Cucsa.256650 (gene) Cucumber (Gy14) v1

BLAST of Cucsa.256650 vs. Swiss-Prot
Match: DNLI1_DICDI (DNA ligase 1 OS=Dictyostelium discoideum GN=lig1 PE=3 SV=1)

HSP 1 Score: 73.9 bits (180), Expect = 5.2e-12
Identity = 69/294 (23.47%), Postives = 140/294 (47.62%), Query Frame = 1

Query: 19  EDLDLLKKDKHKKKRKKENRDHKEKKKSRSRHKRDKEKDRVKDKAKTLDESKLPDKIQCH 78
           E+++L + +K K K+KK++   KE K+S+S     K  +  + K      SK  + ++  
Sbjct: 106 EEMELDENEKEKNKKKKQSLKTKENKESKSSSSSKKTIENKETKKPEKQSSKQSNNLKRL 165

Query: 79  NRQFLFQKDLEDKDNCRLTEKRYAGQSAGYSDGKFSCGSHLPEKFSVSEIALELPKRNKD 138
            R+ +   + +++D  +  +          SD +    S   +++   ++  +  K+ K+
Sbjct: 166 KRKKMDDDEEDEEDENKTDDNDLDDMLDDDSDNEKDSISSKDKEYK-EKVLKDKEKKEKE 225

Query: 139 EDVDSGFRNLSGEKLFACLPEKDDWMDKTMAKVTQNFNEGKTKNKNKDSREAKYGDLGIQ 198
                               +K+  + +  +K  +   + K + + KD +E +  +  ++
Sbjct: 226 --------------------KKEKELKEKESKEKEKKEKEKKEKEEKDKKEKELKEKELK 285

Query: 199 E-ESKFRKRGTIQPMVKDGVDSVEKNADAKTEEKKWKGNGRDEKSKDKQKDKDKEGKGKD 258
           E E K +K   ++   K+  D  +K  + K +EKK K    +EK K+K++ K+KE K K+
Sbjct: 286 EKELKDKKEKELKEKEKELKDKEKKEKELKEKEKKEK----EEKEKEKKEKKEKELKEKE 345

Query: 259 KERDKKKKKKEKIEQKFGNLKKSKERYEGDVTSP--DSIITSQLLRDSHGSVAS 310
           ++  K+K+ KEK E K   LK+ KE  E ++TSP  ++I  S L + ++    S
Sbjct: 346 EKEKKEKELKEK-ELKEKELKE-KELKEKELTSPKKETIDISDLFKRANAEAKS 372

HSP 2 Score: 57.4 bits (137), Expect = 5.0e-07
Identity = 65/287 (22.65%), Postives = 117/287 (40.77%), Query Frame = 1

Query: 14  KKARIEDLDLLKKDKHKKKRKKENRDHKEKKKSRSRHKRDKEKDRVKDKAKTLDESKLPD 73
           K+  ++D +  +K+K +K+ K++    KEKK+   + K +K+K   + K K L E +L D
Sbjct: 211 KEKVLKDKEKKEKEKKEKELKEKESKEKEKKEKEKKEKEEKDKKEKELKEKELKEKELKD 270

Query: 74  KIQCHNRQFLFQKDLEDKD--NCRLTEKRYAGQSAGYSDGKFSCGSHLPEKFSVSEIALE 133
           K +   ++   +K+L+DK+     L EK    +     + K      L EK    +   E
Sbjct: 271 KKEKELKE--KEKELKDKEKKEKELKEKEKKEKEEKEKEKKEKKEKELKEKEEKEKKEKE 330

Query: 134 LPKRNKDE---------------------DVDSGFRNLSGEKLFACLPEKDDWMDKTMAK 193
           L ++   E                     D+   F+  + E   + +P       KT  K
Sbjct: 331 LKEKELKEKELKEKELKEKELTSPKKETIDISDLFKRANAEAK-SSVPTSTSKNSKTNKK 390

Query: 194 VTQNFNEGKTKNKNKDSREAKYGDLGIQEESKFRKRGTIQPMVKDGVDSVEKNADAKTEE 253
              +     T  K     EAK        +S      T        + S   ++ +K EE
Sbjct: 391 QKVDHKPTATTKKPSPVLEAK--------QSTTTTTTTTTTSTATTISSKSISSPSKKEE 450

Query: 254 ------KKWKGNGRDEKSKDKQKDKDKEGKGKDKERDKKKKKKEKIE 272
                 KK     + E  K+K+K+K+KE +  ++E ++++   EK+E
Sbjct: 451 KEVITSKKQVEATKVEVKKEKEKEKEKEKEDDEEEEEEEEDDDEKLE 486

HSP 3 Score: 50.1 bits (118), Expect = 8.0e-05
Identity = 56/265 (21.13%), Postives = 122/265 (46.04%), Query Frame = 1

Query: 13  EKKARIEDLDLLKKD---KHKKKRKKENRDHKEKKKSRSRHKRDKEKDRVKDKAKTLDES 72
           EK+  ++D +  +K+   K KK+++++ ++ KEKK+   + K +KEK     K K L E 
Sbjct: 278 EKEKELKDKEKKEKELKEKEKKEKEEKEKEKKEKKEKELKEKEEKEK-----KEKELKEK 337

Query: 73  KLPDKIQCHNRQFLFQKDLEDKDNCRLTEKRYAGQSAGYSDGKFSCGSHLPEKFSVSEIA 132
           +L +K        L +K+L++K+   LT  +   ++   SD      ++   K SV    
Sbjct: 338 ELKEK-------ELKEKELKEKE---LTSPK--KETIDISD--LFKRANAEAKSSVPTST 397

Query: 133 LELPKRNKDEDVDSGFRNLSGEKLFACLPEKDDWMDKTMAKVTQNFNEGKTKNKNKDSRE 192
            +  K NK + VD      + +K    L  K      T    T       +K+ +  S++
Sbjct: 398 SKNSKTNKKQKVDHK-PTATTKKPSPVLEAKQSTTTTTTTTTTSTATTISSKSISSPSKK 457

Query: 193 AKYGDLGIQEESKFRKRGTIQPMVKDGVDSVEKNADAKTEEKKWKGNGRDEKSKDKQKDK 252
            +   +  +++ +  K    +   K+     E + + + EE+       D   ++ ++++
Sbjct: 458 EEKEVITSKKQVEATKVEVKKEKEKEKEKEKEDDEEEEEEEEDDDEKLEDIDEEEYEEEE 517

Query: 253 DKEGKGKDKERDKKKKKKEKIEQKF 275
           +++ +G  +  ++++KK  +I+ KF
Sbjct: 518 EEDEEGISENEEEEEKKSTQIKSKF 522

BLAST of Cucsa.256650 vs. Swiss-Prot
Match: GARP_PLAFF (Glutamic acid-rich protein OS=Plasmodium falciparum (isolate FC27 / Papua New Guinea) GN=GARP PE=4 SV=1)

HSP 1 Score: 65.9 bits (159), Expect = 1.4e-09
Identity = 66/268 (24.63%), Postives = 115/268 (42.91%), Query Frame = 1

Query: 33  RKKENRDHKEKKKSRSRHKRD-KEKDRVKDKAKTLDESKLPDKIQCHNRQFLFQKDLEDK 92
           +  EN   K+K K   +HK+D KEK   KDK +  D+     K + H ++   +KD + K
Sbjct: 111 KDNENSVDKKKDKKEKKHKKDKKEKKEKKDKKEKKDK-----KEKKHKKEKKHKKDKKKK 170

Query: 93  DNCRLTEKRYAGQSAGYSDGKFSCGSHLPEKFSVSEIALELPKRNKDEDVDSGFRNLSGE 152
           +N  +                +  G H P+       A E  + N DE++ S   N +  
Sbjct: 171 ENSEVM-------------SLYKTGQHKPKN------ATEHGEENLDEEMVSEINNNAQG 230

Query: 153 KLFACLP--EKDDWMDKTMAKVTQNFNEGKTKNKNKDSREAKYGDLGIQEESKFRKRGTI 212
            L    P   ++      ++ V +  N+ K  +K   S + K      Q+E   +     
Sbjct: 231 GLLLSSPYQYREQGGCGIISSVHETSNDTKDNDKENISEDKKEDH---QQEEMLKTLDKK 290

Query: 213 QPMVKDGVDSVEKNADAKTEEKKWKGNGRDEKSKDKQKDKDKEGKGKDKERDKKKKKK-- 272
           +   K+     E     K E+KK K   +++K ++K++ K ++ + K KE++ KK+KK  
Sbjct: 291 ERKQKE----KEMKEQEKIEKKKKKQEEKEKKKQEKERKKQEKKERKQKEKEMKKQKKIE 347

Query: 273 ---EKIEQKFGNLKKSKERYEGDVTSPD 293
              +K E+K    KK  +  E  +  PD
Sbjct: 351 KERKKKEEKEKKKKKHDKENEETMQQPD 347

HSP 2 Score: 55.8 bits (133), Expect = 1.5e-06
Identity = 61/276 (22.10%), Postives = 127/276 (46.01%), Query Frame = 1

Query: 25  KKDKHKKKRKKENRDHKEKKKSRSRHKRDKEKDRVKDKAKTLDESKLPDKIQCHNRQFLF 84
           KK++HK K  K      + KK + +HK+ K++   K   K + E +  D ++  N     
Sbjct: 405 KKEEHKSKEHKSKGKKDKGKKDKGKHKKAKKEKVKKHVVKNVIEDEDKDGVEIIN----- 464

Query: 85  QKDLEDKDNCRLTEKRYAGQSAGYSDGKFSCGSHLPEKFSVSEIALELPKRNKDEDVDSG 144
              LEDK+ C   E+    +S   S  +       PE+       L L  ++K E+    
Sbjct: 465 ---LEDKEACE--EQHITVESRPLSQPQCKLIDE-PEQ-------LTLMDKSKVEE---- 524

Query: 145 FRNLS-GEKLFACL------PEKDDWMDKTMAKVTQ-NFNEGKTKNKNKDSREAKYGDLG 204
            +NLS  E+L   +      P +D+   K MAK+ +    + K  +K +D +E       
Sbjct: 525 -KNLSIQEQLIGTIGRVNVVPRRDN-HKKKMAKIEEAELQKQKHVDKEEDKKEE---SKE 584

Query: 205 IQEESKFRKRGTIQPMVKDGVDSVEKNADAKTEEKKWKGNGRDEKSKDKQKDKDKEGKGK 264
           +QEESK          V++  + VE++ + + EE++ +    +E+ +++++++++E    
Sbjct: 585 VQEESK---------EVQEDEEEVEEDEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEDED 644

Query: 265 DKERDKKKKKKEKIEQKFGNLKKSKERYEGDVTSPD 293
           +++ D  ++ ++  E+   + ++  +  + D    D
Sbjct: 645 EEDEDDAEEDEDDAEEDEDDAEEDDDEEDDDEEDDD 644

HSP 3 Score: 47.8 bits (112), Expect = 4.0e-04
Identity = 64/300 (21.33%), Postives = 118/300 (39.33%), Query Frame = 1

Query: 13  EKKARIEDLDLL-KKDKHKKKRKKENRDHKEKKKSRS-------RHKRDKEKDRVKDKAK 72
           +KK + E  D   KKDK +KK KKE +  K+KKK  +       +  + K K+  +   +
Sbjct: 131 DKKEKKEKKDKKEKKDKKEKKHKKEKKHKKDKKKKENSEVMSLYKTGQHKPKNATEHGEE 190

Query: 73  TLDESKLPDKIQCHNRQFLFQKDLE--DKDNCRLTEKRYAGQSAGYSDGKFSCGSHLPEK 132
            LDE  + +         L     +  ++  C +    +   +    + K +      E 
Sbjct: 191 NLDEEMVSEINNNAQGGLLLSSPYQYREQGGCGIISSVHETSNDTKDNDKENISEDKKED 250

Query: 133 FSVSEIALELPKRNKDEDVDSGFRNLSGEKLFACLPEKDDWMD----KTMAKVTQNFNEG 192
               E+   L K+ + +           EK      EK+        K   K  +   E 
Sbjct: 251 HQQEEMLKTLDKKERKQKEKEMKEQEKIEKKKKKQEEKEKKKQEKERKKQEKKERKQKEK 310

Query: 193 KTKNKNKDSREAKYGDLGIQEESKFRKRGTIQPMVKDGVDSVEKN-----------ADAK 252
           + K + K  +E K  +   +++ K  K    + M +    S E N            D  
Sbjct: 311 EMKKQKKIEKERKKKEEKEKKKKKHDKENE-ETMQQPDQTSEETNNEIMVPLPSPLTDVT 370

Query: 253 TEEKKWKGNGRDEKSKDKQKDKDKEGKGKDKERDKKKKKKEKIEQKFGNLKKSKERYEGD 288
           T E+  +G  ++E+ K+ +    KEG+ K++E  +++ KKE+ + K    K  K++ + D
Sbjct: 371 TPEEHKEGEHKEEEHKEGEH---KEGEHKEEEHKEEEHKKEEHKSKEHKSKGKKDKGKKD 426

HSP 4 Score: 39.3 bits (90), Expect = 1.4e-01
Identity = 68/303 (22.44%), Postives = 119/303 (39.27%), Query Frame = 1

Query: 19  EDLDLLKKDKHKKK-----------RKKENRDHKEKKKSRSRHKRDKEKDRVKDKAKTLD 78
           E L  L K + K+K           +KK+ ++ KEKKK     K+ ++K+R K K K + 
Sbjct: 255 EMLKTLDKKERKQKEKEMKEQEKIEKKKKKQEEKEKKKQEKERKKQEKKER-KQKEKEMK 314

Query: 79  ESKLPDKIQCHNRQFLFQKDLEDKDNCRLTEKRYAGQSAGYSDGKFSCGSHLPEKFSVSE 138
           + K  +K +    +   +K   DK+N    ++         ++      S L +  +  E
Sbjct: 315 KQKKIEKERKKKEEKEKKKKKHDKENEETMQQPDQTSEETNNEIMVPLPSPLTDVTTPEE 374

Query: 139 IALELPKRNKDEDVDSGFRNLSGEKLFACLPEKDDWMDKTMAKVTQNFNEGKTKNKNKDS 198
                   +K+E+   G  +  GE       ++++  ++   K      E K+K K KD 
Sbjct: 375 ---HKEGEHKEEEHKEG-EHKEGEH------KEEEHKEEEHKKEEHKSKEHKSKGK-KDK 434

Query: 199 REAKYGDLGIQEESKFRK---RGTIQPMVKDGVDSVEKNADAKTEEKKWKGNGR------ 258
            +   G     ++ K +K   +  I+   KDGV+ +        EE+      R      
Sbjct: 435 GKKDKGKHKKAKKEKVKKHVVKNVIEDEDKDGVEIINLEDKEACEEQHITVESRPLSQPQ 494

Query: 259 ----DEKSK----DKQKDKDKEGKGKDK------------ERDKKKKKKEKIEQKFGNLK 282
               DE  +    DK K ++K    +++             RD  KKK  KIE+    L+
Sbjct: 495 CKLIDEPEQLTLMDKSKVEEKNLSIQEQLIGTIGRVNVVPRRDNHKKKMAKIEE--AELQ 543

HSP 5 Score: 36.6 bits (83), Expect = 9.1e-01
Identity = 63/298 (21.14%), Postives = 122/298 (40.94%), Query Frame = 1

Query: 13  EKKARIEDLDLLKKDKHKKKRKKENRDHKEKKKSRSRHKRDKEKDRVKDKAKTLDESKLP 72
           EKK R +    +KK   +KK +KE +  +EK+K + +H ++ E + ++   +T +E+   
Sbjct: 301 EKKERKQKEKEMKK---QKKIEKERKKKEEKEKKKKKHDKENE-ETMQQPDQTSEETNNE 360

Query: 73  DKIQCHNRQFLFQKDLEDKDNCRLTEKRYAGQSAGYSDGKFSCGSHLPEKFSVSEIALEL 132
             +   +         E K+     E+        + +G+   G H  E+    E   E 
Sbjct: 361 IMVPLPSPLTDVTTPEEHKEGEHKEEE--------HKEGEHKEGEHKEEEHKEEEHKKE- 420

Query: 133 PKRNKDEDVDSGFRNLSGEKLFACLPEKDDWMDKTMAKVTQNFNEGKTKNKNKDSRE-AK 192
              +K ++  S  +   G+K      +K         KV ++  +   ++++KD  E   
Sbjct: 421 --EHKSKEHKSKGKKDKGKK------DKGKHKKAKKEKVKKHVVKNVIEDEDKDGVEIIN 480

Query: 193 YGDLGIQEESKF--RKRGTIQPMVK--DGVDSVEKNADAKTEEKKW-------------- 252
             D    EE       R   QP  K  D  + +     +K EEK                
Sbjct: 481 LEDKEACEEQHITVESRPLSQPQCKLIDEPEQLTLMDKSKVEEKNLSIQEQLIGTIGRVN 540

Query: 253 ----KGNGRDEKSKDKQKDKDKEGKGKDKERDKKKKKKEKIEQKFGNLKKSKERYEGD 288
               + N + + +K ++ +  K+ K  DKE DKK++ KE ++++   +++ +E  E D
Sbjct: 541 VVPRRDNHKKKMAKIEEAELQKQ-KHVDKEEDKKEESKE-VQEESKEVQEDEEEVEED 575

BLAST of Cucsa.256650 vs. Swiss-Prot
Match: CYLC1_BOVIN (Cylicin-1 OS=Bos taurus GN=CYLC1 PE=1 SV=1)

HSP 1 Score: 64.3 bits (155), Expect = 4.1e-09
Identity = 78/302 (25.83%), Postives = 125/302 (41.39%), Query Frame = 1

Query: 6   PFPPPGYEKKARIEDLDLLKKDKHKKK-RKKENRDHKEKKKSRSRHKRDKEKDRVKDKAK 65
           P+    + KKA  +     KK+   KK  KK+   H+  +K + R+K DK   +    ++
Sbjct: 121 PYTHITHSKKAESKKYKDDKKETALKKISKKDTGPHEVDEKPKRRNKADKTPSKSSHGSQ 180

Query: 66  TLDESKLPDKIQCHNRQFLFQKDLEDKDNCRLTEKRYAGQSAGYSDGKFSCGSHLPEKFS 125
              +SK   +    ++  +       K      EKRY+  S      +    S   +K+S
Sbjct: 181 LSKKSKSKSETNPESKDSISVSIKHQKK-----EKRYSKDSK-----EMDFESTSTKKYS 240

Query: 126 VSEIALELPKRNKDEDVDSGFRNLSGEKLFACLPEKDDW---MDKTMAKVTQNFNEGKTK 185
            S       K N D   ++  +N S   L   L E D      D  +   +QN ++  TK
Sbjct: 241 KSS------KNNSDAVSETCSKNSSNVGLMVHLGESDAESMEFDMWLKNYSQNNSKKPTK 300

Query: 186 NKNKDSREAKYGDLGI--QEESKFRKRGTIQPMVKDGVDSVEKNADAKTEEKKWKGNGRD 245
              K   + K  D      +++K  K+G  +   K G     ++ DA++ + K    G+ 
Sbjct: 301 KDAKKDAKGKGSDAESVDSKDAKKDKKGATKD-TKKGAKKDTESTDAESGDSKDAKKGKK 360

Query: 246 EKSKDKQKDKDKE-------GKGKDKERDKKKKKKE-KIEQKFGNLKKSKERYEGDVTSP 294
           E  KDK+KD  K+       G  KD ++D KK KK+ K + K  + KK  E    D  S 
Sbjct: 361 ESKKDKKKDAKKDAASDAESGDSKDAKKDSKKGKKDSKKDNKKKDAKKDAE--STDAESG 403

HSP 2 Score: 48.9 bits (115), Expect = 1.8e-04
Identity = 78/347 (22.48%), Postives = 134/347 (38.62%), Query Frame = 1

Query: 6   PFPPPGYEKKARIEDLDLLKKDKHKKK-RKKENRDHKEKKKSRSRHKRDKEKDRVKDKAK 65
           P+    + KKA  +     KK+   KK  KK+   H+  +K + R+K DK   +    ++
Sbjct: 121 PYTHITHSKKAESKKYKDDKKETALKKISKKDTGPHEVDEKPKRRNKADKTPSKSSHGSQ 180

Query: 66  TLDESKLPDKIQCHNRQFLFQKDLEDKDNCRLTEKRYAGQSAGYSDGKFSCGSHLPEKFS 125
              +SK   +    ++  +       K      EKRY+  S      +    S   +K+S
Sbjct: 181 LSKKSKSKSETNPESKDSISVSIKHQKK-----EKRYSKDSK-----EMDFESTSTKKYS 240

Query: 126 VSEIALELPKRNKDEDVDSGFRNLSGEKLFACLPEKDDW---MDKTMAKVTQNFNEGKTK 185
            S       K N D   ++  +N S   L   L E D      D  +   +QN ++  TK
Sbjct: 241 KSS------KNNSDAVSETCSKNSSNVGLMVHLGESDAESMEFDMWLKNYSQNNSKKPTK 300

Query: 186 NKNK-------------DSREAKYGDLGIQEESKFRKRGTIQPMVKDGVDSVEKNADAKT 245
              K             DS++AK    G  +++K   +   +    +  DS +     K 
Sbjct: 301 KDAKKDAKGKGSDAESVDSKDAKKDKKGATKDTKKGAKKDTESTDAESGDSKDAKKGKKE 360

Query: 246 EEKKWKGNGRDEKSKDKQ-------KDKDKEGKGKDKERDKKKK--KK--EKIEQKFGNL 305
            +K  K + + + + D +       K   K+GK KD ++D KKK  KK  E  + + G+ 
Sbjct: 361 SKKDKKKDAKKDAASDAESGDSKDAKKDSKKGK-KDSKKDNKKKDAKKDAESTDAESGDS 420

Query: 306 KKS-KERYEGDVTSPDSIITSQLLRDSHGSVASIGSLMKRKEFENNG 324
           K + K+  +G   S          +D+  + A  G     K+    G
Sbjct: 421 KDAKKDSKKGKKDSKKDDKKKDAKKDAESTDAESGDSKNAKKDSKKG 450

HSP 3 Score: 47.0 bits (110), Expect = 6.8e-04
Identity = 66/273 (24.18%), Postives = 114/273 (41.76%), Query Frame = 1

Query: 43  KKKSRSRHKRDKEKDRVKD-KAKTLDESKLPDKIQCHNRQFLFQKDLEDKDNCRLTEKRY 102
           K  S++  K+  +KD  KD K K  D   +  K              +DK       K+ 
Sbjct: 272 KNYSQNNSKKPTKKDAKKDAKGKGSDAESVDSK-----------DAKKDKKGATKDTKKG 331

Query: 103 AGQSAGYSDGKFSCGSHLPEKFSVSEIALELPKRNKDEDVDSGFRNLSGEKLFACLPEKD 162
           A +    +D +   G     K    E   +   + KD   D+     SG+   A   +KD
Sbjct: 332 AKKDTESTDAE--SGDSKDAKKGKKESKKD---KKKDAKKDAASDAESGDSKDA---KKD 391

Query: 163 DWMDKTMAKVTQNFNEGKTKNKNKDSREAKYGDL-GIQEESKFRKRGTIQP-----MVKD 222
               K   K ++  N+ K   K+ +S +A+ GD    +++SK  K+ + +        KD
Sbjct: 392 SKKGK---KDSKKDNKKKDAKKDAESTDAESGDSKDAKKDSKKGKKDSKKDDKKKDAKKD 451

Query: 223 GVDSVEKNADAKTEEKKWKGNGRDEKSKDKQKDK-----DKEGKGKDKERDKKKKKKEKI 282
              +  ++ D+K  +K  K   +D+K KD +KD      D E +G D ++ KK  KK+K 
Sbjct: 452 AESTDAESGDSKNAKKDSKKGKKDDKKKDAKKDAVSTDADSESEG-DAKKSKKDSKKDKK 511

Query: 283 EQKFGNLKKSKERYEGDVTSPDSIITSQLLRDS 304
           + K  + KK   + +    +     + ++ RDS
Sbjct: 512 DLKKDDQKKPAMKSKESTETESDWESKKVKRDS 521

HSP 4 Score: 45.8 bits (107), Expect = 1.5e-03
Identity = 67/292 (22.95%), Postives = 120/292 (41.10%), Query Frame = 1

Query: 28  KHKKKRKKENRDHKEKK-KSRSRHKRDKEKDRVKDK-AKTLDESKLPDKIQCH------- 87
           KH+KK K+ ++D KE   +S S  K  K      D  ++T  ++     +  H       
Sbjct: 204 KHQKKEKRYSKDSKEMDFESTSTKKYSKSSKNNSDAVSETCSKNSSNVGLMVHLGESDAE 263

Query: 88  NRQF-LFQKDLEDKDNCRLTEKRYAGQSAGYSDGKFSCGSHLPEKFSVSEIALELPKRNK 147
           + +F ++ K+    ++ + T+K     + G      S  S   +K      A +  K+  
Sbjct: 264 SMEFDMWLKNYSQNNSKKPTKKDAKKDAKGKGSDAESVDSKDAKKDKKG--ATKDTKKGA 323

Query: 148 DEDVDSGFRNLSGEKLFACLPEKDDWMDK---TMAKVTQNFNEGKTKNKNKDSREAKYGD 207
            +D +S     SG+   A   +K+   DK          +   G +K+  KDS++ K   
Sbjct: 324 KKDTESTDAE-SGDSKDAKKGKKESKKDKKKDAKKDAASDAESGDSKDAKKDSKKGK--- 383

Query: 208 LGIQEESKFRKRGTIQPMVKDGVDSVEKNADAKTEEKKWKGNGR-DEKSKDKQKDKD--- 267
              ++  K  K+   +   +        + DAK + KK K + + D+K KD +KD +   
Sbjct: 384 ---KDSKKDNKKKDAKKDAESTDAESGDSKDAKKDSKKGKKDSKKDDKKKDAKKDAESTD 443

Query: 268 -------------KEGKGKDKERDKKKK--KKEKIEQKFGNLKKSKERYEGD 288
                        K+GK  DK++D KK     +   +  G+ KKSK+  + D
Sbjct: 444 AESGDSKNAKKDSKKGKKDDKKKDAKKDAVSTDADSESEGDAKKSKKDSKKD 486

BLAST of Cucsa.256650 vs. Swiss-Prot
Match: TOP1_CRIGR (DNA topoisomerase 1 OS=Cricetulus griseus GN=TOP1 PE=2 SV=1)

HSP 1 Score: 63.2 bits (152), Expect = 9.1e-09
Identity = 66/240 (27.50%), Postives = 96/240 (40.00%), Query Frame = 1

Query: 20  DLDLLKKDKHKKKRKKENRDHKEKKKSRSRHKRDKEKDRVKDKAKTLD----ESKLPDKI 79
           + D    D HK K K ++R+H+ K      HK+DKEKDR K K    +    E K  +K 
Sbjct: 13  EADFRLNDSHKHKDKHKDREHRHK-----EHKKDKEKDREKSKHSNSEHKDSEKKHKEKE 72

Query: 80  QCHNRQFLFQKDLEDKDNCRLTEKRYAGQSAGYSDGKFSCGSHLPEKFSVSEIALELPKR 139
           +  ++    +K  +DK   R  EKR   +     D K              E     P R
Sbjct: 73  KTKHKDGSSEKH-KDKHKDRDKEKRKEEKIRASGDAKIK---------KEKENGFSSPPR 132

Query: 140 NKDEDVDSGFRNLSGEKLFACLPEKDDWMDKTMAKVTQNFNEGKTKNKNKDSREAKYGDL 199
            KDE  D G+        FA  P K+D       +   + +    K K +D ++ K   L
Sbjct: 133 IKDEPDDDGY--------FA--PPKEDIKPLKRPRDEDDADYKPKKIKTEDIKKEKKRKL 192

Query: 200 GIQEESKFRKRGTIQPMVKDGVDSVEKNADAKTEEKKWKGNGRDEKSKDKQKDKDKEGKG 256
             +E+ K +K        K      +K    K EE+KWK    +E+  +  K K  E KG
Sbjct: 193 EEEEDGKLKKTKNKDKDKKGAESDNKKKKPKKEEEQKWKW-WEEERYPEGIKWKFLEHKG 226

HSP 2 Score: 40.4 bits (93), Expect = 6.3e-02
Identity = 29/85 (34.12%), Postives = 43/85 (50.59%), Query Frame = 1

Query: 214 KDGVDSVEKNADAKTEEKKWKGNGRDEKSKDKQKDKDK-------EGKGKDKERDKKKKK 273
           KD     EK+  + +E K       D + K K+K+K K       + K K K+RDK+K+K
Sbjct: 40  KDKEKDREKSKHSNSEHK-------DSEKKHKEKEKTKHKDGSSEKHKDKHKDRDKEKRK 99

Query: 274 KEKIEQKFGNLKKSKERYEGDVTSP 292
           +EKI    G+ K  KE+  G  + P
Sbjct: 100 EEKIRAS-GDAKIKKEKENGFSSPP 116

HSP 3 Score: 39.3 bits (90), Expect = 1.4e-01
Identity = 24/53 (45.28%), Postives = 32/53 (60.38%), Query Frame = 1

Query: 222 KNADAKTEEKKWKGNGRDEKSKDKQKDKDKEGKGKDKERDKKKKKKEKIEQKF 275
           K  D K E+K+      D K K K K+KDK+ KG + +  KKK KKE+ EQK+
Sbjct: 155 KTEDIKKEKKRKLEEEEDGKLK-KTKNKDKDKKGAESDNKKKKPKKEE-EQKW 205

HSP 4 Score: 39.3 bits (90), Expect = 1.4e-01
Identity = 42/115 (36.52%), Postives = 58/115 (50.43%), Query Frame = 1

Query: 179 KTKNKNKDSREAKYGDLGIQEESKFRKRGTIQPMVKDGVDSVEKNADAKTEEKKWKGNGR 238
           K K+K+KD RE ++     +E  K ++        KD   S   N++ K  EKK K    
Sbjct: 23  KHKDKHKD-REHRH-----KEHKKDKE--------KDREKSKHSNSEHKDSEKKHK---- 82

Query: 239 DEKSKDKQKDKDKEGKGKDK-ER-DKKKKKKEKIEQKFGNLKKSKERYEGDVTSP 292
            EK K K KD   E K KDK +  DK+K+K+EKI    G+ K  KE+  G  + P
Sbjct: 83  -EKEKTKHKDGSSE-KHKDKHKDRDKEKRKEEKIRAS-GDAKIKKEKENGFSSPP 116

BLAST of Cucsa.256650 vs. Swiss-Prot
Match: TAF3_HUMAN (Transcription initiation factor TFIID subunit 3 OS=Homo sapiens GN=TAF3 PE=1 SV=1)

HSP 1 Score: 62.0 bits (149), Expect = 2.0e-08
Identity = 75/307 (24.43%), Postives = 127/307 (41.37%), Query Frame = 1

Query: 19  EDLDLLKKDKHKKKRKKENRDHKEKKKSRSRHKRDKEKDRVKDKAKTLDESKLPDKIQCH 78
           E L  + ++K K     E +   +K+      K++K++DR ++K K  D+SK  DK++  
Sbjct: 503 EPLHKVYEEKTKLPSSVEVKKKLKKELKTKMKKKEKQRDREREKDKNKDKSKEKDKVKEK 562

Query: 79  NRQFLFQKDLEDKDNCRLTEKRYAGQSAGYSDGKFSCGSHLPEKFSVSEIALELPKRNKD 138
                 +KD E     +   K +  +               P KF + E           
Sbjct: 563 ------EKDKETGRETKYPWKEFLKEEEAD-----------PYKFKIKEF---------- 622

Query: 139 EDVDSGFRNLSGEKLFACLPEKDDWMDKTMAKVTQNFNEGKTKNKNKDSRE-AKYGDLGI 198
           EDVD   +   G        EK+   DK      ++  +GK   K+KD RE  K  D G 
Sbjct: 623 EDVDPKVKLKDG----LVRKEKEKHKDK-----KKDREKGK---KDKDKREKEKVKDKGR 682

Query: 199 QEESKFRKRGTIQP-------------------MVKDGVDSVEKNADAKTEEKKWKGNGR 258
           +++ K      + P                   M+   +  + +    + E+ K K   +
Sbjct: 683 EDKMKAPAPPLVLPPKELALPLFSPATASRVPAMLPSLLPVLPEKLFEEKEKVKEKEKKK 742

Query: 259 DEKSKDKQKDKDKEGKGKDKERDKKKKKKEKIEQKFGNLKKSKERYEGDVTSPDSIITSQ 306
           D+K K K+K+K+KE   K+KER+K+K+++EK E++    K  K + E    +P  +I   
Sbjct: 743 DKKEKKKKKEKEKE--KKEKEREKEKREREKREKEKEKHKHEKIKVEPVALAPSPVIPRL 768

HSP 2 Score: 35.4 bits (80), Expect = 2.0e+00
Identity = 16/44 (36.36%), Postives = 30/44 (68.18%), Query Frame = 1

Query: 13  EKKARIEDLDLLKKDKHKKKRKKENRDHKEKKKSRSRHKRDKEK 57
           EKK + E     +K+K +K+R+KE R+ ++++K + +HK +K K
Sbjct: 707 EKKKKKEK----EKEKKEKEREKEKREREKREKEKEKHKHEKIK 746

BLAST of Cucsa.256650 vs. TrEMBL
Match: A0A0A0L7W2_CUCSA (Uncharacterized protein OS=Cucumis sativus GN=Csa_3G129690 PE=4 SV=1)

HSP 1 Score: 899.4 bits (2323), Expect = 1.8e-258
Identity = 445/475 (93.68%), Postives = 445/475 (93.68%), Query Frame = 1

Query: 1   MSRCFPFPPPGYEKKARIEDLDLLKKDKHKKKRKKENRDHKEKKKSRSRHKRDKEKDRVK 60
           MSRCFPFPPPGYEKKARIEDLDLLKKDKHKKKRKKENRDHKEKKKSRSRHKRDKEKDRVK
Sbjct: 1   MSRCFPFPPPGYEKKARIEDLDLLKKDKHKKKRKKENRDHKEKKKSRSRHKRDKEKDRVK 60

Query: 61  DKAKTLDESKLPDKIQCHNRQFLFQKDLEDKDNCRLTEKRYAGQSAGYSDGKFSCGSHLP 120
           DKAKTLDESKLPDKIQCHNRQFLFQKDLEDKDNCRLTEKRYAGQSAGYSDGKFSCGSHLP
Sbjct: 61  DKAKTLDESKLPDKIQCHNRQFLFQKDLEDKDNCRLTEKRYAGQSAGYSDGKFSCGSHLP 120

Query: 121 EKFSVSEIALELPKRNKDEDVDSGFRNLSGEKLFACLPEKDDWMDKTMAKVTQNFNEGKT 180
           EKFSVSEIALELPKRNKDEDVDSGFRNLSGEKLFACLPEKDDWMDKTMAKVTQNFNEGKT
Sbjct: 121 EKFSVSEIALELPKRNKDEDVDSGFRNLSGEKLFACLPEKDDWMDKTMAKVTQNFNEGKT 180

Query: 181 KNKNKDSREAKYGDLGIQEESKFRKRGTIQPMVKDGVDSVEKNADAKTEEKKWKGNGRDE 240
           KNKNKDSREAKYGDLGIQEESKFRKRGTIQPMVKDGVDSVEKNADAKTEEKKWKGNGRDE
Sbjct: 181 KNKNKDSREAKYGDLGIQEESKFRKRGTIQPMVKDGVDSVEKNADAKTEEKKWKGNGRDE 240

Query: 241 KSKDKQKDKDKEGKGKDKERDKKKKKKEKIEQKFGNLKKSKERYEGDVTSPDSIITSQLL 300
           KSKDKQKDKDKEGKGKDKERDKKKKKKEKIEQKFGNLKKSKERYEGDVTSPDSIITSQLL
Sbjct: 241 KSKDKQKDKDKEGKGKDKERDKKKKKKEKIEQKFGNLKKSKERYEGDVTSPDSIITSQLL 300

Query: 301 RDSHGSVASIGSLMKRKEFENNGIFGESIGPFLLKLSRMALFYYLTDKFLLTFSTAKDHM 360
           RDSHGSVASIGSLMKRKEFENNGIF                              AKDHM
Sbjct: 301 RDSHGSVASIGSLMKRKEFENNGIF------------------------------AKDHM 360

Query: 361 SSNSTKFTSSSDLPRVNHKMLKSCQSSAPTASNWQVQSKPPHPDLECLSQVYLVPKMEEW 420
           SSNSTKFTSSSDLPRVNHKMLKSCQSSAPTASNWQVQSKPPHPDLECLSQVYLVPKMEEW
Sbjct: 361 SSNSTKFTSSSDLPRVNHKMLKSCQSSAPTASNWQVQSKPPHPDLECLSQVYLVPKMEEW 420

Query: 421 SDFCDQDWLYSSSASKLKKLNAHRYEMETTTPYVWAEAMQIDSIDICALPYVVPY 476
           SDFCDQDWLYSSSASKLKKLNAHRYEMETTTPYVWAEAMQIDSIDICALPYVVPY
Sbjct: 421 SDFCDQDWLYSSSASKLKKLNAHRYEMETTTPYVWAEAMQIDSIDICALPYVVPY 445

BLAST of Cucsa.256650 vs. TrEMBL
Match: D7SNQ8_VITVI (Putative uncharacterized protein OS=Vitis vinifera GN=VIT_05s0029g00470 PE=4 SV=1)

HSP 1 Score: 298.9 bits (764), Expect = 1.1e-77
Identity = 211/529 (39.89%), Postives = 284/529 (53.69%), Query Frame = 1

Query: 1   MSRCFPFPPPGYEKKARIEDLDLLKKDKHK-KKRKKENRD------------------HK 60
           MSRCFPFPPPGYEKKAR +D DLLKK+KH+ KK KKE R+                  HK
Sbjct: 1   MSRCFPFPPPGYEKKARTDDADLLKKEKHREKKHKKEKREKEKKEGKEKREKDRGDGRHK 60

Query: 61  EKKKSRSRH---KRDKEKDRVKDKAK--TLDESKLPDKIQCHNRQFLFQKDLEDKD-NCR 120
           EKK  + +H   K+DKEKDR K+K K  T DE +L  + +  N + L QK++ D+D N  
Sbjct: 61  EKKDRKDKHRDRKKDKEKDREKEKDKNITSDEKRLASEFEGFNAEKLSQKEVRDRDKNSV 120

Query: 121 LTEKRYAGQSAGYSDGKFSCGSHLPEKFSVSEIALELPKRNKDEDVDSGFRNLSGEKLFA 180
             EKR+AGQ  GY++ K +  SHL E    S+   EL +R + ED  +G  N   EK   
Sbjct: 121 SNEKRFAGQLVGYNEEKLTQSSHLGEGNKDSKFLQELGRRIRAEDGGNG--NQLVEKFTG 180

Query: 181 CLPEKDDWMDKTMAKVTQNFNEGKTKNKNKDSREAKYGDLGIQEESKFRKRGTIQPMVKD 240
               KD+ M + +AK      EGK KNK+K   + K    G+++ES+       Q +   
Sbjct: 181 TDRRKDEAMARLVAKDVGIRTEGKEKNKDKRGEDRKIDGQGVRDESRISGNSVAQSLTGT 240

Query: 241 GV-------DSVEKNADAKTEEK-KWKGNGRDEKSKDKQKDKDKEGK--GKDKERDKKKK 300
           G+         +EK  + + E K K K    D+K  DK+KD+D+E K  GK+K+RDK+K+
Sbjct: 241 GLHRLEGTPKPLEKKIERRMEAKEKTKDKEGDDKRGDKRKDRDREKKSQGKEKDRDKEKR 300

Query: 301 K----KEKIEQKFGNLKKSKERYEGDVTSPDSIITSQLLRDSHGSVASIGSLMKRKEFEN 360
           K    KEKIE K   L KSKE  + DVT   +I TS L +DS+ S A+ G+  KRK+ + 
Sbjct: 301 KEEKEKEKIEHKNKELDKSKESNKDDVTVTHNIKTSHLHKDSNKSAATEGNHRKRKDSDT 360

Query: 361 NG-IFGESIGPFLLKLSRMALFYYLTD--------KFLLTFSTAKDHMSSNSTKFTSSSD 420
           NG +    + P   KL R      + +          +L  S  +   ++N         
Sbjct: 361 NGFLHVNEVKP--NKLPRPTSHPLIENGRKLEPCQTSILITSDRQGGTANNLKVENKEHK 420

Query: 421 LPRVNHKMLKSCQSSAPTASNWQ------VQSKPPHPDLECLSQVYLVPKMEEWSDFCDQ 476
           L  +      S  S+ P +   Q         KPPHPD + LSQV LVPKMEEWSDF DQ
Sbjct: 421 LNGIIGAQPFSVASTKPLSVASQADQIADASKKPPHPDSKYLSQVLLVPKMEEWSDFDDQ 480

BLAST of Cucsa.256650 vs. TrEMBL
Match: A0A061EJK1_THECC (Electron transport complex protein rnfC, putative isoform 1 OS=Theobroma cacao GN=TCM_020238 PE=4 SV=1)

HSP 1 Score: 275.0 bits (702), Expect = 1.7e-70
Identity = 198/545 (36.33%), Postives = 284/545 (52.11%), Query Frame = 1

Query: 1   MSRCFPFPPPGYEKKARIEDLDLLKKDKHKKKR---------KKENRD----------HK 60
           MSRCFPFPPPGYEKKAR +D+D++KK+K K+K+         K+EN++          HK
Sbjct: 1   MSRCFPFPPPGYEKKARTDDVDIIKKEKQKEKKHKKDKKEKEKRENKEKKEKDRSDGKHK 60

Query: 61  EKKKSRSRHK-RDKEKDRVKDK--------------------AKTLDESKLPDKIQCHNR 120
           +KK  + +HK + KEKDR K+K                     K  DE KL  K + H+ 
Sbjct: 61  DKKDKKEKHKDKKKEKDRDKEKDRSNNSEEKKFPGHPEGQNGEKISDEKKLQGKFEGHSG 120

Query: 121 QFLFQKDL-EDKDNCRLT-EKRYAGQSAGYSDGKFSCGSHLPEKFSVSEIALELPKRNKD 180
           +   QK+   DKD    + EK+ AGQ +GY+  K +  SHL E F  S+   EL +R +D
Sbjct: 121 EKFIQKEKGRDKDRSSFSGEKKLAGQFSGYNGEKITQNSHLAEDFRDSKFVQELGRRVRD 180

Query: 181 EDVDSGFRNLSGEKLFACLPEKDDWMDKTMAKVTQNFNEGKTKNKNKDSREAKYGDLGIQ 240
           E   +G  N   ++      ++D+ M + +AK T N  E K K+K  D R  K    GI+
Sbjct: 181 EGAGTG--NQLADRFMGTDQKRDEGMVRLVAK-TANILEEKEKSKRSDDR--KLDVQGIR 240

Query: 241 EESKFRKRGTIQPM---VKDGVDSVEKNADAKTEEK-----KWKGNGRDEKSKDKQKDKD 300
           EE++      +Q +    K  V+ + +  +  TE +     K K    D+K +DK+KDKD
Sbjct: 241 EETRSSGNAMVQNLPGAAKARVEGIPRQVEKDTERRMEGKEKTKEKQSDDKIRDKRKDKD 300

Query: 301 KEGK--GKDKERDKKKKKKEKIEQKFGNLKKSKERYEG----DVTSPDSIITSQLLRDSH 360
           +E K  GKDK+RDK+KKK+EK + K       ++  +G    D     ++ TS   ++ +
Sbjct: 301 REKKSHGKDKDRDKEKKKEEKAKAKSERRNIEQDNVKGSNKDDPVGTINLKTSHPSKEGN 360

Query: 361 GSVASIGSLMKRKEFENNGIFGESIGPFLLKLSRMALFYYLTDKF-LLTFSTAKDHMSSN 420
               + G+L KRK+ E NG F  +           +  + LTD    L    A   ++S+
Sbjct: 361 KDAVAEGNLRKRKDLEKNGFFHVNDNKPNKFPRTTSSSHPLTDNGRTLESCQAPIPLTSD 420

Query: 421 STKFTSSSDLPRVNHKM-------LKSCQSSAPTASNWQ------VQSKPPHPDLECLSQ 476
           S    +S  +   + K+       L S   + P A+N Q      V  KPPHPD + LSQ
Sbjct: 421 SQGAGTSLKVDNKDCKVNGIIEAQLLSVSPTKPLAANAQAIQIDEVSMKPPHPDSKYLSQ 480

BLAST of Cucsa.256650 vs. TrEMBL
Match: A0A067KWI7_JATCU (Uncharacterized protein OS=Jatropha curcas GN=JCGZ_01090 PE=4 SV=1)

HSP 1 Score: 251.1 bits (640), Expect = 2.6e-63
Identity = 195/556 (35.07%), Postives = 290/556 (52.16%), Query Frame = 1

Query: 1   MSRCFPFPPPGYEKKARIEDLDLLKKDKHKKKR---------KKENRDHKEKKKSRSRHK 60
           MSRCFPFPPPGYEKK R +D+DLLKK+K+++K+         KKE +D ++K+K RS  K
Sbjct: 1   MSRCFPFPPPGYEKKTRTDDVDLLKKEKNREKKQKKEKKNREKKEGKDREKKEKDRSDGK 60

Query: 61  -----------RDKEKDRVKDKAKTL--------------------DESKLPDKIQCHNR 120
                      RDK++DR  DK K +                    DE +LP K + ++ 
Sbjct: 61  HRDKKEKKEKQRDKKEDRDTDKDKIIASNEKRLPGQSESNNGDKVSDEKRLPAKFEINSG 120

Query: 121 QFLFQKDLEDKDNCRLT---EKRYAGQSAGYSDGKFSCGSHLPEKFSVSEIALELPKRNK 180
           +   QK    +D  R +   EK++AGQ +GY++ + S  +    +    +   E+ KR +
Sbjct: 121 EIFIQKG-NGRDLGRSSISGEKKFAGQFSGYNE-RVSQNARPSVQPKELKFVQEVDKRIR 180

Query: 181 DEDVDSGFRNLSGEKLFACLPEKDDWMDKTMAKVTQNFNEGKTKNKNKDSREAKYGDLGI 240
           DE   +G + +  EK       KD+ M + +AK T    + K KNK  D R  K G  GI
Sbjct: 181 DEAKGTGKQMV--EKFTGADARKDEGMFRLVAKATGTSVDVKEKNKIGDDR--KLGAQGI 240

Query: 241 QEESKFRKR-------GTIQPMVKDGVDSVEKNADAKTEEK-KWKGNGRDEKSKDKQKDK 300
           +E S+F          G +QP +     S+E + + + E K K +    D++  DK++DK
Sbjct: 241 REVSRFTGNAMAQSLPGMVQPRIDKTPRSLENDVEKRVEGKEKSRQKEGDDRHGDKRRDK 300

Query: 301 --DKEGKGKDKERDKKKKKKEKIEQK--FGNL--KKSKERYEGDVTSPDSIITSQLLRDS 360
             +K+G GKDK RDK++KK+EK++ K  + N+   K KE  +   T   +  TS L R+S
Sbjct: 301 IKEKDGLGKDKNRDKERKKEEKLKGKSEYKNMGPDKLKESNKDGPTGIHNAKTSHLPRES 360

Query: 361 HGSVASIGSLMKRKEFENNG-IFGESIGPFLLKLSRMALFYYLTDKFLL--TFSTAKDHM 420
             S  + G+L KRK+ + NG I    I P   K+SR+   +  T+   +  T  T+   +
Sbjct: 361 --SAVTEGNLKKRKDLDTNGFISANDIRP--NKVSRLTPSHPSTENGRISGTLQTSSPLI 420

Query: 421 SS-----NSTKFTSSSDLPR------------VNHKMLKSCQSSAPTASNWQVQS----K 476
           S      N+ K   + +L +                +  S   S PT +    ++    +
Sbjct: 421 SGRQGPLNNLK-ADAKELKKNGIIEPQALSVSTTQPLPLSTTKSLPTTTQADKKAEPPKR 480

BLAST of Cucsa.256650 vs. TrEMBL
Match: A0A0D2VMR4_GOSRA (Uncharacterized protein OS=Gossypium raimondii GN=B456_011G153200 PE=4 SV=1)

HSP 1 Score: 246.5 bits (628), Expect = 6.5e-62
Identity = 186/557 (33.39%), Postives = 282/557 (50.63%), Query Frame = 1

Query: 1   MSRCFPFPPPGYEKKARIEDLDLLKKDKHK-KKRKKENRD------------------HK 60
           MSRCFPFPPPGYEKKAR +D D LKK+K K KK KKE ++                  HK
Sbjct: 1   MSRCFPFPPPGYEKKARTDDADFLKKEKQKEKKHKKEKKEKERRENKEKKEKDKSDGKHK 60

Query: 61  EKKKSRSRHK-----RDKEKDRVKDKAKTLDESKLPDKIQCHN----------------- 120
           +KK  + +HK     ++K++D+ KD++  L+E K P + +  N                 
Sbjct: 61  DKKDKKEKHKDRDKKKEKDRDKEKDRSNNLEEKKFPGRPEGQNGEKTTVEKQLPGKSEGH 120

Query: 121 --RQFLFQKDLEDKD-NCRLTEKRYAGQSAGYSDGKFSCGSHLPEKFSVSEIALELPKRN 180
              +F+ ++   +KD N    EK++AGQ +GY+  K S  SH  E F  S+   EL +R 
Sbjct: 121 SGEKFIPKEKGSEKDRNSFSGEKKFAGQFSGYNGEKLS-QSHQAEDFRDSKFVHELGRRV 180

Query: 181 KDEDVDSGFRNLSGEKLFACLPEKDDWMDKTMAKVTQNFNEGKTKNKNKDSREAKYGDLG 240
           + E   +G  N   EK    +P++D+ M + +AK      E K KNK  D  ++K    G
Sbjct: 181 RGEGAGAG--NQLEEKHMGTVPKRDEGMVRLVAKTANALVEEKEKNKRSD--DSKSNMQG 240

Query: 241 IQEESKFRKRGTIQPMVK------DGVDSVEKNADAKTEE--KKWKGNGRDEKSKDKQKD 300
           I++E++      +Q +V       +G+     +++ + +E  +K K    D+K +DK K 
Sbjct: 241 IRDETRSGGNAMVQNIVGAVNARVEGIPIQVGSSNERRDEGKEKTKEKKSDDKIRDKHKT 300

Query: 301 KDKEGK--GKDKERDKKKKKKEKIEQK--------------FGNLKKSKERYEGDVTSPD 360
           KD E K  GKDK+RDK+KKKK+K ++K                NLK++ +       +P 
Sbjct: 301 KDGEKKSHGKDKDRDKEKKKKKKEKKKEEKAKAKGEHRNLELDNLKRNNKDDPVGTINPK 360

Query: 361 SIITSQLLRDSHGSVASIGSLMKRKEFENNGIFG-ESIGPFLLKLSRMALFYYLTDKFLL 420
           +  +S+    + G+V       KRK++ENNG      + P   KL R +    + +   L
Sbjct: 361 ASDSSK--EGNKGAVVEENH-RKRKDWENNGFLHVNDLKPN--KLPRTSSSVLMDNGKTL 420

Query: 421 TFSTAKDHMSSNSTKFTSSSDLPRVNHKMLKSCQS-------------SAPTASNWQVQS 476
               A   ++SNS    +   +    HK+  + ++             SA  +   +V  
Sbjct: 421 ESCQAPIPLASNSHGAGTGLKVNAKEHKLNGTSEAQVLSVSPATHLSASAQASQMDEVCK 480

BLAST of Cucsa.256650 vs. TAIR10
Match: AT5G48610.1 (AT5G48610.1 unknown protein)

HSP 1 Score: 108.6 bits (270), Expect = 1.1e-23
Identity = 96/337 (28.49%), Postives = 162/337 (48.07%), Query Frame = 1

Query: 1   MSRCFPFPPPGYEKKARIEDLDLLKKDKHKKKRKKENRDHKEKKKSRSRHK-RDKEKDRV 60
           MSRCFPFPPPGYEKK R ++ D L KDK+K+K+ K++++ +E K+ +S+ + +DK+K+R 
Sbjct: 1   MSRCFPFPPPGYEKKIRTDEADPLVKDKYKEKKHKKDKEKREGKEKKSKDRSKDKQKERK 60

Query: 61  KDKAKTLDESKLPDKIQCHNR-QFLFQKDLEDKDNCRLTEKRYAGQSAGYSDGKFSCGSH 120
           + K K  D+    DK +   + + L +K  E   N    E R        S+G+      
Sbjct: 61  EKKDKHKDQK---DKEKGKEKGKPLEEKKAELLTNAGHRENRVTDTVQNNSNGESKYVQD 120

Query: 121 LPEKFSVSEIALELPKRNKDEDVDSGFRNLSGEKLFACLPEKDDWMDKTMAKVTQNFNEG 180
           L  +    E A       + +  +     ++ EK F   P     +++T  +V  N    
Sbjct: 121 LARRIRYDEEATGSQSAQRIDHPNQKNVGIT-EKAFENSP-----IEETSHRVDDN---- 180

Query: 181 KTKNKNKDSREAKYGDLGIQEES--KFRKRGTIQPMVKDGVDSVEKNADAKTEEKKWKGN 240
           K  N  K+   AK  +  +   S     KR  +     +  D V +   A+   +K +  
Sbjct: 181 KRINNQKNFTAAKSSENAVSRVSFGADHKRAEVMGKPMENRDQVRQTESAEKSHRK-ENV 240

Query: 241 GRDEKSKDKQKDKDKEGKGKDKERDKKKKKKE---KIEQKFGNLKKSKERYEGDVTSPD- 300
            + EK +D++  K  E K KD+ ++KK++K E   K  Q+   L +  +  E +  SPD 
Sbjct: 241 TKSEKPRDQEGVKKTEAKDKDRNKEKKEEKTESINKTRQEKPKLIRGPKLEEREKDSPDL 300

Query: 301 -SIITSQLLRDSHGSVASIGSLMKRKEFENNG-IFGE 328
            +     + R S  ++ + G+L KRK+   NG ++GE
Sbjct: 301 RNCKLPDVSRTSIKNLHTEGNLGKRKDHMTNGFLYGE 323

BLAST of Cucsa.256650 vs. TAIR10
Match: AT1G56660.1 (AT1G56660.1 unknown protein)

HSP 1 Score: 76.3 bits (186), Expect = 5.9e-14
Identity = 81/332 (24.40%), Postives = 138/332 (41.57%), Query Frame = 1

Query: 25  KKDKHKKKRKKENRDHKEKKKSRSRHKRD-------------------KEKDRVKDKAKT 84
           KK+K +K+  K N D K K K     K D                   KEKD  K+K K 
Sbjct: 195 KKEKKQKEESKSNEDKKVKGKKEKGEKGDLEKEDEEKKKEHDETDQEMKEKDSKKNKKKE 254

Query: 85  LDESKLPDKIQCHNRQFLFQKDLEDKDNCRLTEKRYAGQSAGYSDGKFSCGSHLPEKFSV 144
            DES   +K +  +++   + +  +K++ +L  K+  G+     D       H   +  +
Sbjct: 255 KDESCAEEKKKKPDKEKKEKDESTEKEDKKLKGKKGKGEKPEKEDEGKKTKEHDATEQEM 314

Query: 145 SEIALELPKRNKDEDVDSGFRNLSGEKLFACLPEKDDWMDKTMAKVTQNFN--EGKTKNK 204
            + A +  +  K ++ D                +K+  +D+   K T++ +  EG+TK K
Sbjct: 315 DDEAADHKEGKKKKNKDKA-------------KKKETVIDEVCEKETKDKDDDEGETKQK 374

Query: 205 NKDSREAKY--GDLGIQEESKFRKRGTIQPMVKD---GVDSVEKNADAKTEEKKWKG--- 264
               +E K   G+  ++E+ K       + M +D        EK  +  TEEKK      
Sbjct: 375 KNKKKEKKSEKGEKDVKEDKKKENPLETEVMSRDIKLEEPEAEKKEEDDTEEKKKSKVEG 434

Query: 265 ----NGRDEKSKDKQKDKDKEGKGKDKERDKKKKKKEKIEQKFGNLKKSKERYEGDVTSP 322
                G+ +K KDK+K+K K+ K      D+++KK +  + K    K  +E+ + DV   
Sbjct: 435 GESEEGKKKKKKDKKKNKKKDTKEPKMTEDEEEKKDDSKDVKIEGSKAKEEKKDKDVKKK 494

HSP 2 Score: 70.9 bits (172), Expect = 2.5e-12
Identity = 75/286 (26.22%), Postives = 122/286 (42.66%), Query Frame = 1

Query: 14  KKARIEDLDLLKKDKHKKKRKKENRDHKEKKKSRSRHKRDKEKDRVKDKAKTLDESKLPD 73
           K+ + E+L+  K+ K KKK KKE  +   ++K++   K  K +D  ++K +  +E    +
Sbjct: 119 KEKKHEELEEEKEGK-KKKNKKEKDESGPEEKNKKADKEKKHEDVSQEKEELEEEDGKKN 178

Query: 74  KIQCHNRQFLFQKDLEDKDNCRLTEKRYAGQSAGYSDGKFSCGSHLPEKFSVSEIALELP 133
           K     ++   +   E+K      EK+   +S    D K        EK  + +   E  
Sbjct: 179 K-----KKEKDESGTEEKKKKPKKEKKQKEESKSNEDKKVKGKKEKGEKGDLEKEDEEKK 238

Query: 134 KRNKDEDVD----SGFRNLSGEKLFACLPEKDDWMDKTMAKVTQNFNEGKTKNKNKDSRE 193
           K + + D +       +N   EK  +C  EK    DK   +  ++  +   K K K  + 
Sbjct: 239 KEHDETDQEMKEKDSKKNKKKEKDESCAEEKKKKPDKEKKEKDESTEKEDKKLKGKKGKG 298

Query: 194 AKYGDLGIQEESKFRKR--GTIQPMVKDGVDSVEKNADAKTEEKKWKGNGRDEKSKDKQK 253
            K      ++E K  K    T Q M  +  D  E       ++ K K    DE  + + K
Sbjct: 299 EKPEK---EDEGKKTKEHDATEQEMDDEAADHKEGKKKKNKDKAKKKETVIDEVCEKETK 358

Query: 254 DKD-KEGKGKDKERDKKKKKKEKIEQKFGNLKKSKERYEGDVTSPD 293
           DKD  EG+ K K+  KK+KK EK E+     KK +   E +V S D
Sbjct: 359 DKDDDEGETKQKKNKKKEKKSEKGEKDVKEDKKKENPLETEVMSRD 395

HSP 3 Score: 53.5 bits (127), Expect = 4.1e-07
Identity = 58/273 (21.25%), Postives = 115/273 (42.12%), Query Frame = 1

Query: 13  EKKARIEDLDLLKKDKHKKKRKKENRDHKEKKKSRSRHKRDKEKDRVKDKAKTLDESKLP 72
           E KA+  +    KKD+    + K++++ K+ K   S  K DK+ D+ KD  K + +    
Sbjct: 36  EVKAKSIEKVKAKKDEESSGKSKKDKEKKKGKNVDSEVKEDKDDDKKKD-GKMVSKKHEE 95

Query: 73  DKIQCHNRQFLFQKDLEDKDNCRLTEKRYAGQSAGYSDGKFSCGSHLPEKFSVSEIALEL 132
                  ++   + +  +K++ +  EK++  +     +GK        ++    E   + 
Sbjct: 96  GHGDLEVKESDVKVEEHEKEHKKGKEKKHE-ELEEEKEGKKKKNKKEKDESGPEEKNKKA 155

Query: 133 PKRNKDEDVDSGFRNLSGEKLFACLPEKDDWMDKTMAKVTQNFNEGKTKNKNKDSREAKY 192
            K  K EDV      L          E++D       +  ++  E K K   K+ ++ + 
Sbjct: 156 DKEKKHEDVSQEKEEL----------EEEDGKKNKKKEKDESGTEEKKKKPKKEKKQKEE 215

Query: 193 GDLGIQEESKFRKRGTIQPMVKDGVDSVEKNADAKTEEKKWKGNGRDEKSKDKQKDKDKE 252
                 ++ K +K    +  ++   +  +K  D   +E K K +   +K+K K+KD+   
Sbjct: 216 SKSNEDKKVKGKKEKGEKGDLEKEDEEKKKEHDETDQEMKEKDS---KKNKKKEKDESCA 275

Query: 253 GKGKDKERDKKKKKKEKIEQKFGNLKKSKERYE 286
            + K K   +KK+K E  E++   LK  K + E
Sbjct: 276 EEKKKKPDKEKKEKDESTEKEDKKLKGKKGKGE 293

HSP 4 Score: 48.1 bits (113), Expect = 1.7e-05
Identity = 63/264 (23.86%), Postives = 107/264 (40.53%), Query Frame = 1

Query: 25  KKDKHKKKRKKENRDHKEKKKSRSRHKRDKEKDRVKDKAKTLDESKLPDKIQCHNRQFLF 84
           K++K   K K +  D KEK ++       K K   K KAK  +ES    K          
Sbjct: 9   KEEKLHVKIKTQELDPKEKGENVEVEMEVKAKSIEKVKAKKDEESSGKSK---------- 68

Query: 85  QKDLEDKDNCRLTEKRYAGQSAGYSDGKFSCGSHLPEKFSVSEIALELPKRNKDEDVDSG 144
            KD E K    +  +    +     D K   G  + +K       LE+ + +   +    
Sbjct: 69  -KDKEKKKGKNVDSEVKEDKD----DDKKKDGKMVSKKHEEGHGDLEVKESDVKVEEHEK 128

Query: 145 FRNLSGEKLFACLPEKDDWMDKTMAKVTQNFNEGKTKNKNKDSREAKYGDLGIQEESKFR 204
                 EK    L E+ +   K   K  +    G  +   K  +E K+ D+  QE+ +  
Sbjct: 129 EHKKGKEKKHEELEEEKEGKKKKNKK--EKDESGPEEKNKKADKEKKHEDVS-QEKEELE 188

Query: 205 KRGTIQPMVKDGVDSVEKNADAK-TEEKKWKGNGRDEKSKDKQKDKDKEGKGKDKERDKK 264
           +        +DG  + +K  D   TEEKK K     ++ ++ + ++DK+ KGK ++ +K 
Sbjct: 189 E--------EDGKKNKKKEKDESGTEEKKKKPKKEKKQKEESKSNEDKKVKGKKEKGEKG 246

Query: 265 KKKKEKIEQKFGNLKKSKERYEGD 288
             +KE  E+K  + +  +E  E D
Sbjct: 249 DLEKEDEEKKKEHDETDQEMKEKD 246

HSP 5 Score: 33.1 bits (74), Expect = 5.7e-01
Identity = 21/64 (32.81%), Postives = 35/64 (54.69%), Query Frame = 1

Query: 13  EKKARIE--DLDLLKKDKHKKKRKKENRDHKEKKKSR-SRHKRDKEKDRVKDKAKTLDES 72
           +KK+++E  + +  KK K K K+K + +D KE K +     K+D  KD   + +K  +E 
Sbjct: 414 KKKSKVEGGESEEGKKKKKKDKKKNKKKDTKEPKMTEDEEEKKDDSKDVKIEGSKAKEEK 473

Query: 73  KLPD 74
           K  D
Sbjct: 474 KDKD 477

BLAST of Cucsa.256650 vs. TAIR10
Match: AT4G35940.1 (AT4G35940.1 unknown protein)

HSP 1 Score: 73.9 bits (180), Expect = 2.9e-13
Identity = 70/291 (24.05%), Postives = 127/291 (43.64%), Query Frame = 1

Query: 1   MSRCFPFPPPGY------EKKARIEDLDLLKKDKHKKKRKKENRDHKEKKKSRSRHKRDK 60
           MSRCFPFPPPGY      ++   +  +  +++   K++R+K+ R  K+ KK +   K  K
Sbjct: 1   MSRCFPFPPPGYVLNGIRDEAVIVSSIKGVEEKAKKEQRRKDRRSDKKDKKDKKERKEKK 60

Query: 61  EKDRVKDKAKTLDESKLPDKIQCHNRQ--------FLFQKDLEDKDNCRLTEKRYAGQSA 120
           EK   K K +  +  ++  + + H R+         LF K  E + NC         +  
Sbjct: 61  EKKEKKRKER--EGKEVGSEKRSHKRRRKEDGAKVDLFHKLKESEVNCLEKSSLTVEREL 120

Query: 121 GYSDGKFSCGSHLPEKFSVSEIALELPKRNK-DEDVDSGFRNLSGEKLFACLPEKDDWMD 180
             S  + SC S L    + +E+   LPK+ +  + +D    N + EK      EK   +D
Sbjct: 121 LQSTSQNSCDSTL----NSNEM---LPKQKEVQQPLDGRHNNNNNEKRV----EKQQPLD 180

Query: 181 KTMAKVTQNFNEGKTKNKNKDSREAKYGDLGIQEESKFRKRGTIQPMVKDGVDSVEKNAD 240
                       G+  N N+   E +    G    +  ++    QP+     +    N +
Sbjct: 181 ------------GRHNNNNEKRVEKQQPLDGRHNNNNEKRIEKQQPL-----NGRHNNNN 240

Query: 241 AKTEEKKWKGNGRDEKSKDKQKDKDKEGKGKDKERDKKKKKKEKIEQKFGN 277
            K  EK+   NGR   + +K+ +K +   G+   ++K+K+K++ ++ +  N
Sbjct: 241 EKLMEKQQPLNGRHNNNNEKRIEKQQPLNGRHNNKEKQKEKQQPLDVRHNN 261

HSP 2 Score: 40.0 bits (92), Expect = 4.7e-03
Identity = 21/55 (38.18%), Postives = 36/55 (65.45%), Query Frame = 1

Query: 229 EEKKWKGNGRDEKSKDKQKDKDKEGKGKDKERDKKKKKKEKIEQKFGNLKKSKER 284
           EEK  K   R ++  DK+  KDK+ + K+K+  K+KK+KE+  ++ G+ K+S +R
Sbjct: 31  EEKAKKEQRRKDRRSDKKDKKDKKER-KEKKEKKEKKRKEREGKEVGSEKRSHKR 84

HSP 3 Score: 35.0 bits (79), Expect = 1.5e-01
Identity = 30/130 (23.08%), Postives = 63/130 (48.46%), Query Frame = 1

Query: 196 GIQEESKFRKRGTIQPMVKDGVDSVEKNADAKTEEKKWKGNGRDEKSKDKQKDKDKEGK- 255
           G++E++K  +R               K+  +  ++KK K   +++K K ++K K++EGK 
Sbjct: 29  GVEEKAKKEQR--------------RKDRRSDKKDKKDKKERKEKKEKKEKKRKEREGKE 88

Query: 256 -GKDKERDKKKKKKEKIE-QKFGNLKKSKER--YEGDVTSPDSIITSQLLRDSHGSVASI 315
            G +K   K+++K++  +   F  LK+S+     +  +T    ++ S        ++ S 
Sbjct: 89  VGSEKRSHKRRRKEDGAKVDLFHKLKESEVNCLEKSSLTVERELLQSTSQNSCDSTLNSN 144

Query: 316 GSLMKRKEFE 321
             L K+KE +
Sbjct: 149 EMLPKQKEVQ 144

BLAST of Cucsa.256650 vs. TAIR10
Match: AT5G43490.1 (AT5G43490.1 unknown protein)

HSP 1 Score: 65.1 bits (157), Expect = 1.4e-10
Identity = 42/108 (38.89%), Postives = 57/108 (52.78%), Query Frame = 1

Query: 376 VNHKMLKSCQSSAPTAS-------NWQVQSKPPHPDLECLSQVYLVPKMEEWSDFCD-QD 435
           V+ K  K C  S   A+       N +  +KPPHPDL+ L+Q+  VP  E   +  D Q+
Sbjct: 79  VSGKKHKVCPPSPLAATMKVKVKENGEPSAKPPHPDLKYLNQILHVPTRELLPEIDDDQE 138

Query: 436 WLYSSSASKLKKLNAHRYEMETTTPYVWAEAMQIDSIDICALPYVVPY 476
           WL   S  KLKK      +   +   VW +A +I+S DI ALPYVVP+
Sbjct: 139 WLLGQSGIKLKKARTDPPDSGESLQ-VWNQAFRIESADITALPYVVPF 185

BLAST of Cucsa.256650 vs. TAIR10
Match: AT3G28770.1 (AT3G28770.1 Protein of unknown function (DUF1216))

HSP 1 Score: 65.1 bits (157), Expect = 1.4e-10
Identity = 71/283 (25.09%), Postives = 127/283 (44.88%), Query Frame = 1

Query: 13   EKKARIEDLDLLKKDKHKK--------KRKKENRDHKEKKKSRSRHKRDKEKDRVKD-KA 72
            E K    + +L K++ +KK        K K+EN+D+KEKK+S     +++EK   ++ K+
Sbjct: 961  EDKKEYVNNELKKQEDNKKETTKSENSKLKEENKDNKEKKESEDSASKNREKKEYEEKKS 1020

Query: 73   KTLDESKLPDKIQCHNRQFLFQKDLEDKDNCRLTEKRYAGQSAGYSDGKFSCGSHLPEKF 132
            KT +E+K   K           K  E+KD+     K+   +S    D K        ++ 
Sbjct: 1021 KTKEEAKKEKKKS-------QDKKREEKDSEERKSKKEKEES---RDLKAKKKEEETKEK 1080

Query: 133  SVSEIALELPKRNKDEDVDSGFRNLSGEKLFACLPEKDDWMDKTMAKVTQNFNEGKTKNK 192
              SE      K +K E  D+       +K      E+     K   K      E +  NK
Sbjct: 1081 KESENHKSKKKEDKKEHEDNKSMKKEEDKKEKKKHEESKSRKKEEDKKDMEKLEDQNSNK 1140

Query: 193  NKDSREAKYGDLGIQEESKFRKRGTIQPMVKDGVDSVEKNADAKTEEKKWKGNGRDEKSK 252
             K+ +  K       ++S+  K    +   K+  ++ EK+   + E  K + N  D+K K
Sbjct: 1141 KKEDKNEK-------KKSQHVKLVKKESDKKEKKENEEKSETKEIESSKSQKNEVDKKEK 1200

Query: 253  DKQKDKDKEGKGKDKERDKKK-KKKEKIEQKFGNLKKSKERYE 286
               KD+ K+ + + KE ++KK KK E+  +K  +++++K++ E
Sbjct: 1201 KSSKDQQKKKEKEMKESEEKKLKKNEEDRKKQTSVEENKKQKE 1226

HSP 2 Score: 63.9 bits (154), Expect = 3.0e-10
Identity = 64/275 (23.27%), Postives = 120/275 (43.64%), Query Frame = 1

Query: 13   EKKARIEDLDLLKKDKHKKK---RKKENRDHKEKKKSRSRHKRDKEKDRVKDKAKTLDES 72
            + K + E  D   K++ KK+   +K + ++  +K+K +S+ K+ +EKD  + K+K   E 
Sbjct: 995  DNKEKKESEDSASKNREKKEYEEKKSKTKEEAKKEKKKSQDKKREEKDSEERKSKKEKEE 1054

Query: 73   KLPDKIQCHNRQFLFQKDLEDKDNCRLTEKRYAGQSAGYSDGKFSCGSHLPEKFSVSEIA 132
                K +    +   +K+ E+  + +  +K+       + D K    S   E+    +  
Sbjct: 1055 SRDLKAKKKEEETKEKKESENHKSKKKEDKK------EHEDNK----SMKKEEDKKEKKK 1114

Query: 133  LELPKRNKDEDVDSGFRNLSGEKLFACLPEKDDWMDKTMAKVTQNFNEGKTKNKNKDSRE 192
             E  K  K E+       L  +       +K++       K+ +  ++ K K +N++  E
Sbjct: 1115 HEESKSRKKEEDKKDMEKLEDQNSNKKKEDKNEKKKSQHVKLVKKESDKKEKKENEEKSE 1174

Query: 193  AKYGDLGIQEESKFRKRGTIQPMVKDGVDSVEKNAD--AKTEEKKWKGNGRDEKSKDKQK 252
             K       E SK +K    +   K   D  +K      ++EEKK K N  D K K    
Sbjct: 1175 TKE-----IESSKSQKNEVDKKEKKSSKDQQKKKEKEMKESEEKKLKKNEEDRK-KQTSV 1234

Query: 253  DKDKEGKGKDKERDKKKKKKEKIEQKFGNLKKSKE 283
            +++K+ K   KE++K K  K+   ++ G  K+S E
Sbjct: 1235 EENKKQKETKKEKNKPKDDKKNTTKQSGGKKESME 1253

HSP 3 Score: 63.2 bits (152), Expect = 5.1e-10
Identity = 68/277 (24.55%), Postives = 117/277 (42.24%), Query Frame = 1

Query: 25   KKDKHKKKRKKENRDHK-----EKKKSRSRHKRDKEKDRV-KDKAKTLDESKLPDKIQCH 84
            + +K +K  KKE++D K     + KK  S   RD+ K+R  +D  +  +ESK    ++  
Sbjct: 781  ESEKVEKGEKKESKDAKSVETKDNKKLSSTENRDEAKERSGEDNKEDKEESKDYQSVEAK 840

Query: 85   NRQFLFQKDLE---DKDNCRLTEKRYAGQSAGYSDGKFSCGSHLPEKFSVSEIALELPKR 144
             +      D      +D+  L + R     A   +        +      S   +     
Sbjct: 841  EKNENGGVDTNVGNKEDSKDLKDDRSVEVKANKEESMKKKREEVQRNDKSSTKEVRDFAN 900

Query: 145  NKDEDVDSGFRNLSGEKLFACLPEKDDWMDKTMAKVTQNFNEGKTKNKNKDSREAKYGDL 204
            N D DV  G    SGE +     EK +   +         ++ K K+K K  +E+K  ++
Sbjct: 901  NMDIDVQKG----SGESVKYKKDEKKEGNKEENKDTINTSSKQKGKDKKKKKKESKNSNM 960

Query: 205  GIQEESKFRKRGTIQPMVKDGVDSVEKNADAKTEEK----KWKGNGRDEKSKDKQKDKDK 264
              +EE K           +D      K+ ++K +E+    K K    D  SK+++K + +
Sbjct: 961  KKKEEDKKEYVNNELKKQEDNKKETTKSENSKLKEENKDNKEKKESEDSASKNREKKEYE 1020

Query: 265  EGKGKDKE---RDKKKKKKEKIEQKFGNLKKSKERYE 286
            E K K KE   ++KKK + +K E+K    +KSK+  E
Sbjct: 1021 EKKSKTKEEAKKEKKKSQDKKREEKDSEERKSKKEKE 1053

HSP 4 Score: 60.8 bits (146), Expect = 2.5e-09
Identity = 68/291 (23.37%), Postives = 123/291 (42.27%), Query Frame = 1

Query: 30   KKKRKKENRDHKEKKKSRSRHKRDKEKDRVKDKAKTLDESKLPDKIQCHNRQFLFQKDLE 89
            K+K K + +  KE K S  + K + +K+ V ++ K  +++K  +  +  N +       E
Sbjct: 938  KQKGKDKKKKKKESKNSNMKKKEEDKKEYVNNELKKQEDNK-KETTKSENSKL----KEE 997

Query: 90   DKDNCRLTEKRYAGQSAGYSDGKFSCGSHLPEKFSVSEIALELPKRNKDEDVDSGFRNLS 149
            +KDN    EK+ +  SA  +  K     +  +K    E A +  K+++D+          
Sbjct: 998  NKDN---KEKKESEDSASKNREK---KEYEEKKSKTKEEAKKEKKKSQDKK--------- 1057

Query: 150  GEKLFACLPEKDDWMDKTMAKVTQNFNEGKTKNKNKDSREAKYGDLGIQEESKFRKRGTI 209
                     E+ D  ++   K  +   + K K K ++++E K  +    ++ + +K    
Sbjct: 1058 --------REEKDSEERKSKKEKEESRDLKAKKKEEETKEKKESENHKSKKKEDKKEHED 1117

Query: 210  QPMVKDGVDSVEKNADAKTEEKKWKGNGRDEKSKDKQKDKDKEGKGKDKERDKKKKKKEK 269
               +K   D  EK    K EE K +    D+K  +K +D++   K +DK  +KKK +  K
Sbjct: 1118 NKSMKKEEDKKEKK---KHEESKSRKKEEDKKDMEKLEDQNSNKKKEDK-NEKKKSQHVK 1177

Query: 270  IEQKFGNLKKSKERYEGDVTSPDSIITSQLLRDSHGSVASIGSLMKRKEFE 321
            + +K  + K+ KE  E   T       SQ          S     K+KE E
Sbjct: 1178 LVKKESDKKEKKENEEKSETKEIESSKSQKNEVDKKEKKSSKDQQKKKEKE 1196

HSP 5 Score: 58.2 bits (139), Expect = 1.7e-08
Identity = 68/308 (22.08%), Postives = 128/308 (41.56%), Query Frame = 1

Query: 14   KKARIEDLDLLKKDKHKKKRKKENRDHKEKKKSRSRHKRDKEKDRVKDKAKTLDESKLPD 73
            K  R  ++   K++  KKKR++  R+ K   K       + + D  K   +++   K  D
Sbjct: 862  KDDRSVEVKANKEESMKKKREEVQRNDKSSTKEVRDFANNMDIDVQKGSGESVKYKK--D 921

Query: 74   KIQCHNRQFLFQKDLEDKDNCRLTEKRYAGQSAGYSDGKFSCGSHLPEKFSVSEIALELP 133
            + +  N++       E+KD    + K+             +      E+     +  EL 
Sbjct: 922  EKKEGNKE-------ENKDTINTSSKQKGKDKKKKKKESKNSNMKKKEEDKKEYVNNELK 981

Query: 134  KR--NKDEDVDSGFRNLSGE-KLFACLPEKDDWMDKTMAKVTQNFNEGKTKNKNKDSREA 193
            K+  NK E   S    L  E K      E +D   K   K  + + E K+K K +  +E 
Sbjct: 982  KQEDNKKETTKSENSKLKEENKDNKEKKESEDSASKNREK--KEYEEKKSKTKEEAKKEK 1041

Query: 194  KYGDLGIQEESKFRKRGTIQPMVKDGVDSVEKNADAKTEEKKWKGNGRDEKSKDKQKDKD 253
            K      +EE    +R + +   ++  D   K  + +T+EKK   N + +K +DK++ +D
Sbjct: 1042 KKSQDKKREEKDSEERKSKKEK-EESRDLKAKKKEEETKEKKESENHKSKKKEDKKEHED 1101

Query: 254  KEGKGKDKERDKKKKKKEKIEQKFGNLKKSKERYEGDVTSPDSIITSQLLRDSHGSVASI 313
             +   K++++ +KKK +E   +K    KK  E+ E   ++      ++  +  H  +   
Sbjct: 1102 NKSMKKEEDKKEKKKHEESKSRKKEEDKKDMEKLEDQNSNKKKEDKNEKKKSQHVKLVKK 1157

Query: 314  GSLMKRKE 319
             S  K K+
Sbjct: 1162 ESDKKEKK 1157

HSP 6 Score: 57.0 bits (136), Expect = 3.7e-08
Identity = 71/301 (23.59%), Postives = 126/301 (41.86%), Query Frame = 1

Query: 26   KDKHKKKRKKENRDHKEKKKSRSRHKRDKEKDR-VKDKAKTLDESKLPDKIQCHNRQFLF 85
            KD    + K + ++ KE KK+++   R + K+  V+   K  ++ +  +K +  +     
Sbjct: 741  KDDKSVEAKGKKKESKENKKTKTNENRVRNKEENVQGNKKESEKVEKGEKKESKDA---- 800

Query: 86   QKDLEDKDNCRL--TEKRYAGQSAGYSDGKFSCGSHLPEKFSVSEIALELPKRNKDEDVD 145
             K +E KDN +L  TE R   +     D K        ++ S    ++E  ++N++  VD
Sbjct: 801  -KSVETKDNKKLSSTENRDEAKERSGEDNKED------KEESKDYQSVEAKEKNENGGVD 860

Query: 146  SGFRNLSGEKLFACLPEKDDWMDKTMAKVTQNFNEGKTKNKNKDSREAKYGDLGIQEESK 205
            +   N    K         D  D    +V  N  E   K + +  R  K     +++   
Sbjct: 861  TNVGNKEDSK---------DLKDDRSVEVKANKEESMKKKREEVQRNDKSSTKEVRD--- 920

Query: 206  FRKRGTIQPMVKDGVDSVEKNADAKTEEKKWKGNGR-DEKSKDKQKDKDKEGKGKDKERD 265
            F     I      G +SV+   D K E  K +     +  SK K KDK K+ K + K  +
Sbjct: 921  FANNMDIDVQKGSG-ESVKYKKDEKKEGNKEENKDTINTSSKQKGKDKKKK-KKESKNSN 980

Query: 266  KKKKKKEKIEQKFGNLKKSKERYEGDVTSPDSIITSQLL--RDSHGSVASIGSLMKRKEF 321
             KKK+++K E     LKK ++  +    S +S +  +    ++   S  S     ++KE+
Sbjct: 981  MKKKEEDKKEYVNNELKKQEDNKKETTKSENSKLKEENKDNKEKKESEDSASKNREKKEY 1016

HSP 7 Score: 56.2 bits (134), Expect = 6.3e-08
Identity = 67/297 (22.56%), Postives = 126/297 (42.42%), Query Frame = 1

Query: 13   EKKARIEDLDLLKKDKHKK-----KRKKENRDHKEKKKSRSRHKRDKEKDRVKDKAKTLD 72
            +KK   +D +  K  K K+     K KK+  + KEKK+S +   + KE  +  +  K++ 
Sbjct: 1035 DKKREEKDSEERKSKKEKEESRDLKAKKKEEETKEKKESENHKSKKKEDKKEHEDNKSMK 1094

Query: 73   ESKLPDKIQCHNRQFLFQKDLEDKDNCRLTEKRYAGQSAGYSDGKFSCGSHLPEKFSVSE 132
            + +   + + H      +K+ + KD  +L ++    +    ++ K S    L +K     
Sbjct: 1095 KEEDKKEKKKHEESKSRKKEEDKKDMEKLEDQNSNKKKEDKNEKKKSQHVKLVKK----- 1154

Query: 133  IALELPKRNKDEDVDSGFRNLSGEKLFACLPEKDDWMDKTMAKVTQNFNEGKTKNKNKDS 192
               E  K+ K E+                  EK +  +   +K  +N  + K K  +KD 
Sbjct: 1155 ---ESDKKEKKEN-----------------EEKSETKEIESSKSQKNEVDKKEKKSSKDQ 1214

Query: 193  REAKYGDLGIQEESKFRKRGTIQPMVKDGVDSVEKNADAKTEEKKWKGNGRDEKSKDKQK 252
            ++ K  ++   EE K +K        +    SVE+N   K E KK K      K KD +K
Sbjct: 1215 QKKKEKEMKESEEKKLKK----NEEDRKKQTSVEENKKQK-ETKKEK-----NKPKDDKK 1274

Query: 253  DKDKEGKGKDKERDKKKKKKEKIEQKFGNLKKSKERYEGDVTSPDSIITSQLLRDSH 305
            +  K+  GK +  + + K+ E  +QK     ++  + + D +  + ++ +    DSH
Sbjct: 1275 NTTKQSGGKKESMESESKEAEN-QQK----SQATTQADSDESKNEILMQADSQADSH 1291

HSP 8 Score: 47.4 bits (111), Expect = 2.9e-05
Identity = 56/294 (19.05%), Postives = 117/294 (39.80%), Query Frame = 1

Query: 15   KARIEDLDLLKK-DKHKKKRKKENRDH--------KEKKKSRSRHKRDKEKDRVKDKAKT 74
            K + E+    K+ + HK K+K++ ++H        +E KK + +H+  K + + +DK   
Sbjct: 1061 KKKEEETKEKKESENHKSKKKEDKKEHEDNKSMKKEEDKKEKKKHEESKSRKKEEDKK-- 1120

Query: 75   LDESKLPDKIQCHNRQFLFQKDLEDKDNCRLTEKRYAGQSAGYSDGKFSCGSHLPEKFSV 134
             D  KL D  Q  N++   + + +   + +L +K    +    ++ K         K   
Sbjct: 1121 -DMEKLED--QNSNKKKEDKNEKKKSQHVKLVKKESDKKEKKENEEKSETKEIESSKSQK 1180

Query: 135  SEIALELPKRNKDEDVDSGFRNLSGEKLFACLPEKDDWMDKTMAKVTQNFNEGKTKNKNK 194
            +E+  +  K +KD+           E+      E+D     ++ +  +     K KNK K
Sbjct: 1181 NEVDKKEKKSSKDQQKKKEKEMKESEEKKLKKNEEDRKKQTSVEENKKQKETKKEKNKPK 1240

Query: 195  DSREAKYGDLGIQEESKFRKRGTIQPMVKDGVDSVEKNADAKTEEKKWKGNGRDEKSKDK 254
            D ++      G ++ES   +    +   K    + + ++D    E   + + + +   D 
Sbjct: 1241 DDKKNTTKQSGGKKESMESESKEAENQQKSQA-TTQADSDESKNEILMQADSQADSHSDS 1300

Query: 255  QKDKDKEGK------------GKDKERDKKKKKKEKIEQKFGNLKKSKERYEGD 288
            Q D D+                ++ E D+KK+      +K    K+ K + + D
Sbjct: 1301 QADSDESKNEILMQADSQATTQRNNEEDRKKQTSVAENKKQKETKEEKNKPKDD 1348

HSP 9 Score: 42.0 bits (97), Expect = 1.2e-03
Identity = 56/253 (22.13%), Postives = 109/253 (43.08%), Query Frame = 1

Query: 41  KEKKKSRSRHKRDKEKDRVKDKAKTLDESKLPDKIQCHNRQFLFQKDLEDKDNCRLTEKR 100
           ++KK  +S      + D  K+K          ++ Q +N + +  ++LE+K++ +  +  
Sbjct: 564 EQKKNDKSVEVTTNDGDHTKEKR---------EETQGNNGESVKNENLENKEDKKELKD- 623

Query: 101 YAGQSAGYSDGKFSCGSHLPEKFSVSEIALELPKRNKDEDVDSGFRNLSGEKLFACLPEK 160
              +S G    K +  + L EK   ++   +    +K  D   G  + + EK        
Sbjct: 624 --DESVG---AKTNNETSLEEKREQTQKGHDNSINSKIVDNKGGNADSNKEKEVHVGDST 683

Query: 161 DD----WMDKTMAKVTQNFNEGKT------KNKNKDSRE-AKYGDLGIQEESKFRKRGTI 220
           +D      + T ++V    N+G +      K  NKDS E  K  +   Q +SK  K    
Sbjct: 684 NDNNMESKEDTKSEVEVKKNDGSSEKGEEGKENNKDSMEDKKLENKESQTDSKDDKS--- 743

Query: 221 QPMVKDGVDSVEKNADAKTEEKKWKGNGRDEKSKDKQKDKDKEGKGKDKERDKKKKKKEK 280
              V D  +  +       ++K  +  G+ ++SK+ +K K  E + ++KE + +  KKE 
Sbjct: 744 ---VDDKQEEAQIYGGESKDDKSVEAKGKKKESKENKKTKTNENRVRNKEENVQGNKKES 795

Query: 281 IEQKFGNLKKSKE 283
            + + G  K+SK+
Sbjct: 804 EKVEKGEKKESKD 795

HSP 10 Score: 39.7 bits (91), Expect = 6.1e-03
Identity = 55/287 (19.16%), Postives = 110/287 (38.33%), Query Frame = 1

Query: 14   KKARIEDLDLLKKDKHKKKRKK----------------ENRDHKEKKKSRSRHKRDKEKD 73
            +K + + + L+KK+  KK++K+                +N   K++KKS    ++ KEK+
Sbjct: 1137 EKKKSQHVKLVKKESDKKEKKENEEKSETKEIESSKSQKNEVDKKEKKSSKDQQKKKEKE 1196

Query: 74   RVKDKAKTLDESKLPDKIQCHNRQFLFQKDLEDKDNCRLTEKRYAGQSAGYSDGKFSCGS 133
              + + K L +++   K Q    +   QK+ + + N    +K+   + +G         S
Sbjct: 1197 MKESEEKKLKKNEEDRKKQTSVEENKKQKETKKEKNKPKDDKKNTTKQSGGKKESMESES 1256

Query: 134  HLPEKFSVSEIALELPKRNKDEDV----DSGFRNLSGEKLFACLPEKDDWMDKTMAKVTQ 193
               E    S+   +        ++    DS   + S  +  +   + +  M       TQ
Sbjct: 1257 KEAENQQKSQATTQADSDESKNEILMQADSQADSHSDSQADSDESKNEILMQADSQATTQ 1316

Query: 194  NFNEGKTKN-----KNKDSREAKYGDLGIQEESKFRKRGTIQPM--VKDGVDSVEKNADA 253
              NE   K      +NK  +E K      + + K  K+ T +     K+ ++S  K A+ 
Sbjct: 1317 RNNEEDRKKQTSVAENKKQKETKEE----KNKPKDDKKNTTKQSGGKKESMESESKEAEN 1376

Query: 254  KTEEKKWKGNGRDEKSKDKQKDKDKEGKG-KDKERDKKKKKKEKIEQ 273
            + + +       DE   +     D +     D + D  + K E + Q
Sbjct: 1377 QQKSQATTQADSDESKNEILMQADSQADSHSDSQADSDESKNEILMQ 1419

BLAST of Cucsa.256650 vs. NCBI nr
Match: gi|778677798|ref|XP_011650867.1| (PREDICTED: nipped-B-like protein B isoform X1 [Cucumis sativus])

HSP 1 Score: 899.4 bits (2323), Expect = 2.6e-258
Identity = 445/475 (93.68%), Postives = 445/475 (93.68%), Query Frame = 1

Query: 1   MSRCFPFPPPGYEKKARIEDLDLLKKDKHKKKRKKENRDHKEKKKSRSRHKRDKEKDRVK 60
           MSRCFPFPPPGYEKKARIEDLDLLKKDKHKKKRKKENRDHKEKKKSRSRHKRDKEKDRVK
Sbjct: 1   MSRCFPFPPPGYEKKARIEDLDLLKKDKHKKKRKKENRDHKEKKKSRSRHKRDKEKDRVK 60

Query: 61  DKAKTLDESKLPDKIQCHNRQFLFQKDLEDKDNCRLTEKRYAGQSAGYSDGKFSCGSHLP 120
           DKAKTLDESKLPDKIQCHNRQFLFQKDLEDKDNCRLTEKRYAGQSAGYSDGKFSCGSHLP
Sbjct: 61  DKAKTLDESKLPDKIQCHNRQFLFQKDLEDKDNCRLTEKRYAGQSAGYSDGKFSCGSHLP 120

Query: 121 EKFSVSEIALELPKRNKDEDVDSGFRNLSGEKLFACLPEKDDWMDKTMAKVTQNFNEGKT 180
           EKFSVSEIALELPKRNKDEDVDSGFRNLSGEKLFACLPEKDDWMDKTMAKVTQNFNEGKT
Sbjct: 121 EKFSVSEIALELPKRNKDEDVDSGFRNLSGEKLFACLPEKDDWMDKTMAKVTQNFNEGKT 180

Query: 181 KNKNKDSREAKYGDLGIQEESKFRKRGTIQPMVKDGVDSVEKNADAKTEEKKWKGNGRDE 240
           KNKNKDSREAKYGDLGIQEESKFRKRGTIQPMVKDGVDSVEKNADAKTEEKKWKGNGRDE
Sbjct: 181 KNKNKDSREAKYGDLGIQEESKFRKRGTIQPMVKDGVDSVEKNADAKTEEKKWKGNGRDE 240

Query: 241 KSKDKQKDKDKEGKGKDKERDKKKKKKEKIEQKFGNLKKSKERYEGDVTSPDSIITSQLL 300
           KSKDKQKDKDKEGKGKDKERDKKKKKKEKIEQKFGNLKKSKERYEGDVTSPDSIITSQLL
Sbjct: 241 KSKDKQKDKDKEGKGKDKERDKKKKKKEKIEQKFGNLKKSKERYEGDVTSPDSIITSQLL 300

Query: 301 RDSHGSVASIGSLMKRKEFENNGIFGESIGPFLLKLSRMALFYYLTDKFLLTFSTAKDHM 360
           RDSHGSVASIGSLMKRKEFENNGIF                              AKDHM
Sbjct: 301 RDSHGSVASIGSLMKRKEFENNGIF------------------------------AKDHM 360

Query: 361 SSNSTKFTSSSDLPRVNHKMLKSCQSSAPTASNWQVQSKPPHPDLECLSQVYLVPKMEEW 420
           SSNSTKFTSSSDLPRVNHKMLKSCQSSAPTASNWQVQSKPPHPDLECLSQVYLVPKMEEW
Sbjct: 361 SSNSTKFTSSSDLPRVNHKMLKSCQSSAPTASNWQVQSKPPHPDLECLSQVYLVPKMEEW 420

Query: 421 SDFCDQDWLYSSSASKLKKLNAHRYEMETTTPYVWAEAMQIDSIDICALPYVVPY 476
           SDFCDQDWLYSSSASKLKKLNAHRYEMETTTPYVWAEAMQIDSIDICALPYVVPY
Sbjct: 421 SDFCDQDWLYSSSASKLKKLNAHRYEMETTTPYVWAEAMQIDSIDICALPYVVPY 445

BLAST of Cucsa.256650 vs. NCBI nr
Match: gi|778677801|ref|XP_004134372.2| (PREDICTED: nipped-B-like protein B isoform X2 [Cucumis sativus])

HSP 1 Score: 891.0 bits (2301), Expect = 9.4e-256
Identity = 443/475 (93.26%), Postives = 443/475 (93.26%), Query Frame = 1

Query: 1   MSRCFPFPPPGYEKKARIEDLDLLKKDKHKKKRKKENRDHKEKKKSRSRHKRDKEKDRVK 60
           MSRCFPFPPPGYEKKARIEDLDLLKKDKHKKKRKKENRDHKEKKKSRSRHKRDKEKDRVK
Sbjct: 1   MSRCFPFPPPGYEKKARIEDLDLLKKDKHKKKRKKENRDHKEKKKSRSRHKRDKEKDRVK 60

Query: 61  DKAKTLDESKLPDKIQCHNRQFLFQKDLEDKDNCRLTEKRYAGQSAGYSDGKFSCGSHLP 120
           DKAKTLDESKLPDKIQCHNRQFLFQKDLEDKDNCRLTEKRYAGQSAGYSDGKFSCGSHLP
Sbjct: 61  DKAKTLDESKLPDKIQCHNRQFLFQKDLEDKDNCRLTEKRYAGQSAGYSDGKFSCGSHLP 120

Query: 121 EKFSVSEIALELPKRNKDEDVDSGFRNLSGEKLFACLPEKDDWMDKTMAKVTQNFNEGKT 180
           EKFSVSEIALELPKRNKDEDVDSGFRNLSGEKLFACLPEKDDWMDKTMAKVTQNFNEGKT
Sbjct: 121 EKFSVSEIALELPKRNKDEDVDSGFRNLSGEKLFACLPEKDDWMDKTMAKVTQNFNEGKT 180

Query: 181 KNKNKDSREAKYGDLGIQEESKFRKRGTIQPMVKDGVDSVEKNADAKTEEKKWKGNGRDE 240
           KNKNKDSREAKYGDLGIQEESKFRKRGTIQPMVKDGVDSVEKNADAKTEEKKWKGNGRDE
Sbjct: 181 KNKNKDSREAKYGDLGIQEESKFRKRGTIQPMVKDGVDSVEKNADAKTEEKKWKGNGRDE 240

Query: 241 KSKDKQKDKDKEGKGKDKERDKKKKKKEKIEQKFGNLKKSKERYEGDVTSPDSIITSQLL 300
           KSKDKQKDKDKEGKGKDKERDKKKKKKEKIEQKFGNLKKSKERYEGDVTSPDSIITSQLL
Sbjct: 241 KSKDKQKDKDKEGKGKDKERDKKKKKKEKIEQKFGNLKKSKERYEGDVTSPDSIITSQLL 300

Query: 301 RDSHGSVASIGSLMKRKEFENNGIFGESIGPFLLKLSRMALFYYLTDKFLLTFSTAKDHM 360
           RDSHGSVASIGSLMKRKEFENNGIF                              AKDHM
Sbjct: 301 RDSHGSVASIGSLMKRKEFENNGIF------------------------------AKDHM 360

Query: 361 SSNSTKFTSSSDLPRVNHKMLKSCQSSAPTASNWQVQSKPPHPDLECLSQVYLVPKMEEW 420
           SSNSTKFTSSSDLPRVNHKMLKSCQSSAPTASNW  QSKPPHPDLECLSQVYLVPKMEEW
Sbjct: 361 SSNSTKFTSSSDLPRVNHKMLKSCQSSAPTASNW--QSKPPHPDLECLSQVYLVPKMEEW 420

Query: 421 SDFCDQDWLYSSSASKLKKLNAHRYEMETTTPYVWAEAMQIDSIDICALPYVVPY 476
           SDFCDQDWLYSSSASKLKKLNAHRYEMETTTPYVWAEAMQIDSIDICALPYVVPY
Sbjct: 421 SDFCDQDWLYSSSASKLKKLNAHRYEMETTTPYVWAEAMQIDSIDICALPYVVPY 443

BLAST of Cucsa.256650 vs. NCBI nr
Match: gi|659075778|ref|XP_008438324.1| (PREDICTED: DNA ligase 1-like isoform X1 [Cucumis melo])

HSP 1 Score: 845.5 bits (2183), Expect = 4.5e-242
Identity = 421/480 (87.71%), Postives = 435/480 (90.62%), Query Frame = 1

Query: 1   MSRCFPFPPPGYEKKARIEDLDLLKKDKHKKKRKKENRDHKEKKKSRSRHKRDKEKDRVK 60
           MSRCFPFPPPGYEKKARIEDLD LKKDKHKKKRKKENRDHKEKKKSRSRHKRDKEKDRVK
Sbjct: 1   MSRCFPFPPPGYEKKARIEDLDPLKKDKHKKKRKKENRDHKEKKKSRSRHKRDKEKDRVK 60

Query: 61  DKAKTLDESKLPDKIQCHNRQFLFQKDLEDKDNCRLTEKRYAGQSAGYSDGKFSCGSHLP 120
           DKA+TLDESKLPDKIQCHNRQFLFQKDLEDKDNCRLTEK+YAGQSAGYS+GKFS GSHLP
Sbjct: 61  DKARTLDESKLPDKIQCHNRQFLFQKDLEDKDNCRLTEKKYAGQSAGYSEGKFSYGSHLP 120

Query: 121 EKFSVSEIALELPKRNKDEDVDSGFRNLSGEKLFACLPEKDDWMDKTMAKVTQNFNEGKT 180
           EKFSVSEIALELPKRNKDEDVDSGFRNL GEKL  CLPE++DWMDKTMAKVTQNF EGKT
Sbjct: 121 EKFSVSEIALELPKRNKDEDVDSGFRNLLGEKLSTCLPERNDWMDKTMAKVTQNFIEGKT 180

Query: 181 KNKNKDSREAKYGDLGIQEESKFRKRGTIQPMVKDGVDSVEKNADAKTEEKKWKGNGRDE 240
           KNKNKDSREAKYGDLGI+EESKF KRGTIQP VKDGVDSVEKNADA+TEEKKWKG+G+DE
Sbjct: 181 KNKNKDSREAKYGDLGIREESKFSKRGTIQPTVKDGVDSVEKNADARTEEKKWKGDGKDE 240

Query: 241 KSKDKQKDKD-----KEGKGKDKERDKKKKKKEKIEQKFGNLKKSKERYEGDVTSPDSII 300
           KSK+K+KDKD     KEGKGKDKERDKKKKKKEKIEQKFGNL+KSKERYEGDVTSPDSII
Sbjct: 241 KSKEKRKDKDKEGKGKEGKGKDKERDKKKKKKEKIEQKFGNLEKSKERYEGDVTSPDSII 300

Query: 301 TSQLLRDSHGSVASIGSLMKRKEFENNGIFGESIGPFLLKLSRMALFYYLTDKFLLTFST 360
           TSQLLRDSHGSVAS+GSLMKRKEFE NGIF                              
Sbjct: 301 TSQLLRDSHGSVASMGSLMKRKEFEINGIF------------------------------ 360

Query: 361 AKDHMSSNSTKFTSSSDLPRVNHKMLKSCQSSAPTASNWQVQSKPPHPDLECLSQVYLVP 420
           AKDHMSSNSTKFTSSSDLPRV HKMLKSCQSSAPTASNWQVQSKPPHPDLECLSQVYLVP
Sbjct: 361 AKDHMSSNSTKFTSSSDLPRVKHKMLKSCQSSAPTASNWQVQSKPPHPDLECLSQVYLVP 420

Query: 421 KMEEWSDFCDQDWLYSSSASKLKKLNAHRYEMETTTPYVWAEAMQIDSIDICALPYVVPY 476
           KMEEWSDFCDQDWLYSSSASKLKKLNAHR+EMETTTPYVWAEAMQIDSIDICALPYVVPY
Sbjct: 421 KMEEWSDFCDQDWLYSSSASKLKKLNAHRFEMETTTPYVWAEAMQIDSIDICALPYVVPY 450

BLAST of Cucsa.256650 vs. NCBI nr
Match: gi|659075780|ref|XP_008438325.1| (PREDICTED: DNA ligase 1-like isoform X2 [Cucumis melo])

HSP 1 Score: 837.0 bits (2161), Expect = 1.6e-239
Identity = 419/480 (87.29%), Postives = 433/480 (90.21%), Query Frame = 1

Query: 1   MSRCFPFPPPGYEKKARIEDLDLLKKDKHKKKRKKENRDHKEKKKSRSRHKRDKEKDRVK 60
           MSRCFPFPPPGYEKKARIEDLD LKKDKHKKKRKKENRDHKEKKKSRSRHKRDKEKDRVK
Sbjct: 1   MSRCFPFPPPGYEKKARIEDLDPLKKDKHKKKRKKENRDHKEKKKSRSRHKRDKEKDRVK 60

Query: 61  DKAKTLDESKLPDKIQCHNRQFLFQKDLEDKDNCRLTEKRYAGQSAGYSDGKFSCGSHLP 120
           DKA+TLDESKLPDKIQCHNRQFLFQKDLEDKDNCRLTEK+YAGQSAGYS+GKFS GSHLP
Sbjct: 61  DKARTLDESKLPDKIQCHNRQFLFQKDLEDKDNCRLTEKKYAGQSAGYSEGKFSYGSHLP 120

Query: 121 EKFSVSEIALELPKRNKDEDVDSGFRNLSGEKLFACLPEKDDWMDKTMAKVTQNFNEGKT 180
           EKFSVSEIALELPKRNKDEDVDSGFRNL GEKL  CLPE++DWMDKTMAKVTQNF EGKT
Sbjct: 121 EKFSVSEIALELPKRNKDEDVDSGFRNLLGEKLSTCLPERNDWMDKTMAKVTQNFIEGKT 180

Query: 181 KNKNKDSREAKYGDLGIQEESKFRKRGTIQPMVKDGVDSVEKNADAKTEEKKWKGNGRDE 240
           KNKNKDSREAKYGDLGI+EESKF KRGTIQP VKDGVDSVEKNADA+TEEKKWKG+G+DE
Sbjct: 181 KNKNKDSREAKYGDLGIREESKFSKRGTIQPTVKDGVDSVEKNADARTEEKKWKGDGKDE 240

Query: 241 KSKDKQKDKD-----KEGKGKDKERDKKKKKKEKIEQKFGNLKKSKERYEGDVTSPDSII 300
           KSK+K+KDKD     KEGKGKDKERDKKKKKKEKIEQKFGNL+KSKERYEGDVTSPDSII
Sbjct: 241 KSKEKRKDKDKEGKGKEGKGKDKERDKKKKKKEKIEQKFGNLEKSKERYEGDVTSPDSII 300

Query: 301 TSQLLRDSHGSVASIGSLMKRKEFENNGIFGESIGPFLLKLSRMALFYYLTDKFLLTFST 360
           TSQLLRDSHGSVAS+GSLMKRKEFE NGIF                              
Sbjct: 301 TSQLLRDSHGSVASMGSLMKRKEFEINGIF------------------------------ 360

Query: 361 AKDHMSSNSTKFTSSSDLPRVNHKMLKSCQSSAPTASNWQVQSKPPHPDLECLSQVYLVP 420
           AKDHMSSNSTKFTSSSDLPRV HKMLKSCQSSAPTASNW  QSKPPHPDLECLSQVYLVP
Sbjct: 361 AKDHMSSNSTKFTSSSDLPRVKHKMLKSCQSSAPTASNW--QSKPPHPDLECLSQVYLVP 420

Query: 421 KMEEWSDFCDQDWLYSSSASKLKKLNAHRYEMETTTPYVWAEAMQIDSIDICALPYVVPY 476
           KMEEWSDFCDQDWLYSSSASKLKKLNAHR+EMETTTPYVWAEAMQIDSIDICALPYVVPY
Sbjct: 421 KMEEWSDFCDQDWLYSSSASKLKKLNAHRFEMETTTPYVWAEAMQIDSIDICALPYVVPY 448

BLAST of Cucsa.256650 vs. NCBI nr
Match: gi|731390351|ref|XP_010650330.1| (PREDICTED: pre-mRNA-splicing factor CWC22 homolog [Vitis vinifera])

HSP 1 Score: 298.9 bits (764), Expect = 1.6e-77
Identity = 211/529 (39.89%), Postives = 284/529 (53.69%), Query Frame = 1

Query: 1   MSRCFPFPPPGYEKKARIEDLDLLKKDKHK-KKRKKENRD------------------HK 60
           MSRCFPFPPPGYEKKAR +D DLLKK+KH+ KK KKE R+                  HK
Sbjct: 1   MSRCFPFPPPGYEKKARTDDADLLKKEKHREKKHKKEKREKEKKEGKEKREKDRGDGRHK 60

Query: 61  EKKKSRSRH---KRDKEKDRVKDKAK--TLDESKLPDKIQCHNRQFLFQKDLEDKD-NCR 120
           EKK  + +H   K+DKEKDR K+K K  T DE +L  + +  N + L QK++ D+D N  
Sbjct: 61  EKKDRKDKHRDRKKDKEKDREKEKDKNITSDEKRLASEFEGFNAEKLSQKEVRDRDKNSV 120

Query: 121 LTEKRYAGQSAGYSDGKFSCGSHLPEKFSVSEIALELPKRNKDEDVDSGFRNLSGEKLFA 180
             EKR+AGQ  GY++ K +  SHL E    S+   EL +R + ED  +G  N   EK   
Sbjct: 121 SNEKRFAGQLVGYNEEKLTQSSHLGEGNKDSKFLQELGRRIRAEDGGNG--NQLVEKFTG 180

Query: 181 CLPEKDDWMDKTMAKVTQNFNEGKTKNKNKDSREAKYGDLGIQEESKFRKRGTIQPMVKD 240
               KD+ M + +AK      EGK KNK+K   + K    G+++ES+       Q +   
Sbjct: 181 TDRRKDEAMARLVAKDVGIRTEGKEKNKDKRGEDRKIDGQGVRDESRISGNSVAQSLTGT 240

Query: 241 GV-------DSVEKNADAKTEEK-KWKGNGRDEKSKDKQKDKDKEGK--GKDKERDKKKK 300
           G+         +EK  + + E K K K    D+K  DK+KD+D+E K  GK+K+RDK+K+
Sbjct: 241 GLHRLEGTPKPLEKKIERRMEAKEKTKDKEGDDKRGDKRKDRDREKKSQGKEKDRDKEKR 300

Query: 301 K----KEKIEQKFGNLKKSKERYEGDVTSPDSIITSQLLRDSHGSVASIGSLMKRKEFEN 360
           K    KEKIE K   L KSKE  + DVT   +I TS L +DS+ S A+ G+  KRK+ + 
Sbjct: 301 KEEKEKEKIEHKNKELDKSKESNKDDVTVTHNIKTSHLHKDSNKSAATEGNHRKRKDSDT 360

Query: 361 NG-IFGESIGPFLLKLSRMALFYYLTD--------KFLLTFSTAKDHMSSNSTKFTSSSD 420
           NG +    + P   KL R      + +          +L  S  +   ++N         
Sbjct: 361 NGFLHVNEVKP--NKLPRPTSHPLIENGRKLEPCQTSILITSDRQGGTANNLKVENKEHK 420

Query: 421 LPRVNHKMLKSCQSSAPTASNWQ------VQSKPPHPDLECLSQVYLVPKMEEWSDFCDQ 476
           L  +      S  S+ P +   Q         KPPHPD + LSQV LVPKMEEWSDF DQ
Sbjct: 421 LNGIIGAQPFSVASTKPLSVASQADQIADASKKPPHPDSKYLSQVLLVPKMEEWSDFDDQ 480