Cucsa.366650 (gene) Cucumber (Gy14) v1

Overview
NameCucsa.366650
Typegene
OrganismCucumis sativus (Cucumber (Gy14) v1)
DescriptionProteinase inhibitor I13, potato inhibitor I
Locationscaffold03625 : 203918 .. 204114 (-)
Genome Viewer
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: CDS
Hold the cursor over a type above to highlight its positions in the sequence below.
GAGGGAATTATAGATGGCCTAACCTAGTAGGTAAGAGATGGCAATATGCTAAAAGGAAAATTGAAGAAGAACTTCCAAGAGTGGGTGTCGCCGTTATGAGAAGAGGGGCGATCAGAATCGAAGATTTCTGTTGCAACCGTGTCATTGTTTACGTCGATGACAGTGGCATTGTTGTTGAAGTCCCAGTCATTGGTTGA

mRNA sequence

GAGGGAATTATAGATGGCCTAACCTAGTAGGTAAGAGATGGCAATATGCTAAAAGGAAAATTGAAGAAGAACTTCCAAGAGTGGGTGTCGCCGTTATGAGAAGAGGGGCGATCAGAATCGAAGATTTCTGTTGCAACCGTGTCATTGTTTACGTCGATGACAGTGGCATTGTTGTTGAAGTCCCAGTCATTGGTTGA

Coding sequence (CDS)

GAGGGAATTATAGATGGCCTAACCTAGTAGGTAAGAGATGGCAATATGCTAAAAGGAAAATTGAAGAAGAACTTCCAAGAGTGGGTGTCGCCGTTATGAGAAGAGGGGCGATCAGAATCGAAGATTTCTGTTGCAACCGTGTCATTGTTTACGTCGATGACAGTGGCATTGTTGTTGAAGTCCCAGTCATTGGTTGA

Protein sequence

GNYRWPNLVGKRWQYAKRKIEEELPRVGVAVMRRGAIRIEDFCCNRVIVYVDDSGIVVEVPVIG*
Homology
[Close]
BLAST of Cucsa.366650 vs. Swiss-Prot
Match: BGIA_MOMCH (Glu S.griseus protease inhibitor OS=Momordica charantia PE=1 SV=1)

HSP 1 Score: 54.3 bits (129), Expect = 5.8e-07
Identity = 29/64 (45.31%), Postives = 35/64 (54.69%), Query Frame = 1 

Query: 1  GNYRWPNLVGKRWQYAKRKIEEELPRVGVAVMRRGAIRIEDFCCNRVIVYVDDSGIVVEV 60
          G   WP LVG     AK  IE E PRV   ++R G+    DF C+RV V+V + GIV   
Sbjct: 5  GKRSWPQLVGSTGAAAKAVIERENPRVRAVIVRVGSPVTADFRCDRVRVWVTERGIVARP 64

Query: 61 PVIG 65
          P IG
Sbjct: 65 PAIG 68

[Close]
BLAST of Cucsa.366650 vs. Swiss-Prot
Match: ICI_LINUS (Proteinase inhibitor OS=Linum usitatissimum PE=1 SV=1)

HSP 1 Score: 51.6 bits (122), Expect = 3.7e-06
Identity = 26/63 (41.27%), Postives = 34/63 (53.97%), Query Frame = 1 

Query: 1  GNYRWPNLVGKRWQYAKRKIEEELPRVGVAVMRRGAIRIEDFCCNRVIVYVDDSGIVVEV 60
          G   WP LVGK    A   +E E   V   V++ G+   +DF C+RV V V+D G+V  V
Sbjct: 6  GKNAWPELVGKSGNMAAATVERENRNVHAIVLKEGSAMTKDFRCDRVWVIVNDHGVVTSV 65

Query: 61 PVI 64
          P I
Sbjct: 66 PHI 68

[Close]
BLAST of Cucsa.366650 vs. Swiss-Prot
Match: ITH5_CUCMA (Inhibitor of trypsin and hageman factor OS=Cucurbita maxima PE=1 SV=1)

HSP 1 Score: 51.6 bits (122), Expect = 3.7e-06
Identity = 26/64 (40.62%), Postives = 35/64 (54.69%), Query Frame = 1 

Query: 1  GNYRWPNLVGKRWQYAKRKIEEELPRVGVAVMRRGAIRIEDFCCNRVIVYVDDSGIVVEV 60
          G   WP+LVG     AK  IE + P V   ++  G    +DF CNRV ++V+  G+VV  
Sbjct: 5  GKSSWPHLVGVGGSVAKAIIERQNPNVKAVILEEGTPVTKDFRCNRVRIWVNKRGLVVSP 64

Query: 61 PVIG 65
          P IG
Sbjct: 65 PRIG 68

[Close]
BLAST of Cucsa.366650 vs. TrEMBL
Match: A0A0A0LJT7_CUCSA (Uncharacterized protein OS=Cucumis sativus GN=Csa_2G287050 PE=4 SV=1)

HSP 1 Score: 136.3 bits (342), Expect = 1.3e-29
Identity = 64/64 (100.00%), Postives = 64/64 (100.00%), Query Frame = 1 

Query: 1  GNYRWPNLVGKRWQYAKRKIEEELPRVGVAVMRRGAIRIEDFCCNRVIVYVDDSGIVVEV 60
          GNYRWPNLVGKRWQYAKRKIEEELPRVGVAVMRRGAIRIEDFCCNRVIVYVDDSGIVVEV
Sbjct: 9  GNYRWPNLVGKRWQYAKRKIEEELPRVGVAVMRRGAIRIEDFCCNRVIVYVDDSGIVVEV 68

Query: 61 PVIG 65
          PVIG
Sbjct: 69 PVIG 72

[Close]
BLAST of Cucsa.366650 vs. TrEMBL
Match: A0MF11_ARATH (Putative uncharacterized protein (Fragment) OS=Arabidopsis thaliana PE=2 SV=1)

HSP 1 Score: 73.6 bits (179), Expect = 1.0e-10
Identity = 35/62 (56.45%), Postives = 41/62 (66.13%), Query Frame = 1 

Query: 3  YRWPNLVGKRWQYAKRKIEEELPRVGVAVMRRGAIRIEDFCCNRVIVYVDDSGIVVEVPV 62
          YRWP LVGK  Q AK  IE E P V   V+R G  RIE+FCCNRV VY+  +G V + P+
Sbjct: 24 YRWPELVGKNGQLAKMTIERENPNVLAIVLRYGEKRIENFCCNRVFVYLGSNGQVADAPM 83

Query: 63 IG 65
          IG
Sbjct: 84 IG 85

[Close]
BLAST of Cucsa.366650 vs. TrEMBL
Match: Q9STF8_ARATH (PR-6 proteinase inhibitor family protein OS=Arabidopsis thaliana GN=T6H20.110 PE=2 SV=1)

HSP 1 Score: 73.6 bits (179), Expect = 1.0e-10
Identity = 35/62 (56.45%), Postives = 41/62 (66.13%), Query Frame = 1 

Query: 3  YRWPNLVGKRWQYAKRKIEEELPRVGVAVMRRGAIRIEDFCCNRVIVYVDDSGIVVEVPV 62
          YRWP LVGK  Q AK  IE E P V   V+R G  RIE+FCCNRV VY+  +G V + P+
Sbjct: 24 YRWPELVGKNGQLAKMTIERENPNVLAIVLRYGEKRIENFCCNRVFVYLGSNGQVADAPM 83

Query: 63 IG 65
          IG
Sbjct: 84 IG 85

[Close]
BLAST of Cucsa.366650 vs. TrEMBL
Match: V4NI49_EUTSA (Uncharacterized protein OS=Eutrema salsugineum GN=EUTSA_v10011055mg PE=4 SV=1)

HSP 1 Score: 68.9 bits (167), Expect = 2.5e-09
Identity = 33/64 (51.56%), Postives = 39/64 (60.94%), Query Frame = 1 

Query: 1  GNYRWPNLVGKRWQYAKRKIEEELPRVGVAVMRRGAIRIEDFCCNRVIVYVDDSGIVVEV 60
          G + WP LVGK  + AK  IE E P V   +M  G +RIEDFCCNRV + V   G VV +
Sbjct: 25 GKFEWPELVGKSGEIAKMTIERENPNVLGFIMPYGTLRIEDFCCNRVFIVVGSKGHVVMI 84

Query: 61 PVIG 65
          P IG
Sbjct: 85 PKIG 88

[Close]
BLAST of Cucsa.366650 vs. TrEMBL
Match: R0GPN4_9BRAS (Uncharacterized protein OS=Capsella rubella GN=CARUB_v10027484mg PE=4 SV=1)

HSP 1 Score: 65.5 bits (158), Expect = 2.8e-08
Identity = 30/64 (46.88%), Postives = 41/64 (64.06%), Query Frame = 1 

Query: 1  GNYRWPNLVGKRWQYAKRKIEEELPRVGVAVMRRGAIRIEDFCCNRVIVYVDDSGIVVEV 60
          G   WP L+G R +YAK  IE E P++G  ++  G    E F C+RV V+V+D G+VV+V
Sbjct: 9  GKSSWPELIGTRGEYAKEVIERENPKMGAVIILDGTAVPEIFICSRVYVWVNDLGVVVQV 68

Query: 61 PVIG 65
          P IG
Sbjct: 69 PTIG 72

[Close]
BLAST of Cucsa.366650 vs. TAIR10
Match: AT3G46860.1 (AT3G46860.1 Serine protease inhibitor, potato inhibitor I-type family protein)

HSP 1 Score: 73.6 bits (179), Expect = 5.2e-14
Identity = 35/62 (56.45%), Postives = 41/62 (66.13%), Query Frame = 1 

Query: 3  YRWPNLVGKRWQYAKRKIEEELPRVGVAVMRRGAIRIEDFCCNRVIVYVDDSGIVVEVPV 62
          YRWP LVGK  Q AK  IE E P V   V+R G  RIE+FCCNRV VY+  +G V + P+
Sbjct: 24 YRWPELVGKNGQLAKMTIERENPNVLAIVLRYGEKRIENFCCNRVFVYLGSNGQVADAPM 83

Query: 63 IG 65
          IG
Sbjct: 84 IG 85

[Close]
BLAST of Cucsa.366650 vs. TAIR10
Match: AT5G43580.1 (AT5G43580.1 Serine protease inhibitor, potato inhibitor I-type family protein)

HSP 1 Score: 59.7 bits (143), Expect = 7.8e-10
Identity = 27/64 (42.19%), Postives = 41/64 (64.06%), Query Frame = 1 

Query: 1  GNYRWPNLVGKRWQYAKRKIEEELPRVGVAVMRRGAIRIEDFCCNRVIVYVDDSGIVVEV 60
          G   WP L+G + + AK  IE E P++   ++  G +  E F C+RV V+V+D GIVV++
Sbjct: 36 GKSSWPELLGAKGEDAKEVIERENPKMKAVIILDGTVVPEIFICSRVYVWVNDCGIVVQI 95

Query: 61 PVIG 65
          P+IG
Sbjct: 96 PIIG 99

[Close]
BLAST of Cucsa.366650 vs. TAIR10
Match: AT2G38870.1 (AT2G38870.1 Serine protease inhibitor, potato inhibitor I-type family protein)

HSP 1 Score: 49.7 bits (117), Expect = 8.0e-07
Identity = 27/60 (45.00%), Postives = 33/60 (55.00%), Query Frame = 1 

Query: 5  WPNLVGKRWQYAKRKIEEELPRVGVAVMRRGAIRIEDFCCNRVIVYVDDSGIVVEVPVIG 64
          WP L G    YA   IE E P V  AV+  G+    DF C+RV V+VD + IVV+ P  G
Sbjct: 11 WPELTGTNGDYAAVVIERENPTVNAAVILDGSPVTADFRCDRVRVFVDGNRIVVKTPKSG 70

[Close]
BLAST of Cucsa.366650 vs. NCBI nr
Match: gi|700206904|gb|KGN62023.1| (hypothetical protein Csa_2G287050 [Cucumis sativus])

HSP 1 Score: 136.3 bits (342), Expect = 1.8e-29
Identity = 64/64 (100.00%), Postives = 64/64 (100.00%), Query Frame = 1 

Query: 1  GNYRWPNLVGKRWQYAKRKIEEELPRVGVAVMRRGAIRIEDFCCNRVIVYVDDSGIVVEV 60
          GNYRWPNLVGKRWQYAKRKIEEELPRVGVAVMRRGAIRIEDFCCNRVIVYVDDSGIVVEV
Sbjct: 9  GNYRWPNLVGKRWQYAKRKIEEELPRVGVAVMRRGAIRIEDFCCNRVIVYVDDSGIVVEV 68

Query: 61 PVIG 65
          PVIG
Sbjct: 69 PVIG 72

[Close]
BLAST of Cucsa.366650 vs. NCBI nr
Match: gi|15232657|ref|NP_190270.1| (PR-6 proteinase inhibitor family protein [Arabidopsis thaliana])

HSP 1 Score: 73.6 bits (179), Expect = 1.5e-10
Identity = 35/62 (56.45%), Postives = 41/62 (66.13%), Query Frame = 1 

Query: 3  YRWPNLVGKRWQYAKRKIEEELPRVGVAVMRRGAIRIEDFCCNRVIVYVDDSGIVVEVPV 62
          YRWP LVGK  Q AK  IE E P V   V+R G  RIE+FCCNRV VY+  +G V + P+
Sbjct: 24 YRWPELVGKNGQLAKMTIERENPNVLAIVLRYGEKRIENFCCNRVFVYLGSNGQVADAPM 83

Query: 63 IG 65
          IG
Sbjct: 84 IG 85

[Close]
BLAST of Cucsa.366650 vs. NCBI nr
Match: gi|116831272|gb|ABK28590.1| (unknown [Arabidopsis thaliana])

HSP 1 Score: 73.6 bits (179), Expect = 1.5e-10
Identity = 35/62 (56.45%), Postives = 41/62 (66.13%), Query Frame = 1 

Query: 3  YRWPNLVGKRWQYAKRKIEEELPRVGVAVMRRGAIRIEDFCCNRVIVYVDDSGIVVEVPV 62
          YRWP LVGK  Q AK  IE E P V   V+R G  RIE+FCCNRV VY+  +G V + P+
Sbjct: 24 YRWPELVGKNGQLAKMTIERENPNVLAIVLRYGEKRIENFCCNRVFVYLGSNGQVADAPM 83

Query: 63 IG 65
          IG
Sbjct: 84 IG 85

[Close]
BLAST of Cucsa.366650 vs. NCBI nr
Match: gi|567189624|ref|XP_006404448.1| (hypothetical protein EUTSA_v10011055mg [Eutrema salsugineum])

HSP 1 Score: 68.9 bits (167), Expect = 3.6e-09
Identity = 33/64 (51.56%), Postives = 39/64 (60.94%), Query Frame = 1 

Query: 1  GNYRWPNLVGKRWQYAKRKIEEELPRVGVAVMRRGAIRIEDFCCNRVIVYVDDSGIVVEV 60
          G + WP LVGK  + AK  IE E P V   +M  G +RIEDFCCNRV + V   G VV +
Sbjct: 25 GKFEWPELVGKSGEIAKMTIERENPNVLGFIMPYGTLRIEDFCCNRVFIVVGSKGHVVMI 84

Query: 61 PVIG 65
          P IG
Sbjct: 85 PKIG 88

[Close]
BLAST of Cucsa.366650 vs. NCBI nr
Match: gi|565435839|ref|XP_006281417.1| (hypothetical protein CARUB_v10027484mg [Capsella rubella])

HSP 1 Score: 65.5 bits (158), Expect = 4.0e-08
Identity = 30/64 (46.88%), Postives = 41/64 (64.06%), Query Frame = 1 

Query: 1  GNYRWPNLVGKRWQYAKRKIEEELPRVGVAVMRRGAIRIEDFCCNRVIVYVDDSGIVVEV 60
          G   WP L+G R +YAK  IE E P++G  ++  G    E F C+RV V+V+D G+VV+V
Sbjct: 9  GKSSWPELIGTRGEYAKEVIERENPKMGAVIILDGTAVPEIFICSRVYVWVNDLGVVVQV 68

Query: 61 PVIG 65
          P IG
Sbjct: 69 PTIG 72

The following BLAST results are available for this feature:
BLAST of Cucsa.366650 vs. Swiss-Prot
Match NameE-valueIdentityDescription
BGIA_MOMCH5.8e-0745.31Glu S.griseus protease inhibitor OS=Momordica charantia PE=1 SV=1[more]
ICI_LINUS3.7e-0641.27Proteinase inhibitor OS=Linum usitatissimum PE=1 SV=1[more]
ITH5_CUCMA3.7e-0640.63Inhibitor of trypsin and hageman factor OS=Cucurbita maxima PE=1 SV=1[more]
BLAST of Cucsa.366650 vs. TrEMBL
Match NameE-valueIdentityDescription
A0A0A0LJT7_CUCSA1.3e-29100.00Uncharacterized protein OS=Cucumis sativus GN=Csa_2G287050 PE=4 SV=1[more]
A0MF11_ARATH1.0e-1056.45Putative uncharacterized protein (Fragment) OS=Arabidopsis thaliana PE=2 SV=1[more]
Q9STF8_ARATH1.0e-1056.45PR-6 proteinase inhibitor family protein OS=Arabidopsis thaliana GN=T6H20.110 PE... [more]
V4NI49_EUTSA2.5e-0951.56Uncharacterized protein OS=Eutrema salsugineum GN=EUTSA_v10011055mg PE=4 SV=1[more]
R0GPN4_9BRAS2.8e-0846.88Uncharacterized protein OS=Capsella rubella GN=CARUB_v10027484mg PE=4 SV=1[more]
BLAST of Cucsa.366650 vs. TAIR10
Match NameE-valueIdentityDescription
AT3G46860.15.2e-1456.45 Serine protease inhibitor, potato inhibitor I-type family protein[more]
AT5G43580.17.8e-1042.19 Serine protease inhibitor, potato inhibitor I-type family protein[more]
AT2G38870.18.0e-0745.00 Serine protease inhibitor, potato inhibitor I-type family protein[more]
BLAST of Cucsa.366650 vs. NCBI nr
Match NameE-valueIdentityDescription
gi|700206904|gb|KGN62023.1|1.8e-29100.00hypothetical protein Csa_2G287050 [Cucumis sativus][more]
gi|15232657|ref|NP_190270.1|1.5e-1056.45PR-6 proteinase inhibitor family protein [Arabidopsis thaliana][more]
gi|116831272|gb|ABK28590.1|1.5e-1056.45unknown [Arabidopsis thaliana][more]
gi|567189624|ref|XP_006404448.1|3.6e-0951.56hypothetical protein EUTSA_v10011055mg [Eutrema salsugineum][more]
gi|565435839|ref|XP_006281417.1|4.0e-0846.88hypothetical protein CARUB_v10027484mg [Capsella rubella][more]
Annotated Terms
The following terms have been associated with this gene:
Vocabulary: INTERPRO
TermDefinition
IPR000864Prot_inh_pot1
Vocabulary: Molecular Function
TermDefinition
GO:0004867serine-type endopeptidase inhibitor activity
Vocabulary: Biological Process
TermDefinition
GO:0009611response to wounding
GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0010951 negative regulation of endopeptidase activity
biological_process GO:0009611 response to wounding
cellular_component GO:0005575 cellular_component
molecular_function GO:0004867 serine-type endopeptidase inhibitor activity
Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Cucsa.366650.1Cucsa.366650.1mRNA


InterPro
Analysis Name: InterPro Annotations of cucumber (Gy14)
Date Performed: 2017-01-17
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR000864Proteinase inhibitor I13, potato inhibitor IPRODOMPD002604coord: 4..64
score: 4.
IPR000864Proteinase inhibitor I13, potato inhibitor IPFAMPF00280potato_inhibitcoord: 4..64
score: 8.5
IPR000864Proteinase inhibitor I13, potato inhibitor IunknownSSF54654CI-2 family of serine protease inhibitorscoord: 3..64
score: 1.96
NoneNo IPR availableGENE3DG3DSA:3.30.10.10coord: 4..64
score: 8.7
NoneNo IPR availablePANTHERPTHR33091FAMILY NOT NAMEDcoord: 3..64
score: 7.1
NoneNo IPR availablePANTHERPTHR33091:SF13PR-6 PROTEINASE INHIBITOR FAMILY PROTEIN-RELATEDcoord: 3..64
score: 7.1

Synteny
The following gene(s) are orthologous to this gene:
GeneOrthologueOrganismBlock
Cucsa.366650Csa2G287050Cucumber (Chinese Long) v2cgycuB513
Cucsa.366650MELO3C020883Melon (DHL92) v3.5.1cgymeB597
Cucsa.366650ClCG02G012910Watermelon (Charleston Gray)cgywcgB629
Cucsa.366650CSPI02G14450Wild cucumber (PI 183967)cgycpiB542
Cucsa.366650MELO3C034586.2Melon (DHL92) v3.6.1cgymedB595
Cucsa.366650CsaV3_2G017000Cucumber (Chinese Long) v3cgycucB557
The following gene(s) are paralogous to this gene:

None

The following block(s) are covering this gene:
GeneOrganismBlock
Cucsa.366650Wax gourdcgywgoB741
Cucsa.366650Cucumber (Gy14) v1cgycgyB050
Cucsa.366650Cucumber (Gy14) v1cgycgyB051
Cucsa.366650Cucurbita maxima (Rimu)cgycmaB1023
Cucsa.366650Cucurbita maxima (Rimu)cgycmaB1015
Cucsa.366650Cucurbita maxima (Rimu)cgycmaB1021
Cucsa.366650Cucurbita maxima (Rimu)cgycmaB1022
Cucsa.366650Cucurbita moschata (Rifu)cgycmoB1015
Cucsa.366650Cucurbita moschata (Rifu)cgycmoB1020
Cucsa.366650Cucurbita moschata (Rifu)cgycmoB1021
Cucsa.366650Melon (DHL92) v3.5.1cgymeB596
Cucsa.366650Melon (DHL92) v3.5.1cgymeB598
Cucsa.366650Watermelon (97103) v1cgywmB667
Cucsa.366650Cucurbita pepo (Zucchini)cgycpeB0971
Cucsa.366650Cucurbita pepo (Zucchini)cgycpeB0973
Cucsa.366650Cucurbita pepo (Zucchini)cgycpeB0974
Cucsa.366650Bottle gourd (USVL1VR-Ls)cgylsiB595
Cucsa.366650Bottle gourd (USVL1VR-Ls)cgylsiB594
Cucsa.366650Melon (DHL92) v3.6.1cgymedB594
Cucsa.366650Silver-seed gourdcarcgyB0924
Cucsa.366650Silver-seed gourdcarcgyB0971
Cucsa.366650Silver-seed gourdcarcgyB1178
Cucsa.366650Cucumber (Chinese Long) v3cgycucB558
Cucsa.366650Watermelon (97103) v2cgywmbB635