Cucsa.362690 (gene) Cucumber (Gy14) v1

BLAST of Cucsa.362690 vs. Swiss-Prot
Match: PTR14_ARATH (Protein NRT1/ PTR FAMILY 7.3 OS=Arabidopsis thaliana GN=NPF7.3 PE=1 SV=2)

HSP 1 Score: 591.3 bits (1523), Expect = 1.3e-167
Identity = 303/567 (53.44%), Postives = 400/567 (70.55%), Query Frame = 1

Query: 92  RRKVGGWNCGMLLLVNQGLITLAFAGVEVNLVLFSKSVLRQTNAEAANMFSRWMGTTYCF 151
           R   G W  G+++L+NQGL TLAF GV VNLVLF   VL+Q NA+AAN  S+W GT Y F
Sbjct: 35  RSNSGQWVAGIVILLNQGLATLAFFGVGVNLVLFLTRVLQQNNADAANNVSKWTGTVYIF 94

Query: 152 SLLGAFLSDSYLGRYLTCVIFQLVFIVGLIALSLLTHISLLKPNGCGKIGQLCDPHSLTE 211
           SL+GAFLSDSY GRY TC IFQ++F++GL +LSL +++ L++P GCG     C  HS+ E
Sbjct: 95  SLVGAFLSDSYWGRYKTCAIFQVIFVIGLSSLSLSSYMFLIRPRGCGDEVTPCGSHSMME 154

Query: 212 LSIFYISIYLIALGNGAPEPALATFGVEQFDDKDPKENRAKTSFYSYFYVSINMGCLIAE 271
           +++FY SIYLIALG G  +P +AT G +QFD++ PKE  +K +F+SYFY+++N+G L + 
Sbjct: 155 ITMFYFSIYLIALGYGGYQPNIATLGADQFDEEHPKEGYSKIAFFSYFYLALNLGSLFSN 214

Query: 272 TILVYIENLGHWMVGFWICAVCGLLAYLLFLSGTPRYRQSKPCGNPISRFSQVLMASVKK 331
           TIL Y E+ G W +GFW      ++  +LFL GTPRYR  KP GNP+SRF QVL+A+ KK
Sbjct: 215 TILGYFEDEGMWALGFWASTGSAIIGLILFLVGTPRYRYFKPTGNPLSRFCQVLVAATKK 274

Query: 332 MNLPMPSNG-EGLYEG--LGKKGT----RRMLHTEGFKFLDRAAILTAEEANLICNHGQT 391
            ++  P  G E +Y+G   GK  +    RR++HT+ FKFLD+AA +TA +  L      +
Sbjct: 275 SSVEAPLRGREEMYDGDSEGKNASVNTGRRIVHTDEFKFLDKAAYITARD--LDDKKQDS 334

Query: 392 PNPWKICPVTQVEEVKCILRLLPVWLCTIFSSVVFIQMISLFVEQGAAMDTLVVSNFHIP 451
            NPW++CPVTQVEEVKCILRL+P+WLCTI  SVVF QM SLFVEQGAAM+T  VS+F IP
Sbjct: 335 VNPWRLCPVTQVEEVKCILRLMPIWLCTIIYSVVFTQMASLFVEQGAAMNT-SVSDFKIP 394

Query: 452 PASMTAFDIVSTTMFIMLYDRLLVPLYVRIAKRKPKAPNELQRIGIGLAIAIVALVIAGF 511
           PASM++FDI+S  +FI LY R+L P+  R  K   K   EL R+GIGL IA++A++ AG 
Sbjct: 395 PASMSSFDILSVALFIFLYRRVLEPVANRFKKNGSKGITELHRMGIGLVIAVIAMIAAGI 454

Query: 512 VEQKRLKHAGVSGKEI---SSFSIFWQTPQYVLVGVAEAFVYVAQMDFFTSQIPEGLKSL 571
           VE  RLK+A  S       SS SIFWQ PQY L+G +E F+YV Q++FF +Q P+GLKS 
Sbjct: 455 VECYRLKYADKSCTHCDGSSSLSIFWQAPQYSLIGASEVFMYVGQLEFFNAQTPDGLKSF 514

Query: 572 GMGLSMSSTAMGSYVASLILTTVMAITKTNGNPGWVPPNLNDGHLDRFFFLSAALTAFNL 631
           G  L M S +MG++V+SL++T V+ I+  +  PGW+P NLN GHLDRF+FL AALT+ +L
Sbjct: 515 GSALCMMSMSMGNFVSSLLVTMVVKISTEDHMPGWIPRNLNKGHLDRFYFLLAALTSIDL 574

Query: 632 ALYIVCAKQYKGSIIEKQDGDQEEEPD 649
            +YI CAK YK   +E +D  Q+   D
Sbjct: 575 VVYIACAKWYKPIQLEGKDEMQDMSDD 598

BLAST of Cucsa.362690 vs. Swiss-Prot
Match: PTR47_ARATH (Protein NRT1/ PTR FAMILY 7.2 OS=Arabidopsis thaliana GN=NPF7.2 PE=2 SV=2)

HSP 1 Score: 553.9 bits (1426), Expect = 2.3e-156
Identity = 285/549 (51.91%), Postives = 373/549 (67.94%), Query Frame = 1

Query: 90  ANRRKVGGWNCGMLLLVNQGLITLAFAGVEVNLVLFSKSVLRQTNAEAANMFSRWMGTTY 149
           A R   G W   +L+LVNQGL TLAF GV VNLVLF   V+ Q NAEAAN  S+W GT Y
Sbjct: 24  AIRANTGKWLTAILILVNQGLATLAFFGVGVNLVLFLTRVMGQDNAEAANNVSKWTGTVY 83

Query: 150 CFSLLGAFLSDSYLGRYLTCVIFQLVFIVGLIALSLLTHISLLKPNGCGKIGQLCDPHSL 209
            FSLLGAFLSDSY GRY TC IFQ  F+ GL+ LSL T   LL+P+GCG     C PHS 
Sbjct: 84  IFSLLGAFLSDSYWGRYKTCAIFQASFVAGLMMLSLSTGALLLEPSGCGVEDSPCKPHST 143

Query: 210 TELSIFYISIYLIALGNGAPEPALATFGVEQFDDKDPKENRAKTSFYSYFYVSINMGCLI 269
            +  +FY+S+YLIALG G  +P +ATFG +QFD +D  E  +K +F+SYFY+++N+G L 
Sbjct: 144 FKTVLFYLSVYLIALGYGGYQPNIATFGADQFDAEDSVEGHSKIAFFSYFYLALNLGSLF 203

Query: 270 AETILVYIENLGHWMVGFWICAVCGLLAYLLFLSGTPRYRQSKPCGNPISRFSQVLMASV 329
           + T+L Y E+ G W +GFW  A       +LFL GTP+YR   P  +P SRF QVL+A+ 
Sbjct: 204 SNTVLGYFEDQGEWPLGFWASAGSAFAGLVLFLIGTPKYRHFTPRESPWSRFCQVLVAAT 263

Query: 330 KKMNLPMPSNGEGLYEGLGK-KGTRRMLHTEGFKFLDRAAILTAEEANLICNHGQTPNPW 389
           +K  + +      LY+   +  G +++LHT+GF+FLDRAAI+T ++       G   +PW
Sbjct: 264 RKAKIDVHHEELNLYDSETQYTGDKKILHTKGFRFLDRAAIVTPDDEAEKVESGSKYDPW 323

Query: 390 KICPVTQVEEVKCILRLLPVWLCTIFSSVVFIQMISLFVEQGAAMDTLVVSNFHIPPASM 449
           ++C VTQVEEVKC+LRLLP+WLCTI  SVVF QM SLFV QGAAM T  + NF IP +SM
Sbjct: 324 RLCSVTQVEEVKCVLRLLPIWLCTILYSVVFTQMASLFVVQGAAMKT-NIKNFRIPASSM 383

Query: 450 TAFDIVSTTMFIMLYDRLLVPLYVRIAK-RKPKAPNELQRIGIGLAIAIVALVIAGFVEQ 509
           ++FDI+S   FI  Y R L PL+ R+ K  + K   ELQR+GIGL IAI+A++ AG VE 
Sbjct: 384 SSFDILSVAFFIFAYRRFLDPLFARLNKTERNKGLTELQRMGIGLVIAIMAMISAGIVEI 443

Query: 510 KRLKH----AGVSGKEISSFSIFWQTPQYVLVGVAEAFVYVAQMDFFTSQIPEGLKSLGM 569
            RLK+    +  S    S+ SIFWQ PQY+L+G +E F+YV Q++FF SQ P GLKS   
Sbjct: 444 HRLKNKEPESATSISSSSTLSIFWQVPQYMLIGASEVFMYVGQLEFFNSQAPTGLKSFAS 503

Query: 570 GLSMSSTAMGSYVASLILTTVMAITKTNGNPGWVPPNLNDGHLDRFFFLSAALTAFNLAL 629
            L M+S ++G+YV+SL+++ VM I+ T+   GW+P NLN GHL+RF+FL A LTA +  +
Sbjct: 504 ALCMASISLGNYVSSLLVSIVMKISTTDDVHGWIPENLNKGHLERFYFLLAGLTAADFVV 563

Query: 630 YIVCAKQYK 633
           Y++CAK YK
Sbjct: 564 YLICAKWYK 571

BLAST of Cucsa.362690 vs. Swiss-Prot
Match: PTR51_ARATH (Protein NRT1/ PTR FAMILY 7.1 OS=Arabidopsis thaliana GN=NPF7.1 PE=2 SV=1)

HSP 1 Score: 530.0 bits (1364), Expect = 3.6e-149
Identity = 274/545 (50.28%), Postives = 367/545 (67.34%), Query Frame = 1

Query: 93  RKVGGWNCGMLLLVNQGLITLAFAGVEVNLVLFSKSVLRQTNAEAANMFSRWMGTTYCFS 152
           +K GGW   ++LLVNQGL TLAF GV VNLVLF   V+ Q NAEAAN  S+W GT Y FS
Sbjct: 58  KKNGGWTNAIILLVNQGLATLAFFGVGVNLVLFLTRVMGQGNAEAANNVSKWTGTVYMFS 117

Query: 153 LLGAFLSDSYLGRYLTCVIFQLVFIVGLIALSLLTHISLLKPNGCGKIGQLCDPHSLTEL 212
           L+GAFLSDSY GRYLTC IFQ++F++G+  LS ++   L+KP GCG     C+P S   +
Sbjct: 118 LVGAFLSDSYWGRYLTCTIFQVIFVIGVGLLSFVSWFFLIKPRGCGDGDLECNPPSSLGV 177

Query: 213 SIFYISIYLIALGNGAPEPALATFGVEQFDDKDPKENRAKTSFYSYFYVSINMGCLIAET 272
           +IFY+S+YL+A G G  +P LATFG +Q DD    +  +K +F+SYFY ++N+G L + T
Sbjct: 178 AIFYLSVYLVAFGYGGHQPTLATFGADQLDD----DKNSKAAFFSYFYFALNVGALFSNT 237

Query: 273 ILVYIENLGHWMVGFWICAVCGLLAYLLFLSGTPRYRQSKPCGNPISRFSQVLMASVKKM 332
           ILVY E+ G W  GF +     ++A + FL+ T +YR  KPCGNP+ R +QV +A+ +K 
Sbjct: 238 ILVYFEDKGLWTEGFLVSLGSAIVALVAFLAPTRQYRYVKPCGNPLPRVAQVFVATARKW 297

Query: 333 NLPMPSNGEGLYEGLGK----KGTRRMLHTEGFKFLDRAAILTAEEANLICNHGQTPNPW 392
           ++  P +   LYE  G     KG+R++ H+  F FLDRAA++T  + N     G   N W
Sbjct: 298 SVVRPGDPHELYELEGPESAIKGSRKIFHSTKFLFLDRAAVITENDRN-----GTRSNAW 357

Query: 393 KICPVTQVEEVKCILRLLPVWLCTIFSSVVFIQMISLFVEQGAAMDTLVVSNFHIPPASM 452
           ++C VTQVEE KC+++LLP+WLCTI  SV+F QM SLFVEQG  M+   V  FHIP ASM
Sbjct: 358 RLCSVTQVEEAKCVMKLLPIWLCTIIYSVIFTQMASLFVEQGDVMNA-YVGKFHIPAASM 417

Query: 453 TAFDIVSTTMFIMLYDRLLVPLYVRIAKRKPKAPNELQRIGIGLAIAIVALVIAGFVEQK 512
           + FDI S  +   +Y  ++ P YVR        P EL R+GIGL I I+A+V AG  E +
Sbjct: 418 SVFDIFSVFVSTGIYRHIIFP-YVR--------PTELMRMGIGLIIGIMAMVAAGLTEIQ 477

Query: 513 RLKHAGVSGKEISSFSIFWQTPQYVLVGVAEAFVYVAQMDFFTSQIPEGLKSLGMGLSMS 572
           RLK   V G++ S  +I WQ PQYVLVG +E F+YV Q++FF  Q P+GLK+LG  L M+
Sbjct: 478 RLKRV-VPGQKESELTILWQIPQYVLVGASEVFMYVGQLEFFNGQAPDGLKNLGSSLCMA 537

Query: 573 STAMGSYVASLILTTVMAITKTNGN-PGWVPPNLNDGHLDRFFFLSAALTAFNLALYIVC 632
           S A+G+YV+SL++  VMAITK   N PGW+P NLN+GH+DRF+FL AAL A +  +Y++ 
Sbjct: 538 SMALGNYVSSLMVNIVMAITKRGENSPGWIPENLNEGHMDRFYFLIAALAAIDFVVYLIF 582

BLAST of Cucsa.362690 vs. Swiss-Prot
Match: PTR1_ARATH (Protein NRT1/ PTR FAMILY 8.1 OS=Arabidopsis thaliana GN=NPF8.1 PE=1 SV=1)

HSP 1 Score: 434.1 bits (1115), Expect = 2.7e-120
Identity = 224/554 (40.43%), Postives = 347/554 (62.64%), Query Frame = 1

Query: 87  KTTANRRKVGGWNCGMLLLVNQGLITLAFAGVEVNLVLFSKSVLRQTNAEAANMFSRWMG 146
           K  AN+ K G W     +L N+    LA+ G+  NLV + +S L Q NA AAN  + W G
Sbjct: 17  KNPANKEKTGNWKACRFILGNECCERLAYYGMGTNLVNYLESRLNQGNATAANNVTNWSG 76

Query: 147 TTYCFSLLGAFLSDSYLGRYLTCVIFQLVFIVGLIALSLLTHISLLKPNGCGKIGQLCDP 206
           T Y   L+GAF++D+YLGRY T   F  +++ G+  L+L   +  LKP  C      C P
Sbjct: 77  TCYITPLIGAFIADAYLGRYWTIATFVFIYVSGMTLLTLSASVPGLKPGNCN--ADTCHP 136

Query: 207 HSLTELSIFYISIYLIALGNGAPEPALATFGVEQFDDKDPKENRAKTSFYSYFYVSINMG 266
           +S ++ ++F++++Y+IALG G  +P +++FG +QFD+ D  E   K+SF+++FY SIN+G
Sbjct: 137 NS-SQTAVFFVALYMIALGTGGIKPCVSSFGADQFDENDENEKIKKSSFFNWFYFSINVG 196

Query: 267 CLIAETILVYIENLGHWMVGFWICAVCGLLAYLLFLSGTPRYRQSKPCGNPISRFSQVLM 326
            LIA T+LV+I+    W  GF +  V  ++A   F  G+  YR  +P G+P++R  QV++
Sbjct: 197 ALIAATVLVWIQMNVGWGWGFGVPTVAMVIAVCFFFFGSRFYRLQRPGGSPLTRIFQVIV 256

Query: 327 ASVKKMNLPMPSNGEGLYEGLGK----KGTRRMLHTEGFKFLDRAAILTAEEANLICNHG 386
           A+ +K+++ +P +   L+E        KG+R+++HT+  KF D+AA+ +  ++      G
Sbjct: 257 AAFRKISVKVPEDKSLLFETADDESNIKGSRKLVHTDNLKFFDKAAVESQSDS---IKDG 316

Query: 387 QTPNPWKICPVTQVEEVKCILRLLPVWLCTIFSSVVFIQMISLFVEQGAAMDTLVVSNFH 446
           +  NPW++C VTQVEE+K I+ LLPVW   I  + V+ QM ++FV QG  MD  +  NF 
Sbjct: 317 EV-NPWRLCSVTQVEELKSIITLLPVWATGIVFATVYSQMSTMFVLQGNTMDQHMGKNFE 376

Query: 447 IPPASMTAFDIVSTTMFIMLYDRLLVPLYVRIAKRKPKAPNELQRIGIGLAIAIVALVIA 506
           IP AS++ FD VS   +  +YD+ ++PL  R   R  +   +LQR+GIGL ++I A++ A
Sbjct: 377 IPSASLSLFDTVSVLFWTPVYDQFIIPL-ARKFTRNERGFTQLQRMGIGLVVSIFAMITA 436

Query: 507 GFVEQKRLK----HAGVSGKEISSFSIFWQTPQYVLVGVAEAFVYVAQMDFFTSQIPEGL 566
           G +E  RL     H     K+I   SIFWQ PQY+L+G AE F ++ Q++FF  Q P+ +
Sbjct: 437 GVLEVVRLDYVKTHNAYDQKQIH-MSIFWQIPQYLLIGCAEVFTFIGQLEFFYDQAPDAM 496

Query: 567 KSLGMGLSMSSTAMGSYVASLILTTVMAITKTNGNPGWVPPNLNDGHLDRFFFLSAALTA 626
           +SL   LS+++ A+G+Y++++++T VM ITK NG PGW+P NLN GHLD FF+L A L+ 
Sbjct: 497 RSLCSALSLTTVALGNYLSTVLVTVVMKITKKNGKPGWIPDNLNRGHLDYFFYLLATLSF 556

Query: 627 FNLALYIVCAKQYK 633
            N  +Y+  +K+YK
Sbjct: 557 LNFLVYLWISKRYK 561

BLAST of Cucsa.362690 vs. Swiss-Prot
Match: PTR17_ARATH (Protein NRT1/ PTR FAMILY 8.5 OS=Arabidopsis thaliana GN=NPF8.5 PE=2 SV=1)

HSP 1 Score: 431.4 bits (1108), Expect = 1.7e-119
Identity = 226/566 (39.93%), Postives = 351/566 (62.01%), Query Frame = 1

Query: 73  SSSLEDGALTDQNDKTTANRRKVGGWNCGMLLLVNQGLITLAFAGVEVNLVLFSKSVLRQ 132
           SS+ EDG++    +  +  ++K G W     +L N+    LA+ G+  NL+ +  S L +
Sbjct: 33  SSTAEDGSIDIYGNPPS--KKKTGNWKACPFILGNECCERLAYYGIAKNLITYYTSELHE 92

Query: 133 TNAEAANMFSRWMGTTYCFSLLGAFLSDSYLGRYLTCVIFQLVFIVGLIALSLLTHISLL 192
           +N  AA+    W GT Y   L+GA ++DSY GRY T   F  ++ +G+  L+L   + +L
Sbjct: 93  SNVSAASDVMIWQGTCYITPLIGAVIADSYWGRYWTIASFSAIYFIGMALLTLSASLPVL 152

Query: 193 KPNGC-GKIGQLCDPHSLTELSIFYISIYLIALGNGAPEPALATFGVEQFDDKDPKENRA 252
           KP  C G    LC P +  + ++F+  +YLIALG G  +P +++FG +QFDD DP+E   
Sbjct: 153 KPAACAGVAAALCSPATTVQYAVFFTGLYLIALGTGGIKPCVSSFGADQFDDTDPRERVR 212

Query: 253 KTSFYSYFYVSINMGCLIAETILVYIENLGHWMVGFWICAVCGLLAYLLFLSGTPRYRQS 312
           K SF+++FY SIN+G  I+ T+LV+++    W +GF I  V   ++   F  GTP YR  
Sbjct: 213 KASFFNWFYFSINIGSFISSTLLVWVQENVGWGLGFLIPTVFMGVSIASFFIGTPLYRFQ 272

Query: 313 KPCGNPISRFSQVLMASVKKMNLPMPSNGEGLYEGLGKK----GTRRMLHTEGFKFLDRA 372
           KP G+PI+R  QVL+A+ +K+ L +P +   LYE   K     G+R++ HT+G+KFLD+A
Sbjct: 273 KPGGSPITRVCQVLVAAYRKLKLNLPEDISFLYETREKNSMIAGSRKIQHTDGYKFLDKA 332

Query: 373 AILTAEEANLICNHGQTPNPWKICPVTQVEEVKCILRLLPVWLCTIFSSVVFIQMISLFV 432
           A+++  E+      G   NPWK+C VTQVEEVK ++R+ P+W   I  SV++ Q+ +LFV
Sbjct: 333 AVISEYES----KSGAFSNPWKLCTVTQVEEVKTLIRMFPIWASGIVYSVLYSQISTLFV 392

Query: 433 EQGAAMDTLVVSNFHIPPASMTAFDIVSTTMFIMLYDRLLVPLYVRIAKRKPKAPNELQR 492
           +QG +M+  ++ +F IPPAS   FD +   + I +YDR LVP +VR     PK   +LQR
Sbjct: 393 QQGRSMNR-IIRSFEIPPASFGVFDTLIVLISIPIYDRFLVP-FVRRFTGIPKGLTDLQR 452

Query: 493 IGIGLAIAIVALVIAGFVEQKRLKHAGVSGKEISSFSIFWQTPQYVLVGVAEAFVYVAQM 552
           +GIGL ++++++  A  VE  RL+ A    ++  + SIFWQ PQY+L+G+AE F ++ ++
Sbjct: 453 MGIGLFLSVLSIAAAAIVETVRLQLA----QDFVAMSIFWQIPQYILMGIAEVFFFIGRV 512

Query: 553 DFFTSQIPEGLKSLGMGLSMSSTAMGSYVASLILTTVMAITKTNGNPGWVPPNLNDGHLD 612
           +FF  + P+ ++S+   L++ +TA+GSY++SLILT V   T   G  GWVP +LN GHLD
Sbjct: 513 EFFYDESPDAMRSVCSALALLNTAVGSYLSSLILTLVAYFTALGGKDGWVPDDLNKGHLD 572

Query: 613 RFFFLSAALTAFNLALY-IVCAKQYK 633
            FF+L  +L   N+ +Y ++C K  K
Sbjct: 573 YFFWLLVSLGLVNIPVYALICVKHTK 586

BLAST of Cucsa.362690 vs. TrEMBL
Match: A0A0A0KEP8_CUCSA (Uncharacterized protein OS=Cucumis sativus GN=Csa_6G077970 PE=4 SV=1)

HSP 1 Score: 1190.6 bits (3079), Expect = 0.0e+00
Identity = 595/596 (99.83%), Postives = 596/596 (100.00%), Query Frame = 1

Query: 57  QVADLHLLENGQERFQSSSLEDGALTDQNDKTTANRRKVGGWNCGMLLLVNQGLITLAFA 116
           +VADLHLLENGQERFQSSSLEDGALTDQNDKTTANRRKVGGWNCGMLLLVNQGLITLAFA
Sbjct: 4   KVADLHLLENGQERFQSSSLEDGALTDQNDKTTANRRKVGGWNCGMLLLVNQGLITLAFA 63

Query: 117 GVEVNLVLFSKSVLRQTNAEAANMFSRWMGTTYCFSLLGAFLSDSYLGRYLTCVIFQLVF 176
           GVEVNLVLFSKSVLRQTNAEAANMFSRWMGTTYCFSLLGAFLSDSYLGRYLTCVIFQLVF
Sbjct: 64  GVEVNLVLFSKSVLRQTNAEAANMFSRWMGTTYCFSLLGAFLSDSYLGRYLTCVIFQLVF 123

Query: 177 IVGLIALSLLTHISLLKPNGCGKIGQLCDPHSLTELSIFYISIYLIALGNGAPEPALATF 236
           IVGLIALSLLTHISLLKPNGCGKIGQLCDPHSLTELSIFYISIYLIALGNGAPEPALATF
Sbjct: 124 IVGLIALSLLTHISLLKPNGCGKIGQLCDPHSLTELSIFYISIYLIALGNGAPEPALATF 183

Query: 237 GVEQFDDKDPKENRAKTSFYSYFYVSINMGCLIAETILVYIENLGHWMVGFWICAVCGLL 296
           GVEQFDDKDPKENRAKTSFYSYFYVSINMGCLIAETILVYIENLGHWMVGFWICAVCGLL
Sbjct: 184 GVEQFDDKDPKENRAKTSFYSYFYVSINMGCLIAETILVYIENLGHWMVGFWICAVCGLL 243

Query: 297 AYLLFLSGTPRYRQSKPCGNPISRFSQVLMASVKKMNLPMPSNGEGLYEGLGKKGTRRML 356
           AYLLFLSGTPRYRQSKPCGNPISRFSQVLMASVKKMNLPMPSNGEGLYEGLGKKGTRRML
Sbjct: 244 AYLLFLSGTPRYRQSKPCGNPISRFSQVLMASVKKMNLPMPSNGEGLYEGLGKKGTRRML 303

Query: 357 HTEGFKFLDRAAILTAEEANLICNHGQTPNPWKICPVTQVEEVKCILRLLPVWLCTIFSS 416
           HTEGFKFLDRAAILTAEEANLICNHGQTPNPWKICPVTQVEEVKCILRLLPVWLCTIFSS
Sbjct: 304 HTEGFKFLDRAAILTAEEANLICNHGQTPNPWKICPVTQVEEVKCILRLLPVWLCTIFSS 363

Query: 417 VVFIQMISLFVEQGAAMDTLVVSNFHIPPASMTAFDIVSTTMFIMLYDRLLVPLYVRIAK 476
           VVFIQMISLFVEQGAAMDTLVVSNFHIPPASMTAFDIVSTTMFIMLYDRLLVPLYVRIAK
Sbjct: 364 VVFIQMISLFVEQGAAMDTLVVSNFHIPPASMTAFDIVSTTMFIMLYDRLLVPLYVRIAK 423

Query: 477 RKPKAPNELQRIGIGLAIAIVALVIAGFVEQKRLKHAGVSGKEISSFSIFWQTPQYVLVG 536
           RKPKAPNELQRIGIGLAIAIVALVIAGFVEQKRLKHAGVSGKEISSFSIFWQTPQYVLVG
Sbjct: 424 RKPKAPNELQRIGIGLAIAIVALVIAGFVEQKRLKHAGVSGKEISSFSIFWQTPQYVLVG 483

Query: 537 VAEAFVYVAQMDFFTSQIPEGLKSLGMGLSMSSTAMGSYVASLILTTVMAITKTNGNPGW 596
           VAEAFVYVAQMDFFTSQIPEGLKSLGMGLSMSSTAMGSYVASLILTTVMAITKTNGNPGW
Sbjct: 484 VAEAFVYVAQMDFFTSQIPEGLKSLGMGLSMSSTAMGSYVASLILTTVMAITKTNGNPGW 543

Query: 597 VPPNLNDGHLDRFFFLSAALTAFNLALYIVCAKQYKGSIIEKQDGDQEEEPDREFK 653
           VPPNLNDGHLDRFFFLSAALTAFNLALYIVCAKQYKGSIIEKQDGDQEEEPDREFK
Sbjct: 544 VPPNLNDGHLDRFFFLSAALTAFNLALYIVCAKQYKGSIIEKQDGDQEEEPDREFK 599

BLAST of Cucsa.362690 vs. TrEMBL
Match: W9RGT0_9ROSA (Nitrate transporter 1.5 OS=Morus notabilis GN=L484_025587 PE=4 SV=1)

HSP 1 Score: 827.8 bits (2137), Expect = 9.3e-237
Identity = 409/610 (67.05%), Postives = 502/610 (82.30%), Query Frame = 1

Query: 48  PDLKSN------DELQVADLHLLENGQERFQSSSLEDGALTDQNDKTTANRRKVGGWNCG 107
           P+  SN      +  ++ +L+ LENGQER +++  +DG+L D++ K  A + + GGW CG
Sbjct: 3   PEASSNGTSVLCNNFKMVELNQLENGQERLENACTKDGSL-DRHGKP-ALKGRTGGWRCG 62

Query: 108 MLLLVNQGLITLAFAGVEVNLVLFSKSVLRQTNAEAANMFSRWMGTTYCFSLLGAFLSDS 167
           MLLLVNQGLITLAFAGVEVNLVLFSKSVLR +NAEAA+ FS WMGT Y FSL+GAFLSDS
Sbjct: 63  MLLLVNQGLITLAFAGVEVNLVLFSKSVLRHSNAEAAHTFSTWMGTVYLFSLMGAFLSDS 122

Query: 168 YLGRYLTCVIFQLVFIVGLIALSLLTHISLLKPNGCGKIGQLCDPHSLTELSIFYISIYL 227
           YLGRY+TCVIFQ+V+I+GLI LS+  ++ L++P+GCGKIG++C+PH   E +IFY+SIYL
Sbjct: 123 YLGRYITCVIFQVVYIIGLIVLSMSANLFLIRPHGCGKIGEVCEPHKPLESAIFYMSIYL 182

Query: 228 IALGNGAPEPALATFGVEQFDDKDPKENRAKTSFYSYFYVSINMGCLIAETILVYIENLG 287
           IALGNGA EPALATFG +QFD++D KE R+K SFYSYFYV++N+G L+AETILVYIEN+G
Sbjct: 183 IALGNGASEPALATFGADQFDEEDQKEKRSKASFYSYFYVALNLGSLVAETILVYIENMG 242

Query: 288 HWMVGFWICAVCGLLAYLLFLSGTPRYRQSKPCGNPISRFSQVLMASVKKMNLPMPSNGE 347
           +W+V FW C +  ++ Y+  LSGT RYR  KP GNPISRFSQV++AS++K++  +PSN E
Sbjct: 243 YWVVAFWTCTISAVVGYVFLLSGTLRYRHFKPSGNPISRFSQVIVASMRKLSQNLPSNEE 302

Query: 348 GLYEGLGKK----GTRRMLHTEGFKFLDRAAILTAEEANLICNHGQTPNPWKICPVTQVE 407
            LYE  GK+    G RR+LHT+GFKFLDRAAI+  E+  LI N  +TPNPW +C VTQVE
Sbjct: 303 ELYEVHGKEGETNGMRRILHTQGFKFLDRAAIIMPEDMKLIRNEARTPNPWHLCTVTQVE 362

Query: 408 EVKCILRLLPVWLCTIFSSVVFIQMISLFVEQGAAMDTLVVSNFHIPPASMTAFDIVSTT 467
           EVKC+LRLLPVWLCTIFSSVVFIQM+SLFVEQGAAM+   VSNFHIPPASMT FDIVST+
Sbjct: 363 EVKCVLRLLPVWLCTIFSSVVFIQMLSLFVEQGAAMNR-TVSNFHIPPASMTVFDIVSTS 422

Query: 468 MFIMLYDRLLVPLYVRIAKRKPKAPNELQRIGIGLAIAIVALVIAGFVEQKRLKH-AGVS 527
           +FIMLYD+++VPLY+++ KR+PK P+ LQRIGIGL IA++A++IAG VEQ+RL++     
Sbjct: 423 VFIMLYDKIIVPLYIKLTKREPKIPSPLQRIGIGLTIAVLAMIIAGIVEQQRLRYTTNFV 482

Query: 528 GKEISSFSIFWQTPQYVLVGVAEAFVYVAQMDFFTSQIPEGLKSLGMGLSMSSTAMGSYV 587
            KEISS SI WQTPQYVLVGV+EAFVYVAQM+FF SQ P+GLKSLG+GLSMSS+AMGSYV
Sbjct: 483 RKEISSLSILWQTPQYVLVGVSEAFVYVAQMEFFASQTPDGLKSLGIGLSMSSSAMGSYV 542

Query: 588 ASLILTTVMAITKTNGNPGWVPPNLNDGHLDRFFFLSAALTAFNLALYIVCAKQYKGSII 647
           AS ILT VM IT  NG PGWVPPNLNDGHLDRFFF+SAAL A N  LY+VCAK+YK   +
Sbjct: 543 ASTILTIVMKITSKNGKPGWVPPNLNDGHLDRFFFVSAALAALNFLLYVVCAKRYKSISL 602

BLAST of Cucsa.362690 vs. TrEMBL
Match: M5X2X3_PRUPE (Uncharacterized protein (Fragment) OS=Prunus persica GN=PRUPE_ppa017670mg PE=4 SV=1)

HSP 1 Score: 812.8 bits (2098), Expect = 3.1e-232
Identity = 399/579 (68.91%), Postives = 485/579 (83.77%), Query Frame = 1

Query: 65  ENGQERFQSSSLEDGALTDQNDKTTANRRKVGGWNCGMLLLVNQGLITLAFAGVEVNLVL 124
           + G++R  ++   DG++ D   K  A +   GGW CGMLLLVNQGL++LAFAGVEVN+VL
Sbjct: 8   KGGEKRLLNACTRDGSV-DWYGKP-ALKGSTGGWRCGMLLLVNQGLVSLAFAGVEVNMVL 67

Query: 125 FSKSVLRQTNAEAANMFSRWMGTTYCFSLLGAFLSDSYLGRYLTCVIFQLVFIVGLIALS 184
           F+KSVLRQTNAEAAN FSRWMGT Y  SL+GA LSDSYLGRYLTCV FQ+V  +GLIALS
Sbjct: 68  FAKSVLRQTNAEAANTFSRWMGTFYFCSLIGALLSDSYLGRYLTCVTFQIVHTIGLIALS 127

Query: 185 LLTHISLLKPNGCGKIGQLCDPHSLTELSIFYISIYLIALGNGAPEPALATFGVEQFDDK 244
           L T++ LL+P GCG+IGQLC+PH   E+++FYISIYLIALGNGAPEPALA FG +QFD++
Sbjct: 128 LSTNLFLLRPPGCGEIGQLCEPHRPMEVAMFYISIYLIALGNGAPEPALAAFGADQFDEE 187

Query: 245 DPKENRAKTSFYSYFYVSINMGCLIAETILVYIENLGHWMVGFWICAVCGLLAYLLFLSG 304
           D +E +AKTSFYSYFYV++N+GCL+AET+LVY+E++GHW+  FWIC    ++  +LFL G
Sbjct: 188 DSEEKQAKTSFYSYFYVALNLGCLVAETVLVYMESMGHWVPAFWICTGSSVVGSVLFLGG 247

Query: 305 TPRYRQSKPCGNPISRFSQVLMASVKKMNLPMPSNGEGLYEGLGK----KGTRRMLHTEG 364
           + RYR  K  GNPISRFSQV++AS +K+N  +PSNGEGLYE +G+     G+RR+LHT+G
Sbjct: 248 SCRYRHFKASGNPISRFSQVIVASTRKINHELPSNGEGLYETIGRGSETNGSRRILHTKG 307

Query: 365 FKFLDRAAILTAEEANLICNHGQTPNPWKICPVTQVEEVKCILRLLPVWLCTIFSSVVFI 424
           FKFLDRAA +T ++ +L+ N GQTPNPW +C VTQVEEVKCILRLLPVWLCT+ SS+VFI
Sbjct: 308 FKFLDRAAFITCDDISLMSNQGQTPNPWHLCTVTQVEEVKCILRLLPVWLCTVLSSMVFI 367

Query: 425 QMISLFVEQGAAMDTLVVSNFHIPPASMTAFDIVSTTMFIMLYDRLLVPLYVRIAKRKPK 484
           Q++SLFVEQGAAMD   VSNF IPPASMTAFDIVST++FI+LYD+L+VPLY ++ KR+PK
Sbjct: 368 QVLSLFVEQGAAMDRR-VSNFQIPPASMTAFDIVSTSLFIILYDKLIVPLYTKVTKREPK 427

Query: 485 APNELQRIGIGLAIAIVALVIAGFVEQKRLKHAGVSGKEISSFSIFWQTPQYVLVGVAEA 544
            P+ LQRIGIGL I IV LVIAGFVE+KRLK+A  SG+E SS SIFWQTPQYVLVGV+EA
Sbjct: 428 PPSALQRIGIGLTIGIVGLVIAGFVERKRLKYASNSGEETSSLSIFWQTPQYVLVGVSEA 487

Query: 545 FVYVAQMDFFTSQIPEGLKSLGMGLSMSSTAMGSYVASLILTTVMAITKTNGNPGWVPPN 604
           FVYV+QM+F++SQ P+GLKSLG+ L M+S+AMGSYVAS+ILT VM IT  NG PGWVPPN
Sbjct: 488 FVYVSQMEFYSSQTPDGLKSLGIALWMTSSAMGSYVASMILTVVMEITTKNGQPGWVPPN 547

Query: 605 LNDGHLDRFFFLSAALTAFNLALYIVCAKQYKGSIIEKQ 640
           LNDGHLDRFFFLSA LTA NLALYIVCAK+YK   +EK+
Sbjct: 548 LNDGHLDRFFFLSAGLTALNLALYIVCAKRYKSISLEKR 583

BLAST of Cucsa.362690 vs. TrEMBL
Match: A0A061E1X8_THECC (Nitrate transporter 1.5 OS=Theobroma cacao GN=TCM_005536 PE=4 SV=1)

HSP 1 Score: 792.3 bits (2045), Expect = 4.3e-226
Identity = 384/592 (64.86%), Postives = 487/592 (82.26%), Query Frame = 1

Query: 62  HLLENGQERF---QSSSLEDGALTDQNDKTTANRRKVGGWNCGMLLLVNQGLITLAFAGV 121
           +++E+  ER    +++  +DG+L D++ K  A + + GGW C M +LVNQGL TLAFA V
Sbjct: 20  YVIESNDERKRPDENACTKDGSL-DRHGKP-ATKGRTGGWRCAMFILVNQGLATLAFAAV 79

Query: 122 EVNLVLFSKSVLRQTNAEAANMFSRWMGTTYCFSLLGAFLSDSYLGRYLTCVIFQLVFIV 181
           EVNLVLFSKSVLR TNAEAA+ FSRWMGT Y FS +GAFLSDSYLGRYLTCV FQ+++++
Sbjct: 80  EVNLVLFSKSVLRHTNAEAADTFSRWMGTVYLFSFMGAFLSDSYLGRYLTCVTFQVLYLI 139

Query: 182 GLIALSLLTHISLLKPNGCGKIGQLCDPHSLTELSIFYISIYLIALGNGAPEPALATFGV 241
           GL+ALSL+T + LLKP GCGK+GQLC+PHS  E++IFY++IYLIALGNGAPEPALA FG 
Sbjct: 140 GLVALSLVTQLFLLKPQGCGKLGQLCEPHSPAEIAIFYVAIYLIALGNGAPEPALAAFGA 199

Query: 242 EQFDDKDPKENRAKTSFYSYFYVSINMGCLIAETILVYIENLGHWMVGFWICAVCGLLAY 301
           +QFD++D +E ++K SFYSYFYV++N+G L+AET+LVY++N+G+W++GFWICA C ++A+
Sbjct: 200 DQFDEEDDEEKQSKNSFYSYFYVALNLGSLVAETVLVYVQNIGNWILGFWICATCAMVAF 259

Query: 302 LLFLSGTPRYRQSKPCGNPISRFSQVLMASVKKMNLPMPSNGEGLYEGLGKK----GTRR 361
            L L GT RYR  K  GNPIS+F QV++AS++K+NL +PS+ EGLYE   K+    G RR
Sbjct: 260 SLLLCGTFRYRHFKTVGNPISKFCQVIVASMRKINLQVPSDVEGLYEVQRKEDKNNGVRR 319

Query: 362 MLHTEGFKFLDRAAILTAEEANLICNHGQTPNPWKICPVTQVEEVKCILRLLPVWLCTIF 421
           +LHT+GFKFLDRAAI+T ++  L+ N G+T NPW +C +TQVEEVKCILRLLP+WLC+I 
Sbjct: 320 ILHTDGFKFLDRAAIITPQDIFLLTNKGETANPWYLCTITQVEEVKCILRLLPIWLCSIL 379

Query: 422 SSVVFIQMISLFVEQGAAMDTLVVSNFHIPPASMTAFDIVSTTMFIMLYDRLLVPLYVRI 481
           SSVVF+QM+SLFVEQGAAMDT  +SNFHIPPASM AFDIVST+ FI+LYD+L+VPLYVR+
Sbjct: 380 SSVVFVQMLSLFVEQGAAMDT-TISNFHIPPASMFAFDIVSTSAFILLYDKLMVPLYVRL 439

Query: 482 AKRKPKAPNELQRIGIGLAIAIVALVIAGFVEQKRLKHAGVSGKEISSFSIFWQTPQYVL 541
            KR+PK P+ LQRIGIG+AIA+V+++IAG VEQ+RLK+A  +G E S+ SIFWQTPQYVL
Sbjct: 440 TKREPKPPSGLQRIGIGIAIAMVSMIIAGTVEQQRLKYANHNGAETSALSIFWQTPQYVL 499

Query: 542 VGVAEAFVYVAQMDFFTSQIPEGLKSLGMGLSMSSTAMGSYVASLILTTVMAITKTNGNP 601
           VGV+EA +YVAQM+FF SQ P+GLKSLG+GLSM S+AMGSY+ S+IL  VMAIT  NG P
Sbjct: 500 VGVSEALIYVAQMEFFASQTPDGLKSLGIGLSMYSSAMGSYICSIILAAVMAITSKNGKP 559

Query: 602 GWVPPNLNDGHLDRFFFLSAALTAFNLALYIVCAKQYKGSIIEKQDGDQEEE 647
           GWVPP+LN GHLDR+FFL AAL A NLAL++ CAK+Y     EK+    E E
Sbjct: 560 GWVPPDLNAGHLDRYFFLLAALAALNLALFVACAKRYNPVSFEKRAEGVEME 608

BLAST of Cucsa.362690 vs. TrEMBL
Match: V4U368_9ROSI (Uncharacterized protein (Fragment) OS=Citrus clementina GN=CICLE_v10024592mg PE=4 SV=1)

HSP 1 Score: 779.2 bits (2011), Expect = 3.8e-222
Identity = 376/541 (69.50%), Postives = 456/541 (84.29%), Query Frame = 1

Query: 106 VNQGLITLAFAGVEVNLVLFSKSVLRQTNAEAANMFSRWMGTTYCFSLLGAFLSDSYLGR 165
           VNQGL  LAFAGVEVN+VLF+KSVLRQTNAEA+N FSRWMG+ Y  SL+GAFLSDSYLGR
Sbjct: 79  VNQGLANLAFAGVEVNMVLFAKSVLRQTNAEASNTFSRWMGSLYLSSLMGAFLSDSYLGR 138

Query: 166 YLTCVIFQLVFIVGLIALSLLTHISLLKPNGCGKIGQLCDPHSLTELSIFYISIYLIALG 225
           YLTCV FQ   I+GL+ LS  T   LL+P+GCGKIGQLCDPHS  E +IFYISIYL ALG
Sbjct: 139 YLTCVTFQAFSIIGLLGLSFATQSFLLRPHGCGKIGQLCDPHSPAETAIFYISIYLFALG 198

Query: 226 NGAPEPALATFGVEQFDDKDPKENRAKTSFYSYFYVSINMGCLIAETILVYIENLGHWMV 285
            GAPEPALATFG +QFD++DPKE ++KTSFYSYFY+++N+GCL +ET+LVY EN+G+W++
Sbjct: 199 IGAPEPALATFGADQFDEEDPKEKKSKTSFYSYFYIALNLGCLFSETVLVYAENMGYWVL 258

Query: 286 GFWICAVCGLLAYLLFLSGTPRYRQSKPCGNPISRFSQVLMASVKKMNLPMPSNGEGLYE 345
           GFWICA C +LA+   LSGT RYR  KP GNP+SRFSQV++AS++K+NL MP+NG GL+E
Sbjct: 259 GFWICAGCDILAFSSLLSGTLRYRHFKPSGNPLSRFSQVIVASLRKINLEMPTNGGGLFE 318

Query: 346 ----GLGKKGTRRMLHTEGFKFLDRAAILTAEEANLICNHGQTPNPWKICPVTQVEEVKC 405
                    GTRR+LHT  FKFLDRAAI+T+  A++  N  Q PN W++C VTQVEEVKC
Sbjct: 319 VHVREYEANGTRRILHTNDFKFLDRAAIMTS--ADITTNEDQNPNQWRLCTVTQVEEVKC 378

Query: 406 ILRLLPVWLCTIFSSVVFIQMISLFVEQGAAMDTLVVSNFHIPPASMTAFDIVSTTMFIM 465
           +LRLLPVWLCTI SS+ +IQM+SLFVEQGAAM+T  VSNF IPPASMTAFDIVSTT FI+
Sbjct: 379 VLRLLPVWLCTILSSMAYIQMLSLFVEQGAAMNT-GVSNFQIPPASMTAFDIVSTTAFIL 438

Query: 466 LYDRLLVPLYVRIAKRKPKAPNELQRIGIGLAIAIVALVIAGFVEQKRLKHAGVSGKEIS 525
           LYD+L+VPLY+++AKR+PK P+ELQR+GIGLAIA VA++IAG VEQ+RLK+A   G+E S
Sbjct: 439 LYDKLIVPLYLKVAKRRPKTPSELQRVGIGLAIATVAMIIAGIVEQRRLKYASFGGEETS 498

Query: 526 SFSIFWQTPQYVLVGVAEAFVYVAQMDFFTSQIPEGLKSLGMGLSMSSTAMGSYVASLIL 585
           S SIFWQTPQYVL+GV+EAFVYVAQM+FF SQIP GLKSLG+ LSMSSTA+G+YV S+IL
Sbjct: 499 SLSIFWQTPQYVLLGVSEAFVYVAQMEFFASQIPNGLKSLGIALSMSSTAIGTYVCSMIL 558

Query: 586 TTVMAITKTNGNPGWVPPNLNDGHLDRFFFLSAALTAFNLALYIVCAKQYKGSIIEKQDG 643
           + VM +T  NG PGWVPPNLNDGHLDRFFFLSA +TA NL +++VCA++YK   ++KQ+G
Sbjct: 559 SVVMVLTTKNGKPGWVPPNLNDGHLDRFFFLSATVTAMNLGVFVVCARRYKSISLQKQEG 616

BLAST of Cucsa.362690 vs. TAIR10
Match: AT1G32450.1 (AT1G32450.1 nitrate transporter 1.5)

HSP 1 Score: 591.3 bits (1523), Expect = 7.4e-169
Identity = 303/567 (53.44%), Postives = 400/567 (70.55%), Query Frame = 1

Query: 92  RRKVGGWNCGMLLLVNQGLITLAFAGVEVNLVLFSKSVLRQTNAEAANMFSRWMGTTYCF 151
           R   G W  G+++L+NQGL TLAF GV VNLVLF   VL+Q NA+AAN  S+W GT Y F
Sbjct: 35  RSNSGQWVAGIVILLNQGLATLAFFGVGVNLVLFLTRVLQQNNADAANNVSKWTGTVYIF 94

Query: 152 SLLGAFLSDSYLGRYLTCVIFQLVFIVGLIALSLLTHISLLKPNGCGKIGQLCDPHSLTE 211
           SL+GAFLSDSY GRY TC IFQ++F++GL +LSL +++ L++P GCG     C  HS+ E
Sbjct: 95  SLVGAFLSDSYWGRYKTCAIFQVIFVIGLSSLSLSSYMFLIRPRGCGDEVTPCGSHSMME 154

Query: 212 LSIFYISIYLIALGNGAPEPALATFGVEQFDDKDPKENRAKTSFYSYFYVSINMGCLIAE 271
           +++FY SIYLIALG G  +P +AT G +QFD++ PKE  +K +F+SYFY+++N+G L + 
Sbjct: 155 ITMFYFSIYLIALGYGGYQPNIATLGADQFDEEHPKEGYSKIAFFSYFYLALNLGSLFSN 214

Query: 272 TILVYIENLGHWMVGFWICAVCGLLAYLLFLSGTPRYRQSKPCGNPISRFSQVLMASVKK 331
           TIL Y E+ G W +GFW      ++  +LFL GTPRYR  KP GNP+SRF QVL+A+ KK
Sbjct: 215 TILGYFEDEGMWALGFWASTGSAIIGLILFLVGTPRYRYFKPTGNPLSRFCQVLVAATKK 274

Query: 332 MNLPMPSNG-EGLYEG--LGKKGT----RRMLHTEGFKFLDRAAILTAEEANLICNHGQT 391
            ++  P  G E +Y+G   GK  +    RR++HT+ FKFLD+AA +TA +  L      +
Sbjct: 275 SSVEAPLRGREEMYDGDSEGKNASVNTGRRIVHTDEFKFLDKAAYITARD--LDDKKQDS 334

Query: 392 PNPWKICPVTQVEEVKCILRLLPVWLCTIFSSVVFIQMISLFVEQGAAMDTLVVSNFHIP 451
            NPW++CPVTQVEEVKCILRL+P+WLCTI  SVVF QM SLFVEQGAAM+T  VS+F IP
Sbjct: 335 VNPWRLCPVTQVEEVKCILRLMPIWLCTIIYSVVFTQMASLFVEQGAAMNT-SVSDFKIP 394

Query: 452 PASMTAFDIVSTTMFIMLYDRLLVPLYVRIAKRKPKAPNELQRIGIGLAIAIVALVIAGF 511
           PASM++FDI+S  +FI LY R+L P+  R  K   K   EL R+GIGL IA++A++ AG 
Sbjct: 395 PASMSSFDILSVALFIFLYRRVLEPVANRFKKNGSKGITELHRMGIGLVIAVIAMIAAGI 454

Query: 512 VEQKRLKHAGVSGKEI---SSFSIFWQTPQYVLVGVAEAFVYVAQMDFFTSQIPEGLKSL 571
           VE  RLK+A  S       SS SIFWQ PQY L+G +E F+YV Q++FF +Q P+GLKS 
Sbjct: 455 VECYRLKYADKSCTHCDGSSSLSIFWQAPQYSLIGASEVFMYVGQLEFFNAQTPDGLKSF 514

Query: 572 GMGLSMSSTAMGSYVASLILTTVMAITKTNGNPGWVPPNLNDGHLDRFFFLSAALTAFNL 631
           G  L M S +MG++V+SL++T V+ I+  +  PGW+P NLN GHLDRF+FL AALT+ +L
Sbjct: 515 GSALCMMSMSMGNFVSSLLVTMVVKISTEDHMPGWIPRNLNKGHLDRFYFLLAALTSIDL 574

Query: 632 ALYIVCAKQYKGSIIEKQDGDQEEEPD 649
            +YI CAK YK   +E +D  Q+   D
Sbjct: 575 VVYIACAKWYKPIQLEGKDEMQDMSDD 598

BLAST of Cucsa.362690 vs. TAIR10
Match: AT4G21680.1 (AT4G21680.1 NITRATE TRANSPORTER 1.8)

HSP 1 Score: 553.9 bits (1426), Expect = 1.3e-157
Identity = 285/549 (51.91%), Postives = 373/549 (67.94%), Query Frame = 1

Query: 90  ANRRKVGGWNCGMLLLVNQGLITLAFAGVEVNLVLFSKSVLRQTNAEAANMFSRWMGTTY 149
           A R   G W   +L+LVNQGL TLAF GV VNLVLF   V+ Q NAEAAN  S+W GT Y
Sbjct: 24  AIRANTGKWLTAILILVNQGLATLAFFGVGVNLVLFLTRVMGQDNAEAANNVSKWTGTVY 83

Query: 150 CFSLLGAFLSDSYLGRYLTCVIFQLVFIVGLIALSLLTHISLLKPNGCGKIGQLCDPHSL 209
            FSLLGAFLSDSY GRY TC IFQ  F+ GL+ LSL T   LL+P+GCG     C PHS 
Sbjct: 84  IFSLLGAFLSDSYWGRYKTCAIFQASFVAGLMMLSLSTGALLLEPSGCGVEDSPCKPHST 143

Query: 210 TELSIFYISIYLIALGNGAPEPALATFGVEQFDDKDPKENRAKTSFYSYFYVSINMGCLI 269
            +  +FY+S+YLIALG G  +P +ATFG +QFD +D  E  +K +F+SYFY+++N+G L 
Sbjct: 144 FKTVLFYLSVYLIALGYGGYQPNIATFGADQFDAEDSVEGHSKIAFFSYFYLALNLGSLF 203

Query: 270 AETILVYIENLGHWMVGFWICAVCGLLAYLLFLSGTPRYRQSKPCGNPISRFSQVLMASV 329
           + T+L Y E+ G W +GFW  A       +LFL GTP+YR   P  +P SRF QVL+A+ 
Sbjct: 204 SNTVLGYFEDQGEWPLGFWASAGSAFAGLVLFLIGTPKYRHFTPRESPWSRFCQVLVAAT 263

Query: 330 KKMNLPMPSNGEGLYEGLGK-KGTRRMLHTEGFKFLDRAAILTAEEANLICNHGQTPNPW 389
           +K  + +      LY+   +  G +++LHT+GF+FLDRAAI+T ++       G   +PW
Sbjct: 264 RKAKIDVHHEELNLYDSETQYTGDKKILHTKGFRFLDRAAIVTPDDEAEKVESGSKYDPW 323

Query: 390 KICPVTQVEEVKCILRLLPVWLCTIFSSVVFIQMISLFVEQGAAMDTLVVSNFHIPPASM 449
           ++C VTQVEEVKC+LRLLP+WLCTI  SVVF QM SLFV QGAAM T  + NF IP +SM
Sbjct: 324 RLCSVTQVEEVKCVLRLLPIWLCTILYSVVFTQMASLFVVQGAAMKT-NIKNFRIPASSM 383

Query: 450 TAFDIVSTTMFIMLYDRLLVPLYVRIAK-RKPKAPNELQRIGIGLAIAIVALVIAGFVEQ 509
           ++FDI+S   FI  Y R L PL+ R+ K  + K   ELQR+GIGL IAI+A++ AG VE 
Sbjct: 384 SSFDILSVAFFIFAYRRFLDPLFARLNKTERNKGLTELQRMGIGLVIAIMAMISAGIVEI 443

Query: 510 KRLKH----AGVSGKEISSFSIFWQTPQYVLVGVAEAFVYVAQMDFFTSQIPEGLKSLGM 569
            RLK+    +  S    S+ SIFWQ PQY+L+G +E F+YV Q++FF SQ P GLKS   
Sbjct: 444 HRLKNKEPESATSISSSSTLSIFWQVPQYMLIGASEVFMYVGQLEFFNSQAPTGLKSFAS 503

Query: 570 GLSMSSTAMGSYVASLILTTVMAITKTNGNPGWVPPNLNDGHLDRFFFLSAALTAFNLAL 629
            L M+S ++G+YV+SL+++ VM I+ T+   GW+P NLN GHL+RF+FL A LTA +  +
Sbjct: 504 ALCMASISLGNYVSSLLVSIVMKISTTDDVHGWIPENLNKGHLERFYFLLAGLTAADFVV 563

Query: 630 YIVCAKQYK 633
           Y++CAK YK
Sbjct: 564 YLICAKWYK 571

BLAST of Cucsa.362690 vs. TAIR10
Match: AT5G19640.1 (AT5G19640.1 Major facilitator superfamily protein)

HSP 1 Score: 530.0 bits (1364), Expect = 2.0e-150
Identity = 274/545 (50.28%), Postives = 367/545 (67.34%), Query Frame = 1

Query: 93  RKVGGWNCGMLLLVNQGLITLAFAGVEVNLVLFSKSVLRQTNAEAANMFSRWMGTTYCFS 152
           +K GGW   ++LLVNQGL TLAF GV VNLVLF   V+ Q NAEAAN  S+W GT Y FS
Sbjct: 58  KKNGGWTNAIILLVNQGLATLAFFGVGVNLVLFLTRVMGQGNAEAANNVSKWTGTVYMFS 117

Query: 153 LLGAFLSDSYLGRYLTCVIFQLVFIVGLIALSLLTHISLLKPNGCGKIGQLCDPHSLTEL 212
           L+GAFLSDSY GRYLTC IFQ++F++G+  LS ++   L+KP GCG     C+P S   +
Sbjct: 118 LVGAFLSDSYWGRYLTCTIFQVIFVIGVGLLSFVSWFFLIKPRGCGDGDLECNPPSSLGV 177

Query: 213 SIFYISIYLIALGNGAPEPALATFGVEQFDDKDPKENRAKTSFYSYFYVSINMGCLIAET 272
           +IFY+S+YL+A G G  +P LATFG +Q DD    +  +K +F+SYFY ++N+G L + T
Sbjct: 178 AIFYLSVYLVAFGYGGHQPTLATFGADQLDD----DKNSKAAFFSYFYFALNVGALFSNT 237

Query: 273 ILVYIENLGHWMVGFWICAVCGLLAYLLFLSGTPRYRQSKPCGNPISRFSQVLMASVKKM 332
           ILVY E+ G W  GF +     ++A + FL+ T +YR  KPCGNP+ R +QV +A+ +K 
Sbjct: 238 ILVYFEDKGLWTEGFLVSLGSAIVALVAFLAPTRQYRYVKPCGNPLPRVAQVFVATARKW 297

Query: 333 NLPMPSNGEGLYEGLGK----KGTRRMLHTEGFKFLDRAAILTAEEANLICNHGQTPNPW 392
           ++  P +   LYE  G     KG+R++ H+  F FLDRAA++T  + N     G   N W
Sbjct: 298 SVVRPGDPHELYELEGPESAIKGSRKIFHSTKFLFLDRAAVITENDRN-----GTRSNAW 357

Query: 393 KICPVTQVEEVKCILRLLPVWLCTIFSSVVFIQMISLFVEQGAAMDTLVVSNFHIPPASM 452
           ++C VTQVEE KC+++LLP+WLCTI  SV+F QM SLFVEQG  M+   V  FHIP ASM
Sbjct: 358 RLCSVTQVEEAKCVMKLLPIWLCTIIYSVIFTQMASLFVEQGDVMNA-YVGKFHIPAASM 417

Query: 453 TAFDIVSTTMFIMLYDRLLVPLYVRIAKRKPKAPNELQRIGIGLAIAIVALVIAGFVEQK 512
           + FDI S  +   +Y  ++ P YVR        P EL R+GIGL I I+A+V AG  E +
Sbjct: 418 SVFDIFSVFVSTGIYRHIIFP-YVR--------PTELMRMGIGLIIGIMAMVAAGLTEIQ 477

Query: 513 RLKHAGVSGKEISSFSIFWQTPQYVLVGVAEAFVYVAQMDFFTSQIPEGLKSLGMGLSMS 572
           RLK   V G++ S  +I WQ PQYVLVG +E F+YV Q++FF  Q P+GLK+LG  L M+
Sbjct: 478 RLKRV-VPGQKESELTILWQIPQYVLVGASEVFMYVGQLEFFNGQAPDGLKNLGSSLCMA 537

Query: 573 STAMGSYVASLILTTVMAITKTNGN-PGWVPPNLNDGHLDRFFFLSAALTAFNLALYIVC 632
           S A+G+YV+SL++  VMAITK   N PGW+P NLN+GH+DRF+FL AAL A +  +Y++ 
Sbjct: 538 SMALGNYVSSLMVNIVMAITKRGENSPGWIPENLNEGHMDRFYFLIAALAAIDFVVYLIF 582

BLAST of Cucsa.362690 vs. TAIR10
Match: AT3G54140.1 (AT3G54140.1 peptide transporter 1)

HSP 1 Score: 434.1 bits (1115), Expect = 1.5e-121
Identity = 224/554 (40.43%), Postives = 347/554 (62.64%), Query Frame = 1

Query: 87  KTTANRRKVGGWNCGMLLLVNQGLITLAFAGVEVNLVLFSKSVLRQTNAEAANMFSRWMG 146
           K  AN+ K G W     +L N+    LA+ G+  NLV + +S L Q NA AAN  + W G
Sbjct: 17  KNPANKEKTGNWKACRFILGNECCERLAYYGMGTNLVNYLESRLNQGNATAANNVTNWSG 76

Query: 147 TTYCFSLLGAFLSDSYLGRYLTCVIFQLVFIVGLIALSLLTHISLLKPNGCGKIGQLCDP 206
           T Y   L+GAF++D+YLGRY T   F  +++ G+  L+L   +  LKP  C      C P
Sbjct: 77  TCYITPLIGAFIADAYLGRYWTIATFVFIYVSGMTLLTLSASVPGLKPGNCN--ADTCHP 136

Query: 207 HSLTELSIFYISIYLIALGNGAPEPALATFGVEQFDDKDPKENRAKTSFYSYFYVSINMG 266
           +S ++ ++F++++Y+IALG G  +P +++FG +QFD+ D  E   K+SF+++FY SIN+G
Sbjct: 137 NS-SQTAVFFVALYMIALGTGGIKPCVSSFGADQFDENDENEKIKKSSFFNWFYFSINVG 196

Query: 267 CLIAETILVYIENLGHWMVGFWICAVCGLLAYLLFLSGTPRYRQSKPCGNPISRFSQVLM 326
            LIA T+LV+I+    W  GF +  V  ++A   F  G+  YR  +P G+P++R  QV++
Sbjct: 197 ALIAATVLVWIQMNVGWGWGFGVPTVAMVIAVCFFFFGSRFYRLQRPGGSPLTRIFQVIV 256

Query: 327 ASVKKMNLPMPSNGEGLYEGLGK----KGTRRMLHTEGFKFLDRAAILTAEEANLICNHG 386
           A+ +K+++ +P +   L+E        KG+R+++HT+  KF D+AA+ +  ++      G
Sbjct: 257 AAFRKISVKVPEDKSLLFETADDESNIKGSRKLVHTDNLKFFDKAAVESQSDS---IKDG 316

Query: 387 QTPNPWKICPVTQVEEVKCILRLLPVWLCTIFSSVVFIQMISLFVEQGAAMDTLVVSNFH 446
           +  NPW++C VTQVEE+K I+ LLPVW   I  + V+ QM ++FV QG  MD  +  NF 
Sbjct: 317 EV-NPWRLCSVTQVEELKSIITLLPVWATGIVFATVYSQMSTMFVLQGNTMDQHMGKNFE 376

Query: 447 IPPASMTAFDIVSTTMFIMLYDRLLVPLYVRIAKRKPKAPNELQRIGIGLAIAIVALVIA 506
           IP AS++ FD VS   +  +YD+ ++PL  R   R  +   +LQR+GIGL ++I A++ A
Sbjct: 377 IPSASLSLFDTVSVLFWTPVYDQFIIPL-ARKFTRNERGFTQLQRMGIGLVVSIFAMITA 436

Query: 507 GFVEQKRLK----HAGVSGKEISSFSIFWQTPQYVLVGVAEAFVYVAQMDFFTSQIPEGL 566
           G +E  RL     H     K+I   SIFWQ PQY+L+G AE F ++ Q++FF  Q P+ +
Sbjct: 437 GVLEVVRLDYVKTHNAYDQKQIH-MSIFWQIPQYLLIGCAEVFTFIGQLEFFYDQAPDAM 496

Query: 567 KSLGMGLSMSSTAMGSYVASLILTTVMAITKTNGNPGWVPPNLNDGHLDRFFFLSAALTA 626
           +SL   LS+++ A+G+Y++++++T VM ITK NG PGW+P NLN GHLD FF+L A L+ 
Sbjct: 497 RSLCSALSLTTVALGNYLSTVLVTVVMKITKKNGKPGWIPDNLNRGHLDYFFYLLATLSF 556

Query: 627 FNLALYIVCAKQYK 633
            N  +Y+  +K+YK
Sbjct: 557 LNFLVYLWISKRYK 561

BLAST of Cucsa.362690 vs. TAIR10
Match: AT1G62200.1 (AT1G62200.1 Major facilitator superfamily protein)

HSP 1 Score: 431.4 bits (1108), Expect = 9.8e-121
Identity = 226/566 (39.93%), Postives = 351/566 (62.01%), Query Frame = 1

Query: 73  SSSLEDGALTDQNDKTTANRRKVGGWNCGMLLLVNQGLITLAFAGVEVNLVLFSKSVLRQ 132
           SS+ EDG++    +  +  ++K G W     +L N+    LA+ G+  NL+ +  S L +
Sbjct: 33  SSTAEDGSIDIYGNPPS--KKKTGNWKACPFILGNECCERLAYYGIAKNLITYYTSELHE 92

Query: 133 TNAEAANMFSRWMGTTYCFSLLGAFLSDSYLGRYLTCVIFQLVFIVGLIALSLLTHISLL 192
           +N  AA+    W GT Y   L+GA ++DSY GRY T   F  ++ +G+  L+L   + +L
Sbjct: 93  SNVSAASDVMIWQGTCYITPLIGAVIADSYWGRYWTIASFSAIYFIGMALLTLSASLPVL 152

Query: 193 KPNGC-GKIGQLCDPHSLTELSIFYISIYLIALGNGAPEPALATFGVEQFDDKDPKENRA 252
           KP  C G    LC P +  + ++F+  +YLIALG G  +P +++FG +QFDD DP+E   
Sbjct: 153 KPAACAGVAAALCSPATTVQYAVFFTGLYLIALGTGGIKPCVSSFGADQFDDTDPRERVR 212

Query: 253 KTSFYSYFYVSINMGCLIAETILVYIENLGHWMVGFWICAVCGLLAYLLFLSGTPRYRQS 312
           K SF+++FY SIN+G  I+ T+LV+++    W +GF I  V   ++   F  GTP YR  
Sbjct: 213 KASFFNWFYFSINIGSFISSTLLVWVQENVGWGLGFLIPTVFMGVSIASFFIGTPLYRFQ 272

Query: 313 KPCGNPISRFSQVLMASVKKMNLPMPSNGEGLYEGLGKK----GTRRMLHTEGFKFLDRA 372
           KP G+PI+R  QVL+A+ +K+ L +P +   LYE   K     G+R++ HT+G+KFLD+A
Sbjct: 273 KPGGSPITRVCQVLVAAYRKLKLNLPEDISFLYETREKNSMIAGSRKIQHTDGYKFLDKA 332

Query: 373 AILTAEEANLICNHGQTPNPWKICPVTQVEEVKCILRLLPVWLCTIFSSVVFIQMISLFV 432
           A+++  E+      G   NPWK+C VTQVEEVK ++R+ P+W   I  SV++ Q+ +LFV
Sbjct: 333 AVISEYES----KSGAFSNPWKLCTVTQVEEVKTLIRMFPIWASGIVYSVLYSQISTLFV 392

Query: 433 EQGAAMDTLVVSNFHIPPASMTAFDIVSTTMFIMLYDRLLVPLYVRIAKRKPKAPNELQR 492
           +QG +M+  ++ +F IPPAS   FD +   + I +YDR LVP +VR     PK   +LQR
Sbjct: 393 QQGRSMNR-IIRSFEIPPASFGVFDTLIVLISIPIYDRFLVP-FVRRFTGIPKGLTDLQR 452

Query: 493 IGIGLAIAIVALVIAGFVEQKRLKHAGVSGKEISSFSIFWQTPQYVLVGVAEAFVYVAQM 552
           +GIGL ++++++  A  VE  RL+ A    ++  + SIFWQ PQY+L+G+AE F ++ ++
Sbjct: 453 MGIGLFLSVLSIAAAAIVETVRLQLA----QDFVAMSIFWQIPQYILMGIAEVFFFIGRV 512

Query: 553 DFFTSQIPEGLKSLGMGLSMSSTAMGSYVASLILTTVMAITKTNGNPGWVPPNLNDGHLD 612
           +FF  + P+ ++S+   L++ +TA+GSY++SLILT V   T   G  GWVP +LN GHLD
Sbjct: 513 EFFYDESPDAMRSVCSALALLNTAVGSYLSSLILTLVAYFTALGGKDGWVPDDLNKGHLD 572

Query: 613 RFFFLSAALTAFNLALY-IVCAKQYK 633
            FF+L  +L   N+ +Y ++C K  K
Sbjct: 573 YFFWLLVSLGLVNIPVYALICVKHTK 586

BLAST of Cucsa.362690 vs. NCBI nr
Match: gi|778711234|ref|XP_011656704.1| (PREDICTED: protein NRT1/ PTR FAMILY 7.3-like [Cucumis sativus])

HSP 1 Score: 1307.4 bits (3382), Expect = 0.0e+00
Identity = 652/652 (100.00%), Postives = 652/652 (100.00%), Query Frame = 1

Query: 1   MHGQLYFQFYSISSPSLITLNSNLRFPSNFSINQQWNHFFFRAMESKPDLKSNDELQVAD 60
           MHGQLYFQFYSISSPSLITLNSNLRFPSNFSINQQWNHFFFRAMESKPDLKSNDELQVAD
Sbjct: 1   MHGQLYFQFYSISSPSLITLNSNLRFPSNFSINQQWNHFFFRAMESKPDLKSNDELQVAD 60

Query: 61  LHLLENGQERFQSSSLEDGALTDQNDKTTANRRKVGGWNCGMLLLVNQGLITLAFAGVEV 120
           LHLLENGQERFQSSSLEDGALTDQNDKTTANRRKVGGWNCGMLLLVNQGLITLAFAGVEV
Sbjct: 61  LHLLENGQERFQSSSLEDGALTDQNDKTTANRRKVGGWNCGMLLLVNQGLITLAFAGVEV 120

Query: 121 NLVLFSKSVLRQTNAEAANMFSRWMGTTYCFSLLGAFLSDSYLGRYLTCVIFQLVFIVGL 180
           NLVLFSKSVLRQTNAEAANMFSRWMGTTYCFSLLGAFLSDSYLGRYLTCVIFQLVFIVGL
Sbjct: 121 NLVLFSKSVLRQTNAEAANMFSRWMGTTYCFSLLGAFLSDSYLGRYLTCVIFQLVFIVGL 180

Query: 181 IALSLLTHISLLKPNGCGKIGQLCDPHSLTELSIFYISIYLIALGNGAPEPALATFGVEQ 240
           IALSLLTHISLLKPNGCGKIGQLCDPHSLTELSIFYISIYLIALGNGAPEPALATFGVEQ
Sbjct: 181 IALSLLTHISLLKPNGCGKIGQLCDPHSLTELSIFYISIYLIALGNGAPEPALATFGVEQ 240

Query: 241 FDDKDPKENRAKTSFYSYFYVSINMGCLIAETILVYIENLGHWMVGFWICAVCGLLAYLL 300
           FDDKDPKENRAKTSFYSYFYVSINMGCLIAETILVYIENLGHWMVGFWICAVCGLLAYLL
Sbjct: 241 FDDKDPKENRAKTSFYSYFYVSINMGCLIAETILVYIENLGHWMVGFWICAVCGLLAYLL 300

Query: 301 FLSGTPRYRQSKPCGNPISRFSQVLMASVKKMNLPMPSNGEGLYEGLGKKGTRRMLHTEG 360
           FLSGTPRYRQSKPCGNPISRFSQVLMASVKKMNLPMPSNGEGLYEGLGKKGTRRMLHTEG
Sbjct: 301 FLSGTPRYRQSKPCGNPISRFSQVLMASVKKMNLPMPSNGEGLYEGLGKKGTRRMLHTEG 360

Query: 361 FKFLDRAAILTAEEANLICNHGQTPNPWKICPVTQVEEVKCILRLLPVWLCTIFSSVVFI 420
           FKFLDRAAILTAEEANLICNHGQTPNPWKICPVTQVEEVKCILRLLPVWLCTIFSSVVFI
Sbjct: 361 FKFLDRAAILTAEEANLICNHGQTPNPWKICPVTQVEEVKCILRLLPVWLCTIFSSVVFI 420

Query: 421 QMISLFVEQGAAMDTLVVSNFHIPPASMTAFDIVSTTMFIMLYDRLLVPLYVRIAKRKPK 480
           QMISLFVEQGAAMDTLVVSNFHIPPASMTAFDIVSTTMFIMLYDRLLVPLYVRIAKRKPK
Sbjct: 421 QMISLFVEQGAAMDTLVVSNFHIPPASMTAFDIVSTTMFIMLYDRLLVPLYVRIAKRKPK 480

Query: 481 APNELQRIGIGLAIAIVALVIAGFVEQKRLKHAGVSGKEISSFSIFWQTPQYVLVGVAEA 540
           APNELQRIGIGLAIAIVALVIAGFVEQKRLKHAGVSGKEISSFSIFWQTPQYVLVGVAEA
Sbjct: 481 APNELQRIGIGLAIAIVALVIAGFVEQKRLKHAGVSGKEISSFSIFWQTPQYVLVGVAEA 540

Query: 541 FVYVAQMDFFTSQIPEGLKSLGMGLSMSSTAMGSYVASLILTTVMAITKTNGNPGWVPPN 600
           FVYVAQMDFFTSQIPEGLKSLGMGLSMSSTAMGSYVASLILTTVMAITKTNGNPGWVPPN
Sbjct: 541 FVYVAQMDFFTSQIPEGLKSLGMGLSMSSTAMGSYVASLILTTVMAITKTNGNPGWVPPN 600

Query: 601 LNDGHLDRFFFLSAALTAFNLALYIVCAKQYKGSIIEKQDGDQEEEPDREFK 653
           LNDGHLDRFFFLSAALTAFNLALYIVCAKQYKGSIIEKQDGDQEEEPDREFK
Sbjct: 601 LNDGHLDRFFFLSAALTAFNLALYIVCAKQYKGSIIEKQDGDQEEEPDREFK 652

BLAST of Cucsa.362690 vs. NCBI nr
Match: gi|700191047|gb|KGN46251.1| (hypothetical protein Csa_6G077970 [Cucumis sativus])

HSP 1 Score: 1190.6 bits (3079), Expect = 0.0e+00
Identity = 595/596 (99.83%), Postives = 596/596 (100.00%), Query Frame = 1

Query: 57  QVADLHLLENGQERFQSSSLEDGALTDQNDKTTANRRKVGGWNCGMLLLVNQGLITLAFA 116
           +VADLHLLENGQERFQSSSLEDGALTDQNDKTTANRRKVGGWNCGMLLLVNQGLITLAFA
Sbjct: 4   KVADLHLLENGQERFQSSSLEDGALTDQNDKTTANRRKVGGWNCGMLLLVNQGLITLAFA 63

Query: 117 GVEVNLVLFSKSVLRQTNAEAANMFSRWMGTTYCFSLLGAFLSDSYLGRYLTCVIFQLVF 176
           GVEVNLVLFSKSVLRQTNAEAANMFSRWMGTTYCFSLLGAFLSDSYLGRYLTCVIFQLVF
Sbjct: 64  GVEVNLVLFSKSVLRQTNAEAANMFSRWMGTTYCFSLLGAFLSDSYLGRYLTCVIFQLVF 123

Query: 177 IVGLIALSLLTHISLLKPNGCGKIGQLCDPHSLTELSIFYISIYLIALGNGAPEPALATF 236
           IVGLIALSLLTHISLLKPNGCGKIGQLCDPHSLTELSIFYISIYLIALGNGAPEPALATF
Sbjct: 124 IVGLIALSLLTHISLLKPNGCGKIGQLCDPHSLTELSIFYISIYLIALGNGAPEPALATF 183

Query: 237 GVEQFDDKDPKENRAKTSFYSYFYVSINMGCLIAETILVYIENLGHWMVGFWICAVCGLL 296
           GVEQFDDKDPKENRAKTSFYSYFYVSINMGCLIAETILVYIENLGHWMVGFWICAVCGLL
Sbjct: 184 GVEQFDDKDPKENRAKTSFYSYFYVSINMGCLIAETILVYIENLGHWMVGFWICAVCGLL 243

Query: 297 AYLLFLSGTPRYRQSKPCGNPISRFSQVLMASVKKMNLPMPSNGEGLYEGLGKKGTRRML 356
           AYLLFLSGTPRYRQSKPCGNPISRFSQVLMASVKKMNLPMPSNGEGLYEGLGKKGTRRML
Sbjct: 244 AYLLFLSGTPRYRQSKPCGNPISRFSQVLMASVKKMNLPMPSNGEGLYEGLGKKGTRRML 303

Query: 357 HTEGFKFLDRAAILTAEEANLICNHGQTPNPWKICPVTQVEEVKCILRLLPVWLCTIFSS 416
           HTEGFKFLDRAAILTAEEANLICNHGQTPNPWKICPVTQVEEVKCILRLLPVWLCTIFSS
Sbjct: 304 HTEGFKFLDRAAILTAEEANLICNHGQTPNPWKICPVTQVEEVKCILRLLPVWLCTIFSS 363

Query: 417 VVFIQMISLFVEQGAAMDTLVVSNFHIPPASMTAFDIVSTTMFIMLYDRLLVPLYVRIAK 476
           VVFIQMISLFVEQGAAMDTLVVSNFHIPPASMTAFDIVSTTMFIMLYDRLLVPLYVRIAK
Sbjct: 364 VVFIQMISLFVEQGAAMDTLVVSNFHIPPASMTAFDIVSTTMFIMLYDRLLVPLYVRIAK 423

Query: 477 RKPKAPNELQRIGIGLAIAIVALVIAGFVEQKRLKHAGVSGKEISSFSIFWQTPQYVLVG 536
           RKPKAPNELQRIGIGLAIAIVALVIAGFVEQKRLKHAGVSGKEISSFSIFWQTPQYVLVG
Sbjct: 424 RKPKAPNELQRIGIGLAIAIVALVIAGFVEQKRLKHAGVSGKEISSFSIFWQTPQYVLVG 483

Query: 537 VAEAFVYVAQMDFFTSQIPEGLKSLGMGLSMSSTAMGSYVASLILTTVMAITKTNGNPGW 596
           VAEAFVYVAQMDFFTSQIPEGLKSLGMGLSMSSTAMGSYVASLILTTVMAITKTNGNPGW
Sbjct: 484 VAEAFVYVAQMDFFTSQIPEGLKSLGMGLSMSSTAMGSYVASLILTTVMAITKTNGNPGW 543

Query: 597 VPPNLNDGHLDRFFFLSAALTAFNLALYIVCAKQYKGSIIEKQDGDQEEEPDREFK 653
           VPPNLNDGHLDRFFFLSAALTAFNLALYIVCAKQYKGSIIEKQDGDQEEEPDREFK
Sbjct: 544 VPPNLNDGHLDRFFFLSAALTAFNLALYIVCAKQYKGSIIEKQDGDQEEEPDREFK 599

BLAST of Cucsa.362690 vs. NCBI nr
Match: gi|659120269|ref|XP_008460104.1| (PREDICTED: protein NRT1/ PTR FAMILY 7.3-like isoform X1 [Cucumis melo])

HSP 1 Score: 1177.5 bits (3045), Expect = 0.0e+00
Identity = 589/613 (96.08%), Postives = 597/613 (97.39%), Query Frame = 1

Query: 40  FFRAMESKPDLKSNDELQVADLHLLENGQERFQSSSLEDGALTDQNDKTTANRRKVGGWN 99
           FFRAMESKPDLKSNDELQVADL LLENGQERFQ SS EDGAL DQN+KT A RRKVGGWN
Sbjct: 12  FFRAMESKPDLKSNDELQVADLQLLENGQERFQRSSSEDGALADQNEKTIAKRRKVGGWN 71

Query: 100 CGMLLLVNQGLITLAFAGVEVNLVLFSKSVLRQTNAEAANMFSRWMGTTYCFSLLGAFLS 159
           CGMLLLVNQGLITLAFAGVEVNLVLFSKSVLRQTNAEAAN FSRWMGTTYCFSLLGAFLS
Sbjct: 72  CGMLLLVNQGLITLAFAGVEVNLVLFSKSVLRQTNAEAANTFSRWMGTTYCFSLLGAFLS 131

Query: 160 DSYLGRYLTCVIFQLVFIVGLIALSLLTHISLLKPNGCGKIGQLCDPHSLTELSIFYISI 219
           DSYLGRYLTC IFQLVFIVGLI+LSLLTHISLLKPNGCGKIGQLCDPHSLTELSIFYISI
Sbjct: 132 DSYLGRYLTCAIFQLVFIVGLISLSLLTHISLLKPNGCGKIGQLCDPHSLTELSIFYISI 191

Query: 220 YLIALGNGAPEPALATFGVEQFDDKDPKENRAKTSFYSYFYVSINMGCLIAETILVYIEN 279
           YL+ALGNGAPEPALATFG EQFDDKDPKENRAKTSFYSYFYVSINMGCLIAETILVYIEN
Sbjct: 192 YLLALGNGAPEPALATFGAEQFDDKDPKENRAKTSFYSYFYVSINMGCLIAETILVYIEN 251

Query: 280 LGHWMVGFWICAVCGLLAYLLFLSGTPRYRQSKPCGNPISRFSQVLMASVKKMNLPMPSN 339
           LGHW VGFWICAVCGLLAYLLFLSGT RYRQSKPCGNPISRFSQVLMASVKKMNLPMPSN
Sbjct: 252 LGHWKVGFWICAVCGLLAYLLFLSGTSRYRQSKPCGNPISRFSQVLMASVKKMNLPMPSN 311

Query: 340 GEGLYEGLGKKGTRRMLHTEGFKFLDRAAILTAEEANLICNHGQTPNPWKICPVTQVEEV 399
           GEGLYEGLGK+GTRRMLHTEGFKFLDRAAILTAEEANLICN  QTPNPW++CPVTQVEEV
Sbjct: 312 GEGLYEGLGKEGTRRMLHTEGFKFLDRAAILTAEEANLICNQAQTPNPWQLCPVTQVEEV 371

Query: 400 KCILRLLPVWLCTIFSSVVFIQMISLFVEQGAAMDTLVVSNFHIPPASMTAFDIVSTTMF 459
           KCILRLLPVWLCTIFSSVVFIQMISLFVEQGAAMDTLVVSNFHIPPASMTAFDIVSTTMF
Sbjct: 372 KCILRLLPVWLCTIFSSVVFIQMISLFVEQGAAMDTLVVSNFHIPPASMTAFDIVSTTMF 431

Query: 460 IMLYDRLLVPLYVRIAKRKPKAPNELQRIGIGLAIAIVALVIAGFVEQKRLKHAGVSGKE 519
           IMLYDRLLVPLYVRIAKRKPK PNELQRIGIGLAIAIVALVIAGFVEQKRLKHAGVSGKE
Sbjct: 432 IMLYDRLLVPLYVRIAKRKPKPPNELQRIGIGLAIAIVALVIAGFVEQKRLKHAGVSGKE 491

Query: 520 ISSFSIFWQTPQYVLVGVAEAFVYVAQMDFFTSQIPEGLKSLGMGLSMSSTAMGSYVASL 579
           ISS SIFWQTPQYVLVGVAEAFV+VAQMDFFTSQIPEGLKSLGMGLSMSSTAMGSYVASL
Sbjct: 492 ISSLSIFWQTPQYVLVGVAEAFVFVAQMDFFTSQIPEGLKSLGMGLSMSSTAMGSYVASL 551

Query: 580 ILTTVMAITKTNGNPGWVPPNLNDGHLDRFFFLSAALTAFNLALYIVCAKQYKGSIIEKQ 639
           ILTTVMAITKTNG+PGWVPPNLNDGHLDRFFFLSAALTAFNLALYIVCAKQYKGSIIEKQ
Sbjct: 552 ILTTVMAITKTNGHPGWVPPNLNDGHLDRFFFLSAALTAFNLALYIVCAKQYKGSIIEKQ 611

Query: 640 DGDQEEEPDREFK 653
           DGDQEEE DREFK
Sbjct: 612 DGDQEEESDREFK 624

BLAST of Cucsa.362690 vs. NCBI nr
Match: gi|659120271|ref|XP_008460105.1| (PREDICTED: protein NRT1/ PTR FAMILY 7.3-like isoform X2 [Cucumis melo])

HSP 1 Score: 1142.5 bits (2954), Expect = 0.0e+00
Identity = 571/596 (95.81%), Postives = 580/596 (97.32%), Query Frame = 1

Query: 57  QVADLHLLENGQERFQSSSLEDGALTDQNDKTTANRRKVGGWNCGMLLLVNQGLITLAFA 116
           +VADL LLENGQERFQ SS EDGAL DQN+KT A RRKVGGWNCGMLLLVNQGLITLAFA
Sbjct: 10  KVADLQLLENGQERFQRSSSEDGALADQNEKTIAKRRKVGGWNCGMLLLVNQGLITLAFA 69

Query: 117 GVEVNLVLFSKSVLRQTNAEAANMFSRWMGTTYCFSLLGAFLSDSYLGRYLTCVIFQLVF 176
           GVEVNLVLFSKSVLRQTNAEAAN FSRWMGTTYCFSLLGAFLSDSYLGRYLTC IFQLVF
Sbjct: 70  GVEVNLVLFSKSVLRQTNAEAANTFSRWMGTTYCFSLLGAFLSDSYLGRYLTCAIFQLVF 129

Query: 177 IVGLIALSLLTHISLLKPNGCGKIGQLCDPHSLTELSIFYISIYLIALGNGAPEPALATF 236
           IVGLI+LSLLTHISLLKPNGCGKIGQLCDPHSLTELSIFYISIYL+ALGNGAPEPALATF
Sbjct: 130 IVGLISLSLLTHISLLKPNGCGKIGQLCDPHSLTELSIFYISIYLLALGNGAPEPALATF 189

Query: 237 GVEQFDDKDPKENRAKTSFYSYFYVSINMGCLIAETILVYIENLGHWMVGFWICAVCGLL 296
           G EQFDDKDPKENRAKTSFYSYFYVSINMGCLIAETILVYIENLGHW VGFWICAVCGLL
Sbjct: 190 GAEQFDDKDPKENRAKTSFYSYFYVSINMGCLIAETILVYIENLGHWKVGFWICAVCGLL 249

Query: 297 AYLLFLSGTPRYRQSKPCGNPISRFSQVLMASVKKMNLPMPSNGEGLYEGLGKKGTRRML 356
           AYLLFLSGT RYRQSKPCGNPISRFSQVLMASVKKMNLPMPSNGEGLYEGLGK+GTRRML
Sbjct: 250 AYLLFLSGTSRYRQSKPCGNPISRFSQVLMASVKKMNLPMPSNGEGLYEGLGKEGTRRML 309

Query: 357 HTEGFKFLDRAAILTAEEANLICNHGQTPNPWKICPVTQVEEVKCILRLLPVWLCTIFSS 416
           HTEGFKFLDRAAILTAEEANLICN  QTPNPW++CPVTQVEEVKCILRLLPVWLCTIFSS
Sbjct: 310 HTEGFKFLDRAAILTAEEANLICNQAQTPNPWQLCPVTQVEEVKCILRLLPVWLCTIFSS 369

Query: 417 VVFIQMISLFVEQGAAMDTLVVSNFHIPPASMTAFDIVSTTMFIMLYDRLLVPLYVRIAK 476
           VVFIQMISLFVEQGAAMDTLVVSNFHIPPASMTAFDIVSTTMFIMLYDRLLVPLYVRIAK
Sbjct: 370 VVFIQMISLFVEQGAAMDTLVVSNFHIPPASMTAFDIVSTTMFIMLYDRLLVPLYVRIAK 429

Query: 477 RKPKAPNELQRIGIGLAIAIVALVIAGFVEQKRLKHAGVSGKEISSFSIFWQTPQYVLVG 536
           RKPK PNELQRIGIGLAIAIVALVIAGFVEQKRLKHAGVSGKEISS SIFWQTPQYVLVG
Sbjct: 430 RKPKPPNELQRIGIGLAIAIVALVIAGFVEQKRLKHAGVSGKEISSLSIFWQTPQYVLVG 489

Query: 537 VAEAFVYVAQMDFFTSQIPEGLKSLGMGLSMSSTAMGSYVASLILTTVMAITKTNGNPGW 596
           VAEAFV+VAQMDFFTSQIPEGLKSLGMGLSMSSTAMGSYVASLILTTVMAITKTNG+PGW
Sbjct: 490 VAEAFVFVAQMDFFTSQIPEGLKSLGMGLSMSSTAMGSYVASLILTTVMAITKTNGHPGW 549

Query: 597 VPPNLNDGHLDRFFFLSAALTAFNLALYIVCAKQYKGSIIEKQDGDQEEEPDREFK 653
           VPPNLNDGHLDRFFFLSAALTAFNLALYIVCAKQYKGSIIEKQDGDQEEE DREFK
Sbjct: 550 VPPNLNDGHLDRFFFLSAALTAFNLALYIVCAKQYKGSIIEKQDGDQEEESDREFK 605

BLAST of Cucsa.362690 vs. NCBI nr
Match: gi|703108148|ref|XP_010098931.1| (Nitrate transporter 1.5 [Morus notabilis])

HSP 1 Score: 827.8 bits (2137), Expect = 1.3e-236
Identity = 409/610 (67.05%), Postives = 502/610 (82.30%), Query Frame = 1

Query: 48  PDLKSN------DELQVADLHLLENGQERFQSSSLEDGALTDQNDKTTANRRKVGGWNCG 107
           P+  SN      +  ++ +L+ LENGQER +++  +DG+L D++ K  A + + GGW CG
Sbjct: 3   PEASSNGTSVLCNNFKMVELNQLENGQERLENACTKDGSL-DRHGKP-ALKGRTGGWRCG 62

Query: 108 MLLLVNQGLITLAFAGVEVNLVLFSKSVLRQTNAEAANMFSRWMGTTYCFSLLGAFLSDS 167
           MLLLVNQGLITLAFAGVEVNLVLFSKSVLR +NAEAA+ FS WMGT Y FSL+GAFLSDS
Sbjct: 63  MLLLVNQGLITLAFAGVEVNLVLFSKSVLRHSNAEAAHTFSTWMGTVYLFSLMGAFLSDS 122

Query: 168 YLGRYLTCVIFQLVFIVGLIALSLLTHISLLKPNGCGKIGQLCDPHSLTELSIFYISIYL 227
           YLGRY+TCVIFQ+V+I+GLI LS+  ++ L++P+GCGKIG++C+PH   E +IFY+SIYL
Sbjct: 123 YLGRYITCVIFQVVYIIGLIVLSMSANLFLIRPHGCGKIGEVCEPHKPLESAIFYMSIYL 182

Query: 228 IALGNGAPEPALATFGVEQFDDKDPKENRAKTSFYSYFYVSINMGCLIAETILVYIENLG 287
           IALGNGA EPALATFG +QFD++D KE R+K SFYSYFYV++N+G L+AETILVYIEN+G
Sbjct: 183 IALGNGASEPALATFGADQFDEEDQKEKRSKASFYSYFYVALNLGSLVAETILVYIENMG 242

Query: 288 HWMVGFWICAVCGLLAYLLFLSGTPRYRQSKPCGNPISRFSQVLMASVKKMNLPMPSNGE 347
           +W+V FW C +  ++ Y+  LSGT RYR  KP GNPISRFSQV++AS++K++  +PSN E
Sbjct: 243 YWVVAFWTCTISAVVGYVFLLSGTLRYRHFKPSGNPISRFSQVIVASMRKLSQNLPSNEE 302

Query: 348 GLYEGLGKK----GTRRMLHTEGFKFLDRAAILTAEEANLICNHGQTPNPWKICPVTQVE 407
            LYE  GK+    G RR+LHT+GFKFLDRAAI+  E+  LI N  +TPNPW +C VTQVE
Sbjct: 303 ELYEVHGKEGETNGMRRILHTQGFKFLDRAAIIMPEDMKLIRNEARTPNPWHLCTVTQVE 362

Query: 408 EVKCILRLLPVWLCTIFSSVVFIQMISLFVEQGAAMDTLVVSNFHIPPASMTAFDIVSTT 467
           EVKC+LRLLPVWLCTIFSSVVFIQM+SLFVEQGAAM+   VSNFHIPPASMT FDIVST+
Sbjct: 363 EVKCVLRLLPVWLCTIFSSVVFIQMLSLFVEQGAAMNR-TVSNFHIPPASMTVFDIVSTS 422

Query: 468 MFIMLYDRLLVPLYVRIAKRKPKAPNELQRIGIGLAIAIVALVIAGFVEQKRLKH-AGVS 527
           +FIMLYD+++VPLY+++ KR+PK P+ LQRIGIGL IA++A++IAG VEQ+RL++     
Sbjct: 423 VFIMLYDKIIVPLYIKLTKREPKIPSPLQRIGIGLTIAVLAMIIAGIVEQQRLRYTTNFV 482

Query: 528 GKEISSFSIFWQTPQYVLVGVAEAFVYVAQMDFFTSQIPEGLKSLGMGLSMSSTAMGSYV 587
            KEISS SI WQTPQYVLVGV+EAFVYVAQM+FF SQ P+GLKSLG+GLSMSS+AMGSYV
Sbjct: 483 RKEISSLSILWQTPQYVLVGVSEAFVYVAQMEFFASQTPDGLKSLGIGLSMSSSAMGSYV 542

Query: 588 ASLILTTVMAITKTNGNPGWVPPNLNDGHLDRFFFLSAALTAFNLALYIVCAKQYKGSII 647
           AS ILT VM IT  NG PGWVPPNLNDGHLDRFFF+SAAL A N  LY+VCAK+YK   +
Sbjct: 543 ASTILTIVMKITSKNGKPGWVPPNLNDGHLDRFFFVSAALAALNFLLYVVCAKRYKSISL 602