Lsi02G010170 (gene) Bottle gourd (USVL1VR-Ls)

BLAST of Lsi02G010170 vs. Swiss-Prot
Match: LIN2_LOTJA (Putative E3 ubiquitin-protein ligase LIN-2 OS=Lotus japonicus GN=LIN PE=1 SV=1)

HSP 1 Score: 224.6 bits (571), Expect = 5.0e-57
Identity = 160/528 (30.30%), Postives = 280/528 (53.03%), Query Frame = 1

Query: 459  NDFSKAITTICSSDILSECEIAIRVVTKAWLNAHGDTTIEVALSKPPVVEGMLEVLLASD 518
            N     I+++C+S+ L ECE A+  + +   ++  +  I   LSKP ++ G++E+L AS 
Sbjct: 658  NSLKPYISSLCTSENLPECEEAVLKIARLLKDSKTNPQIHSYLSKPTIINGLVEILSASR 717

Query: 519  DDEILELVISVLAELAVRSEVIRQMILNSDPQLQVFLKLLKSSSLFLKASILLYLSKPQA 578
            + E+L   I +L+EL    + + + + + D        LLK+     +A++L+Y  +P  
Sbjct: 718  NREVLRTSIYILSELIFTDDSVAETLNSVDSDFDCLATLLKNG--LAEAALLIYQLRPVF 777

Query: 579  KQMISVEWLPLVLRVLEFGGQLQTLFSVRCKPHEAAFYLLDQLLKGFDEDRNLENSRHLI 638
             Q+ + E +P ++ V++   +    F +   P +AA  +L+Q L G DE     N+  +I
Sbjct: 778  AQLSAHELIPSLVDVIQNKNEELDDFQLVIDPKDAAIAILEQTLMGGDEYSRSLNASSVI 837

Query: 639  SLGGLSLLLRRLERGEIEERKNSVSIILCCIQADGSCRNYLAENLNKASLLELIVHESNR 698
            S  G+  L++ LER  +E R++ VS++LCC+QA+ SC+N +A  +  + +LEL  H  N 
Sbjct: 838  SANGIPTLVKYLER--MEGRRSVVSVLLCCMQAEKSCKNLIANRIELSPVLELF-HSGND 897

Query: 699  NSDRCGLALLVDLLCLSRRTRITKLLDGLKEGWSGLGIMNILSVYLQRALPEEQPLVATI 758
            +     +  L +L+ L+RRT   +LL  +K+       M+   VYLQ A  E Q  VA++
Sbjct: 898  SVRGTCVEFLSELVQLNRRTSCNQLLHTIKDE-GAFSTMHTFLVYLQMAPMEHQLAVASL 957

Query: 759  QLQLDFMEDSLNCSIFREEAIVTIITALNARVCREKAQDNLARALLILGGRFSYAGEPST 818
             LQLD + +    SI+REEA+ T+I AL  +      Q     ALL L G  S +G+  T
Sbjct: 958  LLQLDLLAEPRKMSIYREEAVETLIEALWQKDF-SNTQMKALDALLFLIGHISSSGKSYT 1017

Query: 819  ENWLLQQAGFKENSGDSSHSKHL---YDDVVQLYEEEEGVVN-WQLKAATVLFNHGHKSL 878
            E WLL+ AGF +        + L    +D+++  E+E+  +N WQ + A+VL NH + S+
Sbjct: 1018 EAWLLKIAGFDQPYNALMKVEQLGQHDNDLIETMEDEKNALNSWQKRIASVLCNHENGSI 1077

Query: 879  LSALSTSMTSCIPSLAKASLITVSWMSRYLFVIKDEKLCLMAPSILVPPLIKYLNHDKDV 938
              AL   + S    +AK+ L+  +W++R L+ + D  +  +A   L+  +IK L+  K +
Sbjct: 1078 FKALEECLKSNSLKMAKSCLVLATWLTRMLYTLPDTGVRDVARKSLLEEVIKVLHSSKSL 1137

Query: 939  EDRVLASYSLFNLSKYTECTHIFRLFDEDSLDHLQNLSLVTWTAEELI 983
            ED +L + SL+          + R++ +     L+ L   +  A +++
Sbjct: 1138 EDMILVTLSLYPFISDPTVHEVLRVYAKSIYRILRKLKKYSTVAADIL 1178

BLAST of Lsi02G010170 vs. Swiss-Prot
Match: LIN1_LOTJA (Putative E3 ubiquitin-protein ligase LIN-1 OS=Lotus japonicus GN=CERBERUS PE=2 SV=2)

HSP 1 Score: 218.8 bits (556), Expect = 2.7e-55
Identity = 160/536 (29.85%), Postives = 284/536 (52.99%), Query Frame = 1

Query: 459  NDFSKAITTICSSDILSECEIAIRVVTKAWLNAHGDTTIEVALSKPPVVEGMLEVLLASD 518
            N     I+++C+S+ L ECE A+  + +   ++  +  I   LSKP ++ G++E+L AS 
Sbjct: 658  NSLKPYISSLCTSENLPECEEAVLKIARLLKDSKTNPQIHSYLSKPTIINGLVEILSASR 717

Query: 519  DDEILELVISVLAELAVRSEVIRQMILNSDPQLQVFLKLLKSSSLFLKASILLYLSKPQA 578
            + E+L   I +L+EL    + + + + + D        LLK+     +A++L+Y  +P  
Sbjct: 718  NREVLRTSIYILSELIFTDDSVAETLNSVDSDFDCLATLLKNG--LAEAALLIYQLRPVF 777

Query: 579  KQMISVEWLPLVLRVLEFGGQLQTLFSVRCKPHEAAFYLLDQLLKGFDEDRNLENSRHLI 638
             Q+ + E +P ++ V++   +    F +   P +AA  +L+Q L G DE     N+  +I
Sbjct: 778  AQLSAHELIPSLVDVIQNKNEELDDFQLVIDPKDAAIAILEQTLMGGDEYSRSLNASSVI 837

Query: 639  SLGGLSLLLRRLERGEIEERKNSVSIILCCIQADGSCRNYLAENLNKASLLELIVHESNR 698
            S  G+  L++ LER  +E R++ VS++LCC+QA+ SC+N +A  +  + +LEL  H  N 
Sbjct: 838  SANGIPTLVKYLER--MEGRRSVVSVLLCCMQAEKSCKNLIANRIELSPVLELF-HSGND 897

Query: 699  NSDRCGLALLVDLLCLSRRTRITKLLDGLKEGWSGLGIMNILSVYLQRALPEEQPLVATI 758
            +     +  L +L+ L+RRT   ++L  +K+       M+   VYLQ A  E Q  VA++
Sbjct: 898  SVRGTCVEFLSELVQLNRRTSCNQILHTIKDE-GAFSTMHTFLVYLQMAPMEHQLAVASL 957

Query: 759  QLQLDFMEDSLNCSIFREEAIVTIITALNARVCREKAQDNLARALLILGGRFSYAGEPST 818
             LQLD + +    SI+REEA+ T+I AL  +      Q     ALL L G  S +G+  T
Sbjct: 958  LLQLDLLAEPRKMSIYREEAVETLIEALWQKDF-SNTQMKALDALLFLIGHISSSGKSYT 1017

Query: 819  ENWLLQQAGFKENSGDSSHSKHLY---DDVVQLYEEEEGVVN-WQLKAATVLFNHGHKSL 878
            E WLL+ AGF +        + L    +D+++  E+E+  +N WQ + A+VL NH + S+
Sbjct: 1018 EAWLLKIAGFDQPYNALMKVEQLGQHDNDLIETMEDEKNALNSWQKRIASVLCNHENGSI 1077

Query: 879  LSALSTSMTSCIPSLAKASLITVSWMSRYLFVIKDEKLCLMAPSILVPPLIKYLNHDKDV 938
              AL   + S    +AK+ L+  +W++  L+ + D  +  +A   L+  +I  L   K++
Sbjct: 1078 FKALEECLKSNSLKMAKSCLVLATWLTHMLYTLPDTGVRDVARKSLLEEVINVLQSSKNL 1137

Query: 939  EDRVLASYSLFNLSKYTECTHIFRLFDED---SLDHLQNLSLVTWTAEELIIIVKS 988
            E+++LA+ +L             R++ +    +L  L+  S+V     ++I+ +KS
Sbjct: 1138 EEKILATLALKTFISDPSTHEALRVYAKSIYRTLRRLKKYSVVAVDIMKVILNLKS 1186

BLAST of Lsi02G010170 vs. Swiss-Prot
Match: LIN_MEDTR (Putative E3 ubiquitin-protein ligase LIN OS=Medicago truncatula GN=LIN PE=2 SV=1)

HSP 1 Score: 209.9 bits (533), Expect = 1.3e-52
Identity = 172/613 (28.06%), Postives = 303/613 (49.43%), Query Frame = 1

Query: 380  FSDKNYENSASIHDEHNNCRMSFVLDDRSHNTDSWHETVKSDYFRFFTCQNITKEYLESN 439
            FS+ N    +S      +  M   +      TDS  +  K DY R            + N
Sbjct: 591  FSNSNTPRGSSCSPSAKDITM---VSSIQRTTDSPSQKYKDDYIR------------QRN 650

Query: 440  NVITKNNF------VRVEGRNHLLSNDFSKAITTICSSDILSECEIAIRVVTKAWLNAHG 499
            N  T+ +       V  +     + N  +  IT++C+S+ L +CE A+  + + W ++  
Sbjct: 651  NRFTRVSVGASPTSVLSQAAVETIINSLTPYITSLCTSENLQDCEQAVLEIARLWKDSKT 710

Query: 500  DTTIEVALSKPPVVEGMLEVLLASDDDEILELVISVLAELAVRSEVIRQMILNSDPQLQV 559
            D  I   LSKP VV G++E+L AS + E+L   I +L+EL    E + + + + D     
Sbjct: 711  DPQIHSYLSKPTVVSGLVEILSASLNREVLRRSIYILSELIFSDERVGETLNSVDSDFDC 770

Query: 560  FLKLLKSSSLFLKASILLYLSKPQAKQMISVEWLPLVLRVLEFGGQLQTLFSVRCKPHEA 619
               LLK+     +A++L+Y  +P   Q+   E +P +++V++   +    F +   P  A
Sbjct: 771  LAMLLKNG--LAEAALLIYQLRPVFAQLSEHELIPSLIQVIQNKSEDIDDFQLAIDPKAA 830

Query: 620  AFYLLDQLLKGFDEDRNLENSRHLISLGGLSLLLRRLERGEIEERKNSVSIILCCIQADG 679
            A  +L+Q+L G DE     N+  +IS  G+  +++ L++   E R+  +SI+LCC+QA+ 
Sbjct: 831  AIAILEQILIGGDEYNRSVNASSVISANGIPAIVKYLDK--TEGRRPVISILLCCMQAEK 890

Query: 680  SCRNYLAENLNKASLLELIVHESNRNSDRCGLALLVDLLCLSRRTRITKLLDGLKEGWSG 739
            SC++ +A  +  + +LEL  H  N +     +  L +L+ L+RRT   + L  +K+    
Sbjct: 891  SCKSSIANRIELSPVLELF-HAGNDSVRGICVEFLSELVRLNRRTSSNQTLQIIKDE-GA 950

Query: 740  LGIMNILSVYLQRALPEEQPLVATIQLQLDFMEDSLNCSIFREEAIVTIITALNARVCRE 799
               M+   VYLQ A  E Q  VA++ LQLD + +    SI+REEA+ T+I AL  +    
Sbjct: 951  FSTMHTFLVYLQMAPMEHQIAVASLLLQLDLLAEPRKMSIYREEAVETLIEALWQKDFSN 1010

Query: 800  KAQDNLARALLILGGRFSYAGEPSTENWLLQQAGFKENSG---DSSHSKHLYDDVVQLYE 859
              Q     ALL L G  + +G+  TE  LL+ AGF +       +    H  +D ++  E
Sbjct: 1011 N-QMKALDALLFLIGHVTSSGKSYTEAGLLKIAGFDQPYNVLMKAEQLGHSDNDFMETME 1070

Query: 860  EEEGVV-NWQLKAATVLFNHGHKSLLSALSTSMTSCIPSLAKASLITVSWMSRYLFVIKD 919
            +E+  + +WQ + A+VL NH + S+  AL   + S    +AK+ L+  +W++  LF + D
Sbjct: 1071 DEKNAMKSWQKRVASVLCNHENGSIFQALEECLKSNSLKMAKSCLVLATWLTHMLFTLPD 1130

Query: 920  EKLCLMAPSILVPPLIKYLNHDKDVEDRVLASYSLFNLSKYTECTHIFRLFDEDSLDHLQ 979
              +  +A   L+  L+  L   K++E+++LAS +L +         + R++ +     L+
Sbjct: 1131 TGVRDVARKSLLEALMNVLQSSKNLEEKILASLALKSFISDPTVHEVLRVYAKSIYRILR 1181

Query: 980  NLSLVTWTAEELI 983
             L   +  A +++
Sbjct: 1191 KLKKYSTVAADIL 1181

BLAST of Lsi02G010170 vs. TrEMBL
Match: A0A0A0LYR5_CUCSA (Uncharacterized protein OS=Cucumis sativus GN=Csa_1G713660 PE=4 SV=1)

HSP 1 Score: 1764.2 bits (4568), Expect = 0.0e+00
Identity = 909/1008 (90.18%), Postives = 963/1008 (95.54%), Query Frame = 1

Query: 1    MASLQELLTREGFEGSSFPSTRKPTRLKGRSRATPDDSVTLPIYICHDKKIIDSSKKKAD 60
            MASLQELLTREGFEGS++PSTRK +R KGRSR  PDDSVTLPIYICHDKK IDSSKKK D
Sbjct: 1    MASLQELLTREGFEGSNYPSTRKLSRPKGRSRTAPDDSVTLPIYICHDKKTIDSSKKKHD 60

Query: 61   KPLIRNGSSVYSSKRVGSVSKTLLYKSMEEPTIDEIAIRAVVSILSGYVGRYSKDESFRE 120
            KPL+RNGSSVYSSKRVGSVS+TL  KSMEEP IDEIAIRAVVSILSGYVGRYSKDE+FRE
Sbjct: 61   KPLVRNGSSVYSSKRVGSVSETLPCKSMEEPAIDEIAIRAVVSILSGYVGRYSKDENFRE 120

Query: 121  ILRKKCNPCLIRKGEMESGIYSNLEMGMKSVDRLVEEGHGNERELRMKASRNSIGLLNMV 180
            I+RKKCNPCLIRKGEMESGI SNLEMGMKSVDRLVEEGHGNERELR+KASRNSIGLLNMV
Sbjct: 121  IVRKKCNPCLIRKGEMESGICSNLEMGMKSVDRLVEEGHGNERELRIKASRNSIGLLNMV 180

Query: 181  VTSLNSAKNSTKNGAHSHLSACAQLYLAIVYKIEKNEKVSAKHLLQVFCDSPFFARTHLL 240
            +TSL+SAKNSTKNGAHSHLSACAQLYLAIV KIEKNE+VSAKHLLQVFCDSP+FARTHLL
Sbjct: 181  ITSLDSAKNSTKNGAHSHLSACAQLYLAIVNKIEKNERVSAKHLLQVFCDSPYFARTHLL 240

Query: 241  PELWEHFFLPHLLHLKVWYNQELEFMSNFECEHKDRKIKALSKVYNEHMDRGTVQFALYY 300
            P+LWEHFFLPHLLHLKVWYNQELEF+SNFECEHKDRKIKAL+KVYN+HMDRGTVQFALYY
Sbjct: 241  PDLWEHFFLPHLLHLKVWYNQELEFVSNFECEHKDRKIKALNKVYNDHMDRGTVQFALYY 300

Query: 301  IQWLKDGAKAPPVPVVPSPSKSIYKASRRSSDSYFSQSSSNKNLYHAVFGPSLDQQLAEL 360
            IQWLKDGA+APPVPVVPSPSKSI+KASRRSSDSYFSQ SSNKNLYHAVFGPSLDQQLAEL
Sbjct: 301  IQWLKDGARAPPVPVVPSPSKSIHKASRRSSDSYFSQPSSNKNLYHAVFGPSLDQQLAEL 360

Query: 361  RSGNLVAAKARSSNEKEILFSDKNYENSASIHDEHNNCRMSFVLDDRSHNTDSWHETVKS 420
            R GNLVAA ARSS+EKEILFSDK+YENSAS+ DEH+N RMS VLD RSHNT+SW ETVKS
Sbjct: 361  RRGNLVAAMARSSHEKEILFSDKHYENSASVQDEHSNRRMSSVLDYRSHNTESWRETVKS 420

Query: 421  DYFRFFTCQNITKEYLESNNVITKNNFVRVEGRNHLLSNDFSKAITTICSSDILSECEIA 480
            DYFRFFTCQNITKEYLES+NVITKN+FV+VEGRNHLLSNDFSKAIT ICSSDILSECEIA
Sbjct: 421  DYFRFFTCQNITKEYLESSNVITKNSFVKVEGRNHLLSNDFSKAITAICSSDILSECEIA 480

Query: 481  IRVVTKAWLNAHGDTTIEVALSKPPVVEGMLEVLLASDDDEILELVISVLAELAVRSEVI 540
            +RVVTKAWL+AHGD+ IEVALS+PPVVEGMLEVLLASDDDEILELVISVLAELA RSE+I
Sbjct: 481  VRVVTKAWLDAHGDSNIEVALSQPPVVEGMLEVLLASDDDEILELVISVLAELAARSEII 540

Query: 541  RQMILNSDPQLQVFLKLLKSSSLFLKASILLYLSKPQAKQMISVEWLPLVLRVLEFGGQL 600
            +QMILNSDPQLQVFLKLLKSSSLFLKASILLYLSKPQAKQMISVEWLPLVLRVLEFGGQL
Sbjct: 541  QQMILNSDPQLQVFLKLLKSSSLFLKASILLYLSKPQAKQMISVEWLPLVLRVLEFGGQL 600

Query: 601  QTLFSVRCKPHEAAFYLLDQLLKGFDEDRNLENSRHLISLGGLSLLLRRLERGEIEERKN 660
            QTLFSV+CKPHEAAFYLLDQLLKGFDEDRNLENSRHLI+LGGLSLLLRRLERGEIEERKN
Sbjct: 601  QTLFSVQCKPHEAAFYLLDQLLKGFDEDRNLENSRHLIALGGLSLLLRRLERGEIEERKN 660

Query: 661  SVSIILCCIQADGSCRNYLAENLNKASLLELIVHESNRNSDRCGLALLVDLLCLSRRTRI 720
            SVSII CCIQADGSCRNYLAENLNKASLLEL+VHESN+NSDR GLALLVDLLCLSRRTRI
Sbjct: 661  SVSIISCCIQADGSCRNYLAENLNKASLLELVVHESNKNSDRGGLALLVDLLCLSRRTRI 720

Query: 721  TKLLDGLKEGWSGLGIMNILSVYLQRALPEEQPLVATIQLQLDFMEDSLNCSIFREEAIV 780
            TKLLDGLKEGWSGLGIMNILSVYLQRALPEEQPLVAT  LQLDFMED+LNCSIFREEAIV
Sbjct: 721  TKLLDGLKEGWSGLGIMNILSVYLQRALPEEQPLVATTLLQLDFMEDALNCSIFREEAIV 780

Query: 781  TIITALNARVCREKAQDNLARALLILGGRFSYAGEPSTENWLLQQAGFKENSGDSSHSKH 840
            TIITALNAR+C EKAQDNLARALLILGGRFS  GEPSTENWLL+ AGFKENSGDSSHSKH
Sbjct: 781  TIITALNARICGEKAQDNLARALLILGGRFSCTGEPSTENWLLKLAGFKENSGDSSHSKH 840

Query: 841  LYDDVVQLYEEEEGVVNWQLKAATVLFNHGHKSLLSALSTSMTSCI-PSLAKASLITVSW 900
            LYDDVVQLYEEEE V+NWQLKAA+VLFNHGHKSLLS+LSTSMTSCI PSLAKA LIT+SW
Sbjct: 841  LYDDVVQLYEEEEEVMNWQLKAASVLFNHGHKSLLSSLSTSMTSCIRPSLAKACLITLSW 900

Query: 901  MSRYLFVIKDEKLCLMAPSILVPPLIKYLNHDKDVEDRVLASYSLFNLSKYTECTHIFRL 960
            MSRYLFVI++EKLCLMAPSILVPPLIKYLNHDK VED+VLASYSL NL KYTEC HIFRL
Sbjct: 901  MSRYLFVIREEKLCLMAPSILVPPLIKYLNHDKAVEDQVLASYSLLNLGKYTECKHIFRL 960

Query: 961  FDEDSLDHLQNLSLVTWTAEELIIIVKSESMHSYTEQENSHTQRSIRK 1008
            FD+++LDHL+NLSLVTWTAEELI+I+ S SM  YTE+ENS  Q S R+
Sbjct: 961  FDDEALDHLRNLSLVTWTAEELILIITSGSMDPYTERENSDNQGSTRE 1008

BLAST of Lsi02G010170 vs. TrEMBL
Match: A0A061GC79_THECC (Transducin/WD40 repeat-like superfamily protein, putative OS=Theobroma cacao GN=TCM_029331 PE=4 SV=1)

HSP 1 Score: 1030.8 bits (2664), Expect = 1.1e-297
Identity = 566/982 (57.64%), Postives = 726/982 (73.93%), Query Frame = 1

Query: 2   ASLQELLTREGFEGSSFPSTRKPTRLKGRSRATPDDSVTLPIYICHDKKIIDSSKKKADK 61
           +SLQELLT EGFE        +  RL+ +SRA PD+SV LPIYICHD+K ++ SK +A+K
Sbjct: 3   SSLQELLTEEGFERGKSLKNPREVRLRNKSRA-PDESVALPIYICHDRKSLEKSKDEAEK 62

Query: 62  PLIRNGSSVYSSKRVGSV----SKTLLY---KSMEEPTIDEIAIRAVVSILSGYVGRYSK 121
            +IRNGSSV+SS+R+ S     SK+L+     + +EP ID++AIRAV+SIL GY+GRY K
Sbjct: 63  TVIRNGSSVFSSRRLSSSDRSKSKSLIKDGPSNRDEPPIDDVAIRAVISILGGYIGRYIK 122

Query: 122 DESFREILRKKCNPCLIR-KGEMESGIYSNLEMGMKSVDRLVEEGHGNERELRMKASRNS 181
           DESFRE++++KCN CL+R K   ++GI+ N+E+G++S+D+LVE+  GN++ELRMK+ RNS
Sbjct: 123 DESFREMIKEKCNSCLVRRKNGSDNGIFVNMELGIESIDKLVED-RGNKKELRMKSLRNS 182

Query: 182 IGLLNMVVTSLNSAKN---STKNGAHSHLSACAQLYLAIVYKIEKNEKVSAKHLLQVFCD 241
           I LL+ +V SLNS K+   ST    +SHLSACAQLYL+IVYK+EK +++SA+HLLQVFCD
Sbjct: 183 IRLLS-IVASLNSKKSRNGSTCGVPNSHLSACAQLYLSIVYKLEKTDRISARHLLQVFCD 242

Query: 242 SPFFARTHLLPELWEHFFLPHLLHLKVWYNQELEFMSNFECEHKDRKIKALSKVYNEHMD 301
           S F ARTHLLP+LWEHFFLPHLLHLKVWY++ELEF+SN E   K++++KALS++YN+ +D
Sbjct: 243 SAFLARTHLLPDLWEHFFLPHLLHLKVWYHKELEFLSNLEYGEKEKRMKALSELYNDQID 302

Query: 302 RGTVQFALYYIQWLKDGAKAPPVPVVPSPSKSIYKASRRSSDSYFSQSSSNKNLYHAVFG 361
            GTV+FA+YY +WLK GAKAP VP VP P+   Y    RSSDSY S SS NKNLY AVFG
Sbjct: 303 MGTVKFAMYYKEWLKIGAKAPAVPTVPLPTSPSY----RSSDSYASHSSINKNLYRAVFG 362

Query: 362 PSLDQQLAELRSGNLVAAKARSSNEKEILFSDKNYENSAS-IHDEHNNCRMSFVLDDRSH 421
            + ++Q  EL      +       E+E   +D  Y N  + +H++    R S     R+ 
Sbjct: 363 ATTERQSMELDHRIRASMDICRLEEEENECTDDEYYNGCNYVHNKTKTRRRS---STRTP 422

Query: 422 NTDSWHETVKSDYFRFFTCQNITKEYLESNNVITKNNFVRVEGRNHLLSNDFSKAITTIC 481
            T+SW ET KSD+FR FTCQ    E L +   + +NN ++ E + HL  +D SKAI TIC
Sbjct: 423 ETESWTETRKSDHFRLFTCQTGPTECLVNGKSVVRNNSMKKEEKVHLPMSDLSKAIATIC 482

Query: 482 SSDILSECEIAIRVVTKAWLNAHGDTTIEVALSKPPVVEGMLEVLLASDDDEILELVISV 541
           SSD LS+CEIAIRV+TKAWL +H D  +E AL+K PV+EG+LEVL AS DDEILEL IS+
Sbjct: 483 SSDSLSDCEIAIRVMTKAWLESHADPAVETALAKAPVIEGILEVLFASSDDEILELAISI 542

Query: 542 LAELAVRSEVIRQMILNSDPQLQVFLKLLKSSSLFLKASILLYLSKPQAKQMISVEWLPL 601
           LAE   R+EV RQM+LNSDPQL++FL+LL++SSLFLKA++LLYL KP+AKQMIS EW+PL
Sbjct: 543 LAEFVARNEVNRQMMLNSDPQLEIFLRLLRNSSLFLKAAVLLYLLKPKAKQMISTEWVPL 602

Query: 602 VLRVLEFGGQLQTLFSVRCKPHEAAFYLLDQLLKGFDEDRNLENSRHLISLGGLSLLLRR 661
           VLRVLE G QLQTLF+VRC P  AAFY LDQLL GF+EDRNLEN+  ++SLGGLSLL+R 
Sbjct: 603 VLRVLELGEQLQTLFTVRCSPQVAAFYFLDQLLTGFNEDRNLENATQVVSLGGLSLLIRN 662

Query: 662 LERGEIEERKNSVSIILCCIQADGSCRNYLAENLNKASLLELIVHESNRN-SDRCGLALL 721
            E G + ER N+  II CCI+ADGSCRNYLA+ LNKASL+ELIV  +NRN S+   +ALL
Sbjct: 663 FEIGGVLERNNAALIISCCIRADGSCRNYLADKLNKASLIELIV--ANRNDSNGTVVALL 722

Query: 722 VDLLCLSRRTRITKLLDGLKEGWSGLGIMNILSVYLQRALPEEQPLVATIQLQLDFMEDS 781
            +LLCL+RRT+ITK L+ L  GW GL   +IL   LQRALPEE+PLVA I LQLD + D 
Sbjct: 723 AELLCLNRRTQITKFLNDLLNGWRGLNTTHILLACLQRALPEERPLVAAILLQLDLLGDP 782

Query: 782 LNCSIFREEAIVTIITALNARVCREKAQDNLARALLILGGRFSYAGEPSTENWLLQQAGF 841
           L CS++REEA+  II AL+   C EK Q+  ARAL++LGGRFS  GE +TENWLLQQAGF
Sbjct: 783 LRCSVYREEAVEAIIEALDCEKCNEKIQEQSARALMMLGGRFSCMGEATTENWLLQQAGF 842

Query: 842 KENSGDSSHSKHLYDDVVQLYEEEEGVVNWQLKAATVLFNHGHKSLLSALSTSMTSCIPS 901
            E   DS HSK + DD+  L+EEEE +V+WQ KAA  L N G+K  L++LS SM   IPS
Sbjct: 843 HEKLEDSFHSKEIVDDI--LHEEEEAIVHWQRKAAIALLNSGNKRFLASLSNSMVKGIPS 902

Query: 902 LAKASLITVSWMSRYLFVIKDEKLCLMAPSILVPPLIKYLNHDKDVEDRVLASYSLFNLS 961
           LA+ASL+TV+WMS +L  ++D+    MA SILVP L++  N+++ +E+RVLAS+SL  L 
Sbjct: 903 LARASLLTVAWMSSFLHSVRDKDFQSMACSILVPQLLESSNYNQALEERVLASFSLQRLI 962

Query: 962 KYTECTHIFRLFDEDSLDHLQN 971
           K +E T I    DE  ++ L N
Sbjct: 963 KSSEYTSIISSLDETLVNPLSN 970

BLAST of Lsi02G010170 vs. TrEMBL
Match: F6HIT8_VITVI (Putative uncharacterized protein OS=Vitis vinifera GN=VIT_10s0042g01240 PE=4 SV=1)

HSP 1 Score: 1011.9 bits (2615), Expect = 5.4e-292
Identity = 563/1033 (54.50%), Postives = 735/1033 (71.15%), Query Frame = 1

Query: 1    MASLQELLTREGFEGS-SFPST-RKPTRLKGRSR---ATPDDSVTLPIYICHDKKIIDSS 60
            MASL +LL  EGFE + + P T RKP  L   +R      DDS+ LPIYICHD++   S 
Sbjct: 1    MASLHDLLVEEGFERTKNHPKTSRKPPLLSKPNRDPRLARDDSIALPIYICHDRRNFHSV 60

Query: 61   KKKADKPLIRNGSSVYSSKRVGSVSKTLLYKSM--------EEPTIDEIAIRAVVSILSG 120
            K KADK + RN   + SSKRV S S+    +S+        + P IDE+AIRAV+SILSG
Sbjct: 61   KHKADKAITRNAPGLLSSKRVSSDSERANSQSLGGSEGARRDGPAIDEVAIRAVISILSG 120

Query: 121  YVGRYSKDESFREILRKKCNPCL-IRKGEMESGIYSNLEMGMKSVDRLVEEGHGNERELR 180
            Y+GRY KDE+FRE +R+KC  CL  RK + ++G+++N+E+G++S+++LV    G   ELR
Sbjct: 121  YIGRYLKDETFRESVREKCYACLESRKKDSDNGVFANMELGIESIEQLVLGSPGTHMELR 180

Query: 181  MKASRNSIGLLNMVVTSLNSAKNSTKNGA-----HSHLSACAQLYLAIVYKIEKNEKVSA 240
            MK+ RNSI LL+ +V SLNS   +++NG+     +SHLSACAQLYL+IVYK+EKN+++SA
Sbjct: 181  MKSLRNSIRLLS-IVASLNS--ETSRNGSTCGIPNSHLSACAQLYLSIVYKLEKNDRISA 240

Query: 241  KHLLQVFCDSPFFARTHLLPELWEHFFLPHLLHLKVWYNQELEFMSNFECEHKDRKIKAL 300
            +HLLQVFCD+PF ART LLP+LWEHFFLPHLLHLKVWY  ELEF+SN     K+++  AL
Sbjct: 241  RHLLQVFCDAPFLARTDLLPDLWEHFFLPHLLHLKVWYANELEFLSNPNFGDKEKRAIAL 300

Query: 301  SKVYNEHMDRGTVQFALYYIQWLKDGAKAPPVPVVPSPSKSIYKAS-RRSSDSYFSQSSS 360
            SK+YN+ MD GT QFA YY  WLK G KAPP+P VP PS+  Y  S RRSSDS+ S  S 
Sbjct: 301  SKIYNDQMDMGTRQFAFYYKDWLKVGVKAPPIPSVPLPSRPSYGNSMRRSSDSFSSNLSI 360

Query: 361  NKNLYHAVFGPSLDQQLAELRSGNLVAAKARSSNEKEILFSDKNYENSASIHDEHNNC-- 420
            NKNLY AVFGP+ ++Q  E            S  EKE + +  N ++ A  H  HN    
Sbjct: 361  NKNLYQAVFGPTSERQSMEHSERTGAKIDTWSVEEKEKVCT--NEDSDARHHYVHNGLGA 420

Query: 421  -RMSFVLDDRSHNTDSWHETVKSDYFRFFTCQNITKEYLESNNVITKNNFVRVEGRNHLL 480
             R S     R    + W ET + D+FRFFTCQ    E L + N I +N+ +R E  ++L 
Sbjct: 421  QRRSPSQHYRFTKDELWSETQRIDFFRFFTCQRELTECLVNGNFIVRNDSIRKEENSYLP 480

Query: 481  SNDFSKAITTICSSDILSECEIAIRVVTKAWLNAHGDTTIEVALSKPPVVEGMLEVLLAS 540
            ++D ++AITTI SSD L++CE A+RV+TKAWL++HGD   E ALSK PV+EG+LEVL AS
Sbjct: 481  ASDLARAITTISSSDSLTDCERAVRVITKAWLDSHGDRVTESALSKAPVIEGILEVLFAS 540

Query: 541  DDDEILELVISVLAELAVRSEVIRQMILNSDPQLQVFLKLLKSSSLFLKASILLYLSKPQ 600
            +DDEILEL IS+LAE   R E  RQ+IL+SDPQL++F++LL+SSSLFLKA++LLYL KP+
Sbjct: 541  NDDEILELGISILAEFVWRKEANRQIILSSDPQLEIFMRLLRSSSLFLKAAVLLYLLKPK 600

Query: 601  AKQMISVEWLPLVLRVLEFGGQLQTLFSVRCKPHEAAFYLLDQLLKGFDEDRNLENSRHL 660
            AKQ+IS+EW+PLVLRVLEFG QLQTLF+VRC P  AA+Y LDQLL GF+ED+NLEN+R +
Sbjct: 601  AKQLISIEWIPLVLRVLEFGDQLQTLFTVRCSPQVAAYYFLDQLLMGFNEDQNLENARQV 660

Query: 661  ISLGGLSLLLRRLERGEIEERKNSVSIILCCIQADGSCRNYLAENLNKASLLELIVHESN 720
            +S+GGLSLL++R+E G+   R N+ SII CCIQADGSCR+YLA NLNKAS+LEL+V  + 
Sbjct: 661  VSIGGLSLLVKRIETGDACGRNNAASIISCCIQADGSCRHYLANNLNKASILELLVLGNQ 720

Query: 721  RNSDRCGLALLVDLLCLSRRTRITKLLDGLKEGWSGLGIMNILSVYLQRALPEEQPLVAT 780
            +NS  C  ALL +L+CL+RRT+ITK LDGL+ G + L  M+IL VYLQRA PEE+PLVA 
Sbjct: 721  KNSSSCAFALLTELICLNRRTQITKFLDGLQNGGAHLNTMHILLVYLQRAPPEERPLVAA 780

Query: 781  IQLQLDFMEDSLNCSIFREEAIVTIITALNARVCREKAQDNLARALLILGGRFSYAGEPS 840
            + LQLD + D    S++REEA+ TII AL+ + C EK Q   ++ L+ILGGRFSY GE S
Sbjct: 781  LLLQLDLLGDPSKSSVYREEAVETIIAALDCQTCNEKVQQQSSKTLMILGGRFSYTGEAS 840

Query: 841  TENWLLQQAGFKENSGDSSHSKHLYDDVVQ-----LYEEEEGVVNWQLKAATVLFNHGHK 900
             E WLLQQAG +E S DS H+  ++ + +        +EEE   NWQ KAA  LF  G+K
Sbjct: 841  AEKWLLQQAGLEEISEDSLHNTEIFVNEIMNSGSLENDEEEATENWQKKAAIALFRSGNK 900

Query: 901  SLLSALSTSMTSCIPSLAKASLITVSWMSRYLFVIKDEKLCLMAPSILVPPLIKYLNHDK 960
              LSALS S+ + IP LA+ASL+TVSWMS +L  ++DE    MA SILVP LI+ L++++
Sbjct: 901  RFLSALSDSIANGIPCLARASLVTVSWMSNFLCSMEDESFRWMACSILVPQLIELLSYNR 960

Query: 961  DVEDRVLASYSLFNLSKYTECTHIFRLFD-EDSLDHLQNLSLVTWTAEELIIIVKSESMH 1005
            DVE+RV+ASYSL NL+K +ECT +    D E+ ++ L+NLSLVTWTA EL+ I+ S   H
Sbjct: 961  DVEERVIASYSLLNLAKNSECTSMLSSLDHEELVNSLRNLSLVTWTANELMSIITSRPRH 1020

BLAST of Lsi02G010170 vs. TrEMBL
Match: A5ASG4_VITVI (Putative uncharacterized protein OS=Vitis vinifera GN=VITISV_004500 PE=4 SV=1)

HSP 1 Score: 1002.7 bits (2591), Expect = 3.3e-289
Identity = 563/1047 (53.77%), Postives = 735/1047 (70.20%), Query Frame = 1

Query: 1    MASLQELLTREGFEGS-SFPST-RKPTRLKGRSR---ATPDDSVTLPIYICHDKKIIDSS 60
            MASL +LL  EGFE + + P T RKP  L   +R      DDS+ LPIYICHD++   S 
Sbjct: 1    MASLHDLLVEEGFERTKNHPKTSRKPPLLSKPNRDPRLARDDSIALPIYICHDRRNFHSV 60

Query: 61   KKKADKPLIRNGSSVYSSKRVGSVSKTLLYKSM--------EEPTIDEIAIRAVVSILSG 120
            K KADK + RN   + SSKRV S S+    +S+        + P IDE+AIRAV+SILSG
Sbjct: 61   KHKADKAITRNAPGLLSSKRVSSDSERANSQSLGGSEGARRDGPAIDEVAIRAVISILSG 120

Query: 121  YVGRYSKDESFREILRKKCNPCL-IRKGEMESGIYSNLEMGMKSVDRLVEEGHGNERELR 180
            Y+GRY KDE+FRE +R+KC  CL  RK + ++G+++N+E+G++S+++LV    G   ELR
Sbjct: 121  YIGRYLKDETFRESVREKCYACLESRKKDSDNGVFANMELGIESIEQLVLGSPGTHMELR 180

Query: 181  MKASRNSIGLLNMVVTSLNSAKNSTKNGA-----HSHLSACAQLYLAIVYKIEKNEKVSA 240
            MK+ RNSI LL+ +V SLNS   +++NG+     +SHLSACAQLYL+IVYK+EKN+++SA
Sbjct: 181  MKSLRNSIRLLS-IVASLNS--ETSRNGSTCGIPNSHLSACAQLYLSIVYKLEKNDRISA 240

Query: 241  KHLLQVFCDSPFFARTHLLPELWEHFFLPHLLHLKVWYNQELEFMSNFECEHKDRKIKAL 300
            +HLLQVFCD+PF ART LLP+LWEHFFLPHLLHLKVWY  ELEF+SN     K+++  AL
Sbjct: 241  RHLLQVFCDAPFLARTDLLPDLWEHFFLPHLLHLKVWYANELEFLSNPNFGDKEKRAIAL 300

Query: 301  SKVYNEHMDRGTVQFALYYIQWLKDGAKAPPVPVVPSPSKSIYKAS-RRSSDSYFSQSSS 360
            SK+YN+ MD GT QFA YY  WLK G KAPP+P VP PS+  Y  S RRSSDS+ S  S 
Sbjct: 301  SKIYNDQMDMGTRQFAFYYKDWLKVGVKAPPIPSVPLPSRPSYGNSMRRSSDSFSSNLSI 360

Query: 361  NKNLYHAVFGPSLDQQLAELRSGNLVAAKARSSNEKEILFSDKNYENSASIHDEHNNC-- 420
            NKNLY AVFGP+ ++Q  E            S  EKE + +  N ++ A  H  HN    
Sbjct: 361  NKNLYQAVFGPTSERQSMEHSERTGAKIDTWSVEEKEKVCT--NEDSDARHHYVHNGLGA 420

Query: 421  -RMSFVLDDRSHNTDSWHETVKSDYFRFFTCQNITKEYLESNNVITKNNFVRVEGRNHLL 480
             R S     R    + W ET + D+FRFFTCQ    E L + N I +N+ +R E  ++L 
Sbjct: 421  QRRSPSQHYRFTKDELWSETQRIDFFRFFTCQRELTECLVNGNFIVRNDSIRKEENSYLP 480

Query: 481  SNDFSKAITTICSSDILSECEIAIRVVTKAWLNAHGDTTIEVALSKPPVVEGMLEVLLAS 540
            ++D ++AITTI SSD L++CE A+RV+TKAWL++HGD   E ALSK PV+EG+LEVL AS
Sbjct: 481  ASDLARAITTISSSDSLTDCERAVRVITKAWLDSHGDRVTESALSKAPVIEGILEVLFAS 540

Query: 541  DDDEILELVISVLAELAVRSEVIRQMILNSDPQLQVFLKLLKSSSLFLKASILLYLSKPQ 600
            +DDEILEL IS+LAE   R E  RQ+IL+SDPQL++F++LL+SSSLFLKA++LLYL KP+
Sbjct: 541  NDDEILELGISILAEFVWRKEANRQIILSSDPQLEIFMRLLRSSSLFLKAAVLLYLLKPK 600

Query: 601  AKQMISVEWLPLVLRVLEFGGQLQTLFSVRCKPHEAAFYLLDQLLKGFDEDRNLENSRHL 660
            AKQ+IS+EW+PLVLRVLEFG QLQTLF+VRC P  AA+Y LDQLL GF+ED+NLEN+R +
Sbjct: 601  AKQLISIEWIPLVLRVLEFGDQLQTLFTVRCSPQVAAYYFLDQLLMGFNEDQNLENARQV 660

Query: 661  ISLGGLSLLLRRLERGEIEERKNSVSIILCCIQADGSCRNYLAENLNKASLLELIVHESN 720
            +S+GGLSLL++R+E G+   R N+ SII CCIQADGSCR+YLA NLNKAS+LEL+V  + 
Sbjct: 661  VSIGGLSLLVKRIETGDACGRNNAASIISCCIQADGSCRHYLANNLNKASILELLVLGNQ 720

Query: 721  RNSDRCGLALLVDLLCLSRRTRITKLLDGLKEGWSGLGIMNILSVYLQRALPEEQPLVAT 780
            +NS  C  ALL +L+CL+RRT+ITK LDGL+ G + L  M+IL VYLQRA PEE+PLVA 
Sbjct: 721  KNSSSCAFALLTELICLNRRTQITKFLDGLQNGGAHLNTMHILLVYLQRAPPEERPLVAA 780

Query: 781  IQLQLDFME--------------DSLNCSIFREEAIVTIITALNARVCREKAQDNLARAL 840
            + LQLD +               D    S++REEA+ TII AL+ + C EK Q   ++ L
Sbjct: 781  LLLQLDLLTLEQPPHGVAVILQGDPSKSSVYREEAVETIIAALDCQTCNEKVQQQSSKTL 840

Query: 841  LILGGRFSYAGEPSTENWLLQQAGFKENSGDSSHSKHLYDDVVQ-----LYEEEEGVVNW 900
            +ILGGRFSY GE S E WLLQQAG +E S DS H+  ++ + +        +EEE   NW
Sbjct: 841  MILGGRFSYTGEASAEKWLLQQAGLEEISEDSLHNTEIFVNEIMNSGSLENDEEEATENW 900

Query: 901  QLKAATVLFNHGHKSLLSALSTSMTSCIPSLAKASLITVSWMSRYLFVIKDEKLCLMAPS 960
            Q KAA  LF  G+K  LSALS S+ + IP LA+ASL+TVSWMS +L  ++DE    MA S
Sbjct: 901  QKKAAIALFRSGNKRFLSALSDSIANGIPCLARASLVTVSWMSNFLCSMEDESFRWMACS 960

Query: 961  ILVPPLIKYLNHDKDVEDRVLASYSLFNLSKYTECTHIFRLFD-EDSLDHLQNLSLVTWT 1005
            ILVP LI+ L++++DVE+RV+ASYSL NL+K +ECT +    D E+ ++ L+NLSLVTWT
Sbjct: 961  ILVPQLIELLSYNRDVEERVIASYSLLNLAKNSECTSMLSSLDHEELVNSLRNLSLVTWT 1020

BLAST of Lsi02G010170 vs. TrEMBL
Match: M5WMF3_PRUPE (Uncharacterized protein OS=Prunus persica GN=PRUPE_ppa000859mg PE=4 SV=1)

HSP 1 Score: 986.1 bits (2548), Expect = 3.2e-284
Identity = 564/1006 (56.06%), Postives = 710/1006 (70.58%), Query Frame = 1

Query: 1   MASLQELLTREGFE-GSSFPSTRKPTRLKGRSRATPDDSVTL-PIYICHDKKIIDSSKKK 60
           M+SLQELLT E  E G  +P + KP  +K R R  PD+S+ L PIYICH +K  D S  +
Sbjct: 1   MSSLQELLTDERLELGKKYPKSPKP--VKHRERVAPDESIALLPIYICHGRKSYDFSNHE 60

Query: 61  ADKPLIRNGSSV-YSSKRVGSVSKTLLYKSME--EPTIDEIAIRAVVSILSGYVGRYSKD 120
           A KP +R GSS   SS    S SK+L+ +S    EP IDE+A RAV+SILSG  GRY KD
Sbjct: 61  AQKPAMRKGSSRRVSSTSERSNSKSLVSESSRTNEPAIDEVATRAVISILSGCAGRYIKD 120

Query: 121 ESFREILRKKCNPCLIRKGEMESG-IYSNLEMGMKSVDRLVEEGHGNERELRMKASRNSI 180
           E+FRE + +KC+ CL+RK + E   I++NLE+G++S+++LVE+   + +ELR K  RNSI
Sbjct: 121 EAFRETIWEKCSCCLVRKKKDEDNEIFANLELGIESINKLVEDQWSSNKELRKKTIRNSI 180

Query: 181 GLLNMVVTSLNSAKN---STKNGAHSHLSACAQLYLAIVYKIEKNEKVSAKHLLQVFCDS 240
            +L+ +V SLNS+K+   ST    +SHLSACAQLYLAI YKIEKN+ VSA+HLLQVFCDS
Sbjct: 181 RVLS-IVASLNSSKSKNGSTCGTPNSHLSACAQLYLAIAYKIEKNDPVSARHLLQVFCDS 240

Query: 241 PFFARTHLLPELWEHFFLPHLLHLKVWYNQELEFMSNFECEHKDRKIKALSKVYNEHMDR 300
           P  ARTHLLP+LWEHFFLPHLLH+K+WY +E + +SN E   +++K+KA++KVYN+ MD 
Sbjct: 241 PVLARTHLLPDLWEHFFLPHLLHVKIWYAREADVLSNSEDPEREKKMKAITKVYNDQMDM 300

Query: 301 GTVQFALYYIQWLKDGAKAPPV--PVVPSPSKSIYKASRR-SSDSYFSQSSSNKNLYHAV 360
           GT QFALYY +WLK G +APP   P +P PS S  ++SRR SSDSY S SS NKNLY A+
Sbjct: 301 GTTQFALYYKEWLKVGVEAPPPVPPNIPLPSISSCRSSRRRSSDSYTSHSSLNKNLYRAI 360

Query: 361 FGPSLDQQLAEL---RSGNLVAAKARSSNEKEILFSDKNYENSASIHDEHNNCRMSFVLD 420
           FGP+L+++  +L   R+G   A       E      + NY N + +H      R S   +
Sbjct: 361 FGPTLERRSLDLLYDRNGVSNATWGLHEEEGNQWADEDNYSNLSYVHRGGRTGRRSSSQN 420

Query: 421 DRSHNTDSWHE--TVKSDYFRFFTCQNITKEYLESNNVITKNNFVRVEGRNHLLSNDFSK 480
            R+  T+ W E  T KSDYF FF CQN   E L + N+I KNN +R E  +HL S++ S 
Sbjct: 421 HRNPKTEFWPEPETQKSDYFGFFRCQNGPTECLVNRNLIVKNNSIRKEDNSHLPSSNLSS 480

Query: 481 AITTICSSDILSECEIAIRVVTKAWLNAHGDTTIEVALSKPPVVEGMLEVLLASDDDEIL 540
           AI+TI SSD L +CEIAIRV+TKAWL++HGD  IE  L+K PV++GMLEVL  S DDEIL
Sbjct: 481 AISTIYSSDNLMDCEIAIRVITKAWLDSHGDPVIEAELAKAPVIQGMLEVLFVSTDDEIL 540

Query: 541 ELVISVLAELAVRSEVIRQMILNSDPQLQVFLKLLKSSSLFLKASILLYLSKPQAKQMIS 600
           ELVISVLAE   R+E+ R  ILNSDPQL++F++LL+SS LFLKA+ILLYL KP+AKQMIS
Sbjct: 541 ELVISVLAEFVARNEMNRHNILNSDPQLEIFMRLLRSSGLFLKAAILLYLLKPKAKQMIS 600

Query: 601 VEWLPLVLRVLEFGGQLQTLFSVRCKPHEAAFYLLDQLLKGFDEDRNLENSRHLISLGGL 660
           V+W+ LVLRVLEFG QLQTLF V+C P  AA YLLDQLL GFDEDRNLEN+R ++SLGGL
Sbjct: 601 VDWVALVLRVLEFGDQLQTLFRVQCSPQVAALYLLDQLLTGFDEDRNLENARQVVSLGGL 660

Query: 661 SLLLRRLERGEIEERKNSVSIILCCIQADGSCRNYLAENLNKASLLELIVHESNRNSDRC 720
           SLL+ ++ERG+  ER N  SII CC++ADGSCRNYLA+ LNKASLLELI+  +  NS   
Sbjct: 661 SLLVTQIERGDTHERNNIASIISCCVRADGSCRNYLADFLNKASLLELIILGNGSNSTGS 720

Query: 721 GLALLVDLLCLSRRTRITKLLDGLKEGWSGLGIMNILSVYLQRALPEEQPLVATIQLQLD 780
            +ALL+++LCLSRR +I ++LDGLKEG+ G   M IL V+LQRA PEE+PL+A I LQLD
Sbjct: 721 AVALLIEILCLSRRKKINEILDGLKEGYGGFNTMQILLVHLQRAAPEERPLIAAILLQLD 780

Query: 781 FMEDSLNCSIFREEAIVTIITALNARVCREKAQDNLARALLILGGRFSYAGEPSTENWLL 840
            M D    S++REEAI  II ALN + C EK Q+  A ALL+LGG FSY GE STE+ LL
Sbjct: 781 LMGDPFRSSVYREEAIEAIIAALNCQTCHEKVQERSASALLMLGGWFSYTGEASTEHRLL 840

Query: 841 QQAGFKENSGDSSHSKHLYDDVVQLYEEEEGVVNWQLKAATVLFNHGHKSLLSALSTSMT 900
           QQA                        + E   NWQ KAA VLF  G+K LL ALS S+ 
Sbjct: 841 QQA------------------------DGEATENWQRKAAIVLFKSGNKKLLVALSDSIA 900

Query: 901 SCIPSLAKASLITVSWMSRYLFVIKDEKLCLMAPSILVPPLIKYLNHDKDVEDRVLASYS 960
           + IPSLA+ASL+TVSWMS +L  + DE L  MA SILVP L++ LN+DKDVE+RVLASYS
Sbjct: 901 NGIPSLARASLVTVSWMSSFLSTVGDENLRNMACSILVPQLLESLNYDKDVEERVLASYS 960

Query: 961 LFNLSKYT--ECTHIFRLFDEDSLDHLQNLSLVTWTAEELIIIVKS 988
           L +L+K +  E   +    D++ +  L+NLSLVTWTA ELI I+ S
Sbjct: 961 LLSLAKSSAHEYVPMVSSLDKELVSKLKNLSLVTWTANELISIITS 979

BLAST of Lsi02G010170 vs. NCBI nr
Match: gi|778664640|ref|XP_004145484.2| (PREDICTED: putative E3 ubiquitin-protein ligase LIN-2 [Cucumis sativus])

HSP 1 Score: 1764.2 bits (4568), Expect = 0.0e+00
Identity = 909/1008 (90.18%), Postives = 963/1008 (95.54%), Query Frame = 1

Query: 1    MASLQELLTREGFEGSSFPSTRKPTRLKGRSRATPDDSVTLPIYICHDKKIIDSSKKKAD 60
            MASLQELLTREGFEGS++PSTRK +R KGRSR  PDDSVTLPIYICHDKK IDSSKKK D
Sbjct: 1    MASLQELLTREGFEGSNYPSTRKLSRPKGRSRTAPDDSVTLPIYICHDKKTIDSSKKKHD 60

Query: 61   KPLIRNGSSVYSSKRVGSVSKTLLYKSMEEPTIDEIAIRAVVSILSGYVGRYSKDESFRE 120
            KPL+RNGSSVYSSKRVGSVS+TL  KSMEEP IDEIAIRAVVSILSGYVGRYSKDE+FRE
Sbjct: 61   KPLVRNGSSVYSSKRVGSVSETLPCKSMEEPAIDEIAIRAVVSILSGYVGRYSKDENFRE 120

Query: 121  ILRKKCNPCLIRKGEMESGIYSNLEMGMKSVDRLVEEGHGNERELRMKASRNSIGLLNMV 180
            I+RKKCNPCLIRKGEMESGI SNLEMGMKSVDRLVEEGHGNERELR+KASRNSIGLLNMV
Sbjct: 121  IVRKKCNPCLIRKGEMESGICSNLEMGMKSVDRLVEEGHGNERELRIKASRNSIGLLNMV 180

Query: 181  VTSLNSAKNSTKNGAHSHLSACAQLYLAIVYKIEKNEKVSAKHLLQVFCDSPFFARTHLL 240
            +TSL+SAKNSTKNGAHSHLSACAQLYLAIV KIEKNE+VSAKHLLQVFCDSP+FARTHLL
Sbjct: 181  ITSLDSAKNSTKNGAHSHLSACAQLYLAIVNKIEKNERVSAKHLLQVFCDSPYFARTHLL 240

Query: 241  PELWEHFFLPHLLHLKVWYNQELEFMSNFECEHKDRKIKALSKVYNEHMDRGTVQFALYY 300
            P+LWEHFFLPHLLHLKVWYNQELEF+SNFECEHKDRKIKAL+KVYN+HMDRGTVQFALYY
Sbjct: 241  PDLWEHFFLPHLLHLKVWYNQELEFVSNFECEHKDRKIKALNKVYNDHMDRGTVQFALYY 300

Query: 301  IQWLKDGAKAPPVPVVPSPSKSIYKASRRSSDSYFSQSSSNKNLYHAVFGPSLDQQLAEL 360
            IQWLKDGA+APPVPVVPSPSKSI+KASRRSSDSYFSQ SSNKNLYHAVFGPSLDQQLAEL
Sbjct: 301  IQWLKDGARAPPVPVVPSPSKSIHKASRRSSDSYFSQPSSNKNLYHAVFGPSLDQQLAEL 360

Query: 361  RSGNLVAAKARSSNEKEILFSDKNYENSASIHDEHNNCRMSFVLDDRSHNTDSWHETVKS 420
            R GNLVAA ARSS+EKEILFSDK+YENSAS+ DEH+N RMS VLD RSHNT+SW ETVKS
Sbjct: 361  RRGNLVAAMARSSHEKEILFSDKHYENSASVQDEHSNRRMSSVLDYRSHNTESWRETVKS 420

Query: 421  DYFRFFTCQNITKEYLESNNVITKNNFVRVEGRNHLLSNDFSKAITTICSSDILSECEIA 480
            DYFRFFTCQNITKEYLES+NVITKN+FV+VEGRNHLLSNDFSKAIT ICSSDILSECEIA
Sbjct: 421  DYFRFFTCQNITKEYLESSNVITKNSFVKVEGRNHLLSNDFSKAITAICSSDILSECEIA 480

Query: 481  IRVVTKAWLNAHGDTTIEVALSKPPVVEGMLEVLLASDDDEILELVISVLAELAVRSEVI 540
            +RVVTKAWL+AHGD+ IEVALS+PPVVEGMLEVLLASDDDEILELVISVLAELA RSE+I
Sbjct: 481  VRVVTKAWLDAHGDSNIEVALSQPPVVEGMLEVLLASDDDEILELVISVLAELAARSEII 540

Query: 541  RQMILNSDPQLQVFLKLLKSSSLFLKASILLYLSKPQAKQMISVEWLPLVLRVLEFGGQL 600
            +QMILNSDPQLQVFLKLLKSSSLFLKASILLYLSKPQAKQMISVEWLPLVLRVLEFGGQL
Sbjct: 541  QQMILNSDPQLQVFLKLLKSSSLFLKASILLYLSKPQAKQMISVEWLPLVLRVLEFGGQL 600

Query: 601  QTLFSVRCKPHEAAFYLLDQLLKGFDEDRNLENSRHLISLGGLSLLLRRLERGEIEERKN 660
            QTLFSV+CKPHEAAFYLLDQLLKGFDEDRNLENSRHLI+LGGLSLLLRRLERGEIEERKN
Sbjct: 601  QTLFSVQCKPHEAAFYLLDQLLKGFDEDRNLENSRHLIALGGLSLLLRRLERGEIEERKN 660

Query: 661  SVSIILCCIQADGSCRNYLAENLNKASLLELIVHESNRNSDRCGLALLVDLLCLSRRTRI 720
            SVSII CCIQADGSCRNYLAENLNKASLLEL+VHESN+NSDR GLALLVDLLCLSRRTRI
Sbjct: 661  SVSIISCCIQADGSCRNYLAENLNKASLLELVVHESNKNSDRGGLALLVDLLCLSRRTRI 720

Query: 721  TKLLDGLKEGWSGLGIMNILSVYLQRALPEEQPLVATIQLQLDFMEDSLNCSIFREEAIV 780
            TKLLDGLKEGWSGLGIMNILSVYLQRALPEEQPLVAT  LQLDFMED+LNCSIFREEAIV
Sbjct: 721  TKLLDGLKEGWSGLGIMNILSVYLQRALPEEQPLVATTLLQLDFMEDALNCSIFREEAIV 780

Query: 781  TIITALNARVCREKAQDNLARALLILGGRFSYAGEPSTENWLLQQAGFKENSGDSSHSKH 840
            TIITALNAR+C EKAQDNLARALLILGGRFS  GEPSTENWLL+ AGFKENSGDSSHSKH
Sbjct: 781  TIITALNARICGEKAQDNLARALLILGGRFSCTGEPSTENWLLKLAGFKENSGDSSHSKH 840

Query: 841  LYDDVVQLYEEEEGVVNWQLKAATVLFNHGHKSLLSALSTSMTSCI-PSLAKASLITVSW 900
            LYDDVVQLYEEEE V+NWQLKAA+VLFNHGHKSLLS+LSTSMTSCI PSLAKA LIT+SW
Sbjct: 841  LYDDVVQLYEEEEEVMNWQLKAASVLFNHGHKSLLSSLSTSMTSCIRPSLAKACLITLSW 900

Query: 901  MSRYLFVIKDEKLCLMAPSILVPPLIKYLNHDKDVEDRVLASYSLFNLSKYTECTHIFRL 960
            MSRYLFVI++EKLCLMAPSILVPPLIKYLNHDK VED+VLASYSL NL KYTEC HIFRL
Sbjct: 901  MSRYLFVIREEKLCLMAPSILVPPLIKYLNHDKAVEDQVLASYSLLNLGKYTECKHIFRL 960

Query: 961  FDEDSLDHLQNLSLVTWTAEELIIIVKSESMHSYTEQENSHTQRSIRK 1008
            FD+++LDHL+NLSLVTWTAEELI+I+ S SM  YTE+ENS  Q S R+
Sbjct: 961  FDDEALDHLRNLSLVTWTAEELILIITSGSMDPYTERENSDNQGSTRE 1008

BLAST of Lsi02G010170 vs. NCBI nr
Match: gi|659118365|ref|XP_008459082.1| (PREDICTED: LOW QUALITY PROTEIN: putative E3 ubiquitin-protein ligase LIN-2 [Cucumis melo])

HSP 1 Score: 1741.1 bits (4508), Expect = 0.0e+00
Identity = 904/1008 (89.68%), Postives = 952/1008 (94.44%), Query Frame = 1

Query: 1    MASLQELLTREGFEGSSFPSTRKPTRLKGRSRATPDDSVTLPIYICHDKKIIDSSKKKAD 60
            MASLQELLTREGFEGS+FPS+RK +R KGRSR  PDDSVTLPIYICHDKK+IDSSKKK D
Sbjct: 1    MASLQELLTREGFEGSNFPSSRKLSRPKGRSRTAPDDSVTLPIYICHDKKMIDSSKKKLD 60

Query: 61   KPLIRNGSSVYSSKRVGSVSKTLLYKSMEEPTIDEIAIRAVVSILSGYVGRYSKDESFRE 120
            KPL+RNGSS+YSSKRVGS+S+T   KSMEEP ID+IAIRAVVSILSGYVGRYSKDE+FRE
Sbjct: 61   KPLVRNGSSIYSSKRVGSLSETFPCKSMEEPAIDDIAIRAVVSILSGYVGRYSKDENFRE 120

Query: 121  ILRKKCNPCLIRKGEMESGIYSNLEMGMKSVDRLVEEGHGNERELRMKASRNSIGLLNMV 180
            I+R KCNP LIRKGEMESGI SNLEMGMKSVDRLVEEGHGNERELR+KASRNSIGLLNMV
Sbjct: 121  IVRXKCNPYLIRKGEMESGICSNLEMGMKSVDRLVEEGHGNERELRIKASRNSIGLLNMV 180

Query: 181  VTSLNSAKNSTKNGAHSHLSACAQLYLAIVYKIEKNEKVSAKHLLQVFCDSPFFARTHLL 240
            +TSL+SA   TKNGA+SHLSACA LYLAIV KIEK EK+SAKHLLQVFCDSPFFARTHLL
Sbjct: 181  ITSLDSAIKFTKNGAYSHLSACAHLYLAIVNKIEKKEKISAKHLLQVFCDSPFFARTHLL 240

Query: 241  PELWEHFFLPHLLHLKVWYNQELEFMSNFECEHKDRKIKALSKVYNEHMDRGTVQFALYY 300
            P+LWEHFFLPHLLHLKVWYNQELEF+SNFECEHKDRK KAL+KVYN+HMDRGTVQFALYY
Sbjct: 241  PDLWEHFFLPHLLHLKVWYNQELEFVSNFECEHKDRKTKALNKVYNDHMDRGTVQFALYY 300

Query: 301  IQWLKDGAKAPPVPVVPSPSKSIYKASRRSSDSYFSQSSSNKNLYHAVFGPSLDQQLAEL 360
            IQWLKDGA+APPVPVV SPSKSI+KASRRSSDSYFSQ SSNKNLYHAVFGPSLDQQLA L
Sbjct: 301  IQWLKDGARAPPVPVVRSPSKSIHKASRRSSDSYFSQPSSNKNLYHAVFGPSLDQQLAGL 360

Query: 361  RSGNLVAAKARSSNEKEILFSDKNYENSASIHDEHNNCRMSFVLDDRSHNTDSWHETVKS 420
            RSGNLVAA ARSSNEKEILFSDK+YENSAS+ DEH+N RMS VLD RSHNT+SW ETVKS
Sbjct: 361  RSGNLVAALARSSNEKEILFSDKHYENSASLQDEHSNRRMSSVLDYRSHNTESWRETVKS 420

Query: 421  DYFRFFTCQNITKEYLESNNVITKNNFVRVEGRNHLLSNDFSKAITTICSSDILSECEIA 480
            DYFRFFTCQ+ITKEYLES+NVITKN+ VRVEGRNHLLSND SKAIT ICSSDILSECEIA
Sbjct: 421  DYFRFFTCQSITKEYLESSNVITKNSSVRVEGRNHLLSNDLSKAITAICSSDILSECEIA 480

Query: 481  IRVVTKAWLNAHGDTTIEVALSKPPVVEGMLEVLLASDDDEILELVISVLAELAVRSEVI 540
            IRVVTKAWL+AHGDTTIEVALSKPPVVEGMLEVLLASDDDEILELVIS LAELAVRSEVI
Sbjct: 481  IRVVTKAWLDAHGDTTIEVALSKPPVVEGMLEVLLASDDDEILELVISFLAELAVRSEVI 540

Query: 541  RQMILNSDPQLQVFLKLLKSSSLFLKASILLYLSKPQAKQMISVEWLPLVLRVLEFGGQL 600
            RQMILNSDPQLQVFLKLLKSSSLFLKASILLYLSKPQAKQMISVEWLPLVLRVLEFGGQL
Sbjct: 541  RQMILNSDPQLQVFLKLLKSSSLFLKASILLYLSKPQAKQMISVEWLPLVLRVLEFGGQL 600

Query: 601  QTLFSVRCKPHEAAFYLLDQLLKGFDEDRNLENSRHLISLGGLSLLLRRLERGEIEERKN 660
            QTLFSVRCKPHEAAFYLLDQLLKGFDEDRNLENSRHLISLGGLSLLLRRLERGEIEERKN
Sbjct: 601  QTLFSVRCKPHEAAFYLLDQLLKGFDEDRNLENSRHLISLGGLSLLLRRLERGEIEERKN 660

Query: 661  SVSIILCCIQADGSCRNYLAENLNKASLLELIVHESNRNSDRCGLALLVDLLCLSRRTRI 720
            SVSII CC+QADGSCRNYLAENLNKASLLELIVHESN+NS R GLALLVDLLCLSRRTRI
Sbjct: 661  SVSIISCCVQADGSCRNYLAENLNKASLLELIVHESNKNSGRGGLALLVDLLCLSRRTRI 720

Query: 721  TKLLDGLKEGWSGLGIMNILSVYLQRALPEEQPLVATIQLQLDFMEDSLNCSIFREEAIV 780
            TKLLDGLKEGWSGLGIMNILSVYLQRALPEEQPLVAT+ LQLDFMED+LNCSIFREEAIV
Sbjct: 721  TKLLDGLKEGWSGLGIMNILSVYLQRALPEEQPLVATMLLQLDFMEDALNCSIFREEAIV 780

Query: 781  TIITALNARVCREKAQDNLARALLILGGRFSYAGEPSTENWLLQQAGFKENSGDSSHSKH 840
            TIITALNAR+  EK QDNLARALLILGGRFS  GEPSTENWLL+ AGF+ENSGDSSHSKH
Sbjct: 781  TIITALNARISGEKVQDNLARALLILGGRFSCTGEPSTENWLLKLAGFRENSGDSSHSKH 840

Query: 841  LYDDVVQLYEEEEGVVNWQLKAATVLFNHGHKSLLSALSTSMTSCI-PSLAKASLITVSW 900
            LYDDVV LYEEEE VVNWQLKAATVLFNHGHKSLLS+LSTSMTSC+ PSLAKA LIT+SW
Sbjct: 841  LYDDVVLLYEEEEEVVNWQLKAATVLFNHGHKSLLSSLSTSMTSCVRPSLAKACLITLSW 900

Query: 901  MSRYLFVIKDEKLCLMAPSILVPPLIKYLNHDKDVEDRVLASYSLFNLSKYTECTHIFRL 960
            MSRYLFVI+DEKLCLMAPSILVP LIKYLNHDK VED+VLASYSL NL KYTEC HIFRL
Sbjct: 901  MSRYLFVIRDEKLCLMAPSILVPALIKYLNHDKAVEDQVLASYSLLNLGKYTECKHIFRL 960

Query: 961  FDEDSLDHLQNLSLVTWTAEELIIIVKSESMHSYTEQENSHTQRSIRK 1008
            FDE++LDHL+NLSLVTWTAEELI+I+ S SMH +TEQENSHTQ S RK
Sbjct: 961  FDEEALDHLRNLSLVTWTAEELILIITSGSMHPHTEQENSHTQGSTRK 1008

BLAST of Lsi02G010170 vs. NCBI nr
Match: gi|590621804|ref|XP_007024873.1| (Transducin/WD40 repeat-like superfamily protein, putative [Theobroma cacao])

HSP 1 Score: 1030.8 bits (2664), Expect = 1.6e-297
Identity = 566/982 (57.64%), Postives = 726/982 (73.93%), Query Frame = 1

Query: 2   ASLQELLTREGFEGSSFPSTRKPTRLKGRSRATPDDSVTLPIYICHDKKIIDSSKKKADK 61
           +SLQELLT EGFE        +  RL+ +SRA PD+SV LPIYICHD+K ++ SK +A+K
Sbjct: 3   SSLQELLTEEGFERGKSLKNPREVRLRNKSRA-PDESVALPIYICHDRKSLEKSKDEAEK 62

Query: 62  PLIRNGSSVYSSKRVGSV----SKTLLY---KSMEEPTIDEIAIRAVVSILSGYVGRYSK 121
            +IRNGSSV+SS+R+ S     SK+L+     + +EP ID++AIRAV+SIL GY+GRY K
Sbjct: 63  TVIRNGSSVFSSRRLSSSDRSKSKSLIKDGPSNRDEPPIDDVAIRAVISILGGYIGRYIK 122

Query: 122 DESFREILRKKCNPCLIR-KGEMESGIYSNLEMGMKSVDRLVEEGHGNERELRMKASRNS 181
           DESFRE++++KCN CL+R K   ++GI+ N+E+G++S+D+LVE+  GN++ELRMK+ RNS
Sbjct: 123 DESFREMIKEKCNSCLVRRKNGSDNGIFVNMELGIESIDKLVED-RGNKKELRMKSLRNS 182

Query: 182 IGLLNMVVTSLNSAKN---STKNGAHSHLSACAQLYLAIVYKIEKNEKVSAKHLLQVFCD 241
           I LL+ +V SLNS K+   ST    +SHLSACAQLYL+IVYK+EK +++SA+HLLQVFCD
Sbjct: 183 IRLLS-IVASLNSKKSRNGSTCGVPNSHLSACAQLYLSIVYKLEKTDRISARHLLQVFCD 242

Query: 242 SPFFARTHLLPELWEHFFLPHLLHLKVWYNQELEFMSNFECEHKDRKIKALSKVYNEHMD 301
           S F ARTHLLP+LWEHFFLPHLLHLKVWY++ELEF+SN E   K++++KALS++YN+ +D
Sbjct: 243 SAFLARTHLLPDLWEHFFLPHLLHLKVWYHKELEFLSNLEYGEKEKRMKALSELYNDQID 302

Query: 302 RGTVQFALYYIQWLKDGAKAPPVPVVPSPSKSIYKASRRSSDSYFSQSSSNKNLYHAVFG 361
            GTV+FA+YY +WLK GAKAP VP VP P+   Y    RSSDSY S SS NKNLY AVFG
Sbjct: 303 MGTVKFAMYYKEWLKIGAKAPAVPTVPLPTSPSY----RSSDSYASHSSINKNLYRAVFG 362

Query: 362 PSLDQQLAELRSGNLVAAKARSSNEKEILFSDKNYENSAS-IHDEHNNCRMSFVLDDRSH 421
            + ++Q  EL      +       E+E   +D  Y N  + +H++    R S     R+ 
Sbjct: 363 ATTERQSMELDHRIRASMDICRLEEEENECTDDEYYNGCNYVHNKTKTRRRS---STRTP 422

Query: 422 NTDSWHETVKSDYFRFFTCQNITKEYLESNNVITKNNFVRVEGRNHLLSNDFSKAITTIC 481
            T+SW ET KSD+FR FTCQ    E L +   + +NN ++ E + HL  +D SKAI TIC
Sbjct: 423 ETESWTETRKSDHFRLFTCQTGPTECLVNGKSVVRNNSMKKEEKVHLPMSDLSKAIATIC 482

Query: 482 SSDILSECEIAIRVVTKAWLNAHGDTTIEVALSKPPVVEGMLEVLLASDDDEILELVISV 541
           SSD LS+CEIAIRV+TKAWL +H D  +E AL+K PV+EG+LEVL AS DDEILEL IS+
Sbjct: 483 SSDSLSDCEIAIRVMTKAWLESHADPAVETALAKAPVIEGILEVLFASSDDEILELAISI 542

Query: 542 LAELAVRSEVIRQMILNSDPQLQVFLKLLKSSSLFLKASILLYLSKPQAKQMISVEWLPL 601
           LAE   R+EV RQM+LNSDPQL++FL+LL++SSLFLKA++LLYL KP+AKQMIS EW+PL
Sbjct: 543 LAEFVARNEVNRQMMLNSDPQLEIFLRLLRNSSLFLKAAVLLYLLKPKAKQMISTEWVPL 602

Query: 602 VLRVLEFGGQLQTLFSVRCKPHEAAFYLLDQLLKGFDEDRNLENSRHLISLGGLSLLLRR 661
           VLRVLE G QLQTLF+VRC P  AAFY LDQLL GF+EDRNLEN+  ++SLGGLSLL+R 
Sbjct: 603 VLRVLELGEQLQTLFTVRCSPQVAAFYFLDQLLTGFNEDRNLENATQVVSLGGLSLLIRN 662

Query: 662 LERGEIEERKNSVSIILCCIQADGSCRNYLAENLNKASLLELIVHESNRN-SDRCGLALL 721
            E G + ER N+  II CCI+ADGSCRNYLA+ LNKASL+ELIV  +NRN S+   +ALL
Sbjct: 663 FEIGGVLERNNAALIISCCIRADGSCRNYLADKLNKASLIELIV--ANRNDSNGTVVALL 722

Query: 722 VDLLCLSRRTRITKLLDGLKEGWSGLGIMNILSVYLQRALPEEQPLVATIQLQLDFMEDS 781
            +LLCL+RRT+ITK L+ L  GW GL   +IL   LQRALPEE+PLVA I LQLD + D 
Sbjct: 723 AELLCLNRRTQITKFLNDLLNGWRGLNTTHILLACLQRALPEERPLVAAILLQLDLLGDP 782

Query: 782 LNCSIFREEAIVTIITALNARVCREKAQDNLARALLILGGRFSYAGEPSTENWLLQQAGF 841
           L CS++REEA+  II AL+   C EK Q+  ARAL++LGGRFS  GE +TENWLLQQAGF
Sbjct: 783 LRCSVYREEAVEAIIEALDCEKCNEKIQEQSARALMMLGGRFSCMGEATTENWLLQQAGF 842

Query: 842 KENSGDSSHSKHLYDDVVQLYEEEEGVVNWQLKAATVLFNHGHKSLLSALSTSMTSCIPS 901
            E   DS HSK + DD+  L+EEEE +V+WQ KAA  L N G+K  L++LS SM   IPS
Sbjct: 843 HEKLEDSFHSKEIVDDI--LHEEEEAIVHWQRKAAIALLNSGNKRFLASLSNSMVKGIPS 902

Query: 902 LAKASLITVSWMSRYLFVIKDEKLCLMAPSILVPPLIKYLNHDKDVEDRVLASYSLFNLS 961
           LA+ASL+TV+WMS +L  ++D+    MA SILVP L++  N+++ +E+RVLAS+SL  L 
Sbjct: 903 LARASLLTVAWMSSFLHSVRDKDFQSMACSILVPQLLESSNYNQALEERVLASFSLQRLI 962

Query: 962 KYTECTHIFRLFDEDSLDHLQN 971
           K +E T I    DE  ++ L N
Sbjct: 963 KSSEYTSIISSLDETLVNPLSN 970

BLAST of Lsi02G010170 vs. NCBI nr
Match: gi|731405827|ref|XP_002274319.3| (PREDICTED: putative E3 ubiquitin-protein ligase LIN [Vitis vinifera])

HSP 1 Score: 1011.9 bits (2615), Expect = 7.7e-292
Identity = 563/1033 (54.50%), Postives = 735/1033 (71.15%), Query Frame = 1

Query: 1    MASLQELLTREGFEGS-SFPST-RKPTRLKGRSR---ATPDDSVTLPIYICHDKKIIDSS 60
            MASL +LL  EGFE + + P T RKP  L   +R      DDS+ LPIYICHD++   S 
Sbjct: 2    MASLHDLLVEEGFERTKNHPKTSRKPPLLSKPNRDPRLARDDSIALPIYICHDRRNFHSV 61

Query: 61   KKKADKPLIRNGSSVYSSKRVGSVSKTLLYKSM--------EEPTIDEIAIRAVVSILSG 120
            K KADK + RN   + SSKRV S S+    +S+        + P IDE+AIRAV+SILSG
Sbjct: 62   KHKADKAITRNAPGLLSSKRVSSDSERANSQSLGGSEGARRDGPAIDEVAIRAVISILSG 121

Query: 121  YVGRYSKDESFREILRKKCNPCL-IRKGEMESGIYSNLEMGMKSVDRLVEEGHGNERELR 180
            Y+GRY KDE+FRE +R+KC  CL  RK + ++G+++N+E+G++S+++LV    G   ELR
Sbjct: 122  YIGRYLKDETFRESVREKCYACLESRKKDSDNGVFANMELGIESIEQLVLGSPGTHMELR 181

Query: 181  MKASRNSIGLLNMVVTSLNSAKNSTKNGA-----HSHLSACAQLYLAIVYKIEKNEKVSA 240
            MK+ RNSI LL+ +V SLNS   +++NG+     +SHLSACAQLYL+IVYK+EKN+++SA
Sbjct: 182  MKSLRNSIRLLS-IVASLNS--ETSRNGSTCGIPNSHLSACAQLYLSIVYKLEKNDRISA 241

Query: 241  KHLLQVFCDSPFFARTHLLPELWEHFFLPHLLHLKVWYNQELEFMSNFECEHKDRKIKAL 300
            +HLLQVFCD+PF ART LLP+LWEHFFLPHLLHLKVWY  ELEF+SN     K+++  AL
Sbjct: 242  RHLLQVFCDAPFLARTDLLPDLWEHFFLPHLLHLKVWYANELEFLSNPNFGDKEKRAIAL 301

Query: 301  SKVYNEHMDRGTVQFALYYIQWLKDGAKAPPVPVVPSPSKSIYKAS-RRSSDSYFSQSSS 360
            SK+YN+ MD GT QFA YY  WLK G KAPP+P VP PS+  Y  S RRSSDS+ S  S 
Sbjct: 302  SKIYNDQMDMGTRQFAFYYKDWLKVGVKAPPIPSVPLPSRPSYGNSMRRSSDSFSSNLSI 361

Query: 361  NKNLYHAVFGPSLDQQLAELRSGNLVAAKARSSNEKEILFSDKNYENSASIHDEHNNC-- 420
            NKNLY AVFGP+ ++Q  E            S  EKE + +  N ++ A  H  HN    
Sbjct: 362  NKNLYQAVFGPTSERQSMEHSERTGAKIDTWSVEEKEKVCT--NEDSDARHHYVHNGLGA 421

Query: 421  -RMSFVLDDRSHNTDSWHETVKSDYFRFFTCQNITKEYLESNNVITKNNFVRVEGRNHLL 480
             R S     R    + W ET + D+FRFFTCQ    E L + N I +N+ +R E  ++L 
Sbjct: 422  QRRSPSQHYRFTKDELWSETQRIDFFRFFTCQRELTECLVNGNFIVRNDSIRKEENSYLP 481

Query: 481  SNDFSKAITTICSSDILSECEIAIRVVTKAWLNAHGDTTIEVALSKPPVVEGMLEVLLAS 540
            ++D ++AITTI SSD L++CE A+RV+TKAWL++HGD   E ALSK PV+EG+LEVL AS
Sbjct: 482  ASDLARAITTISSSDSLTDCERAVRVITKAWLDSHGDRVTESALSKAPVIEGILEVLFAS 541

Query: 541  DDDEILELVISVLAELAVRSEVIRQMILNSDPQLQVFLKLLKSSSLFLKASILLYLSKPQ 600
            +DDEILEL IS+LAE   R E  RQ+IL+SDPQL++F++LL+SSSLFLKA++LLYL KP+
Sbjct: 542  NDDEILELGISILAEFVWRKEANRQIILSSDPQLEIFMRLLRSSSLFLKAAVLLYLLKPK 601

Query: 601  AKQMISVEWLPLVLRVLEFGGQLQTLFSVRCKPHEAAFYLLDQLLKGFDEDRNLENSRHL 660
            AKQ+IS+EW+PLVLRVLEFG QLQTLF+VRC P  AA+Y LDQLL GF+ED+NLEN+R +
Sbjct: 602  AKQLISIEWIPLVLRVLEFGDQLQTLFTVRCSPQVAAYYFLDQLLMGFNEDQNLENARQV 661

Query: 661  ISLGGLSLLLRRLERGEIEERKNSVSIILCCIQADGSCRNYLAENLNKASLLELIVHESN 720
            +S+GGLSLL++R+E G+   R N+ SII CCIQADGSCR+YLA NLNKAS+LEL+V  + 
Sbjct: 662  VSIGGLSLLVKRIETGDACGRNNAASIISCCIQADGSCRHYLANNLNKASILELLVLGNQ 721

Query: 721  RNSDRCGLALLVDLLCLSRRTRITKLLDGLKEGWSGLGIMNILSVYLQRALPEEQPLVAT 780
            +NS  C  ALL +L+CL+RRT+ITK LDGL+ G + L  M+IL VYLQRA PEE+PLVA 
Sbjct: 722  KNSSSCAFALLTELICLNRRTQITKFLDGLQNGGAHLNTMHILLVYLQRAPPEERPLVAA 781

Query: 781  IQLQLDFMEDSLNCSIFREEAIVTIITALNARVCREKAQDNLARALLILGGRFSYAGEPS 840
            + LQLD + D    S++REEA+ TII AL+ + C EK Q   ++ L+ILGGRFSY GE S
Sbjct: 782  LLLQLDLLGDPSKSSVYREEAVETIIAALDCQTCNEKVQQQSSKTLMILGGRFSYTGEAS 841

Query: 841  TENWLLQQAGFKENSGDSSHSKHLYDDVVQ-----LYEEEEGVVNWQLKAATVLFNHGHK 900
             E WLLQQAG +E S DS H+  ++ + +        +EEE   NWQ KAA  LF  G+K
Sbjct: 842  AEKWLLQQAGLEEISEDSLHNTEIFVNEIMNSGSLENDEEEATENWQKKAAIALFRSGNK 901

Query: 901  SLLSALSTSMTSCIPSLAKASLITVSWMSRYLFVIKDEKLCLMAPSILVPPLIKYLNHDK 960
              LSALS S+ + IP LA+ASL+TVSWMS +L  ++DE    MA SILVP LI+ L++++
Sbjct: 902  RFLSALSDSIANGIPCLARASLVTVSWMSNFLCSMEDESFRWMACSILVPQLIELLSYNR 961

Query: 961  DVEDRVLASYSLFNLSKYTECTHIFRLFD-EDSLDHLQNLSLVTWTAEELIIIVKSESMH 1005
            DVE+RV+ASYSL NL+K +ECT +    D E+ ++ L+NLSLVTWTA EL+ I+ S   H
Sbjct: 962  DVEERVIASYSLLNLAKNSECTSMLSSLDHEELVNSLRNLSLVTWTANELMSIITSRPRH 1021

BLAST of Lsi02G010170 vs. NCBI nr
Match: gi|147794281|emb|CAN67079.1| (hypothetical protein VITISV_004500 [Vitis vinifera])

HSP 1 Score: 1002.7 bits (2591), Expect = 4.7e-289
Identity = 563/1047 (53.77%), Postives = 735/1047 (70.20%), Query Frame = 1

Query: 1    MASLQELLTREGFEGS-SFPST-RKPTRLKGRSR---ATPDDSVTLPIYICHDKKIIDSS 60
            MASL +LL  EGFE + + P T RKP  L   +R      DDS+ LPIYICHD++   S 
Sbjct: 1    MASLHDLLVEEGFERTKNHPKTSRKPPLLSKPNRDPRLARDDSIALPIYICHDRRNFHSV 60

Query: 61   KKKADKPLIRNGSSVYSSKRVGSVSKTLLYKSM--------EEPTIDEIAIRAVVSILSG 120
            K KADK + RN   + SSKRV S S+    +S+        + P IDE+AIRAV+SILSG
Sbjct: 61   KHKADKAITRNAPGLLSSKRVSSDSERANSQSLGGSEGARRDGPAIDEVAIRAVISILSG 120

Query: 121  YVGRYSKDESFREILRKKCNPCL-IRKGEMESGIYSNLEMGMKSVDRLVEEGHGNERELR 180
            Y+GRY KDE+FRE +R+KC  CL  RK + ++G+++N+E+G++S+++LV    G   ELR
Sbjct: 121  YIGRYLKDETFRESVREKCYACLESRKKDSDNGVFANMELGIESIEQLVLGSPGTHMELR 180

Query: 181  MKASRNSIGLLNMVVTSLNSAKNSTKNGA-----HSHLSACAQLYLAIVYKIEKNEKVSA 240
            MK+ RNSI LL+ +V SLNS   +++NG+     +SHLSACAQLYL+IVYK+EKN+++SA
Sbjct: 181  MKSLRNSIRLLS-IVASLNS--ETSRNGSTCGIPNSHLSACAQLYLSIVYKLEKNDRISA 240

Query: 241  KHLLQVFCDSPFFARTHLLPELWEHFFLPHLLHLKVWYNQELEFMSNFECEHKDRKIKAL 300
            +HLLQVFCD+PF ART LLP+LWEHFFLPHLLHLKVWY  ELEF+SN     K+++  AL
Sbjct: 241  RHLLQVFCDAPFLARTDLLPDLWEHFFLPHLLHLKVWYANELEFLSNPNFGDKEKRAIAL 300

Query: 301  SKVYNEHMDRGTVQFALYYIQWLKDGAKAPPVPVVPSPSKSIYKAS-RRSSDSYFSQSSS 360
            SK+YN+ MD GT QFA YY  WLK G KAPP+P VP PS+  Y  S RRSSDS+ S  S 
Sbjct: 301  SKIYNDQMDMGTRQFAFYYKDWLKVGVKAPPIPSVPLPSRPSYGNSMRRSSDSFSSNLSI 360

Query: 361  NKNLYHAVFGPSLDQQLAELRSGNLVAAKARSSNEKEILFSDKNYENSASIHDEHNNC-- 420
            NKNLY AVFGP+ ++Q  E            S  EKE + +  N ++ A  H  HN    
Sbjct: 361  NKNLYQAVFGPTSERQSMEHSERTGAKIDTWSVEEKEKVCT--NEDSDARHHYVHNGLGA 420

Query: 421  -RMSFVLDDRSHNTDSWHETVKSDYFRFFTCQNITKEYLESNNVITKNNFVRVEGRNHLL 480
             R S     R    + W ET + D+FRFFTCQ    E L + N I +N+ +R E  ++L 
Sbjct: 421  QRRSPSQHYRFTKDELWSETQRIDFFRFFTCQRELTECLVNGNFIVRNDSIRKEENSYLP 480

Query: 481  SNDFSKAITTICSSDILSECEIAIRVVTKAWLNAHGDTTIEVALSKPPVVEGMLEVLLAS 540
            ++D ++AITTI SSD L++CE A+RV+TKAWL++HGD   E ALSK PV+EG+LEVL AS
Sbjct: 481  ASDLARAITTISSSDSLTDCERAVRVITKAWLDSHGDRVTESALSKAPVIEGILEVLFAS 540

Query: 541  DDDEILELVISVLAELAVRSEVIRQMILNSDPQLQVFLKLLKSSSLFLKASILLYLSKPQ 600
            +DDEILEL IS+LAE   R E  RQ+IL+SDPQL++F++LL+SSSLFLKA++LLYL KP+
Sbjct: 541  NDDEILELGISILAEFVWRKEANRQIILSSDPQLEIFMRLLRSSSLFLKAAVLLYLLKPK 600

Query: 601  AKQMISVEWLPLVLRVLEFGGQLQTLFSVRCKPHEAAFYLLDQLLKGFDEDRNLENSRHL 660
            AKQ+IS+EW+PLVLRVLEFG QLQTLF+VRC P  AA+Y LDQLL GF+ED+NLEN+R +
Sbjct: 601  AKQLISIEWIPLVLRVLEFGDQLQTLFTVRCSPQVAAYYFLDQLLMGFNEDQNLENARQV 660

Query: 661  ISLGGLSLLLRRLERGEIEERKNSVSIILCCIQADGSCRNYLAENLNKASLLELIVHESN 720
            +S+GGLSLL++R+E G+   R N+ SII CCIQADGSCR+YLA NLNKAS+LEL+V  + 
Sbjct: 661  VSIGGLSLLVKRIETGDACGRNNAASIISCCIQADGSCRHYLANNLNKASILELLVLGNQ 720

Query: 721  RNSDRCGLALLVDLLCLSRRTRITKLLDGLKEGWSGLGIMNILSVYLQRALPEEQPLVAT 780
            +NS  C  ALL +L+CL+RRT+ITK LDGL+ G + L  M+IL VYLQRA PEE+PLVA 
Sbjct: 721  KNSSSCAFALLTELICLNRRTQITKFLDGLQNGGAHLNTMHILLVYLQRAPPEERPLVAA 780

Query: 781  IQLQLDFME--------------DSLNCSIFREEAIVTIITALNARVCREKAQDNLARAL 840
            + LQLD +               D    S++REEA+ TII AL+ + C EK Q   ++ L
Sbjct: 781  LLLQLDLLTLEQPPHGVAVILQGDPSKSSVYREEAVETIIAALDCQTCNEKVQQQSSKTL 840

Query: 841  LILGGRFSYAGEPSTENWLLQQAGFKENSGDSSHSKHLYDDVVQ-----LYEEEEGVVNW 900
            +ILGGRFSY GE S E WLLQQAG +E S DS H+  ++ + +        +EEE   NW
Sbjct: 841  MILGGRFSYTGEASAEKWLLQQAGLEEISEDSLHNTEIFVNEIMNSGSLENDEEEATENW 900

Query: 901  QLKAATVLFNHGHKSLLSALSTSMTSCIPSLAKASLITVSWMSRYLFVIKDEKLCLMAPS 960
            Q KAA  LF  G+K  LSALS S+ + IP LA+ASL+TVSWMS +L  ++DE    MA S
Sbjct: 901  QKKAAIALFRSGNKRFLSALSDSIANGIPCLARASLVTVSWMSNFLCSMEDESFRWMACS 960

Query: 961  ILVPPLIKYLNHDKDVEDRVLASYSLFNLSKYTECTHIFRLFD-EDSLDHLQNLSLVTWT 1005
            ILVP LI+ L++++DVE+RV+ASYSL NL+K +ECT +    D E+ ++ L+NLSLVTWT
Sbjct: 961  ILVPQLIELLSYNRDVEERVIASYSLLNLAKNSECTSMLSSLDHEELVNSLRNLSLVTWT 1020