Lsi02G015280 (gene) Bottle gourd (USVL1VR-Ls)

Overview
NameLsi02G015280
Typegene
OrganismLagenaria siceraria (Bottle gourd (USVL1VR-Ls))
DescriptionMatrix metalloproteinase
Locationchr02 : 21170239 .. 21171285 (+)
Genome Viewer
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: CDS
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGGCATTAGAAGAATTATCATTCTTGACTTTCACTCTTCTCCTTCTTACCTTCTTCCCTTTAATATCATCTTCTCAGCCAAACTCTGCACATTCCCATGGAAAATCACCATCACAATTTGTATTTCTGAACCAACTTCATGGATCCCAAAAGGGTGACAAAGTTGAAGGTATCCACCAGCTGAAGAAATACCTCCACCAATTCGGCTACTTGAACGATGCTCAAATTCATTCTGAAAATAATGACGACGAGTTCGACGAGTTGTTAGAATCCGCCATTAAAACGTACCAAAAGAATTACAATCTCAAAGTCACAGGGAGTCTTGATGCTATGACAATAGCTCAAATGTCGAAGCCTCGATGCGGAGTTGCGGATATCATCCATGGTAATACTTGGATGAGATCAAGCAAAAAAAGAAAACAACTTGAACATGGAATTGGCCATTTTCATAGGGTTTCTCACTTTGCTTTCTTTGAAGGAAATCCCAAGTGGCCTACTACTAAATCCCACCTCACTTATGGTAAAACTCAAAAATTTAAATGGATGGATGGTAGTAAATTTAATTATATCAATGCTTCAAAAAAATATAATCTTTTATTCATATTCTCAACAGGGTTCGTTGCCGGAACTCCACCGGAAACGGCGAGTCCGGTTGCTCGAGCTTTTGCTACTTGGGCAGCGAACTCTCACTTCACCTTCTCCCAAGCTTTGGACAACCAAACTTCAGACATCAAAATAGGGTTTGAAAGTGGTGATCATGGAGATGGATATCCATTCGATGGTGTTGGAGGAGTGATAGCCCATGCTTTTTCGCCACCGGATGGCCGGTTTCATCTCGATGCCGAGGAGTCTTGGGCGGCCGGTGTCATCTCCGGTTCGTTCGATTTGGAGACGGTGGCATTGCATGAGATTGGACACCTTCTAGGCCTTCAACATAGCTCCATTGAAAGGGCTATCATGTACCCATCAGTTCCTGAAGGAGCTTCCAAGGGTTTGCATGCCGATGATATTGAAGGCATTAAGGCTTTATATAATAACACTCTTTAA

mRNA sequence

ATGGCATTAGAAGAATTATCATTCTTGACTTTCACTCTTCTCCTTCTTACCTTCTTCCCTTTAATATCATCTTCTCAGCCAAACTCTGCACATTCCCATGGAAAATCACCATCACAATTTGTATTTCTGAACCAACTTCATGGATCCCAAAAGGGTGACAAAGTTGAAGGTATCCACCAGCTGAAGAAATACCTCCACCAATTCGGCTACTTGAACGATGCTCAAATTCATTCTGAAAATAATGACGACGAGTTCGACGAGTTGTTAGAATCCGCCATTAAAACGTACCAAAAGAATTACAATCTCAAAGTCACAGGGAGTCTTGATGCTATGACAATAGCTCAAATGTCGAAGCCTCGATGCGGAGTTGCGGATATCATCCATGCGAACTCTCACTTCACCTTCTCCCAAGCTTTGGACAACCAAACTTCAGACATCAAAATAGGGTTTGAAAGTGGTGATCATGGAGATGGATATCCATTCGATGGTGTTGGAGGAGTGATAGCCCATGCTTTTTCGCCACCGGATGGCCGGTTTCATCTCGATGCCGAGGAGTCTTGGGCGGCCGGTGTCATCTCCGGTTCGTTCGATTTGGAGACGGTGGCATTGCATGAGATTGGACACCTTCTAGGCCTTCAACATAGCTCCATTGAAAGGGCTATCATGTACCCATCAGTTCCTGAAGGAGCTTCCAAGGGTTTGCATGCCGATGATATTGAAGGCATTAAGGCTTTATATAATAACACTCTTTAA

Coding sequence (CDS)

ATGGCATTAGAAGAATTATCATTCTTGACTTTCACTCTTCTCCTTCTTACCTTCTTCCCTTTAATATCATCTTCTCAGCCAAACTCTGCACATTCCCATGGAAAATCACCATCACAATTTGTATTTCTGAACCAACTTCATGGATCCCAAAAGGGTGACAAAGTTGAAGGTATCCACCAGCTGAAGAAATACCTCCACCAATTCGGCTACTTGAACGATGCTCAAATTCATTCTGAAAATAATGACGACGAGTTCGACGAGTTGTTAGAATCCGCCATTAAAACGTACCAAAAGAATTACAATCTCAAAGTCACAGGGAGTCTTGATGCTATGACAATAGCTCAAATGTCGAAGCCTCGATGCGGAGTTGCGGATATCATCCATGCGAACTCTCACTTCACCTTCTCCCAAGCTTTGGACAACCAAACTTCAGACATCAAAATAGGGTTTGAAAGTGGTGATCATGGAGATGGATATCCATTCGATGGTGTTGGAGGAGTGATAGCCCATGCTTTTTCGCCACCGGATGGCCGGTTTCATCTCGATGCCGAGGAGTCTTGGGCGGCCGGTGTCATCTCCGGTTCGTTCGATTTGGAGACGGTGGCATTGCATGAGATTGGACACCTTCTAGGCCTTCAACATAGCTCCATTGAAAGGGCTATCATGTACCCATCAGTTCCTGAAGGAGCTTCCAAGGGTTTGCATGCCGATGATATTGAAGGCATTAAGGCTTTATATAATAACACTCTTTAA

Protein sequence

MALEELSFLTFTLLLLTFFPLISSSQPNSAHSHGKSPSQFVFLNQLHGSQKGDKVEGIHQLKKYLHQFGYLNDAQIHSENNDDEFDELLESAIKTYQKNYNLKVTGSLDAMTIAQMSKPRCGVADIIHANSHFTFSQALDNQTSDIKIGFESGDHGDGYPFDGVGGVIAHAFSPPDGRFHLDAEESWAAGVISGSFDLETVALHEIGHLLGLQHSSIERAIMYPSVPEGASKGLHADDIEGIKALYNNTL
Homology
[Close]
BLAST of Lsi02G015280 vs. Swiss-Prot
Match: 2MMP_ARATH (Metalloendoproteinase 2-MMP OS=Arabidopsis thaliana GN=2MMP PE=1 SV=1)

HSP 1 Score: 138.3 bits (347), Expect = 1.2e-31
Identity = 69/123 (56.10%), Postives = 88/123 (71.54%), Query Frame = 1 

Query: 132 HFTFSQALDNQTSDIKIGFESGDHGDGYPFDGVGGVIAHAFSPPDGRFHLDAEESWAAG- 191
           +FT S++    TSDI IGF +GDHGDG PFDGV G +AHAFSPP G+FHLDA+E+W    
Sbjct: 203 NFTLSESFS--TSDITIGFYTGDHGDGEPFDGVLGTLAHAFSPPSGKFHLDADENWVVSG 262

Query: 192 ------VISGSFDLETVALHEIGHLLGLQHSSIERAIMYPSVPEGASK-GLHADDIEGIK 247
                  ++ + DLE+VA+HEIGHLLGL HSS+E +IMYP++  G  K  L  DD+EGI+
Sbjct: 263 DLDSFLSVTAAVDLESVAVHEIGHLLGLGHSSVEESIMYPTITTGKRKVDLTNDDVEGIQ 322

[Close]
BLAST of Lsi02G015280 vs. Swiss-Prot
Match: MEP1_SOYBN (Metalloendoproteinase 1 OS=Glycine max PE=1 SV=2)

HSP 1 Score: 130.6 bits (327), Expect = 2.4e-29
Identity = 65/122 (53.28%), Postives = 83/122 (68.03%), Query Frame = 1 

Query: 132 HFTFSQALDNQTSDIKIGFESGDHGDGYPFDGVGGVIAHAFSPPDGRFHLDAEESWAA-- 191
           +  F +    +T++IKI F S +HGD YPFDG GG++ HAF+P DGR H DA+E W A  
Sbjct: 180 NIAFQETTSYETANIKILFASKNHGDPYPFDGPGGILGHAFAPTDGRCHFDADEYWVASG 239

Query: 192 ----GVISGSFDLETVALHEIGHLLGLQHSSIERAIMYPSVPEGASK-GLHADDIEGIKA 247
                 ++ +FDLE+VA+HEIGHLLGL HSS  RAIMYPS+P    K  L  DDI+GI+ 
Sbjct: 240 DVTKSPVTSAFDLESVAVHEIGHLLGLGHSSDLRAIMYPSIPPRTRKVNLAQDDIDGIRK 299

[Close]
BLAST of Lsi02G015280 vs. Swiss-Prot
Match: 3MMP_ARATH (Metalloendoproteinase 3-MMP OS=Arabidopsis thaliana GN=3MMP PE=1 SV=1)

HSP 1 Score: 130.2 bits (326), Expect = 3.2e-29
Identity = 66/122 (54.10%), Postives = 82/122 (67.21%), Query Frame = 1 

Query: 134 TFSQALDNQTSDIKIGFESGDHGDGYPFDGVGGVIAHAFSPPDGRFHLDAEESWAAG--- 193
           TF++     TSDI IGF SG+HGDG PFDG    +AHAFSPP G FHLD EE+W      
Sbjct: 207 TFTRVERFSTSDISIGFYSGEHGDGEPFDGPMRTLAHAFSPPTGHFHLDGEENWIVSGEG 266

Query: 194 -----VISGSFDLETVALHEIGHLLGLQHSSIERAIMYPSVPEGASK-GLHADDIEGIKA 247
                 +S + DLE+VA+HEIGHLLGL HSS+E +IMYP++  G  K  L  DD+EG++ 
Sbjct: 267 GDGFISVSEAVDLESVAVHEIGHLLGLGHSSVEGSIMYPTIRTGRRKVDLTTDDVEGVQY 326

[Close]
BLAST of Lsi02G015280 vs. Swiss-Prot
Match: 1MMP_ARATH (Metalloendoproteinase 1-MMP OS=Arabidopsis thaliana GN=1MMP PE=1 SV=1)

HSP 1 Score: 125.6 bits (314), Expect = 7.9e-28
Identity = 64/119 (53.78%), Postives = 79/119 (66.39%), Query Frame = 1 

Query: 134 TFSQALDNQTSDIKIGFESGDHGDGYPFDGVGGVIAHAFSPPDGRFHLDAEESWAA---- 193
           +F +  D  T+D+KIGF +GDHGDG PFDGV G +AHAF+P +GR HLDA E+W      
Sbjct: 200 SFEEVDDFTTADLKIGFYAGDHGDGLPFDGVLGTLAHAFAPENGRLHLDAAETWIVDDDL 259

Query: 194 -GVISGSFDLETVALHEIGHLLGLQHSSIERAIMYPSV-PEGASKGLHADDIEGIKALY 247
            G    + DLE+VA HEIGHLLGL HSS E A+MYPS+ P      L  DD+ G+  LY
Sbjct: 260 KGSSEVAVDLESVATHEIGHLLGLGHSSQESAVMYPSLRPRTKKVDLTVDDVAGVLKLY 318

[Close]
BLAST of Lsi02G015280 vs. Swiss-Prot
Match: 4MMP_ARATH (Metalloendoproteinase 4-MMP OS=Arabidopsis thaliana GN=4MMP PE=1 SV=1)

HSP 1 Score: 124.4 bits (311), Expect = 1.7e-27
Identity = 62/118 (52.54%), Postives = 82/118 (69.49%), Query Frame = 1 

Query: 134 TFSQALDNQTSDIKIGFESGDHGDGYPFDGVGGVIAHAFSPPDGRFHLDAEESWAAGV-- 193
           +F +  D   +DIKIGF +GDHGDG PFDGV GV+AH FSP +GR HLD  E+WA     
Sbjct: 178 SFIETEDYVIADIKIGFFNGDHGDGEPFDGVLGVLAHTFSPENGRLHLDKAETWAVDFDE 237

Query: 194 --ISGSFDLETVALHEIGHLLGLQHSSIERAIMYPSV-PEGASKGLHADDIEGIKALY 247
              S + DLE+VA+HEIGH+LGL HSS++ A MYP++ P      L+ DD+ G+++LY
Sbjct: 238 EKSSVAVDLESVAVHEIGHVLGLGHSSVKDAAMYPTLKPRSKKVNLNMDDVVGVQSLY 295

[Close]
BLAST of Lsi02G015280 vs. TrEMBL
Match: A0A0A0KS39_CUCSA (Uncharacterized protein OS=Cucumis sativus GN=Csa_5G632120 PE=3 SV=1)

HSP 1 Score: 236.5 bits (602), Expect = 3.5e-59
Identity = 125/156 (80.13%), Postives = 132/156 (84.62%), Query Frame = 1 

Query: 1   MALEELSFLTFTLLLLTFFPLISSSQPNSAHSHGKSPSQFVFLNQLHGSQKGDKVEGIHQ 60
           MALEELSF TFTLLLLTFFPLISSSQ N +HSHGK+ SQFVFLNQL+GSQKGDKVEGIHQ
Sbjct: 1   MALEELSFFTFTLLLLTFFPLISSSQSNHSHSHGKASSQFVFLNQLYGSQKGDKVEGIHQ 60

Query: 61  LKKYLHQFGYLN-DAQIHSENNDDEFDELLESAIKTYQKNYNLKVTGSLDAMTIAQMSKP 120
           LKKYLHQFGYLN DAQIHSENNDDEFDELLE+AIKTYQKNYNLKVTG+LDAMTIAQMSKP
Sbjct: 61  LKKYLHQFGYLNEDAQIHSENNDDEFDELLETAIKTYQKNYNLKVTGTLDAMTIAQMSKP 120

Query: 121 RCGVADIIHANSHFTFSQALDNQTSDIKIGFESGDH 156
           RCGVADIIH N+    S+        I   F S  H
Sbjct: 121 RCGVADIIHGNTWMRSSKKRKQLEHGIVGHFHSVSH 156

[Close]
BLAST of Lsi02G015280 vs. TrEMBL
Match: M5WU56_PRUPE (Uncharacterized protein (Fragment) OS=Prunus persica GN=PRUPE_ppa014830mg PE=3 SV=1)

HSP 1 Score: 235.0 bits (598), Expect = 1.0e-58
Identity = 134/293 (45.73%), Postives = 172/293 (58.70%), Query Frame = 1 

Query: 2   ALEELSFLTFTLLLLTFFPLISSSQPNSAHSHGKSPSQFVFLNQLHGSQKGDKVEGIHQL 61
           ++  +  L+F+     F PLI  +   +      S S F FL  L G  KGDKV+GI  L
Sbjct: 5   SINGIKILSFSPPHPPFSPLIPCNFIRNPQQQNTS-SPFEFLEHLKGCHKGDKVQGIQDL 64

Query: 62  KKYLHQFGYLNDAQIHSENNDDEFDELLESAIKTYQKNYNLKVTGSLDAMTIAQMSKPRC 121
           KKYL +FGYL+    +   NDD+FD+ LESAIKTYQ NY+LK TG+LDA T++ M  PRC
Sbjct: 65  KKYLGKFGYLSSNN-NGHFNDDDFDDQLESAIKTYQINYHLKATGALDAKTVSNMMMPRC 124

Query: 122 GVADI---------------------IHANSHFTF------------------------- 181
           GVADI                      H  +H+TF                         
Sbjct: 125 GVADIINGTSSMRSGKQRHPHHHHHGGHTVAHYTFFRGNPKWPASKYHLTYAFLQDTPAE 184

Query: 182 SQALDNQTSDIKIGFESGDHGDGYPFDGVGGVIAHAFSPPDGRFHLDAEESWAAGVISGS 241
           +    NQ  D+ + F  G+HGDG PFDG GG IAHAF+P +GRFH DA+E ++ G +SG+
Sbjct: 185 ATGPSNQNPDLTVSFHRGNHGDGAPFDGPGGTIAHAFAPTNGRFHYDADERFSVGAVSGA 244

Query: 242 FDLETVALHEIGHLLGLQHSSIERAIMYPSVPEGAS-KGLHADDIEGIKALYN 248
           +DLETVALHEIGHLLGL HSS++ AIMYP++  G + + LH DDI+GIKALYN
Sbjct: 245 YDLETVALHEIGHLLGLGHSSVQGAIMYPTISPGVTQQSLHGDDIQGIKALYN 295

[Close]
BLAST of Lsi02G015280 vs. TrEMBL
Match: A0A059A5V0_EUCGR (Uncharacterized protein (Fragment) OS=Eucalyptus grandis GN=EUGRSUZ_K02793 PE=3 SV=1)

HSP 1 Score: 213.8 bits (543), Expect = 2.4e-52
Identity = 120/236 (50.85%), Postives = 146/236 (61.86%), Query Frame = 1 

Query: 46  LHGSQKGDKVEGIHQLKKYLHQFGYLNDAQ-----IHSENNDDEFDELLESAIKTYQKNY 105
           L G  KGD   GI +LK+YL +FGYLN  Q     IH++  DD+FD+LLE A+KTYQ NY
Sbjct: 2   LQGCHKGDHTGGIRELKRYLEKFGYLNYNQSGHPTIHAD--DDDFDDLLEFAVKTYQLNY 61

Query: 106 NLKVTGSLDAMTIAQMSKPRCGVADIIHAN-------------------------SHFTF 165
           +L VTG+LD  T ++M +P    A   H                           + FTF
Sbjct: 62  HLNVTGTLDPQTTSKMMRPPRWPASNYHLTYGFLPGTPEAATAPVARAFAKWTSVTRFTF 121

Query: 166 SQALDNQTSDIKIGFESGDHGDGY--PFDGVGGVIAHAFSPPDGRFHLDAEESWAAGVIS 225
           S   D+Q ++I+IGF  GDHGDG    FDG GG +AHAF P  G FH DA+E WA    S
Sbjct: 122 SYVQDHQFANIQIGFARGDHGDGAHNSFDGPGGTLAHAFPPTSGLFHYDADEKWAVDPPS 181

Query: 226 GS--FDLETVALHEIGHLLGLQHSSIERAIMYPSVPEGASKGLHADDIEGIKALYN 248
           GS  F++ETVALHEIGHLLGL HS +E AIMY S+  G +KGLHADDI+GIKALYN
Sbjct: 182 GSAAFNVETVALHEIGHLLGLGHSDVEAAIMYSSIAPGTTKGLHADDIQGIKALYN 235

[Close]
BLAST of Lsi02G015280 vs. TrEMBL
Match: S1S448_THECC (Matrix metalloproteinase, putative OS=Theobroma cacao GN=TCM_046070 PE=3 SV=1)

HSP 1 Score: 200.7 bits (509), Expect = 2.1e-48
Identity = 104/204 (50.98%), Postives = 137/204 (67.16%), Query Frame = 1 

Query: 55  VEGIHQLKKYLHQFGYLNDAQIHSENNDDEFDELLESAIKTYQKNYNLKVTGSLDAMTIA 114
           V+ IH+LKKYL QF YL+ ++  +  NDD+FD+LLESAIKTYQ N++L    +LD  T++
Sbjct: 35  VKDIHKLKKYLEQFDYLSYSKNKTHANDDDFDDLLESAIKTYQLNFHLNSNRALDTETVS 94

Query: 115 QMSKPRCGVADIIH------------------ANSHFTFSQALDNQTSDIKIGFESGDHG 174
           +M  PR GVADII+                  AN++F FS+  + + ++I IGF+  DHG
Sbjct: 95  KMMMPRYGVADIINGTRVNVVSPVAGAFQTWAANTYFRFSRIYNYRDTNITIGFQRRDHG 154

Query: 175 DGYPFDGVGGVIAHAFSPPDGRFHLDAEESWAAGVISGSFDLETVALHEIGHLLGLQHSS 234
           DG PF+  GG IAHAF+P  GRF+ DA+E+W+     G+  LET+ALHEI HLLGL H+S
Sbjct: 155 DGNPFEDPGGTIAHAFAPTIGRFNYDADETWSVSARPGNMHLETIALHEIRHLLGLSHNS 214

Query: 235 IERAIMYPSVPEGASKGLHADDIE 241
           IE AIMYPS+ E  SK L  DDIE
Sbjct: 215 IENAIMYPSIAERTSKRLDRDDIE 238

[Close]
BLAST of Lsi02G015280 vs. TrEMBL
Match: A0A151R3R2_CAJCA (Metalloendoproteinase 1 OS=Cajanus cajan GN=KK1_041636 PE=3 SV=1)

HSP 1 Score: 197.6 bits (501), Expect = 1.8e-47
Identity = 117/263 (44.49%), Postives = 151/263 (57.41%), Query Frame = 1 

Query: 19  FPLISSSQPNSAHSHGKSPSQFVFLNQLHGSQKGDKVEGIHQLKKYLHQFGYLNDAQIHS 78
           FP +SS  P+ A   G   +         G  +GD  +G+  LK Y  +FGY+  A    
Sbjct: 30  FPNVSSWLPDYAPLGGAWDA----FRNFTGCHRGDNYDGLANLKSYFQRFGYIPHAP--P 89

Query: 79  ENNDDEFDELLESAIKTYQKNYNLKVTGSLDAMTIAQMSKPRCGVADIIHANSHFTFSQA 138
            N  D+FD+ LESAIKTYQKN+NL VTG LD  T+ Q+  PRCGVADII+  +  T +  
Sbjct: 90  SNFSDDFDDALESAIKTYQKNFNLNVTGVLDDATLRQIVLPRCGVADIINGTT--TMNAG 149

Query: 139 LDNQTS--------------------------DIKIGFESGDHGDGYPFDGVGGVIAHAF 198
            +NQT+                          DI+IGF SGDHGDG PFDG  G +AHAF
Sbjct: 150 RENQTAVFAAAFARWAEVTSLRFRETASYFGADIRIGFFSGDHGDGEPFDGSLGTLAHAF 209

Query: 199 SPPDGRFHLDAEESWAA------GVISGSFDLETVALHEIGHLLGLQHSSIERAIMYPSV 249
           SP +GRFHLDA E W          ++ + DLE+VA+HEIGHLLGL HSS+E A+M+P++
Sbjct: 210 SPTNGRFHLDAAEDWVVSGDVTRSALATAVDLESVAVHEIGHLLGLGHSSVEEAVMFPTI 269

[Close]
BLAST of Lsi02G015280 vs. TAIR10
Match: AT1G70170.1 (AT1G70170.1 matrix metalloproteinase)

HSP 1 Score: 138.3 bits (347), Expect = 6.6e-33
Identity = 69/123 (56.10%), Postives = 88/123 (71.54%), Query Frame = 1 

Query: 132 HFTFSQALDNQTSDIKIGFESGDHGDGYPFDGVGGVIAHAFSPPDGRFHLDAEESWAAG- 191
           +FT S++    TSDI IGF +GDHGDG PFDGV G +AHAFSPP G+FHLDA+E+W    
Sbjct: 203 NFTLSESFS--TSDITIGFYTGDHGDGEPFDGVLGTLAHAFSPPSGKFHLDADENWVVSG 262

Query: 192 ------VISGSFDLETVALHEIGHLLGLQHSSIERAIMYPSVPEGASK-GLHADDIEGIK 247
                  ++ + DLE+VA+HEIGHLLGL HSS+E +IMYP++  G  K  L  DD+EGI+
Sbjct: 263 DLDSFLSVTAAVDLESVAVHEIGHLLGLGHSSVEESIMYPTITTGKRKVDLTNDDVEGIQ 322

[Close]
BLAST of Lsi02G015280 vs. TAIR10
Match: AT1G24140.1 (AT1G24140.1 Matrixin family protein)

HSP 1 Score: 130.2 bits (326), Expect = 1.8e-30
Identity = 66/122 (54.10%), Postives = 82/122 (67.21%), Query Frame = 1 

Query: 134 TFSQALDNQTSDIKIGFESGDHGDGYPFDGVGGVIAHAFSPPDGRFHLDAEESWAAG--- 193
           TF++     TSDI IGF SG+HGDG PFDG    +AHAFSPP G FHLD EE+W      
Sbjct: 207 TFTRVERFSTSDISIGFYSGEHGDGEPFDGPMRTLAHAFSPPTGHFHLDGEENWIVSGEG 266

Query: 194 -----VISGSFDLETVALHEIGHLLGLQHSSIERAIMYPSVPEGASK-GLHADDIEGIKA 247
                 +S + DLE+VA+HEIGHLLGL HSS+E +IMYP++  G  K  L  DD+EG++ 
Sbjct: 267 GDGFISVSEAVDLESVAVHEIGHLLGLGHSSVEGSIMYPTIRTGRRKVDLTTDDVEGVQY 326

[Close]
BLAST of Lsi02G015280 vs. TAIR10
Match: AT4G16640.1 (AT4G16640.1 Matrixin family protein)

HSP 1 Score: 125.6 bits (314), Expect = 4.4e-29
Identity = 64/119 (53.78%), Postives = 79/119 (66.39%), Query Frame = 1 

Query: 134 TFSQALDNQTSDIKIGFESGDHGDGYPFDGVGGVIAHAFSPPDGRFHLDAEESWAA---- 193
           +F +  D  T+D+KIGF +GDHGDG PFDGV G +AHAF+P +GR HLDA E+W      
Sbjct: 200 SFEEVDDFTTADLKIGFYAGDHGDGLPFDGVLGTLAHAFAPENGRLHLDAAETWIVDDDL 259

Query: 194 -GVISGSFDLETVALHEIGHLLGLQHSSIERAIMYPSV-PEGASKGLHADDIEGIKALY 247
            G    + DLE+VA HEIGHLLGL HSS E A+MYPS+ P      L  DD+ G+  LY
Sbjct: 260 KGSSEVAVDLESVATHEIGHLLGLGHSSQESAVMYPSLRPRTKKVDLTVDDVAGVLKLY 318

[Close]
BLAST of Lsi02G015280 vs. TAIR10
Match: AT2G45040.1 (AT2G45040.1 Matrixin family protein)

HSP 1 Score: 124.4 bits (311), Expect = 9.9e-29
Identity = 62/118 (52.54%), Postives = 82/118 (69.49%), Query Frame = 1 

Query: 134 TFSQALDNQTSDIKIGFESGDHGDGYPFDGVGGVIAHAFSPPDGRFHLDAEESWAAGV-- 193
           +F +  D   +DIKIGF +GDHGDG PFDGV GV+AH FSP +GR HLD  E+WA     
Sbjct: 178 SFIETEDYVIADIKIGFFNGDHGDGEPFDGVLGVLAHTFSPENGRLHLDKAETWAVDFDE 237

Query: 194 --ISGSFDLETVALHEIGHLLGLQHSSIERAIMYPSV-PEGASKGLHADDIEGIKALY 247
              S + DLE+VA+HEIGH+LGL HSS++ A MYP++ P      L+ DD+ G+++LY
Sbjct: 238 EKSSVAVDLESVAVHEIGHVLGLGHSSVKDAAMYPTLKPRSKKVNLNMDDVVGVQSLY 295

[Close]
BLAST of Lsi02G015280 vs. TAIR10
Match: AT1G59970.1 (AT1G59970.1 Matrixin family protein)

HSP 1 Score: 118.6 bits (296), Expect = 5.4e-27
Identity = 67/125 (53.60%), Postives = 83/125 (66.40%), Query Frame = 1 

Query: 132 HFTFSQALDNQTSDIKIGFESGDHGDGYPFDGVGGVIAHAFSPPDGRFHLDAEESW--AA 191
           +FT S+++    +DI IGF SG+HGDG PFDG  G +AHA SPP G  HLD +E W  + 
Sbjct: 191 NFTRSESI--LRADIVIGFFSGEHGDGEPFDGAMGTLAHASSPPTGMLHLDGDEDWLISN 250

Query: 192 GVISGSF-------DLETVALHEIGHLLGLQHSSIERAIMYPSVPEGASK-GLHADDIEG 247
           G IS          DLE+VA+HEIGHLLGL HSS+E AIM+P++  G  K  L  DDIEG
Sbjct: 251 GEISRRILPVTTVVDLESVAVHEIGHLLGLGHSSVEDAIMFPAISGGDRKVELAKDDIEG 310

[Close]
BLAST of Lsi02G015280 vs. NCBI nr
Match: gi|449449419|ref|XP_004142462.1| (PREDICTED: metalloendoproteinase 1 [Cucumis sativus])

HSP 1 Score: 236.5 bits (602), Expect = 5.0e-59
Identity = 125/156 (80.13%), Postives = 132/156 (84.62%), Query Frame = 1 

Query: 1   MALEELSFLTFTLLLLTFFPLISSSQPNSAHSHGKSPSQFVFLNQLHGSQKGDKVEGIHQ 60
           MALEELSF TFTLLLLTFFPLISSSQ N +HSHGK+ SQFVFLNQL+GSQKGDKVEGIHQ
Sbjct: 1   MALEELSFFTFTLLLLTFFPLISSSQSNHSHSHGKASSQFVFLNQLYGSQKGDKVEGIHQ 60

Query: 61  LKKYLHQFGYLN-DAQIHSENNDDEFDELLESAIKTYQKNYNLKVTGSLDAMTIAQMSKP 120
           LKKYLHQFGYLN DAQIHSENNDDEFDELLE+AIKTYQKNYNLKVTG+LDAMTIAQMSKP
Sbjct: 61  LKKYLHQFGYLNEDAQIHSENNDDEFDELLETAIKTYQKNYNLKVTGTLDAMTIAQMSKP 120

Query: 121 RCGVADIIHANSHFTFSQALDNQTSDIKIGFESGDH 156
           RCGVADIIH N+    S+        I   F S  H
Sbjct: 121 RCGVADIIHGNTWMRSSKKRKQLEHGIVGHFHSVSH 156

[Close]
BLAST of Lsi02G015280 vs. NCBI nr
Match: gi|595944536|ref|XP_007216020.1| (hypothetical protein PRUPE_ppa014830mg, partial [Prunus persica])

HSP 1 Score: 235.0 bits (598), Expect = 1.5e-58
Identity = 134/293 (45.73%), Postives = 172/293 (58.70%), Query Frame = 1 

Query: 2   ALEELSFLTFTLLLLTFFPLISSSQPNSAHSHGKSPSQFVFLNQLHGSQKGDKVEGIHQL 61
           ++  +  L+F+     F PLI  +   +      S S F FL  L G  KGDKV+GI  L
Sbjct: 5   SINGIKILSFSPPHPPFSPLIPCNFIRNPQQQNTS-SPFEFLEHLKGCHKGDKVQGIQDL 64

Query: 62  KKYLHQFGYLNDAQIHSENNDDEFDELLESAIKTYQKNYNLKVTGSLDAMTIAQMSKPRC 121
           KKYL +FGYL+    +   NDD+FD+ LESAIKTYQ NY+LK TG+LDA T++ M  PRC
Sbjct: 65  KKYLGKFGYLSSNN-NGHFNDDDFDDQLESAIKTYQINYHLKATGALDAKTVSNMMMPRC 124

Query: 122 GVADI---------------------IHANSHFTF------------------------- 181
           GVADI                      H  +H+TF                         
Sbjct: 125 GVADIINGTSSMRSGKQRHPHHHHHGGHTVAHYTFFRGNPKWPASKYHLTYAFLQDTPAE 184

Query: 182 SQALDNQTSDIKIGFESGDHGDGYPFDGVGGVIAHAFSPPDGRFHLDAEESWAAGVISGS 241
           +    NQ  D+ + F  G+HGDG PFDG GG IAHAF+P +GRFH DA+E ++ G +SG+
Sbjct: 185 ATGPSNQNPDLTVSFHRGNHGDGAPFDGPGGTIAHAFAPTNGRFHYDADERFSVGAVSGA 244

Query: 242 FDLETVALHEIGHLLGLQHSSIERAIMYPSVPEGAS-KGLHADDIEGIKALYN 248
           +DLETVALHEIGHLLGL HSS++ AIMYP++  G + + LH DDI+GIKALYN
Sbjct: 245 YDLETVALHEIGHLLGLGHSSVQGAIMYPTISPGVTQQSLHGDDIQGIKALYN 295

[Close]
BLAST of Lsi02G015280 vs. NCBI nr
Match: gi|659117648|ref|XP_008458714.1| (PREDICTED: metalloendoproteinase 1-like [Cucumis melo])

HSP 1 Score: 233.4 bits (594), Expect = 4.3e-58
Identity = 119/132 (90.15%), Postives = 123/132 (93.18%), Query Frame = 1 

Query: 1   MALEELSFLTFTLLLLTFFPLISSSQPNSAHSHGKSPSQFVFLNQLHGSQKGDKVEGIHQ 60
           MALEELSF   TLLLLTFFPLISSSQPN +HSHGK+ SQFVFLNQLHGSQKGDKVEGIHQ
Sbjct: 1   MALEELSFFALTLLLLTFFPLISSSQPNHSHSHGKASSQFVFLNQLHGSQKGDKVEGIHQ 60

Query: 61  LKKYLHQFGYLN-DAQIHSENNDDEFDELLESAIKTYQKNYNLKVTGSLDAMTIAQMSKP 120
           LKKYLHQFGYLN DAQIHSEN DDEFDELLESAIKTYQKNYNLKVTG+LDAMTIAQM KP
Sbjct: 61  LKKYLHQFGYLNEDAQIHSENKDDEFDELLESAIKTYQKNYNLKVTGTLDAMTIAQMLKP 120

Query: 121 RCGVADIIHANS 132
           RCGVADIIH N+
Sbjct: 121 RCGVADIIHGNT 132

[Close]
BLAST of Lsi02G015280 vs. NCBI nr
Match: gi|629082768|gb|KCW49213.1| (hypothetical protein EUGRSUZ_K02793, partial [Eucalyptus grandis])

HSP 1 Score: 213.8 bits (543), Expect = 3.5e-52
Identity = 120/236 (50.85%), Postives = 146/236 (61.86%), Query Frame = 1 

Query: 46  LHGSQKGDKVEGIHQLKKYLHQFGYLNDAQ-----IHSENNDDEFDELLESAIKTYQKNY 105
           L G  KGD   GI +LK+YL +FGYLN  Q     IH++  DD+FD+LLE A+KTYQ NY
Sbjct: 2   LQGCHKGDHTGGIRELKRYLEKFGYLNYNQSGHPTIHAD--DDDFDDLLEFAVKTYQLNY 61

Query: 106 NLKVTGSLDAMTIAQMSKPRCGVADIIHAN-------------------------SHFTF 165
           +L VTG+LD  T ++M +P    A   H                           + FTF
Sbjct: 62  HLNVTGTLDPQTTSKMMRPPRWPASNYHLTYGFLPGTPEAATAPVARAFAKWTSVTRFTF 121

Query: 166 SQALDNQTSDIKIGFESGDHGDGY--PFDGVGGVIAHAFSPPDGRFHLDAEESWAAGVIS 225
           S   D+Q ++I+IGF  GDHGDG    FDG GG +AHAF P  G FH DA+E WA    S
Sbjct: 122 SYVQDHQFANIQIGFARGDHGDGAHNSFDGPGGTLAHAFPPTSGLFHYDADEKWAVDPPS 181

Query: 226 GS--FDLETVALHEIGHLLGLQHSSIERAIMYPSVPEGASKGLHADDIEGIKALYN 248
           GS  F++ETVALHEIGHLLGL HS +E AIMY S+  G +KGLHADDI+GIKALYN
Sbjct: 182 GSAAFNVETVALHEIGHLLGLGHSDVEAAIMYSSIAPGTTKGLHADDIQGIKALYN 235

[Close]
BLAST of Lsi02G015280 vs. NCBI nr
Match: gi|590727367|ref|XP_007099566.1| (Matrix metalloproteinase, putative [Theobroma cacao])

HSP 1 Score: 200.7 bits (509), Expect = 3.1e-48
Identity = 104/204 (50.98%), Postives = 137/204 (67.16%), Query Frame = 1 

Query: 55  VEGIHQLKKYLHQFGYLNDAQIHSENNDDEFDELLESAIKTYQKNYNLKVTGSLDAMTIA 114
           V+ IH+LKKYL QF YL+ ++  +  NDD+FD+LLESAIKTYQ N++L    +LD  T++
Sbjct: 35  VKDIHKLKKYLEQFDYLSYSKNKTHANDDDFDDLLESAIKTYQLNFHLNSNRALDTETVS 94

Query: 115 QMSKPRCGVADIIH------------------ANSHFTFSQALDNQTSDIKIGFESGDHG 174
           +M  PR GVADII+                  AN++F FS+  + + ++I IGF+  DHG
Sbjct: 95  KMMMPRYGVADIINGTRVNVVSPVAGAFQTWAANTYFRFSRIYNYRDTNITIGFQRRDHG 154

Query: 175 DGYPFDGVGGVIAHAFSPPDGRFHLDAEESWAAGVISGSFDLETVALHEIGHLLGLQHSS 234
           DG PF+  GG IAHAF+P  GRF+ DA+E+W+     G+  LET+ALHEI HLLGL H+S
Sbjct: 155 DGNPFEDPGGTIAHAFAPTIGRFNYDADETWSVSARPGNMHLETIALHEIRHLLGLSHNS 214

Query: 235 IERAIMYPSVPEGASKGLHADDIE 241
           IE AIMYPS+ E  SK L  DDIE
Sbjct: 215 IENAIMYPSIAERTSKRLDRDDIE 238

The following BLAST results are available for this feature:
BLAST of Lsi02G015280 vs. Swiss-Prot
Match NameE-valueIdentityDescription
2MMP_ARATH1.2e-3156.10Metalloendoproteinase 2-MMP OS=Arabidopsis thaliana GN=2MMP PE=1 SV=1[more]
MEP1_SOYBN2.4e-2953.28Metalloendoproteinase 1 OS=Glycine max PE=1 SV=2[more]
3MMP_ARATH3.2e-2954.10Metalloendoproteinase 3-MMP OS=Arabidopsis thaliana GN=3MMP PE=1 SV=1[more]
1MMP_ARATH7.9e-2853.78Metalloendoproteinase 1-MMP OS=Arabidopsis thaliana GN=1MMP PE=1 SV=1[more]
4MMP_ARATH1.7e-2752.54Metalloendoproteinase 4-MMP OS=Arabidopsis thaliana GN=4MMP PE=1 SV=1[more]
BLAST of Lsi02G015280 vs. TrEMBL
Match NameE-valueIdentityDescription
A0A0A0KS39_CUCSA3.5e-5980.13Uncharacterized protein OS=Cucumis sativus GN=Csa_5G632120 PE=3 SV=1[more]
M5WU56_PRUPE1.0e-5845.73Uncharacterized protein (Fragment) OS=Prunus persica GN=PRUPE_ppa014830mg PE=3 S... [more]
A0A059A5V0_EUCGR2.4e-5250.85Uncharacterized protein (Fragment) OS=Eucalyptus grandis GN=EUGRSUZ_K02793 PE=3 ... [more]
S1S448_THECC2.1e-4850.98Matrix metalloproteinase, putative OS=Theobroma cacao GN=TCM_046070 PE=3 SV=1[more]
A0A151R3R2_CAJCA1.8e-4744.49Metalloendoproteinase 1 OS=Cajanus cajan GN=KK1_041636 PE=3 SV=1[more]
BLAST of Lsi02G015280 vs. TAIR10
Match NameE-valueIdentityDescription
AT1G70170.16.6e-3356.10 matrix metalloproteinase[more]
AT1G24140.11.8e-3054.10 Matrixin family protein[more]
AT4G16640.14.4e-2953.78 Matrixin family protein[more]
AT2G45040.19.9e-2952.54 Matrixin family protein[more]
AT1G59970.15.4e-2753.60 Matrixin family protein[more]
BLAST of Lsi02G015280 vs. NCBI nr
Match NameE-valueIdentityDescription
gi|449449419|ref|XP_004142462.1|5.0e-5980.13PREDICTED: metalloendoproteinase 1 [Cucumis sativus][more]
gi|595944536|ref|XP_007216020.1|1.5e-5845.73hypothetical protein PRUPE_ppa014830mg, partial [Prunus persica][more]
gi|659117648|ref|XP_008458714.1|4.3e-5890.15PREDICTED: metalloendoproteinase 1-like [Cucumis melo][more]
gi|629082768|gb|KCW49213.1|3.5e-5250.85hypothetical protein EUGRSUZ_K02793, partial [Eucalyptus grandis][more]
gi|590727367|ref|XP_007099566.1|3.1e-4850.98Matrix metalloproteinase, putative [Theobroma cacao][more]
Annotated Terms
The following terms have been associated with this gene:
Vocabulary: Molecular Function
TermDefinition
GO:0008237metallopeptidase activity
GO:0008270zinc ion binding
GO:0004222metalloendopeptidase activity
Vocabulary: Cellular Component
TermDefinition
GO:0031012extracellular matrix
Vocabulary: Biological Process
TermDefinition
GO:0006508proteolysis
Vocabulary: INTERPRO
TermDefinition
IPR024079MetalloPept_cat_dom_sf
IPR006026Peptidase_Metallo
IPR002477Peptidoglycan-bd-like
IPR001818Pept_M10_metallopeptidase
GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0006508 proteolysis
biological_process GO:0008150 biological_process
cellular_component GO:0031012 extracellular matrix
cellular_component GO:0005575 cellular_component
molecular_function GO:0005509 calcium ion binding
molecular_function GO:0004222 metalloendopeptidase activity
molecular_function GO:0008270 zinc ion binding
molecular_function GO:0046872 metal ion binding
molecular_function GO:0008237 metallopeptidase activity
Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Lsi02G015280.1Lsi02G015280.1mRNA


InterPro
Analysis Name: InterPro Annotations of Lagenaria siceraria
Date Performed: 2017-09-18
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR001818Peptidase M10, metallopeptidasePFAMPF00413Peptidase_M10coord: 129..246
score: 6.5
IPR002477Peptidoglycan binding-likeGENE3DG3DSA:1.10.101.10coord: 57..125
score: 2.3
IPR002477Peptidoglycan binding-likePFAMPF01471PG_binding_1coord: 56..116
score: 3.4
IPR002477Peptidoglycan binding-likeunknownSSF47090PGBD-likecoord: 50..126
score: 5.02
IPR006026Peptidase, metallopeptidaseSMARTSM00235col_5coord: 62..248
score: 6.2
IPR024079Metallopeptidase, catalytic domainGENE3DG3DSA:3.40.390.10coord: 131..247
score: 7.9
NoneNo IPR availablePANTHERPTHR10201MATRIX METALLOPROTEINASEcoord: 43..246
score: 1.4E-101coord: 4..25
score: 1.4E
NoneNo IPR availablePANTHERPTHR10201:SF140F20P5.11 PROTEINcoord: 43..246
score: 1.4E-101coord: 4..25
score: 1.4E
NoneNo IPR availableunknownSSF55486Metalloproteases ("zincins"), catalytic domaincoord: 124..247
score: 2.6

Synteny
The following gene(s) are orthologous to this gene:
GeneOrthologueOrganismBlock
Lsi02G015280CmaCh04G027320Cucurbita maxima (Rimu)cmalsiB651
The following gene(s) are paralogous to this gene:

None

The following block(s) are covering this gene:
GeneOrganismBlock
Lsi02G015280Cucumber (Gy14) v2cgyblsiB024
Lsi02G015280Cucumber (Gy14) v2cgyblsiB319
Lsi02G015280Melon (DHL92) v3.6.1lsimedB191
Lsi02G015280Melon (DHL92) v3.6.1lsimedB171
Lsi02G015280Silver-seed gourdcarlsiB232
Lsi02G015280Silver-seed gourdcarlsiB623
Lsi02G015280Cucumber (Chinese Long) v3cuclsiB028
Lsi02G015280Cucumber (Chinese Long) v3cuclsiB371
Lsi02G015280Watermelon (97103) v2lsiwmbB161
Lsi02G015280Watermelon (97103) v2lsiwmbB208
Lsi02G015280Wax gourdlsiwgoB207
Lsi02G015280Wax gourdlsiwgoB253
Lsi02G015280Bottle gourd (USVL1VR-Ls)lsilsiB089
Lsi02G015280Cucumber (Gy14) v1cgylsiB118
Lsi02G015280Cucumber (Gy14) v1cgylsiB251
Lsi02G015280Cucurbita maxima (Rimu)cmalsiB272
Lsi02G015280Cucurbita maxima (Rimu)cmalsiB372
Lsi02G015280Cucurbita moschata (Rifu)cmolsiB261
Lsi02G015280Cucurbita moschata (Rifu)cmolsiB640
Lsi02G015280Cucurbita pepo (Zucchini)cpelsiB141
Lsi02G015280Cucurbita pepo (Zucchini)cpelsiB336
Lsi02G015280Wild cucumber (PI 183967)cpilsiB028
Lsi02G015280Wild cucumber (PI 183967)cpilsiB352
Lsi02G015280Cucumber (Chinese Long) v2culsiB028
Lsi02G015280Cucumber (Chinese Long) v2culsiB348
Lsi02G015280Melon (DHL92) v3.5.1lsimeB162
Lsi02G015280Melon (DHL92) v3.5.1lsimeB182
Lsi02G015280Watermelon (Charleston Gray)lsiwcgB155
Lsi02G015280Watermelon (Charleston Gray)lsiwcgB176
Lsi02G015280Watermelon (97103) v1lsiwmB170