Lsi02G009130 (gene) Bottle gourd (USVL1VR-Ls)

NameLsi02G009130
Typegene
OrganismLagenaria siceraria (Bottle gourd (USVL1VR-Ls))
DescriptionChitinase family protein
Locationchr02 : 8767013 .. 8769186 (+)
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: five_prime_UTRCDS
Hold the cursor over a type above to highlight its positions in the sequence below.
AAAAACAGAAATGGGTGGTGTTGTAGCCATGCAAAAGGCACTGCTCTTAACCGCCGTAGCTCTAATCGGAATCATCGCCGGAGCCGCAGCACAGAACTGCGGGTGCGCGGCGGGGCTGTGCTGCAGCAGATTCGGATTCTGCGGGACCGGTGATGATTTCTGTGGGAGTGGATGCACAGAAGGGCCGTGCAATATTCCCCCACTTACCCCTAGTGTGAACGACGTGGTTGTGGCGGATTTTGTGACGGAGGAATTTTTCAACGGCATAATTAACCAGGCGGACGCGGGTTGCGCCGGCCGGGGATTCTACAGTAGAGCTACTTTCTTAGAAGCTCTCCGATCGTTTGACCGATTTGGAAGAATTGGATCAGTTGACGATTCTAAAAGAGAAATCGCCGCCTTCTTTGCCCATGTTACTCACGAGACTGGACGTAAGCATTTTTTTTCTTCTTTTCCCTTTCTTCCTCTCTTGATTTTAAACTTAAATCAATCATTATTCCATTTCATTTAATTTGGATCATTATCTGAATTTTCATATTTGTGTTTATTAACTCCTTCAATTTTACAAAATATCTCTAAATTTTGGATCTAATTTTTTATTTAATTTTTAAGTTTCTAAAAGTATACATCTAGTTCTTAAGTTGTAAATTTGTAGTTCATAAGTTTTAAAATACATTGAGATTTTGAGTTTGGTTTTAATTTGGTCCCTAATTTTAAAATGTAACTTTGAATTTTCAATCAATACTTACTTTTCGTCTTTAGTATTAATGTTTATTAATTAATTTAAAAAAATTAAGAATTATAATAAATAAAATTTCACTATTTTTCGGATCCGTTTGGATTGACTAGAGAAAAAAAGTGTTTTTAAAAAAAAAAATTATTTTTATTTAAATTCTTTTGACAAAAACGACACTTTAAAAACTATTTGAAGTGGTTGTCAAACACTCCAATTTTTTTCAAAATGGCTTGTTTTTTAAATTAAACACTTAAAATACATTCCAAACACACCCTTCATTACTATTAAAATTAAATTTAAAATTTTATTTTGTATTTATTTTAAATTAATTAATAGATACTGACGTCAATGATTGATAGTGGGTATTTAATGAAAAAATAAGGTTTAAAAATCATGGTAAACTAAAAATCAAATAGTTATAAAATGAAACCATATATTATTACAACTTTCAGCCTATAAACTTTAAAATTTGTGTCTATTTAATCATTGAAATTTAAAACGTATATTCCCTAAAACTTTCAATTTTGTACGTAATAAGTTCGTAACAACTCAATTTTTTTTATAAATCAATTTATCTATTAGATATAAAATGGAACTTATGTCCAATAAAACCTTAAACTTTCCAATTGATGAATTAAAAAAAAAAAAAAAAATCTGAAACCATACGACTAAATTTGCACTTTTGAAAAGTTGAACAACCAAATAAATACAAACTCCAAAGTGTATAGACAAAATTTAAAATCTAATCAAATTAAATTTTATACTTAGATTCAAATTTTAATTTAGTGTTCACTAATTTATAGTTAAATGACAAATTTGGTCCCTTTGATTTGATAAAACTTCATAAATAGTCTTTATAGTTTGATAAAATTCTCGTAAATATGTAGTCCCTACTATAGATACTAGTTATGAAGCTTTTATCGAGATTGAATTCTAATTATAACCCATTTTTTCAAAATCATAGACACCAAATAAAAAAAGTTAAAGTTAATGACGAAAATTTAGAAATTTGATTTTGTATTGTACAACAGATTTGTGCTTCATAGAAGAAATAGATGGGGCAACAAAGGACTATTGCGACGAGGAAAACACTCAATATCCATGCAACCCAAGCAAGGGTTATTTCGGGAGAGGCCCAATCCAACTATCTTGGAACTTCAACTATGGGCCGGCAGGGGAAAACATCGGGTTCGACGGTCTAAACAACCCGGAAATCGTGGCCACCGACCCGGTGGTGTCCTTCAAGACCGCGCTCTGGTATTGGATGAACTTTGTGAGGCCGGTCATTAACCAAGGCTTCGGCGCCACCATTCGAGCCATCAATGGCGCCCTCGAATGCGACGGCGCTAACAATGCCACCGTTCAACGACGGGTCCAGTTCTATACTCAGTATTGTGATCAACTCGGCGTTGCCCCTGGGAATAATTTGACTTGTTAG

mRNA sequence

AAAAACAGAAATGGGTGGTGTTGTAGCCATGCAAAAGGCACTGCTCTTAACCGCCGTAGCTCTAATCGGAATCATCGCCGGAGCCGCAGCACAGAACTGCGGGTGCGCGGCGGGGCTGTGCTGCAGCAGATTCGGATTCTGCGGGACCGGTGATGATTTCTGTGGGAGTGGATGCACAGAAGGGCCGTGCAATATTCCCCCACTTACCCCTAGTGTGAACGACGTGGTTGTGGCGGATTTTGTGACGGAGGAATTTTTCAACGGCATAATTAACCAGGCGGACGCGGGTTGCGCCGGCCGGGGATTCTACAGTAGAGCTACTTTCTTAGAAGCTCTCCGATCGTTTGACCGATTTGGAAGAATTGGATCAGTTGACGATTCTAAAAGAGAAATCGCCGCCTTCTTTGCCCATGTTACTCACGAGACTGGACATTTGTGCTTCATAGAAGAAATAGATGGGGCAACAAAGGACTATTGCGACGAGGAAAACACTCAATATCCATGCAACCCAAGCAAGGGTTATTTCGGGAGAGGCCCAATCCAACTATCTTGGAACTTCAACTATGGGCCGGCAGGGGAAAACATCGGGTTCGACGGTCTAAACAACCCGGAAATCGTGGCCACCGACCCGGTGGTGTCCTTCAAGACCGCGCTCTGGTATTGGATGAACTTTGTGAGGCCGGTCATTAACCAAGGCTTCGGCGCCACCATTCGAGCCATCAATGGCGCCCTCGAATGCGACGGCGCTAACAATGCCACCGTTCAACGACGGGTCCAGTTCTATACTCAGTATTGTGATCAACTCGGCGTTGCCCCTGGGAATAATTTGACTTGTTAG

Coding sequence (CDS)

ATGGGTGGTGTTGTAGCCATGCAAAAGGCACTGCTCTTAACCGCCGTAGCTCTAATCGGAATCATCGCCGGAGCCGCAGCACAGAACTGCGGGTGCGCGGCGGGGCTGTGCTGCAGCAGATTCGGATTCTGCGGGACCGGTGATGATTTCTGTGGGAGTGGATGCACAGAAGGGCCGTGCAATATTCCCCCACTTACCCCTAGTGTGAACGACGTGGTTGTGGCGGATTTTGTGACGGAGGAATTTTTCAACGGCATAATTAACCAGGCGGACGCGGGTTGCGCCGGCCGGGGATTCTACAGTAGAGCTACTTTCTTAGAAGCTCTCCGATCGTTTGACCGATTTGGAAGAATTGGATCAGTTGACGATTCTAAAAGAGAAATCGCCGCCTTCTTTGCCCATGTTACTCACGAGACTGGACATTTGTGCTTCATAGAAGAAATAGATGGGGCAACAAAGGACTATTGCGACGAGGAAAACACTCAATATCCATGCAACCCAAGCAAGGGTTATTTCGGGAGAGGCCCAATCCAACTATCTTGGAACTTCAACTATGGGCCGGCAGGGGAAAACATCGGGTTCGACGGTCTAAACAACCCGGAAATCGTGGCCACCGACCCGGTGGTGTCCTTCAAGACCGCGCTCTGGTATTGGATGAACTTTGTGAGGCCGGTCATTAACCAAGGCTTCGGCGCCACCATTCGAGCCATCAATGGCGCCCTCGAATGCGACGGCGCTAACAATGCCACCGTTCAACGACGGGTCCAGTTCTATACTCAGTATTGTGATCAACTCGGCGTTGCCCCTGGGAATAATTTGACTTGTTAG

Protein sequence

MGGVVAMQKALLLTAVALIGIIAGAAAQNCGCAAGLCCSRFGFCGTGDDFCGSGCTEGPCNIPPLTPSVNDVVVADFVTEEFFNGIINQADAGCAGRGFYSRATFLEALRSFDRFGRIGSVDDSKREIAAFFAHVTHETGHLCFIEEIDGATKDYCDEENTQYPCNPSKGYFGRGPIQLSWNFNYGPAGENIGFDGLNNPEIVATDPVVSFKTALWYWMNFVRPVINQGFGATIRAINGALECDGANNATVQRRVQFYTQYCDQLGVAPGNNLTC
BLAST of Lsi02G009130 vs. Swiss-Prot
Match: CHI5_ARATH (Endochitinase EP3 OS=Arabidopsis thaliana GN=EP3 PE=1 SV=1)

HSP 1 Score: 414.1 bits (1063), Expect = 1.2e-114
Identity = 189/249 (75.90%), Postives = 210/249 (84.34%), Query Frame = 1

Query: 27  AQNCGCAAGLCCSRFGFCGTGDDFCGSGCTEGPCNIPPLTPSVNDVVVADFVTEEFFNGI 86
           AQNCGC++ LCCS+FGFCG   D+CG GC +GPC  PP     N V VA+ VT+EFFNGI
Sbjct: 28  AQNCGCSSELCCSQFGFCGNTSDYCGVGCQQGPCFAPP---PANGVSVAEIVTQEFFNGI 87

Query: 87  INQADAGCAGRGFYSRATFLEALRSFDRFGRIGSVDDSKREIAAFFAHVTHETGHLCFIE 146
           I+QA + CAG  FYSR  FLEAL S+ RFGR+GS DDS+REIAAFFAHVTHETGH C+IE
Sbjct: 88  ISQAASSCAGNRFYSRGAFLEALDSYSRFGRVGSTDDSRREIAAFFAHVTHETGHFCYIE 147

Query: 147 EIDGATKDYCDEENTQYPCNPSKGYFGRGPIQLSWNFNYGPAGENIGFDGLNNPEIVATD 206
           EIDGA+KDYCDE  TQYPCNP+KGY+GRGPIQLSWNFNYGPAG  IGFDGLN PE VATD
Sbjct: 148 EIDGASKDYCDENATQYPCNPNKGYYGRGPIQLSWNFNYGPAGTAIGFDGLNAPETVATD 207

Query: 207 PVVSFKTALWYWMNFVRPVINQGFGATIRAINGALECDGANNATVQRRVQFYTQYCDQLG 266
           PV+SFKTALWYW N V+PVI+QGFGATIRAINGALECDGAN ATVQ RV++YT YC QLG
Sbjct: 208 PVISFKTALWYWTNRVQPVISQGFGATIRAINGALECDGANTATVQARVRYYTDYCRQLG 267

Query: 267 VAPGNNLTC 276
           V PGNNLTC
Sbjct: 268 VDPGNNLTC 273

BLAST of Lsi02G009130 vs. Swiss-Prot
Match: CHI4_PHAVU (Endochitinase PR4 OS=Phaseolus vulgaris GN=CHI4 PE=2 SV=1)

HSP 1 Score: 391.7 bits (1005), Expect = 6.5e-108
Identity = 186/266 (69.92%), Postives = 212/266 (79.70%), Query Frame = 1

Query: 11  LLLTAVALIGIIAGAAAQNCGCAAGLCCSRFGFCGTGDDFCGSGCTEGPCNIPPLTPSVN 70
           L+L AVAL+      +AQNCGCA GLCCS++G+CGTG+D+CG+GC +GPC      PS N
Sbjct: 7   LVLVAVALVMGPKNVSAQNCGCAEGLCCSQYGYCGTGEDYCGTGCQQGPCTTASPPPSNN 66

Query: 71  DVVVADFVTEEFFNGIINQADAGCAGRGFYSRATFLEALRSFDRFGRIGSVDDSKREIAA 130
             V AD +T +F NGII+QAD+GCAG+ FY+R  FL AL S+  FGR+GS DDSKREIAA
Sbjct: 67  --VNADILTADFLNGIIDQADSGCAGKNFYTRDAFLSALNSYTDFGRVGSEDDSKREIAA 126

Query: 131 FFAHVTHETGHLCFIEEIDGATKDYCDEEN-TQYPCNPSKGYFGRGPIQLSWNFNYGPAG 190
            FAH THETGH C+IEEIDGA+KDYCDEE+  QYPC+ SKGY GRGPIQLSWNFNYGPAG
Sbjct: 127 AFAHFTHETGHFCYIEEIDGASKDYCDEESIAQYPCSSSKGYHGRGPIQLSWNFNYGPAG 186

Query: 191 ENIGFDGLNNPEIVATDPVVSFKTALWYWMNFVRPVINQGFGATIRAINGALECDGANNA 250
               FDGL  PE V+ D VVSFKTALWYWM  VRPVINQGFGATIRAINGALECDGAN  
Sbjct: 187 SANNFDGLGAPETVSNDVVVSFKTALWYWMQHVRPVINQGFGATIRAINGALECDGANPT 246

Query: 251 TVQRRVQFYTQYCDQLGVAPGNNLTC 276
           TVQ RV +YT+YC QLGVA G+NLTC
Sbjct: 247 TVQARVNYYTEYCRQLGVATGDNLTC 270

BLAST of Lsi02G009130 vs. Swiss-Prot
Match: CHI6_ORYSJ (Chitinase 6 OS=Oryza sativa subsp. japonica GN=Cht6 PE=2 SV=1)

HSP 1 Score: 352.4 bits (903), Expect = 4.3e-96
Identity = 164/275 (59.64%), Postives = 204/275 (74.18%), Query Frame = 1

Query: 5   VAMQKALLLTAVALIGIIAG--AAAQNCGCAAGLCCSRFGFCGTGDDFCGSGCTEGPCNI 64
           +A + +LL   +A++  ++   AAAQ+CGCA+  CCS++GFCGTG D+CG+GC  GPC++
Sbjct: 1   MARRLSLLAVVLAMVAAVSASTAAAQSCGCASDQCCSKWGFCGTGSDYCGTGCQAGPCDV 60

Query: 65  PPLTPSVNDVVVADFVTEEFFNGIINQADAGCAGRGFYSRATFLEALRSFDRFGRIGSVD 124
           P    + NDV VA  VT EFF  ++ QAD GCA +GFY+R  FL A   +  FGR GSVD
Sbjct: 61  P----ATNDVSVASIVTPEFFAALVAQADDGCAAKGFYTRDAFLTAAGGYPSFGRTGSVD 120

Query: 125 DSKREIAAFFAHVTHETGHLCFIEEIDGATKDYCDEENTQYPCNPSKGYFGRGPIQLSWN 184
           DSKREIAAFFAH  HET   C+IEEIDG +K+YCDE +TQ+PC   KGY+GRGP+Q+SWN
Sbjct: 121 DSKREIAAFFAHANHETIKFCYIEEIDGPSKNYCDETSTQWPCMAGKGYYGRGPLQISWN 180

Query: 185 FNYGPAGENIGFDGLNNPEIVATDPVVSFKTALWYWMNFVRP--VINQGFGATIRAINGA 244
           FNYGPAG++IGFDGL +P+ VA  PV++F+TALWYW N V    V  QGFGATIRAINGA
Sbjct: 181 FNYGPAGQSIGFDGLGDPDAVARSPVLAFQTALWYWTNNVHDAFVSGQGFGATIRAINGA 240

Query: 245 LECDGANNATVQRRVQFYTQYCDQLGVAPGNNLTC 276
           LECDG N   V  RV +Y Q+C Q GV PG+NLTC
Sbjct: 241 LECDGKNPTAVSNRVAYYQQFCQQFGVDPGSNLTC 271

BLAST of Lsi02G009130 vs. Swiss-Prot
Match: CHI4_ORYSJ (Chitinase 4 OS=Oryza sativa subsp. japonica GN=Cht4 PE=2 SV=2)

HSP 1 Score: 342.0 bits (876), Expect = 5.9e-93
Identity = 163/285 (57.19%), Postives = 195/285 (68.42%), Query Frame = 1

Query: 1   MGGVVAMQKALLLTAVALIGIIAGAAAQNCGCAAGLCCSRFGFCGTGDDFCGSGCTEGPC 60
           M   +A   AL+     L+   A AAAQNCGC  G CCS++G+CGT + +CG GC  GPC
Sbjct: 1   MAAKMATMVALVFGLALLLSAAAPAAAQNCGCQDGYCCSQWGYCGTTEAYCGQGCQSGPC 60

Query: 61  ----------NIPPLTPSVNDVVVADFVTEEFFNGIINQADAGCAGRGFYSRATFLEALR 120
                            +   V V   VTE FFNGI NQA  GCAG+ FY+R +FL A R
Sbjct: 61  WGSGGEAAAGMAGRKAGAGAGVSVESVVTEAFFNGIKNQAPNGCAGKSFYTRQSFLNAAR 120

Query: 121 SFDRFGRIGSVDDSKREIAAFFAHVTHETGHLCFIEEIDGATKDYCDEENTQYPCNPSKG 180
           S+  F    + DDSKREIAAFFAHVTHETGH+C+I EI+GA  DYCD+ N Q+PC P K 
Sbjct: 121 SYSGFANDRTNDDSKREIAAFFAHVTHETGHMCYINEINGANMDYCDKSNKQWPCQPGKK 180

Query: 181 YFGRGPIQLSWNFNYGPAGENIGFDGLNNPEIVATDPVVSFKTALWYWMNFVRPVINQGF 240
           Y+GRGP+Q+SWNFNYGPAG+NIGFDGL +P+ VA DP +SFKTALW+WMN V  V++QGF
Sbjct: 181 YYGRGPLQISWNFNYGPAGKNIGFDGLRDPDKVAQDPTISFKTALWFWMNNVHQVMSQGF 240

Query: 241 GATIRAINGALECDGANNATVQRRVQFYTQYCDQLGVAPGNNLTC 276
           GATIRAINGALEC+G N   V  RV +Y  YC Q GV+PG NL C
Sbjct: 241 GATIRAINGALECNGKNPGAVNARVNYYKDYCRQFGVSPGGNLYC 285

BLAST of Lsi02G009130 vs. Swiss-Prot
Match: CHI5_ORYSJ (Chitinase 5 OS=Oryza sativa subsp. japonica GN=Cht5 PE=2 SV=1)

HSP 1 Score: 339.0 bits (868), Expect = 5.0e-92
Identity = 162/282 (57.45%), Postives = 196/282 (69.50%), Query Frame = 1

Query: 5   VAMQKALLLTAVALIGIIAGAAAQNCGCAAGLCCSRFGFCGTGDDFCGSGCTEGPC---- 64
           +A+  ALLL+A         A+AQNCGC + +CCS++G+CGTG D+CG GC  GPC    
Sbjct: 13  LALGLALLLSATGQ------ASAQNCGCQSNMCCSKWGYCGTGKDYCGDGCRSGPCYGGG 72

Query: 65  -------NIPPLTPSVNDVVVADFVTEEFFNGIINQADAGCAGRGFYSRATFLEALRSFD 124
                           + V V   VTE FFNGI NQA  GCAG+ FY+R +FL A  S+ 
Sbjct: 73  GGGGGGGGGGGGGGGGSGVSVESVVTEAFFNGIKNQAPNGCAGKNFYTRQSFLNAAHSYS 132

Query: 125 RFGRIGSVDDSKREIAAFFAHVTHETGHLCFIEEIDGATKDYCDEENTQYPCNPSKGYFG 184
            F R  + DDSKREIAAFFAHVTHETGH+C+I EI+GA+ DYCD+ N Q+PC P K Y+G
Sbjct: 133 GFARDRTNDDSKREIAAFFAHVTHETGHMCYINEINGASMDYCDKNNKQWPCQPGKKYYG 192

Query: 185 RGPIQLSWNFNYGPAGENIGFDGLNNPEIVATDPVVSFKTALWYWMNFVRPVINQGFGAT 244
           RGP+Q+SWN+NYGPAG+NIGFDGL +P+ VA DP +SFKTALW+WMN V  V+ QGFGAT
Sbjct: 193 RGPLQISWNYNYGPAGQNIGFDGLRDPDRVAQDPTISFKTALWFWMNNVHQVMLQGFGAT 252

Query: 245 IRAINGALECDGANNATVQRRVQFYTQYCDQLGVAPGNNLTC 276
           IRAINGALEC+G N   V  RV +Y  YC Q GV PG NL C
Sbjct: 253 IRAINGALECNGKNPGAVNARVNYYKDYCRQFGVDPGGNLYC 288

BLAST of Lsi02G009130 vs. TrEMBL
Match: A0A0A0LXT8_CUCSA (Uncharacterized protein OS=Cucumis sativus GN=Csa_1G534750 PE=3 SV=1)

HSP 1 Score: 541.2 bits (1393), Expect = 7.3e-151
Identity = 253/271 (93.36%), Postives = 264/271 (97.42%), Query Frame = 1

Query: 5   VAMQKALLLTAVALIGIIAGAAAQNCGCAAGLCCSRFGFCGTGDDFCGSGCTEGPCNIPP 64
           VAMQK LLLTA+ALIGIIAGAAAQNCGCAAGLCCSRFGFCG G+DFCG+GC EGPCNIPP
Sbjct: 8   VAMQKTLLLTALALIGIIAGAAAQNCGCAAGLCCSRFGFCGNGEDFCGTGCREGPCNIPP 67

Query: 65  LTPSVNDVVVADFVTEEFFNGIINQADAGCAGRGFYSRATFLEALRSFDRFGRIGSVDDS 124
           LTPSVNDV VA+FVTEEFFNGIIN+ADAGCAGRGFYSRATFLEAL+SFDRFGRIGSV+DS
Sbjct: 68  LTPSVNDVNVAEFVTEEFFNGIINEADAGCAGRGFYSRATFLEALQSFDRFGRIGSVEDS 127

Query: 125 KREIAAFFAHVTHETGHLCFIEEIDGATKDYCDEENTQYPCNPSKGYFGRGPIQLSWNFN 184
           KREIAAFFAHVTHETGHLCFIEEIDGAT+DYCDEENTQYPCNPSKGYFGRGPIQLSWNFN
Sbjct: 128 KREIAAFFAHVTHETGHLCFIEEIDGATRDYCDEENTQYPCNPSKGYFGRGPIQLSWNFN 187

Query: 185 YGPAGENIGFDGLNNPEIVATDPVVSFKTALWYWMNFVRPVINQGFGATIRAINGALECD 244
           YGPAGENIGFDGLNNPEIVAT+PVVSFKTALWYWMNFVRPVINQGFGATIRAINGALECD
Sbjct: 188 YGPAGENIGFDGLNNPEIVATNPVVSFKTALWYWMNFVRPVINQGFGATIRAINGALECD 247

Query: 245 GANNATVQRRVQFYTQYCDQLGVAPGNNLTC 276
           GAN ATVQRR+QFY QYC+QL VAPGNNLTC
Sbjct: 248 GANTATVQRRIQFYNQYCNQLNVAPGNNLTC 278

BLAST of Lsi02G009130 vs. TrEMBL
Match: B9VQ34_PYRPY (Class IV chitinase OS=Pyrus pyrifolia PE=2 SV=1)

HSP 1 Score: 453.0 bits (1164), Expect = 2.6e-124
Identity = 206/272 (75.74%), Postives = 237/272 (87.13%), Query Frame = 1

Query: 5   VAMQKALLLTAVALIGIIAGAA-AQNCGCAAGLCCSRFGFCGTGDDFCGSGCTEGPCNIP 64
           +A +  +L   +   G+++G A AQNCGCAA LCCS++G+CGT D +CG GC  GPC  P
Sbjct: 1   MAARSIILTLFIVATGVLSGYADAQNCGCAADLCCSQYGYCGTDDAYCGKGCQSGPCKTP 60

Query: 65  PLTPSVNDVVVADFVTEEFFNGIINQADAGCAGRGFYSRATFLEALRSFDRFGRIGSVDD 124
             TPS NDVVVAD VT EFFNGII+QADA CAG+ FYSRATFLEAL S+++FGRIGSVDD
Sbjct: 61  AQTPSTNDVVVADIVTPEFFNGIIDQADASCAGKNFYSRATFLEALNSYNQFGRIGSVDD 120

Query: 125 SKREIAAFFAHVTHETGHLCFIEEIDGATKDYCDEENTQYPCNPSKGYFGRGPIQLSWNF 184
           SKREIAAFFAHVTHETGH C+IEEIDGA+KDYCDE NTQYPCNP+KGY+GRGPIQLSWNF
Sbjct: 121 SKREIAAFFAHVTHETGHFCYIEEIDGASKDYCDENNTQYPCNPNKGYYGRGPIQLSWNF 180

Query: 185 NYGPAGENIGFDGLNNPEIVATDPVVSFKTALWYWMNFVRPVINQGFGATIRAINGALEC 244
           NYGPAGE+IGFDGLN+PE VA DPV++FKTALWYWMN VRPVI +GFGATIRAINGALEC
Sbjct: 181 NYGPAGESIGFDGLNSPETVANDPVIAFKTALWYWMNNVRPVIGEGFGATIRAINGALEC 240

Query: 245 DGANNATVQRRVQFYTQYCDQLGVAPGNNLTC 276
           DG N ATVQ+RV++YT+YC+QLGVAPG+NLTC
Sbjct: 241 DGGNPATVQKRVEYYTEYCNQLGVAPGDNLTC 272

BLAST of Lsi02G009130 vs. TrEMBL
Match: A0A077GZM8_9ROSA (Chitinase ppl-2 OS=Pyrus x bretschneideri PE=2 SV=1)

HSP 1 Score: 453.0 bits (1164), Expect = 2.6e-124
Identity = 206/272 (75.74%), Postives = 237/272 (87.13%), Query Frame = 1

Query: 5   VAMQKALLLTAVALIGIIAGAA-AQNCGCAAGLCCSRFGFCGTGDDFCGSGCTEGPCNIP 64
           +A +  +L   +   G+++G A AQNCGCAA LCCS++G+CGT D +CG GC  GPC  P
Sbjct: 1   MAARSIILTLFIVATGVLSGYADAQNCGCAADLCCSQYGYCGTDDAYCGKGCQSGPCKTP 60

Query: 65  PLTPSVNDVVVADFVTEEFFNGIINQADAGCAGRGFYSRATFLEALRSFDRFGRIGSVDD 124
             TPS NDVVVAD VT EFFNGII+QADA CAG+ FYSRATFLEAL S+++FGRIGSVDD
Sbjct: 61  AQTPSTNDVVVADIVTPEFFNGIIDQADASCAGKNFYSRATFLEALNSYNQFGRIGSVDD 120

Query: 125 SKREIAAFFAHVTHETGHLCFIEEIDGATKDYCDEENTQYPCNPSKGYFGRGPIQLSWNF 184
           SKREIAAFFAHVTHETGH C+IEEIDGA+KDYCDE NTQYPCNP+KGY+GRGPIQLSWNF
Sbjct: 121 SKREIAAFFAHVTHETGHFCYIEEIDGASKDYCDENNTQYPCNPNKGYYGRGPIQLSWNF 180

Query: 185 NYGPAGENIGFDGLNNPEIVATDPVVSFKTALWYWMNFVRPVINQGFGATIRAINGALEC 244
           NYGPAGE+IGFDGLN+PE VA DPV++FKTALWYWMN VRPVI +GFGATIRAINGALEC
Sbjct: 181 NYGPAGESIGFDGLNSPETVANDPVIAFKTALWYWMNNVRPVIGEGFGATIRAINGALEC 240

Query: 245 DGANNATVQRRVQFYTQYCDQLGVAPGNNLTC 276
           DG N ATVQ+RV++YT+YC+QLGVAPG+NLTC
Sbjct: 241 DGGNPATVQKRVEYYTEYCNQLGVAPGDNLTC 272

BLAST of Lsi02G009130 vs. TrEMBL
Match: A0A059DAK6_EUCGR (Uncharacterized protein OS=Eucalyptus grandis GN=EUGRSUZ_A00020 PE=3 SV=1)

HSP 1 Score: 450.7 bits (1158), Expect = 1.3e-123
Identity = 210/272 (77.21%), Postives = 236/272 (86.76%), Query Frame = 1

Query: 9   KALLLTAVALIGIIAGA-----AAQNCGCAAGLCCSRFGFCGTGDDFCGSGCTEGPCNIP 68
           + LLLTAVA+ GII  A     +AQNCGCAA  CCSR+G+CGTGDD+CG+GC EGPCN P
Sbjct: 7   RKLLLTAVAVAGIIISALPDTTSAQNCGCAADQCCSRWGYCGTGDDYCGTGCQEGPCNAP 66

Query: 69  PLTPSVNDVVVADFVTEEFFNGIINQADAGCAGRGFYSRATFLEALRSFDRFGRIGSVDD 128
           P T   NDVVVAD VTE FF+GII QA   C G+GFYSR TFLEA  S+ +FGR+GSVDD
Sbjct: 67  PPT---NDVVVADVVTEAFFDGIIGQAAESCEGKGFYSRGTFLEAAGSYPQFGRVGSVDD 126

Query: 129 SKREIAAFFAHVTHETGHLCFIEEIDGATKDYCDEENTQYPCNPSKGYFGRGPIQLSWNF 188
           SKREIAAFFAHVTHETGH C+I EIDGA+KDYCDE NTQYPC+P+KGY+GRGPIQLSWNF
Sbjct: 127 SKREIAAFFAHVTHETGHFCYINEIDGASKDYCDETNTQYPCSPNKGYYGRGPIQLSWNF 186

Query: 189 NYGPAGENIGFDGLNNPEIVATDPVVSFKTALWYWMNFVRPVINQGFGATIRAINGALEC 248
           NYGPAGE+IGFDGLN+P+ VATDP++SFKTALWYWMNFVRPVI+QGFGATIRAINGALEC
Sbjct: 187 NYGPAGESIGFDGLNSPDTVATDPLISFKTALWYWMNFVRPVIDQGFGATIRAINGALEC 246

Query: 249 DGANNATVQRRVQFYTQYCDQLGVAPGNNLTC 276
           DG N ATVQ RVQ+YT+YC QLGVAPG+NLTC
Sbjct: 247 DGGNPATVQARVQYYTEYCSQLGVAPGDNLTC 275

BLAST of Lsi02G009130 vs. TrEMBL
Match: A0A061ER07_THECC (Carrot EP3-3 chitinase OS=Theobroma cacao GN=TCM_019914 PE=3 SV=1)

HSP 1 Score: 446.0 bits (1146), Expect = 3.2e-122
Identity = 204/266 (76.69%), Postives = 232/266 (87.22%), Query Frame = 1

Query: 13  LTAVALIGIIAGAA---AQNCGCAAGLCCSRFGFCGTGDDFCGSGCTEGPCNIPPLTPSV 72
           L A+ L GIIAGAA   AQNCGCA GLCCSR+GFCGTGDDFCG+GC EGPCN PP   ++
Sbjct: 11  LLAIVLAGIIAGAAPVKAQNCGCAEGLCCSRWGFCGTGDDFCGTGCQEGPCNPPP---TL 70

Query: 73  NDVVVADFVTEEFFNGIINQADAGCAGRGFYSRATFLEALRSFDRFGRIGSVDDSKREIA 132
           N+V VAD VT +FFNGI+++A+A C G+ FYSRA FLEAL S+ +FGRIGS+DDSKREIA
Sbjct: 71  NNVSVADIVTPQFFNGILDEAEASCEGKNFYSRAAFLEALNSYPQFGRIGSIDDSKREIA 130

Query: 133 AFFAHVTHETGHLCFIEEIDGATKDYCDEENTQYPCNPSKGYFGRGPIQLSWNFNYGPAG 192
           AFF HVTHETGH CFIEEI+G T+DYCDE +TQYPCNP KGY+GRGPIQLSWNFNYGPAG
Sbjct: 131 AFFGHVTHETGHFCFIEEINGTTRDYCDESSTQYPCNPDKGYYGRGPIQLSWNFNYGPAG 190

Query: 193 ENIGFDGLNNPEIVATDPVVSFKTALWYWMNFVRPVINQGFGATIRAINGALECDGANNA 252
           E+IGFDGLN+PE VATDPV+SFKTALWYW+NFV+PVI QGFGATIRAINGALECDG N A
Sbjct: 191 ESIGFDGLNSPETVATDPVISFKTALWYWVNFVQPVIGQGFGATIRAINGALECDGGNPA 250

Query: 253 TVQRRVQFYTQYCDQLGVAPGNNLTC 276
           TVQ R+++YT YC+QLGV PG NLTC
Sbjct: 251 TVQARIEYYTDYCNQLGVDPGANLTC 273

BLAST of Lsi02G009130 vs. TAIR10
Match: AT3G54420.1 (AT3G54420.1 homolog of carrot EP3-3 chitinase)

HSP 1 Score: 414.1 bits (1063), Expect = 6.9e-116
Identity = 189/249 (75.90%), Postives = 210/249 (84.34%), Query Frame = 1

Query: 27  AQNCGCAAGLCCSRFGFCGTGDDFCGSGCTEGPCNIPPLTPSVNDVVVADFVTEEFFNGI 86
           AQNCGC++ LCCS+FGFCG   D+CG GC +GPC  PP     N V VA+ VT+EFFNGI
Sbjct: 28  AQNCGCSSELCCSQFGFCGNTSDYCGVGCQQGPCFAPP---PANGVSVAEIVTQEFFNGI 87

Query: 87  INQADAGCAGRGFYSRATFLEALRSFDRFGRIGSVDDSKREIAAFFAHVTHETGHLCFIE 146
           I+QA + CAG  FYSR  FLEAL S+ RFGR+GS DDS+REIAAFFAHVTHETGH C+IE
Sbjct: 88  ISQAASSCAGNRFYSRGAFLEALDSYSRFGRVGSTDDSRREIAAFFAHVTHETGHFCYIE 147

Query: 147 EIDGATKDYCDEENTQYPCNPSKGYFGRGPIQLSWNFNYGPAGENIGFDGLNNPEIVATD 206
           EIDGA+KDYCDE  TQYPCNP+KGY+GRGPIQLSWNFNYGPAG  IGFDGLN PE VATD
Sbjct: 148 EIDGASKDYCDENATQYPCNPNKGYYGRGPIQLSWNFNYGPAGTAIGFDGLNAPETVATD 207

Query: 207 PVVSFKTALWYWMNFVRPVINQGFGATIRAINGALECDGANNATVQRRVQFYTQYCDQLG 266
           PV+SFKTALWYW N V+PVI+QGFGATIRAINGALECDGAN ATVQ RV++YT YC QLG
Sbjct: 208 PVISFKTALWYWTNRVQPVISQGFGATIRAINGALECDGANTATVQARVRYYTDYCRQLG 267

Query: 267 VAPGNNLTC 276
           V PGNNLTC
Sbjct: 268 VDPGNNLTC 273

BLAST of Lsi02G009130 vs. TAIR10
Match: AT2G43590.1 (AT2G43590.1 Chitinase family protein)

HSP 1 Score: 317.4 bits (812), Expect = 8.7e-87
Identity = 152/272 (55.88%), Postives = 191/272 (70.22%), Query Frame = 1

Query: 5   VAMQKALLLTAVALIGIIAGAA-AQNCGCAAGLCCSRFGFCGTGDDFCGSGCTEGPCNIP 64
           +A  K  L+  + L+G  +    +QNCGCA  LCCS+FG+CGT D +CG GC  GPC   
Sbjct: 1   MAFTKISLVLLLCLLGFFSETVKSQNCGCAPNLCCSQFGYCGTDDAYCGVGCRSGPCRGS 60

Query: 65  PLTPSVNDVVVADFVTEEFFNGIINQADAGCAGRGFYSRATFLEALRSFDRFGRIGSVDD 124
             TP+   V     VT+ FFN IINQA  GCAG+ FY+R +F+ A  +F  F    SV  
Sbjct: 61  G-TPTGGSV--GSIVTQGFFNNIINQAGNGCAGKRFYTRDSFVNAANTFPNFAN--SV-- 120

Query: 125 SKREIAAFFAHVTHETGHLCFIEEIDGATKDYCDEENTQYPCNPSKGYFGRGPIQLSWNF 184
           ++REIA  FAH THETGH C+IEEI+GAT++YC   NTQYPC P KGYFGRGPIQLSWN+
Sbjct: 121 TRREIATMFAHFTHETGHFCYIEEINGATRNYCQSSNTQYPCAPGKGYFGRGPIQLSWNY 180

Query: 185 NYGPAGENIGFDGLNNPEIVATDPVVSFKTALWYWMNFVRPVINQGFGATIRAINGALEC 244
           NYG  G+++G D L  PE+V ++P V+F+T LW+WMN VRPV+NQGFGATIRAING +EC
Sbjct: 181 NYGACGQSLGLDLLRQPELVGSNPTVAFRTGLWFWMNSVRPVLNQGFGATIRAING-MEC 240

Query: 245 DGANNATVQRRVQFYTQYCDQLGVAPGNNLTC 276
           +G N+  V  R+ +Y  YC QLGV PG NL+C
Sbjct: 241 NGGNSGAVNARIGYYRDYCGQLGVDPGPNLSC 264

BLAST of Lsi02G009130 vs. TAIR10
Match: AT2G43580.1 (AT2G43580.1 Chitinase family protein)

HSP 1 Score: 302.0 bits (772), Expect = 3.8e-82
Identity = 141/272 (51.84%), Postives = 189/272 (69.49%), Query Frame = 1

Query: 5   VAMQKALLLTAVALIGIIAGAA-AQNCGCAAGLCCSRFGFCGTGDDFCGSGCTEGPCNIP 64
           +A+ K  L+  ++L+G+ +    +QNC CA  LCCS+FG+CGT  D+CGS C  GPC + 
Sbjct: 1   MALTKIFLILLLSLLGLYSETVKSQNCDCAPNLCCSQFGYCGTTADYCGSTCQSGPCRVG 60

Query: 65  PLTPSVNDVVVADFVTEEFFNGIINQADAGCAGRGFYSRATFLEALRSFDRFGRIGSVDD 124
              P     +V + VT+ FFN IINQA  GCAG+ FY+R +F+ A  +F  F    +   
Sbjct: 61  G--PPTGAGLVGNIVTQIFFNNIINQAGNGCAGKSFYTRDSFINATNTFPSFANTVT--- 120

Query: 125 SKREIAAFFAHVTHETGHLCFIEEIDGATKDYCDEENTQYPCNPSKGYFGRGPIQLSWNF 184
            +REIA  FAH T+ETGH C+IEEI+GA++  CD+ N QYPC P+K Y GRGP+ LSWNF
Sbjct: 121 -RREIATMFAHFTYETGHFCYIEEINGASRVMCDQNNRQYPCAPAKSYHGRGPLLLSWNF 180

Query: 185 NYGPAGENIGFDGLNNPEIVATDPVVSFKTALWYWMNFVRPVINQGFGATIRAINGALEC 244
           NYG  G+++G D L  PE+V+++PVV+F+TALW+WM  VRPV+NQGFGATIRAI+G  +C
Sbjct: 181 NYGACGQSLGLDLLRQPELVSSNPVVAFRTALWFWMKSVRPVLNQGFGATIRAISG-FDC 240

Query: 245 DGANNATVQRRVQFYTQYCDQLGVAPGNNLTC 276
           DG N   V  R+ +Y  YC QLG+ PG N+TC
Sbjct: 241 DGRNLGGVNARIGYYRDYCGQLGLDPGANITC 265

BLAST of Lsi02G009130 vs. TAIR10
Match: AT2G43570.1 (AT2G43570.1 chitinase, putative)

HSP 1 Score: 261.9 bits (668), Expect = 4.4e-70
Identity = 130/257 (50.58%), Postives = 169/257 (65.76%), Query Frame = 1

Query: 26  AAQNCGCAAGLCCSRFGFCGTGDDFCGSGCTEGPCNIPPL--TPSVNDVVVADFVTEEFF 85
           A+QNCGCA+  CCS++G+CGT D+FCG GC  GPC        P+V+   +   VT +FF
Sbjct: 30  ASQNCGCASDFCCSKYGYCGTTDEFCGEGCQAGPCRSSGGGGDPAVS---LEGTVTPDFF 89

Query: 86  NGIINQADAGCAGRGFYSRATFLEALRSFDRFGRIGSVDDSKREIAAFFAHVTHETGHLC 145
           N I+NQ    C G+GFY+  TF+ A  S+  FG       SKREIAAFFAHV  ETG +C
Sbjct: 90  NSILNQR-GDCPGKGFYTHDTFMAAANSYPSFG----ASISKREIAAFFAHVAQETGFMC 149

Query: 146 FIEEIDGATK----DYCDEENTQYPCNPSKGYFGRGPIQLSWNFNYGPAGENIGFDGLNN 205
           +IEEIDG  K    +YCD E  ++PC   KGY+GRG IQLSWN+NYG  G+ +  + L +
Sbjct: 150 YIEEIDGPAKAASGEYCDTEKPEFPCAQGKGYYGRGAIQLSWNYNYGLCGKALDENLLAS 209

Query: 206 PEIVATDPVVSFKTALWYWMNFVRPVINQGFGATIRAINGALECDGANN-ATVQRRVQFY 265
           PE VA D V++FKTA W+W   VR     GFGATIRA+N + EC G ++ A    R++++
Sbjct: 210 PEKVAQDQVLAFKTAFWFWTTNVRTSFKSGFGATIRAVN-SRECSGGDSTAKAANRIKYF 269

Query: 266 TQYCDQLGVAPGNNLTC 276
             YC +LGVAPG+NLTC
Sbjct: 270 QDYCGKLGVAPGDNLTC 277

BLAST of Lsi02G009130 vs. TAIR10
Match: AT2G43610.1 (AT2G43610.1 Chitinase family protein)

HSP 1 Score: 249.2 bits (635), Expect = 2.9e-66
Identity = 128/282 (45.39%), Postives = 175/282 (62.06%), Query Frame = 1

Query: 7   MQKALLLTAVALIGIIAGAAAQNCG---CAAGLCCSRFGFCGTGDDFCGSGCTEGPCNIP 66
           ++KAL++    L  +   A +QNCG   C   +CCSR+G+CGT   +CG+GC  GPCN  
Sbjct: 8   LKKALIIFLFTLTIMTGTAFSQNCGTNGCKGNMCCSRWGYCGTTKAYCGTGCQSGPCNSK 67

Query: 67  PL---TPSVNDVV-------VADFVTEEFFNGIINQADAGCAGRGFYSRATFLEALRSFD 126
           P    TPS +  +       +A  +T  FFN I+++  +GC  +GFY+R  F+ A  SF 
Sbjct: 68  PKPTPTPSGSGGLNAGPRGTIASVITPAFFNSIMSKVGSGCPAKGFYTRQAFIAAAESFA 127

Query: 127 RFGRIGSVDDSKREIAAFFAHVTHETGHLCFIEEIDGATKDYCDEENTQYPCNPSKGYFG 186
            +   G+V  +KREIAA  A  +HE+G  C+ EEI  A   YC    T YPC P K Y+G
Sbjct: 128 AYK--GTV--AKREIAAMLAQFSHESGSFCYKEEI--ARGRYCSPSTT-YPCQPGKNYYG 187

Query: 187 RGPIQLSWNFNYGPAGENIGFDGLNNPEIVATDPVVSFKTALWYWMNFVRPVINQGFGAT 246
           RGPIQ++WN+NYG AG+ +G   L +P++VA  P V+F+ A+W+W   VRPV++QGFGAT
Sbjct: 188 RGPIQITWNYNYGAAGKFLGLPLLKDPDMVARSPTVAFQCAMWFWNKNVRPVLSQGFGAT 247

Query: 247 IRAINGALECDGANNATVQRRVQFYTQYCDQLGVAPGNNLTC 276
            R ING  EC+G   A VQ RV  Y  +C +LGV PG NL+C
Sbjct: 248 TRRINGG-ECNGGRPAAVQSRVNHYLDFCKKLGVTPGTNLSC 281

BLAST of Lsi02G009130 vs. NCBI nr
Match: gi|659068474|ref|XP_008444611.1| (PREDICTED: endochitinase PR4-like [Cucumis melo])

HSP 1 Score: 542.0 bits (1395), Expect = 6.2e-151
Identity = 256/275 (93.09%), Postives = 264/275 (96.00%), Query Frame = 1

Query: 1   MGGVVAMQKALLLTAVALIGIIAGAAAQNCGCAAGLCCSRFGFCGTGDDFCGSGCTEGPC 60
           M G VAMQK LLLTAVALIGIIAGAAAQNCGCAAGLCCSRFGFCGTG+DFCG+GC EGPC
Sbjct: 1   MEGGVAMQKTLLLTAVALIGIIAGAAAQNCGCAAGLCCSRFGFCGTGEDFCGTGCREGPC 60

Query: 61  NIPPLTPSVNDVVVADFVTEEFFNGIINQADAGCAGRGFYSRATFLEALRSFDRFGRIGS 120
           NIPPLTPSVNDV VA+FVTEEFFNGIINQADA CAGRGFYSRATFLEAL+SFDRFGRIG+
Sbjct: 61  NIPPLTPSVNDVNVAEFVTEEFFNGIINQADASCAGRGFYSRATFLEALQSFDRFGRIGT 120

Query: 121 VDDSKREIAAFFAHVTHETGHLCFIEEIDGATKDYCDEENTQYPCNPSKGYFGRGPIQLS 180
           VDDSKREIAAFFAHVTHETGH CFIEEIDGAT+DYCDEENTQYPCNPSKGYFGRGPIQLS
Sbjct: 121 VDDSKREIAAFFAHVTHETGHFCFIEEIDGATRDYCDEENTQYPCNPSKGYFGRGPIQLS 180

Query: 181 WNFNYGPAGENIGFDGLNNPEIVATDPVVSFKTALWYWMNFVRPVINQGFGATIRAINGA 240
           WNFNYGPAGENIGFDGLNNPEIVAT+PVVSFKTALWYWMNFVRPVINQGFGATIRAINGA
Sbjct: 181 WNFNYGPAGENIGFDGLNNPEIVATNPVVSFKTALWYWMNFVRPVINQGFGATIRAINGA 240

Query: 241 LECDGANNATVQRRVQFYTQYCDQLGVAPGNNLTC 276
           LECDGAN ATVQRRVQFY QYC+QL V PGNNLTC
Sbjct: 241 LECDGANTATVQRRVQFYNQYCNQLNVDPGNNLTC 275

BLAST of Lsi02G009130 vs. NCBI nr
Match: gi|449454971|ref|XP_004145227.1| (PREDICTED: endochitinase PR4-like [Cucumis sativus])

HSP 1 Score: 541.2 bits (1393), Expect = 1.1e-150
Identity = 253/271 (93.36%), Postives = 264/271 (97.42%), Query Frame = 1

Query: 5   VAMQKALLLTAVALIGIIAGAAAQNCGCAAGLCCSRFGFCGTGDDFCGSGCTEGPCNIPP 64
           VAMQK LLLTA+ALIGIIAGAAAQNCGCAAGLCCSRFGFCG G+DFCG+GC EGPCNIPP
Sbjct: 8   VAMQKTLLLTALALIGIIAGAAAQNCGCAAGLCCSRFGFCGNGEDFCGTGCREGPCNIPP 67

Query: 65  LTPSVNDVVVADFVTEEFFNGIINQADAGCAGRGFYSRATFLEALRSFDRFGRIGSVDDS 124
           LTPSVNDV VA+FVTEEFFNGIIN+ADAGCAGRGFYSRATFLEAL+SFDRFGRIGSV+DS
Sbjct: 68  LTPSVNDVNVAEFVTEEFFNGIINEADAGCAGRGFYSRATFLEALQSFDRFGRIGSVEDS 127

Query: 125 KREIAAFFAHVTHETGHLCFIEEIDGATKDYCDEENTQYPCNPSKGYFGRGPIQLSWNFN 184
           KREIAAFFAHVTHETGHLCFIEEIDGAT+DYCDEENTQYPCNPSKGYFGRGPIQLSWNFN
Sbjct: 128 KREIAAFFAHVTHETGHLCFIEEIDGATRDYCDEENTQYPCNPSKGYFGRGPIQLSWNFN 187

Query: 185 YGPAGENIGFDGLNNPEIVATDPVVSFKTALWYWMNFVRPVINQGFGATIRAINGALECD 244
           YGPAGENIGFDGLNNPEIVAT+PVVSFKTALWYWMNFVRPVINQGFGATIRAINGALECD
Sbjct: 188 YGPAGENIGFDGLNNPEIVATNPVVSFKTALWYWMNFVRPVINQGFGATIRAINGALECD 247

Query: 245 GANNATVQRRVQFYTQYCDQLGVAPGNNLTC 276
           GAN ATVQRR+QFY QYC+QL VAPGNNLTC
Sbjct: 248 GANTATVQRRIQFYNQYCNQLNVAPGNNLTC 278

BLAST of Lsi02G009130 vs. NCBI nr
Match: gi|222139394|gb|ACM45716.1| (class IV chitinase [Pyrus x bretschneideri x Pyrus pyrifolia])

HSP 1 Score: 453.0 bits (1164), Expect = 3.8e-124
Identity = 206/272 (75.74%), Postives = 237/272 (87.13%), Query Frame = 1

Query: 5   VAMQKALLLTAVALIGIIAGAA-AQNCGCAAGLCCSRFGFCGTGDDFCGSGCTEGPCNIP 64
           +A +  +L   +   G+++G A AQNCGCAA LCCS++G+CGT D +CG GC  GPC  P
Sbjct: 1   MAARSIILTLFIVATGVLSGYADAQNCGCAADLCCSQYGYCGTDDAYCGKGCQSGPCKTP 60

Query: 65  PLTPSVNDVVVADFVTEEFFNGIINQADAGCAGRGFYSRATFLEALRSFDRFGRIGSVDD 124
             TPS NDVVVAD VT EFFNGII+QADA CAG+ FYSRATFLEAL S+++FGRIGSVDD
Sbjct: 61  AQTPSTNDVVVADIVTPEFFNGIIDQADASCAGKNFYSRATFLEALNSYNQFGRIGSVDD 120

Query: 125 SKREIAAFFAHVTHETGHLCFIEEIDGATKDYCDEENTQYPCNPSKGYFGRGPIQLSWNF 184
           SKREIAAFFAHVTHETGH C+IEEIDGA+KDYCDE NTQYPCNP+KGY+GRGPIQLSWNF
Sbjct: 121 SKREIAAFFAHVTHETGHFCYIEEIDGASKDYCDENNTQYPCNPNKGYYGRGPIQLSWNF 180

Query: 185 NYGPAGENIGFDGLNNPEIVATDPVVSFKTALWYWMNFVRPVINQGFGATIRAINGALEC 244
           NYGPAGE+IGFDGLN+PE VA DPV++FKTALWYWMN VRPVI +GFGATIRAINGALEC
Sbjct: 181 NYGPAGESIGFDGLNSPETVANDPVIAFKTALWYWMNNVRPVIGEGFGATIRAINGALEC 240

Query: 245 DGANNATVQRRVQFYTQYCDQLGVAPGNNLTC 276
           DG N ATVQ+RV++YT+YC+QLGVAPG+NLTC
Sbjct: 241 DGGNPATVQKRVEYYTEYCNQLGVAPGDNLTC 272

BLAST of Lsi02G009130 vs. NCBI nr
Match: gi|702236384|ref|XP_010055735.1| (PREDICTED: endochitinase PR4-like [Eucalyptus grandis])

HSP 1 Score: 450.7 bits (1158), Expect = 1.9e-123
Identity = 210/272 (77.21%), Postives = 236/272 (86.76%), Query Frame = 1

Query: 9   KALLLTAVALIGIIAGA-----AAQNCGCAAGLCCSRFGFCGTGDDFCGSGCTEGPCNIP 68
           + LLLTAVA+ GII  A     +AQNCGCAA  CCSR+G+CGTGDD+CG+GC EGPCN P
Sbjct: 7   RKLLLTAVAVAGIIISALPDTTSAQNCGCAADQCCSRWGYCGTGDDYCGTGCQEGPCNAP 66

Query: 69  PLTPSVNDVVVADFVTEEFFNGIINQADAGCAGRGFYSRATFLEALRSFDRFGRIGSVDD 128
           P T   NDVVVAD VTE FF+GII QA   C G+GFYSR TFLEA  S+ +FGR+GSVDD
Sbjct: 67  PPT---NDVVVADVVTEAFFDGIIGQAAESCEGKGFYSRGTFLEAAGSYPQFGRVGSVDD 126

Query: 129 SKREIAAFFAHVTHETGHLCFIEEIDGATKDYCDEENTQYPCNPSKGYFGRGPIQLSWNF 188
           SKREIAAFFAHVTHETGH C+I EIDGA+KDYCDE NTQYPC+P+KGY+GRGPIQLSWNF
Sbjct: 127 SKREIAAFFAHVTHETGHFCYINEIDGASKDYCDETNTQYPCSPNKGYYGRGPIQLSWNF 186

Query: 189 NYGPAGENIGFDGLNNPEIVATDPVVSFKTALWYWMNFVRPVINQGFGATIRAINGALEC 248
           NYGPAGE+IGFDGLN+P+ VATDP++SFKTALWYWMNFVRPVI+QGFGATIRAINGALEC
Sbjct: 187 NYGPAGESIGFDGLNSPDTVATDPLISFKTALWYWMNFVRPVIDQGFGATIRAINGALEC 246

Query: 249 DGANNATVQRRVQFYTQYCDQLGVAPGNNLTC 276
           DG N ATVQ RVQ+YT+YC QLGVAPG+NLTC
Sbjct: 247 DGGNPATVQARVQYYTEYCSQLGVAPGDNLTC 275

BLAST of Lsi02G009130 vs. NCBI nr
Match: gi|657955457|ref|XP_008369197.1| (PREDICTED: endochitinase PR4-like [Malus domestica])

HSP 1 Score: 448.7 bits (1153), Expect = 7.1e-123
Identity = 204/272 (75.00%), Postives = 236/272 (86.76%), Query Frame = 1

Query: 5   VAMQKALLLTAVALIGIIAGAA-AQNCGCAAGLCCSRFGFCGTGDDFCGSGCTEGPCNIP 64
           +A +  +L   +   G+++G A AQNCGCAA  CCS++G+CGT D +CG+GC  GPC  P
Sbjct: 1   MAARSIILTLFIVATGVLSGYADAQNCGCAADQCCSQYGYCGTDDAYCGTGCKSGPCKTP 60

Query: 65  PLTPSVNDVVVADFVTEEFFNGIINQADAGCAGRGFYSRATFLEALRSFDRFGRIGSVDD 124
             TPS +DVVVAD VT EFFNGII+QADA CAG+ FYSRATFLEAL S+ +FGRIGS+DD
Sbjct: 61  AQTPSTSDVVVADIVTPEFFNGIIDQADASCAGKNFYSRATFLEALNSYTQFGRIGSIDD 120

Query: 125 SKREIAAFFAHVTHETGHLCFIEEIDGATKDYCDEENTQYPCNPSKGYFGRGPIQLSWNF 184
           SKREIAAFFAHVTHETGH C+IEEIDGA+KDYCDE NTQYPCNP+KGY+GRGPIQLSWNF
Sbjct: 121 SKREIAAFFAHVTHETGHFCYIEEIDGASKDYCDETNTQYPCNPNKGYYGRGPIQLSWNF 180

Query: 185 NYGPAGENIGFDGLNNPEIVATDPVVSFKTALWYWMNFVRPVINQGFGATIRAINGALEC 244
           NYGPAGE+IGFDGLN+PE VA DPV++FKTALWYWMN VRPVI +GFGATIRAINGALEC
Sbjct: 181 NYGPAGESIGFDGLNSPETVANDPVIAFKTALWYWMNNVRPVIGEGFGATIRAINGALEC 240

Query: 245 DGANNATVQRRVQFYTQYCDQLGVAPGNNLTC 276
           DG N ATVQRRV++YT+YC+QLGVAPG+NLTC
Sbjct: 241 DGGNPATVQRRVEYYTEYCNQLGVAPGDNLTC 272

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
CHI5_ARATH1.2e-11475.90Endochitinase EP3 OS=Arabidopsis thaliana GN=EP3 PE=1 SV=1[more]
CHI4_PHAVU6.5e-10869.92Endochitinase PR4 OS=Phaseolus vulgaris GN=CHI4 PE=2 SV=1[more]
CHI6_ORYSJ4.3e-9659.64Chitinase 6 OS=Oryza sativa subsp. japonica GN=Cht6 PE=2 SV=1[more]
CHI4_ORYSJ5.9e-9357.19Chitinase 4 OS=Oryza sativa subsp. japonica GN=Cht4 PE=2 SV=2[more]
CHI5_ORYSJ5.0e-9257.45Chitinase 5 OS=Oryza sativa subsp. japonica GN=Cht5 PE=2 SV=1[more]
Match NameE-valueIdentityDescription
A0A0A0LXT8_CUCSA7.3e-15193.36Uncharacterized protein OS=Cucumis sativus GN=Csa_1G534750 PE=3 SV=1[more]
B9VQ34_PYRPY2.6e-12475.74Class IV chitinase OS=Pyrus pyrifolia PE=2 SV=1[more]
A0A077GZM8_9ROSA2.6e-12475.74Chitinase ppl-2 OS=Pyrus x bretschneideri PE=2 SV=1[more]
A0A059DAK6_EUCGR1.3e-12377.21Uncharacterized protein OS=Eucalyptus grandis GN=EUGRSUZ_A00020 PE=3 SV=1[more]
A0A061ER07_THECC3.2e-12276.69Carrot EP3-3 chitinase OS=Theobroma cacao GN=TCM_019914 PE=3 SV=1[more]
Match NameE-valueIdentityDescription
AT3G54420.16.9e-11675.90 homolog of carrot EP3-3 chitinase[more]
AT2G43590.18.7e-8755.88 Chitinase family protein[more]
AT2G43580.13.8e-8251.84 Chitinase family protein[more]
AT2G43570.14.4e-7050.58 chitinase, putative[more]
AT2G43610.12.9e-6645.39 Chitinase family protein[more]
Match NameE-valueIdentityDescription
gi|659068474|ref|XP_008444611.1|6.2e-15193.09PREDICTED: endochitinase PR4-like [Cucumis melo][more]
gi|449454971|ref|XP_004145227.1|1.1e-15093.36PREDICTED: endochitinase PR4-like [Cucumis sativus][more]
gi|222139394|gb|ACM45716.1|3.8e-12475.74class IV chitinase [Pyrus x bretschneideri x Pyrus pyrifolia][more]
gi|702236384|ref|XP_010055735.1|1.9e-12377.21PREDICTED: endochitinase PR4-like [Eucalyptus grandis][more]
gi|657955457|ref|XP_008369197.1|7.1e-12375.00PREDICTED: endochitinase PR4-like [Malus domestica][more]
The following terms have been associated with this gene:
Vocabulary: Biological Process
TermDefinition
GO:0005975carbohydrate metabolic process
GO:0016998cell wall macromolecule catabolic process
GO:0006032chitin catabolic process
Vocabulary: Molecular Function
TermDefinition
GO:0008061chitin binding
GO:0004568chitinase activity
Vocabulary: INTERPRO
TermDefinition
IPR023346Lysozyme-like_dom_sf
IPR018371Chitin-binding_1_CS
IPR016283Glyco_hydro_19
IPR001002Chitin-bd_1
IPR000726Glyco_hydro_19_cat
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0005975 carbohydrate metabolic process
biological_process GO:0016998 cell wall macromolecule catabolic process
biological_process GO:0006032 chitin catabolic process
biological_process GO:0009617 response to bacterium
biological_process GO:0009611 response to wounding
biological_process GO:0010262 somatic embryogenesis
cellular_component GO:0005575 cellular_component
cellular_component GO:0005618 cell wall
molecular_function GO:0004568 chitinase activity
molecular_function GO:0008061 chitin binding

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Lsi02G009130.1Lsi02G009130.1mRNA


Analysis Name: InterPro Annotations of Lagenaria siceraria
Date Performed: 2017-09-18
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR000726Glycoside hydrolase, family 19, catalyticPFAMPF00182Glyco_hydro_19coord: 78..275
score: 1.6
IPR000726Glycoside hydrolase, family 19, catalyticPROSITEPS00773CHITINASE_19_1coord: 94..116
scor
IPR000726Glycoside hydrolase, family 19, catalyticPROSITEPS00774CHITINASE_19_2coord: 209..219
scor
IPR001002Chitin-binding, type 1GENE3DG3DSA:3.30.60.10coord: 24..67
score: 1.5
IPR001002Chitin-binding, type 1PFAMPF00187Chitin_bind_1coord: 24..60
score: 5.
IPR001002Chitin-binding, type 1SMARTSM00270ChitinBD_3coord: 29..60
score: 2.
IPR001002Chitin-binding, type 1PROFILEPS50941CHIT_BIND_I_2coord: 27..62
score: 9
IPR001002Chitin-binding, type 1unknownSSF57016Plant lectins/antimicrobial peptidescoord: 23..65
score: 7.07
IPR016283Glycoside hydrolase, family 19PIRPIRSF001060Endochitinasecoord: 3..275
score: 8.7E
IPR018371Chitin-binding, type 1, conserved sitePROSITEPS00026CHIT_BIND_I_1coord: 32..51
scor
IPR023346Lysozyme-like domainunknownSSF53955Lysozyme-likecoord: 74..275
score: 3.37
NoneNo IPR availableGENE3DG3DSA:1.10.530.10coord: 204..275
score: 3.9E-54coord: 76..141
score: 3.9
NoneNo IPR availableGENE3DG3DSA:3.30.20.10coord: 152..203
score: 6.3
NoneNo IPR availablePANTHERPTHR22595CHITINASE-RELATEDcoord: 4..275
score: 9.2E
NoneNo IPR availablePANTHERPTHR22595:SF61SUBFAMILY NOT NAMEDcoord: 4..275
score: 9.2E

The following gene(s) are paralogous to this gene:

None