CSPI04G20320 (gene) Wild cucumber (PI 183967)

NameCSPI04G20320
Typegene
OrganismCucumis sativus (Wild cucumber (PI 183967))
DescriptionRhomboid family protein
LocationChr4 : 18429919 .. 18433528 (+)
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: five_prime_UTRCDSthree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
GGAAAAGAACATTGGTGAGTTTTCTAGAGATTTCGTAGTCGGCAAGGCAATCCAAGGTGGAGTTCTTAATCGGTGTGACATCGGAGTTATTTTGTACGTCGACACTCTTTCCCTTTGGTCAAATACTCTACTTTGGTGCTCCTTCGTTCTTTTCTGCTCTTTCAAGTTTCAACAGGTAAGATTCACGGTTAATTCTCAATTTCCTGCTTTCAAACCCCATTTCCTTGATTTCTTCACTCCTCGATTTATTTCTTTTCGCAAAATATGGACTATCTTTTGATAGAATCACGATTGTGTCATCAATTTAGTGAATTTGTTTTTTCCTCTGTTGCATGTTCTTGGTTTTGTGATTGTATAATGGGAACACTTGCTGCTCGCTCGTTGTTCAAATTATTGCTTTTTTTTAGGCTTTGAATACTCGAATGCCATTTCTCAATAGGTGTAGTTTTTATGTTTTGATCTTTAATGGTTGCGAGCTATGTGAGTTACCATAGATGATTTGTTTTGTGTTAAGCTATGTGGACTGCTTTAAAATTGAATTTATTTGGATTTGGAATGGTCTTGAATTTTGAGGATTTGGTACTGCTAATTATTTATAGGGGTTCCATTTTGGAAGTAGGACTATAGGTTTCCAGCCTAATATTTTGTATATCGTGTATGCACAACCTTGGCCGTTTCCTGTACATAATATAAAAATCACAACCAATAAAATGGCACATGTCAAAATCGACCTAATGACACGTCAGAGATTGGAGAGATATTTTTAGCAAGCAGTGGACGCAAGGCTAAAAGTCATCTTCAGCTATGCCAAACTTTTAATGCATTGCTATGGCAACCCAGTTCTTCCACTTGAACCTTAACTATAATTTGGTATTTATTTCTTCCTGAAATAGATACTACATTTATGTAAGAATAAGGGTTTGCTTCAGGAAGTTTGTGTGAGGACCCTAGATAGGAAAACAGCATAGAAAATGAAGATGGTTACTGGTGGGGCATGTTAGCGAATAGTTAGTAAGTTAGTTAGGTCTTTTTGTTATAAATAGTAGGAGAGTGGTTGAGGAGAGGTGTGAAGAATTTTGTGGAGGGATTTCCTTGTAAAGGGAATTTGGGAGAGTCCAGCCCTCTCGAAAGGTTAGGTTACTTTGTTGCTTGTCTTGTGGTATTCTAGAATATATTACATATATTTTCATATTTGATTGTTCTATAACTGTTCTTGAGCTCATCCTTTGTTCTTGAGATAGTTCTTGTTAGGAGGGATCTTAACAGTTTGGTACCCTAGTTATATCTATATATTTTTTTTGAAACAATATTTTCTTGAGTTGCAATGCATGAGAATGCTGCTCTTTCCTCTTTGTATTATTGTTCCTACTTTAACCAACTTTTTATTGGATACTATAGGGAATAAGCACAGGCAGGAATGTTACCACTGCTAGCTTACCAAGTAGTGAGCCAATACTACAGGCTCCAAGGGAACCCGATCACTCTGGGGCTCCTTGCCTCTAATACTCTCATTCATCTAAGGCCCAATTTTCTTCATCATATCATCCCTTCCATAGATGAGGTCTGGTTTAATGCACATCTCATCTTCAAGGTGACTATTCTGGCTAATAATCTAATTCTATTCTGCTGTTCTCTGTTTAAGACTTAACTGCACTCTCTATGTTAGAGTTAATTGGTGATGATTATAAAAATTTAATGAGAGATCTTTCTGCTGAGGACTTTCCTATTCATTAATACATCGGTAGGGAAGTCCTAAACAGAATGGCACGTTACTTTCTCTGGAATAAAGCATTGCTGTCTCATTGAGAAATTGGTATCATGTTTCTTTTTTGATCAGCTGTATAAATAATCTTGTAGTTTCCTTAGATACTGACTACCTGCTTTATAGCATCATTGAAGCGAATAGGTCTTTATTTATTTATTGAACTTTTTAGCTGTGAAGTCATTTTTCATTGAGAAAAATGGAAGAAATAAGTAGATACTAGTTGAATTTGTATGCTTTAACCATAAGAAATGGTTCTTGTATGATGTTGAACGTTACAAAACACATATAGATTGGGTAGCCCCCACATAACTGTGTAATTTGTGTCCTTACAAATCGTCCTCATAGTGAGGTTGTTGTATGATGGCCTCAAATTATAGTTAAACTTTTATTTTGAGAAAAATGAAAAGCTTTTAAGAGTTTGCTTTGATTTTTGTACAATATTCACAGTTTCTTTCTTTTCAGTATTGTACTTCTTTAATCCATCTTGATGAGTATATACCTAACAATTTTGAACTCCTGTAGGATTATTTTCTTTTTTGTTGTTTATTAACTTGTTTGCATGTCGTTTTTGAATGAGATAATGTGATATTGGCATTCCTGTTACTGTCATAATATTGGTATCATTTGCATTTCCCACTTTGCCAGCACAAGGACTTGAAGCGTTTTTTACTATCACCATTCTACCATGTGGGTGACCCTCACCTTGTCTACAACATGATATCTCTACTATGGAAAGGCACTCAGTTGGAAACATCAATGGGTAGCATCGAATTTGCTTCAATGGTTGGTGCATTACTCATAATGTCGCAGGGAATTACCTTGTTGCTCATCAAATCCTTACTGATATTCTTTAATTATGGGAGGCCTTACTACTATGAATATTCTGTTGGGTTTTCCGGTGTACTTTTTGCTATGAAAGTTGTTCTTGGTTCTGAAACTGACAGCTACACTTACGTTTATGGTCTAAGAGTACCATCAAGTCATGCTGCATGGTTGGAACTAATACTTATCCAAATGGTTTCACCTGGTGTCTCTTTCCTTGGTCATCTTGGTGGTATACTTGCTGGTCTTCTCTTTTTGCGTCTGAAGGGTACGAATTCAGGGTCGGATCCATTGACACTTCTTATTAGAAGTGTTGGTCGAATATTGAGTCGTTCTTATGGATTTTTCTGCCGCTTGAATCCCTTTCGGCAGCGTAGGATATTTGGAGGAGGAACGGTCGGTTCAAGGCCTGTAACGAGCATCGGCACATGGAGGTGTCAGGCATGTACATTTGACAATTCTGGTTTATTAAGTGCGTGTGAAATGTGTGGGACAGATCGGCAGGATGGGGTGCTGCATTCTAACCAATTATCGAGTCAATTGGATTTGACTTGGGAGGAAATGCGTCGTCGGAGACTTGAAAGATTTGGTTGATGGTAGAATAGATTGTTCATTTTCTCAGGCCACCAGATATTTACTTTTAGAAGAGTAAATTGAAAGGCTACTAGATGTTGAAGTTGTTTACACCATCTTTCGGAAATGATGAAGTTGTTTACTAGACGTTGAAAAAATGAAGTGGTATGGAGCATGGAGCTTGTGTTTAGGAACATAGCAAATTTGATTGAACACAGATAGAAAAGTCTTTGTACACGGGGAAGGAATTAACATACTTTCTTTTCATCATTGGTTTTCTATATGACATCTCTTCATGTAGCTGCTTCTTGATAACGGTTGAATAGATTTCTAATTGAAGTAATTGTTACACGAATGGCATCTATGATTGTTGTTGCAAGTTTTTCTAATGTGAAGGTTAATAATCAACCAAAAACTGACATTGCTAATGAACGGTTTGTCCTTTAATTG

mRNA sequence

ATGTTACCACTGCTAGCTTACCAAGTAGTGAGCCAATACTACAGGCTCCAAGGGAACCCGATCACTCTGGGGCTCCTTGCCTCTAATACTCTCATTCATCTAAGGCCCAATTTTCTTCATCATATCATCCCTTCCATAGATGAGGTCTGGTTTAATGCACATCTCATCTTCAAGCACAAGGACTTGAAGCGTTTTTTACTATCACCATTCTACCATGTGGGTGACCCTCACCTTGTCTACAACATGATATCTCTACTATGGAAAGGCACTCAGTTGGAAACATCAATGGGTAGCATCGAATTTGCTTCAATGGTTGGTGCATTACTCATAATGTCGCAGGGAATTACCTTGTTGCTCATCAAATCCTTACTGATATTCTTTAATTATGGGAGGCCTTACTACTATGAATATTCTGTTGGGTTTTCCGGTGTACTTTTTGCTATGAAAGTTGTTCTTGGTTCTGAAACTGACAGCTACACTTACGTTTATGGTCTAAGAGTACCATCAAGTCATGCTGCATGGTTGGAACTAATACTTATCCAAATGGTTTCACCTGGTGTCTCTTTCCTTGGTCATCTTGGTGGTATACTTGCTGGTCTTCTCTTTTTGCGTCTGAAGGGTACGAATTCAGGGTCGGATCCATTGACACTTCTTATTAGAAGTGTTGGTCGAATATTGAGTCGTTCTTATGGATTTTTCTGCCGCTTGAATCCCTTTCGGCAGCGTAGGATATTTGGAGGAGGAACGGTCGGTTCAAGGCCTGTAACGAGCATCGGCACATGGAGGTGTCAGGCATGTACATTTGACAATTCTGGTTTATTAAGTGCGTGTGAAATGTGTGGGACAGATCGGCAGGATGGGGTGCTGCATTCTAACCAATTATCGAGTCAATTGGATTTGACTTGGGAGGAAATGCGTCGTCGGAGACTTGAAAGATTTGGTTGA

Coding sequence (CDS)

ATGTTACCACTGCTAGCTTACCAAGTAGTGAGCCAATACTACAGGCTCCAAGGGAACCCGATCACTCTGGGGCTCCTTGCCTCTAATACTCTCATTCATCTAAGGCCCAATTTTCTTCATCATATCATCCCTTCCATAGATGAGGTCTGGTTTAATGCACATCTCATCTTCAAGCACAAGGACTTGAAGCGTTTTTTACTATCACCATTCTACCATGTGGGTGACCCTCACCTTGTCTACAACATGATATCTCTACTATGGAAAGGCACTCAGTTGGAAACATCAATGGGTAGCATCGAATTTGCTTCAATGGTTGGTGCATTACTCATAATGTCGCAGGGAATTACCTTGTTGCTCATCAAATCCTTACTGATATTCTTTAATTATGGGAGGCCTTACTACTATGAATATTCTGTTGGGTTTTCCGGTGTACTTTTTGCTATGAAAGTTGTTCTTGGTTCTGAAACTGACAGCTACACTTACGTTTATGGTCTAAGAGTACCATCAAGTCATGCTGCATGGTTGGAACTAATACTTATCCAAATGGTTTCACCTGGTGTCTCTTTCCTTGGTCATCTTGGTGGTATACTTGCTGGTCTTCTCTTTTTGCGTCTGAAGGGTACGAATTCAGGGTCGGATCCATTGACACTTCTTATTAGAAGTGTTGGTCGAATATTGAGTCGTTCTTATGGATTTTTCTGCCGCTTGAATCCCTTTCGGCAGCGTAGGATATTTGGAGGAGGAACGGTCGGTTCAAGGCCTGTAACGAGCATCGGCACATGGAGGTGTCAGGCATGTACATTTGACAATTCTGGTTTATTAAGTGCGTGTGAAATGTGTGGGACAGATCGGCAGGATGGGGTGCTGCATTCTAACCAATTATCGAGTCAATTGGATTTGACTTGGGAGGAAATGCGTCGTCGGAGACTTGAAAGATTTGGTTGA
BLAST of CSPI04G20320 vs. Swiss-Prot
Match: RBL14_ARATH (Rhomboid-like protein 14, mitochondrial OS=Arabidopsis thaliana GN=RBL14 PE=2 SV=1)

HSP 1 Score: 373.6 bits (958), Expect = 2.1e-102
Identity = 193/320 (60.31%), Postives = 237/320 (74.06%), Query Frame = 1

Query: 1   MLPLLAYQVVSQYYRLQGNP-ITLGLLASNTLIHLRPNFLHHIIPSIDEVWFNAHLIFKH 60
           MLPLLA   V++YYRL   P +T  LLA+NTL++LRP F+  +IP I EVWFN HLIFKH
Sbjct: 14  MLPLLALSAVAEYYRLPWKPPVTASLLAANTLVYLRPAFIDPVIPHISEVWFNPHLIFKH 73

Query: 61  KDLKRFLLSPFYHVGDPHLVYNMISLLWKGTQLETSMGSIEFASMVGALLIMSQGITLLL 120
           KDLKR  LS FYHV +PHLVYNM+SLLWKG +LETSMGS EFASMV  L+ MSQG+TLLL
Sbjct: 74  KDLKRLFLSAFYHVNEPHLVYNMMSLLWKGIKLETSMGSSEFASMVFTLIGMSQGVTLLL 133

Query: 121 IKSLLIFFNYGRPYYYEYSVGFSGVLFAMKVVLGSETDSYTYVYGLRVPSSHAAWLELIL 180
            KSLL+ F+Y R YY EY+VGFSGVLFAMKVVL S+ + Y+ VYG+ VP+ +AAW ELIL
Sbjct: 134 AKSLLLLFDYDRAYYNEYAVGFSGVLFAMKVVLNSQAEDYSSVYGILVPTKYAAWAELIL 193

Query: 181 IQMVSPGVSFLGHLGGILAGLLFLRLKGTNSGSDPLTLLIRSVGRILSRSYGFFCRLNPF 240
           +QM  P  SFLGHLGGILAG+++L+LKG+ SGSDP+T+ +R V R+++    F   +   
Sbjct: 194 VQMFVPNASFLGHLGGILAGIIYLKLKGSYSGSDPVTMAVRGVSRLVTWPLRFLNGMVRS 253

Query: 241 RQRRIFGGGTV--GSRPVTSIGTWRCQACTFDNSGLLSACEMCGTDRQDG----VLHSNQ 300
           R+RRI G G V  G   +   G WRCQ+CT+DNSG LSACEMCG+ R  G    +     
Sbjct: 254 RRRRITGRGRVGRGQTGIAGPGIWRCQSCTYDNSGWLSACEMCGSGRARGNGWSLNQGPA 313

Query: 301 LSSQLDLTWEEMRRRRLERF 314
           LSS  DL  +E+RRRR+ERF
Sbjct: 314 LSSSNDLPLDELRRRRVERF 333

BLAST of CSPI04G20320 vs. Swiss-Prot
Match: RHBL4_MOUSE (Rhomboid-related protein 4 OS=Mus musculus GN=Rhbdd1 PE=1 SV=1)

HSP 1 Score: 120.9 bits (302), Expect = 2.4e-26
Identity = 101/316 (31.96%), Postives = 150/316 (47.47%), Query Frame = 1

Query: 9   VVSQYYRLQGN---PITLGLLASNTLIHLRP-NFLHHIIPSIDEVWFNAHLIFKHKDLKR 68
           ++SQ +++  N   P+TL  LA N    L P   L+H   S+++        ++ KD +R
Sbjct: 15  LLSQVFQIGINNIPPVTLATLAVNVWFFLNPWKPLYHSCISVEKC-------YQQKDWQR 74

Query: 69  FLLSPFYHVGDPHLVYNMISLLWKGTQLETSMGSIEFASMVGALLIMSQGITLLLIKSLL 128
            LLSP +H  D HL +NM+S+LWKG +LE  +GS  FA ++    +++ G+  LL++  +
Sbjct: 75  LLLSPLHHGDDWHLYFNMVSMLWKGVKLERRLGSRWFAYVIATFSLLT-GVVYLLLQFTV 134

Query: 129 IFFNYGRPYYYEYSVGFSGVLFAMKVVLGSE-TDSYTYVYGLRVPSSHAAWLELILIQMV 188
                   +    +VGFSGVLFA+KV+        +  + G  VP+  A W EL+ I   
Sbjct: 135 AELLNQPDFKRNCAVGFSGVLFALKVLSNHYCPGGFVNILGFPVPNRFACWAELVAIHFC 194

Query: 189 SPGVSFLGHLGGILAGLLFLRLKGTNSGSDPLTLLIRSVGRILSRSYGFFCRLNPFRQRR 248
           +PG SF GHL GIL GL++ +         PL  ++ +   I     G   + N F    
Sbjct: 195 TPGTSFAGHLAGILVGLMYTQ--------GPLKKIMDTCAGIFISHAGPSGQQNHFNNAG 254

Query: 249 IFG--GGTVGSRPVTSIGTWR----CQACTFDNSGLLSACEMCGTDRQDGVLHSNQLSSQ 308
             G        RPVT   T+R      A   +   L  A      DR  G   +  +   
Sbjct: 255 PSGYQNHYADGRPVTYDATYRNYDVYTAGLSEEEQLERALRASIWDR--GNTRNGPMPYG 312

Query: 309 LDLTWEEMRRRRLERF 314
             L  EEMRR+RL RF
Sbjct: 315 FRLPPEEMRRQRLHRF 312

BLAST of CSPI04G20320 vs. Swiss-Prot
Match: RHBL4_HUMAN (Rhomboid-related protein 4 OS=Homo sapiens GN=RHBDD1 PE=1 SV=1)

HSP 1 Score: 116.3 bits (290), Expect = 6.0e-25
Identity = 81/234 (34.62%), Postives = 120/234 (51.28%), Query Frame = 1

Query: 20  PITLGLLASNTLIHLRPNF-LHHIIPSIDEVWFNAHLIFKHKDLKRFLLSPFYHVGDPHL 79
           P+TL  LA N    L P   L+    S+++        ++ KD +R LLSP +H  D HL
Sbjct: 29  PVTLATLALNIWFFLNPQKPLYSSCLSVEKC-------YQQKDWQRLLLSPLHHADDWHL 88

Query: 80  VYNMISLLWKGTQLETSMGSIEFASMVGALLIMSQGITLLLIKSLLIFFNYGRPYYYEYS 139
            +NM S+LWKG  LE  +GS  FA ++ A  +++ G+  LL++  +  F     +    +
Sbjct: 89  YFNMASMLWKGINLERRLGSRWFAYVITAFSVLT-GVVYLLLQFAVAEFMDEPDFKRSCA 148

Query: 140 VGFSGVLFAMKVVLGSE-TDSYTYVYGLRVPSSHAAWLELILIQMVSPGVSFLGHLGGIL 199
           VGFSGVLFA+KV+        +  + G  VP+  A W+EL+ I + SPG SF GHL GIL
Sbjct: 149 VGFSGVLFALKVLNNHYCPGGFVNILGFPVPNRFACWVELVAIHLFSPGTSFAGHLAGIL 208

Query: 200 AGLLFLRLKGTNSGSDPLTLLIRSVGRILSRSYGFFCRLNPFRQRRIFGGGTVG 252
            GL++ +         PL  ++ +     S S G+     P RQ      G+ G
Sbjct: 209 VGLMYTQ--------GPLKKIMEACAGGFSSSVGY-----PGRQYYFNSSGSSG 241

BLAST of CSPI04G20320 vs. Swiss-Prot
Match: RHBL4_RAT (Rhomboid-related protein 4 OS=Rattus norvegicus GN=Rhbdd1 PE=2 SV=1)

HSP 1 Score: 109.8 bits (273), Expect = 5.6e-23
Identity = 71/199 (35.68%), Postives = 107/199 (53.77%), Query Frame = 1

Query: 9   VVSQYYRLQGN---PITLGLLASNTLIHLRP-NFLHHIIPSIDEVWFNAHLIFKHKDLKR 68
           ++SQ + +  N   P+TL  LA N    L P   L+H   S+++        ++  D +R
Sbjct: 15  LLSQVFHIGINNIPPVTLATLAVNVWFFLNPWKPLYHSCISVEKC-------YQQNDWQR 74

Query: 69  FLLSPFYHVGDPHLVYNMISLLWKGTQLETSMGSIEFASMVGALLIMSQGITLLLIKSLL 128
            LLSP +H  D HL +NM+S+LWKG +LE  +GS  FA ++    +++ G+  LL++   
Sbjct: 75  LLLSPVHHGDDWHLYFNMVSMLWKGVKLEKRLGSRWFAYIIATFSLLT-GVVYLLLQFAS 134

Query: 129 IFFNYGRPYYYEYSVGFSGVLFAMKVVLGSE-TDSYTYVYGLRVPSSHAAWLELILIQMV 188
                   +    +VGFSGVLFA+KV+        +  + G  VP+  A W EL  I   
Sbjct: 135 AELMNQPDFKRNCAVGFSGVLFALKVLSNHYCPGGFVNILGFPVPNRFACWAELAAIHFC 194

Query: 189 SPGVSFLGHLGGILAGLLF 203
           +PG SF GHL GIL GL++
Sbjct: 195 TPGTSFAGHLAGILVGLMY 205

BLAST of CSPI04G20320 vs. Swiss-Prot
Match: RHBL4_PONAB (Rhomboid-related protein 4 OS=Pongo abelii GN=RHBDD1 PE=2 SV=1)

HSP 1 Score: 107.5 bits (267), Expect = 2.8e-22
Identity = 67/185 (36.22%), Postives = 101/185 (54.59%), Query Frame = 1

Query: 20  PITLGLLASNTLIHLRPNF-LHHIIPSIDEVWFNAHLIFKHKDLKRFLLSPFYHVGDPHL 79
           P+TL  LA N    L P   L+    S+++        ++ +D +R LLSP +H  D HL
Sbjct: 29  PVTLATLALNIWFFLNPQKPLYSSCLSVEKC-------YQQRDWQRLLLSPLHHADDWHL 88

Query: 80  VYNMISLLWKGTQLETSMGSIEFASMVGALLIMSQGITLLLIKSLLIFFNYGRPYYYEYS 139
            +N  S+LWKG  LE  +GS  FA ++    +++ G+  LL++  +  F     +    +
Sbjct: 89  YFNTASVLWKGINLERRLGSRWFAYVITTFSVLT-GVVYLLLQFAVAEFMDEPDFKRSCA 148

Query: 140 VGFSGVLFAMKVVLGSE-TDSYTYVYGLRVPSSHAAWLELILIQMVSPGVSFLGHLGGIL 199
           VGFSGVLFA+KV+        +  + G  VP+  A W+EL+ I + SPG SF GH  GIL
Sbjct: 149 VGFSGVLFALKVLNNHYCPGGFVNILGFPVPNRFACWVELVAIHLFSPGTSFAGHQAGIL 205

Query: 200 AGLLF 203
            GL++
Sbjct: 209 VGLMY 205

BLAST of CSPI04G20320 vs. TrEMBL
Match: A0A0A0L173_CUCSA (Uncharacterized protein OS=Cucumis sativus GN=Csa_4G506850 PE=4 SV=1)

HSP 1 Score: 630.2 bits (1624), Expect = 1.4e-177
Identity = 313/314 (99.68%), Postives = 314/314 (100.00%), Query Frame = 1

Query: 1   MLPLLAYQVVSQYYRLQGNPITLGLLASNTLIHLRPNFLHHIIPSIDEVWFNAHLIFKHK 60
           MLPLLAYQVVSQYYRLQGNPITLGLLASNTLIHLRPNFLHHIIPSIDEVWFNAHLIFKHK
Sbjct: 1   MLPLLAYQVVSQYYRLQGNPITLGLLASNTLIHLRPNFLHHIIPSIDEVWFNAHLIFKHK 60

Query: 61  DLKRFLLSPFYHVGDPHLVYNMISLLWKGTQLETSMGSIEFASMVGALLIMSQGITLLLI 120
           DLKRFLLSPFYHVGDPHLVYNMISLLWKGTQLETSMGSIEFASMVGALLIMSQGITLLLI
Sbjct: 61  DLKRFLLSPFYHVGDPHLVYNMISLLWKGTQLETSMGSIEFASMVGALLIMSQGITLLLI 120

Query: 121 KSLLIFFNYGRPYYYEYSVGFSGVLFAMKVVLGSETDSYTYVYGLRVPSSHAAWLELILI 180
           KSLLIFFNYGRPYYYEYSVGFSGVLFAMKVVLGSETDSYTYV+GLRVPSSHAAWLELILI
Sbjct: 121 KSLLIFFNYGRPYYYEYSVGFSGVLFAMKVVLGSETDSYTYVHGLRVPSSHAAWLELILI 180

Query: 181 QMVSPGVSFLGHLGGILAGLLFLRLKGTNSGSDPLTLLIRSVGRILSRSYGFFCRLNPFR 240
           QMVSPGVSFLGHLGGILAGLLFLRLKGTNSGSDPLTLLIRSVGRILSRSYGFFCRLNPFR
Sbjct: 181 QMVSPGVSFLGHLGGILAGLLFLRLKGTNSGSDPLTLLIRSVGRILSRSYGFFCRLNPFR 240

Query: 241 QRRIFGGGTVGSRPVTSIGTWRCQACTFDNSGLLSACEMCGTDRQDGVLHSNQLSSQLDL 300
           QRRIFGGGTVGSRPVTSIGTWRCQACTFDNSGLLSACEMCGTDRQDGVLHSNQLSSQLDL
Sbjct: 241 QRRIFGGGTVGSRPVTSIGTWRCQACTFDNSGLLSACEMCGTDRQDGVLHSNQLSSQLDL 300

Query: 301 TWEEMRRRRLERFG 315
           TWEEMRRRRLERFG
Sbjct: 301 TWEEMRRRRLERFG 314

BLAST of CSPI04G20320 vs. TrEMBL
Match: A0A067JVV7_JATCU (Uncharacterized protein OS=Jatropha curcas GN=JCGZ_19107 PE=4 SV=1)

HSP 1 Score: 415.6 bits (1067), Expect = 5.3e-113
Identity = 214/320 (66.88%), Postives = 256/320 (80.00%), Query Frame = 1

Query: 1   MLPLLAYQVVSQYYRLQGNP-ITLGLLASNTLIHLRPNFLHHIIPSIDEVWFNAHLIFKH 60
           +LPLLA   VS+YYRL   P +T GLLA+NTLI+LRP+FLHHI+PSI EVWFN++LI KH
Sbjct: 8   LLPLLAVHAVSEYYRLPWKPPVTAGLLATNTLIYLRPSFLHHILPSIHEVWFNSYLILKH 67

Query: 61  KDLKRFLLSPFYHVGDPHLVYNMISLLWKGTQLETSMGSIEFASMVGALLIMSQGITLLL 120
           +DLKRF LSPFYHVG+ HLVYNMISLLWKG QLE+SMGS EFASMV ALL MSQGITLLL
Sbjct: 68  RDLKRFFLSPFYHVGESHLVYNMISLLWKGIQLESSMGSAEFASMVAALLTMSQGITLLL 127

Query: 121 IKSLLIFFNYGRPYYYEYSVGFSGVLFAMKVVLGSETDSYTYVYGLRVPSSHAAWLELIL 180
            KSLL+FF+YGRP+Y EY+VGFSGVLFAMKVVL S+++ YTYV+GL VP+ HAAW ELIL
Sbjct: 128 SKSLLLFFDYGRPFYSEYAVGFSGVLFAMKVVLNSQSEDYTYVHGLVVPARHAAWAELIL 187

Query: 181 IQMVSPGVSFLGHLGGILAGLLFLRLKGTNSGSDPLTLLIRSVGRILSRSYGFFCRLNPF 240
           +QM  PGVSFLGHLGGILAG+L+L+L+G + G +PLTL+I+++  IL+R   F   L  F
Sbjct: 188 VQMFVPGVSFLGHLGGILAGILYLKLRGMHLGPNPLTLIIKNLAGILARPLRFLRGLFQF 247

Query: 241 RQRRIFGGGTVGS----RPVTSIGTWRCQACTFDNSGLLSACEMCGTDRQDGVLHSNQLS 300
           R+++I G GTVG     R V+ +  WRCQACT+DNSG LS CEMCGT R D    S Q S
Sbjct: 248 RRQQISGRGTVGGSQAGRSVSRV--WRCQACTYDNSGWLSVCEMCGTTRGDNGSSSRQFS 307

Query: 301 SQLD-LTWEEMRRRRLERFG 315
            Q D  T EE+RRRR++RFG
Sbjct: 308 HQFDNPTMEEVRRRRIQRFG 325

BLAST of CSPI04G20320 vs. TrEMBL
Match: B9HXC8_POPTR (Rhomboid family protein OS=Populus trichocarpa GN=POPTR_0010s00720g PE=4 SV=1)

HSP 1 Score: 397.1 bits (1019), Expect = 2.0e-107
Identity = 207/318 (65.09%), Postives = 243/318 (76.42%), Query Frame = 1

Query: 1   MLPLLAYQVVSQYYRLQGNP-ITLGLLASNTLIHLRPNFLHHIIPSIDEVWFNAHLIFKH 60
           MLPLLA   VS+YYRL   P +T  LL +NTLI+LRP FL HI+PSID+VWFN HLI K+
Sbjct: 11  MLPLLAIHAVSEYYRLPWKPPVTAALLGANTLIYLRPAFLRHILPSIDQVWFNPHLILKY 70

Query: 61  KDLKRFLLSPFYHVGDPHLVYNMISLLWKGTQLETSMGSIEFASMVGALLIMSQGITLLL 120
           +DLKRFLLSPFYHV D HLVYNM+SLLWKG QLETS+GS+EFASM+ ALL MSQGITLLL
Sbjct: 71  RDLKRFLLSPFYHVSDSHLVYNMMSLLWKGIQLETSVGSVEFASMLAALLAMSQGITLLL 130

Query: 121 IKSLLIFFNYGRPYYYEYSVGFSGVLFAMKVVLGSETDSYTYVYGLRVPSSHAAWLELIL 180
            +SLL+FF+Y +P+Y EYS GFSGVLFAMKVVL S+++S T VYGL +P+ HAAW ELIL
Sbjct: 131 ARSLLLFFDYDKPFYSEYSAGFSGVLFAMKVVLNSQSESLTNVYGLVIPARHAAWAELIL 190

Query: 181 IQMVSPGVSFLGHLGGILAGLLFLRLKGTNSGSDPLTLLIRSVGRILSRSYGFFCRLNPF 240
           IQM  PGVSFLGHLGGILAG+L+++LK   SG DPLTL IR++  ++S    F   L  F
Sbjct: 191 IQMFVPGVSFLGHLGGILAGILYVKLKRAYSGPDPLTLTIRTLTNVISWPLRFVRNLFQF 250

Query: 241 RQRRIFGGGTVGSRPV--TSIGTWRCQACTFDNSGLLSACEMCGTDRQDGVLHSNQLSSQ 300
           R+ RI G G+VG R       G WRCQACT+DNS LLS CEMCGT R    L S + S  
Sbjct: 251 RRGRISGRGSVGGRQTGRAMSGLWRCQACTYDNSSLLSVCEMCGTSRGARGLSSREFSRH 310

Query: 301 L-DLTWEEMRRRRLERFG 315
             DLT EE+R RR+ERFG
Sbjct: 311 SDDLTLEEIRLRRIERFG 328

BLAST of CSPI04G20320 vs. TrEMBL
Match: A0A0A7E6S4_9AQUA (Rhomboid protein Ilepu_RBL14 OS=Ilex purpurea PE=2 SV=1)

HSP 1 Score: 394.4 bits (1012), Expect = 1.3e-106
Identity = 208/317 (65.62%), Postives = 247/317 (77.92%), Query Frame = 1

Query: 1   MLPLLAYQVVSQYYRLQGNP-ITLGLLASNTLIHLRPNFLHHIIPSIDEVWFNAHLIFKH 60
           MLPLLA   VS+Y RL   P +T GLLA+NTLI+LRP+FL  I+P++DEVWFN HLI KH
Sbjct: 13  MLPLLALHAVSEYCRLDRKPPVTAGLLAANTLIYLRPSFLESILPTLDEVWFNPHLILKH 72

Query: 61  KDLKRFLLSPFYHVGDPHLVYNMISLLWKGTQLETSMGSIEFASMVGALLIMSQGITLLL 120
           +DLKRF LS FYH+G+ HLVYNM+SLLWKG QLETSMGS++FASMV ALL MSQGITLLL
Sbjct: 73  RDLKRFFLSAFYHMGESHLVYNMMSLLWKGIQLETSMGSVKFASMVAALLGMSQGITLLL 132

Query: 121 IKSLLIFFNYGRPYYYEYSVGFSGVLFAMKVVLGSETDSYTYVYGLRVPSSHAAWLELIL 180
            KSLL+ F++ R YY+EYSVGFSGVLFAMKV+L S++D YT V+GL +PS HAAW ELIL
Sbjct: 133 AKSLLLLFDHERAYYHEYSVGFSGVLFAMKVILNSQSDDYTSVHGLVLPSRHAAWAELIL 192

Query: 181 IQMVSPGVSFLGHLGGILAGLLFLRLKGTNSGSDPLTLLIRSVGRILSRSYGFFCRLNPF 240
           IQM  PGVSFLGHLGGILAG+L+L LK + SGS+PLT +I    RILS        +  F
Sbjct: 193 IQMFVPGVSFLGHLGGILAGILYLHLKASYSGSNPLTTIIGRFARILSWPVRLVWGICRF 252

Query: 241 RQRRIFGGGTV-GSRPVTSIGTWRCQACTFDNSGLLSACEMCGTDRQDGVLHSNQLSSQL 300
           +Q RIFG GTV GS+  ++ G WRCQACTFDNSG L+ CEMCGT R D  L S   S+Q 
Sbjct: 253 QQPRIFGRGTVGGSQTRSTTGVWRCQACTFDNSGWLNVCEMCGTGRFDDGLSSLGSSNQT 312

Query: 301 -DLTWEEMRRRRLERFG 315
            DL  EE+R+RR++RFG
Sbjct: 313 HDLPVEELRQRRVQRFG 329

BLAST of CSPI04G20320 vs. TrEMBL
Match: A5BGK5_VITVI (Putative uncharacterized protein OS=Vitis vinifera GN=VIT_05s0020g01120 PE=4 SV=1)

HSP 1 Score: 387.5 bits (994), Expect = 1.6e-104
Identity = 208/323 (64.40%), Postives = 246/323 (76.16%), Query Frame = 1

Query: 1   MLPLLAYQVVSQYYRLQGNP-ITLGLLASNTLIHLRPNFLHHIIPSIDEVWFNAHLIFKH 60
           MLPLLA    ++YYR+   P +T GLLA+NTLI+LRP+F+  ++P+ID+VWFN HLI KH
Sbjct: 12  MLPLLALHTANEYYRVGWKPPVTAGLLAANTLIYLRPSFIDSMLPTIDKVWFNPHLILKH 71

Query: 61  KDLKRFLLSPFYHVGDPHLVYNMISLLWKGTQLETSMGSIEFASMVGALLIMSQGITLLL 120
           KDLKRFLLSPFYH+ + HLVYNM+SLLWKG QLETSMGS EFASMV  LL MSQGITLLL
Sbjct: 72  KDLKRFLLSPFYHMDEAHLVYNMMSLLWKGIQLETSMGSAEFASMVATLLGMSQGITLLL 131

Query: 121 IKSLLIFFNYGRPYYYEYSVGFSGVLFAMKVVLGSETDSYTYVYGLRVPSSHAAWLELIL 180
            KSLL+FF+Y R YY +++VGFSGVLFAMKVVL S++  YTYV+GL VP+ HAAW+ELIL
Sbjct: 132 AKSLLLFFDYERAYYSQFAVGFSGVLFAMKVVLNSQSADYTYVHGLIVPTRHAAWVELIL 191

Query: 181 IQMVSPGVSFLGHLGGILAGLLFLRLKGTNSGSDPLTLLIRSVGRILSRSYGFFCRLNPF 240
           IQM  PGVSFLGHLGGILAG+L+LRLKGT SGSDPLT+L + +   LS    F   L  F
Sbjct: 192 IQMFVPGVSFLGHLGGILAGILYLRLKGTYSGSDPLTILFKGLADALSWPLRFAQGL--F 251

Query: 241 RQRRIFGGGTVGSRPV-TSIGTWRCQACTFDNSGLLSACEMCGTDRQDGVLHSNQLSSQL 300
           R+RRI G GTVG R    + G WRC +CT+DN G LS CEMCGT R     + N LSS L
Sbjct: 252 RRRRISGRGTVGGRQTRRTSGVWRCWSCTYDNPGGLSICEMCGTAR-----NGNGLSSSL 311

Query: 301 -------DLTWEEMRRRRLERFG 315
                  +L  +E+RRRRLERFG
Sbjct: 312 QWPRQSHELPLDELRRRRLERFG 327

BLAST of CSPI04G20320 vs. TAIR10
Match: AT3G17611.1 (AT3G17611.1 RHOMBOID-like protein 14)

HSP 1 Score: 373.6 bits (958), Expect = 1.2e-103
Identity = 193/320 (60.31%), Postives = 237/320 (74.06%), Query Frame = 1

Query: 1   MLPLLAYQVVSQYYRLQGNP-ITLGLLASNTLIHLRPNFLHHIIPSIDEVWFNAHLIFKH 60
           MLPLLA   V++YYRL   P +T  LLA+NTL++LRP F+  +IP I EVWFN HLIFKH
Sbjct: 14  MLPLLALSAVAEYYRLPWKPPVTASLLAANTLVYLRPAFIDPVIPHISEVWFNPHLIFKH 73

Query: 61  KDLKRFLLSPFYHVGDPHLVYNMISLLWKGTQLETSMGSIEFASMVGALLIMSQGITLLL 120
           KDLKR  LS FYHV +PHLVYNM+SLLWKG +LETSMGS EFASMV  L+ MSQG+TLLL
Sbjct: 74  KDLKRLFLSAFYHVNEPHLVYNMMSLLWKGIKLETSMGSSEFASMVFTLIGMSQGVTLLL 133

Query: 121 IKSLLIFFNYGRPYYYEYSVGFSGVLFAMKVVLGSETDSYTYVYGLRVPSSHAAWLELIL 180
            KSLL+ F+Y R YY EY+VGFSGVLFAMKVVL S+ + Y+ VYG+ VP+ +AAW ELIL
Sbjct: 134 AKSLLLLFDYDRAYYNEYAVGFSGVLFAMKVVLNSQAEDYSSVYGILVPTKYAAWAELIL 193

Query: 181 IQMVSPGVSFLGHLGGILAGLLFLRLKGTNSGSDPLTLLIRSVGRILSRSYGFFCRLNPF 240
           +QM  P  SFLGHLGGILAG+++L+LKG+ SGSDP+T+ +R V R+++    F   +   
Sbjct: 194 VQMFVPNASFLGHLGGILAGIIYLKLKGSYSGSDPVTMAVRGVSRLVTWPLRFLNGMVRS 253

Query: 241 RQRRIFGGGTV--GSRPVTSIGTWRCQACTFDNSGLLSACEMCGTDRQDG----VLHSNQ 300
           R+RRI G G V  G   +   G WRCQ+CT+DNSG LSACEMCG+ R  G    +     
Sbjct: 254 RRRRITGRGRVGRGQTGIAGPGIWRCQSCTYDNSGWLSACEMCGSGRARGNGWSLNQGPA 313

Query: 301 LSSQLDLTWEEMRRRRLERF 314
           LSS  DL  +E+RRRR+ERF
Sbjct: 314 LSSSNDLPLDELRRRRVERF 333

BLAST of CSPI04G20320 vs. TAIR10
Match: AT3G58460.2 (AT3G58460.2 RHOMBOID-like protein 15)

HSP 1 Score: 73.2 bits (178), Expect = 3.3e-13
Identity = 55/162 (33.95%), Postives = 87/162 (53.70%), Query Frame = 1

Query: 48  EVWFNAHLIFKHKDLKRFLLSPFYHVGDPHLVYNMISLLWKGTQLETSMGSIEFASMVGA 107
           EV F    I     + RF  +  +H    H+++NM++L+  G++LE  MGS+    +   
Sbjct: 73  EVCFLPSAIISRFQVYRFYTAIIFHGSLLHVLFNMMALVPMGSELERIMGSVRLLYLT-V 132

Query: 108 LLIMSQGITLLLIKSLLIFFNYGRPYYYEY-----SVGFSGVLFAMKVVLGSETD-SYTY 167
           LL  +  +  LLI SL     Y   Y Y++     ++GFSG+LF+M V+  S +  +   
Sbjct: 133 LLATTNAVLHLLIASLA---GYNPFYQYDHLMNECAIGFSGILFSMIVIETSLSGVTSRS 192

Query: 168 VYGL-RVPSSHAAWLELILIQMVSPGVSFLGHLGGILAGLLF 203
           V+GL  VP+    W+ LI+ Q++   VS LGHL GIL+G  +
Sbjct: 193 VFGLFNVPAKLYPWILLIVFQLLMTNVSLLGHLCGILSGFSY 230

BLAST of CSPI04G20320 vs. NCBI nr
Match: gi|449457305|ref|XP_004146389.1| (PREDICTED: uncharacterized protein At3g17611 [Cucumis sativus])

HSP 1 Score: 630.2 bits (1624), Expect = 2.0e-177
Identity = 313/314 (99.68%), Postives = 314/314 (100.00%), Query Frame = 1

Query: 1   MLPLLAYQVVSQYYRLQGNPITLGLLASNTLIHLRPNFLHHIIPSIDEVWFNAHLIFKHK 60
           MLPLLAYQVVSQYYRLQGNPITLGLLASNTLIHLRPNFLHHIIPSIDEVWFNAHLIFKHK
Sbjct: 1   MLPLLAYQVVSQYYRLQGNPITLGLLASNTLIHLRPNFLHHIIPSIDEVWFNAHLIFKHK 60

Query: 61  DLKRFLLSPFYHVGDPHLVYNMISLLWKGTQLETSMGSIEFASMVGALLIMSQGITLLLI 120
           DLKRFLLSPFYHVGDPHLVYNMISLLWKGTQLETSMGSIEFASMVGALLIMSQGITLLLI
Sbjct: 61  DLKRFLLSPFYHVGDPHLVYNMISLLWKGTQLETSMGSIEFASMVGALLIMSQGITLLLI 120

Query: 121 KSLLIFFNYGRPYYYEYSVGFSGVLFAMKVVLGSETDSYTYVYGLRVPSSHAAWLELILI 180
           KSLLIFFNYGRPYYYEYSVGFSGVLFAMKVVLGSETDSYTYV+GLRVPSSHAAWLELILI
Sbjct: 121 KSLLIFFNYGRPYYYEYSVGFSGVLFAMKVVLGSETDSYTYVHGLRVPSSHAAWLELILI 180

Query: 181 QMVSPGVSFLGHLGGILAGLLFLRLKGTNSGSDPLTLLIRSVGRILSRSYGFFCRLNPFR 240
           QMVSPGVSFLGHLGGILAGLLFLRLKGTNSGSDPLTLLIRSVGRILSRSYGFFCRLNPFR
Sbjct: 181 QMVSPGVSFLGHLGGILAGLLFLRLKGTNSGSDPLTLLIRSVGRILSRSYGFFCRLNPFR 240

Query: 241 QRRIFGGGTVGSRPVTSIGTWRCQACTFDNSGLLSACEMCGTDRQDGVLHSNQLSSQLDL 300
           QRRIFGGGTVGSRPVTSIGTWRCQACTFDNSGLLSACEMCGTDRQDGVLHSNQLSSQLDL
Sbjct: 241 QRRIFGGGTVGSRPVTSIGTWRCQACTFDNSGLLSACEMCGTDRQDGVLHSNQLSSQLDL 300

Query: 301 TWEEMRRRRLERFG 315
           TWEEMRRRRLERFG
Sbjct: 301 TWEEMRRRRLERFG 314

BLAST of CSPI04G20320 vs. NCBI nr
Match: gi|659082947|ref|XP_008442110.1| (PREDICTED: uncharacterized protein At3g17611 [Cucumis melo])

HSP 1 Score: 605.5 bits (1560), Expect = 5.2e-170
Identity = 301/314 (95.86%), Postives = 307/314 (97.77%), Query Frame = 1

Query: 1   MLPLLAYQVVSQYYRLQGNPITLGLLASNTLIHLRPNFLHHIIPSIDEVWFNAHLIFKHK 60
           MLPLLAYQVVSQYYRLQGNPITLGLLASNTLIHLRPNFLHHIIPSIDEVWFNAHLI KHK
Sbjct: 1   MLPLLAYQVVSQYYRLQGNPITLGLLASNTLIHLRPNFLHHIIPSIDEVWFNAHLILKHK 60

Query: 61  DLKRFLLSPFYHVGDPHLVYNMISLLWKGTQLETSMGSIEFASMVGALLIMSQGITLLLI 120
           DLKRFLLSPFYHVGDPHLVYNMISLLWKGTQLETSMGSIEFASMVGALL+MSQGITLLLI
Sbjct: 61  DLKRFLLSPFYHVGDPHLVYNMISLLWKGTQLETSMGSIEFASMVGALLVMSQGITLLLI 120

Query: 121 KSLLIFFNYGRPYYYEYSVGFSGVLFAMKVVLGSETDSYTYVYGLRVPSSHAAWLELILI 180
           KSLLIFFNYGRPYYYEYSVGFSGVLFA+KVVLGSET+SYTY++GLRVPSSHAAWLELILI
Sbjct: 121 KSLLIFFNYGRPYYYEYSVGFSGVLFALKVVLGSETESYTYIHGLRVPSSHAAWLELILI 180

Query: 181 QMVSPGVSFLGHLGGILAGLLFLRLKGTNSGSDPLTLLIRSVGRILSRSYGFFCRLNPFR 240
           QMVSPGVSFLGHLGGILAGLLFLRLKGTNSGSDPLTLLIRSVGRILSR  GFF RLNPFR
Sbjct: 181 QMVSPGVSFLGHLGGILAGLLFLRLKGTNSGSDPLTLLIRSVGRILSRPLGFFSRLNPFR 240

Query: 241 QRRIFGGGTVGSRPVTSIGTWRCQACTFDNSGLLSACEMCGTDRQDGVLHSNQLSSQLDL 300
           QRRIFGGGTVGSRPVTSIGTWRC+ACTFDNSGLLS CEMCGTDRQDGVLHSNQLSSQLD 
Sbjct: 241 QRRIFGGGTVGSRPVTSIGTWRCRACTFDNSGLLSTCEMCGTDRQDGVLHSNQLSSQLDF 300

Query: 301 TWEEMRRRRLERFG 315
           T EEMRRRRLERFG
Sbjct: 301 TREEMRRRRLERFG 314

BLAST of CSPI04G20320 vs. NCBI nr
Match: gi|802706995|ref|XP_012084232.1| (PREDICTED: uncharacterized protein At3g17611 [Jatropha curcas])

HSP 1 Score: 415.6 bits (1067), Expect = 7.6e-113
Identity = 214/320 (66.88%), Postives = 256/320 (80.00%), Query Frame = 1

Query: 1   MLPLLAYQVVSQYYRLQGNP-ITLGLLASNTLIHLRPNFLHHIIPSIDEVWFNAHLIFKH 60
           +LPLLA   VS+YYRL   P +T GLLA+NTLI+LRP+FLHHI+PSI EVWFN++LI KH
Sbjct: 8   LLPLLAVHAVSEYYRLPWKPPVTAGLLATNTLIYLRPSFLHHILPSIHEVWFNSYLILKH 67

Query: 61  KDLKRFLLSPFYHVGDPHLVYNMISLLWKGTQLETSMGSIEFASMVGALLIMSQGITLLL 120
           +DLKRF LSPFYHVG+ HLVYNMISLLWKG QLE+SMGS EFASMV ALL MSQGITLLL
Sbjct: 68  RDLKRFFLSPFYHVGESHLVYNMISLLWKGIQLESSMGSAEFASMVAALLTMSQGITLLL 127

Query: 121 IKSLLIFFNYGRPYYYEYSVGFSGVLFAMKVVLGSETDSYTYVYGLRVPSSHAAWLELIL 180
            KSLL+FF+YGRP+Y EY+VGFSGVLFAMKVVL S+++ YTYV+GL VP+ HAAW ELIL
Sbjct: 128 SKSLLLFFDYGRPFYSEYAVGFSGVLFAMKVVLNSQSEDYTYVHGLVVPARHAAWAELIL 187

Query: 181 IQMVSPGVSFLGHLGGILAGLLFLRLKGTNSGSDPLTLLIRSVGRILSRSYGFFCRLNPF 240
           +QM  PGVSFLGHLGGILAG+L+L+L+G + G +PLTL+I+++  IL+R   F   L  F
Sbjct: 188 VQMFVPGVSFLGHLGGILAGILYLKLRGMHLGPNPLTLIIKNLAGILARPLRFLRGLFQF 247

Query: 241 RQRRIFGGGTVGS----RPVTSIGTWRCQACTFDNSGLLSACEMCGTDRQDGVLHSNQLS 300
           R+++I G GTVG     R V+ +  WRCQACT+DNSG LS CEMCGT R D    S Q S
Sbjct: 248 RRQQISGRGTVGGSQAGRSVSRV--WRCQACTYDNSGWLSVCEMCGTTRGDNGSSSRQFS 307

Query: 301 SQLD-LTWEEMRRRRLERFG 315
            Q D  T EE+RRRR++RFG
Sbjct: 308 HQFDNPTMEEVRRRRIQRFG 325

BLAST of CSPI04G20320 vs. NCBI nr
Match: gi|743910992|ref|XP_010999348.1| (PREDICTED: uncharacterized protein At3g17611 [Populus euphratica])

HSP 1 Score: 400.6 bits (1028), Expect = 2.5e-108
Identity = 209/318 (65.72%), Postives = 244/318 (76.73%), Query Frame = 1

Query: 1   MLPLLAYQVVSQYYRLQGNP-ITLGLLASNTLIHLRPNFLHHIIPSIDEVWFNAHLIFKH 60
           MLPLLA   VS+YYRL   P +T  LL +NTLI+LRP FL HI+PSID+VWFN HLI K+
Sbjct: 11  MLPLLAIHAVSEYYRLPWKPPVTAALLGANTLIYLRPAFLRHILPSIDQVWFNPHLILKY 70

Query: 61  KDLKRFLLSPFYHVGDPHLVYNMISLLWKGTQLETSMGSIEFASMVGALLIMSQGITLLL 120
           +DLKRFLLSPFYHV D HLVYNM+SLLWKG QLETS+GS+EFASM+ ALL MSQGITLLL
Sbjct: 71  RDLKRFLLSPFYHVSDSHLVYNMMSLLWKGIQLETSVGSVEFASMLAALLAMSQGITLLL 130

Query: 121 IKSLLIFFNYGRPYYYEYSVGFSGVLFAMKVVLGSETDSYTYVYGLRVPSSHAAWLELIL 180
            KSLL+FF+Y +P+Y EYS GFSGVLFAMKVVL S+++S T VYGL +P+ HAAW ELIL
Sbjct: 131 AKSLLLFFDYDKPFYSEYSAGFSGVLFAMKVVLNSQSESLTNVYGLVIPARHAAWAELIL 190

Query: 181 IQMVSPGVSFLGHLGGILAGLLFLRLKGTNSGSDPLTLLIRSVGRILSRSYGFFCRLNPF 240
           IQM  PGVSFLGHLGGILAG+L+L+LK   SG DPLTL IR++  ++S    F   L  F
Sbjct: 191 IQMFVPGVSFLGHLGGILAGILYLKLKRAYSGPDPLTLTIRTLTNVISWPLRFVRNLFQF 250

Query: 241 RQRRIFGGGTVGSRPV--TSIGTWRCQACTFDNSGLLSACEMCGTDRQDGVLHSNQLSSQ 300
           R+ RI G G+VG R    T  G WRCQACT+DNS +LS CEMCGT R    L S + S  
Sbjct: 251 RRGRISGRGSVGGRQTGRTMSGLWRCQACTYDNSSMLSVCEMCGTSRGARGLSSREFSRH 310

Query: 301 L-DLTWEEMRRRRLERFG 315
             DLT EE+R RR+ERFG
Sbjct: 311 SDDLTLEEIRLRRIERFG 328

BLAST of CSPI04G20320 vs. NCBI nr
Match: gi|1009179812|ref|XP_015871286.1| (PREDICTED: rhomboid-like protein 14, mitochondrial [Ziziphus jujuba])

HSP 1 Score: 399.4 bits (1025), Expect = 5.7e-108
Identity = 212/317 (66.88%), Postives = 243/317 (76.66%), Query Frame = 1

Query: 1   MLPLLAYQVVSQYYRLQGNP-ITLGLLASNTLIHLRPNFLHHIIPSIDEVWFNAHLIFKH 60
           MLPLLA    S+YYRL+  P +T GL+A+NTLI+LRP FL  +IPSIDEVWFN HLI K+
Sbjct: 11  MLPLLAIHAFSEYYRLERKPPVTAGLVAANTLIYLRPAFLDSLIPSIDEVWFNPHLILKN 70

Query: 61  KDLKRFLLSPFYHVGDPHLVYNMISLLWKGTQLETSMGSIEFASMVGALLIMSQGITLLL 120
           KDLKR  LS FYHV D HLVYNM+SLLWKG QLE SMGS+EFASM+ ALL MSQGITLLL
Sbjct: 71  KDLKRLFLSAFYHVSDSHLVYNMMSLLWKGIQLENSMGSVEFASMIVALLGMSQGITLLL 130

Query: 121 IKSLLIFFNYGRPYYYEYSVGFSGVLFAMKVVLGSETDSYTYVYGLRVPSSHAAWLELIL 180
            KSLL+FF+Y R YY EY+VGFSG+LFAMKVVL S +DS T VYGL VP+ HAAW ELIL
Sbjct: 131 AKSLLLFFDYDRAYYSEYAVGFSGILFAMKVVLNSRSDSLTQVYGLIVPARHAAWAELIL 190

Query: 181 IQMVSPGVSFLGHLGGILAGLLFLRLKGTNSGSDPLTLLIRSVGRILSRSYGFFCRLNPF 240
           IQ+  PGVSFLGHLGGILAG+L+LRLKG+ SGSDPLTLLIR   ++LS    F   L   
Sbjct: 191 IQLFVPGVSFLGHLGGILAGILYLRLKGSFSGSDPLTLLIRGFTKVLSWPLRFVKELFRL 250

Query: 241 RQRRIFGGGTVGS--RPVTSIGTWRCQACTFDNSGLLSACEMCGTDRQDGVLHSNQLSSQ 300
           R+RRI G GTVG   R     G WRC ACT+DN+G LS CEMCGT + D  L S+Q S  
Sbjct: 251 RRRRISGRGTVGGGHRGRIEAGVWRCDACTYDNAGWLSVCEMCGTPQSDSGLLSHQFSPH 310

Query: 301 -LDLTWEEMRRRRLERF 314
             DL+ EE+RRRR+ERF
Sbjct: 311 PQDLSLEELRRRRIERF 327

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
RBL14_ARATH2.1e-10260.31Rhomboid-like protein 14, mitochondrial OS=Arabidopsis thaliana GN=RBL14 PE=2 SV... [more]
RHBL4_MOUSE2.4e-2631.96Rhomboid-related protein 4 OS=Mus musculus GN=Rhbdd1 PE=1 SV=1[more]
RHBL4_HUMAN6.0e-2534.62Rhomboid-related protein 4 OS=Homo sapiens GN=RHBDD1 PE=1 SV=1[more]
RHBL4_RAT5.6e-2335.68Rhomboid-related protein 4 OS=Rattus norvegicus GN=Rhbdd1 PE=2 SV=1[more]
RHBL4_PONAB2.8e-2236.22Rhomboid-related protein 4 OS=Pongo abelii GN=RHBDD1 PE=2 SV=1[more]
Match NameE-valueIdentityDescription
A0A0A0L173_CUCSA1.4e-17799.68Uncharacterized protein OS=Cucumis sativus GN=Csa_4G506850 PE=4 SV=1[more]
A0A067JVV7_JATCU5.3e-11366.88Uncharacterized protein OS=Jatropha curcas GN=JCGZ_19107 PE=4 SV=1[more]
B9HXC8_POPTR2.0e-10765.09Rhomboid family protein OS=Populus trichocarpa GN=POPTR_0010s00720g PE=4 SV=1[more]
A0A0A7E6S4_9AQUA1.3e-10665.62Rhomboid protein Ilepu_RBL14 OS=Ilex purpurea PE=2 SV=1[more]
A5BGK5_VITVI1.6e-10464.40Putative uncharacterized protein OS=Vitis vinifera GN=VIT_05s0020g01120 PE=4 SV=... [more]
Match NameE-valueIdentityDescription
AT3G17611.11.2e-10360.31 RHOMBOID-like protein 14[more]
AT3G58460.23.3e-1333.95 RHOMBOID-like protein 15[more]
Match NameE-valueIdentityDescription
gi|449457305|ref|XP_004146389.1|2.0e-17799.68PREDICTED: uncharacterized protein At3g17611 [Cucumis sativus][more]
gi|659082947|ref|XP_008442110.1|5.2e-17095.86PREDICTED: uncharacterized protein At3g17611 [Cucumis melo][more]
gi|802706995|ref|XP_012084232.1|7.6e-11366.88PREDICTED: uncharacterized protein At3g17611 [Jatropha curcas][more]
gi|743910992|ref|XP_010999348.1|2.5e-10865.72PREDICTED: uncharacterized protein At3g17611 [Populus euphratica][more]
gi|1009179812|ref|XP_015871286.1|5.7e-10866.88PREDICTED: rhomboid-like protein 14, mitochondrial [Ziziphus jujuba][more]
The following terms have been associated with this gene:
Vocabulary: INTERPRO
TermDefinition
IPR001876Znf_RanBP2
IPR022764Peptidase_S54_rhomboid_dom
IPR001876Znf_RanBP2
IPR022764Peptidase_S54_rhomboid_dom
IPR001876Znf_RanBP2
IPR022764Peptidase_S54_rhomboid_dom
Vocabulary: Molecular Function
TermDefinition
GO:0008270zinc ion binding
GO:0004252serine-type endopeptidase activity
GO:0008270zinc ion binding
GO:0004252serine-type endopeptidase activity
GO:0008270zinc ion binding
GO:0004252serine-type endopeptidase activity
Vocabulary: Cellular Component
TermDefinition
GO:0016021integral component of membrane
GO:0016021integral component of membrane
GO:0016021integral component of membrane
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0006508 proteolysis
cellular_component GO:0016021 integral component of membrane
molecular_function GO:0004252 serine-type endopeptidase activity
molecular_function GO:0008270 zinc ion binding
molecular_function GO:0046872 metal ion binding

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
CSPI04G20320.2CSPI04G20320.2mRNA
CSPI04G20320.1CSPI04G20320.1mRNA
CSPI04G20320.3CSPI04G20320.3mRNA


Analysis Name: InterPro Annotations of cucumber (PI183967)
Date Performed: 2017-01-17
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR001876Zinc finger, RanBP2-typePFAMPF00641zf-RanBPcoord: 258..286
score: 5.
IPR001876Zinc finger, RanBP2-typeSMARTSM00547zf_4coord: 259..283
score: 4.
IPR001876Zinc finger, RanBP2-typePROSITEPS01358ZF_RANBP2_1coord: 261..280
scor
IPR001876Zinc finger, RanBP2-typePROFILEPS50199ZF_RANBP2_2coord: 257..286
score: 8
IPR001876Zinc finger, RanBP2-typeunknownSSF90209Ran binding protein zinc finger-likecoord: 260..284
score: 6.0
IPR022764Peptidase S54, rhomboid domainGENE3DG3DSA:1.20.1540.10coord: 20..203
score: 2.5
IPR022764Peptidase S54, rhomboid domainPFAMPF01694Rhomboidcoord: 58..205
score: 2.1
NoneNo IPR availablePANTHERPTHR12917ASPARTYL PROTEASE DDI-RELATEDcoord: 1..313
score: 4.9
NoneNo IPR availablePANTHERPTHR12917:SF18RHOMBOID DOMAIN-CONTAINING PROTEIN 3coord: 1..313
score: 4.9
NoneNo IPR availableunknownSSF144091Rhomboid-likecoord: 19..204
score: 1.44