Csa6G482230 (gene) Cucumber (Chinese Long) v2

Overview
NameCsa6G482230
Typegene
OrganismCucumis. sativus (Cucumber (Chinese Long) v2)
DescriptionCysteine proteinase inhibitor; contains IPR000010 (Proteinase inhibitor I25, cystatin)
LocationChr6 : 22084273 .. 22084759 (+)
Genome Viewer
Gene Expression
   



Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: five_prime_UTRCDSthree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
AGCATTCCATAAGTAATAGAGATTTGGGTGTGTATCAAAAGAAAGTAAGGTAGGAAAATGTGTTCTCATGTAACAACTGGAGCTTATGGTCCGTGTGAGGATCCAAATAGTTTACACGTGAAAAGAATAGCAGAATGGGCAGTGGAAGAATACAACAAAGGAGACCATTTCCTGAGCCTTGTAAGTATATTGAAATGTGAGTCGCAAGTGGTGAATGGAACCAATTGGCGTCTTAAATTGAAGTGTGTAGATCAAATTAACAATCGTTTGGGAATCTATGTGACTGTTGTGTGGGAGAAATTGGACGGAAGCCTGGTGCTCAGTGACTTCGTTCCTCTCCTCAAATGAATAAACCAACTCCTTACTTAAACTTACTGCTTTTATGCATATGTAAGTTATGTACTTCCCCTTCTTAATGATCAATATATAGATCTATAAATAATGGGATTATAAAAATAAATGAAGTAATATCCAATCATATATACAC

mRNA sequence

ATGTGTTCTCATGTAACAACTGGAGCTTATGGTCCGTGTGAGGATCCAAATAGTTTACACGTGAAAAGAATAGCAGAATGGGCAGTGGAAGAATACAACAAAGGAGACCATTTCCTGAGCCTTGTAAGTATATTGAAATGTGAGTCGCAAGTGGTGAATGGAACCAATTGGCGTCTTAAATTGAAGTGTGTAGATCAAATTAACAATCGTTTGGGAATCTATGTGACTGTTGTGTGGGAGAAATTGGACGGAAGCCTGGTGCTCAGTGACTTCGTTCCTCTCCTCAAATGA

Coding sequence (CDS)

ATGTGTTCTCATGTAACAACTGGAGCTTATGGTCCGTGTGAGGATCCAAATAGTTTACACGTGAAAAGAATAGCAGAATGGGCAGTGGAAGAATACAACAAAGGAGACCATTTCCTGAGCCTTGTAAGTATATTGAAATGTGAGTCGCAAGTGGTGAATGGAACCAATTGGCGTCTTAAATTGAAGTGTGTAGATCAAATTAACAATCGTTTGGGAATCTATGTGACTGTTGTGTGGGAGAAATTGGACGGAAGCCTGGTGCTCAGTGACTTCGTTCCTCTCCTCAAATGA

Protein sequence

MCSHVTTGAYGPCEDPNSLHVKRIAEWAVEEYNKGDHFLSLVSILKCESQVVNGTNWRLKLKCVDQINNRLGIYVTVVWEKLDGSLVLSDFVPLLK*
Homology
[Close]
BLAST of Csa6G482230 vs. TrEMBL
Match: A0A0A0KHN6_CUCSA (Uncharacterized protein OS=Cucumis sativus GN=Csa_6G482230 PE=4 SV=1)

HSP 1 Score: 203.4 bits (516), Expect = 1.3e-49
Identity = 96/96 (100.00%), Postives = 96/96 (100.00%), Query Frame = 1 

Query: 1  MCSHVTTGAYGPCEDPNSLHVKRIAEWAVEEYNKGDHFLSLVSILKCESQVVNGTNWRLK 60
          MCSHVTTGAYGPCEDPNSLHVKRIAEWAVEEYNKGDHFLSLVSILKCESQVVNGTNWRLK
Sbjct: 1  MCSHVTTGAYGPCEDPNSLHVKRIAEWAVEEYNKGDHFLSLVSILKCESQVVNGTNWRLK 60

Query: 61 LKCVDQINNRLGIYVTVVWEKLDGSLVLSDFVPLLK 97
          LKCVDQINNRLGIYVTVVWEKLDGSLVLSDFVPLLK
Sbjct: 61 LKCVDQINNRLGIYVTVVWEKLDGSLVLSDFVPLLK 96

[Close]
BLAST of Csa6G482230 vs. TrEMBL
Match: O80389_CUCSA (Cysteine proteinase inhibitor OS=Cucumis sativus PE=2 SV=1)

HSP 1 Score: 104.4 bits (259), Expect = 8.1e-20
Identity = 53/76 (69.74%), Postives = 57/76 (75.00%), Query Frame = 1 

Query: 8  GAYGPCEDPNSLHVKRIAEWAVEEYNKGD-HFLSLVSILKCESQVVNGTNWRLKLKCVDQ 67
          G Y PC+DPN  HVK IAEWAV EYNK   H L+LVSILKCESQVV G NWRL LKC D+
Sbjct: 7  GGYVPCKDPNDPHVKDIAEWAVAEYNKSQGHHLTLVSILKCESQVVAGVNWRLVLKCKDE 66

Query: 68 INNRLGIYVTVVWEKL 83
           NN  G Y TVVWEK+
Sbjct: 67 -NNGEGNYETVVWEKI 81

[Close]
BLAST of Csa6G482230 vs. TrEMBL
Match: A0A0A0KHK7_CUCSA (Cysteine proteinase inhibitor OS=Cucumis sativus GN=Csa_6G459990 PE=3 SV=1)

HSP 1 Score: 95.5 bits (236), Expect = 3.8e-17
Identity = 50/85 (58.82%), Postives = 59/85 (69.41%), Query Frame = 1 

Query: 1  MCSHVTTGAYGPCEDPNSLHVKRIAEWAVEEYN-KGDH---FLSLVSILKCESQVVNGTN 60
          M SHVT GAYGPCE+ +  H K +A+WAV EYN K  H   +L L+S+LKCESQVV GTN
Sbjct: 1  MSSHVTLGAYGPCENSDGEHAKEVAQWAVTEYNIKHRHERPYLYLLSVLKCESQVVAGTN 60

Query: 61 WRLKLKCVDQINNRLGIYVTVVWEK 82
          WRL LKC D+ N  +     VVWEK
Sbjct: 61 WRLGLKCKDENNIEVNC-EAVVWEK 84

[Close]
BLAST of Csa6G482230 vs. TrEMBL
Match: A0A0A0KF17_CUCSA (Cysteine proteinase inhibitor OS=Cucumis sativus GN=Csa_6G460000 PE=3 SV=1)

HSP 1 Score: 87.0 bits (214), Expect = 1.3e-14
Identity = 50/97 (51.55%), Postives = 61/97 (62.89%), Query Frame = 1 

Query: 1  MCSHVTTGAYGPCEDPNSLHVKRIAEWAVEEYN-KGDH---FLSLVSILKCESQVVNGTN 60
          M S    G YGPCE+ +  H K +A+WAV EYN K  H   +L L+S+LKCESQVV GTN
Sbjct: 1  MSSDSIKGGYGPCENSDGEHAKEVAQWAVTEYNIKHRHERPYLYLLSVLKCESQVVAGTN 60

Query: 61 WRLKLKCVDQINNRLGIYVTVVWEK-LDGSLVLSDFV 93
          WRL LKC D+ N  +     VVWEK  +  L L+ FV
Sbjct: 61 WRLGLKCKDENNIEVNC-EAVVWEKRWENFLELTSFV 96

[Close]
BLAST of Csa6G482230 vs. TrEMBL
Match: A0A0A0KIY5_CUCSA (Cysteine proteinase inhibitor OS=Cucumis sativus GN=Csa_6G483248 PE=3 SV=1)

HSP 1 Score: 77.0 bits (188), Expect = 1.4e-11
Identity = 41/76 (53.95%), Postives = 50/76 (65.79%), Query Frame = 1 

Query: 8  GAYGPCEDPNSLHVKRIAEWAVEEYNK--GDHFLSLVSILKCESQVVNGTNWRLKLKCVD 67
          G Y P +DP SL +K +AEWAVEEYNK  G H L  V IL CESQ+V G N+R  L+  D
Sbjct: 10 GGYTPVKDPQSLRMKELAEWAVEEYNKKEGTH-LRFVCILVCESQIVEGVNYRFILRVKD 69

Query: 68 QINNRLGIYVTVVWEK 82
          + N+  G Y  +VWEK
Sbjct: 70 E-NDNEGNYEAIVWEK 83

[Close]
BLAST of Csa6G482230 vs. TAIR10
Match: AT5G47550.1 (AT5G47550.1 Cystatin/monellin superfamily protein)

HSP 1 Score: 47.0 bits (110), Expect = 7.8e-06
Identity = 28/78 (35.90%), Postives = 42/78 (53.85%), Query Frame = 1 

Query: 8   GAYGPCEDPNSLHVKRIAEWAVEEYNK-GDHFLSLVSILKCESQVVNGTNWRLKLKCVDQ 67
           G + P  +     V  I E+AV EYNK  +  L   +++  E+QVV+GTN+RLK+     
Sbjct: 29  GGWSPISNVTDPQVVEIGEFAVSEYNKRSESGLKFETVVSGETQVVSGTNYRLKVAA--- 88

Query: 68  INNRLGI---YVTVVWEK 82
            N+  G+   Y+ +VW+K
Sbjct: 89  -NDGDGVSKNYLAIVWDK 102

[Close]
BLAST of Csa6G482230 vs. NCBI nr
Match: gi|700193130|gb|KGN48334.1| (hypothetical protein Csa_6G482230 [Cucumis sativus])

HSP 1 Score: 203.4 bits (516), Expect = 1.8e-49
Identity = 96/96 (100.00%), Postives = 96/96 (100.00%), Query Frame = 1 

Query: 1  MCSHVTTGAYGPCEDPNSLHVKRIAEWAVEEYNKGDHFLSLVSILKCESQVVNGTNWRLK 60
          MCSHVTTGAYGPCEDPNSLHVKRIAEWAVEEYNKGDHFLSLVSILKCESQVVNGTNWRLK
Sbjct: 1  MCSHVTTGAYGPCEDPNSLHVKRIAEWAVEEYNKGDHFLSLVSILKCESQVVNGTNWRLK 60

Query: 61 LKCVDQINNRLGIYVTVVWEKLDGSLVLSDFVPLLK 97
          LKCVDQINNRLGIYVTVVWEKLDGSLVLSDFVPLLK
Sbjct: 61 LKCVDQINNRLGIYVTVVWEKLDGSLVLSDFVPLLK 96

[Close]
BLAST of Csa6G482230 vs. NCBI nr
Match: gi|525507274|ref|NP_001267677.1| (cysteine proteinase inhibitor 5-like [Cucumis sativus])

HSP 1 Score: 104.4 bits (259), Expect = 1.2e-19
Identity = 53/76 (69.74%), Postives = 57/76 (75.00%), Query Frame = 1 

Query: 8  GAYGPCEDPNSLHVKRIAEWAVEEYNKGD-HFLSLVSILKCESQVVNGTNWRLKLKCVDQ 67
          G Y PC+DPN  HVK IAEWAV EYNK   H L+LVSILKCESQVV G NWRL LKC D+
Sbjct: 7  GGYVPCKDPNDPHVKDIAEWAVAEYNKSQGHHLTLVSILKCESQVVAGVNWRLVLKCKDE 66

Query: 68 INNRLGIYVTVVWEKL 83
           NN  G Y TVVWEK+
Sbjct: 67 -NNGEGNYETVVWEKI 81

[Close]
BLAST of Csa6G482230 vs. NCBI nr
Match: gi|700193100|gb|KGN48304.1| (hypothetical protein Csa_6G459990 [Cucumis sativus])

HSP 1 Score: 95.5 bits (236), Expect = 5.4e-17
Identity = 50/85 (58.82%), Postives = 59/85 (69.41%), Query Frame = 1 

Query: 1  MCSHVTTGAYGPCEDPNSLHVKRIAEWAVEEYN-KGDH---FLSLVSILKCESQVVNGTN 60
          M SHVT GAYGPCE+ +  H K +A+WAV EYN K  H   +L L+S+LKCESQVV GTN
Sbjct: 1  MSSHVTLGAYGPCENSDGEHAKEVAQWAVTEYNIKHRHERPYLYLLSVLKCESQVVAGTN 60

Query: 61 WRLKLKCVDQINNRLGIYVTVVWEK 82
          WRL LKC D+ N  +     VVWEK
Sbjct: 61 WRLGLKCKDENNIEVNC-EAVVWEK 84

[Close]
BLAST of Csa6G482230 vs. NCBI nr
Match: gi|700193101|gb|KGN48305.1| (hypothetical protein Csa_6G460000 [Cucumis sativus])

HSP 1 Score: 87.0 bits (214), Expect = 1.9e-14
Identity = 50/97 (51.55%), Postives = 61/97 (62.89%), Query Frame = 1 

Query: 1  MCSHVTTGAYGPCEDPNSLHVKRIAEWAVEEYN-KGDH---FLSLVSILKCESQVVNGTN 60
          M S    G YGPCE+ +  H K +A+WAV EYN K  H   +L L+S+LKCESQVV GTN
Sbjct: 1  MSSDSIKGGYGPCENSDGEHAKEVAQWAVTEYNIKHRHERPYLYLLSVLKCESQVVAGTN 60

Query: 61 WRLKLKCVDQINNRLGIYVTVVWEK-LDGSLVLSDFV 93
          WRL LKC D+ N  +     VVWEK  +  L L+ FV
Sbjct: 61 WRLGLKCKDENNIEVNC-EAVVWEKRWENFLELTSFV 96

[Close]
BLAST of Csa6G482230 vs. NCBI nr
Match: gi|700193138|gb|KGN48342.1| (hypothetical protein Csa_6G483248 [Cucumis sativus])

HSP 1 Score: 77.0 bits (188), Expect = 2.0e-11
Identity = 41/76 (53.95%), Postives = 50/76 (65.79%), Query Frame = 1 

Query: 8  GAYGPCEDPNSLHVKRIAEWAVEEYNK--GDHFLSLVSILKCESQVVNGTNWRLKLKCVD 67
          G Y P +DP SL +K +AEWAVEEYNK  G H L  V IL CESQ+V G N+R  L+  D
Sbjct: 10 GGYTPVKDPQSLRMKELAEWAVEEYNKKEGTH-LRFVCILVCESQIVEGVNYRFILRVKD 69

Query: 68 QINNRLGIYVTVVWEK 82
          + N+  G Y  +VWEK
Sbjct: 70 E-NDNEGNYEAIVWEK 83

The following BLAST results are available for this feature:
BLAST of Csa6G482230 vs. TrEMBL
Match NameE-valueIdentityDescription
A0A0A0KHN6_CUCSA1.3e-49100.00Uncharacterized protein OS=Cucumis sativus GN=Csa_6G482230 PE=4 SV=1[more]
O80389_CUCSA8.1e-2069.74Cysteine proteinase inhibitor OS=Cucumis sativus PE=2 SV=1[more]
A0A0A0KHK7_CUCSA3.8e-1758.82Cysteine proteinase inhibitor OS=Cucumis sativus GN=Csa_6G459990 PE=3 SV=1[more]
A0A0A0KF17_CUCSA1.3e-1451.55Cysteine proteinase inhibitor OS=Cucumis sativus GN=Csa_6G460000 PE=3 SV=1[more]
A0A0A0KIY5_CUCSA1.4e-1153.95Cysteine proteinase inhibitor OS=Cucumis sativus GN=Csa_6G483248 PE=3 SV=1[more]
BLAST of Csa6G482230 vs. TAIR10
Match NameE-valueIdentityDescription
AT5G47550.17.8e-0635.90 Cystatin/monellin superfamily protein[more]
BLAST of Csa6G482230 vs. NCBI nr
Match NameE-valueIdentityDescription
gi|700193130|gb|KGN48334.1|1.8e-49100.00hypothetical protein Csa_6G482230 [Cucumis sativus][more]
gi|525507274|ref|NP_001267677.1|1.2e-1969.74cysteine proteinase inhibitor 5-like [Cucumis sativus][more]
gi|700193100|gb|KGN48304.1|5.4e-1758.82hypothetical protein Csa_6G459990 [Cucumis sativus][more]
gi|700193101|gb|KGN48305.1|1.9e-1451.55hypothetical protein Csa_6G460000 [Cucumis sativus][more]
gi|700193138|gb|KGN48342.1|2.0e-1153.95hypothetical protein Csa_6G483248 [Cucumis sativus][more]
Annotated Terms
The following terms have been associated with this gene:
Vocabulary: INTERPRO
TermDefinition
IPR020381Proteinase inhibitor I25, cystatin, conserved region
GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0010466 negative regulation of peptidase activity
biological_process GO:0010951 negative regulation of endopeptidase activity
cellular_component GO:0005575 cellular_component
cellular_component GO:0016020 membrane
molecular_function GO:0030414 peptidase inhibitor activity
molecular_function GO:0004869 cysteine-type endopeptidase inhibitor activity
Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Csa6G482230.1Csa6G482230.1mRNA


InterPro
Analysis Name: InterPro Annotations of cucumber (Chinese Long)
Date Performed: 2016-09-28
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR020381Proteinase inhibitor I25, cystatin, conserved regionPRODOMPD001231coord: 20..82
score: 2.0
IPR020381Proteinase inhibitor I25, cystatin, conserved regionPFAMPF16845SQAPIcoord: 17..91
score: 1.9
NoneNo IPR availableGENE3DG3DSA:3.10.450.10coord: 8..81
score: 4.1
NoneNo IPR availableunknownSSF54403Cystatin/monellincoord: 4..83
score: 9.78

Synteny
The following gene(s) are orthologous to this gene:
GeneOrthologueOrganismBlock
Csa6G482230Lsi01G011640Bottle gourd (USVL1VR-Ls)culsiB434
Csa6G482230Bhi03G001029Wax gourdcuwgoB622
Csa6G482230CSPI06G26190Wild cucumber (PI 183967)cpicuB313
Csa6G482230Cucsa.100800Cucumber (Gy14) v1cgycuB121
Csa6G482230CsGy6G025710Cucumber (Gy14) v2cgybcuB281
The following gene(s) are paralogous to this gene:

None

The following block(s) are covering this gene:
GeneOrganismBlock
Csa6G482230Cucurbita maxima (Rimu)cmacuB681
Csa6G482230Cucurbita moschata (Rifu)cmocuB670
Csa6G482230Cucurbita moschata (Rifu)cmocuB865
Csa6G482230Melon (DHL92) v3.5.1cumeB508
Csa6G482230Watermelon (Charleston Gray)cuwcgB432
Csa6G482230Watermelon (97103) v1cuwmB502
Csa6G482230Cucurbita pepo (Zucchini)cpecuB085
Csa6G482230Cucurbita pepo (Zucchini)cpecuB503
Csa6G482230Melon (DHL92) v3.6.1cumedB487
Csa6G482230Silver-seed gourdcarcuB0267
Csa6G482230Silver-seed gourdcarcuB0826
Csa6G482230Watermelon (97103) v2cuwmbB412