Carg27194 (gene) Silver-seed gourd | Cucurbit Genomics Database (CuGenDB)

Overview


Name	Carg27194
Type	gene
Organism	Cucurbita argyrosperma (Silver-seed gourd)
Description	2-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily protein
Location	Cucurbita_argyrosperma_scaffold_486 : 56783 .. 58136 (+)

Genome Viewer

Gene Expression

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideexonCDSthree_prime_UTR

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGACCCAAAAATCTCACCCCCAACAAACACTCCAGCAACAACTTCTCATCAACGGCGGCGATACGCCGGAAAGTTATATTTACAAAGGTGGCTACAGCGGCGGAGATTCCAACAATGATCCACTTCCAATGGCGGAGATTCCGGTCGTTGACCTTTCTCAACTGTCGTCATCGCAAGGCGGCGAGGCTGCTTTAGAGGAGTTCCGGTTGGCTCTTAGTTCTTGGGGCTGTTTTCAGGTCTGGTTTTTGTAAATTTTTTGAATTTTTCTCTCTCTATGGAGCGAATAGATGATTGATGTGTCTGGTGAGGGTTGCAGGCGACTAATCACGGAATTTCGAGTTCATTTTTGGAGAAGATGCGTCAAATAAGTGAGCAATTTTTCGCACTGCCTATGGAGGAGAAGAACAGATACTACAGAGAAATTGATGGACATGAAGGGTATGGGAGTGATTTGATTTTATCGGAGCCCCAAATTCTTGATTGGACGGATCGGTTATATCTGGTCGTGAATCCGGAGGATGAGAGAAAGCTCAAGTTTTGGCCGGAGAATCCTCCATCTTTCAGGTCAGCTTCATTCTAGAAAGCATTTAACACAACATTCATAAACATTAAATGGTTACCCTTTTTTGGTACAGGGAAGATCTACACGAGTTTACAATAAAAGTGAAGGAGATAATTGAAAATGTGCTGATCGCCATGGCAGCATCCTTGAAGGTGGAGCCTAAGAGCTTTTCCGACCAGGTGGGGAAGCGTCCTGTTTTAGTGACGAGGTTCAACTTCTATCCTCCATGTTCGACTCCTCATCTTGTTCTTGGTCTCAAAGAACACTCCGATGGCTCGGCCTTCACCATTGTGTTACTTGACAAAGAAGTGGAAGGCCTGGAAATGAAGAAGGACAACCAGTGGTACAGACTCCCTGTTCCTGCCATCGCTGATTCGCTTCTCATCAACGTTGGGGAACAAGGGGAGGTAAGTTTCAAGTGCAAGTTTTAAGGTTGTCCTTACATTCATGTGTGTGCCTCTTATGTTATTGTGTATCTGTGCAGATTATGAGCAATGGGATATTCAAGAGCCCTGTTCATCGGGCGGTAACAAATTCAGAGAGGCAGAGGATTTCAGTGGCTTGTTTCTGCTGCCCAGAAAAAGATAAGGAGATCAAGCCGATTGAGGGGCTGATCGACGAGAGGAGGCCGAGATTGTTCAGAACTGTGAAGAACTATGTGGAAGAAACTTATTTTCAGTATTACCAGAAGGGGCAGAGAGCAGTTGATGAATTGAAGATTTAAAAAACATCAACACTAATCTCTGTTTTTTGTACTGCTTTCACTGAATGTTTCAATAATAGGCTGTTCT

mRNA sequence

ATGACCCAAAAATCTCACCCCCAACAAACACTCCAGCAACAACTTCTCATCAACGGCGGCGATACGCCGGAAAGTTATATTTACAAAGGTGGCTACAGCGGCGGAGATTCCAACAATGATCCACTTCCAATGGCGGAGATTCCGGTCGTTGACCTTTCTCAACTGTCGTCATCGCAAGGCGGCGAGGCTGCTTTAGAGGAGTTCCGGTTGGCTCTTAGTTCTTGGGGCTGTTTTCAGGCGACTAATCACGGAATTTCGAGTTCATTTTTGGAGAAGATGCGTCAAATAAGTGAGCAATTTTTCGCACTGCCTATGGAGGAGAAGAACAGATACTACAGAGAAATTGATGGACATGAAGGGTATGGGAGTGATTTGATTTTATCGGAGCCCCAAATTCTTGATTGGACGGATCGGTTATATCTGGTCGTGAATCCGGAGGATGAGAGAAAGCTCAAGTTTTGGCCGGAGAATCCTCCATCTTTCAGGGAAGATCTACACGAGTTTACAATAAAAGTGAAGGAGATAATTGAAAATGTGCTGATCGCCATGGCAGCATCCTTGAAGGTGGAGCCTAAGAGCTTTTCCGACCAGGTGGGGAAGCGTCCTGTTTTAGTGACGAGGTTCAACTTCTATCCTCCATGTTCGACTCCTCATCTTGTTCTTGGTCTCAAAGAACACTCCGATGGCTCGGCCTTCACCATTGTGTTACTTGACAAAGAAGTGGAAGGCCTGGAAATGAAGAAGGACAACCAGTGGTACAGACTCCCTGTTCCTGCCATCGCTGATTCGCTTCTCATCAACGTTGGGGAACAAGGGGAGATTATGAGCAATGGGATATTCAAGAGCCCTGTTCATCGGGCGGTAACAAATTCAGAGAGGCAGAGGATTTCAGTGGCTTGTTTCTGCTGCCCAGAAAAAGATAAGGAGATCAAGCCGATTGAGGGGCTGATCGACGAGAGGAGGCCGAGATTGTTCAGAACTGTGAAGAACTATGTGGAAGAAACTTATTTTCAGTATTACCAGAAGGGGCAGAGAGCAGTTGATGAATTGAAGATTTAAAAAACATCAACACTAATCTCTGTTTTTTGTACTGCTTTCACTGAATGTTTCAATAATAGGCTGTTCT

Coding sequence (CDS)

ATGACCCAAAAATCTCACCCCCAACAAACACTCCAGCAACAACTTCTCATCAACGGCGGCGATACGCCGGAAAGTTATATTTACAAAGGTGGCTACAGCGGCGGAGATTCCAACAATGATCCACTTCCAATGGCGGAGATTCCGGTCGTTGACCTTTCTCAACTGTCGTCATCGCAAGGCGGCGAGGCTGCTTTAGAGGAGTTCCGGTTGGCTCTTAGTTCTTGGGGCTGTTTTCAGGCGACTAATCACGGAATTTCGAGTTCATTTTTGGAGAAGATGCGTCAAATAAGTGAGCAATTTTTCGCACTGCCTATGGAGGAGAAGAACAGATACTACAGAGAAATTGATGGACATGAAGGGTATGGGAGTGATTTGATTTTATCGGAGCCCCAAATTCTTGATTGGACGGATCGGTTATATCTGGTCGTGAATCCGGAGGATGAGAGAAAGCTCAAGTTTTGGCCGGAGAATCCTCCATCTTTCAGGGAAGATCTACACGAGTTTACAATAAAAGTGAAGGAGATAATTGAAAATGTGCTGATCGCCATGGCAGCATCCTTGAAGGTGGAGCCTAAGAGCTTTTCCGACCAGGTGGGGAAGCGTCCTGTTTTAGTGACGAGGTTCAACTTCTATCCTCCATGTTCGACTCCTCATCTTGTTCTTGGTCTCAAAGAACACTCCGATGGCTCGGCCTTCACCATTGTGTTACTTGACAAAGAAGTGGAAGGCCTGGAAATGAAGAAGGACAACCAGTGGTACAGACTCCCTGTTCCTGCCATCGCTGATTCGCTTCTCATCAACGTTGGGGAACAAGGGGAGATTATGAGCAATGGGATATTCAAGAGCCCTGTTCATCGGGCGGTAACAAATTCAGAGAGGCAGAGGATTTCAGTGGCTTGTTTCTGCTGCCCAGAAAAAGATAAGGAGATCAAGCCGATTGAGGGGCTGATCGACGAGAGGAGGCCGAGATTGTTCAGAACTGTGAAGAACTATGTGGAAGAAACTTATTTTCAGTATTACCAGAAGGGGCAGAGAGCAGTTGATGAATTGAAGATTTAA

Protein sequence

MTQKSHPQQTLQQQLLINGGDTPESYIYKGGYSGGDSNNDPLPMAEIPVVDLSQLSSSQGGEAALEEFRLALSSWGCFQATNHGISSSFLEKMRQISEQFFALPMEEKNRYYREIDGHEGYGSDLILSEPQILDWTDRLYLVVNPEDERKLKFWPENPPSFREDLHEFTIKVKEIIENVLIAMAASLKVEPKSFSDQVGKRPVLVTRFNFYPPCSTPHLVLGLKEHSDGSAFTIVLLDKEVEGLEMKKDNQWYRLPVPAIADSLLINVGEQGEIMSNGIFKSPVHRAVTNSERQRISVACFCCPEKDKEIKPIEGLIDERRPRLFRTVKNYVEETYFQYYQKGQRAVDELKI

Homology

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BLAST of Carg27194 vs. NCBI nr
Match: XP_008465534.1 (PREDICTED: protein SRG1-like [Cucumis melo])

HSP 1 Score: 563.1 bits (1450), Expect = 6.4e-157
Identity = 275/355 (77.46%), Postives = 314/355 (88.45%), Query Frame = 0


Query: 1   MTQKSH-PQ-QTLQQQLLINGGDTPESYIYKGGYSGGDSNND-PLPMAEIPVVDLSQLSS 60
           M Q S  PQ QTLQQQLLINGG TPESYIYKGGY GGDSNN+ PLP+A+IPV+DLSQLSS
Sbjct: 1   MAQNSQIPQTQTLQQQLLINGGHTPESYIYKGGYHGGDSNNNTPLPLAQIPVLDLSQLSS 60

Query: 61  SQGGEAALEEFRLALSSWGCFQATNHGISSSFLEKMRQISEQFFALPMEEKNRYYREIDG 120
           +  GEA L   RLALS+WGCFQATNHGISSSFLEKMR+ISEQFF+LP+EEK RY RE+DG
Sbjct: 61  ASVGEAPLNHLRLALSTWGCFQATNHGISSSFLEKMRKISEQFFSLPIEEKMRYGREVDG 120

Query: 121 HEGYGSDLILSEPQILDWTDRLYLVVNPEDERKLKFWPENPPSFREDLHEFTIKVKEIIE 180
            EGYG+DLI S  QILDW+DRLYLV NP+DER+L+FWP NPPSFREDLHE+T+K+ +II+
Sbjct: 121 MEGYGNDLIFSNQQILDWSDRLYLVTNPKDERRLEFWPLNPPSFREDLHEYTVKLMKIID 180

Query: 181 NVLIAMAASLKVEPKSFSDQVGKRPVLVTRFNFYPPCSTPHLVLGLKEHSDGSAFTIVLL 240
            VLIAMA SL VEP SF+DQVGKRP L  RFNFYPPCSTPHLVLGLKEHSDG+A T++LL
Sbjct: 181 TVLIAMARSLNVEPNSFTDQVGKRPTLFKRFNFYPPCSTPHLVLGLKEHSDGTAITVLLL 240

Query: 241 DKEVEGLEMKKDNQWYRLPVPAIADSLLINVGEQGEIMSNGIFKSPVHRAVTNSERQRIS 300
           DK+VEGLE++KD+QWYR+PVPA+ADSLLI +GEQ EIMSNGIFKS +HRAVTNSERQRIS
Sbjct: 241 DKQVEGLELRKDDQWYRVPVPAVADSLLIIIGEQAEIMSNGIFKSLIHRAVTNSERQRIS 300

Query: 301 VACFCCPEKDKEIKPIEGLIDERRPRLFRTVKNYVEETYFQYYQKGQRAVDELKI 353
           V  FCCPEKD EIKPIEGLIDE+RPRLFR+ KNY+ +TYFQ YQKG+R+VD L+I
Sbjct: 301 VVSFCCPEKDIEIKPIEGLIDEKRPRLFRSTKNYL-QTYFQNYQKGERSVDGLRI 354

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BLAST of Carg27194 vs. NCBI nr
Match: XP_011655280.1 (PREDICTED: protein SRG1-like [Cucumis sativus])

HSP 1 Score: 560.5 bits (1443), Expect = 4.2e-156
Identity = 269/345 (77.97%), Postives = 303/345 (87.83%), Query Frame = 0


Query: 9   QTLQQQLLINGGDTPESYIYKGGYSGGDSNND-PLPMAEIPVVDLSQLSSSQGGEAALEE 68
           QTLQQQLLINGG TPESYIYKGGY GG SNN+ PLP+AEIPVVDLSQLSS   GE  L +
Sbjct: 11  QTLQQQLLINGGHTPESYIYKGGYHGGGSNNNTPLPLAEIPVVDLSQLSSPSAGEGPLND 70

Query: 69  FRLALSSWGCFQATNHGISSSFLEKMRQISEQFFALPMEEKNRYYREIDGHEGYGSDLIL 128
            RLALS+WGCFQATNH ISSSFLEK+R+ISEQFF+LP+EEK RY RE+DG EGYG+DL  
Sbjct: 71  LRLALSTWGCFQATNHSISSSFLEKLRKISEQFFSLPIEEKMRYGREVDGMEGYGNDLTF 130

Query: 129 SEPQILDWTDRLYLVVNPEDERKLKFWPENPPSFREDLHEFTIKVKEIIENVLIAMAASL 188
           S  Q LDW+DRLY V +PEDER+L  WP NPPSFREDLHE+T+K+ EIIE VLIAMA SL
Sbjct: 131 SNQQTLDWSDRLYFVTSPEDERRLDLWPLNPPSFREDLHEYTVKIMEIIETVLIAMARSL 190

Query: 189 KVEPKSFSDQVGKRPVLVTRFNFYPPCSTPHLVLGLKEHSDGSAFTIVLLDKEVEGLEMK 248
            VEP SF+DQVG+RP L TRFNFYPPCSTPHLVLGLKEHSDGSA TI+LLDK+VEGL+++
Sbjct: 191 NVEPNSFTDQVGERPTLFTRFNFYPPCSTPHLVLGLKEHSDGSAITILLLDKQVEGLQLR 250

Query: 249 KDNQWYRLPVPAIADSLLINVGEQGEIMSNGIFKSPVHRAVTNSERQRISVACFCCPEKD 308
           KD+QWYR+PVPAIADSLL+ +GEQ E+MSNGIFKS VHRAVTNSERQRISV CFCCPEKD
Sbjct: 251 KDDQWYRVPVPAIADSLLLVIGEQAEVMSNGIFKSSVHRAVTNSERQRISVVCFCCPEKD 310

Query: 309 KEIKPIEGLIDERRPRLFRTVKNYVEETYFQYYQKGQRAVDELKI 353
            EIKP+EGLIDE+RPRLFR+VKNY+ ETYFQ YQ+GQR VD L+I
Sbjct: 311 IEIKPVEGLIDEKRPRLFRSVKNYL-ETYFQNYQEGQRTVDGLRI 354

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BLAST of Carg27194 vs. NCBI nr
Match: XP_011655698.1 (PREDICTED: protein SRG1-like [Cucumis sativus])

HSP 1 Score: 558.5 bits (1438), Expect = 1.6e-155
Identity = 268/345 (77.68%), Postives = 304/345 (88.12%), Query Frame = 0


Query: 9   QTLQQQLLINGGDTPESYIYKGGYSGGDSNND-PLPMAEIPVVDLSQLSSSQGGEAALEE 68
           QTLQQQLLINGG TPESYIYKGGY GG SNN+ PLP+AEIPVVDLSQLSS   GE  L +
Sbjct: 11  QTLQQQLLINGGHTPESYIYKGGYHGGGSNNNTPLPLAEIPVVDLSQLSSPSAGEGPLND 70

Query: 69  FRLALSSWGCFQATNHGISSSFLEKMRQISEQFFALPMEEKNRYYREIDGHEGYGSDLIL 128
            RLALS+WGCFQA NH ISSSFLEKMR+ISEQFF+LP+EEK RY RE+DG EGYG+DL  
Sbjct: 71  LRLALSTWGCFQAINHSISSSFLEKMRKISEQFFSLPIEEKMRYGREVDGMEGYGNDLTF 130

Query: 129 SEPQILDWTDRLYLVVNPEDERKLKFWPENPPSFREDLHEFTIKVKEIIENVLIAMAASL 188
           S  Q LDW+DRLY V NPEDER+L+ WP NPPSFREDL E+T+K+ EIIE VLIAMA+SL
Sbjct: 131 SNQQTLDWSDRLYFVTNPEDERRLELWPLNPPSFREDLDEYTVKIMEIIETVLIAMASSL 190

Query: 189 KVEPKSFSDQVGKRPVLVTRFNFYPPCSTPHLVLGLKEHSDGSAFTIVLLDKEVEGLEMK 248
            VEP SF+DQVGKRP L+TRFNFYPPCSTPHLVLGLKEHSDGSA TI+LLDK+VEGL+++
Sbjct: 191 DVEPNSFTDQVGKRPTLLTRFNFYPPCSTPHLVLGLKEHSDGSAITILLLDKQVEGLQLR 250

Query: 249 KDNQWYRLPVPAIADSLLINVGEQGEIMSNGIFKSPVHRAVTNSERQRISVACFCCPEKD 308
           KD+QWYR+PVPAIADSLL+ +GEQ E+MSNGIFKS +HRAVTNSERQRIS+ CFCCPEKD
Sbjct: 251 KDDQWYRVPVPAIADSLLLVIGEQAEVMSNGIFKSSIHRAVTNSERQRISLVCFCCPEKD 310

Query: 309 KEIKPIEGLIDERRPRLFRTVKNYVEETYFQYYQKGQRAVDELKI 353
            EIKPIEGLIDE+RPRLF++VKNY+ ETYFQ YQKG+R VD L+I
Sbjct: 311 IEIKPIEGLIDEKRPRLFKSVKNYL-ETYFQNYQKGRRPVDGLRI 354

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BLAST of Carg27194 vs. NCBI nr
Match: XP_022134811.1 (uncharacterized protein LOC111006992 [Momordica charantia])

HSP 1 Score: 556.6 bits (1433), Expect = 6.0e-155
Identity = 267/344 (77.62%), Postives = 305/344 (88.66%), Query Frame = 0


Query: 9   QTLQQQLLINGGDTPESYIYKGGYSGGDSNNDPLPMAEIPVVDLSQLSSSQGGEAALEEF 68
           +T QQ+LLINGGDTPESYIYK GY GGDSNN+PLP+AEIPVVDL+QLSSS    AALE+ 
Sbjct: 363 KTFQQKLLINGGDTPESYIYKDGYGGGDSNNNPLPLAEIPVVDLAQLSSSPPSTAALEDL 422

Query: 69  RLALSSWGCFQATNHGISSSFLEKMRQISEQFFALPMEEKNRYYREIDGHEGYGSDLILS 128
           RLAL+SWGCFQA NH ISSSFL K+ QIS QFF+LPMEEKN+  RE+ G EGYG+D++ S
Sbjct: 423 RLALTSWGCFQAINHSISSSFLNKIHQISNQFFSLPMEEKNKCCRELYGLEGYGTDMVFS 482

Query: 129 EPQILDWTDRLYLVVNPEDERKLKFWPENPPSFREDLHEFTIKVKEIIENVLIAMAASLK 188
           E QILDWTDRLYL VNPEDER+LK+WP+NP SFREDLHEFTIK+K+IIE VL+AMA S+ 
Sbjct: 483 EQQILDWTDRLYLKVNPEDERQLKYWPQNPQSFREDLHEFTIKLKQIIETVLMAMARSIN 542

Query: 189 VEPKSFSDQVGKRPVLVTRFNFYPPCSTPHLVLGLKEHSDGSAFTIVLLDKEVEGLEMKK 248
           VE  SF++QVGKRP L TRFNFYPPCS P LVLGLKEHSDGSA TIVLLD+EVEGL+ +K
Sbjct: 543 VEANSFTEQVGKRPELFTRFNFYPPCSRPDLVLGLKEHSDGSAITIVLLDREVEGLQWRK 602

Query: 249 DNQWYRLPVPAIADSLLINVGEQGEIMSNGIFKSPVHRAVTNSERQRISVACFCCPEKDK 308
           D+QW+R+PVPA+ADSLLIN+GEQ EIMSNG+FKS VHRAVTNSE+QRISVACFCCPEKD+
Sbjct: 603 DDQWFRVPVPAMADSLLINIGEQAEIMSNGVFKSAVHRAVTNSEKQRISVACFCCPEKDR 662

Query: 309 EIKPIEGLIDERRPRLFRTVKNYVEETYFQYYQKGQRAVDELKI 353
           EI+PIEGLIDERRPRL+R VKNYV  +YFQ YQKGQR VD+LKI
Sbjct: 663 EIEPIEGLIDERRPRLYRNVKNYV-GSYFQDYQKGQRPVDKLKI 705

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BLAST of Carg27194 vs. NCBI nr
Match: KGN65097.1 (hypothetical protein Csa_1G212830 [Cucumis sativus])

HSP 1 Score: 481.9 bits (1239), Expect = 1.9e-132
Identity = 227/298 (76.17%), Postives = 259/298 (86.91%), Query Frame = 0


Query: 55  LSSSQGGEAALEEFRLALSSWGCFQATNHGISSSFLEKMRQISEQFFALPMEEKNRYYRE 114
           L S   GE  L + RLALS+WGCFQATNH ISSSFLEK+R+ISEQFF+LP+EEK RY RE
Sbjct: 281 LKSPSAGEGPLNDLRLALSTWGCFQATNHSISSSFLEKLRKISEQFFSLPIEEKMRYGRE 340

Query: 115 IDGHEGYGSDLILSEPQILDWTDRLYLVVNPEDERKLKFWPENPPSFREDLHEFTIKVKE 174
           +DG EGYG+DL  S  Q LDW+DRLY V +PEDER+L  WP NPPSFREDLHE+T+K+ E
Sbjct: 341 VDGMEGYGNDLTFSNQQTLDWSDRLYFVTSPEDERRLDLWPLNPPSFREDLHEYTVKIME 400

Query: 175 IIENVLIAMAASLKVEPKSFSDQVGKRPVLVTRFNFYPPCSTPHLVLGLKEHSDGSAFTI 234
           IIE VLIAMA SL VEP SF+DQVG+RP L TRFNFYPPCSTPHLVLGLKEHSDGSA TI
Sbjct: 401 IIETVLIAMARSLNVEPNSFTDQVGERPTLFTRFNFYPPCSTPHLVLGLKEHSDGSAITI 460

Query: 235 VLLDKEVEGLEMKKDNQWYRLPVPAIADSLLINVGEQGEIMSNGIFKSPVHRAVTNSERQ 294
           +LLDK+VEGL+++KD+QWYR+PVPAIADSLL+ +GEQ E+MSNGIFKS VHRAVTNSERQ
Sbjct: 461 LLLDKQVEGLQLRKDDQWYRVPVPAIADSLLLVIGEQAEVMSNGIFKSSVHRAVTNSERQ 520

Query: 295 RISVACFCCPEKDKEIKPIEGLIDERRPRLFRTVKNYVEETYFQYYQKGQRAVDELKI 353
           RISV CFCCPEKD EIKP+EGLIDE+RPRLFR+VKNY+ ETYFQ YQ+GQR VD L+I
Sbjct: 521 RISVVCFCCPEKDIEIKPVEGLIDEKRPRLFRSVKNYL-ETYFQNYQEGQRTVDGLRI 577

[Close]

BLAST of Carg27194 vs. TAIR10
Match: AT5G20400.1 (2-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily protein)

HSP 1 Score: 359.8 bits (922), Expect = 2.0e-99
Identity = 176/347 (50.72%), Postives = 243/347 (70.03%), Query Frame = 0


Query: 7   PQQTLQQQLLINGGDTPESYIYKGGYSGGDSNNDPL----PMAEIPVVDLS-QLSSSQGG 66
           P+    Q+++  G   PE Y++      GD    PL    P  +IP +DL+  LSSS+ G
Sbjct: 4   PKFKTVQEVVAAGEGLPERYLHA---PTGDGEVQPLNAAVPEMDIPAIDLNLLLSSSEAG 63

Query: 67  EAALEEFRLALSSWGCFQATNHGISSSFLEKMRQISEQFFALPMEEKNRYYREIDGHEGY 126
           +  L +   ALS+WG  Q  NHGI+ +FL+K+ +++++FFALP EEK +  REID  +GY
Sbjct: 64  QQELSKLHSALSTWGVVQVMNHGITKAFLDKIYKLTKEFFALPTEEKQKCAREIDSIQGY 123

Query: 127 GSDLILSEPQILDWTDRLYLVVNPEDERKLKFWPENPPSFREDLHEFTIKVKEIIENVLI 186
           G+D+IL + Q+LDW DRLY+   PED+R+L FWPE P  FRE LHE+T+K + +IE    
Sbjct: 124 GNDMILWDDQVLDWIDRLYITTYPEDQRQLNFWPEVPLGFRETLHEYTMKQRIVIEQFFK 183

Query: 187 AMAASLKVEPKSFSDQVGKRPVLVTRFNFYPPCSTPHLVLGLKEHSDGSAFTIVLLDKEV 246
           AMA SL++E  SF D  G+   L TRFN YPPC +P  V+G+K H+DGSA T++L DK+V
Sbjct: 184 AMARSLELEENSFLDMYGESATLDTRFNMYPPCPSPDKVIGVKPHADGSAITLLLPDKDV 243

Query: 247 EGLEMKKDNQWYRLPVPAIADSLLINVGEQGEIMSNGIFKSPVHRAVTNSERQRISVACF 306
            GL+ +KD +WY+ P+  + D++LINVG+Q EIMSNGI+KSPVHR VTN E++RISVA F
Sbjct: 244 GGLQFQKDGKWYKAPI--VPDTILINVGDQMEIMSNGIYKSPVHRVVTNREKERISVATF 303

Query: 307 CCPEKDKEIKPIEGLIDERRPRLFRTVKNYVEETYFQYYQKGQRAVD 349
           C P  DKEI+P+  L+ E RPRL++TVK YV E YF+YYQ+G+R ++
Sbjct: 304 CIPGADKEIQPVNELVSEARPRLYKTVKKYV-ELYFKYYQQGRRPIE 344

[Close]

BLAST of Carg27194 vs. TAIR10
Match: AT5G54000.1 (2-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily protein)

HSP 1 Score: 349.4 bits (895), Expect = 2.7e-96
Identity = 168/349 (48.14%), Postives = 236/349 (67.62%), Query Frame = 0


Query: 7   PQQTLQQQLLINGGDTPESYIY--KGGYSGGDSNNDPLPMAEIPVVDLSQL-SSSQGGEA 66
           P+    Q+++  G   PE Y+Y   G   G    N  LP  +I ++DL+ L SSS  G  
Sbjct: 4   PKLKTVQEVVAAGEKLPERYLYTPTGDGEGDQPFNGLLPEMKISIIDLNLLFSSSDDGRE 63

Query: 67  ALEEFRLALSSWGCFQATNHGISSSFLEKMRQISEQFFALPMEEKNRYYREIDGHEGYGS 126
            L +   A+S+WG  Q  NHGIS + L+K+ ++++QFF LP +EK +Y REI   +G+G+
Sbjct: 64  ELSKLHSAISTWGVVQVMNHGISEALLDKIHELTKQFFVLPTKEKQKYAREISSFQGFGN 123

Query: 127 DLILSEPQILDWTDRLYLVVNPEDERKLKFWPENPPSFREDLHEFTIKVKEIIENVLIAM 186
           D+ILS+ Q+LDW DRLYL+  PED+R+LKFWPENP  FRE LHE+T+K + ++E    A+
Sbjct: 124 DMILSDDQVLDWVDRLYLITYPEDQRQLKFWPENPSGFRETLHEYTMKQQLVVEKFFKAL 183

Query: 187 AASLKVEPKSFSDQVGKRPVLVTRFNFYPPCSTPHLVLGLKEHSDGSAFTIVLLDKEVEG 246
           A SL++E   F +  G+   L TRFN YPPC  P  VLGLK HSDGSAFT++L DK VEG
Sbjct: 184 ARSLELEDNCFLEMHGENATLETRFNIYPPCPRPDKVLGLKPHSDGSAFTLILPDKNVEG 243

Query: 247 LEMKKDNQWYRLPVPAIADSLLINVGEQGEIMSNGIFKSPVHRAVTNSERQRISVACFCC 306
           L+  KD +WY+  +  +  ++LINVG+  E+MSNGI+KSPVHR V N +++RI VA FC 
Sbjct: 244 LQFLKDGKWYKASI--LPHTILINVGDTMEVMSNGIYKSPVHRVVLNGKKERIYVATFCN 303

Query: 307 PEKDKEIKPIEGLIDERRPRLFRTVKNYVEETYFQYYQKGQRAVDELKI 353
            ++DKEI+P+ GL+ E RPRL++ VK   E+ +F YYQ+G+R ++   I
Sbjct: 304 ADEDKEIQPLNGLVSEARPRLYKAVKK-SEKNFFDYYQQGRRPIEAAMI 349

[Close]

BLAST of Carg27194 vs. TAIR10
Match: AT5G20550.1 (2-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily protein)

HSP 1 Score: 343.6 bits (880), Expect = 1.5e-94
Identity = 169/343 (49.27%), Postives = 233/343 (67.93%), Query Frame = 0


Query: 7   PQQTLQQQLLINGGDTPESYIYKGGY-SGGDSNNDPLPMAEIPVVDLS-QLSSSQGGEAA 66
           P+    Q+++  G   PE Y+        G   N  +P+ +IP +DLS  LS S  G   
Sbjct: 4   PKFKTVQEVVAAGEGIPERYLQPPAVDDNGQHLNAAVPVMDIPAIDLSLLLSPSDDGREE 63

Query: 67  LEEFRLALSSWGCFQATNHGISSSFLEKMRQISEQFFALPMEEKNRYYREIDGHEGYGSD 126
           L +   ALS+WG  Q  NHGI+ + L+K+ +++++F ALP EEK +Y REI   +GYG+D
Sbjct: 64  LSKLHSALSTWGVVQVINHGITKALLDKIYKLTKEFCALPSEEKQKYAREIGSIQGYGND 123

Query: 127 LILSEPQILDWTDRLYLVVNPEDERKLKFWPENPPSFREDLHEFTIKVKEIIENVLIAMA 186
           +IL + Q+LDW DRLY+   PED+R+LKFWP+ P  FRE LHE+T+K   +   V  AMA
Sbjct: 124 MILWDDQVLDWIDRLYITTYPEDQRQLKFWPDVPVGFRETLHEYTMKQHLVFNQVFKAMA 183

Query: 187 ASLKVEPKSFSDQVGKRPVLVTRFNFYPPCSTPHLVLGLKEHSDGSAFTIVLLDKEVEGL 246
            SL++E   F D  G+   + TRFN YPPC  P  V+G++ H+D SAFT++L DK VEGL
Sbjct: 184 ISLELEENCFLDMCGENATMDTRFNMYPPCPRPDKVIGVRPHADKSAFTLLLPDKNVEGL 243

Query: 247 EMKKDNQWYRLPVPAIADSLLINVGEQGEIMSNGIFKSPVHRAVTNSERQRISVACFCCP 306
           +  KD +WY+ PV A +D++LINVG+Q EIMSNGI+KSPVHR VTN+E++RISVA FC P
Sbjct: 244 QFLKDGKWYKAPVVA-SDTILINVGDQMEIMSNGIYKSPVHRVVTNTEKERISVATFCIP 303

Query: 307 EKDKEIKPIEGLIDERRPRLFRTVKNYVEETYFQYYQKGQRAV 348
             DKEI+P++GL+ E RPRL++ VKNYV +   +YY +GQR +
Sbjct: 304 GADKEIQPVDGLVSEARPRLYKPVKNYV-DLLNKYYIQGQRPI 344

[Close]

BLAST of Carg27194 vs. TAIR10
Match: AT1G49390.1 (2-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily protein)

HSP 1 Score: 341.7 bits (875), Expect = 5.5e-94
Identity = 165/344 (47.97%), Postives = 239/344 (69.48%), Query Frame = 0


Query: 7   PQQTLQQQLLINGGDTPESYIY-KGGYSGGDSNNDPLPMAEIPVVDLSQL-SSSQGGEAA 66
           P+    Q+++  G   PE Y++   G       N  +P  +IP +DLS L SSS  G+  
Sbjct: 4   PKFKTVQEVVAAGQGLPERYLHAPTGEGESQPLNGAVPEMDIPAIDLSLLFSSSVDGQEE 63

Query: 67  LEEFRLALSSWGCFQATNHGISSSFLEKMRQISEQFFALPMEEKNRYYREIDGHEGYGSD 126
           +++   ALS+WG  Q  NHGI+ +FL+K+ ++++QFFALP EEK++  RE    +GYG+D
Sbjct: 64  MKKLHSALSTWGVVQVMNHGITEAFLDKIYKLTKQFFALPTEEKHKCARETGNIQGYGND 123

Query: 127 LILSEPQILDWTDRLYLVVNPEDERKLKFWPENPPSFREDLHEFTIKVKEIIENVLIAMA 186
           +ILS+ Q+LDW DRL+L   PED+R+LKFWP+ P  F E L E+T+K + +IE    AMA
Sbjct: 124 MILSDNQVLDWIDRLFLTTYPEDKRQLKFWPQVPVGFSETLDEYTMKQRVLIEKFFKAMA 183

Query: 187 ASLKVEPKSFSDQVGKRPVLVTRFNFYPPCSTPHLVLGLKEHSDGSAFTIVLLDKEVEGL 246
            SL++E   F +  G+  V+ +RFNF+PPC  P  V+G+K H+DGSA T++L DK+VEGL
Sbjct: 184 RSLELEENCFLEMYGENAVMNSRFNFFPPCPRPDKVIGIKPHADGSAITLLLPDKDVEGL 243

Query: 247 EMKKDNQWYRLPVPAIADSLLINVGEQGEIMSNGIFKSPVHRAVTNSERQRISVACFCCP 306
           +  KD +WY+ P+  + D++LI +G+Q EIMSNGI+KSPVHR VTN E++RISVA FC P
Sbjct: 244 QFLKDGKWYKAPI--VPDTILITLGDQMEIMSNGIYKSPVHRVVTNREKERISVATFCVP 303

Query: 307 EKDKEIKPIEGLIDERRPRLFRTVKNYVEETYFQYYQKGQRAVD 349
             DKEI P +GL+ E RPRL++TV  YV + +++YYQ+G+R ++
Sbjct: 304 GLDKEIHPADGLVTEARPRLYKTVTKYV-DLHYKYYQQGRRTIE 344

[Close]

BLAST of Carg27194 vs. TAIR10
Match: AT3G21420.1 (2-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily protein)

HSP 1 Score: 224.9 bits (572), Expect = 7.5e-59
Identity = 121/313 (38.66%), Postives = 186/313 (59.42%), Query Frame = 0


Query: 46  EIPVVDLSQLSSSQGGEAALEEFRL--ALSSWGCFQATNHGISSSFLEKMRQISEQFFAL 105
           +IPV+DLS+LS     +   E  +L  A   WG FQ  NHGI    +E + +++ +FF +
Sbjct: 54  QIPVIDLSKLSKPDNDDFFFEILKLSQACEDWGFFQVINHGIEVEVVEDIEEVASEFFDM 113

Query: 106 PMEEKNRYYREIDGHEGYGSDLILSEPQILDWTDRLYLVVNPEDERKLKFWPENPPSFRE 165
           P+EEK +Y  E    +GYG   I SE Q LDW +   L V+P   R  K WP  P  F E
Sbjct: 114 PLEEKKKYPMEPGTVQGYGQAFIFSEDQKLDWCNMFALGVHPPQIRNPKLWPSKPARFSE 173

Query: 166 DLHEFTIKVKEIIENVLIAMAASLKVEPKSFSDQVGKRPVLVTRFNFYPPCSTPHLVLGL 225
            L  ++ +++E+ + +L  +A SL ++ + F +  G+  V   R N+YPPCS+P LVLGL
Sbjct: 174 SLEGYSKEIRELCKRLLKYIAISLGLKEERFEEMFGE-AVQAVRMNYYPPCSSPDLVLGL 233

Query: 226 KEHSDGSAFTIVLLDK-EVEGLEMKKDNQWYRLPVPAIADSLLINVGEQGEIMSNGIFKS 285
             HSDGSA T++   K    GL++ KDN W  +PV  + ++L+IN+G+  E++SNG +KS
Sbjct: 234 SPHSDGSALTVLQQSKNSCVGLQILKDNTW--VPVKPLPNALVINIGDTIEVLSNGKYKS 293

Query: 286 PVHRAVTNSERQRISVACFCCPEKDKEIKPIEGLI-DERRPRLFRTVKNYVEETYFQYYQ 345
             HRAVTN E++R+++  F  P  + EI+P+  L+ DE  P  +R+  N+ + +Y     
Sbjct: 294 VEHRAVTNREKERLTIVTFYAPNYEVEIEPMSELVDDETNPCKYRSY-NHGDYSYHYVSN 353

Query: 346 K--GQRAVDELKI 353
           K  G++++D  KI
Sbjct: 354 KLQGKKSLDFAKI 362

[Close]

BLAST of Carg27194 vs. Swiss-Prot
Match: sp|Q39224|SRG1_ARATH (Protein SRG1 OS=Arabidopsis thaliana OX=3702 GN=SRG1 PE=2 SV=1)

HSP 1 Score: 218.8 bits (556), Expect = 9.8e-56
Identity = 112/312 (35.90%), Postives = 186/312 (59.62%), Query Frame = 0


Query: 46  EIPVVDLSQLSSSQGGEAALEEFRLALSSWGCFQATNHGISSSFLEKMRQISEQFFALPM 105
           EIP++D+ +L SS   ++ +E+   A   WG FQ  NHGI SSFL+K++   + FF LPM
Sbjct: 52  EIPIIDMKRLCSSTTMDSEVEKLDFACKEWGFFQLVNHGIDSSFLDKVKSEIQDFFNLPM 111

Query: 106 EEKNRYYREIDGHEGYGSDLILSEPQILDWTDRLYLVVNPEDERKLKFWPENPPSFREDL 165
           EEK ++++  D  EG+G   ++SE Q LDW D  +  V P + RK   +P+ P  FR+ L
Sbjct: 112 EEKKKFWQRPDEIEGFGQAFVVSEDQKLDWADLFFHTVQPVELRKPHLFPKLPLPFRDTL 171

Query: 166 HEFTIKVKEIIENVLIAMAASLKVEPKSFS---DQVGKRPVLVTRFNFYPPCSTPHLVLG 225
             ++ +V+ + + ++  MA +L+++P+      D V    V   R N+YPPC  P  V+G
Sbjct: 172 EMYSSEVQSVAKILIAKMARALEIKPEELEKLFDDVDS--VQSMRMNYYPPCPQPDQVIG 231

Query: 226 LKEHSDGSAFTIVLLDKEVEGLEMKKDNQWYRLPVPAIADSLLINVGEQGEIMSNGIFKS 285
           L  HSD    T+++   +VEGL++KKD +W  +PV  + ++ ++N+G+  EI++NG ++S
Sbjct: 232 LTPHSDSVGLTVLMQVNDVEGLQIKKDGKW--VPVKPLPNAFIVNIGDVLEIITNGTYRS 291

Query: 286 PVHRAVTNSERQRISVACFCCPEKDKEIKPIEGLIDERRPRLFR--TVKNYVEETYFQYY 345
             HR V NSE++R+S+A F      KE+ P + L++ ++   F+  T+K Y  +  F   
Sbjct: 292 IEHRGVVNSEKERLSIATFHNVGMYKEVGPAKSLVERQKVARFKRLTMKEY-NDGLFSRT 351

Query: 346 QKGQRAVDELKI 353
             G+  +D L+I
Sbjct: 352 LDGKAYLDALRI 358

[Close]

BLAST of Carg27194 vs. Swiss-Prot
Match: sp|A2A1A0|NCS1_COPJA (S-norcoclaurine synthase 1 OS=Coptis japonica OX=3442 GN=NCS1 PE=1 SV=1)

HSP 1 Score: 208.0 bits (528), Expect = 1.7e-52
Identity = 106/274 (38.69%), Postives = 162/274 (59.12%), Query Frame = 0


Query: 46  EIPVVDLSQLSSSQGGEAALEEFRLALSSWGCFQATNHGISSSFLEKMRQISEQFFALPM 105
           EIPV+DLS+L   Q     L +F  A   WG FQ  NHG+    +EKM+  +E FF LP 
Sbjct: 50  EIPVIDLSRLLDQQYACDELAKFHSACLDWGFFQLINHGVREEVIEKMKVDTEDFFRLPF 109

Query: 106 EEKNRYYREIDGHEGYGSDLILSEPQILDWTDRLYLVVNPEDERKLKFWPENPPSFREDL 165
           +EKN Y +  +G EGYG   + SE Q LDW D  +L+  P  ER ++FWP +P SFRE +
Sbjct: 110 KEKNAYRQLPNGMEGYGQAFVTSEEQKLDWADMHFLITKPVQERNMRFWPTSPTSFRETM 169

Query: 166 HEFTIKVKEIIENVLIAMAASLKVEPKSFSDQVGKRPVLVTRFNFYPPCSTPHLVLGLKE 225
            +++++++++   +   MA +L +E +  +  +  R V        P  S+    LGL  
Sbjct: 170 EKYSMELQKVAMCLTGMMAKNLGLESEILTKPL--RTVFNREDELLPSMSSCGEGLGLSP 229

Query: 226 HSDGSAFTIVLLDKEVEGLEMKKDNQWYRLPVPAIADSLLINVGEQGEIMSNGIFKSPVH 285
           HSD +  T+++   EV GL +KKD +W  +P+  I  + ++N+G+  EIMSNGI+KS  H
Sbjct: 230 HSDATGLTLLIQVNEVNGLHIKKDEKW--VPIKPILGAFVVNIGDVIEIMSNGIYKSIEH 289

Query: 286 RAVTNSERQRISVACFCCPEKDKEIKPIEGLIDE 320
           RAV N++++R+S+A F  PE   +I P+  L+ E
Sbjct: 290 RAVINTDKERLSIAAFHDPEYGTKIGPLPDLVKE 319

[Close]

BLAST of Carg27194 vs. Swiss-Prot
Match: sp|O80449|DIOX4_ARATH (Probable 2-oxoglutarate-dependent dioxygenase ANS OS=Arabidopsis thaliana OX=3702 GN=ANS PE=2 SV=1)

HSP 1 Score: 206.5 bits (524), Expect = 5.0e-52
Identity = 102/286 (35.66%), Postives = 165/286 (57.69%), Query Frame = 0


Query: 46  EIPVVDLSQLSSSQGGEAALEEFRLALSSWGCFQATNHGISSSFLEKMRQISEQFFALPM 105
           EIPV+D++ +    G    L   R A   WG FQ  NHG++ S +E++R    +FF LP+
Sbjct: 47  EIPVLDMNDV---WGKPEGLRLVRSACEEWGFFQMVNHGVTHSLMERVRGAWREFFELPL 106

Query: 106 EEKNRYYREIDGHEGYGSDLILSEPQILDWTDRLYLVVNPEDERKLKFWPENPPSFREDL 165
           EEK +Y    D +EGYGS L + +   LDW+D  +L   P   R    WP  PP  RE +
Sbjct: 107 EEKRKYANSPDTYEGYGSRLGVVKDAKLDWSDYFFLNYLPSSIRNPSKWPSQPPKIRELI 166

Query: 166 HEFTIKVKEIIENVLIAMAASLKVEPKSFSDQV--GKRPVLVTRFNFYPPCSTPHLVLGL 225
            ++  +V+++ E +   ++ SL ++P      +  G +     R NFYP C  P L LGL
Sbjct: 167 EKYGEEVRKLCERLTETLSESLGLKPNKLMQALGGGDKVGASLRTNFYPKCPQPQLTLGL 226

Query: 226 KEHSDGSAFTIVLLDKEVEGLEMKKDNQWYRLPVPAIADSLLINVGEQGEIMSNGIFKSP 285
             HSD    TI+L D++V GL++++ + W  + + ++ ++L++N+G+Q +I+SNGI+KS 
Sbjct: 227 SSHSDPGGITILLPDEKVAGLQVRRGDGW--VTIKSVPNALIVNIGDQLQILSNGIYKSV 286

Query: 286 VHRAVTNSERQRISVACFCCPEKDKEIKPIEGLIDERRPRLFRTVK 330
            H+ + NS  +R+S+A F  P  D  + PIE L+   RP L++ ++
Sbjct: 287 EHQVIVNSGMERVSLAFFYNPRSDIPVGPIEELVTANRPALYKPIR 327

[Close]

BLAST of Carg27194 vs. Swiss-Prot
Match: sp|D4N502|DIOX3_PAPSO (Codeine O-demethylase OS=Papaver somniferum OX=3469 GN=CODM PE=1 SV=1)

HSP 1 Score: 201.8 bits (512), Expect = 1.2e-50
Identity = 109/283 (38.52%), Postives = 165/283 (58.30%), Query Frame = 0


Query: 47  IPVVDLSQLSSSQG--GEAALEEFRLALSSWGCFQATNHGISSSFLEKMRQISEQFFALP 106
           +PV+DL  L S +   G+  L++   A   WG FQ  NHG+ +  ++ ++   + FF LP
Sbjct: 54  VPVIDLQNLLSPEPVVGKLELDKLHSACKEWGFFQLVNHGVDALLMDNIKSEIKGFFNLP 113

Query: 107 MEEKNRYYREIDGHEGYGSDLILSEPQILDWTDRLYLVVNPEDERKLKFWPENPPSFRED 166
           M EK +Y ++    EG+G   I SE Q LDWT+   ++  P   RK   +PE P  FRE 
Sbjct: 114 MNEKTKYGQQDGDFEGFGQPYIESEDQRLDWTEVFSMLSLPLHLRKPHLFPELPLPFRET 173

Query: 167 LHEFTIKVKEIIENVLIAMAASLK-VEPKSFSDQVGKRPVLVTRFNFYPPCSTPHLVLGL 226
           L  +  K+K++   V   +  SL+ VE K  +D + +  +   R N+YPPC  P LVLGL
Sbjct: 174 LESYLSKMKKLSTVVFEMLEKSLQLVEIKGMTD-LFEDGLQTMRMNYYPPCPRPELVLGL 233

Query: 227 KEHSDGSAFTIVLLDKEVEGLEMKKDNQWYRLPVPAIADSLLINVGEQGEIMSNGIFKSP 286
             HSD S  TI+L   EVEGL+++K+ +W  + +  + D+ ++NVG+  EIM+NGI++S 
Sbjct: 234 TSHSDFSGLTILLQLNEVEGLQIRKEERW--ISIKPLPDAFIVNVGDILEIMTNGIYRSV 293

Query: 287 VHRAVTNSERQRISVACFCCPEKDKEIKPIEGLIDERRPRLFR 327
            HRAV NS ++R+S+A F   + + EI PI  L+    P LF+
Sbjct: 294 EHRAVVNSTKERLSIATFHDSKLESEIGPISSLVTPETPALFK 333

[Close]

BLAST of Carg27194 vs. Swiss-Prot
Match: sp|D4N501|DIOX2_PAPSO (Probable 2-oxoglutarate/Fe(II)-dependent dioxygenase OS=Papaver somniferum OX=3469 GN=DIOX2 PE=2 SV=1)

HSP 1 Score: 193.7 bits (491), Expect = 3.4e-48
Identity = 108/312 (34.62%), Postives = 171/312 (54.81%), Query Frame = 0


Query: 47  IPVVDLSQLSSSQGGEAALEEFRL--ALSSWGCFQATNHGISSSFLEKMRQISEQFFALP 106
           +PV+D+  L SS+     LE  RL  A   WG FQ  NHG+ +S ++ ++   + FF L 
Sbjct: 55  VPVIDIENLISSEPVTEKLELDRLHSACKEWGFFQVVNHGVDTSLVDNVKSDIQGFFNLS 114

Query: 107 MEEKNRYYREIDGHEGYGSDLILSEPQILDWTDRLYLVVNPEDERKLKFWPENPPSFRED 166
           M EK +Y ++    EG+G   + SE Q LDW D   ++  P   RK   + + P   RE 
Sbjct: 115 MNEKIKYGQKDGDVEGFGQAFVASEDQTLDWADIFMILTLPLHLRKPHLFSKLPLPLRET 174

Query: 167 LHEFTIKVKEIIENVLIAMAASLKVEPKSFSD--QVGKRPVLVTRFNFYPPCSTPHLVLG 226
           +  ++ ++K++   +   M  +L+V+     +  +V K    V R N+YPPC  P L +G
Sbjct: 175 IESYSSEMKKLSMVLFEKMEKALQVQAVEIKEISEVFKDMTQVMRMNYYPPCPQPELAIG 234

Query: 227 LKEHSDGSAFTIVLLDKEVEGLEMKKDNQWYRLPVPAIADSLLINVGEQGEIMSNGIFKS 286
           L  HSD    TI+L   EVEGL++K + +W  + V  + ++ ++NVG+  EIM+NG+++S
Sbjct: 235 LTPHSDFGGLTILLQLNEVEGLQIKNEGRW--ISVKPLPNAFVVNVGDVLEIMTNGMYRS 294

Query: 287 PVHRAVTNSERQRISVACFCCPEKDKEIKPIEGLIDERRPRLFRTVKNYVE--ETYFQYY 346
             HRAV NS ++R+S+A F  P  + EI PI  LI    P LFR+   Y E  E +    
Sbjct: 295 VDHRAVVNSTKERLSIATFHDPNLESEIGPISSLITPNTPALFRSGSTYGELVEEFHSRK 354

Query: 347 QKGQRAVDELKI 353
             G+  +D +++
Sbjct: 355 LDGKSFLDSMRM 364

[Close]

BLAST of Carg27194 vs. TrEMBL
Match: tr|A0A1S3CP07|A0A1S3CP07_CUCME (protein SRG1-like OS=Cucumis melo OX=3656 GN=LOC103503159 PE=3 SV=1)

HSP 1 Score: 563.1 bits (1450), Expect = 4.3e-157
Identity = 275/355 (77.46%), Postives = 314/355 (88.45%), Query Frame = 0


Query: 1   MTQKSH-PQ-QTLQQQLLINGGDTPESYIYKGGYSGGDSNND-PLPMAEIPVVDLSQLSS 60
           M Q S  PQ QTLQQQLLINGG TPESYIYKGGY GGDSNN+ PLP+A+IPV+DLSQLSS
Sbjct: 1   MAQNSQIPQTQTLQQQLLINGGHTPESYIYKGGYHGGDSNNNTPLPLAQIPVLDLSQLSS 60

Query: 61  SQGGEAALEEFRLALSSWGCFQATNHGISSSFLEKMRQISEQFFALPMEEKNRYYREIDG 120
           +  GEA L   RLALS+WGCFQATNHGISSSFLEKMR+ISEQFF+LP+EEK RY RE+DG
Sbjct: 61  ASVGEAPLNHLRLALSTWGCFQATNHGISSSFLEKMRKISEQFFSLPIEEKMRYGREVDG 120

Query: 121 HEGYGSDLILSEPQILDWTDRLYLVVNPEDERKLKFWPENPPSFREDLHEFTIKVKEIIE 180
            EGYG+DLI S  QILDW+DRLYLV NP+DER+L+FWP NPPSFREDLHE+T+K+ +II+
Sbjct: 121 MEGYGNDLIFSNQQILDWSDRLYLVTNPKDERRLEFWPLNPPSFREDLHEYTVKLMKIID 180

Query: 181 NVLIAMAASLKVEPKSFSDQVGKRPVLVTRFNFYPPCSTPHLVLGLKEHSDGSAFTIVLL 240
            VLIAMA SL VEP SF+DQVGKRP L  RFNFYPPCSTPHLVLGLKEHSDG+A T++LL
Sbjct: 181 TVLIAMARSLNVEPNSFTDQVGKRPTLFKRFNFYPPCSTPHLVLGLKEHSDGTAITVLLL 240

Query: 241 DKEVEGLEMKKDNQWYRLPVPAIADSLLINVGEQGEIMSNGIFKSPVHRAVTNSERQRIS 300
           DK+VEGLE++KD+QWYR+PVPA+ADSLLI +GEQ EIMSNGIFKS +HRAVTNSERQRIS
Sbjct: 241 DKQVEGLELRKDDQWYRVPVPAVADSLLIIIGEQAEIMSNGIFKSLIHRAVTNSERQRIS 300

Query: 301 VACFCCPEKDKEIKPIEGLIDERRPRLFRTVKNYVEETYFQYYQKGQRAVDELKI 353
           V  FCCPEKD EIKPIEGLIDE+RPRLFR+ KNY+ +TYFQ YQKG+R+VD L+I
Sbjct: 301 VVSFCCPEKDIEIKPIEGLIDEKRPRLFRSTKNYL-QTYFQNYQKGERSVDGLRI 354

[Close]

BLAST of Carg27194 vs. TrEMBL
Match: tr|A0A0A0LW96|A0A0A0LW96_CUCSA (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_1G212830 PE=3 SV=1)

HSP 1 Score: 481.9 bits (1239), Expect = 1.2e-132
Identity = 227/298 (76.17%), Postives = 259/298 (86.91%), Query Frame = 0


Query: 55  LSSSQGGEAALEEFRLALSSWGCFQATNHGISSSFLEKMRQISEQFFALPMEEKNRYYRE 114
           L S   GE  L + RLALS+WGCFQATNH ISSSFLEK+R+ISEQFF+LP+EEK RY RE
Sbjct: 281 LKSPSAGEGPLNDLRLALSTWGCFQATNHSISSSFLEKLRKISEQFFSLPIEEKMRYGRE 340

Query: 115 IDGHEGYGSDLILSEPQILDWTDRLYLVVNPEDERKLKFWPENPPSFREDLHEFTIKVKE 174
           +DG EGYG+DL  S  Q LDW+DRLY V +PEDER+L  WP NPPSFREDLHE+T+K+ E
Sbjct: 341 VDGMEGYGNDLTFSNQQTLDWSDRLYFVTSPEDERRLDLWPLNPPSFREDLHEYTVKIME 400

Query: 175 IIENVLIAMAASLKVEPKSFSDQVGKRPVLVTRFNFYPPCSTPHLVLGLKEHSDGSAFTI 234
           IIE VLIAMA SL VEP SF+DQVG+RP L TRFNFYPPCSTPHLVLGLKEHSDGSA TI
Sbjct: 401 IIETVLIAMARSLNVEPNSFTDQVGERPTLFTRFNFYPPCSTPHLVLGLKEHSDGSAITI 460

Query: 235 VLLDKEVEGLEMKKDNQWYRLPVPAIADSLLINVGEQGEIMSNGIFKSPVHRAVTNSERQ 294
           +LLDK+VEGL+++KD+QWYR+PVPAIADSLL+ +GEQ E+MSNGIFKS VHRAVTNSERQ
Sbjct: 461 LLLDKQVEGLQLRKDDQWYRVPVPAIADSLLLVIGEQAEVMSNGIFKSSVHRAVTNSERQ 520

Query: 295 RISVACFCCPEKDKEIKPIEGLIDERRPRLFRTVKNYVEETYFQYYQKGQRAVDELKI 353
           RISV CFCCPEKD EIKP+EGLIDE+RPRLFR+VKNY+ ETYFQ YQ+GQR VD L+I
Sbjct: 521 RISVVCFCCPEKDIEIKPVEGLIDEKRPRLFRSVKNYL-ETYFQNYQEGQRTVDGLRI 577

[Close]

BLAST of Carg27194 vs. TrEMBL
Match: tr|A0A2I4F560|A0A2I4F560_9ROSI (protein SRG1-like OS=Juglans regia OX=51240 GN=LOC108995639 PE=3 SV=1)

HSP 1 Score: 426.4 bits (1095), Expect = 6.2e-116
Identity = 207/340 (60.88%), Postives = 264/340 (77.65%), Query Frame = 0


Query: 13  QQLLINGGDTPESYIYKGGYSGGDSNNDPLPMAEIPVVDLSQLSSSQGGEAALEEFRLAL 72
           Q+LLING + PE YI+K     GD     +P+ E+PVVDLS L+SS      LE+ R AL
Sbjct: 6   QELLINGEELPEKYIHK----DGDGGVLDVPLMEVPVVDLSLLASSLTSREELEKLRSAL 65

Query: 73  SSWGCFQATNHGISSSFLEKMRQISEQFFALPMEEKNRYYREIDGHEGYGSDLILSEPQI 132
           SSWGCFQA NHGI+SSFL+K+R++++QFFALP+ EK +Y RE+D  EGYG+D+ILS  QI
Sbjct: 66  SSWGCFQAINHGITSSFLDKVREVTKQFFALPVGEKQKYSREVDEIEGYGNDMILSAQQI 125

Query: 133 LDWTDRLYLVVNPEDERKLKFWPENPPSFREDLHEFTIKVKEIIENVLIAMAASLKVEPK 192
           LDW+DRLYL VNPED+R+LKFWPE P  FR+ LH++T K+K I E VL AMA SL +E  
Sbjct: 126 LDWSDRLYLTVNPEDQRRLKFWPEIPQDFRDLLHDYTFKIKMINEVVLKAMARSLNLEDN 185

Query: 193 SFSDQVGKRPVLVTRFNFYPPCSTPHLVLGLKEHSDGSAFTIVLLDKEVEGLEMKKDNQW 252
            F DQ GKRP +  RFN+YP C  P LVLG+K H+DGSA TI+L DKEVEGL+ +KDNQW
Sbjct: 186 CFLDQYGKRPTMTARFNYYPKCPRPDLVLGVKPHADGSAITILLQDKEVEGLQFQKDNQW 245

Query: 253 YRLPVPAIADSLLINVGEQGEIMSNGIFKSPVHRAVTNSERQRISVACFCCPEKDKEIKP 312
            ++P+  I ++LLINVG+Q EIMSNGIF+SPVHR VTNSER+RIS+A FC PE +KEI+P
Sbjct: 246 NKVPI--IPEALLINVGDQVEIMSNGIFESPVHRVVTNSERERISLAVFCIPEPEKEIQP 305

Query: 313 IEGLIDERRPRLFRTVKNYVEETYFQYYQKGQRAVDELKI 353
           ++GL+++ RPRL++ VKNYV + YFQ YQ+G+R +D  KI
Sbjct: 306 VDGLVNKSRPRLYKKVKNYV-DIYFQNYQQGKRPIDAAKI 338

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BLAST of Carg27194 vs. TrEMBL
Match: tr|A0A2I4F547|A0A2I4F547_9ROSI (protein SRG1-like OS=Juglans regia OX=51240 GN=LOC108995640 PE=3 SV=1)

HSP 1 Score: 413.3 bits (1061), Expect = 5.5e-112
Identity = 201/340 (59.12%), Postives = 260/340 (76.47%), Query Frame = 0


Query: 13  QQLLINGGDTPESYIYKGGYSGGDSNNDPLPMAEIPVVDLSQLSSSQGGEAALEEFRLAL 72
           Q+L+ING + PE YI+K     GD     +P+ E+PVVDLS L+SS      LE+ R AL
Sbjct: 6   QELVINGEELPEKYIHK----DGDGGVLDVPLMEVPVVDLSLLTSSLTSREELEKLRSAL 65

Query: 73  SSWGCFQATNHGISSSFLEKMRQISEQFFALPMEEKNRYYREIDGHEGYGSDLILSEPQI 132
           SSWGCFQA NHGI+SSFL+++R +++QFFALP+ EK +Y RE+D  +GYG+ +ILS  QI
Sbjct: 66  SSWGCFQAINHGITSSFLDELRAVTKQFFALPVGEKQKYSREVDDIDGYGNGMILSAQQI 125

Query: 133 LDWTDRLYLVVNPEDERKLKFWPENPPSFREDLHEFTIKVKEIIENVLIAMAASLKVEPK 192
            DW+DRLYL VNPED+R+LKFWPE P  FR+ LH++T K+K I E VL AMA SL +E  
Sbjct: 126 PDWSDRLYLTVNPEDQRRLKFWPEIPHDFRDLLHDYTFKIKLINEVVLKAMARSLNLEDN 185

Query: 193 SFSDQVGKRPVLVTRFNFYPPCSTPHLVLGLKEHSDGSAFTIVLLDKEVEGLEMKKDNQW 252
            F DQ GKRP +  RFN+ P C  P LVLG+K H+DGSA TI+L DKEVEGL+ +KDNQW
Sbjct: 186 CFLDQYGKRPTMTARFNYCPKCPRPDLVLGVKPHADGSAITILLQDKEVEGLQFQKDNQW 245

Query: 253 YRLPVPAIADSLLINVGEQGEIMSNGIFKSPVHRAVTNSERQRISVACFCCPEKDKEIKP 312
            ++P+  I ++LLINVG+Q EIMSNGIFKSP+HR VTNSER+RIS+A FC PE +KEI+P
Sbjct: 246 NKVPI--IPEALLINVGDQVEIMSNGIFKSPLHRVVTNSERERISLAVFCIPESEKEIEP 305

Query: 313 IEGLIDERRPRLFRTVKNYVEETYFQYYQKGQRAVDELKI 353
           ++GL+++ RPRL++ VKNYV + YFQ YQ+G+R +D  KI
Sbjct: 306 VDGLVNKSRPRLYKKVKNYV-DIYFQNYQQGKRPIDAAKI 338

[Close]

BLAST of Carg27194 vs. TrEMBL
Match: tr|A0A2P4LKB4|A0A2P4LKB4_QUESU (Protein srg1 OS=Quercus suber OX=58331 GN=CFP56_39790 PE=3 SV=1)

HSP 1 Score: 407.1 bits (1045), Expect = 3.9e-110
Identity = 198/340 (58.24%), Postives = 257/340 (75.59%), Query Frame = 0


Query: 13  QQLLINGGDTPESYIYKGGYSGGDSNNDPLPMAEIPVVDLSQL-SSSQGGEAALEEFRLA 72
           Q+L+INGG  P++Y+YK    GG   + PL   EIPV+DL  L SS+   E  LE+ R A
Sbjct: 29  QELVINGGQPPDNYVYKNSTGGGPDVHTPL--NEIPVIDLGNLKSSATTEEEELEKLRSA 88

Query: 73  LSSWGCFQATNHGISSSFLEKMRQISEQFFALPMEEKNRYYREIDGHEGYGSDLILSEPQ 132
            SSWGCFQA NHG++SSFL+K+ ++++QFF+LPM+EK  Y RE +  EGYG+D+I S+ Q
Sbjct: 89  FSSWGCFQAINHGMTSSFLDKVHEVTKQFFSLPMDEKREYSREANNIEGYGNDIIFSDHQ 148

Query: 133 ILDWTDRLYLVVNPEDERKLKFWPENPPSFREDLHEFTIKVKEIIENVLIAMAASLKVEP 192
           ILDWTDRL L+V+PED+RK  FWP NP  FR+ L ++T+++  I E +L AMA SL +E 
Sbjct: 149 ILDWTDRLILMVSPEDQRKFTFWPNNPTDFRDILLKYTMELSLITEVILKAMAKSLNLED 208

Query: 193 KSFSDQVGKRPVLVTRFNFYPPCSTPHLVLGLKEHSDGSAFTIVLLDKEVEGLEMKKDNQ 252
             F +Q+G+R  ++TRFN YPPC  P LVLGLK H+DGSA T VL DKEVEGL+  KDNQ
Sbjct: 209 NCFLEQMGERSKMLTRFNLYPPCPRPDLVLGLKPHADGSAITFVLQDKEVEGLQFLKDNQ 268

Query: 253 WYRLPVPAIADSLLINVGEQGEIMSNGIFKSPVHRAVTNSERQRISVACFCCPEKDKEIK 312
           W R+PV  I  +LLIN+G+Q EIMSNGIFKSPVHR VTNSER+RISVA FC P+ D EI+
Sbjct: 269 WIRVPV--IPQALLINIGDQAEIMSNGIFKSPVHRVVTNSERERISVAVFCSPDSDIEIE 328

Query: 313 PIEGLIDERRPRLFRTVKNYVEETYFQYYQKGQRAVDELK 352
           PI  L+++ RPRL++ VKNY  +TYFQYYQ+G+RA+D ++
Sbjct: 329 PIGRLVNDSRPRLYKKVKNYT-DTYFQYYQQGKRAIDAVR 363

The following BLAST results are available for this feature:

BLAST of Carg27194 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_008465534.1	6.4e-157	77.46	PREDICTED: protein SRG1-like [Cucumis melo]	[more]
XP_011655280.1	4.2e-156	77.97	PREDICTED: protein SRG1-like [Cucumis sativus]	[more]
XP_011655698.1	1.6e-155	77.68	PREDICTED: protein SRG1-like [Cucumis sativus]	[more]
XP_022134811.1	6.0e-155	77.62	uncharacterized protein LOC111006992 [Momordica charantia]	[more]
KGN65097.1	1.9e-132	76.17	hypothetical protein Csa_1G212830 [Cucumis sativus]	[more]

BLAST of Carg27194 vs. TAIR10


Match Name	E-value	Identity	Description
AT5G20400.1	2.0e-99	50.72	2-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily protein	[more]
AT5G54000.1	2.7e-96	48.14	2-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily protein	[more]
AT5G20550.1	1.5e-94	49.27	2-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily protein	[more]
AT1G49390.1	5.5e-94	47.97	2-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily protein	[more]
AT3G21420.1	7.5e-59	38.66	2-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily protein	[more]

BLAST of Carg27194 vs. Swiss-Prot


Match Name	E-value	Identity	Description
sp\|Q39224\|SRG1_ARATH	9.8e-56	35.90	Protein SRG1 OS=Arabidopsis thaliana OX=3702 GN=SRG1 PE=2 SV=1	[more]
sp\|A2A1A0\|NCS1_COPJA	1.7e-52	38.69	S-norcoclaurine synthase 1 OS=Coptis japonica OX=3442 GN=NCS1 PE=1 SV=1	[more]
sp\|O80449\|DIOX4_ARATH	5.0e-52	35.66	Probable 2-oxoglutarate-dependent dioxygenase ANS OS=Arabidopsis thaliana OX=370...	[more]
sp\|D4N502\|DIOX3_PAPSO	1.2e-50	38.52	Codeine O-demethylase OS=Papaver somniferum OX=3469 GN=CODM PE=1 SV=1	[more]
sp\|D4N501\|DIOX2_PAPSO	3.4e-48	34.62	Probable 2-oxoglutarate/Fe(II)-dependent dioxygenase OS=Papaver somniferum OX=34...	[more]

BLAST of Carg27194 vs. TrEMBL


Match Name	E-value	Identity	Description
tr\|A0A1S3CP07\|A0A1S3CP07_CUCME	4.3e-157	77.46	protein SRG1-like OS=Cucumis melo OX=3656 GN=LOC103503159 PE=3 SV=1	[more]
tr\|A0A0A0LW96\|A0A0A0LW96_CUCSA	1.2e-132	76.17	Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_1G212830 PE=3 SV=1	[more]
tr\|A0A2I4F560\|A0A2I4F560_9ROSI	6.2e-116	60.88	protein SRG1-like OS=Juglans regia OX=51240 GN=LOC108995639 PE=3 SV=1	[more]
tr\|A0A2I4F547\|A0A2I4F547_9ROSI	5.5e-112	59.12	protein SRG1-like OS=Juglans regia OX=51240 GN=LOC108995640 PE=3 SV=1	[more]
tr\|A0A2P4LKB4\|A0A2P4LKB4_QUESU	3.9e-110	58.24	Protein srg1 OS=Quercus suber OX=58331 GN=CFP56_39790 PE=3 SV=1	[more]

Annotated Terms

The following terms have been associated with this gene:

Vocabulary: **Biological Process**
Term	Definition
GO:0055114	oxidation-reduction process

Vocabulary: **Molecular Function**
Term	Definition
GO:0016491	oxidoreductase activity

Vocabulary: **INTERPRO**
Term	Definition
IPR026992	DIOX_N
IPR027443	IPNS-like
IPR005123	Oxoglu/Fe-dep_dioxygenase

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0055114	oxidation-reduction process
cellular_component	GO:0005575	cellular_component
molecular_function	GO:0016491	oxidoreductase activity

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Carg27194-RA	Carg27194-RA	mRNA

InterPro

Analysis Name: InterPro Annotations of silver-seed gourd
Date Performed: 2019-03-07


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR005123	Oxoglutarate/iron-dependent dioxygenase	PFAM	PF03171	2OG-FeII_Oxy	coord: 205..304 e-value: 4.7E-20 score: 71.8
IPR005123	Oxoglutarate/iron-dependent dioxygenase	PROSITE	PS51471	FE2OG_OXY	coord: 202..304 score: 11.181
IPR027443	Isopenicillin N synthase-like	GENE3D	G3DSA:2.60.120.330		coord: 20..343 e-value: 2.0E-90 score: 305.5
IPR026992	Non-haem dioxygenase N-terminal domain	PFAM	PF14226	DIOX_N	coord: 47..156 e-value: 2.6E-25 score: 89.4
None	No IPR available	PANTHER	PTHR10209	OXIDOREDUCTASE, 2OG-FE II OXYGENASE FAMILY PROTEIN	coord: 12..349
None	No IPR available	PANTHER	PTHR10209:SF373	SUBFAMILY NOT NAMED	coord: 12..349
None	No IPR available	SUPERFAMILY	SSF51197	Clavaminate synthase-like	coord: 11..341

Synteny

The following gene(s) are orthologous to this gene:


Gene	Orthologue	Organism	Block
Carg27194	Cla012183	Watermelon (97103) v1	carwmB0572
Carg27194	Csa1G263950	Cucumber (Chinese Long) v2	carcuB0602
Carg27194	ClCG06G011790	Watermelon (Charleston Gray)	carwcgB0560
Carg27194	CSPI01G17040	Wild cucumber (PI 183967)	carcpiB0608
Carg27194	Cucsa.350520	Cucumber (Gy14) v1	carcgyB0649
Carg27194	CmaCh11G005090	Cucurbita maxima (Rimu)	carcmaB0804
Carg27194	CmaCh10G005090	Cucurbita maxima (Rimu)	carcmaB0803
Carg27194	CmoCh11G005060	Cucurbita moschata (Rifu)	carcmoB0781
Carg27194	CmoCh10G005460	Cucurbita moschata (Rifu)	carcmoB0780
Carg27194	Cp4.1LG04g09110	Cucurbita pepo (Zucchini)	carcpeB0819
Carg27194	Cp4.1LG18g05360	Cucurbita pepo (Zucchini)	carcpeB0818
Carg27194	CsGy1G017260	Cucumber (Gy14) v2	carcgybB0576
Carg27194	CsaV3_1G028060	Cucumber (Chinese Long) v3	carcucB0609
Carg27194	Cla97C06G120950	Watermelon (97103) v2	carwmbB0600
Carg27194	Bhi02G000754	Wax gourd	carwgoB0686

The following gene(s) are paralogous to this gene:


Gene	Paralogue	Organism	Block
Carg27194	Carg09953	Silver-seed gourd	carcarB153

The following block(s) are covering this gene:


Gene	Organism	Block
Carg27194	Melon (DHL92) v3.5.1	carmeB0578
Carg27194	Melon (DHL92) v3.6.1	carmedB0585