Cucsa.257610 (gene) Cucumber (Gy14) v1

BLAST of Cucsa.257610 vs. Swiss-Prot
Match: PP202_ARATH (Pentatricopeptide repeat-containing protein At2g45350, chloroplastic OS=Arabidopsis thaliana GN=CRR4 PE=1 SV=2)

HSP 1 Score: 628.6 bits (1620), Expect = 5.9e-179
Identity = 302/521 (57.97%), Postives = 388/521 (74.47%), Query Frame = 1

Query: 8   DDPFLWNAVIKSYSHGNEPVRALL------------------------------------ 67
           +DPFLWNAVIKS+SHG +P +ALL                                    
Sbjct: 84  EDPFLWNAVIKSHSHGKDPRQALLLLCLMLENGVSVDKFSLSLVLKACSRLGFVKGGMQI 143

Query: 68  -------EIGSNLFLLNCLIAMYLRCGDIEFARQVFDRMPIQDSVSYNSMIDGYVKSGTI 127
                   + S+LFL NCLI +YL+CG +  +RQ+FDRMP +DSVSYNSMIDGYVK G I
Sbjct: 144 HGFLKKTGLWSDLFLQNCLIGLYLKCGCLGLSRQMFDRMPKRDSVSYNSMIDGYVKCGLI 203

Query: 128 DLARELFDSMPLEDKNLISWNSMLGGFAQTKDGIGLALELFEKMPERDLVSWNTIIGGFA 187
             ARELFD MP+E KNLISWNSM+ G+AQT DG+ +A +LF  MPE+DL+SWN++I G+ 
Sbjct: 204 VSARELFDLMPMEMKNLISWNSMISGYAQTSDGVDIASKLFADMPEKDLISWNSMIDGYV 263

Query: 188 KCGRIEFAHSLFNRMPKRDVISWSNMIDGYAKLGDIKVARTLFDEMPDKDVVAFNTIMAG 247
           K GRIE A  LF+ MP+RDV++W+ MIDGYAKLG +  A+TLFD+MP +DVVA+N++MAG
Sbjct: 264 KHGRIEDAKGLFDVMPRRDVVTWATMIDGYAKLGFVHHAKTLFDQMPHRDVVAYNSMMAG 323

Query: 248 YAQNGYYTEALEIFHEMQRQSNLSPDETTLVVALSAISQLGHVEKAASMHNYFLENGISV 307
           Y QN Y+ EALEIF +M+++S+L PD+TTLV+ L AI+QLG + KA  MH Y +E    +
Sbjct: 324 YVQNKYHMEALEIFSDMEKESHLLPDDTTLVIVLPAIAQLGRLSKAIDMHLYIVEKQFYL 383

Query: 308 TGKVAVALIDMYSKCGSIENAILIFDGVDQKGIDHWNAMISGMARNGLGKLAFGMLLEMH 367
            GK+ VALIDMYSKCGSI++A+L+F+G++ K IDHWNAMI G+A +GLG+ AF MLL++ 
Sbjct: 384 GGKLGVALIDMYSKCGSIQHAMLVFEGIENKSIDHWNAMIGGLAIHGLGESAFDMLLQIE 443

Query: 368 RLSVKPDGITFIGVLNACAHAGLVKEGLICFELMRKVHKLEPKLQHYGCMVDILGKAGLV 427
           RLS+KPD ITF+GVLNAC+H+GLVKEGL+CFELMR+ HK+EP+LQHYGCMVDIL ++G +
Sbjct: 444 RLSLKPDDITFVGVLNACSHSGLVKEGLLCFELMRRKHKIEPRLQHYGCMVDILSRSGSI 503

Query: 428 EGALKFIEEMPIEPNDIIWRTLLSACQNHENFTIGELIAKHLMTMDSCNSSSYVLLSNIY 486
           E A   IEEMP+EPND+IWRT L+AC +H+ F  GEL+AKHL+     N SSYVLLSN+Y
Sbjct: 504 ELAKNLIEEMPVEPNDVIWRTFLTACSHHKEFETGELVAKHLILQAGYNPSSYVLLSNMY 563

BLAST of Cucsa.257610 vs. Swiss-Prot
Match: PP175_ARATH (Pentatricopeptide repeat-containing protein At2g29760, chloroplastic OS=Arabidopsis thaliana GN=PCMP-H33 PE=2 SV=1)

HSP 1 Score: 371.7 bits (953), Expect = 1.3e-101
Identity = 190/473 (40.17%), Postives = 291/473 (61.52%), Query Frame = 1

Query: 33  IGSNLFLLNCLIAMYLRCGDIEFARQVFDRMPIQDSVSYNSMIDGYVKSGTIDLARELFD 92
           +GS++F+ N LI  Y  CGD++ A +VF  +  +D VS+NSMI+G+V+ G+ D A ELF 
Sbjct: 162 VGSDVFVANSLIHCYFSCGDLDSACKVFTTIKEKDVVSWNSMINGFVQKGSPDKALELFK 221

Query: 93  SMPLEDKNL--ISWNSMLGGFAQTKD---GIGLALELFEKMPERDLVSWNTIIGGFAKCG 152
            M  ED     ++   +L   A+ ++   G  +   + E     +L   N ++  + KCG
Sbjct: 222 KMESEDVKASHVTMVGVLSACAKIRNLEFGRQVCSYIEENRVNVNLTLANAMLDMYTKCG 281

Query: 153 RIEFAHSLFNRMPKRDVISWSNMIDGYAKLGDIKVARTLFDEMPDKDVVAFNTIMAGYAQ 212
            IE A  LF+ M ++D ++W+ M+DGYA   D + AR + + MP KD+VA+N +++ Y Q
Sbjct: 282 SIEDAKRLFDAMEEKDNVTWTTMLDGYAISEDYEAAREVLNSMPQKDIVAWNALISAYEQ 341

Query: 213 NGYYTEALEIFHEMQRQSNLSPDETTLVVALSAISQLGHVEKAASMHNYFLENGISVTGK 272
           NG   EAL +FHE+Q Q N+  ++ TLV  LSA +Q+G +E    +H+Y  ++GI +   
Sbjct: 342 NGKPNEALIVFHELQLQKNMKLNQITLVSTLSACAQVGALELGRWIHSYIKKHGIRMNFH 401

Query: 273 VAVALIDMYSKCGSIENAILIFDGVDQKGIDHWNAMISGMARNGLGKLAFGMLLEMHRLS 332
           V  ALI MYSKCG +E +  +F+ V+++ +  W+AMI G+A +G G  A  M  +M   +
Sbjct: 402 VTSALIHMYSKCGDLEKSREVFNSVEKRDVFVWSAMIGGLAMHGCGNEAVDMFYKMQEAN 461

Query: 333 VKPDGITFIGVLNACAHAGLVKEGLICFELMRKVHKLEPKLQHYGCMVDILGKAGLVEGA 392
           VKP+G+TF  V  AC+H GLV E    F  M   + + P+ +HY C+VD+LG++G +E A
Sbjct: 462 VKPNGVTFTNVFCACSHTGLVDEAESLFHQMESNYGIVPEEKHYACIVDVLGRSGYLEKA 521

Query: 393 LKFIEEMPIEPNDIIWRTLLSACQNHENFTIGELIAKHLMTMDSCNSSSYVLLSNIYARL 452
           +KFIE MPI P+  +W  LL AC+ H N  + E+    L+ ++  N  ++VLLSNIYA+L
Sbjct: 522 VKFIEAMPIPPSTSVWGALLGACKIHANLNLAEMACTRLLELEPRNDGAHVLLSNIYAKL 581

Query: 453 GLWSAASKVRMMMKKQNLTKVPGCSWIELEGVVHEFLVRDKSHPYVSEIYSVL 501
           G W   S++R  M+   L K PGCS IE++G++HEFL  D +HP   ++Y  L
Sbjct: 582 GKWENVSELRKHMRVTGLKKEPGCSSIEIDGMIHEFLSGDNAHPMSEKVYGKL 634

HSP 2 Score: 108.6 bits (270), Expect = 2.1e-22
Identity = 76/281 (27.05%), Postives = 144/281 (51.25%), Query Frame = 1

Query: 83  TIDLARELFDSMPLEDKNLISWNSMLGGFAQTKDGIGLALELFEKMPERDLV----SWNT 142
           +++ AR++FD +P    N  +WN+++  +A   D +       + + E        ++  
Sbjct: 79  SLEYARKVFDEIP--KPNSFAWNTLIRAYASGPDPVLSIWAFLDMVSESQCYPNKYTFPF 138

Query: 143 IIGGFAKCGRIEFAHSLFNRMPKR----DVISWSNMIDGYAKLGDIKVARTLFDEMPDKD 202
           +I   A+   +    SL     K     DV   +++I  Y   GD+  A  +F  + +KD
Sbjct: 139 LIKAAAEVSSLSLGQSLHGMAVKSAVGSDVFVANSLIHCYFSCGDLDSACKVFTTIKEKD 198

Query: 203 VVAFNTIMAGYAQNGYYTEALEIFHEMQRQSNLSPDETTLVVALSAISQLGHVEKAASMH 262
           VV++N+++ G+ Q G   +ALE+F +M+ + ++     T+V  LSA +++ ++E    + 
Sbjct: 199 VVSWNSMINGFVQKGSPDKALELFKKMESE-DVKASHVTMVGVLSACAKIRNLEFGRQVC 258

Query: 263 NYFLENGISVTGKVAVALIDMYSKCGSIENAILIFDGVDQKGIDHWNAMISGMARNGLGK 322
           +Y  EN ++V   +A A++DMY+KCGSIE+A  +FD +++K    W  M+ G A +   +
Sbjct: 259 SYIEENRVNVNLTLANAMLDMYTKCGSIEDAKRLFDAMEEKDNVTWTTMLDGYAISEDYE 318

Query: 323 LAFGMLLEMHRLSVKPDGITFIGVLNACAHAGLVKEGLICF 356
            A  +L  M     + D + +  +++A    G   E LI F
Sbjct: 319 AAREVLNSM----PQKDIVAWNALISAYEQNGKPNEALIVF 352

HSP 3 Score: 102.4 bits (254), Expect = 1.5e-20
Identity = 61/243 (25.10%), Postives = 121/243 (49.79%), Query Frame = 1

Query: 175 AKLGDIKVARTLFDEMPDKDVVAFNTIMAGYAQNGYYTEALEIFHEMQRQSNLSPDETTL 234
           +    ++ AR +FDE+P  +  A+NT++  YA       ++  F +M  +S   P++ T 
Sbjct: 75  SSFASLEYARKVFDEIPKPNSFAWNTLIRAYASGPDPVLSIWAFLDMVSESQCYPNKYTF 134

Query: 235 VVALSAISQLGHVEKAASMHNYFLENGISVTGKVAVALIDMYSKCGSIENAILIFDGVDQ 294
              + A +++  +    S+H   +++ +     VA +LI  Y  CG +++A  +F  + +
Sbjct: 135 PFLIKAAAEVSSLSLGQSLHGMAVKSAVGSDVFVANSLIHCYFSCGDLDSACKVFTTIKE 194

Query: 295 KGIDHWNAMISGMARNGLGKLAFGMLLEMHRLSVKPDGITFIGVLNACAHAGLVKEG-LI 354
           K +  WN+MI+G  + G    A  +  +M    VK   +T +GVL+ACA    ++ G  +
Sbjct: 195 KDVVSWNSMINGFVQKGSPDKALELFKKMESEDVKASHVTMVGVLSACAKIRNLEFGRQV 254

Query: 355 CFELMRKVHKLEPKLQHYGCMVDILGKAGLVEGALKFIEEMPIEPNDIIWRTLLSACQNH 414
           C  +  + +++   L     M+D+  K G +E A +  + M  E +++ W T+L      
Sbjct: 255 CSYI--EENRVNVNLTLANAMLDMYTKCGSIEDAKRLFDAME-EKDNVTWTTMLDGYAIS 314

Query: 415 ENF 417
           E++
Sbjct: 315 EDY 314

HSP 4 Score: 99.0 bits (245), Expect = 1.6e-19
Identity = 91/390 (23.33%), Postives = 177/390 (45.38%), Query Frame = 1

Query: 35  SNLFLLNCLIAMYLRCGDIEFARQVFDRMPIQDSVSYNSMIDGYVKSGTIDLARELFDSM 94
           S LF +  L +       +E+AR+VFD +P  +S ++N++I  Y       L+   F  M
Sbjct: 66  SKLFAMAALSSF----ASLEYARKVFDEIPKPNSFAWNTLIRAYASGPDPVLSIWAFLDM 125

Query: 95  PLEDK---NLISWNSMLGGFAQTKD-GIGLALE--LFEKMPERDLVSWNTIIGGFAKCGR 154
             E +   N  ++  ++   A+     +G +L     +     D+   N++I  +  CG 
Sbjct: 126 VSESQCYPNKYTFPFLIKAAAEVSSLSLGQSLHGMAVKSAVGSDVFVANSLIHCYFSCGD 185

Query: 155 IEFAHSLFNRMPKRDVISWSNMIDGYAKLGDIKVARTLFDEMPDKDVVAFNTIMAGYAQN 214
           ++ A  +F  + ++DV+SW++MI+G+ + G    A  LF +M  +DV A +  M G    
Sbjct: 186 LDSACKVFTTIKEKDVVSWNSMINGFVQKGSPDKALELFKKMESEDVKASHVTMVGVLSA 245

Query: 215 GYYTEALEIFHEM---QRQSNLSPDETTLVVALSAISQLGHVEKAASMHNYFLENGISVT 274
                 LE   ++     ++ ++ + T     L   ++ G +E A  + +  +E   +VT
Sbjct: 246 CAKIRNLEFGRQVCSYIEENRVNVNLTLANAMLDMYTKCGSIEDAKRLFD-AMEEKDNVT 305

Query: 275 GKVAVALIDMYSKCGSIENAILIFDGVDQKGIDHWNAMISGMARNGLGKLAFGMLLEMH- 334
                 ++D Y+     E A  + + + QK I  WNA+IS   +NG    A  +  E+  
Sbjct: 306 W---TTMLDGYAISEDYEAAREVLNSMPQKDIVAWNALISAYEQNGKPNEALIVFHELQL 365

Query: 335 RLSVKPDGITFIGVLNACAHAGLVKEGLICFELMRKVHKLEPKLQHYGCMVDILGKAGLV 394
           + ++K + IT +  L+ACA  G ++ G      ++K H +         ++ +  K G +
Sbjct: 366 QKNMKLNQITLVSTLSACAQVGALELGRWIHSYIKK-HGIRMNFHVTSALIHMYSKCGDL 425

Query: 395 EGALKFIEEMPIEPNDI-IWRTLLSACQNH 414
           E + +      +E  D+ +W  ++     H
Sbjct: 426 EKSREVFNS--VEKRDVFVWSAMIGGLAMH 444

BLAST of Cucsa.257610 vs. Swiss-Prot
Match: PP301_ARATH (Pentatricopeptide repeat-containing protein At4g02750 OS=Arabidopsis thaliana GN=PCMP-H24 PE=3 SV=1)

HSP 1 Score: 369.4 bits (947), Expect = 6.4e-101
Identity = 198/493 (40.16%), Postives = 297/493 (60.24%), Query Frame = 1

Query: 13  WNAVIKSYSHGNEPVRALLEIGSN----LFLLNCLIAMYLRCGDIEFARQVFDRMPIQDS 72
           WNA++ +Y   ++   A +   S     L   NCL+  +++   I  ARQ FD M ++D 
Sbjct: 191 WNALLSAYVQNSKMEEACMLFKSRENWALVSWNCLLGGFVKKKKIVEARQFFDSMNVRDV 250

Query: 73  VSYNSMIDGYVKSGTIDLARELFDSMPLEDKNLISWNSMLGGFAQTKDGIGLALELFEKM 132
           VS+N++I GY +SG ID AR+LFD  P++D  + +W +M+ G+ Q +  +  A ELF+KM
Sbjct: 251 VSWNTIITGYAQSGKIDEARQLFDESPVQD--VFTWTAMVSGYIQNRM-VEEARELFDKM 310

Query: 133 PERDLVSWNTIIGGFAKCGRIEFAHSLFNRMPKRDVISWSNMIDGYAKLGDIKVARTLFD 192
           PER+ VSWN ++ G+ +  R+E A  LF+ MP R+V +W+ MI GYA+ G I  A+ LFD
Sbjct: 311 PERNEVSWNAMLAGYVQGERMEMAKELFDVMPCRNVSTWNTMITGYAQCGKISEAKNLFD 370

Query: 193 EMPDKDVVAFNTIMAGYAQNGYYTEALEIFHEMQRQSNLSPDETTLVVALSAISQLGHVE 252
           +MP +D V++  ++AGY+Q+G+  EAL +F +M+R+     + ++   ALS  + +  +E
Sbjct: 371 KMPKRDPVSWAAMIAGYSQSGHSFEALRLFVQMEREGGRL-NRSSFSSALSTCADVVALE 430

Query: 253 KAASMHNYFLENGISVTGKVAVALIDMYSKCGSIENAILIFDGVDQKGIDHWNAMISGMA 312
               +H   ++ G      V  AL+ MY KCGSIE A  +F  +  K I  WN MI+G +
Sbjct: 431 LGKQLHGRLVKGGYETGCFVGNALLLMYCKCGSIEEANDLFKEMAGKDIVSWNTMIAGYS 490

Query: 313 RNGLGKLAFGMLLEMHRLSVKPDGITFIGVLNACAHAGLVKEGLICFELMRKVHKLEPKL 372
           R+G G++A      M R  +KPD  T + VL+AC+H GLV +G   F  M + + + P  
Sbjct: 491 RHGFGEVALRFFESMKREGLKPDDATMVAVLSACSHTGLVDKGRQYFYTMTQDYGVMPNS 550

Query: 373 QHYGCMVDILGKAGLVEGALKFIEEMPIEPNDIIWRTLLSACQNHENFTIGELIAKHLMT 432
           QHY CMVD+LG+AGL+E A   ++ MP EP+  IW TLL A + H N  + E  A  +  
Sbjct: 551 QHYACMVDLLGRAGLLEDAHNLMKNMPFEPDAAIWGTLLGASRVHGNTELAETAADKIFA 610

Query: 433 MDSCNSSSYVLLSNIYARLGLWSAASKVRMMMKKQNLTKVPGCSWIELEGVVHEFLVRDK 492
           M+  NS  YVLLSN+YA  G W    K+R+ M+ + + KVPG SWIE++   H F V D+
Sbjct: 611 MEPENSGMYVLLSNLYASSGRWGDVGKLRVRMRDKGVKKVPGYSWIEIQNKTHTFSVGDE 670

Query: 493 SHPYVSEIYSVLD 502
            HP   EI++ L+
Sbjct: 671 FHPEKDEIFAFLE 679

HSP 2 Score: 177.6 bits (449), Expect = 3.6e-43
Identity = 120/380 (31.58%), Postives = 197/380 (51.84%), Query Frame = 1

Query: 41  NCLIAMYLRCGDIEFARQVFDRMPIQDSVSYNSMIDGYVKSGTIDLARELFDSMPLEDKN 100
           N +I  Y+R  ++  AR++F+ MP +D  S+N+M+ GY ++G +D AR +FD MP  +KN
Sbjct: 130 NVMIKGYVRNRNLGKARELFEIMPERDVCSWNTMLSGYAQNGCVDDARSVFDRMP--EKN 189

Query: 101 LISWNSMLGGFAQTKDGIGLALELFEKMPERDLVSWNTIIGGFAKCGRIEFAHSLFNRMP 160
            +SWN++L  + Q    +  A  LF+      LVSWN ++GGF K  +I  A   F+ M 
Sbjct: 190 DVSWNALLSAYVQNSK-MEEACMLFKSRENWALVSWNCLLGGFVKKKKIVEARQFFDSMN 249

Query: 161 KRDVISWSNMIDGYAKLGDIKVARTLFDEMPDKDVVAFNTIMAGYAQNGYYTEALEIFHE 220
            RDV+SW+ +I GYA+ G I  AR LFDE P +DV  +  +++GY QN    EA E+F +
Sbjct: 250 VRDVVSWNTIITGYAQSGKIDEARQLFDESPVQDVFTWTAMVSGYIQNRMVEEARELFDK 309

Query: 221 MQRQSNLSPDETTLVVALSAISQLGHVEKAASMHNYFLENGISVTGKVAVALIDMYSKCG 280
           M  ++ +S +       L+   Q   +E A  + +      +S        +I  Y++CG
Sbjct: 310 MPERNEVSWN-----AMLAGYVQGERMEMAKELFDVMPCRNVSTWN----TMITGYAQCG 369

Query: 281 SIENAILIFDGVDQKGIDHWNAMISGMARNGLGKLAFGMLLEMHRLSVKPDGITFIGVLN 340
            I  A  +FD + ++    W AMI+G +++G    A  + ++M R   + +  +F   L+
Sbjct: 370 KISEAKNLFDKMPKRDPVSWAAMIAGYSQSGHSFEALRLFVQMEREGGRLNRSSFSSALS 429

Query: 341 ACAHAGLVKEGLICFELMRKVH-KLEPKLQHYGCMVD-----ILGKAGLVEGALKFIEEM 400
            CA        ++  EL +++H +L       GC V      +  K G +E A    +EM
Sbjct: 430 TCA-------DVVALELGKQLHGRLVKGGYETGCFVGNALLLMYCKCGSIEEANDLFKEM 488

Query: 401 PIEPNDII-WRTLLSACQNH 414
                DI+ W T+++    H
Sbjct: 490 --AGKDIVSWNTMIAGYSRH 488

HSP 3 Score: 165.6 bits (418), Expect = 1.4e-39
Identity = 118/359 (32.87%), Postives = 188/359 (52.37%), Query Frame = 1

Query: 7   DDDPFLWNAVIKSYSHG---NEPVRALLEIGS-NLFLLNCLIAMYLRCGDIEFARQVFDR 66
           D D   WN  I SY      NE +R    +   +    N +I+ YLR G+ E AR++FD 
Sbjct: 61  DSDIKEWNVAISSYMRTGRCNEALRVFKRMPRWSSVSYNGMISGYLRNGEFELARKLFDE 120

Query: 67  MPIQDSVSYNSMIDGYVKSGTIDLARELFDSMPLEDKNLISWNSMLGGFAQTKDGIGLAL 126
           MP +D VS+N MI GYV++  +  ARELF+ MP  ++++ SWN+ML G+AQ    +  A 
Sbjct: 121 MPERDLVSWNVMIKGYVRNRNLGKARELFEIMP--ERDVCSWNTMLSGYAQN-GCVDDAR 180

Query: 127 ELFEKMPERDLVSWNTIIGGFAKCGRIEFAHSLFNRMPKRDVISWSNMIDGYAKLGDIKV 186
            +F++MPE++ VSWN ++  + +  ++E A  LF       ++SW+ ++ G+ K   I  
Sbjct: 181 SVFDRMPEKNDVSWNALLSAYVQNSKMEEACMLFKSRENWALVSWNCLLGGFVKKKKIVE 240

Query: 187 ARTLFDEMPDKDVVAFNTIMAGYAQNGYYTEALEIFHEMQRQSNLSPDETTLVVALSAIS 246
           AR  FD M  +DVV++NTI+ GYAQ+G   EA ++F E   Q     D  T    +S   
Sbjct: 241 ARQFFDSMNVRDVVSWNTIITGYAQSGKIDEARQLFDESPVQ-----DVFTWTAMVSGYI 300

Query: 247 QLGHVEKAASMHNYFLE-NGISVTGKVAVALIDMYSKCGSIENAILIFDGVDQKGIDHWN 306
           Q   VE+A  + +   E N +S       A++  Y +   +E A  +FD +  + +  WN
Sbjct: 301 QNRMVEEARELFDKMPERNEVSWN-----AMLAGYVQGERMEMAKELFDVMPCRNVSTWN 360

Query: 307 AMISGMARNGLGKLAFGMLLEMHRLSVKPDGITFIGVLNACAHAGLVKEGLICFELMRK 361
            MI+G A+ G    A  +  +M     K D +++  ++   + +G   E L  F  M +
Sbjct: 361 TMITGYAQCGKISEAKNLFDKM----PKRDPVSWAAMIAGYSQSGHSFEALRLFVQMER 402

HSP 4 Score: 162.9 bits (411), Expect = 9.2e-39
Identity = 134/462 (29.00%), Postives = 226/462 (48.92%), Query Frame = 1

Query: 13  WNAVIKSYSHGNE--PVRALLEI--GSNLFLLNCLIAMYLRCGDIEFARQVFDRMPIQDS 72
           WN +IK Y         R L EI    ++   N +++ Y + G ++ AR VFDRMP ++ 
Sbjct: 129 WNVMIKGYVRNRNLGKARELFEIMPERDVCSWNTMLSGYAQNGCVDDARSVFDRMPEKND 188

Query: 73  VSYNSMIDGYVKSGTIDLARELFDSMPLEDKNLISWNSMLGGFAQTKDGIGLALELFEKM 132
           VS+N+++  YV++  ++ A  LF S   E+  L+SWN +LGGF + K  I  A + F+ M
Sbjct: 189 VSWNALLSAYVQNSKMEEACMLFKSR--ENWALVSWNCLLGGFVKKKK-IVEARQFFDSM 248

Query: 133 PERDLVSWNTIIGGFAKCGRIEFAHSLFNRMPKRDVISWSNMIDGYAKLGDIKVARTLFD 192
             RD+VSWNTII G+A+ G+I+ A  LF+  P +DV +W+ M+ GY +   ++ AR LFD
Sbjct: 249 NVRDVVSWNTIITGYAQSGKIDEARQLFDESPVQDVFTWTAMVSGYIQNRMVEEARELFD 308

Query: 193 EMPDKDVVAFNTIMAGYAQNGYYTEALEIFHEMQRQSNLSPDETTLVVALSAISQLGHVE 252
           +MP+++ V++N ++AGY Q      A E+F  M  +     + +T    ++  +Q G + 
Sbjct: 309 KMPERNEVSWNAMLAGYVQGERMEMAKELFDVMPCR-----NVSTWNTMITGYAQCGKIS 368

Query: 253 KAASMHNYFLENGISVTGKVAVALIDMYSKCGSIENAILIFDGVDQKGIDHWNAMISGMA 312
           +A ++ +   +           A+I  YS+ G    A+ +F  ++++G     +  S   
Sbjct: 369 EAKNLFDKMPKR----DPVSWAAMIAGYSQSGHSFEALRLFVQMEREGGRLNRSSFSSAL 428

Query: 313 RNGLGKLAFGMLLEMHRLSVKPDGIT--FIG--VLNACAHAGLVKEGLICFELMRKVHKL 372
                 +A  +  ++H   VK    T  F+G  +L      G ++E    F+ M      
Sbjct: 429 STCADVVALELGKQLHGRLVKGGYETGCFVGNALLLMYCKCGSIEEANDLFKEM-----A 488

Query: 373 EPKLQHYGCMVDILGKAGLVEGALKFIEEMP---IEPNDIIWRTLLSACQNHENFTIGEL 432
              +  +  M+    + G  E AL+F E M    ++P+D     +LSAC +H        
Sbjct: 489 GKDIVSWNTMIAGYSRHGFGEVALRFFESMKREGLKPDDATMVAVLSAC-SHTGLVDKGR 548

Query: 433 IAKHLMTMD---SCNSSSYVLLSNIYARLGLWSAASKVRMMM 461
              + MT D     NS  Y  + ++  R GL   A  +   M
Sbjct: 549 QYFYTMTQDYGVMPNSQHYACMVDLLGRAGLLEDAHNLMKNM 572

HSP 5 Score: 118.6 bits (296), Expect = 2.0e-25
Identity = 87/311 (27.97%), Postives = 157/311 (50.48%), Query Frame = 1

Query: 98  DKNLISWNSMLGGFAQTKDGIGLALELFEKMPERDLVSWNTIIGGFAKCGRIEFAHSLFN 157
           D ++  WN  +  + +T      AL +F++MP    VS+N +I G+ + G  E A  LF+
Sbjct: 61  DSDIKEWNVAISSYMRT-GRCNEALRVFKRMPRWSSVSYNGMISGYLRNGEFELARKLFD 120

Query: 158 RMPKRDVISWSNMIDGYAKLGDIKVARTLFDEMPDKDVVAFNTIMAGYAQNGYYTEALEI 217
            MP+RD++SW+ MI GY +  ++  AR LF+ MP++DV ++NT+++GYAQNG   +A  +
Sbjct: 121 EMPERDLVSWNVMIKGYVRNRNLGKARELFEIMPERDVCSWNTMLSGYAQNGCVDDARSV 180

Query: 218 FHEMQRQSNLSPDETTLVVALSAISQLGHVEKAASMHNYFLENGISVTGKVAVALIDMYS 277
           F  M  ++++S +       LSA  Q   +E+A  +     EN   V+      L+  + 
Sbjct: 181 FDRMPEKNDVSWN-----ALLSAYVQNSKMEEACMLFK-SRENWALVSWN---CLLGGFV 240

Query: 278 KCGSIENAILIFDGVDQKGIDHWNAMISGMARNGLGKLAFGMLLEMHRLSVKPDGITFIG 337
           K   I  A   FD ++ + +  WN +I+G A++G    A  +  E    S   D  T+  
Sbjct: 241 KKKKIVEARQFFDSMNVRDVVSWNTIITGYAQSGKIDEARQLFDE----SPVQDVFTWTA 300

Query: 338 VLNACAHAGLVKEGLICFELMRKVHKLEPKLQHYGCMVDILGKAGLVEGALKFIEEMPIE 397
           +++      +V+E    F+ M + +++      +  M+    +   +E A +  + MP  
Sbjct: 301 MVSGYIQNRMVEEARELFDKMPERNEVS-----WNAMLAGYVQGERMEMAKELFDVMPCR 351

Query: 398 PNDIIWRTLLS 409
            N   W T+++
Sbjct: 361 -NVSTWNTMIT 351

HSP 6 Score: 107.8 bits (268), Expect = 3.5e-22
Identity = 90/343 (26.24%), Postives = 161/343 (46.94%), Query Frame = 1

Query: 127 KMPERDLVSWNTIIGGFAKCGRIEFAHSLFNRMPKRDVISWSNMIDGYAKLGDIKVARTL 186
           K  + D+  WN  I  + + GR   A  +F RMP+   +S++ MI GY + G+ ++AR L
Sbjct: 58  KCGDSDIKEWNVAISSYMRTGRCNEALRVFKRMPRWSSVSYNGMISGYLRNGEFELARKL 117

Query: 187 FDEMPDKDVVAFNTIMAGYAQNGYYTEALEIFHEMQRQSNLSPDETTLVVALSAISQLGH 246
           FDEMP++D+V++N ++ GY +N    +A E+F  M  +     D  +    LS  +Q G 
Sbjct: 118 FDEMPERDLVSWNVMIKGYVRNRNLGKARELFEIMPER-----DVCSWNTMLSGYAQNGC 177

Query: 247 VEKAASMHNYFLE-NGISVTGKVAVALIDMYSKCGSIENAILIFDGVDQKGIDHWNAMIS 306
           V+ A S+ +   E N +S       AL+  Y +   +E A ++F   +   +  WN ++ 
Sbjct: 178 VDDARSVFDRMPEKNDVSWN-----ALLSAYVQNSKMEEACMLFKSRENWALVSWNCLLG 237

Query: 307 GMARNGLGKLAFGMLLEMHRLSVKPDGITFIGVLNACAHAGLVKEGLICFELMRKVHKLE 366
           G  +    K           ++V+ D +++  ++   A +G + E    F+        E
Sbjct: 238 GFVKK---KKIVEARQFFDSMNVR-DVVSWNTIITGYAQSGKIDEARQLFD--------E 297

Query: 367 PKLQH---YGCMVDILGKAGLVEGALKFIEEMPIEPNDIIWRTLLSACQNHENFTIGELI 426
             +Q    +  MV    +  +VE A +  ++MP E N++ W  +L+     E       +
Sbjct: 298 SPVQDVFTWTAMVSGYIQNRMVEEARELFDKMP-ERNEVSWNAMLAGYVQGERME----M 357

Query: 427 AKHLMTMDSC-NSSSYVLLSNIYARLGLWSAASKVRMMMKKQN 465
           AK L  +  C N S++  +   YA+ G  S A  +   M K++
Sbjct: 358 AKELFDVMPCRNVSTWNTMITGYAQCGKISEAKNLFDKMPKRD 373

BLAST of Cucsa.257610 vs. Swiss-Prot
Match: PPR21_ARATH (Pentatricopeptide repeat-containing protein At1g08070, chloroplastic OS=Arabidopsis thaliana GN=PCMP-H12 PE=2 SV=1)

HSP 1 Score: 354.4 bits (908), Expect = 2.1e-96
Identity = 192/506 (37.94%), Postives = 311/506 (61.46%), Query Frame = 1

Query: 10  PFLWNAVIKS--YSHGNEPVRALLEIGSNL--FLLNCLIAMYLRCGDIEFARQVFDRMPI 69
           PF+  +  KS  +  G +    +L++G +L  ++   LI+MY++ G +E A +VFD+ P 
Sbjct: 138 PFVLKSCAKSKAFKEGQQIHGHVLKLGCDLDLYVHTSLISMYVQNGRLEDAHKVFDKSPH 197

Query: 70  QDSVSYNSMIDGYVKSGTIDLARELFDSMPLEDKNLISWNSMLGGFAQTKDGIGLALELF 129
           +D VSY ++I GY   G I+ A++LFD +P++D  ++SWN+M+ G+A+T +    ALELF
Sbjct: 198 RDVVSYTALIKGYASRGYIENAQKLFDEIPVKD--VVSWNAMISGYAETGN-YKEALELF 257

Query: 130 EKMPER----DLVSWNTIIGGFAKCGRIEFAHSLFNRMPKRDVIS----WSNMIDGYAKL 189
           + M +     D  +  T++   A+ G IE    +   +      S     + +ID Y+K 
Sbjct: 258 KDMMKTNVRPDESTMVTVVSACAQSGSIELGRQVHLWIDDHGFGSNLKIVNALIDLYSKC 317

Query: 190 GDIKVARTLFDEMPDKDVVAFNTIMAGYAQNGYYTEALEIFHEMQRQSNLSPDETTLVVA 249
           G+++ A  LF+ +P KDV+++NT++ GY     Y EAL +F EM R S  +P++ T++  
Sbjct: 318 GELETACGLFERLPYKDVISWNTLIGGYTHMNLYKEALLLFQEMLR-SGETPNDVTMLSI 377

Query: 250 LSAISQLGHVEKAASMHNYFLEN--GISVTGKVAVALIDMYSKCGSIENAILIFDGVDQK 309
           L A + LG ++    +H Y  +   G++    +  +LIDMY+KCG IE A  +F+ +  K
Sbjct: 378 LPACAHLGAIDIGRWIHVYIDKRLKGVTNASSLRTSLIDMYAKCGDIEAAHQVFNSILHK 437

Query: 310 GIDHWNAMISGMARNGLGKLAFGMLLEMHRLSVKPDGITFIGVLNACAHAGLVKEGLICF 369
            +  WNAMI G A +G    +F +   M ++ ++PD ITF+G+L+AC+H+G++  G   F
Sbjct: 438 SLSSWNAMIFGFAMHGRADASFDLFSRMRKIGIQPDDITFVGLLSACSHSGMLDLGRHIF 497

Query: 370 ELMRKVHKLEPKLQHYGCMVDILGKAGLVEGALKFIEEMPIEPNDIIWRTLLSACQNHEN 429
             M + +K+ PKL+HYGCM+D+LG +GL + A + I  M +EP+ +IW +LL AC+ H N
Sbjct: 498 RTMTQDYKMTPKLEHYGCMIDLLGHSGLFKEAEEMINMMEMEPDGVIWCSLLKACKMHGN 557

Query: 430 FTIGELIAKHLMTMDSCNSSSYVLLSNIYARLGLWSAASKVRMMMKKQNLTKVPGCSWIE 489
             +GE  A++L+ ++  N  SYVLLSNIYA  G W+  +K R ++  + + KVPGCS IE
Sbjct: 558 VELGESFAENLIKIEPENPGSYVLLSNIYASAGRWNEVAKTRALLNDKGMKKVPGCSSIE 617

Query: 490 LEGVVHEFLVRDKSHPYVSEIYSVLD 502
           ++ VVHEF++ DK HP   EIY +L+
Sbjct: 618 IDSVVHEFIIGDKFHPRNREIYGMLE 639

HSP 2 Score: 124.0 bits (310), Expect = 4.7e-27
Identity = 104/383 (27.15%), Postives = 169/383 (44.13%), Query Frame = 1

Query: 12  LWNAVIKSYSHGNEPVRALLEIGSNLFLLNCLIAMYLRCGDIEFARQVFDRMPIQDSVSY 71
           +WN + + ++  ++PV AL        L  C+I++ L      F   V        +   
Sbjct: 101 IWNTMFRGHALSSDPVSALK-------LYVCMISLGLLPNSYTFPF-VLKSCAKSKAFKE 160

Query: 72  NSMIDGYVKSGTIDLARELFDSMPLEDKNLISWNSMLGGFAQTKDGIGLALELFEKMPER 131
              I G+V     DL             +L    S++  + Q    +  A ++F+K P R
Sbjct: 161 GQQIHGHVLKLGCDL-------------DLYVHTSLISMYVQN-GRLEDAHKVFDKSPHR 220

Query: 132 DLVSWNTIIGGFAKCGRIEFAHSLFNRMPKRDVISWSNMIDGYAKLGDIKVARTLFDEM- 191
           D+VS+  +I G+A  G IE A  LF+ +P +DV+SW+ MI GYA+ G+ K A  LF +M 
Sbjct: 221 DVVSYTALIKGYASRGYIENAQKLFDEIPVKDVVSWNAMISGYAETGNYKEALELFKDMM 280

Query: 192 -----PDKDVVAFNTIMAGYAQNGYYTEALEIFHEMQRQSNLSPDETTLVVALSAISQLG 251
                PD+  +   T+++  AQ+G          E+ RQ +L  D               
Sbjct: 281 KTNVRPDESTMV--TVVSACAQSGSI--------ELGRQVHLWID--------------- 340

Query: 252 HVEKAASMHNYFLENGISVTGKVAVALIDMYSKCGSIENAILIFDGVDQKGIDHWNAMIS 311
                        ++G     K+  ALID+YSKCG +E A  +F+ +  K +  WN +I 
Sbjct: 341 -------------DHGFGSNLKIVNALIDLYSKCGELETACGLFERLPYKDVISWNTLIG 400

Query: 312 GMARNGLGKLAFGMLLEMHRLSVKPDGITFIGVLNACAHAGLVKEG-LICFELMRKVHKL 371
           G     L K A  +  EM R    P+ +T + +L ACAH G +  G  I   + +++  +
Sbjct: 401 GYTHMNLYKEALLLFQEMLRSGETPNDVTMLSILPACAHLGAIDIGRWIHVYIDKRLKGV 423

Query: 372 EPKLQHYGCMVDILGKAGLVEGA 388
                    ++D+  K G +E A
Sbjct: 461 TNASSLRTSLIDMYAKCGDIEAA 423

BLAST of Cucsa.257610 vs. Swiss-Prot
Match: PP261_ARATH (Pentatricopeptide repeat-containing protein At3g29230 OS=Arabidopsis thaliana GN=PCMP-E27 PE=2 SV=1)

HSP 1 Score: 342.4 bits (877), Expect = 8.4e-93
Identity = 171/433 (39.49%), Postives = 270/433 (62.36%), Query Frame = 1

Query: 72  NSMIDGYVKSGTIDL--ARELFDSMPLEDKNLISWNSMLGGFAQTKDGIGLALELFEKMP 131
           N++ID Y + G + +  A +LF+ M   +++ +SWNSMLGG  +  + +  A  LF++MP
Sbjct: 156 NALIDCYSRCGGLGVRDAMKLFEKM--SERDTVSWNSMLGGLVKAGE-LRDARRLFDEMP 215

Query: 132 ERDLVSWNTIIGGFAKCGRIEFAHSLFNRMPKRDVISWSNMIDGYAKLGDIKVARTLFDE 191
           +RDL+SWNT++ G+A+C  +  A  LF +MP+R+ +SWS M+ GY+K GD+++AR +FD+
Sbjct: 216 QRDLISWNTMLDGYARCREMSKAFELFEKMPERNTVSWSTMVMGYSKAGDMEMARVMFDK 275

Query: 192 MP--DKDVVAFNTIMAGYAQNGYYTEALEIFHEMQRQSNLSPDETTLVVALSAISQLGHV 251
           MP   K+VV +  I+AGYA+ G   EA  +  +M   S L  D   ++  L+A ++ G +
Sbjct: 276 MPLPAKNVVTWTIIIAGYAEKGLLKEADRLVDQMVA-SGLKFDAAAVISILAACTESGLL 335

Query: 252 EKAASMHNYFLENGISVTGKVAVALIDMYSKCGSIENAILIFDGVDQKGIDHWNAMISGM 311
                +H+    + +     V  AL+DMY+KCG+++ A  +F+ + +K +  WN M+ G+
Sbjct: 336 SLGMRIHSILKRSNLGSNAYVLNALLDMYAKCGNLKKAFDVFNDIPKKDLVSWNTMLHGL 395

Query: 312 ARNGLGKLAFGMLLEMHRLSVKPDGITFIGVLNACAHAGLVKEGLICFELMRKVHKLEPK 371
             +G GK A  +   M R  ++PD +TFI VL +C HAGL+ EG+  F  M KV+ L P+
Sbjct: 396 GVHGHGKEAIELFSRMRREGIRPDKVTFIAVLCSCNHAGLIDEGIDYFYSMEKVYDLVPQ 455

Query: 372 LQHYGCMVDILGKAGLVEGALKFIEEMPIEPNDIIWRTLLSACQNHENFTIGELIAKHLM 431
           ++HYGC+VD+LG+ G ++ A+K ++ MP+EPN +IW  LL AC+ H    I + +  +L+
Sbjct: 456 VEHYGCLVDLLGRVGRLKEAIKVVQTMPMEPNVVIWGALLGACRMHNEVDIAKEVLDNLV 515

Query: 432 TMDSCNSSSYVLLSNIYARLGLWSAASKVRMMMKKQNLTKVPGCSWIELEGVVHEFLVRD 491
            +D C+  +Y LLSNIYA    W   + +R  MK   + K  G S +ELE  +HEF V D
Sbjct: 516 KLDPCDPGNYSLLSNIYAAAEDWEGVADIRSKMKSMGVEKPSGASSVELEDGIHEFTVFD 575

Query: 492 KSHPYVSEIYSVL 501
           KSHP   +IY +L
Sbjct: 576 KSHPKSDQIYQML 584

HSP 2 Score: 159.1 bits (401), Expect = 1.3e-37
Identity = 121/398 (30.40%), Postives = 206/398 (51.76%), Query Frame = 1

Query: 13  WNAVIKSYSHGNEPVRALLEIGSNLFLLNCLIAMYLRCGD--IEFARQVFDRMPIQDSVS 72
           W  V+K   +  E     L + S++++ N LI  Y RCG   +  A ++F++M  +D+VS
Sbjct: 132 WLPVVKMMHNHIEK----LGLSSDIYVPNALIDCYSRCGGLGVRDAMKLFEKMSERDTVS 191

Query: 73  YNSMIDGYVKSGTIDLARELFDSMPLEDKNLISWNSMLGGFAQTKDGIGLALELFEKMPE 132
           +NSM+ G VK+G +  AR LFD MP  D  LISWN+ML G+A+ ++ +  A ELFEKMPE
Sbjct: 192 WNSMLGGLVKAGELRDARRLFDEMPQRD--LISWNTMLDGYARCRE-MSKAFELFEKMPE 251

Query: 133 RDLVSWNTIIGGFAKCGRIEFAHSLFNRM--PKRDVISWSNMIDGYAKLGDIKVARTLFD 192
           R+ VSW+T++ G++K G +E A  +F++M  P ++V++W+ +I GYA+ G +K A  L D
Sbjct: 252 RNTVSWSTMVMGYSKAGDMEMARVMFDKMPLPAKNVVTWTIIIAGYAEKGLLKEADRLVD 311

Query: 193 EMPDK----DVVAFNTIMAGYAQNGYYTEALEIFHEMQRQSNLSPDETTLVVALSAISQL 252
           +M       D  A  +I+A   ++G  +  + I H + ++SNL  +   L   L   ++ 
Sbjct: 312 QMVASGLKFDAAAVISILAACTESGLLSLGMRI-HSILKRSNLGSNAYVLNALLDMYAKC 371

Query: 253 GHVEKAASMHNYFLEN------------GISVTGKVAVALIDMYSKCG------------ 312
           G+++KA  + N   +             G+   GK A+ L     + G            
Sbjct: 372 GNLKKAFDVFNDIPKKDLVSWNTMLHGLGVHGHGKEAIELFSRMRREGIRPDKVTFIAVL 431

Query: 313 -SIENAILIFDGVDQ-----------KGIDHWNAMISGMARNGLGKLAFGMLLEMHRLSV 367
            S  +A LI +G+D              ++H+  ++  + R G  K A  ++  M    +
Sbjct: 432 CSCNHAGLIDEGIDYFYSMEKVYDLVPQVEHYGCLVDLLGRVGRLKEAIKVVQTM---PM 491

HSP 3 Score: 78.2 bits (191), Expect = 3.0e-13
Identity = 77/329 (23.40%), Postives = 149/329 (45.29%), Query Frame = 1

Query: 145 KCGRIEFAHSLFNRMPKR----DVISWSNMIDGYAKLGDIKVARTLFDEMPDKDVVAFNT 204
           KC  +     L  ++ +R    D+     +I   +      +A  +F+++ + +V   N+
Sbjct: 28  KCANLNQVKQLHAQIIRRNLHEDLHIAPKLISALSLCRQTNLAVRVFNQVQEPNVHLCNS 87

Query: 205 IMAGYAQNGYYTEALEIFHEMQRQSNLSPDETTLVVALSAISQLGHVEKAASMHNYFLEN 264
           ++  +AQN    +A  +F EMQR   L  D  T    L A S    +     MHN+  + 
Sbjct: 88  LIRAHAQNSQPYQAFFVFSEMQR-FGLFADNFTYPFLLKACSGQSWLPVVKMMHNHIEKL 147

Query: 265 GISVTGKVAVALIDMYSKCG--SIENAILIFDGVDQKGIDHWNAMISGMARNGLGKLAFG 324
           G+S    V  ALID YS+CG   + +A+ +F+ + ++    WN+M+ G+ + G  + A  
Sbjct: 148 GLSSDIYVPNALIDCYSRCGGLGVRDAMKLFEKMSERDTVSWNSMLGGLVKAGELRDARR 207

Query: 325 MLLEMHRLSVKPDGITFIGVLNACAHAGLVKEGLICFELMRKVHKLEPKLQHYGCMVDIL 384
           +  EM     + D I++  +L+  A     +E    FEL  K+   E     +  MV   
Sbjct: 208 LFDEM----PQRDLISWNTMLDGYARC---REMSKAFELFEKMP--ERNTVSWSTMVMGY 267

Query: 385 GKAGLVEGALKFIEEMPIEPNDIIWRTLLSACQNHENF--TIGELIAKHLMTMDSCNSSS 444
            KAG +E A    ++MP+   +++  T++ A    +        L+ + + +    ++++
Sbjct: 268 SKAGDMEMARVMFDKMPLPAKNVVTWTIIIAGYAEKGLLKEADRLVDQMVASGLKFDAAA 327

Query: 445 YVLLSNIYARLGLWSAASKVRMMMKKQNL 466
            + +       GL S   ++  ++K+ NL
Sbjct: 328 VISILAACTESGLLSLGMRIHSILKRSNL 346

HSP 4 Score: 41.6 bits (96), Expect = 3.1e-02
Identity = 70/357 (19.61%), Postives = 148/357 (41.46%), Query Frame = 1

Query: 120 LALELFEKMPERDLVSWNTIIGGFAKCGRIEFAHSLFNRMPKRDVISWSNMIDGYAKLGD 179
           L  ++  +    DL     +I   + C +   A  +FN++ + +V   +++I  +A+   
Sbjct: 38  LHAQIIRRNLHEDLHIAPKLISALSLCRQTNLAVRVFNQVQEPNVHLCNSLIRAHAQNSQ 97

Query: 180 IKVARTLFDEMPDKDVVAFN---TIMAGYAQNGYYTEALEIFHEMQRQSNLSPD---ETT 239
              A  +F EM    + A N     +        +   +++ H    +  LS D      
Sbjct: 98  PYQAFFVFSEMQRFGLFADNFTYPFLLKACSGQSWLPVVKMMHNHIEKLGLSSDIYVPNA 157

Query: 240 LVVALSAISQLGHVEKAASMHNYFLENGISVTGKVAVALIDMYSKCGSIENAILIFDGVD 299
           L+   S    LG V  A  +     E        +   L+    K G + +A  +FD + 
Sbjct: 158 LIDCYSRCGGLG-VRDAMKLFEKMSERDTVSWNSMLGGLV----KAGELRDARRLFDEMP 217

Query: 300 QKGIDHWNAMISGMARNGLGKLAFGMLLEMHRLSVKPDGITFIGVLNACAHAGLVKEGLI 359
           Q+ +  WN M+ G AR      AF +  +M   +     +++  ++   + AG ++   +
Sbjct: 218 QRDLISWNTMLDGYARCREMSKAFELFEKMPERNT----VSWSTMVMGYSKAGDMEMARV 277

Query: 360 CFELMRKVHKLEPKLQHYGCMVDILGKAGLVEGALKFIEEM---PIEPNDIIWRTLLSAC 419
            F+ M    K    +  +  ++    + GL++ A + +++M    ++ +     ++L+AC
Sbjct: 278 MFDKMPLPAK---NVVTWTIIIAGYAEKGLLKEADRLVDQMVASGLKFDAAAVISILAAC 337

Query: 420 QNHENFTIGELIAKHLMTMDSCNSSSYVL--LSNIYARLGLWSAASKVRMMMKKQNL 466
                 ++G  I   ++   +  S++YVL  L ++YA+ G    A  V   + K++L
Sbjct: 338 TESGLLSLGMRI-HSILKRSNLGSNAYVLNALLDMYAKCGNLKKAFDVFNDIPKKDL 381

BLAST of Cucsa.257610 vs. TrEMBL
Match: A0A0A0L4E1_CUCSA (Uncharacterized protein OS=Cucumis sativus GN=Csa_3G126180 PE=4 SV=1)

HSP 1 Score: 1020.8 bits (2638), Expect = 5.9e-295
Identity = 515/558 (92.29%), Postives = 515/558 (92.29%), Query Frame = 1

Query: 1   IRRNHLDDDPFLWNAVIKSYSHGNEPVRAL------------------------------ 60
           IRRNHLDDDPFLWNAVIKSYSHGNEPVRAL                              
Sbjct: 81  IRRNHLDDDPFLWNAVIKSYSHGNEPVRALVLFCMMLENGFCVDKFSFSLILKACARVCL 140

Query: 61  -------------LEIGSNLFLLNCLIAMYLRCGDIEFARQVFDRMPIQDSVSYNSMIDG 120
                        LEIGSNLFLLNCLIAMYLRCGDIEFARQVFDRMPIQDSVSYNSMIDG
Sbjct: 141 VEEGKQIHGLLMKLEIGSNLFLLNCLIAMYLRCGDIEFARQVFDRMPIQDSVSYNSMIDG 200

Query: 121 YVKSGTIDLARELFDSMPLEDKNLISWNSMLGGFAQTKDGIGLALELFEKMPERDLVSWN 180
           YVKSGTIDLARELFDSMPLEDKNLISWNSMLGGFAQTKDGIGLALELFEKMPERDLVSWN
Sbjct: 201 YVKSGTIDLARELFDSMPLEDKNLISWNSMLGGFAQTKDGIGLALELFEKMPERDLVSWN 260

Query: 181 TIIGGFAKCGRIEFAHSLFNRMPKRDVISWSNMIDGYAKLGDIKVARTLFDEMPDKDVVA 240
           TIIGGFAKCGRIEFAHSLFNRMPKRDVISWSNMIDGYAKLGDIKVARTLFDEMPDKDVVA
Sbjct: 261 TIIGGFAKCGRIEFAHSLFNRMPKRDVISWSNMIDGYAKLGDIKVARTLFDEMPDKDVVA 320

Query: 241 FNTIMAGYAQNGYYTEALEIFHEMQRQSNLSPDETTLVVALSAISQLGHVEKAASMHNYF 300
           FNTIMAGYAQNGYYTEALEIFHEMQRQSNLSPDETTLVVALSAISQLGHVEKAASMHNYF
Sbjct: 321 FNTIMAGYAQNGYYTEALEIFHEMQRQSNLSPDETTLVVALSAISQLGHVEKAASMHNYF 380

Query: 301 LENGISVTGKVAVALIDMYSKCGSIENAILIFDGVDQKGIDHWNAMISGMARNGLGKLAF 360
           LENGISVTGKVAVALIDMYSKCGSIENAILIFDGVDQKGIDHWNAMISGMARNGLGKLAF
Sbjct: 381 LENGISVTGKVAVALIDMYSKCGSIENAILIFDGVDQKGIDHWNAMISGMARNGLGKLAF 440

Query: 361 GMLLEMHRLSVKPDGITFIGVLNACAHAGLVKEGLICFELMRKVHKLEPKLQHYGCMVDI 420
           GMLLEMHRLSVKPDGITFIGVLNACAHAGLVKEGLICFELMRKVHKLEPKLQHYGCMVDI
Sbjct: 441 GMLLEMHRLSVKPDGITFIGVLNACAHAGLVKEGLICFELMRKVHKLEPKLQHYGCMVDI 500

Query: 421 LGKAGLVEGALKFIEEMPIEPNDIIWRTLLSACQNHENFTIGELIAKHLMTMDSCNSSSY 480
           LGKAGLVEGALKFIEEMPIEPNDIIWRTLLSACQNHENFTIGELIAKHLMTMDSCNSSSY
Sbjct: 501 LGKAGLVEGALKFIEEMPIEPNDIIWRTLLSACQNHENFTIGELIAKHLMTMDSCNSSSY 560

Query: 481 VLLSNIYARLGLWSAASKVRMMMKKQNLTKVPGCSWIELEGVVHEFLVRDKSHPYVSEIY 516
           VLLSNIYARLGLWSAASKVRMMMKKQNLTKVPGCSWIELEGVVHEFLVRDKSHPYVSEIY
Sbjct: 561 VLLSNIYARLGLWSAASKVRMMMKKQNLTKVPGCSWIELEGVVHEFLVRDKSHPYVSEIY 620

BLAST of Cucsa.257610 vs. TrEMBL
Match: A0A0D2SGV5_GOSRA (Uncharacterized protein OS=Gossypium raimondii GN=B456_007G090700 PE=4 SV=1)

HSP 1 Score: 697.2 bits (1798), Expect = 1.5e-197
Identity = 331/547 (60.51%), Postives = 420/547 (76.78%), Query Frame = 1

Query: 7   DDDPFLWNAVIKSYSHGNEPVRAL------------------------------------ 66
           D+DPFLWNAVIKSYSHG E   A                                     
Sbjct: 84  DEDPFLWNAVIKSYSHGREAQEAFIILCLMLENGVPFDKFSLSLVLKACSQLGLLKEGMQ 143

Query: 67  -------LEIGSNLFLLNCLIAMYLRCGDIEFARQVFDRMPIQDSVSYNSMIDGYVKSGT 126
                  L  GS+LFL NCLI+ YLRCG I +ARQ+FDRM ++DSVSYNSMIDGY+K G 
Sbjct: 144 VHGLLRKLNFGSDLFLQNCLISYYLRCGFIGYARQLFDRMSMRDSVSYNSMIDGYIKRGM 203

Query: 127 IDLARELFDSMPLEDKNLISWNSMLGGFAQTKDGIGLALELFEKMPERDLVSWNTIIGGF 186
           IDLA+ELFD MPLE KNLI+WNSM+ G+ Q KDG+GLAL LFEKMP+RDL+SWN++I G+
Sbjct: 204 IDLAKELFDVMPLEKKNLITWNSMISGYTQLKDGMGLALGLFEKMPDRDLISWNSMINGY 263

Query: 187 AKCGRIEFAHSLFNRMPKRDVISWSNMIDGYAKLGDIKVARTLFDEMPDKDVVAFNTIMA 246
            KCG +E A  LF++MP+ DV+SW+NMI+GYAK+G I +AR+LF+EM ++D+V  N +MA
Sbjct: 264 VKCGNMEDAQILFDKMPRWDVVSWANMINGYAKVGKIDLARSLFNEMSERDIVVCNAMMA 323

Query: 247 GYAQNGYYTEALEIFHEMQRQSNLSPDETTLVVALSAISQLGHVEKAASMHNYFLENGIS 306
           G  QNGY  EALEIF+ +QR  +L PD  TL++ALSAI+QLGHV+K  ++H+Y  +   S
Sbjct: 324 GCVQNGYCGEALEIFYHLQRNCSLQPDSATLLIALSAIAQLGHVDKGLALHHYLKQKKFS 383

Query: 307 VTGKVAVALIDMYSKCGSIENAILIFDGVDQKGIDHWNAMISGMARNGLGKLAFGMLLEM 366
           + G++ VALIDMYSKCGSI++A+L+F+G+  K +DHWNAMI G+A +G+G+LAF +L++M
Sbjct: 384 LGGRLGVALIDMYSKCGSIKDAVLVFEGIKYKTVDHWNAMIGGLAIHGMGELAFKLLMDM 443

Query: 367 HRLSVKPDGITFIGVLNACAHAGLVKEGLICFELMRKVHKLEPKLQHYGCMVDILGKAGL 426
            +L V+PD IT+IGV+NAC HAGLVKEGL+CF++MR+VHK+ PKLQHYGCMVDILG+AG 
Sbjct: 444 EKLCVEPDDITYIGVMNACGHAGLVKEGLVCFDIMRRVHKMVPKLQHYGCMVDILGRAGQ 503

Query: 427 VEGALKFIEEMPIEPNDIIWRTLLSACQNHENFTIGELIAKHLMTMDSCNSSSYVLLSNI 486
           ++ A KFIEEMPIE ND+IWR+LLSAC+NHEN  +GE  AKHL+T+DSCNSSSYVLLSN+
Sbjct: 504 IDVARKFIEEMPIEANDVIWRSLLSACKNHENIDVGEPAAKHLITLDSCNSSSYVLLSNM 563

Query: 487 YARLGLWSAASKVRMMMKKQNLTKVPGCSWIELEGVVHEFLVRDKSHPYVSEIYSVLDGF 511
           YA LG+W+A S VR+MMK++NL K+PGCSWIELEG VHEF V+DKSHP V EIYS+LD  
Sbjct: 564 YAGLGMWNAVSSVRLMMKERNLKKIPGCSWIELEGTVHEFFVQDKSHPQVMEIYSLLDSL 623

BLAST of Cucsa.257610 vs. TrEMBL
Match: A0A061E1W2_THECC (Pentatricopeptide repeat (PPR) superfamily protein, putative OS=Theobroma cacao GN=TCM_005523 PE=4 SV=1)

HSP 1 Score: 693.7 bits (1789), Expect = 1.7e-196
Identity = 327/548 (59.67%), Postives = 419/548 (76.46%), Query Frame = 1

Query: 9   DPFLWNAVIKSYSHGNEPVRAL-------------------------------------- 68
           DPFLWNA IKSYSHG +  +A                                       
Sbjct: 86  DPFLWNAAIKSYSHGRDAKQAFVMLCLMLENGVRFDTFSLSLVLKACSRLGLMKEGMQVH 145

Query: 69  -----LEIGSNLFLLNCLIAMYLRCGDIEFARQVFDRMPIQDSVSYNSMIDGYVKSGTID 128
                L  GS+LFL NCLI++Y+RCG + +ARQ+FDRM  +DSVSYNSMIDGY+K+G I 
Sbjct: 146 GLLRKLNFGSDLFLQNCLISLYIRCGFVGYARQLFDRMCRRDSVSYNSMIDGYIKTGMIH 205

Query: 129 LARELFDSMPLEDKNLISWNSMLGGFAQTKDGIGLALELFEKMPERDLVSWNTIIGGFAK 188
           LARELF+ MP+EDKNLI+WNSM+ G+AQ + G+ +AL+LFE+MP+RDL+SWN++I GF K
Sbjct: 206 LARELFEFMPMEDKNLITWNSMISGYAQLEYGMVMALKLFEEMPDRDLISWNSMINGFVK 265

Query: 189 CGRIEFAHSLFNRMPKRDVISWSNMIDGYAKLGDIKVARTLFDEMPDKDVVAFNTIMAGY 248
           CG +E A +LF +MPKRDV+SW+NMI+GYAK+G I +AR LFDEMP++DVV  N +M GY
Sbjct: 266 CGNMEDAQALFVKMPKRDVVSWANMINGYAKVGKIDLARRLFDEMPERDVVVCNAMMGGY 325

Query: 249 AQNGYYTEALEIFHEMQRQSNLSPDETTLVVALSAISQLGHVEKAASMHNYFLENGISVT 308
            QNGY +EALEIF+ +QR  NL PD  TL++ LSAI+QLG ++K  ++H Y  EN  S++
Sbjct: 326 VQNGYCSEALEIFYHLQRYDNLQPDNATLLIVLSAIAQLGRIDKGLAIHQYLEENKFSLS 385

Query: 309 GKVAVALIDMYSKCGSIENAILIFDGVDQKGIDHWNAMISGMARNGLGKLAFGMLLEMHR 368
           G++ VALIDMYSKCGS++N +L+F+ ++ + +DHWNAMI G+A +GLG+LAF +L+EM R
Sbjct: 386 GRLGVALIDMYSKCGSVKNGMLVFESIEDRSVDHWNAMIGGLAIHGLGELAFSLLIEMER 445

Query: 369 LSVKPDGITFIGVLNACAHAGLVKEGLICFELMRKVHKLEPKLQHYGCMVDILGKAGLVE 428
           LSV+PD IT+IGVLNAC HAGLVKEGLICF++MR+VHK+ PKL+HYGC+VDILG+AG + 
Sbjct: 446 LSVEPDDITYIGVLNACGHAGLVKEGLICFDIMRRVHKMVPKLKHYGCIVDILGRAGQIA 505

Query: 429 GALKFIEEMPIEPNDIIWRTLLSACQNHENFTIGELIAKHLMTMDSCNSSSYVLLSNIYA 488
            A KFIEEMPIE ND+IWR+LLSAC+NHEN  +GE +A+HL+++DSCNSSSY+LLSN+YA
Sbjct: 506 EARKFIEEMPIEANDVIWRSLLSACKNHENIDVGEPVAEHLISLDSCNSSSYILLSNMYA 565

Query: 489 RLGLWSAASKVRMMMKKQNLTKVPGCSWIELEGVVHEFLVRDKSHPYVSEIYSVLDGFGA 514
            LG+WSA S VRM MK++NL K+PGCSWIELEG VHEF V+DKSHP V EIYS+L  F  
Sbjct: 566 GLGMWSAVSNVRMRMKERNLKKIPGCSWIELEGTVHEFFVQDKSHPQVMEIYSLLGSFSI 625

BLAST of Cucsa.257610 vs. TrEMBL
Match: F6I380_VITVI (Putative uncharacterized protein OS=Vitis vinifera GN=VIT_15s0048g00690 PE=4 SV=1)

HSP 1 Score: 692.6 bits (1786), Expect = 3.7e-196
Identity = 329/556 (59.17%), Postives = 423/556 (76.08%), Query Frame = 1

Query: 2   RRNHLDDDPFLWNAVIKSYSHGNEPVRAL------------------------------- 61
           R++   D+PFLWNA+IKS+SHG +P  A                                
Sbjct: 79  RKHRKQDNPFLWNAIIKSFSHGEDPREAFVIFNLMLENGVCVDKFSFSLVLKACSRLGLI 138

Query: 62  ------------LEIGSNLFLLNCLIAMYLRCGDIEFARQVFDRMPIQDSVSYNSMIDGY 121
                       +EIGS++FL NCL+ +YLRCG +  ARQ+FDRM  +DSVS+NSMIDGY
Sbjct: 139 KEGMQIHGLLGRMEIGSDVFLQNCLMCLYLRCGCLGIARQLFDRMMKRDSVSFNSMIDGY 198

Query: 122 VKSGTIDLARELFDSMPLEDKNLISWNSMLGGFAQTKDGIGLALELFEKMPERDLVSWNT 181
           VK G +  ARELFD MP+E KNLISWNSM+ G+A++++G+ +A ELFE+MP+RDL+SWN+
Sbjct: 199 VKHGMVKSARELFDVMPMEQKNLISWNSMISGYARSEEGLRVAWELFEEMPKRDLISWNS 258

Query: 182 IIGGFAKCGRIEFAHSLFNRMPKRDVISWSNMIDGYAKLGDIKVARTLFDEMPDKDVVAF 241
           +I G  KCG++E AH LFN+MPKRDV+SW+NM+DGYAKLG+I +AR LFDEMP++DV++ 
Sbjct: 259 MIDGCVKCGKMENAHHLFNQMPKRDVVSWANMVDGYAKLGEIDIARGLFDEMPERDVISC 318

Query: 242 NTIMAGYAQNGYYTEALEIFHEMQRQSNLSPDETTLVVALSAISQLGHVEKAASMHNYFL 301
           N +MAGY QNG+  EAL  FH+M  +  L PD  TL++ LSAI+QLGH ++  ++H Y  
Sbjct: 319 NAMMAGYVQNGHLMEALNFFHDMLSRKELFPDNATLLITLSAIAQLGHFDEGVALHCYIE 378

Query: 302 ENGISVTGKVAVALIDMYSKCGSIENAILIFDGVDQKGIDHWNAMISGMARNGLGKLAFG 361
           +NG S++ K+ VALIDMY+KCGSI+NA+ +F+ +D K IDHWNA+I G+A +GLG++AF 
Sbjct: 379 DNGFSLSEKLGVALIDMYAKCGSIDNALSVFEDIDDKSIDHWNAIIGGLAIHGLGEVAFE 438

Query: 362 MLLEMHRLSVKPDGITFIGVLNACAHAGLVKEGLICFELMRKVHKLEPKLQHYGCMVDIL 421
           + +EM +L VKPD ITFIGVLNAC HAGLVKEGL+CFELMR+VHK+EPKLQHYGCMVDIL
Sbjct: 439 LFMEMEKLFVKPDDITFIGVLNACNHAGLVKEGLMCFELMRRVHKVEPKLQHYGCMVDIL 498

Query: 422 GKAGLVEGALKFIEEMPIEPNDIIWRTLLSACQNHENFTIGELIAKHLMTMDSCNSSSYV 481
           G+AG VE A KF+E+MPIEPND++WRTLLSAC+NHENFTIGE +AKHL+++DS N SSYV
Sbjct: 499 GRAGHVEEAKKFVEKMPIEPNDVVWRTLLSACRNHENFTIGEPVAKHLISVDSYNPSSYV 558

Query: 482 LLSNIYARLGLWSAASKVRMMMKKQNLTKVPGCSWIELEGVVHEFLVRDKSHPYVSEIYS 515
           LLSNIYA  G+W+   ++RMMMK+++L K+PGCS IELEG VHEF VRDKSHP V EIYS
Sbjct: 559 LLSNIYAGFGMWNDVYRIRMMMKQRDLKKIPGCSQIELEGNVHEFFVRDKSHPQVREIYS 618

BLAST of Cucsa.257610 vs. TrEMBL
Match: V5QP58_GOSHI (Pentatricopeptide repeat-containing protein OS=Gossypium hirsutum PE=2 SV=1)

HSP 1 Score: 691.8 bits (1784), Expect = 6.3e-196
Identity = 331/547 (60.51%), Postives = 419/547 (76.60%), Query Frame = 1

Query: 7   DDDPFLWNAVIKSYSHGNEPVRAL------------------------------------ 66
           D+DPFLWNAVIKSYSHG E   A                                     
Sbjct: 84  DEDPFLWNAVIKSYSHGREAQEAFIILCLMLENGVPFDKFSLSLVLKACSQLGLLKEGMQ 143

Query: 67  -------LEIGSNLFLLNCLIAMYLRCGDIEFARQVFDRMPIQDSVSYNSMIDGYVKSGT 126
                  L  GS+LFL NCLI+ YLRCG I +ARQ+FDRM ++DSVSYNSMIDGY+K G 
Sbjct: 144 VHGLLRKLNFGSDLFLQNCLISYYLRCGFIGYARQLFDRMSMRDSVSYNSMIDGYIKRGM 203

Query: 127 IDLARELFDSMPLEDKNLISWNSMLGGFAQTKDGIGLALELFEKMPERDLVSWNTIIGGF 186
           IDLA+ELFD MPLE+KNLI+WNSM+ G+ Q KDG+GLAL LFEKMP+RDL+SWN++I GF
Sbjct: 204 IDLAKELFDVMPLENKNLITWNSMISGYTQLKDGMGLALGLFEKMPDRDLISWNSMINGF 263

Query: 187 AKCGRIEFAHSLFNRMPKRDVISWSNMIDGYAKLGDIKVARTLFDEMPDKDVVAFNTIMA 246
            KCG +E A  LF++MP+RDV+SW+NMI+GYAK+G I +AR+LF+EM ++D+V  N +MA
Sbjct: 264 VKCGNMEDAQILFDKMPRRDVVSWANMINGYAKVGKIDLARSLFNEMSERDIVVCNAMMA 323

Query: 247 GYAQNGYYTEALEIFHEMQRQSNLSPDETTLVVALSAISQLGHVEKAASMHNYFLENGIS 306
           G  QNGY  EALEIF+ + R  +L PD  TL++ALSAI+QLGHV++  ++H+Y  +   S
Sbjct: 324 GCVQNGYCGEALEIFYHLPRNCSLQPDSATLLIALSAIAQLGHVDRGLALHHYLKQKKFS 383

Query: 307 VTGKVAVALIDMYSKCGSIENAILIFDGVDQKGIDHWNAMISGMARNGLGKLAFGMLLEM 366
           + G++ VALIDMYSKCGSI+NA+L+F+G+  K +DHWNAMI G+A +G+G+LAF +L++M
Sbjct: 384 LGGRLGVALIDMYSKCGSIKNAVLVFEGIKYKTVDHWNAMIGGLAIHGMGELAFKLLMDM 443

Query: 367 HRLSVKPDGITFIGVLNACAHAGLVKEGLICFELMRKVHKLEPKLQHYGCMVDILGKAGL 426
            +L V+PD IT+IGVLNAC HAGLVKEGLICF++MR+VHK+ PKLQHYGC+VDILG+AG 
Sbjct: 444 EKLCVEPDDITYIGVLNACGHAGLVKEGLICFDIMRRVHKMVPKLQHYGCVVDILGRAGQ 503

Query: 427 VEGALKFIEEMPIEPNDIIWRTLLSACQNHENFTIGELIAKHLMTMDSCNSSSYVLLSNI 486
           ++ A KFIEEMPIE ND+IWR+LLSAC+NHEN  +GE   KHL+T+DSCNSSSYVLLSN+
Sbjct: 504 IDVARKFIEEMPIEANDVIWRSLLSACKNHENIEVGEPAGKHLITLDSCNSSSYVLLSNM 563

Query: 487 YARLGLWSAASKVRMMMKKQNLTKVPGCSWIELEGVVHEFLVRDKSHPYVSEIYSVLDGF 511
           YA LG+W+A S VR+MMK++NL K+PGCS IELEG VHEF V+DKSHP V EIYS+LD  
Sbjct: 564 YAGLGMWNAVSSVRLMMKERNLKKIPGCSRIELEGTVHEFFVQDKSHPQVMEIYSLLDSL 623

BLAST of Cucsa.257610 vs. TAIR10
Match: AT2G45350.1 (AT2G45350.1 Pentatricopeptide repeat (PPR) superfamily protein)

HSP 1 Score: 628.6 bits (1620), Expect = 3.3e-180
Identity = 302/521 (57.97%), Postives = 388/521 (74.47%), Query Frame = 1

Query: 8   DDPFLWNAVIKSYSHGNEPVRALL------------------------------------ 67
           +DPFLWNAVIKS+SHG +P +ALL                                    
Sbjct: 84  EDPFLWNAVIKSHSHGKDPRQALLLLCLMLENGVSVDKFSLSLVLKACSRLGFVKGGMQI 143

Query: 68  -------EIGSNLFLLNCLIAMYLRCGDIEFARQVFDRMPIQDSVSYNSMIDGYVKSGTI 127
                   + S+LFL NCLI +YL+CG +  +RQ+FDRMP +DSVSYNSMIDGYVK G I
Sbjct: 144 HGFLKKTGLWSDLFLQNCLIGLYLKCGCLGLSRQMFDRMPKRDSVSYNSMIDGYVKCGLI 203

Query: 128 DLARELFDSMPLEDKNLISWNSMLGGFAQTKDGIGLALELFEKMPERDLVSWNTIIGGFA 187
             ARELFD MP+E KNLISWNSM+ G+AQT DG+ +A +LF  MPE+DL+SWN++I G+ 
Sbjct: 204 VSARELFDLMPMEMKNLISWNSMISGYAQTSDGVDIASKLFADMPEKDLISWNSMIDGYV 263

Query: 188 KCGRIEFAHSLFNRMPKRDVISWSNMIDGYAKLGDIKVARTLFDEMPDKDVVAFNTIMAG 247
           K GRIE A  LF+ MP+RDV++W+ MIDGYAKLG +  A+TLFD+MP +DVVA+N++MAG
Sbjct: 264 KHGRIEDAKGLFDVMPRRDVVTWATMIDGYAKLGFVHHAKTLFDQMPHRDVVAYNSMMAG 323

Query: 248 YAQNGYYTEALEIFHEMQRQSNLSPDETTLVVALSAISQLGHVEKAASMHNYFLENGISV 307
           Y QN Y+ EALEIF +M+++S+L PD+TTLV+ L AI+QLG + KA  MH Y +E    +
Sbjct: 324 YVQNKYHMEALEIFSDMEKESHLLPDDTTLVIVLPAIAQLGRLSKAIDMHLYIVEKQFYL 383

Query: 308 TGKVAVALIDMYSKCGSIENAILIFDGVDQKGIDHWNAMISGMARNGLGKLAFGMLLEMH 367
            GK+ VALIDMYSKCGSI++A+L+F+G++ K IDHWNAMI G+A +GLG+ AF MLL++ 
Sbjct: 384 GGKLGVALIDMYSKCGSIQHAMLVFEGIENKSIDHWNAMIGGLAIHGLGESAFDMLLQIE 443

Query: 368 RLSVKPDGITFIGVLNACAHAGLVKEGLICFELMRKVHKLEPKLQHYGCMVDILGKAGLV 427
           RLS+KPD ITF+GVLNAC+H+GLVKEGL+CFELMR+ HK+EP+LQHYGCMVDIL ++G +
Sbjct: 444 RLSLKPDDITFVGVLNACSHSGLVKEGLLCFELMRRKHKIEPRLQHYGCMVDILSRSGSI 503

Query: 428 EGALKFIEEMPIEPNDIIWRTLLSACQNHENFTIGELIAKHLMTMDSCNSSSYVLLSNIY 486
           E A   IEEMP+EPND+IWRT L+AC +H+ F  GEL+AKHL+     N SSYVLLSN+Y
Sbjct: 504 ELAKNLIEEMPVEPNDVIWRTFLTACSHHKEFETGELVAKHLILQAGYNPSSYVLLSNMY 563

BLAST of Cucsa.257610 vs. TAIR10
Match: AT2G29760.1 (AT2G29760.1 Tetratricopeptide repeat (TPR)-like superfamily protein)

HSP 1 Score: 371.7 bits (953), Expect = 7.3e-103
Identity = 190/473 (40.17%), Postives = 291/473 (61.52%), Query Frame = 1

Query: 33  IGSNLFLLNCLIAMYLRCGDIEFARQVFDRMPIQDSVSYNSMIDGYVKSGTIDLARELFD 92
           +GS++F+ N LI  Y  CGD++ A +VF  +  +D VS+NSMI+G+V+ G+ D A ELF 
Sbjct: 162 VGSDVFVANSLIHCYFSCGDLDSACKVFTTIKEKDVVSWNSMINGFVQKGSPDKALELFK 221

Query: 93  SMPLEDKNL--ISWNSMLGGFAQTKD---GIGLALELFEKMPERDLVSWNTIIGGFAKCG 152
            M  ED     ++   +L   A+ ++   G  +   + E     +L   N ++  + KCG
Sbjct: 222 KMESEDVKASHVTMVGVLSACAKIRNLEFGRQVCSYIEENRVNVNLTLANAMLDMYTKCG 281

Query: 153 RIEFAHSLFNRMPKRDVISWSNMIDGYAKLGDIKVARTLFDEMPDKDVVAFNTIMAGYAQ 212
            IE A  LF+ M ++D ++W+ M+DGYA   D + AR + + MP KD+VA+N +++ Y Q
Sbjct: 282 SIEDAKRLFDAMEEKDNVTWTTMLDGYAISEDYEAAREVLNSMPQKDIVAWNALISAYEQ 341

Query: 213 NGYYTEALEIFHEMQRQSNLSPDETTLVVALSAISQLGHVEKAASMHNYFLENGISVTGK 272
           NG   EAL +FHE+Q Q N+  ++ TLV  LSA +Q+G +E    +H+Y  ++GI +   
Sbjct: 342 NGKPNEALIVFHELQLQKNMKLNQITLVSTLSACAQVGALELGRWIHSYIKKHGIRMNFH 401

Query: 273 VAVALIDMYSKCGSIENAILIFDGVDQKGIDHWNAMISGMARNGLGKLAFGMLLEMHRLS 332
           V  ALI MYSKCG +E +  +F+ V+++ +  W+AMI G+A +G G  A  M  +M   +
Sbjct: 402 VTSALIHMYSKCGDLEKSREVFNSVEKRDVFVWSAMIGGLAMHGCGNEAVDMFYKMQEAN 461

Query: 333 VKPDGITFIGVLNACAHAGLVKEGLICFELMRKVHKLEPKLQHYGCMVDILGKAGLVEGA 392
           VKP+G+TF  V  AC+H GLV E    F  M   + + P+ +HY C+VD+LG++G +E A
Sbjct: 462 VKPNGVTFTNVFCACSHTGLVDEAESLFHQMESNYGIVPEEKHYACIVDVLGRSGYLEKA 521

Query: 393 LKFIEEMPIEPNDIIWRTLLSACQNHENFTIGELIAKHLMTMDSCNSSSYVLLSNIYARL 452
           +KFIE MPI P+  +W  LL AC+ H N  + E+    L+ ++  N  ++VLLSNIYA+L
Sbjct: 522 VKFIEAMPIPPSTSVWGALLGACKIHANLNLAEMACTRLLELEPRNDGAHVLLSNIYAKL 581

Query: 453 GLWSAASKVRMMMKKQNLTKVPGCSWIELEGVVHEFLVRDKSHPYVSEIYSVL 501
           G W   S++R  M+   L K PGCS IE++G++HEFL  D +HP   ++Y  L
Sbjct: 582 GKWENVSELRKHMRVTGLKKEPGCSSIEIDGMIHEFLSGDNAHPMSEKVYGKL 634

HSP 2 Score: 108.6 bits (270), Expect = 1.2e-23
Identity = 76/281 (27.05%), Postives = 144/281 (51.25%), Query Frame = 1

Query: 83  TIDLARELFDSMPLEDKNLISWNSMLGGFAQTKDGIGLALELFEKMPERDLV----SWNT 142
           +++ AR++FD +P    N  +WN+++  +A   D +       + + E        ++  
Sbjct: 79  SLEYARKVFDEIP--KPNSFAWNTLIRAYASGPDPVLSIWAFLDMVSESQCYPNKYTFPF 138

Query: 143 IIGGFAKCGRIEFAHSLFNRMPKR----DVISWSNMIDGYAKLGDIKVARTLFDEMPDKD 202
           +I   A+   +    SL     K     DV   +++I  Y   GD+  A  +F  + +KD
Sbjct: 139 LIKAAAEVSSLSLGQSLHGMAVKSAVGSDVFVANSLIHCYFSCGDLDSACKVFTTIKEKD 198

Query: 203 VVAFNTIMAGYAQNGYYTEALEIFHEMQRQSNLSPDETTLVVALSAISQLGHVEKAASMH 262
           VV++N+++ G+ Q G   +ALE+F +M+ + ++     T+V  LSA +++ ++E    + 
Sbjct: 199 VVSWNSMINGFVQKGSPDKALELFKKMESE-DVKASHVTMVGVLSACAKIRNLEFGRQVC 258

Query: 263 NYFLENGISVTGKVAVALIDMYSKCGSIENAILIFDGVDQKGIDHWNAMISGMARNGLGK 322
           +Y  EN ++V   +A A++DMY+KCGSIE+A  +FD +++K    W  M+ G A +   +
Sbjct: 259 SYIEENRVNVNLTLANAMLDMYTKCGSIEDAKRLFDAMEEKDNVTWTTMLDGYAISEDYE 318

Query: 323 LAFGMLLEMHRLSVKPDGITFIGVLNACAHAGLVKEGLICF 356
            A  +L  M     + D + +  +++A    G   E LI F
Sbjct: 319 AAREVLNSM----PQKDIVAWNALISAYEQNGKPNEALIVF 352

HSP 3 Score: 102.4 bits (254), Expect = 8.3e-22
Identity = 61/243 (25.10%), Postives = 121/243 (49.79%), Query Frame = 1

Query: 175 AKLGDIKVARTLFDEMPDKDVVAFNTIMAGYAQNGYYTEALEIFHEMQRQSNLSPDETTL 234
           +    ++ AR +FDE+P  +  A+NT++  YA       ++  F +M  +S   P++ T 
Sbjct: 75  SSFASLEYARKVFDEIPKPNSFAWNTLIRAYASGPDPVLSIWAFLDMVSESQCYPNKYTF 134

Query: 235 VVALSAISQLGHVEKAASMHNYFLENGISVTGKVAVALIDMYSKCGSIENAILIFDGVDQ 294
              + A +++  +    S+H   +++ +     VA +LI  Y  CG +++A  +F  + +
Sbjct: 135 PFLIKAAAEVSSLSLGQSLHGMAVKSAVGSDVFVANSLIHCYFSCGDLDSACKVFTTIKE 194

Query: 295 KGIDHWNAMISGMARNGLGKLAFGMLLEMHRLSVKPDGITFIGVLNACAHAGLVKEG-LI 354
           K +  WN+MI+G  + G    A  +  +M    VK   +T +GVL+ACA    ++ G  +
Sbjct: 195 KDVVSWNSMINGFVQKGSPDKALELFKKMESEDVKASHVTMVGVLSACAKIRNLEFGRQV 254

Query: 355 CFELMRKVHKLEPKLQHYGCMVDILGKAGLVEGALKFIEEMPIEPNDIIWRTLLSACQNH 414
           C  +  + +++   L     M+D+  K G +E A +  + M  E +++ W T+L      
Sbjct: 255 CSYI--EENRVNVNLTLANAMLDMYTKCGSIEDAKRLFDAME-EKDNVTWTTMLDGYAIS 314

Query: 415 ENF 417
           E++
Sbjct: 315 EDY 314

HSP 4 Score: 99.0 bits (245), Expect = 9.2e-21
Identity = 91/390 (23.33%), Postives = 177/390 (45.38%), Query Frame = 1

Query: 35  SNLFLLNCLIAMYLRCGDIEFARQVFDRMPIQDSVSYNSMIDGYVKSGTIDLARELFDSM 94
           S LF +  L +       +E+AR+VFD +P  +S ++N++I  Y       L+   F  M
Sbjct: 66  SKLFAMAALSSF----ASLEYARKVFDEIPKPNSFAWNTLIRAYASGPDPVLSIWAFLDM 125

Query: 95  PLEDK---NLISWNSMLGGFAQTKD-GIGLALE--LFEKMPERDLVSWNTIIGGFAKCGR 154
             E +   N  ++  ++   A+     +G +L     +     D+   N++I  +  CG 
Sbjct: 126 VSESQCYPNKYTFPFLIKAAAEVSSLSLGQSLHGMAVKSAVGSDVFVANSLIHCYFSCGD 185

Query: 155 IEFAHSLFNRMPKRDVISWSNMIDGYAKLGDIKVARTLFDEMPDKDVVAFNTIMAGYAQN 214
           ++ A  +F  + ++DV+SW++MI+G+ + G    A  LF +M  +DV A +  M G    
Sbjct: 186 LDSACKVFTTIKEKDVVSWNSMINGFVQKGSPDKALELFKKMESEDVKASHVTMVGVLSA 245

Query: 215 GYYTEALEIFHEM---QRQSNLSPDETTLVVALSAISQLGHVEKAASMHNYFLENGISVT 274
                 LE   ++     ++ ++ + T     L   ++ G +E A  + +  +E   +VT
Sbjct: 246 CAKIRNLEFGRQVCSYIEENRVNVNLTLANAMLDMYTKCGSIEDAKRLFD-AMEEKDNVT 305

Query: 275 GKVAVALIDMYSKCGSIENAILIFDGVDQKGIDHWNAMISGMARNGLGKLAFGMLLEMH- 334
                 ++D Y+     E A  + + + QK I  WNA+IS   +NG    A  +  E+  
Sbjct: 306 W---TTMLDGYAISEDYEAAREVLNSMPQKDIVAWNALISAYEQNGKPNEALIVFHELQL 365

Query: 335 RLSVKPDGITFIGVLNACAHAGLVKEGLICFELMRKVHKLEPKLQHYGCMVDILGKAGLV 394
           + ++K + IT +  L+ACA  G ++ G      ++K H +         ++ +  K G +
Sbjct: 366 QKNMKLNQITLVSTLSACAQVGALELGRWIHSYIKK-HGIRMNFHVTSALIHMYSKCGDL 425

Query: 395 EGALKFIEEMPIEPNDI-IWRTLLSACQNH 414
           E + +      +E  D+ +W  ++     H
Sbjct: 426 EKSREVFNS--VEKRDVFVWSAMIGGLAMH 444

BLAST of Cucsa.257610 vs. TAIR10
Match: AT4G02750.1 (AT4G02750.1 Tetratricopeptide repeat (TPR)-like superfamily protein)

HSP 1 Score: 369.4 bits (947), Expect = 3.6e-102
Identity = 198/493 (40.16%), Postives = 297/493 (60.24%), Query Frame = 1

Query: 13  WNAVIKSYSHGNEPVRALLEIGSN----LFLLNCLIAMYLRCGDIEFARQVFDRMPIQDS 72
           WNA++ +Y   ++   A +   S     L   NCL+  +++   I  ARQ FD M ++D 
Sbjct: 191 WNALLSAYVQNSKMEEACMLFKSRENWALVSWNCLLGGFVKKKKIVEARQFFDSMNVRDV 250

Query: 73  VSYNSMIDGYVKSGTIDLARELFDSMPLEDKNLISWNSMLGGFAQTKDGIGLALELFEKM 132
           VS+N++I GY +SG ID AR+LFD  P++D  + +W +M+ G+ Q +  +  A ELF+KM
Sbjct: 251 VSWNTIITGYAQSGKIDEARQLFDESPVQD--VFTWTAMVSGYIQNRM-VEEARELFDKM 310

Query: 133 PERDLVSWNTIIGGFAKCGRIEFAHSLFNRMPKRDVISWSNMIDGYAKLGDIKVARTLFD 192
           PER+ VSWN ++ G+ +  R+E A  LF+ MP R+V +W+ MI GYA+ G I  A+ LFD
Sbjct: 311 PERNEVSWNAMLAGYVQGERMEMAKELFDVMPCRNVSTWNTMITGYAQCGKISEAKNLFD 370

Query: 193 EMPDKDVVAFNTIMAGYAQNGYYTEALEIFHEMQRQSNLSPDETTLVVALSAISQLGHVE 252
           +MP +D V++  ++AGY+Q+G+  EAL +F +M+R+     + ++   ALS  + +  +E
Sbjct: 371 KMPKRDPVSWAAMIAGYSQSGHSFEALRLFVQMEREGGRL-NRSSFSSALSTCADVVALE 430

Query: 253 KAASMHNYFLENGISVTGKVAVALIDMYSKCGSIENAILIFDGVDQKGIDHWNAMISGMA 312
               +H   ++ G      V  AL+ MY KCGSIE A  +F  +  K I  WN MI+G +
Sbjct: 431 LGKQLHGRLVKGGYETGCFVGNALLLMYCKCGSIEEANDLFKEMAGKDIVSWNTMIAGYS 490

Query: 313 RNGLGKLAFGMLLEMHRLSVKPDGITFIGVLNACAHAGLVKEGLICFELMRKVHKLEPKL 372
           R+G G++A      M R  +KPD  T + VL+AC+H GLV +G   F  M + + + P  
Sbjct: 491 RHGFGEVALRFFESMKREGLKPDDATMVAVLSACSHTGLVDKGRQYFYTMTQDYGVMPNS 550

Query: 373 QHYGCMVDILGKAGLVEGALKFIEEMPIEPNDIIWRTLLSACQNHENFTIGELIAKHLMT 432
           QHY CMVD+LG+AGL+E A   ++ MP EP+  IW TLL A + H N  + E  A  +  
Sbjct: 551 QHYACMVDLLGRAGLLEDAHNLMKNMPFEPDAAIWGTLLGASRVHGNTELAETAADKIFA 610

Query: 433 MDSCNSSSYVLLSNIYARLGLWSAASKVRMMMKKQNLTKVPGCSWIELEGVVHEFLVRDK 492
           M+  NS  YVLLSN+YA  G W    K+R+ M+ + + KVPG SWIE++   H F V D+
Sbjct: 611 MEPENSGMYVLLSNLYASSGRWGDVGKLRVRMRDKGVKKVPGYSWIEIQNKTHTFSVGDE 670

Query: 493 SHPYVSEIYSVLD 502
            HP   EI++ L+
Sbjct: 671 FHPEKDEIFAFLE 679

HSP 2 Score: 177.6 bits (449), Expect = 2.0e-44
Identity = 120/380 (31.58%), Postives = 197/380 (51.84%), Query Frame = 1

Query: 41  NCLIAMYLRCGDIEFARQVFDRMPIQDSVSYNSMIDGYVKSGTIDLARELFDSMPLEDKN 100
           N +I  Y+R  ++  AR++F+ MP +D  S+N+M+ GY ++G +D AR +FD MP  +KN
Sbjct: 130 NVMIKGYVRNRNLGKARELFEIMPERDVCSWNTMLSGYAQNGCVDDARSVFDRMP--EKN 189

Query: 101 LISWNSMLGGFAQTKDGIGLALELFEKMPERDLVSWNTIIGGFAKCGRIEFAHSLFNRMP 160
            +SWN++L  + Q    +  A  LF+      LVSWN ++GGF K  +I  A   F+ M 
Sbjct: 190 DVSWNALLSAYVQNSK-MEEACMLFKSRENWALVSWNCLLGGFVKKKKIVEARQFFDSMN 249

Query: 161 KRDVISWSNMIDGYAKLGDIKVARTLFDEMPDKDVVAFNTIMAGYAQNGYYTEALEIFHE 220
            RDV+SW+ +I GYA+ G I  AR LFDE P +DV  +  +++GY QN    EA E+F +
Sbjct: 250 VRDVVSWNTIITGYAQSGKIDEARQLFDESPVQDVFTWTAMVSGYIQNRMVEEARELFDK 309

Query: 221 MQRQSNLSPDETTLVVALSAISQLGHVEKAASMHNYFLENGISVTGKVAVALIDMYSKCG 280
           M  ++ +S +       L+   Q   +E A  + +      +S        +I  Y++CG
Sbjct: 310 MPERNEVSWN-----AMLAGYVQGERMEMAKELFDVMPCRNVSTWN----TMITGYAQCG 369

Query: 281 SIENAILIFDGVDQKGIDHWNAMISGMARNGLGKLAFGMLLEMHRLSVKPDGITFIGVLN 340
            I  A  +FD + ++    W AMI+G +++G    A  + ++M R   + +  +F   L+
Sbjct: 370 KISEAKNLFDKMPKRDPVSWAAMIAGYSQSGHSFEALRLFVQMEREGGRLNRSSFSSALS 429

Query: 341 ACAHAGLVKEGLICFELMRKVH-KLEPKLQHYGCMVD-----ILGKAGLVEGALKFIEEM 400
            CA        ++  EL +++H +L       GC V      +  K G +E A    +EM
Sbjct: 430 TCA-------DVVALELGKQLHGRLVKGGYETGCFVGNALLLMYCKCGSIEEANDLFKEM 488

Query: 401 PIEPNDII-WRTLLSACQNH 414
                DI+ W T+++    H
Sbjct: 490 --AGKDIVSWNTMIAGYSRH 488

HSP 3 Score: 165.6 bits (418), Expect = 8.0e-41
Identity = 118/359 (32.87%), Postives = 188/359 (52.37%), Query Frame = 1

Query: 7   DDDPFLWNAVIKSYSHG---NEPVRALLEIGS-NLFLLNCLIAMYLRCGDIEFARQVFDR 66
           D D   WN  I SY      NE +R    +   +    N +I+ YLR G+ E AR++FD 
Sbjct: 61  DSDIKEWNVAISSYMRTGRCNEALRVFKRMPRWSSVSYNGMISGYLRNGEFELARKLFDE 120

Query: 67  MPIQDSVSYNSMIDGYVKSGTIDLARELFDSMPLEDKNLISWNSMLGGFAQTKDGIGLAL 126
           MP +D VS+N MI GYV++  +  ARELF+ MP  ++++ SWN+ML G+AQ    +  A 
Sbjct: 121 MPERDLVSWNVMIKGYVRNRNLGKARELFEIMP--ERDVCSWNTMLSGYAQN-GCVDDAR 180

Query: 127 ELFEKMPERDLVSWNTIIGGFAKCGRIEFAHSLFNRMPKRDVISWSNMIDGYAKLGDIKV 186
            +F++MPE++ VSWN ++  + +  ++E A  LF       ++SW+ ++ G+ K   I  
Sbjct: 181 SVFDRMPEKNDVSWNALLSAYVQNSKMEEACMLFKSRENWALVSWNCLLGGFVKKKKIVE 240

Query: 187 ARTLFDEMPDKDVVAFNTIMAGYAQNGYYTEALEIFHEMQRQSNLSPDETTLVVALSAIS 246
           AR  FD M  +DVV++NTI+ GYAQ+G   EA ++F E   Q     D  T    +S   
Sbjct: 241 ARQFFDSMNVRDVVSWNTIITGYAQSGKIDEARQLFDESPVQ-----DVFTWTAMVSGYI 300

Query: 247 QLGHVEKAASMHNYFLE-NGISVTGKVAVALIDMYSKCGSIENAILIFDGVDQKGIDHWN 306
           Q   VE+A  + +   E N +S       A++  Y +   +E A  +FD +  + +  WN
Sbjct: 301 QNRMVEEARELFDKMPERNEVSWN-----AMLAGYVQGERMEMAKELFDVMPCRNVSTWN 360

Query: 307 AMISGMARNGLGKLAFGMLLEMHRLSVKPDGITFIGVLNACAHAGLVKEGLICFELMRK 361
            MI+G A+ G    A  +  +M     K D +++  ++   + +G   E L  F  M +
Sbjct: 361 TMITGYAQCGKISEAKNLFDKM----PKRDPVSWAAMIAGYSQSGHSFEALRLFVQMER 402

HSP 4 Score: 162.9 bits (411), Expect = 5.2e-40
Identity = 134/462 (29.00%), Postives = 226/462 (48.92%), Query Frame = 1

Query: 13  WNAVIKSYSHGNE--PVRALLEI--GSNLFLLNCLIAMYLRCGDIEFARQVFDRMPIQDS 72
           WN +IK Y         R L EI    ++   N +++ Y + G ++ AR VFDRMP ++ 
Sbjct: 129 WNVMIKGYVRNRNLGKARELFEIMPERDVCSWNTMLSGYAQNGCVDDARSVFDRMPEKND 188

Query: 73  VSYNSMIDGYVKSGTIDLARELFDSMPLEDKNLISWNSMLGGFAQTKDGIGLALELFEKM 132
           VS+N+++  YV++  ++ A  LF S   E+  L+SWN +LGGF + K  I  A + F+ M
Sbjct: 189 VSWNALLSAYVQNSKMEEACMLFKSR--ENWALVSWNCLLGGFVKKKK-IVEARQFFDSM 248

Query: 133 PERDLVSWNTIIGGFAKCGRIEFAHSLFNRMPKRDVISWSNMIDGYAKLGDIKVARTLFD 192
             RD+VSWNTII G+A+ G+I+ A  LF+  P +DV +W+ M+ GY +   ++ AR LFD
Sbjct: 249 NVRDVVSWNTIITGYAQSGKIDEARQLFDESPVQDVFTWTAMVSGYIQNRMVEEARELFD 308

Query: 193 EMPDKDVVAFNTIMAGYAQNGYYTEALEIFHEMQRQSNLSPDETTLVVALSAISQLGHVE 252
           +MP+++ V++N ++AGY Q      A E+F  M  +     + +T    ++  +Q G + 
Sbjct: 309 KMPERNEVSWNAMLAGYVQGERMEMAKELFDVMPCR-----NVSTWNTMITGYAQCGKIS 368

Query: 253 KAASMHNYFLENGISVTGKVAVALIDMYSKCGSIENAILIFDGVDQKGIDHWNAMISGMA 312
           +A ++ +   +           A+I  YS+ G    A+ +F  ++++G     +  S   
Sbjct: 369 EAKNLFDKMPKR----DPVSWAAMIAGYSQSGHSFEALRLFVQMEREGGRLNRSSFSSAL 428

Query: 313 RNGLGKLAFGMLLEMHRLSVKPDGIT--FIG--VLNACAHAGLVKEGLICFELMRKVHKL 372
                 +A  +  ++H   VK    T  F+G  +L      G ++E    F+ M      
Sbjct: 429 STCADVVALELGKQLHGRLVKGGYETGCFVGNALLLMYCKCGSIEEANDLFKEM-----A 488

Query: 373 EPKLQHYGCMVDILGKAGLVEGALKFIEEMP---IEPNDIIWRTLLSACQNHENFTIGEL 432
              +  +  M+    + G  E AL+F E M    ++P+D     +LSAC +H        
Sbjct: 489 GKDIVSWNTMIAGYSRHGFGEVALRFFESMKREGLKPDDATMVAVLSAC-SHTGLVDKGR 548

Query: 433 IAKHLMTMD---SCNSSSYVLLSNIYARLGLWSAASKVRMMM 461
              + MT D     NS  Y  + ++  R GL   A  +   M
Sbjct: 549 QYFYTMTQDYGVMPNSQHYACMVDLLGRAGLLEDAHNLMKNM 572

HSP 5 Score: 118.6 bits (296), Expect = 1.1e-26
Identity = 87/311 (27.97%), Postives = 157/311 (50.48%), Query Frame = 1

Query: 98  DKNLISWNSMLGGFAQTKDGIGLALELFEKMPERDLVSWNTIIGGFAKCGRIEFAHSLFN 157
           D ++  WN  +  + +T      AL +F++MP    VS+N +I G+ + G  E A  LF+
Sbjct: 61  DSDIKEWNVAISSYMRT-GRCNEALRVFKRMPRWSSVSYNGMISGYLRNGEFELARKLFD 120

Query: 158 RMPKRDVISWSNMIDGYAKLGDIKVARTLFDEMPDKDVVAFNTIMAGYAQNGYYTEALEI 217
            MP+RD++SW+ MI GY +  ++  AR LF+ MP++DV ++NT+++GYAQNG   +A  +
Sbjct: 121 EMPERDLVSWNVMIKGYVRNRNLGKARELFEIMPERDVCSWNTMLSGYAQNGCVDDARSV 180

Query: 218 FHEMQRQSNLSPDETTLVVALSAISQLGHVEKAASMHNYFLENGISVTGKVAVALIDMYS 277
           F  M  ++++S +       LSA  Q   +E+A  +     EN   V+      L+  + 
Sbjct: 181 FDRMPEKNDVSWN-----ALLSAYVQNSKMEEACMLFK-SRENWALVSWN---CLLGGFV 240

Query: 278 KCGSIENAILIFDGVDQKGIDHWNAMISGMARNGLGKLAFGMLLEMHRLSVKPDGITFIG 337
           K   I  A   FD ++ + +  WN +I+G A++G    A  +  E    S   D  T+  
Sbjct: 241 KKKKIVEARQFFDSMNVRDVVSWNTIITGYAQSGKIDEARQLFDE----SPVQDVFTWTA 300

Query: 338 VLNACAHAGLVKEGLICFELMRKVHKLEPKLQHYGCMVDILGKAGLVEGALKFIEEMPIE 397
           +++      +V+E    F+ M + +++      +  M+    +   +E A +  + MP  
Sbjct: 301 MVSGYIQNRMVEEARELFDKMPERNEVS-----WNAMLAGYVQGERMEMAKELFDVMPCR 351

Query: 398 PNDIIWRTLLS 409
            N   W T+++
Sbjct: 361 -NVSTWNTMIT 351

HSP 6 Score: 107.8 bits (268), Expect = 2.0e-23
Identity = 90/343 (26.24%), Postives = 161/343 (46.94%), Query Frame = 1

Query: 127 KMPERDLVSWNTIIGGFAKCGRIEFAHSLFNRMPKRDVISWSNMIDGYAKLGDIKVARTL 186
           K  + D+  WN  I  + + GR   A  +F RMP+   +S++ MI GY + G+ ++AR L
Sbjct: 58  KCGDSDIKEWNVAISSYMRTGRCNEALRVFKRMPRWSSVSYNGMISGYLRNGEFELARKL 117

Query: 187 FDEMPDKDVVAFNTIMAGYAQNGYYTEALEIFHEMQRQSNLSPDETTLVVALSAISQLGH 246
           FDEMP++D+V++N ++ GY +N    +A E+F  M  +     D  +    LS  +Q G 
Sbjct: 118 FDEMPERDLVSWNVMIKGYVRNRNLGKARELFEIMPER-----DVCSWNTMLSGYAQNGC 177

Query: 247 VEKAASMHNYFLE-NGISVTGKVAVALIDMYSKCGSIENAILIFDGVDQKGIDHWNAMIS 306
           V+ A S+ +   E N +S       AL+  Y +   +E A ++F   +   +  WN ++ 
Sbjct: 178 VDDARSVFDRMPEKNDVSWN-----ALLSAYVQNSKMEEACMLFKSRENWALVSWNCLLG 237

Query: 307 GMARNGLGKLAFGMLLEMHRLSVKPDGITFIGVLNACAHAGLVKEGLICFELMRKVHKLE 366
           G  +    K           ++V+ D +++  ++   A +G + E    F+        E
Sbjct: 238 GFVKK---KKIVEARQFFDSMNVR-DVVSWNTIITGYAQSGKIDEARQLFD--------E 297

Query: 367 PKLQH---YGCMVDILGKAGLVEGALKFIEEMPIEPNDIIWRTLLSACQNHENFTIGELI 426
             +Q    +  MV    +  +VE A +  ++MP E N++ W  +L+     E       +
Sbjct: 298 SPVQDVFTWTAMVSGYIQNRMVEEARELFDKMP-ERNEVSWNAMLAGYVQGERME----M 357

Query: 427 AKHLMTMDSC-NSSSYVLLSNIYARLGLWSAASKVRMMMKKQN 465
           AK L  +  C N S++  +   YA+ G  S A  +   M K++
Sbjct: 358 AKELFDVMPCRNVSTWNTMITGYAQCGKISEAKNLFDKMPKRD 373

BLAST of Cucsa.257610 vs. TAIR10
Match: AT1G08070.1 (AT1G08070.1 Tetratricopeptide repeat (TPR)-like superfamily protein)

HSP 1 Score: 354.4 bits (908), Expect = 1.2e-97
Identity = 192/506 (37.94%), Postives = 311/506 (61.46%), Query Frame = 1

Query: 10  PFLWNAVIKS--YSHGNEPVRALLEIGSNL--FLLNCLIAMYLRCGDIEFARQVFDRMPI 69
           PF+  +  KS  +  G +    +L++G +L  ++   LI+MY++ G +E A +VFD+ P 
Sbjct: 138 PFVLKSCAKSKAFKEGQQIHGHVLKLGCDLDLYVHTSLISMYVQNGRLEDAHKVFDKSPH 197

Query: 70  QDSVSYNSMIDGYVKSGTIDLARELFDSMPLEDKNLISWNSMLGGFAQTKDGIGLALELF 129
           +D VSY ++I GY   G I+ A++LFD +P++D  ++SWN+M+ G+A+T +    ALELF
Sbjct: 198 RDVVSYTALIKGYASRGYIENAQKLFDEIPVKD--VVSWNAMISGYAETGN-YKEALELF 257

Query: 130 EKMPER----DLVSWNTIIGGFAKCGRIEFAHSLFNRMPKRDVIS----WSNMIDGYAKL 189
           + M +     D  +  T++   A+ G IE    +   +      S     + +ID Y+K 
Sbjct: 258 KDMMKTNVRPDESTMVTVVSACAQSGSIELGRQVHLWIDDHGFGSNLKIVNALIDLYSKC 317

Query: 190 GDIKVARTLFDEMPDKDVVAFNTIMAGYAQNGYYTEALEIFHEMQRQSNLSPDETTLVVA 249
           G+++ A  LF+ +P KDV+++NT++ GY     Y EAL +F EM R S  +P++ T++  
Sbjct: 318 GELETACGLFERLPYKDVISWNTLIGGYTHMNLYKEALLLFQEMLR-SGETPNDVTMLSI 377

Query: 250 LSAISQLGHVEKAASMHNYFLEN--GISVTGKVAVALIDMYSKCGSIENAILIFDGVDQK 309
           L A + LG ++    +H Y  +   G++    +  +LIDMY+KCG IE A  +F+ +  K
Sbjct: 378 LPACAHLGAIDIGRWIHVYIDKRLKGVTNASSLRTSLIDMYAKCGDIEAAHQVFNSILHK 437

Query: 310 GIDHWNAMISGMARNGLGKLAFGMLLEMHRLSVKPDGITFIGVLNACAHAGLVKEGLICF 369
            +  WNAMI G A +G    +F +   M ++ ++PD ITF+G+L+AC+H+G++  G   F
Sbjct: 438 SLSSWNAMIFGFAMHGRADASFDLFSRMRKIGIQPDDITFVGLLSACSHSGMLDLGRHIF 497

Query: 370 ELMRKVHKLEPKLQHYGCMVDILGKAGLVEGALKFIEEMPIEPNDIIWRTLLSACQNHEN 429
             M + +K+ PKL+HYGCM+D+LG +GL + A + I  M +EP+ +IW +LL AC+ H N
Sbjct: 498 RTMTQDYKMTPKLEHYGCMIDLLGHSGLFKEAEEMINMMEMEPDGVIWCSLLKACKMHGN 557

Query: 430 FTIGELIAKHLMTMDSCNSSSYVLLSNIYARLGLWSAASKVRMMMKKQNLTKVPGCSWIE 489
             +GE  A++L+ ++  N  SYVLLSNIYA  G W+  +K R ++  + + KVPGCS IE
Sbjct: 558 VELGESFAENLIKIEPENPGSYVLLSNIYASAGRWNEVAKTRALLNDKGMKKVPGCSSIE 617

Query: 490 LEGVVHEFLVRDKSHPYVSEIYSVLD 502
           ++ VVHEF++ DK HP   EIY +L+
Sbjct: 618 IDSVVHEFIIGDKFHPRNREIYGMLE 639

HSP 2 Score: 124.0 bits (310), Expect = 2.7e-28
Identity = 104/383 (27.15%), Postives = 169/383 (44.13%), Query Frame = 1

Query: 12  LWNAVIKSYSHGNEPVRALLEIGSNLFLLNCLIAMYLRCGDIEFARQVFDRMPIQDSVSY 71
           +WN + + ++  ++PV AL        L  C+I++ L      F   V        +   
Sbjct: 101 IWNTMFRGHALSSDPVSALK-------LYVCMISLGLLPNSYTFPF-VLKSCAKSKAFKE 160

Query: 72  NSMIDGYVKSGTIDLARELFDSMPLEDKNLISWNSMLGGFAQTKDGIGLALELFEKMPER 131
              I G+V     DL             +L    S++  + Q    +  A ++F+K P R
Sbjct: 161 GQQIHGHVLKLGCDL-------------DLYVHTSLISMYVQN-GRLEDAHKVFDKSPHR 220

Query: 132 DLVSWNTIIGGFAKCGRIEFAHSLFNRMPKRDVISWSNMIDGYAKLGDIKVARTLFDEM- 191
           D+VS+  +I G+A  G IE A  LF+ +P +DV+SW+ MI GYA+ G+ K A  LF +M 
Sbjct: 221 DVVSYTALIKGYASRGYIENAQKLFDEIPVKDVVSWNAMISGYAETGNYKEALELFKDMM 280

Query: 192 -----PDKDVVAFNTIMAGYAQNGYYTEALEIFHEMQRQSNLSPDETTLVVALSAISQLG 251
                PD+  +   T+++  AQ+G          E+ RQ +L  D               
Sbjct: 281 KTNVRPDESTMV--TVVSACAQSGSI--------ELGRQVHLWID--------------- 340

Query: 252 HVEKAASMHNYFLENGISVTGKVAVALIDMYSKCGSIENAILIFDGVDQKGIDHWNAMIS 311
                        ++G     K+  ALID+YSKCG +E A  +F+ +  K +  WN +I 
Sbjct: 341 -------------DHGFGSNLKIVNALIDLYSKCGELETACGLFERLPYKDVISWNTLIG 400

Query: 312 GMARNGLGKLAFGMLLEMHRLSVKPDGITFIGVLNACAHAGLVKEG-LICFELMRKVHKL 371
           G     L K A  +  EM R    P+ +T + +L ACAH G +  G  I   + +++  +
Sbjct: 401 GYTHMNLYKEALLLFQEMLRSGETPNDVTMLSILPACAHLGAIDIGRWIHVYIDKRLKGV 423

Query: 372 EPKLQHYGCMVDILGKAGLVEGA 388
                    ++D+  K G +E A
Sbjct: 461 TNASSLRTSLIDMYAKCGDIEAA 423

BLAST of Cucsa.257610 vs. TAIR10
Match: AT3G29230.1 (AT3G29230.1 Tetratricopeptide repeat (TPR)-like superfamily protein)

HSP 1 Score: 342.4 bits (877), Expect = 4.8e-94
Identity = 171/433 (39.49%), Postives = 270/433 (62.36%), Query Frame = 1

Query: 72  NSMIDGYVKSGTIDL--ARELFDSMPLEDKNLISWNSMLGGFAQTKDGIGLALELFEKMP 131
           N++ID Y + G + +  A +LF+ M   +++ +SWNSMLGG  +  + +  A  LF++MP
Sbjct: 156 NALIDCYSRCGGLGVRDAMKLFEKM--SERDTVSWNSMLGGLVKAGE-LRDARRLFDEMP 215

Query: 132 ERDLVSWNTIIGGFAKCGRIEFAHSLFNRMPKRDVISWSNMIDGYAKLGDIKVARTLFDE 191
           +RDL+SWNT++ G+A+C  +  A  LF +MP+R+ +SWS M+ GY+K GD+++AR +FD+
Sbjct: 216 QRDLISWNTMLDGYARCREMSKAFELFEKMPERNTVSWSTMVMGYSKAGDMEMARVMFDK 275

Query: 192 MP--DKDVVAFNTIMAGYAQNGYYTEALEIFHEMQRQSNLSPDETTLVVALSAISQLGHV 251
           MP   K+VV +  I+AGYA+ G   EA  +  +M   S L  D   ++  L+A ++ G +
Sbjct: 276 MPLPAKNVVTWTIIIAGYAEKGLLKEADRLVDQMVA-SGLKFDAAAVISILAACTESGLL 335

Query: 252 EKAASMHNYFLENGISVTGKVAVALIDMYSKCGSIENAILIFDGVDQKGIDHWNAMISGM 311
                +H+    + +     V  AL+DMY+KCG+++ A  +F+ + +K +  WN M+ G+
Sbjct: 336 SLGMRIHSILKRSNLGSNAYVLNALLDMYAKCGNLKKAFDVFNDIPKKDLVSWNTMLHGL 395

Query: 312 ARNGLGKLAFGMLLEMHRLSVKPDGITFIGVLNACAHAGLVKEGLICFELMRKVHKLEPK 371
             +G GK A  +   M R  ++PD +TFI VL +C HAGL+ EG+  F  M KV+ L P+
Sbjct: 396 GVHGHGKEAIELFSRMRREGIRPDKVTFIAVLCSCNHAGLIDEGIDYFYSMEKVYDLVPQ 455

Query: 372 LQHYGCMVDILGKAGLVEGALKFIEEMPIEPNDIIWRTLLSACQNHENFTIGELIAKHLM 431
           ++HYGC+VD+LG+ G ++ A+K ++ MP+EPN +IW  LL AC+ H    I + +  +L+
Sbjct: 456 VEHYGCLVDLLGRVGRLKEAIKVVQTMPMEPNVVIWGALLGACRMHNEVDIAKEVLDNLV 515

Query: 432 TMDSCNSSSYVLLSNIYARLGLWSAASKVRMMMKKQNLTKVPGCSWIELEGVVHEFLVRD 491
            +D C+  +Y LLSNIYA    W   + +R  MK   + K  G S +ELE  +HEF V D
Sbjct: 516 KLDPCDPGNYSLLSNIYAAAEDWEGVADIRSKMKSMGVEKPSGASSVELEDGIHEFTVFD 575

Query: 492 KSHPYVSEIYSVL 501
           KSHP   +IY +L
Sbjct: 576 KSHPKSDQIYQML 584

HSP 2 Score: 159.1 bits (401), Expect = 7.5e-39
Identity = 121/398 (30.40%), Postives = 206/398 (51.76%), Query Frame = 1

Query: 13  WNAVIKSYSHGNEPVRALLEIGSNLFLLNCLIAMYLRCGD--IEFARQVFDRMPIQDSVS 72
           W  V+K   +  E     L + S++++ N LI  Y RCG   +  A ++F++M  +D+VS
Sbjct: 132 WLPVVKMMHNHIEK----LGLSSDIYVPNALIDCYSRCGGLGVRDAMKLFEKMSERDTVS 191

Query: 73  YNSMIDGYVKSGTIDLARELFDSMPLEDKNLISWNSMLGGFAQTKDGIGLALELFEKMPE 132
           +NSM+ G VK+G +  AR LFD MP  D  LISWN+ML G+A+ ++ +  A ELFEKMPE
Sbjct: 192 WNSMLGGLVKAGELRDARRLFDEMPQRD--LISWNTMLDGYARCRE-MSKAFELFEKMPE 251

Query: 133 RDLVSWNTIIGGFAKCGRIEFAHSLFNRM--PKRDVISWSNMIDGYAKLGDIKVARTLFD 192
           R+ VSW+T++ G++K G +E A  +F++M  P ++V++W+ +I GYA+ G +K A  L D
Sbjct: 252 RNTVSWSTMVMGYSKAGDMEMARVMFDKMPLPAKNVVTWTIIIAGYAEKGLLKEADRLVD 311

Query: 193 EMPDK----DVVAFNTIMAGYAQNGYYTEALEIFHEMQRQSNLSPDETTLVVALSAISQL 252
           +M       D  A  +I+A   ++G  +  + I H + ++SNL  +   L   L   ++ 
Sbjct: 312 QMVASGLKFDAAAVISILAACTESGLLSLGMRI-HSILKRSNLGSNAYVLNALLDMYAKC 371

Query: 253 GHVEKAASMHNYFLEN------------GISVTGKVAVALIDMYSKCG------------ 312
           G+++KA  + N   +             G+   GK A+ L     + G            
Sbjct: 372 GNLKKAFDVFNDIPKKDLVSWNTMLHGLGVHGHGKEAIELFSRMRREGIRPDKVTFIAVL 431

Query: 313 -SIENAILIFDGVDQ-----------KGIDHWNAMISGMARNGLGKLAFGMLLEMHRLSV 367
            S  +A LI +G+D              ++H+  ++  + R G  K A  ++  M    +
Sbjct: 432 CSCNHAGLIDEGIDYFYSMEKVYDLVPQVEHYGCLVDLLGRVGRLKEAIKVVQTM---PM 491

HSP 3 Score: 78.2 bits (191), Expect = 1.7e-14
Identity = 77/329 (23.40%), Postives = 149/329 (45.29%), Query Frame = 1

Query: 145 KCGRIEFAHSLFNRMPKR----DVISWSNMIDGYAKLGDIKVARTLFDEMPDKDVVAFNT 204
           KC  +     L  ++ +R    D+     +I   +      +A  +F+++ + +V   N+
Sbjct: 28  KCANLNQVKQLHAQIIRRNLHEDLHIAPKLISALSLCRQTNLAVRVFNQVQEPNVHLCNS 87

Query: 205 IMAGYAQNGYYTEALEIFHEMQRQSNLSPDETTLVVALSAISQLGHVEKAASMHNYFLEN 264
           ++  +AQN    +A  +F EMQR   L  D  T    L A S    +     MHN+  + 
Sbjct: 88  LIRAHAQNSQPYQAFFVFSEMQR-FGLFADNFTYPFLLKACSGQSWLPVVKMMHNHIEKL 147

Query: 265 GISVTGKVAVALIDMYSKCG--SIENAILIFDGVDQKGIDHWNAMISGMARNGLGKLAFG 324
           G+S    V  ALID YS+CG   + +A+ +F+ + ++    WN+M+ G+ + G  + A  
Sbjct: 148 GLSSDIYVPNALIDCYSRCGGLGVRDAMKLFEKMSERDTVSWNSMLGGLVKAGELRDARR 207

Query: 325 MLLEMHRLSVKPDGITFIGVLNACAHAGLVKEGLICFELMRKVHKLEPKLQHYGCMVDIL 384
           +  EM     + D I++  +L+  A     +E    FEL  K+   E     +  MV   
Sbjct: 208 LFDEM----PQRDLISWNTMLDGYARC---REMSKAFELFEKMP--ERNTVSWSTMVMGY 267

Query: 385 GKAGLVEGALKFIEEMPIEPNDIIWRTLLSACQNHENF--TIGELIAKHLMTMDSCNSSS 444
            KAG +E A    ++MP+   +++  T++ A    +        L+ + + +    ++++
Sbjct: 268 SKAGDMEMARVMFDKMPLPAKNVVTWTIIIAGYAEKGLLKEADRLVDQMVASGLKFDAAA 327

Query: 445 YVLLSNIYARLGLWSAASKVRMMMKKQNL 466
            + +       GL S   ++  ++K+ NL
Sbjct: 328 VISILAACTESGLLSLGMRIHSILKRSNL 346

HSP 4 Score: 41.6 bits (96), Expect = 1.7e-03
Identity = 70/357 (19.61%), Postives = 148/357 (41.46%), Query Frame = 1

Query: 120 LALELFEKMPERDLVSWNTIIGGFAKCGRIEFAHSLFNRMPKRDVISWSNMIDGYAKLGD 179
           L  ++  +    DL     +I   + C +   A  +FN++ + +V   +++I  +A+   
Sbjct: 38  LHAQIIRRNLHEDLHIAPKLISALSLCRQTNLAVRVFNQVQEPNVHLCNSLIRAHAQNSQ 97

Query: 180 IKVARTLFDEMPDKDVVAFN---TIMAGYAQNGYYTEALEIFHEMQRQSNLSPD---ETT 239
              A  +F EM    + A N     +        +   +++ H    +  LS D      
Sbjct: 98  PYQAFFVFSEMQRFGLFADNFTYPFLLKACSGQSWLPVVKMMHNHIEKLGLSSDIYVPNA 157

Query: 240 LVVALSAISQLGHVEKAASMHNYFLENGISVTGKVAVALIDMYSKCGSIENAILIFDGVD 299
           L+   S    LG V  A  +     E        +   L+    K G + +A  +FD + 
Sbjct: 158 LIDCYSRCGGLG-VRDAMKLFEKMSERDTVSWNSMLGGLV----KAGELRDARRLFDEMP 217

Query: 300 QKGIDHWNAMISGMARNGLGKLAFGMLLEMHRLSVKPDGITFIGVLNACAHAGLVKEGLI 359
           Q+ +  WN M+ G AR      AF +  +M   +     +++  ++   + AG ++   +
Sbjct: 218 QRDLISWNTMLDGYARCREMSKAFELFEKMPERNT----VSWSTMVMGYSKAGDMEMARV 277

Query: 360 CFELMRKVHKLEPKLQHYGCMVDILGKAGLVEGALKFIEEM---PIEPNDIIWRTLLSAC 419
            F+ M    K    +  +  ++    + GL++ A + +++M    ++ +     ++L+AC
Sbjct: 278 MFDKMPLPAK---NVVTWTIIIAGYAEKGLLKEADRLVDQMVASGLKFDAAAVISILAAC 337

Query: 420 QNHENFTIGELIAKHLMTMDSCNSSSYVL--LSNIYARLGLWSAASKVRMMMKKQNL 466
                 ++G  I   ++   +  S++YVL  L ++YA+ G    A  V   + K++L
Sbjct: 338 TESGLLSLGMRI-HSILKRSNLGSNAYVLNALLDMYAKCGNLKKAFDVFNDIPKKDL 381

BLAST of Cucsa.257610 vs. NCBI nr
Match: gi|449432255|ref|XP_004133915.1| (PREDICTED: pentatricopeptide repeat-containing protein At2g45350, chloroplastic [Cucumis sativus])

HSP 1 Score: 1020.8 bits (2638), Expect = 8.5e-295
Identity = 515/558 (92.29%), Postives = 515/558 (92.29%), Query Frame = 1

Query: 1   IRRNHLDDDPFLWNAVIKSYSHGNEPVRAL------------------------------ 60
           IRRNHLDDDPFLWNAVIKSYSHGNEPVRAL                              
Sbjct: 81  IRRNHLDDDPFLWNAVIKSYSHGNEPVRALVLFCMMLENGFCVDKFSFSLILKACARVCL 140

Query: 61  -------------LEIGSNLFLLNCLIAMYLRCGDIEFARQVFDRMPIQDSVSYNSMIDG 120
                        LEIGSNLFLLNCLIAMYLRCGDIEFARQVFDRMPIQDSVSYNSMIDG
Sbjct: 141 VEEGKQIHGLLMKLEIGSNLFLLNCLIAMYLRCGDIEFARQVFDRMPIQDSVSYNSMIDG 200

Query: 121 YVKSGTIDLARELFDSMPLEDKNLISWNSMLGGFAQTKDGIGLALELFEKMPERDLVSWN 180
           YVKSGTIDLARELFDSMPLEDKNLISWNSMLGGFAQTKDGIGLALELFEKMPERDLVSWN
Sbjct: 201 YVKSGTIDLARELFDSMPLEDKNLISWNSMLGGFAQTKDGIGLALELFEKMPERDLVSWN 260

Query: 181 TIIGGFAKCGRIEFAHSLFNRMPKRDVISWSNMIDGYAKLGDIKVARTLFDEMPDKDVVA 240
           TIIGGFAKCGRIEFAHSLFNRMPKRDVISWSNMIDGYAKLGDIKVARTLFDEMPDKDVVA
Sbjct: 261 TIIGGFAKCGRIEFAHSLFNRMPKRDVISWSNMIDGYAKLGDIKVARTLFDEMPDKDVVA 320

Query: 241 FNTIMAGYAQNGYYTEALEIFHEMQRQSNLSPDETTLVVALSAISQLGHVEKAASMHNYF 300
           FNTIMAGYAQNGYYTEALEIFHEMQRQSNLSPDETTLVVALSAISQLGHVEKAASMHNYF
Sbjct: 321 FNTIMAGYAQNGYYTEALEIFHEMQRQSNLSPDETTLVVALSAISQLGHVEKAASMHNYF 380

Query: 301 LENGISVTGKVAVALIDMYSKCGSIENAILIFDGVDQKGIDHWNAMISGMARNGLGKLAF 360
           LENGISVTGKVAVALIDMYSKCGSIENAILIFDGVDQKGIDHWNAMISGMARNGLGKLAF
Sbjct: 381 LENGISVTGKVAVALIDMYSKCGSIENAILIFDGVDQKGIDHWNAMISGMARNGLGKLAF 440

Query: 361 GMLLEMHRLSVKPDGITFIGVLNACAHAGLVKEGLICFELMRKVHKLEPKLQHYGCMVDI 420
           GMLLEMHRLSVKPDGITFIGVLNACAHAGLVKEGLICFELMRKVHKLEPKLQHYGCMVDI
Sbjct: 441 GMLLEMHRLSVKPDGITFIGVLNACAHAGLVKEGLICFELMRKVHKLEPKLQHYGCMVDI 500

Query: 421 LGKAGLVEGALKFIEEMPIEPNDIIWRTLLSACQNHENFTIGELIAKHLMTMDSCNSSSY 480
           LGKAGLVEGALKFIEEMPIEPNDIIWRTLLSACQNHENFTIGELIAKHLMTMDSCNSSSY
Sbjct: 501 LGKAGLVEGALKFIEEMPIEPNDIIWRTLLSACQNHENFTIGELIAKHLMTMDSCNSSSY 560

Query: 481 VLLSNIYARLGLWSAASKVRMMMKKQNLTKVPGCSWIELEGVVHEFLVRDKSHPYVSEIY 516
           VLLSNIYARLGLWSAASKVRMMMKKQNLTKVPGCSWIELEGVVHEFLVRDKSHPYVSEIY
Sbjct: 561 VLLSNIYARLGLWSAASKVRMMMKKQNLTKVPGCSWIELEGVVHEFLVRDKSHPYVSEIY 620

BLAST of Cucsa.257610 vs. NCBI nr
Match: gi|659075482|ref|XP_008438168.1| (PREDICTED: pentatricopeptide repeat-containing protein At2g45350, chloroplastic [Cucumis melo])

HSP 1 Score: 988.4 bits (2554), Expect = 4.7e-285
Identity = 496/558 (88.89%), Postives = 506/558 (90.68%), Query Frame = 1

Query: 1   IRRNHLDDDPFLWNAVIKSYSHGNEPVRAL------------------------------ 60
           IRR+HLDDDPFLWNAVIKSYSHGN+PVRAL                              
Sbjct: 81  IRRSHLDDDPFLWNAVIKSYSHGNDPVRALVLFCMMLENGFCVDKFSFSLILKACARVCL 140

Query: 61  -------------LEIGSNLFLLNCLIAMYLRCGDIEFARQVFDRMPIQDSVSYNSMIDG 120
                        LEIGSNLFLLNCLIAMYLRCGDIEFARQVFD MP+QDSVSYNSMIDG
Sbjct: 141 VEEGKQIHGLLMKLEIGSNLFLLNCLIAMYLRCGDIEFARQVFDTMPVQDSVSYNSMIDG 200

Query: 121 YVKSGTIDLARELFDSMPLEDKNLISWNSMLGGFAQTKDGIGLALELFEKMPERDLVSWN 180
           YVKSGTIDLARELFDSMPL DKNLISWNSMLGGFAQTKDGIGLALELFEKMPERDLVSWN
Sbjct: 201 YVKSGTIDLARELFDSMPLGDKNLISWNSMLGGFAQTKDGIGLALELFEKMPERDLVSWN 260

Query: 181 TIIGGFAKCGRIEFAHSLFNRMPKRDVISWSNMIDGYAKLGDIKVARTLFDEMPDKDVVA 240
           TIIGGFAKCGRIEFAHSLFNRMPKRDVISWSNMIDGYAKLGDIKVARTLFDEMPDKDVVA
Sbjct: 261 TIIGGFAKCGRIEFAHSLFNRMPKRDVISWSNMIDGYAKLGDIKVARTLFDEMPDKDVVA 320

Query: 241 FNTIMAGYAQNGYYTEALEIFHEMQRQSNLSPDETTLVVALSAISQLGHVEKAASMHNYF 300
           FNT+MAGYAQNGYYTEALEIFH+MQRQSNLSPDETTLVVALSAISQLGH+EKAASMHNYF
Sbjct: 321 FNTMMAGYAQNGYYTEALEIFHKMQRQSNLSPDETTLVVALSAISQLGHIEKAASMHNYF 380

Query: 301 LENGISVTGKVAVALIDMYSKCGSIENAILIFDGVDQKGIDHWNAMISGMARNGLGKLAF 360
           +ENGISVTGKVAVALIDMYSKCGSIENA LIFDGVDQKGIDHWNAMISGMARNGLGKLAF
Sbjct: 381 VENGISVTGKVAVALIDMYSKCGSIENAKLIFDGVDQKGIDHWNAMISGMARNGLGKLAF 440

Query: 361 GMLLEMHRLSVKPDGITFIGVLNACAHAGLVKEGLICFELMRKVHKLEPKLQHYGCMVDI 420
            ML+EMHRL VKPDGITFIGVLNACAHAGLVKEGLICFELMRKVHKLEPKLQHYGCMVDI
Sbjct: 441 EMLVEMHRLPVKPDGITFIGVLNACAHAGLVKEGLICFELMRKVHKLEPKLQHYGCMVDI 500

Query: 421 LGKAGLVEGALKFIEEMPIEPNDIIWRTLLSACQNHENFTIGELIAKHLMTMDSCNSSSY 480
           LGKAGLVEGALKFIEEMPIEPNDIIWRTLLSACQNHENF IGELIAKHLM MDSCNSSSY
Sbjct: 501 LGKAGLVEGALKFIEEMPIEPNDIIWRTLLSACQNHENFAIGELIAKHLMRMDSCNSSSY 560

Query: 481 VLLSNIYARLGLWSAASKVRMMMKKQNLTKVPGCSWIELEGVVHEFLVRDKSHPYVSEIY 516
           VLLSNIY+RLGLWSAASKVRM MKK+NLTKVPGCSWIELEGVVHEFLVRDKSHPYVSEIY
Sbjct: 561 VLLSNIYSRLGLWSAASKVRMTMKKKNLTKVPGCSWIELEGVVHEFLVRDKSHPYVSEIY 620

BLAST of Cucsa.257610 vs. NCBI nr
Match: gi|823186143|ref|XP_012489757.1| (PREDICTED: pentatricopeptide repeat-containing protein At2g45350, chloroplastic [Gossypium raimondii])

HSP 1 Score: 697.2 bits (1798), Expect = 2.2e-197
Identity = 331/547 (60.51%), Postives = 420/547 (76.78%), Query Frame = 1

Query: 7   DDDPFLWNAVIKSYSHGNEPVRAL------------------------------------ 66
           D+DPFLWNAVIKSYSHG E   A                                     
Sbjct: 84  DEDPFLWNAVIKSYSHGREAQEAFIILCLMLENGVPFDKFSLSLVLKACSQLGLLKEGMQ 143

Query: 67  -------LEIGSNLFLLNCLIAMYLRCGDIEFARQVFDRMPIQDSVSYNSMIDGYVKSGT 126
                  L  GS+LFL NCLI+ YLRCG I +ARQ+FDRM ++DSVSYNSMIDGY+K G 
Sbjct: 144 VHGLLRKLNFGSDLFLQNCLISYYLRCGFIGYARQLFDRMSMRDSVSYNSMIDGYIKRGM 203

Query: 127 IDLARELFDSMPLEDKNLISWNSMLGGFAQTKDGIGLALELFEKMPERDLVSWNTIIGGF 186
           IDLA+ELFD MPLE KNLI+WNSM+ G+ Q KDG+GLAL LFEKMP+RDL+SWN++I G+
Sbjct: 204 IDLAKELFDVMPLEKKNLITWNSMISGYTQLKDGMGLALGLFEKMPDRDLISWNSMINGY 263

Query: 187 AKCGRIEFAHSLFNRMPKRDVISWSNMIDGYAKLGDIKVARTLFDEMPDKDVVAFNTIMA 246
            KCG +E A  LF++MP+ DV+SW+NMI+GYAK+G I +AR+LF+EM ++D+V  N +MA
Sbjct: 264 VKCGNMEDAQILFDKMPRWDVVSWANMINGYAKVGKIDLARSLFNEMSERDIVVCNAMMA 323

Query: 247 GYAQNGYYTEALEIFHEMQRQSNLSPDETTLVVALSAISQLGHVEKAASMHNYFLENGIS 306
           G  QNGY  EALEIF+ +QR  +L PD  TL++ALSAI+QLGHV+K  ++H+Y  +   S
Sbjct: 324 GCVQNGYCGEALEIFYHLQRNCSLQPDSATLLIALSAIAQLGHVDKGLALHHYLKQKKFS 383

Query: 307 VTGKVAVALIDMYSKCGSIENAILIFDGVDQKGIDHWNAMISGMARNGLGKLAFGMLLEM 366
           + G++ VALIDMYSKCGSI++A+L+F+G+  K +DHWNAMI G+A +G+G+LAF +L++M
Sbjct: 384 LGGRLGVALIDMYSKCGSIKDAVLVFEGIKYKTVDHWNAMIGGLAIHGMGELAFKLLMDM 443

Query: 367 HRLSVKPDGITFIGVLNACAHAGLVKEGLICFELMRKVHKLEPKLQHYGCMVDILGKAGL 426
            +L V+PD IT+IGV+NAC HAGLVKEGL+CF++MR+VHK+ PKLQHYGCMVDILG+AG 
Sbjct: 444 EKLCVEPDDITYIGVMNACGHAGLVKEGLVCFDIMRRVHKMVPKLQHYGCMVDILGRAGQ 503

Query: 427 VEGALKFIEEMPIEPNDIIWRTLLSACQNHENFTIGELIAKHLMTMDSCNSSSYVLLSNI 486
           ++ A KFIEEMPIE ND+IWR+LLSAC+NHEN  +GE  AKHL+T+DSCNSSSYVLLSN+
Sbjct: 504 IDVARKFIEEMPIEANDVIWRSLLSACKNHENIDVGEPAAKHLITLDSCNSSSYVLLSNM 563

Query: 487 YARLGLWSAASKVRMMMKKQNLTKVPGCSWIELEGVVHEFLVRDKSHPYVSEIYSVLDGF 511
           YA LG+W+A S VR+MMK++NL K+PGCSWIELEG VHEF V+DKSHP V EIYS+LD  
Sbjct: 564 YAGLGMWNAVSSVRLMMKERNLKKIPGCSWIELEGTVHEFFVQDKSHPQVMEIYSLLDSL 623

BLAST of Cucsa.257610 vs. NCBI nr
Match: gi|590723036|ref|XP_007052071.1| (Pentatricopeptide repeat (PPR) superfamily protein, putative [Theobroma cacao])

HSP 1 Score: 693.7 bits (1789), Expect = 2.4e-196
Identity = 327/548 (59.67%), Postives = 419/548 (76.46%), Query Frame = 1

Query: 9   DPFLWNAVIKSYSHGNEPVRAL-------------------------------------- 68
           DPFLWNA IKSYSHG +  +A                                       
Sbjct: 86  DPFLWNAAIKSYSHGRDAKQAFVMLCLMLENGVRFDTFSLSLVLKACSRLGLMKEGMQVH 145

Query: 69  -----LEIGSNLFLLNCLIAMYLRCGDIEFARQVFDRMPIQDSVSYNSMIDGYVKSGTID 128
                L  GS+LFL NCLI++Y+RCG + +ARQ+FDRM  +DSVSYNSMIDGY+K+G I 
Sbjct: 146 GLLRKLNFGSDLFLQNCLISLYIRCGFVGYARQLFDRMCRRDSVSYNSMIDGYIKTGMIH 205

Query: 129 LARELFDSMPLEDKNLISWNSMLGGFAQTKDGIGLALELFEKMPERDLVSWNTIIGGFAK 188
           LARELF+ MP+EDKNLI+WNSM+ G+AQ + G+ +AL+LFE+MP+RDL+SWN++I GF K
Sbjct: 206 LARELFEFMPMEDKNLITWNSMISGYAQLEYGMVMALKLFEEMPDRDLISWNSMINGFVK 265

Query: 189 CGRIEFAHSLFNRMPKRDVISWSNMIDGYAKLGDIKVARTLFDEMPDKDVVAFNTIMAGY 248
           CG +E A +LF +MPKRDV+SW+NMI+GYAK+G I +AR LFDEMP++DVV  N +M GY
Sbjct: 266 CGNMEDAQALFVKMPKRDVVSWANMINGYAKVGKIDLARRLFDEMPERDVVVCNAMMGGY 325

Query: 249 AQNGYYTEALEIFHEMQRQSNLSPDETTLVVALSAISQLGHVEKAASMHNYFLENGISVT 308
            QNGY +EALEIF+ +QR  NL PD  TL++ LSAI+QLG ++K  ++H Y  EN  S++
Sbjct: 326 VQNGYCSEALEIFYHLQRYDNLQPDNATLLIVLSAIAQLGRIDKGLAIHQYLEENKFSLS 385

Query: 309 GKVAVALIDMYSKCGSIENAILIFDGVDQKGIDHWNAMISGMARNGLGKLAFGMLLEMHR 368
           G++ VALIDMYSKCGS++N +L+F+ ++ + +DHWNAMI G+A +GLG+LAF +L+EM R
Sbjct: 386 GRLGVALIDMYSKCGSVKNGMLVFESIEDRSVDHWNAMIGGLAIHGLGELAFSLLIEMER 445

Query: 369 LSVKPDGITFIGVLNACAHAGLVKEGLICFELMRKVHKLEPKLQHYGCMVDILGKAGLVE 428
           LSV+PD IT+IGVLNAC HAGLVKEGLICF++MR+VHK+ PKL+HYGC+VDILG+AG + 
Sbjct: 446 LSVEPDDITYIGVLNACGHAGLVKEGLICFDIMRRVHKMVPKLKHYGCIVDILGRAGQIA 505

Query: 429 GALKFIEEMPIEPNDIIWRTLLSACQNHENFTIGELIAKHLMTMDSCNSSSYVLLSNIYA 488
            A KFIEEMPIE ND+IWR+LLSAC+NHEN  +GE +A+HL+++DSCNSSSY+LLSN+YA
Sbjct: 506 EARKFIEEMPIEANDVIWRSLLSACKNHENIDVGEPVAEHLISLDSCNSSSYILLSNMYA 565

Query: 489 RLGLWSAASKVRMMMKKQNLTKVPGCSWIELEGVVHEFLVRDKSHPYVSEIYSVLDGFGA 514
            LG+WSA S VRM MK++NL K+PGCSWIELEG VHEF V+DKSHP V EIYS+L  F  
Sbjct: 566 GLGMWSAVSNVRMRMKERNLKKIPGCSWIELEGTVHEFFVQDKSHPQVMEIYSLLGSFSI 625

BLAST of Cucsa.257610 vs. NCBI nr
Match: gi|225453947|ref|XP_002274056.1| (PREDICTED: pentatricopeptide repeat-containing protein At2g45350, chloroplastic [Vitis vinifera])

HSP 1 Score: 692.6 bits (1786), Expect = 5.3e-196
Identity = 329/556 (59.17%), Postives = 423/556 (76.08%), Query Frame = 1

Query: 2   RRNHLDDDPFLWNAVIKSYSHGNEPVRAL------------------------------- 61
           R++   D+PFLWNA+IKS+SHG +P  A                                
Sbjct: 79  RKHRKQDNPFLWNAIIKSFSHGEDPREAFVIFNLMLENGVCVDKFSFSLVLKACSRLGLI 138

Query: 62  ------------LEIGSNLFLLNCLIAMYLRCGDIEFARQVFDRMPIQDSVSYNSMIDGY 121
                       +EIGS++FL NCL+ +YLRCG +  ARQ+FDRM  +DSVS+NSMIDGY
Sbjct: 139 KEGMQIHGLLGRMEIGSDVFLQNCLMCLYLRCGCLGIARQLFDRMMKRDSVSFNSMIDGY 198

Query: 122 VKSGTIDLARELFDSMPLEDKNLISWNSMLGGFAQTKDGIGLALELFEKMPERDLVSWNT 181
           VK G +  ARELFD MP+E KNLISWNSM+ G+A++++G+ +A ELFE+MP+RDL+SWN+
Sbjct: 199 VKHGMVKSARELFDVMPMEQKNLISWNSMISGYARSEEGLRVAWELFEEMPKRDLISWNS 258

Query: 182 IIGGFAKCGRIEFAHSLFNRMPKRDVISWSNMIDGYAKLGDIKVARTLFDEMPDKDVVAF 241
           +I G  KCG++E AH LFN+MPKRDV+SW+NM+DGYAKLG+I +AR LFDEMP++DV++ 
Sbjct: 259 MIDGCVKCGKMENAHHLFNQMPKRDVVSWANMVDGYAKLGEIDIARGLFDEMPERDVISC 318

Query: 242 NTIMAGYAQNGYYTEALEIFHEMQRQSNLSPDETTLVVALSAISQLGHVEKAASMHNYFL 301
           N +MAGY QNG+  EAL  FH+M  +  L PD  TL++ LSAI+QLGH ++  ++H Y  
Sbjct: 319 NAMMAGYVQNGHLMEALNFFHDMLSRKELFPDNATLLITLSAIAQLGHFDEGVALHCYIE 378

Query: 302 ENGISVTGKVAVALIDMYSKCGSIENAILIFDGVDQKGIDHWNAMISGMARNGLGKLAFG 361
           +NG S++ K+ VALIDMY+KCGSI+NA+ +F+ +D K IDHWNA+I G+A +GLG++AF 
Sbjct: 379 DNGFSLSEKLGVALIDMYAKCGSIDNALSVFEDIDDKSIDHWNAIIGGLAIHGLGEVAFE 438

Query: 362 MLLEMHRLSVKPDGITFIGVLNACAHAGLVKEGLICFELMRKVHKLEPKLQHYGCMVDIL 421
           + +EM +L VKPD ITFIGVLNAC HAGLVKEGL+CFELMR+VHK+EPKLQHYGCMVDIL
Sbjct: 439 LFMEMEKLFVKPDDITFIGVLNACNHAGLVKEGLMCFELMRRVHKVEPKLQHYGCMVDIL 498

Query: 422 GKAGLVEGALKFIEEMPIEPNDIIWRTLLSACQNHENFTIGELIAKHLMTMDSCNSSSYV 481
           G+AG VE A KF+E+MPIEPND++WRTLLSAC+NHENFTIGE +AKHL+++DS N SSYV
Sbjct: 499 GRAGHVEEAKKFVEKMPIEPNDVVWRTLLSACRNHENFTIGEPVAKHLISVDSYNPSSYV 558

Query: 482 LLSNIYARLGLWSAASKVRMMMKKQNLTKVPGCSWIELEGVVHEFLVRDKSHPYVSEIYS 515
           LLSNIYA  G+W+   ++RMMMK+++L K+PGCS IELEG VHEF VRDKSHP V EIYS
Sbjct: 559 LLSNIYAGFGMWNDVYRIRMMMKQRDLKKIPGCSQIELEGNVHEFFVRDKSHPQVREIYS 618