BLAST of Cucsa.328080 vs. Swiss-Prot
Match:
RGA2_SOLBU (Disease resistance protein RGA2 OS=Solanum bulbocastanum GN=RGA2 PE=1 SV=1)
HSP 1 Score: 516.9 bits (1330), Expect = 5.1e-145
Identity = 346/1012 (34.19%), Postives = 534/1012 (52.77%), Query Frame = 1
Query: 13 IITKLGSFALQDLGLLWGFHDELDKLKGTVSALEAVLLDAEEKQSKSRAVKDWILKLKDT 72
++ L SF +L LL+GF DE +L S ++AVL DA+EKQ ++ +++W+ KL
Sbjct: 9 LLDNLTSFLKGELVLLFGFQDEFQRLSSMFSTIQAVLEDAQEKQLNNKPLENWLQKLNAA 68
Query: 73 FYDIDDLLDVFSYESLKRQVMTKHRTNNTKKVRIFFSKSNQ--IAFRLKMSQKIKRVREK 132
Y++DD+LD +++T T+ + + + + I FR K+ +++ +V +K
Sbjct: 69 TYEVDDILD-------------EYKTKATRFSQSEYGRYHPKVIPFRHKVGKRMDQVMKK 128
Query: 133 LDAIAMDKTQFNLYENTREIQDDESTKRLETTSFIREGEIIGRDDDKKSIIHYLLDTNIH 192
L AIA ++ F+L+E E Q R ET S + E ++ GRD +K I+ L++
Sbjct: 129 LKAIAEERKNFHLHEKIVERQ----AVRRETGSVLTEPQVYGRDKEKDEIVKILINNVSD 188
Query: 193 EDSVAVIAIIGMGGLGKTALVQSIYGDEKVKKHFELTMWVCISEEFDVKVIIEKIIESLT 252
++V+ I+GMGGLGKT L Q ++ D++V +HF +W+C+SE+FD K +I+ I+ES+
Sbjct: 189 AQHLSVLPILGMGGLGKTTLAQMVFNDQRVTEHFHSKIWICVSEDFDEKRLIKAIVESIE 248
Query: 253 KKKREPDLQLDTLQSMVREKIDGKRYLLVMDDVWNVNRAKWISLKRYLMGGAKGSRILIT 312
+ ++ L LQ ++E ++GKRYLLV+DDVWN ++ KW +L+ L GA G+ +L T
Sbjct: 249 GRPLLGEMDLAPLQKKLQELLNGKRYLLVLDDVWNEDQQKWANLRAVLKVGASGASVLTT 308
Query: 313 TRTHQVAQTFETILSHHLKELDEEKSWKLFRKMAFSNESEVLENSKLVVIGKEIVTKLKG 372
TR +V T+ + L L +E W LF + AF ++ E+ N LV IGKEIV K G
Sbjct: 309 TRLEKVGSIMGTLQPYELSNLSQEDCWLLFMQRAFGHQEEI--NPNLVAIGKEIVKKSGG 368
Query: 373 SPLAIRVIGSYLYSKKSEKDWLSFKDHELDTIMQQENEIQSILKISFNHLSSSLKHCFTY 432
PLA + +G L K+ E+ W +D + + Q E+ I L++S++ L LK CF Y
Sbjct: 369 VPLAAKTLGGILCFKREERAWEHVRDSPIWNLPQDESSILPALRLSYHQLPLDLKQCFAY 428
Query: 433 CALFSKDYHYEIRKNDLIKQWMAQGFIQPHNKKAMEDVGDDYFEELLGRSFFQDIRKNKW 492
CA+F KD E K LI WMA GF+ +EDVGD+ ++EL RSFFQ+I
Sbjct: 429 CAVFPKDAKME--KEKLISLWMAHGFLLSKGNMELEDVGDEVWKELYLRSFFQEI----- 488
Query: 493 GEIKK----FKMHDIIHDLACSVVENDCVLANDDTKSIDKRTRHVSISAFNSMTRWKLIT 552
E+K FKMHD+IHDLA S+ AN + +I + +H S T +++
Sbjct: 489 -EVKDGKTYFKMHDLIHDLATSLFS-----ANTSSSNIREINKH-------SYTH--MMS 548
Query: 553 KSLIEAKNLRTLNYARRHHIDLSNHLRLRTLNL---EFHFVPKCIGKMKHLRYINITYCY 612
E TL L + LR LNL F+ +P IG + HLRY+N+
Sbjct: 549 IGFAEVVFFYTLP-------PLEKFISLRVLNLGDSTFNKLPSSIGDLVHLRYLNLYGSG 608
Query: 613 IDFLPKAVTKLYHLETLIIRGCLELRELSSDIKNLINLRHLDIKDFKHVWSYMPKGMGSM 672
+ LPK + KL +L+TL ++ C +L L + L +LR+L + D + MP +GS+
Sbjct: 609 MRSLPKQLCKLQNLQTLDLQYCTKLCCLPKETSKLGSLRNL-LLDGSQSLTCMPPRIGSL 668
Query: 673 TTLQTMNLFILGENKGGELSELNGLVNLRGSLSIQQLQFCKPIGLENVKYLEEKSRIQKL 732
T L+T+ F++G KG +L EL G +NL GS+ I L+ K L K + L
Sbjct: 669 TCLKTLGQFVVGRKKGYQLGEL-GNLNLYGSIKISHLERVKNDKDAKEANLSAKGNLHSL 728
Query: 733 ELHWKTYQRESKIDDEDERVLESLKPHSNLQKIRIEGYRGLKLCNWFSFDSIVNLVFIKL 792
+ W + + E+ +VLE+LKPHSNL ++I G+RG+ L W + + N+V I +
Sbjct: 729 SMSWNNFGPHI-YESEEVKVLEALKPHSNLTSLKIYGFRGIHLPEWMNHSVLKNIVSILI 788
Query: 793 FNCEKLQQLPRFDRFPFLKHLHLE----DLPSIEYIAINNYVSSSMTTFFPSLENLSIIK 852
N LP F P L+ L L D+ +E + I+ + FPSL L I
Sbjct: 789 SNFRNCSCLPPFGDLPCLESLELHWGSADVEYVEEVDIDVHSGFPTRIRFPSLRKLDIWD 848
Query: 853 LPNLKEWWKGESIDQNTSFPTILRHLSQLKIHYCRQLASIPQHGPLQSLDIRDISLQLFE 912
+LK K E +Q FP L ++ IH C P +L +L
Sbjct: 849 FGSLKGLLKKEGEEQ---FPV----LEEMIIHEC----------PFLTLSSNLRALTSLR 908
Query: 913 LVIKMTATNIIFLPNDLFSNVTHLQSLVIGRCFNLKMSFDDDNVRWKELGSLRTLRLCFI 972
+ AT+ P ++F N+ +L+ L I RC NLK + L +L++L++
Sbjct: 909 ICYNKVATS---FPEEMFKNLANLKYLTISRCNNLK----ELPTSLASLNALKSLKIQLC 945
Query: 973 PKLEYLP-KGFQYLKALEHLELLWCENLACI-LGIEHLTSLSRLEISNCPNL 1010
LE LP +G + L +L L + C L C+ G++HLT+L+ L+I CP L
Sbjct: 969 CALESLPEEGLEGLSSLTELFVEHCNMLKCLPEGLQHLTTLTSLKIRGCPQL 945
BLAST of Cucsa.328080 vs. Swiss-Prot
Match:
RGA3_SOLBU (Putative disease resistance protein RGA3 OS=Solanum bulbocastanum GN=RGA3 PE=2 SV=2)
HSP 1 Score: 505.0 bits (1299), Expect = 2.0e-141
Identity = 353/1046 (33.75%), Postives = 551/1046 (52.68%), Query Frame = 1
Query: 1 MAESILCSLAGSIITKLGSFALQDLGLLWGFHDELDKLKGTVSALEAVLLDAEEKQSKSR 60
MAE+ L L + L F +LGL++GF E KL S ++AVL DA+EKQ K +
Sbjct: 1 MAEAFLQVL----LDNLTFFIQGELGLVFGFEKEFKKLSSMFSMIQAVLEDAQEKQLKYK 60
Query: 61 AVKDWILKLKDTFYDIDDLLDVFSYES--LKRQVMTKHRTNNTKKVRIFFSKSNQIAFRL 120
A+K+W+ KL Y++DD+LD E+ K+ V+ ++ I F
Sbjct: 61 AIKNWLQKLNVAAYEVDDILDDCKTEAARFKQAVLGRYHPRT-------------ITFCY 120
Query: 121 KMSQKIKRVREKLDAIAMDKTQFNLYENTREIQDDESTKRLETTSFIREGEIIGRDDDKK 180
K+ +++K + EKLDAIA ++ F+L E E Q R +T + E ++ GR+ ++
Sbjct: 121 KVGKRMKEMMEKLDAIAEERRNFHLDERIIERQ----AARRQTGFVLTEPKVYGREKEED 180
Query: 181 SIIHYLLDTNIHEDSVAVIAIIGMGGLGKTALVQSIYGDEKVKKHFELTMWVCISEEFDV 240
I+ L++ + + V V+ I+GMGGLGKT L Q ++ D+++ +HF L +WVC+S++FD
Sbjct: 181 EIVKILINNVSYSEEVPVLPILGMGGLGKTTLAQMVFNDQRITEHFNLKIWVCVSDDFDE 240
Query: 241 KVIIEKIIESLTKKKREPDLQLDTLQSMVREKIDGKRYLLVMDDVWNVNRAKWISLKRYL 300
K +I+ I+ES+ + K D+ L LQ ++E ++GKRY LV+DDVWN ++ KW +L+ L
Sbjct: 241 KRLIKAIVESI-EGKSLGDMDLAPLQKKLQELLNGKRYFLVLDDVWNEDQEKWDNLRAVL 300
Query: 301 MGGAKGSRILITTRTHQVAQTFETILSHHLKELDEEKSWKLFRKMAFSNESEVLENSKLV 360
GA G+ ILITTR ++ T+ + L L +E W LF++ AF +++E + KL+
Sbjct: 301 KIGASGASILITTRLEKIGSIMGTLQLYQLSNLSQEDCWLLFKQRAFCHQTET--SPKLM 360
Query: 361 VIGKEIVTKLKGSPLAIRVIGSYLYSKKSEKDWLSFKDHELDTIMQQENEIQSILKISFN 420
IGKEIV K G PLA + +G L K+ E +W +D E+ + Q EN + L++S++
Sbjct: 361 EIGKEIVKKCGGVPLAAKTLGGLLRFKREESEWEHVRDSEIWNLPQDENSVLPALRLSYH 420
Query: 421 HLSSSLKHCFTYCALFSKDYHYEIRKNDLIKQWMAQGFIQPHNKKAMEDVGDDYFEELLG 480
HL L+ CF YCA+F KD +I K LI WMA F+ +EDVG++ + EL
Sbjct: 421 HLPLDLRQCFAYCAVFPKD--TKIEKEYLIALWMAHSFLLSKGNMELEDVGNEVWNELYL 480
Query: 481 RSFFQDIRKNKWGEIKK----FKMHDIIHDLACSVVENDCVLANDDTKSIDKRTRHVSIS 540
RSFFQ+I E+K FKMHD+IHDLA S+ A+ ++SI R +++
Sbjct: 481 RSFFQEI------EVKSGKTYFKMHDLIHDLATSMFS-----ASASSRSI----RQINVK 540
Query: 541 AFNSMTRWKLITKSLIEAKNLRTLNYARRHHIDL-SNHLRLRTLNL---EFHFVPKCIGK 600
M I + + ++ + L + LR LNL EF +P +G
Sbjct: 541 DDEDM---MFIVTNYKDMMSIGFSEVVSSYSPSLFKRFVSLRVLNLSNSEFEQLPSSVGD 600
Query: 601 MKHLRYINITYCYIDFLPKAVTKLYHLETLIIRGCLELRELSSDIKNLINLRHLDIKDFK 660
+ HLRY++++ I LPK + KL +L+TL + C L L L +LR+L + D
Sbjct: 601 LVHLRYLDLSGNKICSLPKRLCKLQNLQTLDLYNCQSLSCLPKQTSKLCSLRNL-VLDHC 660
Query: 661 HVWSYMPKGMGSMTTLQTMNLFILGENKGGELSELNGLVNLRGSLSIQQLQFCKPIGLEN 720
+ S MP +G +T L+T+ F++GE KG +L EL L NLRG++SI L+ K
Sbjct: 661 PLTS-MPPRIGLLTCLKTLGYFVVGERKGYQLGELRNL-NLRGAISITHLERVKNDMEAK 720
Query: 721 VKYLEEKSRIQKLELHWKTYQRESKIDDEDERVLESLKPHSNLQKIRIEGYRGLKLCNWF 780
L K+ + L + W R ++ + E+ +VLE+LKPH NL+ + I + G L +W
Sbjct: 721 EANLSAKANLHSLSMSW---DRPNRYESEEVKVLEALKPHPNLKYLEIIDFCGFCLPDWM 780
Query: 781 SFDSIVNLVFIKLFNCEKLQQLPRFDRFPFLKHLHLED-LPSIEYIAINNYVSSSMTTFF 840
+ + N+V I + CE LP F P L+ L L+D +EY+ + +++ F
Sbjct: 781 NHSVLKNVVSILISGCENCSCLPPFGELPCLESLELQDGSVEVEYVEDSGFLTRRR---F 840
Query: 841 PSLENLSIIKLPNLKEWWKGESIDQNTSFPTILRHLSQLKIHYCRQLASIPQHGPLQSLD 900
PSL L I NLK + + +Q FP L ++KI C P S
Sbjct: 841 PSLRKLHIGGFCNLKGLQRMKGAEQ---FPV----LEEMKISDCPMFVF-----PTLS-- 900
Query: 901 IRDISLQLFELVIKMTATNIIFLPNDLFSNVTHLQSLVIGRCFNLKMSFDDDNVRWKELG 960
S++ E+ + A + SN++ L SL I + ++ +K L
Sbjct: 901 ----SVKKLEIWGEADAGGL-----SSISNLSTLTSLKIFSNHTVTSLLEE---MFKNLE 960
Query: 961 SLRTLRLCFIPKLEYLPKGFQYLKALEHLELLWCENLACI--LGIEHLTSLSRLEISNCP 1020
+L L + F+ L+ LP L L+ L++ +C L + G+E L+SL+ L + +C
Sbjct: 961 NLIYLSVSFLENLKELPTSLASLNNLKCLDIRYCYALESLPEEGLEGLSSLTELFVEHCN 967
Query: 1021 NLTSLPEGMTQLISLTCLIIDDCPNL 1034
L LPEG+ L +LT L I CP L
Sbjct: 1021 MLKCLPEGLQHLTTLTSLKIRGCPQL 967
HSP 2 Score: 47.8 bits (112), Expect = 8.8e-04
Identity = 33/81 (40.74%), Postives = 46/81 (56.79%), Query Frame = 1
Query: 965 LPKGFQYLKALEHLELLWCENLACI-LGIEHLTSLSRLEISNCPNLTSLPE-GMTQLISL 1024
L + F+ L+ L +L + + ENL + + L +L L+I C L SLPE G+ L SL
Sbjct: 873 LEEMFKNLENLIYLSVSFLENLKELPTSLASLNNLKCLDIRYCYALESLPEEGLEGLSSL 932
Query: 1025 TCLIIDDCPNLSTLPEGLHHL 1044
T L ++ C L LPEGL HL
Sbjct: 933 TELFVEHCNMLKCLPEGLQHL 953
BLAST of Cucsa.328080 vs. Swiss-Prot
Match:
RGA1_SOLBU (Putative disease resistance protein RGA1 OS=Solanum bulbocastanum GN=RGA1 PE=2 SV=2)
HSP 1 Score: 491.5 bits (1264), Expect = 2.3e-137
Identity = 343/1028 (33.37%), Postives = 542/1028 (52.72%), Query Frame = 1
Query: 13 IITKLGSFALQDLGLLWGFHDELDKLKGTVSALEAVLLDAEEKQSKSRAVKDWILKLKDT 72
++ L SF +L LL+GF DE +L S ++AVL DA+EKQ + +++W+ KL
Sbjct: 9 VLDNLTSFLKGELVLLFGFQDEFQRLSSMFSTIQAVLEDAQEKQLNDKPLENWLQKLNAA 68
Query: 73 FYDIDDLLDVFSYESLKRQVMTKHRTNNTKKVRIFFSKSNQ--IAFRLKMSQKIKRVREK 132
Y++DD+LD +++T T+ ++ + + + I FR K+ +++ +V +K
Sbjct: 69 TYEVDDILD-------------EYKTKATRFLQSEYGRYHPKVIPFRHKVGKRMDQVMKK 128
Query: 133 LDAIAMDKTQFNLYENTREIQDDESTKRLETTSFIREGEIIGRDDDKKSIIHYLLDTNIH 192
L+AIA ++ +F+L E E Q +T+ ET S + E ++ GRD +K I+ L++T
Sbjct: 129 LNAIAEERKKFHLQEKIIERQ--AATR--ETGSVLTEPQVYGRDKEKDEIVKILINTASD 188
Query: 193 EDSVAVIAIIGMGGLGKTALVQSIYGDEKVKKHFELTMWVCISEEFDVKVIIEKIIESLT 252
++V+ I+GMGGLGKT L Q ++ D++V + F +W+CIS++F+ K +I+ I+ES+
Sbjct: 189 AQKLSVLPILGMGGLGKTTLSQMVFNDQRVTERFYPKIWICISDDFNEKRLIKAIVESIE 248
Query: 253 KKKREPDLQLDTLQSMVREKIDGKRYLLVMDDVWNVNRAKWISLKRYLMGGAKGSRILIT 312
K D+ L LQ ++E ++GKRY LV+DDVWN ++ KW +L+ L GA G+ +L T
Sbjct: 249 GKSLS-DMDLAPLQKKLQELLNGKRYFLVLDDVWNEDQHKWANLRAVLKVGASGAFVLTT 308
Query: 313 TRTHQVAQTFETILSHHLKELDEEKSWKLFRKMAFSNESEVLENSKLVVIGKEIVTKLKG 372
TR +V T+ + L L E W LF + AF ++ E+ N L+ IGKEIV K G
Sbjct: 309 TRLEKVGSIMGTLQPYELSNLSPEDCWFLFMQRAFGHQEEI--NPNLMAIGKEIVKKCGG 368
Query: 373 SPLAIRVIGSYLYSKKSEKDWLSFKDHELDTIMQQENEIQSILKISFNHLSSSLKHCFTY 432
PLA + +G L K+ E++W +D + + Q E+ I L++S++HL L+ CF Y
Sbjct: 369 VPLAAKTLGGILRFKREEREWEHVRDSPIWNLPQDESSILPALRLSYHHLPLDLRQCFVY 428
Query: 433 CALFSKDYHYEIRKNDLIKQWMAQGFIQPHNKKAMEDVGDDYFEELLGRSFFQDIRKNKW 492
CA+F KD ++ K +LI WMA GF+ +EDVG++ + EL RSFFQ+I
Sbjct: 429 CAVFPKD--TKMAKENLIAFWMAHGFLLSKGNLELEDVGNEVWNELYLRSFFQEIEVESG 488
Query: 493 GEIKKFKMHDIIHDLACSVVENDCVLANDDTKSIDKRTRHVSISAFNSMTRWKLITKSLI 552
FKMHD+IHDLA S L + +T S + R + + + + + S
Sbjct: 489 KTY--FKMHDLIHDLATS-------LFSANTSSSNIREINANYDGYMMSIGFAEVVSSYS 548
Query: 553 EAKNLRTLNYARRHHIDLSNHLRLRTLNL---EFHFVPKCIGKMKHLRYINITYCY-IDF 612
+ L + LR LNL + +P IG + HLRY++++ + I
Sbjct: 549 PSL--------------LQKFVSLRVLNLRNSNLNQLPSSIGDLVHLRYLDLSGNFRIRN 608
Query: 613 LPKAVTKLYHLETLIIRGCLELRELSSDIKNLINLRHLDIKDFKHVWSYMPKGMGSMTTL 672
LPK + KL +L+TL + C L L L +LR+L + D + S P+ +G +T L
Sbjct: 609 LPKRLCKLQNLQTLDLHYCDSLSCLPKQTSKLGSLRNL-LLDGCSLTSTPPR-IGLLTCL 668
Query: 673 QTMNLFILGENKGGELSELNGLVNLRGSLSIQQLQFCKPIGLENVKYLEEKSRIQKLELH 732
++++ F++G+ KG +L EL L NL GS+SI +L K L K+ + L L
Sbjct: 669 KSLSCFVIGKRKGHQLGELKNL-NLYGSISITKLDRVKKDTDAKEANLSAKANLHSLCLS 728
Query: 733 WKTYQRESKIDDEDERVLESLKPHSNLQKIRIEGYRGLKLCNWFSFDSIVNLVFIKLFNC 792
W + D VLE+LKPHSNL+ + I G+ G++L +W + + N+V I++ C
Sbjct: 729 WDLDGKHR----YDSEVLEALKPHSNLKYLEINGFGGIRLPDWMNQSVLKNVVSIRIRGC 788
Query: 793 EKLQQLPRFDRFPFLKHLHLE-DLPSIEYIAINNYVSSSMTTFFPSLENLSIIKLPNLKE 852
E LP F P L+ L L +EY+ N + FPSL L I NLK
Sbjct: 789 ENCSCLPPFGELPCLESLELHTGSADVEYVEDNVHPGR-----FPSLRKLVIWDFSNLKG 848
Query: 853 WWKGESIDQNTSFPTILRHLSQLKIHYCRQLASIPQHGPLQSLDIRDISLQLFELVIKMT 912
K E Q FP L ++ ++C IP +++L K+
Sbjct: 849 LLKMEGEKQ---FPV----LEEMTFYWCPMFV-IPTLSSVKTL--------------KVI 908
Query: 913 ATNIIFLPNDLFSNVTHLQSLVIGRCFNLKMSFDDDNVRWKELGSLRTLRLCFIPKLEYL 972
T+ L + SN+ L SL I N++ + + + +K L +L+ L++ F L+ L
Sbjct: 909 VTDATVLRS--ISNLRALTSLDISD--NVEATSLPEEM-FKSLANLKYLKISFFRNLKEL 952
Query: 973 PKGFQYLKALEHLELLWCENLACI--LGIEHLTSLSRLEISNCPNLTSLPEGMTQLISLT 1032
P L AL+ L+ +C+ L + G++ LTSL+ L +SNC L LPEG+ L +LT
Sbjct: 969 PTSLASLNALKSLKFEFCDALESLPEEGVKGLTSLTELSVSNCMMLKCLPEGLQHLTALT 952
HSP 2 Score: 35.0 bits (79), Expect = 5.9e+00
Identity = 37/129 (28.68%), Postives = 52/129 (40.31%), Query Frame = 1
Query: 936 MSFDDDNVRWKELGSLRTLRLCFIPKLEYLPK--GFQYLKALEHLELLWCE--------- 995
+ + +DNV SLR L + L+ L K G + LE + WC
Sbjct: 763 VEYVEDNVHPGRFPSLRKLVIWDFSNLKGLLKMEGEKQFPVLEEMTFYWCPMFVIPTLSS 822
Query: 996 ---------NLACILGIEHLTSLSRLEISNCPNLTSLPEGM-TQLISLTCLIIDDCPNLS 1044
+ + I +L +L+ L+IS+ TSLPE M L +L L I NL
Sbjct: 823 VKTLKVIVTDATVLRSISNLRALTSLDISDNVEATSLPEEMFKSLANLKYLKISFFRNLK 882
HSP 3 Score: 31.6 bits (70), Expect = 6.5e+01
Identity = 25/74 (33.78%), Postives = 30/74 (40.54%), Query Frame = 1
Query: 995 LTSLSRLEISNCPNLTSLP-------------------------EGMTQLISLTCLIIDD 1044
L +L L+IS NL LP EG+ L SLT L + +
Sbjct: 867 LANLKYLKISFFRNLKELPTSLASLNALKSLKFEFCDALESLPEEGVKGLTSLTELSVSN 926
BLAST of Cucsa.328080 vs. Swiss-Prot
Match:
RGA4_SOLBU (Putative disease resistance protein RGA4 OS=Solanum bulbocastanum GN=RGA4 PE=2 SV=1)
HSP 1 Score: 487.6 bits (1254), Expect = 3.3e-136
Identity = 342/1040 (32.88%), Postives = 537/1040 (51.63%), Query Frame = 1
Query: 1 MAESILCSLAGSIITKLGSFALQDLGLLWGFHDELDKLKGTVSALEAVLLDAEEKQSKSR 60
MAE+ L L + L SF L L++GF E +KL S ++AVL DA+EKQ K +
Sbjct: 1 MAEAFLQVL----LENLTSFIGDKLVLIFGFEKECEKLSSVFSTIQAVLQDAQEKQLKDK 60
Query: 61 AVKDWILKLKDTFYDIDDLLDVFSYESLKRQVMTKHRTNNTKKVRIFFSKSNQIAFRLKM 120
A+++W+ KL Y++DD+L E+++ + + R+ F I FR K+
Sbjct: 61 AIENWLQKLNSAAYEVDDILGECKNEAIRFE-----------QSRLGFYHPGIINFRHKI 120
Query: 121 SQKIKRVREKLDAIAMDKTQFNLYENTREIQDDESTKRLETTSFIREGEIIGRDDDKKSI 180
+++K + EKLDAI+ ++ +F+ E E Q +T+ ET + E ++ GRD ++ I
Sbjct: 121 GRRMKEIMEKLDAISEERRKFHFLEKITERQAAAATR--ETGFVLTEPKVYGRDKEEDEI 180
Query: 181 IHYLLDTNIHEDSVAVIAIIGMGGLGKTALVQSIYGDEKVKKHFELTMWVCISEEFDVKV 240
+ L++ + + V IIGMGGLGKT L Q I+ DE+V KHF +WVC+S++FD K
Sbjct: 181 VKILINNVNVAEELPVFPIIGMGGLGKTTLAQMIFNDERVTKHFNPKIWVCVSDDFDEKR 240
Query: 241 IIEKIIESLTKKKREPDLQLDTLQSMVREKIDGKRYLLVMDDVWNVNRAKWISLKRYLMG 300
+I+ II ++ + + L + Q ++E ++GKRYLLV+DDVWN + KW L+ L
Sbjct: 241 LIKTIIGNIERSSPHVE-DLASFQKKLQELLNGKRYLLVLDDVWNDDLEKWAKLRAVLTV 300
Query: 301 GAKGSRILITTRTHQVAQTFETILSHHLKELDEEKSWKLFRKMAFSNESEVLENSKLVVI 360
GA+G+ IL TTR +V T+ +HL L S LF + AF + E N LV I
Sbjct: 301 GARGASILATTRLEKVGSIMGTLQPYHLSNLSPHDSLLLFMQRAFGQQKEA--NPNLVAI 360
Query: 361 GKEIVTKLKGSPLAIRVIGSYLYSKKSEKDWLSFKDHELDTIMQQENEIQSILKISFNHL 420
GKEIV K G PLA + +G L K+ E +W +D+E+ ++ Q E+ I L++S++HL
Sbjct: 361 GKEIVKKCGGVPLAAKTLGGLLRFKREESEWEHVRDNEIWSLPQDESSILPALRLSYHHL 420
Query: 421 SSSLKHCFTYCALFSKDYHYEIRKNDLIKQWMAQGFIQPHNKKAMEDVGDDYFEELLGRS 480
L+ CF YCA+F KD ++ K +LI WMA GF+ +EDVG++ + EL RS
Sbjct: 421 PLDLRQCFAYCAVFPKD--TKMIKENLITLWMAHGFLLSKGNLELEDVGNEVWNELYLRS 480
Query: 481 FFQDIRKNKWGEIKKFKMHDIIHDLACSVVENDCVLANDDTKSIDKRTRHVSISAFNSMT 540
FFQ+I FK+HD+IHDLA S+ N ++ VSI ++
Sbjct: 481 FFQEIEAKSGNTY--FKIHDLIHDLATSLFSASASCGNIREINVKDYKHTVSIGFAAVVS 540
Query: 541 RWKLITKSLIEAKNLRTLNYARRHHIDLSNHLRLRTLNLEF---HFVPKCIGKMKHLRYI 600
+ + SL L + LR LNL + +P IG + HLRY+
Sbjct: 541 SY---SPSL------------------LKKFVSLRVLNLSYSKLEQLPSSIGDLLHLRYL 600
Query: 601 NITYCYIDFLPKAVTKLYHLETLIIRGCLELRELSSDIKNLINLRHLDIKDFKHVWSYMP 660
+++ LP+ + KL +L+TL + C L L L +LRHL + D + S P
Sbjct: 601 DLSCNNFRSLPERLCKLQNLQTLDVHNCYSLNCLPKQTSKLSSLRHL-VVDGCPLTSTPP 660
Query: 661 KGMGSMTTLQTMNLFILGENKGGELSELNGLVNLRGSLSIQQLQFCKPIGLENVKYLEEK 720
+ +G +T L+T+ FI+G KG +L EL L NL GS+SI L+ K + L K
Sbjct: 661 R-IGLLTCLKTLGFFIVGSKKGYQLGELKNL-NLCGSISITHLERVKN-DTDAEANLSAK 720
Query: 721 SRIQKLELHWKTYQRESKIDDEDERVLESLKPHSNLQKIRIEGYRGLKLCNWFSFDSIVN 780
+ +Q L + W ++ + ++ +VLE+LKPH NL+ + I + G + +W + +
Sbjct: 721 ANLQSLSMSWDN-DGPNRYESKEVKVLEALKPHPNLKYLEIIAFGGFRFPSWINHSVLEK 780
Query: 781 LVFIKLFNCEKLQQLPRFDRFPFLKHLHLED-LPSIEYIAINNYVSS-SMTTFFPSLENL 840
++ +++ +C+ LP F P L++L L++ +EY+ ++ S S FPSL+ L
Sbjct: 781 VISVRIKSCKNCLCLPPFGELPCLENLELQNGSAEVEYVEEDDVHSRFSTRRSFPSLKKL 840
Query: 841 SIIKLPNLKEWWKGESIDQNTSFPTILRHLSQLKIHYCRQLASIPQHGPLQSLDIRDISL 900
I +LK K E ++ FP L ++ I YC L P ++ L++
Sbjct: 841 RIWFFRSLKGLMKEEGEEK---FPM----LEEMAILYC-PLFVFPTLSSVKKLEVHG--- 900
Query: 901 QLFELVIKMTATNIIFLPNDLFSNVTHLQSLVIGRCFNLKMSFDDDNVRWKELGSLRTLR 960
TN L + SN++ L SL IG + ++ + L +L L
Sbjct: 901 ----------NTNTRGLSS--ISNLSTLTSLRIGANYRATSLPEE---MFTSLTNLEFLS 960
Query: 961 LCFIPKLEYLPKGFQYLKALEHLELLWCENLACI--LGIEHLTSLSRLEISNCPNLTSLP 1020
L+ LP L AL+ L++ C++L G+E LTSL++L + C L LP
Sbjct: 961 FFDFKNLKDLPTSLTSLNALKRLQIESCDSLESFPEQGLEGLTSLTQLFVKYCKMLKCLP 964
Query: 1021 EGMTQLISLTCLIIDDCPNL 1034
EG+ L +LT L + CP +
Sbjct: 1021 EGLQHLTALTNLGVSGCPEV 964
BLAST of Cucsa.328080 vs. Swiss-Prot
Match:
R13L1_ARATH (Putative disease resistance RPP13-like protein 1 OS=Arabidopsis thaliana GN=RPPL1 PE=3 SV=1)
HSP 1 Score: 435.6 bits (1119), Expect = 1.5e-120
Identity = 302/999 (30.23%), Postives = 509/999 (50.95%), Query Frame = 1
Query: 35 LDKLKGTVSALEAVLLDAEEKQSKSRAVKDWILKLKDTFYDIDDLLDVFSYESLKRQVMT 94
L++L + + AVL+DAEEKQ + V+ W+ +L+D Y +D LD + E+L+ +
Sbjct: 39 LERLSTALLTITAVLIDAEEKQITNPVVEKWVNELRDVVYHAEDALDDIATEALRLNIGA 98
Query: 95 KHRTNNT-KKVRIFFSKSNQIAFRLK-MSQKIKRVREKLDAIAMDKTQFNLYENTREIQD 154
+ ++N +++R S + + + + ++++V +L+ +A + L E T I
Sbjct: 99 ESSSSNRLRQLRGRMSLGDFLDGNSEHLETRLEKVTIRLERLASQRNILGLKELTAMIPK 158
Query: 155 DESTKRLETTSFIREGEIIGRDDDKKSIIHYLLDTNIHEDSVAVIAIIGMGGLGKTALVQ 214
+RL TTS + E E+ GRDDDK I+ +L+ N ++ + V+AI+G+GG+GKT L Q
Sbjct: 159 ----QRLPTTSLVDESEVFGRDDDKDEIMRFLIPENGKDNGITVVAIVGIGGVGKTTLSQ 218
Query: 215 SIYGDEKVKKHFELTMWVCISEEFDVKVIIEKIIESLTKKKREPDLQLDTLQSMVREKID 274
+Y D+ V+ +F +W +SEEFDV I +K+ ES+T + E LD LQ ++E++
Sbjct: 219 LLYNDQHVRSYFGTKVWAHVSEEFDVFKITKKVYESVTSRPCE-FTDLDVLQVKLKERLT 278
Query: 275 GK--RYLLVMDDVWNVNRAKWISLKRYLMGGAKGSRILITTRTHQVAQTFETILSHHLKE 334
G +LLV+DD+WN N A W L++ + A+GS+IL+TTR+ +VA + H+L+
Sbjct: 279 GTGLPFLLVLDDLWNENFADWDLLRQPFIHAAQGSQILVTTRSQRVASIMCAVHVHNLQP 338
Query: 335 LDEEKSWKLFRKMAFSNESEVLENSKLVVIGKEIVTKLKGSPLAIRVIGSYLYSKKSEKD 394
L + W LF K F N+ L N ++ + + IV K +G PLA++ +G L + +
Sbjct: 339 LSDGDCWSLFMKTVFGNQEPCL-NREIGDLAERIVHKCRGLPLAVKTLGGVLRFEGKVIE 398
Query: 395 WLSFKDHELDTIMQQENEIQSILKISFNHLSSSLKHCFTYCALFSKDYHYEIRKNDLIKQ 454
W + + ++ + +L++S+ +L + LK CF YC++F K + +E K+ ++
Sbjct: 399 WERVLSSRIWDLPADKSNLLPVLRVSYYYLPAHLKRCFAYCSIFPKGHAFE--KDKVVLL 458
Query: 455 WMAQGFI-QPHNKKAMEDVGDDYFEELLGRSFFQDIRKNKWGEIKKFKMHDIIHDLA--- 514
WMA+GF+ Q + K +E++G++YF EL RS Q + ++ MHD I++LA
Sbjct: 459 WMAEGFLQQTRSSKNLEELGNEYFSELESRSLLQKTK-------TRYIMHDFINELAQFA 518
Query: 515 ----CSVVENDCVLANDDTKSIDKRTRHVSISAFNSMTRWKLITKSLIEAKNLRTL---- 574
S E+ C L + +RTR++S N + ++L E K LRT
Sbjct: 519 SGEFSSKFEDGCKL------QVSERTRYLSYLRDNYAEPMEF--EALREVKFLRTFLPLS 578
Query: 575 --NYARRHHID-------LSNHLRLRTLNLEFHFV----PKCIGKMKHLRYINITYCYID 634
N +R +D L RLR L+L + + P + H R+++++ ++
Sbjct: 579 LTNSSRSCCLDQMVSEKLLPTLTRLRVLSLSHYKIARLPPDFFKNISHARFLDLSRTELE 638
Query: 635 FLPKAVTKLYHLETLIIRGCLELRELSSDIKNLINLRHLDIKDFKHVWSYMPKGMGSMTT 694
LPK++ +Y+L+TL++ C L+EL +DI NLINLR+LD+ K MP+ G + +
Sbjct: 639 KLPKSLCYMYNLQTLLLSYCSSLKELPTDISNLINLRYLDLIGTK--LRQMPRRFGRLKS 698
Query: 695 LQTMNLFILGENKGGELSELNGLVNLRGSLSIQQLQFCKPIGLENVKYLEEKSRIQKLEL 754
LQT+ F + + G +SEL GL +L G L I +LQ + L K +++++
Sbjct: 699 LQTLTTFFVSASDGSRISELGGLHDLHGKLKIVELQRVVDVADAAEANLNSKKHLREIDF 758
Query: 755 HWKTYQRESKIDDEDER------VLESLKPHSNLQKIRIEGYRGLKLCNWFSFDSIVNLV 814
W+T S+ + R V E L+PH +++K+ IE Y+G + +W S S +V
Sbjct: 759 VWRTGSSSSENNTNPHRTQNEAEVFEKLRPHRHIEKLAIERYKGRRFPDWLSDPSFSRIV 818
Query: 815 FIKLFNCEKLQQLPRFDRFPFLKHLHLEDLPSIEYIAINNYVSSSMTTF-----FPSLEN 874
I+L C+ LP + P LK LH+ + ++ I Y S F SLE
Sbjct: 819 CIRLRECQYCTSLPSLGQLPCLKELHISGMVGLQSIGRKFYFSDQQLRDQDQQPFRSLET 878
Query: 875 LSIIKLPNLKEW-----WKGESI------------DQNTSFPTILRHLSQLKIHYCRQLA 934
L LP+ +EW +G+ + + PT L L L I+ C L
Sbjct: 879 LRFDNLPDWQEWLDVRVTRGDLFPSLKKLFILRCPELTGTLPTFLPSLISLHIYKCGLLD 938
Query: 935 SIPQHGPLQSLDIRDISLQLFELVIKMTATNIIFLPNDLFSNVTHLQSLVIGRCFNLKMS 971
P H +++ +S IK + ++ P + F+N L L + +C +L S
Sbjct: 939 FQPDHHEYSYRNLQTLS-------IKSSCDTLVKFPLNHFAN---LDKLEVDQCTSL-YS 998
BLAST of Cucsa.328080 vs. TrEMBL
Match:
A0A0A0KIT2_CUCSA (Uncharacterized protein OS=Cucumis sativus GN=Csa_6G375730 PE=3 SV=1)
HSP 1 Score: 1401.0 bits (3625), Expect = 0.0e+00
Identity = 701/775 (90.45%), Postives = 714/775 (92.13%), Query Frame = 1
Query: 324 LSHHLKELDEEKSWKLFRKMAFSNESEVL--ENSKLVVIG------------KEIVTKLK 383
L + +E+ +++S K +F E E++ ++ K +I EIVTKLK
Sbjct: 143 LYENTREIQDDESTKRLETTSFIREGEIIGRDDDKKSIIHYLLDTNIHEDSVAEIVTKLK 202
Query: 384 GSPLAIRVIGSYLYSKKSEKDWLSFKDHELDTIMQQENEIQSILKISFNHLSSSLKHCFT 443
GSPLAIRVIGSYLYSKKSEKDWLSFKDHELDTIMQQENEIQSILKISFNHLSSSLKHCFT
Sbjct: 203 GSPLAIRVIGSYLYSKKSEKDWLSFKDHELDTIMQQENEIQSILKISFNHLSSSLKHCFT 262
Query: 444 YCALFSKDYHYEIRKNDLIKQWMAQGFIQPHNKKAMEDVGDDYFEELLGRSFFQDIRKNK 503
YCALFSKDYH+EIRKNDLIKQWMAQGFIQPHNKKAMEDVGDDYFEELLGRSFFQDIRKNK
Sbjct: 263 YCALFSKDYHHEIRKNDLIKQWMAQGFIQPHNKKAMEDVGDDYFEELLGRSFFQDIRKNK 322
Query: 504 WGEIKKFKMHDIIHDLACSVVENDCVLANDDTKSIDKRTRHVSISAFNSMTRWKLITKSL 563
WGEI KFKMHDIIHDLACSVVENDCVLANDDTKSIDKRTRHVSISAFNSMTRWKLITKSL
Sbjct: 323 WGEINKFKMHDIIHDLACSVVENDCVLANDDTKSIDKRTRHVSISAFNSMTRWKLITKSL 382
Query: 564 IEAKNLRTLNYARRHHIDLSNHLRLRTLNLEFHFVPKCIGKMKHLRYINITYCYIDFLPK 623
IEAKNLRTLNYARRHHIDLSNHLRLRTLNLEFHFVPKCIGKMKHLRYINITYCYIDFLPK
Sbjct: 383 IEAKNLRTLNYARRHHIDLSNHLRLRTLNLEFHFVPKCIGKMKHLRYINITYCYIDFLPK 442
Query: 624 AVTKLYHLETLIIRGCLELRELSSDIKNLINLRHLDIKDFKHVWSYMPKGMGSMTTLQTM 683
AVTKLYHLETLIIRGCLELRELSSDIKNLINLRHLDIKDFKHVWSYMPKGMGSMTTLQTM
Sbjct: 443 AVTKLYHLETLIIRGCLELRELSSDIKNLINLRHLDIKDFKHVWSYMPKGMGSMTTLQTM 502
Query: 684 NLFILGENKGGELSELNGLVNLRGSLSIQQLQFCKPIGLENVKYLEEKSRIQKLELHWKT 743
NLFILGENKGGELSELNGLVNLRGSLSIQQLQFCKPIGLENVKYLEEKSRIQKLELHWKT
Sbjct: 503 NLFILGENKGGELSELNGLVNLRGSLSIQQLQFCKPIGLENVKYLEEKSRIQKLELHWKT 562
Query: 744 YQRESKIDDEDERVLESLKPHSNLQKIRIEGYRGLKLCNWFSFDSIVNLVFIKLFNCEKL 803
YQRESKIDDEDERVLESLKPHSNLQKIRIEGYRGLKLCNWFSFDSIVNLVFIKLFNCEKL
Sbjct: 563 YQRESKIDDEDERVLESLKPHSNLQKIRIEGYRGLKLCNWFSFDSIVNLVFIKLFNCEKL 622
Query: 804 QQLPRFDRFPFLKHLHLEDLPSIEYIAINNYVSSSMTTFFPSLENLSIIKLPNLKEWWKG 863
QQLPRFDRFPFLKHLHLEDLPSIEYIAINNYVSSSMTTFFPSLENLSIIKLPNLKEWWKG
Sbjct: 623 QQLPRFDRFPFLKHLHLEDLPSIEYIAINNYVSSSMTTFFPSLENLSIIKLPNLKEWWKG 682
Query: 864 ESIDQNTSFPTILRHLSQLKIHYCRQLASIPQHGPLQSLDIRDISLQLFELVIKMTATNI 923
ESIDQNTSFPTILRHLSQLKIHYCRQLASIPQHGPLQSLDIRDISLQLFELVIKMTATNI
Sbjct: 683 ESIDQNTSFPTILRHLSQLKIHYCRQLASIPQHGPLQSLDIRDISLQLFELVIKMTATNI 742
Query: 924 I----------------------------FLPNDLFSNVTHLQSLVIGRCFNLKMSFDDD 983
I FLPNDLFSNVTHLQSLVIGRCFNLKMSFDDD
Sbjct: 743 IVGEDSSSSANDMFIRSSSSLKIWKIDWEFLPNDLFSNVTHLQSLVIGRCFNLKMSFDDD 802
Query: 984 NVRWKELGSLRTLRLCFIPKLEYLPKGFQYLKALEHLELLWCENLACILGIEHLTSLSRL 1043
NVRWKELGSLRTLRLCFIPKLEYLPKGFQYLKALEHLELLWCENLACILGIEHLTSLSRL
Sbjct: 803 NVRWKELGSLRTLRLCFIPKLEYLPKGFQYLKALEHLELLWCENLACILGIEHLTSLSRL 862
Query: 1044 EISNCPNLTSLPEGMTQLISLTCLIIDDCPNLSTLPEGLHHLLNTPRYAPLIFSH 1057
EISNCPNLTSLPEGMTQLISLTCLIIDDCPNLSTLPEGLHHLLNTPRYAPLIFSH
Sbjct: 863 EISNCPNLTSLPEGMTQLISLTCLIIDDCPNLSTLPEGLHHLLNTPRYAPLIFSH 917
BLAST of Cucsa.328080 vs. TrEMBL
Match:
A0A0A0KIT2_CUCSA (Uncharacterized protein OS=Cucumis sativus GN=Csa_6G375730 PE=3 SV=1)
HSP 1 Score: 383.3 bits (983), Expect = 9.8e-103
Identity = 207/252 (82.14%), Postives = 216/252 (85.71%), Query Frame = 1
Query: 1 MAESILCSLAGSIITKLGSFALQDLGLLWGFHDELDKLKGTVSALEAVLLDAEEKQSKSR 60
MAESILCSLAGSIITKLGSFALQDLGLLWGFHDELDKLKGTVSALEAVLLDAEEKQSKSR
Sbjct: 1 MAESILCSLAGSIITKLGSFALQDLGLLWGFHDELDKLKGTVSALEAVLLDAEEKQSKSR 60
Query: 61 AVKDWILKLKDTFYDIDDLLDVFSYESLKRQVMTKHRTNNTKKVRIFFSKSNQIAFRLKM 120
AVKDWILKLKDTFYDIDDLLDVFSYESLKRQVMTKHRTNNTKKVRIFFSKSNQIAFRLKM
Sbjct: 61 AVKDWILKLKDTFYDIDDLLDVFSYESLKRQVMTKHRTNNTKKVRIFFSKSNQIAFRLKM 120
Query: 121 SQKIKRVREKLDAIAMDKTQFNLYENTREIQDDESTKRLETTSFIREGEIIGRDDDKKSI 180
SQKIKRVREKLDAIAMDKTQFNLYENTREIQDDESTKRLETTSFIREGEIIGRDDDKKSI
Sbjct: 121 SQKIKRVREKLDAIAMDKTQFNLYENTREIQDDESTKRLETTSFIREGEIIGRDDDKKSI 180
Query: 181 IHYLLDTNIHEDSVAVIAIIGMGGLGKTALVQSIYGDEKVKKHFELTMWVCISE-EFDVK 240
IHYLLDTNIHEDSVA I G ++ S +K +K W+ + E D
Sbjct: 181 IHYLLDTNIHEDSVAEIVTKLKGSPLAIRVIGSYLYSKKSEKD-----WLSFKDHELDTI 240
Query: 241 VIIEKIIESLTK 252
+ E I+S+ K
Sbjct: 241 MQQENEIQSILK 247
HSP 2 Score: 876.7 bits (2264), Expect = 2.8e-251
Identity = 471/707 (66.62%), Postives = 527/707 (74.54%), Query Frame = 1
Query: 351 VLENSKLVVIGKEIVTKLKGSPLAIRVIGSYLYSKKSEKDWLSFKDHELDTIMQQENEIQ 410
+LENSKLV IGKEIV KLKGSPLAIRVIGSYLYSKKSEKDWLSFK++ELDTIMQQ+NEIQ
Sbjct: 1 MLENSKLVGIGKEIVAKLKGSPLAIRVIGSYLYSKKSEKDWLSFKENELDTIMQQKNEIQ 60
Query: 411 SILKISFNHLSSSLKHCFTYCALFSKDYHYEIRKNDLIKQWMAQGFIQPHNKKAMEDVGD 470
SILKISFNHLSSSLK CFTYCALF KD+ EI K+DLIKQWM +GFIQPHNKKAMEDVGD
Sbjct: 61 SILKISFNHLSSSLKQCFTYCALFLKDF--EIDKDDLIKQWMGEGFIQPHNKKAMEDVGD 120
Query: 471 DYFEELLGRSFFQDIRKNKWGEIKKFKMHDIIHDLACSVVENDCVLANDDTKSIDKRTRH 530
+YF+ELLGRSFFQDI KN+ GEI KFKMHD +HDLAC V END V A DDTK IDKRTRH
Sbjct: 121 EYFKELLGRSFFQDISKNQLGEIMKFKMHDFMHDLACFVGENDYVFATDDTKFIDKRTRH 180
Query: 531 VSISAFNSMTRWKLITKSLIEAKNLRTLNYARRHH------IDLSNHLRLRTLNLEFH-F 590
+SIS F S TRW++I +SLI AKNLRTLNYA ++ ID SNHLRLRTLNL F
Sbjct: 181 LSISPFISKTRWEVIKESLIAAKNLRTLNYACHNYDGDEIEIDFSNHLRLRTLNLIFSTH 240
Query: 591 VPKCIGKMKHLRYINITYCYIDFLPKAVTKLYHLETLIIRGCLELRELSSDIKNLINLRH 650
VPKCIGKMKHLRYIN T CY DFLPK VTKLYHLETLI R C +LREL SDI NLINLRH
Sbjct: 241 VPKCIGKMKHLRYINFTRCYFDFLPKVVTKLYHLETLIFRECFKLRELPSDITNLINLRH 300
Query: 651 LDIKDFKHVWSYMPKGMGSMTTLQTMNLFILGENKGGELSELNGLVNLRGSLSIQQLQFC 710
L I SYMPKGMGSMTTLQT+NLFILGEN+GGELSELNGL+NLRGSLSIQQLQFC
Sbjct: 301 LGINSLIEGLSYMPKGMGSMTTLQTLNLFILGENEGGELSELNGLINLRGSLSIQQLQFC 360
Query: 711 KPIGLENVKYLEEKSRIQKLELHWKTYQRESKIDDEDERVLESLKPHSNLQKIRIEGYRG 770
KPIG+E+ K+LEEKS IQKL+L+W +R+ +IDDEDE+VLE LKPH NLQKI I GY G
Sbjct: 361 KPIGIESAKHLEEKSGIQKLKLYWYHLERKYEIDDEDEKVLECLKPHPNLQKIVINGYGG 420
Query: 771 LKLCNWFSFDSIVNLVFIKLFNCEKLQQLPRFDRFPFLKHLHLEDLPSIEYIAINNYVSS 830
+KLCNWFSFD IVNLV I LFNC KLQQLPRFD+FPFLKHL L+ LP++E+I N+ VSS
Sbjct: 421 VKLCNWFSFDYIVNLVIIDLFNCNKLQQLPRFDQFPFLKHLKLQYLPNVEFIDNNDSVSS 480
Query: 831 SMTTFFPSLENLSIIKLPNLKEWWKGESIDQNTSFPTILRHLSQLKIHYCRQLASIPQHG 890
S+TTFFPSLE L I +LP LKEWWK + IDQ +IPQH
Sbjct: 481 SLTTFFPSLEKLRIFRLPKLKEWWKRKLIDQ-----------------------TIPQHR 540
Query: 891 PLQSLDIRDISLQLFELVIKMTATNIIFLPNDLFSNVT---------------------- 950
L+SL+I +SLQ+FELV++M TNII D S+ T
Sbjct: 541 RLESLNISGVSLQVFELVMEMATTNIIVGSQDSSSSTTSISLSFLSIEDIDFEFLQFHDL 600
Query: 951 -----HLQSLVIGRCFNLKMSFDDDNVRWKELGSLRTLRLCFIPKLEYLPKGFQYLKALE 1010
HL+SL I C N+KMS D V WK LGSLR L L IP LEYLPK Q + L+
Sbjct: 601 FSNMTHLKSLWIINCKNIKMSSSLDAVTWKGLGSLRELMLSSIPDLEYLPKSLQCVTTLQ 660
Query: 1011 HLELLWCENLACILGIEHL-TSLSRLEISNCPNLTSLPEGMTQLISL 1023
L++ C NL I I HL TSLS LEI CPN+T P M+QL SL
Sbjct: 661 SLQIYNCPNLVSIESIRHLTTSLSVLEIHGCPNITFYPHEMSQLASL 682
BLAST of Cucsa.328080 vs. TrEMBL
Match:
A0A0A0K5Z6_CUCSA (Uncharacterized protein OS=Cucumis sativus GN=Csa_7G425920 PE=4 SV=1)
HSP 1 Score: 733.8 bits (1893), Expect = 3.0e-208
Identity = 388/691 (56.15%), Postives = 498/691 (72.07%), Query Frame = 1
Query: 390 DWLSFKDHELDTIMQQENEIQSILKISFNHLSSSLKHCFTYCALFSKDYHYEIRKNDLIK 449
DWLSFKD++L TI+QQEN+IQ ILKISFNHL S+LKHCFTYCALF KDY E +K+ L+K
Sbjct: 2 DWLSFKDNDLGTILQQENQIQPILKISFNHLPSNLKHCFTYCALFPKDY--EFQKDGLVK 61
Query: 450 QWMAQGFIQPHNKKAMEDVGDDYFEELLGRSFFQDIRKNKWGEIKKFKMHDIIHDLACSV 509
QWMAQGFIQ H+ K +EDVGDDYF+ELLGRSFF +++ NKWG++K+ KMHD+IHDLAC +
Sbjct: 62 QWMAQGFIQSHSNKEIEDVGDDYFKELLGRSFFHNVKVNKWGDVKECKMHDLIHDLACWI 121
Query: 510 VENDCVLANDDTKSIDKRTRHVSISAFNSMTRWKLITKSLIEAKNLRTLNYARRHHIDLS 569
VEN+CV A+D TKSIDKRTRHVS + S W+L KSL E KNLRTL+ +
Sbjct: 122 VENECVDASDKTKSIDKRTRHVSFPSNYSRKSWELEAKSLTEVKNLRTLH--GPPFLLSE 181
Query: 570 NHLRLRTLNL---EFHFVPKCIGKMKHLRYINITYCYIDFLPKAVTKLYHLETLIIRGCL 629
NHLRLR+LNL +F +PK I +++HLRY++I+ + FLPK +TKLY+LETLI+R C
Sbjct: 182 NHLRLRSLNLGYSKFQKIPKFISQLRHLRYLDISDHDMKFLPKFITKLYNLETLILRHCS 241
Query: 630 ELRELSSDIKNLINLRHLDIKDFKHVWSYMPKGMGSMTTLQTMNLFILGENKGGELSELN 689
+LREL +DI NLINL+HLD+ + ++MPKG+G +T+LQTMNLF+LG++KG +LSELN
Sbjct: 242 DLRELPTDINNLINLKHLDVHGCYRL-THMPKGLGGLTSLQTMNLFVLGKDKGCDLSELN 301
Query: 690 GLVNLRGSLSIQQLQFCKPIGLENVKYLEEKSRIQKLELHWKTYQRESKID----DEDER 749
L LRGSL I+ L+ C L+N KY+EEK IQKL+L W +++ D ++DER
Sbjct: 302 ELARLRGSLLIKGLELCTTTDLKNAKYMEEKFGIQKLKLRWNRDLYDAETDYASENDDER 361
Query: 750 VLESLKPHSNLQKIRIEGYRGLKLCNWFSFDSIVNLVFIKLFNCEKLQQLPRFDRFPFLK 809
VL+ LKPHSN+ K++I GYRG+KLCNW SFD + LV I+L +CEKLQ LP+FD+FPFLK
Sbjct: 362 VLDCLKPHSNVHKMQIRGYRGVKLCNWLSFDYLGGLVNIELQSCEKLQHLPQFDQFPFLK 421
Query: 810 HLHLEDLPSIEYIAINNYVSSSMTTFFPSLENLSIIKLPNLKEWWKG----ESIDQNTSF 869
HL LE+LPSIEYI NN +SSS TFFPSLE L+I+ +PNLK WWKG ES + F
Sbjct: 422 HLLLENLPSIEYIDNNNSLSSS--TFFPSLEKLTIMTMPNLKGWWKGETPPESARYSALF 481
Query: 870 PTILRHLSQLKIHYCRQLASIPQHGPLQSLDIRDISLQLFELVIKMTAT----------- 929
PTIL HLS+L I C QLASIPQH PL+SL + D+S+QLF++VIKM T
Sbjct: 482 PTILHHLSRLDISNCPQLASIPQHPPLRSLALNDVSVQLFDMVIKMATTPAADSSSALSK 541
Query: 930 ---------NIIFLPNDLFSNVTHLQSLVIGRCFNLKMSF------DDDNVRWKELGSLR 989
++ FLP +LF + T L+ + C NL+MS D+D V K+LG+L
Sbjct: 542 LSILHIQNIDLEFLPEELFGSTTDLEIFTVVNCKNLQMSSSHLVDEDNDGVLGKKLGNLH 601
Query: 990 TLRLCFIPKLEYLPKGFQYLKALEHLELLWCENLACILGIEHLTSLSRLEISNCPNLTSL 1044
+L + +P+LEYL K +Y+ LE L+L C N+ + GI HLTSLS L I NC NLTSL
Sbjct: 602 SLGIFDMPQLEYLWKELKYMTTLERLDLYNCPNIVSLEGISHLTSLSSLRICNCSNLTSL 661
BLAST of Cucsa.328080 vs. TrEMBL
Match:
A0A0A0K5Z6_CUCSA (Uncharacterized protein OS=Cucumis sativus GN=Csa_7G425920 PE=4 SV=1)
HSP 1 Score: 94.7 bits (234), Expect = 7.0e-16
Identity = 82/278 (29.50%), Postives = 131/278 (47.12%), Query Frame = 1
Query: 781 IKLFNCEKLQQLPRFDRFPFLKHLHLEDLPSIEYIAINNYVSSSMTTFFPSLENLSIIKL 840
+ + NC +L +P+ P L+ L L D+ + + ++ +L LSI+ +
Sbjct: 484 LDISNCPQLASIPQH---PPLRSLALNDVSVQLFDMVIKMATTPAADSSSALSKLSILHI 543
Query: 841 PNLKEWWKGESIDQNTSFPTILRHLSQLKIHYCRQLASIPQH--------------GPLQ 900
N+ + E + +T+ L + C+ L H G L
Sbjct: 544 QNIDLEFLPEELFGSTT------DLEIFTVVNCKNLQMSSSHLVDEDNDGVLGKKLGNLH 603
Query: 901 SLDIRDISLQLFELVIKMTATNIIFLPNDLFSNVTHLQSLVIGRCFNLKMSFDDDNVRWK 960
SL I D+ + +L +L +T L+ L + C N+ +S + +
Sbjct: 604 SLGIFDMP-------------QLEYLWKEL-KYMTTLERLDLYNCPNI-VSLEGIS---- 663
Query: 961 ELGSLRTLRLCFIPKLEYLPKGFQYLKALEHLELLWCENLACI-LGIEHLTSLSRLEISN 1020
L SL +LR+C L LP+G +L +L +L ++ C NL + GI HLTSLS L I
Sbjct: 664 HLTSLSSLRICNCSNLTSLPEGISHLTSLSYLTIVCCPNLTSLPAGIGHLTSLSTLLIKY 723
Query: 1021 CPNLTSLPEGMTQLISLTCLIIDDCPNLSTLPEGLHHL 1044
C NLTSLPEG++ L SL+ I++CP L++LPEG+ HL
Sbjct: 724 CVNLTSLPEGVSHLTSLSSFTIEECPCLTSLPEGVSHL 733
HSP 2 Score: 89.4 bits (220), Expect = 2.9e-14
Identity = 59/137 (43.07%), Postives = 77/137 (56.20%), Query Frame = 1
Query: 908 NIIFLPNDLFSNVTHLQSLVIGRCFNLKMSFDDDNVRWKELGSLRTLRLCFIPKLEYLPK 967
NI+ L S++T L SL I C NL + + L SL L + P L LP
Sbjct: 627 NIVSLEG--ISHLTSLSSLRICNCSNLTSLPEGIS----HLTSLSYLTIVCCPNLTSLPA 686
Query: 968 GFQYLKALEHLELLWCENLACIL-GIEHLTSLSRLEISNCPNLTSLPEGMTQLISLTCLI 1027
G +L +L L + +C NL + G+ HLTSLS I CP LTSLPEG++ L SL+ LI
Sbjct: 687 GIGHLTSLSTLLIKYCVNLTSLPEGVSHLTSLSSFTIEECPCLTSLPEGVSHLTSLSTLI 746
Query: 1028 IDDCPNLSTLPEGLHHL 1044
I C NL++LPEG+ HL
Sbjct: 747 IRRCVNLTSLPEGIGHL 757
HSP 3 Score: 88.6 bits (218), Expect = 5.0e-14
Identity = 84/273 (30.77%), Postives = 119/273 (43.59%), Query Frame = 1
Query: 772 FDSIVNLVFIKLFNCEKLQQLPRFDRFPFLKHLHLEDLPSIEYIAINNYVSSSMTTFFPS 831
F S +L + NC+ LQ HL ED + + N
Sbjct: 553 FGSTTDLEIFTVVNCKNLQMSS--------SHLVDEDNDGVLGKKLGN------------ 612
Query: 832 LENLSIIKLPNLKEWWKGESIDQNTSFPTILRHLSQLKIHYCRQLASIPQHGPLQSLDIR 891
L +L I +P L+ WK + L +L ++ C + S+ L SL
Sbjct: 613 LHSLGIFDMPQLEYLWKELKY---------MTTLERLDLYNCPNIVSLEGISHLTSLS-- 672
Query: 892 DISLQLFELVIKMTATNIIFLPNDLFSNVTHLQSLVIGRCFNLKMSFDDDNVRWKELGSL 951
SL++ +N+ LP + S++T L L I C NL L SL
Sbjct: 673 --SLRI------CNCSNLTSLPEGI-SHLTSLSYLTIVCCPNLTSL----PAGIGHLTSL 732
Query: 952 RTLRLCFIPKLEYLPKGFQYLKALEHLELLWCENLACIL-GIEHLTSLSRLEISNCPNLT 1011
TL + + L LP+G +L +L + C L + G+ HLTSLS L I C NLT
Sbjct: 733 STLLIKYCVNLTSLPEGVSHLTSLSSFTIEECPCLTSLPEGVSHLTSLSTLIIRRCVNLT 781
Query: 1012 SLPEGMTQLISLTCLIIDDCPNLSTLPEGLHHL 1044
SLPEG+ L SL+ I++C NL++LPEGL HL
Sbjct: 793 SLPEGIGHLTSLSIFTIEECLNLTSLPEGLLHL 781
HSP 4 Score: 38.9 bits (89), Expect = 4.6e+01
Identity = 34/121 (28.10%), Postives = 60/121 (49.59%), Query Frame = 1
Query: 548 SLIEAKNLRTLNYARRHHIDLSNHLRLRTLNLEFHFVPKCIGKMKHLRYINITYC-YIDF 607
+++ NL +L H LS L +NL +P+ + + L I C +
Sbjct: 668 TIVCCPNLTSLPAGIGHLTSLSTLLIKYCVNLTS--LPEGVSHLTSLSSFTIEECPCLTS 727
Query: 608 LPKAVTKLYHLETLIIRGCLELRELSSDIKNLINLRHLDIKDFKHVWSYMPKGMGSMTTL 667
LP+ V+ L L TLIIR C+ L L I +L +L I++ ++ S +P+G+ +++L
Sbjct: 728 LPEGVSHLTSLSTLIIRRCVNLTSLPEGIGHLTSLSIFTIEECLNLTS-LPEGLLHLSSL 785
HSP 5 Score: 731.9 bits (1888), Expect = 1.1e-207
Identity = 453/1094 (41.41%), Postives = 651/1094 (59.51%), Query Frame = 1
Query: 1 MAESILCSLAGSIITKLGSFAL-QDLGLLWGFHDELDKLKGTVSALEAVLLDAEEKQSKS 60
MAE+IL ++A SII KLGS L +++GLL+G + EL KLK TVSA++AVLLDAE+KQ+++
Sbjct: 1 MAEAILFNVAESIIEKLGSETLGKEMGLLFGVNTELQKLKRTVSAIKAVLLDAEQKQAEN 60
Query: 61 RAVKDWILKLKDTFYDIDDLLDVFSYESLKRQVMTKHRTNNTKKVRIFFSKSNQIAFRLK 120
VKDW+ KL+D Y DDL+D F E+L+R+VM + KKVRIFFS SNQ+AF LK
Sbjct: 61 HQVKDWLQKLQDAVYYADDLVDEFYTEALQRRVMIG--SGKAKKVRIFFSSSNQLAFMLK 120
Query: 121 MSQKIKRVREKLDAIAMDKTQFNLYENTREIQDDESTKRLETTSFIREGEIIGRDDDKKS 180
+ KIK VRE L+ I D F L E + + S+ ET S++RE E+IGRD DK +
Sbjct: 121 IGHKIKEVRETLNEIK-DNKDFYLMERLEDTR--VSSMARETHSYVREEEVIGRDADKLA 180
Query: 181 IIHYLLDTNIHEDSVAVIAIIGMGGLGKTALVQSIYGDEKVKKHFELTMWVCISEEFDVK 240
I+ LLD ED V+VI I+G+GGLGKT L Q I+ DE ++KHFEL WVC+S+ FD+K
Sbjct: 181 IMKLLLDLESKED-VSVIPIVGIGGLGKTTLAQHIFNDENIQKHFELRSWVCVSDFFDLK 240
Query: 241 VIIEKIIESLTKKKREPDLQLDTLQSMVREKIDGKRYLLVMDDVWNVNRAKWISLKRYLM 300
+I+EKII+S+T K +L+L+ LQ +R++IDGKRYLLV+DDVWN + KW L+R LM
Sbjct: 241 LIVEKIIKSVTLKS-SGNLELEQLQFHLRKEIDGKRYLLVLDDVWNEDGEKWRRLERLLM 300
Query: 301 GGAKGSRILITTRTHQVAQTFETILSHHLKELDEEKSWKLFRKMAFSNESEVLENSKLVV 360
GGAKGSR+L+TTR++ VA+ TI + L LD SW LF+++AF E ENS ++
Sbjct: 301 GGAKGSRVLVTTRSYMVAKITHTIQPYKLSGLDNVDSWSLFKRIAFDKGQEP-ENSTVLA 360
Query: 361 IGKEIVTKLKGSPLAIRVIGSYLYSKKSEKDWLSFKDHELDTIMQQENEIQSILKISFNH 420
+G EIV K +G PLAI+ IGS LY K+ E +W SF + EL I Q E++I LK+S+NH
Sbjct: 361 MGMEIVEKCRGVPLAIKTIGSILYFKRREIEWSSFMETELSKIPQGEDDILPALKLSYNH 420
Query: 421 LSSSLKHCFTYCALFSKDYHYEIRKNDLIKQWMAQGFIQPHNK-KAMEDVGDDYFEELLG 480
L S LKHCF YC LF KD+ +++K LI+ W+AQGFI+P ++ + +EDVG +YF LL
Sbjct: 421 LPSHLKHCFAYCRLFPKDHEIDVQK--LIRLWIAQGFIKPSDRSQCLEDVGYEYFMALLW 480
Query: 481 RSFFQDIRKNKWGEIKKFKMHDIIHDLACSVVENDCVLANDDTKSIDKRTRHVSI-SAFN 540
RSFFQD + + WG +K+F++HD++HDLA V + + ++ ++ RHVS S FN
Sbjct: 481 RSFFQDPKIDDWGNVKRFRIHDLMHDLAVLVAGTSSISIDRSMENSNENIRHVSFDSKFN 540
Query: 541 SMTRWKL---------ITKSLIEAK--------------NLRTLNYARRHHIDLSNHLRL 600
WKL I ++ I K + + L + R I L + L
Sbjct: 541 Q--SWKLPPSLLKKNNIVRTFISPKYNAWHGYQHNFLGTSRKKLRWLTRDAI-LFSFTSL 600
Query: 601 RTLNLE---FHFVPKCIGKMKHLRYINITYCYIDFLPKAVTKLYHLETLIIRGCLELREL 660
RTL+L+ +P +G++KHLRY++++ I LP ++T+L +L+TLI+ C EL L
Sbjct: 601 RTLDLKALNIKKLPNSLGRLKHLRYLDVSRNNIQMLPNSITRLQNLQTLILSHCRELIRL 660
Query: 661 SSDIKNLINLRHLDIKDFKHVWSYMPKGMGSMTTLQTMNLFILGE-----NKGGELSELN 720
D+K L+NLRHL + + SYMP+G+G +T L +++ F++ + + LSEL
Sbjct: 661 PEDMKKLVNLRHLQTHGCRRL-SYMPRGIGELTALHSLDRFVVADTGNVTRRTSGLSELG 720
Query: 721 GLVNLRGSLSIQQLQFCKPIGLE-NVKYLEEKSRIQKLELHWK--TYQRESKIDDEDERV 780
GL NL LSI+ L K I LE ++K +Q L L W+ ++ + + DE
Sbjct: 721 GLNNLGAELSIENLGHGKNIALEVKAANFKDKQHLQSLVLFWRYGFDGDQTSVVENDEMS 780
Query: 781 LESLKPHSNLQKIRIEGYRGLKLCNWFSFDSIVNLVFIKLFNCEKLQQLPRFDRFPFLKH 840
L+ L+PHS ++ +++ + GL+ W S ++ NLV ++L +CEK Q LP P LK
Sbjct: 781 LDDLRPHSKMKALKVVNFMGLRFAGWLS--TLNNLVKLELLSCEKCQHLPHLHELPLLKE 840
Query: 841 LHLEDLPSIEYIA---INNYVSSSMTT----FFPSLENLSIIKLPNLKEWWKGESIDQNT 900
L L L ++EYI+ NN +S+S++T FFPSL L I NLK WW+ +I +
Sbjct: 841 LTLYALIALEYISDMESNNQLSTSLSTPTTPFFPSLTILKIRFCLNLKGWWRRSTIAGDN 900
Query: 901 S---------FPTILRH------LSQLKIHYCRQLASIPQHGPLQSLDIRDISLQLFELV 960
S P + ++ L L+I C L S+P L+SL++ D S + +
Sbjct: 901 SGALGLTTATTPPLHQNQPVFPCLHDLEIRNCPHLTSMPLFPDLKSLELMDTSFRPLQQT 960
Query: 961 IKMTATNIIFLPNDLFSNVTHLQSLVIGRCFNLKMSFDDDNVRWKELGSLRTLRLCFIPK 1020
+ MT ++I P+ + S S L L+ L + I
Sbjct: 961 MMMTDASMIASPSTIMSTPPDSSS---------------------SLSKLKNLLISQIKD 1020
Query: 1021 LEYLPKGFQYLKALEHLELLWCENLACILG-IEHLTSLSRLEISNCPNLTSLPEGMTQLI 1035
+E LP+G L +L+ L+++ C NL + I +L L L++ CP L SLPEGM L
Sbjct: 1021 VESLPEGIGNLTSLQSLDIVECPNLTSLPEVIGNLCLLQSLKVWECPKLESLPEGMCLLT 1057
BLAST of Cucsa.328080 vs. TrEMBL
Match:
W9R509_9ROSA (Putative disease resistance protein RGA4 OS=Morus notabilis GN=L484_023797 PE=3 SV=1)
HSP 1 Score: 43.5 bits (101), Expect = 1.8e+00
Identity = 23/49 (46.94%), Postives = 32/49 (65.31%), Query Frame = 1
Query: 995 LTSLSRLEISNCPNLTSLPEGMTQLISLTCLIIDDCPNLSTLPEGLHHL 1044
L+ L L IS ++ SLPEG+ L SL L I +CPNL++LPE + +L
Sbjct: 970 LSKLKNLLISQIKDVESLPEGIGNLTSLQSLDIVECPNLTSLPEVIGNL 1018
HSP 2 Score: 731.5 bits (1887), Expect = 1.5e-207
Identity = 442/1134 (38.98%), Postives = 656/1134 (57.85%), Query Frame = 1
Query: 1 MAESILCSLAGSIITKLGSFALQDLGLLWGFHDELDKLKGTVSALEAVLLDAEEKQSKSR 60
MAE+ L ++A I+ K+G+ ALQ++GL+WG ++L+KLK TVS ++AVLLDAE++ +KS
Sbjct: 1 MAEAGLFNIADGILGKIGNLALQEIGLVWGVKEQLEKLKNTVSTIKAVLLDAEDQHAKSH 60
Query: 61 AVKDWILKLKDTFYDIDDLLDVFSYESLKRQVMTKHRTNNTKKVRIFFSKSNQIAFRLKM 120
V+DW+ KLKD YD DDLLD FS LKRQ + K+V FSK +Q+ + LK+
Sbjct: 61 EVRDWLGKLKDAVYDTDDLLDNFSTHVLKRQ-----QGKRGKQVSFLFSKVSQVTYNLKI 120
Query: 121 SQKIKRVREKLDAIAMDKTQFNLYENTREIQDDESTKRLETTSFIREGEIIGRDDDKKSI 180
S +I+ +REKLDAIA DK +++ + + +R +T SF+R+ +++GR DK +I
Sbjct: 121 SHQIEAIREKLDAIAADKIKYHFTDRSPISIPLVKVERRQTHSFVRKEDVVGRQGDKDAI 180
Query: 181 IHYLLDTNIHEDSVAVIAIIGMGGLGKTALVQSIYGDEKVKKHFELTMWVCISEEFDVKV 240
+ LL++N D+V+VI I+G+GG GKT L Q Y DE+ KHFEL MWVC+S+ FDVK+
Sbjct: 181 MKRLLESN-GVDNVSVIPIVGIGGQGKTTLAQLAYNDERTVKHFELRMWVCVSDVFDVKM 240
Query: 241 IIEKIIESLTKKKREPDLQLDTLQSMVREKIDGKRYLLVMDDVWNVNRAKWISLKRYLMG 300
I+EKI+ES T K E L++D+LQ+ +R++IDG++YLLV+DD+WN +R +W++L LM
Sbjct: 241 IVEKILESATSSKYE-SLEMDSLQTHLRKRIDGRKYLLVLDDMWNDSRERWLNLADLLMN 300
Query: 301 GAKGSRILITTRTHQVAQTFETILSHHLKELDEEKSWKLFRKMAFSNESEVLENSKLVVI 360
GA+GS+I++TTR VA T + L+ L E+ SW LF K+AF ES+ +S+LV I
Sbjct: 301 GARGSKIIVTTRAQLVASITGTSQPYLLEGLPEDMSWSLFEKVAFK-ESKETNDSRLVAI 360
Query: 361 GKEIVTKLKGSPLAIRVIGSYLYSKKSEKDWLSFKDHELDTIMQQENEIQSILKISFNHL 420
GK+IV K G+PL IR IG LY+K +E +WLS K+ +L + + E+++ S+LK+S+ L
Sbjct: 361 GKDIVKKCAGNPLVIRTIGGVLYTKDTETEWLSLKERQLSMLTRNEDDVLSVLKLSYEQL 420
Query: 421 SSSLKHCFTYCALFSKDYHYEIRKNDLIKQWMAQGFIQP-HNKKAMEDVGDDYFEELLGR 480
S LK CF YC+LF KDY EI K LI W+A+GFIQP + +E++G YF +LL R
Sbjct: 421 PSYLKQCFAYCSLFPKDY--EINKQMLISLWIAEGFIQPLQGIQCLEELGGQYFMDLLRR 480
Query: 481 SFFQDIRKNKWGEIKKFKMHDIIHDLACSVVENDCVLANDDTKSIDKRTRHVSISAFNSM 540
SFFQD+ ++WG + K+HD++HDLA + +DC + + D ++I +RT HVS+SA
Sbjct: 481 SFFQDVEYDEWGNVISCKLHDLMHDLAQLIAGSDCSMVDLDCENISERTHHVSLSA-ELD 540
Query: 541 TRWKLITKSLIEAKNLRTLNYARR-----------HHIDLSNHLRLRTLNLE---FHFVP 600
+ WK+ T +L+ A +RT + H +S+ +R L+L H +P
Sbjct: 541 SSWKIPT-TLLNANKIRTFLLPMQPIHRVVLDKVDHEAIISSFRLMRVLDLHNTGLHILP 600
Query: 601 KCIGKMKHLRYINITYC-YIDFLPKAVTKLYHLETLIIRGCLELRELSSDIKNLINLRHL 660
+ IGK+KHLRY++++ I LP ++T+L +L+TL I C L +L + N+I+L+HL
Sbjct: 601 RIIGKLKHLRYLDLSKNEVIRKLPSSITELLNLQTLKIYSCKRLEQLPRKLSNMISLKHL 660
Query: 661 DIKDFKHVWSYMPKGMGSMTTLQTMNLFILG----ENKGGELSELNGLVNLRGSLSIQQL 720
+ + ++MP G+G +T+LQT+ F++G E G L EL L LRG L I +L
Sbjct: 661 ETGQCTGL-THMPSGIGQLTSLQTLTRFVVGMSSFEMASGGLRELKDLNELRGELMIAKL 720
Query: 721 QFCKPIGLE-NVKYLEEKSRIQKLELHWKTYQRESKIDDEDERVLESLKPHSNLQKIRIE 780
+ + + E L+EK ++ L L W +EDE +LE L+PHSNLQ+ I
Sbjct: 721 ENLRNVAAECEEANLKEKQHLEVLTLDWSREVNNHVSFEEDEALLEGLQPHSNLQEFHIY 780
Query: 781 GYRGLKLCNWFSFDSIV---NLVFIKLFNCEKLQQLPRFDRFPFLKHLHLEDLPSIEYIA 840
GYR + W SFD + NL+ I ++NC K LP F + P LK L LE + ++EYI
Sbjct: 781 GYRAERFPKWMSFDMALLLPNLLEITIWNCIKCIHLPLFSQLPKLKVLRLEVITAVEYIE 840
Query: 841 INNYVSSSMTTF--------------FPSLENLSIIKLPNLKEWWKG------------- 900
+ SSS +F FP LE L L NLK WW+
Sbjct: 841 DSRAESSSSLSFKGNPMNRGREGKEFFPCLEELVFFDLRNLKGWWREAPPVTNNNHGAAA 900
Query: 901 ------ESIDQNTSFPTILRHLSQLKIHYCRQLASIPQHGPLQSLDIRDISLQLFELVIK 960
+ + SFP LS+LKI C L +P H L+ L++++ +L +
Sbjct: 901 SPQRPLQKKESMVSFPC----LSKLKIGICTNLTHMPLHPFLEELELKNTPARLLQQSAM 960
Query: 961 MTA-TNIIF--------------------LPNDLFSNVTHLQSLVIGRCFNLKMSFDDDN 1020
+ A N+++ P ++T LQ L I C +L ++
Sbjct: 961 VAAGANLVYPLYLSKLKVMHIDGIIDLVSFPEKGIHHLTSLQHLSIENCPDLVCLTEEG- 1020
Query: 1021 VRWKELGSLRTLRLCFIPKLEYLPKGFQYLKALEHLELLWCENLACILGIEH----LTSL 1053
K L SLR + L+ L KGF++L ALE LE+ C L +E L L
Sbjct: 1021 --LKSLTSLRFFNIRCCEILKSLFKGFKHLTALEELEIKECRELDLSKDLEENVMELQCL 1080
BLAST of Cucsa.328080 vs. TAIR10
Match:
AT3G14470.1 (AT3G14470.1 NB-ARC domain-containing disease resistance protein)
HSP 1 Score: 435.6 bits (1119), Expect = 8.5e-122
Identity = 302/999 (30.23%), Postives = 509/999 (50.95%), Query Frame = 1
Query: 35 LDKLKGTVSALEAVLLDAEEKQSKSRAVKDWILKLKDTFYDIDDLLDVFSYESLKRQVMT 94
L++L + + AVL+DAEEKQ + V+ W+ +L+D Y +D LD + E+L+ +
Sbjct: 39 LERLSTALLTITAVLIDAEEKQITNPVVEKWVNELRDVVYHAEDALDDIATEALRLNIGA 98
Query: 95 KHRTNNT-KKVRIFFSKSNQIAFRLK-MSQKIKRVREKLDAIAMDKTQFNLYENTREIQD 154
+ ++N +++R S + + + + ++++V +L+ +A + L E T I
Sbjct: 99 ESSSSNRLRQLRGRMSLGDFLDGNSEHLETRLEKVTIRLERLASQRNILGLKELTAMIPK 158
Query: 155 DESTKRLETTSFIREGEIIGRDDDKKSIIHYLLDTNIHEDSVAVIAIIGMGGLGKTALVQ 214
+RL TTS + E E+ GRDDDK I+ +L+ N ++ + V+AI+G+GG+GKT L Q
Sbjct: 159 ----QRLPTTSLVDESEVFGRDDDKDEIMRFLIPENGKDNGITVVAIVGIGGVGKTTLSQ 218
Query: 215 SIYGDEKVKKHFELTMWVCISEEFDVKVIIEKIIESLTKKKREPDLQLDTLQSMVREKID 274
+Y D+ V+ +F +W +SEEFDV I +K+ ES+T + E LD LQ ++E++
Sbjct: 219 LLYNDQHVRSYFGTKVWAHVSEEFDVFKITKKVYESVTSRPCE-FTDLDVLQVKLKERLT 278
Query: 275 GK--RYLLVMDDVWNVNRAKWISLKRYLMGGAKGSRILITTRTHQVAQTFETILSHHLKE 334
G +LLV+DD+WN N A W L++ + A+GS+IL+TTR+ +VA + H+L+
Sbjct: 279 GTGLPFLLVLDDLWNENFADWDLLRQPFIHAAQGSQILVTTRSQRVASIMCAVHVHNLQP 338
Query: 335 LDEEKSWKLFRKMAFSNESEVLENSKLVVIGKEIVTKLKGSPLAIRVIGSYLYSKKSEKD 394
L + W LF K F N+ L N ++ + + IV K +G PLA++ +G L + +
Sbjct: 339 LSDGDCWSLFMKTVFGNQEPCL-NREIGDLAERIVHKCRGLPLAVKTLGGVLRFEGKVIE 398
Query: 395 WLSFKDHELDTIMQQENEIQSILKISFNHLSSSLKHCFTYCALFSKDYHYEIRKNDLIKQ 454
W + + ++ + +L++S+ +L + LK CF YC++F K + +E K+ ++
Sbjct: 399 WERVLSSRIWDLPADKSNLLPVLRVSYYYLPAHLKRCFAYCSIFPKGHAFE--KDKVVLL 458
Query: 455 WMAQGFI-QPHNKKAMEDVGDDYFEELLGRSFFQDIRKNKWGEIKKFKMHDIIHDLA--- 514
WMA+GF+ Q + K +E++G++YF EL RS Q + ++ MHD I++LA
Sbjct: 459 WMAEGFLQQTRSSKNLEELGNEYFSELESRSLLQKTK-------TRYIMHDFINELAQFA 518
Query: 515 ----CSVVENDCVLANDDTKSIDKRTRHVSISAFNSMTRWKLITKSLIEAKNLRTL---- 574
S E+ C L + +RTR++S N + ++L E K LRT
Sbjct: 519 SGEFSSKFEDGCKL------QVSERTRYLSYLRDNYAEPMEF--EALREVKFLRTFLPLS 578
Query: 575 --NYARRHHID-------LSNHLRLRTLNLEFHFV----PKCIGKMKHLRYINITYCYID 634
N +R +D L RLR L+L + + P + H R+++++ ++
Sbjct: 579 LTNSSRSCCLDQMVSEKLLPTLTRLRVLSLSHYKIARLPPDFFKNISHARFLDLSRTELE 638
Query: 635 FLPKAVTKLYHLETLIIRGCLELRELSSDIKNLINLRHLDIKDFKHVWSYMPKGMGSMTT 694
LPK++ +Y+L+TL++ C L+EL +DI NLINLR+LD+ K MP+ G + +
Sbjct: 639 KLPKSLCYMYNLQTLLLSYCSSLKELPTDISNLINLRYLDLIGTK--LRQMPRRFGRLKS 698
Query: 695 LQTMNLFILGENKGGELSELNGLVNLRGSLSIQQLQFCKPIGLENVKYLEEKSRIQKLEL 754
LQT+ F + + G +SEL GL +L G L I +LQ + L K +++++
Sbjct: 699 LQTLTTFFVSASDGSRISELGGLHDLHGKLKIVELQRVVDVADAAEANLNSKKHLREIDF 758
Query: 755 HWKTYQRESKIDDEDER------VLESLKPHSNLQKIRIEGYRGLKLCNWFSFDSIVNLV 814
W+T S+ + R V E L+PH +++K+ IE Y+G + +W S S +V
Sbjct: 759 VWRTGSSSSENNTNPHRTQNEAEVFEKLRPHRHIEKLAIERYKGRRFPDWLSDPSFSRIV 818
Query: 815 FIKLFNCEKLQQLPRFDRFPFLKHLHLEDLPSIEYIAINNYVSSSMTTF-----FPSLEN 874
I+L C+ LP + P LK LH+ + ++ I Y S F SLE
Sbjct: 819 CIRLRECQYCTSLPSLGQLPCLKELHISGMVGLQSIGRKFYFSDQQLRDQDQQPFRSLET 878
Query: 875 LSIIKLPNLKEW-----WKGESI------------DQNTSFPTILRHLSQLKIHYCRQLA 934
L LP+ +EW +G+ + + PT L L L I+ C L
Sbjct: 879 LRFDNLPDWQEWLDVRVTRGDLFPSLKKLFILRCPELTGTLPTFLPSLISLHIYKCGLLD 938
Query: 935 SIPQHGPLQSLDIRDISLQLFELVIKMTATNIIFLPNDLFSNVTHLQSLVIGRCFNLKMS 971
P H +++ +S IK + ++ P + F+N L L + +C +L S
Sbjct: 939 FQPDHHEYSYRNLQTLS-------IKSSCDTLVKFPLNHFAN---LDKLEVDQCTSL-YS 998
BLAST of Cucsa.328080 vs. TAIR10
Match:
AT3G14460.1 (AT3G14460.1 LRR and NB-ARC domains-containing disease resistance protein)
HSP 1 Score: 354.0 bits (907), Expect = 3.2e-97
Identity = 256/874 (29.29%), Postives = 435/874 (49.77%), Query Frame = 1
Query: 1 MAESILCSLAGSIITKLG-SFALQDLGLLWGFHDELDKLKGTVSALEAVLLDAEEKQSKS 60
MA S L S A ++ ++ S L +L L +LK + VL DA+++
Sbjct: 1 MANSYLSSCANVMVERINTSQELVELCKGKSSSALLKRLKVALVTANPVLADADQRAEHV 60
Query: 61 RAVKDWILKLKDTFYDIDDLLDVFSYESLKRQVMTKHRTNNTKKVRIFFSKSNQIAFRLK 120
R VK W+ +KD F+ +D+LD E+L+R+V+ + + + A + K
Sbjct: 61 REVKHWLTGIKDAFFQAEDILDELQTEALRRRVVAEAGGLGGLFQNLMAGRE---AIQKK 120
Query: 121 MSQKIKRVREKLDAIAMDKTQFNL--YENTREIQDDESTKRLETTSFIREGEIIGRDDDK 180
+ K+++V L+ L Y TRE Q ++++ + +G ++GR +DK
Sbjct: 121 IEPKMEKVVRLLEHHVKHIEVIGLKEYSETREPQWRQASR--SRPDDLPQGRLVGRVEDK 180
Query: 181 KSIIHYLL-DTNIHEDSVAVIAIIGMGGLGKTALVQSIYGDEKVKKHFELTMWVCISEEF 240
++++ LL D I AVI+++GM G+GKT L + ++ D +V +HFE+ MW+ F
Sbjct: 181 LALVNLLLSDDEISIGKPAVISVVGMPGVGKTTLTEIVFNDYRVTEHFEVKMWISAGINF 240
Query: 241 DVKVIIEKIIESLTKKKREPDLQLDTLQSMVREKIDGKRYLLVMDDVWNVNRAKWISLKR 300
+V + + +++ +T + L +LQ +++ + GKR+LLV+DD W+ + ++W S +
Sbjct: 241 NVFTVTKAVLQDITSSAVNTE-DLPSLQIQLKKTLSGKRFLLVLDDFWSESDSEWESFQV 300
Query: 301 YLMGGAKGSRILITTRTHQVAQTFETILSHHLKELDEEKSWKLFRKMAFSNESEVLENSK 360
+GS+I++TTR+ V+ + + +K + E+ W+L + AF N S N +
Sbjct: 301 AFTDAEEGSKIVLTTRSEIVSTVAKAEKIYQMKLMTNEECWELISRFAFGNISVGSINQE 360
Query: 361 LVVIGKEIVTKLKGSPLAIRVIGSYLYSKKSEKDWLSFKDHELDTIMQQENEIQSILKIS 420
L IGK I + KG PLA R I S+L SK + DW + + N I +LK+S
Sbjct: 361 LEGIGKRIAEQCKGLPLAARAIASHLRSKPNPDDWYAVSKN----FSSYTNSILPVLKLS 420
Query: 421 FNHLSSSLKHCFTYCALFSKDYHYEIRKNDLIKQWMAQGFI-QPHNKKAMEDVGDDYFEE 480
++ L LK CF C++F K + ++ + +L+ WMA + QP + + +ED+G+DY +
Sbjct: 421 YDSLPPQLKRCFALCSIFPKGHVFD--REELVLLWMAIDLLYQPRSSRRLEDIGNDYLGD 480
Query: 481 LLGRSFFQDIRKNKWGEIKKFKMHDIIHDLACSVVENDCV-LANDDTKSIDKRTRHVSIS 540
L+ +SFFQ + + F MHD+++DLA +V + C L +D+ I TRH S S
Sbjct: 481 LVAQSFFQRLDIT----MTSFVMHDLMNDLAKAVSGDFCFRLEDDNIPEIPSTTRHFSFS 540
Query: 541 --------AFNSMTRWKLITKSL--IEAKNLRTLNYARRHHIDLSNHLR-LRTLNLEFHF 600
AF S+ + + L +L +L + L N L LR L+L +
Sbjct: 541 RSQCDASVAFRSICGAEFLRTILPFNSPTSLESLQLTEKVLNPLLNALSGLRILSLSHYQ 600
Query: 601 V---PKCIGKMKHLRYINITYCYIDFLPKAVTKLYHLETLIIRGCLELRELSSDIKNLIN 660
+ PK + +K LRY++++ I LP+ V L +L+TL++ C +L L I LIN
Sbjct: 601 ITNLPKSLKGLKLLRYLDLSSTKIKELPEFVCTLCNLQTLLLSNCRDLTSLPKSIAELIN 660
Query: 661 LRHLDIKDFKHVWSYMPKGMGSMTTLQTMNLFILGENKGGELSELNGLVNLRGSLSIQQL 720
LR LD+ V MP G+ + +LQ ++ F++G G L EL L +LRG+L I +L
Sbjct: 661 LRLLDLVGTPLV--EMPPGIKKLRSLQKLSNFVIGRLSGAGLHELKELSHLRGTLRISEL 720
Query: 721 QFCKPIGLENVKYLEEKSRIQKLELHWKTYQRE------SKIDDEDERVLESLKPHSNLQ 780
Q L+ K + L L W + + + + VL L+PH +L+
Sbjct: 721 QNVAFASEAKDAGLKRKPFLDGLILKWTVKGSGFVPGSFNALACDQKEVLRMLEPHPHLK 780
Query: 781 KIRIEGYRGLKLCNWFSFDSIVNLVFIKLFNCEKLQQLPRFDRFPFLKHLHLEDLPSIEY 840
IE Y+G W S + + L +C LP + P LK+L +E ++
Sbjct: 781 TFCIESYQGGAFPKWLGDSSFFGITSVTLSSCNLCISLPPVGQLPSLKYLSIEKFNILQK 840
Query: 841 IAINNYV--SSSMTTFFPSLENLSIIKLPNLKEW 847
+ ++ + ++S F SL+ L +P EW
Sbjct: 841 VGLDFFFGENNSRGVPFQSLQILKFYGMPRWDEW 856
HSP 2 Score: 56.2 bits (134), Expect = 1.4e-07
Identity = 67/272 (24.63%), Postives = 111/272 (40.81%), Query Frame = 1
Query: 773 DSIVNLVFIKLFNCEKLQQLPRFDRFPFLKHLHLEDLPSIEYIAINNYVSSSMTTFFPSL 832
D ++ ++K+ + L +LP+ L+ LH++ + + ++T +P+L
Sbjct: 1070 DDETDMEYLKVTDISHLMELPQN-----LQSLHIDSCDGLTSLP------ENLTESYPNL 1129
Query: 833 ENLSIIKLPNLKEWWKGESIDQNTSFPTILRHLSQLKIHYCRQLASIPQHGPLQSLDIRD 892
L II +L+ + + PT L+ L I C++L P +S
Sbjct: 1130 HELLIIACHSLESF-------PGSHPPTTLK---TLYIRDCKKLNFTESLQPTRSYS--- 1189
Query: 893 ISLQLFELVIKMTATNIIFLPNDLFSNVTHLQSLVIGRC-----FNLKMSFDDDNVRWKE 952
QL L I + +N++ P LF L+SL I C F++ DD +
Sbjct: 1190 ---QLEYLFIGSSCSNLVNFPLSLFPK---LRSLSIRDCESFKTFSIHAGLGDDRI---- 1249
Query: 953 LGSLRTLRLCFIPKLEYLPKGFQYLKALEHLELLWCENLACILGIEHLTSLSRLEISNCP 1012
+L +L + P LE P+G G+ LS + +SNC
Sbjct: 1250 --ALESLEIRDCPNLETFPQG----------------------GLP-TPKLSSMLLSNCK 1282
Query: 1013 NLTSLPEGMTQLISLTCLIIDDCPNLSTLPEG 1040
L +LPE + L SL L I CP + T+P G
Sbjct: 1310 KLQALPEKLFGLTSLLSLFIIKCPEIETIPGG 1282
HSP 3 Score: 42.0 bits (97), Expect = 2.7e-03
Identity = 33/96 (34.38%), Postives = 50/96 (52.08%), Query Frame = 1
Query: 948 LGSLRTLRLCFIPKLEYLPKGFQYLKALEHLELLWCENLACILGIEHLTSLSRLEISNCP 1007
L LR L L ++ LPK + LK L +L+L + + L +L L +SNC
Sbjct: 572 LSGLRILSLSHY-QITNLPKSLKGLKLLRYLDLSSTKIKELPEFVCTLCNLQTLLLSNCR 631
Query: 1008 NLTSLPEGMTQLISLTCLIIDDCPNLSTLPEGLHHL 1044
+LTSLP+ + +LI+L L + P L +P G+ L
Sbjct: 632 DLTSLPKSIAELINLRLLDLVGTP-LVEMPPGIKKL 665
BLAST of Cucsa.328080 vs. TAIR10
Match:
AT3G07040.1 (AT3G07040.1 NB-ARC domain-containing disease resistance protein)
HSP 1 Score: 267.3 bits (682), Expect = 4.0e-71
Identity = 254/946 (26.85%), Postives = 434/946 (45.88%), Query Frame = 1
Query: 27 LLWGFHDELDKLKGTVSALEAVLLDAEEK------QSKSRAVKDWILKLKDTFYDIDDLL 86
LL G H E+DK+K + +++ L D + + ++ + ++ +D Y I+D+L
Sbjct: 23 LLSGVHGEIDKMKKELLIMKSFLEDTHKHGGNGSTTTTTQLFQTFVANTRDLAYQIEDIL 82
Query: 87 DVFSYESLKRQVMTKHRTNNTKKVRIFFSKSNQIAFRLKMSQKIKRVREKLDAIAMDKTQ 146
D F Y H + K+ F + R ++QK+ V + +I+ +
Sbjct: 83 DEFGYHI--------HGYRSCAKIWRAFHFPRYMWARHSIAQKLGMVNVMIQSISDSMKR 142
Query: 147 FNLYENTREIQ----DDESTKRL----ETTSFIREGEIIGRDDDKKSIIHYLLDTNIHED 206
+ EN + DD K + E++ F E ++G D K +I LL E
Sbjct: 143 YYHSENYQAALLPPIDDGDAKWVNNISESSLFFSENSLVGIDAPKGKLIGRLLSP---EP 202
Query: 207 SVAVIAIIGMGGLGKTALVQSIYGDEKVKKHFELTMWVCISEEFDVKVIIEKIIESLTKK 266
V+A++GMGG GKT L +I+ + V++HFE WV IS+ + ++ + +I+ K
Sbjct: 203 QRIVVAVVGMGGSGKTTLSANIFKSQSVRRHFESYAWVTISKSYVIEDVFRTMIKEFYK- 262
Query: 267 KREPDLQLDT---------LQSMVREKIDGKRYLLVMDDVWNVNRAKWISLKRYLMGGAK 326
E D Q+ L + E + KRY++V+DDVW + IS+ L G
Sbjct: 263 --EADTQIPAELYSLGYRELVEKLVEYLQSKRYIVVLDDVWTTGLWREISIA--LPDGIY 322
Query: 327 GSRILITTRTHQVAQTFETILS--HHLKELDEEKSWKLFRKMAFSNESEVLENSKLVVIG 386
GSR+++TTR VA I S H ++ L E+++W LF AF E L I
Sbjct: 323 GSRVMMTTRDMNVASFPYGIGSTKHEIELLKEDEAWVLFSNKAFPASLEQCRTQNLEPIA 382
Query: 387 KEIVTKLKGSPLAIRVIGSYLYSKKSEKDWLSFKDHELDTIMQQENE---IQSILKISFN 446
+++V + +G PLAI +GS + +KK E +W L+ + +E ++SI+ +SFN
Sbjct: 383 RKLVERCQGLPLAIASLGSMMSTKKFESEWKKVYS-TLNWELNNNHELKIVRSIMFLSFN 442
Query: 447 HLSSSLKHCFTYCALFSKDYHYEIRKNDLIKQWMAQGFIQPHNKKAMEDVGDDYFEELLG 506
L LK CF YC+LF +Y +++ LI+ WMAQ F++P E+V D Y EL+
Sbjct: 443 DLPYPLKRCFLYCSLFP--VNYRMKRKRLIRMWMAQRFVEPIRGVKAEEVADSYLNELVY 502
Query: 507 RSFFQDIRKNKWGEIKKFKMHDIIHDLACSV--VENDCVLANDDTKSIDKR-------TR 566
R+ Q I N +G K FKMHD+I ++A SV +E C + NDD+ D +R
Sbjct: 503 RNMLQVILWNPFGRPKAFKMHDVIWEIALSVSKLERFCDVYNDDSDGDDAAETMENYGSR 562
Query: 567 HVSISAFNSMTRWKLITKSLIEAKNLRTLNY--ARRHHIDLSNHLR-LRTLNLE---FHF 626
H+ I K +T I A NL +L + +H ++L L LR L+LE
Sbjct: 563 HLCIQ--------KEMTPDSIRATNLHSLLVCSSAKHKMELLPSLNLLRALDLEDSSISK 622
Query: 627 VPKCIGKMKHLRYINITYCYIDFLPKAVTKLYHLETLIIRGCLELRELSSDIKNLINLRH 686
+P C+ M +L+Y+N++ + LPK KL +LETL N +H
Sbjct: 623 LPDCLVTMFNLKYLNLSKTQVKELPKNFHKLVNLETL-------------------NTKH 682
Query: 687 LDIKDFKHVWSYMPKGMGSMTTLQTMNLFILGENKGGELSELNGLVNLRGSLSIQQLQFC 746
I++ +P GM + L+ + F E G S N ++ R I QL+
Sbjct: 683 SKIEE-------LPLGMWKLKKLRYLITFRRNE---GHDSNWNYVLGTRVVPKIWQLKDL 742
Query: 747 KPIG--------LENVKYLEEKSRIQKLELHWKTYQRE----------------SKIDDE 806
+ + ++N+ + + +RI L + + + R+ + ID+E
Sbjct: 743 QVMDCFNAEDELIKNLGCMTQLTRI-SLVMVRREHGRDLCDSLNKIKRIRFLSLTSIDEE 802
Query: 807 DERVLESLKPHSNLQKIRIEGYRGLKLCNWFSFDSIVNLVFIKLFNCEKLQQLPRFDRFP 866
+ ++ L ++++K+ + G + ++ +W F+++ NL ++ L R +
Sbjct: 803 EPLEIDDLIATASIEKLFLAG-KLERVPSW--FNTLQNLTYLGL----------RGSQLQ 862
Query: 867 FLKHLHLEDLPSIEYIAINNYVSSSMTTFFPSLENLSIIKLPNLKEWWKGESIDQNTSFP 902
L ++ LP + +++ N F +NL I+++ +K + I+ F
Sbjct: 863 ENAILSIQTLPRLVWLSFYNAYMGPRLRFAQGFQNLKILEIVQMKHLTE-VVIEDGAMF- 892
BLAST of Cucsa.328080 vs. TAIR10
Match:
AT3G46730.1 (AT3G46730.1 NB-ARC domain-containing disease resistance protein)
HSP 1 Score: 262.3 bits (669), Expect = 1.3e-69
Identity = 199/664 (29.97%), Postives = 331/664 (49.85%), Query Frame = 1
Query: 8 SLAGSIITKLGSFALQDLGLLWGFHDELDKLKGTVSALEAVLLDAEEKQSKSRAVKDWIL 67
++ G ++ K+G + + ++ L G D+L++LK ++ + L D E ++ + K+W
Sbjct: 4 AVTGFVLNKIGGYLINEVLALMGVKDDLEELKTELTCIHGYLKDVEAREREDEVSKEWTK 63
Query: 68 KLKDTFYDIDDLLDVFSYE----SLKRQVMTKHRTNNTKKVRIFFSKSNQIAFRLKMSQK 127
+ D YDI+D+LD + + SL+R ++ TN K R ++ I +
Sbjct: 64 LVLDIAYDIEDVLDTYFLKLEERSLRRGLL--RLTNKIGKKRDAYNIVEDIRTLKRRILD 123
Query: 128 IKRVREKLDAIAMDKTQFNLYENTREIQDDESTKRLETTSFIREGE-IIGRDDDKKSIIH 187
I R RE + ++ + N R ++L + + E ++G +DD K ++
Sbjct: 124 ITRKRETFGIGSFNEPRGENITNVR-------VRQLRRAPPVDQEELVVGLEDDVKILLV 183
Query: 188 YLLDTNIHEDSVAVIAIIGMGGLGKTALVQSIYGDEKVKKHFELTMWVCISEEFDVKVII 247
LL N +D +I+I GMGGLGKTAL + +Y VK+ F+ W +S+E+ + I+
Sbjct: 184 KLLSDN-EKDKSYIISIFGMGGLGKTALARKLYNSGDVKRRFDCRAWTYVSQEYKTRDIL 243
Query: 248 EKIIESLTKKKREPDLQL------DTLQSMVREKIDGKRYLLVMDDVWNVNRAKWISLKR 307
+II SL E ++ + L+ + ++GK Y++V+DDVW+ + W SLKR
Sbjct: 244 IRIIRSLGIVSAEEMEKIKMFEEDEELEVYLYGLLEGKNYMVVVDDVWDPD--AWESLKR 303
Query: 308 YLMGGAKGSRILITTRTHQVAQTFE-TILSHHLKELDEEKSWKLFRKMAFSNESEVLENS 367
L +GS+++ITTR +A+ E T+ +H L+ L E+SW LF + AFSN +V E+
Sbjct: 304 ALPCDHRGSKVIITTRIRAIAEGVEGTVYAHKLRFLTFEESWTLFERKAFSNIEKVDED- 363
Query: 368 KLVVIGKEIVTKLKGSPLAIRVIGSYLYSKKSEKDWLSFKDHELDTIMQQENEIQSILKI 427
L GKE+V K G PLAI V+ S L S+K +W + I ++ +
Sbjct: 364 -LQRTGKEMVKKCGGLPLAI-VVLSGLLSRKRTNEWHEVCASLWRRLKDNSIHISTVFDL 423
Query: 428 SFNHLSSSLKHCFTYCALFSKDYHYEIRKNDLIKQWMAQGFIQPHNKKAMEDVGDDYFEE 487
SF + LK CF Y ++F +D YEI+ LI +A+GFIQ + MEDV Y +E
Sbjct: 424 SFKEMRHELKLCFLYFSVFPED--YEIKVEKLIHLLVAEGFIQEDEEMMMEDVARCYIDE 483
Query: 488 LLGRSFFQDIRKNKWGEIKKFKMHDIIHDLACSVVE--NDCVLANDDTKSIDKRTRHVSI 547
L+ RS + R + G++ ++HD++ DLA + N + N+ S D R V
Sbjct: 484 LVDRSLVKAERIER-GKVMSCRIHDLLRDLAIKKAKELNFVNVYNEKQHSSDICRREVVH 543
Query: 548 SAFNSM----TRWKLITKSLIEAKNLRTLNYARRHHIDLSNHLRLRTLNLE--------- 607
N R +S + R Y ++ L LR LN+E
Sbjct: 544 HLMNDYYLCDRRVNKRMRSFLFIGERRGFGYVNTTNLKLK---LLRVLNMEGLLFVSKNI 603
Query: 608 FHFVPKCIGKMKHLRYINITYCYIDFLPKAVTKLYHLETLIIRGCLELRELSSDIKNLIN 645
+ +P IG++ HLRY+ I Y+ LP +++ L L+TL G + + ++D+ L +
Sbjct: 604 SNTLPDVIGELIHLRYLGIADTYVSILPASISNLRFLQTLDASG-NDPFQYTTDLSKLTS 645
BLAST of Cucsa.328080 vs. TAIR10
Match:
AT1G58848.1 (AT1G58848.1 Disease resistance protein (CC-NBS-LRR class) family)
HSP 1 Score: 249.6 bits (636), Expect = 8.6e-66
Identity = 277/1086 (25.51%), Postives = 476/1086 (43.83%), Query Frame = 1
Query: 9 LAGSIIT----KLGSFALQDLGLLWGFHDELDKLKGTVSALEAVLLDAEEKQSKSRAVKD 68
+AG +I+ L + Q+ L G D++ +LK ++ L + L DA+ K+ S VK+
Sbjct: 1 MAGELISFGIQNLWNLLSQECELFQGVEDQVTELKRDLNLLSSFLKDADAKKHTSAVVKN 60
Query: 69 WILKLKDTFYDIDDLLDVFSYESLKRQVMTKHRTNNTKKVRIFFSKSNQIAFRLK-MSQK 128
+ ++K+ YD +D ++ F E + T + +++ + A + +S +
Sbjct: 61 CVEEIKEIIYDGEDTIETFVLE--QNLGKTSGIKKSIRRLACIIPDRRRYALGIGGLSNR 120
Query: 129 IKRVREKLDAIAMDKTQFNLYENTREIQDDESTKRLETTSFIREGEIIGRDDDKKSIIHY 188
I +V + + + + + ++ Q D+ + S + + +G + + K ++ Y
Sbjct: 121 ISKVIRDMQSFGVQQAIVD--GGYKQPQGDKQREMRPRFSKDDDSDFVGLEANVKKLVGY 180
Query: 189 LLDTNIHEDSVAVIAIIGMGGLGKTALVQSIYGDEKVKKHFELTMWVCISEEFDVKVIIE 248
L+D E +V V++I GMGGLGKT L + ++ E VK F+ WVC+S++F + +
Sbjct: 181 LVD----EANVQVVSITGMGGLGKTTLAKQVFNHEDVKHQFDGLSWVCVSQDFTRMNVWQ 240
Query: 249 KIIESLTKKKREP---DLQLDTLQSMVREKIDGKRYLLVMDDVWNVNRAKWISLKRYLMG 308
KI+ L K+ E ++ DTLQ + ++ + L+V+DD+W + W L + +
Sbjct: 241 KILRDLKPKEEEKKIMEMTQDTLQGELIRLLETSKSLIVLDDIW--EKEDW-ELIKPIFP 300
Query: 309 GAKGSRILITTRTHQVAQTFETILSHHLKE-LDEEKSWKLFRKMA--FSNESEVLENSKL 368
KG ++L+T+R VA T + E L E SW LF+++A + +E + +
Sbjct: 301 PTKGWKVLLTSRNESVAMRRNTSYINFKPECLTTEDSWTLFQRIALPMKDAAEFKIDEEK 360
Query: 369 VVIGKEIVTKLKGSPLAIRVIGSYLYSKKSEKDWLSFKDHELDTIM--------QQENEI 428
+GK ++ G PLAIRV+G L K + DW ++ ++ N
Sbjct: 361 EELGKLMIKHCGGLPLAIRVLGGMLAEKYTSHDWRRLSENIGSHLVGGRTNFNDDNNNTC 420
Query: 429 QSILKISFNHLSSSLKHCFTYCALFSKDYHYEIRKNDLIKQWMAQGFIQP--HNKKAMED 488
+L +SF L S LKHCF Y A F D YEI +L W A+G QP ++ + + D
Sbjct: 421 NYVLSLSFEELPSYLKHCFLYLAHFPDD--YEINVKNLSYYWAAEGIFQPRHYDGEIIRD 480
Query: 489 VGDDYFEELLGRSFFQDIRKNKWGEIKKFKMHDIIHDLACSVVENDCVLANDDTKSIDKR 548
VGD Y EEL+ R+ R K + +HD++ ++ + + L T S
Sbjct: 481 VGDVYIEELVRRNMVISERDVKTSRFETCHLHDMMREVCLLKAKEENFL--QITSSRTST 540
Query: 549 TRHVSISAFNSMTRWKLITKSL---IEAKNLRTLNYARRHHIDL---------SNHLRLR 608
+SI + IT + I LR+L ++ S+ +RL
Sbjct: 541 GNSLSIVTSRRLVYQYPITLDVEKDINDPKLRSLVVVANTYMFWGGWSWMLLGSSFIRLE 600
Query: 609 TLN-LEFH-------FVPKCIGKMKHLRYINITYCYIDFLPKAVTKLYHLETLIIRGCLE 668
L L+ H + IG++ HLRY+N+ + + +P + L +L+ LI +
Sbjct: 601 LLRVLDIHRAKLKGGKLASSIGQLIHLRYLNLKHAEVTHIPYS---LGNLKLLIYLNLVI 660
Query: 669 LRELSSDIKNLINLRHLDIKDFKHVWSYMPKGMGSMTTLQTMNLFILG-----ENKGGEL 728
L S+ + N++ +++ +++ +PK MG T L+ NL L K L
Sbjct: 661 LVSGSTLVPNVLK----EMQQLRYL--ALPKDMGRKTKLELSNLVKLETLKNFSTKNCSL 720
Query: 729 SELNGLVNLRGSLSIQQLQFCKPIGLE-------NVKYLEEKSRIQKLELHWKTYQRESK 788
+L G+V LR +L+I + K LE +KYLE + I L +T +
Sbjct: 721 EDLRGMVRLR-TLTI---ELRKETSLETLAASIGGLKYLESLT-ITDLGSEMRTKEAGIV 780
Query: 789 IDDEDERVLESLKPHSNLQKIRIEGYRGLKLCNWFSFDSIVNLVFIKLFNCEKLQQLPRF 848
D L+ + ++ Y +L F S + ++++ E+
Sbjct: 781 FD------------FVYLKTLTLKLYMP-RLSKEQHFPSHLTTLYLQHCRLEE------- 840
Query: 849 DRFPFLKHLHLEDLPSIEYIAINNYVSSSMTTFFPSLENLSIIKLPNLKEW--WKGESID 908
D P L+ LH + + + + FP L+ LSI L+EW WK E
Sbjct: 841 DPMPILEKLHQLKELELRRKSFSGKEMVCSSGGFPQLQKLSI---KGLEEWEDWKVE--- 900
Query: 909 QNTSFPTILRHLSQLKIHYCRQLASIP-QHGP--LQSLD-----IRDISLQLFELVIKMT 968
+S P L L I CR+L +P +H P L S+ + + + E ++ +
Sbjct: 901 -ESSMPV----LHTLDIRDCRKLKQLPDEHLPSHLTSISLFFCCLEEDPMPTLERLVHLK 960
Query: 969 ATNIIFLPNDLFSNVTHLQSLVIGRCFNLKMSFDDDNVRW----KELGSLRTLRLCFIPK 1028
++F + + LK+S D W + L TL + PK
Sbjct: 961 ELQLLF--RSFSGRIMVCAGSGFPQLHKLKLSELDGLEEWIVEDGSMPQLHTLEIRRCPK 1020
BLAST of Cucsa.328080 vs. NCBI nr
Match:
gi|778722268|ref|XP_011658447.1| (PREDICTED: disease resistance protein RGA2-like [Cucumis sativus])
HSP 1 Score: 1573.1 bits (4072), Expect = 0.0e+00
Identity = 843/1086 (77.62%), Postives = 886/1086 (81.58%), Query Frame = 1
Query: 1 MAESILCSLAGSIITKLGSFALQDLGLLWGFHDELDKLKGTVSALEAVLLDAEEKQSKSR 60
MAESILCSLAGSIITKLGSFALQDLGLLWGFHDELDKLKGTVSALEAVLLDAEEKQSKSR
Sbjct: 1 MAESILCSLAGSIITKLGSFALQDLGLLWGFHDELDKLKGTVSALEAVLLDAEEKQSKSR 60
Query: 61 AVKDWILKLKDTFYDIDDLLDVFSYESLKRQVMTKHRTNNTKKVRIFFSKSNQIAFRLKM 120
AVKDWILKLKDTFYDIDDLLDVFSYESLKRQVMTKHRTNNTKKVRIFFSKSNQIAFRLKM
Sbjct: 61 AVKDWILKLKDTFYDIDDLLDVFSYESLKRQVMTKHRTNNTKKVRIFFSKSNQIAFRLKM 120
Query: 121 SQKIKRVREKLDAIAMDKTQFNLYENTREIQDDESTKRLETTSFIREGEIIGRDDDKKSI 180
SQKIKRVREKLDAIAMDKTQFNLYENTREIQDDESTKRLETTSFIREGEIIGRDDDKKSI
Sbjct: 121 SQKIKRVREKLDAIAMDKTQFNLYENTREIQDDESTKRLETTSFIREGEIIGRDDDKKSI 180
Query: 181 IHYLLDTNIHEDSVAVIAIIGMGGLGKTALVQSIYGDEKVKKHFELTMWVCISEEFDVKV 240
IHYLLDTNIHEDSVAVIAIIGMGGLGKTALVQSIYGDEKVKKHFELTMWVCISEEFDVKV
Sbjct: 181 IHYLLDTNIHEDSVAVIAIIGMGGLGKTALVQSIYGDEKVKKHFELTMWVCISEEFDVKV 240
Query: 241 IIEKIIESLTKKKREPDLQLDTLQSMVREKIDGKRYLLVMDDVWNVNRAKWISLKRYLMG 300
IIEKIIESLTKKKREPDLQLDTLQSMVREKIDGKRYLLVMDDVWNVNRAKWISLKR M
Sbjct: 241 IIEKIIESLTKKKREPDLQLDTLQSMVREKIDGKRYLLVMDDVWNVNRAKWISLKR-KMA 300
Query: 301 GAKGSRILITTRTHQVAQTFET----------ILSHHLKELDEEKSWKLFRKMAFSNESE 360
+ S +L ++ + + T ++ +L EK W F+ +E +
Sbjct: 301 FSNESEVLENSKLVVIGKEIVTKLKGSPLAIRVIGSYLYSKKSEKDWLSFK----DHELD 360
Query: 361 VLENSKLVVIGKEIVTKLKGSPLAIRVIGSYLYSKKSEKDW-LSFKDHELDTIMQQENEI 420
+ + + + + K+ + L+ + + Y KD+ + ++L + I
Sbjct: 361 TIMQQENEI---QSILKISFNHLSSSLKHCFTYCALFSKDYHHEIRKNDLIKQWMAQGFI 420
Query: 421 QSILKISFNHLSSSLKHCFTYCALFSKDYHYEIRKNDLIKQWMAQGFIQPHNKKAMEDVG 480
Q K + + + L + + +IRKN +W G I NK M D+
Sbjct: 421 QPHNKKAMEDVGDDY-----FEELLGRSFFQDIRKN----KW---GEI---NKFKMHDII 480
Query: 481 DDYFEELLGRSFFQDIRKNKWGEIKKFKMHDIIHDLACSVVENDCVLANDDTKSIDKRTR 540
D L S ++ C + +D + T+ +
Sbjct: 481 HD-----LACSVVEN---------------------DCVLANDDTKSIDKRTRHV----- 540
Query: 541 HVSISAFNSMTRWKLITKSLIEAKNLRTLNYARRHHIDLSNHLRLRTLNLEFHFVPKCIG 600
SISAFNSMTRWKLITKSLIEAKNLRTLNYARRHHIDLSNHLRLRTLNLEFHFVPKCIG
Sbjct: 541 --SISAFNSMTRWKLITKSLIEAKNLRTLNYARRHHIDLSNHLRLRTLNLEFHFVPKCIG 600
Query: 601 KMKHLRYINITYCYIDFLPKAVTKLYHLETLIIRGCLELRELSSDIKNLINLRHLDIKDF 660
KMKHLRYINITYCYIDFLPKAVTKLYHLETLIIRGCLELRELSSDIKNLINLRHLDIKDF
Sbjct: 601 KMKHLRYINITYCYIDFLPKAVTKLYHLETLIIRGCLELRELSSDIKNLINLRHLDIKDF 660
Query: 661 KHVWSYMPKGMGSMTTLQTMNLFILGENKGGELSELNGLVNLRGSLSIQQLQFCKPIGLE 720
KHVWSYMPKGMGSMTTLQTMNLFILGENKGGELSELNGLVNLRGSLSIQQLQFCKPIGLE
Sbjct: 661 KHVWSYMPKGMGSMTTLQTMNLFILGENKGGELSELNGLVNLRGSLSIQQLQFCKPIGLE 720
Query: 721 NVKYLEEKSRIQKLELHWKTYQRESKIDDEDERVLESLKPHSNLQKIRIEGYRGLKLCNW 780
NVKYLEEKSRIQKLELHWKTYQRESKIDDEDERVLESLKPHSNLQKIRIEGYRGLKLCNW
Sbjct: 721 NVKYLEEKSRIQKLELHWKTYQRESKIDDEDERVLESLKPHSNLQKIRIEGYRGLKLCNW 780
Query: 781 FSFDSIVNLVFIKLFNCEKLQQLPRFDRFPFLKHLHLEDLPSIEYIAINNYVSSSMTTFF 840
FSFDSIVNLVFIKLFNCEKLQQLPRFDRFPFLKHLHLEDLPSIEYIAINNYVSSSMTTFF
Sbjct: 781 FSFDSIVNLVFIKLFNCEKLQQLPRFDRFPFLKHLHLEDLPSIEYIAINNYVSSSMTTFF 840
Query: 841 PSLENLSIIKLPNLKEWWKGESIDQNTSFPTILRHLSQLKIHYCRQLASIPQHGPLQSLD 900
PSLENLSIIKLPNLKEWWKGESIDQNTSFPTILRHLSQLKIHYCRQLASIPQHGPLQSLD
Sbjct: 841 PSLENLSIIKLPNLKEWWKGESIDQNTSFPTILRHLSQLKIHYCRQLASIPQHGPLQSLD 900
Query: 901 IRDISLQLFELVIKMTATNIIF------LPNDLFSN----------------------VT 960
IRDISLQLFELVIKMTATNII ND+F VT
Sbjct: 901 IRDISLQLFELVIKMTATNIIVGEDSSSSANDMFIRSSSSLKIWKIDWEFLPNDLFSNVT 960
Query: 961 HLQSLVIGRCFNLKMSFDDDNVRWKELGSLRTLRLCFIPKLEYLPKGFQYLKALEHLELL 1020
HLQSLVIGRCFNLKMSFDDDNVRWKELGSLRTLRLCFIPKLEYLPKGFQYLKALEHLELL
Sbjct: 961 HLQSLVIGRCFNLKMSFDDDNVRWKELGSLRTLRLCFIPKLEYLPKGFQYLKALEHLELL 1020
Query: 1021 WCENLACILGIEHLTSLSRLEISNCPNLTSLPEGMTQLISLTCLIIDDCPNLSTLPEGLH 1048
WCENLACILGIEHLTSLSRLEISNCPNLTSLPEGMTQLISLTCLIIDDCPNLSTLPEGLH
Sbjct: 1021 WCENLACILGIEHLTSLSRLEISNCPNLTSLPEGMTQLISLTCLIIDDCPNLSTLPEGLH 1030
BLAST of Cucsa.328080 vs. NCBI nr
Match:
gi|778722268|ref|XP_011658447.1| (PREDICTED: disease resistance protein RGA2-like [Cucumis sativus])
HSP 1 Score: 75.5 bits (184), Expect = 6.3e-10
Identity = 39/58 (67.24%), Postives = 48/58 (82.76%), Query Frame = 1
Query: 8 SLAGSIITKLGSFALQDLGLLW-GFHDELDKLKGTVSALEAVLLDAEEKQSKSRAVKD 65
SL+ +I TKLGSF+L +LGLLW GFH+ELDKLK T+ A++ VLLDAE KQ KS AVK+
Sbjct: 1031 SLSANIATKLGSFSLLELGLLWIGFHEELDKLKDTLFAIQVVLLDAEHKQYKSYAVKE 1088
HSP 2 Score: 1525.0 bits (3947), Expect = 0.0e+00
Identity = 790/1085 (72.81%), Postives = 895/1085 (82.49%), Query Frame = 1
Query: 1 MAESILCSLAGSIITKLGSFALQDLGLLW-GFHDELDKLKGTVSALEAVLLDAEEKQSKS 60
MAESIL +LA +I TKLGSF L +LGLLW GFH+ELDKLK T+SA++AVLLDAEEKQ KS
Sbjct: 1 MAESILFTLAANIATKLGSFPLHELGLLWTGFHEELDKLKDTLSAIQAVLLDAEEKQYKS 60
Query: 61 RAVKDWILKLKDTFYDIDDLLDVFSYESLKRQVMTKHRTNNTKKVRIFFSKSNQIAFRLK 120
AVK+W+ +LKD FYDIDDL+D FSYES+KRQVM KHRTNN K+VRIFFS+SNQIAFRLK
Sbjct: 61 YAVKEWVSRLKDAFYDIDDLMDEFSYESIKRQVMIKHRTNN-KQVRIFFSESNQIAFRLK 120
Query: 121 MSQKIKRVREKLDAIAMDKTQFNLYENTREIQDDESTKRLETTSFIREGEIIGRDDDKKS 180
M KIKRVREKLD +A+DK QFNL E TREI++DE+TKR ET+SFI EGE+IGRDDDKK
Sbjct: 121 MGYKIKRVREKLDIVAIDKAQFNLSEYTREIRNDETTKRPETSSFILEGEVIGRDDDKKG 180
Query: 181 IIHYLLDTNIHEDSVAVIAIIGMGGLGKTALVQSIYGDEKVKKHFELTMWVCISEEFDVK 240
I+H+LLDTN+ +++VAV+AIIGMGGLGKTAL QSIYGD K KHFELTMWVCISEEFDVK
Sbjct: 181 IVHFLLDTNVAQENVAVVAIIGMGGLGKTALAQSIYGDMKENKHFELTMWVCISEEFDVK 240
Query: 241 VIIEKIIESLTKKKREPDLQLDTLQSMVREKIDGKRYLLVMDDVWNVNRAKWISLKRYLM 300
+I+EKIIESLTKK+ EP+LQLDTLQ+M+REKIDGKRYLLVMDDVWNVNR KWI+LK +L
Sbjct: 241 IIVEKIIESLTKKRPEPNLQLDTLQNMLREKIDGKRYLLVMDDVWNVNREKWINLKAFLT 300
Query: 301 GGAKGSRILITTRTHQVAQTFETILSHHLKELDEEKSWKLFRKMAFSNESEVLENSKLVV 360
GGAKGSRILITTRTHQVA T ET+L HHL EL++ SW+LFRKMAFSNESEVLENSKLV+
Sbjct: 301 GGAKGSRILITTRTHQVAHTSETVLFHHLSELNKNSSWELFRKMAFSNESEVLENSKLVI 360
Query: 361 IGKEIVTKLKGSPLAIRVIGSYLYSKKSEKDWLSFKDHELDTIMQQENEIQSILKISFNH 420
IGKEIV LKGSPLA+RVIGSYLYSKK+EKDWLSFKD+EL TIMQQENEIQSILKISFN
Sbjct: 361 IGKEIVANLKGSPLALRVIGSYLYSKKTEKDWLSFKDNELGTIMQQENEIQSILKISFNQ 420
Query: 421 LSSSLKHCFTYCALFSKDYHYEIRKNDLIKQWMAQGFIQPHNKKAMEDVGDDYFEELLGR 480
LSS LK CFTYCALF KD YEI+K+DLIKQWMAQGFIQP NKK MEDVGDDYF+ELLGR
Sbjct: 421 LSSGLKQCFTYCALFPKD--YEIQKDDLIKQWMAQGFIQPQNKKTMEDVGDDYFKELLGR 480
Query: 481 SFFQDIRKNKWGEIKKFKMHDIIHDLACSVVENDCVLANDDTKSIDKRTRHVSISAFNSM 540
SFFQDI+KNKWGEIK+FK+HD +HDLACSVVEN CVL NDDTK+IDKRTRHVSIS F S
Sbjct: 481 SFFQDIKKNKWGEIKEFKIHDFMHDLACSVVENVCVLTNDDTKTIDKRTRHVSISTFISK 540
Query: 541 TRWKLITKSLIEAKNLRTLNYARRHHIDLSNHLRLRTLNLEF-HFVPKCIGKMKHLRYIN 600
TRWK+IT+SL EAKNLRTLNYA IDLSNHL+LRTLN++F + VPKCIGKMKHLRY+N
Sbjct: 541 TRWKVITESLKEAKNLRTLNYA-CDKIDLSNHLQLRTLNVDFLYHVPKCIGKMKHLRYVN 600
Query: 601 ITYCYIDFLPKAVTKLYHLETLIIRGCLELRELSSDIKNLINLRHLDIKDF-KHVWSYMP 660
++ +IDFLPK +TKLY+LETLI+R C L L SDIKNLINLRHLDI + WS MP
Sbjct: 601 LSNTWIDFLPKFITKLYNLETLILRNCQRLMGLPSDIKNLINLRHLDIMHCNSNCWSSMP 660
Query: 661 KGMGSMTTLQTMNLFILGENKGGELSELNGLVNLRGSLSIQQLQFCKPIGLENVKYLEEK 720
G+GSMT+LQTMNLF+LG GGELSELNGL +LRGSL I+QLQFC LEN KYLEEK
Sbjct: 661 MGLGSMTSLQTMNLFVLGAKWGGELSELNGLNSLRGSLRIKQLQFCITPNLENAKYLEEK 720
Query: 721 SRIQKLELHWKTYQ--RESKIDDEDERVLESLKPHSNLQKIRIEGYRGLKLCNWFSFDSI 780
S IQKLELHW + + +DEDE+VLE LKPH NLQKIRIEGY+G+KLCNWFSF SI
Sbjct: 721 SGIQKLELHWDSSYGIGRNTFNDEDEKVLECLKPHPNLQKIRIEGYKGVKLCNWFSFGSI 780
Query: 781 VNLVFIKLFNCEKLQQLPRFDRFPFLKHLHLEDLPSIEYIAINNYVSSSMTTFFPSLENL 840
+LV IKL CEKL+ LP+FD+FPFLKHLHLEDLP+IE+I NNYVSSS+TTFFPSLE L
Sbjct: 781 GSLVTIKLCECEKLKHLPQFDQFPFLKHLHLEDLPNIEFIDDNNYVSSSLTTFFPSLEKL 840
Query: 841 SIIKLPNLKEWWKGESIDQNTSFPTILRHLSQLKIHYCRQLASIPQHGPLQSLDIRDISL 900
SII+LP LKEWWKGE IDQ T FPT L HLS+LKI++C QL SIPQHGPL+SLD+ +SL
Sbjct: 841 SIIQLPKLKEWWKGEFIDQTTPFPTTLHHLSRLKINHCPQLGSIPQHGPLRSLDVSGVSL 900
Query: 901 QLFELVIKMTATNII------------------------FLP-NDLFSNVTHLQSLVIGR 960
QLFELV++M TNII FL +DLFSN+THL+SLVIG
Sbjct: 901 QLFELVMEMATTNIIVGQDSSSSTTRSLSSLSIWDMDFEFLQLHDLFSNMTHLKSLVIGN 960
Query: 961 CFNLKMSFDDDNVRWKELGSLRTLRLCFIPKLEYLPKGFQYLKALEHLELLWCENLACIL 1020
C N+KMS D V WKELGSLR L LC IP+LE LPKG QY+ LE L+L C NL I
Sbjct: 961 CKNIKMSSSRDGVIWKELGSLRRLDLCSIPELECLPKGLQYVTTLECLKLYDCRNLVSIE 1020
Query: 1021 GIEHLTSLSRLEISNCPNLTSLPEGMTQLISLTCLIIDDCPNLSTLPEGLHHL------- 1049
GIEHLTSLS LEI CPNL S P+ M QL SL+ L I+ CPNL +LPEGL H+
Sbjct: 1021 GIEHLTSLSLLEIEYCPNLISYPQEMGQLTSLSRLRINGCPNLPSLPEGLRHVTFMYYED 1080
BLAST of Cucsa.328080 vs. NCBI nr
Match:
gi|700192477|gb|KGN47681.1| (hypothetical protein Csa_6G375730 [Cucumis sativus])
HSP 1 Score: 1401.0 bits (3625), Expect = 0.0e+00
Identity = 701/775 (90.45%), Postives = 714/775 (92.13%), Query Frame = 1
Query: 324 LSHHLKELDEEKSWKLFRKMAFSNESEVL--ENSKLVVIG------------KEIVTKLK 383
L + +E+ +++S K +F E E++ ++ K +I EIVTKLK
Sbjct: 143 LYENTREIQDDESTKRLETTSFIREGEIIGRDDDKKSIIHYLLDTNIHEDSVAEIVTKLK 202
Query: 384 GSPLAIRVIGSYLYSKKSEKDWLSFKDHELDTIMQQENEIQSILKISFNHLSSSLKHCFT 443
GSPLAIRVIGSYLYSKKSEKDWLSFKDHELDTIMQQENEIQSILKISFNHLSSSLKHCFT
Sbjct: 203 GSPLAIRVIGSYLYSKKSEKDWLSFKDHELDTIMQQENEIQSILKISFNHLSSSLKHCFT 262
Query: 444 YCALFSKDYHYEIRKNDLIKQWMAQGFIQPHNKKAMEDVGDDYFEELLGRSFFQDIRKNK 503
YCALFSKDYH+EIRKNDLIKQWMAQGFIQPHNKKAMEDVGDDYFEELLGRSFFQDIRKNK
Sbjct: 263 YCALFSKDYHHEIRKNDLIKQWMAQGFIQPHNKKAMEDVGDDYFEELLGRSFFQDIRKNK 322
Query: 504 WGEIKKFKMHDIIHDLACSVVENDCVLANDDTKSIDKRTRHVSISAFNSMTRWKLITKSL 563
WGEI KFKMHDIIHDLACSVVENDCVLANDDTKSIDKRTRHVSISAFNSMTRWKLITKSL
Sbjct: 323 WGEINKFKMHDIIHDLACSVVENDCVLANDDTKSIDKRTRHVSISAFNSMTRWKLITKSL 382
Query: 564 IEAKNLRTLNYARRHHIDLSNHLRLRTLNLEFHFVPKCIGKMKHLRYINITYCYIDFLPK 623
IEAKNLRTLNYARRHHIDLSNHLRLRTLNLEFHFVPKCIGKMKHLRYINITYCYIDFLPK
Sbjct: 383 IEAKNLRTLNYARRHHIDLSNHLRLRTLNLEFHFVPKCIGKMKHLRYINITYCYIDFLPK 442
Query: 624 AVTKLYHLETLIIRGCLELRELSSDIKNLINLRHLDIKDFKHVWSYMPKGMGSMTTLQTM 683
AVTKLYHLETLIIRGCLELRELSSDIKNLINLRHLDIKDFKHVWSYMPKGMGSMTTLQTM
Sbjct: 443 AVTKLYHLETLIIRGCLELRELSSDIKNLINLRHLDIKDFKHVWSYMPKGMGSMTTLQTM 502
Query: 684 NLFILGENKGGELSELNGLVNLRGSLSIQQLQFCKPIGLENVKYLEEKSRIQKLELHWKT 743
NLFILGENKGGELSELNGLVNLRGSLSIQQLQFCKPIGLENVKYLEEKSRIQKLELHWKT
Sbjct: 503 NLFILGENKGGELSELNGLVNLRGSLSIQQLQFCKPIGLENVKYLEEKSRIQKLELHWKT 562
Query: 744 YQRESKIDDEDERVLESLKPHSNLQKIRIEGYRGLKLCNWFSFDSIVNLVFIKLFNCEKL 803
YQRESKIDDEDERVLESLKPHSNLQKIRIEGYRGLKLCNWFSFDSIVNLVFIKLFNCEKL
Sbjct: 563 YQRESKIDDEDERVLESLKPHSNLQKIRIEGYRGLKLCNWFSFDSIVNLVFIKLFNCEKL 622
Query: 804 QQLPRFDRFPFLKHLHLEDLPSIEYIAINNYVSSSMTTFFPSLENLSIIKLPNLKEWWKG 863
QQLPRFDRFPFLKHLHLEDLPSIEYIAINNYVSSSMTTFFPSLENLSIIKLPNLKEWWKG
Sbjct: 623 QQLPRFDRFPFLKHLHLEDLPSIEYIAINNYVSSSMTTFFPSLENLSIIKLPNLKEWWKG 682
Query: 864 ESIDQNTSFPTILRHLSQLKIHYCRQLASIPQHGPLQSLDIRDISLQLFELVIKMTATNI 923
ESIDQNTSFPTILRHLSQLKIHYCRQLASIPQHGPLQSLDIRDISLQLFELVIKMTATNI
Sbjct: 683 ESIDQNTSFPTILRHLSQLKIHYCRQLASIPQHGPLQSLDIRDISLQLFELVIKMTATNI 742
Query: 924 I----------------------------FLPNDLFSNVTHLQSLVIGRCFNLKMSFDDD 983
I FLPNDLFSNVTHLQSLVIGRCFNLKMSFDDD
Sbjct: 743 IVGEDSSSSANDMFIRSSSSLKIWKIDWEFLPNDLFSNVTHLQSLVIGRCFNLKMSFDDD 802
Query: 984 NVRWKELGSLRTLRLCFIPKLEYLPKGFQYLKALEHLELLWCENLACILGIEHLTSLSRL 1043
NVRWKELGSLRTLRLCFIPKLEYLPKGFQYLKALEHLELLWCENLACILGIEHLTSLSRL
Sbjct: 803 NVRWKELGSLRTLRLCFIPKLEYLPKGFQYLKALEHLELLWCENLACILGIEHLTSLSRL 862
Query: 1044 EISNCPNLTSLPEGMTQLISLTCLIIDDCPNLSTLPEGLHHLLNTPRYAPLIFSH 1057
EISNCPNLTSLPEGMTQLISLTCLIIDDCPNLSTLPEGLHHLLNTPRYAPLIFSH
Sbjct: 863 EISNCPNLTSLPEGMTQLISLTCLIIDDCPNLSTLPEGLHHLLNTPRYAPLIFSH 917
BLAST of Cucsa.328080 vs. NCBI nr
Match:
gi|700192477|gb|KGN47681.1| (hypothetical protein Csa_6G375730 [Cucumis sativus])
HSP 1 Score: 383.3 bits (983), Expect = 1.4e-102
Identity = 207/252 (82.14%), Postives = 216/252 (85.71%), Query Frame = 1
Query: 1 MAESILCSLAGSIITKLGSFALQDLGLLWGFHDELDKLKGTVSALEAVLLDAEEKQSKSR 60
MAESILCSLAGSIITKLGSFALQDLGLLWGFHDELDKLKGTVSALEAVLLDAEEKQSKSR
Sbjct: 1 MAESILCSLAGSIITKLGSFALQDLGLLWGFHDELDKLKGTVSALEAVLLDAEEKQSKSR 60
Query: 61 AVKDWILKLKDTFYDIDDLLDVFSYESLKRQVMTKHRTNNTKKVRIFFSKSNQIAFRLKM 120
AVKDWILKLKDTFYDIDDLLDVFSYESLKRQVMTKHRTNNTKKVRIFFSKSNQIAFRLKM
Sbjct: 61 AVKDWILKLKDTFYDIDDLLDVFSYESLKRQVMTKHRTNNTKKVRIFFSKSNQIAFRLKM 120
Query: 121 SQKIKRVREKLDAIAMDKTQFNLYENTREIQDDESTKRLETTSFIREGEIIGRDDDKKSI 180
SQKIKRVREKLDAIAMDKTQFNLYENTREIQDDESTKRLETTSFIREGEIIGRDDDKKSI
Sbjct: 121 SQKIKRVREKLDAIAMDKTQFNLYENTREIQDDESTKRLETTSFIREGEIIGRDDDKKSI 180
Query: 181 IHYLLDTNIHEDSVAVIAIIGMGGLGKTALVQSIYGDEKVKKHFELTMWVCISE-EFDVK 240
IHYLLDTNIHEDSVA I G ++ S +K +K W+ + E D
Sbjct: 181 IHYLLDTNIHEDSVAEIVTKLKGSPLAIRVIGSYLYSKKSEKD-----WLSFKDHELDTI 240
Query: 241 VIIEKIIESLTK 252
+ E I+S+ K
Sbjct: 241 MQQENEIQSILK 247
HSP 2 Score: 1393.3 bits (3605), Expect = 0.0e+00
Identity = 735/1060 (69.34%), Postives = 833/1060 (78.58%), Query Frame = 1
Query: 1 MAESILCSLAGSIITKLGSFALQDLGLLW-GFHDELDKLKGTVSALEAVLLDAEEKQSKS 60
MAESIL SLA +I TKLGS ALQDLGLLW G H+E+DKL+ T+SA++AVL DAE+KQ KS
Sbjct: 1 MAESILFSLAANIATKLGSLALQDLGLLWTGIHEEIDKLRDTLSAIQAVLHDAEQKQYKS 60
Query: 61 RAVKDWILKLKDTFYDIDDLLDVFSYESLKRQVMTKHRTNN-TKKVRIFFSKSNQIAFRL 120
AVK+W+ +LKD FYD+DDL+D FSYES +RQVMTKHRTNN TK+V IFFSKSNQI FRL
Sbjct: 61 SAVKEWVSRLKDAFYDMDDLMDEFSYESFQRQVMTKHRTNNCTKQVCIFFSKSNQIRFRL 120
Query: 121 KMSQKIKRVREKLDAIAMDKTQFNLYENTREIQDDESTKRLETTSFIREGEIIGRDDDKK 180
KM KIK++REKLD I DKTQFNL++NTREI++DE T+R ET SFI EGE+IGRDDDKK
Sbjct: 121 KMVHKIKKIREKLDTIDKDKTQFNLFDNTREIRNDEMTQRSETCSFILEGEVIGRDDDKK 180
Query: 181 SIIHYLLDTNI-HEDSVAVIAIIGMGGLGKTALVQSIYGDEKVKKHFELTMWVCISEEFD 240
I+H+LLDTNI ++++ V+AIIGMGGLGKTAL QSIYG K KHFELTMWVCISEEFD
Sbjct: 181 CIVHFLLDTNIIAKENIVVVAIIGMGGLGKTALAQSIYGHMKENKHFELTMWVCISEEFD 240
Query: 241 VKVIIEKIIESLTKKKREPDLQLDTLQSMVREKIDGKRYLLVMDDVWNVNRAKWISLKRY 300
VKVI+EKIIESLTKK+ EP+L LDTLQSM+REKIDGK+YLLVMDDVWN R KWI+LK++
Sbjct: 241 VKVIVEKIIESLTKKRPEPNLTLDTLQSMLREKIDGKKYLLVMDDVWNDERTKWINLKKF 300
Query: 301 LMGGAKGSRILITTRTHQVAQTFETILSHHLKELDEEKSWKLFRKMAFSNESEVLENSKL 360
LMGGAKGSRILITTRTHQVA F+T L H L ELD++ SW+LFRKMAFSNESE+LENSKL
Sbjct: 301 LMGGAKGSRILITTRTHQVAHIFDTDLFHDLSELDKDNSWELFRKMAFSNESEMLENSKL 360
Query: 361 VVIGKEIVTKLKGSPLAIRVIGSYLYSKKSEKDWLSFKDHELDTIMQQENEIQSILKISF 420
V IGKEIV KLKGSPLAIRVIGSYLYSKKSEKDWLSFK++ELDTIMQQ+NEIQSILKISF
Sbjct: 361 VGIGKEIVAKLKGSPLAIRVIGSYLYSKKSEKDWLSFKENELDTIMQQKNEIQSILKISF 420
Query: 421 NHLSSSLKHCFTYCALFSKDYHYEIRKNDLIKQWMAQGFIQPHNKKAMEDVGDDYFEELL 480
NHLSSSLK CFTYCALF KD+ EI K+DLIKQWM +GFIQPHNKKAMEDVGD+YF+ELL
Sbjct: 421 NHLSSSLKQCFTYCALFLKDF--EIDKDDLIKQWMGEGFIQPHNKKAMEDVGDEYFKELL 480
Query: 481 GRSFFQDIRKNKWGEIKKFKMHDIIHDLACSVVENDCVLANDDTKSIDKRTRHVSISAFN 540
GRSFFQDI KN+ GEI KFKMHD +HDLAC V END V A DDTK IDKRTRH+SIS F
Sbjct: 481 GRSFFQDISKNQLGEIMKFKMHDFMHDLACFVGENDYVFATDDTKFIDKRTRHLSISPFI 540
Query: 541 SMTRWKLITKSLIEAKNLRTLNYARRHH------IDLSNHLRLRTLNLEFH-FVPKCIGK 600
S TRW++I +SLI AKNLRTLNYA ++ ID SNHLRLRTLNL F VPKCIGK
Sbjct: 541 SKTRWEVIKESLIAAKNLRTLNYACHNYDGDEIEIDFSNHLRLRTLNLIFSTHVPKCIGK 600
Query: 601 MKHLRYINITYCYIDFLPKAVTKLYHLETLIIRGCLELRELSSDIKNLINLRHLDIKDFK 660
MKHLRYIN T CY DFLPK VTKLYHLETLI R C +LREL SDI NLINLRHL I
Sbjct: 601 MKHLRYINFTRCYFDFLPKVVTKLYHLETLIFRECFKLRELPSDITNLINLRHLGINSLI 660
Query: 661 HVWSYMPKGMGSMTTLQTMNLFILGENKGGELSELNGLVNLRGSLSIQQLQFCKPIGLEN 720
SYMPKGMGSMTTLQT+NLFILGEN+GGELSELNGL+NLRGSLSIQQLQFCKPIG+E+
Sbjct: 661 EGLSYMPKGMGSMTTLQTLNLFILGENEGGELSELNGLINLRGSLSIQQLQFCKPIGIES 720
Query: 721 VKYLEEKSRIQKLELHWKTYQRESKIDDEDERVLESLKPHSNLQKIRIEGYRGLKLCNWF 780
K+LEEKS IQKL+L+W +R+ +IDDEDE+VLE LKPH NLQKI I GY G+KLCNWF
Sbjct: 721 AKHLEEKSGIQKLKLYWYHLERKYEIDDEDEKVLECLKPHPNLQKIVINGYGGVKLCNWF 780
Query: 781 SFDSIVNLVFIKLFNCEKLQQLPRFDRFPFLKHLHLEDLPSIEYIAINNYVSSSMTTFFP 840
SFD IVNLV I LFNC KLQQLPRFD+FPFLKHL L+ LP++E+I N+ VSSS+TTFFP
Sbjct: 781 SFDYIVNLVIIDLFNCNKLQQLPRFDQFPFLKHLKLQYLPNVEFIDNNDSVSSSLTTFFP 840
Query: 841 SLENLSIIKLPNLKEWWKGESIDQNTSFPTILRHLSQLKIHYCRQLASIPQHGPLQSLDI 900
SLE L I +LP LKEWWK + IDQ +IPQH L+SL+I
Sbjct: 841 SLEKLRIFRLPKLKEWWKRKLIDQ-----------------------TIPQHRRLESLNI 900
Query: 901 RDISLQLFELVIKMTATNIIFLPNDLFSNVT---------------------------HL 960
+SLQ+FELV++M TNII D S+ T HL
Sbjct: 901 SGVSLQVFELVMEMATTNIIVGSQDSSSSTTSISLSFLSIEDIDFEFLQFHDLFSNMTHL 960
Query: 961 QSLVIGRCFNLKMSFDDDNVRWKELGSLRTLRLCFIPKLEYLPKGFQYLKALEHLELLWC 1020
+SL I C N+KMS D V WK LGSLR L L IP LEYLPK Q + L+ L++ C
Sbjct: 961 KSLWIINCKNIKMSSSLDAVTWKGLGSLRELMLSSIPDLEYLPKSLQCVTTLQSLQIYNC 1020
Query: 1021 ENLACILGIEHL-TSLSRLEISNCPNLTSLPEGMTQLISL 1023
NL I I HL TSLS LEI CPN+T P M+QL SL
Sbjct: 1021 PNLVSIESIRHLTTSLSVLEIHGCPNITFYPHEMSQLASL 1035
BLAST of Cucsa.328080 vs. NCBI nr
Match:
gi|778728520|ref|XP_011659433.1| (PREDICTED: putative disease resistance protein RGA4 [Cucumis sativus])
HSP 1 Score: 1241.1 bits (3210), Expect = 0.0e+00
Identity = 648/1080 (60.00%), Postives = 815/1080 (75.46%), Query Frame = 1
Query: 1 MAESILCSLAGSIITKLGSFALQDLGLLWGFHDELDKLKGTVSALEAVLLDAEEKQSKSR 60
MA+SIL ++A ++ITKLGS AL++LG LWG +DEL KL+ +SA++AVLLDAEE+QS S
Sbjct: 27 MADSILFNVAANVITKLGSSALRELGSLWGVNDELGKLQNILSAIKAVLLDAEEQQSVSH 86
Query: 61 AVKDWILKLKDTFYDIDDLLDVFSYESLKRQVMTKHRTNNTKKVRIFFSKSNQIAFRLKM 120
AVKDWI KL+D FYD+DDL+D FSYE+L+RQV+TK RT TK+V IFFSKSNQ++F KM
Sbjct: 87 AVKDWISKLRDVFYDVDDLIDEFSYETLRRQVLTKDRTI-TKQVCIFFSKSNQVSFGHKM 146
Query: 121 SQKIKRVREKLDAIAMDKTQFNLYENTREIQDDESTKRLETTSFIREGEIIGRDDDKKSI 180
SQKIK+VREKLDAIA DKTQ +L RE +DDE K ET SFI +GE+IGRDDDKK+I
Sbjct: 147 SQKIKQVREKLDAIANDKTQLHLSVRMRETRDDELRKMRETCSFIPKGEVIGRDDDKKAI 206
Query: 181 IHYLLDTNIHEDSVAVIAIIGMGGLGKTALVQSIYGDEKVKKHFELTMWVCISEEFDVKV 240
I +LLDTN ED+V V++I+GMGGLGKTA+ QS+Y DEK+ +HF+L +WVCIS+EFD+KV
Sbjct: 207 IDFLLDTNTMEDNVEVVSIVGMGGLGKTAVAQSVYNDEKINEHFKLKLWVCISQEFDIKV 266
Query: 241 IIEKIIESLTKKKREPDLQLDTLQSMVREKIDGKRYLLVMDDVWNVNRAKWISLKRYLMG 300
I+EKIIE + KKK + LQLD LQSM++EKIDGK+YLLVMDDVWN + W+SLKR+LMG
Sbjct: 267 IVEKIIEFIAKKKPD-SLQLDILQSMLQEKIDGKKYLLVMDDVWNESHETWVSLKRFLMG 326
Query: 301 GAKGSRILITTRTHQVAQTFETILSHHLKELDEEKSWKLFRKMAFSNESEVLENSKLVVI 360
GAKGSRILITTR QVAQ +T+ HHLKELD E SW LFRKMAF NE E +ENS V I
Sbjct: 327 GAKGSRILITTRNLQVAQASDTVQFHHLKELDNESSWALFRKMAFLNEEEEIENSNKVRI 386
Query: 361 GKEIVTKLKGSPLAIRVIGSYLYSKKSEKDWLSFKDHELDTIMQQENEIQSILKISFNHL 420
GKEI+ KLKGSPL IR++G LY K +E DWLSFKD++L TI+QQEN+IQ ILKISFNHL
Sbjct: 387 GKEIIAKLKGSPLTIRIVGRLLYFKNTEMDWLSFKDNDLGTILQQENQIQPILKISFNHL 446
Query: 421 SSSLKHCFTYCALFSKDYHYEIRKNDLIKQWMAQGFIQPHNKKAMEDVGDDYFEELLGRS 480
S+LKHCFTYCALF KD YE +K+ L+KQWMAQGFIQ H+ K +EDVGDDYF+ELLGRS
Sbjct: 447 PSNLKHCFTYCALFPKD--YEFQKDGLVKQWMAQGFIQSHSNKEIEDVGDDYFKELLGRS 506
Query: 481 FFQDIRKNKWGEIKKFKMHDIIHDLACSVVENDCVLANDDTKSIDKRTRHVSISAFNSMT 540
FF +++ NKWG++K+ KMHD+IHDLAC +VEN+CV A+D TKSIDKRTRHVS + S
Sbjct: 507 FFHNVKVNKWGDVKECKMHDLIHDLACWIVENECVDASDKTKSIDKRTRHVSFPSNYSRK 566
Query: 541 RWKLITKSLIEAKNLRTLNYARRHHIDLSNHLRLRTLNL---EFHFVPKCIGKMKHLRYI 600
W+L KSL E KNLRTL+ + NHLRLR+LNL +F +PK I +++HLRY+
Sbjct: 567 SWELEAKSLTEVKNLRTLH--GPPFLLSENHLRLRSLNLGYSKFQKIPKFISQLRHLRYL 626
Query: 601 NITYCYIDFLPKAVTKLYHLETLIIRGCLELRELSSDIKNLINLRHLDIKDFKHVWSYMP 660
+I+ + FLPK +TKLY+LETLI+R C +LREL +DI NLINL+HLD+ + ++MP
Sbjct: 627 DISDHDMKFLPKFITKLYNLETLILRHCSDLRELPTDINNLINLKHLDVHGCYRL-THMP 686
Query: 661 KGMGSMTTLQTMNLFILGENKGGELSELNGLVNLRGSLSIQQLQFCKPIGLENVKYLEEK 720
KG+G +T+LQTMNLF+LG++KG +LSELN L LRGSL I+ L+ C L+N KY+EEK
Sbjct: 687 KGLGGLTSLQTMNLFVLGKDKGCDLSELNELARLRGSLLIKGLELCTTTDLKNAKYMEEK 746
Query: 721 SRIQKLELHWKTYQRESKID----DEDERVLESLKPHSNLQKIRIEGYRGLKLCNWFSFD 780
IQKL+L W +++ D ++DERVL+ LKPHSN+ K++I GYRG+KLCNW SFD
Sbjct: 747 FGIQKLKLRWNRDLYDAETDYASENDDERVLDCLKPHSNVHKMQIRGYRGVKLCNWLSFD 806
Query: 781 SIVNLVFIKLFNCEKLQQLPRFDRFPFLKHLHLEDLPSIEYIAINNYVSSSMTTFFPSLE 840
+ LV I+L +CEKLQ LP+FD+FPFLKHL LE+LPSIEYI NN +SSS TFFPSLE
Sbjct: 807 YLGGLVNIELQSCEKLQHLPQFDQFPFLKHLLLENLPSIEYIDNNNSLSSS--TFFPSLE 866
Query: 841 NLSIIKLPNLKEWWKG----ESIDQNTSFPTILRHLSQLKIHYCRQLASIPQHGPLQSLD 900
L+I+ +PNLK WWKG ES + FPTIL HLS+L I C QLASIPQH PL+SL
Sbjct: 867 KLTIMTMPNLKGWWKGETPPESARYSALFPTILHHLSRLDISNCPQLASIPQHPPLRSLA 926
Query: 901 IRDISLQLFELVIKMTAT--------------------NIIFLPNDLFSNVTHLQSLVIG 960
+ D+S+QLF++VIKM T ++ FLP +LF + T L+ +
Sbjct: 927 LNDVSVQLFDMVIKMATTPAADSSSALSKLSILHIQNIDLEFLPEELFGSTTDLEIFTVV 986
Query: 961 RCFNLKMSF------DDDNVRWKELGSLRTLRLCFIPKLEYLPKGFQYLKALEHLELLWC 1020
C NL+MS D+D V K+LG+L +L + +P+LEYL K +Y+ LE L+L C
Sbjct: 987 NCKNLQMSSSHLVDEDNDGVLGKKLGNLHSLGIFDMPQLEYLWKELKYMTTLERLDLYNC 1046
Query: 1021 ENLACILGIEHLTSLSRLEISNCPNLTSLPEGMTQLISLTCLIIDDCPNLSTLPEGLHHL 1044
N+ + GI HLTSLS L I NC NLTSLPEG++ L SL+ L I CPNL++LP G+ HL
Sbjct: 1047 PNIVSLEGISHLTSLSSLRICNCSNLTSLPEGISHLTSLSYLTIVCCPNLTSLPAGIGHL 1097
The following BLAST results are available for this feature:
| Match Name | E-value | Identity | Description | |
|---|
| RGA2_SOLBU | 5.1e-145 | 34.19 | Disease resistance protein RGA2 OS=Solanum bulbocastanum GN=RGA2 PE=1 SV=1 | [more] |
| RGA3_SOLBU | 2.0e-141 | 33.75 | Putative disease resistance protein RGA3 OS=Solanum bulbocastanum GN=RGA3 PE=2 S... | [more] |
| RGA1_SOLBU | 2.3e-137 | 33.37 | Putative disease resistance protein RGA1 OS=Solanum bulbocastanum GN=RGA1 PE=2 S... | [more] |
| RGA4_SOLBU | 3.3e-136 | 32.88 | Putative disease resistance protein RGA4 OS=Solanum bulbocastanum GN=RGA4 PE=2 S... | [more] |
| R13L1_ARATH | 1.5e-120 | 30.23 | Putative disease resistance RPP13-like protein 1 OS=Arabidopsis thaliana GN=RPPL... | [more] |
| Match Name | E-value | Identity | Description | |
|---|
| A0A0A0KIT2_CUCSA | 0.0e+00 | 90.45 | Uncharacterized protein OS=Cucumis sativus GN=Csa_6G375730 PE=3 SV=1 | [more] |
| A0A0A0KIT2_CUCSA | 9.8e-103 | 82.14 | Uncharacterized protein OS=Cucumis sativus GN=Csa_6G375730 PE=3 SV=1 | [more] |
| A0A0A0K5Z6_CUCSA | 3.0e-208 | 56.15 | Uncharacterized protein OS=Cucumis sativus GN=Csa_7G425920 PE=4 SV=1 | [more] |
| A0A0A0K5Z6_CUCSA | 7.0e-16 | 29.50 | Uncharacterized protein OS=Cucumis sativus GN=Csa_7G425920 PE=4 SV=1 | [more] |
| W9R509_9ROSA | 1.8e+00 | 46.94 | Putative disease resistance protein RGA4 OS=Morus notabilis GN=L484_023797 PE=3 ... | [more] |
| Match Name | E-value | Identity | Description | |
|---|
| AT3G14470.1 | 8.5e-122 | 30.23 | NB-ARC domain-containing disease resistance protein | [more] |
| AT3G14460.1 | 3.2e-97 | 29.29 | LRR and NB-ARC domains-containing disease resistance protein | [more] |
| AT3G07040.1 | 4.0e-71 | 26.85 | NB-ARC domain-containing disease resistance protein | [more] |
| AT3G46730.1 | 1.3e-69 | 29.97 | NB-ARC domain-containing disease resistance protein | [more] |
| AT1G58848.1 | 8.6e-66 | 25.51 | Disease resistance protein (CC-NBS-LRR class) family | [more] |
