Lsi01G011640 (gene) Bottle gourd (USVL1VR-Ls)

Overview
NameLsi01G011640
Typegene
OrganismLagenaria siceraria (Bottle gourd (USVL1VR-Ls))
DescriptionCysteine proteinase inhibitor 5
Locationchr01 : 9796653 .. 9798139 (-)
Genome Viewer
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: five_prime_UTRCDS
Hold the cursor over a type above to highlight its positions in the sequence below.
GTTTTGTAAGGAAGCACACAAAACAAAAGCATTCCATAAGTAATAAGTTTGTGGAAATAAGTTTGTATATTGAAAGTAAGTTAAGAAAATGTGTTCTCAAGTGCCCGGCGGTTATGGTCCATGTGAGGATCCCGAAGGCCCACACATAAAAGAAATAGCAGAATGGGCAGTAGCAGAGTACAACAAAGGCGGACATTGCTTGACTTTGATTTATGTGTTAAGTTGTGAGTCACAGGTGGTGGCCGGAGTCAACTACCGCCTTGTCTTGTTGGTTAAGGATGAAAATAATCATGAGAAAGTCTACGAGACTGTTGTGTGGGAGAAGATATGGGAGAATTTTAGGGAGCTCATTTACTTTAAACCTTACGTGGTATGCTTTTTCTTATAGCACTTATATGCTTTCTTTTGCAACCCTTTTCAAATTTGTTATTTGTAATATCTATATCTTGTTCCTTAATGAAGTGTCCTCTAACTTATCAAACAAAATAAAATTCATTAATTGAGTTTGGTAAAGTTGAGTCTATTTTAATCAAATTAAAAATAGGATGATATATATTAGTTTGTACTAGGAATTCCAAAATTATTTTATCTTAATATAAAGCTATTTTTCAGTCTTAATGATCATTTAAGAAAGTACACTAAAAGTGGAATAAAAATTGGAGTAGTATAAATTTGAAACTCTCTTGTTTTGAAAAGAAGCACAAAACATTTCATAGGGTGCATTTGGTTTAAATTTTCAAGTGTTTAATTTTGAAATAAGTCATTTTAGAAAAAACTAAAGCGTTTGATAACCGTTCAAAATAGCTTTTGAAATACTCTCAAAGTATATTTCAAACATTTTTTTTTATATATAAAAGAGTTTAAATAATAATGATTTTTTTTAAAAAAATATATCTTTTCTAAACAATCAAAATATCTAAGTGGCTGTTTGGGGGACAGTAATTAGATGGGTTTGGGTTGTAATGTAATCTAAGATCCACGTTTGGATTGAATATTTTAGGCTCGATTTGTAATACTAAACTCATTCCGTTCTCACAGTTTCCAATCAAACTCTCTTTCCCACCGTTTTCATGCTTCATGATCCCATTTTCGTTATGTCATCAATTCCTCACACATTCGAACAACTCTCAGATTCCCAATAATCTATCAGCCCCAAACAATCCCTAATTATGTATATTGAAATTAAGTTAGGAAAAATGTGTTCTCAGGTAGTTGGCGGTTATGGTCCATGTAAGGATCCTGAAAGCCCACATGTGAAAGAAATAGCAGAATGGGCAGTAGCAGAGTACAACAAAGGCGGATATTGCTTGACATTGATTAGTGTGTTGAAATGCGAGTCACAGGTGGTGGCCGGAATCAACTACCGCCTTGTCTTGTTGGCTAAAAATGACAAAAATCATGAGGGAAAATATGAGACTGTTGTGTGGGAGAAGATATGGGAGCATTTCAGGAAGCTCATTAGCTTTAAACCTCTCTTGTTATGTTGA

mRNA sequence

GTTTTGTAAGGAAGCACACAAAACAAAAGCATTCCATAAGTAATAAGTTTGTGGAAATAAGTTTGTATATTGAAAGTAAGTTAAGAAAATGTGTTCTCAAGTGCCCGGCGGTTATGGTCCATGTGAGGATCCCGAAGGCCCACACATAAAAGAAATAGCAGAATGGGCAGTAGCAGAGTACAACAAAGGCGGACATTGCTTGACTTTGATTTATGTGTTAAGTTGTGAGTCACAGGTGGTGGCCGGAGTCAACTACCGCCTTGTCTTGTTGGTTAAGGATGAAAATAATCATGAGAAAGTCTACGAGACTGTTGTGTGGGAGAAGATATGGGAGAATTTTAGGGAGCTCATTTACTTTAAACCTTACGTGGATCCTGAAAGCCCACATGTGAAAGAAATAGCAGAATGGGCAGTAGCAGAGTACAACAAAGGCGGATATTGCTTGACATTGATTAGTGTGTTGAAATGCGAGTCACAGGTGGTGGCCGGAATCAACTACCGCCTTGTCTTGTTGGCTAAAAATGACAAAAATCATGAGGGAAAATATGAGACTGTTGTGTGGGAGAAGATATGGGAGCATTTCAGGAAGCTCATTAGCTTTAAACCTCTCTTGTTATGTTGA

Coding sequence (CDS)

ATGTGTTCTCAAGTGCCCGGCGGTTATGGTCCATGTGAGGATCCCGAAGGCCCACACATAAAAGAAATAGCAGAATGGGCAGTAGCAGAGTACAACAAAGGCGGACATTGCTTGACTTTGATTTATGTGTTAAGTTGTGAGTCACAGGTGGTGGCCGGAGTCAACTACCGCCTTGTCTTGTTGGTTAAGGATGAAAATAATCATGAGAAAGTCTACGAGACTGTTGTGTGGGAGAAGATATGGGAGAATTTTAGGGAGCTCATTTACTTTAAACCTTACGTGGATCCTGAAAGCCCACATGTGAAAGAAATAGCAGAATGGGCAGTAGCAGAGTACAACAAAGGCGGATATTGCTTGACATTGATTAGTGTGTTGAAATGCGAGTCACAGGTGGTGGCCGGAATCAACTACCGCCTTGTCTTGTTGGCTAAAAATGACAAAAATCATGAGGGAAAATATGAGACTGTTGTGTGGGAGAAGATATGGGAGCATTTCAGGAAGCTCATTAGCTTTAAACCTCTCTTGTTATGTTGA

Protein sequence

MCSQVPGGYGPCEDPEGPHIKEIAEWAVAEYNKGGHCLTLIYVLSCESQVVAGVNYRLVLLVKDENNHEKVYETVVWEKIWENFRELIYFKPYVDPESPHVKEIAEWAVAEYNKGGYCLTLISVLKCESQVVAGINYRLVLLAKNDKNHEGKYETVVWEKIWEHFRKLISFKPLLLC
Homology
[Close]
BLAST of Lsi01G011640 vs. Swiss-Prot
Match: CYTM_SOLTU (Multicystatin OS=Solanum tuberosum PE=1 SV=1)

HSP 1 Score: 105.1 bits (261), Expect = 7.8e-22
Identity = 60/185 (32.43%), Postives = 98/185 (52.97%), Query Frame = 1 

Query: 3   SQVPGGYGPCEDPEGPHIKEIAEWAVAEYNKGGHC-LTLIYVLSCESQVVAGVNYRLVLL 62
           S +PGG     +P     +E+A +A+ +YNK  +  L  +  L+ + QVVAG+ Y + L 
Sbjct: 95  STMPGGIVNVPNPNNTKFQELARFAIQDYNKKQNAHLEFVENLNVKEQVVAGIMYYITLA 154

Query: 63  VKDENNHEKVYETVVWEKIWENFRELIYFKPYVD-------------PESPHVKEIAEWA 122
             D+   +K+Y+  +W K WE+F++++ FK   D             P +P  +++A +A
Sbjct: 155 ATDDAGKKKIYKAKIWVKEWEDFKKVVEFKLVGDDIAKLGGITDVPFPNNPEFQDLARFA 214

Query: 123 VAEYN-KGGYCLTLISVLKCESQVVAGINYRLVLLAKNDKNHEGKYETVVWEKIWEHFRK 173
           +  YN K    L  +  L  + QVVAG+ Y  + LA  D   +  YET +W K WE F+K
Sbjct: 215 IQVYNKKENVHLEFVENLNVKQQVVAGMMY-YITLAAIDAGKKKIYETKIWVKEWEDFKK 274

[Close]
BLAST of Lsi01G011640 vs. Swiss-Prot
Match: CYT5_ARATH (Cysteine proteinase inhibitor 5 OS=Arabidopsis thaliana GN=CYS5 PE=2 SV=2)

HSP 1 Score: 70.1 bits (170), Expect = 2.8e-11
Identity = 37/87 (42.53%), Postives = 50/87 (57.47%), Query Frame = 1 

Query: 7   GGYGPCEDPEGPHIKEIAEWAVAEYNKGGHC-LTLIYVLSCESQVVAGVNYRLVLLVKDE 66
           GG+ P  +   P + EI E+AV+EYNK     L    V+S E+QVV+G NYRL +   D 
Sbjct: 29  GGWSPISNVTDPQVVEIGEFAVSEYNKRSESGLKFETVVSGETQVVSGTNYRLKVAANDG 88

Query: 67  NNHEKVYETVVWEKIWENFRELIYFKP 93
           +   K Y  +VW+K W  FR L  F+P
Sbjct: 89  DGVSKNYLAIVWDKPWMKFRNLTSFEP 115

[Close]
BLAST of Lsi01G011640 vs. Swiss-Prot
Match: CYT7_ORYSJ (Putative cysteine proteinase inhibitor 7 OS=Oryza sativa subsp. japonica GN=Os03g0210100 PE=3 SV=1)

HSP 1 Score: 69.3 bits (168), Expect = 4.7e-11
Identity = 39/84 (46.43%), Postives = 53/84 (63.10%), Query Frame = 1 

Query: 94  VDPESPHVKEIAEWAVAEYNKGGYC--LTLISVLKCESQVVAGINYRLVLLAKNDKNHEG 153
           VD   PHV+E+  WAVAE ++G     LT   V   E QVVAG+NYRL L A +    +G
Sbjct: 33  VDINDPHVQELGRWAVAEEDRGVAAGGLTFERVTDGEKQVVAGVNYRLTLEASSSGAKDG 92

Query: 154 KYETVVWEK-IWEHFRKLISFKPL 175
           +YE VV+E+    + RKL+SF+P+
Sbjct: 93  RYEAVVYEQDPRSNARKLVSFEPI 116

[Close]
BLAST of Lsi01G011640 vs. Swiss-Prot
Match: CYT1_ACTDE (Cysteine proteinase inhibitor 1 OS=Actinidia deliciosa PE=1 SV=1)

HSP 1 Score: 68.9 bits (167), Expect = 6.2e-11
Identity = 39/84 (46.43%), Postives = 54/84 (64.29%), Query Frame = 1 

Query: 90  FKPYVDPESPHVKEIAEWAVAEYNKGGYC-LTLISVLKCESQVVAGINYRLVLLAKNDKN 149
           ++P     S  V+++A++AV+E+NK     L   SV++  +QVVAG NYRLV+ AK D  
Sbjct: 32  WRPIESLNSAEVQDVAQFAVSEHNKQANDELQYQSVVRGYTQVVAGTNYRLVIAAK-DGA 91

Query: 150 HEGKYETVVWEKIWEHFRKLISFK 173
             G YE VVW+K W HFR L SF+
Sbjct: 92  VVGNYEAVVWDKPWMHFRNLTSFR 114

[Close]
BLAST of Lsi01G011640 vs. Swiss-Prot
Match: CYT8_ORYSJ (Cysteine proteinase inhibitor 8 OS=Oryza sativa subsp. japonica GN=Os03g0429000 PE=2 SV=1)

HSP 1 Score: 64.3 bits (155), Expect = 1.5e-09
Identity = 36/87 (41.38%), Postives = 47/87 (54.02%), Query Frame = 1 

Query: 7   GGYGPCEDPEGPHIKEIAEWAVAEY-NKGGHCLTLIYVLSCESQVVAGVNYRLVLLVKDE 66
           GG+ P  D   PHI+E+  WAV  + +     L    V S E QVV+G+NYRLV+   D 
Sbjct: 33  GGWSPITDVGDPHIQELGGWAVERHASLSSDGLRFRRVTSGEQQVVSGMNYRLVVSASDP 92

Query: 67  NNHEKVYETVVWEKIWENFRELIYFKP 93
                 Y  VV+E+ W N R+L  FKP
Sbjct: 93  AGATASYVAVVYEQSWTNTRQLTSFKP 119

[Close]
BLAST of Lsi01G011640 vs. TrEMBL
Match: V4T622_9ROSI (Cysteine proteinase inhibitor OS=Citrus clementina GN=CICLE_v10002632mg PE=3 SV=1)

HSP 1 Score: 148.7 bits (374), Expect = 6.8e-33
Identity = 86/180 (47.78%), Postives = 114/180 (63.33%), Query Frame = 1 

Query: 7   GGYGPCEDPEGPHIKEIAEWAVAEYNKGGH-CLTLIYVLSCESQVVAGVNYRLVLLVKDE 66
           GG+ P EDP+  H+ EI ++AV EYNK     L    V   E+QVV+G NYRL+L+VKD 
Sbjct: 3   GGWKPIEDPKEKHVMEIGQFAVTEYNKRSKSALKFESVEKGETQVVSGTNYRLILVVKDG 62

Query: 67  NNHEKVYETVVWEKIWENFRELIYFKPYV---------DPESPHVKEIAEWAVAEYNKGG 126
            + +K +E VV EK WE+F+ L  FKP           DP+  HV EI ++AV EYNK  
Sbjct: 63  PSTKK-FEAVVLEKPWEHFKSLTSFKPMPMLGGWKPIEDPKEKHVMEIGQFAVTEYNKRS 122

Query: 127 Y-CLTLISVLKCESQVVAGINYRLVLLAKNDKNHEGKYETVVWEKIWEHFRKLISFKPLL 176
              L   SV K E+QVV+G NYRL+L+ K+  + + K+E VVWEK WEHF+ L SFKP++
Sbjct: 123 KGALKFESVEKGETQVVSGTNYRLILVVKDGPSTK-KFEAVVWEKPWEHFKSLTSFKPMV 180

[Close]
BLAST of Lsi01G011640 vs. TrEMBL
Match: O80389_CUCSA (Cysteine proteinase inhibitor OS=Cucumis sativus PE=2 SV=1)

HSP 1 Score: 136.3 bits (342), Expect = 3.5e-29
Identity = 66/91 (72.53%), Postives = 73/91 (80.22%), Query Frame = 1 

Query: 1  MCSQVPGGYGPCEDPEGPHIKEIAEWAVAEYNKG-GHCLTLIYVLSCESQVVAGVNYRLV 60
          M S   GGY PC+DP  PH+K+IAEWAVAEYNK  GH LTL+ +L CESQVVAGVN+RLV
Sbjct: 1  MSSSEIGGYVPCKDPNDPHVKDIAEWAVAEYNKSQGHHLTLVSILKCESQVVAGVNWRLV 60

Query: 61 LLVKDENNHEKVYETVVWEKIWENFRELIYF 91
          L  KDENN E  YETVVWEKIWENFR+LI F
Sbjct: 61 LKCKDENNGEGNYETVVWEKIWENFRQLITF 91

[Close]
BLAST of Lsi01G011640 vs. TrEMBL
Match: A0A078EQE2_BRANA (Cysteine proteinase inhibitor OS=Brassica napus GN=BnaA09g19480D PE=3 SV=1)

HSP 1 Score: 123.6 bits (309), Expect = 2.4e-25
Identity = 77/187 (41.18%), Postives = 105/187 (56.15%), Query Frame = 1 

Query: 7   GGYGPCEDPEGPHIKEIAEWAVAEYNKGGHC-LTLIYVLSCESQVVAGVNYRLVLLVKD- 66
           GG+ P  D +  HI EI E+AV+EYNK     L  + V+S ESQ+VAG+NYRL+L   + 
Sbjct: 27  GGWSPISDVKNAHIVEIGEFAVSEYNKQSKSGLKFVEVVSGESQIVAGMNYRLILAANEG 86

Query: 67  ----ENNHEKVYETVVWEKIWENFRELIYFKP------YVDPESPHVKEIAEWAVAEYNK 126
                N   K YE VVWEK W     L  FKP       +  ++   ++I E+AV+EY+K
Sbjct: 87  VAIAGNGESKKYEAVVWEKPWLKSMNLTSFKPAMLTRKQLRHKALTTQKIGEFAVSEYDK 146

Query: 127 GGYC-LTLISVLKCESQVVAGINYRLVLLAKND----KNHEG-KYETVVWEKIWEHFRKL 176
                L  + V+  ESQ+VAG+NYRL++ A       +N E  KYE +VW+K W     L
Sbjct: 147 QSKSGLKFVEVVSGESQIVAGMNYRLIVAANEGIPIARNGESKKYEAIVWDKPWLKSMNL 206

[Close]
BLAST of Lsi01G011640 vs. TrEMBL
Match: A0A0A0KIY5_CUCSA (Cysteine proteinase inhibitor OS=Cucumis sativus GN=Csa_6G483248 PE=3 SV=1)

HSP 1 Score: 109.8 bits (273), Expect = 3.5e-21
Identity = 52/93 (55.91%), Postives = 67/93 (72.04%), Query Frame = 1 

Query: 7   GGYGPCEDPEGPHIKEIAEWAVAEYNKG-GHCLTLIYVLSCESQVVAGVNYRLVLLVKDE 66
           GGY P +DP+   +KE+AEWAV EYNK  G  L  + +L CESQ+V GVNYR +L VKDE
Sbjct: 10  GGYTPVKDPQSLRMKELAEWAVEEYNKKEGTHLRFVCILVCESQIVEGVNYRFILRVKDE 69

Query: 67  NNHEKVYETVVWEKIWENFRELIYFKPYVDPES 99
           N++E  YE +VWEK WE+F+ L+YFK  +  ES
Sbjct: 70  NDNEGNYEAIVWEKTWEHFKGLVYFKQLLLTES 102

[Close]
BLAST of Lsi01G011640 vs. TrEMBL
Match: M0U917_MUSAM (Cysteine proteinase inhibitor OS=Musa acuminata subsp. malaccensis PE=3 SV=1)

HSP 1 Score: 107.8 bits (268), Expect = 1.3e-20
Identity = 64/127 (50.39%), Postives = 86/127 (67.72%), Query Frame = 1 

Query: 54  VNYRLVLLVKDENNHEK----VYETVVWEKIWENF---RELIYFKPYVDPESPHVKEIAE 113
           +NY L L VKD  +  K    VYE++  EK+ E+F   +E       VD  +PHV +IA 
Sbjct: 1   MNYNLTLKVKDGLSTAKYVAVVYESLKGEKVLESFVLIQEKFDGWTPVDVNNPHVHDIAV 60

Query: 114 WAVAEYNKGGY-CLTLISVLKCESQVVAGINYRLVLLAKNDKNHEGKYETVVWEKIWEHF 173
           +AV+E+NK     LTL++V+K +SQVVAG+NY+L+L+AKN+K     YE VVWEK WE+F
Sbjct: 61  FAVSEHNKEAKEPLTLVNVVKGQSQVVAGVNYKLLLVAKNEKGASAGYEAVVWEKEWENF 120

[Close]
BLAST of Lsi01G011640 vs. TAIR10
Match: AT5G47550.1 (AT5G47550.1 Cystatin/monellin superfamily protein)

HSP 1 Score: 70.1 bits (170), Expect = 1.6e-12
Identity = 37/87 (42.53%), Postives = 50/87 (57.47%), Query Frame = 1 

Query: 7   GGYGPCEDPEGPHIKEIAEWAVAEYNKGGHC-LTLIYVLSCESQVVAGVNYRLVLLVKDE 66
           GG+ P  +   P + EI E+AV+EYNK     L    V+S E+QVV+G NYRL +   D 
Sbjct: 29  GGWSPISNVTDPQVVEIGEFAVSEYNKRSESGLKFETVVSGETQVVSGTNYRLKVAANDG 88

Query: 67  NNHEKVYETVVWEKIWENFRELIYFKP 93
           +   K Y  +VW+K W  FR L  F+P
Sbjct: 89  DGVSKNYLAIVWDKPWMKFRNLTSFEP 115

[Close]
BLAST of Lsi01G011640 vs. TAIR10
Match: AT3G12490.2 (AT3G12490.2 cystatin B)

HSP 1 Score: 57.0 bits (136), Expect = 1.4e-08
Identity = 55/187 (29.41%), Postives = 78/187 (41.71%), Query Frame = 1 

Query: 7   GGYG--PCEDPEGPHIKEIAEWAVAEYNKGGHCLT-LIYVLSCESQVVAGVNYRLVLLVK 66
           GG G  P     G  ++ +A +AV E+NK  + L     V+  + QVVAG  + L L + 
Sbjct: 38  GGVGDVPANQNSG-EVESLARFAVDEHNKKENALLEFARVVKAKEQVVAGTLHHLTLEIL 97

Query: 67  DENNHEKVYETVVWEKIWENFRELIYFKPYVDP------------------------ESP 126
            E   +K+YE  VW K W NF+EL  FKP  D                         + P
Sbjct: 98  -EAGQKKLYEAKVWVKPWLNFKELQEFKPASDAPAITSSDLGCKQGEHESGWREVPGDDP 157

Query: 127 HVKEIAEWAVAEYNKGGYCL---TLISVLKCESQVVA-GINYRLVLLAKN---------- 152
            VK +AE AV    +    L    L+ V+  +++V      Y ++L  K           
Sbjct: 158 EVKHVAEQAVKTIQQRSNSLFPYELLEVVHAKAEVTGEAAKYNMLLKLKRGEKEEKFKVE 217

[Close]
BLAST of Lsi01G011640 vs. TAIR10
Match: AT4G16500.1 (AT4G16500.1 Cystatin/monellin superfamily protein)

HSP 1 Score: 56.6 bits (135), Expect = 1.8e-08
Identity = 32/85 (37.65%), Postives = 47/85 (55.29%), Query Frame = 1 

Query: 91  KPYVDPESPHVKEIAEWAVAEYNKGGY-CLTLISVLKCESQVVAGINYRLVLLAKNDKNH 150
           KP  +   P V  +A++A+ E+NK     L  + V++  +QVV+G  Y L + AK+    
Sbjct: 33  KPIKNVSDPDVVAVAKYAIEEHNKESKEKLVFVKVVEGTTQVVSGTKYDLKIAAKDGGGK 92

Query: 151 EGKYETVVWEKIWEHFRKLISFKPL 175
              YE VV EK+W H + L SFK L
Sbjct: 93  IKNYEAVVVEKLWLHSKSLESFKAL 117

[Close]
BLAST of Lsi01G011640 vs. TAIR10
Match: AT5G05110.1 (AT5G05110.1 Cystatin/monellin family protein)

HSP 1 Score: 54.3 bits (129), Expect = 8.9e-08
Identity = 35/76 (46.05%), Postives = 44/76 (57.89%), Query Frame = 1 

Query: 17  GPHIKEIAEWAVAEYNKGGHC-LTLIYVLSCESQVVAGVNYRLVLLVKDENNHEKVYETV 76
           G  I +IA +AV E+N+  +  L L  VL    QVVAG  YRL L V  E   +K+YE  
Sbjct: 58  GKEIDDIALFAVQEHNRRENAVLELARVLKATEQVVAGKLYRLTLEVI-EAGEKKIYEAK 117

Query: 77  VWEKIWENFRELIYFK 92
           VW K W NF++L  FK
Sbjct: 118 VWVKPWMNFKQLQEFK 132

[Close]
BLAST of Lsi01G011640 vs. TAIR10
Match: AT5G12140.1 (AT5G12140.1 cystatin-1)

HSP 1 Score: 48.9 bits (115), Expect = 3.7e-06
Identity = 31/73 (42.47%), Postives = 45/73 (61.64%), Query Frame = 1 

Query: 20 IKEIAEWAVAEYNKGGHCLTLIY--VLSCESQVVAGVNYRLVLLVKDENNHEKVYETVVW 79
          ++ +A +AV E+NK  + LTL Y  +L  ++QVVAG  + L + V D   + KVYE  V 
Sbjct: 25 VESLARFAVDEHNKNEN-LTLEYKRLLGAKTQVVAGTMHHLTVEVADGETN-KVYEAKVL 84

Query: 80 EKIWENFRELIYF 91
          EK WEN ++L  F
Sbjct: 85 EKAWENLKQLESF 95

[Close]
BLAST of Lsi01G011640 vs. NCBI nr
Match: gi|695018835|ref|XP_009396392.1| (PREDICTED: cysteine proteinase inhibitor 1 {ECO:0000303|PubMed:14697268}-like [Musa acuminata subsp. malaccensis])

HSP 1 Score: 152.1 bits (383), Expect = 8.9e-34
Identity = 93/180 (51.67%), Postives = 116/180 (64.44%), Query Frame = 1 

Query: 7   GGYGPCEDPEGPHIKEIAEWAVAEYNKGGHC-LTLIYVLSCESQVVAGVNYRLVLLVKDE 66
           GG+   ED + PH++EIAE+AV+E NK     LTL  V   E+QVVAG NYRLVL V+D 
Sbjct: 33  GGWTAIEDLKDPHVREIAEFAVSENNKLEKTNLTLRKVEGGETQVVAGTNYRLVLEVRDG 92

Query: 67  NNHEKVYETVVWEKIWENFRELIYF---------KPYVDPESPHVKEIAEWAVAEYN-KG 126
           +     YE VVWEK WE+F++L  F          P  +   P V EIAE+AVAE+N + 
Sbjct: 93  SGASARYEAVVWEKPWESFKQLASFHKLVIVGGWTPIKNISDPDVGEIAEFAVAEHNHEA 152

Query: 127 GYCLTLISVLKCESQVVAGINYRLVLLAKN-DKNHEGKYETVVWEKIWEHFRKLISFKPL 175
           G  LTL  V+K E+QVVAGINY+LV+ AK+       +YE VVWEK WEHFR+L SFK L
Sbjct: 153 GTNLTLCKVVKGETQVVAGINYKLVIEAKDGGVGVVSEYEAVVWEKTWEHFRRLTSFKLL 212

[Close]
BLAST of Lsi01G011640 vs. NCBI nr
Match: gi|568825922|ref|XP_006467325.1| (PREDICTED: multicystatin [Citrus sinensis])

HSP 1 Score: 150.2 bits (378), Expect = 3.4e-33
Identity = 85/176 (48.30%), Postives = 112/176 (63.64%), Query Frame = 1 

Query: 7   GGYGPCEDPEGPHIKEIAEWAVAEYNKGGH-CLTLIYVLSCESQVVAGVNYRLVLLVKDE 66
           GG+ P EDP+  H+ EI ++AV EYNK     L    V   E+QVV+G NYRL+L+VKD 
Sbjct: 30  GGWKPIEDPKEKHVMEIGQFAVTEYNKQSKSALKFESVEKGETQVVSGTNYRLILVVKDG 89

Query: 67  NNHEKVYETVVWEKIWENFRELIYFKPY------VDPESPHVKEIAEWAVAEYNKGGY-C 126
            + +K +E VVWEK WE+F+ L  FKP       +DP   HV EI ++AV EYNK     
Sbjct: 90  PSTKK-FEAVVWEKPWEHFKSLTSFKPMLGGWTPIDPTEKHVMEIGQFAVTEYNKRSKSA 149

Query: 127 LTLISVLKCESQVVAGINYRLVLLAKNDKNHEGKYETVVWEKIWEHFRKLISFKPL 175
           L   SV K  +QVV+G NYRL+L+ K++ + + K   VVWEK WEHF+ L SFKP+
Sbjct: 150 LKFESVEKAWTQVVSGTNYRLILVVKDEPSTK-KLLVVVWEKPWEHFKSLTSFKPM 203

[Close]
BLAST of Lsi01G011640 vs. NCBI nr
Match: gi|568837934|ref|XP_006472972.1| (PREDICTED: multicystatin-like, partial [Citrus sinensis])

HSP 1 Score: 150.2 bits (378), Expect = 3.4e-33
Identity = 85/178 (47.75%), Postives = 113/178 (63.48%), Query Frame = 1 

Query: 5   VPGGYGPCEDPEGPHIKEIAEWAVAEYNKGGHC-LTLIYVLSCESQVVAGVNYRLVLLVK 64
           +PGG+ P  DP   H+ EI ++AV EYNK   C      V +  +QVV+G NYRL+L+VK
Sbjct: 1   MPGGWTPI-DPTEKHVMEIGQFAVTEYNKRSKCAFKFESVENAWTQVVSGTNYRLILIVK 60

Query: 65  DENNHEKVYETVVWEKIWENFRELIYFKPY------VDPESPHVKEIAEWAVAEYNKGGY 124
           D  + +K +E VVWEK WE+F+ L  FKP       +DP   HV  I ++AV EYNK   
Sbjct: 61  DGASTKK-FEAVVWEKPWEHFKSLTSFKPMPGGWTPIDPTEKHVMVIVQFAVTEYNKRSK 120

Query: 125 -CLTLISVLKCESQVVAGINYRLVLLAKNDKNHEGKYETVVWEKIWEHFRKLISFKPL 175
             L   SV K E+QVV+G NYRL+L+ K+  + + K+E VVWEK WEHF+ L SFKP+
Sbjct: 121 SALKFESVEKAETQVVSGTNYRLILVVKDGLSTK-KFEAVVWEKPWEHFKSLTSFKPM 175

[Close]
BLAST of Lsi01G011640 vs. NCBI nr
Match: gi|567878425|ref|XP_006431771.1| (hypothetical protein CICLE_v10002632mg [Citrus clementina])

HSP 1 Score: 148.7 bits (374), Expect = 9.8e-33
Identity = 86/180 (47.78%), Postives = 114/180 (63.33%), Query Frame = 1 

Query: 7   GGYGPCEDPEGPHIKEIAEWAVAEYNKGGH-CLTLIYVLSCESQVVAGVNYRLVLLVKDE 66
           GG+ P EDP+  H+ EI ++AV EYNK     L    V   E+QVV+G NYRL+L+VKD 
Sbjct: 3   GGWKPIEDPKEKHVMEIGQFAVTEYNKRSKSALKFESVEKGETQVVSGTNYRLILVVKDG 62

Query: 67  NNHEKVYETVVWEKIWENFRELIYFKPYV---------DPESPHVKEIAEWAVAEYNKGG 126
            + +K +E VV EK WE+F+ L  FKP           DP+  HV EI ++AV EYNK  
Sbjct: 63  PSTKK-FEAVVLEKPWEHFKSLTSFKPMPMLGGWKPIEDPKEKHVMEIGQFAVTEYNKRS 122

Query: 127 Y-CLTLISVLKCESQVVAGINYRLVLLAKNDKNHEGKYETVVWEKIWEHFRKLISFKPLL 176
              L   SV K E+QVV+G NYRL+L+ K+  + + K+E VVWEK WEHF+ L SFKP++
Sbjct: 123 KGALKFESVEKGETQVVSGTNYRLILVVKDGPSTK-KFEAVVWEKPWEHFKSLTSFKPMV 180

[Close]
BLAST of Lsi01G011640 vs. NCBI nr
Match: gi|568834137|ref|XP_006471203.1| (PREDICTED: cysteine proteinase inhibitor 5-like [Citrus sinensis])

HSP 1 Score: 147.1 bits (370), Expect = 2.9e-32
Identity = 83/177 (46.89%), Postives = 114/177 (64.41%), Query Frame = 1 

Query: 7   GGYGPCEDPEGPHIKEIAEWAVAEYNKGGH-CLTLIYVLSCESQVVAGVNYRLVLLVKDE 66
           GG+ P +DP+  H+ EI ++AV EYNK     L    V   E+QVV+G NYRL+L+VKD 
Sbjct: 30  GGWMPIKDPKEKHVMEIGQFAVTEYNKRSKSALKFESVEEGETQVVSGTNYRLILVVKDG 89

Query: 67  NNHEKVYETVVWEKIWENFRELIYFKPY------VDPESPHVKEIAEWAVAEYNKGGY-C 126
            + +K +E VV+EK WE+F+ L  FKP       +DP   HV EI ++AV EYNK     
Sbjct: 90  PSTKK-FEAVVFEKQWEHFKSLTSFKPMFGGWTPIDPTEKHVMEIGQFAVTEYNKRSKSA 149

Query: 127 LTLISVLKCESQVVAGINYRLVLLAKNDKNHEGKYETVVWEKIWEHFRKLISFKPLL 176
           L   +V K  +QVV+G NYRL+L+ K+  + + K+E VVWEK WEHF+ L SFKP++
Sbjct: 150 LKFENVEKAWTQVVSGTNYRLILVVKDGPSTK-KFEAVVWEKPWEHFKSLTSFKPMV 204

The following BLAST results are available for this feature:
BLAST of Lsi01G011640 vs. Swiss-Prot
Match NameE-valueIdentityDescription
CYTM_SOLTU7.8e-2232.43Multicystatin OS=Solanum tuberosum PE=1 SV=1[more]
CYT5_ARATH2.8e-1142.53Cysteine proteinase inhibitor 5 OS=Arabidopsis thaliana GN=CYS5 PE=2 SV=2[more]
CYT7_ORYSJ4.7e-1146.43Putative cysteine proteinase inhibitor 7 OS=Oryza sativa subsp. japonica GN=Os03... [more]
CYT1_ACTDE6.2e-1146.43Cysteine proteinase inhibitor 1 OS=Actinidia deliciosa PE=1 SV=1[more]
CYT8_ORYSJ1.5e-0941.38Cysteine proteinase inhibitor 8 OS=Oryza sativa subsp. japonica GN=Os03g0429000 ... [more]
BLAST of Lsi01G011640 vs. TrEMBL
Match NameE-valueIdentityDescription
V4T622_9ROSI6.8e-3347.78Cysteine proteinase inhibitor OS=Citrus clementina GN=CICLE_v10002632mg PE=3 SV=... [more]
O80389_CUCSA3.5e-2972.53Cysteine proteinase inhibitor OS=Cucumis sativus PE=2 SV=1[more]
A0A078EQE2_BRANA2.4e-2541.18Cysteine proteinase inhibitor OS=Brassica napus GN=BnaA09g19480D PE=3 SV=1[more]
A0A0A0KIY5_CUCSA3.5e-2155.91Cysteine proteinase inhibitor OS=Cucumis sativus GN=Csa_6G483248 PE=3 SV=1[more]
M0U917_MUSAM1.3e-2050.39Cysteine proteinase inhibitor OS=Musa acuminata subsp. malaccensis PE=3 SV=1[more]
BLAST of Lsi01G011640 vs. TAIR10
Match NameE-valueIdentityDescription
AT5G47550.11.6e-1242.53 Cystatin/monellin superfamily protein[more]
AT3G12490.21.4e-0829.41 cystatin B[more]
AT4G16500.11.8e-0837.65 Cystatin/monellin superfamily protein[more]
AT5G05110.18.9e-0846.05 Cystatin/monellin family protein[more]
AT5G12140.13.7e-0642.47 cystatin-1[more]
BLAST of Lsi01G011640 vs. NCBI nr
Match NameE-valueIdentityDescription
gi|695018835|ref|XP_009396392.1|8.9e-3451.67PREDICTED: cysteine proteinase inhibitor 1 {ECO:0000303|PubMed:14697268}-like [M... [more]
gi|568825922|ref|XP_006467325.1|3.4e-3348.30PREDICTED: multicystatin [Citrus sinensis][more]
gi|568837934|ref|XP_006472972.1|3.4e-3347.75PREDICTED: multicystatin-like, partial [Citrus sinensis][more]
gi|567878425|ref|XP_006431771.1|9.8e-3347.78hypothetical protein CICLE_v10002632mg [Citrus clementina][more]
gi|568834137|ref|XP_006471203.1|2.9e-3246.89PREDICTED: cysteine proteinase inhibitor 5-like [Citrus sinensis][more]
Annotated Terms
The following terms have been associated with this gene:
Vocabulary: Molecular Function
TermDefinition
GO:0004869cysteine-type endopeptidase inhibitor activity
Vocabulary: INTERPRO
TermDefinition
IPR027214Cystatin
IPR020381Proteinase inhibitor I25, cystatin, conserved region
IPR000010Cystatin_dom
GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0010951 negative regulation of endopeptidase activity
biological_process GO:0010466 negative regulation of peptidase activity
biological_process GO:0008150 biological_process
cellular_component GO:0005575 cellular_component
molecular_function GO:0004869 cysteine-type endopeptidase inhibitor activity
molecular_function GO:0030414 peptidase inhibitor activity
Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Lsi01G011640.1Lsi01G011640.1mRNA


InterPro
Analysis Name: InterPro Annotations of Lagenaria siceraria
Date Performed: 2017-09-18
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR000010Cystatin domainSMARTSM00043CY_4coord: 94..174
score: 5.2E-6coord: 4..93
score: 7.
IPR020381Proteinase inhibitor I25, cystatin, conserved regionPRODOMPD001231coord: 100..171
score: 4.0E-24coord: 19..90
score: 3.0
IPR020381Proteinase inhibitor I25, cystatin, conserved regionPFAMPF16845SQAPIcoord: 97..173
score: 1.5E-26coord: 16..92
score: 5.7
IPR027214CystatinPANTHERPTHR11413CYSTATIN FAMILY MEMBERcoord: 4..97
score: 2.6E-27coord: 147..176
score: 2.6
NoneNo IPR availableGENE3DG3DSA:3.10.450.10coord: 95..173
score: 1.5E-19coord: 5..92
score: 1.5
NoneNo IPR availablePANTHERPTHR11413:SF26CYSTATIN Ccoord: 4..97
score: 2.6E-27coord: 147..176
score: 2.6
NoneNo IPR availableunknownSSF54403Cystatin/monellincoord: 97..174
score: 5.74E-20coord: 4..92
score: 5.32

Synteny
The following gene(s) are orthologous to this gene:
GeneOrthologueOrganismBlock
Lsi01G011640Csa6G459990Cucumber (Chinese Long) v2culsiB431
Lsi01G011640Csa6G482230Cucumber (Chinese Long) v2culsiB434
Lsi01G011640CSPI06G25850Wild cucumber (PI 183967)cpilsiB440
Lsi01G011640CSPI06G25970Wild cucumber (PI 183967)cpilsiB436
Lsi01G011640CSPI06G26300Wild cucumber (PI 183967)cpilsiB439
Lsi01G011640Cucsa.083990Cucumber (Gy14) v1cgylsiB087
Lsi01G011640Cucsa.100800Cucumber (Gy14) v1cgylsiB125
Lsi01G011640MELO3C008065Melon (DHL92) v3.5.1lsimeB150
Lsi01G011640ClCG01G015280Watermelon (Charleston Gray)lsiwcgB127
Lsi01G011640MELO3C033110.2Melon (DHL92) v3.6.1lsimedB157
Lsi01G011640Cla97C01G014240Watermelon (97103) v2lsiwmbB115
Lsi01G011640Bhi03G001029Wax gourdlsiwgoB183
Lsi01G011640Cp4.1LG19g04630Cucurbita pepo (Zucchini)cpelsiB396
Lsi01G011640CsaV3_6G041540Cucumber (Chinese Long) v3cuclsiB462
The following gene(s) are paralogous to this gene:

None

The following block(s) are covering this gene:
GeneOrganismBlock
Lsi01G011640Cucumber (Chinese Long) v3cuclsiB021
Lsi01G011640Watermelon (97103) v2lsiwmbB114
Lsi01G011640Cucurbita maxima (Rimu)cmalsiB597
Lsi01G011640Cucurbita maxima (Rimu)cmalsiB786
Lsi01G011640Cucurbita moschata (Rifu)cmolsiB582
Lsi01G011640Cucurbita moschata (Rifu)cmolsiB774
Lsi01G011640Cucurbita pepo (Zucchini)cpelsiB054
Lsi01G011640Wild cucumber (PI 183967)cpilsiB020
Lsi01G011640Cucumber (Chinese Long) v2culsiB021
Lsi01G011640Watermelon (97103) v1lsiwmB142
Lsi01G011640Cucumber (Gy14) v2cgyblsiB017
Lsi01G011640Cucumber (Gy14) v2cgyblsiB398
Lsi01G011640Silver-seed gourdcarlsiB214
Lsi01G011640Silver-seed gourdcarlsiB742