Cucsa.300610 (gene) Cucumber (Gy14) v1 | Cucurbit Genomics Database (CuGenDB)

Overview


Name	Cucsa.300610
Type	gene
Organism	Cucumis sativus (Cucumber (Gy14) v1)
Description	WRKY protein
Location	scaffold02931 : 163188 .. 166573 (+)

Genome Viewer

Gene Expression

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: five_prime_UTRCDSthree_prime_UTR

Hold the cursor over a type above to highlight its positions in the sequence below.

TCACACACACAACTTTTTTATAAACTCAAACAAACCCTATTTCCCTCTCTCTCTTTCTCTAACACTTTTCAAATCTTCAAAGAAAGAAAATTCCAATTTTGATCAACGAACCGGCCCGCAAGCAGAGGCAGAGCATATCCGGCTCCCATGGAGGACTACAACAACAACAATGATGATGATCAACAACAGATTATAAAGATAGAAGATACTAGCACTATCACTGATGCCGGCGGCGGAGGCGACGGTGGTGGTCGTGGTGGTGGTGGTGGTGGGGTTTTCTCATTTTCCGATAACATAATACCAAATGGATTGTTTGATTTTTGTGATAGTGATAAATGCTCTGTTGGGTTTATGGAGTTGCTTGGTCTTAATTACAATTCTGAATTTACTGAGGCTTTTAATAATCCTCCAGCCACCCCCAATTGTTCGTCCTCCGTCTCCTCCGCCTCTAGCGACGCTCTTAACGACGACGAACCACCACCACCACCACCACCACAACAACAACAACAACACAGCCCCACTAAACAGTTAGTAATTTCTGTTTTCATTCGTTCATTCTTCTTCTTCTTCTTTTCATTTAATTTAAAAAAaGAAAaCTTTTGTTTTCATAATTGTTTTGATCAGATCCATCCTGAAAATGGGGTTTTTTTtAAAATGTAAATGGATTTATTAATTTAATATAAGAAAAAAGGAAACTTGTGGAAGTTATGTACcaagccAagccaTTtGtCTTtACTTTTAAAAAAAAAaGAAAAAAAAAaGAAAGAAAGAAATTAGAAATTAATAAGAAAAATGATAAAATTAGAGTAGTGCTAATTAAATTTTGTGAATAGATTGAAAGGGATAAAAAAGAGGAAGGAGAAGGAGAAGGAGAAGAAAGCAAGATTCGCATTCATGACAAAGAGTGAaGTTGATCATCTAGAAGATGGTTATCGATGGAGAAAGTACGGCCAAAAAGCTGTCAAAAACAGCCCTTTTCCTAGGTATTtCTTTtCTTTtCTTTtCTTtCCCTTTTACCCTTCTCTCCTCTTTCTTTTtCTCTTCTTCTTTtCCTTTTtCTTTTTTCACCACCCACCTTACCTCCCTCCTAACTGCCATCTCCATTCCCTCCTCATCGTTCTTCTACTCAAAATCCCATTTAACTACTTCTTATCTTTCTCATCACCATATCTGTATATTATCTACTCACTTTGTAAGGATTATTAAGTCACAAATGGGTTAAACAAGAAATTAGGAACTTAAGTGCATTTAGGACAAACTACTCTCATCTATAATTAACTACCGATATTAATATTTCAAATTCTACCCCCCTTTCATACTCATCCTGTCTCTCTGACTAATTTGCTCCCAATAAGTATTAATTATTATAACCTATGGAATAAACCAAATTAATGCTCCACATATTGAATGATAGTAGAACGACTCTCCACCTTTAGTTTTAAACTTGTGTGCAATAATGCTTGAGTTTGATTGAAAAGTTGGAGATATTTTTTTtAATTTGTGCTGTTTTTATCTTCTCTATAGAAGTTATTACCGTTGCACGAGTGCAGCATGTAATGTAAAGAAGAGAGTTGAACGATCTTTTGCTGATCCAACTGTAGTGGTGACCACCTATGAAGGCCAACACACTCACCCAAGCCCTATCCTTAGCCGGTCCGCCCTAGCCGTCGCCATTCCACCTCCCTCTTTCATCCCTGGGGCGGGCGGAGAGTGTGTTGGGGGCGTTGTAGCCATGCCATGGCTCAAACCTAGCAACAATGATGCTCATGGTTCGGTTTGTATCCTTACAACTTTTGAGTTATACTTTGAGTATTTTTTttGTTATTGTACATTTAAAATATTCATTGTTTTCGTCTATaCGGTAAAaGAAAAAAaTTATGAGTTAAAAACGCATCTATATTTTGGTACTTTTAAAAGTGATTGTTTCTATCACAACCATTAATTAAGTAGTCATGAATAAGCTAACAAATTAATGATGGAGAGTTTGGGGTTGGGGAAGAGAAATAATTAGTCCAATTGACAACGACGTGTCATCTACTTATTTGTCTTATGGAAAATTAATATAAACATCAAATTATATTTTTtCTTTTGTTTTTTTtGAAAATTAATTAACTCTACAAATACTTCTTCTATCATTTTTAAATCTACATGTTCTGATATTTATTTTTTATTCAAAATCAAGTCAATTTTTTTAAAAAAAAaTGATTAAAAAAAaTAGAATATTTGACAAAATATTTACCATTTATGTAAAAATCAAGTATAATTAATTTTTCTTGTTGGTGGTTTGGTTTTATGAAGGGTAAATAGTTGGTTGATTTTTCTATTTTTGAAAAGATCGGTCTTTAACACGTTAAATTCTGATTTAATTAGATCAAAAATAGGTGAAAAGAAAaGCCAAAAAAaCCCAAGACCAAATGGATTTTTAATTCAAAATCTTTTAGTCCAATCATTTCATTTTCTTtGTTTTtGTTTTTtGAAAATTAAACTTGTTATCTTTCCCTAAaTTTAAAGTAAAATAAGTTTTAAAACTAGTTTTTtAGTTTAAAAAaTTTGACTGATAGTAGATGATAATTCAAGAAATTTAGAGTGAGTGAGTGAAACTTAAAAACAAAAAGAAGACATTTACCTGGTTACTAAATTAGACCATATTATATAGCAAGAAAACTTAAAAGAAAACTTAAAATTCAAACAAAAAAGTTATGAACTATAACAATTGTTATAATTGAAaTAATTtCCTGGTATTTTTTTtCTAATTTTTATATTtCAAACAGATGGTAACACAATTCCAGCAATGTCTCATCAATACTTTCAAAACTCGACCTACATCACTGCCCAAAATGTAGCCGCCAACTATAACCGAAACAACCACATCGGAGCAGCGAACGCGGCCGGAATCCTCCAGGAGAAACGGTTTTGCAACCCAAATTCTAGTTTTCTTGTAGACCATGGCCTGCTTCAAGATGTTGTTCCTCCCCACATGCTGAAACAGGAGTAAAAaTATACTATATATTCTCATATATTACATTATGTAGACATATAGATATAAATATATACTATATATATATATATATTAGTTTAAGTTGGAAAAGAAAACTAAACTGGTGTTTTTGTTTGAATTTTAACCTAGAGAACTGACTTGTGTTGTTCATAGAAGATGTTAGAGGGAACAAACAAAAATTATGAAATTGAAATTTGTTGCTGACAAAGTTTAATTTGGTGATCTTTTCCCTCTCTTGAGGAAAAAAGAGATCGATTTTCCGTATGTTGAAACTTTGGTTACAAGTTCGATGGGTCTTCTAACTTAATTGTATTATTATTCTTTTtATTTTTGTTTATGAGGTTATCAGAAAGAGAAAGATTGATAATTAAGAGCATGTGAGAGAAGA

mRNA sequence

TCACACACACAACTTTTTTATAAACTCAAACAAACCCTATTTCCCTCTCTCTCTTTCTCTAACACTTTTCAAATCTTCAAAGAAAGAAAATTCCAATTTTGATCAACGAACCGGCCCGCAAGCAGAGGCAGAGCATATCCGGCTCCCATGGAGGACTACAACAACAACAATGATGATGATCAACAACAGATTATAAAGATAGAAGATACTAGCACTATCACTGATGCCGGCGGCGGAGGCGACggtggtggtcgtggtggtggtggtggtggGGTTTTCTCATTTTCCGATAACATAATACCAAATGGATTGTTTGATTTTTGTGATAGTGATAAATGCTCTGTTGGGTTTATGGAGTTGCTTGGTCTTAATTACAATTCTGAATTTACTGAGGCTTTTAATAATCCTCCAGCCACCCCCAATTGTTCGTCCTCCGTCTCCTCCGCCTCTAGCGACGCTCTTAACGACGACGAaccaccaccaccaccaccaccacAACAACAACAACAACACAGCCCCACTAAACAATTGAAAGGGATAAAAaagaggaaggagaaggagaaggagaagAAAGCAAGATTCGCATTCATGACAAAGAGTGAAGTTGATCATCTAGAAGATGGTTATCGATGGAGAAAGTACGGCCAAAAAGCTGTCAAAAACAGCCCTTTTCCTAGAAGTTATTACCGTTGCACGAGTGCAGCATGTAATGTAAAGAAGAGAGTTGAACGATCTTTTGCTGATCCAACTGTAGTGGTGACCACCTATGAAGGCCAACACACTCACCCAAGCCCTATCCTTAGCCGGTCCGCCCTAGCCGTCGCCATTCCACCTCCCTCTTTCATCCCTGGGGCGGGCGGAGAGTGTGTTGGGGGCGTTGTAGCCATGCCATGGCTCAAACCTAGCAACAATGATGCTCATGATGGTAACACAATTCCAGCAATGTCTCATCAATACTTTCAAAACTCGACCTACATCACTGCCCAAAATGTAGCCGCCAACTATAACCGAAACAACCACATCGGAGCAGCGAACGCGGCCGGAATCCTCCAGGAGAAACGGTTTTGCAACCCAAATTCTAGTTTTCTTGTAGACCATGGCCTGCTTCAAGATGTTGTTCCTCCCCACATGCTGAAACAGGAGTAAAAATATACTATATATTCTCATATATTACATtatgtagacatatagatataaatatatactatatatatatatatatTAGTTTAAGTTGGAAAAGAAAACTAAACTGGTGTTTTTGTTTGAATTTTAACCTAGAGAACTGACTTGTGTTGTTCATAGAAGATGTTAGAGGGAACAAACAAAAATTATGAAATTGAAATTTGTTGCTGACAAAGTTTAATTTGGTGATCTTTTCCCTCTCTTGAGGAAAAAAGAGATCGATTTTCCGTATGTTGAAACTTTGGTTACAAGTTCGATGGGTCTTCTAACTTAATTGTATTATTATTCTTTTTATTTTTGTTTATGAGGTTATCAGAAAGAGAAAGATTGATAATTAAGAGCATGTGAGAGAAGA

Coding sequence (CDS)

ATGGAGGACTACAACAACAACAATGATGATGATCAACAACAGATTATAAAGATAGAAGATACTAGCACTATCACTGATGCCGGCGGCGGAGGCGACGGTGGTGGTCGTGGTGGTGGTGGTGGTGGGGTTTTCTCATTTTCCGATAACATAATACCAAATGGATTGTTTGATTTTTGTGATAGTGATAAATGCTCTGTTGGGTTTATGGAGTTGCTTGGTCTTAATTACAATTCTGAATTTACTGAGGCTTTTAATAATCCTCCAGCCACCCCCAATTGTTCGTCCTCCGTCTCCTCCGCCTCTAGCGACGCTCTTAACGACGACGAACCACCACCACCACCACCACCACAACAACAACAACAACACAGCCCCACTAAACAATTGAAAGGGATAAAAAAGAGGAAGGAGAAGGAGAAGGAGAAGAAAGCAAGATTCGCATTCATGACAAAGAGTGAaGTTGATCATCTAGAAGATGGTTATCGATGGAGAAAGTACGGCCAAAAAGCTGTCAAAAACAGCCCTTTTCCTAGAAGTTATTACCGTTGCACGAGTGCAGCATGTAATGTAAAGAAGAGAGTTGAACGATCTTTTGCTGATCCAACTGTAGTGGTGACCACCTATGAAGGCCAACACACTCACCCAAGCCCTATCCTTAGCCGGTCCGCCCTAGCCGTCGCCATTCCACCTCCCTCTTTCATCCCTGGGGCGGGCGGAGAGTGTGTTGGGGGCGTTGTAGCCATGCCATGGCTCAAACCTAGCAACAATGATGCTCATGATGGTAACACAATTCCAGCAATGTCTCATCAATACTTTCAAAACTCGACCTACATCACTGCCCAAAATGTAGCCGCCAACTATAACCGAAACAACCACATCGGAGCAGCGAACGCGGCCGGAATCCTCCAGGAGAAACGGTTTTGCAACCCAAATTCTAGTTTTCTTGTAGACCATGGCCTGCTTCAAGATGTTGTTCCTCCCCACATGCTGAAACAGGAGTAA

Protein sequence

MEDYNNNNDDDQQQIIKIEDTSTITDAGGGGDGGGRGGGGGGVFSFSDNIIPNGLFDFCDSDKCSVGFMELLGLNYNSEFTEAFNNPPATPNCSSSVSSASSDALNDDEPPPPPPPQQQQQHSPTKQLKGIKKRKEKEKEKKARFAFMTKSEVDHLEDGYRWRKYGQKAVKNSPFPRSYYRCTSAACNVKKRVERSFADPTVVVTTYEGQHTHPSPILSRSALAVAIPPPSFIPGAGGECVGGVVAMPWLKPSNNDAHDGNTIPAMSHQYFQNSTYITAQNVAANYNRNNHIGAANAAGILQEKRFCNPNSSFLVDHGLLQDVVPPHMLKQE*

Homology

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BLAST of Cucsa.300610 vs. Swiss-Prot
Match: WRK23_ARATH (Probable WRKY transcription factor 23 OS=Arabidopsis thaliana GN=WRKY23 PE=2 SV=1)

HSP 1 Score: 204.9 bits (520), Expect = 1.4e-51
Identity = 129/259 (49.81%), Postives = 157/259 (60.62%), Query Frame = 1


Query: 86  NPPATPNCSSSVSSASSDALNDDEPPPPPPPQQ----QQQHSPTKQLKGIKKRKEKEKEK 145
           NPPATPN SSS+SSASS+ALN+++P      ++    QQ+ S TK+    KK  +K +++
Sbjct: 101 NPPATPN-SSSISSASSEALNEEKPKTEDNEEEGGEDQQEKSHTKKQLKAKKNNQK-RQR 160

Query: 146 KARFAFMTKSEVDHLEDGYRWRKYGQKAVKNSPFPRSYYRCTSAACNVKKRVERSFADPT 205
           +AR AFMTKSEVDHLEDGYRWRKYGQKAVKNSPFPRSYYRCT+A+CNVKKRVERSF DP+
Sbjct: 161 EARVAFMTKSEVDHLEDGYRWRKYGQKAVKNSPFPRSYYRCTTASCNVKKRVERSFRDPS 220

Query: 206 VVVTTYEGQHTHPSPILSRSALAVAIPPPSFIPGAGGECVGGVVAMPWLKPSNNDAHDGN 265
            VVTTYEGQHTH SP+ SR          S   GA      G    P          DG+
Sbjct: 221 TVVTTYEGQHTHISPLTSRPISTGGFFGSS---GAASSLGNGCFGFP---------IDGS 280

Query: 266 TIPAMSHQYFQNSTYITAQNV--------AANYNRNNHIGAANAAGILQEKRFCNPNSSF 325
           T+  +S Q+ Q   Y   Q            N   N+H   AN  G   +      +   
Sbjct: 281 TL--ISPQFQQLVQYHHQQQQQELMSCFGGVNEYLNSH---ANEYG---DDNRVKKSRVL 337

Query: 326 LVDHGLLQDVVPPHMLKQE 333
           + D+GLLQDVVP HMLK+E
Sbjct: 341 VKDNGLLQDVVPSHMLKEE 337

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BLAST of Cucsa.300610 vs. Swiss-Prot
Match: WRK68_ARATH (Probable WRKY transcription factor 68 OS=Arabidopsis thaliana GN=WRKY68 PE=2 SV=1)

HSP 1 Score: 184.9 bits (468), Expect = 1.5e-45
Identity = 110/281 (39.15%), Postives = 162/281 (57.65%), Query Frame = 1


Query: 55  LFDFCD--SDKCSVGFMELLGLNYNSEFTEAFNNPPATPNCSSSVSSASSDALNDDEPPP 114
           L DF D  +++  VGFM+LLG++ ++       + P TPN      S+SS+A+N D+   
Sbjct: 22  LSDFHDLSAERYPVGFMDLLGVHRHTPTHTPLMHFPTTPN------SSSSEAVNGDDEEE 81

Query: 115 PPPPQQQQQHSPTKQLKGIKKRKEKEKEKKARFAFMTKSEVDHLEDGYRWRKYGQKAVKN 174
                ++QQH   K+ K  K  +++ K+K  + +F+T+SEV HL+DGY+WRKYGQK VK+
Sbjct: 82  EDG--EEQQHKTKKRFKFTKMSRKQTKKKVPKVSFITRSEVLHLDDGYKWRKYGQKPVKD 141

Query: 175 SPFPRSYYRCTSAACNVKKRVERSFADPTVVVTTYEGQHTHPSPILSRSALAVAIPPPSF 234
           SPFPR+YYRCT+  C+VKKRVERSF+DP+ V+TTYEGQHTHP P+L        I P   
Sbjct: 142 SPFPRNYYRCTTTWCDVKKRVERSFSDPSSVITTYEGQHTHPRPLL--------IMPKEG 201

Query: 235 IPGAGGECVGGVVAMPWLKPSNNDAHDGNTIPAMSHQYFQNSTYITAQNVAANY-NRNNH 294
              + G      + +P L P   D ++       S      + YI  Q    N+ + ++H
Sbjct: 202 SSPSNGSASRAHIGLPTLPPQLLDYNNQQQQAPSSF----GTEYINRQEKGINHDDDDDH 261

Query: 295 IGAANAAGILQEKRFCNPNSSFLVDHGLLQDVVPPHMLKQE 333
           +   +    L +       +  + DHGLLQDVVP H++K+E
Sbjct: 262 VVKKSRTRDLLD------GAGLVKDHGLLQDVVPSHIIKEE 276

[Close]

BLAST of Cucsa.300610 vs. Swiss-Prot
Match: WRK48_ARATH (Probable WRKY transcription factor 48 OS=Arabidopsis thaliana GN=WRKY48 PE=2 SV=1)

HSP 1 Score: 178.7 bits (452), Expect = 1.0e-43
Identity = 106/272 (38.97%), Postives = 146/272 (53.68%), Query Frame = 1


Query: 78  SEFTEAFNNPPATPNCSSSVSSASSDALNDDEPPPPPPPQQQQQHSPTKQLKGIK----- 137
           +E +E  N  P +PN S+SVSS+S++A ND+        + Q++    ++ KG K     
Sbjct: 138 AESSEVVNTTPTSPN-STSVSSSSNEAANDNNSGKEVTVKDQEEGDQQQEQKGTKPQLKA 197

Query: 138 KRKEKEKEKKARFAFMTKSEVDHLEDGYRWRKYGQKAVKNSPFPRSYYRCTSAACNVKKR 197
           K+K ++K ++ARFAF+TKS++D+L+DGYRWRKYGQKAVKNSP+PRSYYRCT+  C VKKR
Sbjct: 198 KKKNQKKAREARFAFLTKSDIDNLDDGYRWRKYGQKAVKNSPYPRSYYRCTTVGCGVKKR 257

Query: 198 VERSFADPTVVVTTYEGQHTHPSPILSRSALAVAIPPPSFIPGAGGECVGGVVAMPWLKP 257
           VERS  DP++V+TTYEGQHTHP P+  R  +                   G++  P L  
Sbjct: 258 VERSSDDPSIVMTTYEGQHTHPFPMTPRGHI-------------------GMLTSPILD- 317

Query: 258 SNNDAHDGNTIPAMSHQYFQNSTYITAQNVAANYNRNNHIGAANAAGILQEKRFCNP--- 317
                H   T  + S    Q    +T  +   N   NN +   N      +  F NP   
Sbjct: 318 -----HGATTASSSSFSIPQPRYLLTQHHQPYNMYNNNSLSMINRRS--SDGTFVNPGPS 377

Query: 318 ----------------NSSFLVDHGLLQDVVP 326
                            SS + DHGLLQD++P
Sbjct: 378 SSFPGFGYDMSQASTSTSSSIRDHGLLQDILP 381

[Close]

BLAST of Cucsa.300610 vs. Swiss-Prot
Match: WRK71_ARATH (Probable WRKY transcription factor 71 OS=Arabidopsis thaliana GN=WRKY71 PE=2 SV=1)

HSP 1 Score: 164.1 bits (414), Expect = 2.7e-39
Identity = 105/271 (38.75%), Postives = 142/271 (52.40%), Query Frame = 1


Query: 76  YNSEF---TEAFNNPPATPNCSSSVSSASSDALNDDEPPPPPPPQQQQQHS--------- 135
           Y+S F   + + NNP      +S V S+SS   N+ EP      +  Q            
Sbjct: 45  YSSPFQSVSPSVNNPYLNLTSNSPVVSSSS---NEGEPKENTNDKSDQMEDNEGDLHGVG 104

Query: 136 -PTKQLKGIKKRKEKEKEKKARFAFMTKSEVDHLEDGYRWRKYGQKAVKNSPFPRSYYRC 195
             +KQL    K+K ++KE++ R AFMTKSE+DHLEDGYRWRKYGQKAVKNSP+PRSYYRC
Sbjct: 105 ESSKQLTKQGKKKGEKKEREVRVAFMTKSEIDHLEDGYRWRKYGQKAVKNSPYPRSYYRC 164

Query: 196 TSAACNVKKRVERSFADPTVVVTTYEGQHTHPSPILSRSALAVAIPPPSFIPGAGGECVG 255
           T+  CNVKKRVERSF DP++V+TTYEG+H HP P    S L   +     +   GG   G
Sbjct: 165 TTQKCNVKKRVERSFQDPSIVITTYEGKHNHPIP----STLRGTVAAEHLLVHRGG---G 224

Query: 256 GVVAMPWLKPSNNDAHDGNTIPAMSHQY-FQNSTYITAQNVAANYNRNNHIGAANAAGIL 315
           G                     ++ H +   +  ++  ++  ANY     +   +  G  
Sbjct: 225 G---------------------SLLHSFPRHHQDFLMMKHSPANYQSVGSLSYEHGHG-- 281

Query: 316 QEKRFCNPNSSFLVDHGLLQDVVPPHMLKQE 333
               +   N+  +VD+GLLQD+VP    K E
Sbjct: 285 -TSSYNFNNNQPVVDYGLLQDIVPSMFSKNE 281

[Close]

BLAST of Cucsa.300610 vs. Swiss-Prot
Match: WRKY8_ARATH (Probable WRKY transcription factor 8 OS=Arabidopsis thaliana GN=WRKY8 PE=1 SV=1)

HSP 1 Score: 156.4 bits (394), Expect = 5.5e-37
Identity = 89/195 (45.64%), Postives = 117/195 (60.00%), Query Frame = 1


Query: 47  SDNIIPNGLFDF------CDSDKCSVGFMELLGL----NYNS--EFTEAFNNPPATPNCS 106
           S N+ P G F+         SD  +    +  GL    NYNS  +  E   +  AT    
Sbjct: 55  SKNLSPQGAFELGFELSPSSSDFFNPSLDQENGLYNAYNYNSSQKSHEVVGDGCATIKSE 114

Query: 107 SSVSSASSDALNDDEPPPPPPPQQQQQH------SPTKQLKGIKKRKEKEKEKKARFAFM 166
             VS++ S +  D  P       ++++          +  K +K +K++EK+K+ R +FM
Sbjct: 115 VRVSASPSSSEADHHPGEDSGKIRKKREVRDGGEDDQRSQKVVKTKKKEEKKKEPRVSFM 174

Query: 167 TKSEVDHLEDGYRWRKYGQKAVKNSPFPRSYYRCTSAACNVKKRVERSFADPTVVVTTYE 224
           TK+EVDHLEDGYRWRKYGQKAVKNSP+PRSYYRCT+  CNVKKRVERS+ DPTVV+TTYE
Sbjct: 175 TKTEVDHLEDGYRWRKYGQKAVKNSPYPRSYYRCTTQKCNVKKRVERSYQDPTVVITTYE 234

[Close]

BLAST of Cucsa.300610 vs. TrEMBL
Match: E7CEY9_CUCSA (WRKY protein OS=Cucumis sativus GN=WRKY55 PE=2 SV=1)

HSP 1 Score: 547.4 bits (1409), Expect = 1.2e-152
Identity = 262/264 (99.24%), Postives = 263/264 (99.62%), Query Frame = 1


Query: 69  MELLGLNYNSEFTEAFNNPPATPNCSSSVSSASSDALNDDEPPPPPPPQQQQQHSPTKQL 128
           MELLGLNYNSEFTEAFNNPPATPNCSSSVSSASSDALNDDEPPPPPP QQQQQHSPTKQL
Sbjct: 1   MELLGLNYNSEFTEAFNNPPATPNCSSSVSSASSDALNDDEPPPPPPQQQQQQHSPTKQL 60

Query: 129 KGIKKRKEKEKEKKARFAFMTKSEVDHLEDGYRWRKYGQKAVKNSPFPRSYYRCTSAACN 188
           KGIKKRKEKEKEKKARFAFMTKSEVDHLEDGYRWRKYGQKAVKNSPFPRSYYRCTSAACN
Sbjct: 61  KGIKKRKEKEKEKKARFAFMTKSEVDHLEDGYRWRKYGQKAVKNSPFPRSYYRCTSAACN 120

Query: 189 VKKRVERSFADPTVVVTTYEGQHTHPSPILSRSALAVAIPPPSFIPGAGGECVGGVVAMP 248
           VKKRVERSFADPTVVVTTYEGQHTHPSPILSRSALAVAIPPPSFIPGAGGECVGGVVAMP
Sbjct: 121 VKKRVERSFADPTVVVTTYEGQHTHPSPILSRSALAVAIPPPSFIPGAGGECVGGVVAMP 180

Query: 249 WLKPSNNDAHDGNTIPAMSHQYFQNSTYITAQNVAANYNRNNHIGAANAAGILQEKRFCN 308
           WLKPSNNDAHDGNT+PAMSHQYFQNSTYITAQNVAANYNRNNHIGAANAAGILQEKRFCN
Sbjct: 181 WLKPSNNDAHDGNTVPAMSHQYFQNSTYITAQNVAANYNRNNHIGAANAAGILQEKRFCN 240

Query: 309 PNSSFLVDHGLLQDVVPPHMLKQE 333
           PNSSFLVDHGLLQDVVPPHMLKQE
Sbjct: 241 PNSSFLVDHGLLQDVVPPHMLKQE 264

[Close]

BLAST of Cucsa.300610 vs. TrEMBL
Match: A0A151SMU3_CAJCA (Putative WRKY transcription factor 23 OS=Cajanus cajan GN=KK1_002397 PE=4 SV=1)

HSP 1 Score: 248.1 bits (632), Expect = 1.6e-62
Identity = 150/321 (46.73%), Postives = 191/321 (59.50%), Query Frame = 1


Query: 46  FSDNIIP-NGLFDFCDSDKCSVGFMELLGLNYNSEF------------------------ 105
           ++ N+ P +  FDF + +K S+GFMELLG+   S                          
Sbjct: 22  YNSNVYPFSSAFDFPEVEKNSLGFMELLGVQDYSPLLELPQLSTLSVPHHSTVKLASDNC 81

Query: 106 TEAFNNPPATPNCSSSVSSASSDALNDD-----EPPPPPPPQQQQQHSPTKQLKGIKKRK 165
           +E  N  PATPN SSS+SSASSDA+ND+     +       +++ Q    KQLK   K+ 
Sbjct: 82  SEVLNQLPATPN-SSSISSASSDAVNDEQNKTLDQAEEDEEEEEGQQKTKKQLK--PKKT 141

Query: 166 EKEKEKKARFAFMTKSEVDHLEDGYRWRKYGQKAVKNSPFPRSYYRCTSAACNVKKRVER 225
            ++++++ RFAFMTKSEVDHLEDGYRWRKYGQKAVKNSPFPRSYYRCTS +CNVKKRVER
Sbjct: 142 NQKRQREPRFAFMTKSEVDHLEDGYRWRKYGQKAVKNSPFPRSYYRCTSVSCNVKKRVER 201

Query: 226 SFADPTVVVTTYEGQHTHPSPILSRSALAVAIPPPSFIPGAGGECVGGVVAMPWLKPSNN 285
           SF DP+VVVTTYEGQHTHPSP++ RS ++           AG     G V  P       
Sbjct: 202 SFTDPSVVVTTYEGQHTHPSPVMPRSGVS-----------AGYATNFGSVFPP------- 261

Query: 286 DAHDGNTIPAMSHQYF---QNSTYITAQNVAANYNRNNHIGAANAAGILQEKRFCNP-NS 333
               GN +    HQ+    Q   + T  +++  YN +    +A  AG  QE+R CNP  +
Sbjct: 262 ----GNYLSQYQHQHHNHQQQQLFNTLSSLSFPYNDS----SAKNAGFTQERRLCNPGTN 313

[Close]

BLAST of Cucsa.300610 vs. TrEMBL
Match: V7BJB6_PHAVU (Uncharacterized protein OS=Phaseolus vulgaris GN=PHAVU_007G212900g PE=4 SV=1)

HSP 1 Score: 247.3 bits (630), Expect = 2.7e-62
Identity = 150/333 (45.05%), Postives = 191/333 (57.36%), Query Frame = 1


Query: 39  GGGGVFSFSDNIIP-NGLFDFCDSDKCSVGFMELLGLNYNS------------------- 98
           G   +  ++ N+ P +  FDF + +K S+GFMELLG+   S                   
Sbjct: 15  GSSSIPCYNSNVYPFSNAFDFSEVEKSSLGFMELLGVQDYSPLLELPQLSTVSVPHHSTV 74

Query: 99  --------EFTEAFNNPPATPNCSSSVSSASSDALNDD--------EPPPPPPPQQQQQH 158
                   E +E  N+ PATPN SSS+SSASSDA+ND+        E       +++ Q 
Sbjct: 75  KVPSDNGKESSEVLNHQPATPN-SSSISSASSDAVNDEQKMTLDQVEEDDDEEEEEEGQQ 134

Query: 159 SPTKQLKGIKKRKEKEKEKKARFAFMTKSEVDHLEDGYRWRKYGQKAVKNSPFPRSYYRC 218
              KQLK   K+  ++++++ RFAFMTKSEVDHLEDGYRWRKYGQKAVKNSPFPRSYYRC
Sbjct: 135 KTKKQLK--PKKTNQKRQREPRFAFMTKSEVDHLEDGYRWRKYGQKAVKNSPFPRSYYRC 194

Query: 219 TSAACNVKKRVERSFADPTVVVTTYEGQHTHPSPILSRSALAV--AIPPPSFIPGAGGEC 278
           TS +CNVKKRVERSF DP+VVVTTYEGQHTHPSP++ RS ++   A    S  P AG   
Sbjct: 195 TSVSCNVKKRVERSFTDPSVVVTTYEGQHTHPSPVMPRSGVSAGYATNLASVFPQAGN-- 254

Query: 279 VGGVVAMPWLKPSNNDAHDGNTIPAMSHQYFQNSTYITAQNVAANYNRNNHIGAANAAGI 333
                   +L       H             Q   + T  ++   YN ++   +   AG 
Sbjct: 255 --------YLSQYQQQHH------------HQQLIFSTLSSLGFPYNDSS---SPKNAGF 314

[Close]

BLAST of Cucsa.300610 vs. TrEMBL
Match: G8Z9V5_HEVBR (WRKY1 OS=Hevea brasiliensis GN=WRKY1 PE=2 SV=1)

HSP 1 Score: 246.5 bits (628), Expect = 4.5e-62
Identity = 151/321 (47.04%), Postives = 189/321 (58.88%), Query Frame = 1


Query: 42  GVFSFSDNIIPN----GLFDFCDSDKCSVGFMELLG-----------LNYNSEFTEAFNN 101
           G  +F DN   +    G+FDFC+ DK S+GFMELLG           L   S    A +N
Sbjct: 14  GSSTFPDNTQSSYPFQGVFDFCEGDKSSLGFMELLGMQDFSASVFDMLQVPSVVQPAASN 73

Query: 102 P-------------PATPNCSSSVSSASSDALNDDEPPPPPPPQQQQQHSPTKQLKGIKK 161
           P             PATPN SSS+SSASSDALND EP      ++++Q    K+LK   K
Sbjct: 74  PVATKMESPEVLNQPATPN-SSSISSASSDALND-EPVKVADNEEEEQQKTRKELK--PK 133

Query: 162 RKEKEKEKKARFAFMTKSEVDHLEDGYRWRKYGQKAVKNSPFPRSYYRCTSAACNVKKRV 221
           +  ++++++ RFAFMTKSEVDHLEDGYRWRKYGQKAVKNSPFPRSYYRCTSA+CNVKKRV
Sbjct: 134 KTNQKRQREPRFAFMTKSEVDHLEDGYRWRKYGQKAVKNSPFPRSYYRCTSASCNVKKRV 193

Query: 222 ERSFADPTVVVTTYEGQHTHPSPILSRSALAVAIPPPSFIPGAGGECVGGVVAMPWLKPS 281
           ERSF+DP++VVTTYEGQHTHPS +++R +   A     F   A         AMP  +  
Sbjct: 194 ERSFSDPSIVVTTYEGQHTHPSAVMARPSFTGAASESGFSTSA--------FAMPMQR-- 253

Query: 282 NNDAHDGNTIPAMSHQYFQNSTYITA-QNVAANYNRNNHIGAANAAGILQEKRFC-NPNS 333
                       +SH   Q   ++ +   +   Y  N +      A  L E+RFC +P S
Sbjct: 254 -----------RLSHFQQQQQPFLNSFTALGFGYKGNTN------ATFLHERRFCTSPGS 303

[Close]

BLAST of Cucsa.300610 vs. TrEMBL
Match: A0A0A7E9T3_GOSAI (WRKY65 OS=Gossypium aridum PE=2 SV=1)

HSP 1 Score: 243.8 bits (621), Expect = 2.9e-61
Identity = 148/314 (47.13%), Postives = 181/314 (57.64%), Query Frame = 1


Query: 45  SFSDNIIPNGLFDF-CDS-DKCSVGFMELLG------------------------LNYNS 104
           SFSD      +FD+ C+  +   +GFMELLG                        LN  S
Sbjct: 17  SFSDYFPLQSIFDWSCEGGENMPLGFMELLGVQDLKPPLFDMGQQQAPSAVVNQALNPCS 76

Query: 105 EFTEAFNNPPATPNCSSSVSSASSDALNDDEPPPPPPPQQQQQHSPTKQLKGIKKRKEKE 164
              E FN  PATPN SSS+SSASS+A+ND EP      Q++ Q    KQLK   K+  ++
Sbjct: 77  TKNERFNYQPATPN-SSSISSASSEAVND-EPIQVDDDQEEDQQKTKKQLK--PKKTNQK 136

Query: 165 KEKKARFAFMTKSEVDHLEDGYRWRKYGQKAVKNSPFPRSYYRCTSAACNVKKRVERSFA 224
           ++++ RFAFMTKSEVDHLEDGYRWRKYGQKAVKNS FPRSYYRCT+ +CNVKKRVERSF 
Sbjct: 137 RQREPRFAFMTKSEVDHLEDGYRWRKYGQKAVKNSSFPRSYYRCTTTSCNVKKRVERSFT 196

Query: 225 DPTVVVTTYEGQHTHPSPILSRSALAVAIPPPSFIPGAGGECVGGVVAMPWLKPSNNDAH 284
           DP++V+TTYEGQHTHPSPI+ RS+L                  GG  + P+         
Sbjct: 197 DPSMVITTYEGQHTHPSPIMPRSSLG-----------------GGAGSAPYHCRQQRQPF 256

Query: 285 DGNTIPAMSHQYFQNSTYITAQNVAANYNRNNHIGAANAAGILQEKRFCNPNSSFLVDHG 333
             NT+P +                  N+  NN  G+ N      E+RFCNP SS L DHG
Sbjct: 257 -VNTLPPL------------------NFGHNN--GSINGGNFHHERRFCNPGSSLLKDHG 288

[Close]

BLAST of Cucsa.300610 vs. TAIR10
Match: AT2G47260.1 (AT2G47260.1 WRKY DNA-binding protein 23)

HSP 1 Score: 204.9 bits (520), Expect = 7.6e-53
Identity = 129/259 (49.81%), Postives = 157/259 (60.62%), Query Frame = 1


Query: 86  NPPATPNCSSSVSSASSDALNDDEPPPPPPPQQ----QQQHSPTKQLKGIKKRKEKEKEK 145
           NPPATPN SSS+SSASS+ALN+++P      ++    QQ+ S TK+    KK  +K +++
Sbjct: 101 NPPATPN-SSSISSASSEALNEEKPKTEDNEEEGGEDQQEKSHTKKQLKAKKNNQK-RQR 160

Query: 146 KARFAFMTKSEVDHLEDGYRWRKYGQKAVKNSPFPRSYYRCTSAACNVKKRVERSFADPT 205
           +AR AFMTKSEVDHLEDGYRWRKYGQKAVKNSPFPRSYYRCT+A+CNVKKRVERSF DP+
Sbjct: 161 EARVAFMTKSEVDHLEDGYRWRKYGQKAVKNSPFPRSYYRCTTASCNVKKRVERSFRDPS 220

Query: 206 VVVTTYEGQHTHPSPILSRSALAVAIPPPSFIPGAGGECVGGVVAMPWLKPSNNDAHDGN 265
            VVTTYEGQHTH SP+ SR          S   GA      G    P          DG+
Sbjct: 221 TVVTTYEGQHTHISPLTSRPISTGGFFGSS---GAASSLGNGCFGFP---------IDGS 280

Query: 266 TIPAMSHQYFQNSTYITAQNV--------AANYNRNNHIGAANAAGILQEKRFCNPNSSF 325
           T+  +S Q+ Q   Y   Q            N   N+H   AN  G   +      +   
Sbjct: 281 TL--ISPQFQQLVQYHHQQQQQELMSCFGGVNEYLNSH---ANEYG---DDNRVKKSRVL 337

Query: 326 LVDHGLLQDVVPPHMLKQE 333
           + D+GLLQDVVP HMLK+E
Sbjct: 341 VKDNGLLQDVVPSHMLKEE 337

[Close]

BLAST of Cucsa.300610 vs. TAIR10
Match: AT3G62340.1 (AT3G62340.1 WRKY family transcription factor)

HSP 1 Score: 184.9 bits (468), Expect = 8.2e-47
Identity = 110/281 (39.15%), Postives = 162/281 (57.65%), Query Frame = 1


Query: 55  LFDFCD--SDKCSVGFMELLGLNYNSEFTEAFNNPPATPNCSSSVSSASSDALNDDEPPP 114
           L DF D  +++  VGFM+LLG++ ++       + P TPN      S+SS+A+N D+   
Sbjct: 22  LSDFHDLSAERYPVGFMDLLGVHRHTPTHTPLMHFPTTPN------SSSSEAVNGDDEEE 81

Query: 115 PPPPQQQQQHSPTKQLKGIKKRKEKEKEKKARFAFMTKSEVDHLEDGYRWRKYGQKAVKN 174
                ++QQH   K+ K  K  +++ K+K  + +F+T+SEV HL+DGY+WRKYGQK VK+
Sbjct: 82  EDG--EEQQHKTKKRFKFTKMSRKQTKKKVPKVSFITRSEVLHLDDGYKWRKYGQKPVKD 141

Query: 175 SPFPRSYYRCTSAACNVKKRVERSFADPTVVVTTYEGQHTHPSPILSRSALAVAIPPPSF 234
           SPFPR+YYRCT+  C+VKKRVERSF+DP+ V+TTYEGQHTHP P+L        I P   
Sbjct: 142 SPFPRNYYRCTTTWCDVKKRVERSFSDPSSVITTYEGQHTHPRPLL--------IMPKEG 201

Query: 235 IPGAGGECVGGVVAMPWLKPSNNDAHDGNTIPAMSHQYFQNSTYITAQNVAANY-NRNNH 294
              + G      + +P L P   D ++       S      + YI  Q    N+ + ++H
Sbjct: 202 SSPSNGSASRAHIGLPTLPPQLLDYNNQQQQAPSSF----GTEYINRQEKGINHDDDDDH 261

Query: 295 IGAANAAGILQEKRFCNPNSSFLVDHGLLQDVVPPHMLKQE 333
           +   +    L +       +  + DHGLLQDVVP H++K+E
Sbjct: 262 VVKKSRTRDLLD------GAGLVKDHGLLQDVVPSHIIKEE 276

[Close]

BLAST of Cucsa.300610 vs. TAIR10
Match: AT5G49520.1 (AT5G49520.1 WRKY DNA-binding protein 48)

HSP 1 Score: 178.7 bits (452), Expect = 5.9e-45
Identity = 106/272 (38.97%), Postives = 146/272 (53.68%), Query Frame = 1


Query: 78  SEFTEAFNNPPATPNCSSSVSSASSDALNDDEPPPPPPPQQQQQHSPTKQLKGIK----- 137
           +E +E  N  P +PN S+SVSS+S++A ND+        + Q++    ++ KG K     
Sbjct: 138 AESSEVVNTTPTSPN-STSVSSSSNEAANDNNSGKEVTVKDQEEGDQQQEQKGTKPQLKA 197

Query: 138 KRKEKEKEKKARFAFMTKSEVDHLEDGYRWRKYGQKAVKNSPFPRSYYRCTSAACNVKKR 197
           K+K ++K ++ARFAF+TKS++D+L+DGYRWRKYGQKAVKNSP+PRSYYRCT+  C VKKR
Sbjct: 198 KKKNQKKAREARFAFLTKSDIDNLDDGYRWRKYGQKAVKNSPYPRSYYRCTTVGCGVKKR 257

Query: 198 VERSFADPTVVVTTYEGQHTHPSPILSRSALAVAIPPPSFIPGAGGECVGGVVAMPWLKP 257
           VERS  DP++V+TTYEGQHTHP P+  R  +                   G++  P L  
Sbjct: 258 VERSSDDPSIVMTTYEGQHTHPFPMTPRGHI-------------------GMLTSPILD- 317

Query: 258 SNNDAHDGNTIPAMSHQYFQNSTYITAQNVAANYNRNNHIGAANAAGILQEKRFCNP--- 317
                H   T  + S    Q    +T  +   N   NN +   N      +  F NP   
Sbjct: 318 -----HGATTASSSSFSIPQPRYLLTQHHQPYNMYNNNSLSMINRRS--SDGTFVNPGPS 377

Query: 318 ----------------NSSFLVDHGLLQDVVP 326
                            SS + DHGLLQD++P
Sbjct: 378 SSFPGFGYDMSQASTSTSSSIRDHGLLQDILP 381

[Close]

BLAST of Cucsa.300610 vs. TAIR10
Match: AT1G29860.1 (AT1G29860.1 WRKY DNA-binding protein 71)

HSP 1 Score: 164.1 bits (414), Expect = 1.5e-40
Identity = 105/271 (38.75%), Postives = 142/271 (52.40%), Query Frame = 1


Query: 76  YNSEF---TEAFNNPPATPNCSSSVSSASSDALNDDEPPPPPPPQQQQQHS--------- 135
           Y+S F   + + NNP      +S V S+SS   N+ EP      +  Q            
Sbjct: 45  YSSPFQSVSPSVNNPYLNLTSNSPVVSSSS---NEGEPKENTNDKSDQMEDNEGDLHGVG 104

Query: 136 -PTKQLKGIKKRKEKEKEKKARFAFMTKSEVDHLEDGYRWRKYGQKAVKNSPFPRSYYRC 195
             +KQL    K+K ++KE++ R AFMTKSE+DHLEDGYRWRKYGQKAVKNSP+PRSYYRC
Sbjct: 105 ESSKQLTKQGKKKGEKKEREVRVAFMTKSEIDHLEDGYRWRKYGQKAVKNSPYPRSYYRC 164

Query: 196 TSAACNVKKRVERSFADPTVVVTTYEGQHTHPSPILSRSALAVAIPPPSFIPGAGGECVG 255
           T+  CNVKKRVERSF DP++V+TTYEG+H HP P    S L   +     +   GG   G
Sbjct: 165 TTQKCNVKKRVERSFQDPSIVITTYEGKHNHPIP----STLRGTVAAEHLLVHRGG---G 224

Query: 256 GVVAMPWLKPSNNDAHDGNTIPAMSHQY-FQNSTYITAQNVAANYNRNNHIGAANAAGIL 315
           G                     ++ H +   +  ++  ++  ANY     +   +  G  
Sbjct: 225 G---------------------SLLHSFPRHHQDFLMMKHSPANYQSVGSLSYEHGHG-- 281

Query: 316 QEKRFCNPNSSFLVDHGLLQDVVPPHMLKQE 333
               +   N+  +VD+GLLQD+VP    K E
Sbjct: 285 -TSSYNFNNNQPVVDYGLLQDIVPSMFSKNE 281

[Close]

BLAST of Cucsa.300610 vs. TAIR10
Match: AT5G46350.1 (AT5G46350.1 WRKY DNA-binding protein 8)

HSP 1 Score: 156.4 bits (394), Expect = 3.1e-38
Identity = 89/195 (45.64%), Postives = 117/195 (60.00%), Query Frame = 1


Query: 47  SDNIIPNGLFDF------CDSDKCSVGFMELLGL----NYNS--EFTEAFNNPPATPNCS 106
           S N+ P G F+         SD  +    +  GL    NYNS  +  E   +  AT    
Sbjct: 55  SKNLSPQGAFELGFELSPSSSDFFNPSLDQENGLYNAYNYNSSQKSHEVVGDGCATIKSE 114

Query: 107 SSVSSASSDALNDDEPPPPPPPQQQQQH------SPTKQLKGIKKRKEKEKEKKARFAFM 166
             VS++ S +  D  P       ++++          +  K +K +K++EK+K+ R +FM
Sbjct: 115 VRVSASPSSSEADHHPGEDSGKIRKKREVRDGGEDDQRSQKVVKTKKKEEKKKEPRVSFM 174

Query: 167 TKSEVDHLEDGYRWRKYGQKAVKNSPFPRSYYRCTSAACNVKKRVERSFADPTVVVTTYE 224
           TK+EVDHLEDGYRWRKYGQKAVKNSP+PRSYYRCT+  CNVKKRVERS+ DPTVV+TTYE
Sbjct: 175 TKTEVDHLEDGYRWRKYGQKAVKNSPYPRSYYRCTTQKCNVKKRVERSYQDPTVVITTYE 234

[Close]

BLAST of Cucsa.300610 vs. NCBI nr
Match: gi|778681548|ref|XP_011651538.1| (PREDICTED: probable WRKY transcription factor 68 isoform X1 [Cucumis sativus])

HSP 1 Score: 685.6 bits (1768), Expect = 4.2e-194
Identity = 327/332 (98.49%), Postives = 328/332 (98.80%), Query Frame = 1


Query: 1   MEDYNNNNDDDQQQIIKIEDTSTITDAGGGGDGGGRGGGGGGVFSFSDNIIPNGLFDFCD 60
           MEDYNNNNDDDQQQIIKIEDTSTITD GGGGDGGGRGGG GGVFSFSDNIIPNGLFDFCD
Sbjct: 1   MEDYNNNNDDDQQQIIKIEDTSTITDGGGGGDGGGRGGGDGGVFSFSDNIIPNGLFDFCD 60

Query: 61  SDKCSVGFMELLGLNYNSEFTEAFNNPPATPNCSSSVSSASSDALNDDEPPPPPPPQQQQ 120
            DKCSVGFMELLGLNYNSEFTEAFNNPPATPNCSSSVSSASSDALNDDEPPPPPP QQQQ
Sbjct: 61  GDKCSVGFMELLGLNYNSEFTEAFNNPPATPNCSSSVSSASSDALNDDEPPPPPPQQQQQ 120

Query: 121 QHSPTKQLKGIKKRKEKEKEKKARFAFMTKSEVDHLEDGYRWRKYGQKAVKNSPFPRSYY 180
           QHSPTKQLKGIKKRKEKEKEKKARFAFMTKSEVDHLEDGYRWRKYGQKAVKNSPFPRSYY
Sbjct: 121 QHSPTKQLKGIKKRKEKEKEKKARFAFMTKSEVDHLEDGYRWRKYGQKAVKNSPFPRSYY 180

Query: 181 RCTSAACNVKKRVERSFADPTVVVTTYEGQHTHPSPILSRSALAVAIPPPSFIPGAGGEC 240
           RCTSAACNVKKRVERSFADPTVVVTTYEGQHTHPSPILSRSALAVAIPPPSFIPGAGGEC
Sbjct: 181 RCTSAACNVKKRVERSFADPTVVVTTYEGQHTHPSPILSRSALAVAIPPPSFIPGAGGEC 240

Query: 241 VGGVVAMPWLKPSNNDAHDGNTIPAMSHQYFQNSTYITAQNVAANYNRNNHIGAANAAGI 300
           VGGVVAMPWLKPSNNDAHDGNT+PAMSHQYFQNSTYITAQNVAANYNRNNHIGAANAAGI
Sbjct: 241 VGGVVAMPWLKPSNNDAHDGNTVPAMSHQYFQNSTYITAQNVAANYNRNNHIGAANAAGI 300

Query: 301 LQEKRFCNPNSSFLVDHGLLQDVVPPHMLKQE 333
           LQEKRFCNPNSSFLVDHGLLQDVVPPHMLKQE
Sbjct: 301 LQEKRFCNPNSSFLVDHGLLQDVVPPHMLKQE 332

[Close]

BLAST of Cucsa.300610 vs. NCBI nr
Match: gi|315613854|gb|ADU52532.1| (WRKY protein [Cucumis sativus])

HSP 1 Score: 547.4 bits (1409), Expect = 1.8e-152
Identity = 262/264 (99.24%), Postives = 263/264 (99.62%), Query Frame = 1


Query: 69  MELLGLNYNSEFTEAFNNPPATPNCSSSVSSASSDALNDDEPPPPPPPQQQQQHSPTKQL 128
           MELLGLNYNSEFTEAFNNPPATPNCSSSVSSASSDALNDDEPPPPPP QQQQQHSPTKQL
Sbjct: 1   MELLGLNYNSEFTEAFNNPPATPNCSSSVSSASSDALNDDEPPPPPPQQQQQQHSPTKQL 60

Query: 129 KGIKKRKEKEKEKKARFAFMTKSEVDHLEDGYRWRKYGQKAVKNSPFPRSYYRCTSAACN 188
           KGIKKRKEKEKEKKARFAFMTKSEVDHLEDGYRWRKYGQKAVKNSPFPRSYYRCTSAACN
Sbjct: 61  KGIKKRKEKEKEKKARFAFMTKSEVDHLEDGYRWRKYGQKAVKNSPFPRSYYRCTSAACN 120

Query: 189 VKKRVERSFADPTVVVTTYEGQHTHPSPILSRSALAVAIPPPSFIPGAGGECVGGVVAMP 248
           VKKRVERSFADPTVVVTTYEGQHTHPSPILSRSALAVAIPPPSFIPGAGGECVGGVVAMP
Sbjct: 121 VKKRVERSFADPTVVVTTYEGQHTHPSPILSRSALAVAIPPPSFIPGAGGECVGGVVAMP 180

Query: 249 WLKPSNNDAHDGNTIPAMSHQYFQNSTYITAQNVAANYNRNNHIGAANAAGILQEKRFCN 308
           WLKPSNNDAHDGNT+PAMSHQYFQNSTYITAQNVAANYNRNNHIGAANAAGILQEKRFCN
Sbjct: 181 WLKPSNNDAHDGNTVPAMSHQYFQNSTYITAQNVAANYNRNNHIGAANAAGILQEKRFCN 240

Query: 309 PNSSFLVDHGLLQDVVPPHMLKQE 333
           PNSSFLVDHGLLQDVVPPHMLKQE
Sbjct: 241 PNSSFLVDHGLLQDVVPPHMLKQE 264

[Close]

BLAST of Cucsa.300610 vs. NCBI nr
Match: gi|778681554|ref|XP_011651540.1| (PREDICTED: probable WRKY transcription factor 68 isoform X2 [Cucumis sativus])

HSP 1 Score: 533.9 bits (1374), Expect = 2.0e-148
Identity = 254/258 (98.45%), Postives = 254/258 (98.45%), Query Frame = 1


Query: 1   MEDYNNNNDDDQQQIIKIEDTSTITDAGGGGDGGGRGGGGGGVFSFSDNIIPNGLFDFCD 60
           MEDYNNNNDDDQQQIIKIEDTSTITD GGGGDGGGRGGG GGVFSFSDNIIPNGLFDFCD
Sbjct: 1   MEDYNNNNDDDQQQIIKIEDTSTITDGGGGGDGGGRGGGDGGVFSFSDNIIPNGLFDFCD 60

Query: 61  SDKCSVGFMELLGLNYNSEFTEAFNNPPATPNCSSSVSSASSDALNDDEPPPPPPPQQQQ 120
            DKCSVGFMELLGLNYNSEFTEAFNNPPATPNCSSSVSSASSDALNDDEPPPPPP QQQQ
Sbjct: 61  GDKCSVGFMELLGLNYNSEFTEAFNNPPATPNCSSSVSSASSDALNDDEPPPPPPQQQQQ 120

Query: 121 QHSPTKQLKGIKKRKEKEKEKKARFAFMTKSEVDHLEDGYRWRKYGQKAVKNSPFPRSYY 180
           QHSPTKQLKGIKKRKEKEKEKKARFAFMTKSEVDHLEDGYRWRKYGQKAVKNSPFPRSYY
Sbjct: 121 QHSPTKQLKGIKKRKEKEKEKKARFAFMTKSEVDHLEDGYRWRKYGQKAVKNSPFPRSYY 180

Query: 181 RCTSAACNVKKRVERSFADPTVVVTTYEGQHTHPSPILSRSALAVAIPPPSFIPGAGGEC 240
           RCTSAACNVKKRVERSFADPTVVVTTYEGQHTHPSPILSRSALAVAIPPPSFIPGAGGEC
Sbjct: 181 RCTSAACNVKKRVERSFADPTVVVTTYEGQHTHPSPILSRSALAVAIPPPSFIPGAGGEC 240

Query: 241 VGGVVAMPWLKPSNNDAH 259
           VGGVVAMPWLKPSNNDAH
Sbjct: 241 VGGVVAMPWLKPSNNDAH 258

[Close]

BLAST of Cucsa.300610 vs. NCBI nr
Match: gi|1012344968|gb|KYP56160.1| (putative WRKY transcription factor 23 [Cajanus cajan])

HSP 1 Score: 248.1 bits (632), Expect = 2.2e-62
Identity = 150/321 (46.73%), Postives = 191/321 (59.50%), Query Frame = 1


Query: 46  FSDNIIP-NGLFDFCDSDKCSVGFMELLGLNYNSEF------------------------ 105
           ++ N+ P +  FDF + +K S+GFMELLG+   S                          
Sbjct: 22  YNSNVYPFSSAFDFPEVEKNSLGFMELLGVQDYSPLLELPQLSTLSVPHHSTVKLASDNC 81

Query: 106 TEAFNNPPATPNCSSSVSSASSDALNDD-----EPPPPPPPQQQQQHSPTKQLKGIKKRK 165
           +E  N  PATPN SSS+SSASSDA+ND+     +       +++ Q    KQLK   K+ 
Sbjct: 82  SEVLNQLPATPN-SSSISSASSDAVNDEQNKTLDQAEEDEEEEEGQQKTKKQLK--PKKT 141

Query: 166 EKEKEKKARFAFMTKSEVDHLEDGYRWRKYGQKAVKNSPFPRSYYRCTSAACNVKKRVER 225
            ++++++ RFAFMTKSEVDHLEDGYRWRKYGQKAVKNSPFPRSYYRCTS +CNVKKRVER
Sbjct: 142 NQKRQREPRFAFMTKSEVDHLEDGYRWRKYGQKAVKNSPFPRSYYRCTSVSCNVKKRVER 201

Query: 226 SFADPTVVVTTYEGQHTHPSPILSRSALAVAIPPPSFIPGAGGECVGGVVAMPWLKPSNN 285
           SF DP+VVVTTYEGQHTHPSP++ RS ++           AG     G V  P       
Sbjct: 202 SFTDPSVVVTTYEGQHTHPSPVMPRSGVS-----------AGYATNFGSVFPP------- 261

Query: 286 DAHDGNTIPAMSHQYF---QNSTYITAQNVAANYNRNNHIGAANAAGILQEKRFCNP-NS 333
               GN +    HQ+    Q   + T  +++  YN +    +A  AG  QE+R CNP  +
Sbjct: 262 ----GNYLSQYQHQHHNHQQQQLFNTLSSLSFPYNDS----SAKNAGFTQERRLCNPGTN 313

[Close]

BLAST of Cucsa.300610 vs. NCBI nr
Match: gi|1009147973|ref|XP_015891697.1| (PREDICTED: probable WRKY transcription factor 23 [Ziziphus jujuba])

HSP 1 Score: 248.1 bits (632), Expect = 2.2e-62
Identity = 157/367 (42.78%), Postives = 208/367 (56.68%), Query Frame = 1


Query: 11  DQQQIIKIEDTSTITDAGGGGDGGGRGGGGGGVFSFSDNIIPNGLFDFCDSDKCS-VGFM 70
           ++++IIK+E+            G    G GGG ++ S      G+FD C+++K S +GFM
Sbjct: 2   EKREIIKMEENYNSI-------GSLTFGEGGGGYTLS------GMFDLCEAEKSSSLGFM 61

Query: 71  ELLGLNYNS------------------------------------EFTEAFNNPPATPNC 130
           ELLG+   S                                    + +EA N P ATPN 
Sbjct: 62  ELLGVQDISPCLFDFPHTLTATSSSSSTLLVPSAPTIIPTPAARKDSSEALNQP-ATPN- 121

Query: 131 SSSVSSASSDALNDDEPPPPPPPQQQ-----QQHSPTKQLKGIKKRKEKEKEKKARFAFM 190
           SSS+SSASS+A+N+++       Q +     + H  TK+    KK  +K ++++ RFAFM
Sbjct: 122 SSSISSASSEAINEEQTKTDNQDQDEGEEEHEDHQKTKKQLKAKKTNQK-RQREPRFAFM 181

Query: 191 TKSEVDHLEDGYRWRKYGQKAVKNSPFPRSYYRCTSAACNVKKRVERSFADPTVVVTTYE 250
           TKSEVDHLEDGYRWRKYGQKAVKNSPFPRSYYRCTS +CNVKKRVERSF+DP+VVVTTYE
Sbjct: 182 TKSEVDHLEDGYRWRKYGQKAVKNSPFPRSYYRCTSTSCNVKKRVERSFSDPSVVVTTYE 241

Query: 251 GQHTHPSPILSRSALAVAIPPPSFIPGAGGECVGGVVAMPWLKPSNNDAHDGNTIPAMSH 310
           GQHTHPSP+++R + A A+ PP     A   C     AMP   P+            +SH
Sbjct: 242 GQHTHPSPVMARPSHAAAVLPP-----ASTACATTNFAMPMPTPT-----------TISH 301

Query: 311 QYFQNSTYITAQNVAANYNRNNHIGAANAA--GILQEKRFCN-PNSSFLVDHGLLQDVVP 333
            +     +    N+  ++N   + G+ N+    IL E+R CN    S L DHGLLQD++P
Sbjct: 302 YHHHQQNHPFLDNLTPSFN-FGYQGSTNSKTNTILHERRLCNLAGPSLLADHGLLQDIIP 335

The following BLAST results are available for this feature:

BLAST of Cucsa.300610 vs. Swiss-Prot


Match Name	E-value	Identity	Description
WRK23_ARATH	1.4e-51	49.81	Probable WRKY transcription factor 23 OS=Arabidopsis thaliana GN=WRKY23 PE=2 SV=...	[more]
WRK68_ARATH	1.5e-45	39.15	Probable WRKY transcription factor 68 OS=Arabidopsis thaliana GN=WRKY68 PE=2 SV=...	[more]
WRK48_ARATH	1.0e-43	38.97	Probable WRKY transcription factor 48 OS=Arabidopsis thaliana GN=WRKY48 PE=2 SV=...	[more]
WRK71_ARATH	2.7e-39	38.75	Probable WRKY transcription factor 71 OS=Arabidopsis thaliana GN=WRKY71 PE=2 SV=...	[more]
WRKY8_ARATH	5.5e-37	45.64	Probable WRKY transcription factor 8 OS=Arabidopsis thaliana GN=WRKY8 PE=1 SV=1	[more]

BLAST of Cucsa.300610 vs. TrEMBL


Match Name	E-value	Identity	Description
E7CEY9_CUCSA	1.2e-152	99.24	WRKY protein OS=Cucumis sativus GN=WRKY55 PE=2 SV=1	[more]
A0A151SMU3_CAJCA	1.6e-62	46.73	Putative WRKY transcription factor 23 OS=Cajanus cajan GN=KK1_002397 PE=4 SV=1	[more]
V7BJB6_PHAVU	2.7e-62	45.05	Uncharacterized protein OS=Phaseolus vulgaris GN=PHAVU_007G212900g PE=4 SV=1	[more]
G8Z9V5_HEVBR	4.5e-62	47.04	WRKY1 OS=Hevea brasiliensis GN=WRKY1 PE=2 SV=1	[more]
A0A0A7E9T3_GOSAI	2.9e-61	47.13	WRKY65 OS=Gossypium aridum PE=2 SV=1	[more]

BLAST of Cucsa.300610 vs. TAIR10


Match Name	E-value	Identity	Description
AT2G47260.1	7.6e-53	49.81	WRKY DNA-binding protein 23	[more]
AT3G62340.1	8.2e-47	39.15	WRKY family transcription factor	[more]
AT5G49520.1	5.9e-45	38.97	WRKY DNA-binding protein 48	[more]
AT1G29860.1	1.5e-40	38.75	WRKY DNA-binding protein 71	[more]
AT5G46350.1	3.1e-38	45.64	WRKY DNA-binding protein 8	[more]

BLAST of Cucsa.300610 vs. NCBI nr


Match Name	E-value	Identity	Description
gi\|778681548\|ref\|XP_011651538.1\|	4.2e-194	98.49	PREDICTED: probable WRKY transcription factor 68 isoform X1 [Cucumis sativus]	[more]
gi\|315613854\|gb\|ADU52532.1\|	1.8e-152	99.24	WRKY protein [Cucumis sativus]	[more]
gi\|778681554\|ref\|XP_011651540.1\|	2.0e-148	98.45	PREDICTED: probable WRKY transcription factor 68 isoform X2 [Cucumis sativus]	[more]
gi\|1012344968\|gb\|KYP56160.1\|	2.2e-62	46.73	putative WRKY transcription factor 23 [Cajanus cajan]	[more]
gi\|1009147973\|ref\|XP_015891697.1\|	2.2e-62	42.78	PREDICTED: probable WRKY transcription factor 23 [Ziziphus jujuba]	[more]

Annotated Terms

The following terms have been associated with this gene:

Vocabulary: **INTERPRO**
Term	Definition
IPR003657	WRKY_dom
IPR003657	WRKY_dom
IPR003657	WRKY_dom
IPR003657	WRKY_dom

Vocabulary: **Molecular Function**
Term	Definition
GO:0003700	transcription factor activity, sequence-specific DNA binding
GO:0043565	sequence-specific DNA binding
GO:0003700	transcription factor activity, sequence-specific DNA binding
GO:0043565	sequence-specific DNA binding
GO:0003700	transcription factor activity, sequence-specific DNA binding
GO:0043565	sequence-specific DNA binding
GO:0003700	transcription factor activity, sequence-specific DNA binding
GO:0043565	sequence-specific DNA binding

Vocabulary: **Biological Process**
Term	Definition
GO:0006355	regulation of transcription, DNA-templated
GO:0006355	regulation of transcription, DNA-templated
GO:0006355	regulation of transcription, DNA-templated
GO:0006355	regulation of transcription, DNA-templated

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0006355	regulation of transcription, DNA-templated
cellular_component	GO:0005667	transcription factor complex
molecular_function	GO:0043565	sequence-specific DNA binding
molecular_function	GO:0003700	transcription factor activity, sequence-specific DNA binding

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Cucsa.300610.1	Cucsa.300610.1	mRNA
Cucsa.300610.2	Cucsa.300610.2	mRNA
Cucsa.300610.3	Cucsa.300610.3	mRNA
Cucsa.300610.4	Cucsa.300610.4	mRNA

InterPro

Analysis Name: InterPro Annotations of cucumber (Gy14)
Date Performed: 2017-01-17


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR003657	WRKY domain	GENE3D	G3DSA:2.20.25.80		coord: 69..118 score: 2.5
IPR003657	WRKY domain	PFAM	PF03106	WRKY	coord: 69..116 score: 1.5
IPR003657	WRKY domain	SMART	SM00774	WRKY_cls	coord: 69..117 score: 1.4
IPR003657	WRKY domain	PROFILE	PS50811	WRKY	coord: 53..118 score: 19
IPR003657	WRKY domain	unknown	SSF118290	WRKY DNA-binding domain	coord: 69..118 score: 1.57
None	No IPR available	unknown	Coil	Coil	coord: 1..21 scor
None	No IPR available	PANTHER	PTHR31221	FAMILY NOT NAMED	coord: 80..234 score: 5.6
None	No IPR available	PANTHER	PTHR31221:SF13	WRKY TRANSCRIPTION FACTOR 48-RELATED	coord: 80..234 score: 5.6

Synteny

The following gene(s) are orthologous to this gene:


Gene	Orthologue	Organism	Block
Cucsa.300610	Cla017345	Watermelon (97103) v1	cgywmB546
Cucsa.300610	MELO3C012932	Melon (DHL92) v3.5.1	cgymeB494
Cucsa.300610	ClCG10G011570	Watermelon (Charleston Gray)	cgywcgB518
Cucsa.300610	CSPI03G25020	Wild cucumber (PI 183967)	cgycpiB451
Cucsa.300610	CmaCh14G009400	Cucurbita maxima (Rimu)	cgycmaB0828
Cucsa.300610	CmaCh01G002790	Cucurbita maxima (Rimu)	cgycmaB0829
Cucsa.300610	CmoCh01G002870	Cucurbita moschata (Rifu)	cgycmoB0822
Cucsa.300610	Lsi03G011240	Bottle gourd (USVL1VR-Ls)	cgylsiB485
Cucsa.300610	Cp4.1LG02g16250	Cucurbita pepo (Zucchini)	cgycpeB0785
Cucsa.300610	MELO3C012932.2	Melon (DHL92) v3.6.1	cgymedB493
Cucsa.300610	CsaV3_3G026600	Cucumber (Chinese Long) v3	cgycucB464
Cucsa.300610	Cla97C10G194390	Watermelon (97103) v2	cgywmbB519
Cucsa.300610	Bhi11G001082	Wax gourd	cgywgoB600
Cucsa.300610	Carg07371	Silver-seed gourd	carcgyB0865
Cucsa.300610	Carg11165	Silver-seed gourd	carcgyB0934

The following gene(s) are paralogous to this gene:

None

The following block(s) are covering this gene:


Gene	Organism	Block
Cucsa.300610	Wax gourd	cgywgoB601
Cucsa.300610	Cucumber (Chinese Long) v2	cgycuB428