNSGP   + 
Sbjct: 267 AAMDRAILDGVDVLSLSLGG-GSAP-YYRDTIAIGAFSAMERGVFVSCSAGNSGPTRASV 326

Query: 330 VNIAPWILTVGASTIDREFLADVILGDGRVFSGVSLYSGEPLGDSKLPLVY--GGDCGSR 389
            N+APW++TVGA T+DR+F A   LG+G+  +GVSLYSG  +G   L LVY  G    S 
Sbjct: 327 ANVAPWVMTVGAGTLDRDFPAFANLGNGKRLTGVSLYSGVGMGTKPLELVYNKGNSSSSN 386

Query: 390 YCYSGSLDPAKVAGKIVVCDRGGNARVAKGGAVKSAGGLGMVLANTEENGEELLADSHLI 449
            C  GSLD + V GKIVVCDRG NARV KG  V+ AGGLGM++ANT  +GEEL+ADSHL+
Sbjct: 387 LCLPGSLDSSIVRGKIVVCDRGVNARVEKGAVVRDAGGLGMIMANTAASGEELVADSHLL 446

Query: 450 PGTMVGEIAGNKLRNYIHSDPNPTATIVFRGTVIGNSPSAPRVAAFSSRGPNYRTAEILK 509
           P   VG+  G+ LR Y+ SD  PTA +VF+GTV+   PS P VAAFSSRGPN  T EILK
Sbjct: 447 PAIAVGKKTGDLLREYVKSDSKPTALLVFKGTVLDVKPS-PVVAAFSSRGPNTVTPEILK 506

Query: 510 PDVIAPGVNILAGWSGYSSPTSLSIDSRRVEFNIISGTSMSCPHVSGVAALLRKAFPTWS 569
           PDVI PGVNILAGWS    PT L  DSRR +FNI+SGTSMSCPH+SG+A LL+ A P WS
Sbjct: 507 PDVIGPGVNILAGWSDAIGPTGLDKDSRRTQFNIMSGTSMSCPHISGLAGLLKAAHPEWS 566

Query: 570 PAAIKSALITTSYSLDSSGSPIKDLSTSKESNPFVHGAGHIDPNKALNPGLIYDLTPQDY 629
           P+AIKSAL+TT+Y LD++ +P+ D + +  SNP+ HG+GH+DP KAL+PGL+YD++ ++Y
Sbjct: 567 PSAIKSALMTTAYVLDNTNAPLHDAADNSLSNPYAHGSGHVDPQKALSPGLVYDISTEEY 626

Query: 630 VSFLCSIGYDSRQIAVFVKDSSYSQLCEHKLSNPGNLNYPSFSVVFDGEEVVKYTRTVTN 689
           + FLCS+ Y    I   VK  S +  C  K S+PG LNYPSFSV+F G+ VV+YTR VTN
Sbjct: 627 IRFLCSLDYTVDHIVAIVKRPSVN--CSKKFSDPGQLNYPSFSVLFGGKRVVRYTREVTN 686

Query: 690 VGDETDVYEVKVESPQGVEISVLPNKLEFNAKKTTQSYEITFTKISG--FKESASFGSIQ 749
           VG  + VY+V V     V ISV P+KL F +    + Y +TF    G      A FGSI 
Sbjct: 687 VGAASSVYKVTVNGAPSVGISVKPSKLSFKSVGEKKRYTVTFVSKKGVSMTNKAEFGSIT 746

Query: 750 WSDGSHNVRSPIAVSFN 761
           WS+  H VRSP+A S+N
Sbjct: 747 WSNPQHEVRSPVAFSWN 752

BLAST of ClCG09G001040 vs. TAIR10
Match: AT3G14240.1 (AT3G14240.1 Subtilase family protein)

HSP 1 Score: 758.8 bits (1958), Expect = 3.2e-219
Identity = 395/773 (51.10%), Postives = 514/773 (66.49%), Query Frame = 1

Query: 11  FFFFIFCFVPVIFSRSPDNQETFIVHVSKSEKPSLFSSHRHWHSSILESLSPSPHPTKLL 70
           +FFF+        S S  N  T+IVHV    KPS+F +H HW++S L SL+ SP    ++
Sbjct: 6   YFFFLLTLSSPSSSASSSNSLTYIVHVDHEAKPSIFPTHFHWYTSSLASLTSSP--PSII 65

Query: 71  YNYEHAVNGFSARITAAQAAELRHVPGVISVIPDRIRQLHTTRTPHFLGL--ADNLGLWP 130
           + Y+   +GFSAR+T+  A++L   P VISVIP+++R LHTTR+P FLGL   D  GL  
Sbjct: 66  HTYDTVFHGFSARLTSQDASQLLDHPHVISVIPEQVRHLHTTRSPEFLGLRSTDKAGLLE 125

Query: 131 DTNYAEDVIIGVLDTGIWPGRPSFSDEGLSPVPASWKGTCEAGEGFSASTCNRKIIGARA 190
           ++++  D++IGV+DTG+WP RPSF D GL PVP  WKG C A + F  S CNRK++GAR 
Sbjct: 126 ESDFGSDLVIGVIDTGVWPERPSFDDRGLGPVPIKWKGQCIASQDFPESACNRKLVGARF 185

Query: 191 YFNGYESNLGRSLRSSDFKSARDSDGHGTHTASTAAGSFVKNASFFQYARGEARGMASRA 250
           +  GYE+  G+   +++F+S RDSDGHGTHTAS +AG +V  AS   YA G A GMA +A
Sbjct: 186 FCGGYEATNGKMNETTEFRSPRDSDGHGTHTASISAGRYVFPASTLGYAHGVAAGMAPKA 245

Query: 251 RIAAYKICWEFGCFDSDILAAMDQAIADGVHIISLSVGSSGRAPAYYRDSIAIGAFGAMQ 310
           R+AAYK+CW  GC+DSDILAA D A+ADGV +ISLSVG  G    YY D+IAIGAFGA+ 
Sbjct: 246 RLAAYKVCWNSGCYDSDILAAFDTAVADGVDVISLSVG--GVVVPYYLDAIAIGAFGAID 305

Query: 311 HGVVVSCSAGNSGPGPYTAVNIAPWILTVGASTIDREFLADVILGDGRVFSGVSLYSGEP 370
            G+ VS SAGN GPG  T  N+APW+ TVGA TIDR+F A+V LG+G++ SGVS+Y G  
Sbjct: 306 RGIFVSASAGNGGPGALTVTNVAPWMTTVGAGTIDRDFPANVKLGNGKMISGVSVYGGPG 365

Query: 371 LGDSKL-PLVYGGDC------GSRYCYSGSLDPAKVAGKIVVCDRGGNARVAKGGAVKSA 430
           L   ++ PLVYGG         S  C  GSLDP  V GKIV+CDRG N+R  KG  V+  
Sbjct: 366 LDPGRMYPLVYGGSLLGGDGYSSSLCLEGSLDPNLVKGKIVLCDRGINSRATKGEIVRKN 425

Query: 431 GGLGMVLANTEENGEELLADSHLIPGTMVGEIAGNKLRNYI------HSDPNPTATIVFR 490
           GGLGM++AN   +GE L+AD H++P T VG   G+++R YI       S  +PTATIVF+
Sbjct: 426 GGLGMIIANGVFDGEGLVADCHVLPATSVGASGGDEIRRYISESSKSRSSKHPTATIVFK 485

Query: 491 GTVIGNSPSAPRVAAFSSRGPNYRTAEILKPDVIAPGVNILAGWSGYSSPTSLSIDSRRV 550
           GT +G  P AP VA+FS+RGPN  T EILKPDVIAPG+NILA W     P+ ++ D+RR 
Sbjct: 486 GTRLGIRP-APVVASFSARGPNPETPEILKPDVIAPGLNILAAWPDRIGPSGVTSDNRRT 545

Query: 551 EFNIISGTSMSCPHVSGVAALLRKAFPTWSPAAIKSALITTSYSLDSSGSPIKDLSTSKE 610
           EFNI+SGTSM+CPHVSG+AALL+ A P WSPAAI+SALITT+Y++D+SG P+ D ST   
Sbjct: 546 EFNILSGTSMACPHVSGLAALLKAAHPDWSPAAIRSALITTAYTVDNSGEPMMDESTGNT 605

Query: 611 SNPFVHGAGHIDPNKALNPGLIYDLTPQDYVSFLCSIGYDSRQIAVFVKDSSYSQLCE-- 670
           S+   +G+GH+ P KA++PGL+YD+T  DY++FLC+  Y    I    +  +    C+  
Sbjct: 606 SSVMDYGSGHVHPTKAMDPGLVYDITSYDYINFLCNSNYTRTNIVTITRRQAD---CDGA 665

Query: 671 HKLSNPGNLNYPSFSVVFD--GEEVVK--YTRTVTNVGDETDVYEVKVESPQGVEISVLP 730
            +  + GNLNYPSFSVVF   GE  +   + RTVTNVGD   VYE+K+  P+G  ++V P
Sbjct: 666 RRAGHVGNLNYPSFSVVFQQYGESKMSTHFIRTVTNVGDSDSVYEIKIRPPRGTTVTVEP 725

Query: 731 NKLEFNAKKTTQSYEI----TFTKISGFKESASFGSIQWSDGSHNVRSPIAVS 759
            KL F       S+ +    T  K+S    +   G I WSDG  NV SP+ V+
Sbjct: 726 EKLSFRRVGQKLSFVVRVKTTEVKLSPGATNVETGHIVWSDGKRNVTSPLVVT 770

BLAST of ClCG09G001040 vs. TAIR10
Match: AT5G51750.1 (AT5G51750.1 subtilase 1.3)

HSP 1 Score: 726.1 bits (1873), Expect = 2.3e-209
Identity = 378/772 (48.96%), Postives = 505/772 (65.41%), Query Frame = 1

Query: 12  FFFIFCFVPVIFSRSP-----DNQETFIVHVSKSEKPSLFSSHRHWHSSILESLSPSP-- 71
           F FI   + +IF ++        ++T+++H+ KS  P  +++H  W+SS + S++     
Sbjct: 11  FLFIILSINLIFLQAETTTQISTKKTYVIHMDKSAMPLPYTNHLQWYSSKINSVTQHKSQ 70

Query: 72  ----HPTKLLYNYEHAVNGFSARITAAQAAELRHVPGVISVIPDRIRQLHTTRTPHFLGL 131
               +  ++LY Y+ A +G +A++T  +A  L    GV++VIP+   +LHTTR+P FLGL
Sbjct: 71  EEEGNNNRILYTYQTAFHGLAAQLTQEEAERLEEEDGVVAVIPETRYELHTTRSPTFLGL 130

Query: 132 A--DNLGLWPDTNYAEDVIIGVLDTGIWPGRPSFSDEGLSPVPASWKGTCEAGEGFSAST 191
              ++  +W +     DV++GVLDTGIWP   SF+D G+SPVPA+W+G CE G+ F    
Sbjct: 131 ERQESERVWAERVTDHDVVVGVLDTGIWPESESFNDTGMSPVPATWRGACETGKRFLKRN 190

Query: 192 CNRKIIGARAYFNGYESNLGRSLRSSDFKSARDSDGHGTHTASTAAGSFVKNASFFQYAR 251
           CNRKI+GAR ++ GYE+  G+     ++KS RD DGHGTHTA+T AGS VK A+ F +A 
Sbjct: 191 CNRKIVGARVFYRGYEAATGKIDEELEYKSPRDRDGHGTHTAATVAGSPVKGANLFGFAY 250

Query: 252 GEARGMASRARIAAYKICWEFGCFDSDILAAMDQAIADGVHIISLSVGSSGRAPAYYRDS 311
           G ARGMA +AR+AAYK+CW  GCF SDIL+A+DQA+ADGV ++S+S+G  G    Y RDS
Sbjct: 251 GTARGMAQKARVAAYKVCWVGGCFSSDILSAVDQAVADGVQVLSISLG--GGVSTYSRDS 310

Query: 312 IAIGAFGAMQHGVVVSCSAGNSGPGPYTAVNIAPWILTVGASTIDREFLADVILGDGRVF 371
           ++I  FGAM+ GV VSCSAGN GP P +  N++PWI TVGAST+DR+F A V +G  R F
Sbjct: 311 LSIATFGAMEMGVFVSCSAGNGGPDPISLTNVSPWITTVGASTMDRDFPATVKIGTMRTF 370

Query: 372 SGVSLYSGEPL--GDSKLPLVYGGDCGSR-----YCYSGSLDPAKVAGKIVVCDRGGNAR 431
            GVSLY G  +   + + PLVY G   S      +C  G+LD   VAGKIV+CDRG   R
Sbjct: 371 KGVSLYKGRTVLPKNKQYPLVYLGRNASSPDPTSFCLDGALDRRHVAGKIVICDRGVTPR 430

Query: 432 VAKGGAVKSAGGLGMVLANTEENGEELLADSHLIPGTMVGEIAGNKLRNYIHSDPNPTAT 491
           V KG  VK AGG+GMVL NT  NGEEL+ADSH++P   VGE  G  ++ Y  +    TA+
Sbjct: 431 VQKGQVVKRAGGIGMVLTNTATNGEELVADSHMLPAVAVGEKEGKLIKQYAMTSKKATAS 490

Query: 492 IVFRGTVIGNSPSAPRVAAFSSRGPNYRTAEILKPDVIAPGVNILAGWSGYSSPTSLSID 551
           +   GT IG  PS P VAAFSSRGPN+ + EILKPD++APGVNILA W+G  +P+SLS D
Sbjct: 491 LEILGTRIGIKPS-PVVAAFSSRGPNFLSLEILKPDLLAPGVNILAAWTGDMAPSSLSSD 550

Query: 552 SRRVEFNIISGTSMSCPHVSGVAALLRKAFPTWSPAAIKSALITTSYSLDSSGSPIKDLS 611
            RRV+FNI+SGTSMSCPHVSGVAAL++   P WSPAAIKSAL+TT+Y  D+   P+ D S
Sbjct: 551 PRRVKFNILSGTSMSCPHVSGVAALIKSRHPDWSPAAIKSALMTTAYVHDNMFKPLTDAS 610

Query: 612 TSKESNPFVHGAGHIDPNKALNPGLIYDLTPQDYVSFLCSIGYDSRQIAVFVKDSSYSQL 671
            +  S+P+ HGAGHIDP +A +PGL+YD+ PQ+Y  FLC+      Q+ VF K S  ++ 
Sbjct: 611 GAAPSSPYDHGAGHIDPLRATDPGLVYDIGPQEYFEFLCTQDLSPSQLKVFTKHS--NRT 670

Query: 672 CEHKLS-NPGNLNYPSFSVVFDGEEVVK---YTRTVTNVGDETDVYEVKVESPQGVEISV 731
           C+H L+ NPGNLNYP+ S +F     VK     RTVTNVG     Y+V V   +G  ++V
Sbjct: 671 CKHTLAKNPGNLNYPAISALFPENTHVKAMTLRRTVTNVGPHISSYKVSVSPFKGASVTV 730

Query: 732 LPNKLEFNAKKTTQSYEITFTKISGFKESASFGSIQWSDGSHNVRSPIAVSF 760
            P  L F +K    SY +TF      K    FG + W   +H VRSP+ +++
Sbjct: 731 QPKTLNFTSKHQKLSYTVTFRTRFRMKR-PEFGGLVWKSTTHKVRSPVIITW 776

BLAST of ClCG09G001040 vs. NCBI nr
Match: gi|659124686|ref|XP_008462294.1| (PREDICTED: LOW QUALITY PROTEIN: subtilisin-like protease [Cucumis melo])

HSP 1 Score: 1396.3 bits (3613), Expect = 0.0e+00
Identity = 695/770 (90.26%), Postives = 729/770 (94.68%), Query Frame = 1

Query: 1   MANFHRSSSLFFFFIFCFV-PVIFSRSPDNQETFIVHVSKSEKPSLFSSHRHWHSSILES 60
           MANFH  SS FFFF   FV P++FSRS + QET+IVHVSKSEKPSLFSSH HWHSSILES
Sbjct: 1   MANFHLPSSSFFFFFVLFVFPLVFSRSLETQETYIVHVSKSEKPSLFSSHHHWHSSILES 60

Query: 61  LSPSPHPTKLLYNYEHAVNGFSARITAAQAAELRHVPGVISVIPDRIRQLHTTRTPHFLG 120
           LSPSPHPTKLLYNYE A NGFSARIT AQA ELR VPG+ISVIPDRIRQLHTTRTPHFLG
Sbjct: 61  LSPSPHPTKLLYNYERAANGFSARITTAQAEELRRVPGIISVIPDRIRQLHTTRTPHFLG 120

Query: 121 LADNLGLWPDTNYAEDVIIGVLDTGIWPGRPSFSDEGLSPVPASWKGTCEAGEGFSASTC 180
           LADNLGLW DTNYA+DVIIGVLDTGIWP RPSFSDEGL+PVPA WKGTC+ GEG SA  C
Sbjct: 121 LADNLGLWADTNYADDVIIGVLDTGIWPERPSFSDEGLAPVPARWKGTCDTGEGSSALAC 180

Query: 181 NRKIIGARAYFNGYESNLGRSLR-SSDFKSARDSDGHGTHTASTAAGSFVKNASFFQYAR 240
           NRKIIGARA+F GYESNL  SL+ SSDFKSARD+DGHGTHTASTAAGSFVKNASFFQYAR
Sbjct: 181 NRKIIGARAFFXGYESNLRGSLKGSSDFKSARDTDGHGTHTASTAAGSFVKNASFFQYAR 240

Query: 241 GEARGMASRARIAAYKICWEFGCFDSDILAAMDQAIADGVHIISLSVGSSGRAPAYYRDS 300
           GEARGMASRARIAAYKICWEFGC+DSDILAAMDQAI+DGV +ISLSVGSSGRAPAYYRDS
Sbjct: 241 GEARGMASRARIAAYKICWEFGCYDSDILAAMDQAISDGVDVISLSVGSSGRAPAYYRDS 300

Query: 301 IAIGAFGAMQHGVVVSCSAGNSGPGPYTAVNIAPWILTVGASTIDREFLADVILGDGRVF 360
           IAIGAFGAMQHG+VVSCSAGNSGPGPYTAVNIAPWI+TVGASTIDREFLADVILGD RVF
Sbjct: 301 IAIGAFGAMQHGIVVSCSAGNSGPGPYTAVNIAPWIVTVGASTIDREFLADVILGDNRVF 360

Query: 361 SGVSLYSGEPLGDSKLPLVYGGDCGSRYCYSGSLDPAKVAGKIVVCDRGGNARVAKGGAV 420
           SGVSLYSG+PLGDSKLPLVYGGDCGSRYCYSGSLD +KVAG+IVVCDRGGNARVAKGGAV
Sbjct: 361 SGVSLYSGDPLGDSKLPLVYGGDCGSRYCYSGSLDSSKVAGRIVVCDRGGNARVAKGGAV 420

Query: 421 KSAGGLGMVLANTEENGEELLADSHLIPGTMVGEIAGNKLRNYIHSDPNPTATIVFRGTV 480
           KSAGGLGMVLANTEENGEELLADSHLIPGTMVGEIAGNKLR+YIH+DPNPTATIVFRGTV
Sbjct: 421 KSAGGLGMVLANTEENGEELLADSHLIPGTMVGEIAGNKLRDYIHTDPNPTATIVFRGTV 480

Query: 481 IGNSPSAPRVAAFSSRGPNYRTAEILKPDVIAPGVNILAGWSGYSSPTSLSIDSRRVEFN 540
           IG+SP AP+VA+FSSRGPNYRTAEILKPDVIAPGVNILAGWSGYSSPT L+ID RRVEFN
Sbjct: 481 IGDSPPAPKVASFSSRGPNYRTAEILKPDVIAPGVNILAGWSGYSSPTGLNIDPRRVEFN 540

Query: 541 IISGTSMSCPHVSGVAALLRKAFPTWSPAAIKSALITTSYSLDSSGSPIKDLSTSKESNP 600
           IISGTSMSCPHVSGVAALLRKAFPTWSPAAIKSALITTSYSLDSSGSPIKDL+TS+ESNP
Sbjct: 541 IISGTSMSCPHVSGVAALLRKAFPTWSPAAIKSALITTSYSLDSSGSPIKDLATSEESNP 600

Query: 601 FVHGAGHIDPNKALNPGLIYDLTPQDYVSFLCSIGYDSRQIAVFVKDSSYSQLCEHKLSN 660
           FVHGAGHIDPN+ALNPGLIYDLTPQDYVSFLCSIGYDS+QIAVFVK SSYSQLCEHKLSN
Sbjct: 601 FVHGAGHIDPNQALNPGLIYDLTPQDYVSFLCSIGYDSKQIAVFVKGSSYSQLCEHKLSN 660

Query: 661 PGNLNYPSFSVVFDGEEVVKYTRTVTNVGDETD-VYEVKVESPQGVEISVLPNKLEFNAK 720
           PGNLNYPSFSVVFD  EVVKYTRTVTNVGDET+ VY VKVE+PQGVEISV+PNKLEFN +
Sbjct: 661 PGNLNYPSFSVVFDDGEVVKYTRTVTNVGDETEVVYGVKVEAPQGVEISVVPNKLEFNKE 720

Query: 721 KTTQSYEITFTKISGFKESASFGSIQWSDGSHNVRSPIAVSFNTGSIASM 768
           KTT SYEITFTKI+GFKESASFGSIQWSDG HNVRSPIAVSF TGSIASM
Sbjct: 721 KTTLSYEITFTKINGFKESASFGSIQWSDGIHNVRSPIAVSFKTGSIASM 770

BLAST of ClCG09G001040 vs. NCBI nr
Match: gi|449447904|ref|XP_004141706.1| (PREDICTED: subtilisin-like protease SBT1.7 [Cucumis sativus])

HSP 1 Score: 1393.3 bits (3605), Expect = 0.0e+00
Identity = 697/771 (90.40%), Postives = 728/771 (94.42%), Query Frame = 1

Query: 1   MANFHR-SSSLFFFFIFCFVPVIFSRSPDNQETFIVHVSKSEKPSLFSSHRHWHSSILES 60
           MANFH  SSS FFFF+FCF P+IFSRS +NQET+IVHVSKSEKPSLFSSH HWHSSILES
Sbjct: 1   MANFHLPSSSFFFFFLFCFFPLIFSRSLENQETYIVHVSKSEKPSLFSSHHHWHSSILES 60

Query: 61  LSPSPHPTKLLYNYEHAVNGFSARITAAQAAELRHVPGVISVIPDRIRQLHTTRTPHFLG 120
           LS SPHPTKLLYNYE A NGFSARIT  QA ELR VPG+ISVIPD+IRQLHTTRTPHFLG
Sbjct: 61  LSSSPHPTKLLYNYERAANGFSARITTVQAEELRRVPGIISVIPDQIRQLHTTRTPHFLG 120

Query: 121 LADNLGLWPDTNYAEDVIIGVLDTGIWPGRPSFSDEGLSPVPASWKGTCEAGEGFSASTC 180
           LADNLGLW DTNYA+DVIIGVLDTGIWP RPSFSDEGLSPVPA WKGTC+ GEG SA  C
Sbjct: 121 LADNLGLWADTNYADDVIIGVLDTGIWPERPSFSDEGLSPVPARWKGTCDTGEGVSAFAC 180

Query: 181 NRKIIGARAYFNGYESNLGRSLR-SSDFKSARDSDGHGTHTASTAAGSFVKNASFFQYAR 240
           NRKIIGARAYF GYESNL  SL+ SSDFKSARD++GHGTHTASTAAGSFV NASFFQYAR
Sbjct: 181 NRKIIGARAYFYGYESNLRGSLKVSSDFKSARDTEGHGTHTASTAAGSFVNNASFFQYAR 240

Query: 241 GEARGMASRARIAAYKICWEFGCFDSDILAAMDQAIADGVHIISLSVGSSGRAPAYYRDS 300
           GEARGMASRARIAAYKICWEFGC+DSDILAAMDQAI+DGV +ISLSVGSSGRAPAYYRDS
Sbjct: 241 GEARGMASRARIAAYKICWEFGCYDSDILAAMDQAISDGVDVISLSVGSSGRAPAYYRDS 300

Query: 301 IAIGAFGAMQHGVVVSCSAGNSGPGPYTAVNIAPWILTVGASTIDREFLADVILGDGRVF 360
           IAIGAFGAMQHGVVVSCSAGNSGPGPYTAVNIAPWILTVGASTIDREFLADVILGDGRVF
Sbjct: 301 IAIGAFGAMQHGVVVSCSAGNSGPGPYTAVNIAPWILTVGASTIDREFLADVILGDGRVF 360

Query: 361 SGVSLYSGEPLGDSKLPLVYGGDCGSRYCYSGSLDPAKVAGKIVVCDRGGNARVAKGGAV 420
           SGVSLYSG+PLGDSKL LVYGGDCGSRYCYSGSLD +KVAGKIVVCDRGGNARVAKGGAV
Sbjct: 361 SGVSLYSGDPLGDSKLQLVYGGDCGSRYCYSGSLDSSKVAGKIVVCDRGGNARVAKGGAV 420

Query: 421 KSAGGLGMVLANTEENGEELLADSHLIPGTMVGEIAGNKLRNYIHSDPNPTATIVFRGTV 480
           KSAGGLGMVLANTEENGEELLADSHLIPGTMVG IAGNKLR+YIH+DPNPTATIVFRGTV
Sbjct: 421 KSAGGLGMVLANTEENGEELLADSHLIPGTMVGAIAGNKLRDYIHTDPNPTATIVFRGTV 480

Query: 481 IGNSPSAPRVAAFSSRGPNYRTAEILKPDVIAPGVNILAGWSGYSSPTSLSIDSRRVEFN 540
           IG+SP APRVAAFSSRGPNYRTAEILKPDVIAPGVNILAGWSGYSSPT L+ID RRVEFN
Sbjct: 481 IGDSPPAPRVAAFSSRGPNYRTAEILKPDVIAPGVNILAGWSGYSSPTGLNIDPRRVEFN 540

Query: 541 IISGTSMSCPHVSGVAALLRKAFPTWSPAAIKSALITTSYSLDSSGSPIKDLSTSKESNP 600
           IISGTSMSCPHVSGVAALLRKAFPTWSPAAIKSALITTSYSLDSSG PIKDLSTS+ESNP
Sbjct: 541 IISGTSMSCPHVSGVAALLRKAFPTWSPAAIKSALITTSYSLDSSGKPIKDLSTSEESNP 600

Query: 601 FVHGAGHIDPNKALNPGLIYDLTPQDYVSFLCSIGYDSRQIAVFVKDSSYSQLCEHKLSN 660
           FVHGAGHI+PN+ALNPGLIYDLTPQDYVSFLCSIGYDS+QIAVFVK SSY QLCEHKL+N
Sbjct: 601 FVHGAGHINPNQALNPGLIYDLTPQDYVSFLCSIGYDSKQIAVFVKGSSYFQLCEHKLTN 660

Query: 661 PGNLNYPSFSVVFDGEEVVKYTRTVTNVGDETD-VYEVKVESPQGVEISVLPNKLEFNAK 720
           PGNLNYPSFSVVFD EEVVKYTRTVTNVGDET+ VYEVKVE+PQGV ISV+PNKLEFN +
Sbjct: 661 PGNLNYPSFSVVFDEEEVVKYTRTVTNVGDETEVVYEVKVEAPQGVVISVVPNKLEFNKE 720

Query: 721 KTTQSYEITFTKISGFKESASFGSIQWSDGSHNVRSPIAVSFNT-GSIASM 768
           KTTQSYEITFTKI+GFKESASFGSIQW DG H+VRSPIAVSF T GSIASM
Sbjct: 721 KTTQSYEITFTKINGFKESASFGSIQWGDGIHSVRSPIAVSFKTGGSIASM 771

BLAST of ClCG09G001040 vs. NCBI nr
Match: gi|470140051|ref|XP_004305758.1| (PREDICTED: subtilisin-like protease [Fragaria vesca subsp. vesca])

HSP 1 Score: 1090.1 bits (2818), Expect = 0.0e+00
Identity = 545/769 (70.87%), Postives = 634/769 (82.44%), Query Frame = 1

Query: 7   SSSLFFFFIFCFVPVIFSRSP---DNQETFIVHVSKSEKPSLFSSHRHWHSSILESLSPS 66
           S SLFFF + CFV   FS      D+  TFIVHVSK  KP+LFSS R W++SIL SL PS
Sbjct: 5   SPSLFFFLVLCFVHATFSTDQSDRDSPRTFIVHVSKYSKPALFSSQRRWYTSILRSLPPS 64

Query: 67  PHPTKLLYNYEHAVNGFSARITAAQAAELRHVPGVISVIPDRIRQLHTTRTPHFLGLADN 126
           PHPTKLLY Y  AV+GFSA ++A+QA  L+  P V+SV+PD  RQLHTTRT  FLGLADN
Sbjct: 65  PHPTKLLYTYSRAVHGFSATLSASQAHALQSHPAVLSVVPDMPRQLHTTRTYDFLGLADN 124

Query: 127 LGLWPDTNYAEDVIIGVLDTGIWPGRPSFSDEGLSPVPASWKGTCEAGEGFSASTCNRKI 186
            G+WP+++YA+DVIIGVLDTGIWP RPSFSD GL PVP +WKG C     F AS+CNRKI
Sbjct: 125 FGIWPNSDYADDVIIGVLDTGIWPERPSFSDSGLGPVPKTWKGKCVITGDFPASSCNRKI 184

Query: 187 IGARAYFNGYESNLGRSL-RSSDFKSARDSDGHGTHTASTAAGSFVKNASFFQYARGEAR 246
           IGARAYFNGYES+LG+ +  S++ +S RD++GHGTHTASTA GS V NASF++YA GEAR
Sbjct: 185 IGARAYFNGYESHLGKPMDESNESRSPRDTEGHGTHTASTAGGSRVSNASFYEYASGEAR 244

Query: 247 GMASRARIAAYKICWEFGCFDSDILAAMDQAIADGVHIISLSVGSSGRAPAYYRDSIAIG 306
           GMAS+ARIAAYKICW FGCFDSDILAAMDQAIADGVHIISLSVG+SG AP Y RDSIAIG
Sbjct: 245 GMASKARIAAYKICWTFGCFDSDILAAMDQAIADGVHIISLSVGASGGAPPYDRDSIAIG 304

Query: 307 AFGAMQHGVVVSCSAGNSGPGPYTAVNIAPWILTVGASTIDREFLADVILGDGRVFSGVS 366
           AFGA QHGV+VS SAGNSGPG +TA NIAPWILTVGAST+DREF ADV+LGDGRVF+GVS
Sbjct: 305 AFGAAQHGVLVSASAGNSGPGKFTATNIAPWILTVGASTLDREFPADVVLGDGRVFNGVS 364

Query: 367 LYSGEPLGDSKLPLVYGGDCGSRYCYSGSLDPAKVAGKIVVCDRGGNARVAKGGAVKSAG 426
           LYSGE L D KLPLVYGGDCGSR CYSG+L P+KV GKIVVCDRGGNARVAKG AVK AG
Sbjct: 365 LYSGEGLMDYKLPLVYGGDCGSRLCYSGALQPSKVQGKIVVCDRGGNARVAKGSAVKLAG 424

Query: 427 GLGMVLANTEENGEELLADSHLIPGTMVGEIAGNKLRNYIHSDPNPTATIVFRGTVIGNS 486
           G+GM++ANTEE+GEELLADSHLIP TMVG++A +++R+YI +  N TATI FRGTVIG S
Sbjct: 425 GIGMIMANTEESGEELLADSHLIPATMVGQMAADQIRSYIKTGHNATATIKFRGTVIGTS 484

Query: 487 PSAPRVAAFSSRGPNYRTAEILKPDVIAPGVNILAGWSGYSSPTSLSIDSRRVEFNIISG 546
           P +P+VA+FSSRGPN  T EILKPDVIAPGVNILAGW+G SSPT L ID RRVEFNIISG
Sbjct: 485 PPSPKVASFSSRGPNSLTPEILKPDVIAPGVNILAGWTGASSPTDLDIDPRRVEFNIISG 544

Query: 547 TSMSCPHVSGVAALLRKAFPTWSPAAIKSALITTSYSLDSSGSPIKDLSTSKESNPFVHG 606
           TSMSCPHVSG+AALLRKA+P WSPAAIKSAL+TT+Y+LD+SG+ IKDL+   ES PFVHG
Sbjct: 545 TSMSCPHVSGIAALLRKAYPKWSPAAIKSALVTTAYTLDNSGNKIKDLANGGESTPFVHG 604

Query: 607 AGHIDPNKALNPGLIYDLTPQDYVSFLCSIGYDSRQIAVFVKDSSYSQLC-EHKLSNPGN 666
           AGH+DPN+ALNPGL+YD+   DYV+F+CSIGY  RQIAVF+++ +   +C  + L++PG+
Sbjct: 605 AGHVDPNRALNPGLVYDIDVNDYVAFMCSIGYGPRQIAVFMRELAGDDICARNSLASPGD 664

Query: 667 LNYPSFSVVF-DGEEVVKYTRTVTNVGDETD-VYEVKVESPQGVEISVLPNKLEFNAKKT 726
           LNYPSF+VVF  G E+VKY R VTNVG   D VYEV V++P GVEISV P+KL F+    
Sbjct: 665 LNYPSFAVVFKPGRELVKYKRVVTNVGSVVDAVYEVNVDAPAGVEISVEPSKLVFSEVNQ 724

Query: 727 TQSYEITFTKISGFKESASFGSIQWSDGSHNVRSPIAVSF-NTGSIASM 768
           TQSYE+TF K  G+     +GSI+WSDG H+VRSP+AV + NTG  ASM
Sbjct: 725 TQSYEVTFAKGIGYVNGERYGSIEWSDGRHHVRSPVAVRWSNTGYSASM 773

BLAST of ClCG09G001040 vs. NCBI nr
Match: gi|595966241|ref|XP_007217156.1| (hypothetical protein PRUPE_ppa001739mg [Prunus persica])

HSP 1 Score: 1082.4 bits (2798), Expect = 0.0e+00
Identity = 541/767 (70.53%), Postives = 631/767 (82.27%), Query Frame = 1

Query: 9   SLFFFFIFCFVPVIFSRSPD---NQETFIVHVSKSEKPSLFSSHRHWHSSILESLSPSPH 68
           SLF F +   +P   S  PD     +TFIVHVSKS+KPSLFSSHR W++SI+++L PSPH
Sbjct: 7   SLFLFLLSLLLPATLSIPPDLSDRPKTFIVHVSKSQKPSLFSSHRSWYTSIIQNL-PSPH 66

Query: 69  PTKLLYNYEHAVNGFSARITAAQAAELRHVPGVISVIPDRIRQLHTTRTPHFLGLADNLG 128
           PTKLLY Y+ +V+GFSA +T++QA +L   P V+SV PD+ RQLHTT TP+FLGLAD+ G
Sbjct: 67  PTKLLYTYDRSVHGFSATLTSSQATQLLSHPSVLSVTPDQPRQLHTTHTPNFLGLADSFG 126

Query: 129 LWPDTNYAEDVIIGVLDTGIWPGRPSFSDEGLSPVPASWKGTCEAGEGFSASTCNRKIIG 188
           LWP+++YA+DV+IGVLDTGIWP RPSFSD G+ PVP  WKGTC     F +S CNRKIIG
Sbjct: 127 LWPNSDYADDVVIGVLDTGIWPERPSFSDSGIGPVPTRWKGTCVTTADFPSSACNRKIIG 186

Query: 189 ARAYFNGYESNLGRSL-RSSDFKSARDSDGHGTHTASTAAGSFVKNASFFQYARGEARGM 248
           ARAYFNGYES++GR +  +++ KS RD++GHGTHTASTAAG+ V NASFF YA+G+ARGM
Sbjct: 187 ARAYFNGYESHIGRLMDETTEAKSPRDTEGHGTHTASTAAGAVVANASFFSYAQGDARGM 246

Query: 249 ASRARIAAYKICWEFGCFDSDILAAMDQAIADGVHIISLSVGSSGRAPAYYRDSIAIGAF 308
           A++ARIA YKICW FGCFDSDILAAMDQAIADGV IISLSVG+SG AP Y RDSIAIGAF
Sbjct: 247 ATKARIAVYKICWSFGCFDSDILAAMDQAIADGVDIISLSVGASGNAPPYDRDSIAIGAF 306

Query: 309 GAMQHGVVVSCSAGNSGPGPYTAVNIAPWILTVGASTIDREFLADVILGDGRVFSGVSLY 368
           GA QHGV+VS SAGNSGP P+TA NIAPWILTVGASTIDREF ADV+LGD RV SGVSLY
Sbjct: 307 GAAQHGVLVSASAGNSGPNPFTATNIAPWILTVGASTIDREFPADVVLGDNRVISGVSLY 366

Query: 369 SGEPLGDSKLPLVYGGDCGSRYCYSGSLDPAKVAGKIVVCDRGGNARVAKGGAVKSAGGL 428
           SGEPL D KLPLVYGGDCGSRYCY G+L P+KV GKIVVCDRGGNARVAKGGAVK AGGL
Sbjct: 367 SGEPLVDYKLPLVYGGDCGSRYCYEGALQPSKVQGKIVVCDRGGNARVAKGGAVKLAGGL 426

Query: 429 GMVLANTEENGEELLADSHLIPGTMVGEIAGNKLRNYIHSDPNPTATIVFRGTVIGNSPS 488
           GM+LANTEE+GEELLAD HLIP T VGEIA N++R YI     PTATIVFRGTVIG+SPS
Sbjct: 427 GMILANTEESGEELLADGHLIPATEVGEIAANQIREYIRLSQYPTATIVFRGTVIGSSPS 486

Query: 489 APRVAAFSSRGPNYRTAEILKPDVIAPGVNILAGWSGYSSPTSLSIDSRRVEFNIISGTS 548
           +P+VAAFSSRGPN  T EILKPDVIAPGVNILAGW+G ++PT L ID RRVEFNIISGTS
Sbjct: 487 SPQVAAFSSRGPNSLTPEILKPDVIAPGVNILAGWTGATAPTDLDIDPRRVEFNIISGTS 546

Query: 549 MSCPHVSGVAALLRKAFPTWSPAAIKSALITTSYSLDSSGSPIKDLSTSKESNPFVHGAG 608
           MSCPHVSG+AALLRKAFP WS AAIKSALITT+Y+LD++G  IKDL T +ES PFVHGAG
Sbjct: 547 MSCPHVSGIAALLRKAFPNWSIAAIKSALITTAYTLDNAGKKIKDLGTGEESTPFVHGAG 606

Query: 609 HIDPNKALNPGLIYDLTPQDYVSFLCSIGYDSRQIAVFVKDSSYSQLC-EHKLSNPGNLN 668
           H+DPN+ALNPGLIYDL   DYV+FLCSIGY  RQIAVFV   + S +C  + L++PG+LN
Sbjct: 607 HVDPNRALNPGLIYDLNVNDYVAFLCSIGYSPRQIAVFVGKPTGSDMCTRNSLASPGDLN 666

Query: 669 YPSFSVVFDGEE-VVKYTRTVTNVGDETD-VYEVKVESPQGVEISVLPNKLEFNAKKTTQ 728
           YPSFSVV   ++ ++KY R  TNVG + D VYEV V +P GVEISV P KL F+A+  TQ
Sbjct: 667 YPSFSVVLSSDQGLIKYKRIATNVGGDADAVYEVTVNAPAGVEISVEPRKLVFSAENQTQ 726

Query: 729 SYEITFTKISGFKESASFGSIQWSDGSHNVRSPIAVSFNTG-SIASM 768
           SYE+TF +  G+     +GSI+W+DG H VRSP+AV +++  S+ASM
Sbjct: 727 SYEVTFKRGVGYDGGERYGSIEWTDGRHLVRSPVAVRWSSARSLASM 772

BLAST of ClCG09G001040 vs. NCBI nr
Match: gi|645243879|ref|XP_008228179.1| (PREDICTED: subtilisin-like protease [Prunus mume])

HSP 1 Score: 1077.4 bits (2785), Expect = 0.0e+00
Identity = 538/767 (70.14%), Postives = 629/767 (82.01%), Query Frame = 1

Query: 9   SLFFFFIFCFVPVIFSRSPD---NQETFIVHVSKSEKPSLFSSHRHWHSSILESLSPSPH 68
           SLF F +   +P   S  PD     +TFIVHVSKS+KPSLFSSHR W++SI+++L PSPH
Sbjct: 45  SLFLFLLSLLLPATLSIPPDLSDRPKTFIVHVSKSQKPSLFSSHRSWYTSIIQNL-PSPH 104

Query: 69  PTKLLYNYEHAVNGFSARITAAQAAELRHVPGVISVIPDRIRQLHTTRTPHFLGLADNLG 128
           PTKLLY Y+ +V+GFSA +T++QA +L   P V+SV PD+ RQLHTT TP+FLGLAD+ G
Sbjct: 105 PTKLLYTYDRSVHGFSATLTSSQATQLLSHPSVLSVTPDQPRQLHTTHTPNFLGLADSFG 164

Query: 129 LWPDTNYAEDVIIGVLDTGIWPGRPSFSDEGLSPVPASWKGTCEAGEGFSASTCNRKIIG 188
           LWP+++YA+DV+IGVLDTGIWP RPSFSD G+ P+P  WKGTC     F +S CNRKIIG
Sbjct: 165 LWPNSDYADDVVIGVLDTGIWPERPSFSDSGIGPIPTRWKGTCVTTADFPSSACNRKIIG 224

Query: 189 ARAYFNGYESNLGRSL-RSSDFKSARDSDGHGTHTASTAAGSFVKNASFFQYARGEARGM 248
           ARAYFNGYES++GR +  +++ KS RD++GHGTHTASTAAG+ V NASFF YA+GEARGM
Sbjct: 225 ARAYFNGYESHIGRLMDETAEAKSPRDTEGHGTHTASTAAGAVVANASFFSYAQGEARGM 284

Query: 249 ASRARIAAYKICWEFGCFDSDILAAMDQAIADGVHIISLSVGSSGRAPAYYRDSIAIGAF 308
           A++ARIA YKICW  GCFDSDILAAMDQAIADGV IISLSVG+ GRAP Y RDSIAIGAF
Sbjct: 285 ATKARIAVYKICWSSGCFDSDILAAMDQAIADGVDIISLSVGAGGRAPPYDRDSIAIGAF 344

Query: 309 GAMQHGVVVSCSAGNSGPGPYTAVNIAPWILTVGASTIDREFLADVILGDGRVFSGVSLY 368
           GA QHGV+VS SAGNSGP P+TA NIAPWILTVGASTIDREF ADV+LGD RV SGVSLY
Sbjct: 345 GAAQHGVLVSASAGNSGPNPFTATNIAPWILTVGASTIDREFPADVVLGDNRVISGVSLY 404

Query: 369 SGEPLGDSKLPLVYGGDCGSRYCYSGSLDPAKVAGKIVVCDRGGNARVAKGGAVKSAGGL 428
           SGEPL D KLPLV+GGDCGSRYCY G+L P+KV GKIVVCDRGGNARVAKGGAVK AGGL
Sbjct: 405 SGEPLVDYKLPLVHGGDCGSRYCYEGALQPSKVQGKIVVCDRGGNARVAKGGAVKLAGGL 464

Query: 429 GMVLANTEENGEELLADSHLIPGTMVGEIAGNKLRNYIHSDPNPTATIVFRGTVIGNSPS 488
           GM+LANTEE+GEELLAD HLIP T VGEIA N++R YI     PTATIVFRGTVIG+SPS
Sbjct: 465 GMILANTEESGEELLADGHLIPATEVGEIAANQIREYIRLSQYPTATIVFRGTVIGSSPS 524

Query: 489 APRVAAFSSRGPNYRTAEILKPDVIAPGVNILAGWSGYSSPTSLSIDSRRVEFNIISGTS 548
           +PRVAAFSSRGPN  T EILKPDVIAPGVNILAGW+G ++PT L ID RRVEFNIISGTS
Sbjct: 525 SPRVAAFSSRGPNSLTPEILKPDVIAPGVNILAGWTGATAPTDLDIDPRRVEFNIISGTS 584

Query: 549 MSCPHVSGVAALLRKAFPTWSPAAIKSALITTSYSLDSSGSPIKDLSTSKESNPFVHGAG 608
           MSCPHVSG+AALLRKA+P WS AAIKSALITT+Y+LD++G  I DL T +ES PFVHGAG
Sbjct: 585 MSCPHVSGIAALLRKAYPNWSIAAIKSALITTAYTLDNAGKKINDLGTGEESTPFVHGAG 644

Query: 609 HIDPNKALNPGLIYDLTPQDYVSFLCSIGYDSRQIAVFVKDSSYSQLC-EHKLSNPGNLN 668
           H+DPN+ALNPGL+YDL   DYV+FLCSIGY  RQIAVFV   + S +C  + L++PG+LN
Sbjct: 645 HVDPNRALNPGLVYDLNVNDYVAFLCSIGYSPRQIAVFVGKPTGSDICTRNSLASPGDLN 704

Query: 669 YPSFSVVFDGEE-VVKYTRTVTNVGDETD-VYEVKVESPQGVEISVLPNKLEFNAKKTTQ 728
           YPSFSVV   ++ +VKY R  TNVG + D VYEV V +P GVEISV P KL F+A+  TQ
Sbjct: 705 YPSFSVVLSSDQGLVKYKRIATNVGADADAVYEVTVNAPAGVEISVEPRKLVFSAENQTQ 764

Query: 729 SYEITFTKISGFKESASFGSIQWSDGSHNVRSPIAVSFNTG-SIASM 768
           SYE+TF +  G+     +GSI+W+DG H VRSP+AV +++  S+ASM
Sbjct: 765 SYEVTFKRGVGYDSGERYGSIEWTDGRHLVRSPVAVRWSSARSLASM 810

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
SBT14_ARATH5.7e-28763.94Subtilisin-like protease SBT1.4 OS=Arabidopsis thaliana GN=SBT1.4 PE=2 SV=1[more]
SBT17_ARATH6.6e-23556.05Subtilisin-like protease SBT1.7 OS=Arabidopsis thaliana GN=SBT1.7 PE=1 SV=1[more]
SBT18_ARATH4.6e-22053.60Subtilisin-like protease SBT1.8 OS=Arabidopsis thaliana GN=SBT1.8 PE=2 SV=1[more]
SBT15_ARATH5.6e-21851.10Subtilisin-like protease SBT1.5 OS=Arabidopsis thaliana GN=SBT1.5 PE=2 SV=1[more]
SBT13_ARATH4.0e-20848.96Subtilisin-like protease SBT1.3 OS=Arabidopsis thaliana GN=SBT1.3 PE=2 SV=1[more]
Match NameE-valueIdentityDescription
A0A0A0K6Z1_CUCSA0.0e+0090.40Subtilase family protein OS=Cucumis sativus GN=Csa_7G448910 PE=4 SV=1[more]
M5XH18_PRUPE0.0e+0070.53Uncharacterized protein OS=Prunus persica GN=PRUPE_ppa001739mg PE=4 SV=1[more]
A0A061GCZ9_THECC0.0e+0070.79Subtilase family protein OS=Theobroma cacao GN=TCM_029155 PE=4 SV=1[more]
M1A1D2_SOLTU0.0e+0068.77Uncharacterized protein OS=Solanum tuberosum GN=PGSC0003DMG400004891 PE=4 SV=1[more]
A0A059AIP6_EUCGR1.7e-31068.09Uncharacterized protein OS=Eucalyptus grandis GN=EUGRSUZ_J02805 PE=4 SV=1[more]
Match NameE-valueIdentityDescription
AT3G14067.13.2e-28863.94 Subtilase family protein[more]
AT5G67360.13.7e-23656.05 Subtilase family protein[more]
AT2G05920.12.6e-22153.60 Subtilase family protein[more]
AT3G14240.13.2e-21951.10 Subtilase family protein[more]
AT5G51750.12.3e-20948.96 subtilase 1.3[more]
Match NameE-valueIdentityDescription
gi|659124686|ref|XP_008462294.1|0.0e+0090.26PREDICTED: LOW QUALITY PROTEIN: subtilisin-like protease [Cucumis melo][more]
gi|449447904|ref|XP_004141706.1|0.0e+0090.40PREDICTED: subtilisin-like protease SBT1.7 [Cucumis sativus][more]
gi|470140051|ref|XP_004305758.1|0.0e+0070.87PREDICTED: subtilisin-like protease [Fragaria vesca subsp. vesca][more]
gi|595966241|ref|XP_007217156.1|0.0e+0070.53hypothetical protein PRUPE_ppa001739mg [Prunus persica][more]
gi|645243879|ref|XP_008228179.1|0.0e+0070.14PREDICTED: subtilisin-like protease [Prunus mume][more]
The following terms have been associated with this gene:
Vocabulary: INTERPRO
TermDefinition
IPR000209Peptidase_S8/S53_dom
IPR003137PA_domain
IPR010259S8pro/Inhibitor_I9
IPR015500Peptidase_S8_subtilisin-rel
IPR023828Peptidase_S8_Ser-AS
Vocabulary: Molecular Function
TermDefinition
GO:0004252serine-type endopeptidase activity
Vocabulary: Biological Process
TermDefinition
GO:0006508proteolysis
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0006508 proteolysis
biological_process GO:0009086 methionine biosynthetic process
biological_process GO:0000394 RNA splicing, via endonucleolytic cleavage and ligation
biological_process GO:0010223 secondary shoot formation
biological_process GO:0009664 plant-type cell wall organization
biological_process GO:0009832 plant-type cell wall biogenesis
biological_process GO:0010075 regulation of meristem growth
biological_process GO:0048653 anther development
cellular_component GO:0005774 vacuolar membrane
cellular_component GO:0009505 plant-type cell wall
cellular_component GO:0048046 apoplast
cellular_component GO:0005794 Golgi apparatus
cellular_component GO:0009506 plasmodesma
cellular_component GO:0005575 cellular_component
cellular_component GO:0016021 integral component of membrane
cellular_component GO:0016020 membrane
molecular_function GO:0004252 serine-type endopeptidase activity
molecular_function GO:0016787 hydrolase activity
molecular_function GO:0008233 peptidase activity
molecular_function GO:0008236 serine-type peptidase activity

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
ClCG09G001040.1ClCG09G001040.1mRNA


Analysis Name: InterPro Annotations of watermelon (Charleston Gray)
Date Performed: 2016-09-28
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR000209Peptidase S8/S53 domainGENE3DG3DSA:3.40.50.200coord: 482..616
score: 2.2E-79coord: 99..342
score: 2.2
IPR000209Peptidase S8/S53 domainPFAMPF00082Peptidase_S8coord: 134..591
score: 2.4
IPR000209Peptidase S8/S53 domainunknownSSF52743Subtilisin-likecoord: 488..616
score: 2.88E-78coord: 109..381
score: 2.88
IPR003137PA domainPFAMPF02225PAcoord: 375..458
score: 5.
IPR010259Peptidase S8 propeptide/proteinase inhibitor I9GENE3DG3DSA:3.30.70.80coord: 32..98
score: 5.
IPR010259Peptidase S8 propeptide/proteinase inhibitor I9PFAMPF05922Inhibitor_I9coord: 32..110
score: 1.3
IPR015500Peptidase S8, subtilisin-relatedPRINTSPR00723SUBTILISINcoord: 541..557
score: 2.0E-15coord: 133..152
score: 2.0E-15coord: 211..224
score: 2.0
IPR015500Peptidase S8, subtilisin-relatedPANTHERPTHR10795PROPROTEIN CONVERTASE SUBTILISIN/KEXINcoord: 1..767
score:
IPR023828Peptidase S8, subtilisin, Ser-active sitePROSITEPS00138SUBTILASE_SERcoord: 542..552
scor
NoneNo IPR availableGENE3DG3DSA:3.50.30.30coord: 358..467
score: 2.
NoneNo IPR availablePANTHERPTHR10795:SF331SUBFAMILY NOT NAMEDcoord: 1..767
score:
NoneNo IPR availableunknownSSF52025PA domaincoord: 363..465
score: 7.5

The following gene(s) are orthologous to this gene:
GeneOrthologueOrganismBlock
ClCG09G001040Csa1G597040Cucumber (Chinese Long) v2cuwcgB000
ClCG09G001040Csa7G448910Cucumber (Chinese Long) v2cuwcgB524
ClCG09G001040MELO3C015526Melon (DHL92) v3.5.1mewcgB227
ClCG09G001040MELO3C024346Melon (DHL92) v3.5.1mewcgB174
ClCG09G001040Cla011155Watermelon (97103) v1wcgwmB021
ClCG09G001040Cla015540Watermelon (97103) v1wcgwmB007
ClCG09G001040Cla97C03G065250Watermelon (97103) v2wcgwmbB016
ClCG09G001040Cla97C09G163090Watermelon (97103) v2wcgwmbB035
ClCG09G001040Bhi02G001504Wax gourdwcgwgoB077
ClCG09G001040Bhi09G000258Wax gourdwcgwgoB068
ClCG09G001040CSPI01G28750Wild cucumber (PI 183967)cpiwcgB000
ClCG09G001040CSPI07G22450Wild cucumber (PI 183967)cpiwcgB553
ClCG09G001040Cucsa.360170Cucumber (Gy14) v1cgywcgB610
ClCG09G001040Cucsa.141800Cucumber (Gy14) v1cgywcgB240
ClCG09G001040CmaCh10G007690Cucurbita maxima (Rimu)cmawcgB041
ClCG09G001040CmaCh11G007350Cucurbita maxima (Rimu)cmawcgB079
ClCG09G001040CmaCh08G008040Cucurbita maxima (Rimu)cmawcgB778
ClCG09G001040CmaCh17G006810Cucurbita maxima (Rimu)cmawcgB308
ClCG09G001040CmoCh08G007750Cucurbita moschata (Rifu)cmowcgB779
ClCG09G001040CmoCh17G006600Cucurbita moschata (Rifu)cmowcgB301
ClCG09G001040CmoCh11G007480Cucurbita moschata (Rifu)cmowcgB069
ClCG09G001040CmoCh10G008000Cucurbita moschata (Rifu)cmowcgB034
ClCG09G001040Lsi02G028920Bottle gourd (USVL1VR-Ls)lsiwcgB160
ClCG09G001040Cp4.1LG18g03160Cucurbita pepo (Zucchini)cpewcgB314
ClCG09G001040Cp4.1LG04g11050Cucurbita pepo (Zucchini)cpewcgB581
ClCG09G001040Cp4.1LG12g09230Cucurbita pepo (Zucchini)cpewcgB128
ClCG09G001040Cp4.1LG17g04100Cucurbita pepo (Zucchini)cpewcgB284
ClCG09G001040CsGy7G020730Cucumber (Gy14) v2cgybwcgB484
ClCG09G001040MELO3C015526.2Melon (DHL92) v3.6.1medwcgB217
ClCG09G001040MELO3C024346.2Melon (DHL92) v3.6.1medwcgB168
ClCG09G001040Carg12953Silver-seed gourdcarwcgB0092
ClCG09G001040CsaV3_7G034370Cucumber (Chinese Long) v3cucwcgB546
The following gene(s) are paralogous to this gene:
GeneParalogueOrganismBlock
ClCG09G001040ClCG03G015030Watermelon (Charleston Gray)wcgwcgB018