Cucsa.301830 (gene) Cucumber (Gy14) v1

BLAST of Cucsa.301830 vs. Swiss-Prot
Match: ANM16_ARATH (Protein arginine N-methyltransferase 1.6 OS=Arabidopsis thaliana GN=PRMT16 PE=2 SV=2)

HSP 1 Score: 815.1 bits (2104), Expect = 5.9e-235
Identity = 415/700 (59.29%), Postives = 506/700 (72.29%), Query Frame = 1

Query: 1   MSSESTQRLFQLRFDPLTGNSEWVVIEEEAEGVPENSQKPLLATTSYLDMLNDSTRNRAF 60
           MSS+S+QR+FQLR DPLTGNSEW+VIE+  +  P  S   LLATTSYLDMLNDS RN A+
Sbjct: 33  MSSQSSQRVFQLRQDPLTGNSEWIVIEDNDQ--PGTSTDGLLATTSYLDMLNDSRRNIAY 92

Query: 61  CEAIDKTISKPCHVLDIGAGTGLLSMMAARAMDSVIGDCKGMVTACESYLPMVKLMRKVL 120
             AI+KTI++PCHVLDIGAGTGLLSMMA RAM    GD KGMVTACESYLPMVKLMRKV+
Sbjct: 93  RLAIEKTITEPCHVLDIGAGTGLLSMMAVRAMR---GDSKGMVTACESYLPMVKLMRKVM 152

Query: 121 RLNGMERKVKVINKRSDEFQFGA-DFTSRADALVSEILDSELLGEGLIPTLQHAHDKLLV 180
             NGM + + +INKRSDE + G+ D  SRAD LVSEILDSELLGEGLIP+LQHAHD LLV
Sbjct: 153 HKNGMTKNINLINKRSDELKVGSEDIASRADVLVSEILDSELLGEGLIPSLQHAHDMLLV 212

Query: 181 ENPLTVPYRATIYGQLVESTYLWKLHDLRNNEAKACDGIHLVPVG-DPIICVKPQQYAMH 240
           +NP TVPYRAT Y QLVEST+L  L DLRNNEAK  DG+ LVP G + +  +K QQY+MH
Sbjct: 213 DNPKTVPYRATTYCQLVESTFLCNLQDLRNNEAKTSDGVRLVPPGLESLFGIKSQQYSMH 272

Query: 241 CDAIANEVKLLSEPFRVFEFDFWKRPESHAENEVHVKATDSGRVHAVVSWWTLQLDREGT 300
            DAI  E+KLLSEP ++FEFDFWKRPES+ E +VH++A  +G VHA++SWW LQLD EGT
Sbjct: 273 VDAIEKEIKLLSEPVKIFEFDFWKRPESNGELDVHIEAKTTGSVHAIISWWVLQLDSEGT 332

Query: 301 IFYSTAPKWISPPYNAGAGDWCDHWKQCVWFIPGNGVSISKEEKVHLCASHNDTTFSYHL 360
           IFYSTAP+WI      G  DWCDHWKQCVWF PG GVSISK EKVHL ASH  T   Y+L
Sbjct: 333 IFYSTAPRWIDSNSEIGVRDWCDHWKQCVWFTPGTGVSISKGEKVHLHASHTCTNILYNL 392

Query: 361 -KAQIPGGEILKHGVNAENFKLILPPERIAVYGDREWRLAMLTAIKTALQGRAPSACMIA 420
            K Q    E     ++  +  L LPPER+A+YGD  +R ++  A + ALQG++   C++ 
Sbjct: 393 KKTQSLTHERTHFPLSTGDLHLTLPPERVAIYGDSIYRQSLFEATRKALQGKSYPQCLVI 452

Query: 421 DDSVFLTLMVACLSKKTLVLSLFPGIREKGTKYLQAVSRVNGICMDSI------------ 480
           DDS+ L LM   +S ++ VLSL PG++E   +Y +A++  NG   D              
Sbjct: 453 DDSLLLPLMALHISNRSRVLSLSPGLQENAARYFEAIADSNGFSKDRFEYFRDGKTNLAK 512

Query: 481 ----KVDLLISEPFYYGNDNALPWHNLRFWKERTMLDPVLSDDVLIMPSKGILRACFMSL 540
               K+DLLI EP+Y G +N LPW NLRFWK+RT+LD VLS+D ++MP KG+LR C M L
Sbjct: 513 AYPGKIDLLIGEPYYSGLENGLPWQNLRFWKDRTLLDSVLSEDAVVMPYKGVLRGCAMYL 572

Query: 541 PDLWSSRRRLGTIEGFDHSVTNDTLGACGKSPEGQEGPFLAFYIWQCGEHEELSEIFTLM 600
           PDLW SR  LG++EGFDH++ N TLG CG  P G++ P L F+IWQCGE + LS+ FT+M
Sbjct: 573 PDLWKSRCCLGSVEGFDHTLVNTTLGGCGDLPSGKDSPCLPFFIWQCGETKILSKEFTVM 632

Query: 601 EFDFSKPIS-PCSGKSQVKVTKAGICHGIVLWIDWLIDSKNSIVISTGPDKRYWKQGVKL 660
           EFDFSKPI+ PCSG+ Q++  K G+CHGI LW+DW++D +NS VISTGPD +YWKQGVKL
Sbjct: 633 EFDFSKPITGPCSGEVQIEFIKPGVCHGIALWMDWVMDEENSTVISTGPDDKYWKQGVKL 692

Query: 661 LADPVAVGPRDSGEGIECCSAAIEASFD-PSTGELELRHS 680
           L  PV V  R  G      S  I+AS D  S  EL + H+
Sbjct: 693 LGKPVTV--RMEGPS---SSIGIQASLDLSSNSELIVTHT 722

BLAST of Cucsa.301830 vs. Swiss-Prot
Match: ANM7_ORYSI (Protein arginine N-methyltransferase 7 OS=Oryza sativa subsp. indica GN=PRMT7 PE=3 SV=3)

HSP 1 Score: 768.5 bits (1983), Expect = 6.3e-221
Identity = 381/699 (54.51%), Postives = 491/699 (70.24%), Query Frame = 1

Query: 8   RLFQLRFDPLTGNSEWVVIEEEAEGV--PENSQKPLLATTSYLDMLNDSTRNRAFCEAID 67
           R FQLR +PLTG+SEW+V+EEE E    P    K LLATTSYLDMLNDS RNRA+  AI+
Sbjct: 29  RAFQLRLNPLTGDSEWLVVEEEEEEDHHPTPPPKQLLATTSYLDMLNDSARNRAYRRAIE 88

Query: 68  KTISKPC-HVLDIGAGTGLLSMMAARAMDSVIGDCKG-MVTACESYLPMVKLMRKVLRLN 127
             ++ P   VLDIGAGTGLLSMMAARA+ +V G+ +G  V+ACESYLPM KLMR+VLR N
Sbjct: 89  AAVTDPSSRVLDIGAGTGLLSMMAARALAAVGGETRGGSVSACESYLPMGKLMRRVLRAN 148

Query: 128 GMERKVKVINKRSDEFQFGADFTSRADALVSEILDSELLGEGLIPTLQHAHDKLLVENPL 187
           GME +VKV +KRSDE + G D  S AD LVSEILDSELLGEGLIPTLQ A+D LL +NP 
Sbjct: 149 GMENRVKVFHKRSDELKVGDDLDSPADILVSEILDSELLGEGLIPTLQQAYDMLLAKNPK 208

Query: 188 TVPYRATIYGQLVESTYLWKLHDLRNNEAKACDGIHLVPVG-DPIICVKPQQYAMHCDAI 247
            VPYRAT YGQLVEST+LWKLHDL NNEA A DG+ L P   + I+ VKPQQ+AM CDA+
Sbjct: 209 IVPYRATTYGQLVESTFLWKLHDLHNNEANAADGVWLTPGEMERIVSVKPQQHAMQCDAL 268

Query: 248 ANEVKLLSEPFRVFEFDFWKRPESHAENEVHVKATDSGRVHAVVSWWTLQLDREGTIFYS 307
            +E++LLSEPF+VFEFDFWKRP+SH E  + ++ T  G VHA++SWW LQLD  G+IFYS
Sbjct: 269 EDEIRLLSEPFKVFEFDFWKRPDSHREANIKIQTTRDGYVHAIISWWVLQLDSAGSIFYS 328

Query: 308 TAPKWISPPYNAGAG----DWCDHWKQCVWFIPGNGVSISKEEKVHLCASHNDTTFSYHL 367
           TAP+W     + G      DWCDHWKQCVWF+ G G+  ++++ + L A HN T+ SY L
Sbjct: 329 TAPRWARQSSSEGPQRDMKDWCDHWKQCVWFMQGKGIPATEDQVLSLRARHNQTSISYQL 388

Query: 368 KAQIPGGEILKHGVNAENFKLILPPERIAVYGDREWRLAMLTAIKTALQGRAPSACMIAD 427
                  +    G +     L L PERIA+YGD++WR A++  IK AL  ++   C++AD
Sbjct: 389 NINDEACDRSSKGDH-----LTLLPERIALYGDKDWRSALINTIKNALTVKSSPTCVVAD 448

Query: 428 DSVFLTLMVACLSKKTLVLSLFPGIREKGTKYLQAVSRVNGICMDSI------------- 487
           DS+FL L+++ +S  + V++++PG+R+KG  YL++V+  N   +D I             
Sbjct: 449 DSMFLALLISSMSPTSKVIAMYPGLRDKGAAYLRSVADANNFSIDQIQVIGKRASSITAD 508

Query: 488 -----KVDLLISEPFYYGNDNALPWHNLRFWKERTMLDPVLSDDVLIMPSKGILRACFMS 547
                KV+LL+ EPFY G++  LPW NLRFW  RT+LD +LS+D  IMP KGIL+ C MS
Sbjct: 509 DLKHKKVNLLVGEPFYLGSEGMLPWQNLRFWSVRTLLDSMLSEDAFIMPCKGILKLCAMS 568

Query: 548 LPDLWSSRRRLGTIEGFDHSVTNDTLGACGKSPEGQEGPFLAFYIWQCGEHEELSEIFTL 607
           LPDLW SR  L  +EGFDHSV N+TLGACG  P  Q+GP L +Y+WQCG  ++LS++++L
Sbjct: 569 LPDLWRSRSSLKDVEGFDHSVVNETLGACGYLPGDQQGPCLPYYVWQCGYTKKLSKVYSL 628

Query: 608 MEFDFSKPISPCSGKSQVKVTKAGICHGIVLWIDWLIDSKNSIVISTGPDKRYWKQGVKL 667
           M+F+FS+PI  C GK++++ +  G CHG  +WIDW++D + S+V++TGPD RYWKQGV+L
Sbjct: 629 MDFNFSEPIHSCFGKTKIEFSHDGTCHGFAVWIDWVLDERKSVVLTTGPDNRYWKQGVQL 688

Query: 668 LADPVAVGPRDSGEGIECCSAAIEASFDPSTGELELRHS 680
              PV V P  S          +EASFDPSTGE+    S
Sbjct: 689 FGKPVEVNPGKS-------VMHVEASFDPSTGEITFSSS 715

BLAST of Cucsa.301830 vs. Swiss-Prot
Match: ANM7_ORYSJ (Protein arginine N-methyltransferase 7 OS=Oryza sativa subsp. japonica GN=PRMT7 PE=2 SV=1)

HSP 1 Score: 765.8 bits (1976), Expect = 4.1e-220
Identity = 380/699 (54.36%), Postives = 491/699 (70.24%), Query Frame = 1

Query: 8   RLFQLRFDPLTGNSEWVVIEEEAEGV--PENSQKPLLATTSYLDMLNDSTRNRAFCEAID 67
           R FQLR +PLTG+SEW+V+EEE E    P    K LLATTSYLDMLNDS RNRA+  AI+
Sbjct: 29  RAFQLRLNPLTGDSEWLVVEEEEEEDHHPTPPPKQLLATTSYLDMLNDSARNRAYRRAIE 88

Query: 68  KTISKPC-HVLDIGAGTGLLSMMAARAMDSVIGDCKG-MVTACESYLPMVKLMRKVLRLN 127
             ++ P   VLDIGAGTGLLSMMAARA+ +V G+ +G  V+ACESYLPM KLMR+VLR N
Sbjct: 89  AAVTDPSSRVLDIGAGTGLLSMMAARALAAVGGETRGGSVSACESYLPMGKLMRRVLRAN 148

Query: 128 GMERKVKVINKRSDEFQFGADFTSRADALVSEILDSELLGEGLIPTLQHAHDKLLVENPL 187
           GME +VKV +KRSDE +   D  S AD LVSEILDSELLGEGLIPTLQ A+D LL +NP 
Sbjct: 149 GMENRVKVFHKRSDELKVRDDLDSPADILVSEILDSELLGEGLIPTLQQAYDMLLAKNPK 208

Query: 188 TVPYRATIYGQLVESTYLWKLHDLRNNEAKACDGIHLVPVG-DPIICVKPQQYAMHCDAI 247
            VPYRAT YGQLVEST+LWKLHDL NNEA A DG+ L P   + I+ VKPQQ+AM CDA+
Sbjct: 209 IVPYRATTYGQLVESTFLWKLHDLHNNEANAADGVWLTPGEMERIVSVKPQQHAMQCDAL 268

Query: 248 ANEVKLLSEPFRVFEFDFWKRPESHAENEVHVKATDSGRVHAVVSWWTLQLDREGTIFYS 307
            +E++LLSEPF+VFEFDFWKRP+SH E  + ++ T  G VHA++SWW LQLD  G+IFYS
Sbjct: 269 EDEIRLLSEPFKVFEFDFWKRPDSHREANIKIRTTRDGYVHAIISWWVLQLDSAGSIFYS 328

Query: 308 TAPKWISPPYNAGAG----DWCDHWKQCVWFIPGNGVSISKEEKVHLCASHNDTTFSYHL 367
           TAP+W     + G      DWCDHWKQCVWF+ G G+  ++++ + L A HN T+ SY L
Sbjct: 329 TAPRWARQSSSEGPQRDMKDWCDHWKQCVWFMQGKGIPATEDQVLSLRARHNQTSISYQL 388

Query: 368 KAQIPGGEILKHGVNAENFKLILPPERIAVYGDREWRLAMLTAIKTALQGRAPSACMIAD 427
                  +    G +     L L PERIA+YGD++WR A++  IK AL  ++   C++AD
Sbjct: 389 NINDEACDRSSKGDH-----LTLLPERIALYGDKDWRSALINTIKNALTVKSSPTCVVAD 448

Query: 428 DSVFLTLMVACLSKKTLVLSLFPGIREKGTKYLQAVSRVNGICMDSI------------- 487
           DS+FL L+++ +S  + V++++PG+R+KG  YL++V+  N   +D I             
Sbjct: 449 DSMFLALLISSMSPTSKVIAMYPGLRDKGAAYLRSVADANNFSIDQIQVIGKRASSITAD 508

Query: 488 -----KVDLLISEPFYYGNDNALPWHNLRFWKERTMLDPVLSDDVLIMPSKGILRACFMS 547
                KV+LL+ EPFY G++  LPW NLRFW  RT+LD +LS+D  IMP KGIL+ C MS
Sbjct: 509 DLKHKKVNLLVGEPFYLGSEGMLPWQNLRFWSVRTLLDSMLSEDAFIMPCKGILKLCAMS 568

Query: 548 LPDLWSSRRRLGTIEGFDHSVTNDTLGACGKSPEGQEGPFLAFYIWQCGEHEELSEIFTL 607
           LPDLW SR  L  +EGFDHSV N+TLGACG  P  Q+GP L +Y+WQCG  ++LS++++L
Sbjct: 569 LPDLWRSRSSLKDVEGFDHSVVNETLGACGCLPGDQQGPCLPYYVWQCGYTKKLSKVYSL 628

Query: 608 MEFDFSKPISPCSGKSQVKVTKAGICHGIVLWIDWLIDSKNSIVISTGPDKRYWKQGVKL 667
           M+F+FS+PI  C GK++++ +  G CHG  +WIDW++D + S+V++TGPD RYWKQGV+L
Sbjct: 629 MDFNFSEPIHSCFGKTKIEFSHDGTCHGFAVWIDWVLDERKSVVLTTGPDNRYWKQGVQL 688

Query: 668 LADPVAVGPRDSGEGIECCSAAIEASFDPSTGELELRHS 680
            + PV V P  S          +EASFDPSTGE+    S
Sbjct: 689 FSKPVEVNPGKS-------VMHVEASFDPSTGEITFSSS 715

BLAST of Cucsa.301830 vs. Swiss-Prot
Match: ANM7_HUMAN (Protein arginine N-methyltransferase 7 OS=Homo sapiens GN=PRMT7 PE=1 SV=1)

HSP 1 Score: 260.0 bits (663), Expect = 7.3e-68
Identity = 218/720 (30.28%), Postives = 328/720 (45.56%), Query Frame = 1

Query: 8   RLFQLRFDPLTGNSEWVVIEEEAEGVPENSQKPLLATTSYLDMLNDSTRNRAFCEAIDKT 67
           ++F  R +P TG+ EW+  +E  +   E      +A +SY DML+D  RN  + + I   
Sbjct: 2   KIFCSRANPTTGSVEWLEEDEHYDYHQE------IARSSYADMLHDKDRNVKYYQGIRAA 61

Query: 68  ISK------PCHVLDIGAGTGLLSMMAARAMDSVIGDCKGMVTACESYLPMVKLMRKVLR 127
           +S+         VLDIG GTGLLSMMA  A             A E + PM     K++ 
Sbjct: 62  VSRVKDRGQKALVLDIGTGTGLLSMMAVTAGAD-------FCYAIEVFKPMADAAVKIVE 121

Query: 128 LNGMERKVKVINKRSDEFQFG--ADFTSRADALVSEILDSELLGEGLIPTLQHAHDKLLV 187
            NG   K+KVINK S E   G   D   RA+ LV+E+ D+EL+GEG +P+ +HAH  L+ 
Sbjct: 122 KNGFSDKIKVINKHSTEVTVGPEGDMPCRANILVTELFDTELIGEGALPSYEHAHRHLVE 181

Query: 188 ENPLTVPYRATIYGQLVESTYLWKLHDLRNNEAKACDGIH-LVPVGDPIICVKPQQYAMH 247
           EN   VP+RAT+Y QLVES  +W  + L     +   G   +VP  D   C         
Sbjct: 182 ENCEAVPHRATVYAQLVESGRMWSWNKLFPIHVQTSLGEQVIVPPVDVESCPGAPSV--- 241

Query: 248 CDAIANEVK-----LLSEPFRVFEFDFWKRPESHAE-NEVHVKATDSGRVHAVVSWWTLQ 307
           CD   N+V      +LS+   +F  DF K+  S A  +    +   SGR   V+SWW ++
Sbjct: 242 CDIQLNQVSPADFTVLSDVLPMFSIDFSKQVSSSAACHSRRFEPLTSGRAQVVLSWWDIE 301

Query: 308 LDREGTIFYSTAPKWI-SPPYNAGAGDWCDHWKQCVWFIPGNGVSISKEEKVHLCASHND 367
           +D EG I  + AP W  S P       W DHW QCV+F+P     + +   ++L A H+D
Sbjct: 302 MDPEGKIKCTMAPFWAHSDPEEM---QWRDHWMQCVYFLP-QEEPVVQGSALYLVAHHDD 361

Query: 368 TTFSYHLKAQIP-GGEILK--HGVNAENFKLILPPERIAVYGDREWRLAMLTAIKTALQG 427
               Y L+   P   E ++    V      L+    R     D++     + A++T L  
Sbjct: 362 YCVWYSLQRTSPEKNERVRQMRPVCDCQAHLLWNRPRFGEINDQDRTDRYVQALRTVL-- 421

Query: 428 RAPSACMIADDSVFLTLMV------------ACLSKKTLVLSLFPG--IREKGTKYLQAV 487
           +  S C+   D   L+++             +  +   L+  +F    + +K     +  
Sbjct: 422 KPDSVCLCVSDGSLLSVLAHHLGVEQVFTVESSAASHKLLRKIFKANHLEDKINIIEKRP 481

Query: 488 SRVNGICMDSIKVDLLISEPFYYGNDNALPWHNLRFWKERTMLDPVLSDDVLIMPSKGIL 547
             +    +   KV LL+ EPF+    + LPWHNL FW  RT +D  L    ++MP    L
Sbjct: 482 ELLTNEDLQGRKVSLLLGEPFF--TTSLLPWHNLYFWYVRTAVDQHLGPGAMVMPQAASL 541

Query: 548 RACFMSLPDLWSSRRRLGTIEGFDHSVTNDTLGACGKSPEGQEGPFLAFYIWQCGEHEEL 607
            A  +   DLW  R   G  EGFD  + +D +       E +E      + + C     L
Sbjct: 542 HAVVVEFRDLWRIRSPCGDCEGFDVHIMDDMIKRALDFRESREAEPHPLWEYPC---RSL 601

Query: 608 SEIFTLMEFDFSKPI--SPCSGKSQVKVTKAGICHGIVLWIDWLIDSKNSIVISTG---- 667
           SE + ++ FDF +P+   P   +  V++ + G  H  VLW+++ +  +    +STG    
Sbjct: 602 SEPWQILTFDFQQPVPLQPLCAEGTVELRRPGQSHAAVLWMEYHLTPE--CTLSTGLLEP 661

Query: 668 --PD-----KRYWKQGVKLLADPVAVGPRDSGEGIECCSAAIEASFDPSTGE--LELRHS 680
             P+       + KQ V   +   A  PR    G    S A+E  F P TG+  +E RH+
Sbjct: 662 ADPEGGCCWNPHCKQAVYFFSP--APDPRALLGGPRTVSYAVE--FHPDTGDIIMEFRHA 688

BLAST of Cucsa.301830 vs. Swiss-Prot
Match: ANM7_DANRE (Protein arginine N-methyltransferase 7 OS=Danio rerio GN=prmt7 PE=2 SV=1)

HSP 1 Score: 257.7 bits (657), Expect = 3.6e-67
Identity = 216/718 (30.08%), Postives = 327/718 (45.54%), Query Frame = 1

Query: 13  RFDPLTGNSEWVVIEEEAEGVPENSQKPLLATTSYLDMLNDSTRNRAFCEAIDKTI---- 72
           R +P TG  +WV   EE +   E      +A + Y DML+D  RN  + E I   +    
Sbjct: 7   RANPTTGALDWVEESEEYDYHQE------IARSCYADMLHDKDRNEKYYEGIRAAVRRVK 66

Query: 73  ---SKPCHVLDIGAGTGLLSMMAARAMDSVIGDCKGMVTACESYLPMVKLMRKVLRLNGM 132
               +P  VLDIG GTGLLSMMA  A             A E + PM +    ++  NG 
Sbjct: 67  ARGERPV-VLDIGTGTGLLSMMAVTAGAD-------FCYAIEVFKPMAQAASCIVERNGF 126

Query: 133 ERKVKVINKRSDEFQFG--ADFTSRADALVSEILDSELLGEGLIPTLQHAHDKLLVENPL 192
             K+K+INK S E   G   D   RA+ LV+E+ D+EL+GEG +P+ +HAH  L+     
Sbjct: 127 SDKIKIINKHSTEVTVGPDGDMQERANILVTELFDTELIGEGALPSYEHAHMHLVQTGCE 186

Query: 193 TVPYRATIYGQLVESTYLWKLHDLRNNEAKACDGIHLVPVGDPIICVKPQQYAMHCDAIA 252
            VP+RATIY QLVES  LWK   +R  +    DG  L+P G    C         CD   
Sbjct: 187 AVPHRATIYAQLVESDMLWKWAQMRPIDV---DGHRLMPPGAVQECAGAPSV---CDIQL 246

Query: 253 NEV-----KLLSEPFRVFEFDFWKRPESHAEN-EVHVKATDSGRVHAVVSWWTLQLDREG 312
           ++V       +S    +F  DF K   S A++  V  K+   GR   V+SWW + +D EG
Sbjct: 247 SQVPTDAFTAISPVCTMFSVDFSKPVSSAAQSYTVRFKSQTGGRAQVVLSWWDIDMDPEG 306

Query: 313 TIFYSTAPKW-ISPPYNAGAGDWCDHWKQCVWFIPGNGVSISKEEKVHLCASHNDTTFSY 372
            I  + AP W  + P+   A  W DHW Q V+F+P    ++S+ E++ L  SH+D +  Y
Sbjct: 307 NIVCTMAPSWSYADPH---AYPWRDHWMQSVYFLPAE-ENVSEGEELMLMVSHDDYSLWY 366

Query: 373 HLKAQIPGGEILKHGVNAENFK--------LILPPERIAVYGDREWRLAMLTAIKTALQG 432
            L          ++ V    F+        L+    R     D +   + ++A+++ L  
Sbjct: 367 SLT------HSEQNDVRVAPFRPCCTCQAHLVWTRPRFGELNDEQRTESYVSALRSIL-- 426

Query: 433 RAPSACMIADDSVFLTLMVACL-SKKTLVLSLFPGIREKGTKYLQAVSRVNGI------- 492
           +  S C+   D   L +    L SKK   L      ++   + L   S  +G+       
Sbjct: 427 KPDSVCLSVSDGSLLPVFAHLLGSKKVFSLESSGMAKQVIEQVLHTNSLKDGVQLLGIRA 486

Query: 493 ------CMDSIKVDLLISEPFYYGNDNALPWHNLRFWKERTMLDPVLSDDVLIMPSKGIL 552
                  +D  ++ +L+ EP++  + + LPWH+L FW  RT +  +L  D  I+P    L
Sbjct: 487 EQLSLADLDGNQISVLMGEPYF--STSLLPWHSLFFWYCRTAVAQLLQPDATILPRAATL 546

Query: 553 RACFMSLPDLWSSRRRLGTIEGFDHSVTNDTLGACGKSPEGQEGPFLAFYIWQCGEHEEL 612
            A  +   DLW  R   GT EGFD S  ++ +       E  E      + + C     L
Sbjct: 547 YAVAVEFQDLWRIRFPCGTCEGFDVSPMDEMIQRSLDFRESWEAEPHPLWEYPC---RAL 606

Query: 613 SEIFTLMEFDFSK--PISPCSGKSQVKVTKAGICHGIVLWIDWLIDSKNSIVISTGPDKR 672
           ++   +M FDF++  P  P S    V  T  G CHG+ LW+++ +   + I +S G  K 
Sbjct: 607 TKPCPVMTFDFTQCVPEQPISSDGAVPFTGRGRCHGVALWMEYQL--TDDISVSMGLTKA 666

Query: 673 -----------YWKQGVKLLADPVAVGPRDSGEGIECCSAAIEASFDPSTGELELRHS 680
                      + KQGV             SG+G E  S ++  +F+P +G++++  S
Sbjct: 667 VSQEGACEWNPHRKQGVFFFRS----AKETSGDGREDLSYSL--TFEPHSGDIKMDFS 679

BLAST of Cucsa.301830 vs. TrEMBL
Match: A0A0A0KRA0_CUCSA (Uncharacterized protein OS=Cucumis sativus GN=Csa_5G162000 PE=3 SV=1)

HSP 1 Score: 1389.8 bits (3596), Expect = 0.0e+00
Identity = 681/699 (97.42%), Postives = 681/699 (97.42%), Query Frame = 1

Query: 1   MSSESTQRLFQLRFDPLTGNSEWVVIEEEAEGVPENSQKPLLATTSYLDMLNDSTRNRAF 60
           MSSESTQRLFQLRFDPLTGNSEWVVIEEEAEGVPENSQKPLLATTSYLDMLNDSTRNRAF
Sbjct: 40  MSSESTQRLFQLRFDPLTGNSEWVVIEEEAEGVPENSQKPLLATTSYLDMLNDSTRNRAF 99

Query: 61  CEAIDKTISKPCHVLDIGAGTGLLSMMAARAMDSVIGDCKGMVTACESYLPMVKLMRKVL 120
           CEAIDKTISKPCHVLDIGAGTGLLSMMAARAMDSVIGDCKGMVTACESYLPMVKLMRKVL
Sbjct: 100 CEAIDKTISKPCHVLDIGAGTGLLSMMAARAMDSVIGDCKGMVTACESYLPMVKLMRKVL 159

Query: 121 RLNGMERKVKVINKRSDEFQFGADFTSRADALVSEILDSELLGEGLIPTLQHAHDKLLVE 180
           RLNGMERKVKVINKRSDEFQFGADFTSRADALVSEILDSELLGEGLIPTLQHAHDKLLVE
Sbjct: 160 RLNGMERKVKVINKRSDEFQFGADFTSRADALVSEILDSELLGEGLIPTLQHAHDKLLVE 219

Query: 181 NPLTVPYRATIYGQLVESTYLWKLHDLRNNEAKACDGIHLVPVGDPIICVKPQQYAMHCD 240
           NPLTVPYRATIYGQLVESTYLWKLHDLRNNEAKACDGIHLVPVGDPIICVKPQQYAMHCD
Sbjct: 220 NPLTVPYRATIYGQLVESTYLWKLHDLRNNEAKACDGIHLVPVGDPIICVKPQQYAMHCD 279

Query: 241 AIANEVKLLSEPFRVFEFDFWKRPESHAENEVHVKATDSGRVHAVVSWWTLQLDREGTIF 300
           AIANEVKLLSEPFRVFEFDFWKRPESHAENEVHVKATDSGRVHAVVSWWTLQLDREGTIF
Sbjct: 280 AIANEVKLLSEPFRVFEFDFWKRPESHAENEVHVKATDSGRVHAVVSWWTLQLDREGTIF 339

Query: 301 YSTAPKWISPPYNAGAGDWCDHWKQCVWFIPGNGVSISKEEKVHLCASHNDTTFSYHLKA 360
           YSTAPKWISPPYNAGAGDWCDHWKQCVWFIPGNGVSISKEEKVHLCASHNDTTFSYHLKA
Sbjct: 340 YSTAPKWISPPYNAGAGDWCDHWKQCVWFIPGNGVSISKEEKVHLCASHNDTTFSYHLKA 399

Query: 361 QIPGGEILKHGVNAENFKLILPPERIAVYGDREWRLAMLTAIKTALQGRAPSACMIADDS 420
           QIPGGEILKHGVNAENFKLILPPERIAVYGDREWRLAMLTAIKTALQGRAPSACMIADDS
Sbjct: 400 QIPGGEILKHGVNAENFKLILPPERIAVYGDREWRLAMLTAIKTALQGRAPSACMIADDS 459

Query: 421 VFLTLMVACLSKKTLVLSLFPGIREKGTKYLQAVSRVNGICMDSI--------------- 480
           VFLTLMVACLSKKTLVLSLFPGIREKGTKYLQAVSRVNGICMDSI               
Sbjct: 460 VFLTLMVACLSKKTLVLSLFPGIREKGTKYLQAVSRVNGICMDSIKVIEKRKSCLTIHDT 519

Query: 481 ---KVDLLISEPFYYGNDNALPWHNLRFWKERTMLDPVLSDDVLIMPSKGILRACFMSLP 540
              KVDLLISEPFYYGNDNALPWHNLRFWKERTMLDPVLSDDVLIMPSKGILRACFMSLP
Sbjct: 520 FEKKVDLLISEPFYYGNDNALPWHNLRFWKERTMLDPVLSDDVLIMPSKGILRACFMSLP 579

Query: 541 DLWSSRRRLGTIEGFDHSVTNDTLGACGKSPEGQEGPFLAFYIWQCGEHEELSEIFTLME 600
           DLWSSRRRLGTIEGFDHSVTNDTLGACGKSPEGQEGPFLAFYIWQCGEHEELSEIFTLME
Sbjct: 580 DLWSSRRRLGTIEGFDHSVTNDTLGACGKSPEGQEGPFLAFYIWQCGEHEELSEIFTLME 639

Query: 601 FDFSKPISPCSGKSQVKVTKAGICHGIVLWIDWLIDSKNSIVISTGPDKRYWKQGVKLLA 660
           FDFSKPISPCSGKSQVKVTKAGICHGIVLWIDWLIDSKNSIVISTGPDKRYWKQGVKLLA
Sbjct: 640 FDFSKPISPCSGKSQVKVTKAGICHGIVLWIDWLIDSKNSIVISTGPDKRYWKQGVKLLA 699

Query: 661 DPVAVGPRDSGEGIECCSAAIEASFDPSTGELELRHSFL 682
           DPVAVGPRDSGEGIECCSAAIEASFDPSTGELELRHSFL
Sbjct: 700 DPVAVGPRDSGEGIECCSAAIEASFDPSTGELELRHSFL 738

BLAST of Cucsa.301830 vs. TrEMBL
Match: D7TN52_VITVI (Putative uncharacterized protein OS=Vitis vinifera GN=VIT_02s0154g00540 PE=3 SV=1)

HSP 1 Score: 958.7 bits (2477), Expect = 3.7e-276
Identity = 478/712 (67.13%), Postives = 555/712 (77.95%), Query Frame = 1

Query: 1   MSSESTQRLFQLRFDPLTGNSEWVVIEEEAEGVPENSQKPLLATTSYLDMLNDSTRNRAF 60
           MSS S QRLFQL+ DPLTGNSEWVVI+EE + V EN  +PLL+TTSYLDMLNDS RNRAF
Sbjct: 28  MSSSSAQRLFQLKLDPLTGNSEWVVIDEEDQ-VSENPSEPLLSTTSYLDMLNDSRRNRAF 87

Query: 61  CEAIDKTISKPCHVLDIGAGTGLLSMMAARAMDSVIGDC------KGMVTACESYLPMVK 120
            EAIDKT++K C VLDIGAGTGLLSMMAARAM S  GD       +GMVTACESYLPMVK
Sbjct: 88  REAIDKTVTKNCRVLDIGAGTGLLSMMAARAMGS--GDSVACPRTEGMVTACESYLPMVK 147

Query: 121 LMRKVLRLNGMERKVKVINKRSDEFQFGADFTSRADALVSEILDSELLGEGLIPTLQHAH 180
           LMRKVL LNGM RK+ VINKRSDE   G D TSRAD LVSEILDSELLGEGLIPTLQHAH
Sbjct: 148 LMRKVLHLNGMGRKINVINKRSDELNIGVDITSRADVLVSEILDSELLGEGLIPTLQHAH 207

Query: 181 DKLLVENPLTVPYRATIYGQLVESTYLWKLHDLRNNEAKACDGIHLVPVG-DPIICVKPQ 240
           D LLVEN  TVPYRAT YGQLVES +LWKLHDL NNEAKA D + LVP G + I+ +K Q
Sbjct: 208 DMLLVENAKTVPYRATTYGQLVESKFLWKLHDLYNNEAKALDNVCLVPAGQETILSIKQQ 267

Query: 241 QYAMHCDAIANEVKLLSEPFRVFEFDFWKRPESHAENEVHVKATDSGRVHAVVSWWTLQL 300
           QYAMHCDAI  E+KLLSEPF++FEFDF KRP+SH E E+H+KA D+G VHAVVSWW LQL
Sbjct: 268 QYAMHCDAIKEEIKLLSEPFKIFEFDFSKRPDSHEETELHIKAIDNGSVHAVVSWWILQL 327

Query: 301 DREGTIFYSTAPKWISPPYN--------AGAGDWCDHWKQCVWFIPGNGVSISKEEKVHL 360
           D EGTIFYSTAPKWIS P+N        + AGDWCDHWKQCVWFIPG G+ +SK E+VHL
Sbjct: 328 DCEGTIFYSTAPKWISVPFNINKSQTPFSSAGDWCDHWKQCVWFIPGKGIYVSKHEEVHL 387

Query: 361 CASHNDTTFSYHLKAQIPGGEILKHGVNAENFKLILPPERIAVYGDREWRLAMLTAIKTA 420
            A H D + SY+LK Q+   EI +H + A + +LIL PER+A+YGD EWRL+MLTAIK  
Sbjct: 388 HAIHTDISISYNLKTQLSRTEIGQHDLFARDSQLILSPERVAIYGDSEWRLSMLTAIKNT 447

Query: 421 LQGRAPSACMIADDSVFLTLMVACLSKKTLVLSLFPGIREKGTKYLQAVSRVNGICMDSI 480
           LQG+  S C++ DDS+FL +++A LS+ + V+SLFPG+R+KGT+YLQAV+ VNG  MD +
Sbjct: 448 LQGKVISLCVVTDDSIFLAILIAHLSRTSHVISLFPGLRDKGTQYLQAVADVNGFSMDRV 507

Query: 481 ------------------KVDLLISEPFYYGNDNALPWHNLRFWKERTMLDPVLSDDVLI 540
                             KVDLLI EPFYYGN+  LPW NLRFWKERTML+ VLS+DV+I
Sbjct: 508 EVLQNWKTCLTTLDTRQKKVDLLIGEPFYYGNEGMLPWQNLRFWKERTMLNSVLSEDVVI 567

Query: 541 MPSKGILRACFMSLPDLWSSRRRLGTIEGFDHSVTNDTLGACGKSPEGQEGPFLAFYIWQ 600
           MP KGILRAC MSLPDLW+SRR L  IEG DHSV N TLGACG  PE QEGP L +++WQ
Sbjct: 568 MPCKGILRACAMSLPDLWNSRRCLNKIEGLDHSVVNATLGACGDLPEAQEGPCLPYFVWQ 627

Query: 601 CGEHEELSEIFTLMEFDFSKPISPCSGKSQVKVTKAGICHGIVLWIDWLIDSKNSIVIST 660
           CGE +ELSEI T+MEFDFSKPI+PCSGK+ V+ T+ GICHG VLWIDW++D++N  V+ T
Sbjct: 628 CGEIKELSEILTVMEFDFSKPINPCSGKAMVEFTETGICHGFVLWIDWVMDAENCTVLPT 687

Query: 661 GPDKRYWKQGVKLLADPVAVGPRDSGEGIECCSAAIEASFDPSTGELELRHS 680
           GP  RYWKQGVKLL+ P++VG   S     C S  IEASFDPS+GEL ++H+
Sbjct: 688 GPVHRYWKQGVKLLSKPISVGFHGSESTSGCFSTEIEASFDPSSGELIVKHT 736

BLAST of Cucsa.301830 vs. TrEMBL
Match: B9GYA9_POPTR (Arginine N-methyltransferase family protein OS=Populus trichocarpa GN=POPTR_0003s07410g PE=3 SV=2)

HSP 1 Score: 944.9 bits (2441), Expect = 5.5e-272
Identity = 465/699 (66.52%), Postives = 546/699 (78.11%), Query Frame = 1

Query: 1   MSSESTQRLFQLRFDPLTGNSEWVVIEE---EAEGVPENSQKPLLATTSYLDMLNDSTRN 60
           MSS+STQR+FQL+ DPLTGNSEWV+IE+   E E   ++S   LLATTSYLDMLND TRN
Sbjct: 42  MSSDSTQRVFQLKLDPLTGNSEWVIIEDGNKEEEETFKSSSHALLATTSYLDMLNDDTRN 101

Query: 61  RAFCEAIDKTISKPCHVLDIGAGTGLLSMMAARAMDSVIGDCKGMVTACESYLPMVKLMR 120
           RAF EAIDKTI+KPCHVLDIGAGTGLLSMMAARAM +   + KGMVTACESYLPMVKLMR
Sbjct: 102 RAFREAIDKTITKPCHVLDIGAGTGLLSMMAARAMGTCDDNKKGMVTACESYLPMVKLMR 161

Query: 121 KVLRLNGMERKVKVINKRSDEFQFGADFTSRADALVSEILDSELLGEGLIPTLQHAHDKL 180
           KVL LNGM + VK+ NKRSDE Q   D  SRAD LVSEILDSELLGEGLIPTLQHAHD L
Sbjct: 162 KVLNLNGMGKNVKIFNKRSDELQVDIDIPSRADVLVSEILDSELLGEGLIPTLQHAHDML 221

Query: 181 LVENPLTVPYRATIYGQLVESTYLWKLHDLRNNEAKACDGIHLVPVG-DPIICVKPQQYA 240
           LV+NPLTVPYRAT YGQLVEST+LWKLHDL NNE +A DGI LVP G D I+CVK +Q+ 
Sbjct: 222 LVDNPLTVPYRATTYGQLVESTFLWKLHDLSNNEEEASDGIRLVPAGLDTILCVKAKQHP 281

Query: 241 MHCDAIANEVKLLSEPFRVFEFDFWKRPESHAENEVHVKATDSGRVHAVVSWWTLQLDRE 300
           MHCDAI+ E+ LLSEPF++FEFDFWKRP+S  ++++ +KAT+ GR+HAVVSWW LQLD E
Sbjct: 282 MHCDAISKEINLLSEPFKIFEFDFWKRPDSQGDSKLLIKATNDGRIHAVVSWWILQLDSE 341

Query: 301 GTIFYSTAPKWISPPYNAGAGDWCDHWKQCVWFIPGNGVSISKEEKVHLCASHNDTTFSY 360
           GTIFYSTAP+WI+ P     G+WCDHWKQCVWFIPG G+ ISK E+VHL A H+DT+ SY
Sbjct: 342 GTIFYSTAPRWITSPIATHTGNWCDHWKQCVWFIPGEGIPISKGEEVHLHAVHSDTSVSY 401

Query: 361 HLKAQIPGGEILKHGVNAENFKLILPPERIAVYGDREWRLAMLTAIKTALQGRAPSACMI 420
           +L  Q    EI +    A +  L L PERIA+YGDR+WR  MLTA+   LQ R    C++
Sbjct: 402 NLATQ--ATEIRECDSIAGDSHLTLSPERIAIYGDRKWRSCMLTALNNLLQARVHPLCVV 461

Query: 421 ADDSVFLTLMVACLSKKTLVLSLFPGIREKGTKYLQAVSRVNGICMDSI----------- 480
           ADDSVFL+L+VA LSK + V++LFPG+RE+G +YL+AV+  NGI  D I           
Sbjct: 462 ADDSVFLSLLVAHLSKTSNVIALFPGLRERGVQYLKAVADANGITADRIEVFQKKKYLTL 521

Query: 481 ------KVDLLISEPFYYGNDNALPWHNLRFWKERTMLDPVLSDDVLIMPSKGILRACFM 540
                 KVDLLI EP+YYGND  LPW N RFWKERTMLD VL++DVL+MP K +L+AC M
Sbjct: 522 NDTKQKKVDLLIGEPYYYGNDGMLPWQNFRFWKERTMLDSVLAEDVLVMPCKAMLKACAM 581

Query: 541 SLPDLWSSRRRLGTIEGFDHSVTNDTLGACGKSPEGQEGPFLAFYIWQCGEHEELSEIFT 600
           SLPDLW S R L  IE FDHS+ N+TLGACG  P   EGP L F+IWQCGE +ELSE FT
Sbjct: 582 SLPDLWKSHRCLSKIEDFDHSIVNNTLGACGDLPAPLEGPLLPFFIWQCGETKELSETFT 641

Query: 601 LMEFDFSKPISPCSGKSQVKVTKAGICHGIVLWIDWLIDSKNSIVISTGPDKRYWKQGVK 660
           +MEFDFSK I PC GK+QV+ T+ G+CHG VLWIDW++D+KNS+V++TGPD+RYWKQGVK
Sbjct: 642 IMEFDFSKSIGPCYGKAQVEFTEQGMCHGFVLWIDWVMDAKNSVVLTTGPDERYWKQGVK 701

Query: 661 LLADPVAVGPRDSGEGIECCSAAIEASFDPSTGELELRH 679
           LL+ PVAVG R S  G  CCS  IEASFDPS+GEL ++H
Sbjct: 702 LLSQPVAVGGRGSSTG-NCCSTLIEASFDPSSGELAVKH 737

BLAST of Cucsa.301830 vs. TrEMBL
Match: V4TJI5_9ROSI (Uncharacterized protein OS=Citrus clementina GN=CICLE_v10023597mg PE=3 SV=1)

HSP 1 Score: 927.2 bits (2395), Expect = 1.2e-266
Identity = 463/690 (67.10%), Postives = 541/690 (78.41%), Query Frame = 1

Query: 5   STQRLFQLRFDPLTGNSEWVVIEEEAEGVPENSQKPLLATTSYLDMLNDSTRNRAFCEAI 64
           STQR+FQL+ DPLTGNSEWVVIEE  E VPE+SQ+PLLATTSYLDMLNDS RNRA+  AI
Sbjct: 2   STQRMFQLKLDPLTGNSEWVVIEEN-EDVPESSQEPLLATTSYLDMLNDSYRNRAYRLAI 61

Query: 65  DKTISKPCHVLDIGAGTGLLSMMAARAMDSVIG----DCKGMVTACESYLPMVKLMRKVL 124
           DK ++K CHVLDIGAGTGLLSMMAARAM S       + KGMVTACESYLPMVKLM+KVL
Sbjct: 62  DKMVTKSCHVLDIGAGTGLLSMMAARAMGSSDSTTSLNTKGMVTACESYLPMVKLMKKVL 121

Query: 125 RLNGMERKVKVINKRSDEFQFGADFTSRADALVSEILDSELLGEGLIPTLQHAHDKLLVE 184
            +NGM R +KVINKRSDE + G D  SRAD LVSEILDSELLGEGLIPTLQHAHD+LLVE
Sbjct: 122 HVNGMGRNIKVINKRSDELEVGVDIDSRADILVSEILDSELLGEGLIPTLQHAHDRLLVE 181

Query: 185 NPLTVPYRATIYGQLVESTYLWKLHDLRNNEAKACDGIHLVPVG-DPIICVKPQQYAMHC 244
           NPLTVP R T YGQLVEST+LWKLHDL NNEAKA D IHLVP G D I+ VK QQYAMHC
Sbjct: 182 NPLTVPCRVTTYGQLVESTFLWKLHDLYNNEAKALDDIHLVPAGMDSILHVKSQQYAMHC 241

Query: 245 DAIANEVKLLSEPFRVFEFDFWKRPESHAENEVHVKATDSGRVHAVVSWWTLQLDREGTI 304
           DAI  E+KLLSEPF++FEFDFWKRP+SH E E+ +K+TD GRVHAVVSWW LQLDREGTI
Sbjct: 242 DAITKEIKLLSEPFKIFEFDFWKRPDSHGEAELQIKSTDDGRVHAVVSWWVLQLDREGTI 301

Query: 305 FYSTAPKWISPPYNAGAGDWCDHWKQCVWFIPGNGVSISKEEKVHLCASHNDTTFSYHLK 364
           FYSTAP+WIS P +   G+WCDHWKQCVWFIPG G+SI K E++   A H +T+ SY LK
Sbjct: 302 FYSTAPRWISLPIHKSTGNWCDHWKQCVWFIPGKGMSICKGEELLFHALHTETSVSYELK 361

Query: 365 AQIPGGEILKHGVNAENFKLILPPERIAVYGDREWRLAMLTAIKTALQGRAPSACMIADD 424
           +QIP  +  +H +NA++F+L LPPERIA+YGD EWRL+M+ A++ ALQGR    C++ADD
Sbjct: 362 SQIPITDERQHNLNAKDFQLALPPERIAIYGDGEWRLSMVMAMRNALQGRVQPLCVVADD 421

Query: 425 SVFLTLMVA-CLSKKTLV-LSLFP----GIREKGTKYLQAVSRVNGICMDSI---KVDLL 484
           SVFLT+ VA C   +T+   + F      I +KG K L          MD     KVDLL
Sbjct: 422 SVFLTICVARCQYLRTVADPNCFSIDRVEILQKGKKCLT---------MDDTQQKKVDLL 481

Query: 485 ISEPFYYGNDNALPWHNLRFWKERTMLDPVLSDDVLIMPSKGILRACFMSLPDLWSSRRR 544
           I EP+Y+GND  LPW NLRFWKER+ LDPVLS +V+IMP KGIL+AC +  PD+W+SRR 
Sbjct: 482 IGEPYYFGNDGMLPWQNLRFWKERSKLDPVLSKEVIIMPFKGILKACAIFCPDIWNSRRS 541

Query: 545 LGTIEGFDHSVTNDTLGACGKSPEGQEGPFLAFYIWQCGEHEELSEIFTLMEFDFSKPIS 604
           L  IEGFDH+V N TLGACG  P  +EGP L F+ WQCGE ++LS +FT+MEFDFSKPIS
Sbjct: 542 LSKIEGFDHAVVNTTLGACGDLPAPKEGPCLPFFTWQCGEIKKLSNVFTVMEFDFSKPIS 601

Query: 605 PCSGKSQVKVTKAGICHGIVLWIDWLIDSKNSIVISTGPDKRYWKQGVKLLADPVAVGPR 664
           PC GK QV+ T+ G+CHG  LWIDW++DS+NSI+ISTGPDKRYWKQGVKL+A PVAVG  
Sbjct: 602 PCQGKVQVEFTELGLCHGFALWIDWVMDSENSIIISTGPDKRYWKQGVKLMAKPVAVGFE 661

Query: 665 DSGEGIECCSAAIEASFDPSTGELELRHSF 681
            SG    C S  +EASF+PS GEL ++H+F
Sbjct: 662 GSGRTDLCSSTLVEASFNPSNGELNVQHTF 681

BLAST of Cucsa.301830 vs. TrEMBL
Match: A0A0D2QM67_GOSRA (Uncharacterized protein OS=Gossypium raimondii GN=B456_003G171100 PE=3 SV=1)

HSP 1 Score: 926.0 bits (2392), Expect = 2.6e-266
Identity = 449/700 (64.14%), Postives = 537/700 (76.71%), Query Frame = 1

Query: 1   MSSESTQRLFQLRFDPLTGNSEWVVIEEEAEGVPENSQKPLLATTSYLDMLNDSTRNRAF 60
           MSS+S+ RLFQL+ DPLTGNSEWVVIE+  + +P+  ++PLLATTSYLDMLNDS RN+AF
Sbjct: 39  MSSDSSHRLFQLKLDPLTGNSEWVVIEDN-DDLPDCPKEPLLATTSYLDMLNDSCRNKAF 98

Query: 61  CEAIDKTISKPCHVLDIGAGTGLLSMMAARAMDSVIGDCKGMVTACESYLPMVKLMRKVL 120
             AI++T++KPCHVLDIGAGTGLLSMMAARAM        G VTACESYLPM KLMRKVL
Sbjct: 99  RSAIERTVTKPCHVLDIGAGTGLLSMMAARAMG-----LNGTVTACESYLPMAKLMRKVL 158

Query: 121 RLNGMERKVKVINKRSDEFQFGADFTSRADALVSEILDSELLGEGLIPTLQHAHDKLLVE 180
             N M + + + NKRSDE + G D  SRAD LVSEILDSELLGEGLIPTLQHA+DKLLVE
Sbjct: 159 HRNRMGKAISLFNKRSDELEVGVDIPSRADVLVSEILDSELLGEGLIPTLQHAYDKLLVE 218

Query: 181 NPLTVPYRATIYGQLVESTYLWKLHDLRNNEAKACDGIHLVPVG-DPIICVKPQQYAMHC 240
           NPLTVP+RA  YGQLVESTYLWKLHDL   EAK  DGIHLVP G D II VK +QY MHC
Sbjct: 219 NPLTVPFRAVTYGQLVESTYLWKLHDLSGIEAKVSDGIHLVPTGSDSIIQVKSRQYPMHC 278

Query: 241 DAIANEVKLLSEPFRVFEFDFWKRPESHAENEVHVKATDSGRVHAVVSWWTLQLDREGTI 300
           DAI  E+KLLSEPF +FEFDFWKRPES+ E +V VK  D G +HA+VSWW LQLDREGT+
Sbjct: 279 DAIREEIKLLSEPFEIFEFDFWKRPESNGETKVQVKVIDDGNIHAIVSWWVLQLDREGTV 338

Query: 301 FYSTAPKWISPPYNAGAGDWCDHWKQCVWFIPGNGVSISKEEKVHLCASHNDTTFSYHLK 360
           FYSTAP+WI+ P N G  DWCDHWKQCVWF  G G+S+S+ E++ L ASH +T+ SY+L 
Sbjct: 339 FYSTAPRWINSPANIGYRDWCDHWKQCVWFFRGKGMSVSQGEELLLEASHTETSVSYNLN 398

Query: 361 AQIPGGEILKHGVNAENFKLILPPERIAVYGDREWRLAMLTAIKTALQGRAPSACMIADD 420
            Q+P  +  +H  N  +F+L+L PERIA+YGD +WR ++L A++ ALQGR    C++ DD
Sbjct: 399 VQVPKNDTRQHDHNIRDFQLLLSPERIAIYGDSKWRSSVLAAVRNALQGRVNPLCVVVDD 458

Query: 421 SVFLTLMVACLSKKTLVLSLFPGIREKGTKYLQAVSRVNGICMDSI-------------- 480
           S+FLTL+ A LSK + VLSLFPG+REKG +YL+ V + N   MD +              
Sbjct: 459 SIFLTLLAANLSKTSHVLSLFPGLREKGAQYLETVVKANCFSMDRVEVPEKRKPCLTLHD 518

Query: 481 ----KVDLLISEPFYYGNDNALPWHNLRFWKERTMLDPVLSDDVLIMPSKGILRACFMSL 540
               KVD+LI EP+YY N+  LPW NLRFWK+RT+LDPVLS+D LIMP KGIL+AC MSL
Sbjct: 519 TRGKKVDMLIGEPYYYANEGMLPWKNLRFWKDRTLLDPVLSEDALIMPCKGILKACAMSL 578

Query: 541 PDLWSSRRRLGTIEGFDHSVTNDTLGACGKSPEGQEGPFLAFYIWQCGEHEELSEIFTLM 600
           PDLWSSR  LG +EGFDHS+ N TLGACG+ P  +EGPFL F IWQCGE +ELSEIFT++
Sbjct: 579 PDLWSSRCCLGDVEGFDHSIVNTTLGACGELPAPKEGPFLPFSIWQCGEIKELSEIFTIL 638

Query: 601 EFDFSKPISPCSGKSQVKVTKAGICHGIVLWIDWLIDSKNSIVISTGPDKRYWKQGVKLL 660
           EFDFSKPIS C G +QVK T+ G+CHG VLW+DW++D+ NSIVISTGP+ RYWKQGVKLL
Sbjct: 639 EFDFSKPISSCHGTAQVKFTEGGVCHGFVLWVDWIMDTDNSIVISTGPEHRYWKQGVKLL 698

Query: 661 ADPVAVGPRDSGEGIECCSAAIEASFDPSTGELELRHSFL 682
           + PVAVG        E  S  +EA F+PS+ EL L+H+FL
Sbjct: 699 SKPVAVGINGCNSNSEYGSTIVEALFEPSSSELILKHAFL 732

BLAST of Cucsa.301830 vs. TAIR10
Match: AT4G16570.1 (AT4G16570.1 protein arginine methyltransferase 7)

HSP 1 Score: 815.1 bits (2104), Expect = 3.3e-236
Identity = 415/700 (59.29%), Postives = 506/700 (72.29%), Query Frame = 1

Query: 1   MSSESTQRLFQLRFDPLTGNSEWVVIEEEAEGVPENSQKPLLATTSYLDMLNDSTRNRAF 60
           MSS+S+QR+FQLR DPLTGNSEW+VIE+  +  P  S   LLATTSYLDMLNDS RN A+
Sbjct: 33  MSSQSSQRVFQLRQDPLTGNSEWIVIEDNDQ--PGTSTDGLLATTSYLDMLNDSRRNIAY 92

Query: 61  CEAIDKTISKPCHVLDIGAGTGLLSMMAARAMDSVIGDCKGMVTACESYLPMVKLMRKVL 120
             AI+KTI++PCHVLDIGAGTGLLSMMA RAM    GD KGMVTACESYLPMVKLMRKV+
Sbjct: 93  RLAIEKTITEPCHVLDIGAGTGLLSMMAVRAMR---GDSKGMVTACESYLPMVKLMRKVM 152

Query: 121 RLNGMERKVKVINKRSDEFQFGA-DFTSRADALVSEILDSELLGEGLIPTLQHAHDKLLV 180
             NGM + + +INKRSDE + G+ D  SRAD LVSEILDSELLGEGLIP+LQHAHD LLV
Sbjct: 153 HKNGMTKNINLINKRSDELKVGSEDIASRADVLVSEILDSELLGEGLIPSLQHAHDMLLV 212

Query: 181 ENPLTVPYRATIYGQLVESTYLWKLHDLRNNEAKACDGIHLVPVG-DPIICVKPQQYAMH 240
           +NP TVPYRAT Y QLVEST+L  L DLRNNEAK  DG+ LVP G + +  +K QQY+MH
Sbjct: 213 DNPKTVPYRATTYCQLVESTFLCNLQDLRNNEAKTSDGVRLVPPGLESLFGIKSQQYSMH 272

Query: 241 CDAIANEVKLLSEPFRVFEFDFWKRPESHAENEVHVKATDSGRVHAVVSWWTLQLDREGT 300
            DAI  E+KLLSEP ++FEFDFWKRPES+ E +VH++A  +G VHA++SWW LQLD EGT
Sbjct: 273 VDAIEKEIKLLSEPVKIFEFDFWKRPESNGELDVHIEAKTTGSVHAIISWWVLQLDSEGT 332

Query: 301 IFYSTAPKWISPPYNAGAGDWCDHWKQCVWFIPGNGVSISKEEKVHLCASHNDTTFSYHL 360
           IFYSTAP+WI      G  DWCDHWKQCVWF PG GVSISK EKVHL ASH  T   Y+L
Sbjct: 333 IFYSTAPRWIDSNSEIGVRDWCDHWKQCVWFTPGTGVSISKGEKVHLHASHTCTNILYNL 392

Query: 361 -KAQIPGGEILKHGVNAENFKLILPPERIAVYGDREWRLAMLTAIKTALQGRAPSACMIA 420
            K Q    E     ++  +  L LPPER+A+YGD  +R ++  A + ALQG++   C++ 
Sbjct: 393 KKTQSLTHERTHFPLSTGDLHLTLPPERVAIYGDSIYRQSLFEATRKALQGKSYPQCLVI 452

Query: 421 DDSVFLTLMVACLSKKTLVLSLFPGIREKGTKYLQAVSRVNGICMDSI------------ 480
           DDS+ L LM   +S ++ VLSL PG++E   +Y +A++  NG   D              
Sbjct: 453 DDSLLLPLMALHISNRSRVLSLSPGLQENAARYFEAIADSNGFSKDRFEYFRDGKTNLAK 512

Query: 481 ----KVDLLISEPFYYGNDNALPWHNLRFWKERTMLDPVLSDDVLIMPSKGILRACFMSL 540
               K+DLLI EP+Y G +N LPW NLRFWK+RT+LD VLS+D ++MP KG+LR C M L
Sbjct: 513 AYPGKIDLLIGEPYYSGLENGLPWQNLRFWKDRTLLDSVLSEDAVVMPYKGVLRGCAMYL 572

Query: 541 PDLWSSRRRLGTIEGFDHSVTNDTLGACGKSPEGQEGPFLAFYIWQCGEHEELSEIFTLM 600
           PDLW SR  LG++EGFDH++ N TLG CG  P G++ P L F+IWQCGE + LS+ FT+M
Sbjct: 573 PDLWKSRCCLGSVEGFDHTLVNTTLGGCGDLPSGKDSPCLPFFIWQCGETKILSKEFTVM 632

Query: 601 EFDFSKPIS-PCSGKSQVKVTKAGICHGIVLWIDWLIDSKNSIVISTGPDKRYWKQGVKL 660
           EFDFSKPI+ PCSG+ Q++  K G+CHGI LW+DW++D +NS VISTGPD +YWKQGVKL
Sbjct: 633 EFDFSKPITGPCSGEVQIEFIKPGVCHGIALWMDWVMDEENSTVISTGPDDKYWKQGVKL 692

Query: 661 LADPVAVGPRDSGEGIECCSAAIEASFD-PSTGELELRHS 680
           L  PV V  R  G      S  I+AS D  S  EL + H+
Sbjct: 693 LGKPVTV--RMEGPS---SSIGIQASLDLSSNSELIVTHT 722

BLAST of Cucsa.301830 vs. TAIR10
Match: AT4G29510.1 (AT4G29510.1 arginine methyltransferase 11)

HSP 1 Score: 68.2 bits (165), Expect = 2.3e-11
Identity = 63/287 (21.95%), Postives = 128/287 (44.60%), Query Frame = 1

Query: 49  DMLNDSTRNRAFCEAI--DKTISKPCHVLDIGAGTGLLSMMAARAMDSVIGDCKGMVTAC 108
           +ML D  R + +   I  +K + K   VLD+GAGTG+LS+  A+A     G        C
Sbjct: 84  EMLKDVVRTKTYQNVIYQNKFLIKDKIVLDVGAGTGILSLFCAKA-----GAAHVYAVEC 143

Query: 109 ESYLPMVKLMRKVLRLNGMERKVKVINKRSDEFQFGADFTSRADALVSEILDSELLGEGL 168
                M K   ++++ NG    + V+  + +E +     T + D ++SE +   LL E +
Sbjct: 144 SQMADMAK---EIVKANGFSDVITVLKGKIEEIELP---TPKVDVIISEWMGYFLLFENM 203

Query: 169 IPTLQHAHDKLLVENPLTVPYRATIYGQLVESTYLWKLHDLRNNEAKACDGIHLVPVGDP 228
           + ++ +A DK LVE  + +P +A+++   +E +      + + ++ +  + ++    G  
Sbjct: 204 LDSVLYARDKWLVEGGVVLPDKASLHLTAIEDS------EYKEDKIEFWNSVY----GFD 263

Query: 229 IICVKPQQYAMHCDAIANEVKLLSEPFRVFEFDFWKRPESHAENEVHVK--ATDSGRVHA 288
           + C+K +          ++ +++++   +   D  K     A      K  A  +  +HA
Sbjct: 264 MSCIKKKAMMEPLVDTVDQNQIVTDSRLLKTMDISKMSSGDASFTAPFKLVAQRNDYIHA 323

Query: 289 VVSWWTLQLDR-EGTIFYSTAPKWISPPYNAGAGDWCDHWKQCVWFI 331
           +V+++ +        + +ST PK               HWKQ V ++
Sbjct: 324 LVAYFDVSFTMCHKLLGFSTGPK-----------SRATHWKQTVLYL 338

BLAST of Cucsa.301830 vs. TAIR10
Match: AT2G19670.1 (AT2G19670.1 protein arginine methyltransferase 1A)

HSP 1 Score: 67.4 bits (163), Expect = 3.9e-11
Identity = 62/287 (21.60%), Postives = 128/287 (44.60%), Query Frame = 1

Query: 49  DMLNDSTRNRAFCEAI--DKTISKPCHVLDIGAGTGLLSMMAARAMDSVIGDCKGMVTAC 108
           +ML D  R +++ + I  +K + K   VLD+GAGTG+LS+  A+A     G        C
Sbjct: 60  EMLKDVVRTKSYQDVIYKNKFLIKDKIVLDVGAGTGILSLFCAKA-----GAAHVYAVEC 119

Query: 109 ESYLPMVKLMRKVLRLNGMERKVKVINKRSDEFQFGADFTSRADALVSEILDSELLGEGL 168
                M    +++++ NG    + V+  + +E +       + D ++SE +   LL E +
Sbjct: 120 SQ---MADTAKEIVKSNGFSDVITVLKGKIEEIELPVP---KVDVIISEWMGYFLLYENM 179

Query: 169 IPTLQHAHDKLLVENPLTVPYRATIYGQLVESTYLWKLHDLRNNEAKACDGIHLVPVGDP 228
           + T+ +A +K LV+  + +P +A++Y   +E  +       ++++ +  D ++    G  
Sbjct: 180 LDTVLYARNKWLVDGGIVLPDKASLYVTAIEDAH------YKDDKVEFWDDVY----GFD 239

Query: 229 IICVKPQQYAMHCDAIANEVKLLSEPFRVFEFDFWKRPESHAENEVHVK--ATDSGRVHA 288
           + C+K +          +  +++++   +   D  K     A      K  A  +  +HA
Sbjct: 240 MSCIKRRAITEPLVDTVDGNQIVTDSKLLKTMDISKMAAGDASFTAPFKLVAQRNDHIHA 299

Query: 289 VVSWWTLQLDR-EGTIFYSTAPKWISPPYNAGAGDWCDHWKQCVWFI 331
           +V+++ +        + +ST PK               HWKQ V ++
Sbjct: 300 LVAYFDVSFTMCHKKMGFSTGPK-----------SRATHWKQTVLYL 314

BLAST of Cucsa.301830 vs. TAIR10
Match: AT3G20020.1 (AT3G20020.1 protein arginine methyltransferase 6)

HSP 1 Score: 52.4 bits (124), Expect = 1.3e-06
Identity = 38/146 (26.03%), Postives = 73/146 (50.00%), Query Frame = 1

Query: 49  DMLNDSTRNRAFCEAI--DKTISKPCHVLDIGAGTGLLSMMAARAMDSVIGDCKGMVTAC 108
           +M+ D  R   + EAI   +++ +   V+D+G GTG+LS+  A+A    +        A 
Sbjct: 95  EMIKDRARTETYREAIMQHQSLIEGKVVVDVGCGTGILSIFCAQAGAKRVYAVDASDIAV 154

Query: 109 ESYLPMVKLMRKVLRLNGMERKVKVINKRSDEFQFGADFTSRADALVSEILDSELLGEGL 168
           ++        ++V++ NG+  KV V++ R ++     +     D ++SE +   LL E +
Sbjct: 155 QA--------KEVVKANGLSDKVIVLHGRVED----VEIDEEVDVIISEWMGYMLLYESM 214

Query: 169 IPTLQHAHDKLLVENPLTVPYRATIY 193
           + ++  A D+ L    L +P  AT+Y
Sbjct: 215 LGSVITARDRWLKPGGLILPSHATLY 228

BLAST of Cucsa.301830 vs. TAIR10
Match: AT3G12270.1 (AT3G12270.1 protein arginine methyltransferase 3)

HSP 1 Score: 50.1 bits (118), Expect = 6.4e-06
Identity = 43/153 (28.10%), Postives = 74/153 (48.37%), Query Frame = 1

Query: 49  DMLNDSTRNRAFCEAIDK--TISKPCHVLDIGAGTGLLSMMAARAMDSVIGDCKGMVTAC 108
           +ML+D  R  A+ +A+ K  T+     V+D+G GTG+LS+ AA+A  S        V A 
Sbjct: 257 EMLSDKVRTEAYRDALLKNPTLLNGSVVMDVGCGTGILSLFAAKAGAS-------RVVAV 316

Query: 109 ESYLPMVKLMRKVLRLNGM------ERKVKVINKRSDEFQFGADFTSRA-DALVSEILDS 168
           E+   M K+  K+ + N +         ++V +   +E          + D LVSE +  
Sbjct: 317 EASEKMAKVATKIAKDNKVFNDNEHNGVLEVAHSMVEELDKSIQIQPHSVDVLVSEWMGY 376

Query: 169 ELLGEGLIPTLQHAHDKLLVENPLTVPYRATIY 193
            LL E ++ ++ +A D+ L      +P  AT++
Sbjct: 377 CLLYESMLSSVLYARDRWLKPGGAILPDTATMF 402

HSP 2 Score: 38.5 bits (88), Expect = 1.9e-02
Identity = 19/54 (35.19%), Postives = 28/54 (51.85%), Query Frame = 1

Query: 602 KAGICHGIVLWIDWLIDSK----NSIVISTGP--DKRYWKQGVKLLADPVAVGP 650
           K  +CHG+VLW D    S+    N  V+ST P     +W Q +    +P++V P
Sbjct: 485 KTRLCHGVVLWFDTGFTSRFCKENPTVLSTSPYTPPTHWAQTILTFQEPISVAP 538

BLAST of Cucsa.301830 vs. NCBI nr
Match: gi|449469390|ref|XP_004152403.1| (PREDICTED: protein arginine N-methyltransferase 1.6 isoform X1 [Cucumis sativus])

HSP 1 Score: 1389.8 bits (3596), Expect = 0.0e+00
Identity = 681/699 (97.42%), Postives = 681/699 (97.42%), Query Frame = 1

Query: 1   MSSESTQRLFQLRFDPLTGNSEWVVIEEEAEGVPENSQKPLLATTSYLDMLNDSTRNRAF 60
           MSSESTQRLFQLRFDPLTGNSEWVVIEEEAEGVPENSQKPLLATTSYLDMLNDSTRNRAF
Sbjct: 40  MSSESTQRLFQLRFDPLTGNSEWVVIEEEAEGVPENSQKPLLATTSYLDMLNDSTRNRAF 99

Query: 61  CEAIDKTISKPCHVLDIGAGTGLLSMMAARAMDSVIGDCKGMVTACESYLPMVKLMRKVL 120
           CEAIDKTISKPCHVLDIGAGTGLLSMMAARAMDSVIGDCKGMVTACESYLPMVKLMRKVL
Sbjct: 100 CEAIDKTISKPCHVLDIGAGTGLLSMMAARAMDSVIGDCKGMVTACESYLPMVKLMRKVL 159

Query: 121 RLNGMERKVKVINKRSDEFQFGADFTSRADALVSEILDSELLGEGLIPTLQHAHDKLLVE 180
           RLNGMERKVKVINKRSDEFQFGADFTSRADALVSEILDSELLGEGLIPTLQHAHDKLLVE
Sbjct: 160 RLNGMERKVKVINKRSDEFQFGADFTSRADALVSEILDSELLGEGLIPTLQHAHDKLLVE 219

Query: 181 NPLTVPYRATIYGQLVESTYLWKLHDLRNNEAKACDGIHLVPVGDPIICVKPQQYAMHCD 240
           NPLTVPYRATIYGQLVESTYLWKLHDLRNNEAKACDGIHLVPVGDPIICVKPQQYAMHCD
Sbjct: 220 NPLTVPYRATIYGQLVESTYLWKLHDLRNNEAKACDGIHLVPVGDPIICVKPQQYAMHCD 279

Query: 241 AIANEVKLLSEPFRVFEFDFWKRPESHAENEVHVKATDSGRVHAVVSWWTLQLDREGTIF 300
           AIANEVKLLSEPFRVFEFDFWKRPESHAENEVHVKATDSGRVHAVVSWWTLQLDREGTIF
Sbjct: 280 AIANEVKLLSEPFRVFEFDFWKRPESHAENEVHVKATDSGRVHAVVSWWTLQLDREGTIF 339

Query: 301 YSTAPKWISPPYNAGAGDWCDHWKQCVWFIPGNGVSISKEEKVHLCASHNDTTFSYHLKA 360
           YSTAPKWISPPYNAGAGDWCDHWKQCVWFIPGNGVSISKEEKVHLCASHNDTTFSYHLKA
Sbjct: 340 YSTAPKWISPPYNAGAGDWCDHWKQCVWFIPGNGVSISKEEKVHLCASHNDTTFSYHLKA 399

Query: 361 QIPGGEILKHGVNAENFKLILPPERIAVYGDREWRLAMLTAIKTALQGRAPSACMIADDS 420
           QIPGGEILKHGVNAENFKLILPPERIAVYGDREWRLAMLTAIKTALQGRAPSACMIADDS
Sbjct: 400 QIPGGEILKHGVNAENFKLILPPERIAVYGDREWRLAMLTAIKTALQGRAPSACMIADDS 459

Query: 421 VFLTLMVACLSKKTLVLSLFPGIREKGTKYLQAVSRVNGICMDSI--------------- 480
           VFLTLMVACLSKKTLVLSLFPGIREKGTKYLQAVSRVNGICMDSI               
Sbjct: 460 VFLTLMVACLSKKTLVLSLFPGIREKGTKYLQAVSRVNGICMDSIKVIEKRKSCLTIHDT 519

Query: 481 ---KVDLLISEPFYYGNDNALPWHNLRFWKERTMLDPVLSDDVLIMPSKGILRACFMSLP 540
              KVDLLISEPFYYGNDNALPWHNLRFWKERTMLDPVLSDDVLIMPSKGILRACFMSLP
Sbjct: 520 FEKKVDLLISEPFYYGNDNALPWHNLRFWKERTMLDPVLSDDVLIMPSKGILRACFMSLP 579

Query: 541 DLWSSRRRLGTIEGFDHSVTNDTLGACGKSPEGQEGPFLAFYIWQCGEHEELSEIFTLME 600
           DLWSSRRRLGTIEGFDHSVTNDTLGACGKSPEGQEGPFLAFYIWQCGEHEELSEIFTLME
Sbjct: 580 DLWSSRRRLGTIEGFDHSVTNDTLGACGKSPEGQEGPFLAFYIWQCGEHEELSEIFTLME 639

Query: 601 FDFSKPISPCSGKSQVKVTKAGICHGIVLWIDWLIDSKNSIVISTGPDKRYWKQGVKLLA 660
           FDFSKPISPCSGKSQVKVTKAGICHGIVLWIDWLIDSKNSIVISTGPDKRYWKQGVKLLA
Sbjct: 640 FDFSKPISPCSGKSQVKVTKAGICHGIVLWIDWLIDSKNSIVISTGPDKRYWKQGVKLLA 699

Query: 661 DPVAVGPRDSGEGIECCSAAIEASFDPSTGELELRHSFL 682
           DPVAVGPRDSGEGIECCSAAIEASFDPSTGELELRHSFL
Sbjct: 700 DPVAVGPRDSGEGIECCSAAIEASFDPSTGELELRHSFL 738

BLAST of Cucsa.301830 vs. NCBI nr
Match: gi|659073479|ref|XP_008437081.1| (PREDICTED: protein arginine N-methyltransferase 1.6 [Cucumis melo])

HSP 1 Score: 1338.6 bits (3463), Expect = 0.0e+00
Identity = 655/699 (93.71%), Postives = 666/699 (95.28%), Query Frame = 1

Query: 1   MSSESTQRLFQLRFDPLTGNSEWVVIEEEAEGVPENSQKPLLATTSYLDMLNDSTRNRAF 60
           MSSESTQRLFQLRFDPLTGNSEWVVIEEE EGV ENSQKPLLATTSYLDMLNDSTRNRAF
Sbjct: 40  MSSESTQRLFQLRFDPLTGNSEWVVIEEEVEGVLENSQKPLLATTSYLDMLNDSTRNRAF 99

Query: 61  CEAIDKTISKPCHVLDIGAGTGLLSMMAARAMDSVIGDCKGMVTACESYLPMVKLMRKVL 120
            EAIDKT+SKPCHVLDIGAGTGLLSMMAARAMDSV+GDCKGMVTACESYLPMVKLMRKVL
Sbjct: 100 REAIDKTLSKPCHVLDIGAGTGLLSMMAARAMDSVMGDCKGMVTACESYLPMVKLMRKVL 159

Query: 121 RLNGMERKVKVINKRSDEFQFGADFTSRADALVSEILDSELLGEGLIPTLQHAHDKLLVE 180
           RLNGMERKVKVINKRSDEFQFGAD TSRADALVSEILDSELLGEGLIPTLQHAHDKLLVE
Sbjct: 160 RLNGMERKVKVINKRSDEFQFGADLTSRADALVSEILDSELLGEGLIPTLQHAHDKLLVE 219

Query: 181 NPLTVPYRATIYGQLVESTYLWKLHDLRNNEAKACDGIHLVPVGDPIICVKPQQYAMHCD 240
           NPLTVPYRAT+YGQLVESTYLWKLHDL NNEAKACDGIHLVPVGDPIICVKPQQYAMHCD
Sbjct: 220 NPLTVPYRATVYGQLVESTYLWKLHDLYNNEAKACDGIHLVPVGDPIICVKPQQYAMHCD 279

Query: 241 AIANEVKLLSEPFRVFEFDFWKRPESHAENEVHVKATDSGRVHAVVSWWTLQLDREGTIF 300
           AIANEVKLLSEPFRVFEFDFWKRPESHAENE+HVKATDSGR+HAVVSWWTLQLDREGTIF
Sbjct: 280 AIANEVKLLSEPFRVFEFDFWKRPESHAENELHVKATDSGRIHAVVSWWTLQLDREGTIF 339

Query: 301 YSTAPKWISPPYNAGAGDWCDHWKQCVWFIPGNGVSISKEEKVHLCASHNDTTFSYHLKA 360
           YSTAPKWISPPYN GAG WCDHWKQCVWFIPGNGVSISKEEKVHLCAS NDTTFSYHLK+
Sbjct: 340 YSTAPKWISPPYNVGAGGWCDHWKQCVWFIPGNGVSISKEEKVHLCASRNDTTFSYHLKS 399

Query: 361 QIPGGEILKHGVNAENFKLILPPERIAVYGDREWRLAMLTAIKTALQGRAPSACMIADDS 420
           QIPGGEIL+HGVNAENFKLILPPERIAVYGDREWRLAMLTAIKT LQGRAPS C+IADDS
Sbjct: 400 QIPGGEILQHGVNAENFKLILPPERIAVYGDREWRLAMLTAIKTELQGRAPSVCVIADDS 459

Query: 421 VFLTLMVACLSKKTLVLSLFPGIREKGTKYLQAVSRVNGICMDSI--------------- 480
           VFLTLMVACLSK TLVLSLFPGIREKGTKYLQAVS VNGICMDSI               
Sbjct: 460 VFLTLMVACLSKTTLVLSLFPGIREKGTKYLQAVSHVNGICMDSIKVIEKRKSCLTIQDT 519

Query: 481 ---KVDLLISEPFYYGNDNALPWHNLRFWKERTMLDPVLSDDVLIMPSKGILRACFMSLP 540
              KVDLLISEPFYYGNDNALPWHNLRFWKERTMLD VLSDDVLIMPSKGILRACFMSLP
Sbjct: 520 FEKKVDLLISEPFYYGNDNALPWHNLRFWKERTMLDQVLSDDVLIMPSKGILRACFMSLP 579

Query: 541 DLWSSRRRLGTIEGFDHSVTNDTLGACGKSPEGQEGPFLAFYIWQCGEHEELSEIFTLME 600
           DLWSSRRRLGTIEGFDHSVTNDTLGACGK PEGQEGPFLAFYIWQCGE+EELSEIFTLME
Sbjct: 580 DLWSSRRRLGTIEGFDHSVTNDTLGACGKLPEGQEGPFLAFYIWQCGEYEELSEIFTLME 639

Query: 601 FDFSKPISPCSGKSQVKVTKAGICHGIVLWIDWLIDSKNSIVISTGPDKRYWKQGVKLLA 660
           FDFSKPISPCSGKSQVKVTKAGICHGIVLWIDW+IDSKNSIVISTGPDKRYWKQGVKLLA
Sbjct: 640 FDFSKPISPCSGKSQVKVTKAGICHGIVLWIDWVIDSKNSIVISTGPDKRYWKQGVKLLA 699

Query: 661 DPVAVGPRDSGEGIECCSAAIEASFDPSTGELELRHSFL 682
           DPVAV PRDSGEGIECCSAAIEASFDPSTGEL+LRHSFL
Sbjct: 700 DPVAVEPRDSGEGIECCSAAIEASFDPSTGELDLRHSFL 738

BLAST of Cucsa.301830 vs. NCBI nr
Match: gi|778700057|ref|XP_011654801.1| (PREDICTED: protein arginine N-methyltransferase 1.6 isoform X2 [Cucumis sativus])

HSP 1 Score: 1088.6 bits (2814), Expect = 0.0e+00
Identity = 527/545 (96.70%), Postives = 527/545 (96.70%), Query Frame = 1

Query: 155 EILDSELLGEGLIPTLQHAHDKLLVENPLTVPYRATIYGQLVESTYLWKLHDLRNNEAKA 214
           EILDSELLGEGLIPTLQHAHDKLLVENPLTVPYRATIYGQLVESTYLWKLHDLRNNEAKA
Sbjct: 17  EILDSELLGEGLIPTLQHAHDKLLVENPLTVPYRATIYGQLVESTYLWKLHDLRNNEAKA 76

Query: 215 CDGIHLVPVGDPIICVKPQQYAMHCDAIANEVKLLSEPFRVFEFDFWKRPESHAENEVHV 274
           CDGIHLVPVGDPIICVKPQQYAMHCDAIANEVKLLSEPFRVFEFDFWKRPESHAENEVHV
Sbjct: 77  CDGIHLVPVGDPIICVKPQQYAMHCDAIANEVKLLSEPFRVFEFDFWKRPESHAENEVHV 136

Query: 275 KATDSGRVHAVVSWWTLQLDREGTIFYSTAPKWISPPYNAGAGDWCDHWKQCVWFIPGNG 334
           KATDSGRVHAVVSWWTLQLDREGTIFYSTAPKWISPPYNAGAGDWCDHWKQCVWFIPGNG
Sbjct: 137 KATDSGRVHAVVSWWTLQLDREGTIFYSTAPKWISPPYNAGAGDWCDHWKQCVWFIPGNG 196

Query: 335 VSISKEEKVHLCASHNDTTFSYHLKAQIPGGEILKHGVNAENFKLILPPERIAVYGDREW 394
           VSISKEEKVHLCASHNDTTFSYHLKAQIPGGEILKHGVNAENFKLILPPERIAVYGDREW
Sbjct: 197 VSISKEEKVHLCASHNDTTFSYHLKAQIPGGEILKHGVNAENFKLILPPERIAVYGDREW 256

Query: 395 RLAMLTAIKTALQGRAPSACMIADDSVFLTLMVACLSKKTLVLSLFPGIREKGTKYLQAV 454
           RLAMLTAIKTALQGRAPSACMIADDSVFLTLMVACLSKKTLVLSLFPGIREKGTKYLQAV
Sbjct: 257 RLAMLTAIKTALQGRAPSACMIADDSVFLTLMVACLSKKTLVLSLFPGIREKGTKYLQAV 316

Query: 455 SRVNGICMDSI------------------KVDLLISEPFYYGNDNALPWHNLRFWKERTM 514
           SRVNGICMDSI                  KVDLLISEPFYYGNDNALPWHNLRFWKERTM
Sbjct: 317 SRVNGICMDSIKVIEKRKSCLTIHDTFEKKVDLLISEPFYYGNDNALPWHNLRFWKERTM 376

Query: 515 LDPVLSDDVLIMPSKGILRACFMSLPDLWSSRRRLGTIEGFDHSVTNDTLGACGKSPEGQ 574
           LDPVLSDDVLIMPSKGILRACFMSLPDLWSSRRRLGTIEGFDHSVTNDTLGACGKSPEGQ
Sbjct: 377 LDPVLSDDVLIMPSKGILRACFMSLPDLWSSRRRLGTIEGFDHSVTNDTLGACGKSPEGQ 436

Query: 575 EGPFLAFYIWQCGEHEELSEIFTLMEFDFSKPISPCSGKSQVKVTKAGICHGIVLWIDWL 634
           EGPFLAFYIWQCGEHEELSEIFTLMEFDFSKPISPCSGKSQVKVTKAGICHGIVLWIDWL
Sbjct: 437 EGPFLAFYIWQCGEHEELSEIFTLMEFDFSKPISPCSGKSQVKVTKAGICHGIVLWIDWL 496

Query: 635 IDSKNSIVISTGPDKRYWKQGVKLLADPVAVGPRDSGEGIECCSAAIEASFDPSTGELEL 682
           IDSKNSIVISTGPDKRYWKQGVKLLADPVAVGPRDSGEGIECCSAAIEASFDPSTGELEL
Sbjct: 497 IDSKNSIVISTGPDKRYWKQGVKLLADPVAVGPRDSGEGIECCSAAIEASFDPSTGELEL 556

BLAST of Cucsa.301830 vs. NCBI nr
Match: gi|568846738|ref|XP_006477200.1| (PREDICTED: protein arginine N-methyltransferase 1.6 isoform X2 [Citrus sinensis])

HSP 1 Score: 966.1 bits (2496), Expect = 3.3e-278
Identity = 472/699 (67.53%), Postives = 554/699 (79.26%), Query Frame = 1

Query: 5   STQRLFQLRFDPLTGNSEWVVIEEEAEGVPENSQKPLLATTSYLDMLNDSTRNRAFCEAI 64
           STQR+FQL+ DPLTGNSEWVVIEE  E VPE+SQ+PLLATTSYLDMLNDS RNRA+  AI
Sbjct: 37  STQRMFQLKLDPLTGNSEWVVIEEN-EDVPESSQEPLLATTSYLDMLNDSYRNRAYRLAI 96

Query: 65  DKTISKPCHVLDIGAGTGLLSMMAARAMDSVIG----DCKGMVTACESYLPMVKLMRKVL 124
           DK ++K CHVLDIGAGTGLLSMMAARAM S       + KGMVTACESYLPMVKLM+KVL
Sbjct: 97  DKMVTKSCHVLDIGAGTGLLSMMAARAMGSSDSTTSLNTKGMVTACESYLPMVKLMKKVL 156

Query: 125 RLNGMERKVKVINKRSDEFQFGADFTSRADALVSEILDSELLGEGLIPTLQHAHDKLLVE 184
            +NGM R +KVINKRSDE + G D  SRAD LVSEILDSELLGEGLIPTLQHAHD+LLVE
Sbjct: 157 HVNGMGRNIKVINKRSDELEVGVDIDSRADILVSEILDSELLGEGLIPTLQHAHDRLLVE 216

Query: 185 NPLTVPYRATIYGQLVESTYLWKLHDLRNNEAKACDGIHLVPVG-DPIICVKPQQYAMHC 244
           NPLTVPYRAT YGQLVEST+LWKLHDL NNEAKA DGIHLVP G D I+ VK QQYAMHC
Sbjct: 217 NPLTVPYRATTYGQLVESTFLWKLHDLYNNEAKALDGIHLVPAGMDSILHVKSQQYAMHC 276

Query: 245 DAIANEVKLLSEPFRVFEFDFWKRPESHAENEVHVKATDSGRVHAVVSWWTLQLDREGTI 304
           DAI  E+KLLSEPF++FEFDFWKRP+SH E E+ +K+TD GRVHAVVSWW LQLDREGTI
Sbjct: 277 DAITKEIKLLSEPFKIFEFDFWKRPDSHGETELQIKSTDDGRVHAVVSWWVLQLDREGTI 336

Query: 305 FYSTAPKWISPPYNAGAGDWCDHWKQCVWFIPGNGVSISKEEKVHLCASHNDTTFSYHLK 364
           FYSTAP+WIS P +   G+WCDHWKQCVWFIPG G+SI K E++   A H +T+ SY LK
Sbjct: 337 FYSTAPRWISLPIHKSTGNWCDHWKQCVWFIPGKGMSICKGEELLFHALHTETSVSYELK 396

Query: 365 AQIPGGEILKHGVNAENFKLILPPERIAVYGDREWRLAMLTAIKTALQGRAPSACMIADD 424
           +QIP  +  +H +NA++F+L LPPERIA+YGD EWRL+M+ A++ ALQGR    C++ADD
Sbjct: 397 SQIPITDERQHNLNAKDFQLALPPERIAIYGDGEWRLSMVMAMRNALQGRVQPLCVVADD 456

Query: 425 SVFLTLMVACLSKKTLVLSLFPGIREKGTKYLQAVSRVNGICMDSI-------------- 484
           SVFLT+ VA LSK   VLSL PG+ +KG +YL+ V+  N   +D +              
Sbjct: 457 SVFLTICVARLSKTAHVLSLLPGLGDKGAQYLRTVADPNCFSIDRVEILQKGKKCLTMDD 516

Query: 485 ----KVDLLISEPFYYGNDNALPWHNLRFWKERTMLDPVLSDDVLIMPSKGILRACFMSL 544
               KVDLLI EP+Y+GND  LPW NLRFWKER+ LDPVLS +V+IMP KGIL+AC +  
Sbjct: 517 TQQKKVDLLIGEPYYFGNDGMLPWQNLRFWKERSKLDPVLSKEVIIMPFKGILKACAIFC 576

Query: 545 PDLWSSRRRLGTIEGFDHSVTNDTLGACGKSPEGQEGPFLAFYIWQCGEHEELSEIFTLM 604
           PD+W+SRR L  IEGFDH+V N +LGACG  P  ++GP L F+ WQCGE ++LS +FT+M
Sbjct: 577 PDIWNSRRSLSKIEGFDHAVVNTSLGACGDLPAPKDGPCLPFFTWQCGEIKKLSNVFTVM 636

Query: 605 EFDFSKPISPCSGKSQVKVTKAGICHGIVLWIDWLIDSKNSIVISTGPDKRYWKQGVKLL 664
           EFDFSKPISPC GK QV+ T+ G+CHG  LWIDW++DS+NSIVISTGPDKRYWKQGVKL+
Sbjct: 637 EFDFSKPISPCQGKVQVEFTEPGLCHGFALWIDWVLDSENSIVISTGPDKRYWKQGVKLM 696

Query: 665 ADPVAVGPRDSGEGIECCSAAIEASFDPSTGELELRHSF 681
           A PVAVG  +SG    C S  +EASFDPS GEL ++H+F
Sbjct: 697 AKPVAVGFEESGRTDLCSSTLVEASFDPSNGELNVQHTF 734

BLAST of Cucsa.301830 vs. NCBI nr
Match: gi|985446869|ref|XP_015385349.1| (PREDICTED: protein arginine N-methyltransferase 1.6 isoform X1 [Citrus sinensis])

HSP 1 Score: 962.2 bits (2486), Expect = 4.7e-277
Identity = 472/700 (67.43%), Postives = 554/700 (79.14%), Query Frame = 1

Query: 5   STQRLFQLRFDPLTGNSEWVVIEEEAEGVPENSQKPLLATTSYLDMLNDSTRNRAFCEAI 64
           STQR+FQL+ DPLTGNSEWVVIEE  E VPE+SQ+PLLATTSYLDMLNDS RNRA+  AI
Sbjct: 37  STQRMFQLKLDPLTGNSEWVVIEEN-EDVPESSQEPLLATTSYLDMLNDSYRNRAYRLAI 96

Query: 65  DKTISKPCHVLDIGAGTGLLSMMAARAMDSVIG----DCKGMVTACESYLPMVKLMRKVL 124
           DK ++K CHVLDIGAGTGLLSMMAARAM S       + KGMVTACESYLPMVKLM+KVL
Sbjct: 97  DKMVTKSCHVLDIGAGTGLLSMMAARAMGSSDSTTSLNTKGMVTACESYLPMVKLMKKVL 156

Query: 125 RLNGMERKVKVINKRSDEFQFGADFTSRADALVSEILDSELLGEGLIPTLQHAHDKLLVE 184
            +NGM R +KVINKRSDE + G D  SRAD LVSEILDSELLGEGLIPTLQHAHD+LLVE
Sbjct: 157 HVNGMGRNIKVINKRSDELEVGVDIDSRADILVSEILDSELLGEGLIPTLQHAHDRLLVE 216

Query: 185 NPLTVPYRATIYGQLVESTYLWKLHDLRNNEAKACDGIHLVPVG-DPIICVKPQQYAMHC 244
           NPLTVPYRAT YGQLVEST+LWKLHDL NNEAKA DGIHLVP G D I+ VK QQYAMHC
Sbjct: 217 NPLTVPYRATTYGQLVESTFLWKLHDLYNNEAKALDGIHLVPAGMDSILHVKSQQYAMHC 276

Query: 245 DAIANEVKLLSEPFRVFEFDFWKRPESHAENEVHVKATDSGRVHAVVSWWTLQLDREGTI 304
           DAI  E+KLLSEPF++FEFDFWKRP+SH E E+ +K+TD GRVHAVVSWW LQLDREGTI
Sbjct: 277 DAITKEIKLLSEPFKIFEFDFWKRPDSHGETELQIKSTDDGRVHAVVSWWVLQLDREGTI 336

Query: 305 FYSTAPKWISPPYNAGAGDWCDHWKQCVWFIPGNGVSISKEEKVHLCASHNDTTFSYHLK 364
           FYSTAP+WIS P +   G+WCDHWKQCVWFIPG G+SI K E++   A H +T+ SY LK
Sbjct: 337 FYSTAPRWISLPIHKSTGNWCDHWKQCVWFIPGKGMSICKGEELLFHALHTETSVSYELK 396

Query: 365 AQIPGGEILKHGVNAENFKLILPPERIAVYGDREWRLAMLTAIKTALQGRAPSACMIADD 424
           +QIP  +  +H +NA++F+L LPPERIA+YGD EWRL+M+ A++ ALQGR    C++ADD
Sbjct: 397 SQIPITDERQHNLNAKDFQLALPPERIAIYGDGEWRLSMVMAMRNALQGRVQPLCVVADD 456

Query: 425 SVFLTLMVACLSKKTLVLSLFPGIREKGTKYLQAVSRVNGICMDSI-------------- 484
           SVFLT+ VA LSK   VLSL PG+ +KG +YL+ V+  N   +D +              
Sbjct: 457 SVFLTICVARLSKTAHVLSLLPGLGDKGAQYLRTVADPNCFSIDRVEILQKGKKCLTMDD 516

Query: 485 ----KVDLLISEPFYYGNDNALPWHNLRFWKERTMLDPVLSDDVLIMPSKGILRACFMSL 544
               KVDLLI EP+Y+GND  LPW NLRFWKER+ LDPVLS +V+IMP KGIL+AC +  
Sbjct: 517 TQQKKVDLLIGEPYYFGNDGMLPWQNLRFWKERSKLDPVLSKEVIIMPFKGILKACAIFC 576

Query: 545 PDLWSSRRRLGTIEGFDHSVTNDTLGACGKSPEGQEGPFLAFYIWQCGEHEELSEIFTLM 604
           PD+W+SRR L  IEGFDH+V N +LGACG  P  ++GP L F+ WQCGE ++LS +FT+M
Sbjct: 577 PDIWNSRRSLSKIEGFDHAVVNTSLGACGDLPAPKDGPCLPFFTWQCGEIKKLSNVFTVM 636

Query: 605 EFDFSKPISPCSGK-SQVKVTKAGICHGIVLWIDWLIDSKNSIVISTGPDKRYWKQGVKL 664
           EFDFSKPISPC GK  QV+ T+ G+CHG  LWIDW++DS+NSIVISTGPDKRYWKQGVKL
Sbjct: 637 EFDFSKPISPCQGKVQQVEFTEPGLCHGFALWIDWVLDSENSIVISTGPDKRYWKQGVKL 696

Query: 665 LADPVAVGPRDSGEGIECCSAAIEASFDPSTGELELRHSF 681
           +A PVAVG  +SG    C S  +EASFDPS GEL ++H+F
Sbjct: 697 MAKPVAVGFEESGRTDLCSSTLVEASFDPSNGELNVQHTF 735