Lsi01G020040 (gene) Bottle gourd (USVL1VR-Ls) | Cucurbit Genomics Database (CuGenDB)

Overview


Name	Lsi01G020040
Type	gene
Organism	Lagenaria siceraria (Bottle gourd (USVL1VR-Ls))
Description	Rhomboid family protein
Location	chr01 : 26962587 .. 26966278 (-)

Genome Viewer

Gene Expression

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: five_prime_UTRCDSthree_prime_UTR

Hold the cursor over a type above to highlight its positions in the sequence below.

CGTAGGTGGCAATCCAAGGCGGAGTTATTTTGAACTTCGAAACCCTTTCCCTCAAATACTCAACTTTGGTGCTTCATTCATTCTTTTTTGCTCTTTCAATTTCAACTGGTAAGATTCACAGTTTATTTCTCAATTTCTAGCTTTCAAACCCCAATTCTCTGATTTCTTCACTCGTCGATTTATTCATTTTTTGCAAAATATGGACTATCTTTTGCTCGAATCACGCTTGTTTAATCAACGTGGTGAATTTCTTTCTTCCTTTGTCGCATGTTCTTGGTTTCGTAATTTTACAATAGGAACAGTTGATGCTCTCTCGTTGTCCAAAATTATTGTTTGTTTGTAGTGTAGAGTTAGAATACGAGAGTATCTTTTCTCGTAGGTGTTGTTTTGAACTTTTGACATTTAATGATTGTGAGCTATGTGAATTTCCATACATGAGTTATTTTGTTTAAGTTATATGGACTCCTTTAAAATTAATTTTCATTTGGATTTGGAACGGTCTTGGAATTTGAGGATGTAATACTGCTAATTGCATTTCCAGTTATCCTGGAAATGTATAAATGTGAATAAAACGAGTTGTGGAAAAAGAAAGCATCAAAACTATCGATCAAAACAATCAAGAGTGGTTTAAAAGGTTAAATATCAATTAAGGATTTAGAATGTCAGGTTTCAAACCAAATATTTTGTGTATGCATAACCTTCTTGGCCTTTCCCTGTACATAATATAAAATCACAACCAATAAAAGGGCACATGCCAAAATCGACCTAATGACAAATCAGAGGTTGGAGAAACATATTTTTAACAACCCAGTGGGTGCAAAGCTAACAGTCATCTTCAGCTATGCGTAACTTGAGGAGTTGCTATGGCAACTCAGAAATGCATTGCTAAAAGCTTCTCTAGTGATGTTGGACTTTTTGTGACGACTCTTTGGTGTCACGGAATATATAACATTTTGTAATGTACTTTAGATGGATATCTTATGGTTGCAGACTTCTCTATGGCCATGAATAGAGGCTGTCAAATTATATATCCACCACTGAACAGCCTAATTTGCATCAGTTCACCAGGCCTTCTGTTGTGAAATTCTTTTATCATTTTATCTATAATTGTGGTAGCCATTCACTCGAAACTGTGAGACTTGGAGGGCCTCTTTACTCCACCCATTTCCCCCCACTTGAACCTTGATTATAATTTGGTACTGATTGCTTCCTGAAAGAGGTACTATGCTAATTTAAGAACAAGGGCTTGCTCCAGGCAGTTTGTACCCTAGTTATATATGTATTTCTTTCTGAAACAATATTTGCTTGAGTTGCAATGCATTAATTGCTTGTGACTTGTGCTGTTTTATCTAACTAGCTTTAGAGAATGCTGCGATTTCCTTTTTGTATAATTGTCCCTACTTTAACCATCTTGTTATTGGATACTACAGGAAATAAGCTCACGACAGAATGCTGCCACTACTAGCTTACCAAGTGATTAGCCAATATTACAGGCTCCAAAGGAACCCCATCACTGTGGGGCTCCTTGCCACTAATACTCTCATTCATCTAAGGCCCAATTTTCTTCATCCTATCATCCCTTCCATAGAGGATGTCTGGTTTAATGCGCATCTCATCCTCAAGGTGACTATTCCAGCTAATCATCTAATTCTTTGGTCCTGCTCCCTGTTTAAGACTAGGTAGCATTGTTTGTTTTATTTTTTAGTGCACTCTCTTTCTTAGGGTTAATTGGTGATAATTATAAGAATTGAATGATAGATCTTTCTGTTTAGGATCTTCCTATTTATTAACACATTATTAGGAAGCTCCTGAACAGAATGGCATGTTGTTTCCATGGGATTTAATGCGTTACTATCTCATTCAGAAGATTTCATATTCCTTTTTCGATCATCTATATAATAAATTTTGTAGTTTCCTTTGATACTGACTACCTGTTTCCTAGCATCATTATAACAAATAGGGTTTGTTTATATATATATATATATAGTTGGAAATTTTTCTTTCATTGAGAACAATGGAAGAAATCAGTGGATTCTATTTGAATTTGTATGCTTTCACTATAAGAAACGGTTCATGGTATGATGTTGAACATTACAAGATGCATCTAGATTGGTTTGCCCCCACATGACTGTGTAATTTGTGTCCTTACGAGTTGTCCTCAGAATGAGGTTATTGTATGATAGGCCTCGAATTATAGCTAACTCTGATTTTGAGAAAAATGAGAAGCTTTTAAAAGTTTTGATGCTTTGATTGTTTTACAATATCGTCAGGTTCTTGCTTTTTTAGTAGTGTATTCCTTTAATCTCCCTTGATAAGGATATACCTAGGAATTCAGAGCTCTTGTAGGATTATTTTCTTTTTGGTTATTTATCAATTTGTTTGTATGTCTTTTTTGAATGAGATAATGTGATATTGGCATTACAACACTGTCATAATATTGGTATCATTTGCATTTGCCAGCACAAGGAGTTGAAGCGTTTTCTACTATCACCATTCTACCATGTGGGTGACCATCACCTTGTCTACAATATGATATCTTTACTATGGAAAGGCATTCAATTGGAAACATCAATGGGTAGTATCGAATTCGCTTCAATGGTTGGTGCATTACTCTTAATGTCGCAGGGAATTACCTTGTTGCTCATTAAATCCCTACTGGTTTTCTTTAATTATGAGAGGTCTTACTTCTATGAATATTCTGTTGGGTTTTCCGGTGTACTTTTTGCTATGAAAGTTGTTCTTGGTTCTCAAGCTGAAAGCTACACTTACCTTCATGGTCTAAGAGTACCATCAAGTCATGCTGCATGGTTAGAACTAATACTTATCCAAATGATTTCACCTGGCGTCTCTTTCCTTGGACATCTTGGTGGTATACTTGCTGGTCTTCTCTTTTTGCATCTGAAGGGTACGAATTTAGGGTTGGATCCGTTGACTGTTCTTATTAGAAGTGTTGGTCGAATATTGAGTCGTCCTCTTGGATTTTTCTGTCGCTTGAATCCATTTAGGCAGCGTACGATATTTGGAGGAGGAACTGTCTGTAGACGACAGACAGCGGCCTTGGAAAGGACTACGAGTATCGATACGTGGAGGTGTCAAGCATGTACATTTGACAATTCTTGTGTATTAAGCATATGTGAAATGTGTGGGACAGATCGGCAGGATAGGCTACTGTCTCCTCATCAATTATCACATCATTTTGGTTTAACTCGAGAGGAAGTGCGTCGTCGGAGGCTTGAAAGATTTGGATGATGATAGAATAGATTGTTCTTTTTTCTCAGGCTACCAGGTATTTGCTTTTAGATGAGTAAATTGAAAGGCTAGCACTAGATATCTTGTTAATGAAGTTGTTTGCTTGATGTTAAAAAATAAAGTGATATGGAGCATGGAGCATGTGTTTAGGAACTAAGCAAATTTGATTGAATACAGATTGGAGAATCTTGTTCATAGGGGAGGAATTAATATAGTTTCTTTTCATCATTGTTTTTCTATATTACATTTGTATGCTTCTTTGTAATGGTTGCAATGACCCAACTTTTCTAAGATATCTCATTGGAGAAATTATTACATATATGACATTCATGATTGAAAACTTGGGTTTTTTTAATTAAAAAAGAAAAAAGAAAAAAATTGAACTGAACTGAACTTTATTTAAAAGGCGAGTAAAGGAAAATGTGCATGAACCTCATTAACAC

mRNA sequence

CGTAGGTGGCAATCCAAGGCGGAGTTATTTTGAACTTCGAAACCCTTTCCCTCAAATACTCAACTTTGGTGCTTCATTCATTCTTTTTTGCTCTTTCAATTTCAACTGGAAATAAGCTCACGACAGAATGCTGCCACTACTAGCTTACCAAGTGATTAGCCAATATTACAGGCTCCAAAGGAACCCCATCACTGTGGGGCTCCTTGCCACTAATACTCTCATTCATCTAAGGCCCAATTTTCTTCATCCTATCATCCCTTCCATAGAGGATGTCTGGTTTAATGCGCATCTCATCCTCAAGCACAAGGAGTTGAAGCGTTTTCTACTATCACCATTCTACCATGTGGGTGACCATCACCTTGTCTACAATATGATATCTTTACTATGGAAAGGCATTCAATTGGAAACATCAATGGGTAGTATCGAATTCGCTTCAATGGTTGGTGCATTACTCTTAATGTCGCAGGGAATTACCTTGTTGCTCATTAAATCCCTACTGGTTTTCTTTAATTATGAGAGGTCTTACTTCTATGAATATTCTGTTGGGTTTTCCGGTGTACTTTTTGCTATGAAAGTTGTTCTTGGTTCTCAAGCTGAAAGCTACACTTACCTTCATGGTCTAAGAGTACCATCAAGTCATGCTGCATGGTTAGAACTAATACTTATCCAAATGATTTCACCTGGCGTCTCTTTCCTTGGACATCTTGGTGGTATACTTGCTGGTCTTCTCTTTTTGCATCTGAAGGGTACGAATTTAGGGTTGGATCCGTTGACTGTTCTTATTAGAAGTGTTGGTCGAATATTGAGTCGTCCTCTTGGATTTTTCTGTCGCTTGAATCCATTTAGGCAGCGTACGATATTTGGAGGAGGAACTGTCTGTAGACGACAGACAGCGGCCTTGGAAAGGACTACGAGTATCGATACGTGGAGGTGTCAAGCATGTACATTTGACAATTCTTGTGTATTAAGCATATGTGAAATGTGTGGGACAGATCGGCAGGATAGGCTACTGTCTCCTCATCAATTATCACATCATTTTGGTTTAACTCGAGAGGAAGTGCGTCGTCGGAGGCTTGAAAGATTTGGATGATGATAGAATAGATTGTTCTTTTTTCTCAGGCTACCAGGTATTTGCTTTTAGATGAGTAAATTGAAAGGCTAGCACTAGATATCTTGTTAATGAAGTTGTTTGCTTGATGTTAAAAAATAAAGTGATATGGAGCATGGAGCATGTGTTTAGGAACTAAGCAAATTTGATTGAATACAGATTGGAGAATCTTGTTCATAGGGGAGGAATTAATATAGTTTCTTTTCATCATTGTTTTTCTATATTACATTTGTATGCTTCTTTGTAATGGTTGCAATGACCCAACTTTTCTAAGATATCTCATTGGAGAAATTATTACATATATGACATTCATGATTGAAAACTTGGGTTTTTTTAATTAAAAAAGAAAAAAGAAAAAAATTGAACTGAACTGAACTTTATTTAAAAGGCGAGTAAAGGAAAATGTGCATGAACCTCATTAACAC

Coding sequence (CDS)

ATGCTGCCACTACTAGCTTACCAAGTGATTAGCCAATATTACAGGCTCCAAAGGAACCCCATCACTGTGGGGCTCCTTGCCACTAATACTCTCATTCATCTAAGGCCCAATTTTCTTCATCCTATCATCCCTTCCATAGAGGATGTCTGGTTTAATGCGCATCTCATCCTCAAGCACAAGGAGTTGAAGCGTTTTCTACTATCACCATTCTACCATGTGGGTGACCATCACCTTGTCTACAATATGATATCTTTACTATGGAAAGGCATTCAATTGGAAACATCAATGGGTAGTATCGAATTCGCTTCAATGGTTGGTGCATTACTCTTAATGTCGCAGGGAATTACCTTGTTGCTCATTAAATCCCTACTGGTTTTCTTTAATTATGAGAGGTCTTACTTCTATGAATATTCTGTTGGGTTTTCCGGTGTACTTTTTGCTATGAAAGTTGTTCTTGGTTCTCAAGCTGAAAGCTACACTTACCTTCATGGTCTAAGAGTACCATCAAGTCATGCTGCATGGTTAGAACTAATACTTATCCAAATGATTTCACCTGGCGTCTCTTTCCTTGGACATCTTGGTGGTATACTTGCTGGTCTTCTCTTTTTGCATCTGAAGGGTACGAATTTAGGGTTGGATCCGTTGACTGTTCTTATTAGAAGTGTTGGTCGAATATTGAGTCGTCCTCTTGGATTTTTCTGTCGCTTGAATCCATTTAGGCAGCGTACGATATTTGGAGGAGGAACTGTCTGTAGACGACAGACAGCGGCCTTGGAAAGGACTACGAGTATCGATACGTGGAGGTGTCAAGCATGTACATTTGACAATTCTTGTGTATTAAGCATATGTGAAATGTGTGGGACAGATCGGCAGGATAGGCTACTGTCTCCTCATCAATTATCACATCATTTTGGTTTAACTCGAGAGGAAGTGCGTCGTCGGAGGCTTGAAAGATTTGGATGA

Protein sequence

MLPLLAYQVISQYYRLQRNPITVGLLATNTLIHLRPNFLHPIIPSIEDVWFNAHLILKHKELKRFLLSPFYHVGDHHLVYNMISLLWKGIQLETSMGSIEFASMVGALLLMSQGITLLLIKSLLVFFNYERSYFYEYSVGFSGVLFAMKVVLGSQAESYTYLHGLRVPSSHAAWLELILIQMISPGVSFLGHLGGILAGLLFLHLKGTNLGLDPLTVLIRSVGRILSRPLGFFCRLNPFRQRTIFGGGTVCRRQTAALERTTSIDTWRCQACTFDNSCVLSICEMCGTDRQDRLLSPHQLSHHFGLTREEVRRRRLERFG

Homology

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BLAST of Lsi01G020040 vs. Swiss-Prot
Match: RBL14_ARATH (Rhomboid-like protein 14, mitochondrial OS=Arabidopsis thaliana GN=RBL14 PE=2 SV=1)

HSP 1 Score: 347.1 bits (889), Expect = 2.1e-94
Identity = 186/324 (57.41%), Postives = 233/324 (71.91%), Query Frame = 1


Query: 1   MLPLLAYQVISQYYRLQ-RNPITVGLLATNTLIHLRPNFLHPIIPSIEDVWFNAHLILKH 60
           MLPLLA   +++YYRL  + P+T  LLA NTL++LRP F+ P+IP I +VWFN HLI KH
Sbjct: 14  MLPLLALSAVAEYYRLPWKPPVTASLLAANTLVYLRPAFIDPVIPHISEVWFNPHLIFKH 73

Query: 61  KELKRFLLSPFYHVGDHHLVYNMISLLWKGIQLETSMGSIEFASMVGALLLMSQGITLLL 120
           K+LKR  LS FYHV + HLVYNM+SLLWKGI+LETSMGS EFASMV  L+ MSQG+TLLL
Sbjct: 74  KDLKRLFLSAFYHVNEPHLVYNMMSLLWKGIKLETSMGSSEFASMVFTLIGMSQGVTLLL 133

Query: 121 IKSLLVFFNYERSYFYEYSVGFSGVLFAMKVVLGSQAESYTYLHGLRVPSSHAAWLELIL 180
            KSLL+ F+Y+R+Y+ EY+VGFSGVLFAMKVVL SQAE Y+ ++G+ VP+ +AAW ELIL
Sbjct: 134 AKSLLLLFDYDRAYYNEYAVGFSGVLFAMKVVLNSQAEDYSSVYGILVPTKYAAWAELIL 193

Query: 181 IQMISPGVSFLGHLGGILAGLLFLHLKGTNLGLDPLTVLIRSVGRILSRPLGFFCRLNPF 240
           +QM  P  SFLGHLGGILAG+++L LKG+  G DP+T+ +R V R+++ PL F   +   
Sbjct: 194 VQMFVPNASFLGHLGGILAGIIYLKLKGSYSGSDPVTMAVRGVSRLVTWPLRFLNGMVRS 253

Query: 241 RQRTIFGGGTVCRRQTAALERTTSIDTWRCQACTFDNSCVLSICEMCGTDRQD----RLL 300
           R+R I G G V R QT           WRCQ+CT+DNS  LS CEMCG+ R       L 
Sbjct: 254 RRRRITGRGRVGRGQTG----IAGPGIWRCQSCTYDNSGWLSACEMCGSGRARGNGWSLN 313

Query: 301 SPHQLSHHFGLTREEVRRRRLERF 320
               LS    L  +E+RRRR+ERF
Sbjct: 314 QGPALSSSNDLPLDELRRRRVERF 333

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BLAST of Lsi01G020040 vs. Swiss-Prot
Match: RHBL4_HUMAN (Rhomboid-related protein 4 OS=Homo sapiens GN=RHBDD1 PE=1 SV=1)

HSP 1 Score: 115.5 bits (288), Expect = 1.0e-24
Identity = 102/323 (31.58%), Postives = 154/323 (47.68%), Query Frame = 1


Query: 9   VISQYYRLQRN---PITVGLLATNTLIHLRPNF-LHPIIPSIEDVWFNAHLILKHKELKR 68
           ++SQ + +  N   P+T+  LA N    L P   L+    S+E  +       + K+ +R
Sbjct: 15  LLSQIFHVGINNIPPVTLATLALNIWFFLNPQKPLYSSCLSVEKCY-------QQKDWQR 74

Query: 69  FLLSPFYHVGDHHLVYNMISLLWKGIQLETSMGSIEFASMVGALLLMSQGITLLLIKSLL 128
            LLSP +H  D HL +NM S+LWKGI LE  +GS  FA ++ A  +++ G+  LL++  +
Sbjct: 75  LLLSPLHHADDWHLYFNMASMLWKGINLERRLGSRWFAYVITAFSVLT-GVVYLLLQFAV 134

Query: 129 VFFNYERSYFYEYSVGFSGVLFAMKVVLGSQAE-SYTYLHGLRVPSSHAAWLELILIQMI 188
             F  E  +    +VGFSGVLFA+KV+        +  + G  VP+  A W+EL+ I + 
Sbjct: 135 AEFMDEPDFKRSCAVGFSGVLFALKVLNNHYCPGGFVNILGFPVPNRFACWVELVAIHLF 194

Query: 189 SPGVSFLGHLGGILAGLLFLHLKGTNLGLDPLTVLIRSVGRILSRPLGFFCRLNPFRQRT 248
           SPG SF GHL GIL GL++           PL  ++ +     S  +G+     P RQ  
Sbjct: 195 SPGTSFAGHLAGILVGLMYTQ--------GPLKKIMEACAGGFSSSVGY-----PGRQYY 254

Query: 249 IFGGGT-------VCRRQTAALERTTSIDTWRCQACTFDNSCVLSICEMCGTDRQDRLLS 308
               G+          R     E   + DT+   A   +   +    +    DR +   S
Sbjct: 255 FNSSGSSGYQDYYPHGRPDHYEEAPRNYDTY--TAGLSEEEQLERALQASLWDRGNTRNS 312

Query: 309 PHQLSHHFGLTREEVRRRRLERF 320
           P     H  L+ EE+RR+RL RF
Sbjct: 315 PPPYGFH--LSPEEMRRQRLHRF 312

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BLAST of Lsi01G020040 vs. Swiss-Prot
Match: RHBL4_MOUSE (Rhomboid-related protein 4 OS=Mus musculus GN=Rhbdd1 PE=1 SV=1)

HSP 1 Score: 111.7 bits (278), Expect = 1.5e-23
Identity = 71/199 (35.68%), Postives = 111/199 (55.78%), Query Frame = 1


Query: 9   VISQYYRLQRN---PITVGLLATNTLIHLRP-NFLHPIIPSIEDVWFNAHLILKHKELKR 68
           ++SQ +++  N   P+T+  LA N    L P   L+    S+E  +       + K+ +R
Sbjct: 15  LLSQVFQIGINNIPPVTLATLAVNVWFFLNPWKPLYHSCISVEKCY-------QQKDWQR 74

Query: 69  FLLSPFYHVGDHHLVYNMISLLWKGIQLETSMGSIEFASMVGALLLMSQGITLLLIKSLL 128
            LLSP +H  D HL +NM+S+LWKG++LE  +GS  FA ++    L++ G+  LL++  +
Sbjct: 75  LLLSPLHHGDDWHLYFNMVSMLWKGVKLERRLGSRWFAYVIATFSLLT-GVVYLLLQFTV 134

Query: 129 VFFNYERSYFYEYSVGFSGVLFAMKVVLGSQAE-SYTYLHGLRVPSSHAAWLELILIQMI 188
                +  +    +VGFSGVLFA+KV+        +  + G  VP+  A W EL+ I   
Sbjct: 135 AELLNQPDFKRNCAVGFSGVLFALKVLSNHYCPGGFVNILGFPVPNRFACWAELVAIHFC 194

Query: 189 SPGVSFLGHLGGILAGLLF 203
           +PG SF GHL GIL GL++
Sbjct: 195 TPGTSFAGHLAGILVGLMY 205

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BLAST of Lsi01G020040 vs. Swiss-Prot
Match: RHBL4_PONAB (Rhomboid-related protein 4 OS=Pongo abelii GN=RHBDD1 PE=2 SV=1)

HSP 1 Score: 108.6 bits (270), Expect = 1.3e-22
Identity = 71/199 (35.68%), Postives = 109/199 (54.77%), Query Frame = 1


Query: 9   VISQYYRLQRN---PITVGLLATNTLIHLRPNF-LHPIIPSIEDVWFNAHLILKHKELKR 68
           ++SQ + +  N   P+T+  LA N    L P   L+    S+E  +       + ++ +R
Sbjct: 15  LLSQIFHVGINNIPPVTLATLALNIWFFLNPQKPLYSSCLSVEKCY-------QQRDWQR 74

Query: 69  FLLSPFYHVGDHHLVYNMISLLWKGIQLETSMGSIEFASMVGALLLMSQGITLLLIKSLL 128
            LLSP +H  D HL +N  S+LWKGI LE  +GS  FA ++    +++ G+  LL++  +
Sbjct: 75  LLLSPLHHADDWHLYFNTASVLWKGINLERRLGSRWFAYVITTFSVLT-GVVYLLLQFAV 134

Query: 129 VFFNYERSYFYEYSVGFSGVLFAMKVVLGSQAE-SYTYLHGLRVPSSHAAWLELILIQMI 188
             F  E  +    +VGFSGVLFA+KV+        +  + G  VP+  A W+EL+ I + 
Sbjct: 135 AEFMDEPDFKRSCAVGFSGVLFALKVLNNHYCPGGFVNILGFPVPNRFACWVELVAIHLF 194

Query: 189 SPGVSFLGHLGGILAGLLF 203
           SPG SF GH  GIL GL++
Sbjct: 195 SPGTSFAGHQAGILVGLMY 205

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BLAST of Lsi01G020040 vs. Swiss-Prot
Match: RHBL4_RAT (Rhomboid-related protein 4 OS=Rattus norvegicus GN=Rhbdd1 PE=2 SV=1)

HSP 1 Score: 106.7 bits (265), Expect = 4.8e-22
Identity = 70/199 (35.18%), Postives = 107/199 (53.77%), Query Frame = 1


Query: 9   VISQYYRLQRN---PITVGLLATNTLIHLRP-NFLHPIIPSIEDVWFNAHLILKHKELKR 68
           ++SQ + +  N   P+T+  LA N    L P   L+    S+E  +       +  + +R
Sbjct: 15  LLSQVFHIGINNIPPVTLATLAVNVWFFLNPWKPLYHSCISVEKCY-------QQNDWQR 74

Query: 69  FLLSPFYHVGDHHLVYNMISLLWKGIQLETSMGSIEFASMVGALLLMSQGITLLLIKSLL 128
            LLSP +H  D HL +NM+S+LWKG++LE  +GS  FA ++    L++ G+  LL++   
Sbjct: 75  LLLSPVHHGDDWHLYFNMVSMLWKGVKLEKRLGSRWFAYIIATFSLLT-GVVYLLLQFAS 134

Query: 129 VFFNYERSYFYEYSVGFSGVLFAMKVVLGSQAE-SYTYLHGLRVPSSHAAWLELILIQMI 188
                +  +    +VGFSGVLFA+KV+        +  + G  VP+  A W EL  I   
Sbjct: 135 AELMNQPDFKRNCAVGFSGVLFALKVLSNHYCPGGFVNILGFPVPNRFACWAELAAIHFC 194

Query: 189 SPGVSFLGHLGGILAGLLF 203
           +PG SF GHL GIL GL++
Sbjct: 195 TPGTSFAGHLAGILVGLMY 205

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BLAST of Lsi01G020040 vs. TrEMBL
Match: A0A0A0L173_CUCSA (Uncharacterized protein OS=Cucumis sativus GN=Csa_4G506850 PE=4 SV=1)

HSP 1 Score: 522.7 bits (1345), Expect = 3.1e-145
Identity = 267/320 (83.44%), Postives = 285/320 (89.06%), Query Frame = 1


Query: 1   MLPLLAYQVISQYYRLQRNPITVGLLATNTLIHLRPNFLHPIIPSIEDVWFNAHLILKHK 60
           MLPLLAYQV+SQYYRLQ NPIT+GLLA+NTLIHLRPNFLH IIPSI++VWFNAHLI KHK
Sbjct: 1   MLPLLAYQVVSQYYRLQGNPITLGLLASNTLIHLRPNFLHHIIPSIDEVWFNAHLIFKHK 60

Query: 61  ELKRFLLSPFYHVGDHHLVYNMISLLWKGIQLETSMGSIEFASMVGALLLMSQGITLLLI 120
           +LKRFLLSPFYHVGD HLVYNMISLLWKG QLETSMGSIEFASMVGALL+MSQGITLLLI
Sbjct: 61  DLKRFLLSPFYHVGDPHLVYNMISLLWKGTQLETSMGSIEFASMVGALLIMSQGITLLLI 120

Query: 121 KSLLVFFNYERSYFYEYSVGFSGVLFAMKVVLGSQAESYTYLHGLRVPSSHAAWLELILI 180
           KSLL+FFNY R Y+YEYSVGFSGVLFAMKVVLGS+ +SYTY+HGLRVPSSHAAWLELILI
Sbjct: 121 KSLLIFFNYGRPYYYEYSVGFSGVLFAMKVVLGSETDSYTYVHGLRVPSSHAAWLELILI 180

Query: 181 QMISPGVSFLGHLGGILAGLLFLHLKGTNLGLDPLTVLIRSVGRILSRPLGFFCRLNPFR 240
           QM+SPGVSFLGHLGGILAGLLFL LKGTN G DPLT+LIRSVGRILSR  GFFCRLNPFR
Sbjct: 181 QMVSPGVSFLGHLGGILAGLLFLRLKGTNSGSDPLTLLIRSVGRILSRSYGFFCRLNPFR 240

Query: 241 QRTIFGGGTVCRRQTAALERTTSIDTWRCQACTFDNSCVLSICEMCGTDRQDRLLSPHQL 300
           QR IFGGGTV  R        TSI TWRCQACTFDNS +LS CEMCGTDRQD +L  +QL
Sbjct: 241 QRRIFGGGTVGSRP------VTSIGTWRCQACTFDNSGLLSACEMCGTDRQDGVLHSNQL 300

Query: 301 SHHFGLTREEVRRRRLERFG 321
           S    LT EE+RRRRLERFG
Sbjct: 301 SSQLDLTWEEMRRRRLERFG 314

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BLAST of Lsi01G020040 vs. TrEMBL
Match: A0A067JVV7_JATCU (Uncharacterized protein OS=Jatropha curcas GN=JCGZ_19107 PE=4 SV=1)

HSP 1 Score: 404.8 bits (1039), Expect = 9.6e-110
Identity = 214/322 (66.46%), Postives = 256/322 (79.50%), Query Frame = 1


Query: 1   MLPLLAYQVISQYYRLQ-RNPITVGLLATNTLIHLRPNFLHPIIPSIEDVWFNAHLILKH 60
           +LPLLA   +S+YYRL  + P+T GLLATNTLI+LRP+FLH I+PSI +VWFN++LILKH
Sbjct: 8   LLPLLAVHAVSEYYRLPWKPPVTAGLLATNTLIYLRPSFLHHILPSIHEVWFNSYLILKH 67

Query: 61  KELKRFLLSPFYHVGDHHLVYNMISLLWKGIQLETSMGSIEFASMVGALLLMSQGITLLL 120
           ++LKRF LSPFYHVG+ HLVYNMISLLWKGIQLE+SMGS EFASMV ALL MSQGITLLL
Sbjct: 68  RDLKRFFLSPFYHVGESHLVYNMISLLWKGIQLESSMGSAEFASMVAALLTMSQGITLLL 127

Query: 121 IKSLLVFFNYERSYFYEYSVGFSGVLFAMKVVLGSQAESYTYLHGLRVPSSHAAWLELIL 180
            KSLL+FF+Y R ++ EY+VGFSGVLFAMKVVL SQ+E YTY+HGL VP+ HAAW ELIL
Sbjct: 128 SKSLLLFFDYGRPFYSEYAVGFSGVLFAMKVVLNSQSEDYTYVHGLVVPARHAAWAELIL 187

Query: 181 IQMISPGVSFLGHLGGILAGLLFLHLKGTNLGLDPLTVLIRSVGRILSRPLGFFCRLNPF 240
           +QM  PGVSFLGHLGGILAG+L+L L+G +LG +PLT++I+++  IL+RPL F   L  F
Sbjct: 188 VQMFVPGVSFLGHLGGILAGILYLKLRGMHLGPNPLTLIIKNLAGILARPLRFLRGLFQF 247

Query: 241 RQRTIFGGGTVCRRQTAALERTTSIDTWRCQACTFDNSCVLSICEMCGTDRQDRLLSPHQ 300
           R++ I G GTV   Q     R+ S   WRCQACT+DNS  LS+CEMCGT R D   S  Q
Sbjct: 248 RRQQISGRGTVGGSQAG---RSVS-RVWRCQACTYDNSGWLSVCEMCGTTRGDNGSSSRQ 307

Query: 301 LSHHF-GLTREEVRRRRLERFG 321
            SH F   T EEVRRRR++RFG
Sbjct: 308 FSHQFDNPTMEEVRRRRIQRFG 325

[Close]

BLAST of Lsi01G020040 vs. TrEMBL
Match: A0A0A7E6S4_9AQUA (Rhomboid protein Ilepu_RBL14 OS=Ilex purpurea PE=2 SV=1)

HSP 1 Score: 380.6 bits (976), Expect = 1.9e-102
Identity = 203/322 (63.04%), Postives = 248/322 (77.02%), Query Frame = 1


Query: 1   MLPLLAYQVISQYYRLQRNP-ITVGLLATNTLIHLRPNFLHPIIPSIEDVWFNAHLILKH 60
           MLPLLA   +S+Y RL R P +T GLLA NTLI+LRP+FL  I+P++++VWFN HLILKH
Sbjct: 13  MLPLLALHAVSEYCRLDRKPPVTAGLLAANTLIYLRPSFLESILPTLDEVWFNPHLILKH 72

Query: 61  KELKRFLLSPFYHVGDHHLVYNMISLLWKGIQLETSMGSIEFASMVGALLLMSQGITLLL 120
           ++LKRF LS FYH+G+ HLVYNM+SLLWKGIQLETSMGS++FASMV ALL MSQGITLLL
Sbjct: 73  RDLKRFFLSAFYHMGESHLVYNMMSLLWKGIQLETSMGSVKFASMVAALLGMSQGITLLL 132

Query: 121 IKSLLVFFNYERSYFYEYSVGFSGVLFAMKVVLGSQAESYTYLHGLRVPSSHAAWLELIL 180
            KSLL+ F++ER+Y++EYSVGFSGVLFAMKV+L SQ++ YT +HGL +PS HAAW ELIL
Sbjct: 133 AKSLLLLFDHERAYYHEYSVGFSGVLFAMKVILNSQSDDYTSVHGLVLPSRHAAWAELIL 192

Query: 181 IQMISPGVSFLGHLGGILAGLLFLHLKGTNLGLDPLTVLIRSVGRILSRPLGFFCRLNPF 240
           IQM  PGVSFLGHLGGILAG+L+LHLK +  G +PLT +I    RILS P+     +  F
Sbjct: 193 IQMFVPGVSFLGHLGGILAGILYLHLKASYSGSNPLTTIIGRFARILSWPVRLVWGICRF 252

Query: 241 RQRTIFGGGTVCRRQTAALERTTSIDTWRCQACTFDNSCVLSICEMCGTDR-QDRLLSPH 300
           +Q  IFG GTV   QT +   TT +  WRCQACTFDNS  L++CEMCGT R  D L S  
Sbjct: 253 QQPRIFGRGTVGGSQTRS---TTGV--WRCQACTFDNSGWLNVCEMCGTGRFDDGLSSLG 312

Query: 301 QLSHHFGLTREEVRRRRLERFG 321
             +    L  EE+R+RR++RFG
Sbjct: 313 SSNQTHDLPVEELRQRRVQRFG 329

[Close]

BLAST of Lsi01G020040 vs. TrEMBL
Match: B9HXC8_POPTR (Rhomboid family protein OS=Populus trichocarpa GN=POPTR_0010s00720g PE=4 SV=1)

HSP 1 Score: 380.6 bits (976), Expect = 1.9e-102
Identity = 204/322 (63.35%), Postives = 246/322 (76.40%), Query Frame = 1


Query: 1   MLPLLAYQVISQYYRLQ-RNPITVGLLATNTLIHLRPNFLHPIIPSIEDVWFNAHLILKH 60
           MLPLLA   +S+YYRL  + P+T  LL  NTLI+LRP FL  I+PSI+ VWFN HLILK+
Sbjct: 11  MLPLLAIHAVSEYYRLPWKPPVTAALLGANTLIYLRPAFLRHILPSIDQVWFNPHLILKY 70

Query: 61  KELKRFLLSPFYHVGDHHLVYNMISLLWKGIQLETSMGSIEFASMVGALLLMSQGITLLL 120
           ++LKRFLLSPFYHV D HLVYNM+SLLWKGIQLETS+GS+EFASM+ ALL MSQGITLLL
Sbjct: 71  RDLKRFLLSPFYHVSDSHLVYNMMSLLWKGIQLETSVGSVEFASMLAALLAMSQGITLLL 130

Query: 121 IKSLLVFFNYERSYFYEYSVGFSGVLFAMKVVLGSQAESYTYLHGLRVPSSHAAWLELIL 180
            +SLL+FF+Y++ ++ EYS GFSGVLFAMKVVL SQ+ES T ++GL +P+ HAAW ELIL
Sbjct: 131 ARSLLLFFDYDKPFYSEYSAGFSGVLFAMKVVLNSQSESLTNVYGLVIPARHAAWAELIL 190

Query: 181 IQMISPGVSFLGHLGGILAGLLFLHLKGTNLGLDPLTVLIRSVGRILSRPLGFFCRLNPF 240
           IQM  PGVSFLGHLGGILAG+L++ LK    G DPLT+ IR++  ++S PL F   L  F
Sbjct: 191 IQMFVPGVSFLGHLGGILAGILYVKLKRAYSGPDPLTLTIRTLTNVISWPLRFVRNLFQF 250

Query: 241 RQRTIFGGGTVCRRQTAALERTTSIDTWRCQACTFDNSCVLSICEMCGTDRQDRLLSPHQ 300
           R+  I G G+V  RQT    R  S   WRCQACT+DNS +LS+CEMCGT R  R LS  +
Sbjct: 251 RRGRISGRGSVGGRQTG---RAMS-GLWRCQACTYDNSSLLSVCEMCGTSRGARGLSSRE 310

Query: 301 LSHHF-GLTREEVRRRRLERFG 321
            S H   LT EE+R RR+ERFG
Sbjct: 311 FSRHSDDLTLEEIRLRRIERFG 328

[Close]

BLAST of Lsi01G020040 vs. TrEMBL
Match: A5BGK5_VITVI (Putative uncharacterized protein OS=Vitis vinifera GN=VIT_05s0020g01120 PE=4 SV=1)

HSP 1 Score: 378.6 bits (971), Expect = 7.3e-102
Identity = 210/326 (64.42%), Postives = 249/326 (76.38%), Query Frame = 1


Query: 1   MLPLLAYQVISQYYRLQ-RNPITVGLLATNTLIHLRPNFLHPIIPSIEDVWFNAHLILKH 60
           MLPLLA    ++YYR+  + P+T GLLA NTLI+LRP+F+  ++P+I+ VWFN HLILKH
Sbjct: 12  MLPLLALHTANEYYRVGWKPPVTAGLLAANTLIYLRPSFIDSMLPTIDKVWFNPHLILKH 71

Query: 61  KELKRFLLSPFYHVGDHHLVYNMISLLWKGIQLETSMGSIEFASMVGALLLMSQGITLLL 120
           K+LKRFLLSPFYH+ + HLVYNM+SLLWKGIQLETSMGS EFASMV  LL MSQGITLLL
Sbjct: 72  KDLKRFLLSPFYHMDEAHLVYNMMSLLWKGIQLETSMGSAEFASMVATLLGMSQGITLLL 131

Query: 121 IKSLLVFFNYERSYFYEYSVGFSGVLFAMKVVLGSQAESYTYLHGLRVPSSHAAWLELIL 180
            KSLL+FF+YER+Y+ +++VGFSGVLFAMKVVL SQ+  YTY+HGL VP+ HAAW+ELIL
Sbjct: 132 AKSLLLFFDYERAYYSQFAVGFSGVLFAMKVVLNSQSADYTYVHGLIVPTRHAAWVELIL 191

Query: 181 IQMISPGVSFLGHLGGILAGLLFLHLKGTNLGLDPLTVLIRSVGRILSRPLGFFCRLNPF 240
           IQM  PGVSFLGHLGGILAG+L+L LKGT  G DPLT+L + +   LS PL F   L  F
Sbjct: 192 IQMFVPGVSFLGHLGGILAGILYLRLKGTYSGSDPLTILFKGLADALSWPLRFAQGL--F 251

Query: 241 RQRTIFGGGTVCRRQTAALERTTSIDTWRCQACTFDNSCVLSICEMCGTDRQDRLLS--- 300
           R+R I G GTV  RQT    RT+ +  WRC +CT+DN   LSICEMCGT R    LS   
Sbjct: 252 RRRRISGRGTVGGRQT---RRTSGV--WRCWSCTYDNPGGLSICEMCGTARNGNGLSSSL 311

Query: 301 --PHQLSHHFGLTREEVRRRRLERFG 321
             P Q SH   L  +E+RRRRLERFG
Sbjct: 312 QWPRQ-SHELPL--DELRRRRLERFG 327

[Close]

BLAST of Lsi01G020040 vs. TAIR10
Match: AT3G17611.1 (AT3G17611.1 RHOMBOID-like protein 14)

HSP 1 Score: 347.1 bits (889), Expect = 1.2e-95
Identity = 186/324 (57.41%), Postives = 233/324 (71.91%), Query Frame = 1


Query: 1   MLPLLAYQVISQYYRLQ-RNPITVGLLATNTLIHLRPNFLHPIIPSIEDVWFNAHLILKH 60
           MLPLLA   +++YYRL  + P+T  LLA NTL++LRP F+ P+IP I +VWFN HLI KH
Sbjct: 14  MLPLLALSAVAEYYRLPWKPPVTASLLAANTLVYLRPAFIDPVIPHISEVWFNPHLIFKH 73

Query: 61  KELKRFLLSPFYHVGDHHLVYNMISLLWKGIQLETSMGSIEFASMVGALLLMSQGITLLL 120
           K+LKR  LS FYHV + HLVYNM+SLLWKGI+LETSMGS EFASMV  L+ MSQG+TLLL
Sbjct: 74  KDLKRLFLSAFYHVNEPHLVYNMMSLLWKGIKLETSMGSSEFASMVFTLIGMSQGVTLLL 133

Query: 121 IKSLLVFFNYERSYFYEYSVGFSGVLFAMKVVLGSQAESYTYLHGLRVPSSHAAWLELIL 180
            KSLL+ F+Y+R+Y+ EY+VGFSGVLFAMKVVL SQAE Y+ ++G+ VP+ +AAW ELIL
Sbjct: 134 AKSLLLLFDYDRAYYNEYAVGFSGVLFAMKVVLNSQAEDYSSVYGILVPTKYAAWAELIL 193

Query: 181 IQMISPGVSFLGHLGGILAGLLFLHLKGTNLGLDPLTVLIRSVGRILSRPLGFFCRLNPF 240
           +QM  P  SFLGHLGGILAG+++L LKG+  G DP+T+ +R V R+++ PL F   +   
Sbjct: 194 VQMFVPNASFLGHLGGILAGIIYLKLKGSYSGSDPVTMAVRGVSRLVTWPLRFLNGMVRS 253

Query: 241 RQRTIFGGGTVCRRQTAALERTTSIDTWRCQACTFDNSCVLSICEMCGTDRQD----RLL 300
           R+R I G G V R QT           WRCQ+CT+DNS  LS CEMCG+ R       L 
Sbjct: 254 RRRRITGRGRVGRGQTG----IAGPGIWRCQSCTYDNSGWLSACEMCGSGRARGNGWSLN 313

Query: 301 SPHQLSHHFGLTREEVRRRRLERF 320
               LS    L  +E+RRRR+ERF
Sbjct: 314 QGPALSSSNDLPLDELRRRRVERF 333

[Close]

BLAST of Lsi01G020040 vs. TAIR10
Match: AT3G58460.2 (AT3G58460.2 RHOMBOID-like protein 15)

HSP 1 Score: 67.8 bits (164), Expect = 1.4e-11
Identity = 52/160 (32.50%), Postives = 85/160 (53.12%), Query Frame = 1


Query: 48  DVWFNAHLILKHKELKRFLLSPFYHVGDHHLVYNMISLLWKGIQLETSMGSIEFASMVGA 107
           +V F    I+   ++ RF  +  +H    H+++NM++L+  G +LE  MGS+    +   
Sbjct: 73  EVCFLPSAIISRFQVYRFYTAIIFHGSLLHVLFNMMALVPMGSELERIMGSVRLLYLT-V 132

Query: 108 LLLMSQGITLLLIKSLL---VFFNYERSYFYEYSVGFSGVLFAMKVVLGSQA--ESYTYL 167
           LL  +  +  LLI SL     F+ Y+     E ++GFSG+LF+M V+  S +   S +  
Sbjct: 133 LLATTNAVLHLLIASLAGYNPFYQYDH-LMNECAIGFSGILFSMIVIETSLSGVTSRSVF 192

Query: 168 HGLRVPSSHAAWLELILIQMISPGVSFLGHLGGILAGLLF 203
               VP+    W+ LI+ Q++   VS LGHL GIL+G  +
Sbjct: 193 GLFNVPAKLYPWILLIVFQLLMTNVSLLGHLCGILSGFSY 230

[Close]

BLAST of Lsi01G020040 vs. NCBI nr
Match: gi|659082947|ref|XP_008442110.1| (PREDICTED: uncharacterized protein At3g17611 [Cucumis melo])

HSP 1 Score: 525.4 bits (1352), Expect = 7.0e-146
Identity = 268/320 (83.75%), Postives = 287/320 (89.69%), Query Frame = 1


Query: 1   MLPLLAYQVISQYYRLQRNPITVGLLATNTLIHLRPNFLHPIIPSIEDVWFNAHLILKHK 60
           MLPLLAYQV+SQYYRLQ NPIT+GLLA+NTLIHLRPNFLH IIPSI++VWFNAHLILKHK
Sbjct: 1   MLPLLAYQVVSQYYRLQGNPITLGLLASNTLIHLRPNFLHHIIPSIDEVWFNAHLILKHK 60

Query: 61  ELKRFLLSPFYHVGDHHLVYNMISLLWKGIQLETSMGSIEFASMVGALLLMSQGITLLLI 120
           +LKRFLLSPFYHVGD HLVYNMISLLWKG QLETSMGSIEFASMVGALL+MSQGITLLLI
Sbjct: 61  DLKRFLLSPFYHVGDPHLVYNMISLLWKGTQLETSMGSIEFASMVGALLVMSQGITLLLI 120

Query: 121 KSLLVFFNYERSYFYEYSVGFSGVLFAMKVVLGSQAESYTYLHGLRVPSSHAAWLELILI 180
           KSLL+FFNY R Y+YEYSVGFSGVLFA+KVVLGS+ ESYTY+HGLRVPSSHAAWLELILI
Sbjct: 121 KSLLIFFNYGRPYYYEYSVGFSGVLFALKVVLGSETESYTYIHGLRVPSSHAAWLELILI 180

Query: 181 QMISPGVSFLGHLGGILAGLLFLHLKGTNLGLDPLTVLIRSVGRILSRPLGFFCRLNPFR 240
           QM+SPGVSFLGHLGGILAGLLFL LKGTN G DPLT+LIRSVGRILSRPLGFF RLNPFR
Sbjct: 181 QMVSPGVSFLGHLGGILAGLLFLRLKGTNSGSDPLTLLIRSVGRILSRPLGFFSRLNPFR 240

Query: 241 QRTIFGGGTVCRRQTAALERTTSIDTWRCQACTFDNSCVLSICEMCGTDRQDRLLSPHQL 300
           QR IFGGGTV  R        TSI TWRC+ACTFDNS +LS CEMCGTDRQD +L  +QL
Sbjct: 241 QRRIFGGGTVGSRP------VTSIGTWRCRACTFDNSGLLSTCEMCGTDRQDGVLHSNQL 300

Query: 301 SHHFGLTREEVRRRRLERFG 321
           S     TREE+RRRRLERFG
Sbjct: 301 SSQLDFTREEMRRRRLERFG 314

[Close]

BLAST of Lsi01G020040 vs. NCBI nr
Match: gi|449457305|ref|XP_004146389.1| (PREDICTED: uncharacterized protein At3g17611 [Cucumis sativus])

HSP 1 Score: 522.7 bits (1345), Expect = 4.5e-145
Identity = 267/320 (83.44%), Postives = 285/320 (89.06%), Query Frame = 1


Query: 1   MLPLLAYQVISQYYRLQRNPITVGLLATNTLIHLRPNFLHPIIPSIEDVWFNAHLILKHK 60
           MLPLLAYQV+SQYYRLQ NPIT+GLLA+NTLIHLRPNFLH IIPSI++VWFNAHLI KHK
Sbjct: 1   MLPLLAYQVVSQYYRLQGNPITLGLLASNTLIHLRPNFLHHIIPSIDEVWFNAHLIFKHK 60

Query: 61  ELKRFLLSPFYHVGDHHLVYNMISLLWKGIQLETSMGSIEFASMVGALLLMSQGITLLLI 120
           +LKRFLLSPFYHVGD HLVYNMISLLWKG QLETSMGSIEFASMVGALL+MSQGITLLLI
Sbjct: 61  DLKRFLLSPFYHVGDPHLVYNMISLLWKGTQLETSMGSIEFASMVGALLIMSQGITLLLI 120

Query: 121 KSLLVFFNYERSYFYEYSVGFSGVLFAMKVVLGSQAESYTYLHGLRVPSSHAAWLELILI 180
           KSLL+FFNY R Y+YEYSVGFSGVLFAMKVVLGS+ +SYTY+HGLRVPSSHAAWLELILI
Sbjct: 121 KSLLIFFNYGRPYYYEYSVGFSGVLFAMKVVLGSETDSYTYVHGLRVPSSHAAWLELILI 180

Query: 181 QMISPGVSFLGHLGGILAGLLFLHLKGTNLGLDPLTVLIRSVGRILSRPLGFFCRLNPFR 240
           QM+SPGVSFLGHLGGILAGLLFL LKGTN G DPLT+LIRSVGRILSR  GFFCRLNPFR
Sbjct: 181 QMVSPGVSFLGHLGGILAGLLFLRLKGTNSGSDPLTLLIRSVGRILSRSYGFFCRLNPFR 240

Query: 241 QRTIFGGGTVCRRQTAALERTTSIDTWRCQACTFDNSCVLSICEMCGTDRQDRLLSPHQL 300
           QR IFGGGTV  R        TSI TWRCQACTFDNS +LS CEMCGTDRQD +L  +QL
Sbjct: 241 QRRIFGGGTVGSRP------VTSIGTWRCQACTFDNSGLLSACEMCGTDRQDGVLHSNQL 300

Query: 301 SHHFGLTREEVRRRRLERFG 321
           S    LT EE+RRRRLERFG
Sbjct: 301 SSQLDLTWEEMRRRRLERFG 314

[Close]

BLAST of Lsi01G020040 vs. NCBI nr
Match: gi|802706995|ref|XP_012084232.1| (PREDICTED: uncharacterized protein At3g17611 [Jatropha curcas])

HSP 1 Score: 404.8 bits (1039), Expect = 1.4e-109
Identity = 214/322 (66.46%), Postives = 256/322 (79.50%), Query Frame = 1


Query: 1   MLPLLAYQVISQYYRLQ-RNPITVGLLATNTLIHLRPNFLHPIIPSIEDVWFNAHLILKH 60
           +LPLLA   +S+YYRL  + P+T GLLATNTLI+LRP+FLH I+PSI +VWFN++LILKH
Sbjct: 8   LLPLLAVHAVSEYYRLPWKPPVTAGLLATNTLIYLRPSFLHHILPSIHEVWFNSYLILKH 67

Query: 61  KELKRFLLSPFYHVGDHHLVYNMISLLWKGIQLETSMGSIEFASMVGALLLMSQGITLLL 120
           ++LKRF LSPFYHVG+ HLVYNMISLLWKGIQLE+SMGS EFASMV ALL MSQGITLLL
Sbjct: 68  RDLKRFFLSPFYHVGESHLVYNMISLLWKGIQLESSMGSAEFASMVAALLTMSQGITLLL 127

Query: 121 IKSLLVFFNYERSYFYEYSVGFSGVLFAMKVVLGSQAESYTYLHGLRVPSSHAAWLELIL 180
            KSLL+FF+Y R ++ EY+VGFSGVLFAMKVVL SQ+E YTY+HGL VP+ HAAW ELIL
Sbjct: 128 SKSLLLFFDYGRPFYSEYAVGFSGVLFAMKVVLNSQSEDYTYVHGLVVPARHAAWAELIL 187

Query: 181 IQMISPGVSFLGHLGGILAGLLFLHLKGTNLGLDPLTVLIRSVGRILSRPLGFFCRLNPF 240
           +QM  PGVSFLGHLGGILAG+L+L L+G +LG +PLT++I+++  IL+RPL F   L  F
Sbjct: 188 VQMFVPGVSFLGHLGGILAGILYLKLRGMHLGPNPLTLIIKNLAGILARPLRFLRGLFQF 247

Query: 241 RQRTIFGGGTVCRRQTAALERTTSIDTWRCQACTFDNSCVLSICEMCGTDRQDRLLSPHQ 300
           R++ I G GTV   Q     R+ S   WRCQACT+DNS  LS+CEMCGT R D   S  Q
Sbjct: 248 RRQQISGRGTVGGSQAG---RSVS-RVWRCQACTYDNSGWLSVCEMCGTTRGDNGSSSRQ 307

Query: 301 LSHHF-GLTREEVRRRRLERFG 321
            SH F   T EEVRRRR++RFG
Sbjct: 308 FSHQFDNPTMEEVRRRRIQRFG 325

[Close]

BLAST of Lsi01G020040 vs. NCBI nr
Match: gi|743910992|ref|XP_010999348.1| (PREDICTED: uncharacterized protein At3g17611 [Populus euphratica])

HSP 1 Score: 384.8 bits (987), Expect = 1.5e-103
Identity = 207/322 (64.29%), Postives = 247/322 (76.71%), Query Frame = 1


Query: 1   MLPLLAYQVISQYYRLQ-RNPITVGLLATNTLIHLRPNFLHPIIPSIEDVWFNAHLILKH 60
           MLPLLA   +S+YYRL  + P+T  LL  NTLI+LRP FL  I+PSI+ VWFN HLILK+
Sbjct: 11  MLPLLAIHAVSEYYRLPWKPPVTAALLGANTLIYLRPAFLRHILPSIDQVWFNPHLILKY 70

Query: 61  KELKRFLLSPFYHVGDHHLVYNMISLLWKGIQLETSMGSIEFASMVGALLLMSQGITLLL 120
           ++LKRFLLSPFYHV D HLVYNM+SLLWKGIQLETS+GS+EFASM+ ALL MSQGITLLL
Sbjct: 71  RDLKRFLLSPFYHVSDSHLVYNMMSLLWKGIQLETSVGSVEFASMLAALLAMSQGITLLL 130

Query: 121 IKSLLVFFNYERSYFYEYSVGFSGVLFAMKVVLGSQAESYTYLHGLRVPSSHAAWLELIL 180
            KSLL+FF+Y++ ++ EYS GFSGVLFAMKVVL SQ+ES T ++GL +P+ HAAW ELIL
Sbjct: 131 AKSLLLFFDYDKPFYSEYSAGFSGVLFAMKVVLNSQSESLTNVYGLVIPARHAAWAELIL 190

Query: 181 IQMISPGVSFLGHLGGILAGLLFLHLKGTNLGLDPLTVLIRSVGRILSRPLGFFCRLNPF 240
           IQM  PGVSFLGHLGGILAG+L+L LK    G DPLT+ IR++  ++S PL F   L  F
Sbjct: 191 IQMFVPGVSFLGHLGGILAGILYLKLKRAYSGPDPLTLTIRTLTNVISWPLRFVRNLFQF 250

Query: 241 RQRTIFGGGTVCRRQTAALERTTSIDTWRCQACTFDNSCVLSICEMCGTDRQDRLLSPHQ 300
           R+  I G G+V  RQT    RT S   WRCQACT+DNS +LS+CEMCGT R  R LS  +
Sbjct: 251 RRGRISGRGSVGGRQTG---RTMS-GLWRCQACTYDNSSMLSVCEMCGTSRGARGLSSRE 310

Query: 301 LSHHF-GLTREEVRRRRLERFG 321
            S H   LT EE+R RR+ERFG
Sbjct: 311 FSRHSDDLTLEEIRLRRIERFG 328

[Close]

BLAST of Lsi01G020040 vs. NCBI nr
Match: gi|224107010|ref|XP_002314342.1| (rhomboid family protein [Populus trichocarpa])

HSP 1 Score: 380.6 bits (976), Expect = 2.8e-102
Identity = 204/322 (63.35%), Postives = 246/322 (76.40%), Query Frame = 1


Query: 1   MLPLLAYQVISQYYRLQ-RNPITVGLLATNTLIHLRPNFLHPIIPSIEDVWFNAHLILKH 60
           MLPLLA   +S+YYRL  + P+T  LL  NTLI+LRP FL  I+PSI+ VWFN HLILK+
Sbjct: 11  MLPLLAIHAVSEYYRLPWKPPVTAALLGANTLIYLRPAFLRHILPSIDQVWFNPHLILKY 70

Query: 61  KELKRFLLSPFYHVGDHHLVYNMISLLWKGIQLETSMGSIEFASMVGALLLMSQGITLLL 120
           ++LKRFLLSPFYHV D HLVYNM+SLLWKGIQLETS+GS+EFASM+ ALL MSQGITLLL
Sbjct: 71  RDLKRFLLSPFYHVSDSHLVYNMMSLLWKGIQLETSVGSVEFASMLAALLAMSQGITLLL 130

Query: 121 IKSLLVFFNYERSYFYEYSVGFSGVLFAMKVVLGSQAESYTYLHGLRVPSSHAAWLELIL 180
            +SLL+FF+Y++ ++ EYS GFSGVLFAMKVVL SQ+ES T ++GL +P+ HAAW ELIL
Sbjct: 131 ARSLLLFFDYDKPFYSEYSAGFSGVLFAMKVVLNSQSESLTNVYGLVIPARHAAWAELIL 190

Query: 181 IQMISPGVSFLGHLGGILAGLLFLHLKGTNLGLDPLTVLIRSVGRILSRPLGFFCRLNPF 240
           IQM  PGVSFLGHLGGILAG+L++ LK    G DPLT+ IR++  ++S PL F   L  F
Sbjct: 191 IQMFVPGVSFLGHLGGILAGILYVKLKRAYSGPDPLTLTIRTLTNVISWPLRFVRNLFQF 250

Query: 241 RQRTIFGGGTVCRRQTAALERTTSIDTWRCQACTFDNSCVLSICEMCGTDRQDRLLSPHQ 300
           R+  I G G+V  RQT    R  S   WRCQACT+DNS +LS+CEMCGT R  R LS  +
Sbjct: 251 RRGRISGRGSVGGRQTG---RAMS-GLWRCQACTYDNSSLLSVCEMCGTSRGARGLSSRE 310

Query: 301 LSHHF-GLTREEVRRRRLERFG 321
            S H   LT EE+R RR+ERFG
Sbjct: 311 FSRHSDDLTLEEIRLRRIERFG 328

The following BLAST results are available for this feature:

BLAST of Lsi01G020040 vs. Swiss-Prot


Match Name	E-value	Identity	Description
RBL14_ARATH	2.1e-94	57.41	Rhomboid-like protein 14, mitochondrial OS=Arabidopsis thaliana GN=RBL14 PE=2 SV...	[more]
RHBL4_HUMAN	1.0e-24	31.58	Rhomboid-related protein 4 OS=Homo sapiens GN=RHBDD1 PE=1 SV=1	[more]
RHBL4_MOUSE	1.5e-23	35.68	Rhomboid-related protein 4 OS=Mus musculus GN=Rhbdd1 PE=1 SV=1	[more]
RHBL4_PONAB	1.3e-22	35.68	Rhomboid-related protein 4 OS=Pongo abelii GN=RHBDD1 PE=2 SV=1	[more]
RHBL4_RAT	4.8e-22	35.18	Rhomboid-related protein 4 OS=Rattus norvegicus GN=Rhbdd1 PE=2 SV=1	[more]

BLAST of Lsi01G020040 vs. TrEMBL


Match Name	E-value	Identity	Description
A0A0A0L173_CUCSA	3.1e-145	83.44	Uncharacterized protein OS=Cucumis sativus GN=Csa_4G506850 PE=4 SV=1	[more]
A0A067JVV7_JATCU	9.6e-110	66.46	Uncharacterized protein OS=Jatropha curcas GN=JCGZ_19107 PE=4 SV=1	[more]
A0A0A7E6S4_9AQUA	1.9e-102	63.04	Rhomboid protein Ilepu_RBL14 OS=Ilex purpurea PE=2 SV=1	[more]
B9HXC8_POPTR	1.9e-102	63.35	Rhomboid family protein OS=Populus trichocarpa GN=POPTR_0010s00720g PE=4 SV=1	[more]
A5BGK5_VITVI	7.3e-102	64.42	Putative uncharacterized protein OS=Vitis vinifera GN=VIT_05s0020g01120 PE=4 SV=...	[more]

BLAST of Lsi01G020040 vs. TAIR10


Match Name	E-value	Identity	Description
AT3G17611.1	1.2e-95	57.41	RHOMBOID-like protein 14	[more]
AT3G58460.2	1.4e-11	32.50	RHOMBOID-like protein 15	[more]

BLAST of Lsi01G020040 vs. NCBI nr


Match Name	E-value	Identity	Description
gi\|659082947\|ref\|XP_008442110.1\|	7.0e-146	83.75	PREDICTED: uncharacterized protein At3g17611 [Cucumis melo]	[more]
gi\|449457305\|ref\|XP_004146389.1\|	4.5e-145	83.44	PREDICTED: uncharacterized protein At3g17611 [Cucumis sativus]	[more]
gi\|802706995\|ref\|XP_012084232.1\|	1.4e-109	66.46	PREDICTED: uncharacterized protein At3g17611 [Jatropha curcas]	[more]
gi\|743910992\|ref\|XP_010999348.1\|	1.5e-103	64.29	PREDICTED: uncharacterized protein At3g17611 [Populus euphratica]	[more]
gi\|224107010\|ref\|XP_002314342.1\|	2.8e-102	63.35	rhomboid family protein [Populus trichocarpa]	[more]

Annotated Terms

The following terms have been associated with this gene:

Vocabulary: **Cellular Component**
Term	Definition
GO:0016021	integral component of membrane

Vocabulary: **Molecular Function**
Term	Definition
GO:0004252	serine-type endopeptidase activity
GO:0008270	zinc ion binding

Vocabulary: **INTERPRO**
Term	Definition
IPR022764	Peptidase_S54_rhomboid_dom
IPR001876	Znf_RanBP2

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0006508	proteolysis
biological_process	GO:0008150	biological_process
cellular_component	GO:0016021	integral component of membrane
molecular_function	GO:0004252	serine-type endopeptidase activity
molecular_function	GO:0008270	zinc ion binding
molecular_function	GO:0046872	metal ion binding

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Lsi01G020040.1	Lsi01G020040.1	mRNA

InterPro

Analysis Name: InterPro Annotations of Lagenaria siceraria
Date Performed: 2017-09-18


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR001876	Zinc finger, RanBP2-type	PROSITE	PS01358	ZF_RANBP2_1	coord: 267..286 scor
IPR001876	Zinc finger, RanBP2-type	PROFILE	PS50199	ZF_RANBP2_2	coord: 263..292 score: 8
IPR001876	Zinc finger, RanBP2-type	unknown	SSF90209	Ran binding protein zinc finger-like	coord: 265..290 score: 5.
IPR022764	Peptidase S54, rhomboid domain	GENE3D	G3DSA:1.20.1540.10		coord: 19..202 score: 2.8
IPR022764	Peptidase S54, rhomboid domain	PFAM	PF01694	Rhomboid	coord: 58..203 score: 1.6
None	No IPR available	PANTHER	PTHR12917	ASPARTYL PROTEASE DDI-RELATED	coord: 1..319 score: 4.0
None	No IPR available	PANTHER	PTHR12917:SF18	RHOMBOID DOMAIN-CONTAINING PROTEIN 3	coord: 1..319 score: 4.0
None	No IPR available	unknown	SSF144091	Rhomboid-like	coord: 18..203 score: 9.81

Synteny

The following gene(s) are orthologous to this gene:


Gene	Orthologue	Organism	Block
Lsi01G020040	Csa4G506850	Cucumber (Chinese Long) v2	culsiB250
Lsi01G020040	CSPI04G20320	Wild cucumber (PI 183967)	cpilsiB263
Lsi01G020040	Cla011678	Watermelon (97103) v1	lsiwmB146
Lsi01G020040	MELO3C008914	Melon (DHL92) v3.5.1	lsimeB152
Lsi01G020040	ClCG04G002200	Watermelon (Charleston Gray)	lsiwcgB135
Lsi01G020040	MELO3C008914.2	Melon (DHL92) v3.6.1	lsimedB159
Lsi01G020040	Cla97C04G069770	Watermelon (97103) v2	lsiwmbB133
Lsi01G020040	CsGy4G018930	Cucumber (Gy14) v2	cgyblsiB243
Lsi01G020040	CsaV3_4G030510	Cucumber (Chinese Long) v3	cuclsiB279

The following gene(s) are paralogous to this gene:

None

The following block(s) are covering this gene:


Gene	Organism	Block
Lsi01G020040	Silver-seed gourd	carlsiB259
Lsi01G020040	Silver-seed gourd	carlsiB430
Lsi01G020040	Watermelon (97103) v2	lsiwmbB136
Lsi01G020040	Wax gourd	lsiwgoB173
Lsi01G020040	Cucurbita maxima (Rimu)	cmalsiB792
Lsi01G020040	Cucurbita moschata (Rifu)	cmolsiB589
Lsi01G020040	Cucurbita moschata (Rifu)	cmolsiB780
Lsi01G020040	Cucurbita pepo (Zucchini)	cpelsiB392
Lsi01G020040	Cucurbita pepo (Zucchini)	cpelsiB399