Cucsa.350520 (gene) Cucumber (Gy14) v1

Overview
NameCucsa.350520
Typegene
OrganismCucumis sativus (Cucumber (Gy14) v1)
Description2-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily protein
Locationscaffold03504 : 11632 .. 12940 (+)
Genome Viewer
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: five_prime_UTRCDS
Hold the cursor over a type above to highlight its positions in the sequence below.
ACTGTTCAAGATGTAGCTTCTAAAGGGGAAGTCCCTGAAAGATACATCCATAAAGAAAGCGATCGAGGAGCTCGAAATGCTCCGCTAATGGCAGCTCCGGTAATCGACATCGCTCTCCTCTCCTCTTCATCCAAATCTGGACCGGAACTGGAGAAACTCCGACATGGACTTCAATCATGGGGCTGCTTTCAGGTTCGATTTCTCTTGATTTTCCACTCTAGAACACTAAATCGTAAAGGGGGTTAGGAATTTCTGATTTCTTTGGACTCTGTATCTCGATCCCATTAAAAaTTAAATTGAAGATATTCATAAATGTTTGTGATGTTCTTATAGGCTATAAATCATGGAATGACAAGTGAATATCTGGATGAAGTTCGTCGACTAACGAAACAATTCTTTGGTCTTTCAATGGAAGAGAAATTAAAATACTTGAAAGAAGAACTTGAAATGGAAGGATATGGGAACGACATGATTCTTTCAAACCAACAAATTCTTGATTGGACTGATCGTTTATACCTTACTGTATATCCACACCAAAGCCGTCGTTTCAAGTATTGGCCAACAAaTCTTCAAAGATTTAGGTTACTTTTTCTTCCTCAAATCTTGTTTTGTTTGTGATTTTGTTTATGAATCTACATAAACATATCTTGATCTGGAATTCATTGTAGGGAAGTTATTGATGAGTACACTACTAATGTGAAGCTGATAAGTGAGAAAATCTTCAAGGCCATGGCAAGATCATTGGATTTAGATGAGAGTAGTTTTCTTAAGCAATACGGCGAGCAAATTAAACTCGACGCACGGTTCAACTTCTACCCTCGATGTCGAAATCCCGATCTTGTTCTCGGTGTTAAGCCGCACGCCGATGGATCGGCAATCACCATTTTGTTGCAGGATAAGGAAGTAGAAGGTCTTCAGTTCATGAAAGATAATGAGTGGTTCAATGCTCCGATTGTTCCGGACGCTCTTCTCGTCAATGTGGGGGATCAAGTAGAGGTAAAAGAAGAAACACTTGTTTATTCTAAAATCACTGTGAAACATGTTGGTAAAGAGAGTGGTGATTTGATTGTAGATCACAAGTAATGGGATATTCAAGAGTCCAGTTCATAGAGTATTGACAAATTCAGAGAGGGAGAGGATATCGTTGGCAGTGTTTTACCTGCCAGACGCAGAAAAAGAAATTGAACCATTAGAGGAGCTGATTAGTGAAACTCAGCCAAGGTTGTATAAGAGTGTGAAGAACTTTGTTGGGCTTTACTTTGAATACTACCAACAGGGACAGAGACCAATGGAGGCTGCAAGAATCTAA

mRNA sequence

ACTGTTCAAGATGTAGCTTCTAAAGGGGAAGTCCCTGAAAGATACATCCATAAAGAAAGCGATCGAGGAGCTCGAAATGCTCCGCTAATGGCAGCTCCGGTAATCGACATCGCTCTCCTCTCCTCTTCATCCAAATCTGGACCGGAACTGGAGAAACTCCGACATGGACTTCAATCATGGGGCTGCTTTCAGGCTATAAATCATGGAATGACAAGTGAATATCTGGATGAAGTTCGTCGACTAACGAAACAATTCTTTGGTCTTTCAATGGAAGAGAAATTAAAATACTTGAAAGAAGAACTTGAAATGGAAGGATATGGGAACGACATGATTCTTTCAAACCAACAAATTCTTGATTGGACTGATCGTTTATACCTTACTGTATATCCACACCAAAGCCGTCGTTTCAAGTATTGGCCAACAAATCTTCAAAGATTTAGGGAAGTTATTGATGAGTACACTACTAATGTGAAGCTGATAAGTGAGAAAATCTTCAAGGCCATGGCAAGATCATTGGATTTAGATGAGAGTAGTTTTCTTAAGCAATACGGCGAGCAAATTAAACTCGACGCACGGTTCAACTTCTACCCTCGATGTCGAAATCCCGATCTTGTTCTCGGTGTTAAGCCGCACGCCGATGGATCGGCAATCACCATTTTGTTGCAGGATAAGGAAGTAGAAGGTCTTCAGTTCATGAAAGATAATGAGTGGTTCAATGCTCCGATTGTTCCGGACGCTCTTCTCGTCAATGTGGGGGATCAAGTAGAGATCACAAGTAATGGGATATTCAAGAGTCCAGTTCATAGAGTATTGACAAATTCAGAGAGGGAGAGGATATCGTTGGCAGTGTTTTACCTGCCAGACGCAGAAAAAGAAATTGAACCATTAGAGGAGCTGATTAGTGAAACTCAGCCAAGGTTGTATAAGAGTGTGAAGAACTTTGTTGGGCTTTACTTTGAATACTACCAACAGGGACAGAGACCAATGGAGGCTGCAAGAATCTAA

Coding sequence (CDS)

ATGGCAGCTCCGGTAATCGACATCGCTCTCCTCTCCTCTTCATCCAAATCTGGACCGGAACTGGAGAAACTCCGACATGGACTTCAATCATGGGGCTGCTTTCAGGCTATAAATCATGGAATGACAAGTGAATATCTGGATGAAGTTCGTCGACTAACGAAACAATTCTTTGGTCTTTCAATGGAAGAGAAATTAAAATACTTGAAAGAAGAACTTGAAATGGAAGGATATGGGAACGACATGATTCTTTCAAACCAACAAATTCTTGATTGGACTGATCGTTTATACCTTACTGTATATCCACACCAAAGCCGTCGTTTCAAGTATTGGCCAACAAaTCTTCAAAGATTTAGGGAAGTTATTGATGAGTACACTACTAATGTGAAGCTGATAAGTGAGAAAATCTTCAAGGCCATGGCAAGATCATTGGATTTAGATGAGAGTAGTTTTCTTAAGCAATACGGCGAGCAAATTAAACTCGACGCACGGTTCAACTTCTACCCTCGATGTCGAAATCCCGATCTTGTTCTCGGTGTTAAGCCGCACGCCGATGGATCGGCAATCACCATTTTGTTGCAGGATAAGGAAGTAGAAGGTCTTCAGTTCATGAAAGATAATGAGTGGTTCAATGCTCCGATTGTTCCGGACGCTCTTCTCGTCAATGTGGGGGATCAAGTAGAGATCACAAGTAATGGGATATTCAAGAGTCCAGTTCATAGAGTATTGACAAATTCAGAGAGGGAGAGGATATCGTTGGCAGTGTTTTACCTGCCAGACGCAGAAAAAGAAATTGAACCATTAGAGGAGCTGATTAGTGAAACTCAGCCAAGGTTGTATAAGAGTGTGAAGAACTTTGTTGGGCTTTACTTTGAATACTACCAACAGGGACAGAGACCAATGGAGGCTGCAAGAATCTAA

Protein sequence

MAAPVIDIALLSSSSKSGPELEKLRHGLQSWGCFQAINHGMTSEYLDEVRRLTKQFFGLSMEEKLKYLKEELEMEGYGNDMILSNQQILDWTDRLYLTVYPHQSRRFKYWPTNLQRFREVIDEYTTNVKLISEKIFKAMARSLDLDESSFLKQYGEQIKLDARFNFYPRCRNPDLVLGVKPHADGSAITILLQDKEVEGLQFMKDNEWFNAPIVPDALLVNVGDQVEITSNGIFKSPVHRVLTNSERERISLAVFYLPDAEKEIEPLEELISETQPRLYKSVKNFVGLYFEYYQQGQRPMEAARI*
Homology
[Close]
BLAST of Cucsa.350520 vs. Swiss-Prot
Match: SRG1_ARATH (Protein SRG1 OS=Arabidopsis thaliana GN=SRG1 PE=2 SV=1)

HSP 1 Score: 211.1 bits (536), Expect = 1.7e-53
Identity = 108/305 (35.41%), Postives = 179/305 (58.69%), Query Frame = 1 

Query: 4   PVIDIALLSSSSKSGPELEKLRHGLQSWGCFQAINHGMTSEYLDEVRRLTKQFFGLSMEE 63
           P+ID+  L SS+    E+EKL    + WG FQ +NHG+ S +LD+V+   + FF L MEE
Sbjct: 54  PIIDMKRLCSSTTMDSEVEKLDFACKEWGFFQLVNHGIDSSFLDKVKSEIQDFFNLPMEE 113

Query: 64  KLKYLKEELEMEGYGNDMILSNQQILDWTDRLYLTVYPHQSRRFKYWPTNLQRFREVIDE 123
           K K+ +   E+EG+G   ++S  Q LDW D  + TV P + R+   +P     FR+ ++ 
Sbjct: 114 KKKFWQRPDEIEGFGQAFVVSEDQKLDWADLFFHTVQPVELRKPHLFPKLPLPFRDTLEM 173

Query: 124 YTTNVKLISEKIFKAMARSLDLDESSFLKQYGEQIKLDA-RFNFYPRCRNPDLVLGVKPH 183
           Y++ V+ +++ +   MAR+L++      K + +   + + R N+YP C  PD V+G+ PH
Sbjct: 174 YSSEVQSVAKILIAKMARALEIKPEELEKLFDDVDSVQSMRMNYYPPCPQPDQVIGLTPH 233

Query: 184 ADGSAITILLQDKEVEGLQFMKDNEWFNAPIVPDALLVNVGDQVEITSNGIFKSPVHRVL 243
           +D   +T+L+Q  +VEGLQ  KD +W     +P+A +VN+GD +EI +NG ++S  HR +
Sbjct: 234 SDSVGLTVLMQVNDVEGLQIKKDGKWVPVKPLPNAFIVNIGDVLEIITNGTYRSIEHRGV 293

Query: 244 TNSERERISLAVFYLPDAEKEIEPLEELISETQPRLYK--SVKNFVGLYFEYYQQGQRPM 303
            NSE+ER+S+A F+     KE+ P + L+   +   +K  ++K +    F     G+  +
Sbjct: 294 VNSEKERLSIATFHNVGMYKEVGPAKSLVERQKVARFKRLTMKEYNDGLFSRTLDGKAYL 353

Query: 304 EAARI 306
           +A RI
Sbjct: 354 DALRI 358

[Close]
BLAST of Cucsa.350520 vs. Swiss-Prot
Match: DIOX3_PAPSO (Codeine O-demethylase OS=Papaver somniferum GN=CODM PE=1 SV=1)

HSP 1 Score: 204.9 bits (520), Expect = 1.2e-51
Identity = 111/279 (39.78%), Postives = 161/279 (57.71%), Query Frame = 1 

Query: 4   PVIDIA-LLSSSSKSGP-ELEKLRHGLQSWGCFQAINHGMTSEYLDEVRRLTKQFFGLSM 63
           PVID+  LLS     G  EL+KL    + WG FQ +NHG+ +  +D ++   K FF L M
Sbjct: 55  PVIDLQNLLSPEPVVGKLELDKLHSACKEWGFFQLVNHGVDALLMDNIKSEIKGFFNLPM 114

Query: 64  EEKLKYLKEELEMEGYGNDMILSNQQILDWTDRLYLTVYPHQSRRFKYWPTNLQRFREVI 123
            EK KY +++ + EG+G   I S  Q LDWT+   +   P   R+   +P     FRE +
Sbjct: 115 NEKTKYGQQDGDFEGFGQPYIESEDQRLDWTEVFSMLSLPLHLRKPHLFPELPLPFRETL 174

Query: 124 DEYTTNVKLISEKIFKAMARSLDLDESSFLKQYGEQIKLDARFNFYPRCRNPDLVLGVKP 183
           + Y + +K +S  +F+ + +SL L E   +    E      R N+YP C  P+LVLG+  
Sbjct: 175 ESYLSKMKKLSTVVFEMLEKSLQLVEIKGMTDLFEDGLQTMRMNYYPPCPRPELVLGLTS 234

Query: 184 HADGSAITILLQDKEVEGLQFMKDNEWFNAPIVPDALLVNVGDQVEITSNGIFKSPVHRV 243
           H+D S +TILLQ  EVEGLQ  K+  W +   +PDA +VNVGD +EI +NGI++S  HR 
Sbjct: 235 HSDFSGLTILLQLNEVEGLQIRKEERWISIKPLPDAFIVNVGDILEIMTNGIYRSVEHRA 294

Query: 244 LTNSERERISLAVFYLPDAEKEIEPLEELISETQPRLYK 281
           + NS +ER+S+A F+    E EI P+  L++   P L+K
Sbjct: 295 VVNSTKERLSIATFHDSKLESEIGPISSLVTPETPALFK 333

[Close]
BLAST of Cucsa.350520 vs. Swiss-Prot
Match: DIOX2_PAPSO (Probable 2-oxoglutarate/Fe(II)-dependent dioxygenase OS=Papaver somniferum GN=DIOX2 PE=2 SV=1)

HSP 1 Score: 202.6 bits (514), Expect = 6.2e-51
Identity = 106/296 (35.81%), Postives = 170/296 (57.43%), Query Frame = 1 

Query: 4   PVIDIALLSSSSKSGP--ELEKLRHGLQSWGCFQAINHGMTSEYLDEVRRLTKQFFGLSM 63
           PVIDI  L SS       EL++L    + WG FQ +NHG+ +  +D V+   + FF LSM
Sbjct: 56  PVIDIENLISSEPVTEKLELDRLHSACKEWGFFQVVNHGVDTSLVDNVKSDIQGFFNLSM 115

Query: 64  EEKLKYLKEELEMEGYGNDMILSNQQILDWTDRLYLTVYPHQSRRFKYWPTNLQRFREVI 123
            EK+KY +++ ++EG+G   + S  Q LDW D   +   P   R+   +       RE I
Sbjct: 116 NEKIKYGQKDGDVEGFGQAFVASEDQTLDWADIFMILTLPLHLRKPHLFSKLPLPLRETI 175

Query: 124 DEYTTNVKLISEKIFKAMARSLDLD--ESSFLKQYGEQIKLDARFNFYPRCRNPDLVLGV 183
           + Y++ +K +S  +F+ M ++L +   E   + +  + +    R N+YP C  P+L +G+
Sbjct: 176 ESYSSEMKKLSMVLFEKMEKALQVQAVEIKEISEVFKDMTQVMRMNYYPPCPQPELAIGL 235

Query: 184 KPHADGSAITILLQDKEVEGLQFMKDNEWFNAPIVPDALLVNVGDQVEITSNGIFKSPVH 243
            PH+D   +TILLQ  EVEGLQ   +  W +   +P+A +VNVGD +EI +NG+++S  H
Sbjct: 236 TPHSDFGGLTILLQLNEVEGLQIKNEGRWISVKPLPNAFVVNVGDVLEIMTNGMYRSVDH 295

Query: 244 RVLTNSERERISLAVFYLPDAEKEIEPLEELISETQPRLYKSVKNFVGLYFEYYQQ 296
           R + NS +ER+S+A F+ P+ E EI P+  LI+   P L++S   +  L  E++ +
Sbjct: 296 RAVVNSTKERLSIATFHDPNLESEIGPISSLITPNTPALFRSGSTYGELVEEFHSR 351

[Close]
BLAST of Cucsa.350520 vs. Swiss-Prot
Match: DIOX1_PAPSO (Thebaine 6-O-demethylase OS=Papaver somniferum GN=T6ODM PE=1 SV=1)

HSP 1 Score: 193.7 bits (491), Expect = 2.9e-48
Identity = 111/294 (37.76%), Postives = 167/294 (56.80%), Query Frame = 1 

Query: 4   PVIDIA-LLSSSSKSGP-ELEKLRHGLQSWGCFQAINHGMTSEYLDEVRRLTKQFFGLSM 63
           PVIDI  LLS     G  EL++L    + WG FQ +NHG+ +  +D V+   + FF LSM
Sbjct: 56  PVIDIENLLSPEPIIGKLELDRLHFACKEWGFFQVVNHGVDASLVDSVKSEIQGFFNLSM 115

Query: 64  EEKLKYLKEELEMEGYGNDMILSNQQILDWTDRLYLTVYPHQSRRFKYWPTNLQRFREVI 123
           +EK KY +E+ ++EG+G   I S  Q LDW D   +   P   R+   +       RE I
Sbjct: 116 DEKTKYEQEDGDVEGFGQGFIESEDQTLDWADIFMMFTLPLHLRKPHLFSKLPVPLRETI 175

Query: 124 DEYTTNVKLISEKIFKAMARSLDLDESSFLKQYGEQIKLDA----RFNFYPRCRNPDLVL 183
           + Y++ +K +S  +F  M ++L + +++ +K   E + +D     R N+YP C  P+L +
Sbjct: 176 ESYSSEMKKLSMVLFNKMEKALQV-QAAEIKGMSE-VFIDGTQAMRMNYYPPCPQPNLAI 235

Query: 184 GVKPHADGSAITILLQDKEVEGLQFMKDNEWFNAPIVPDALLVNVGDQVEITSNGIFKSP 243
           G+  H+D   +TILLQ  EVEGLQ  ++  W +   +P+A +VNVGD +EI +NGI+ S 
Sbjct: 236 GLTSHSDFGGLTILLQINEVEGLQIKREGTWISVKPLPNAFVVNVGDILEIMTNGIYHSV 295

Query: 244 VHRVLTNSERERISLAVFYLPDAEKEIEPLEELISETQPRLYKSVKNFVGLYFE 292
            HR + NS  ER+S+A F+ P  E  I P+  LI+   P L+KS   +  L  E
Sbjct: 296 DHRAVVNSTNERLSIATFHDPSLESVIGPISSLITPETPALFKSGSTYGDLVEE 347

[Close]
BLAST of Cucsa.350520 vs. Swiss-Prot
Match: LDOX_MALDO (Leucoanthocyanidin dioxygenase OS=Malus domestica GN=ANS PE=2 SV=1)

HSP 1 Score: 191.0 bits (484), Expect = 1.9e-47
Identity = 101/283 (35.69%), Postives = 162/283 (57.24%), Query Frame = 1 

Query: 4   PVIDIALLSSSSKS--GPELEKLRHGLQSWGCFQAINHGMTSEYLDEVRRLTKQFFGLSM 63
           P ID+  + S ++       EKL+     WG    +NHG++ E +D+VR+  K FF L +
Sbjct: 52  PTIDLKEIESDNEKVRAKCREKLKKAAVDWGVMHLVNHGISDELMDKVRKAGKAFFDLPI 111

Query: 64  EEKLKYLKEEL--EMEGYGNDMILSNQQILDWTDRLYLTVYPHQSRRFKYWPTNLQRFRE 123
           E+K KY  ++   +++GYG+ +  +    L+W D  +  VYP   R    WP     + E
Sbjct: 112 EQKEKYANDQASGKIQGYGSKLANNASGQLEWEDYFFHCVYPEDKRDLSIWPQTPADYIE 171

Query: 124 VIDEYTTNVKLISEKIFKAMARSLDLDESSFLKQYG--EQIKLDARFNFYPRCRNPDLVL 183
              EY   ++ ++ K+ K ++  L LDE    K+ G  E++ L  + N+YP+C  P+L L
Sbjct: 172 ATAEYAKQLRELATKVLKVLSLGLGLDEGRLEKEVGGLEELLLQMKINYYPKCPQPELAL 231

Query: 184 GVKPHADGSAITILLQDKEVEGLQFMKDNEWFNAPIVPDALLVNVGDQVEITSNGIFKSP 243
           GV+ H D SA+T +L +  V GLQ   + +W  A  VP+++++++GD +EI SNG +KS 
Sbjct: 232 GVEAHTDVSALTFILHNM-VPGLQLFYEGKWVTAKCVPNSIVMHIGDTLEILSNGKYKSI 291

Query: 244 VHRVLTNSERERISLAVFYLPDAEKEI-EPLEELISETQPRLY 280
           +HR + N E+ RIS AVF  P  EK I +PL E +SE +P ++
Sbjct: 292 LHRGMVNKEKVRISWAVFCEPPKEKIILKPLPETVSEDEPAMF 333

[Close]
BLAST of Cucsa.350520 vs. TrEMBL
Match: A0A0A0LYX6_CUCSA (Uncharacterized protein OS=Cucumis sativus GN=Csa_1G263950 PE=3 SV=1)

HSP 1 Score: 604.4 bits (1557), Expect = 7.9e-170
Identity = 302/305 (99.02%), Postives = 302/305 (99.02%), Query Frame = 1 

Query: 1   MAAPVIDIALLSSSSKSGPELEKLRHGLQSWGCFQAINHGMTSEYLDEVRRLTKQFFGLS 60
           MAAPVIDIALLSSSSKSGPELEKLRHGLQSWGCFQAINHGMTSEYLDEVRRLTKQFFGLS
Sbjct: 38  MAAPVIDIALLSSSSKSGPELEKLRHGLQSWGCFQAINHGMTSEYLDEVRRLTKQFFGLS 97

Query: 61  MEEKLKYLKEELEMEGYGNDMILSNQQILDWTDRLYLTVYPHQSRRFKYWPTNLQRFREV 120
           MEEKLKYLKEELEMEGYGNDMILSNQQILDWTDRLYLTVYPHQSRRFKYWPTNLQRFREV
Sbjct: 98  MEEKLKYLKEELEMEGYGNDMILSNQQILDWTDRLYLTVYPHQSRRFKYWPTNLQRFREV 157

Query: 121 IDEYTTNVKLISEKIFKAMARSLDLDESSFLKQYGEQIKLDARFNFYPRCRNPDLVLGVK 180
           IDEYTTNVKLISEKIFKAMARSLDLDESSFLKQYGEQIKL ARFNFYPRCRNPDLVLGVK
Sbjct: 158 IDEYTTNVKLISEKIFKAMARSLDLDESSFLKQYGEQIKLGARFNFYPRCRNPDLVLGVK 217

Query: 181 PHADGSAITILLQDKEVEGLQFMKDNEWFNAPIVPDALLVNVGDQVEITSNGIFKSPVHR 240
           PHADGSAITILLQDKEVEGLQFMKDNEWFNA IVPDALLVNVGDQVEITSNGIFKSPVHR
Sbjct: 218 PHADGSAITILLQDKEVEGLQFMKDNEWFNASIVPDALLVNVGDQVEITSNGIFKSPVHR 277

Query: 241 VLTNSERERISLAVFYLPDAEKEIEPLEELISETQPRLYKSVKNFVGLYFEYYQQGQRPM 300
           VLTNSERERISLAVFYLPDAEKEIEPLEELISETQPRLYKSVKNFVGL FEYYQQGQRPM
Sbjct: 278 VLTNSERERISLAVFYLPDAEKEIEPLEELISETQPRLYKSVKNFVGLSFEYYQQGQRPM 337

Query: 301 EAARI 306
           EAARI
Sbjct: 338 EAARI 342

[Close]
BLAST of Cucsa.350520 vs. TrEMBL
Match: A0A096ZJT4_9ROSA (EFE OS=Pyrus x bretschneideri GN=EFE PE=2 SV=1)

HSP 1 Score: 425.2 bits (1092), Expect = 6.5e-116
Identity = 196/306 (64.05%), Postives = 255/306 (83.33%), Query Frame = 1 

Query: 1   MAAPVIDIALLSSSSKSGPELEKLRHGLQSWGCFQAINHGMTSEYLDEVRRLTKQFFGLS 60
           M  PVID+ALL++SS S  ELEKLR  L +WGCFQ INHGM+ E+LDE R +TKQFF L 
Sbjct: 43  MDVPVIDLALLAASSISADELEKLRSALATWGCFQVINHGMSPEFLDEFREITKQFFALP 102

Query: 61  MEEKLKYLKEELEMEGYGNDMILSNQQILDWTDRLYLTVYPHQSRRFKYWPTNLQRFREV 120
           +E+K KYL++  +++GYGNDM+ S QQ LDW+DRLYL+VYP  +R+   WP N + FR+ 
Sbjct: 103 VEDKKKYLRQVNDIQGYGNDMVFSEQQTLDWSDRLYLSVYPQDNRKLNLWPENPKSFRQT 162

Query: 121 IDEYTTNVKLISEKIFKAMARSLDLDESSFLKQYG-EQIKLDARFNFYPRCRNPDLVLGV 180
           +D+YT +++++++ + KAMARSLDL+++ F   YG EQ K+D RFNFYP C  PDLVLGV
Sbjct: 163 LDQYTMDLQVVTKTVLKAMARSLDLEDNCFSDLYGGEQGKMDIRFNFYPPCPRPDLVLGV 222

Query: 181 KPHADGSAITILLQDKEVEGLQFMKDNEWFNAPIVPDALLVNVGDQVEITSNGIFKSPVH 240
           KPHADG+ IT+LLQDKEVEGLQF+K ++WF APIVPDALL+NVGDQVEI SNGIFKSPVH
Sbjct: 223 KPHADGTLITLLLQDKEVEGLQFLKGDKWFRAPIVPDALLINVGDQVEILSNGIFKSPVH 282

Query: 241 RVLTNSERERISLAVFYLPDAEKEIEPLEELISETQPRLYKSVKNFVGLYFEYYQQGQRP 300
           +V+TNS++ERIS+A F +P+++KEIEP E L++E++PRLYK VKN+VG+YFEYYQQG+RP
Sbjct: 283 KVVTNSDKERISVAAFCIPESDKEIEPFERLVNESRPRLYKKVKNYVGIYFEYYQQGRRP 342

Query: 301 MEAARI 306
           MEAA++
Sbjct: 343 MEAAKM 348

[Close]
BLAST of Cucsa.350520 vs. TrEMBL
Match: M5X0W8_PRUPE (Uncharacterized protein OS=Prunus persica GN=PRUPE_ppa007944mg PE=3 SV=1)

HSP 1 Score: 419.9 bits (1078), Expect = 2.7e-114
Identity = 193/306 (63.07%), Postives = 251/306 (82.03%), Query Frame = 1 

Query: 1   MAAPVIDIALL-SSSSKSGPELEKLRHGLQSWGCFQAINHGMTSEYLDEVRRLTKQFFGL 60
           M  PVID+ LL + SS +  +LEKLR  L +WGCFQ INHGMT E+LDEVR +TKQFF L
Sbjct: 45  MDIPVIDLGLLLTPSSITAQQLEKLRSALTTWGCFQVINHGMTPEFLDEVREMTKQFFAL 104

Query: 61  SMEEKLKYLKEELEMEGYGNDMILSNQQILDWTDRLYLTVYPHQSRRFKYWPTNLQRFRE 120
            +EEK KYL++  +++GYGNDM+ S QQ LDW+DRLYL+VYP + R+ K+WP N + F E
Sbjct: 105 PVEEKQKYLRQVNDIQGYGNDMVFSEQQTLDWSDRLYLSVYPEEHRKLKFWPQNPKSFSE 164

Query: 121 VIDEYTTNVKLISEKIFKAMARSLDLDESSFLKQYGEQIKLDARFNFYPRCRNPDLVLGV 180
            +D+YT  ++++++ + +AMARSL+LD + F   YGEQ K+D RFNFYP C  PD+VLGV
Sbjct: 165 TLDQYTMKLQVVTKTVLEAMARSLNLDVNCFRDLYGEQGKMDVRFNFYPPCSRPDVVLGV 224

Query: 181 KPHADGSAITILLQDKEVEGLQFMKDNEWFNAPIVPDALLVNVGDQVEITSNGIFKSPVH 240
           KPHADG+ IT+LLQDK+VEGLQF+KD++WF APIVP+ALL+NVGDQ EI SNGIFKSPVH
Sbjct: 225 KPHADGTIITLLLQDKQVEGLQFLKDDQWFRAPIVPEALLINVGDQAEILSNGIFKSPVH 284

Query: 241 RVLTNSERERISLAVFYLPDAEKEIEPLEELISETQPRLYKSVKNFVGLYFEYYQQGQRP 300
           RV+TN ++ERISLA F +P+++K+IEP E L++E+ PRLYK VKN+VG+YFEYYQQG+RP
Sbjct: 285 RVVTNPDKERISLAAFCIPESDKDIEPFESLVNESTPRLYKKVKNYVGIYFEYYQQGRRP 344

Query: 301 MEAARI 306
           +EAA+I
Sbjct: 345 IEAAKI 350

[Close]
BLAST of Cucsa.350520 vs. TrEMBL
Match: W9RPD3_9ROSA (Protein SRG1 OS=Morus notabilis GN=L484_023672 PE=3 SV=1)

HSP 1 Score: 416.8 bits (1070), Expect = 2.3e-113
Identity = 194/305 (63.61%), Postives = 248/305 (81.31%), Query Frame = 1 

Query: 1   MAAPVIDIALLSSSSKSGPELEKLRHGLQSWGCFQAINHGMTSEYLDEVRRLTKQFFGLS 60
           M  PV+DIALL SS++   EL+KLR  L +WGCFQAINHGM+ E+L++VR +TK+FF L 
Sbjct: 37  MDVPVVDIALLGSSTE---ELDKLRSALNTWGCFQAINHGMSPEFLNQVRGITKEFFHLP 96

Query: 61  MEEKLKYLKEELEMEGYGNDMILSNQQILDWTDRLYLTVYPHQSRRFKYWPTNLQRFREV 120
            EEK  YL+EE ++EGYGNDM+L  QQ LDWTDRLYL +YP   R+ ++WP   + FR  
Sbjct: 97  FEEKQLYLREENDIEGYGNDMVLWEQQKLDWTDRLYLAIYPEDQRKLRFWPEKPEAFRST 156

Query: 121 IDEYTTNVKLISEKIFKAMARSLDLDESSFLKQYGEQIKLDARFNFYPRCRNPDLVLGVK 180
           + EY+  +K I   + KAMARSL L+E+ F+ QYGE+ KL ARFNFYP+C  PDLVLG K
Sbjct: 157 LHEYSLKLKSIGVVVLKAMARSLSLEENCFVDQYGERSKLHARFNFYPKCPRPDLVLGTK 216

Query: 181 PHADGSAITILLQDKEVEGLQFMKDNEWFNAPIVPDALLVNVGDQVEITSNGIFKSPVHR 240
           PHADG+ IT+LLQD+ VEGLQFMK ++W+ API+P+ALL+NVGDQ EI+SNG+FKSPVHR
Sbjct: 217 PHADGTGITLLLQDESVEGLQFMKADKWYRAPIIPEALLINVGDQAEISSNGMFKSPVHR 276

Query: 241 VLTNSERERISLAVFYLPDAEKEIEPLEELISETQPRLYKSVKNFVGLYFEYYQQGQRPM 300
           V+TN+ERERISLAVFY+PD E +IEP EELI+E++PRLY+ VKN+V +YF+YYQ+G+RPM
Sbjct: 277 VVTNAERERISLAVFYVPDPENDIEPFEELINESRPRLYRKVKNYVDIYFQYYQKGRRPM 336

Query: 301 EAARI 306
           EAA+I
Sbjct: 337 EAAKI 338

[Close]
BLAST of Cucsa.350520 vs. TrEMBL
Match: M5WFY4_PRUPE (Uncharacterized protein OS=Prunus persica GN=PRUPE_ppa007960mg PE=3 SV=1)

HSP 1 Score: 414.8 bits (1065), Expect = 8.8e-113
Identity = 191/306 (62.42%), Postives = 250/306 (81.70%), Query Frame = 1 

Query: 1   MAAPVIDIALL-SSSSKSGPELEKLRHGLQSWGCFQAINHGMTSEYLDEVRRLTKQFFGL 60
           M  PVID+ LL + SS +  +LEKLR  L +WGCFQ INHGMT E+LD+VR +TKQFF L
Sbjct: 45  MDIPVIDLGLLLTPSSITAQQLEKLRSALTTWGCFQVINHGMTPEFLDKVREMTKQFFAL 104

Query: 61  SMEEKLKYLKEELEMEGYGNDMILSNQQILDWTDRLYLTVYPHQSRRFKYWPTNLQRFRE 120
            +EEK KYL++  +++GYGNDM+ S QQ LDW+DRLYL+VYP + R+ K+WP N + F E
Sbjct: 105 PLEEKQKYLRQINDIQGYGNDMVFSEQQTLDWSDRLYLSVYPEEHRKLKFWPQNPKSFSE 164

Query: 121 VIDEYTTNVKLISEKIFKAMARSLDLDESSFLKQYGEQIKLDARFNFYPRCRNPDLVLGV 180
            +D+YT  ++++++ + +AMARSL+LD + F    GEQ K+D RFNFYP C  PD+VLGV
Sbjct: 165 TLDQYTMKLQVVTKTVLEAMARSLNLDVNCFRDLCGEQGKMDVRFNFYPPCSRPDVVLGV 224

Query: 181 KPHADGSAITILLQDKEVEGLQFMKDNEWFNAPIVPDALLVNVGDQVEITSNGIFKSPVH 240
           KPHADG+ IT+LLQDK+VEGLQF+KD++WF APIVP+ALL+NVGDQ EI SNGIFKSPVH
Sbjct: 225 KPHADGTIITLLLQDKQVEGLQFLKDDQWFRAPIVPEALLINVGDQAEILSNGIFKSPVH 284

Query: 241 RVLTNSERERISLAVFYLPDAEKEIEPLEELISETQPRLYKSVKNFVGLYFEYYQQGQRP 300
           RV+TN ++ERISLA F +P+++K+IEP E L++E+ PRLYK VKN+VG+YFEYYQQG+RP
Sbjct: 285 RVVTNPDKERISLAAFCIPESDKDIEPFESLVNESTPRLYKKVKNYVGIYFEYYQQGRRP 344

Query: 301 MEAARI 306
           +EAA+I
Sbjct: 345 IEAAKI 350

[Close]
BLAST of Cucsa.350520 vs. TAIR10
Match: AT5G20400.1 (AT5G20400.1 2-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily protein)

HSP 1 Score: 380.6 bits (976), Expect = 9.3e-106
Identity = 181/306 (59.15%), Postives = 232/306 (75.82%), Query Frame = 1 

Query: 1   MAAPVIDIALLSSSSKSGP-ELEKLRHGLQSWGCFQAINHGMTSEYLDEVRRLTKQFFGL 60
           M  P ID+ LL SSS++G  EL KL   L +WG  Q +NHG+T  +LD++ +LTK+FF L
Sbjct: 43  MDIPAIDLNLLLSSSEAGQQELSKLHSALSTWGVVQVMNHGITKAFLDKIYKLTKEFFAL 102

Query: 61  SMEEKLKYLKEELEMEGYGNDMILSNQQILDWTDRLYLTVYPHQSRRFKYWPTNLQRFRE 120
             EEK K  +E   ++GYGNDMIL + Q+LDW DRLY+T YP   R+  +WP     FRE
Sbjct: 103 PTEEKQKCAREIDSIQGYGNDMILWDDQVLDWIDRLYITTYPEDQRQLNFWPEVPLGFRE 162

Query: 121 VIDEYTTNVKLISEKIFKAMARSLDLDESSFLKQYGEQIKLDARFNFYPRCRNPDLVLGV 180
            + EYT   +++ E+ FKAMARSL+L+E+SFL  YGE   LD RFN YP C +PD V+GV
Sbjct: 163 TLHEYTMKQRIVIEQFFKAMARSLELEENSFLDMYGESATLDTRFNMYPPCPSPDKVIGV 222

Query: 181 KPHADGSAITILLQDKEVEGLQFMKDNEWFNAPIVPDALLVNVGDQVEITSNGIFKSPVH 240
           KPHADGSAIT+LL DK+V GLQF KD +W+ APIVPD +L+NVGDQ+EI SNGI+KSPVH
Sbjct: 223 KPHADGSAITLLLPDKDVGGLQFQKDGKWYKAPIVPDTILINVGDQMEIMSNGIYKSPVH 282

Query: 241 RVLTNSERERISLAVFYLPDAEKEIEPLEELISETQPRLYKSVKNFVGLYFEYYQQGQRP 300
           RV+TN E+ERIS+A F +P A+KEI+P+ EL+SE +PRLYK+VK +V LYF+YYQQG+RP
Sbjct: 283 RVVTNREKERISVATFCIPGADKEIQPVNELVSEARPRLYKTVKKYVELYFKYYQQGRRP 342

Query: 301 MEAARI 306
           +EAA I
Sbjct: 343 IEAALI 348

[Close]
BLAST of Cucsa.350520 vs. TAIR10
Match: AT1G49390.1 (AT1G49390.1 2-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily protein)

HSP 1 Score: 367.5 bits (942), Expect = 8.2e-102
Identity = 172/306 (56.21%), Postives = 231/306 (75.49%), Query Frame = 1 

Query: 1   MAAPVIDIALLSSSSKSGPE-LEKLRHGLQSWGCFQAINHGMTSEYLDEVRRLTKQFFGL 60
           M  P ID++LL SSS  G E ++KL   L +WG  Q +NHG+T  +LD++ +LTKQFF L
Sbjct: 43  MDIPAIDLSLLFSSSVDGQEEMKKLHSALSTWGVVQVMNHGITEAFLDKIYKLTKQFFAL 102

Query: 61  SMEEKLKYLKEELEMEGYGNDMILSNQQILDWTDRLYLTVYPHQSRRFKYWPTNLQRFRE 120
             EEK K  +E   ++GYGNDMILS+ Q+LDW DRL+LT YP   R+ K+WP     F E
Sbjct: 103 PTEEKHKCARETGNIQGYGNDMILSDNQVLDWIDRLFLTTYPEDKRQLKFWPQVPVGFSE 162

Query: 121 VIDEYTTNVKLISEKIFKAMARSLDLDESSFLKQYGEQIKLDARFNFYPRCRNPDLVLGV 180
            +DEYT   +++ EK FKAMARSL+L+E+ FL+ YGE   +++RFNF+P C  PD V+G+
Sbjct: 163 TLDEYTMKQRVLIEKFFKAMARSLELEENCFLEMYGENAVMNSRFNFFPPCPRPDKVIGI 222

Query: 181 KPHADGSAITILLQDKEVEGLQFMKDNEWFNAPIVPDALLVNVGDQVEITSNGIFKSPVH 240
           KPHADGSAIT+LL DK+VEGLQF+KD +W+ APIVPD +L+ +GDQ+EI SNGI+KSPVH
Sbjct: 223 KPHADGSAITLLLPDKDVEGLQFLKDGKWYKAPIVPDTILITLGDQMEIMSNGIYKSPVH 282

Query: 241 RVLTNSERERISLAVFYLPDAEKEIEPLEELISETQPRLYKSVKNFVGLYFEYYQQGQRP 300
           RV+TN E+ERIS+A F +P  +KEI P + L++E +PRLYK+V  +V L+++YYQQG+R 
Sbjct: 283 RVVTNREKERISVATFCVPGLDKEIHPADGLVTEARPRLYKTVTKYVDLHYKYYQQGRRT 342

Query: 301 MEAARI 306
           +EAA I
Sbjct: 343 IEAALI 348

[Close]
BLAST of Cucsa.350520 vs. TAIR10
Match: AT5G20550.1 (AT5G20550.1 2-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily protein)

HSP 1 Score: 347.8 bits (891), Expect = 6.7e-96
Identity = 170/307 (55.37%), Postives = 220/307 (71.66%), Query Frame = 1 

Query: 1   MAAPVIDIALLSSSSKSG-PELEKLRHGLQSWGCFQAINHGMTSEYLDEVRRLTKQFFGL 60
           M  P ID++LL S S  G  EL KL   L +WG  Q INHG+T   LD++ +LTK+F  L
Sbjct: 43  MDIPAIDLSLLLSPSDDGREELSKLHSALSTWGVVQVINHGITKALLDKIYKLTKEFCAL 102

Query: 61  SMEEKLKYLKEELEMEGYGNDMILSNQQILDWTDRLYLTVYPHQSRRFKYWPTNLQRFRE 120
             EEK KY +E   ++GYGNDMIL + Q+LDW DRLY+T YP   R+ K+WP     FRE
Sbjct: 103 PSEEKQKYAREIGSIQGYGNDMILWDDQVLDWIDRLYITTYPEDQRQLKFWPDVPVGFRE 162

Query: 121 VIDEYTTNVKLISEKIFKAMARSLDLDESSFLKQYGEQIKLDARFNFYPRCRNPDLVLGV 180
            + EYT    L+  ++FKAMA SL+L+E+ FL   GE   +D RFN YP C  PD V+GV
Sbjct: 163 TLHEYTMKQHLVFNQVFKAMAISLELEENCFLDMCGENATMDTRFNMYPPCPRPDKVIGV 222

Query: 181 KPHADGSAITILLQDKEVEGLQFMKDNEWFNAPIV-PDALLVNVGDQVEITSNGIFKSPV 240
           +PHAD SA T+LL DK VEGLQF+KD +W+ AP+V  D +L+NVGDQ+EI SNGI+KSPV
Sbjct: 223 RPHADKSAFTLLLPDKNVEGLQFLKDGKWYKAPVVASDTILINVGDQMEIMSNGIYKSPV 282

Query: 241 HRVLTNSERERISLAVFYLPDAEKEIEPLEELISETQPRLYKSVKNFVGLYFEYYQQGQR 300
           HRV+TN+E+ERIS+A F +P A+KEI+P++ L+SE +PRLYK VKN+V L  +YY QGQR
Sbjct: 283 HRVVTNTEKERISVATFCIPGADKEIQPVDGLVSEARPRLYKPVKNYVDLLNKYYIQGQR 342

Query: 301 PMEAARI 306
           P+ A+ I
Sbjct: 343 PIAASLI 349

[Close]
BLAST of Cucsa.350520 vs. TAIR10
Match: AT5G54000.1 (AT5G54000.1 2-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily protein)

HSP 1 Score: 347.1 bits (889), Expect = 1.1e-95
Identity = 164/306 (53.59%), Postives = 218/306 (71.24%), Query Frame = 1 

Query: 1   MAAPVIDIALLSSSSKSG-PELEKLRHGLQSWGCFQAINHGMTSEYLDEVRRLTKQFFGL 60
           M   +ID+ LL SSS  G  EL KL   + +WG  Q +NHG++   LD++  LTKQFF L
Sbjct: 44  MKISIIDLNLLFSSSDDGREELSKLHSAISTWGVVQVMNHGISEALLDKIHELTKQFFVL 103

Query: 61  SMEEKLKYLKEELEMEGYGNDMILSNQQILDWTDRLYLTVYPHQSRRFKYWPTNLQRFRE 120
             +EK KY +E    +G+GNDMILS+ Q+LDW DRLYL  YP   R+ K+WP N   FRE
Sbjct: 104 PTKEKQKYAREISSFQGFGNDMILSDDQVLDWVDRLYLITYPEDQRQLKFWPENPSGFRE 163

Query: 121 VIDEYTTNVKLISEKIFKAMARSLDLDESSFLKQYGEQIKLDARFNFYPRCRNPDLVLGV 180
            + EYT   +L+ EK FKA+ARSL+L+++ FL+ +GE   L+ RFN YP C  PD VLG+
Sbjct: 164 TLHEYTMKQQLVVEKFFKALARSLELEDNCFLEMHGENATLETRFNIYPPCPRPDKVLGL 223

Query: 181 KPHADGSAITILLQDKEVEGLQFMKDNEWFNAPIVPDALLVNVGDQVEITSNGIFKSPVH 240
           KPH+DGSA T++L DK VEGLQF+KD +W+ A I+P  +L+NVGD +E+ SNGI+KSPVH
Sbjct: 224 KPHSDGSAFTLILPDKNVEGLQFLKDGKWYKASILPHTILINVGDTMEVMSNGIYKSPVH 283

Query: 241 RVLTNSERERISLAVFYLPDAEKEIEPLEELISETQPRLYKSVKNFVGLYFEYYQQGQRP 300
           RV+ N ++ERI +A F   D +KEI+PL  L+SE +PRLYK+VK     +F+YYQQG+RP
Sbjct: 284 RVVLNGKKERIYVATFCNADEDKEIQPLNGLVSEARPRLYKAVKKSEKNFFDYYQQGRRP 343

Query: 301 MEAARI 306
           +EAA I
Sbjct: 344 IEAAMI 349

[Close]
BLAST of Cucsa.350520 vs. TAIR10
Match: AT3G21420.1 (AT3G21420.1 2-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily protein)

HSP 1 Score: 237.7 bits (605), Expect = 9.8e-63
Identity = 125/310 (40.32%), Postives = 188/310 (60.65%), Query Frame = 1 

Query: 4   PVIDIALLSSSSKSGP--ELEKLRHGLQSWGCFQAINHGMTSEYLDEVRRLTKQFFGLSM 63
           PVID++ LS         E+ KL    + WG FQ INHG+  E ++++  +  +FF + +
Sbjct: 56  PVIDLSKLSKPDNDDFFFEILKLSQACEDWGFFQVINHGIEVEVVEDIEEVASEFFDMPL 115

Query: 64  EEKLKYLKEELEMEGYGNDMILSNQQILDWTDRLYLTVYPHQSRRFKYWPTNLQRFREVI 123
           EEK KY  E   ++GYG   I S  Q LDW +   L V+P Q R  K WP+   RF E +
Sbjct: 116 EEKKKYPMEPGTVQGYGQAFIFSEDQKLDWCNMFALGVHPPQIRNPKLWPSKPARFSESL 175

Query: 124 DEYTTNVKLISEKIFKAMARSLDLDESSFLKQYGEQIKLDARFNFYPRCRNPDLVLGVKP 183
           + Y+  ++ + +++ K +A SL L E  F + +GE ++   R N+YP C +PDLVLG+ P
Sbjct: 176 EGYSKEIRELCKRLLKYIAISLGLKEERFEEMFGEAVQA-VRMNYYPPCSSPDLVLGLSP 235

Query: 184 HADGSAITILLQDK-EVEGLQFMKDNEWFNAPIVPDALLVNVGDQVEITSNGIFKSPVHR 243
           H+DGSA+T+L Q K    GLQ +KDN W     +P+AL++N+GD +E+ SNG +KS  HR
Sbjct: 236 HSDGSALTVLQQSKNSCVGLQILKDNTWVPVKPLPNALVINIGDTIEVLSNGKYKSVEHR 295

Query: 244 VLTNSERERISLAVFYLPDAEKEIEPLEELI-SETQPRLYKSVKNFVGLYFEYY----QQ 303
            +TN E+ER+++  FY P+ E EIEP+ EL+  ET P  Y+S  +  G Y  +Y     Q
Sbjct: 296 AVTNREKERLTIVTFYAPNYEVEIEPMSELVDDETNPCKYRSYNH--GDYSYHYVSNKLQ 355

Query: 304 GQRPMEAARI 306
           G++ ++ A+I
Sbjct: 356 GKKSLDFAKI 362

[Close]
BLAST of Cucsa.350520 vs. NCBI nr
Match: gi|778660150|ref|XP_011655688.1| (PREDICTED: protein SRG1-like [Cucumis sativus])

HSP 1 Score: 604.4 bits (1557), Expect = 1.1e-169
Identity = 302/305 (99.02%), Postives = 302/305 (99.02%), Query Frame = 1 

Query: 1   MAAPVIDIALLSSSSKSGPELEKLRHGLQSWGCFQAINHGMTSEYLDEVRRLTKQFFGLS 60
           MAAPVIDIALLSSSSKSGPELEKLRHGLQSWGCFQAINHGMTSEYLDEVRRLTKQFFGLS
Sbjct: 38  MAAPVIDIALLSSSSKSGPELEKLRHGLQSWGCFQAINHGMTSEYLDEVRRLTKQFFGLS 97

Query: 61  MEEKLKYLKEELEMEGYGNDMILSNQQILDWTDRLYLTVYPHQSRRFKYWPTNLQRFREV 120
           MEEKLKYLKEELEMEGYGNDMILSNQQILDWTDRLYLTVYPHQSRRFKYWPTNLQRFREV
Sbjct: 98  MEEKLKYLKEELEMEGYGNDMILSNQQILDWTDRLYLTVYPHQSRRFKYWPTNLQRFREV 157

Query: 121 IDEYTTNVKLISEKIFKAMARSLDLDESSFLKQYGEQIKLDARFNFYPRCRNPDLVLGVK 180
           IDEYTTNVKLISEKIFKAMARSLDLDESSFLKQYGEQIKL ARFNFYPRCRNPDLVLGVK
Sbjct: 158 IDEYTTNVKLISEKIFKAMARSLDLDESSFLKQYGEQIKLGARFNFYPRCRNPDLVLGVK 217

Query: 181 PHADGSAITILLQDKEVEGLQFMKDNEWFNAPIVPDALLVNVGDQVEITSNGIFKSPVHR 240
           PHADGSAITILLQDKEVEGLQFMKDNEWFNA IVPDALLVNVGDQVEITSNGIFKSPVHR
Sbjct: 218 PHADGSAITILLQDKEVEGLQFMKDNEWFNASIVPDALLVNVGDQVEITSNGIFKSPVHR 277

Query: 241 VLTNSERERISLAVFYLPDAEKEIEPLEELISETQPRLYKSVKNFVGLYFEYYQQGQRPM 300
           VLTNSERERISLAVFYLPDAEKEIEPLEELISETQPRLYKSVKNFVGL FEYYQQGQRPM
Sbjct: 278 VLTNSERERISLAVFYLPDAEKEIEPLEELISETQPRLYKSVKNFVGLSFEYYQQGQRPM 337

Query: 301 EAARI 306
           EAARI
Sbjct: 338 EAARI 342

[Close]
BLAST of Cucsa.350520 vs. NCBI nr
Match: gi|659131150|ref|XP_008465535.1| (PREDICTED: protein SRG1-like [Cucumis melo])

HSP 1 Score: 588.2 bits (1515), Expect = 8.4e-165
Identity = 290/305 (95.08%), Postives = 299/305 (98.03%), Query Frame = 1 

Query: 1   MAAPVIDIALLSSSSKSGPELEKLRHGLQSWGCFQAINHGMTSEYLDEVRRLTKQFFGLS 60
           MAAPVIDIALLSSSSKSGPELEKLRHGLQSWGCFQAINHGMTSEYLDEVR+LTKQFFGLS
Sbjct: 57  MAAPVIDIALLSSSSKSGPELEKLRHGLQSWGCFQAINHGMTSEYLDEVRQLTKQFFGLS 116

Query: 61  MEEKLKYLKEELEMEGYGNDMILSNQQILDWTDRLYLTVYPHQSRRFKYWPTNLQRFREV 120
           MEEKLKYLKEE EMEGYGNDMILSNQQILDWTDRLYLTVYPHQSRRFKYWPTN QRFREV
Sbjct: 117 MEEKLKYLKEEHEMEGYGNDMILSNQQILDWTDRLYLTVYPHQSRRFKYWPTNPQRFREV 176

Query: 121 IDEYTTNVKLISEKIFKAMARSLDLDESSFLKQYGEQIKLDARFNFYPRCRNPDLVLGVK 180
           + EYTTNVKLISEKI KAMARSLDLDESSFL QYGEQ+KLDARFNFYPRCRNPDLVLGVK
Sbjct: 177 VGEYTTNVKLISEKILKAMARSLDLDESSFLSQYGEQVKLDARFNFYPRCRNPDLVLGVK 236

Query: 181 PHADGSAITILLQDKEVEGLQFMKDNEWFNAPIVPDALLVNVGDQVEITSNGIFKSPVHR 240
           PHADGSAITILLQDK+VEGLQFMKDNEW+NAPIV DALLVNVGDQVEITSNGIFKSPVHR
Sbjct: 237 PHADGSAITILLQDKKVEGLQFMKDNEWYNAPIVTDALLVNVGDQVEITSNGIFKSPVHR 296

Query: 241 VLTNSERERISLAVFYLPDAEKEIEPLEELISETQPRLYKSVKNFVGLYFEYYQQGQRPM 300
           VLTNSERERISLAVFYLPD+EKEIEPLEELI+E+QPRLYKSVKNFVGLYFEYYQQG+RPM
Sbjct: 297 VLTNSERERISLAVFYLPDSEKEIEPLEELINESQPRLYKSVKNFVGLYFEYYQQGERPM 356

Query: 301 EAARI 306
           EAARI
Sbjct: 357 EAARI 361

[Close]
BLAST of Cucsa.350520 vs. NCBI nr
Match: gi|470101600|ref|XP_004287258.1| (PREDICTED: protein SRG1-like isoform X1 [Fragaria vesca subsp. vesca])

HSP 1 Score: 430.3 bits (1105), Expect = 2.9e-117
Identity = 200/305 (65.57%), Postives = 246/305 (80.66%), Query Frame = 1 

Query: 1   MAAPVIDIALLSSSSKSGPELEKLRHGLQSWGCFQAINHGMTSEYLDEVRRLTKQFFGLS 60
           M  PVID+ LL+ SS S  E EKLR  L  WGCFQ +NHGMT E+LD+VR +TKQFF + 
Sbjct: 50  MDVPVIDLGLLTPSSISMEEFEKLRSALNKWGCFQVVNHGMTPEFLDQVREITKQFFAMP 109

Query: 61  MEEKLKYLKEELEMEGYGNDMILSNQQILDWTDRLYLTVYPHQSRRFKYWPTNLQRFREV 120
            EEK KYL+    +EGYGNDM+ S QQ LDWTDRLYLTVYP    + K+WP N + FR+ 
Sbjct: 110 AEEKKKYLRPVDNIEGYGNDMVFSEQQTLDWTDRLYLTVYPPDKHKLKFWPENPKSFRDT 169

Query: 121 IDEYTTNVKLISEKIFKAMARSLDLDESSFLKQYGEQIKLDARFNFYPRCRNPDLVLGVK 180
           +D+YT  +++I+E + K MARSL+L+E  FLK+YGEQ K+DARFNFYP C  PD VLG K
Sbjct: 170 LDQYTCKLQVITETVLKGMARSLNLEEDCFLKKYGEQRKMDARFNFYPPCSRPDRVLGTK 229

Query: 181 PHADGSAITILLQDKEVEGLQFMKDNEWFNAPIVPDALLVNVGDQVEITSNGIFKSPVHR 240
           PHADG+ IT+LLQDKEVEGLQFMKD+ W+ APIVP+ALL+NVGDQ EI SNG+FKSPVH+
Sbjct: 230 PHADGTLITLLLQDKEVEGLQFMKDDRWYRAPIVPEALLINVGDQAEILSNGLFKSPVHK 289

Query: 241 VLTNSERERISLAVFYLPDAEKEIEPLEELISETQPRLYKSVKNFVGLYFEYYQQGQRPM 300
           V+TN+E+ERISLAVF +PD+E EIEP E LI+E++PRLYK +KN+VG+YFEYYQQG+RP+
Sbjct: 290 VVTNAEKERISLAVFCVPDSETEIEPFESLINESRPRLYKKLKNYVGIYFEYYQQGRRPI 349

Query: 301 EAARI 306
           EAA I
Sbjct: 350 EAAII 354

[Close]
BLAST of Cucsa.350520 vs. NCBI nr
Match: gi|764507132|ref|XP_011457663.1| (PREDICTED: protein SRG1-like isoform X2 [Fragaria vesca subsp. vesca])

HSP 1 Score: 427.6 bits (1098), Expect = 1.9e-116
Identity = 198/305 (64.92%), Postives = 247/305 (80.98%), Query Frame = 1 

Query: 1   MAAPVIDIALLSSSSKSGPELEKLRHGLQSWGCFQAINHGMTSEYLDEVRRLTKQFFGLS 60
           M  PVID+ LL+ SS S  E EKLR  L  WGCFQ +NHGMT E+LD+VR +TKQFF + 
Sbjct: 50  MDVPVIDLGLLTPSSISMEEFEKLRSALNKWGCFQVVNHGMTPEFLDQVREITKQFFAMP 109

Query: 61  MEEKLKYLKEELEMEGYGNDMILSNQQILDWTDRLYLTVYPHQSRRFKYWPTNLQRFREV 120
            EEK KYL+    +EGYGNDM+ S QQ LDWTDRLYLTVYP   R+ K+WP N + FR+ 
Sbjct: 110 AEEKKKYLRPVDNIEGYGNDMVFSEQQTLDWTDRLYLTVYPPDKRKLKFWPENPKSFRDT 169

Query: 121 IDEYTTNVKLISEKIFKAMARSLDLDESSFLKQYGEQIKLDARFNFYPRCRNPDLVLGVK 180
           +D+YT+ +++I+E + K MARSL+L+E  FL +YGEQ K+DARFNFYP C  PD VLG K
Sbjct: 170 LDQYTSKLQVITETVLKGMARSLNLEEDCFLNKYGEQRKMDARFNFYPPCSRPDRVLGTK 229

Query: 181 PHADGSAITILLQDKEVEGLQFMKDNEWFNAPIVPDALLVNVGDQVEITSNGIFKSPVHR 240
           PHAD + IT+LLQDKEVEGLQFMKD++W+ APIVP+ALL+NVGDQ EI SNG+FKSPVH+
Sbjct: 230 PHADETLITLLLQDKEVEGLQFMKDDQWYRAPIVPEALLINVGDQAEILSNGLFKSPVHK 289

Query: 241 VLTNSERERISLAVFYLPDAEKEIEPLEELISETQPRLYKSVKNFVGLYFEYYQQGQRPM 300
           V+TN+E+ERISLAVF +PD+E EIEP E LI+E++PRLYK ++N+VG+YFEYYQQG+RP+
Sbjct: 290 VVTNAEKERISLAVFCVPDSETEIEPFESLINESRPRLYKKLRNYVGIYFEYYQQGRRPI 349

Query: 301 EAARI 306
           EAA I
Sbjct: 350 EAAII 354

[Close]
BLAST of Cucsa.350520 vs. NCBI nr
Match: gi|692342230|gb|AIS20826.1| (EFE [Pyrus x bretschneideri])

HSP 1 Score: 425.2 bits (1092), Expect = 9.4e-116
Identity = 196/306 (64.05%), Postives = 255/306 (83.33%), Query Frame = 1 

Query: 1   MAAPVIDIALLSSSSKSGPELEKLRHGLQSWGCFQAINHGMTSEYLDEVRRLTKQFFGLS 60
           M  PVID+ALL++SS S  ELEKLR  L +WGCFQ INHGM+ E+LDE R +TKQFF L 
Sbjct: 43  MDVPVIDLALLAASSISADELEKLRSALATWGCFQVINHGMSPEFLDEFREITKQFFALP 102

Query: 61  MEEKLKYLKEELEMEGYGNDMILSNQQILDWTDRLYLTVYPHQSRRFKYWPTNLQRFREV 120
           +E+K KYL++  +++GYGNDM+ S QQ LDW+DRLYL+VYP  +R+   WP N + FR+ 
Sbjct: 103 VEDKKKYLRQVNDIQGYGNDMVFSEQQTLDWSDRLYLSVYPQDNRKLNLWPENPKSFRQT 162

Query: 121 IDEYTTNVKLISEKIFKAMARSLDLDESSFLKQYG-EQIKLDARFNFYPRCRNPDLVLGV 180
           +D+YT +++++++ + KAMARSLDL+++ F   YG EQ K+D RFNFYP C  PDLVLGV
Sbjct: 163 LDQYTMDLQVVTKTVLKAMARSLDLEDNCFSDLYGGEQGKMDIRFNFYPPCPRPDLVLGV 222

Query: 181 KPHADGSAITILLQDKEVEGLQFMKDNEWFNAPIVPDALLVNVGDQVEITSNGIFKSPVH 240
           KPHADG+ IT+LLQDKEVEGLQF+K ++WF APIVPDALL+NVGDQVEI SNGIFKSPVH
Sbjct: 223 KPHADGTLITLLLQDKEVEGLQFLKGDKWFRAPIVPDALLINVGDQVEILSNGIFKSPVH 282

Query: 241 RVLTNSERERISLAVFYLPDAEKEIEPLEELISETQPRLYKSVKNFVGLYFEYYQQGQRP 300
           +V+TNS++ERIS+A F +P+++KEIEP E L++E++PRLYK VKN+VG+YFEYYQQG+RP
Sbjct: 283 KVVTNSDKERISVAAFCIPESDKEIEPFERLVNESRPRLYKKVKNYVGIYFEYYQQGRRP 342

Query: 301 MEAARI 306
           MEAA++
Sbjct: 343 MEAAKM 348

The following BLAST results are available for this feature:
BLAST of Cucsa.350520 vs. Swiss-Prot
Match NameE-valueIdentityDescription
SRG1_ARATH1.7e-5335.41Protein SRG1 OS=Arabidopsis thaliana GN=SRG1 PE=2 SV=1[more]
DIOX3_PAPSO1.2e-5139.78Codeine O-demethylase OS=Papaver somniferum GN=CODM PE=1 SV=1[more]
DIOX2_PAPSO6.2e-5135.81Probable 2-oxoglutarate/Fe(II)-dependent dioxygenase OS=Papaver somniferum GN=DI... [more]
DIOX1_PAPSO2.9e-4837.76Thebaine 6-O-demethylase OS=Papaver somniferum GN=T6ODM PE=1 SV=1[more]
LDOX_MALDO1.9e-4735.69Leucoanthocyanidin dioxygenase OS=Malus domestica GN=ANS PE=2 SV=1[more]
BLAST of Cucsa.350520 vs. TrEMBL
Match NameE-valueIdentityDescription
A0A0A0LYX6_CUCSA7.9e-17099.02Uncharacterized protein OS=Cucumis sativus GN=Csa_1G263950 PE=3 SV=1[more]
A0A096ZJT4_9ROSA6.5e-11664.05EFE OS=Pyrus x bretschneideri GN=EFE PE=2 SV=1[more]
M5X0W8_PRUPE2.7e-11463.07Uncharacterized protein OS=Prunus persica GN=PRUPE_ppa007944mg PE=3 SV=1[more]
W9RPD3_9ROSA2.3e-11363.61Protein SRG1 OS=Morus notabilis GN=L484_023672 PE=3 SV=1[more]
M5WFY4_PRUPE8.8e-11362.42Uncharacterized protein OS=Prunus persica GN=PRUPE_ppa007960mg PE=3 SV=1[more]
BLAST of Cucsa.350520 vs. TAIR10
Match NameE-valueIdentityDescription
AT5G20400.19.3e-10659.15 2-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily prot... [more]
AT1G49390.18.2e-10256.21 2-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily prot... [more]
AT5G20550.16.7e-9655.37 2-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily prot... [more]
AT5G54000.11.1e-9553.59 2-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily prot... [more]
AT3G21420.19.8e-6340.32 2-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily prot... [more]
BLAST of Cucsa.350520 vs. NCBI nr
Match NameE-valueIdentityDescription
gi|778660150|ref|XP_011655688.1|1.1e-16999.02PREDICTED: protein SRG1-like [Cucumis sativus][more]
gi|659131150|ref|XP_008465535.1|8.4e-16595.08PREDICTED: protein SRG1-like [Cucumis melo][more]
gi|470101600|ref|XP_004287258.1|2.9e-11765.57PREDICTED: protein SRG1-like isoform X1 [Fragaria vesca subsp. vesca][more]
gi|764507132|ref|XP_011457663.1|1.9e-11664.92PREDICTED: protein SRG1-like isoform X2 [Fragaria vesca subsp. vesca][more]
gi|692342230|gb|AIS20826.1|9.4e-11664.05EFE [Pyrus x bretschneideri][more]
Annotated Terms
The following terms have been associated with this gene:
Vocabulary: INTERPRO
TermDefinition
IPR005123Oxoglu/Fe-dep_dioxygenase
IPR026992DIOX_N
IPR027443IPNS-like
Vocabulary: Molecular Function
TermDefinition
GO:0016491oxidoreductase activity
Vocabulary: Biological Process
TermDefinition
GO:0055114oxidation-reduction process
GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0055114 oxidation-reduction process
cellular_component GO:0005575 cellular_component
molecular_function GO:0046872 metal ion binding
molecular_function GO:0016706 oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen, 2-oxoglutarate as one donor, and incorporation of one atom each of oxygen into both donors
molecular_function GO:0016491 oxidoreductase activity
Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Cucsa.350520.1Cucsa.350520.1mRNA


InterPro
Analysis Name: InterPro Annotations of cucumber (Gy14)
Date Performed: 2017-01-17
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR005123Oxoglutarate/iron-dependent dioxygenasePFAMPF031712OG-FeII_Oxycoord: 164..257
score: 1.6
IPR005123Oxoglutarate/iron-dependent dioxygenasePROFILEPS51471FE2OG_OXYcoord: 158..258
score: 12
IPR026992Non-haem dioxygenase N-terminal domainPFAMPF14226DIOX_Ncoord: 4..111
score: 3.6
IPR027443Isopenicillin N synthase-likeGENE3DG3DSA:2.60.120.330coord: 2..283
score: 6.2
NoneNo IPR availablePANTHERPTHR10209OXIDOREDUCTASE, 2OG-FE II OXYGENASE FAMILY PROTEINcoord: 1..299
score: 1.4E
NoneNo IPR availablePANTHERPTHR10209:SF1072-OXOGLUTARATE (2OG) AND FE(II)-DEPENDENT OXYGENASE-LIKE PROTEIN-RELATEDcoord: 1..299
score: 1.4E
NoneNo IPR availableunknownSSF51197Clavaminate synthase-likecoord: 3..283
score: 5.43