Cp4.1LG20g05970 (gene) Cucurbita pepo (Zucchini)

BLAST of Cp4.1LG20g05970 vs. Swiss-Prot
Match: LIN2_LOTJA (Putative E3 ubiquitin-protein ligase LIN-2 OS=Lotus japonicus GN=LIN PE=1 SV=1)

HSP 1 Score: 211.8 bits (538), Expect = 3.1e-53
Identity = 159/529 (30.06%), Postives = 276/529 (52.17%), Query Frame = 1

Query: 377  NELCKAISTICSSDILSECEIAIRVVSKAWLDVDGDTTIEVALSTPPFVEGMLEVLLASN 436
            N L   IS++C+S+ L ECE A+  +++   D   +  I   LS P  + G++E+L AS 
Sbjct: 658  NSLKPYISSLCTSENLPECEEAVLKIARLLKDSKTNPQIHSYLSKPTIINGLVEILSASR 717

Query: 437  DDEILELVISILAELAARSEAIRLMILNSDPQLQVFLKLLKSSSLFLKASILLYLSKPQA 496
            + E+L   I IL+EL    +++   + + D        LLK+     +A++L+Y  +P  
Sbjct: 718  NREVLRTSIYILSELIFTDDSVAETLNSVDSDFDCLATLLKNG--LAEAALLIYQLRPVF 777

Query: 497  KQMISVEWLPLVLRVLEFGGSLQMLFSVRCAPYEAAFYLLDQLLKGFDEDRNLENGRHLI 556
             Q+ + E +P ++ V++        F +   P +AA  +L+Q L G DE     N   +I
Sbjct: 778  AQLSAHELIPSLVDVIQNKNEELDDFQLVIDPKDAAIAILEQTLMGGDEYSRSLNASSVI 837

Query: 557  ALGGLSLLLRTLENGEIEERKNTVSMISCCIRADGTCRNYLAENLNKAALLELVVHESNK 616
            +  G+  L++ LE   +E R++ VS++ CC++A+ +C+N +A  +  + +LEL  H  N 
Sbjct: 838  SANGIPTLVKYLE--RMEGRRSVVSVLLCCMQAEKSCKNLIANRIELSPVLEL-FHSGND 897

Query: 617  NSDRCGLALLVELLCLSRRTRITKLLDELKERSNGSSIMNILSVDLHRARPEEQPLVATI 676
            +     +  L EL+ L+RRT   +LL  +K+     S M+   V L  A  E Q  VA++
Sbjct: 898  SVRGTCVEFLSELVQLNRRTSCNQLLHTIKD-EGAFSTMHTFLVYLQMAPMEHQLAVASL 957

Query: 677  LLQLDLMDDPLNCCSIFREEAIVTIITALNSQISREKTSENLARALLILGGRFSCTGEPC 736
            LLQLDL+ +P    SI+REEA+ T+I AL  Q     T      ALL L G  S +G+  
Sbjct: 958  LLQLDLLAEPRK-MSIYREEAVETLIEAL-WQKDFSNTQMKALDALLFLIGHISSSGKSY 1017

Query: 737  PEIWLLQQAGFET--NSGDSSYSSRHVYDDLVQSYEEEEDLVN-WQLKASTVFFNHGHKS 796
             E WLL+ AGF+   N+           +DL+++ E+E++ +N WQ + ++V  NH + S
Sbjct: 1018 TEAWLLKIAGFDQPYNALMKVEQLGQHDNDLIETMEDEKNALNSWQKRIASVLCNHENGS 1077

Query: 797  LLSALSTSMTNGIPRLAKASLVTVSWMSRYLFVIRDEKLCSMAPSILVPSLIKYLNYDKD 856
            +  AL   + +   ++AK+ LV  +W++R L+ + D  +  +A   L+  +IK L+  K 
Sbjct: 1078 IFKALEECLKSNSLKMAKSCLVLATWLTRMLYTLPDTGVRDVARKSLLEEVIKVLHSSKS 1137

Query: 857  VEDRVLASYTLLNLIKYTECKHIFGLFDEEARDHLENLSLVTWTAEELI 903
            +ED +L + +L   I       +  ++ +     L  L   +  A +++
Sbjct: 1138 LEDMILVTLSLYPFISDPTVHEVLRVYAKSIYRILRKLKKYSTVAADIL 1178

BLAST of Cp4.1LG20g05970 vs. Swiss-Prot
Match: LIN1_LOTJA (Putative E3 ubiquitin-protein ligase LIN-1 OS=Lotus japonicus GN=CERBERUS PE=2 SV=2)

HSP 1 Score: 208.8 bits (530), Expect = 2.6e-52
Identity = 156/532 (29.32%), Postives = 276/532 (51.88%), Query Frame = 1

Query: 377  NELCKAISTICSSDILSECEIAIRVVSKAWLDVDGDTTIEVALSTPPFVEGMLEVLLASN 436
            N L   IS++C+S+ L ECE A+  +++   D   +  I   LS P  + G++E+L AS 
Sbjct: 658  NSLKPYISSLCTSENLPECEEAVLKIARLLKDSKTNPQIHSYLSKPTIINGLVEILSASR 717

Query: 437  DDEILELVISILAELAARSEAIRLMILNSDPQLQVFLKLLKSSSLFLKASILLYLSKPQA 496
            + E+L   I IL+EL    +++   + + D        LLK+     +A++L+Y  +P  
Sbjct: 718  NREVLRTSIYILSELIFTDDSVAETLNSVDSDFDCLATLLKNG--LAEAALLIYQLRPVF 777

Query: 497  KQMISVEWLPLVLRVLEFGGSLQMLFSVRCAPYEAAFYLLDQLLKGFDEDRNLENGRHLI 556
             Q+ + E +P ++ V++        F +   P +AA  +L+Q L G DE     N   +I
Sbjct: 778  AQLSAHELIPSLVDVIQNKNEELDDFQLVIDPKDAAIAILEQTLMGGDEYSRSLNASSVI 837

Query: 557  ALGGLSLLLRTLENGEIEERKNTVSMISCCIRADGTCRNYLAENLNKAALLELVVHESNK 616
            +  G+  L++ LE   +E R++ VS++ CC++A+ +C+N +A  +  + +LEL  H  N 
Sbjct: 838  SANGIPTLVKYLER--MEGRRSVVSVLLCCMQAEKSCKNLIANRIELSPVLEL-FHSGND 897

Query: 617  NSDRCGLALLVELLCLSRRTRITKLLDELKERSNGSSIMNILSVDLHRARPEEQPLVATI 676
            +     +  L EL+ L+RRT   ++L  +K+     S M+   V L  A  E Q  VA++
Sbjct: 898  SVRGTCVEFLSELVQLNRRTSCNQILHTIKD-EGAFSTMHTFLVYLQMAPMEHQLAVASL 957

Query: 677  LLQLDLMDDPLNCCSIFREEAIVTIITALNSQISREKTSENLARALLILGGRFSCTGEPC 736
            LLQLDL+ +P    SI+REEA+ T+I AL  Q     T      ALL L G  S +G+  
Sbjct: 958  LLQLDLLAEPRK-MSIYREEAVETLIEAL-WQKDFSNTQMKALDALLFLIGHISSSGKSY 1017

Query: 737  PEIWLLQQAGFET--NSGDSSYSSRHVYDDLVQSYEEEEDLVN-WQLKASTVFFNHGHKS 796
             E WLL+ AGF+   N+           +DL+++ E+E++ +N WQ + ++V  NH + S
Sbjct: 1018 TEAWLLKIAGFDQPYNALMKVEQLGQHDNDLIETMEDEKNALNSWQKRIASVLCNHENGS 1077

Query: 797  LLSALSTSMTNGIPRLAKASLVTVSWMSRYLFVIRDEKLCSMAPSILVPSLIKYLNYDKD 856
            +  AL   + +   ++AK+ LV  +W++  L+ + D  +  +A   L+  +I  L   K+
Sbjct: 1078 IFKALEECLKSNSLKMAKSCLVLATWLTHMLYTLPDTGVRDVARKSLLEEVINVLQSSKN 1137

Query: 857  VEDRVLASYTLLNLIKYTECKHIFGLFDEEARDHLENLSLVTWTAEELISIV 906
            +E+++LA+  L   I          ++ +     L  L   +  A +++ ++
Sbjct: 1138 LEEKILATLALKTFISDPSTHEALRVYAKSIYRTLRRLKKYSVVAVDIMKVI 1181

BLAST of Cp4.1LG20g05970 vs. Swiss-Prot
Match: LIN_MEDTR (Putative E3 ubiquitin-protein ligase LIN OS=Medicago truncatula GN=LIN PE=2 SV=1)

HSP 1 Score: 199.5 bits (506), Expect = 1.6e-49
Identity = 152/529 (28.73%), Postives = 273/529 (51.61%), Query Frame = 1

Query: 377  NELCKAISTICSSDILSECEIAIRVVSKAWLDVDGDTTIEVALSTPPFVEGMLEVLLASN 436
            N L   I+++C+S+ L +CE A+  +++ W D   D  I   LS P  V G++E+L AS 
Sbjct: 661  NSLTPYITSLCTSENLQDCEQAVLEIARLWKDSKTDPQIHSYLSKPTVVSGLVEILSASL 720

Query: 437  DDEILELVISILAELAARSEAIRLMILNSDPQLQVFLKLLKSSSLFLKASILLYLSKPQA 496
            + E+L   I IL+EL    E +   + + D        LLK+     +A++L+Y  +P  
Sbjct: 721  NREVLRRSIYILSELIFSDERVGETLNSVDSDFDCLAMLLKNG--LAEAALLIYQLRPVF 780

Query: 497  KQMISVEWLPLVLRVLEFGGSLQMLFSVRCAPYEAAFYLLDQLLKGFDEDRNLENGRHLI 556
             Q+   E +P +++V++        F +   P  AA  +L+Q+L G DE     N   +I
Sbjct: 781  AQLSEHELIPSLIQVIQNKSEDIDDFQLAIDPKAAAIAILEQILIGGDEYNRSVNASSVI 840

Query: 557  ALGGLSLLLRTLENGEIEERKNTVSMISCCIRADGTCRNYLAENLNKAALLELVVHESNK 616
            +  G+  +++ L+  + E R+  +S++ CC++A+ +C++ +A  +  + +LEL  H  N 
Sbjct: 841  SANGIPAIVKYLD--KTEGRRPVISILLCCMQAEKSCKSSIANRIELSPVLEL-FHAGND 900

Query: 617  NSDRCGLALLVELLCLSRRTRITKLLDELKERSNGSSIMNILSVDLHRARPEEQPLVATI 676
            +     +  L EL+ L+RRT   + L  +K+     S M+   V L  A  E Q  VA++
Sbjct: 901  SVRGICVEFLSELVRLNRRTSSNQTLQIIKD-EGAFSTMHTFLVYLQMAPMEHQIAVASL 960

Query: 677  LLQLDLMDDPLNCCSIFREEAIVTIITALNSQISREKTSENLARALLILGGRFSCTGEPC 736
            LLQLDL+ +P    SI+REEA+ T+I AL  Q            ALL L G  + +G+  
Sbjct: 961  LLQLDLLAEPRK-MSIYREEAVETLIEAL-WQKDFSNNQMKALDALLFLIGHVTSSGKSY 1020

Query: 737  PEIWLLQQAGFET--NSGDSSYSSRHVYDDLVQSYEEEED-LVNWQLKASTVFFNHGHKS 796
             E  LL+ AGF+   N    +    H  +D +++ E+E++ + +WQ + ++V  NH + S
Sbjct: 1021 TEAGLLKIAGFDQPYNVLMKAEQLGHSDNDFMETMEDEKNAMKSWQKRVASVLCNHENGS 1080

Query: 797  LLSALSTSMTNGIPRLAKASLVTVSWMSRYLFVIRDEKLCSMAPSILVPSLIKYLNYDKD 856
            +  AL   + +   ++AK+ LV  +W++  LF + D  +  +A   L+ +L+  L   K+
Sbjct: 1081 IFQALEECLKSNSLKMAKSCLVLATWLTHMLFTLPDTGVRDVARKSLLEALMNVLQSSKN 1140

Query: 857  VEDRVLASYTLLNLIKYTECKHIFGLFDEEARDHLENLSLVTWTAEELI 903
            +E+++LAS  L + I       +  ++ +     L  L   +  A +++
Sbjct: 1141 LEEKILASLALKSFISDPTVHEVLRVYAKSIYRILRKLKKYSTVAADIL 1181

BLAST of Cp4.1LG20g05970 vs. TrEMBL
Match: A0A0A0LYR5_CUCSA (Uncharacterized protein OS=Cucumis sativus GN=Csa_1G713660 PE=4 SV=1)

HSP 1 Score: 843.6 bits (2178), Expect = 2.3e-241
Identity = 456/582 (78.35%), Postives = 506/582 (86.94%), Query Frame = 1

Query: 347  EKIASVHDEHHETVKKSDYFRNLETNHLLSNELCKAISTICSSDILSECEIAIRVVSKAW 406
            + I   + E    + K+ + +    NHLLSN+  KAI+ ICSSDILSECEIA+RVV+KAW
Sbjct: 429  QNITKEYLESSNVITKNSFVKVEGRNHLLSNDFSKAITAICSSDILSECEIAVRVVTKAW 488

Query: 407  LDVDGDTTIEVALSTPPFVEGMLEVLLASNDDEILELVISILAELAARSEAIRLMILNSD 466
            LD  GD+ IEVALS PP VEGMLEVLLAS+DDEILELVIS+LAELAARSE I+ MILNSD
Sbjct: 489  LDAHGDSNIEVALSQPPVVEGMLEVLLASDDDEILELVISVLAELAARSEIIQQMILNSD 548

Query: 467  PQLQVFLKLLKSSSLFLKASILLYLSKPQAKQMISVEWLPLVLRVLEFGGSLQMLFSVRC 526
            PQLQVFLKLLKSSSLFLKASILLYLSKPQAKQMISVEWLPLVLRVLEFGG LQ LFSV+C
Sbjct: 549  PQLQVFLKLLKSSSLFLKASILLYLSKPQAKQMISVEWLPLVLRVLEFGGQLQTLFSVQC 608

Query: 527  APYEAAFYLLDQLLKGFDEDRNLENGRHLIALGGLSLLLRTLENGEIEERKNTVSMISCC 586
             P+EAAFYLLDQLLKGFDEDRNLEN RHLIALGGLSLLLR LE GEIEERKN+VS+ISCC
Sbjct: 609  KPHEAAFYLLDQLLKGFDEDRNLENSRHLIALGGLSLLLRRLERGEIEERKNSVSIISCC 668

Query: 587  IRADGTCRNYLAENLNKAALLELVVHESNKNSDRCGLALLVELLCLSRRTRITKLLDELK 646
            I+ADG+CRNYLAENLNKA+LLELVVHESNKNSDR GLALLV+LLCLSRRTRITKLLD LK
Sbjct: 669  IQADGSCRNYLAENLNKASLLELVVHESNKNSDRGGLALLVDLLCLSRRTRITKLLDGLK 728

Query: 647  ERSNGSSIMNILSVDLHRARPEEQPLVATILLQLDLMDDPLNCCSIFREEAIVTIITALN 706
            E  +G  IMNILSV L RA PEEQPLVAT LLQLD M+D LN CSIFREEAIVTIITALN
Sbjct: 729  EGWSGLGIMNILSVYLQRALPEEQPLVATTLLQLDFMEDALN-CSIFREEAIVTIITALN 788

Query: 707  SQISREKTSENLARALLILGGRFSCTGEPCPEIWLLQQAGFETNSGDSSYSSRHVYDDLV 766
            ++I  EK  +NLARALLILGGRFSCTGEP  E WLL+ AGF+ NSGDSS+ S+H+YDD+V
Sbjct: 789  ARICGEKAQDNLARALLILGGRFSCTGEPSTENWLLKLAGFKENSGDSSH-SKHLYDDVV 848

Query: 767  QSYEEEEDLVNWQLKASTVFFNHGHKSLLSALSTSMTNGI-PRLAKASLVTVSWMSRYLF 826
            Q YEEEE+++NWQLKA++V FNHGHKSLLS+LSTSMT+ I P LAKA L+T+SWMSRYLF
Sbjct: 849  QLYEEEEEVMNWQLKAASVLFNHGHKSLLSSLSTSMTSCIRPSLAKACLITLSWMSRYLF 908

Query: 827  VIRDEKLCSMAPSILVPSLIKYLNYDKDVEDRVLASYTLLNLIKYTECKHIFGLFDEEAR 886
            VIR+EKLC MAPSILVP LIKYLN+DK VED+VLASY+LLNL KYTECKHIF LFD+EA 
Sbjct: 909  VIREEKLCLMAPSILVPPLIKYLNHDKAVEDQVLASYSLLNLGKYTECKHIFRLFDDEAL 968

Query: 887  DHLENLSLVTWTAEELISIVKSGSMHRYDTEQKNSYTQRSTR 928
            DHL NLSLVTWTAEELI I+ SGSM  Y TE++NS  Q STR
Sbjct: 969  DHLRNLSLVTWTAEELILIITSGSMDPY-TERENSDNQGSTR 1007

BLAST of Cp4.1LG20g05970 vs. TrEMBL
Match: V4UCT6_9ROSI (Uncharacterized protein OS=Citrus clementina GN=CICLE_v10014170mg PE=4 SV=1)

HSP 1 Score: 775.8 bits (2002), Expect = 6.0e-221
Identity = 462/934 (49.46%), Postives = 616/934 (65.95%), Query Frame = 1

Query: 1   MASLKELLTQEGIEGSDFSRAQKHSSSKARSRAAPD-DSITLPIYICHDKKITDSSKKKV 60
           MASL++LL +EG E       +     K + R + D DSI LPIYICHD K  D SK++ 
Sbjct: 1   MASLQQLLAEEGFERGHRQFTEGPKPMKLKDRTSTDHDSIALPIYICHDLKSFDFSKQRS 60

Query: 61  EKSIMRDGFSLYSSNRLGSISETLLCKSM--EASAIDEISIRSVVSILSGYVGRYSKDDK 120
           +K++ R  +S+ SS R GS S++     +  E  AIDE+++R+V+SIL GY+GRY KD  
Sbjct: 61  DKAVSRQEYSIKSSEREGSNSKSSRIDGIGREEPAIDEVAVRAVISILGGYIGRYLKDGI 120

Query: 121 FREMVRKICSSCLLRNGGME--SGVCSNFEMGMKSVDRLIEERHGNERELRVKASRSSLG 180
           FRE VR+  +SCL R    E  +G+ +N E+G++S+D+L+E + G +RE R+K  R+S+ 
Sbjct: 121 FRESVREKFNSCLERRKRKEPDNGILANLELGVESIDKLVEGK-GADREHRMKLLRNSIQ 180

Query: 181 FFNTVISSLNSSKK--SKNCA--QSHLSACAHLYLAIVYKIEKNEKVSAKHVLQVFTDSP 240
             + +++SLNS K   S  C    SHLSA A LYL+I+YK++KN+++SA+H+LQVF DSP
Sbjct: 181 LLS-IVASLNSKKTRHSSTCGIPNSHLSAFAQLYLSIIYKLDKNDRISARHLLQVFCDSP 240

Query: 241 FFARTHLLPELWEHFFLPHLLHLKVWYNQELEFMSN------DRKIKALSKLYNDHMNRG 300
           + +RTHLLP+LWEHFFLPHLLHLKVWY++ELE +SN      D+++KAL K +ND M+ G
Sbjct: 241 YLSRTHLLPDLWEHFFLPHLLHLKVWYHKELELLSNLDYGEKDKRMKALGKAFNDRMDMG 300

Query: 301 TLKFALYYEQQLNDGAKAPHVPLVPLPSQ----SIKRASRKSLD--SSNNKNLYHAVFGP 360
           T +FALYY+  L  GA+ P VP VPLPS+    S++R S  S    SS +KNL+  VFGP
Sbjct: 301 TTQFALYYKNWLKIGAQLPAVPSVPLPSRTSYGSLRRRSSDSYTSYSSQSKNLFRTVFGP 360

Query: 361 ------NLDQQLAEKIA--SVHDEHH----------ETVKKSDYF--------------- 420
                 +LD      I   ++  EH              KKS+ F               
Sbjct: 361 TERRSMDLDHLNRASINAWNLQKEHKSKRNQKAELWSESKKSERFRLFACQSTPAEEFVV 420

Query: 421 ----------RNLETNHLLSNELCKAISTICSSDILSECEIAIRVVSKAWLDVDGDTTIE 480
                     RN + N   ++EL +A +TICSSD LSECE+AIRV+ K WL+  GD+ +E
Sbjct: 421 NGEHSRNNSRRNEDKNRNHTSELSRANTTICSSDDLSECELAIRVIVKTWLNSHGDSAVE 480

Query: 481 VALSTPPFVEGMLEVLLASNDDEILELVISILAELAARSEAIRLMILNSDPQLQVFLKLL 540
             LS  P +EGMLEVL ASN+DEILEL IS+LAEL A++E+ R ++LN DPQL++F+KLL
Sbjct: 481 AELSKAPIIEGMLEVLFASNEDEILELAISMLAELVAKNESNRQIVLNFDPQLEIFIKLL 540

Query: 541 KSSSLFLKASILLYLSKPQAKQMISVEWLPLVLRVLEFGGSLQMLFSVRCAPYEAAFYLL 600
           +S+SLFLKAS+LLYL KP+AKQMIS EW+PL+LRVLEFG  +Q LF+V C+   AAFY L
Sbjct: 541 RSTSLFLKASVLLYLLKPKAKQMISTEWVPLILRVLEFGDQIQTLFTVCCSAQVAAFYFL 600

Query: 601 DQLLKGFDEDRNLENGRHLIALGGLSLLLRTLENGEIEERKNTVSMISCCIRADGTCRNY 660
           +QL+ GFDED+N EN R +++ GGL+LL+  +E GEI ER+NT S+I CCI+AD  CR+Y
Sbjct: 601 EQLVNGFDEDKNFENARAVVSHGGLALLVGRIEKGEIHERQNTASIIICCIQADEKCRSY 660

Query: 661 LAENLNKAALLELVVHESNKNSDRCGLALLVELLCLSRRTRITKLLDELKERSNGSSIMN 720
           LAENLNKA+LLEL+V E N N +RC +ALL ELLCL+RRT++ K LD L     G S M+
Sbjct: 661 LAENLNKASLLELIVLE-NHNCNRCAIALLTELLCLARRTQMMKFLDRLNNGWGGLSTMH 720

Query: 721 ILSVDLHRARPEEQPLVATILLQLDLMDDPLNCCSIFREEAIVTIITALNSQISREKTSE 780
           I    L RA  EE+PLVA ILLQLDL+ DP + CS++REEA+  + +A+N Q   EK  E
Sbjct: 721 IFLAYLQRASSEERPLVAAILLQLDLLGDPSH-CSMYREEAVDALTSAMNCQTCSEKIQE 780

Query: 781 NLARALLILGGRF-SCTGEPCPEIWLLQQAGFETNSGDSSYSSRHVYDDLVQSYEEEEDL 840
             ARALL+LGG F S   E   E WLL+ AGF  +S DS Y      D+ +   EEE+  
Sbjct: 781 KSARALLMLGGLFPSYIEEATSEKWLLKLAGFNEHSDDSFYGK----DENLN--EEEKAT 840

Query: 841 VNWQLKASTVFFNHGHKSLLSALSTSMTNGIPRLAKASLVTVSWMSRYLFVIRDEKLCSM 870
             WQ KA+      G K  L+AL+  M NG P LA+ASL TV+WMSR+L    DE   + 
Sbjct: 841 EIWQQKAAMALLKSGSKGFLAALANCMVNGTPSLARASLFTVAWMSRFLHSAVDENFLTT 900

BLAST of Cp4.1LG20g05970 vs. TrEMBL
Match: V4W761_9ROSI (Uncharacterized protein OS=Citrus clementina GN=CICLE_v10014170mg PE=4 SV=1)

HSP 1 Score: 775.8 bits (2002), Expect = 6.0e-221
Identity = 462/934 (49.46%), Postives = 616/934 (65.95%), Query Frame = 1

Query: 1   MASLKELLTQEGIEGSDFSRAQKHSSSKARSRAAPD-DSITLPIYICHDKKITDSSKKKV 60
           MASL++LL +EG E       +     K + R + D DSI LPIYICHD K  D SK++ 
Sbjct: 1   MASLQQLLAEEGFERGHRQFTEGPKPMKLKDRTSTDHDSIALPIYICHDLKSFDFSKQRS 60

Query: 61  EKSIMRDGFSLYSSNRLGSISETLLCKSM--EASAIDEISIRSVVSILSGYVGRYSKDDK 120
           +K++ R  +S+ SS R GS S++     +  E  AIDE+++R+V+SIL GY+GRY KD  
Sbjct: 61  DKAVSRQEYSIKSSEREGSNSKSSRIDGIGREEPAIDEVAVRAVISILGGYIGRYLKDGI 120

Query: 121 FREMVRKICSSCLLRNGGME--SGVCSNFEMGMKSVDRLIEERHGNERELRVKASRSSLG 180
           FRE VR+  +SCL R    E  +G+ +N E+G++S+D+L+E + G +RE R+K  R+S+ 
Sbjct: 121 FRESVREKFNSCLERRKRKEPDNGILANLELGVESIDKLVEGK-GADREHRMKLLRNSIQ 180

Query: 181 FFNTVISSLNSSKK--SKNCA--QSHLSACAHLYLAIVYKIEKNEKVSAKHVLQVFTDSP 240
             + +++SLNS K   S  C    SHLSA A LYL+I+YK++KN+++SA+H+LQVF DSP
Sbjct: 181 LLS-IVASLNSKKTRHSSTCGIPNSHLSAFAQLYLSIIYKLDKNDRISARHLLQVFCDSP 240

Query: 241 FFARTHLLPELWEHFFLPHLLHLKVWYNQELEFMSN------DRKIKALSKLYNDHMNRG 300
           + +RTHLLP+LWEHFFLPHLLHLKVWY++ELE +SN      D+++KAL K +ND M+ G
Sbjct: 241 YLSRTHLLPDLWEHFFLPHLLHLKVWYHKELELLSNLDYGEKDKRMKALGKAFNDRMDMG 300

Query: 301 TLKFALYYEQQLNDGAKAPHVPLVPLPSQ----SIKRASRKSLD--SSNNKNLYHAVFGP 360
           T +FALYY+  L  GA+ P VP VPLPS+    S++R S  S    SS +KNL+  VFGP
Sbjct: 301 TTQFALYYKNWLKIGAQLPAVPSVPLPSRTSYGSLRRRSSDSYTSYSSQSKNLFRTVFGP 360

Query: 361 ------NLDQQLAEKIA--SVHDEHH----------ETVKKSDYF--------------- 420
                 +LD      I   ++  EH              KKS+ F               
Sbjct: 361 TERRSMDLDHLNRASINAWNLQKEHKSKRNQKAELWSESKKSERFRLFACQSTPAEEFVV 420

Query: 421 ----------RNLETNHLLSNELCKAISTICSSDILSECEIAIRVVSKAWLDVDGDTTIE 480
                     RN + N   ++EL +A +TICSSD LSECE+AIRV+ K WL+  GD+ +E
Sbjct: 421 NGEHSRNNSRRNEDKNRNHTSELSRANTTICSSDDLSECELAIRVIVKTWLNSHGDSAVE 480

Query: 481 VALSTPPFVEGMLEVLLASNDDEILELVISILAELAARSEAIRLMILNSDPQLQVFLKLL 540
             LS  P +EGMLEVL ASN+DEILEL IS+LAEL A++E+ R ++LN DPQL++F+KLL
Sbjct: 481 AELSKAPIIEGMLEVLFASNEDEILELAISMLAELVAKNESNRQIVLNFDPQLEIFIKLL 540

Query: 541 KSSSLFLKASILLYLSKPQAKQMISVEWLPLVLRVLEFGGSLQMLFSVRCAPYEAAFYLL 600
           +S+SLFLKAS+LLYL KP+AKQMIS EW+PL+LRVLEFG  +Q LF+V C+   AAFY L
Sbjct: 541 RSTSLFLKASVLLYLLKPKAKQMISTEWVPLILRVLEFGDQIQTLFTVCCSAQVAAFYFL 600

Query: 601 DQLLKGFDEDRNLENGRHLIALGGLSLLLRTLENGEIEERKNTVSMISCCIRADGTCRNY 660
           +QL+ GFDED+N EN R +++ GGL+LL+  +E GEI ER+NT S+I CCI+AD  CR+Y
Sbjct: 601 EQLVNGFDEDKNFENARAVVSHGGLALLVGRIEKGEIHERQNTASIIICCIQADEKCRSY 660

Query: 661 LAENLNKAALLELVVHESNKNSDRCGLALLVELLCLSRRTRITKLLDELKERSNGSSIMN 720
           LAENLNKA+LLEL+V E N N +RC +ALL ELLCL+RRT++ K LD L     G S M+
Sbjct: 661 LAENLNKASLLELIVLE-NHNCNRCAIALLTELLCLARRTQMMKFLDRLNNGWGGLSTMH 720

Query: 721 ILSVDLHRARPEEQPLVATILLQLDLMDDPLNCCSIFREEAIVTIITALNSQISREKTSE 780
           I    L RA  EE+PLVA ILLQLDL+ DP + CS++REEA+  + +A+N Q   EK  E
Sbjct: 721 IFLAYLQRASSEERPLVAAILLQLDLLGDPSH-CSMYREEAVDALTSAMNCQTCSEKIQE 780

Query: 781 NLARALLILGGRF-SCTGEPCPEIWLLQQAGFETNSGDSSYSSRHVYDDLVQSYEEEEDL 840
             ARALL+LGG F S   E   E WLL+ AGF  +S DS Y      D+ +   EEE+  
Sbjct: 781 KSARALLMLGGLFPSYIEEATSEKWLLKLAGFNEHSDDSFYGK----DENLN--EEEKAT 840

Query: 841 VNWQLKASTVFFNHGHKSLLSALSTSMTNGIPRLAKASLVTVSWMSRYLFVIRDEKLCSM 870
             WQ KA+      G K  L+AL+  M NG P LA+ASL TV+WMSR+L    DE   + 
Sbjct: 841 EIWQQKAAMALLKSGSKGFLAALANCMVNGTPSLARASLFTVAWMSRFLHSAVDENFLTT 900

BLAST of Cp4.1LG20g05970 vs. TrEMBL
Match: A0A0S3R2S5_PHAAN (Uncharacterized protein OS=Vigna angularis var. angularis GN=Vigan.01G260300 PE=4 SV=1)

HSP 1 Score: 754.6 bits (1947), Expect = 1.4e-214
Identity = 454/1026 (44.25%), Postives = 640/1026 (62.38%), Query Frame = 1

Query: 1    MASLKELLTQEGIEGSDFSRAQKHSSSKARSRAAPDDSI----TLPIYICHDKKITDSSK 60
            M SL+ELLT+EG     +      +  K + + +P        +LP++IC+D+K  D S 
Sbjct: 1    MTSLRELLTEEGF----YQTTSNLNPLKPKFKFSPQTPEIIINSLPLHICNDRKSLDCSN 60

Query: 61   KKVEKSIMRDGFSLYSSN--RLGSISE------TLLCKSMEASA--IDEISIRSVVSILS 120
              ++ S +R G   + SN  R+GS+SE      +LL          ++E++ R+VVSILS
Sbjct: 61   NNLDNSTIRTGSWSFQSNSQRVGSVSEIWKNSKSLLSPGSRTVGPPMNEVATRAVVSILS 120

Query: 121  GYVGRYSKDDKFREMVRKICSSCLLR--NG-GMESGVCSNFEMGMKSVDRLIEERHGNER 180
            GY+GR+ KDD FR++VR  CSS L+R  NG G E  V  N ++ M+++ +L++++ G  +
Sbjct: 121  GYIGRFLKDDHFRKIVRDKCSSYLIRRRNGSGSEDEVLVNMKLAMENIYKLVQDQ-GTRK 180

Query: 181  ELRVKASRSSLGFFNTVISSLNSSKKSKNCA-------QSHLSACAHLYLAIVYKIEKNE 240
            E+++++ R+S+    T++SSLNS  K+   A        SH+SACA LYLAIVYK++KN 
Sbjct: 181  EIKMESLRNSIELL-TIVSSLNSKSKTSKEAGSTCGIPNSHISACAQLYLAIVYKLQKNN 240

Query: 241  KVSAKHVLQVFTDSPFFARTHLLPELWEHFFLPHLLHLKVWYNQELEFMS--------ND 300
            ++ A+H+LQVF+DSPF ART+LLP+LWEH FLPHLLHLK+WY +EL+ +S         D
Sbjct: 241  RICARHLLQVFSDSPFLARTYLLPDLWEHVFLPHLLHLKIWYAEELDALSASSECQDAKD 300

Query: 301  RKIKALSKLYNDHMNRGTLKFALYYEQQLNDGAKAPHVPLVPLPSQSIKRASRKS----- 360
              ++ LSK+Y   ++ GT  FALYY+Q L  GA  P +P+VPLPS+  + +SRK      
Sbjct: 301  NTMRTLSKVYGKKVDTGTALFALYYKQWLKVGANEPPLPVVPLPSRPSRGSSRKMSSDSF 360

Query: 361  -LDSSNNKNLYHAVFGPNLDQQ----------LAEKIASVHDE-------HHETVKKS-- 420
             L SS NKNLY  VFGP L+ +          L  K  S +DE       +  +++K   
Sbjct: 361  VLSSSTNKNLYKEVFGPKLELKSTALADQNGLLTIKWGSGNDENLYGDDYNRSSLQKQDS 420

Query: 421  ------------------------DYF-----RNLETNHL------------------LS 480
                                    DYF     R+++   L                  LS
Sbjct: 421  IFLGKSSTSIDNSYAELKPGSQRLDYFQCFSCRSMQAESLTNSNYTSCNGSFRNEATALS 480

Query: 481  NELCKAISTICSSDILSECEIAIRVVSKAWLDVDGDTTIEVALSTPPFVEGMLEVLLASN 540
            +E  +AI+TICSSD+LSECE AIRV++KAWL+   D  IE A+S    VE +LE+L AS+
Sbjct: 481  SEFVRAITTICSSDVLSECEFAIRVITKAWLNSHVDPLIEEAVSQSSVVEAILEILFASS 540

Query: 541  DDEILELVISILAELAARSEAIRLMILNSDPQLQVFLKLLKSSSLFLKASILLYLSKPQA 600
             DEILEL IS+LAEL  R++AIR +IL+ DPQL +F++LL+ + LFLKA++LLYL KPQA
Sbjct: 541  IDEILELAISVLAELVGRNDAIRQIILSWDPQLDIFVRLLRRTGLFLKAAVLLYLLKPQA 600

Query: 601  KQMISVEWLPLVLRVLEFGGSLQMLFSVRCAPYEAAFYLLDQLLKGFDEDRNLENGRHLI 660
            KQM+S EW+ LVLRVLEFG  +  LF+V+C P EAA Y +DQLL GFDED+NLEN R ++
Sbjct: 601  KQMLSPEWVSLVLRVLEFGDKVLTLFTVQCCPQEAAIYFMDQLLNGFDEDKNLENARQVV 660

Query: 661  ALGGLSLLLRTLENGEIEERKNTVSMISCCIRADGTCRNYLAENLNKAALLELVVHESNK 720
            +LGGL+ L++ +E GEI +R     ++  CI A+G+CR++L++N+ K++LLEL++ +++K
Sbjct: 661  SLGGLTFLMKRIEEGEIHQRNKAALIVYSCIHAEGSCRSFLSDNIKKSSLLELIILDNSK 720

Query: 721  NSDRCGLALLVELLCLSRRTRITKLLDELKERSNGSSIMNILSVDLHRARPEEQPLVATI 780
            +   C  A+L ELL L R T+I   L  LK+   G + M+IL + L RA PE++PLVA +
Sbjct: 721  SCSGCAFAVLAELLYLERTTKILNFLRGLKDGWGGLNTMHILFIYLQRAPPEQRPLVAAV 780

Query: 781  LLQLDLMDDPLNCCSIFREEAIVTIITALNSQISREKTSENLARALLILGGRFSCTGEPC 840
            LL LD+M+DP    S +R EAI  I+ AL+ Q+  +   E  ARALL+L G F+ TGE  
Sbjct: 781  LLMLDIMEDPFK-ESFYRAEAIEAIMVALDCQVCNDIIQEQSARALLLLAGHFTYTGESL 840

Query: 841  PEIWLLQQAGFETNSGD-SSYSSRHVYDDLVQSYEEEEDLVNWQLKASTVFFNHGHKSLL 900
             E   L QAGF+    + SSY    V  D     EE+ +   W+ KA+ V F  G+++LL
Sbjct: 841  MERTFLHQAGFQEKCMEGSSYGKEIVVCDSAHKNEEDNEAETWRRKAACVLFQSGNQNLL 900

Query: 901  SALSTSMTNGIPRLAKASLVTVSWMSRYLFVIRDEKLCSMAPSILVPSLIKYLNYDKDVE 922
             ALS S  NG+P LA+ASLVT+SWMS YL ++ D K   MA SIL P L+K LNYDKDV 
Sbjct: 901  KALSDSSINGVPSLARASLVTISWMSSYLHLVDDRKFPPMAFSILTPLLLKSLNYDKDVG 960

BLAST of Cp4.1LG20g05970 vs. TrEMBL
Match: G7IJR0_MEDTR (E3 ubiquitin-protein ligase LIN-like protein, putative OS=Medicago truncatula GN=MTR_2g028940 PE=4 SV=1)

HSP 1 Score: 754.2 bits (1946), Expect = 1.9e-214
Identity = 458/957 (47.86%), Postives = 619/957 (64.68%), Query Frame = 1

Query: 41  LPIYICHDKKITDSSKKKVEKSIMRDGFSLYSSNRLGSISETLLCKSM--EASAIDEISI 100
           L  YIC+  K + SS     K             R+GS SE    KS+  E S +DE SI
Sbjct: 13  LSSYICNGPKGSSSSSSSQFK-------------RVGSTSERSNPKSLVSEDSRMDESSI 72

Query: 101 -RSVVSILSGYVGRYSKDDKFREMVRKICSSCLLRN---------GGMESGVCSNFEMGM 160
             +V+ ILSGY+GRY +DD FR ++R+ C+S L R          G  +  +  N E  M
Sbjct: 73  ITAVIGILSGYIGRYVRDDNFRRIIREKCNSFLDRVRTRKRRKDLGDDDDEIFVNIEFCM 132

Query: 161 KSVDRLIEERHGNERELRVKASRSSLGFFNTVISSLNSSKKSKNCAQSHLSACAHLYLAI 220
           K +D+LIE++   ++   +K+ R+S+    T ISSLNSSK       S+LS+CAHLYLAI
Sbjct: 133 KKIDKLIEDQGTKKQVTMMKSLRNSIELL-TKISSLNSSK-------SYLSSCAHLYLAI 192

Query: 221 VYKIEKNEKVSAKHVLQVFTDSPFFARTHLLPELWEHFFLPHLLHLKVWYNQELEFMSN- 280
            YK+ KN++VS+KH+LQVF  SP  ART+LLP+LW+H FLPHLLHLK+WY  E EF+SN 
Sbjct: 193 AYKLHKNDRVSSKHLLQVFCYSPNLARTYLLPDLWDHLFLPHLLHLKIWYTSEFEFLSNE 252

Query: 281 -----DRKIKALSKLYNDHMNRGTLKFALYYEQQLN-DGAKAPHVPLVPLPSQSIKRASR 340
                ++K+K L+K+YN+ M+ GT  FA+YY+Q L   GA    +P+VPLPS+   R+SR
Sbjct: 253 FHGEKEKKVKVLNKVYNEKMDSGTYLFAMYYKQWLKVSGASELPLPIVPLPSRPSFRSSR 312

Query: 341 K-SLDS-----SNNKNLYHAVFGPNLDQQLA------------------EKIASVHDEHH 400
           + S DS     S N NLY  VFG   +QQ +                  +K     D   
Sbjct: 313 RMSSDSSISNTSINPNLYKEVFGLKQEQQKSTNLRDQTGILTPRKGLEIDKKMYGDDYRC 372

Query: 401 ETVKKS---------------------DYFRNLE--------------------TNHLLS 460
            +V+K                      DYF+ L                     ++ +LS
Sbjct: 373 SSVQKEDRKSFGRSSNQIDKNKAHQRLDYFQCLSCRFIPTETMSKINYIKSKNASSSVLS 432

Query: 461 NELCKAISTICSSDILSECEIAIRVVSKAWLDVDGDTTIEVALSTPPFVEGMLEVLLASN 520
           ++L +AI+TICSSDIL+ECE AIRVV+KAWL+  GD  IE AL+    V+G+LEVL  S 
Sbjct: 433 SDLVEAITTICSSDILTECEFAIRVVTKAWLNSPGDPLIEEALTQSIVVQGILEVLFVST 492

Query: 521 DDEILELVISILAELAARSEAIRLMILNSDPQLQVFLKLLKSSSLFLKASILLYLSKPQA 580
           +DEILEL+ISILAEL  R+++IR +ILNSDPQL++F++LL+S+SLFLKAS+LLYLSKP A
Sbjct: 493 EDEILELIISILAELITRNDSIRQIILNSDPQLELFVRLLRSTSLFLKASVLLYLSKPMA 552

Query: 581 KQMISVEWLPLVLRVLEFGGSLQMLFSVRCAPYEAAFYLLDQLLKGFDEDRNLENGRHLI 640
           KQMIS EW+PL+LRVLEFG  LQ LF+V+C+P  AAFY+LDQLL GFDED+NLEN R ++
Sbjct: 553 KQMISSEWVPLILRVLEFGDKLQTLFTVQCSPQVAAFYILDQLLNGFDEDKNLENARQVL 612

Query: 641 ALGGLSLLLRTLENGEIEERKNTVSMISCCIRADGTCRNYLAENLNKAALLELVVHESNK 700
           +LGGL+LL++ +++GEI E++N+  +ISCC+RA+G+CR+YLAEN+NK++LLEL+V    +
Sbjct: 613 SLGGLTLLIKRIDDGEIHEKENSALIISCCLRAEGSCRSYLAENINKSSLLELIVLGRKQ 672

Query: 701 NSDRCGLALLVELLCLSRRTRITKLLDELKERSNGSSIMNILSVDLHRARPEEQPLVATI 760
           NS    L+LL ELL L RRT+I K L  LK+  +G + M+I  + L +A  EE+PLVA I
Sbjct: 673 NSSGYALSLLFELLHLDRRTKILKFLRGLKDGWSGLNTMHIFFIYLQKAPLEERPLVAVI 732

Query: 761 LLQLDLMDDPLNCCSIFREEAIVTIITALNSQISREKTSENLARALLILGGRFSCTGEPC 820
           LL LDLM+D     SI+REEAI  I+ ALN ++  ++  +  A+ALL+LGG FS  GE  
Sbjct: 733 LLMLDLMEDKHLKGSIYREEAIEAIVAALNCKMCDDRVQQQSAKALLLLGGHFSYAGESL 792

Query: 821 PEIWLLQQAGFETNSGDSSY---SSRHVYDDLVQSYEEEEDLVNWQLKASTVFFNHGHKS 880
            E  LLQ+AGF+    + S+       +YD +   ++EEE+  +WQ +A+ V F  G+K 
Sbjct: 793 MENLLLQKAGFQEFCLEDSFPPCKEIVLYDSI---HKEEEEGESWQKRAACVLFKSGNKR 852

Query: 881 LLSALSTSMTNGIPRLAKASLVTVSWMSRYLFVIRDEKLCSMAPSILVPSLIKYLNYDKD 911
           LLSAL+ S+ NGIP LA+ASL T+SWM  YL ++ D KL  MA SIL P L++ LNYD D
Sbjct: 853 LLSALANSIANGIPSLARASLTTISWMCSYLHLVEDTKLPQMAFSILTPQLLQSLNYDND 912

BLAST of Cp4.1LG20g05970 vs. NCBI nr
Match: gi|659118365|ref|XP_008459082.1| (PREDICTED: LOW QUALITY PROTEIN: putative E3 ubiquitin-protein ligase LIN-2 [Cucumis melo])

HSP 1 Score: 856.7 bits (2212), Expect = 3.8e-245
Identity = 463/582 (79.55%), Postives = 508/582 (87.29%), Query Frame = 1

Query: 347  EKIASVHDEHHETVKKSDYFRNLETNHLLSNELCKAISTICSSDILSECEIAIRVVSKAW 406
            + I   + E    + K+   R    NHLLSN+L KAI+ ICSSDILSECEIAIRVV+KAW
Sbjct: 429  QSITKEYLESSNVITKNSSVRVEGRNHLLSNDLSKAITAICSSDILSECEIAIRVVTKAW 488

Query: 407  LDVDGDTTIEVALSTPPFVEGMLEVLLASNDDEILELVISILAELAARSEAIRLMILNSD 466
            LD  GDTTIEVALS PP VEGMLEVLLAS+DDEILELVIS LAELA RSE IR MILNSD
Sbjct: 489  LDAHGDTTIEVALSKPPVVEGMLEVLLASDDDEILELVISFLAELAVRSEVIRQMILNSD 548

Query: 467  PQLQVFLKLLKSSSLFLKASILLYLSKPQAKQMISVEWLPLVLRVLEFGGSLQMLFSVRC 526
            PQLQVFLKLLKSSSLFLKASILLYLSKPQAKQMISVEWLPLVLRVLEFGG LQ LFSVRC
Sbjct: 549  PQLQVFLKLLKSSSLFLKASILLYLSKPQAKQMISVEWLPLVLRVLEFGGQLQTLFSVRC 608

Query: 527  APYEAAFYLLDQLLKGFDEDRNLENGRHLIALGGLSLLLRTLENGEIEERKNTVSMISCC 586
             P+EAAFYLLDQLLKGFDEDRNLEN RHLI+LGGLSLLLR LE GEIEERKN+VS+ISCC
Sbjct: 609  KPHEAAFYLLDQLLKGFDEDRNLENSRHLISLGGLSLLLRRLERGEIEERKNSVSIISCC 668

Query: 587  IRADGTCRNYLAENLNKAALLELVVHESNKNSDRCGLALLVELLCLSRRTRITKLLDELK 646
            ++ADG+CRNYLAENLNKA+LLEL+VHESNKNS R GLALLV+LLCLSRRTRITKLLD LK
Sbjct: 669  VQADGSCRNYLAENLNKASLLELIVHESNKNSGRGGLALLVDLLCLSRRTRITKLLDGLK 728

Query: 647  ERSNGSSIMNILSVDLHRARPEEQPLVATILLQLDLMDDPLNCCSIFREEAIVTIITALN 706
            E  +G  IMNILSV L RA PEEQPLVAT+LLQLD M+D LN CSIFREEAIVTIITALN
Sbjct: 729  EGWSGLGIMNILSVYLQRALPEEQPLVATMLLQLDFMEDALN-CSIFREEAIVTIITALN 788

Query: 707  SQISREKTSENLARALLILGGRFSCTGEPCPEIWLLQQAGFETNSGDSSYSSRHVYDDLV 766
            ++IS EK  +NLARALLILGGRFSCTGEP  E WLL+ AGF  NSGDSS+ S+H+YDD+V
Sbjct: 789  ARISGEKVQDNLARALLILGGRFSCTGEPSTENWLLKLAGFRENSGDSSH-SKHLYDDVV 848

Query: 767  QSYEEEEDLVNWQLKASTVFFNHGHKSLLSALSTSMTNGI-PRLAKASLVTVSWMSRYLF 826
              YEEEE++VNWQLKA+TV FNHGHKSLLS+LSTSMT+ + P LAKA L+T+SWMSRYLF
Sbjct: 849  LLYEEEEEVVNWQLKAATVLFNHGHKSLLSSLSTSMTSCVRPSLAKACLITLSWMSRYLF 908

Query: 827  VIRDEKLCSMAPSILVPSLIKYLNYDKDVEDRVLASYTLLNLIKYTECKHIFGLFDEEAR 886
            VIRDEKLC MAPSILVP+LIKYLN+DK VED+VLASY+LLNL KYTECKHIF LFDEEA 
Sbjct: 909  VIRDEKLCLMAPSILVPALIKYLNHDKAVEDQVLASYSLLNLGKYTECKHIFRLFDEEAL 968

Query: 887  DHLENLSLVTWTAEELISIVKSGSMHRYDTEQKNSYTQRSTR 928
            DHL NLSLVTWTAEELI I+ SGSMH + TEQ+NS+TQ STR
Sbjct: 969  DHLRNLSLVTWTAEELILIITSGSMHPH-TEQENSHTQGSTR 1007

BLAST of Cp4.1LG20g05970 vs. NCBI nr
Match: gi|778664640|ref|XP_004145484.2| (PREDICTED: putative E3 ubiquitin-protein ligase LIN-2 [Cucumis sativus])

HSP 1 Score: 843.6 bits (2178), Expect = 3.3e-241
Identity = 456/582 (78.35%), Postives = 506/582 (86.94%), Query Frame = 1

Query: 347  EKIASVHDEHHETVKKSDYFRNLETNHLLSNELCKAISTICSSDILSECEIAIRVVSKAW 406
            + I   + E    + K+ + +    NHLLSN+  KAI+ ICSSDILSECEIA+RVV+KAW
Sbjct: 429  QNITKEYLESSNVITKNSFVKVEGRNHLLSNDFSKAITAICSSDILSECEIAVRVVTKAW 488

Query: 407  LDVDGDTTIEVALSTPPFVEGMLEVLLASNDDEILELVISILAELAARSEAIRLMILNSD 466
            LD  GD+ IEVALS PP VEGMLEVLLAS+DDEILELVIS+LAELAARSE I+ MILNSD
Sbjct: 489  LDAHGDSNIEVALSQPPVVEGMLEVLLASDDDEILELVISVLAELAARSEIIQQMILNSD 548

Query: 467  PQLQVFLKLLKSSSLFLKASILLYLSKPQAKQMISVEWLPLVLRVLEFGGSLQMLFSVRC 526
            PQLQVFLKLLKSSSLFLKASILLYLSKPQAKQMISVEWLPLVLRVLEFGG LQ LFSV+C
Sbjct: 549  PQLQVFLKLLKSSSLFLKASILLYLSKPQAKQMISVEWLPLVLRVLEFGGQLQTLFSVQC 608

Query: 527  APYEAAFYLLDQLLKGFDEDRNLENGRHLIALGGLSLLLRTLENGEIEERKNTVSMISCC 586
             P+EAAFYLLDQLLKGFDEDRNLEN RHLIALGGLSLLLR LE GEIEERKN+VS+ISCC
Sbjct: 609  KPHEAAFYLLDQLLKGFDEDRNLENSRHLIALGGLSLLLRRLERGEIEERKNSVSIISCC 668

Query: 587  IRADGTCRNYLAENLNKAALLELVVHESNKNSDRCGLALLVELLCLSRRTRITKLLDELK 646
            I+ADG+CRNYLAENLNKA+LLELVVHESNKNSDR GLALLV+LLCLSRRTRITKLLD LK
Sbjct: 669  IQADGSCRNYLAENLNKASLLELVVHESNKNSDRGGLALLVDLLCLSRRTRITKLLDGLK 728

Query: 647  ERSNGSSIMNILSVDLHRARPEEQPLVATILLQLDLMDDPLNCCSIFREEAIVTIITALN 706
            E  +G  IMNILSV L RA PEEQPLVAT LLQLD M+D LN CSIFREEAIVTIITALN
Sbjct: 729  EGWSGLGIMNILSVYLQRALPEEQPLVATTLLQLDFMEDALN-CSIFREEAIVTIITALN 788

Query: 707  SQISREKTSENLARALLILGGRFSCTGEPCPEIWLLQQAGFETNSGDSSYSSRHVYDDLV 766
            ++I  EK  +NLARALLILGGRFSCTGEP  E WLL+ AGF+ NSGDSS+ S+H+YDD+V
Sbjct: 789  ARICGEKAQDNLARALLILGGRFSCTGEPSTENWLLKLAGFKENSGDSSH-SKHLYDDVV 848

Query: 767  QSYEEEEDLVNWQLKASTVFFNHGHKSLLSALSTSMTNGI-PRLAKASLVTVSWMSRYLF 826
            Q YEEEE+++NWQLKA++V FNHGHKSLLS+LSTSMT+ I P LAKA L+T+SWMSRYLF
Sbjct: 849  QLYEEEEEVMNWQLKAASVLFNHGHKSLLSSLSTSMTSCIRPSLAKACLITLSWMSRYLF 908

Query: 827  VIRDEKLCSMAPSILVPSLIKYLNYDKDVEDRVLASYTLLNLIKYTECKHIFGLFDEEAR 886
            VIR+EKLC MAPSILVP LIKYLN+DK VED+VLASY+LLNL KYTECKHIF LFD+EA 
Sbjct: 909  VIREEKLCLMAPSILVPPLIKYLNHDKAVEDQVLASYSLLNLGKYTECKHIFRLFDDEAL 968

Query: 887  DHLENLSLVTWTAEELISIVKSGSMHRYDTEQKNSYTQRSTR 928
            DHL NLSLVTWTAEELI I+ SGSM  Y TE++NS  Q STR
Sbjct: 969  DHLRNLSLVTWTAEELILIITSGSMDPY-TERENSDNQGSTR 1007

BLAST of Cp4.1LG20g05970 vs. NCBI nr
Match: gi|720052148|ref|XP_010272313.1| (PREDICTED: putative E3 ubiquitin-protein ligase LIN [Nelumbo nucifera])

HSP 1 Score: 798.9 bits (2062), Expect = 9.5e-228
Identity = 479/999 (47.95%), Postives = 642/999 (64.26%), Query Frame = 1

Query: 1   MASLKELLTQEGIEGSDFSRAQKHSSSKARSRAAPDDSITLPIYICHDKKITDSSKKKVE 60
           MASL+ELL +EG +G    + +K    K+R R   DDSI LP YICHD+K  DSSK+  +
Sbjct: 1   MASLQELLAEEGFQGGKTPKNRK--PVKSRERITSDDSIALPTYICHDRKNFDSSKQTPQ 60

Query: 61  KSIMRDGFSLYSSNRLGSISETLLCKSM---EA-------SAIDEISIRSVVSILSGYVG 120
           KS++R+  S+ SS R+ S S+    KS    EA       + +DE+++R+VVSIL GY+G
Sbjct: 61  KSLVRNASSVSSSKRVDSNSKRSNSKSSCMAEALPPQSGETIVDEVAVRAVVSILGGYIG 120

Query: 121 RYSKDDKFREMVRKICSSCLL-RNGGMESGVCSNFEMGMKSVDRLIEERHGNERELRVKA 180
           R+ KD+ FRE VR+ C SC+  R+   ++ + +N E+G++S++RL E  HG ++EL++K+
Sbjct: 121 RFIKDESFRERVREKCYSCMEGRSKDGDNAILANMELGIESIERLAEN-HGTKKELKMKS 180

Query: 181 SRSSLGFFNTVISSLNS--SKKSKNCA--QSHLSACAHLYLAIVYKIEKNEKVSAKHVLQ 240
            R+S+   + +++SLNS  SK    C    SHLSACA LYL+IVYK+EKN+++SA+H+LQ
Sbjct: 181 LRNSIRLLS-IVASLNSHNSKNDTTCGIPNSHLSACAQLYLSIVYKLEKNDRISARHLLQ 240

Query: 241 VFTDSPFFARTHLLPELWEHFFLPHLLHLKVWYNQELEFMSN------DRKIKALSKLYN 300
           VF DSPF ARTHLLP+LWEHFFLPH LHLK+WY++E E + N      +RK+KAL K+YN
Sbjct: 241 VFCDSPFLARTHLLPDLWEHFFLPHFLHLKIWYSKEEELILNWESGEKERKMKALIKVYN 300

Query: 301 DHMNRGTLKFALYYEQQLNDGAKAPHVPLVPLPSQ-----SIKRASRKSLDSSNNKNLYH 360
           + M+ GT +FALYY++ L  GAKAP +P V LPS+     + +R +     +S NK+LY 
Sbjct: 301 EKMDMGTSQFALYYKEWLKVGAKAPPMPSVSLPSRPSYGAAKRRGTSLGSQASLNKSLYR 360

Query: 361 AVFGPNLDQQ----------LAEKIASVHDEH--HET----------------------- 420
           AVFG   ++Q          L E++   +DEH  H T                       
Sbjct: 361 AVFGRINERQSLELENDTWSLEEEVKVCNDEHNIHRTRSVHSSGKGVHRRSISQQSRNPK 420

Query: 421 ------VKKSDYFRNLETNHLLSNELCKAISTICSSDILSECE---------IAIRVVS- 480
                  +KSDYFR        +  L +      +  I  E            AI+ +  
Sbjct: 421 AELWPETRKSDYFRFFPCRSEPAKNLVQGAHVSKNDSIRKESPSYLPSNSFGAAIKTICT 480

Query: 481 ---------------KAWLDVDGDTTIEVALSTPPFVEGMLEVLLASNDDEILELVISIL 540
                          KAWLD   D  IE  LS  P +EGMLEVL  S D+E LEL ISIL
Sbjct: 481 SQSLSDCELAIRVVAKAWLDSHADPVIETMLSKAPVLEGMLEVLFTSEDEETLELAISIL 540

Query: 541 AELAARSEAIRLMILNSDPQLQVFLKLLKSSSLFLKASILLYLSKPQAKQMISVEWLPLV 600
           AEL +R E  R +ILNSDPQL+V ++LL+S+SLFLKAS+LLYL KP+AKQM+S+EW+PLV
Sbjct: 541 AELVSRGEVNRQIILNSDPQLEVLMRLLRSNSLFLKASVLLYLLKPKAKQMLSIEWIPLV 600

Query: 601 LRVLEFGGSLQMLFSVRCAPYEAAFYLLDQLLKGFDEDRNLENGRHLIALGGLSLLLRTL 660
           LRV+EFG  LQ LFSV+C+P  AAFYLLDQLL GF+ED NLEN R ++ALGGLSLL+R+L
Sbjct: 601 LRVVEFGDELQTLFSVQCSPQVAAFYLLDQLLTGFNEDGNLENARQVVALGGLSLLVRSL 660

Query: 661 ENGEIEERKNTVSMISCCIRADGTCRNYLAENLNKAALLELVVHESNKNSDRCGLALLVE 720
           E G+ + R +  S+I+ CI+ADG+CRNYLA N+NKA++L+L++  +   S    L+LL+E
Sbjct: 661 ETGDPQSR-SAASIITSCIQADGSCRNYLANNINKASILQLLILGNRSRSSGSILSLLIE 720

Query: 721 LLCLSRRTRITKLLDELKERSNGSSIMNILSVDLHRARPEEQPLVATILLQLDLMDDPLN 780
           LLCL+RRT IT  L+ LK      + M+IL V L  A PE++ LVA ILLQLDL++D   
Sbjct: 721 LLCLNRRTEITSFLNGLKNNEERLNTMHILLVYLQWAPPEQRSLVAAILLQLDLLEDSFQ 780

Query: 781 CCSIFREEAIVTIITALNSQISREKTSENLARALLILGGRFSCTGEPCPEIWLLQQAGFE 840
             S++REEA+  IITAL+ +   EK  +  AR+LL+LGGRFS  GE   E WLL+QAGF+
Sbjct: 781 -YSVYREEAVDAIITALDCKTCNEKVQQQSARSLLLLGGRFSYMGEASTETWLLKQAGFD 840

Query: 841 TNSGDSSYSSRHVYDDLVQSYEEEEDLVNWQLKASTVFFNHGHKSLLSALSTSMTNGIPR 900
            ++ DS +    VYD+++   EEEE + NW  KA+TV    G    L ALS  M NGIP 
Sbjct: 841 DSTDDSFHGKEIVYDEILIQNEEEESIQNWLRKAATVLLTSGKNRFLVALSECMANGIPY 900

Query: 901 LAKASLVTVSWMSRYLFVIRDEKLCSMAPSILVPSLIKYLNYDKDVEDRVLASYTLLNLI 908
           LA+ASL+TV+W+SR L  I+D  L S A SILVP LI+ LNYD+ +E+RVLAS +LL LI
Sbjct: 901 LARASLITVAWLSRSLTSIQDSSLQSTACSILVPRLIETLNYDRALEERVLASLSLLCLI 960

BLAST of Cp4.1LG20g05970 vs. NCBI nr
Match: gi|568828212|ref|XP_006468438.1| (PREDICTED: putative E3 ubiquitin-protein ligase LIN isoform X4 [Citrus sinensis])

HSP 1 Score: 779.2 bits (2011), Expect = 7.8e-222
Identity = 464/934 (49.68%), Postives = 619/934 (66.27%), Query Frame = 1

Query: 1   MASLKELLTQEGIEGSDFSRAQKHSSSKARSRAAPD-DSITLPIYICHDKKITDSSKKKV 60
           MASL++LL +EG E       +     K + R + D DSI LPIYICHD K  D SK+  
Sbjct: 1   MASLQQLLAEEGFERGHRQFTEGPKPMKLKDRTSTDHDSIALPIYICHDLKSFDFSKQGS 60

Query: 61  EKSIMRDGFSLYSSNRLGSISETLLCKSM--EASAIDEISIRSVVSILSGYVGRYSKDDK 120
           +K++ R  +S+ SS R GS S++     +  E  AIDE+++R+V+SIL GY+GRY KD+ 
Sbjct: 61  DKAVSRQEYSIKSSEREGSNSKSSRIDGIGREEPAIDEVAVRAVISILGGYIGRYLKDEI 120

Query: 121 FREMVRKICSSCLLRNGGME--SGVCSNFEMGMKSVDRLIEERHGNERELRVKASRSSLG 180
           FRE VR+  +SCL R    E  +G+ +N E+G++S+D+L+E + G +RE R+K  R+S+ 
Sbjct: 121 FRESVREKFNSCLERRKRKEPDNGILANLELGVESIDKLVEGK-GADREHRMKLLRNSIQ 180

Query: 181 FFNTVISSLNSSKK--SKNCA--QSHLSACAHLYLAIVYKIEKNEKVSAKHVLQVFTDSP 240
             + +++SLNS K   S  C    SHLSA A LYL+I+YK++KN+++SA+H+LQVF DSP
Sbjct: 181 LLS-IVASLNSKKTRHSSTCGIPNSHLSAFAQLYLSIIYKLDKNDRISARHLLQVFCDSP 240

Query: 241 FFARTHLLPELWEHFFLPHLLHLKVWYNQELEFMSN------DRKIKALSKLYNDHMNRG 300
           + +RTHLLP+LWEHFFLPHLLHLKVWY++ELE +SN      D+++KAL K +ND M+ G
Sbjct: 241 YLSRTHLLPDLWEHFFLPHLLHLKVWYHKELELLSNLDYGEKDKRMKALGKAFNDRMDMG 300

Query: 301 TLKFALYYEQQLNDGAKAPHVPLVPLPSQ-SIKRASRKSLD-----SSNNKNLYHAVFGP 360
           T +FALYY+  L  GA+ P VP VPLPS+ S   + R+S D     SS NKNL+  VFGP
Sbjct: 301 TTQFALYYKNWLKIGAQLPAVPSVPLPSRTSYGSSRRRSSDSYTSYSSQNKNLFRTVFGP 360

Query: 361 ---------NLDQ------QLAEKIASVHDEHHE---TVKKSDYF--------------- 420
                    NL++       L ++  S  ++  E     KKS+ F               
Sbjct: 361 TERRSMDLDNLNRASINAWNLQKEHKSKRNQKAELWSESKKSERFRLFACQSTPAEEFVV 420

Query: 421 ----------RNLETNHLLSNELCKAISTICSSDILSECEIAIRVVSKAWLDVDGDTTIE 480
                     RN + N   ++EL +A +TICSSD LSECE+AIRV+ K WL+  GD+ +E
Sbjct: 421 NGEHSRNNSRRNEDKNRNHTSELSRANTTICSSDDLSECELAIRVIVKTWLNSHGDSAVE 480

Query: 481 VALSTPPFVEGMLEVLLASNDDEILELVISILAELAARSEAIRLMILNSDPQLQVFLKLL 540
             LS  P +EGMLEVL ASN+DEILEL IS+LAEL A++E+ R ++LN DPQL++F+KLL
Sbjct: 481 AELSKAPIIEGMLEVLFASNEDEILELAISMLAELVAKNESNRQIVLNFDPQLEIFIKLL 540

Query: 541 KSSSLFLKASILLYLSKPQAKQMISVEWLPLVLRVLEFGGSLQMLFSVRCAPYEAAFYLL 600
           +S+SLFLKAS+LLYL KP+AKQMIS EW+PL+LRVLEFG  +Q LF+V C+   AAFY L
Sbjct: 541 RSTSLFLKASVLLYLLKPKAKQMISTEWVPLILRVLEFGDQIQTLFTVCCSAQVAAFYFL 600

Query: 601 DQLLKGFDEDRNLENGRHLIALGGLSLLLRTLENGEIEERKNTVSMISCCIRADGTCRNY 660
           +QL+ GFDED+N EN R +++ GGL+LL+  +E GEI ER+NT S+I CCI+AD  CR+Y
Sbjct: 601 EQLVNGFDEDKNFENARAVVSHGGLALLVGRIEKGEIHERQNTASIIICCIQADEKCRSY 660

Query: 661 LAENLNKAALLELVVHESNKNSDRCGLALLVELLCLSRRTRITKLLDELKERSNGSSIMN 720
           LAENLNKA+LLEL+V E N N +RC +ALL ELLCL+RRT++ K LD L     G S M+
Sbjct: 661 LAENLNKASLLELIVLE-NHNCNRCAIALLTELLCLARRTQMMKFLDRLNNGWGGLSTMH 720

Query: 721 ILSVDLHRARPEEQPLVATILLQLDLMDDPLNCCSIFREEAIVTIITALNSQISREKTSE 780
           I    L RA  EE+PLVA ILLQLDL+ DP N CS++REEA+  + +A+N Q   EK  E
Sbjct: 721 IFLAYLQRASSEERPLVAAILLQLDLLGDPSN-CSMYREEAVDALTSAMNCQTCSEKIQE 780

Query: 781 NLARALLILGGRF-SCTGEPCPEIWLLQQAGFETNSGDSSYSSRHVYDDLVQSYEEEEDL 840
             ARALL+LGG F S   E   E WLL+ AGF  +S DS Y      D+ +   EEE+  
Sbjct: 781 KSARALLMLGGLFPSYIEEATSEKWLLKLAGFNEHSDDSFYGK----DENLN--EEEKAT 840

Query: 841 VNWQLKASTVFFNHGHKSLLSALSTSMTNGIPRLAKASLVTVSWMSRYLFVIRDEKLCSM 870
             WQ KA+      G K  L+AL+  M NG P LA+ASL TV+WMSR+L    DE   + 
Sbjct: 841 EIWQQKAAMALLKSGSKGFLAALANCMVNGTPSLARASLFTVAWMSRFLHSAVDENFLTT 900

BLAST of Cp4.1LG20g05970 vs. NCBI nr
Match: gi|567912861|ref|XP_006448744.1| (hypothetical protein CICLE_v10014170mg [Citrus clementina])

HSP 1 Score: 775.8 bits (2002), Expect = 8.6e-221
Identity = 462/934 (49.46%), Postives = 616/934 (65.95%), Query Frame = 1

Query: 1   MASLKELLTQEGIEGSDFSRAQKHSSSKARSRAAPD-DSITLPIYICHDKKITDSSKKKV 60
           MASL++LL +EG E       +     K + R + D DSI LPIYICHD K  D SK++ 
Sbjct: 1   MASLQQLLAEEGFERGHRQFTEGPKPMKLKDRTSTDHDSIALPIYICHDLKSFDFSKQRS 60

Query: 61  EKSIMRDGFSLYSSNRLGSISETLLCKSM--EASAIDEISIRSVVSILSGYVGRYSKDDK 120
           +K++ R  +S+ SS R GS S++     +  E  AIDE+++R+V+SIL GY+GRY KD  
Sbjct: 61  DKAVSRQEYSIKSSEREGSNSKSSRIDGIGREEPAIDEVAVRAVISILGGYIGRYLKDGI 120

Query: 121 FREMVRKICSSCLLRNGGME--SGVCSNFEMGMKSVDRLIEERHGNERELRVKASRSSLG 180
           FRE VR+  +SCL R    E  +G+ +N E+G++S+D+L+E + G +RE R+K  R+S+ 
Sbjct: 121 FRESVREKFNSCLERRKRKEPDNGILANLELGVESIDKLVEGK-GADREHRMKLLRNSIQ 180

Query: 181 FFNTVISSLNSSKK--SKNCA--QSHLSACAHLYLAIVYKIEKNEKVSAKHVLQVFTDSP 240
             + +++SLNS K   S  C    SHLSA A LYL+I+YK++KN+++SA+H+LQVF DSP
Sbjct: 181 LLS-IVASLNSKKTRHSSTCGIPNSHLSAFAQLYLSIIYKLDKNDRISARHLLQVFCDSP 240

Query: 241 FFARTHLLPELWEHFFLPHLLHLKVWYNQELEFMSN------DRKIKALSKLYNDHMNRG 300
           + +RTHLLP+LWEHFFLPHLLHLKVWY++ELE +SN      D+++KAL K +ND M+ G
Sbjct: 241 YLSRTHLLPDLWEHFFLPHLLHLKVWYHKELELLSNLDYGEKDKRMKALGKAFNDRMDMG 300

Query: 301 TLKFALYYEQQLNDGAKAPHVPLVPLPSQ----SIKRASRKSLD--SSNNKNLYHAVFGP 360
           T +FALYY+  L  GA+ P VP VPLPS+    S++R S  S    SS +KNL+  VFGP
Sbjct: 301 TTQFALYYKNWLKIGAQLPAVPSVPLPSRTSYGSLRRRSSDSYTSYSSQSKNLFRTVFGP 360

Query: 361 ------NLDQQLAEKIA--SVHDEHH----------ETVKKSDYF--------------- 420
                 +LD      I   ++  EH              KKS+ F               
Sbjct: 361 TERRSMDLDHLNRASINAWNLQKEHKSKRNQKAELWSESKKSERFRLFACQSTPAEEFVV 420

Query: 421 ----------RNLETNHLLSNELCKAISTICSSDILSECEIAIRVVSKAWLDVDGDTTIE 480
                     RN + N   ++EL +A +TICSSD LSECE+AIRV+ K WL+  GD+ +E
Sbjct: 421 NGEHSRNNSRRNEDKNRNHTSELSRANTTICSSDDLSECELAIRVIVKTWLNSHGDSAVE 480

Query: 481 VALSTPPFVEGMLEVLLASNDDEILELVISILAELAARSEAIRLMILNSDPQLQVFLKLL 540
             LS  P +EGMLEVL ASN+DEILEL IS+LAEL A++E+ R ++LN DPQL++F+KLL
Sbjct: 481 AELSKAPIIEGMLEVLFASNEDEILELAISMLAELVAKNESNRQIVLNFDPQLEIFIKLL 540

Query: 541 KSSSLFLKASILLYLSKPQAKQMISVEWLPLVLRVLEFGGSLQMLFSVRCAPYEAAFYLL 600
           +S+SLFLKAS+LLYL KP+AKQMIS EW+PL+LRVLEFG  +Q LF+V C+   AAFY L
Sbjct: 541 RSTSLFLKASVLLYLLKPKAKQMISTEWVPLILRVLEFGDQIQTLFTVCCSAQVAAFYFL 600

Query: 601 DQLLKGFDEDRNLENGRHLIALGGLSLLLRTLENGEIEERKNTVSMISCCIRADGTCRNY 660
           +QL+ GFDED+N EN R +++ GGL+LL+  +E GEI ER+NT S+I CCI+AD  CR+Y
Sbjct: 601 EQLVNGFDEDKNFENARAVVSHGGLALLVGRIEKGEIHERQNTASIIICCIQADEKCRSY 660

Query: 661 LAENLNKAALLELVVHESNKNSDRCGLALLVELLCLSRRTRITKLLDELKERSNGSSIMN 720
           LAENLNKA+LLEL+V E N N +RC +ALL ELLCL+RRT++ K LD L     G S M+
Sbjct: 661 LAENLNKASLLELIVLE-NHNCNRCAIALLTELLCLARRTQMMKFLDRLNNGWGGLSTMH 720

Query: 721 ILSVDLHRARPEEQPLVATILLQLDLMDDPLNCCSIFREEAIVTIITALNSQISREKTSE 780
           I    L RA  EE+PLVA ILLQLDL+ DP + CS++REEA+  + +A+N Q   EK  E
Sbjct: 721 IFLAYLQRASSEERPLVAAILLQLDLLGDPSH-CSMYREEAVDALTSAMNCQTCSEKIQE 780

Query: 781 NLARALLILGGRF-SCTGEPCPEIWLLQQAGFETNSGDSSYSSRHVYDDLVQSYEEEEDL 840
             ARALL+LGG F S   E   E WLL+ AGF  +S DS Y      D+ +   EEE+  
Sbjct: 781 KSARALLMLGGLFPSYIEEATSEKWLLKLAGFNEHSDDSFYGK----DENLN--EEEKAT 840

Query: 841 VNWQLKASTVFFNHGHKSLLSALSTSMTNGIPRLAKASLVTVSWMSRYLFVIRDEKLCSM 870
             WQ KA+      G K  L+AL+  M NG P LA+ASL TV+WMSR+L    DE   + 
Sbjct: 841 EIWQQKAAMALLKSGSKGFLAALANCMVNGTPSLARASLFTVAWMSRFLHSAVDENFLTT 900