Cucsa.275720 (gene) Cucumber (Gy14) v1

BLAST of Cucsa.275720 vs. Swiss-Prot
Match: PP440_ARATH (Pentatricopeptide repeat-containing protein At5g61400 OS=Arabidopsis thaliana GN=At5g61400 PE=2 SV=1)

HSP 1 Score: 537.3 bits (1383), Expect = 2.1e-151
Identity = 282/617 (45.71%), Postives = 393/617 (63.70%), Query Frame = 1

Query: 6   SLSSLTPDSLITTVLNCRSPWKALEFFNAAPEKNI------QLYSAIIHVLVGSKLLSHA 65
           S SS +  SL   +L CRS  +A + F  +    +      Q +SA+IHVL G+   + A
Sbjct: 35  SASSFSSSSLAEAILKCRSAEEAFKLFETSSRSRVSKSNDLQSFSAVIHVLTGAHKYTLA 94

Query: 66  RYLLNDLVQNLVKSHKPYHACQLAFSELSRLKSSKFTPNVYGELIIVLCKMELVEEALSM 125
           R L+  L++ L +  +P +     F+ L  ++S KF+  V+  LI+   +M L EEAL +
Sbjct: 95  RCLIKSLIERLKRHSEPSNMSHRLFNALEDIQSPKFSIGVFSLLIMEFLEMGLFEEALWV 154

Query: 126 YHKVGAALTIQACNVLLYVLVKTGRFELLWRIYEEMISNGLSPSVITFGTLIDGCCRQGD 185
             ++  +   +AC  +L  LV+  RF+ +W  Y+ MIS GL P V  +  L   C +QG 
Sbjct: 155 SREMKCSPDSKACLSILNGLVRRRRFDSVWVDYQLMISRGLVPDVHIYFVLFQCCFKQGL 214

Query: 186 LLRAQEMFDEMRVKGIVPTVIVYTILIRGLCSDNKIEEAESMHRAMREVGVYPNVYTYNT 245
             + +++ DEM   GI P V +YTI I  LC DNK+EEAE M   M++ GV PN+YTY+ 
Sbjct: 215 YSKKEKLLDEMTSLGIKPNVYIYTIYILDLCRDNKMEEAEKMFELMKKHGVLPNLYTYSA 274

Query: 246 LMDGYCKLANAKQALRLYQDMLGEGLVPDVVTFGILIDGLCKFGEMKAARNLFVNMIKFS 305
           ++DGYCK  N +QA  LY+++L   L+P+VV FG L+DG CK  E+  AR+LFV+M+KF 
Sbjct: 275 MIDGYCKTGNVRQAYGLYKEILVAELLPNVVVFGTLVDGFCKARELVTARSLFVHMVKFG 334

Query: 306 VTPNIAVYNSLIDAYCKVGDVSEAMALFLELERFEVSPDVFTYSILIRGLCSVSRTEEAG 365
           V PN+ VYN LI  +CK G++ EA+ L  E+E   +SPDVFTY+ILI GLC   +  EA 
Sbjct: 335 VDPNLYVYNCLIHGHCKSGNMLEAVGLLSEMESLNLSPDVFTYTILINGLCIEDQVAEAN 394

Query: 366 NIFEKMTKEGILANSVTYNSLIDGCCKEGKMDKALEICSQMTENGVEPNVITFSTLIDGY 425
            +F+KM  E I  +S TYNSLI G CKE  M++AL++CS+MT +GVEPN+ITFSTLIDGY
Sbjct: 395 RLFQKMKNERIFPSSATYNSLIHGYCKEYNMEQALDLCSEMTASGVEPNIITFSTLIDGY 454

Query: 426 CKIRNLQAAMGIYSEMVIKSLSPDVVTYTAMIDGHCKYGSMKEALKLYSDMLDNGITPNC 485
           C +R+++AAMG+Y EM IK + PDVVTYTA+ID H K  +MKEAL+LYSDML+ GI PN 
Sbjct: 455 CNVRDIKAAMGLYFEMTIKGIVPDVVTYTALIDAHFKEANMKEALRLYSDMLEAGIHPND 514

Query: 486 YTISCLLDGLCKDGKISDALELFTEKIEFQTPRCNVDAGGSKPSLTNHVAYTALIHGLCQ 545
           +T +CL+DG  K+G++S A++ + E                + S  NHV +T LI GLCQ
Sbjct: 515 HTFACLVDGFWKEGRLSVAIDFYQE-------------NNQQRSCWNHVGFTCLIEGLCQ 574

Query: 546 DGQFSKAVKLFSDMRRYGLQPDEVIYVVMLRGLFQVKYI--LMMLHADMLKFGVIPNSAV 605
           +G   +A + FSDMR  G+ PD   YV ML+G  Q K I   MML  DM+K G++PN  V
Sbjct: 575 NGYILRASRFFSDMRSCGITPDICSYVSMLKGHLQEKRITDTMMLQCDMIKTGILPNLLV 634

Query: 606 HVILCECYQESGFLKSA 615
           + +L   YQ +G++KSA
Sbjct: 635 NQLLARFYQANGYVKSA 638

BLAST of Cucsa.275720 vs. Swiss-Prot
Match: PP143_ARATH (Putative pentatricopeptide repeat-containing protein At2g02150 OS=Arabidopsis thaliana GN=At2g02150 PE=3 SV=1)

HSP 1 Score: 296.6 bits (758), Expect = 6.3e-79
Identity = 174/566 (30.74%), Postives = 296/566 (52.30%), Query Frame = 1

Query: 25  PWKALEFFNAAPEKN-----IQLYSAIIHVLVGSKLLSHARYLLNDLVQNLVKSHKPYHA 84
           P  A +FF  +  +N     ++ Y  + H+L  +++     Y  N +++ +V S      
Sbjct: 122 PKLAFKFFKWSMTRNGFKHSVESYCIVAHILFCARMY----YDANSVLKEMVLSKADCDV 181

Query: 85  CQLAFSELSRLKSSKFTPNVYGELIIVLCKMELVEEALSMYHKVGAALTI---QACNVLL 144
             + +S  +          V+  L  VL  + ++EEA+  + K+         ++CN LL
Sbjct: 182 FDVLWSTRNVCVPGF---GVFDALFSVLIDLGMLEEAIQCFSKMKRFRVFPKTRSCNGLL 241

Query: 145 YVLVKTGRFELLWRIYEEMISNGLSPSVITFGTLIDGCCRQGDLLRAQEMFDEMRVKGIV 204
           +   K G+ + + R +++MI  G  P+V T+  +ID  C++GD+  A+ +F+EM+ +G+V
Sbjct: 242 HRFAKLGKTDDVKRFFKDMIGAGARPTVFTYNIMIDCMCKEGDVEAARGLFEEMKFRGLV 301

Query: 205 PTVIVYTILIRGLCSDNKIEEAESMHRAMREVGVYPNVYTYNTLMDGYCKLANAKQALRL 264
           P  + Y  +I G     ++++       M+++   P+V TYN L++ +CK       L  
Sbjct: 302 PDTVTYNSMIDGFGKVGRLDDTVCFFEEMKDMCCEPDVITYNALINCFCKFGKLPIGLEF 361

Query: 265 YQDMLGEGLVPDVVTFGILIDGLCKFGEMKAARNLFVNMIKFSVTPNIAVYNSLIDAYCK 324
           Y++M G GL P+VV++  L+D  CK G M+ A   +V+M +  + PN   Y SLIDA CK
Sbjct: 362 YREMKGNGLKPNVVSYSTLVDAFCKEGMMQQAIKFYVDMRRVGLVPNEYTYTSLIDANCK 421

Query: 325 VGDVSEAMALFLELERFEVSPDVFTYSILIRGLCSVSRTEEAGNIFEKMTKEGILANSVT 384
           +G++S+A  L  E+ +  V  +V TY+ LI GLC   R +EA  +F KM   G++ N  +
Sbjct: 422 IGNLSDAFRLGNEMLQVGVEWNVVTYTALIDGLCDAERMKEAEELFGKMDTAGVIPNLAS 481

Query: 385 YNSLIDGCCKEGKMDKALEICSQMTENGVEPNVITFSTLIDGYCKIRNLQAAMGIYSEMV 444
           YN+LI G  K   MD+ALE+ +++   G++P+++ + T I G C +  ++AA  + +EM 
Sbjct: 482 YNALIHGFVKAKNMDRALELLNELKGRGIKPDLLLYGTFIWGLCSLEKIEAAKVVMNEMK 541

Query: 445 IKSLSPDVVTYTAMIDGHCKYGSMKEALKLYSDMLDNGITPNCYTISCLLDGLCKDGKIS 504
              +  + + YT ++D + K G+  E L L  +M +  I     T   L+DGLCK+  +S
Sbjct: 542 ECGIKANSLIYTTLMDAYFKSGNPTEGLHLLDEMKELDIEVTVVTFCVLIDGLCKNKLVS 601

Query: 505 DALELFTEKIEFQTPRCNVDAGGSKPSLTNHVAYTALIHGLCQDGQFSKAVKLFSDMRRY 564
            A++ F         R + D G       N   +TA+I GLC+D Q   A  LF  M + 
Sbjct: 602 KAVDYFN--------RISNDFG----LQANAAIFTAMIDGLCKDNQVEAATTLFEQMVQK 661

Query: 565 GLQPDEVIYVVMLRGLFQVKYILMML 583
           GL PD   Y  ++ G F+   +L  L
Sbjct: 662 GLVPDRTAYTSLMDGNFKQGNVLEAL 668

HSP 2 Score: 258.1 bits (658), Expect = 2.5e-67
Identity = 159/480 (33.12%), Postives = 244/480 (50.83%), Query Frame = 1

Query: 100 YGELIIVLCKMELVEEALSMYHKV---GAALTIQACNVLLYVLVKTGRFELLWRIYEEMI 159
           Y  +I  +CK   VE A  ++ ++   G        N ++    K GR +     +EEM 
Sbjct: 265 YNIMIDCMCKEGDVEAARGLFEEMKFRGLVPDTVTYNSMIDGFGKVGRLDDTVCFFEEMK 324

Query: 160 SNGLSPSVITFGTLIDGCCRQGDLLRAQEMFDEMRVKGIVPTVIVYTILIRGLCSDNKIE 219
                P VIT+  LI+  C+ G L    E + EM+  G+ P V+ Y+ L+   C +  ++
Sbjct: 325 DMCCEPDVITYNALINCFCKFGKLPIGLEFYREMKGNGLKPNVVSYSTLVDAFCKEGMMQ 384

Query: 220 EAESMHRAMREVGVYPNVYTYNTLMDGYCKLANAKQALRLYQDMLGEGLVPDVVTFGILI 279
           +A   +  MR VG+ PN YTY +L+D  CK+ N   A RL  +ML  G+  +VVT+  LI
Sbjct: 385 QAIKFYVDMRRVGLVPNEYTYTSLIDANCKIGNLSDAFRLGNEMLQVGVEWNVVTYTALI 444

Query: 280 DGLCKFGEMKAARNLFVNMIKFSVTPNIAVYNSLIDAYCKVGDVSEAMALFLELERFEVS 339
           DGLC    MK A  LF  M    V PN+A YN+LI  + K  ++  A+ L  EL+   + 
Sbjct: 445 DGLCDAERMKEAEELFGKMDTAGVIPNLASYNALIHGFVKAKNMDRALELLNELKGRGIK 504

Query: 340 PDVFTYSILIRGLCSVSRTEEAGNIFEKMTKEGILANSVTYNSLIDGCCKEGKMDKALEI 399
           PD+  Y   I GLCS+ + E A  +  +M + GI ANS+ Y +L+D   K G   + L +
Sbjct: 505 PDLLLYGTFIWGLCSLEKIEAAKVVMNEMKECGIKANSLIYTTLMDAYFKSGNPTEGLHL 564

Query: 400 CSQMTENGVEPNVITFSTLIDGYCKIRNLQAAMGIYSEMVIK-SLSPDVVTYTAMIDGHC 459
             +M E  +E  V+TF  LIDG CK + +  A+  ++ +     L  +   +TAMIDG C
Sbjct: 565 LDEMKELDIEVTVVTFCVLIDGLCKNKLVSKAVDYFNRISNDFGLQANAAIFTAMIDGLC 624

Query: 460 KYGSMKEALKLYSDMLDNGITPNCYTISCLLDGLCKDGKISDALELFTEKIEFQTPRCNV 519
           K   ++ A  L+  M+  G+ P+    + L+DG  K G + +AL L  +  E        
Sbjct: 625 KDNQVEAATTLFEQMVQKGLVPDRTAYTSLMDGNFKQGNVLEALALRDKMAEI------- 684

Query: 520 DAGGSKPSLTNHVAYTALIHGLCQDGQFSKAVKLFSDMRRYGLQPDEVIYVVMLRGLFQV 576
              G K  L   +AYT+L+ GL    Q  KA     +M   G+ PDEV+ + +L+  +++
Sbjct: 685 ---GMKLDL---LAYTSLVWGLSHCNQLQKARSFLEEMIGEGIHPDEVLCISVLKKHYEL 731

HSP 3 Score: 226.5 bits (576), Expect = 8.1e-58
Identity = 150/536 (27.99%), Postives = 257/536 (47.95%), Query Frame = 1

Query: 84  FSELSRLKSSKFTPNVYGEL--IIVLCKMELVEEALSMYHKVGAALTIQACNVLLYVLVK 143
           FS++ R +    T +  G L     L K + V+         GA  T+   N+++  + K
Sbjct: 215 FSKMKRFRVFPKTRSCNGLLHRFAKLGKTDDVKRFFKDMIGAGARPTVFTYNIMIDCMCK 274

Query: 144 TGRFELLWRIYEEMISNGLSPSVITFGTLIDGCCRQGDLLRAQEMFDEMRVKGIVPTVIV 203
            G  E    ++EEM   GL P  +T+ ++IDG  + G L      F+EM+     P VI 
Sbjct: 275 EGDVEAARGLFEEMKFRGLVPDTVTYNSMIDGFGKVGRLDDTVCFFEEMKDMCCEPDVIT 334

Query: 204 YTILIRGLCSDNKIEEAESMHRAMREVGVYPNVYTYNTLMDGYCKLANAKQALRLYQDML 263
           Y  LI   C   K+      +R M+  G+ PNV +Y+TL+D +CK    +QA++ Y DM 
Sbjct: 335 YNALINCFCKFGKLPIGLEFYREMKGNGLKPNVVSYSTLVDAFCKEGMMQQAIKFYVDMR 394

Query: 264 GEGLVPDVVTFGILIDGLCKFGEMKAARNLFVNMIKFSVTPNIAVYNSLIDAYCKVGDVS 323
             GLVP+  T+  LID  CK G +  A  L   M++  V  N+  Y +LID  C    + 
Sbjct: 395 RVGLVPNEYTYTSLIDANCKIGNLSDAFRLGNEMLQVGVEWNVVTYTALIDGLCDAERMK 454

Query: 324 EAMALFLELERFEVSPDVFTYSILIRGLCSVSRTEEAGNIFEKMTKEGILANSVTYNSLI 383
           EA  LF +++   V P++ +Y+ LI G       + A  +  ++   GI  + + Y + I
Sbjct: 455 EAEELFGKMDTAGVIPNLASYNALIHGFVKAKNMDRALELLNELKGRGIKPDLLLYGTFI 514

Query: 384 DGCCKEGKMDKALEICSQMTENGVEPNVITFSTLIDGYCKIRNLQAAMGIYSEMVIKSLS 443
            G C   K++ A  + ++M E G++ N + ++TL+D Y K  N    + +  EM    + 
Sbjct: 515 WGLCSLEKIEAAKVVMNEMKECGIKANSLIYTTLMDAYFKSGNPTEGLHLLDEMKELDIE 574

Query: 444 PDVVTYTAMIDGHCKYGSMKEALKLYSDML-DNGITPNCYTISCLLDGLCKDGKISDALE 503
             VVT+  +IDG CK   + +A+  ++ +  D G+  N    + ++DGLCKD ++  A  
Sbjct: 575 VTVVTFCVLIDGLCKNKLVSKAVDYFNRISNDFGLQANAAIFTAMIDGLCKDNQVEAATT 634

Query: 504 LFTEKIEFQTPRCNVDAGGSKPSLTNHVAYTALIHGLCQDGQFSKAVKLFSDMRRYGLQP 563
           LF + ++             K  + +  AYT+L+ G  + G   +A+ L   M   G++ 
Sbjct: 635 LFEQMVQ-------------KGLVPDRTAYTSLMDGNFKQGNVLEALALRDKMAEIGMKL 694

Query: 564 DEVIYVVMLRGLFQVKYI--LMMLHADMLKFGVIPNSAVHVILCECYQESGFLKSA 615
           D + Y  ++ GL     +        +M+  G+ P+  + + + + + E G +  A
Sbjct: 695 DLLAYTSLVWGLSHCNQLQKARSFLEEMIGEGIHPDEVLCISVLKKHYELGCIDEA 737

HSP 4 Score: 209.5 bits (532), Expect = 1.0e-52
Identity = 134/489 (27.40%), Postives = 244/489 (49.90%), Query Frame = 1

Query: 30  EFFNAAPEKNIQLYSAIIHVLVGSKLLSHARYLLNDL-VQNLVKSHKPYHACQLAFSELS 89
           +   A     +  Y+ +I  +     +  AR L  ++  + LV     Y++    F ++ 
Sbjct: 252 DMIGAGARPTVFTYNIMIDCMCKEGDVEAARGLFEEMKFRGLVPDTVTYNSMIDGFGKVG 311

Query: 90  RL----------KSSKFTPNV--YGELIIVLCKMELVEEALSMYHKV---GAALTIQACN 149
           RL          K     P+V  Y  LI   CK   +   L  Y ++   G    + + +
Sbjct: 312 RLDDTVCFFEEMKDMCCEPDVITYNALINCFCKFGKLPIGLEFYREMKGNGLKPNVVSYS 371

Query: 150 VLLYVLVKTGRFELLWRIYEEMISNGLSPSVITFGTLIDGCCRQGDLLRAQEMFDEMRVK 209
            L+    K G  +   + Y +M   GL P+  T+ +LID  C+ G+L  A  + +EM   
Sbjct: 372 TLVDAFCKEGMMQQAIKFYVDMRRVGLVPNEYTYTSLIDANCKIGNLSDAFRLGNEMLQV 431

Query: 210 GIVPTVIVYTILIRGLCSDNKIEEAESMHRAMREVGVYPNVYTYNTLMDGYCKLANAKQA 269
           G+   V+ YT LI GLC   +++EAE +   M   GV PN+ +YN L+ G+ K  N  +A
Sbjct: 432 GVEWNVVTYTALIDGLCDAERMKEAEELFGKMDTAGVIPNLASYNALIHGFVKAKNMDRA 491

Query: 270 LRLYQDMLGEGLVPDVVTFGILIDGLCKFGEMKAARNLFVNMIKFSVTPNIAVYNSLIDA 329
           L L  ++ G G+ PD++ +G  I GLC   +++AA+ +   M +  +  N  +Y +L+DA
Sbjct: 492 LELLNELKGRGIKPDLLLYGTFIWGLCSLEKIEAAKVVMNEMKECGIKANSLIYTTLMDA 551

Query: 330 YCKVGDVSEAMALFLELERFEVSPDVFTYSILIRGLCSVSRTEEAGNIFEKMTKE-GILA 389
           Y K G+ +E + L  E++  ++   V T+ +LI GLC      +A + F +++ + G+ A
Sbjct: 552 YFKSGNPTEGLHLLDEMKELDIEVTVVTFCVLIDGLCKNKLVSKAVDYFNRISNDFGLQA 611

Query: 390 NSVTYNSLIDGCCKEGKMDKALEICSQMTENGVEPNVITFSTLIDGYCKIRNLQAAMGIY 449
           N+  + ++IDG CK+ +++ A  +  QM + G+ P+   +++L+DG  K  N+  A+ + 
Sbjct: 612 NAAIFTAMIDGLCKDNQVEAATTLFEQMVQKGLVPDRTAYTSLMDGNFKQGNVLEALALR 671

Query: 450 SEMVIKSLSPDVVTYTAMIDGHCKYGSMKEALKLYSDMLDNGITPNCYTISCLLDGLCKD 502
            +M    +  D++ YT+++ G      +++A     +M+  GI P+      +L    + 
Sbjct: 672 DKMAEIGMKLDLLAYTSLVWGLSHCNQLQKARSFLEEMIGEGIHPDEVLCISVLKKHYEL 731

BLAST of Cucsa.275720 vs. Swiss-Prot
Match: PPR99_ARATH (Pentatricopeptide repeat-containing protein At1g63130, mitochondrial OS=Arabidopsis thaliana GN=At1g63130 PE=2 SV=1)

HSP 1 Score: 288.5 bits (737), Expect = 1.7e-76
Identity = 160/494 (32.39%), Postives = 261/494 (52.83%), Query Frame = 1

Query: 82  LAFSELSRLKSSKFTPNVYGELIIVLC-----KMELVEEALSMYHKVGAALTIQACNVLL 141
           L  S   ++++   + N+Y   I++ C     ++ L    L+   K+G    I   N LL
Sbjct: 99  LVISLGEQMQNLGISHNLYTYSILINCFCRRSQLSLALAVLAKMMKLGYEPDIVTLNSLL 158

Query: 142 YVLVKTGRFELLWRIYEEMISNGLSPSVITFGTLIDGCCRQGDLLRAQEMFDEMRVKGIV 201
                  R      +  +M+  G  P   TF TLI G  R      A  + D M VKG  
Sbjct: 159 NGFCHGNRISDAVSLVGQMVEMGYQPDSFTFNTLIHGLFRHNRASEAVALVDRMVVKGCQ 218

Query: 202 PTVIVYTILIRGLCSDNKIEEAESMHRAMREVGVYPNVYTYNTLMDGYCKLANAKQALRL 261
           P ++ Y I++ GLC    I+ A S+ + M +  + P V  YNT++D  C   N   AL L
Sbjct: 219 PDLVTYGIVVNGLCKRGDIDLALSLLKKMEQGKIEPGVVIYNTIIDALCNYKNVNDALNL 278

Query: 262 YQDMLGEGLVPDVVTFGILIDGLCKFGEMKAARNLFVNMIKFSVTPNIAVYNSLIDAYCK 321
           + +M  +G+ P+VVT+  LI  LC +G    A  L  +MI+  + PN+  +++LIDA+ K
Sbjct: 279 FTEMDNKGIRPNVVTYNSLIRCLCNYGRWSDASRLLSDMIERKINPNVVTFSALIDAFVK 338

Query: 322 VGDVSEAMALFLELERFEVSPDVFTYSILIRGLCSVSRTEEAGNIFEKMTKEGILANSVT 381
            G + EA  L+ E+ +  + PD+FTYS LI G C   R +EA ++FE M  +    N VT
Sbjct: 339 EGKLVEAEKLYDEMIKRSIDPDIFTYSSLINGFCMHDRLDEAKHMFELMISKDCFPNVVT 398

Query: 382 YNSLIDGCCKEGKMDKALEICSQMTENGVEPNVITFSTLIDGYCKIRNLQAAMGIYSEMV 441
           YN+LI G CK  ++D+ +E+  +M++ G+  N +T++TLI G+ + R    A  ++ +MV
Sbjct: 399 YNTLIKGFCKAKRVDEGMELFREMSQRGLVGNTVTYTTLIHGFFQARECDNAQIVFKQMV 458

Query: 442 IKSLSPDVVTYTAMIDGHCKYGSMKEALKLYSDMLDNGITPNCYTISCLLDGLCKDGKIS 501
              + PD++TY+ ++DG C  G ++ AL ++  +  + + P+ YT + +++G+CK GK+ 
Sbjct: 459 SDGVLPDIMTYSILLDGLCNNGKVETALVVFEYLQRSKMEPDIYTYNIMIEGMCKAGKVE 518

Query: 502 DALELFTEKIEFQTPRCNVDAGGSKPSLTNHVAYTALIHGLCQDGQFSKAVKLFSDMRRY 561
           D  +LF          C++   G KP   N V YT ++ G C+ G   +A  LF +M+  
Sbjct: 519 DGWDLF----------CSLSLKGVKP---NVVTYTTMMSGFCRKGLKEEADALFREMKEE 578

Query: 562 GLQPDEVIYVVMLR 571
           G  PD   Y  ++R
Sbjct: 579 GPLPDSGTYNTLIR 579

HSP 2 Score: 215.3 bits (547), Expect = 1.9e-54
Identity = 123/434 (28.34%), Postives = 222/434 (51.15%), Query Frame = 1

Query: 183 AQEMFDEMRVKGIVPTVIVYTILIRGLCSDNKIEEAESMHRAMREVGVYPNVYTYNTLMD 242
           A  +F +M      P+++ ++ L+  +   NK +   S+   M+ +G+  N+YTY+ L++
Sbjct: 65  AVNLFGDMVKSRPFPSIVEFSKLLSAIAKMNKFDLVISLGEQMQNLGISHNLYTYSILIN 124

Query: 243 GYCKLANAKQALRLYQDMLGEGLVPDVVTFGILIDGLCKFGEMKAARNLFVNMIKFSVTP 302
            +C+ +    AL +   M+  G  PD+VT   L++G C    +  A +L   M++    P
Sbjct: 125 CFCRRSQLSLALAVLAKMMKLGYEPDIVTLNSLLNGFCHGNRISDAVSLVGQMVEMGYQP 184

Query: 303 NIAVYNSLIDAYCKVGDVSEAMALFLELERFEVSPDVFTYSILIRGLCSVSRTEEAGNIF 362
           +   +N+LI    +    SEA+AL   +      PD+ TY I++ GLC     + A ++ 
Sbjct: 185 DSFTFNTLIHGLFRHNRASEAVALVDRMVVKGCQPDLVTYGIVVNGLCKRGDIDLALSLL 244

Query: 363 EKMTKEGILANSVTYNSLIDGCCKEGKMDKALEICSQMTENGVEPNVITFSTLIDGYCKI 422
           +KM +  I    V YN++ID  C    ++ AL + ++M   G+ PNV+T+++LI   C  
Sbjct: 245 KKMEQGKIEPGVVIYNTIIDALCNYKNVNDALNLFTEMDNKGIRPNVVTYNSLIRCLCNY 304

Query: 423 RNLQAAMGIYSEMVIKSLSPDVVTYTAMIDGHCKYGSMKEALKLYSDMLDNGITPNCYTI 482
                A  + S+M+ + ++P+VVT++A+ID   K G + EA KLY +M+   I P+ +T 
Sbjct: 305 GRWSDASRLLSDMIERKINPNVVTFSALIDAFVKEGKLVEAEKLYDEMIKRSIDPDIFTY 364

Query: 483 SCLLDGLCKDGKISDALELFTEKIEFQTPRCNVDAGGSKPSLTNHVAYTALIHGLCQDGQ 542
           S L++G C   ++ +A  +F   I             SK    N V Y  LI G C+  +
Sbjct: 365 SSLINGFCMHDRLDEAKHMFELMI-------------SKDCFPNVVTYNTLIKGFCKAKR 424

Query: 543 FSKAVKLFSDMRRYGLQPDEVIYVVMLRGLFQVKYI--LMMLHADMLKFGVIPNSAVHVI 602
             + ++LF +M + GL  + V Y  ++ G FQ +      ++   M+  GV+P+   + I
Sbjct: 425 VDEGMELFREMSQRGLVGNTVTYTTLIHGFFQARECDNAQIVFKQMVSDGVLPDIMTYSI 484

Query: 603 LCECYQESGFLKSA 615
           L +    +G +++A
Sbjct: 485 LLDGLCNNGKVETA 485

HSP 3 Score: 191.8 bits (486), Expect = 2.2e-47
Identity = 112/416 (26.92%), Postives = 204/416 (49.04%), Query Frame = 1

Query: 82  LAFSELSRLKSSKFTPNV--YGELIIVLCKMELVEEALSMYHKVGAALTIQACNVLLYVL 141
           LA S L +++  K  P V  Y  +I  LC  + V +AL+++                   
Sbjct: 239 LALSLLKKMEQGKIEPGVVIYNTIIDALCNYKNVNDALNLF------------------- 298

Query: 142 VKTGRFELLWRIYEEMISNGLSPSVITFGTLIDGCCRQGDLLRAQEMFDEMRVKGIVPTV 201
                         EM + G+ P+V+T+ +LI   C  G    A  +  +M  + I P V
Sbjct: 299 -------------TEMDNKGIRPNVVTYNSLIRCLCNYGRWSDASRLLSDMIERKINPNV 358

Query: 202 IVYTILIRGLCSDNKIEEAESMHRAMREVGVYPNVYTYNTLMDGYCKLANAKQALRLYQD 261
           + ++ LI     + K+ EAE ++  M +  + P+++TY++L++G+C      +A  +++ 
Sbjct: 359 VTFSALIDAFVKEGKLVEAEKLYDEMIKRSIDPDIFTYSSLINGFCMHDRLDEAKHMFEL 418

Query: 262 MLGEGLVPDVVTFGILIDGLCKFGEMKAARNLFVNMIKFSVTPNIAVYNSLIDAYCKVGD 321
           M+ +   P+VVT+  LI G CK   +     LF  M +  +  N   Y +LI  + +  +
Sbjct: 419 MISKDCFPNVVTYNTLIKGFCKAKRVDEGMELFREMSQRGLVGNTVTYTTLIHGFFQARE 478

Query: 322 VSEAMALFLELERFEVSPDVFTYSILIRGLCSVSRTEEAGNIFEKMTKEGILANSVTYNS 381
              A  +F ++    V PD+ TYSIL+ GLC+  + E A  +FE + +  +  +  TYN 
Sbjct: 479 CDNAQIVFKQMVSDGVLPDIMTYSILLDGLCNNGKVETALVVFEYLQRSKMEPDIYTYNI 538

Query: 382 LIDGCCKEGKMDKALEICSQMTENGVEPNVITFSTLIDGYCKIRNLQAAMGIYSEMVIKS 441
           +I+G CK GK++   ++   ++  GV+PNV+T++T++ G+C+    + A  ++ EM  + 
Sbjct: 539 MIEGMCKAGKVEDGWDLFCSLSLKGVKPNVVTYTTMMSGFCRKGLKEEADALFREMKEEG 598

Query: 442 LSPDVVTYTAMIDGHCKYGSMKEALKLYSDMLDNGITPNCYTISCLLDGLCKDGKI 496
             PD  TY  +I  H + G    + +L  +M       +  TI  L+  +  DG++
Sbjct: 599 PLPDSGTYNTLIRAHLRDGDKAASAELIREMRSCRFVGDASTIG-LVTNMLHDGRL 621

HSP 4 Score: 116.7 bits (291), Expect = 9.0e-25
Identity = 91/356 (25.56%), Postives = 163/356 (45.79%), Query Frame = 1

Query: 13  DSLITTVLNCRSPWKALEFF----NAAPEKNIQLYSAIIHVLVG-------SKLLSHAR- 72
           +++I  + N ++   AL  F    N     N+  Y+++I  L         S+LLS    
Sbjct: 260 NTIIDALCNYKNVNDALNLFTEMDNKGIRPNVVTYNSLIRCLCNYGRWSDASRLLSDMIE 319

Query: 73  -------YLLNDLVQNLVKSHKPYHACQLAFSELSR-LKSSKFTPNVYGELIIVLCKMEL 132
                     + L+   VK  K   A +L    + R +    FT   Y  LI   C  + 
Sbjct: 320 RKINPNVVTFSALIDAFVKEGKLVEAEKLYDEMIKRSIDPDIFT---YSSLINGFCMHDR 379

Query: 133 VEEALSMYHKV---GAALTIQACNVLLYVLVKTGRFELLWRIYEEMISNGLSPSVITFGT 192
           ++EA  M+  +        +   N L+    K  R +    ++ EM   GL  + +T+ T
Sbjct: 380 LDEAKHMFELMISKDCFPNVVTYNTLIKGFCKAKRVDEGMELFREMSQRGLVGNTVTYTT 439

Query: 193 LIDGCCRQGDLLRAQEMFDEMRVKGIVPTVIVYTILIRGLCSDNKIEEAESMHRAMREVG 252
           LI G  +  +   AQ +F +M   G++P ++ Y+IL+ GLC++ K+E A  +   ++   
Sbjct: 440 LIHGFFQARECDNAQIVFKQMVSDGVLPDIMTYSILLDGLCNNGKVETALVVFEYLQRSK 499

Query: 253 VYPNVYTYNTLMDGYCKLANAKQALRLYQDMLGEGLVPDVVTFGILIDGLCKFGEMKAAR 312
           + P++YTYN +++G CK    +    L+  +  +G+ P+VVT+  ++ G C+ G  + A 
Sbjct: 500 MEPDIYTYNIMIEGMCKAGKVEDGWDLFCSLSLKGVKPNVVTYTTMMSGFCRKGLKEEAD 559

Query: 313 NLFVNMIKFSVTPNIAVYNSLIDAYCKVGDVSEAMALFLELERFEVSPDVFTYSIL 346
            LF  M +    P+   YN+LI A+ + GD + +  L  E+       D  T  ++
Sbjct: 560 ALFREMKEEGPLPDSGTYNTLIRAHLRDGDKAASAELIREMRSCRFVGDASTIGLV 612

BLAST of Cucsa.275720 vs. Swiss-Prot
Match: PPR38_ARATH (Putative pentatricopeptide repeat-containing protein At1g12700, mitochondrial OS=Arabidopsis thaliana GN=At1g12700 PE=3 SV=1)

HSP 1 Score: 277.7 bits (709), Expect = 3.1e-73
Identity = 150/500 (30.00%), Postives = 260/500 (52.00%), Query Frame = 1

Query: 86  ELSRLKSSKFTPNVYGELIIVLCKMELVEEALSMYHKVGAALTIQACNVLLYVLVKTGRF 145
           EL+ +  + +T N+        CK       L    K+G        N L+  L   G+ 
Sbjct: 115 ELNGIAHNIYTLNIMINCFCRCCKTCFAYSVLGKVMKLGYEPDTTTFNTLIKGLFLEGKV 174

Query: 146 ELLWRIYEEMISNGLSPSVITFGTLIDGCCRQGDLLRAQEMFDEMRVKGIVPTVIVYTIL 205
                + + M+ NG  P V+T+ ++++G CR GD   A ++  +M  + +   V  Y+ +
Sbjct: 175 SEAVVLVDRMVENGCQPDVVTYNSIVNGICRSGDTSLALDLLRKMEERNVKADVFTYSTI 234

Query: 206 IRGLCSDNKIEEAESMHRAMREVGVYPNVYTYNTLMDGYCKLANAKQALRLYQDMLGEGL 265
           I  LC D  I+ A S+ + M   G+  +V TYN+L+ G CK         L +DM+   +
Sbjct: 235 IDSLCRDGCIDAAISLFKEMETKGIKSSVVTYNSLVRGLCKAGKWNDGALLLKDMVSREI 294

Query: 266 VPDVVTFGILIDGLCKFGEMKAARNLFVNMIKFSVTPNIAVYNSLIDAYCKVGDVSEAMA 325
           VP+V+TF +L+D   K G+++ A  L+  MI   ++PNI  YN+L+D YC    +SEA  
Sbjct: 295 VPNVITFNVLLDVFVKEGKLQEANELYKEMITRGISPNIITYNTLMDGYCMQNRLSEANN 354

Query: 326 LFLELERFEVSPDVFTYSILIRGLCSVSRTEEAGNIFEKMTKEGILANSVTYNSLIDGCC 385
           +   + R + SPD+ T++ LI+G C V R ++   +F  ++K G++AN+VTY+ L+ G C
Sbjct: 355 MLDLMVRNKCSPDIVTFTSLIKGYCMVKRVDDGMKVFRNISKRGLVANAVTYSILVQGFC 414

Query: 386 KEGKMDKALEICSQMTENGVEPNVITFSTLIDGYCKIRNLQAAMGIYSEMVIKSLSPDVV 445
           + GK+  A E+  +M  +GV P+V+T+  L+DG C    L+ A+ I+ ++    +   +V
Sbjct: 415 QSGKIKLAEELFQEMVSHGVLPDVMTYGILLDGLCDNGKLEKALEIFEDLQKSKMDLGIV 474

Query: 446 TYTAMIDGHCKYGSMKEALKLYSDMLDNGITPNCYTISCLLDGLCKDGKISDALELFTEK 505
            YT +I+G CK G +++A  L+  +   G+ PN  T + ++ GLCK G +S+A  L  + 
Sbjct: 475 MYTTIIEGMCKGGKVEDAWNLFCSLPCKGVKPNVMTYTVMISGLCKKGSLSEANILLRK- 534

Query: 506 IEFQTPRCNVDAGGSKPSLTNHVAYTALIHGLCQDGQFSKAVKLFSDMRRYGLQPD---- 565
                    ++  G+ P   N   Y  LI    +DG  + + KL  +M+  G   D    
Sbjct: 535 ---------MEEDGNAP---NDCTYNTLIRAHLRDGDLTASAKLIEEMKSCGFSADASSI 594

Query: 566 EVIYVVMLRGLFQVKYILMM 582
           +++  ++L G     ++ M+
Sbjct: 595 KMVIDMLLSGELDKSFLDML 601

HSP 2 Score: 176.0 bits (445), Expect = 1.3e-42
Identity = 102/405 (25.19%), Postives = 203/405 (50.12%), Query Frame = 1

Query: 212 DNKIEEAESMHRAMREVGVYPNVYTYNTLMDGYCKLANAKQALRLYQDMLGEGLVPDVVT 271
           D K ++A ++ + M      P++  ++       +       L   + +   G+  ++ T
Sbjct: 66  DIKKDDAIALFQEMIRSRPLPSLVDFSRFFSAIARTKQFNLVLDFCKQLELNGIAHNIYT 125

Query: 272 FGILIDGLCKFGEMKAARNLFVNMIKFSVTPNIAVYNSLIDAYCKVGDVSEAMALFLELE 331
             I+I+  C+  +   A ++   ++K    P+   +N+LI      G VSEA+ L   + 
Sbjct: 126 LNIMINCFCRCCKTCFAYSVLGKVMKLGYEPDTTTFNTLIKGLFLEGKVSEAVVLVDRMV 185

Query: 332 RFEVSPDVFTYSILIRGLCSVSRTEEAGNIFEKMTKEGILANSVTYNSLIDGCCKEGKMD 391
                PDV TY+ ++ G+C    T  A ++  KM +  + A+  TY+++ID  C++G +D
Sbjct: 186 ENGCQPDVVTYNSIVNGICRSGDTSLALDLLRKMEERNVKADVFTYSTIIDSLCRDGCID 245

Query: 392 KALEICSQMTENGVEPNVITFSTLIDGYCKIRNLQAAMGIYSEMVIKSLSPDVVTYTAMI 451
            A+ +  +M   G++ +V+T+++L+ G CK         +  +MV + + P+V+T+  ++
Sbjct: 246 AAISLFKEMETKGIKSSVVTYNSLVRGLCKAGKWNDGALLLKDMVSREIVPNVITFNVLL 305

Query: 452 DGHCKYGSMKEALKLYSDMLDNGITPNCYTISCLLDGLCKDGKISDALELFTEKIEFQTP 511
           D   K G ++EA +LY +M+  GI+PN  T + L+DG C   ++S+A  +    +     
Sbjct: 306 DVFVKEGKLQEANELYKEMITRGISPNIITYNTLMDGYCMQNRLSEANNMLDLMVR---N 365

Query: 512 RCNVDAGGSKPSLTNHVAYTALIHGLCQDGQFSKAVKLFSDMRRYGLQPDEVIYVVMLRG 571
           +C+ D           V +T+LI G C   +    +K+F ++ + GL  + V Y ++++G
Sbjct: 366 KCSPDI----------VTFTSLIKGYCMVKRVDDGMKVFRNISKRGLVANAVTYSILVQG 425

Query: 572 LFQVKYILMM--LHADMLKFGVIPNSAVHVILCECYQESGFLKSA 615
             Q   I +   L  +M+  GV+P+   + IL +   ++G L+ A
Sbjct: 426 FCQSGKIKLAEELFQEMVSHGVLPDVMTYGILLDGLCDNGKLEKA 457

HSP 3 Score: 106.7 bits (265), Expect = 9.3e-22
Identity = 77/329 (23.40%), Postives = 149/329 (45.29%), Query Frame = 1

Query: 295 MIKFSVTPNIAVYNSLIDAYCKVGDVSEAM-----ALFLELER--FEVSPDVFTYSILIR 354
           MIK S+T N+     +     K G +   +     + F   ER    +S     +   +R
Sbjct: 2   MIKRSITTNMKALRLIQPHLLKTGSLRTDLLCTISSFFSSCERDFSSISNGNVCFRERLR 61

Query: 355 GLCSVSRTEEAGNIFEKMTKEGILANSVTYNSLIDGCCKEGKMDKALEICSQMTENGVEP 414
                 + ++A  +F++M +   L + V ++       +  + +  L+ C Q+  NG+  
Sbjct: 62  SGIVDIKKDDAIALFQEMIRSRPLPSLVDFSRFFSAIARTKQFNLVLDFCKQLELNGIAH 121

Query: 415 NVITFSTLIDGYCKIRNLQAAMGIYSEMVIKSLSPDVVTYTAMIDGHCKYGSMKEALKLY 474
           N+ T + +I+ +C+      A  +  +++     PD  T+  +I G    G + EA+ L 
Sbjct: 122 NIYTLNIMINCFCRCCKTCFAYSVLGKVMKLGYEPDTTTFNTLIKGLFLEGKVSEAVVLV 181

Query: 475 SDMLDNGITPNCYTISCLLDGLCKDGKISDALELFTEKIEFQTPRCNVDAGGSKPSLTNH 534
             M++NG  P+  T + +++G+C+ G  S AL+L   K+E +  + +V            
Sbjct: 182 DRMVENGCQPDVVTYNSIVNGICRSGDTSLALDLL-RKMEERNVKADV------------ 241

Query: 535 VAYTALIHGLCQDGQFSKAVKLFSDMRRYGLQPDEVIYVVMLRGLFQVKYIL--MMLHAD 594
             Y+ +I  LC+DG    A+ LF +M   G++   V Y  ++RGL +        +L  D
Sbjct: 242 FTYSTIIDSLCRDGCIDAAISLFKEMETKGIKSSVVTYNSLVRGLCKAGKWNDGALLLKD 301

Query: 595 MLKFGVIPNSAVHVILCECYQESGFLKSA 615
           M+   ++PN     +L + + + G L+ A
Sbjct: 302 MVSREIVPNVITFNVLLDVFVKEGKLQEA 317

BLAST of Cucsa.275720 vs. Swiss-Prot
Match: PP437_ARATH (Putative pentatricopeptide repeat-containing protein At5g59900 OS=Arabidopsis thaliana GN=At5g59900 PE=3 SV=1)

HSP 1 Score: 277.3 bits (708), Expect = 4.0e-73
Identity = 153/423 (36.17%), Postives = 234/423 (55.32%), Query Frame = 1

Query: 151 IYEEMISN-GLSPSVITFGTLIDGCCRQGDLLRAQEMFDEMRVKGIVPTVIVYTILIRGL 210
           +++ MI+   L P V T   L+ G  +      A E+F++M   GI P V +YT +IR L
Sbjct: 178 VFKMMITKVSLLPEVRTLSALLHGLVKFRHFGLAMELFNDMVSVGIRPDVYIYTGVIRSL 237

Query: 211 CSDNKIEEAESMHRAMREVGVYPNVYTYNTLMDGYCKLANAKQALRLYQDMLGEGLVPDV 270
           C    +  A+ M   M   G   N+  YN L+DG CK     +A+ + +D+ G+ L PDV
Sbjct: 238 CELKDLSRAKEMIAHMEATGCDVNIVPYNVLIDGLCKKQKVWEAVGIKKDLAGKDLKPDV 297

Query: 271 VTFGILIDGLCKFGEMKAARNLFVNMIKFSVTPNIAVYNSLIDAYCKVGDVSEAMALFLE 330
           VT+  L+ GLCK  E +    +   M+    +P+ A  +SL++   K G + EA+ L   
Sbjct: 298 VTYCTLVYGLCKVQEFEIGLEMMDEMLCLRFSPSEAAVSSLVEGLRKRGKIEEALNLVKR 357

Query: 331 LERFEVSPDVFTYSILIRGLCSVSRTEEAGNIFEKMTKEGILANSVTYNSLIDGCCKEGK 390
           +  F VSP++F Y+ LI  LC   +  EA  +F++M K G+  N VTY+ LID  C+ GK
Sbjct: 358 VVDFGVSPNLFVYNALIDSLCKGRKFHEAELLFDRMGKIGLRPNDVTYSILIDMFCRRGK 417

Query: 391 MDKALEICSQMTENGVEPNVITFSTLIDGYCKIRNLQAAMGIYSEMVIKSLSPDVVTYTA 450
           +D AL    +M + G++ +V  +++LI+G+CK  ++ AA G  +EM+ K L P VVTYT+
Sbjct: 418 LDTALSFLGEMVDTGLKLSVYPYNSLINGHCKFGDISAAEGFMAEMINKKLEPTVVTYTS 477

Query: 451 MIDGHCKYGSMKEALKLYSDMLDNGITPNCYTISCLLDGLCKDGKISDALELFTEKIEFQ 510
           ++ G+C  G + +AL+LY +M   GI P+ YT + LL GL + G I DA++LF E  E+ 
Sbjct: 478 LMGGYCSKGKINKALRLYHEMTGKGIAPSIYTFTTLLSGLFRAGLIRDAVKLFNEMAEWN 537

Query: 511 TPRCNVDAGGSKPSLTNHVAYTALIHGLCQDGQFSKAVKLFSDMRRYGLQPDEVIYVVML 570
                      KP   N V Y  +I G C++G  SKA +   +M   G+ PD   Y  ++
Sbjct: 538 V----------KP---NRVTYNVMIEGYCEEGDMSKAFEFLKEMTEKGIVPDTYSYRPLI 587

Query: 571 RGL 573
            GL
Sbjct: 598 HGL 587

HSP 2 Score: 258.5 bits (659), Expect = 1.9e-67
Identity = 166/554 (29.96%), Postives = 273/554 (49.28%), Query Frame = 1

Query: 25  PWKALEFFNAAP-----EKNIQLYSAIIHVLVGSKLLSHARYLLNDLVQNLVKSHKPYHA 84
           P   L FFN        + +   +  +IH LV + L   A  LL  L   L+++ KP   
Sbjct: 84  PKLGLRFFNFLGLHRGFDHSTASFCILIHALVKANLFWPASSLLQTL---LLRALKPSDV 143

Query: 85  CQLAFS--ELSRLKSSKFTPNVYGELIIVLCKMELVEEALSMYHKVGAALTIQACNVLLY 144
             + FS  E  +L SS     +    +     ++ V     M  KV     ++  + LL+
Sbjct: 144 FNVLFSCYEKCKLSSSSSFDLLIQHYVRSRRVLDGVLVFKMMITKVSLLPEVRTLSALLH 203

Query: 145 VLVKTGRFELLWRIYEEMISNGLSPSVITFGTLIDGCCRQGDLLRAQEMFDEMRVKGIVP 204
            LVK   F L   ++ +M+S G+ P V  +  +I   C   DL RA+EM   M   G   
Sbjct: 204 GLVKFRHFGLAMELFNDMVSVGIRPDVYIYTGVIRSLCELKDLSRAKEMIAHMEATGCDV 263

Query: 205 TVIVYTILIRGLCSDNKIEEAESMHRAMREVGVYPNVYTYNTLMDGYCKLANAKQALRLY 264
            ++ Y +LI GLC   K+ EA  + + +    + P+V TY TL+ G CK+   +  L + 
Sbjct: 264 NIVPYNVLIDGLCKKQKVWEAVGIKKDLAGKDLKPDVVTYCTLVYGLCKVQEFEIGLEMM 323

Query: 265 QDMLGEGLVPDVVTFGILIDGLCKFGEMKAARNLFVNMIKFSVTPNIAVYNSLIDAYCKV 324
            +ML     P       L++GL K G+++ A NL   ++ F V+PN+ VYN+LID+ CK 
Sbjct: 324 DEMLCLRFSPSEAAVSSLVEGLRKRGKIEEALNLVKRVVDFGVSPNLFVYNALIDSLCKG 383

Query: 325 GDVSEAMALFLELERFEVSPDVFTYSILIRGLCSVSRTEEAGNIFEKMTKEGILANSVTY 384
               EA  LF  + +  + P+  TYSILI   C   + + A +   +M   G+  +   Y
Sbjct: 384 RKFHEAELLFDRMGKIGLRPNDVTYSILIDMFCRRGKLDTALSFLGEMVDTGLKLSVYPY 443

Query: 385 NSLIDGCCKEGKMDKALEICSQMTENGVEPNVITFSTLIDGYCKIRNLQAAMGIYSEMVI 444
           NSLI+G CK G +  A    ++M    +EP V+T+++L+ GYC    +  A+ +Y EM  
Sbjct: 444 NSLINGHCKFGDISAAEGFMAEMINKKLEPTVVTYTSLMGGYCSKGKINKALRLYHEMTG 503

Query: 445 KSLSPDVVTYTAMIDGHCKYGSMKEALKLYSDMLDNGITPNCYTISCLLDGLCKDGKISD 504
           K ++P + T+T ++ G  + G +++A+KL+++M +  + PN  T + +++G C++G +S 
Sbjct: 504 KGIAPSIYTFTTLLSGLFRAGLIRDAVKLFNEMAEWNVKPNRVTYNVMIEGYCEEGDMSK 563

Query: 505 ALELFTEKIEFQTPRCNVDAGGSKPSLTNHVAYTALIHGLCQDGQFSKAVKLFSDMRRYG 564
           A E   E  E             K  + +  +Y  LIHGLC  GQ S+A      + +  
Sbjct: 564 AFEFLKEMTE-------------KGIVPDTYSYRPLIHGLCLTGQASEAKVFVDGLHKGN 621

Query: 565 LQPDEVIYVVMLRG 572
            + +E+ Y  +L G
Sbjct: 624 CELNEICYTGLLHG 621

HSP 3 Score: 257.7 bits (657), Expect = 3.3e-67
Identity = 157/497 (31.59%), Postives = 254/497 (51.11%), Query Frame = 1

Query: 81  QLAFSELSRLKSSKFTPN--VYGELIIVLCKMELVEEALSMYHKV---GAALTIQACNVL 140
           ++    +  +   +F+P+      L+  L K   +EEAL++  +V   G +  +   N L
Sbjct: 314 EIGLEMMDEMLCLRFSPSEAAVSSLVEGLRKRGKIEEALNLVKRVVDFGVSPNLFVYNAL 373

Query: 141 LYVLVKTGRFELLWRIYEEMISNGLSPSVITFGTLIDGCCRQGDLLRAQEMFDEMRVKGI 200
           +  L K  +F     +++ M   GL P+ +T+  LID  CR+G L  A     EM   G+
Sbjct: 374 IDSLCKGRKFHEAELLFDRMGKIGLRPNDVTYSILIDMFCRRGKLDTALSFLGEMVDTGL 433

Query: 201 VPTVIVYTILIRGLCSDNKIEEAESMHRAMREVGVYPNVYTYNTLMDGYCKLANAKQALR 260
             +V  Y  LI G C    I  AE     M    + P V TY +LM GYC      +ALR
Sbjct: 434 KLSVYPYNSLINGHCKFGDISAAEGFMAEMINKKLEPTVVTYTSLMGGYCSKGKINKALR 493

Query: 261 LYQDMLGEGLVPDVVTFGILIDGLCKFGEMKAARNLFVNMIKFSVTPNIAVYNSLIDAYC 320
           LY +M G+G+ P + TF  L+ GL + G ++ A  LF  M +++V PN   YN +I+ YC
Sbjct: 494 LYHEMTGKGIAPSIYTFTTLLSGLFRAGLIRDAVKLFNEMAEWNVKPNRVTYNVMIEGYC 553

Query: 321 KVGDVSEAMALFLELERFEVSPDVFTYSILIRGLCSVSRTEEAGNIFEKMTKEGILANSV 380
           + GD+S+A     E+    + PD ++Y  LI GLC   +  EA    + + K     N +
Sbjct: 554 EEGDMSKAFEFLKEMTEKGIVPDTYSYRPLIHGLCLTGQASEAKVFVDGLHKGNCELNEI 613

Query: 381 TYNSLIDGCCKEGKMDKALEICSQMTENGVEPNVITFSTLIDGYCKIRNLQAAMGIYSEM 440
            Y  L+ G C+EGK+++AL +C +M + GV+ +++ +  LIDG  K ++ +   G+  EM
Sbjct: 614 CYTGLLHGFCREGKLEEALSVCQEMVQRGVDLDLVCYGVLIDGSLKHKDRKLFFGLLKEM 673

Query: 441 VIKSLSPDVVTYTAMIDGHCKYGSMKEALKLYSDMLDNGITPNCYTISCLLDGLCKDGKI 500
             + L PD V YT+MID   K G  KEA  ++  M++ G  PN  T + +++GLCK G +
Sbjct: 674 HDRGLKPDDVIYTSMIDAKSKTGDFKEAFGIWDLMINEGCVPNEVTYTAVINGLCKAGFV 733

Query: 501 SDALELFTEKIEFQTPRCNVDAGGSKPSLTNHVAYTALIHGLCQ-DGQFSKAVKLFSDMR 560
           ++A E+   K++               S+ N V Y   +  L + +    KAV+L + + 
Sbjct: 734 NEA-EVLCSKMQ------------PVSSVPNQVTYGCFLDILTKGEVDMQKAVELHNAIL 793

Query: 561 RYGLQPDEVIYVVMLRG 572
           + GL  +   Y +++RG
Sbjct: 794 K-GLLANTATYNMLIRG 796

HSP 4 Score: 241.5 bits (615), Expect = 2.4e-62
Identity = 180/635 (28.35%), Postives = 304/635 (47.87%), Query Frame = 1

Query: 1   MLQVVSLSSLTPDSLITTVLNCRSPWK--ALEFFNAAPEKNIQLYSAIIHVLVGSKLLSH 60
           +LQ + L +L P  +   + +C    K  +   F+   +  ++    +  VLV   +++ 
Sbjct: 126 LLQTLLLRALKPSDVFNVLFSCYEKCKLSSSSSFDLLIQHYVRSRRVLDGVLVFKMMITK 185

Query: 61  ARYL-----LNDLVQNLVKSHKPYHACQLAFSELSRLKSSKFTPNVY--GELIIVLCKME 120
              L     L+ L+  LVK    +    LA    + + S    P+VY    +I  LC+++
Sbjct: 186 VSLLPEVRTLSALLHGLVK----FRHFGLAMELFNDMVSVGIRPDVYIYTGVIRSLCELK 245

Query: 121 LVEEALSMY---HKVGAALTIQACNVLLYVLVKTGRFELLWRIYEEMISNGLSPSVITFG 180
            +  A  M       G  + I   NVL+  L K  +      I +++    L P V+T+ 
Sbjct: 246 DLSRAKEMIAHMEATGCDVNIVPYNVLIDGLCKKQKVWEAVGIKKDLAGKDLKPDVVTYC 305

Query: 181 TLIDGCCRQGDLLRAQEMFDEMRVKGIVPTVIVYTILIRGLCSDNKIEEAESMHRAMREV 240
           TL+ G C+  +     EM DEM      P+    + L+ GL    KIEEA ++ + + + 
Sbjct: 306 TLVYGLCKVQEFEIGLEMMDEMLCLRFSPSEAAVSSLVEGLRKRGKIEEALNLVKRVVDF 365

Query: 241 GVYPNVYTYNTLMDGYCKLANAKQALRLYQDMLGEGLVPDVVTFGILIDGLCKFGEMKAA 300
           GV PN++ YN L+D  CK     +A  L+  M   GL P+ VT+ ILID  C+ G++  A
Sbjct: 366 GVSPNLFVYNALIDSLCKGRKFHEAELLFDRMGKIGLRPNDVTYSILIDMFCRRGKLDTA 425

Query: 301 RNLFVNMIKFSVTPNIAVYNSLIDAYCKVGDVSEAMALFLELERFEVSPDVFTYSILIRG 360
            +    M+   +  ++  YNSLI+ +CK GD+S A     E+   ++ P V TY+ L+ G
Sbjct: 426 LSFLGEMVDTGLKLSVYPYNSLINGHCKFGDISAAEGFMAEMINKKLEPTVVTYTSLMGG 485

Query: 361 LCSVSRTEEAGNIFEKMTKEGILANSVTYNSLIDGCCKEGKMDKALEICSQMTENGVEPN 420
            CS  +  +A  ++ +MT +GI  +  T+ +L+ G  + G +  A+++ ++M E  V+PN
Sbjct: 486 YCSKGKINKALRLYHEMTGKGIAPSIYTFTTLLSGLFRAGLIRDAVKLFNEMAEWNVKPN 545

Query: 421 VITFSTLIDGYCKIRNLQAAMGIYSEMVIKSLSPDVVTYTAMIDGHCKYGSMKEALKLYS 480
            +T++ +I+GYC+  ++  A     EM  K + PD  +Y  +I G C  G   EA K++ 
Sbjct: 546 RVTYNVMIEGYCEEGDMSKAFEFLKEMTEKGIVPDTYSYRPLIHGLCLTGQASEA-KVFV 605

Query: 481 DMLDNG---ITPNCYTISCLLDGLCKDGKISDALELFTEKIEFQTPRCNVDAGGSKPSLT 540
           D L  G   +   CYT   LL G C++GK+ +AL +  E ++       VD         
Sbjct: 606 DGLHKGNCELNEICYT--GLLHGFCREGKLEEALSVCQEMVQ-----RGVD--------L 665

Query: 541 NHVAYTALIHGLCQDGQFSKAVKLFSDMRRYGLQPDEVIYVVML-----RGLFQVKYILM 600
           + V Y  LI G  +         L  +M   GL+PD+VIY  M+      G F+  + + 
Sbjct: 666 DLVCYGVLIDGSLKHKDRKLFFGLLKEMHDRGLKPDDVIYTSMIDAKSKTGDFKEAFGIW 725

Query: 601 MLHADMLKFGVIPNSAVHVILCECYQESGFLKSAQ 616
            L   M+  G +PN   +  +     ++GF+  A+
Sbjct: 726 DL---MINEGCVPNEVTYTAVINGLCKAGFVNEAE 737

HSP 5 Score: 235.7 bits (600), Expect = 1.3e-60
Identity = 157/544 (28.86%), Postives = 269/544 (49.45%), Query Frame = 1

Query: 61  YLLNDLVQNLVKSHKPYHACQLAFSELSRLKSSKFTPN--VYGELIIVLCKMELVEEALS 120
           ++ N L+ +L K  K +H  +L F  + ++      PN   Y  LI + C+   ++ ALS
Sbjct: 368 FVYNALIDSLCKGRK-FHEAELLFDRMGKIG---LRPNDVTYSILIDMFCRRGKLDTALS 427

Query: 121 MYHKV---GAALTIQACNVLLYVLVKTGRFELLWRIYEEMISNGLSPSVITFGTLIDGCC 180
              ++   G  L++   N L+    K G          EMI+  L P+V+T+ +L+ G C
Sbjct: 428 FLGEMVDTGLKLSVYPYNSLINGHCKFGDISAAEGFMAEMINKKLEPTVVTYTSLMGGYC 487

Query: 181 RQGDLLRAQEMFDEMRVKGIVPTVIVYTILIRGLCSDNKIEEAESMHRAMREVGVYPNVY 240
            +G + +A  ++ EM  KGI P++  +T L+ GL     I +A  +   M E  V PN  
Sbjct: 488 SKGKINKALRLYHEMTGKGIAPSIYTFTTLLSGLFRAGLIRDAVKLFNEMAEWNVKPNRV 547

Query: 241 TYNTLMDGYCKLANAKQALRLYQDMLGEGLVPDVVTFGILIDGLCKFGEMKAARNLFVNM 300
           TYN +++GYC+  +  +A    ++M  +G+VPD  ++  LI GLC  G+   A+     +
Sbjct: 548 TYNVMIEGYCEEGDMSKAFEFLKEMTEKGIVPDTYSYRPLIHGLCLTGQASEAKVFVDGL 607

Query: 301 IKFSVTPNIAVYNSLIDAYCKVGDVSEAMALFLELERFEVSPDVFTYSILIRGLCSVSRT 360
            K +   N   Y  L+  +C+ G + EA+++  E+ +  V  D+  Y +LI G       
Sbjct: 608 HKGNCELNEICYTGLLHGFCREGKLEEALSVCQEMVQRGVDLDLVCYGVLIDGSLKHKDR 667

Query: 361 EEAGNIFEKMTKEGILANSVTYNSLIDGCCKEGKMDKALEICSQMTENGVEPNVITFSTL 420
           +    + ++M   G+  + V Y S+ID   K G   +A  I   M   G  PN +T++ +
Sbjct: 668 KLFFGLLKEMHDRGLKPDDVIYTSMIDAKSKTGDFKEAFGIWDLMINEGCVPNEVTYTAV 727

Query: 421 IDGYCKIRNLQAAMGIYSEMVIKSLSPDVVTYTAMIDGHCKYG-SMKEALKLYSDMLDNG 480
           I+G CK   +  A  + S+M   S  P+ VTY   +D   K    M++A++L++ +L  G
Sbjct: 728 INGLCKAGFVNEAEVLCSKMQPVSSVPNQVTYGCFLDILTKGEVDMQKAVELHNAIL-KG 787

Query: 481 ITPNCYTISCLLDGLCKDGKISDALELFTEKI-EFQTPRCNVDAGGSKPSLTNHVAYTAL 540
           +  N  T + L+ G C+ G+I +A EL T  I +  +P C              + YT +
Sbjct: 788 LLANTATYNMLIRGFCRQGRIEEASELITRMIGDGVSPDC--------------ITYTTM 847

Query: 541 IHGLCQDGQFSKAVKLFSDMRRYGLQPDEVIYVVMLRGLFQVKYI--LMMLHADMLKFGV 596
           I+ LC+     KA++L++ M   G++PD V Y  ++ G      +     L  +ML+ G+
Sbjct: 848 INELCRRNDVKKAIELWNSMTEKGIRPDRVAYNTLIHGCCVAGEMGKATELRNEMLRQGL 892

HSP 6 Score: 232.3 bits (591), Expect = 1.5e-59
Identity = 167/631 (26.47%), Postives = 300/631 (47.54%), Query Frame = 1

Query: 11  TPDSLITTVLNCRSPWKALEFFN----AAPEKNIQLYSAIIHVLVGSKLLSHARYLL--- 70
           T  +L+  ++  R    A+E FN         ++ +Y+ +I  L   K LS A+ ++   
Sbjct: 194 TLSALLHGLVKFRHFGLAMELFNDMVSVGIRPDVYIYTGVIRSLCELKDLSRAKEMIAHM 253

Query: 71  ------------NDLVQNLVKSHKPYHACQLAFSELSRLKSSKFTPNV--YGELIIVLCK 130
                       N L+  L K  K + A  +       L      P+V  Y  L+  LCK
Sbjct: 254 EATGCDVNIVPYNVLIDGLCKKQKVWEAVGIK----KDLAGKDLKPDVVTYCTLVYGLCK 313

Query: 131 MELVEEALSMYHKVGA---ALTIQACNVLLYVLVKTGRFELLWRIYEEMISNGLSPSVIT 190
           ++  E  L M  ++     + +  A + L+  L K G+ E    + + ++  G+SP++  
Sbjct: 314 VQEFEIGLEMMDEMLCLRFSPSEAAVSSLVEGLRKRGKIEEALNLVKRVVDFGVSPNLFV 373

Query: 191 FGTLIDGCCRQGDLLRAQEMFDEMRVKGIVPTVIVYTILIRGLCSDNKIEEAESMHRAMR 250
           +  LID  C+      A+ +FD M   G+ P  + Y+ILI   C   K++ A S    M 
Sbjct: 374 YNALIDSLCKGRKFHEAELLFDRMGKIGLRPNDVTYSILIDMFCRRGKLDTALSFLGEMV 433

Query: 251 EVGVYPNVYTYNTLMDGYCKLANAKQALRLYQDMLGEGLVPDVVTFGILIDGLCKFGEMK 310
           + G+  +VY YN+L++G+CK  +   A     +M+ + L P VVT+  L+ G C  G++ 
Sbjct: 434 DTGLKLSVYPYNSLINGHCKFGDISAAEGFMAEMINKKLEPTVVTYTSLMGGYCSKGKIN 493

Query: 311 AARNLFVNMIKFSVTPNIAVYNSLIDAYCKVGDVSEAMALFLELERFEVSPDVFTYSILI 370
            A  L+  M    + P+I  + +L+    + G + +A+ LF E+  + V P+  TY+++I
Sbjct: 494 KALRLYHEMTGKGIAPSIYTFTTLLSGLFRAGLIRDAVKLFNEMAEWNVKPNRVTYNVMI 553

Query: 371 RGLCSVSRTEEAGNIFEKMTKEGILANSVTYNSLIDGCCKEGKMDKALEICSQMTENGVE 430
            G C      +A    ++MT++GI+ ++ +Y  LI G C  G+  +A      + +   E
Sbjct: 554 EGYCEEGDMSKAFEFLKEMTEKGIVPDTYSYRPLIHGLCLTGQASEAKVFVDGLHKGNCE 613

Query: 431 PNVITFSTLIDGYCKIRNLQAAMGIYSEMVIKSLSPDVVTYTAMIDGHCKYGSMKEALKL 490
            N I ++ L+ G+C+   L+ A+ +  EMV + +  D+V Y  +IDG  K+   K    L
Sbjct: 614 LNEICYTGLLHGFCREGKLEEALSVCQEMVQRGVDLDLVCYGVLIDGSLKHKDRKLFFGL 673

Query: 491 YSDMLDNGITPNCYTISCLLDGLCKDGKISDALELFTEKIEFQTPRCNVDAGGSKPSLTN 550
             +M D G+ P+    + ++D   K G   +A  ++   I             ++  + N
Sbjct: 674 LKEMHDRGLKPDDVIYTSMIDAKSKTGDFKEAFGIWDLMI-------------NEGCVPN 733

Query: 551 HVAYTALIHGLCQDGQFSKAVKLFSDMRRYGLQPDEVIYVVMLRGLFQVKYIL---MMLH 610
            V YTA+I+GLC+ G  ++A  L S M+     P++V Y   L  L + +  +   + LH
Sbjct: 734 EVTYTAVINGLCKAGFVNEAEVLCSKMQPVSSVPNQVTYGCFLDILTKGEVDMQKAVELH 793

Query: 611 ADMLKFGVIPNSAVHVILCECYQESGFLKSA 615
             +LK G++ N+A + +L   +   G ++ A
Sbjct: 794 NAILK-GLLANTATYNMLIRGFCRQGRIEEA 806

BLAST of Cucsa.275720 vs. TrEMBL
Match: A0A0A0KS30_CUCSA (Uncharacterized protein OS=Cucumis sativus GN=Csa_5G496480 PE=4 SV=1)

HSP 1 Score: 1229.5 bits (3180), Expect = 0.0e+00
Identity = 616/616 (100.00%), Postives = 616/616 (100.00%), Query Frame = 1

Query: 1   MLQVVSLSSLTPDSLITTVLNCRSPWKALEFFNAAPEKNIQLYSAIIHVLVGSKLLSHAR 60
           MLQVVSLSSLTPDSLITTVLNCRSPWKALEFFNAAPEKNIQLYSAIIHVLVGSKLLSHAR
Sbjct: 32  MLQVVSLSSLTPDSLITTVLNCRSPWKALEFFNAAPEKNIQLYSAIIHVLVGSKLLSHAR 91

Query: 61  YLLNDLVQNLVKSHKPYHACQLAFSELSRLKSSKFTPNVYGELIIVLCKMELVEEALSMY 120
           YLLNDLVQNLVKSHKPYHACQLAFSELSRLKSSKFTPNVYGELIIVLCKMELVEEALSMY
Sbjct: 92  YLLNDLVQNLVKSHKPYHACQLAFSELSRLKSSKFTPNVYGELIIVLCKMELVEEALSMY 151

Query: 121 HKVGAALTIQACNVLLYVLVKTGRFELLWRIYEEMISNGLSPSVITFGTLIDGCCRQGDL 180
           HKVGAALTIQACNVLLYVLVKTGRFELLWRIYEEMISNGLSPSVITFGTLIDGCCRQGDL
Sbjct: 152 HKVGAALTIQACNVLLYVLVKTGRFELLWRIYEEMISNGLSPSVITFGTLIDGCCRQGDL 211

Query: 181 LRAQEMFDEMRVKGIVPTVIVYTILIRGLCSDNKIEEAESMHRAMREVGVYPNVYTYNTL 240
           LRAQEMFDEMRVKGIVPTVIVYTILIRGLCSDNKIEEAESMHRAMREVGVYPNVYTYNTL
Sbjct: 212 LRAQEMFDEMRVKGIVPTVIVYTILIRGLCSDNKIEEAESMHRAMREVGVYPNVYTYNTL 271

Query: 241 MDGYCKLANAKQALRLYQDMLGEGLVPDVVTFGILIDGLCKFGEMKAARNLFVNMIKFSV 300
           MDGYCKLANAKQALRLYQDMLGEGLVPDVVTFGILIDGLCKFGEMKAARNLFVNMIKFSV
Sbjct: 272 MDGYCKLANAKQALRLYQDMLGEGLVPDVVTFGILIDGLCKFGEMKAARNLFVNMIKFSV 331

Query: 301 TPNIAVYNSLIDAYCKVGDVSEAMALFLELERFEVSPDVFTYSILIRGLCSVSRTEEAGN 360
           TPNIAVYNSLIDAYCKVGDVSEAMALFLELERFEVSPDVFTYSILIRGLCSVSRTEEAGN
Sbjct: 332 TPNIAVYNSLIDAYCKVGDVSEAMALFLELERFEVSPDVFTYSILIRGLCSVSRTEEAGN 391

Query: 361 IFEKMTKEGILANSVTYNSLIDGCCKEGKMDKALEICSQMTENGVEPNVITFSTLIDGYC 420
           IFEKMTKEGILANSVTYNSLIDGCCKEGKMDKALEICSQMTENGVEPNVITFSTLIDGYC
Sbjct: 392 IFEKMTKEGILANSVTYNSLIDGCCKEGKMDKALEICSQMTENGVEPNVITFSTLIDGYC 451

Query: 421 KIRNLQAAMGIYSEMVIKSLSPDVVTYTAMIDGHCKYGSMKEALKLYSDMLDNGITPNCY 480
           KIRNLQAAMGIYSEMVIKSLSPDVVTYTAMIDGHCKYGSMKEALKLYSDMLDNGITPNCY
Sbjct: 452 KIRNLQAAMGIYSEMVIKSLSPDVVTYTAMIDGHCKYGSMKEALKLYSDMLDNGITPNCY 511

Query: 481 TISCLLDGLCKDGKISDALELFTEKIEFQTPRCNVDAGGSKPSLTNHVAYTALIHGLCQD 540
           TISCLLDGLCKDGKISDALELFTEKIEFQTPRCNVDAGGSKPSLTNHVAYTALIHGLCQD
Sbjct: 512 TISCLLDGLCKDGKISDALELFTEKIEFQTPRCNVDAGGSKPSLTNHVAYTALIHGLCQD 571

Query: 541 GQFSKAVKLFSDMRRYGLQPDEVIYVVMLRGLFQVKYILMMLHADMLKFGVIPNSAVHVI 600
           GQFSKAVKLFSDMRRYGLQPDEVIYVVMLRGLFQVKYILMMLHADMLKFGVIPNSAVHVI
Sbjct: 572 GQFSKAVKLFSDMRRYGLQPDEVIYVVMLRGLFQVKYILMMLHADMLKFGVIPNSAVHVI 631

Query: 601 LCECYQESGFLKSAQN 617
           LCECYQESGFLKSAQN
Sbjct: 632 LCECYQESGFLKSAQN 647

BLAST of Cucsa.275720 vs. TrEMBL
Match: A5AF05_VITVI (Putative uncharacterized protein OS=Vitis vinifera GN=VITISV_031722 PE=4 SV=1)

HSP 1 Score: 707.2 bits (1824), Expect = 1.7e-200
Identity = 351/616 (56.98%), Postives = 459/616 (74.51%), Query Frame = 1

Query: 6   SLSSLTPDSLITTVLNCRSPWKALEFFNAAPE-----KNIQLYSAIIHVLVGSKLLSHAR 65
           S S  +P SL  ++L CR+  +ALE F++        KN QLYSAIIHVL G+KL + AR
Sbjct: 31  SSSDSSPSSLPNSILTCRTANQALELFHSVSRRADLAKNPQLYSAIIHVLTGAKLYAKAR 90

Query: 66  YLLNDLVQNLVKSHKPYHACQLAFSELSRLKSSKFTPNVYGELIIVLCKMELVEEALSMY 125
            L+ DL+Q L KS +    C + F+ LSRL+SSKFTPNV+G LII   +M LVEEAL +Y
Sbjct: 91  CLMRDLIQCLQKSRRSRICCSV-FNVLSRLESSKFTPNVFGVLIIAFSEMGLVEEALWVY 150

Query: 126 HKVGAALTIQACNVLLYVLVKTGRFELLWRIYEEMISNGLSPSVITFGTLIDGCCRQGDL 185
           +K+     +QACN++L  LVK GRF+ +W++Y +M++ G SP+V+T+GTLIDGCCRQGD 
Sbjct: 151 YKMDVLPAMQACNMVLDGLVKKGRFDTMWKVYGDMVARGASPNVVTYGTLIDGCCRQGDF 210

Query: 186 LRAQEMFDEMRVKGIVPTVIVYTILIRGLCSDNKIEEAESMHRAMREVGVYPNVYTYNTL 245
           L+A  +FDEM  K I PTV++YTILIRGLC +++I EAESM R MR  G+ PN+YTYNT+
Sbjct: 211 LKAFRLFDEMIEKKIFPTVVIYTILIRGLCGESRISEAESMFRTMRNSGMLPNLYTYNTM 270

Query: 246 MDGYCKLANAKQALRLYQDMLGEGLVPDVVTFGILIDGLCKFGEMKAARNLFVNMIKFSV 305
           MDGYCK+A+ K+AL LY +MLG+GL+P+VVTFGILIDGLCK  EM +AR   ++M  F V
Sbjct: 271 MDGYCKIAHVKKALELYXEMLGDGLLPNVVTFGILIDGLCKTDEMVSARKFLIDMASFGV 330

Query: 306 TPNIAVYNSLIDAYCKVGDVSEAMALFLELERFEVSPDVFTYSILIRGLCSVSRTEEAGN 365
            PNI VYN LID YCK G++SEA++L  E+E+ E+ PDVFTYSILI+GLC V R EEA  
Sbjct: 331 VPNIFVYNCLIDGYCKAGNLSEALSLHSEIEKHEILPDVFTYSILIKGLCGVDRMEEADG 390

Query: 366 IFEKMTKEGILANSVTYNSLIDGCCKEGKMDKALEICSQMTENGVEPNVITFSTLIDGYC 425
           + ++M K+G L N+VTYN+LIDG CKEG M+KA+E+CSQMTE G+EPN+ITFSTLIDGYC
Sbjct: 391 LLQEMKKKGFLPNAVTYNTLIDGYCKEGNMEKAIEVCSQMTEKGIEPNIITFSTLIDGYC 450

Query: 426 KIRNLQAAMGIYSEMVIKSLSPDVVTYTAMIDGHCKYGSMKEALKLYSDMLDNGITPNCY 485
           K   ++AAMG+Y+EMVIK L PDVV YTA+IDGH K G+ KEA +L+ +M + G+ PN +
Sbjct: 451 KAGKMEAAMGLYTEMVIKGLLPDVVAYTALIDGHFKDGNTKEAFRLHKEMQEAGLHPNVF 510

Query: 486 TISCLLDGLCKDGKISDALELFTEKIEFQTPRCNVDAGGSKPSLTNHVAYTALIHGLCQD 545
           T+SCL+DGLCKDG+ISDA++LF  K    T     +         NHV YTALI GLC D
Sbjct: 511 TLSCLIDGLCKDGRISDAIKLFLAKTGTDTTGSKTNELDRSLCSPNHVMYTALIQGLCTD 570

Query: 546 GQFSKAVKLFSDMRRYGLQPDEVIYVVMLRGLFQVKYI--LMMLHADMLKFGVIPNSAVH 605
           G+  KA K FSDMR  GL+PD    +V+++G F+  ++  +MML AD+LK G+IPNS+V+
Sbjct: 571 GRIFKASKFFSDMRCSGLRPDVFTCIVIIQGHFRAMHLRDVMMLQADILKMGIIPNSSVY 630

Query: 606 VILCECYQESGFLKSA 615
            +L + Y+ESG+LKSA
Sbjct: 631 RVLAKGYEESGYLKSA 645

BLAST of Cucsa.275720 vs. TrEMBL
Match: A5AF05_VITVI (Putative uncharacterized protein OS=Vitis vinifera GN=VITISV_031722 PE=4 SV=1)

HSP 1 Score: 114.0 bits (284), Expect = 6.5e-22
Identity = 130/593 (21.92%), Postives = 248/593 (41.82%), Query Frame = 1

Query: 43  YSAIIHVLVGSKLLSHARYLLNDLVQN-----------LVKSHKPYHACQLAFSELSRLK 102
           YS +I  L G   +  A  LL ++ +            L+  +      + A    S++ 
Sbjct: 371 YSILIKGLCGVDRMEEADGLLQEMKKKGFLPNAVTYNTLIDGYCKEGNMEKAIEVCSQMT 430

Query: 103 SSKFTPNV--YGELIIVLCKMELVEEALSMYHKV---GAALTIQACNVLLYVLVKTGRFE 162
                PN+  +  LI   CK   +E A+ +Y ++   G    + A   L+    K G  +
Sbjct: 431 EKGIEPNIITFSTLIDGYCKAGKMEAAMGLYTEMVIKGLLPDVVAYTALIDGHFKDGNTK 490

Query: 163 LLWRIYEEMISNGLSPSVITFGTLIDGCCRQGDLLRAQEMF-------------DEMRVK 222
             +R+++EM   GL P+V T   LIDG C+ G +  A ++F             +E+   
Sbjct: 491 EAFRLHKEMQEAGLHPNVFTLSCLIDGLCKDGRISDAIKLFLAKTGTDTTGSKTNELDRS 550

Query: 223 GIVPTVIVYTILIRGLCSDNKIEEAESMHRAMREVGVYPNVYTYNTLMDGYCKLANAKQA 282
              P  ++YT LI+GLC+D +I +A      MR  G+ P+V+T   ++ G+ +  + +  
Sbjct: 551 LCSPNHVMYTALIQGLCTDGRIFKASKFFSDMRCSGLRPDVFTCIVIIQGHFRAMHLRDV 610

Query: 283 LRLYQDMLGEGLVPDVVTFGILIDGLCKFGEMKAARNLFVNMIKFSVTPNIAVYNSLIDA 342
           + L  D+L  G++P+   + +L  G  + G +K+A    ++     V P   V +    +
Sbjct: 611 MMLQADILKMGIIPNSSVYRVLAKGYEESGYLKSA----LSFCGEGVQPLDRVNSEPWGS 670

Query: 343 YCKVGDVSEAMALFLELERFEVSPDVFT-----YSILIRGLCSVSRTEEAGNIFEKMTKE 402
           Y     +     L +  E+ E      T       +L+    ++ +      I +K+   
Sbjct: 671 Y----TIRSRFQLCVVTEKKECHSSYLTAFGIHSFVLLWYFHTILKPPSPHEILKKVPFI 730

Query: 403 GILANSVTYNSLIDGCCKEGKMDKALEICSQMTENGVEPNVITFSTLIDGYCKIRNLQAA 462
           G   +               + +KAL++  QM  +  +P+++T +  +     +  L   
Sbjct: 731 GQKGDQ--------------RPNKALQLFRQMQMDDXQPDIVTVTVALSACADLGALDMG 790

Query: 463 MGIYSEMVIKSLSPDVVTYTAMIDGHCKYGSMKEALKLYSDMLDNGITPNCYTISCLLDG 522
             I++ +  + L  D+    ++I+ + K G +  A +L+       +T    T + ++ G
Sbjct: 791 EWIHAYIRHRGLDTDLCLNNSLINMYSKCGEIGTARRLFDGTQKKDVT----TWTSMIVG 850

Query: 523 LCKDGKISDALELFTEKIEF-QTPRCNVDAGGSKPSLT--NHVAYTALIHGLCQDGQFSK 582
               G+  +AL+LFTE  E  +  R N   G  + SL   N V +  ++      G   +
Sbjct: 851 HALHGQAEEALQLFTEMKETNKRARKNKRNGEXESSLVLPNDVTFMGVLMACSHAGLVEE 910

Query: 583 AVKLFSDMRR-YGLQPDEVIYVVMLRGLFQVKYILMMLHADMLKFGVIPNSAV 598
             + F  M+  Y L+P  + +   +  L     +L   +  +LK  V PN+ V
Sbjct: 911 GKQHFRSMKEDYSLRP-RISHFGCMVDLLCRAGLLTEAYEFILKMPVRPNAVV 936

HSP 2 Score: 684.1 bits (1764), Expect = 1.6e-193
Identity = 334/617 (54.13%), Postives = 452/617 (73.26%), Query Frame = 1

Query: 8   SSLTPDSLITTVLNCRSPWKALEFFNAA-------PEKNIQLYSAIIHVLVGSKLLSHAR 67
           SS +   L T +L+  +P +AL+FF          P KN+ LYSA+IHVL  +++ + AR
Sbjct: 26  SSRSSSDLTTAILDSETPEQALQFFTNVLNQNPKNPTKNLHLYSAVIHVLTSARIYTTAR 85

Query: 68  YLLNDLVQNLVKSHKPYHACQLAFSELSRLKSSKFTPNVYGELIIVLCKMELVEEALSMY 127
            L  DL+Q L++S KPY    L F+ L++L+  KF+PNV+G LII   ++ L++EALS+Y
Sbjct: 86  CLTKDLIQTLLQSRKPYRISSLVFNALNQLQGPKFSPNVFGVLIIAFSELGLLDEALSVY 145

Query: 128 HKVGAALTIQACNVLLYVLVKTGRFELLWRIYEEMISNGLSPSVITFGTLIDGCCRQGDL 187
            K G    +QACN LL  LVK G F+ LW +Y++M+S GL PSV+T+  L+D CC QGD+
Sbjct: 146 RKTGIFPAVQACNALLNGLVKKGSFDSLWELYKDMVSRGLVPSVVTYNVLVDACCSQGDI 205

Query: 188 LRAQEMFDEMRVKGIVPTVIVYTILIRGLCSDNKIEEAESMHRAMREVGVYPNVYTYNTL 247
            +A+ + +EM  KGI PTV++Y+ L+RGLCS++K+ EA+ M R M+E GV PN+YTYN L
Sbjct: 206 WKAKSLINEMEKKGIEPTVVIYSTLMRGLCSESKLTEAQDMLRQMKESGVLPNLYTYNVL 265

Query: 248 MDGYCKLANAKQALRLYQDMLGEGLVPDVVTFGILIDGLCKFGEMKAARNLFVNMIKFSV 307
           MDGYCK+A  KQ L L+QD+L +GL P+VVTFGIL+D LCK G++ AARNLFV M K  V
Sbjct: 266 MDGYCKIAKIKQVLDLFQDLLNDGLQPNVVTFGILVDALCKVGKLLAARNLFVQMAKLGV 325

Query: 308 TPNIAVYNSLIDAYCKVGDVSEAMALFLELERFEVSPDVFTYSILIRGLCSVSRTEEAGN 367
            PN+ VYNSLI+ Y K G++ +AM L LE+E+F++ PDVFTYSILI+ +CS+S  +EA  
Sbjct: 326 VPNVLVYNSLINGYSKAGNLPKAMDLLLEMEKFKIVPDVFTYSILIKSVCSLSTVKEADR 385

Query: 368 IFEKMTKEGILANSVTYNSLIDGCCKEGKMDKALEICSQMTENGVEPNVITFSTLIDGYC 427
           I +KM KEG+ ANSV YNS+IDG CK+G M+KALE+C++MT+ GVEPNVITFSTLIDGYC
Sbjct: 386 ILKKMEKEGVPANSVIYNSMIDGYCKKGNMEKALEVCAEMTKKGVEPNVITFSTLIDGYC 445

Query: 428 KIRNLQAAMGIYSEMVIKSLSPDVVTYTAMIDGHCKYGSMKEALKLYSDM-LDNGITPNC 487
           K  N+Q+AMG+YSEM+IKSL PDVV +TA+IDGHCK G+MKEAL+LY  M  D G++PN 
Sbjct: 446 KEGNMQSAMGLYSEMLIKSLVPDVVAFTALIDGHCKSGNMKEALRLYKHMQQDAGLSPNV 505

Query: 488 YTISCLLDGLCKDGKISDALELFTEKIEFQTPRCNVDAGGSKPSLTNHVAYTALIHGLCQ 547
           +T S L+DGLCK G++SDAL+LF +K      R  ++   S+    N+V YT+LI  LC+
Sbjct: 506 FTFSSLIDGLCKAGRVSDALKLFLDKTRGYCSRNKINGTDSRLYSPNYVIYTSLIQALCK 565

Query: 548 DGQFSKAVKLFSDMRRYGLQPDEVIYVVMLRGLFQVKYIL--MMLHADMLKFGVIPNSAV 607
           +GQ  KA KLF DMR   L+PD + Y V+L+G   VK+++  M+LHADM+K G++PN  +
Sbjct: 566 EGQMFKASKLFFDMRCNDLRPDALAYTVILQGHLNVKHVIDVMILHADMIKMGIVPNEVI 625

Query: 608 HVILCECYQESGFLKSA 615
           + IL   Y+ESG+LKSA
Sbjct: 626 YRILMRGYRESGYLKSA 642

BLAST of Cucsa.275720 vs. TrEMBL
Match: V4TQ94_9ROSI (Uncharacterized protein OS=Citrus clementina GN=CICLE_v10024595mg PE=4 SV=1)

HSP 1 Score: 662.1 bits (1707), Expect = 6.4e-187
Identity = 338/617 (54.78%), Postives = 441/617 (71.47%), Query Frame = 1

Query: 6   SLSSLTPDSLITT-VLNCRSPWKALEFFNAA-----PEKNIQLYSAIIHVLVGSKLLSHA 65
           S SSL P S +T  +LN ++P +AL  FN++     P K++  ++AI +VL  +KL  +A
Sbjct: 47  SSSSLPPRSNLTNAILNSKTPNQALVLFNSSSKKLNPTKSLAPFAAIFYVLANAKLYKNA 106

Query: 66  RYLLNDLVQNLVKSHKPYHACQLAFSELSRLKSSKFTPNVYGELIIVLCKMELVEEALSM 125
           R L+ D+ +NL+KS KP+H C   F+ L+ L+  KF P+V+  LII   +M  +EEAL +
Sbjct: 107 RCLIKDVTENLLKSRKPHHVCYSVFNALNSLEIPKFNPSVFSTLIIAFSEMGHIEEALWV 166

Query: 126 YHKVGAALTIQACNVLLYVLVKTGRFELLWRIYEEMISNGLSPSVITFGTLIDGCCRQGD 185
           Y K+     IQACN LL  L+K G+F+ +W  YEEM+  GL   V+T+G LID CC QGD
Sbjct: 167 YRKIEVLPAIQACNALLNGLIKKGKFDSVWEFYEEMVLCGLVADVVTYGVLIDCCCGQGD 226

Query: 186 LLRAQEMFDEMRVKGIVPTVIVYTILIRGLCSDNKIEEAESMHRAMREVGVYPNVYTYNT 245
           +++A  +FDEM  KGI PTV++YTILI GLC++NK+ EAESM R+MRE GV PN+YTYN 
Sbjct: 227 VMKALNLFDEMIDKGIEPTVVIYTILIHGLCNENKMVEAESMFRSMRECGVVPNLYTYNA 286

Query: 246 LMDGYCKLANAKQALRLYQDMLGEGLVPDVVTFGILIDGLCKFGEMKAARNLFVNMIKFS 305
           LMDGYCK+A+  +AL  Y +ML   L P+VVTFG+L+DGLCK GE++AA N FV+M KF 
Sbjct: 287 LMDGYCKVADVNRALEFYHEMLHHNLQPNVVTFGVLMDGLCKVGELRAAGNFFVHMAKFG 346

Query: 306 VTPNIAVYNSLIDAYCKVGDVSEAMALFLELERFEVSPDVFTYSILIRGLCSVSRTEEAG 365
           V PNI VYN LID +CK G++ EAM+L  E+E+FE+SPDVFTY+ILI+GLC V + E A 
Sbjct: 347 VFPNIFVYNCLIDGHCKAGNLFEAMSLCSEMEKFEISPDVFTYNILIKGLCGVGQLEGAE 406

Query: 366 NIFEKMTKEGILANSVTYNSLIDGCCKEGKMDKALEICSQMTENGVEPNVITFSTLIDGY 425
            + +KM KEGILAN VTYNSLIDG CKEG M+KAL +CSQMTE GVEPNV+TFS+LIDG 
Sbjct: 407 GLLQKMYKEGILANVVTYNSLIDGYCKEGDMEKALSVCSQMTEKGVEPNVVTFSSLIDGQ 466

Query: 426 CKIRNLQAAMGIYSEMVIKSLSPDVVTYTAMIDGHCKYGSMKEALKLYSDMLDNGITPNC 485
           CK  N+ AAMG+Y+EMVIKSL PDVV +TA+IDG  K G+MKE L+LY +ML+  ITP+ 
Sbjct: 467 CKAGNIDAAMGLYTEMVIKSLVPDVVVFTALIDGLSKDGNMKETLRLYKEMLEAKITPSV 526

Query: 486 YTISCLLDGLCKDGKISDALELFTEKIEFQTPRCNVDAGGSKPSLTNHVAYTALIHGLCQ 545
           +T+S L+ GL K+G+IS+AL  F EK +        D G   P   NHV Y A+I  LC 
Sbjct: 527 FTVSSLIHGLFKNGRISNALNFFLEKTD------KTDGGYCSP---NHVLYAAIIQALCY 586

Query: 546 DGQFSKAVKLFSDMRRYGLQPDEVIYVVMLRGLFQVKYIL--MMLHADMLKFGVIPNSAV 605
           DGQ  KA KLFSDMR   L+PD   Y  MLRGL + K +L  MML ADM+K G++P++ +
Sbjct: 587 DGQILKASKLFSDMRSDNLRPDNCTYTTMLRGLLRAKRMLDVMMLLADMIKMGIVPDAVI 646

Query: 606 HVILCECYQESGFLKSA 615
           + ++   YQE+G LKSA
Sbjct: 647 NQVMVRGYQENGDLKSA 654

BLAST of Cucsa.275720 vs. TrEMBL
Match: A0A061G4F9_THECC (Pentatricopeptide repeat-containing protein, putative OS=Theobroma cacao GN=TCM_014098 PE=4 SV=1)

HSP 1 Score: 655.2 bits (1689), Expect = 7.8e-185
Identity = 330/611 (54.01%), Postives = 439/611 (71.85%), Query Frame = 1

Query: 14  SLITTVLNCRSPWKALEFFNAA-----PEKNIQLYSAIIHVLVGSKLLSHARYLLNDLVQ 73
           +L   +LN ++P +AL  FN+      P KN++ YSAIIHVL G+KL + AR L+  L++
Sbjct: 35  NLTKAILNSQTPHQALNLFNSNIKLINPSKNLEPYSAIIHVLTGAKLYTDARCLIKYLIK 94

Query: 74  NLVKSHKPYHACQLAFSELSRLKSSKFTPNVYGELIIVLCKMELVEEALSMYHKVGAALT 133
            L  S KP  AC L F+ LS+L++SKFTPNV+G LII   +M L+EEAL +Y K+     
Sbjct: 95  TLQSSLKPRRACHLIFNALSKLQTSKFTPNVFGSLIIAFSEMGLIEEALWVYRKIRTFPP 154

Query: 134 IQACNVLLYVLVKTGRFELLWRIYEEMISNGLSPSVITFGTLIDGCCRQGDLLRAQEMFD 193
           +QACN LL  LVK GRF+ +W +Y +++S G  P+V+T+G LI+GCC QGD  +A+E+F 
Sbjct: 155 MQACNSLLDGLVKMGRFDSMWDVYYDLLSRGFLPNVVTYGVLINGCCCQGDASKARELFH 214

Query: 194 EMRVKGIVPTVIVYTILIRGLCSDNKIEEAESMHRAMREVGVYPNVYTYNTLMDGYCKLA 253
           E+ +KGI P V+++T +I+ LCS+ ++ EAE M R ++++   PN+YT+N LM+GYCK+ 
Sbjct: 215 ELLMKGIQPNVVIFTTVIKILCSEGQMLEAECMFRLIKDLYFLPNLYTFNVLMNGYCKMD 274

Query: 254 NAKQALRLYQDMLGEGLVPDVVTFGILIDGLCKFGEMKAARNLFVNMIKFSVTPNIAVYN 313
           N ++A  +Y  M+G+GL P+VVTFGILIDGLCK G +  ARN FV M+K+ V PN+ VYN
Sbjct: 275 NVERAFEIYWMMIGDGLRPNVVTFGILIDGLCKMGALVVARNYFVCMVKYGVFPNVFVYN 334

Query: 314 SLIDAYCKVGDVSEAMALFLELERFEVSPDVFTYSILIRGLCSVSRTEEAGNIFEKMTKE 373
            LID YCK G+VSEA+ L  E+E+ ++ PDVFTYSILI+GLCSV R EE   + +KM K+
Sbjct: 335 CLIDGYCKAGNVSEAVELSSEMEKLKILPDVFTYSILIKGLCSVGRVEEGSFLLQKMIKD 394

Query: 374 GILANSVTYNSLIDGCCKEGKMDKALEICSQMTENGVEPNVITFSTLIDGYCKIRNLQAA 433
           G+LANSVTYNSLIDG C+ G M+KALEICSQMTE GVEPNVITFSTLIDGYCK  N+QAA
Sbjct: 395 GVLANSVTYNSLIDGYCRVGNMEKALEICSQMTEKGVEPNVITFSTLIDGYCKAGNMQAA 454

Query: 434 MGIYSEMVIKSLSPDVVTYTAMIDGHCKYGSMKEALKLYSDMLDNGITPNCYTISCLLDG 493
           MG YSEMVIKS+ PDVV YTA+I+G CK G++KEAL+L+  ML +G+TPN +T+SCL+DG
Sbjct: 455 MGFYSEMVIKSIVPDVVAYTALINGCCKNGNVKEALRLHKVMLGSGLTPNAFTLSCLVDG 514

Query: 494 LCKDGKISDALELFTEKIEFQTPRCNVDAGGSKPSLTNHV---AYTALIHGLCQDGQFSK 553
           LCKDG + +A  +F EK         ++       L NHV    YT LI  LC+DGQ  K
Sbjct: 515 LCKDGIVFEAFSVFLEKTRAGISENGINEMDGLFCLPNHVMYMIYTTLIQALCKDGQIFK 574

Query: 554 AVKLFSDMRRYGLQPDEVIYVVMLRGLFQVKYIL--MMLHADMLKFGVIPNSAVHVILCE 613
           A K+FSD+R   L  D   Y+VML G FQ K ++  MMLHADM+K G++P+  V++I+  
Sbjct: 575 ANKIFSDIRCIDLIADVPSYIVMLEGHFQAKNMIDVMMLHADMIKIGIMPSITVNMIMAR 634

Query: 614 CYQESGFLKSA 615
            YQE G L+ A
Sbjct: 635 GYQEIGDLRLA 645

BLAST of Cucsa.275720 vs. TAIR10
Match: AT5G61400.1 (AT5G61400.1 Pentatricopeptide repeat (PPR) superfamily protein)

HSP 1 Score: 537.3 bits (1383), Expect = 1.2e-152
Identity = 282/617 (45.71%), Postives = 393/617 (63.70%), Query Frame = 1

Query: 6   SLSSLTPDSLITTVLNCRSPWKALEFFNAAPEKNI------QLYSAIIHVLVGSKLLSHA 65
           S SS +  SL   +L CRS  +A + F  +    +      Q +SA+IHVL G+   + A
Sbjct: 35  SASSFSSSSLAEAILKCRSAEEAFKLFETSSRSRVSKSNDLQSFSAVIHVLTGAHKYTLA 94

Query: 66  RYLLNDLVQNLVKSHKPYHACQLAFSELSRLKSSKFTPNVYGELIIVLCKMELVEEALSM 125
           R L+  L++ L +  +P +     F+ L  ++S KF+  V+  LI+   +M L EEAL +
Sbjct: 95  RCLIKSLIERLKRHSEPSNMSHRLFNALEDIQSPKFSIGVFSLLIMEFLEMGLFEEALWV 154

Query: 126 YHKVGAALTIQACNVLLYVLVKTGRFELLWRIYEEMISNGLSPSVITFGTLIDGCCRQGD 185
             ++  +   +AC  +L  LV+  RF+ +W  Y+ MIS GL P V  +  L   C +QG 
Sbjct: 155 SREMKCSPDSKACLSILNGLVRRRRFDSVWVDYQLMISRGLVPDVHIYFVLFQCCFKQGL 214

Query: 186 LLRAQEMFDEMRVKGIVPTVIVYTILIRGLCSDNKIEEAESMHRAMREVGVYPNVYTYNT 245
             + +++ DEM   GI P V +YTI I  LC DNK+EEAE M   M++ GV PN+YTY+ 
Sbjct: 215 YSKKEKLLDEMTSLGIKPNVYIYTIYILDLCRDNKMEEAEKMFELMKKHGVLPNLYTYSA 274

Query: 246 LMDGYCKLANAKQALRLYQDMLGEGLVPDVVTFGILIDGLCKFGEMKAARNLFVNMIKFS 305
           ++DGYCK  N +QA  LY+++L   L+P+VV FG L+DG CK  E+  AR+LFV+M+KF 
Sbjct: 275 MIDGYCKTGNVRQAYGLYKEILVAELLPNVVVFGTLVDGFCKARELVTARSLFVHMVKFG 334

Query: 306 VTPNIAVYNSLIDAYCKVGDVSEAMALFLELERFEVSPDVFTYSILIRGLCSVSRTEEAG 365
           V PN+ VYN LI  +CK G++ EA+ L  E+E   +SPDVFTY+ILI GLC   +  EA 
Sbjct: 335 VDPNLYVYNCLIHGHCKSGNMLEAVGLLSEMESLNLSPDVFTYTILINGLCIEDQVAEAN 394

Query: 366 NIFEKMTKEGILANSVTYNSLIDGCCKEGKMDKALEICSQMTENGVEPNVITFSTLIDGY 425
            +F+KM  E I  +S TYNSLI G CKE  M++AL++CS+MT +GVEPN+ITFSTLIDGY
Sbjct: 395 RLFQKMKNERIFPSSATYNSLIHGYCKEYNMEQALDLCSEMTASGVEPNIITFSTLIDGY 454

Query: 426 CKIRNLQAAMGIYSEMVIKSLSPDVVTYTAMIDGHCKYGSMKEALKLYSDMLDNGITPNC 485
           C +R+++AAMG+Y EM IK + PDVVTYTA+ID H K  +MKEAL+LYSDML+ GI PN 
Sbjct: 455 CNVRDIKAAMGLYFEMTIKGIVPDVVTYTALIDAHFKEANMKEALRLYSDMLEAGIHPND 514

Query: 486 YTISCLLDGLCKDGKISDALELFTEKIEFQTPRCNVDAGGSKPSLTNHVAYTALIHGLCQ 545
           +T +CL+DG  K+G++S A++ + E                + S  NHV +T LI GLCQ
Sbjct: 515 HTFACLVDGFWKEGRLSVAIDFYQE-------------NNQQRSCWNHVGFTCLIEGLCQ 574

Query: 546 DGQFSKAVKLFSDMRRYGLQPDEVIYVVMLRGLFQVKYI--LMMLHADMLKFGVIPNSAV 605
           +G   +A + FSDMR  G+ PD   YV ML+G  Q K I   MML  DM+K G++PN  V
Sbjct: 575 NGYILRASRFFSDMRSCGITPDICSYVSMLKGHLQEKRITDTMMLQCDMIKTGILPNLLV 634

Query: 606 HVILCECYQESGFLKSA 615
           + +L   YQ +G++KSA
Sbjct: 635 NQLLARFYQANGYVKSA 638

BLAST of Cucsa.275720 vs. TAIR10
Match: AT2G02150.1 (AT2G02150.1 Tetratricopeptide repeat (TPR)-like superfamily protein)

HSP 1 Score: 296.6 bits (758), Expect = 3.6e-80
Identity = 174/566 (30.74%), Postives = 296/566 (52.30%), Query Frame = 1

Query: 25  PWKALEFFNAAPEKN-----IQLYSAIIHVLVGSKLLSHARYLLNDLVQNLVKSHKPYHA 84
           P  A +FF  +  +N     ++ Y  + H+L  +++     Y  N +++ +V S      
Sbjct: 122 PKLAFKFFKWSMTRNGFKHSVESYCIVAHILFCARMY----YDANSVLKEMVLSKADCDV 181

Query: 85  CQLAFSELSRLKSSKFTPNVYGELIIVLCKMELVEEALSMYHKVGAALTI---QACNVLL 144
             + +S  +          V+  L  VL  + ++EEA+  + K+         ++CN LL
Sbjct: 182 FDVLWSTRNVCVPGF---GVFDALFSVLIDLGMLEEAIQCFSKMKRFRVFPKTRSCNGLL 241

Query: 145 YVLVKTGRFELLWRIYEEMISNGLSPSVITFGTLIDGCCRQGDLLRAQEMFDEMRVKGIV 204
           +   K G+ + + R +++MI  G  P+V T+  +ID  C++GD+  A+ +F+EM+ +G+V
Sbjct: 242 HRFAKLGKTDDVKRFFKDMIGAGARPTVFTYNIMIDCMCKEGDVEAARGLFEEMKFRGLV 301

Query: 205 PTVIVYTILIRGLCSDNKIEEAESMHRAMREVGVYPNVYTYNTLMDGYCKLANAKQALRL 264
           P  + Y  +I G     ++++       M+++   P+V TYN L++ +CK       L  
Sbjct: 302 PDTVTYNSMIDGFGKVGRLDDTVCFFEEMKDMCCEPDVITYNALINCFCKFGKLPIGLEF 361

Query: 265 YQDMLGEGLVPDVVTFGILIDGLCKFGEMKAARNLFVNMIKFSVTPNIAVYNSLIDAYCK 324
           Y++M G GL P+VV++  L+D  CK G M+ A   +V+M +  + PN   Y SLIDA CK
Sbjct: 362 YREMKGNGLKPNVVSYSTLVDAFCKEGMMQQAIKFYVDMRRVGLVPNEYTYTSLIDANCK 421

Query: 325 VGDVSEAMALFLELERFEVSPDVFTYSILIRGLCSVSRTEEAGNIFEKMTKEGILANSVT 384
           +G++S+A  L  E+ +  V  +V TY+ LI GLC   R +EA  +F KM   G++ N  +
Sbjct: 422 IGNLSDAFRLGNEMLQVGVEWNVVTYTALIDGLCDAERMKEAEELFGKMDTAGVIPNLAS 481

Query: 385 YNSLIDGCCKEGKMDKALEICSQMTENGVEPNVITFSTLIDGYCKIRNLQAAMGIYSEMV 444
           YN+LI G  K   MD+ALE+ +++   G++P+++ + T I G C +  ++AA  + +EM 
Sbjct: 482 YNALIHGFVKAKNMDRALELLNELKGRGIKPDLLLYGTFIWGLCSLEKIEAAKVVMNEMK 541

Query: 445 IKSLSPDVVTYTAMIDGHCKYGSMKEALKLYSDMLDNGITPNCYTISCLLDGLCKDGKIS 504
              +  + + YT ++D + K G+  E L L  +M +  I     T   L+DGLCK+  +S
Sbjct: 542 ECGIKANSLIYTTLMDAYFKSGNPTEGLHLLDEMKELDIEVTVVTFCVLIDGLCKNKLVS 601

Query: 505 DALELFTEKIEFQTPRCNVDAGGSKPSLTNHVAYTALIHGLCQDGQFSKAVKLFSDMRRY 564
            A++ F         R + D G       N   +TA+I GLC+D Q   A  LF  M + 
Sbjct: 602 KAVDYFN--------RISNDFG----LQANAAIFTAMIDGLCKDNQVEAATTLFEQMVQK 661

Query: 565 GLQPDEVIYVVMLRGLFQVKYILMML 583
           GL PD   Y  ++ G F+   +L  L
Sbjct: 662 GLVPDRTAYTSLMDGNFKQGNVLEAL 668

HSP 2 Score: 258.1 bits (658), Expect = 1.4e-68
Identity = 159/480 (33.12%), Postives = 244/480 (50.83%), Query Frame = 1

Query: 100 YGELIIVLCKMELVEEALSMYHKV---GAALTIQACNVLLYVLVKTGRFELLWRIYEEMI 159
           Y  +I  +CK   VE A  ++ ++   G        N ++    K GR +     +EEM 
Sbjct: 265 YNIMIDCMCKEGDVEAARGLFEEMKFRGLVPDTVTYNSMIDGFGKVGRLDDTVCFFEEMK 324

Query: 160 SNGLSPSVITFGTLIDGCCRQGDLLRAQEMFDEMRVKGIVPTVIVYTILIRGLCSDNKIE 219
                P VIT+  LI+  C+ G L    E + EM+  G+ P V+ Y+ L+   C +  ++
Sbjct: 325 DMCCEPDVITYNALINCFCKFGKLPIGLEFYREMKGNGLKPNVVSYSTLVDAFCKEGMMQ 384

Query: 220 EAESMHRAMREVGVYPNVYTYNTLMDGYCKLANAKQALRLYQDMLGEGLVPDVVTFGILI 279
           +A   +  MR VG+ PN YTY +L+D  CK+ N   A RL  +ML  G+  +VVT+  LI
Sbjct: 385 QAIKFYVDMRRVGLVPNEYTYTSLIDANCKIGNLSDAFRLGNEMLQVGVEWNVVTYTALI 444

Query: 280 DGLCKFGEMKAARNLFVNMIKFSVTPNIAVYNSLIDAYCKVGDVSEAMALFLELERFEVS 339
           DGLC    MK A  LF  M    V PN+A YN+LI  + K  ++  A+ L  EL+   + 
Sbjct: 445 DGLCDAERMKEAEELFGKMDTAGVIPNLASYNALIHGFVKAKNMDRALELLNELKGRGIK 504

Query: 340 PDVFTYSILIRGLCSVSRTEEAGNIFEKMTKEGILANSVTYNSLIDGCCKEGKMDKALEI 399
           PD+  Y   I GLCS+ + E A  +  +M + GI ANS+ Y +L+D   K G   + L +
Sbjct: 505 PDLLLYGTFIWGLCSLEKIEAAKVVMNEMKECGIKANSLIYTTLMDAYFKSGNPTEGLHL 564

Query: 400 CSQMTENGVEPNVITFSTLIDGYCKIRNLQAAMGIYSEMVIK-SLSPDVVTYTAMIDGHC 459
             +M E  +E  V+TF  LIDG CK + +  A+  ++ +     L  +   +TAMIDG C
Sbjct: 565 LDEMKELDIEVTVVTFCVLIDGLCKNKLVSKAVDYFNRISNDFGLQANAAIFTAMIDGLC 624

Query: 460 KYGSMKEALKLYSDMLDNGITPNCYTISCLLDGLCKDGKISDALELFTEKIEFQTPRCNV 519
           K   ++ A  L+  M+  G+ P+    + L+DG  K G + +AL L  +  E        
Sbjct: 625 KDNQVEAATTLFEQMVQKGLVPDRTAYTSLMDGNFKQGNVLEALALRDKMAEI------- 684

Query: 520 DAGGSKPSLTNHVAYTALIHGLCQDGQFSKAVKLFSDMRRYGLQPDEVIYVVMLRGLFQV 576
              G K  L   +AYT+L+ GL    Q  KA     +M   G+ PDEV+ + +L+  +++
Sbjct: 685 ---GMKLDL---LAYTSLVWGLSHCNQLQKARSFLEEMIGEGIHPDEVLCISVLKKHYEL 731

HSP 3 Score: 226.5 bits (576), Expect = 4.5e-59
Identity = 150/536 (27.99%), Postives = 257/536 (47.95%), Query Frame = 1

Query: 84  FSELSRLKSSKFTPNVYGEL--IIVLCKMELVEEALSMYHKVGAALTIQACNVLLYVLVK 143
           FS++ R +    T +  G L     L K + V+         GA  T+   N+++  + K
Sbjct: 215 FSKMKRFRVFPKTRSCNGLLHRFAKLGKTDDVKRFFKDMIGAGARPTVFTYNIMIDCMCK 274

Query: 144 TGRFELLWRIYEEMISNGLSPSVITFGTLIDGCCRQGDLLRAQEMFDEMRVKGIVPTVIV 203
            G  E    ++EEM   GL P  +T+ ++IDG  + G L      F+EM+     P VI 
Sbjct: 275 EGDVEAARGLFEEMKFRGLVPDTVTYNSMIDGFGKVGRLDDTVCFFEEMKDMCCEPDVIT 334

Query: 204 YTILIRGLCSDNKIEEAESMHRAMREVGVYPNVYTYNTLMDGYCKLANAKQALRLYQDML 263
           Y  LI   C   K+      +R M+  G+ PNV +Y+TL+D +CK    +QA++ Y DM 
Sbjct: 335 YNALINCFCKFGKLPIGLEFYREMKGNGLKPNVVSYSTLVDAFCKEGMMQQAIKFYVDMR 394

Query: 264 GEGLVPDVVTFGILIDGLCKFGEMKAARNLFVNMIKFSVTPNIAVYNSLIDAYCKVGDVS 323
             GLVP+  T+  LID  CK G +  A  L   M++  V  N+  Y +LID  C    + 
Sbjct: 395 RVGLVPNEYTYTSLIDANCKIGNLSDAFRLGNEMLQVGVEWNVVTYTALIDGLCDAERMK 454

Query: 324 EAMALFLELERFEVSPDVFTYSILIRGLCSVSRTEEAGNIFEKMTKEGILANSVTYNSLI 383
           EA  LF +++   V P++ +Y+ LI G       + A  +  ++   GI  + + Y + I
Sbjct: 455 EAEELFGKMDTAGVIPNLASYNALIHGFVKAKNMDRALELLNELKGRGIKPDLLLYGTFI 514

Query: 384 DGCCKEGKMDKALEICSQMTENGVEPNVITFSTLIDGYCKIRNLQAAMGIYSEMVIKSLS 443
            G C   K++ A  + ++M E G++ N + ++TL+D Y K  N    + +  EM    + 
Sbjct: 515 WGLCSLEKIEAAKVVMNEMKECGIKANSLIYTTLMDAYFKSGNPTEGLHLLDEMKELDIE 574

Query: 444 PDVVTYTAMIDGHCKYGSMKEALKLYSDML-DNGITPNCYTISCLLDGLCKDGKISDALE 503
             VVT+  +IDG CK   + +A+  ++ +  D G+  N    + ++DGLCKD ++  A  
Sbjct: 575 VTVVTFCVLIDGLCKNKLVSKAVDYFNRISNDFGLQANAAIFTAMIDGLCKDNQVEAATT 634

Query: 504 LFTEKIEFQTPRCNVDAGGSKPSLTNHVAYTALIHGLCQDGQFSKAVKLFSDMRRYGLQP 563
           LF + ++             K  + +  AYT+L+ G  + G   +A+ L   M   G++ 
Sbjct: 635 LFEQMVQ-------------KGLVPDRTAYTSLMDGNFKQGNVLEALALRDKMAEIGMKL 694

Query: 564 DEVIYVVMLRGLFQVKYI--LMMLHADMLKFGVIPNSAVHVILCECYQESGFLKSA 615
           D + Y  ++ GL     +        +M+  G+ P+  + + + + + E G +  A
Sbjct: 695 DLLAYTSLVWGLSHCNQLQKARSFLEEMIGEGIHPDEVLCISVLKKHYELGCIDEA 737

HSP 4 Score: 209.5 bits (532), Expect = 5.8e-54
Identity = 134/489 (27.40%), Postives = 244/489 (49.90%), Query Frame = 1

Query: 30  EFFNAAPEKNIQLYSAIIHVLVGSKLLSHARYLLNDL-VQNLVKSHKPYHACQLAFSELS 89
           +   A     +  Y+ +I  +     +  AR L  ++  + LV     Y++    F ++ 
Sbjct: 252 DMIGAGARPTVFTYNIMIDCMCKEGDVEAARGLFEEMKFRGLVPDTVTYNSMIDGFGKVG 311

Query: 90  RL----------KSSKFTPNV--YGELIIVLCKMELVEEALSMYHKV---GAALTIQACN 149
           RL          K     P+V  Y  LI   CK   +   L  Y ++   G    + + +
Sbjct: 312 RLDDTVCFFEEMKDMCCEPDVITYNALINCFCKFGKLPIGLEFYREMKGNGLKPNVVSYS 371

Query: 150 VLLYVLVKTGRFELLWRIYEEMISNGLSPSVITFGTLIDGCCRQGDLLRAQEMFDEMRVK 209
            L+    K G  +   + Y +M   GL P+  T+ +LID  C+ G+L  A  + +EM   
Sbjct: 372 TLVDAFCKEGMMQQAIKFYVDMRRVGLVPNEYTYTSLIDANCKIGNLSDAFRLGNEMLQV 431

Query: 210 GIVPTVIVYTILIRGLCSDNKIEEAESMHRAMREVGVYPNVYTYNTLMDGYCKLANAKQA 269
           G+   V+ YT LI GLC   +++EAE +   M   GV PN+ +YN L+ G+ K  N  +A
Sbjct: 432 GVEWNVVTYTALIDGLCDAERMKEAEELFGKMDTAGVIPNLASYNALIHGFVKAKNMDRA 491

Query: 270 LRLYQDMLGEGLVPDVVTFGILIDGLCKFGEMKAARNLFVNMIKFSVTPNIAVYNSLIDA 329
           L L  ++ G G+ PD++ +G  I GLC   +++AA+ +   M +  +  N  +Y +L+DA
Sbjct: 492 LELLNELKGRGIKPDLLLYGTFIWGLCSLEKIEAAKVVMNEMKECGIKANSLIYTTLMDA 551

Query: 330 YCKVGDVSEAMALFLELERFEVSPDVFTYSILIRGLCSVSRTEEAGNIFEKMTKE-GILA 389
           Y K G+ +E + L  E++  ++   V T+ +LI GLC      +A + F +++ + G+ A
Sbjct: 552 YFKSGNPTEGLHLLDEMKELDIEVTVVTFCVLIDGLCKNKLVSKAVDYFNRISNDFGLQA 611

Query: 390 NSVTYNSLIDGCCKEGKMDKALEICSQMTENGVEPNVITFSTLIDGYCKIRNLQAAMGIY 449
           N+  + ++IDG CK+ +++ A  +  QM + G+ P+   +++L+DG  K  N+  A+ + 
Sbjct: 612 NAAIFTAMIDGLCKDNQVEAATTLFEQMVQKGLVPDRTAYTSLMDGNFKQGNVLEALALR 671

Query: 450 SEMVIKSLSPDVVTYTAMIDGHCKYGSMKEALKLYSDMLDNGITPNCYTISCLLDGLCKD 502
            +M    +  D++ YT+++ G      +++A     +M+  GI P+      +L    + 
Sbjct: 672 DKMAEIGMKLDLLAYTSLVWGLSHCNQLQKARSFLEEMIGEGIHPDEVLCISVLKKHYEL 731

BLAST of Cucsa.275720 vs. TAIR10
Match: AT1G63130.1 (AT1G63130.1 Tetratricopeptide repeat (TPR)-like superfamily protein)

HSP 1 Score: 288.5 bits (737), Expect = 9.7e-78
Identity = 160/494 (32.39%), Postives = 261/494 (52.83%), Query Frame = 1

Query: 82  LAFSELSRLKSSKFTPNVYGELIIVLC-----KMELVEEALSMYHKVGAALTIQACNVLL 141
           L  S   ++++   + N+Y   I++ C     ++ L    L+   K+G    I   N LL
Sbjct: 99  LVISLGEQMQNLGISHNLYTYSILINCFCRRSQLSLALAVLAKMMKLGYEPDIVTLNSLL 158

Query: 142 YVLVKTGRFELLWRIYEEMISNGLSPSVITFGTLIDGCCRQGDLLRAQEMFDEMRVKGIV 201
                  R      +  +M+  G  P   TF TLI G  R      A  + D M VKG  
Sbjct: 159 NGFCHGNRISDAVSLVGQMVEMGYQPDSFTFNTLIHGLFRHNRASEAVALVDRMVVKGCQ 218

Query: 202 PTVIVYTILIRGLCSDNKIEEAESMHRAMREVGVYPNVYTYNTLMDGYCKLANAKQALRL 261
           P ++ Y I++ GLC    I+ A S+ + M +  + P V  YNT++D  C   N   AL L
Sbjct: 219 PDLVTYGIVVNGLCKRGDIDLALSLLKKMEQGKIEPGVVIYNTIIDALCNYKNVNDALNL 278

Query: 262 YQDMLGEGLVPDVVTFGILIDGLCKFGEMKAARNLFVNMIKFSVTPNIAVYNSLIDAYCK 321
           + +M  +G+ P+VVT+  LI  LC +G    A  L  +MI+  + PN+  +++LIDA+ K
Sbjct: 279 FTEMDNKGIRPNVVTYNSLIRCLCNYGRWSDASRLLSDMIERKINPNVVTFSALIDAFVK 338

Query: 322 VGDVSEAMALFLELERFEVSPDVFTYSILIRGLCSVSRTEEAGNIFEKMTKEGILANSVT 381
            G + EA  L+ E+ +  + PD+FTYS LI G C   R +EA ++FE M  +    N VT
Sbjct: 339 EGKLVEAEKLYDEMIKRSIDPDIFTYSSLINGFCMHDRLDEAKHMFELMISKDCFPNVVT 398

Query: 382 YNSLIDGCCKEGKMDKALEICSQMTENGVEPNVITFSTLIDGYCKIRNLQAAMGIYSEMV 441
           YN+LI G CK  ++D+ +E+  +M++ G+  N +T++TLI G+ + R    A  ++ +MV
Sbjct: 399 YNTLIKGFCKAKRVDEGMELFREMSQRGLVGNTVTYTTLIHGFFQARECDNAQIVFKQMV 458

Query: 442 IKSLSPDVVTYTAMIDGHCKYGSMKEALKLYSDMLDNGITPNCYTISCLLDGLCKDGKIS 501
              + PD++TY+ ++DG C  G ++ AL ++  +  + + P+ YT + +++G+CK GK+ 
Sbjct: 459 SDGVLPDIMTYSILLDGLCNNGKVETALVVFEYLQRSKMEPDIYTYNIMIEGMCKAGKVE 518

Query: 502 DALELFTEKIEFQTPRCNVDAGGSKPSLTNHVAYTALIHGLCQDGQFSKAVKLFSDMRRY 561
           D  +LF          C++   G KP   N V YT ++ G C+ G   +A  LF +M+  
Sbjct: 519 DGWDLF----------CSLSLKGVKP---NVVTYTTMMSGFCRKGLKEEADALFREMKEE 578

Query: 562 GLQPDEVIYVVMLR 571
           G  PD   Y  ++R
Sbjct: 579 GPLPDSGTYNTLIR 579

HSP 2 Score: 215.3 bits (547), Expect = 1.0e-55
Identity = 123/434 (28.34%), Postives = 222/434 (51.15%), Query Frame = 1

Query: 183 AQEMFDEMRVKGIVPTVIVYTILIRGLCSDNKIEEAESMHRAMREVGVYPNVYTYNTLMD 242
           A  +F +M      P+++ ++ L+  +   NK +   S+   M+ +G+  N+YTY+ L++
Sbjct: 65  AVNLFGDMVKSRPFPSIVEFSKLLSAIAKMNKFDLVISLGEQMQNLGISHNLYTYSILIN 124

Query: 243 GYCKLANAKQALRLYQDMLGEGLVPDVVTFGILIDGLCKFGEMKAARNLFVNMIKFSVTP 302
            +C+ +    AL +   M+  G  PD+VT   L++G C    +  A +L   M++    P
Sbjct: 125 CFCRRSQLSLALAVLAKMMKLGYEPDIVTLNSLLNGFCHGNRISDAVSLVGQMVEMGYQP 184

Query: 303 NIAVYNSLIDAYCKVGDVSEAMALFLELERFEVSPDVFTYSILIRGLCSVSRTEEAGNIF 362
           +   +N+LI    +    SEA+AL   +      PD+ TY I++ GLC     + A ++ 
Sbjct: 185 DSFTFNTLIHGLFRHNRASEAVALVDRMVVKGCQPDLVTYGIVVNGLCKRGDIDLALSLL 244

Query: 363 EKMTKEGILANSVTYNSLIDGCCKEGKMDKALEICSQMTENGVEPNVITFSTLIDGYCKI 422
           +KM +  I    V YN++ID  C    ++ AL + ++M   G+ PNV+T+++LI   C  
Sbjct: 245 KKMEQGKIEPGVVIYNTIIDALCNYKNVNDALNLFTEMDNKGIRPNVVTYNSLIRCLCNY 304

Query: 423 RNLQAAMGIYSEMVIKSLSPDVVTYTAMIDGHCKYGSMKEALKLYSDMLDNGITPNCYTI 482
                A  + S+M+ + ++P+VVT++A+ID   K G + EA KLY +M+   I P+ +T 
Sbjct: 305 GRWSDASRLLSDMIERKINPNVVTFSALIDAFVKEGKLVEAEKLYDEMIKRSIDPDIFTY 364

Query: 483 SCLLDGLCKDGKISDALELFTEKIEFQTPRCNVDAGGSKPSLTNHVAYTALIHGLCQDGQ 542
           S L++G C   ++ +A  +F   I             SK    N V Y  LI G C+  +
Sbjct: 365 SSLINGFCMHDRLDEAKHMFELMI-------------SKDCFPNVVTYNTLIKGFCKAKR 424

Query: 543 FSKAVKLFSDMRRYGLQPDEVIYVVMLRGLFQVKYI--LMMLHADMLKFGVIPNSAVHVI 602
             + ++LF +M + GL  + V Y  ++ G FQ +      ++   M+  GV+P+   + I
Sbjct: 425 VDEGMELFREMSQRGLVGNTVTYTTLIHGFFQARECDNAQIVFKQMVSDGVLPDIMTYSI 484

Query: 603 LCECYQESGFLKSA 615
           L +    +G +++A
Sbjct: 485 LLDGLCNNGKVETA 485

HSP 3 Score: 191.8 bits (486), Expect = 1.2e-48
Identity = 112/416 (26.92%), Postives = 204/416 (49.04%), Query Frame = 1

Query: 82  LAFSELSRLKSSKFTPNV--YGELIIVLCKMELVEEALSMYHKVGAALTIQACNVLLYVL 141
           LA S L +++  K  P V  Y  +I  LC  + V +AL+++                   
Sbjct: 239 LALSLLKKMEQGKIEPGVVIYNTIIDALCNYKNVNDALNLF------------------- 298

Query: 142 VKTGRFELLWRIYEEMISNGLSPSVITFGTLIDGCCRQGDLLRAQEMFDEMRVKGIVPTV 201
                         EM + G+ P+V+T+ +LI   C  G    A  +  +M  + I P V
Sbjct: 299 -------------TEMDNKGIRPNVVTYNSLIRCLCNYGRWSDASRLLSDMIERKINPNV 358

Query: 202 IVYTILIRGLCSDNKIEEAESMHRAMREVGVYPNVYTYNTLMDGYCKLANAKQALRLYQD 261
           + ++ LI     + K+ EAE ++  M +  + P+++TY++L++G+C      +A  +++ 
Sbjct: 359 VTFSALIDAFVKEGKLVEAEKLYDEMIKRSIDPDIFTYSSLINGFCMHDRLDEAKHMFEL 418

Query: 262 MLGEGLVPDVVTFGILIDGLCKFGEMKAARNLFVNMIKFSVTPNIAVYNSLIDAYCKVGD 321
           M+ +   P+VVT+  LI G CK   +     LF  M +  +  N   Y +LI  + +  +
Sbjct: 419 MISKDCFPNVVTYNTLIKGFCKAKRVDEGMELFREMSQRGLVGNTVTYTTLIHGFFQARE 478

Query: 322 VSEAMALFLELERFEVSPDVFTYSILIRGLCSVSRTEEAGNIFEKMTKEGILANSVTYNS 381
              A  +F ++    V PD+ TYSIL+ GLC+  + E A  +FE + +  +  +  TYN 
Sbjct: 479 CDNAQIVFKQMVSDGVLPDIMTYSILLDGLCNNGKVETALVVFEYLQRSKMEPDIYTYNI 538

Query: 382 LIDGCCKEGKMDKALEICSQMTENGVEPNVITFSTLIDGYCKIRNLQAAMGIYSEMVIKS 441
           +I+G CK GK++   ++   ++  GV+PNV+T++T++ G+C+    + A  ++ EM  + 
Sbjct: 539 MIEGMCKAGKVEDGWDLFCSLSLKGVKPNVVTYTTMMSGFCRKGLKEEADALFREMKEEG 598

Query: 442 LSPDVVTYTAMIDGHCKYGSMKEALKLYSDMLDNGITPNCYTISCLLDGLCKDGKI 496
             PD  TY  +I  H + G    + +L  +M       +  TI  L+  +  DG++
Sbjct: 599 PLPDSGTYNTLIRAHLRDGDKAASAELIREMRSCRFVGDASTIG-LVTNMLHDGRL 621

HSP 4 Score: 116.7 bits (291), Expect = 5.1e-26
Identity = 91/356 (25.56%), Postives = 163/356 (45.79%), Query Frame = 1

Query: 13  DSLITTVLNCRSPWKALEFF----NAAPEKNIQLYSAIIHVLVG-------SKLLSHAR- 72
           +++I  + N ++   AL  F    N     N+  Y+++I  L         S+LLS    
Sbjct: 260 NTIIDALCNYKNVNDALNLFTEMDNKGIRPNVVTYNSLIRCLCNYGRWSDASRLLSDMIE 319

Query: 73  -------YLLNDLVQNLVKSHKPYHACQLAFSELSR-LKSSKFTPNVYGELIIVLCKMEL 132
                     + L+   VK  K   A +L    + R +    FT   Y  LI   C  + 
Sbjct: 320 RKINPNVVTFSALIDAFVKEGKLVEAEKLYDEMIKRSIDPDIFT---YSSLINGFCMHDR 379

Query: 133 VEEALSMYHKV---GAALTIQACNVLLYVLVKTGRFELLWRIYEEMISNGLSPSVITFGT 192
           ++EA  M+  +        +   N L+    K  R +    ++ EM   GL  + +T+ T
Sbjct: 380 LDEAKHMFELMISKDCFPNVVTYNTLIKGFCKAKRVDEGMELFREMSQRGLVGNTVTYTT 439

Query: 193 LIDGCCRQGDLLRAQEMFDEMRVKGIVPTVIVYTILIRGLCSDNKIEEAESMHRAMREVG 252
           LI G  +  +   AQ +F +M   G++P ++ Y+IL+ GLC++ K+E A  +   ++   
Sbjct: 440 LIHGFFQARECDNAQIVFKQMVSDGVLPDIMTYSILLDGLCNNGKVETALVVFEYLQRSK 499

Query: 253 VYPNVYTYNTLMDGYCKLANAKQALRLYQDMLGEGLVPDVVTFGILIDGLCKFGEMKAAR 312
           + P++YTYN +++G CK    +    L+  +  +G+ P+VVT+  ++ G C+ G  + A 
Sbjct: 500 MEPDIYTYNIMIEGMCKAGKVEDGWDLFCSLSLKGVKPNVVTYTTMMSGFCRKGLKEEAD 559

Query: 313 NLFVNMIKFSVTPNIAVYNSLIDAYCKVGDVSEAMALFLELERFEVSPDVFTYSIL 346
            LF  M +    P+   YN+LI A+ + GD + +  L  E+       D  T  ++
Sbjct: 560 ALFREMKEEGPLPDSGTYNTLIRAHLRDGDKAASAELIREMRSCRFVGDASTIGLV 612

BLAST of Cucsa.275720 vs. TAIR10
Match: AT1G12700.1 (AT1G12700.1 ATP binding;nucleic acid binding;helicases)

HSP 1 Score: 277.3 bits (708), Expect = 2.2e-74
Identity = 147/476 (30.88%), Postives = 249/476 (52.31%), Query Frame = 1

Query: 86  ELSRLKSSKFTPNVYGELIIVLCKMELVEEALSMYHKVGAALTIQACNVLLYVLVKTGRF 145
           EL+ +  + +T N+        CK       L    K+G        N L+  L   G+ 
Sbjct: 115 ELNGIAHNIYTLNIMINCFCRCCKTCFAYSVLGKVMKLGYEPDTTTFNTLIKGLFLEGKV 174

Query: 146 ELLWRIYEEMISNGLSPSVITFGTLIDGCCRQGDLLRAQEMFDEMRVKGIVPTVIVYTIL 205
                + + M+ NG  P V+T+ ++++G CR GD   A ++  +M  + +   V  Y+ +
Sbjct: 175 SEAVVLVDRMVENGCQPDVVTYNSIVNGICRSGDTSLALDLLRKMEERNVKADVFTYSTI 234

Query: 206 IRGLCSDNKIEEAESMHRAMREVGVYPNVYTYNTLMDGYCKLANAKQALRLYQDMLGEGL 265
           I  LC D  I+ A S+ + M   G+  +V TYN+L+ G CK         L +DM+   +
Sbjct: 235 IDSLCRDGCIDAAISLFKEMETKGIKSSVVTYNSLVRGLCKAGKWNDGALLLKDMVSREI 294

Query: 266 VPDVVTFGILIDGLCKFGEMKAARNLFVNMIKFSVTPNIAVYNSLIDAYCKVGDVSEAMA 325
           VP+V+TF +L+D   K G+++ A  L+  MI   ++PNI  YN+L+D YC    +SEA  
Sbjct: 295 VPNVITFNVLLDVFVKEGKLQEANELYKEMITRGISPNIITYNTLMDGYCMQNRLSEANN 354

Query: 326 LFLELERFEVSPDVFTYSILIRGLCSVSRTEEAGNIFEKMTKEGILANSVTYNSLIDGCC 385
           +   + R + SPD+ T++ LI+G C V R ++   +F  ++K G++AN+VTY+ L+ G C
Sbjct: 355 MLDLMVRNKCSPDIVTFTSLIKGYCMVKRVDDGMKVFRNISKRGLVANAVTYSILVQGFC 414

Query: 386 KEGKMDKALEICSQMTENGVEPNVITFSTLIDGYCKIRNLQAAMGIYSEMVIKSLSPDVV 445
           + GK+  A E+  +M  +GV P+V+T+  L+DG C    L+ A+ I+ ++    +   +V
Sbjct: 415 QSGKIKLAEELFQEMVSHGVLPDVMTYGILLDGLCDNGKLEKALEIFEDLQKSKMDLGIV 474

Query: 446 TYTAMIDGHCKYGSMKEALKLYSDMLDNGITPNCYTISCLLDGLCKDGKISDALELFTEK 505
            YT +I+G CK G +++A  L+  +   G+ PN  T + ++ GLCK G +S+A  L  + 
Sbjct: 475 MYTTIIEGMCKGGKVEDAWNLFCSLPCKGVKPNVMTYTVMISGLCKKGSLSEANILLRK- 534

Query: 506 IEFQTPRCNVDAGGSKPSLTNHVAYTALIHGLCQDGQFSKAVKLFSDMRRYGLQPD 562
                    ++  G+ P   N   Y  LI    +DG  + + KL  +M+  G   D
Sbjct: 535 ---------MEEDGNAP---NDCTYNTLIRAHLRDGDLTASAKLIEEMKSCGFSAD 577

HSP 2 Score: 247.3 bits (630), Expect = 2.5e-65
Identity = 137/478 (28.66%), Postives = 249/478 (52.09%), Query Frame = 1

Query: 139 LVKTGRFELLWRIYEEMISNGLSPSVITFGTLIDGCCRQGDLLRAQEMFDEMRVKGIVPT 198
           + +T +F L+    +++  NG++ ++ T   +I+  CR      A  +  ++   G  P 
Sbjct: 98  IARTKQFNLVLDFCKQLELNGIAHNIYTLNIMINCFCRCCKTCFAYSVLGKVMKLGYEPD 157

Query: 199 VIVYTILIRGLCSDNKIEEAESMHRAMREVGVYPNVYTYNTLMDGYCKLANAKQALRLYQ 258
              +  LI+GL  + K+ EA  +   M E G  P+V TYN++++G C+  +   AL L +
Sbjct: 158 TTTFNTLIKGLFLEGKVSEAVVLVDRMVENGCQPDVVTYNSIVNGICRSGDTSLALDLLR 217

Query: 259 DMLGEGLVPDVVTFGILIDGLCKFGEMKAARNLFVNMIKFSVTPNIAVYNSLIDAYCKVG 318
            M    +  DV T+  +ID LC+ G + AA +LF  M    +  ++  YNSL+   CK G
Sbjct: 218 KMEERNVKADVFTYSTIIDSLCRDGCIDAAISLFKEMETKGIKSSVVTYNSLVRGLCKAG 277

Query: 319 DVSEAMALFLELERFEVSPDVFTYSILIRGLCSVSRTEEAGNIFEKMTKEGILANSVTYN 378
             ++   L  ++   E+ P+V T+++L+       + +EA  ++++M   GI  N +TYN
Sbjct: 278 KWNDGALLLKDMVSREIVPNVITFNVLLDVFVKEGKLQEANELYKEMITRGISPNIITYN 337

Query: 379 SLIDGCCKEGKMDKALEICSQMTENGVEPNVITFSTLIDGYCKIRNLQAAMGIYSEMVIK 438
           +L+DG C + ++ +A  +   M  N   P+++TF++LI GYC ++ +   M ++  +  +
Sbjct: 338 TLMDGYCMQNRLSEANNMLDLMVRNKCSPDIVTFTSLIKGYCMVKRVDDGMKVFRNISKR 397

Query: 439 SLSPDVVTYTAMIDGHCKYGSMKEALKLYSDMLDNGITPNCYTISCLLDGLCKDGKISDA 498
            L  + VTY+ ++ G C+ G +K A +L+ +M+ +G+ P+  T   LLDGLC +GK+  A
Sbjct: 398 GLVANAVTYSILVQGFCQSGKIKLAEELFQEMVSHGVLPDVMTYGILLDGLCDNGKLEKA 457

Query: 499 LELFTEKIEFQTPRCNVDAGGSKPSLTNHVAYTALIHGLCQDGQFSKAVKLFSDMRRYGL 558
           LE+F +       +  +D G         V YT +I G+C+ G+   A  LF  +   G+
Sbjct: 458 LEIFED-----LQKSKMDLG--------IVMYTTIIEGMCKGGKVEDAWNLFCSLPCKGV 517

Query: 559 QPDEVIYVVMLRGLFQVKYI--LMMLHADMLKFGVIPNSAVHVILCECYQESGFLKSA 615
           +P+ + Y VM+ GL +   +    +L   M + G  PN   +  L   +   G L ++
Sbjct: 518 KPNVMTYTVMISGLCKKGSLSEANILLRKMEEDGNAPNDCTYNTLIRAHLRDGDLTAS 562

HSP 3 Score: 106.7 bits (265), Expect = 5.2e-23
Identity = 77/329 (23.40%), Postives = 149/329 (45.29%), Query Frame = 1

Query: 295 MIKFSVTPNIAVYNSLIDAYCKVGDVSEAM-----ALFLELER--FEVSPDVFTYSILIR 354
           MIK S+T N+     +     K G +   +     + F   ER    +S     +   +R
Sbjct: 2   MIKRSITTNMKALRLIQPHLLKTGSLRTDLLCTISSFFSSCERDFSSISNGNVCFRERLR 61

Query: 355 GLCSVSRTEEAGNIFEKMTKEGILANSVTYNSLIDGCCKEGKMDKALEICSQMTENGVEP 414
                 + ++A  +F++M +   L + V ++       +  + +  L+ C Q+  NG+  
Sbjct: 62  SGIVDIKKDDAIALFQEMIRSRPLPSLVDFSRFFSAIARTKQFNLVLDFCKQLELNGIAH 121

Query: 415 NVITFSTLIDGYCKIRNLQAAMGIYSEMVIKSLSPDVVTYTAMIDGHCKYGSMKEALKLY 474
           N+ T + +I+ +C+      A  +  +++     PD  T+  +I G    G + EA+ L 
Sbjct: 122 NIYTLNIMINCFCRCCKTCFAYSVLGKVMKLGYEPDTTTFNTLIKGLFLEGKVSEAVVLV 181

Query: 475 SDMLDNGITPNCYTISCLLDGLCKDGKISDALELFTEKIEFQTPRCNVDAGGSKPSLTNH 534
             M++NG  P+  T + +++G+C+ G  S AL+L   K+E +  + +V            
Sbjct: 182 DRMVENGCQPDVVTYNSIVNGICRSGDTSLALDLL-RKMEERNVKADV------------ 241

Query: 535 VAYTALIHGLCQDGQFSKAVKLFSDMRRYGLQPDEVIYVVMLRGLFQVKYIL--MMLHAD 594
             Y+ +I  LC+DG    A+ LF +M   G++   V Y  ++RGL +        +L  D
Sbjct: 242 FTYSTIIDSLCRDGCIDAAISLFKEMETKGIKSSVVTYNSLVRGLCKAGKWNDGALLLKD 301

Query: 595 MLKFGVIPNSAVHVILCECYQESGFLKSA 615
           M+   ++PN     +L + + + G L+ A
Sbjct: 302 MVSREIVPNVITFNVLLDVFVKEGKLQEA 317

BLAST of Cucsa.275720 vs. TAIR10
Match: AT5G59900.1 (AT5G59900.1 Pentatricopeptide repeat (PPR) superfamily protein)

HSP 1 Score: 277.3 bits (708), Expect = 2.2e-74
Identity = 153/423 (36.17%), Postives = 234/423 (55.32%), Query Frame = 1

Query: 151 IYEEMISN-GLSPSVITFGTLIDGCCRQGDLLRAQEMFDEMRVKGIVPTVIVYTILIRGL 210
           +++ MI+   L P V T   L+ G  +      A E+F++M   GI P V +YT +IR L
Sbjct: 178 VFKMMITKVSLLPEVRTLSALLHGLVKFRHFGLAMELFNDMVSVGIRPDVYIYTGVIRSL 237

Query: 211 CSDNKIEEAESMHRAMREVGVYPNVYTYNTLMDGYCKLANAKQALRLYQDMLGEGLVPDV 270
           C    +  A+ M   M   G   N+  YN L+DG CK     +A+ + +D+ G+ L PDV
Sbjct: 238 CELKDLSRAKEMIAHMEATGCDVNIVPYNVLIDGLCKKQKVWEAVGIKKDLAGKDLKPDV 297

Query: 271 VTFGILIDGLCKFGEMKAARNLFVNMIKFSVTPNIAVYNSLIDAYCKVGDVSEAMALFLE 330
           VT+  L+ GLCK  E +    +   M+    +P+ A  +SL++   K G + EA+ L   
Sbjct: 298 VTYCTLVYGLCKVQEFEIGLEMMDEMLCLRFSPSEAAVSSLVEGLRKRGKIEEALNLVKR 357

Query: 331 LERFEVSPDVFTYSILIRGLCSVSRTEEAGNIFEKMTKEGILANSVTYNSLIDGCCKEGK 390
           +  F VSP++F Y+ LI  LC   +  EA  +F++M K G+  N VTY+ LID  C+ GK
Sbjct: 358 VVDFGVSPNLFVYNALIDSLCKGRKFHEAELLFDRMGKIGLRPNDVTYSILIDMFCRRGK 417

Query: 391 MDKALEICSQMTENGVEPNVITFSTLIDGYCKIRNLQAAMGIYSEMVIKSLSPDVVTYTA 450
           +D AL    +M + G++ +V  +++LI+G+CK  ++ AA G  +EM+ K L P VVTYT+
Sbjct: 418 LDTALSFLGEMVDTGLKLSVYPYNSLINGHCKFGDISAAEGFMAEMINKKLEPTVVTYTS 477

Query: 451 MIDGHCKYGSMKEALKLYSDMLDNGITPNCYTISCLLDGLCKDGKISDALELFTEKIEFQ 510
           ++ G+C  G + +AL+LY +M   GI P+ YT + LL GL + G I DA++LF E  E+ 
Sbjct: 478 LMGGYCSKGKINKALRLYHEMTGKGIAPSIYTFTTLLSGLFRAGLIRDAVKLFNEMAEWN 537

Query: 511 TPRCNVDAGGSKPSLTNHVAYTALIHGLCQDGQFSKAVKLFSDMRRYGLQPDEVIYVVML 570
                      KP   N V Y  +I G C++G  SKA +   +M   G+ PD   Y  ++
Sbjct: 538 V----------KP---NRVTYNVMIEGYCEEGDMSKAFEFLKEMTEKGIVPDTYSYRPLI 587

Query: 571 RGL 573
            GL
Sbjct: 598 HGL 587

HSP 2 Score: 258.5 bits (659), Expect = 1.1e-68
Identity = 166/554 (29.96%), Postives = 273/554 (49.28%), Query Frame = 1

Query: 25  PWKALEFFNAAP-----EKNIQLYSAIIHVLVGSKLLSHARYLLNDLVQNLVKSHKPYHA 84
           P   L FFN        + +   +  +IH LV + L   A  LL  L   L+++ KP   
Sbjct: 84  PKLGLRFFNFLGLHRGFDHSTASFCILIHALVKANLFWPASSLLQTL---LLRALKPSDV 143

Query: 85  CQLAFS--ELSRLKSSKFTPNVYGELIIVLCKMELVEEALSMYHKVGAALTIQACNVLLY 144
             + FS  E  +L SS     +    +     ++ V     M  KV     ++  + LL+
Sbjct: 144 FNVLFSCYEKCKLSSSSSFDLLIQHYVRSRRVLDGVLVFKMMITKVSLLPEVRTLSALLH 203

Query: 145 VLVKTGRFELLWRIYEEMISNGLSPSVITFGTLIDGCCRQGDLLRAQEMFDEMRVKGIVP 204
            LVK   F L   ++ +M+S G+ P V  +  +I   C   DL RA+EM   M   G   
Sbjct: 204 GLVKFRHFGLAMELFNDMVSVGIRPDVYIYTGVIRSLCELKDLSRAKEMIAHMEATGCDV 263

Query: 205 TVIVYTILIRGLCSDNKIEEAESMHRAMREVGVYPNVYTYNTLMDGYCKLANAKQALRLY 264
            ++ Y +LI GLC   K+ EA  + + +    + P+V TY TL+ G CK+   +  L + 
Sbjct: 264 NIVPYNVLIDGLCKKQKVWEAVGIKKDLAGKDLKPDVVTYCTLVYGLCKVQEFEIGLEMM 323

Query: 265 QDMLGEGLVPDVVTFGILIDGLCKFGEMKAARNLFVNMIKFSVTPNIAVYNSLIDAYCKV 324
            +ML     P       L++GL K G+++ A NL   ++ F V+PN+ VYN+LID+ CK 
Sbjct: 324 DEMLCLRFSPSEAAVSSLVEGLRKRGKIEEALNLVKRVVDFGVSPNLFVYNALIDSLCKG 383

Query: 325 GDVSEAMALFLELERFEVSPDVFTYSILIRGLCSVSRTEEAGNIFEKMTKEGILANSVTY 384
               EA  LF  + +  + P+  TYSILI   C   + + A +   +M   G+  +   Y
Sbjct: 384 RKFHEAELLFDRMGKIGLRPNDVTYSILIDMFCRRGKLDTALSFLGEMVDTGLKLSVYPY 443

Query: 385 NSLIDGCCKEGKMDKALEICSQMTENGVEPNVITFSTLIDGYCKIRNLQAAMGIYSEMVI 444
           NSLI+G CK G +  A    ++M    +EP V+T+++L+ GYC    +  A+ +Y EM  
Sbjct: 444 NSLINGHCKFGDISAAEGFMAEMINKKLEPTVVTYTSLMGGYCSKGKINKALRLYHEMTG 503

Query: 445 KSLSPDVVTYTAMIDGHCKYGSMKEALKLYSDMLDNGITPNCYTISCLLDGLCKDGKISD 504
           K ++P + T+T ++ G  + G +++A+KL+++M +  + PN  T + +++G C++G +S 
Sbjct: 504 KGIAPSIYTFTTLLSGLFRAGLIRDAVKLFNEMAEWNVKPNRVTYNVMIEGYCEEGDMSK 563

Query: 505 ALELFTEKIEFQTPRCNVDAGGSKPSLTNHVAYTALIHGLCQDGQFSKAVKLFSDMRRYG 564
           A E   E  E             K  + +  +Y  LIHGLC  GQ S+A      + +  
Sbjct: 564 AFEFLKEMTE-------------KGIVPDTYSYRPLIHGLCLTGQASEAKVFVDGLHKGN 621

Query: 565 LQPDEVIYVVMLRG 572
            + +E+ Y  +L G
Sbjct: 624 CELNEICYTGLLHG 621

HSP 3 Score: 257.7 bits (657), Expect = 1.8e-68
Identity = 157/497 (31.59%), Postives = 254/497 (51.11%), Query Frame = 1

Query: 81  QLAFSELSRLKSSKFTPN--VYGELIIVLCKMELVEEALSMYHKV---GAALTIQACNVL 140
           ++    +  +   +F+P+      L+  L K   +EEAL++  +V   G +  +   N L
Sbjct: 314 EIGLEMMDEMLCLRFSPSEAAVSSLVEGLRKRGKIEEALNLVKRVVDFGVSPNLFVYNAL 373

Query: 141 LYVLVKTGRFELLWRIYEEMISNGLSPSVITFGTLIDGCCRQGDLLRAQEMFDEMRVKGI 200
           +  L K  +F     +++ M   GL P+ +T+  LID  CR+G L  A     EM   G+
Sbjct: 374 IDSLCKGRKFHEAELLFDRMGKIGLRPNDVTYSILIDMFCRRGKLDTALSFLGEMVDTGL 433

Query: 201 VPTVIVYTILIRGLCSDNKIEEAESMHRAMREVGVYPNVYTYNTLMDGYCKLANAKQALR 260
             +V  Y  LI G C    I  AE     M    + P V TY +LM GYC      +ALR
Sbjct: 434 KLSVYPYNSLINGHCKFGDISAAEGFMAEMINKKLEPTVVTYTSLMGGYCSKGKINKALR 493

Query: 261 LYQDMLGEGLVPDVVTFGILIDGLCKFGEMKAARNLFVNMIKFSVTPNIAVYNSLIDAYC 320
           LY +M G+G+ P + TF  L+ GL + G ++ A  LF  M +++V PN   YN +I+ YC
Sbjct: 494 LYHEMTGKGIAPSIYTFTTLLSGLFRAGLIRDAVKLFNEMAEWNVKPNRVTYNVMIEGYC 553

Query: 321 KVGDVSEAMALFLELERFEVSPDVFTYSILIRGLCSVSRTEEAGNIFEKMTKEGILANSV 380
           + GD+S+A     E+    + PD ++Y  LI GLC   +  EA    + + K     N +
Sbjct: 554 EEGDMSKAFEFLKEMTEKGIVPDTYSYRPLIHGLCLTGQASEAKVFVDGLHKGNCELNEI 613

Query: 381 TYNSLIDGCCKEGKMDKALEICSQMTENGVEPNVITFSTLIDGYCKIRNLQAAMGIYSEM 440
            Y  L+ G C+EGK+++AL +C +M + GV+ +++ +  LIDG  K ++ +   G+  EM
Sbjct: 614 CYTGLLHGFCREGKLEEALSVCQEMVQRGVDLDLVCYGVLIDGSLKHKDRKLFFGLLKEM 673

Query: 441 VIKSLSPDVVTYTAMIDGHCKYGSMKEALKLYSDMLDNGITPNCYTISCLLDGLCKDGKI 500
             + L PD V YT+MID   K G  KEA  ++  M++ G  PN  T + +++GLCK G +
Sbjct: 674 HDRGLKPDDVIYTSMIDAKSKTGDFKEAFGIWDLMINEGCVPNEVTYTAVINGLCKAGFV 733

Query: 501 SDALELFTEKIEFQTPRCNVDAGGSKPSLTNHVAYTALIHGLCQ-DGQFSKAVKLFSDMR 560
           ++A E+   K++               S+ N V Y   +  L + +    KAV+L + + 
Sbjct: 734 NEA-EVLCSKMQ------------PVSSVPNQVTYGCFLDILTKGEVDMQKAVELHNAIL 793

Query: 561 RYGLQPDEVIYVVMLRG 572
           + GL  +   Y +++RG
Sbjct: 794 K-GLLANTATYNMLIRG 796

HSP 4 Score: 241.5 bits (615), Expect = 1.4e-63
Identity = 180/635 (28.35%), Postives = 304/635 (47.87%), Query Frame = 1

Query: 1   MLQVVSLSSLTPDSLITTVLNCRSPWK--ALEFFNAAPEKNIQLYSAIIHVLVGSKLLSH 60
           +LQ + L +L P  +   + +C    K  +   F+   +  ++    +  VLV   +++ 
Sbjct: 126 LLQTLLLRALKPSDVFNVLFSCYEKCKLSSSSSFDLLIQHYVRSRRVLDGVLVFKMMITK 185

Query: 61  ARYL-----LNDLVQNLVKSHKPYHACQLAFSELSRLKSSKFTPNVY--GELIIVLCKME 120
              L     L+ L+  LVK    +    LA    + + S    P+VY    +I  LC+++
Sbjct: 186 VSLLPEVRTLSALLHGLVK----FRHFGLAMELFNDMVSVGIRPDVYIYTGVIRSLCELK 245

Query: 121 LVEEALSMY---HKVGAALTIQACNVLLYVLVKTGRFELLWRIYEEMISNGLSPSVITFG 180
            +  A  M       G  + I   NVL+  L K  +      I +++    L P V+T+ 
Sbjct: 246 DLSRAKEMIAHMEATGCDVNIVPYNVLIDGLCKKQKVWEAVGIKKDLAGKDLKPDVVTYC 305

Query: 181 TLIDGCCRQGDLLRAQEMFDEMRVKGIVPTVIVYTILIRGLCSDNKIEEAESMHRAMREV 240
           TL+ G C+  +     EM DEM      P+    + L+ GL    KIEEA ++ + + + 
Sbjct: 306 TLVYGLCKVQEFEIGLEMMDEMLCLRFSPSEAAVSSLVEGLRKRGKIEEALNLVKRVVDF 365

Query: 241 GVYPNVYTYNTLMDGYCKLANAKQALRLYQDMLGEGLVPDVVTFGILIDGLCKFGEMKAA 300
           GV PN++ YN L+D  CK     +A  L+  M   GL P+ VT+ ILID  C+ G++  A
Sbjct: 366 GVSPNLFVYNALIDSLCKGRKFHEAELLFDRMGKIGLRPNDVTYSILIDMFCRRGKLDTA 425

Query: 301 RNLFVNMIKFSVTPNIAVYNSLIDAYCKVGDVSEAMALFLELERFEVSPDVFTYSILIRG 360
            +    M+   +  ++  YNSLI+ +CK GD+S A     E+   ++ P V TY+ L+ G
Sbjct: 426 LSFLGEMVDTGLKLSVYPYNSLINGHCKFGDISAAEGFMAEMINKKLEPTVVTYTSLMGG 485

Query: 361 LCSVSRTEEAGNIFEKMTKEGILANSVTYNSLIDGCCKEGKMDKALEICSQMTENGVEPN 420
            CS  +  +A  ++ +MT +GI  +  T+ +L+ G  + G +  A+++ ++M E  V+PN
Sbjct: 486 YCSKGKINKALRLYHEMTGKGIAPSIYTFTTLLSGLFRAGLIRDAVKLFNEMAEWNVKPN 545

Query: 421 VITFSTLIDGYCKIRNLQAAMGIYSEMVIKSLSPDVVTYTAMIDGHCKYGSMKEALKLYS 480
            +T++ +I+GYC+  ++  A     EM  K + PD  +Y  +I G C  G   EA K++ 
Sbjct: 546 RVTYNVMIEGYCEEGDMSKAFEFLKEMTEKGIVPDTYSYRPLIHGLCLTGQASEA-KVFV 605

Query: 481 DMLDNG---ITPNCYTISCLLDGLCKDGKISDALELFTEKIEFQTPRCNVDAGGSKPSLT 540
           D L  G   +   CYT   LL G C++GK+ +AL +  E ++       VD         
Sbjct: 606 DGLHKGNCELNEICYT--GLLHGFCREGKLEEALSVCQEMVQ-----RGVD--------L 665

Query: 541 NHVAYTALIHGLCQDGQFSKAVKLFSDMRRYGLQPDEVIYVVML-----RGLFQVKYILM 600
           + V Y  LI G  +         L  +M   GL+PD+VIY  M+      G F+  + + 
Sbjct: 666 DLVCYGVLIDGSLKHKDRKLFFGLLKEMHDRGLKPDDVIYTSMIDAKSKTGDFKEAFGIW 725

Query: 601 MLHADMLKFGVIPNSAVHVILCECYQESGFLKSAQ 616
            L   M+  G +PN   +  +     ++GF+  A+
Sbjct: 726 DL---MINEGCVPNEVTYTAVINGLCKAGFVNEAE 737

HSP 5 Score: 235.7 bits (600), Expect = 7.5e-62
Identity = 157/544 (28.86%), Postives = 269/544 (49.45%), Query Frame = 1

Query: 61  YLLNDLVQNLVKSHKPYHACQLAFSELSRLKSSKFTPN--VYGELIIVLCKMELVEEALS 120
           ++ N L+ +L K  K +H  +L F  + ++      PN   Y  LI + C+   ++ ALS
Sbjct: 368 FVYNALIDSLCKGRK-FHEAELLFDRMGKIG---LRPNDVTYSILIDMFCRRGKLDTALS 427

Query: 121 MYHKV---GAALTIQACNVLLYVLVKTGRFELLWRIYEEMISNGLSPSVITFGTLIDGCC 180
              ++   G  L++   N L+    K G          EMI+  L P+V+T+ +L+ G C
Sbjct: 428 FLGEMVDTGLKLSVYPYNSLINGHCKFGDISAAEGFMAEMINKKLEPTVVTYTSLMGGYC 487

Query: 181 RQGDLLRAQEMFDEMRVKGIVPTVIVYTILIRGLCSDNKIEEAESMHRAMREVGVYPNVY 240
            +G + +A  ++ EM  KGI P++  +T L+ GL     I +A  +   M E  V PN  
Sbjct: 488 SKGKINKALRLYHEMTGKGIAPSIYTFTTLLSGLFRAGLIRDAVKLFNEMAEWNVKPNRV 547

Query: 241 TYNTLMDGYCKLANAKQALRLYQDMLGEGLVPDVVTFGILIDGLCKFGEMKAARNLFVNM 300
           TYN +++GYC+  +  +A    ++M  +G+VPD  ++  LI GLC  G+   A+     +
Sbjct: 548 TYNVMIEGYCEEGDMSKAFEFLKEMTEKGIVPDTYSYRPLIHGLCLTGQASEAKVFVDGL 607

Query: 301 IKFSVTPNIAVYNSLIDAYCKVGDVSEAMALFLELERFEVSPDVFTYSILIRGLCSVSRT 360
            K +   N   Y  L+  +C+ G + EA+++  E+ +  V  D+  Y +LI G       
Sbjct: 608 HKGNCELNEICYTGLLHGFCREGKLEEALSVCQEMVQRGVDLDLVCYGVLIDGSLKHKDR 667

Query: 361 EEAGNIFEKMTKEGILANSVTYNSLIDGCCKEGKMDKALEICSQMTENGVEPNVITFSTL 420
           +    + ++M   G+  + V Y S+ID   K G   +A  I   M   G  PN +T++ +
Sbjct: 668 KLFFGLLKEMHDRGLKPDDVIYTSMIDAKSKTGDFKEAFGIWDLMINEGCVPNEVTYTAV 727

Query: 421 IDGYCKIRNLQAAMGIYSEMVIKSLSPDVVTYTAMIDGHCKYG-SMKEALKLYSDMLDNG 480
           I+G CK   +  A  + S+M   S  P+ VTY   +D   K    M++A++L++ +L  G
Sbjct: 728 INGLCKAGFVNEAEVLCSKMQPVSSVPNQVTYGCFLDILTKGEVDMQKAVELHNAIL-KG 787

Query: 481 ITPNCYTISCLLDGLCKDGKISDALELFTEKI-EFQTPRCNVDAGGSKPSLTNHVAYTAL 540
           +  N  T + L+ G C+ G+I +A EL T  I +  +P C              + YT +
Sbjct: 788 LLANTATYNMLIRGFCRQGRIEEASELITRMIGDGVSPDC--------------ITYTTM 847

Query: 541 IHGLCQDGQFSKAVKLFSDMRRYGLQPDEVIYVVMLRGLFQVKYI--LMMLHADMLKFGV 596
           I+ LC+     KA++L++ M   G++PD V Y  ++ G      +     L  +ML+ G+
Sbjct: 848 INELCRRNDVKKAIELWNSMTEKGIRPDRVAYNTLIHGCCVAGEMGKATELRNEMLRQGL 892

HSP 6 Score: 232.3 bits (591), Expect = 8.3e-61
Identity = 167/631 (26.47%), Postives = 300/631 (47.54%), Query Frame = 1

Query: 11  TPDSLITTVLNCRSPWKALEFFN----AAPEKNIQLYSAIIHVLVGSKLLSHARYLL--- 70
           T  +L+  ++  R    A+E FN         ++ +Y+ +I  L   K LS A+ ++   
Sbjct: 194 TLSALLHGLVKFRHFGLAMELFNDMVSVGIRPDVYIYTGVIRSLCELKDLSRAKEMIAHM 253

Query: 71  ------------NDLVQNLVKSHKPYHACQLAFSELSRLKSSKFTPNV--YGELIIVLCK 130
                       N L+  L K  K + A  +       L      P+V  Y  L+  LCK
Sbjct: 254 EATGCDVNIVPYNVLIDGLCKKQKVWEAVGIK----KDLAGKDLKPDVVTYCTLVYGLCK 313

Query: 131 MELVEEALSMYHKVGA---ALTIQACNVLLYVLVKTGRFELLWRIYEEMISNGLSPSVIT 190
           ++  E  L M  ++     + +  A + L+  L K G+ E    + + ++  G+SP++  
Sbjct: 314 VQEFEIGLEMMDEMLCLRFSPSEAAVSSLVEGLRKRGKIEEALNLVKRVVDFGVSPNLFV 373

Query: 191 FGTLIDGCCRQGDLLRAQEMFDEMRVKGIVPTVIVYTILIRGLCSDNKIEEAESMHRAMR 250
           +  LID  C+      A+ +FD M   G+ P  + Y+ILI   C   K++ A S    M 
Sbjct: 374 YNALIDSLCKGRKFHEAELLFDRMGKIGLRPNDVTYSILIDMFCRRGKLDTALSFLGEMV 433

Query: 251 EVGVYPNVYTYNTLMDGYCKLANAKQALRLYQDMLGEGLVPDVVTFGILIDGLCKFGEMK 310
           + G+  +VY YN+L++G+CK  +   A     +M+ + L P VVT+  L+ G C  G++ 
Sbjct: 434 DTGLKLSVYPYNSLINGHCKFGDISAAEGFMAEMINKKLEPTVVTYTSLMGGYCSKGKIN 493

Query: 311 AARNLFVNMIKFSVTPNIAVYNSLIDAYCKVGDVSEAMALFLELERFEVSPDVFTYSILI 370
            A  L+  M    + P+I  + +L+    + G + +A+ LF E+  + V P+  TY+++I
Sbjct: 494 KALRLYHEMTGKGIAPSIYTFTTLLSGLFRAGLIRDAVKLFNEMAEWNVKPNRVTYNVMI 553

Query: 371 RGLCSVSRTEEAGNIFEKMTKEGILANSVTYNSLIDGCCKEGKMDKALEICSQMTENGVE 430
            G C      +A    ++MT++GI+ ++ +Y  LI G C  G+  +A      + +   E
Sbjct: 554 EGYCEEGDMSKAFEFLKEMTEKGIVPDTYSYRPLIHGLCLTGQASEAKVFVDGLHKGNCE 613

Query: 431 PNVITFSTLIDGYCKIRNLQAAMGIYSEMVIKSLSPDVVTYTAMIDGHCKYGSMKEALKL 490
            N I ++ L+ G+C+   L+ A+ +  EMV + +  D+V Y  +IDG  K+   K    L
Sbjct: 614 LNEICYTGLLHGFCREGKLEEALSVCQEMVQRGVDLDLVCYGVLIDGSLKHKDRKLFFGL 673

Query: 491 YSDMLDNGITPNCYTISCLLDGLCKDGKISDALELFTEKIEFQTPRCNVDAGGSKPSLTN 550
             +M D G+ P+    + ++D   K G   +A  ++   I             ++  + N
Sbjct: 674 LKEMHDRGLKPDDVIYTSMIDAKSKTGDFKEAFGIWDLMI-------------NEGCVPN 733

Query: 551 HVAYTALIHGLCQDGQFSKAVKLFSDMRRYGLQPDEVIYVVMLRGLFQVKYIL---MMLH 610
            V YTA+I+GLC+ G  ++A  L S M+     P++V Y   L  L + +  +   + LH
Sbjct: 734 EVTYTAVINGLCKAGFVNEAEVLCSKMQPVSSVPNQVTYGCFLDILTKGEVDMQKAVELH 793

Query: 611 ADMLKFGVIPNSAVHVILCECYQESGFLKSA 615
             +LK G++ N+A + +L   +   G ++ A
Sbjct: 794 NAILK-GLLANTATYNMLIRGFCRQGRIEEA 806

BLAST of Cucsa.275720 vs. NCBI nr
Match: gi|778703158|ref|XP_011655325.1| (PREDICTED: pentatricopeptide repeat-containing protein At5g61400 [Cucumis sativus])

HSP 1 Score: 1229.5 bits (3180), Expect = 0.0e+00
Identity = 616/616 (100.00%), Postives = 616/616 (100.00%), Query Frame = 1

Query: 1   MLQVVSLSSLTPDSLITTVLNCRSPWKALEFFNAAPEKNIQLYSAIIHVLVGSKLLSHAR 60
           MLQVVSLSSLTPDSLITTVLNCRSPWKALEFFNAAPEKNIQLYSAIIHVLVGSKLLSHAR
Sbjct: 32  MLQVVSLSSLTPDSLITTVLNCRSPWKALEFFNAAPEKNIQLYSAIIHVLVGSKLLSHAR 91

Query: 61  YLLNDLVQNLVKSHKPYHACQLAFSELSRLKSSKFTPNVYGELIIVLCKMELVEEALSMY 120
           YLLNDLVQNLVKSHKPYHACQLAFSELSRLKSSKFTPNVYGELIIVLCKMELVEEALSMY
Sbjct: 92  YLLNDLVQNLVKSHKPYHACQLAFSELSRLKSSKFTPNVYGELIIVLCKMELVEEALSMY 151

Query: 121 HKVGAALTIQACNVLLYVLVKTGRFELLWRIYEEMISNGLSPSVITFGTLIDGCCRQGDL 180
           HKVGAALTIQACNVLLYVLVKTGRFELLWRIYEEMISNGLSPSVITFGTLIDGCCRQGDL
Sbjct: 152 HKVGAALTIQACNVLLYVLVKTGRFELLWRIYEEMISNGLSPSVITFGTLIDGCCRQGDL 211

Query: 181 LRAQEMFDEMRVKGIVPTVIVYTILIRGLCSDNKIEEAESMHRAMREVGVYPNVYTYNTL 240
           LRAQEMFDEMRVKGIVPTVIVYTILIRGLCSDNKIEEAESMHRAMREVGVYPNVYTYNTL
Sbjct: 212 LRAQEMFDEMRVKGIVPTVIVYTILIRGLCSDNKIEEAESMHRAMREVGVYPNVYTYNTL 271

Query: 241 MDGYCKLANAKQALRLYQDMLGEGLVPDVVTFGILIDGLCKFGEMKAARNLFVNMIKFSV 300
           MDGYCKLANAKQALRLYQDMLGEGLVPDVVTFGILIDGLCKFGEMKAARNLFVNMIKFSV
Sbjct: 272 MDGYCKLANAKQALRLYQDMLGEGLVPDVVTFGILIDGLCKFGEMKAARNLFVNMIKFSV 331

Query: 301 TPNIAVYNSLIDAYCKVGDVSEAMALFLELERFEVSPDVFTYSILIRGLCSVSRTEEAGN 360
           TPNIAVYNSLIDAYCKVGDVSEAMALFLELERFEVSPDVFTYSILIRGLCSVSRTEEAGN
Sbjct: 332 TPNIAVYNSLIDAYCKVGDVSEAMALFLELERFEVSPDVFTYSILIRGLCSVSRTEEAGN 391

Query: 361 IFEKMTKEGILANSVTYNSLIDGCCKEGKMDKALEICSQMTENGVEPNVITFSTLIDGYC 420
           IFEKMTKEGILANSVTYNSLIDGCCKEGKMDKALEICSQMTENGVEPNVITFSTLIDGYC
Sbjct: 392 IFEKMTKEGILANSVTYNSLIDGCCKEGKMDKALEICSQMTENGVEPNVITFSTLIDGYC 451

Query: 421 KIRNLQAAMGIYSEMVIKSLSPDVVTYTAMIDGHCKYGSMKEALKLYSDMLDNGITPNCY 480
           KIRNLQAAMGIYSEMVIKSLSPDVVTYTAMIDGHCKYGSMKEALKLYSDMLDNGITPNCY
Sbjct: 452 KIRNLQAAMGIYSEMVIKSLSPDVVTYTAMIDGHCKYGSMKEALKLYSDMLDNGITPNCY 511

Query: 481 TISCLLDGLCKDGKISDALELFTEKIEFQTPRCNVDAGGSKPSLTNHVAYTALIHGLCQD 540
           TISCLLDGLCKDGKISDALELFTEKIEFQTPRCNVDAGGSKPSLTNHVAYTALIHGLCQD
Sbjct: 512 TISCLLDGLCKDGKISDALELFTEKIEFQTPRCNVDAGGSKPSLTNHVAYTALIHGLCQD 571

Query: 541 GQFSKAVKLFSDMRRYGLQPDEVIYVVMLRGLFQVKYILMMLHADMLKFGVIPNSAVHVI 600
           GQFSKAVKLFSDMRRYGLQPDEVIYVVMLRGLFQVKYILMMLHADMLKFGVIPNSAVHVI
Sbjct: 572 GQFSKAVKLFSDMRRYGLQPDEVIYVVMLRGLFQVKYILMMLHADMLKFGVIPNSAVHVI 631

Query: 601 LCECYQESGFLKSAQN 617
           LCECYQESGFLKSAQN
Sbjct: 632 LCECYQESGFLKSAQN 647

BLAST of Cucsa.275720 vs. NCBI nr
Match: gi|659127196|ref|XP_008463574.1| (PREDICTED: pentatricopeptide repeat-containing protein At5g61400 [Cucumis melo])

HSP 1 Score: 1166.4 bits (3016), Expect = 0.0e+00
Identity = 584/616 (94.81%), Postives = 600/616 (97.40%), Query Frame = 1

Query: 1   MLQVVSLSSLTPDSLITTVLNCRSPWKALEFFNAAPEKNIQLYSAIIHVLVGSKLLSHAR 60
           MLQVVSLSSLT DSL+TTVLNCRSP KALEFFNAAPEK IQLYSAIIHVLVGS+LLSHAR
Sbjct: 21  MLQVVSLSSLTSDSLLTTVLNCRSPRKALEFFNAAPEKTIQLYSAIIHVLVGSELLSHAR 80

Query: 61  YLLNDLVQNLVKSHKPYHACQLAFSELSRLKSSKFTPNVYGELIIVLCKMELVEEALSMY 120
           YLL DLVQNLVKSHKPYHACQL FSELSRLKSSKF+PNVYGELIIVLCKMELVEEALSMY
Sbjct: 81  YLLKDLVQNLVKSHKPYHACQLVFSELSRLKSSKFSPNVYGELIIVLCKMELVEEALSMY 140

Query: 121 HKVGAALTIQACNVLLYVLVKTGRFELLWRIYEEMISNGLSPSVITFGTLIDGCCRQGDL 180
           HKVGA LTIQACNVLL VLVKTGRFELLWRIYEEMISNGLSPSVITFGTLIDGCCRQGDL
Sbjct: 141 HKVGATLTIQACNVLLNVLVKTGRFELLWRIYEEMISNGLSPSVITFGTLIDGCCRQGDL 200

Query: 181 LRAQEMFDEMRVKGIVPTVIVYTILIRGLCSDNKIEEAESMHRAMREVGVYPNVYTYNTL 240
           LRAQEMFDEMRVKGIVPTV+VYTILIRGLCSD+K+EEAESMHRAMREVGVYPN+YTYNTL
Sbjct: 201 LRAQEMFDEMRVKGIVPTVVVYTILIRGLCSDSKMEEAESMHRAMREVGVYPNLYTYNTL 260

Query: 241 MDGYCKLANAKQALRLYQDMLGEGLVPDVVTFGILIDGLCKFGEMKAARNLFVNMIKFSV 300
           MDGYCKLANAKQALRLYQDMLGEGLVPDVVTFGILIDGLCKFGEMKAARNLFVNMIKF V
Sbjct: 261 MDGYCKLANAKQALRLYQDMLGEGLVPDVVTFGILIDGLCKFGEMKAARNLFVNMIKFCV 320

Query: 301 TPNIAVYNSLIDAYCKVGDVSEAMALFLELERFEVSPDVFTYSILIRGLCSVSRTEEAGN 360
           TPNI VYNSLIDAYCKVGDVSEAMA FLELER++VSPDVFTYSILIRGLCSV+RTEEAGN
Sbjct: 321 TPNINVYNSLIDAYCKVGDVSEAMAFFLELERYKVSPDVFTYSILIRGLCSVTRTEEAGN 380

Query: 361 IFEKMTKEGILANSVTYNSLIDGCCKEGKMDKALEICSQMTENGVEPNVITFSTLIDGYC 420
           IFEKMTKEGILANSVTYNSLIDG CKEGKM+KALEICSQMTENGVEPNVITFSTLIDGYC
Sbjct: 381 IFEKMTKEGILANSVTYNSLIDGYCKEGKMEKALEICSQMTENGVEPNVITFSTLIDGYC 440

Query: 421 KIRNLQAAMGIYSEMVIKSLSPDVVTYTAMIDGHCKYGSMKEALKLYSDMLDNGITPNCY 480
           KIRNLQAAMGIYSEMVIKSLSPDVVTYTAMIDGHCKYGSMKEALKLYSDMLDNGITPNCY
Sbjct: 441 KIRNLQAAMGIYSEMVIKSLSPDVVTYTAMIDGHCKYGSMKEALKLYSDMLDNGITPNCY 500

Query: 481 TISCLLDGLCKDGKISDALELFTEKIEFQTPRCNVDAGGSKPSLTNHVAYTALIHGLCQD 540
           TISCLLDGLCKDG+ISDAL LFTEKIEFQTPRCNVDAGGSKPSLTNHVAYTALIHGLCQD
Sbjct: 501 TISCLLDGLCKDGRISDALRLFTEKIEFQTPRCNVDAGGSKPSLTNHVAYTALIHGLCQD 560

Query: 541 GQFSKAVKLFSDMRRYGLQPDEVIYVVMLRGLFQVKYILMMLHADMLKFGVIPNSAVHVI 600
           GQF KAVKLFSDMRRYGLQPDEVIYVVML+GL QVK+ILMMLHADMLKFG IPNSAV+VI
Sbjct: 561 GQFFKAVKLFSDMRRYGLQPDEVIYVVMLQGLLQVKHILMMLHADMLKFGFIPNSAVYVI 620

Query: 601 LCECYQESGFLKSAQN 617
           LC+CYQ SGFLKSAQN
Sbjct: 621 LCKCYQGSGFLKSAQN 636

BLAST of Cucsa.275720 vs. NCBI nr
Match: gi|1009132528|ref|XP_015883421.1| (PREDICTED: pentatricopeptide repeat-containing protein At5g61400 [Ziziphus jujuba])

HSP 1 Score: 727.6 bits (1877), Expect = 1.8e-206
Identity = 368/620 (59.35%), Postives = 462/620 (74.52%), Query Frame = 1

Query: 5   VSLSSLTPDSLITTVLNCRSPWKALEFFNAA-----PEKNIQLYSAIIHVLVGSKLLSHA 64
           VS SS + + L  T+LNC++P +ALE FN A     P KN QLYSAI+H LVG+KL   A
Sbjct: 24  VSSSSSSSNDLTNTILNCKTPRQALESFNFAINQIGPRKNPQLYSAIVHFLVGAKLYCKA 83

Query: 65  RYLLNDLVQNLVKSHKPYHACQLAFSELSRLKSSKFTPNVYGELIIVLCKMELVEEALSM 124
           RYLL DL+  L K  KP  AC L F+ LSRL+SS+FTPNV+G LII L +M LV+E L +
Sbjct: 84  RYLLKDLILELQKFCKPRRACHLTFNALSRLESSRFTPNVFGSLIIALSEMGLVDEGLWV 143

Query: 125 YHKVGAALTIQACNVLLYVLVKTGRFELLWRIYEEMISNGLSPSVITFGTLIDGCCRQGD 184
           YHK+GA   IQACN LL  LV+  RF+ +W +Y EM S G SP+V+++G LID CC++GD
Sbjct: 144 YHKIGALPAIQACNALLGGLVEVARFDSMWELYREMGSRGFSPNVVSYGVLIDCCCKKGD 203

Query: 185 LLRAQEMFDEMRVKGIVPTVIVYTILIRGLCSDNKIEEAESMHRAMREVGVYPNVYTYNT 244
           +L A+E+FDEM  KGI PTV++YT LI GLCS +K+ EAESM  AMRE GV PN+YTYN+
Sbjct: 204 VLHARELFDEMGDKGIYPTVVIYTTLIHGLCSKSKMVEAESMFEAMREAGVLPNLYTYNS 263

Query: 245 LMDGYCKLANAKQALRLYQDMLGEGLVPDVVTFGILIDGLCKFGEMKAARNLFVNMIKFS 304
           L+DGYCKLAN KQAL LY++ML +G+ P+VVTFGIL+DGLCK      ARN F +M KF 
Sbjct: 264 LIDGYCKLANIKQALALYRNMLDDGVRPNVVTFGILVDGLCKVNIFTTARNFFASMAKFG 323

Query: 305 VTPNIAVYNSLIDAYCKVGDVSEAMALFLELERFEVSPDVFTYSILIRGLCSVSRTEEAG 364
           V PNI VYN LID +CK   + EAM  +LE+E+  + PDVFTY+ILI+GLC V R EEA 
Sbjct: 324 VRPNIFVYNCLIDGHCKAEKLYEAMEFYLEMEKHGIPPDVFTYNILIKGLCVVGRVEEAN 383

Query: 365 NIFEKMTKEGILANSVTYNSLIDGCCKEGKMDKALEICSQMTENGVEPNVITFSTLIDGY 424
            + +KM +EG++ANSVTYNSLIDG CKEG ++KALE+CSQMTENGVEPNVITFSTLIDGY
Sbjct: 384 GLLQKMNEEGVIANSVTYNSLIDGYCKEGNLEKALEVCSQMTENGVEPNVITFSTLIDGY 443

Query: 425 CKIRNLQAAMGIYSEMVIKSLSPDVVTYTAMIDGHCKYGSMKEALKLYSDMLDNGITPNC 484
           CK  N+ AAMG+YSEMVIK L PDVV +TA+IDGHCK  +MKEAL+L  +ML+ G+TPN 
Sbjct: 444 CKTGNMNAAMGMYSEMVIKGLLPDVVAFTALIDGHCKNNNMKEALRLQKEMLEVGLTPNL 503

Query: 485 YTISCLLDGLCKDGKISDALELFTEKIEFQTPRCNVDAGGSKPS----LTNHVAYTALIH 544
            T+SCL+DGL KDG+ SDA++LF EK    T    + + GSK        +HV YTA+I 
Sbjct: 504 LTVSCLIDGLFKDGRTSDAIKLFLEK----TRSNPLISEGSKSDCCFCFPDHVLYTAVIQ 563

Query: 545 GLCQDGQFSKAVKLFSDMRRYGLQPDEVIYVVMLRGLFQVKYIL--MMLHADMLKFGVIP 604
           GLC+DGQ  KA K FSDMR YGL+PD + Y+V+L+G FQ K+ L  M+LHADM+K G++P
Sbjct: 564 GLCKDGQIFKATKFFSDMRCYGLRPDVLTYIVILKGQFQAKHKLNVMLLHADMIKIGIMP 623

Query: 605 NSAVHVILCECYQESGFLKS 614
           N+ + +IL   Y+ +  LKS
Sbjct: 624 NAVLDLILTRGYRANVELKS 639

BLAST of Cucsa.275720 vs. NCBI nr
Match: gi|359491317|ref|XP_003634263.1| (PREDICTED: pentatricopeptide repeat-containing protein At5g61400 [Vitis vinifera])

HSP 1 Score: 707.6 bits (1825), Expect = 1.9e-200
Identity = 351/616 (56.98%), Postives = 459/616 (74.51%), Query Frame = 1

Query: 6   SLSSLTPDSLITTVLNCRSPWKALEFFNAAPE-----KNIQLYSAIIHVLVGSKLLSHAR 65
           S S  +P SL  ++L CR+  +ALE F++        KN QLYSAIIHVL G+KL + AR
Sbjct: 31  SSSDSSPSSLPNSILTCRTANQALELFHSVSRRADLAKNPQLYSAIIHVLTGAKLYAKAR 90

Query: 66  YLLNDLVQNLVKSHKPYHACQLAFSELSRLKSSKFTPNVYGELIIVLCKMELVEEALSMY 125
            L+ DL+Q L  S +    C + F+ LSRL+SSKFTPNV+G LII   +M LVEEAL +Y
Sbjct: 91  CLMRDLIQCLQNSRRSRICCSV-FNVLSRLESSKFTPNVFGVLIIAFSEMGLVEEALWVY 150

Query: 126 HKVGAALTIQACNVLLYVLVKTGRFELLWRIYEEMISNGLSPSVITFGTLIDGCCRQGDL 185
           +K+     +QACN++L  LVK GRF+ +W++Y +M++ G SP+V+T+GTLIDGCCRQGD 
Sbjct: 151 YKMDVLPAMQACNMVLDGLVKKGRFDTMWKVYGDMVARGASPNVVTYGTLIDGCCRQGDF 210

Query: 186 LRAQEMFDEMRVKGIVPTVIVYTILIRGLCSDNKIEEAESMHRAMREVGVYPNVYTYNTL 245
           L+A  +FDEM  K I PTV++YTILIRGLC +++I EAESM R MR  G+ PN+YTYNT+
Sbjct: 211 LKAFRLFDEMIEKKIFPTVVIYTILIRGLCGESRISEAESMFRTMRNSGMLPNLYTYNTM 270

Query: 246 MDGYCKLANAKQALRLYQDMLGEGLVPDVVTFGILIDGLCKFGEMKAARNLFVNMIKFSV 305
           MDGYCK+A+ K+AL LYQ+MLG+GL+P+VVTFGILIDGLCK  EM +AR   ++M  F V
Sbjct: 271 MDGYCKIAHVKKALELYQEMLGDGLLPNVVTFGILIDGLCKTDEMVSARKFLIDMASFGV 330

Query: 306 TPNIAVYNSLIDAYCKVGDVSEAMALFLELERFEVSPDVFTYSILIRGLCSVSRTEEAGN 365
            PNI VYN LID YCK G++SEA++L  E+E+ E+ PDVFTYSILI+GLC V R EEA  
Sbjct: 331 VPNIFVYNCLIDGYCKAGNLSEALSLHSEIEKHEILPDVFTYSILIKGLCGVDRMEEADG 390

Query: 366 IFEKMTKEGILANSVTYNSLIDGCCKEGKMDKALEICSQMTENGVEPNVITFSTLIDGYC 425
           + ++M K+G L N+VTYN+LIDG CKEG M+KA+E+CSQMTE G+EPN+ITFSTLIDGYC
Sbjct: 391 LLQEMKKKGFLPNAVTYNTLIDGYCKEGNMEKAIEVCSQMTEKGIEPNIITFSTLIDGYC 450

Query: 426 KIRNLQAAMGIYSEMVIKSLSPDVVTYTAMIDGHCKYGSMKEALKLYSDMLDNGITPNCY 485
           K   ++AAMG+Y+EMVIK L PDVV YTA+IDGH K G+ KEA +L+ +M + G+ PN +
Sbjct: 451 KAGKMEAAMGLYTEMVIKGLLPDVVAYTALIDGHFKDGNTKEAFRLHKEMQEAGLHPNVF 510

Query: 486 TISCLLDGLCKDGKISDALELFTEKIEFQTPRCNVDAGGSKPSLTNHVAYTALIHGLCQD 545
           T+SCL+DGLCKDG+ISDA++LF  K    T     +         NHV YTALI GLC D
Sbjct: 511 TLSCLIDGLCKDGRISDAIKLFLAKTGTDTTGSKTNELDRSLCSPNHVMYTALIQGLCTD 570

Query: 546 GQFSKAVKLFSDMRRYGLQPDEVIYVVMLRGLFQVKYI--LMMLHADMLKFGVIPNSAVH 605
           G+  KA K FSDMR  GL+PD    +V+++G F+  ++  +MML AD+LK G+IPNS+V+
Sbjct: 571 GRIFKASKFFSDMRCSGLRPDVFTCIVIIQGHFRAMHLRDVMMLQADILKMGIIPNSSVY 630

Query: 606 VILCECYQESGFLKSA 615
            +L + Y+ESG+LKSA
Sbjct: 631 RVLAKGYEESGYLKSA 645

BLAST of Cucsa.275720 vs. NCBI nr
Match: gi|147817754|emb|CAN66662.1| (hypothetical protein VITISV_031722 [Vitis vinifera])

HSP 1 Score: 707.2 bits (1824), Expect = 2.5e-200
Identity = 351/616 (56.98%), Postives = 459/616 (74.51%), Query Frame = 1

Query: 6   SLSSLTPDSLITTVLNCRSPWKALEFFNAAPE-----KNIQLYSAIIHVLVGSKLLSHAR 65
           S S  +P SL  ++L CR+  +ALE F++        KN QLYSAIIHVL G+KL + AR
Sbjct: 31  SSSDSSPSSLPNSILTCRTANQALELFHSVSRRADLAKNPQLYSAIIHVLTGAKLYAKAR 90

Query: 66  YLLNDLVQNLVKSHKPYHACQLAFSELSRLKSSKFTPNVYGELIIVLCKMELVEEALSMY 125
            L+ DL+Q L KS +    C + F+ LSRL+SSKFTPNV+G LII   +M LVEEAL +Y
Sbjct: 91  CLMRDLIQCLQKSRRSRICCSV-FNVLSRLESSKFTPNVFGVLIIAFSEMGLVEEALWVY 150

Query: 126 HKVGAALTIQACNVLLYVLVKTGRFELLWRIYEEMISNGLSPSVITFGTLIDGCCRQGDL 185
           +K+     +QACN++L  LVK GRF+ +W++Y +M++ G SP+V+T+GTLIDGCCRQGD 
Sbjct: 151 YKMDVLPAMQACNMVLDGLVKKGRFDTMWKVYGDMVARGASPNVVTYGTLIDGCCRQGDF 210

Query: 186 LRAQEMFDEMRVKGIVPTVIVYTILIRGLCSDNKIEEAESMHRAMREVGVYPNVYTYNTL 245
           L+A  +FDEM  K I PTV++YTILIRGLC +++I EAESM R MR  G+ PN+YTYNT+
Sbjct: 211 LKAFRLFDEMIEKKIFPTVVIYTILIRGLCGESRISEAESMFRTMRNSGMLPNLYTYNTM 270

Query: 246 MDGYCKLANAKQALRLYQDMLGEGLVPDVVTFGILIDGLCKFGEMKAARNLFVNMIKFSV 305
           MDGYCK+A+ K+AL LY +MLG+GL+P+VVTFGILIDGLCK  EM +AR   ++M  F V
Sbjct: 271 MDGYCKIAHVKKALELYXEMLGDGLLPNVVTFGILIDGLCKTDEMVSARKFLIDMASFGV 330

Query: 306 TPNIAVYNSLIDAYCKVGDVSEAMALFLELERFEVSPDVFTYSILIRGLCSVSRTEEAGN 365
            PNI VYN LID YCK G++SEA++L  E+E+ E+ PDVFTYSILI+GLC V R EEA  
Sbjct: 331 VPNIFVYNCLIDGYCKAGNLSEALSLHSEIEKHEILPDVFTYSILIKGLCGVDRMEEADG 390

Query: 366 IFEKMTKEGILANSVTYNSLIDGCCKEGKMDKALEICSQMTENGVEPNVITFSTLIDGYC 425
           + ++M K+G L N+VTYN+LIDG CKEG M+KA+E+CSQMTE G+EPN+ITFSTLIDGYC
Sbjct: 391 LLQEMKKKGFLPNAVTYNTLIDGYCKEGNMEKAIEVCSQMTEKGIEPNIITFSTLIDGYC 450

Query: 426 KIRNLQAAMGIYSEMVIKSLSPDVVTYTAMIDGHCKYGSMKEALKLYSDMLDNGITPNCY 485
           K   ++AAMG+Y+EMVIK L PDVV YTA+IDGH K G+ KEA +L+ +M + G+ PN +
Sbjct: 451 KAGKMEAAMGLYTEMVIKGLLPDVVAYTALIDGHFKDGNTKEAFRLHKEMQEAGLHPNVF 510

Query: 486 TISCLLDGLCKDGKISDALELFTEKIEFQTPRCNVDAGGSKPSLTNHVAYTALIHGLCQD 545
           T+SCL+DGLCKDG+ISDA++LF  K    T     +         NHV YTALI GLC D
Sbjct: 511 TLSCLIDGLCKDGRISDAIKLFLAKTGTDTTGSKTNELDRSLCSPNHVMYTALIQGLCTD 570

Query: 546 GQFSKAVKLFSDMRRYGLQPDEVIYVVMLRGLFQVKYI--LMMLHADMLKFGVIPNSAVH 605
           G+  KA K FSDMR  GL+PD    +V+++G F+  ++  +MML AD+LK G+IPNS+V+
Sbjct: 571 GRIFKASKFFSDMRCSGLRPDVFTCIVIIQGHFRAMHLRDVMMLQADILKMGIIPNSSVY 630

Query: 606 VILCECYQESGFLKSA 615
            +L + Y+ESG+LKSA
Sbjct: 631 RVLAKGYEESGYLKSA 645