Lsi01G007390 (gene) Bottle gourd (USVL1VR-Ls) | Cucurbit Genomics Database (CuGenDB)

Overview


Name	Lsi01G007390
Type	gene
Organism	Lagenaria siceraria (Bottle gourd (USVL1VR-Ls))
Description	Heavy metal transport/detoxification superfamily protein
Location	chr01 : 5828376 .. 5829651 (+)

Genome Viewer

Gene Expression

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: five_prime_UTRCDSthree_prime_UTR

Hold the cursor over a type above to highlight its positions in the sequence below.

TTTTAAAGTAAATGCAACGTCCACAGCAACAAAAACCAAGCTTCCTAACCCAAATTGTTTTTGGTGATTGGGGGGCCACAACCAAAACACAACCCTTTTCCTTCGTTTTTTAAATCTAACCCAAATGGCTATATAATGAGGTCTGTTTCCTTTCATATAACCATCTCAAAACCAGAGCTAGAGACAGAGAAAGCAGCCTTTGTTTTTAATGTCCTTTTCTTTGCTTGTTTGCTTGAAGGGAAGGTAAAGAAAAAGATCCATAATTTCTAGCCAAAGTTACATTGCCATTCTTTCTACAAACTCAATTTGACTCGAAAGTTTCTCTGGGTTTTGCTCCTGTGAGCTTCTGCTGTACCTTTCCTTTTTCAACTACATCGATTAAAGTGTGAAAATGGGTGTTGGTGGGACTTTGGACTACTTGTCAGATTTGGTGGGTAGTAGCCATAAACACAAGAAGAAGAAACAGCTTCAAACTGTGGAGTTGAAGGTCAGAATGGACTGTGATGGCTGTGAACTTAAGGTCAAGAATGCCCTCTCTTCACTCAGTGGTATGTCTAAAGAAACTCACCCCTTTTGTTTTTACTTGTTACTGTTTTCTCCATCTAACAAAATGCAGTGTTTTTTCTAACTTTGAAGGGGTGAAATCTGTGGAGATAAACAGAAAGCAGCAGAAGGTGACAGTTACAGGGTATGTTGAAGCAAGCAAAGTACTGAAGAAGGCAAAGTCTACAGGGAAGAAGGCTGAGATATGGCCATATGTTCCCTATAGCTTAGTGTCACAGCCTTACATTGCTCAGGCTTATGACAAGAAGGCACCACCAGGGTATGTAAGAAATGTGGAGCAAACTGCCACTACTGCTTCTGTTACCAAATATGAAGATCCTTACATCAACATGTTCAGTGATGACAACCCAAATGCCTGCTCTATTATGTAATTCTTTTCTGCTAAAACAAGTCTCCTGTTTTGTACCTCTCTCTATATCACTCTTCTGCTAAATTTTTGTCAAACAGCTCAATAATTCTGGTAATGATATATATTACTGTGTGTTTCAATCATTTGTGAAGAAGAAAGGGTCAACTTGGGGGATGAGTAATTAGAATATTTGGGGTTGTTAGTTGTTAATAGCTGATGTAAGATATTACTTTATTTCTGCTGTTGGTTTAAGAGGACGCATGATATTGTAATTTCCTGTTTGTGAGACGACTTTGGCTTCTCCACAGCTGTTCTTTCACCTAAAAAAAACAAGATTCCAACCTGGTTATTAACTGTTAGAGG

mRNA sequence

TTTTAAAGTAAATGCAACGTCCACAGCAACAAAAACCAAGCTTCCTAACCCAAATTGTTTTTGGTGATTGGGGGGCCACAACCAAAACACAACCCTTTTCCTTCGTTTTTTAAATCTAACCCAAATGGCTATATAATGAGGTCTGTTTCCTTTCATATAACCATCTCAAAACCAGAGCTAGAGACAGAGAAAGCAGCCTTTGTTTTTAATGTCCTTTTCTTTGCTTGTTTGCTTGAAGGGAAGGTAAAGAAAAAGATCCATAATTTCTAGCCAAAGTTACATTGCCATTCTTTCTACAAACTCAATTTGACTCGAAAGTTTCTCTGGGTTTTGCTCCTGTGAGCTTCTGCTGTACCTTTCCTTTTTCAACTACATCGATTAAAGTGTGAAAATGGGTGTTGGTGGGACTTTGGACTACTTGTCAGATTTGGTGGGTAGTAGCCATAAACACAAGAAGAAGAAACAGCTTCAAACTGTGGAGTTGAAGGTCAGAATGGACTGTGATGGCTGTGAACTTAAGGTCAAGAATGCCCTCTCTTCACTCAGTGGGGTGAAATCTGTGGAGATAAACAGAAAGCAGCAGAAGGTGACAGTTACAGGGTATGTTGAAGCAAGCAAAGTACTGAAGAAGGCAAAGTCTACAGGGAAGAAGGCTGAGATATGGCCATATGTTCCCTATAGCTTAGTGTCACAGCCTTACATTGCTCAGGCTTATGACAAGAAGGCACCACCAGGGTATGTAAGAAATGTGGAGCAAACTGCCACTACTGCTTCTGTTACCAAATATGAAGATCCTTACATCAACATGTTCAGTGATGACAACCCAAATGCCTGCTCTATTATGTAATTCTTTTCTGCTAAAACAAGTCTCCTGTTTTGTACCTCTCTCTATATCACTCTTCTGCTAAATTTTTGTCAAACAGCTCAATAATTCTGGTAATGATATATATTACTGTGTGTTTCAATCATTTGTGAAGAAGAAAGGGTCAACTTGGGGGATGAGTAATTAGAATATTTGGGGTTGTTAGTTGTTAATAGCTGATGTAAGATATTACTTTATTTCTGCTGTTGGTTTAAGAGGACGCATGATATTGTAATTTCCTGTTTGTGAGACGACTTTGGCTTCTCCACAGCTGTTCTTTCACCTAAAAAAAACAAGATTCCAACCTGGTTATTAACTGTTAGAGG

Coding sequence (CDS)

ATGGGTGTTGGTGGGACTTTGGACTACTTGTCAGATTTGGTGGGTAGTAGCCATAAACACAAGAAGAAGAAACAGCTTCAAACTGTGGAGTTGAAGGTCAGAATGGACTGTGATGGCTGTGAACTTAAGGTCAAGAATGCCCTCTCTTCACTCAGTGGGGTGAAATCTGTGGAGATAAACAGAAAGCAGCAGAAGGTGACAGTTACAGGGTATGTTGAAGCAAGCAAAGTACTGAAGAAGGCAAAGTCTACAGGGAAGAAGGCTGAGATATGGCCATATGTTCCCTATAGCTTAGTGTCACAGCCTTACATTGCTCAGGCTTATGACAAGAAGGCACCACCAGGGTATGTAAGAAATGTGGAGCAAACTGCCACTACTGCTTCTGTTACCAAATATGAAGATCCTTACATCAACATGTTCAGTGATGACAACCCAAATGCCTGCTCTATTATGTAA

Protein sequence

MGVGGTLDYLSDLVGSSHKHKKKKQLQTVELKVRMDCDGCELKVKNALSSLSGVKSVEINRKQQKVTVTGYVEASKVLKKAKSTGKKAEIWPYVPYSLVSQPYIAQAYDKKAPPGYVRNVEQTATTASVTKYEDPYINMFSDDNPNACSIM

Homology

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BLAST of Lsi01G007390 vs. Swiss-Prot
Match: HIP20_ARATH (Heavy metal-associated isoprenylated plant protein 20 OS=Arabidopsis thaliana GN=HIPP20 PE=2 SV=1)

HSP 1 Score: 179.5 bits (454), Expect = 2.8e-44
Identity = 85/144 (59.03%), Postives = 110/144 (76.39%), Query Frame = 1


Query: 8   DYLSDLVGSSHKHKKKKQLQTVELKVRMDCDGCELKVKNALSSLSGVKSVEINRKQQKVT 67
           +Y+SD    + K +K+K +QTV +KV+MDCDGCE +VKNA+SS+ GVKSVE+NRK  KVT
Sbjct: 9   EYISDYFRVTRKRRKRKVMQTVNIKVKMDCDGCERRVKNAVSSMKGVKSVEVNRKIHKVT 68

Query: 68  VTGYVEASKVLKKAKSTGKKAEIWPYVPYSLVSQPYIAQAYDKKAPPGYVRNVEQTATTA 127
           V+GYVE  KVLK+ + TGKKAEIWPYVPY++V+ PY    YDKKAP GYVR  EQ+    
Sbjct: 69  VSGYVEPKKVLKRIERTGKKAEIWPYVPYNMVAYPYAVGTYDKKAPAGYVRKSEQSQLQL 128

Query: 128 SVTKYEDPYINMFSDDNPNACSIM 152
                E+ YI++FSD+NPNAC++M
Sbjct: 129 LPGAPENHYISLFSDENPNACTVM 152

[Close]

BLAST of Lsi01G007390 vs. Swiss-Prot
Match: HIP22_ARATH (Heavy metal-associated isoprenylated plant protein 22 OS=Arabidopsis thaliana GN=HIPP22 PE=2 SV=1)

HSP 1 Score: 172.9 bits (437), Expect = 2.6e-42
Identity = 86/153 (56.21%), Postives = 113/153 (73.86%), Query Frame = 1


Query: 5   GTLDYLSDLVG-----SSHKHKKKKQLQTVELKVRMDCDGCELKVKNALSSLSGVKSVEI 64
           G L+YLS+        S  K KK+K +QTV +KV++DCDGCE K+KNA+SS+ G KSVE+
Sbjct: 2   GALNYLSEYFSNHFYVSIRKRKKRKVMQTVNIKVKIDCDGCERKIKNAVSSIKGAKSVEV 61

Query: 65  NRKQQKVTVTGYVEASKVLKKAKSTG-KKAEIWPYVPYSLVSQPYIAQAYDKKAPPGYVR 124
           NRK  KVTV+GYV+  KVLK  +STG KKAE+WPYVPY++V+ PY A AYDK+APPG+VR
Sbjct: 62  NRKMHKVTVSGYVDPKKVLKTVQSTGKKKAELWPYVPYTMVAYPYAAGAYDKRAPPGFVR 121

Query: 125 NVEQTATTASVTKYEDPYINMFSDDNPNACSIM 152
             EQ       T  +D  +++FSD+NPNAC++M
Sbjct: 122 KSEQAQAQPGST--DDKLMSLFSDENPNACTVM 152

[Close]

BLAST of Lsi01G007390 vs. Swiss-Prot
Match: HIP21_ARATH (Heavy metal-associated isoprenylated plant protein 21 OS=Arabidopsis thaliana GN=HIPP21 PE=2 SV=1)

HSP 1 Score: 155.6 bits (392), Expect = 4.3e-37
Identity = 76/150 (50.67%), Postives = 108/150 (72.00%), Query Frame = 1


Query: 5   GTLDYLSDLVGSSHKHKK--KKQLQTVELKVRMDCDGCELKVKNALSSLSGVKSVEINRK 64
           G  DY+S     ++ + K  +K LQTV++KV+MDCDGCE +V+N +  + GVKSVE+NRK
Sbjct: 2   GAFDYISSFCSYTYANAKTKRKPLQTVDIKVKMDCDGCERRVRNVVRRMKGVKSVEVNRK 61

Query: 65  QQKVTVTGYVEASKVLKKAKSTGKKAEIWPYVPYSLVSQPYIAQAYDKKAPPGYVRNVEQ 124
           Q ++TV G+V+ +KVLK+ KSTGKKAE WPY+P  +V  P+    YDK+AP G++RN  Q
Sbjct: 62  QSRITVNGHVDPNKVLKRVKSTGKKAEFWPYIPQHMVYYPFAPGMYDKRAPAGHIRNPTQ 121

Query: 125 TATTASVTKYEDPYINMFSDDNPN-ACSIM 152
           +  TA+    E+ Y+++FSDDN + ACSIM
Sbjct: 122 SFPTANAP--EENYVSLFSDDNVHAACSIM 149

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BLAST of Lsi01G007390 vs. Swiss-Prot
Match: HIP26_ARATH (Heavy metal-associated isoprenylated plant protein 26 OS=Arabidopsis thaliana GN=HIPP26 PE=1 SV=1)

HSP 1 Score: 152.5 bits (384), Expect = 3.6e-36
Identity = 77/152 (50.66%), Postives = 103/152 (67.76%), Query Frame = 1


Query: 5   GTLDYLSDLVGSSHKHK--KKKQLQTVELKVRMDCDGCELKVKNALSSLSGVKSVEINRK 64
           G LD++S++   SH HK  K+KQLQTVE+KV+MDC+GCE KV+ ++  + GV SV +  K
Sbjct: 2   GVLDHVSEMFDCSHGHKIKKRKQLQTVEIKVKMDCEGCERKVRRSVEGMKGVSSVTLEPK 61

Query: 65  QQKVTVTGYVEASKVL-KKAKSTGKKAEIWPYVPYSLVSQPYIAQAYDKKAPPGYVRNVE 124
             KVTV GYV+ +KV+ + +  TGKK E+WPYVPY +V+ PY A  YDKKAP GYVR V+
Sbjct: 62  AHKVTVVGYVDPNKVVARMSHRTGKKVELWPYVPYDVVAHPYAAGVYDKKAPSGYVRRVD 121

Query: 125 QTATT--ASVTKYEDPYINMFSDDNPNACSIM 152
               +  A  +  E  Y   FSD+NP AC +M
Sbjct: 122 DPGVSQLARASSTEVRYTTAFSDENPAACVVM 153

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BLAST of Lsi01G007390 vs. Swiss-Prot
Match: HIP27_ARATH (Heavy metal-associated isoprenylated plant protein 27 OS=Arabidopsis thaliana GN=HIPP27 PE=1 SV=1)

HSP 1 Score: 147.9 bits (372), Expect = 8.9e-35
Identity = 73/136 (53.68%), Postives = 94/136 (69.12%), Query Frame = 1


Query: 20  HKKKKQLQTVELKVRMDCDGCELKVKNALSSLSGVKSVEINRKQQKVTVTGYVEASKVLK 79
           HKK KQ Q VE+KV+MDC+GCE +V+ ++  + GV  V ++ KQ K+TV G+V+ SKV+ 
Sbjct: 12  HKKLKQFQKVEIKVKMDCEGCERRVRKSVEGMKGVSKVTVDPKQSKLTVEGFVQPSKVVH 71

Query: 80  KA-KSTGKKAEIWPYVPYSLVSQPYIAQAYDKKAPPGYVRNV---EQTATTASVTKYEDP 139
           +    TGKKAE+WPYVPY +V  PY   AYDKKAPPGYVRN       A  A  + +E  
Sbjct: 72  RVMHRTGKKAELWPYVPYEVVPHPYAPGAYDKKAPPGYVRNALADPLVAPLARASSFEVK 131

Query: 140 YINMFSDDNPNACSIM 152
           Y + FSDDNPNAC+IM
Sbjct: 132 YTSAFSDDNPNACTIM 147

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BLAST of Lsi01G007390 vs. TrEMBL
Match: A0A0A0KIY2_CUCSA (Uncharacterized protein OS=Cucumis sativus GN=Csa_6G500660 PE=4 SV=1)

HSP 1 Score: 297.0 bits (759), Expect = 1.3e-77
Identity = 146/151 (96.69%), Postives = 151/151 (100.00%), Query Frame = 1


Query: 1   MGVGGTLDYLSDLVGSSHKHKKKKQLQTVELKVRMDCDGCELKVKNALSSLSGVKSVEIN 60
           MGVGGTL+YLSDLVG++HKHKKKKQLQTVELKVRMDCDGCELKVKNALSSLSGVKSVEIN
Sbjct: 1   MGVGGTLEYLSDLVGNTHKHKKKKQLQTVELKVRMDCDGCELKVKNALSSLSGVKSVEIN 60

Query: 61  RKQQKVTVTGYVEASKVLKKAKSTGKKAEIWPYVPYSLVSQPYIAQAYDKKAPPGYVRNV 120
           RKQQKVTVTGYVEASK+LKKAKSTGKKAEIWPYVPYSLVSQPYIAQAYDKKAPPGYVRNV
Sbjct: 61  RKQQKVTVTGYVEASKILKKAKSTGKKAEIWPYVPYSLVSQPYIAQAYDKKAPPGYVRNV 120

Query: 121 EQTATTASVTKYEDPYINMFSDDNPNACSIM 152
           EQTATTASVTKYEDPYINMFSDDNPNACS+M
Sbjct: 121 EQTATTASVTKYEDPYINMFSDDNPNACSVM 151

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BLAST of Lsi01G007390 vs. TrEMBL
Match: B9SSL2_RICCO (Metal ion binding protein, putative OS=Ricinus communis GN=RCOM_0985130 PE=4 SV=1)

HSP 1 Score: 266.5 bits (680), Expect = 1.9e-68
Identity = 133/153 (86.93%), Postives = 146/153 (95.42%), Query Frame = 1


Query: 1   MGVGGTLDYLSDLVGSS-HKHKK-KKQLQTVELKVRMDCDGCELKVKNALSSLSGVKSVE 60
           MGVGGTL+YLSDL+GSS HK+KK KKQLQTVELK+RMDCDGCELKVKNALSS+SGVK VE
Sbjct: 1   MGVGGTLEYLSDLMGSSGHKYKKKKKQLQTVELKIRMDCDGCELKVKNALSSMSGVKKVE 60

Query: 61  INRKQQKVTVTGYVEASKVLKKAKSTGKKAEIWPYVPYSLVSQPYIAQAYDKKAPPGYVR 120
           INRKQQKVTVTGYV+ +KVLKKAKSTGKKAEIWPYVPY+LV+QPYIAQAYDKKAPPGYVR
Sbjct: 61  INRKQQKVTVTGYVDPNKVLKKAKSTGKKAEIWPYVPYNLVAQPYIAQAYDKKAPPGYVR 120

Query: 121 NVEQTATTASVTKYEDPYINMFSDDNPNACSIM 152
           NVE TAT+ +VT+YEDPY +MFSDDNPNACSIM
Sbjct: 121 NVENTATSGTVTRYEDPYSSMFSDDNPNACSIM 153

[Close]

BLAST of Lsi01G007390 vs. TrEMBL
Match: M5W0M5_PRUPE (Uncharacterized protein OS=Prunus persica GN=PRUPE_ppa012771mg PE=4 SV=1)

HSP 1 Score: 262.3 bits (669), Expect = 3.6e-67
Identity = 132/155 (85.16%), Postives = 145/155 (93.55%), Query Frame = 1


Query: 1   MGVGGTLDYLSDLVGSS-HKHKKKKQLQTVELKVRMDCDGCELKVKNALSSLSGVKSVEI 60
           MGVGGTL+YLSDLV +S HKHKKKKQLQTVELKVRMDCDGCELKVK ALSSLSGVKSVEI
Sbjct: 1   MGVGGTLEYLSDLVNNSGHKHKKKKQLQTVELKVRMDCDGCELKVKKALSSLSGVKSVEI 60

Query: 61  NRKQQKVTVTGYVEASKVLKKAKSTGKKAEIWPYVPYSLVSQPYIAQAYDKKAPPGYVRN 120
           NRKQQKV+VTGYVEA+KVLKKAKSTGK+AEIWPYVPY+LV+QPYIAQAYDK+APPG+VRN
Sbjct: 61  NRKQQKVSVTGYVEANKVLKKAKSTGKRAEIWPYVPYNLVAQPYIAQAYDKRAPPGHVRN 120

Query: 121 VE---QTATTASVTKYEDPYINMFSDDNPNACSIM 152
           VE    T++T +VT+YEDPY  MFSDDNPNACSIM
Sbjct: 121 VENMNMTSSTGTVTRYEDPYTTMFSDDNPNACSIM 155

[Close]

BLAST of Lsi01G007390 vs. TrEMBL
Match: V4S1Z4_9ROSI (Uncharacterized protein OS=Citrus clementina GN=CICLE_v10026695mg PE=4 SV=1)

HSP 1 Score: 260.8 bits (665), Expect = 1.1e-66
Identity = 133/153 (86.93%), Postives = 144/153 (94.12%), Query Frame = 1


Query: 1   MGVGGTLDYLSDLVGSS-HKHKK-KKQLQTVELKVRMDCDGCELKVKNALSSLSGVKSVE 60
           MGV GTL+YLSDL+GSS HKHKK KKQLQTV+LKVRMDCDGCELKVKNA+SSLSGVKSVE
Sbjct: 1   MGVAGTLEYLSDLMGSSGHKHKKKKKQLQTVDLKVRMDCDGCELKVKNAVSSLSGVKSVE 60

Query: 61  INRKQQKVTVTGYVEASKVLKKAKSTGKKAEIWPYVPYSLVSQPYIAQAYDKKAPPGYVR 120
           INRKQQKV+VTGYVEA+KVLKKAKSTGK+AEIWPYVPY+LV+QPYIA AYDK+APPGYVR
Sbjct: 61  INRKQQKVSVTGYVEANKVLKKAKSTGKRAEIWPYVPYNLVAQPYIAGAYDKRAPPGYVR 120

Query: 121 NVEQTATTASVTKYEDPYINMFSDDNPNACSIM 152
            VE TAT ASVT  EDPYI+MFSDDNPNACSIM
Sbjct: 121 KVEGTATNASVTTLEDPYISMFSDDNPNACSIM 153

[Close]

BLAST of Lsi01G007390 vs. TrEMBL
Match: B9H568_POPTR (Heavy-metal-associated domain-containing family protein OS=Populus trichocarpa GN=POPTR_0005s08160g PE=4 SV=1)

HSP 1 Score: 260.8 bits (665), Expect = 1.1e-66
Identity = 130/153 (84.97%), Postives = 141/153 (92.16%), Query Frame = 1


Query: 1   MGVGGTLDYLSDLVGSS-HKHKKKKQLQTVELKVRMDCDGCELKVKNALSSLSGVKSVEI 60
           MGV GTL+YLSDLVGS  HKHKKKKQLQTVELKVRMDCDGCELKVK A+SSLSGVK VEI
Sbjct: 1   MGVSGTLEYLSDLVGSGGHKHKKKKQLQTVELKVRMDCDGCELKVKKAISSLSGVKKVEI 60

Query: 61  NRKQQKVTVTGYVEASKVLKKAKSTGKKAEIWPYVPYSLVSQPYIAQAYDKKAPPGYVRN 120
           NRKQQ+VTVTGYV++SKVLKKAKSTGKKAEIWPYVPY+LV+QPY  QAYDKKAPPGYVRN
Sbjct: 61  NRKQQRVTVTGYVDSSKVLKKAKSTGKKAEIWPYVPYNLVAQPYAVQAYDKKAPPGYVRN 120

Query: 121 VEQTATTASVTKY-EDPYINMFSDDNPNACSIM 152
           VE T TT +VT+Y +DPY +MFSDDNPNACSIM
Sbjct: 121 VENTVTTGTVTRYDQDPYTSMFSDDNPNACSIM 153

[Close]

BLAST of Lsi01G007390 vs. TAIR10
Match: AT4G39700.1 (AT4G39700.1 Heavy metal transport/detoxification superfamily protein )

HSP 1 Score: 232.3 bits (591), Expect = 2.0e-61
Identity = 116/158 (73.42%), Postives = 136/158 (86.08%), Query Frame = 1


Query: 1   MGVGGTLDYLSDLVGSSHKH-----KKKKQLQTVELKVRMDCDGCELKVKNALSSLSGVK 60
           MGVGGTL+Y+S+L+G+   H     KKKKQ QTVELKVRMDCDGC LK+KN+LSSL GVK
Sbjct: 1   MGVGGTLEYISELIGNGGSHSYGKRKKKKQFQTVELKVRMDCDGCVLKIKNSLSSLKGVK 60

Query: 61  SVEINRKQQKVTVTGYVEASKVLKKAKSTGKKAEIWPYVPYSLVSQPYIAQAYDKKAPPG 120
           +VEIN+KQQKVTV+GY +ASKVLKKAK+TGKKAEIWPYVPY+LV+QPYIAQAYDKKAPPG
Sbjct: 61  TVEINKKQQKVTVSGYADASKVLKKAKATGKKAEIWPYVPYNLVAQPYIAQAYDKKAPPG 120

Query: 121 YVRNVEQTATTASV-TKYEDP-YINMFSDDNPNACSIM 152
           YVR V+   TT ++   Y+DP Y ++FSDDNPNACSIM
Sbjct: 121 YVRKVDPNVTTGTMAVYYDDPSYTSLFSDDNPNACSIM 158

[Close]

BLAST of Lsi01G007390 vs. TAIR10
Match: AT4G08570.1 (AT4G08570.1 Heavy metal transport/detoxification superfamily protein )

HSP 1 Score: 182.2 bits (461), Expect = 2.4e-46
Identity = 91/152 (59.87%), Postives = 119/152 (78.29%), Query Frame = 1


Query: 1   MGVGGTLDYLSDLVGSSHKHKKKKQLQTVELKV-RMDCDGCELKVKNALSSLSGVKSVEI 60
           MGV GT++Y+SDL+    K KKKKQ+QTV L+V R+DC+GCE K+K+ LS + GVKSV++
Sbjct: 1   MGVEGTMEYISDLL-KKRKRKKKKQMQTVALRVARIDCEGCERKIKHVLSGVKGVKSVDV 60

Query: 61  NRKQQKVTVTGYVEASKVLKKAKSTGKKAEIWPYVPYSLVSQPYIAQAYDKKAPPGYVRN 120
           + K QKVTVTGY++  KVL+ AKST KK E+WPYVPY++V+ PYI+QAYDKKAPP  VR 
Sbjct: 61  DVKLQKVTVTGYIDPKKVLEAAKSTKKKVELWPYVPYTMVANPYISQAYDKKAPPNMVRK 120

Query: 121 VEQTATTASVTKYEDPYINMFSDDNPNACSIM 152
           V  TA + + T  +D Y  MFSD+NPN+C+IM
Sbjct: 121 VPDTA-SVNETTVDDSYTIMFSDENPNSCAIM 150

[Close]

BLAST of Lsi01G007390 vs. TAIR10
Match: AT1G71050.1 (AT1G71050.1 Heavy metal transport/detoxification superfamily protein )

HSP 1 Score: 179.5 bits (454), Expect = 1.6e-45
Identity = 85/144 (59.03%), Postives = 110/144 (76.39%), Query Frame = 1


Query: 8   DYLSDLVGSSHKHKKKKQLQTVELKVRMDCDGCELKVKNALSSLSGVKSVEINRKQQKVT 67
           +Y+SD    + K +K+K +QTV +KV+MDCDGCE +VKNA+SS+ GVKSVE+NRK  KVT
Sbjct: 9   EYISDYFRVTRKRRKRKVMQTVNIKVKMDCDGCERRVKNAVSSMKGVKSVEVNRKIHKVT 68

Query: 68  VTGYVEASKVLKKAKSTGKKAEIWPYVPYSLVSQPYIAQAYDKKAPPGYVRNVEQTATTA 127
           V+GYVE  KVLK+ + TGKKAEIWPYVPY++V+ PY    YDKKAP GYVR  EQ+    
Sbjct: 69  VSGYVEPKKVLKRIERTGKKAEIWPYVPYNMVAYPYAVGTYDKKAPAGYVRKSEQSQLQL 128

Query: 128 SVTKYEDPYINMFSDDNPNACSIM 152
                E+ YI++FSD+NPNAC++M
Sbjct: 129 LPGAPENHYISLFSDENPNACTVM 152

[Close]

BLAST of Lsi01G007390 vs. TAIR10
Match: AT1G22990.1 (AT1G22990.1 Heavy metal transport/detoxification superfamily protein )

HSP 1 Score: 172.9 bits (437), Expect = 1.5e-43
Identity = 86/153 (56.21%), Postives = 113/153 (73.86%), Query Frame = 1


Query: 5   GTLDYLSDLVG-----SSHKHKKKKQLQTVELKVRMDCDGCELKVKNALSSLSGVKSVEI 64
           G L+YLS+        S  K KK+K +QTV +KV++DCDGCE K+KNA+SS+ G KSVE+
Sbjct: 2   GALNYLSEYFSNHFYVSIRKRKKRKVMQTVNIKVKIDCDGCERKIKNAVSSIKGAKSVEV 61

Query: 65  NRKQQKVTVTGYVEASKVLKKAKSTG-KKAEIWPYVPYSLVSQPYIAQAYDKKAPPGYVR 124
           NRK  KVTV+GYV+  KVLK  +STG KKAE+WPYVPY++V+ PY A AYDK+APPG+VR
Sbjct: 62  NRKMHKVTVSGYVDPKKVLKTVQSTGKKKAELWPYVPYTMVAYPYAAGAYDKRAPPGFVR 121

Query: 125 NVEQTATTASVTKYEDPYINMFSDDNPNACSIM 152
             EQ       T  +D  +++FSD+NPNAC++M
Sbjct: 122 KSEQAQAQPGST--DDKLMSLFSDENPNACTVM 152

[Close]

BLAST of Lsi01G007390 vs. TAIR10
Match: AT5G17450.1 (AT5G17450.1 Heavy metal transport/detoxification superfamily protein )

HSP 1 Score: 155.6 bits (392), Expect = 2.4e-38
Identity = 76/150 (50.67%), Postives = 108/150 (72.00%), Query Frame = 1


Query: 5   GTLDYLSDLVGSSHKHKK--KKQLQTVELKVRMDCDGCELKVKNALSSLSGVKSVEINRK 64
           G  DY+S     ++ + K  +K LQTV++KV+MDCDGCE +V+N +  + GVKSVE+NRK
Sbjct: 2   GAFDYISSFCSYTYANAKTKRKPLQTVDIKVKMDCDGCERRVRNVVRRMKGVKSVEVNRK 61

Query: 65  QQKVTVTGYVEASKVLKKAKSTGKKAEIWPYVPYSLVSQPYIAQAYDKKAPPGYVRNVEQ 124
           Q ++TV G+V+ +KVLK+ KSTGKKAE WPY+P  +V  P+    YDK+AP G++RN  Q
Sbjct: 62  QSRITVNGHVDPNKVLKRVKSTGKKAEFWPYIPQHMVYYPFAPGMYDKRAPAGHIRNPTQ 121

Query: 125 TATTASVTKYEDPYINMFSDDNPN-ACSIM 152
           +  TA+    E+ Y+++FSDDN + ACSIM
Sbjct: 122 SFPTANAP--EENYVSLFSDDNVHAACSIM 149

[Close]

BLAST of Lsi01G007390 vs. NCBI nr
Match: gi|659080140|ref|XP_008440632.1| (PREDICTED: heavy metal-associated isoprenylated plant protein 26 [Cucumis melo])

HSP 1 Score: 297.7 bits (761), Expect = 1.1e-77
Identity = 148/151 (98.01%), Postives = 151/151 (100.00%), Query Frame = 1


Query: 1   MGVGGTLDYLSDLVGSSHKHKKKKQLQTVELKVRMDCDGCELKVKNALSSLSGVKSVEIN 60
           MGVGGTL+YLSDLVG++HKHKKKKQLQTVELKVRMDCDGCELKVKNALSSLSGVKSVEIN
Sbjct: 1   MGVGGTLEYLSDLVGNTHKHKKKKQLQTVELKVRMDCDGCELKVKNALSSLSGVKSVEIN 60

Query: 61  RKQQKVTVTGYVEASKVLKKAKSTGKKAEIWPYVPYSLVSQPYIAQAYDKKAPPGYVRNV 120
           RKQQKVTVTGYVEASKVLKKAKSTGKKAEIWPYVPYSLVSQPYIAQAYDKKAPPGYVRNV
Sbjct: 61  RKQQKVTVTGYVEASKVLKKAKSTGKKAEIWPYVPYSLVSQPYIAQAYDKKAPPGYVRNV 120

Query: 121 EQTATTASVTKYEDPYINMFSDDNPNACSIM 152
           EQTATTASVTKYEDPYINMFSDDNPNACSIM
Sbjct: 121 EQTATTASVTKYEDPYINMFSDDNPNACSIM 151

[Close]

BLAST of Lsi01G007390 vs. NCBI nr
Match: gi|449451469|ref|XP_004143484.1| (PREDICTED: heavy metal-associated isoprenylated plant protein 26 [Cucumis sativus])

HSP 1 Score: 297.0 bits (759), Expect = 1.9e-77
Identity = 146/151 (96.69%), Postives = 151/151 (100.00%), Query Frame = 1


Query: 1   MGVGGTLDYLSDLVGSSHKHKKKKQLQTVELKVRMDCDGCELKVKNALSSLSGVKSVEIN 60
           MGVGGTL+YLSDLVG++HKHKKKKQLQTVELKVRMDCDGCELKVKNALSSLSGVKSVEIN
Sbjct: 1   MGVGGTLEYLSDLVGNTHKHKKKKQLQTVELKVRMDCDGCELKVKNALSSLSGVKSVEIN 60

Query: 61  RKQQKVTVTGYVEASKVLKKAKSTGKKAEIWPYVPYSLVSQPYIAQAYDKKAPPGYVRNV 120
           RKQQKVTVTGYVEASK+LKKAKSTGKKAEIWPYVPYSLVSQPYIAQAYDKKAPPGYVRNV
Sbjct: 61  RKQQKVTVTGYVEASKILKKAKSTGKKAEIWPYVPYSLVSQPYIAQAYDKKAPPGYVRNV 120

Query: 121 EQTATTASVTKYEDPYINMFSDDNPNACSIM 152
           EQTATTASVTKYEDPYINMFSDDNPNACS+M
Sbjct: 121 EQTATTASVTKYEDPYINMFSDDNPNACSVM 151

[Close]

BLAST of Lsi01G007390 vs. NCBI nr
Match: gi|1009146444|ref|XP_015890885.1| (PREDICTED: heavy metal-associated isoprenylated plant protein 26 [Ziziphus jujuba])

HSP 1 Score: 270.8 bits (691), Expect = 1.5e-69
Identity = 133/151 (88.08%), Postives = 144/151 (95.36%), Query Frame = 1


Query: 1   MGVGGTLDYLSDLVGSSHKHKKKKQLQTVELKVRMDCDGCELKVKNALSSLSGVKSVEIN 60
           MGVGGTL+YLSDL+GS+ K+KKKKQLQTVELKVRMDCDGCELKVK ALSSLSGVKSV+IN
Sbjct: 1   MGVGGTLEYLSDLMGSTQKYKKKKQLQTVELKVRMDCDGCELKVKKALSSLSGVKSVDIN 60

Query: 61  RKQQKVTVTGYVEASKVLKKAKSTGKKAEIWPYVPYSLVSQPYIAQAYDKKAPPGYVRNV 120
           RKQQKVTVTGYVEASKVLKKAKSTGKKAE+WPYVPY+LV+QPY AQAYDKKAP GYVRNV
Sbjct: 61  RKQQKVTVTGYVEASKVLKKAKSTGKKAELWPYVPYNLVAQPYTAQAYDKKAPAGYVRNV 120

Query: 121 EQTATTASVTKYEDPYINMFSDDNPNACSIM 152
           E TATT +VT+YEDPY +MFSDDNPNACSIM
Sbjct: 121 EYTATTGTVTRYEDPYTSMFSDDNPNACSIM 151

[Close]

BLAST of Lsi01G007390 vs. NCBI nr
Match: gi|255576174|ref|XP_002528981.1| (PREDICTED: heavy metal-associated isoprenylated plant protein 26 [Ricinus communis])

HSP 1 Score: 266.5 bits (680), Expect = 2.8e-68
Identity = 133/153 (86.93%), Postives = 146/153 (95.42%), Query Frame = 1


Query: 1   MGVGGTLDYLSDLVGSS-HKHKK-KKQLQTVELKVRMDCDGCELKVKNALSSLSGVKSVE 60
           MGVGGTL+YLSDL+GSS HK+KK KKQLQTVELK+RMDCDGCELKVKNALSS+SGVK VE
Sbjct: 1   MGVGGTLEYLSDLMGSSGHKYKKKKKQLQTVELKIRMDCDGCELKVKNALSSMSGVKKVE 60

Query: 61  INRKQQKVTVTGYVEASKVLKKAKSTGKKAEIWPYVPYSLVSQPYIAQAYDKKAPPGYVR 120
           INRKQQKVTVTGYV+ +KVLKKAKSTGKKAEIWPYVPY+LV+QPYIAQAYDKKAPPGYVR
Sbjct: 61  INRKQQKVTVTGYVDPNKVLKKAKSTGKKAEIWPYVPYNLVAQPYIAQAYDKKAPPGYVR 120

Query: 121 NVEQTATTASVTKYEDPYINMFSDDNPNACSIM 152
           NVE TAT+ +VT+YEDPY +MFSDDNPNACSIM
Sbjct: 121 NVENTATSGTVTRYEDPYSSMFSDDNPNACSIM 153

[Close]

BLAST of Lsi01G007390 vs. NCBI nr
Match: gi|645218863|ref|XP_008232814.1| (PREDICTED: heavy metal-associated isoprenylated plant protein 26-like [Prunus mume])

HSP 1 Score: 266.2 bits (679), Expect = 3.6e-68
Identity = 134/155 (86.45%), Postives = 145/155 (93.55%), Query Frame = 1


Query: 1   MGVGGTLDYLSDLV-GSSHKHKKKKQLQTVELKVRMDCDGCELKVKNALSSLSGVKSVEI 60
           MGVGGTL+YLSDLV  SSHKHKKKKQLQTVELKVRMDCDGCELKVK ALSSLSGVKSVEI
Sbjct: 1   MGVGGTLEYLSDLVHNSSHKHKKKKQLQTVELKVRMDCDGCELKVKKALSSLSGVKSVEI 60

Query: 61  NRKQQKVTVTGYVEASKVLKKAKSTGKKAEIWPYVPYSLVSQPYIAQAYDKKAPPGYVRN 120
           NRKQQKV+VTGYVEA+KVLKKAKSTGK+AEIWPY+PY+LV+QPYIAQAYDKKAPPGYVRN
Sbjct: 61  NRKQQKVSVTGYVEANKVLKKAKSTGKRAEIWPYIPYNLVAQPYIAQAYDKKAPPGYVRN 120

Query: 121 VE---QTATTASVTKYEDPYINMFSDDNPNACSIM 152
           VE    T++T +VT+YEDPY  MFSDDNPNACSIM
Sbjct: 121 VENMNMTSSTGTVTRYEDPYTTMFSDDNPNACSIM 155

The following BLAST results are available for this feature:

BLAST of Lsi01G007390 vs. Swiss-Prot


Match Name	E-value	Identity	Description
HIP20_ARATH	2.8e-44	59.03	Heavy metal-associated isoprenylated plant protein 20 OS=Arabidopsis thaliana GN...	[more]
HIP22_ARATH	2.6e-42	56.21	Heavy metal-associated isoprenylated plant protein 22 OS=Arabidopsis thaliana GN...	[more]
HIP21_ARATH	4.3e-37	50.67	Heavy metal-associated isoprenylated plant protein 21 OS=Arabidopsis thaliana GN...	[more]
HIP26_ARATH	3.6e-36	50.66	Heavy metal-associated isoprenylated plant protein 26 OS=Arabidopsis thaliana GN...	[more]
HIP27_ARATH	8.9e-35	53.68	Heavy metal-associated isoprenylated plant protein 27 OS=Arabidopsis thaliana GN...	[more]

BLAST of Lsi01G007390 vs. TrEMBL


Match Name	E-value	Identity	Description
A0A0A0KIY2_CUCSA	1.3e-77	96.69	Uncharacterized protein OS=Cucumis sativus GN=Csa_6G500660 PE=4 SV=1	[more]
B9SSL2_RICCO	1.9e-68	86.93	Metal ion binding protein, putative OS=Ricinus communis GN=RCOM_0985130 PE=4 SV=...	[more]
M5W0M5_PRUPE	3.6e-67	85.16	Uncharacterized protein OS=Prunus persica GN=PRUPE_ppa012771mg PE=4 SV=1	[more]
V4S1Z4_9ROSI	1.1e-66	86.93	Uncharacterized protein OS=Citrus clementina GN=CICLE_v10026695mg PE=4 SV=1	[more]
B9H568_POPTR	1.1e-66	84.97	Heavy-metal-associated domain-containing family protein OS=Populus trichocarpa G...	[more]

BLAST of Lsi01G007390 vs. TAIR10


Match Name	E-value	Identity	Description
AT4G39700.1	2.0e-61	73.42	Heavy metal transport/detoxification superfamily protein	[more]
AT4G08570.1	2.4e-46	59.87	Heavy metal transport/detoxification superfamily protein	[more]
AT1G71050.1	1.6e-45	59.03	Heavy metal transport/detoxification superfamily protein	[more]
AT1G22990.1	1.5e-43	56.21	Heavy metal transport/detoxification superfamily protein	[more]
AT5G17450.1	2.4e-38	50.67	Heavy metal transport/detoxification superfamily protein	[more]

BLAST of Lsi01G007390 vs. NCBI nr


Match Name	E-value	Identity	Description
gi\|659080140\|ref\|XP_008440632.1\|	1.1e-77	98.01	PREDICTED: heavy metal-associated isoprenylated plant protein 26 [Cucumis melo]	[more]
gi\|449451469\|ref\|XP_004143484.1\|	1.9e-77	96.69	PREDICTED: heavy metal-associated isoprenylated plant protein 26 [Cucumis sativu...	[more]
gi\|1009146444\|ref\|XP_015890885.1\|	1.5e-69	88.08	PREDICTED: heavy metal-associated isoprenylated plant protein 26 [Ziziphus jujub...	[more]
gi\|255576174\|ref\|XP_002528981.1\|	2.8e-68	86.93	PREDICTED: heavy metal-associated isoprenylated plant protein 26 [Ricinus commun...	[more]
gi\|645218863\|ref\|XP_008232814.1\|	3.6e-68	86.45	PREDICTED: heavy metal-associated isoprenylated plant protein 26-like [Prunus mu...	[more]

Annotated Terms

The following terms have been associated with this gene:

Vocabulary: **Molecular Function**
Term	Definition
GO:0046872	metal ion binding

Vocabulary: **Biological Process**
Term	Definition
GO:0030001	metal ion transport

Vocabulary: **INTERPRO**
Term	Definition
IPR006121	HMA_dom

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0046916	cellular transition metal ion homeostasis
biological_process	GO:0030001	metal ion transport
biological_process	GO:0006351	transcription, DNA-templated
cellular_component	GO:0005737	cytoplasm
cellular_component	GO:0005575	cellular_component
molecular_function	GO:0046914	transition metal ion binding
molecular_function	GO:0046872	metal ion binding

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Lsi01G007390.1	Lsi01G007390.1	mRNA

InterPro

Analysis Name: InterPro Annotations of Lagenaria siceraria
Date Performed: 2017-09-18


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR006121	Heavy metal-associated domain, HMA	PFAM	PF00403	HMA	coord: 32..87 score: 6.1
IPR006121	Heavy metal-associated domain, HMA	PROFILE	PS50846	HMA_2	coord: 35..90 score: 11
IPR006121	Heavy metal-associated domain, HMA	unknown	SSF55008	HMA, heavy metal-associated domain	coord: 26..92 score: 4.45
None	No IPR available	GENE3D	G3DSA:3.30.70.100		coord: 25..92 score: 1.5
None	No IPR available	PANTHER	PTHR22814	COPPER TRANSPORT PROTEIN ATOX1-RELATED	coord: 14..150 score: 1.9
None	No IPR available	PANTHER	PTHR22814:SF126	SUBFAMILY NOT NAMED	coord: 14..150 score: 1.9

Synteny

The following gene(s) are orthologous to this gene:


Gene	Orthologue	Organism	Block
Lsi01G007390	Csa6G500660	Cucumber (Chinese Long) v2	culsiB434
Lsi01G007390	CSPI06G30470	Wild cucumber (PI 183967)	cpilsiB439
Lsi01G007390	Cucsa.104620	Cucumber (Gy14) v1	cgylsiB125
Lsi01G007390	CmaCh03G007950	Cucurbita maxima (Rimu)	cmalsiB597
Lsi01G007390	CmaCh04G007960	Cucurbita maxima (Rimu)	cmalsiB639
Lsi01G007390	CmaCh07G005250	Cucurbita maxima (Rimu)	cmalsiB786
Lsi01G007390	CmoCh03G008290	Cucurbita moschata (Rifu)	cmolsiB582
Lsi01G007390	CmoCh04G008550	Cucurbita moschata (Rifu)	cmolsiB628
Lsi01G007390	CmoCh07G005220	Cucurbita moschata (Rifu)	cmolsiB774
Lsi01G007390	Cla014166	Watermelon (97103) v1	lsiwmB144
Lsi01G007390	MELO3C007779	Melon (DHL92) v3.5.1	lsimeB156
Lsi01G007390	ClCG01G019000	Watermelon (Charleston Gray)	lsiwcgB127
Lsi01G007390	MELO3C007779.2	Melon (DHL92) v3.6.1	lsimedB163
Lsi01G007390	Cla97C01G018480	Watermelon (97103) v2	lsiwmbB115
Lsi01G007390	Bhi03G000940	Wax gourd	lsiwgoB181
Lsi01G007390	Cp4.1LG10g01120	Cucurbita pepo (Zucchini)	cpelsiB054
Lsi01G007390	Cp4.1LG01g09640	Cucurbita pepo (Zucchini)	cpelsiB326
Lsi01G007390	Cp4.1LG19g09610	Cucurbita pepo (Zucchini)	cpelsiB396
Lsi01G007390	CsGy6G029970	Cucumber (Gy14) v2	cgyblsiB398
Lsi01G007390	Carg14196	Silver-seed gourd	carlsiB855
Lsi01G007390	Carg16014	Silver-seed gourd	carlsiB693
Lsi01G007390	Carg00680	Silver-seed gourd	carlsiB364
Lsi01G007390	CsaV3_6G045690	Cucumber (Chinese Long) v3	cuclsiB462

The following gene(s) are paralogous to this gene:

None

The following block(s) are covering this gene:

None