Lsi01G017460 (gene) Bottle gourd (USVL1VR-Ls)

NameLsi01G017460
Typegene
OrganismLagenaria siceraria (Bottle gourd (USVL1VR-Ls))
DescriptionDNA-directed RNA polymerase II subunit RPB4
Locationchr01 : 16266677 .. 16274242 (+)
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: CDSthree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGGAATATCGAATCTCTCGATACTCAACTATGGTGGTAGAAGTAGATATAGATCTTCCAACGTCGAAAAAAATCCCAAAGCATCCATCACCAATCGAACTAATTGGCCTGATCTTCACCCTCCGTCGTCGTTGCAACCACTTCCTAGTATCGGAGAATGGCGAACGACAATTAGTAGAGCATAAAGAAATACCAAAAGTTCCACTATGCGACCACCAATTCCCTGCTGAAATGTTCAATGGAGTGGACAATTCTCTATTCCATTACCTTTCTCATAGTTTCGAAAACTACAAACCCAAAATTGATGCAGAGCTAGTCGCTGATGCCCTAATCGAGCGGTGGATTGAAATAAGGGACCAGGTGAATTCAAGAAGCTCCGACAAAGTGTTTTCAATGACGGTAAATTTGACGATTTCGAATGTCGTGGCTCGACATGAAATTGGTAGTGACAGAAACGTATTCGGTGGAGAATATTTTAGGATCGTTCCAGCATGTGGCGAAGTAATTAGGAAAACGTTGAAGAGATTGGAGATGAAAGAAGAAGATGAAAGAATTTGTGTGATATGTTTGGATGAATTGAAGAAAAAGAGGAATGGAAGGTGTGGTTTGGAAATGCCTTGTTCACATGTGTTTCATGGGAAATGCATTGAAGAGTGGTTGAATAATAGCCATTGTTGTCCAATTTGTAGATTTGAAATGCCTATCCATTCAATATGAACATTTTTGAAAATGTATTTGAAAATATATTTATAAACTATAAGACTAATTATAGTATTATGGTTACTCAGTAAATACTAAGTTGAATATAAAATATTACTAATTAATTTATCAACTTGTTTTATCTTAAATTTTTTATATAATTGTCATTTTGTAATATCATATAATATAATATATTACATATTACATATTTTAATTTAACAAAAATAATTGAGTTTATAACTTGAAATACTATACTTGTTATTGTTTTTTAAAATTTTGAATTCAAAATATTTACACAATTTGAATCATAAAATTTGAAAACATAATTTAAATGGTCAACCAAATGCATATTTACTTAACTTAATAAATCTAAAAACATAAAATAGATTTTGGGTTGGATAATAAATGTGCCATAATTAATTATGATTTTTCTTTCTTTCCTCTTACCCATAAATGTGGATTTTGGTTTCAGAATGTTCTATTATTAAAAGAAAAAGGAAAATTTTATTTTGGTACAAATTAAGTATTTTCCTTCTCATTATTAGTGAGTATTTATTTGGGTTCTATCCGTAAAATTTTTATATATTTATAAATAGTTTGAAATTTTTTTATTTATTTAATTTATTGAATTGTGATAAAATTATGTTCAACAATTAGTGGATAGCATTTGATAAATTTTGTGAATTTTCTTGAATTCTAAGATTAGAAATATTAGATTGTCACAAGCATGTGTAACATGCTTTTCTTTATTTCTTTATGTGTAGTATAGTTTAGTTTTACTCGCCATAGTGGATTTTGTTTTAATTTCGTTTCCTTCTTATGTGATGTAATTCTATGAAAGAAAAAAAAACAAACAAAAAAAGGAAAAATTTCCAACGATATAATTTAATTATAGGATGTTAAATTAAATCTAGGGACGTCATGGTTAAATTGCAAATTTGATCTCTACTAGTCGAAACTACTGCGTTGCATGTGTTTTATAAAGTTGAAAATATATGTATAAGTTGAAATAAAAGTCTTTTCATTTTTTCTTTATATTTAAAAAAAAGTTCATACCACTCAAAACAAATTTGAATTAATATATATTATTTAAACTGTTTTTAAAATTTTTGAATTGATTTTTTTGAATTCTTAGTTCAAATTTTTGATAATAAGAGAATAAACAAATAATCTTGACATTTATAATAAGAAAATTATAAAATAAAAGCATCACATCGATTTTTTTTAGATAAAATGATATCAGAGTTCTCTTTTTAATTTTTTTAACGTCAATTATTATTTTTTTGAGATAAAGTGAGATTTTCATTTATGATTTTTTATATAACATGAGATTTTTTTTATAAAGAAATATTTTTTTAATATGAATTATTCTTAGATAAAATCAATTTTTTTAAAAGAAAAATTAGGGAAAATTACCTTTTTAGTTCTCAAGTTTTGAACAATATGTTCGTTTGGTCCATGAGTTAACAAACGTACCTTTTTAGTTTCTGAGTTTATAAGAATAGGTCCATTTGGTTTCTGAGTTTTCAAAATATACATTTTCAGTCTCTAAATTTTTAAAAATAGGTCTAAAAGGTCTTCCAAGTATTTTTTTCTTTGATTTTTTAAAAATAATTATATGATATTTAAAATTAGAAAAAATAATTTTAGAGACGAAATGGTTTTTAAAAATTATTCTTCTAATTTAAAATGTCATATAATTATTTAAATTAATAATAAAAAAATATTTGAGGGATGTCTTAAACCTATTTTTAACAATTCAGTGACTAAGAAGTTATATTTTAAAAACATAGGGACCAAATGAACCTATTCTTATAAACTCGGAGACTAAAAATGTATATTTTAAAAACTCAGAGACCAAATGCACATATTGTTTAAAACTTGGGGACTAAAAAGATAATCTTCCAAAAAAAAAAAAAAATTATTATCACCCCCAACTTTCTCAATTCAATGTGAATGCGAGAAAATTTTATAAAACAATTTAAATATAAAATTACTTAAGATATTATTATAACCAATCACTATTATTATTTTTAATCTTAGTTTGACATATTTTTGAAAGAAGTCTTTTCACTTAATGCTTGTTTAAATACAAAATAGTTCAAGAAAAGGGATTAGAAAAGAAAAAGAGAAGAAAAGGGTTTGGTCCCTCAACTCTCTCAGATATCTCTTTCTCCCCCTCTTCTCCTCCTCTTCTTCCGCCCCTTCAGTCGCAGTCCACCACAGCCGCCCGCCGATTCCCTGTCGCCGGTAAGTCGCCGTTCGCCTCTCTTTTTCTTTCTCTTCTCTCTCGCTCTCTCGGTTTTCACTCTTCTTCTCACGCGCAGATTCCACTGCTTTTCCGCTTTTCTGTCGCCTTTCGATCAACGCCGCAACCGACCGGACCACCCACGCCCAAAAGAGAAATAATTTGTGGGAATCAATCGATTGAGGTAAGGAATAGAGGACCCTTTTGATTAATTTTTGGCTATGTTTGATAACTTCAATGTATCATTAGATTAATATAAAATTGATTTGGGCTTCTGATGCTTCCTAATTTTGTAGGATTAAATTTTGATTTATTAGTCCTAGTCCATTTAAGCAAAGCTATTTTTTATGTTGTAGATCTAGTTGTGATCTTGCTGTACTCTGTTTCCAGATTCTTTAGGTTGACACAAAACTCCAACTTCAAGCATGTCTGGAGTTCAAGAGGAAGAAAATGCCGCAGAGCTTAAGATTGGGGATGGTAAAAATCTATCTATAAATTTCTAGATTCTGCAAGCATGTCTGTTTCCAGATTCTTTAGGTTGACACAAAATCTATCTATATTCAATGAGATTTTAAGTTTCTTAGATGGGGTTCCTCCATATATTTGTAAAATTAGAAACTTAATTTATTATGTTGATCAATGTTCCATGCTTAATTAAATGCATTTTTTTTTTTTATCAATAATCTTTGAACCTTCTAGTAATCAGTGTGAGATTGTTCTTATCTTTTTAATAGAGCATATGATTTGTATTGTTAGGCATGACATAGTGGTTAGATATAGTGCCTTGTAGGTGGCTGGACACTTTTAGTTTCTTTTGTATAGACATCAAATCCTTATTTAATATGTTGTTTAGGCCCAAACGACTAAATGCTTTTGATTTTTCTTATATTTTGGTCCTTTGGATTAATATTGTATGGTTATTAGGTTTCTTAAGTTTTAGATGAAGATGTGTGATCTATGCAAAATTTAAATTCTAATCTATAATTGAGCTGTATAACTGTTTGTAAGCTTTTTCAGTTTTTATGAATCTATGATAATACAAGGCTTTAGAATGGTCAAATCTTTGTTTTACTAATTGGGCAAAATTTAAGCTTGTGCCACCTAGATCATTTTGGTGTATATTCTAAGCTTTTTCAGATAGTAACTTATATTTACTTTCTTTGGCTACTCGTAGAGTTCTTAAAGGCCAAATGCTTAATGAACTGCGAAGTGTCACTAATTTTAGAACACAAATATGAGCAGCTCCAACAAATGTCTGAGGATCCAACAAATCAAGTTTCTCAGTGAGTTTTATGTTTACTCGTTATTGATTCATAAGTCAATTTAGAATCGCTATTGCTTATTGAGATAGCTAATGATAGATTTTTATTTATGGTTTTTTGCAGAGTGTTTGAAAAGTCACTTCATTATGTAAAACGTTTTAGTCGTTATAAGAACCCTGATGCTGTTAGACAAGTCCGAGAGTATCCTTATATAATAATATTTTTCATTTTATGGGATGGTGGTAGGTGGGCATAATTACTATTTTCGTGTTCTTAAAAATCCGTTGAATCTTAACTATAATATAGAATTCTAAGCAGGTATCAGTTGGCAGAGTTTGAGGTATGTATTGATCCATTTGTTTAATCAACTTACTAATTATTATTCTAAAGAAAAGTTTGTCTCATCCAGTTTGAAATTTGCAGCTCTGCGTACTTGGCAATCTTTGTCCTGAAACAGTTGAGGAAGCTATTGCTATGGTACCGTCTTTAAAGGTATTTTGTCATTTTTTTTAACCGGTTGTATATTTATTGGTACTTTGTCTATAAATAGATACAAACTTTTAGAAAATCGACTGTTGTGCTAATATTTCTACATTGAACCTGCATTGAATTTTAAAATACTTGGAAGTTAAAATGTAATGCATCATGTTTTAGAGAATAAAATGTAATTCATTATTATTATTGGTGTTTAAAATTTGGAAAAGATTTCAAATGTGTTTTTTAAGATAAAATAAATTAGACATGGAAAAAACCATTGACGGTAAAAAGGAGACAAAGTCTCAATAATTGGCTAAGAGGTCATGAGTTCAATCCATGGTAGCTACTTACCTAGTAATTAATTTCCTACGAGTTTCCTTGACACCCAAATGTTGTAGGGTCAGGCGGGTTGTTCTGTGAGATTAGTCGAGGTGTGCTGGTCTGGACCCTCACGGATATCAAAAAAAAAAAAAAAAAAAAAAAGAAAATTAGACATGGAATGGAATTTTCTAATGAATCCTTTTGTTGATTTCAGACTAAAGGGAGAGCACATGACGACGAAGCTATTGAGAAGATGTTGAATGACCTTTCATTGATAAAAAGATTTGAGTAGAAGTTTAAAAACACCTAAATTAATCTCAGGGGTAAAAGTCCAAGTCAATTTGTAAGTATTACTTTTCGTTATGGTAATTGTATTCAAGTTTGTTTTTGTGAGACTTTTATTACCGGGCCTTTATATATGGCTGATTTTATCAAATATATATGTATATGGAAGCCTCTATGGAAATTTTCCACTATGCTCATGTTGTAATATGGATGTAAAGTCTACTTACCTGGACAGTAAAATTCAGGAGTTTTCAATATGCTATGAGAAGTTGTGTTATGTTATGAAATGCATGTCATGAAAAGTCATGTAGTATTATGGAAAGTTATGTTAGTCATTTATGTAAATCATGGATGAAAAGTCTATTTACTTTGCACATTTGTGAAATTAATATTTATGGAACCTCGTATGCATATACTTATGAAAATGCTTAATATTTTTCCTGCGCACGAAAGAAGTGTATGTTCTAGAGATTATGATACATGAAAGTGATGTATACTTAGAGAGACATAGAATAAATAAAAGTGATGCACGCTTAGTGAATTCTTTGTATATAGAACTGTGTATGAAAGTGATGCACATCTAAGAAGAAAGTTTAGGTCCAATTACATGTTGGATGTGTGTGTGCACATCCCAAACCCAACGTTAAGGCCTTTTGAGTGTTTTTAATACTCACATCTTTCAAAACCTTCGTTTAAGAAATATACAAGTTGCATGACAAGGTAGTCTCTGACTCTCTTGGGACTAGACTTGTCTTGTTCTGCATTTAGTCATCTCTTTAAATTTATTTAGATGTATATATAAAAAAAATTCAAATTTTGTTTTCCCATTCGAACCATTTCTTTACTTGTTTTAATAGGGTTTGAATTATATTTGTTAAAAATAGTTTTATTATAAAAGTCATGCTTTTAATGATGTTGTTGACTGAGAAATTTCTACCAATTGCGAATTGCCACACATTAATTTAGACTAGTTATTGATAGATTTGAGACAAATTACAAATTTACACGTGTCCTCAAATTACAGGAATGTTAGCTCGTCCAAGCTAGGTCATCTTGGATTTATTAAAATAAATTAGGTCAAACTTAAATAAAAATCTATTAAAAATAGGTCAAACTTAAATAAAAATCTATTAAAAAAAGATTAATTTAAGTTCAAGTTTAAAAGTTAGGCTTGATGTCAACCAAGCCCAAGTGCATATAGGATCTCCTAAAACTCTATAAATAAATAGAGGAGTTTACCCTTCATTTGAGGCATGAAGTCAGAAATTGAAGAAGCCAACACTCATACATCTATTGATCCAGCAAATTGATCGTCTCTTCTCCAACGAATTGATCATCTCTTCTTCAACGAATTGAGCATCTCTTCTTCAACGAATTGATTGTCTATTATTCACCAAATTAATCGTCTCTTCACCATCTATAAATTGAAGACCGATTTCTCCTAATTTATGAATAAGCTCATCAGTCCTCAGATCAAAGTCAATTGATCAACAAACCAGATGTTGATTCAAACTGAACAACAAGACAAAGGCCGATTCAAGTCGATTAATAAGCCAAAGGTTGATCCAAGCTGATCAACAAGCCAAAGACTGATCCAAGCTGATCAACTAGTCAGAGGCCGATCATTCAAGAAGATCAAGAAGTCCACAAGCCGATCCTCCAAGAAGATCATTAAAGCCCAAAGGTCGATCATCCAAGAAGATCAAGAAGTCCAAAGATCAATCCTCCAAGAAGATCATCATAAAAATTAGAGACTGTAACACTTAAACTACAAATCAATAAAATCAAGTTCATTTCCACGAACTAAATTTCCTTTGTCGAAATCTCATGTGAACACATGACCCTAGACAAAGGTCTTAAAAAGTTGGATTGTTATAGTACCACCTCATTTCTATTCATGGTGTTTATTGCACTTTGTAATGAGAATTAATGGTGGGCCTTCTTTTAAAAAATGTAATCATATCATGTAATCCTATTACACCCAAAAAGGTCATCAATCTCATTCTCATTTATTTTTACGTGGGATCCACACTTATGTGCCTTATATGCTTTATATAAGTTCTAATGTCCCCTGGGTGGTTTCATTATTCTTTTGTTTCTTGCTCTAGTCTATATACCTTATATACCTTAATTGAAGAGGGTTGTTTTTTTGTTTTTGTTTTTTTTATTAGATAAATAAAGGTCCAACAGTCAGCATGAAGCAATAGAATCAATAACAAAAAGAGAATCAAGTGACGAGGGATGAAGGAGAGTAAGAGAGTATTGAAAGAGAGC

mRNA sequence

ATGGAATATCGAATCTCTCGATACTCAACTATGGTGGTAGAAGTAGATATAGATCTTCCAACGTCGAAAAAAATCCCAAAGCATCCATCACCAATCGAACTAATTGGCCTGATCTTCACCCTCCGTCGTCGTTGCAACCACTTCCTAGTATCGGAGAATGGCGAACGACAATTAGTAGAGCATAAAGAAATACCAAAAGTTCCACTATGCGACCACCAATTCCCTGCTGAAATGTTCAATGGAGTGGACAATTCTCTATTCCATTACCTTTCTCATAGTTTCGAAAACTACAAACCCAAAATTGATGCAGAGCTAGTCGCTGATGCCCTAATCGAGCGGTGGATTGAAATAAGGGACCAGGTGAATTCAAGAAGCTCCGACAAAGTGTTTTCAATGACGGTAAATTTGACGATTTCGAATGTCGTGGCTCGACATGAAATTGGTAGTGACAGAAACGTATTCGGTGGAGAATATTTTAGGATCGTTCCAGCATGTGGCGAAGTAATTAGGAAAACGTTGAAGAGATTGGAGATGAAAGAAGAAGATGAAAGAATTTGTGTGATATGTTTGGATGAATTGAAGAAAAAGAGGAATGGAAGAGTGTTTGAAAAGTCACTTCATTATGTAAAACGTTTTAGTCGTTATAAGAACCCTGATGCTGTTAGACAAGTCCGAGAGTATCCTTATATAATAATATTTTTCATTTTATGGGATGGTGGTAGGTATCAGTTGGCAGAGTTTGAGCTCTGCGTACTTGGCAATCTTTGTCCTGAAACAGTTGAGGAAGCTATTGCTATGGTACCGTCTTTAAAGACTAAAGGGAGAGCACATGACGACGAAGCTATTGAGAAGATGTTGAATGACCTTTCATTGATAAAAAGATTTGAGTAGAAGTTTAAAAACACCTAAATTAATCTCAGGGGTAAAAGTCCAAGTCAATTTATAAATAAAGGTCCAACAGTCAGCATGAAGCAATAGAATCAATAACAAAAAGAGAATCAAGTGACGAGGGATGAAGGAGAGTAAGAGAGTATTGAAAGAGAGC

Coding sequence (CDS)

ATGGAATATCGAATCTCTCGATACTCAACTATGGTGGTAGAAGTAGATATAGATCTTCCAACGTCGAAAAAAATCCCAAAGCATCCATCACCAATCGAACTAATTGGCCTGATCTTCACCCTCCGTCGTCGTTGCAACCACTTCCTAGTATCGGAGAATGGCGAACGACAATTAGTAGAGCATAAAGAAATACCAAAAGTTCCACTATGCGACCACCAATTCCCTGCTGAAATGTTCAATGGAGTGGACAATTCTCTATTCCATTACCTTTCTCATAGTTTCGAAAACTACAAACCCAAAATTGATGCAGAGCTAGTCGCTGATGCCCTAATCGAGCGGTGGATTGAAATAAGGGACCAGGTGAATTCAAGAAGCTCCGACAAAGTGTTTTCAATGACGGTAAATTTGACGATTTCGAATGTCGTGGCTCGACATGAAATTGGTAGTGACAGAAACGTATTCGGTGGAGAATATTTTAGGATCGTTCCAGCATGTGGCGAAGTAATTAGGAAAACGTTGAAGAGATTGGAGATGAAAGAAGAAGATGAAAGAATTTGTGTGATATGTTTGGATGAATTGAAGAAAAAGAGGAATGGAAGAGTGTTTGAAAAGTCACTTCATTATGTAAAACGTTTTAGTCGTTATAAGAACCCTGATGCTGTTAGACAAGTCCGAGAGTATCCTTATATAATAATATTTTTCATTTTATGGGATGGTGGTAGGTATCAGTTGGCAGAGTTTGAGCTCTGCGTACTTGGCAATCTTTGTCCTGAAACAGTTGAGGAAGCTATTGCTATGGTACCGTCTTTAAAGACTAAAGGGAGAGCACATGACGACGAAGCTATTGAGAAGATGTTGAATGACCTTTCATTGATAAAAAGATTTGAGTAG

Protein sequence

MEYRISRYSTMVVEVDIDLPTSKKIPKHPSPIELIGLIFTLRRRCNHFLVSENGERQLVEHKEIPKVPLCDHQFPAEMFNGVDNSLFHYLSHSFENYKPKIDAELVADALIERWIEIRDQVNSRSSDKVFSMTVNLTISNVVARHEIGSDRNVFGGEYFRIVPACGEVIRKTLKRLEMKEEDERICVICLDELKKKRNGRVFEKSLHYVKRFSRYKNPDAVRQVREYPYIIIFFILWDGGRYQLAEFELCVLGNLCPETVEEAIAMVPSLKTKGRAHDDEAIEKMLNDLSLIKRFE
BLAST of Lsi01G017460 vs. Swiss-Prot
Match: NRPB4_ARATH (DNA-directed RNA polymerase II subunit 4 OS=Arabidopsis thaliana GN=NRPB4 PE=1 SV=1)

HSP 1 Score: 150.6 bits (379), Expect = 2.7e-35
Identity = 79/97 (81.44%), Postives = 83/97 (85.57%), Query Frame = 1

Query: 200 RVFEKSLHYVKRFSRYKNPDAVRQVREYPYIIIFFILWDGGRYQLAEFELCVLGNLCPET 259
           +VFEKSL YVKRFSRYKNPDAVRQVRE        IL    R+QL EFELCVLGNLCPET
Sbjct: 53  QVFEKSLQYVKRFSRYKNPDAVRQVRE--------IL---SRHQLTEFELCVLGNLCPET 112

Query: 260 VEEAIAMVPSLKTKGRAHDDEAIEKMLNDLSLIKRFE 297
           VEEA+AMVPSLKTKGRAHDDEAIEKMLNDLSL+KRFE
Sbjct: 113 VEEAVAMVPSLKTKGRAHDDEAIEKMLNDLSLVKRFE 138

BLAST of Lsi01G017460 vs. Swiss-Prot
Match: RPB4_DICDI (DNA-directed RNA polymerase II subunit rpb4 OS=Dictyostelium discoideum GN=polr2d PE=3 SV=1)

HSP 1 Score: 65.9 bits (159), Expect = 8.7e-10
Identity = 35/96 (36.46%), Postives = 57/96 (59.38%), Query Frame = 1

Query: 200 RVFEKSLHYVKRFSRYKNPDAVRQVREYPYIIIFFILWDGGRYQLAEFELCVLGNLCPET 259
           R F K+L Y ++FSRYKN  +++QVR               +  L EFE+  L NLCPE 
Sbjct: 74  RTFHKTLAYAEKFSRYKNKTSIKQVRTAL-----------SKQNLEEFEIASLANLCPEI 133

Query: 260 VEEAIAMVPSLKTKGRAHDDEAIEKMLNDLSLIKRF 296
            +EA +++PSLK      +D+ ++ +L++LS +++F
Sbjct: 134 SDEAKSLIPSLKKM----EDDELQAILDELSNLRKF 154

BLAST of Lsi01G017460 vs. Swiss-Prot
Match: RPB4_HUMAN (DNA-directed RNA polymerase II subunit RPB4 OS=Homo sapiens GN=POLR2D PE=1 SV=1)

HSP 1 Score: 64.3 bits (155), Expect = 2.5e-09
Identity = 37/96 (38.54%), Postives = 58/96 (60.42%), Query Frame = 1

Query: 201 VFEKSLHYVKRFSRYKNPDAVRQVREYPYIIIFFILWDGGRYQLAEFELCVLGNLCPETV 260
           VF K+L+Y  RFSR+KN + +  VR               + +L +FEL  L NLCPET 
Sbjct: 60  VFMKTLNYTARFSRFKNRETIASVRSLLL-----------QKKLHKFELACLANLCPETA 119

Query: 261 EEAIAMVPSLKTKGRAHDDEAIEKMLNDLSLIKRFE 297
           EE+ A++PSL  +GR  +DE ++++L+D+   + F+
Sbjct: 120 EESKALIPSL--EGR-FEDEELQQILDDIQTKRSFQ 141

BLAST of Lsi01G017460 vs. Swiss-Prot
Match: RPB4_MOUSE (DNA-directed RNA polymerase II subunit RPB4 OS=Mus musculus GN=Polr2d PE=1 SV=2)

HSP 1 Score: 64.3 bits (155), Expect = 2.5e-09
Identity = 37/96 (38.54%), Postives = 58/96 (60.42%), Query Frame = 1

Query: 201 VFEKSLHYVKRFSRYKNPDAVRQVREYPYIIIFFILWDGGRYQLAEFELCVLGNLCPETV 260
           VF K+L+Y  RFSR+KN + +  VR               + +L +FEL  L NLCPET 
Sbjct: 60  VFMKTLNYTARFSRFKNRETIASVRSLLL-----------QKKLHKFELACLANLCPETA 119

Query: 261 EEAIAMVPSLKTKGRAHDDEAIEKMLNDLSLIKRFE 297
           EE+ A++PSL  +GR  +DE ++++L+D+   + F+
Sbjct: 120 EESKALIPSL--EGR-FEDEELQQILDDIQTKRSFQ 141

BLAST of Lsi01G017460 vs. Swiss-Prot
Match: RPB4_DROME (DNA-directed RNA polymerase II 16 kDa polypeptide OS=Drosophila melanogaster GN=Rpb4 PE=2 SV=5)

HSP 1 Score: 52.8 bits (125), Expect = 7.7e-06
Identity = 32/89 (35.96%), Postives = 49/89 (55.06%), Query Frame = 1

Query: 201 VFEKSLHYVKRFSRYKNPDAVRQVREYPYIIIFFILWDGGRYQLAEFELCVLGNLCPETV 260
           VF K+  Y   F ++KN + +   R               + +L +FEL  LGNLCPE  
Sbjct: 57  VFMKTYAYTDSFRKFKNKETIMSARSLLM-----------QKKLHKFELAALGNLCPEAP 116

Query: 261 EEAIAMVPSLKTKGRAHDDEAIEKMLNDL 290
           EEA A++PSL  +GR  +DE + ++L+D+
Sbjct: 117 EEAKALIPSL--EGR-FEDEELRQILDDI 131

BLAST of Lsi01G017460 vs. TrEMBL
Match: A0A0A0L236_CUCSA (Uncharacterized protein OS=Cucumis sativus GN=Csa_4G419590 PE=4 SV=1)

HSP 1 Score: 301.2 bits (770), Expect = 1.4e-78
Identity = 145/201 (72.14%), Postives = 170/201 (84.58%), Query Frame = 1

Query: 1   MEYRISRYSTMVVEVDIDLPTSKKIPKHPSPIELIGLIFTLRRRCNHFLVSENGERQLVE 60
           MEY+ISRYST  VEVDID+P   KIPKHPSPIELIGL+FTLRRRCN F V ENGERQLVE
Sbjct: 1   MEYQISRYSTTAVEVDIDVPKWTKIPKHPSPIELIGLLFTLRRRCNRFQVLENGERQLVE 60

Query: 61  HKEIPKVPLCDHQFPAEMFNGVDNSLFHYLSHSFENYKPKIDAELVADALIERWIEIRDQ 120
            +EI KVPLCDHQFP E+FNG  +SLF YL  SFENY+ KIDAE+VA  LIE+WIEIR+Q
Sbjct: 61  EQEISKVPLCDHQFPVEIFNGDSSSLFQYLCCSFENYESKIDAEVVASVLIEQWIEIRNQ 120

Query: 121 VNSRSSDKVFSMTVNLTISNVVARHEIGSDRNVFGGEYFRIVPACGEVIRKTLKRLEMKE 180
           VNSRS DKVFSMTVNLTI++VV ++ +G D + FGGE  R+VPAC + ++K LKRLEM+E
Sbjct: 121 VNSRSRDKVFSMTVNLTITDVVLQYVVGEDGSTFGGEDHRVVPACDKALKKMLKRLEMRE 180

Query: 181 EDERICVICLDELKKKRNGRV 202
           EDER+CVICLDE+ KK+NGR+
Sbjct: 181 EDERVCVICLDEVGKKKNGRI 201

BLAST of Lsi01G017460 vs. TrEMBL
Match: A0A0A0K7V5_CUCSA (Uncharacterized protein OS=Cucumis sativus GN=Csa_6G014650 PE=4 SV=1)

HSP 1 Score: 152.9 bits (385), Expect = 6.1e-34
Identity = 80/97 (82.47%), Postives = 81/97 (83.51%), Query Frame = 1

Query: 200 RVFEKSLHYVKRFSRYKNPDAVRQVREYPYIIIFFILWDGGRYQLAEFELCVLGNLCPET 259
           +VFEKSL YVKRFSRYKNPDAVRQVRE              RYQLAEFELCVLGNLCPET
Sbjct: 53  QVFEKSLQYVKRFSRYKNPDAVRQVREVL-----------SRYQLAEFELCVLGNLCPET 112

Query: 260 VEEAIAMVPSLKTKGRAHDDEAIEKMLNDLSLIKRFE 297
           VEEAIAMVPSLKTKGR HDDEAIEKMLNDLSLIKRFE
Sbjct: 113 VEEAIAMVPSLKTKGRVHDDEAIEKMLNDLSLIKRFE 138

BLAST of Lsi01G017460 vs. TrEMBL
Match: B9H5K4_POPTR (Uncharacterized protein OS=Populus trichocarpa GN=POPTR_0005s08690g PE=4 SV=1)

HSP 1 Score: 152.9 bits (385), Expect = 6.1e-34
Identity = 81/97 (83.51%), Postives = 84/97 (86.60%), Query Frame = 1

Query: 200 RVFEKSLHYVKRFSRYKNPDAVRQVREYPYIIIFFILWDGGRYQLAEFELCVLGNLCPET 259
           +VFEKSL YVKRFSRYKNPDAVRQVRE        IL    RYQLAEFELCVLGNLCPET
Sbjct: 53  QVFEKSLQYVKRFSRYKNPDAVRQVRE--------IL---SRYQLAEFELCVLGNLCPET 112

Query: 260 VEEAIAMVPSLKTKGRAHDDEAIEKMLNDLSLIKRFE 297
           VEEAIAMVPS+KTKGRAHDDEAIEKMLNDLSLIK+FE
Sbjct: 113 VEEAIAMVPSIKTKGRAHDDEAIEKMLNDLSLIKKFE 138

BLAST of Lsi01G017460 vs. TrEMBL
Match: A5APH2_VITVI (Putative uncharacterized protein OS=Vitis vinifera GN=VIT_12s0028g01400 PE=4 SV=1)

HSP 1 Score: 152.9 bits (385), Expect = 6.1e-34
Identity = 81/97 (83.51%), Postives = 84/97 (86.60%), Query Frame = 1

Query: 200 RVFEKSLHYVKRFSRYKNPDAVRQVREYPYIIIFFILWDGGRYQLAEFELCVLGNLCPET 259
           +VFEKSL YVKRFSRYKNPDAVRQVRE        IL    RYQLAEFELCVLGNLCPET
Sbjct: 53  QVFEKSLQYVKRFSRYKNPDAVRQVRE--------IL---SRYQLAEFELCVLGNLCPET 112

Query: 260 VEEAIAMVPSLKTKGRAHDDEAIEKMLNDLSLIKRFE 297
           VEEAIAMVPS+KTKGRAHDDEAIEKMLNDLSLIK+FE
Sbjct: 113 VEEAIAMVPSIKTKGRAHDDEAIEKMLNDLSLIKKFE 138

BLAST of Lsi01G017460 vs. TrEMBL
Match: A0A067JW54_JATCU (Uncharacterized protein OS=Jatropha curcas GN=JCGZ_23577 PE=4 SV=1)

HSP 1 Score: 152.9 bits (385), Expect = 6.1e-34
Identity = 81/97 (83.51%), Postives = 84/97 (86.60%), Query Frame = 1

Query: 200 RVFEKSLHYVKRFSRYKNPDAVRQVREYPYIIIFFILWDGGRYQLAEFELCVLGNLCPET 259
           +VFEKSL YVKRFSRYKNPDAVRQVRE        IL    RYQLAEFELCVLGNLCPET
Sbjct: 79  QVFEKSLQYVKRFSRYKNPDAVRQVRE--------IL---SRYQLAEFELCVLGNLCPET 138

Query: 260 VEEAIAMVPSLKTKGRAHDDEAIEKMLNDLSLIKRFE 297
           VEEAIAMVPS+KTKGRAHDDEAIEKMLNDLSLIK+FE
Sbjct: 139 VEEAIAMVPSIKTKGRAHDDEAIEKMLNDLSLIKKFE 164

BLAST of Lsi01G017460 vs. TAIR10
Match: AT5G09920.1 (AT5G09920.1 RNA polymerase II, Rpb4, core protein)

HSP 1 Score: 150.6 bits (379), Expect = 1.5e-36
Identity = 79/97 (81.44%), Postives = 83/97 (85.57%), Query Frame = 1

Query: 200 RVFEKSLHYVKRFSRYKNPDAVRQVREYPYIIIFFILWDGGRYQLAEFELCVLGNLCPET 259
           +VFEKSL YVKRFSRYKNPDAVRQVRE        IL    R+QL EFELCVLGNLCPET
Sbjct: 53  QVFEKSLQYVKRFSRYKNPDAVRQVRE--------IL---SRHQLTEFELCVLGNLCPET 112

Query: 260 VEEAIAMVPSLKTKGRAHDDEAIEKMLNDLSLIKRFE 297
           VEEA+AMVPSLKTKGRAHDDEAIEKMLNDLSL+KRFE
Sbjct: 113 VEEAVAMVPSLKTKGRAHDDEAIEKMLNDLSLVKRFE 138

BLAST of Lsi01G017460 vs. TAIR10
Match: AT4G15950.1 (AT4G15950.1 RNA polymerase II, Rpb4, core protein)

HSP 1 Score: 52.8 bits (125), Expect = 4.3e-07
Identity = 31/95 (32.63%), Postives = 55/95 (57.89%), Query Frame = 1

Query: 202 FEKSLHYVKRFSRYKNPDAVRQVREYPYIIIFFILWDGGRYQLAEFELCVLGNLCPETVE 261
           ++++L YV+    Y NP +VR+V E P             Y +++ E+CV+ N   E+V+
Sbjct: 123 YDRALAYVESCVHYTNPQSVRKVLE-PL----------KTYGISDGEMCVIANASSESVD 182

Query: 262 EAIAMVPSLKTKGRAHDDEAIEKMLNDLSLIKRFE 297
           E +A +PSLKTK +   ++ ++  L +LS +K+ E
Sbjct: 183 EVLAFIPSLKTK-KEVINQPLQDALEELSKLKKSE 205

BLAST of Lsi01G017460 vs. NCBI nr
Match: gi|778697734|ref|XP_011654386.1| (PREDICTED: RING finger protein 141-like [Cucumis sativus])

HSP 1 Score: 301.2 bits (770), Expect = 2.0e-78
Identity = 145/201 (72.14%), Postives = 170/201 (84.58%), Query Frame = 1

Query: 1   MEYRISRYSTMVVEVDIDLPTSKKIPKHPSPIELIGLIFTLRRRCNHFLVSENGERQLVE 60
           MEY+ISRYST  VEVDID+P   KIPKHPSPIELIGL+FTLRRRCN F V ENGERQLVE
Sbjct: 1   MEYQISRYSTTAVEVDIDVPKWTKIPKHPSPIELIGLLFTLRRRCNRFQVLENGERQLVE 60

Query: 61  HKEIPKVPLCDHQFPAEMFNGVDNSLFHYLSHSFENYKPKIDAELVADALIERWIEIRDQ 120
            +EI KVPLCDHQFP E+FNG  +SLF YL  SFENY+ KIDAE+VA  LIE+WIEIR+Q
Sbjct: 61  EQEISKVPLCDHQFPVEIFNGDSSSLFQYLCCSFENYESKIDAEVVASVLIEQWIEIRNQ 120

Query: 121 VNSRSSDKVFSMTVNLTISNVVARHEIGSDRNVFGGEYFRIVPACGEVIRKTLKRLEMKE 180
           VNSRS DKVFSMTVNLTI++VV ++ +G D + FGGE  R+VPAC + ++K LKRLEM+E
Sbjct: 121 VNSRSRDKVFSMTVNLTITDVVLQYVVGEDGSTFGGEDHRVVPACDKALKKMLKRLEMRE 180

Query: 181 EDERICVICLDELKKKRNGRV 202
           EDER+CVICLDE+ KK+NGR+
Sbjct: 181 EDERVCVICLDEVGKKKNGRI 201

BLAST of Lsi01G017460 vs. NCBI nr
Match: gi|659111483|ref|XP_008455760.1| (PREDICTED: RING finger protein 141-like [Cucumis melo])

HSP 1 Score: 300.4 bits (768), Expect = 3.4e-78
Identity = 144/200 (72.00%), Postives = 171/200 (85.50%), Query Frame = 1

Query: 1   MEYRISRYSTMVVEVDIDLPTSKKIPKHPSPIELIGLIFTLRRRCNHFLVSENGERQLVE 60
           MEY+IS+YST  VE+DIDLP S++I KHPSPIELIGL+FTLRRRCN F V ENGERQLVE
Sbjct: 1   MEYQISQYSTTAVEIDIDLPMSRRIRKHPSPIELIGLLFTLRRRCNRFQVLENGERQLVE 60

Query: 61  HKEIPKVPLCDHQFPAEMFNGVDNSLFHYLSHSFENYKPKIDAELVADALIERWIEIRDQ 120
            +E  KVPLCDHQFP E+FNG DNSLF YL  SFENY+PKIDAE+VA ALIE+WI IRDQ
Sbjct: 61  EQEASKVPLCDHQFPVEIFNGDDNSLFQYLCCSFENYEPKIDAEVVATALIEQWIGIRDQ 120

Query: 121 VNSRSSDKVFSMTVNLTISNVVARHEIGSDRNVFGGEYFRIVPACGEVIRKTLKRLEMKE 180
           +NSRS  KVFSMTVNLTI++VV ++ IG D + FGGE FR VPAC + ++K LKRLE+++
Sbjct: 121 LNSRSCHKVFSMTVNLTITDVVVQYVIGEDGSSFGGEDFRAVPACDKALKKMLKRLEVRD 180

Query: 181 EDERICVICLDELKKKRNGR 201
           EDERIC+ICLDE++KK+NGR
Sbjct: 181 EDERICIICLDEVRKKKNGR 200

BLAST of Lsi01G017460 vs. NCBI nr
Match: gi|225446712|ref|XP_002282413.1| (PREDICTED: DNA-directed RNA polymerase II subunit 4 [Vitis vinifera])

HSP 1 Score: 152.9 bits (385), Expect = 8.7e-34
Identity = 81/97 (83.51%), Postives = 84/97 (86.60%), Query Frame = 1

Query: 200 RVFEKSLHYVKRFSRYKNPDAVRQVREYPYIIIFFILWDGGRYQLAEFELCVLGNLCPET 259
           +VFEKSL YVKRFSRYKNPDAVRQVRE        IL    RYQLAEFELCVLGNLCPET
Sbjct: 53  QVFEKSLQYVKRFSRYKNPDAVRQVRE--------IL---SRYQLAEFELCVLGNLCPET 112

Query: 260 VEEAIAMVPSLKTKGRAHDDEAIEKMLNDLSLIKRFE 297
           VEEAIAMVPS+KTKGRAHDDEAIEKMLNDLSLIK+FE
Sbjct: 113 VEEAIAMVPSIKTKGRAHDDEAIEKMLNDLSLIKKFE 138

BLAST of Lsi01G017460 vs. NCBI nr
Match: gi|643708828|gb|KDP23744.1| (hypothetical protein JCGZ_23577 [Jatropha curcas])

HSP 1 Score: 152.9 bits (385), Expect = 8.7e-34
Identity = 81/97 (83.51%), Postives = 84/97 (86.60%), Query Frame = 1

Query: 200 RVFEKSLHYVKRFSRYKNPDAVRQVREYPYIIIFFILWDGGRYQLAEFELCVLGNLCPET 259
           +VFEKSL YVKRFSRYKNPDAVRQVRE        IL    RYQLAEFELCVLGNLCPET
Sbjct: 79  QVFEKSLQYVKRFSRYKNPDAVRQVRE--------IL---SRYQLAEFELCVLGNLCPET 138

Query: 260 VEEAIAMVPSLKTKGRAHDDEAIEKMLNDLSLIKRFE 297
           VEEAIAMVPS+KTKGRAHDDEAIEKMLNDLSLIK+FE
Sbjct: 139 VEEAIAMVPSIKTKGRAHDDEAIEKMLNDLSLIKKFE 164

BLAST of Lsi01G017460 vs. NCBI nr
Match: gi|449441240|ref|XP_004138390.1| (PREDICTED: DNA-directed RNA polymerase II subunit 4 [Cucumis sativus])

HSP 1 Score: 152.9 bits (385), Expect = 8.7e-34
Identity = 80/97 (82.47%), Postives = 81/97 (83.51%), Query Frame = 1

Query: 200 RVFEKSLHYVKRFSRYKNPDAVRQVREYPYIIIFFILWDGGRYQLAEFELCVLGNLCPET 259
           +VFEKSL YVKRFSRYKNPDAVRQVRE              RYQLAEFELCVLGNLCPET
Sbjct: 53  QVFEKSLQYVKRFSRYKNPDAVRQVREVL-----------SRYQLAEFELCVLGNLCPET 112

Query: 260 VEEAIAMVPSLKTKGRAHDDEAIEKMLNDLSLIKRFE 297
           VEEAIAMVPSLKTKGR HDDEAIEKMLNDLSLIKRFE
Sbjct: 113 VEEAIAMVPSLKTKGRVHDDEAIEKMLNDLSLIKRFE 138

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
NRPB4_ARATH2.7e-3581.44DNA-directed RNA polymerase II subunit 4 OS=Arabidopsis thaliana GN=NRPB4 PE=1 S... [more]
RPB4_DICDI8.7e-1036.46DNA-directed RNA polymerase II subunit rpb4 OS=Dictyostelium discoideum GN=polr2... [more]
RPB4_HUMAN2.5e-0938.54DNA-directed RNA polymerase II subunit RPB4 OS=Homo sapiens GN=POLR2D PE=1 SV=1[more]
RPB4_MOUSE2.5e-0938.54DNA-directed RNA polymerase II subunit RPB4 OS=Mus musculus GN=Polr2d PE=1 SV=2[more]
RPB4_DROME7.7e-0635.96DNA-directed RNA polymerase II 16 kDa polypeptide OS=Drosophila melanogaster GN=... [more]
Match NameE-valueIdentityDescription
A0A0A0L236_CUCSA1.4e-7872.14Uncharacterized protein OS=Cucumis sativus GN=Csa_4G419590 PE=4 SV=1[more]
A0A0A0K7V5_CUCSA6.1e-3482.47Uncharacterized protein OS=Cucumis sativus GN=Csa_6G014650 PE=4 SV=1[more]
B9H5K4_POPTR6.1e-3483.51Uncharacterized protein OS=Populus trichocarpa GN=POPTR_0005s08690g PE=4 SV=1[more]
A5APH2_VITVI6.1e-3483.51Putative uncharacterized protein OS=Vitis vinifera GN=VIT_12s0028g01400 PE=4 SV=... [more]
A0A067JW54_JATCU6.1e-3483.51Uncharacterized protein OS=Jatropha curcas GN=JCGZ_23577 PE=4 SV=1[more]
Match NameE-valueIdentityDescription
AT5G09920.11.5e-3681.44 RNA polymerase II, Rpb4, core protein[more]
AT4G15950.14.3e-0732.63 RNA polymerase II, Rpb4, core protein[more]
Match NameE-valueIdentityDescription
gi|778697734|ref|XP_011654386.1|2.0e-7872.14PREDICTED: RING finger protein 141-like [Cucumis sativus][more]
gi|659111483|ref|XP_008455760.1|3.4e-7872.00PREDICTED: RING finger protein 141-like [Cucumis melo][more]
gi|225446712|ref|XP_002282413.1|8.7e-3483.51PREDICTED: DNA-directed RNA polymerase II subunit 4 [Vitis vinifera][more]
gi|643708828|gb|KDP23744.1|8.7e-3483.51hypothetical protein JCGZ_23577 [Jatropha curcas][more]
gi|449441240|ref|XP_004138390.1|8.7e-3482.47PREDICTED: DNA-directed RNA polymerase II subunit 4 [Cucumis sativus][more]
The following terms have been associated with this gene:
Vocabulary: Biological Process
TermDefinition
GO:0044237cellular metabolic process
GO:0006351transcription, DNA-templated
Vocabulary: Molecular Function
TermDefinition
GO:0003824catalytic activity
GO:0000166nucleotide binding
GO:0003899DNA-directed RNA polymerase activity
Vocabulary: INTERPRO
TermDefinition
IPR010997HRDC-like_sf
IPR006590RNA_pol_Rpb4/RPC9_core
IPR005574RPB4/RPC9
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0031990 mRNA export from nucleus in response to heat stress
biological_process GO:0008150 biological_process
biological_process GO:0045948 positive regulation of translational initiation
biological_process GO:0006144 purine nucleobase metabolic process
biological_process GO:0006206 pyrimidine nucleobase metabolic process
biological_process GO:0034402 recruitment of 3'-end processing factors to RNA polymerase II holoenzyme complex
biological_process GO:0006367 transcription initiation from RNA polymerase II promoter
biological_process GO:0006351 transcription, DNA-templated
biological_process GO:0000288 nuclear-transcribed mRNA catabolic process, deadenylation-dependent decay
biological_process GO:0044237 cellular metabolic process
cellular_component GO:0000932 cytoplasmic mRNA processing body
cellular_component GO:0005665 DNA-directed RNA polymerase II, core complex
cellular_component GO:0005730 nucleolus
cellular_component GO:0005575 cellular_component
molecular_function GO:0005515 protein binding
molecular_function GO:0000166 nucleotide binding
molecular_function GO:0008270 zinc ion binding
molecular_function GO:0031369 translation initiation factor binding
molecular_function GO:0003727 single-stranded RNA binding
molecular_function GO:0003697 single-stranded DNA binding
molecular_function GO:0003899 DNA-directed RNA polymerase activity
molecular_function GO:0003824 catalytic activity

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Lsi01G017460.1Lsi01G017460.1mRNA


Analysis Name: InterPro Annotations of Lagenaria siceraria
Date Performed: 2017-09-18
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR005574RNA polymerase II, Rpb4PFAMPF03874RNA_pol_Rpb4coord: 168..290
score: 1.1
IPR006590RNA polymerase II, Rpb4, coreSMARTSM00657rpol4neu2coord: 178..296
score: 1.8
IPR010997HRDC-likeunknownSSF47819HRDC-likecoord: 190..293
score: 6.1
NoneNo IPR availablePANTHERPTHR21297DNA-DIRECTED RNA POLYMERASE IIcoord: 201..296
score: 6.1

The following gene(s) are paralogous to this gene:

None