Cucsa.252590 (gene) Cucumber (Gy14) v1

Overview
NameCucsa.252590
Typegene
OrganismCucumis sativus (Cucumber (Gy14) v1)
DescriptionUbiquitin-conjugating enzyme, E2
Locationscaffold02229 : 1491966 .. 1494455 (-)
Genome Viewer
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: five_prime_UTRCDSthree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
TTGATTATCGATTCAAATCCTCTCGTTTGGTTCCGGAGATTGCTTCTTTTCCGATCAAGTAATTTTGTAGTCTCGTGATCGGTGGTGGTTGCAGTCGGATCGGATCTGCGATTGAATTGAGAAGCTATGGCGGAGGATAAGTACAATTTGAAGAACCCTGCGGTGAAGAGGATCCTTCAGGAGGTCAAGGAGATGCAGGCTAATCCTTCTGATGATTTGATGAGTCTTCCTCTTGAGGTTTGTTGCTTATGATCTTATCTGAATTTCCATTCCTGTTTTAAAATTTAACCTAATGGGTGTTGTTCTTGTTTGGTCTGTTTTACATTTCAATGGTTTCCGTTTCTCTGTTGAATGGATTGTTTCATTGTGATTGTCCCCGATTGTTGAAATGGCACGATGTATGTGGAAGTGTGGACGGAAATCAATTATCAATTTGGTGTTCTTTCATTTTTCTTACCTACTCTACTGGGGTCTGCGACTAGTCGCATATGAATGATCCATTAGGGCTCAAAAAGGAAGAAAGTGAAAACATTAACCAGTTTTTTTGGGTTAAATTTTTATTATACTATTCATTCTAGTTAACGATCTTCGAAAATTATCGACGGCTGAAAGTTTGGAGCAGGCCTTTGTTTTCAATAAACCCTTGTTCTCTAGCTAGGGTAATAATCATTTTGTTTAAATTGTTTGCTAAATCTACGAACACATGCGCATTCGGTCTTTTTGCATAAATCTCCATTTATCTTATACATGTGTGTCGACTTCATTGCATTTCGGATAAAGAAAAGCAAAAGAGAGGAAATATCGGCTATGGGAACTAGGAAGGTTGCTTGATTGATACACCAATCTTATAATTATATGCATAATTTTTGTATTTCAATTGTTATAGAATTTAGGGGTTTTTCCAAACCTCCCTGTTGAATTTGAGGCTAAATGTTGGTTTTTCAGGAGAATATATTTGAATGGCAATTTGCCATCCGCGGACCTGGTGATAGTGAGTTTGAGGGTGGGATATATCATGGACGTATCCAATTGCCAGCAGAATATCCATTCAAGCCACCTTCATTCATGCTATTGACCGTAAGATAGTGATCTCTCGTCATGCTTGTTTCTCTTTCTGTTGCTTCTCGGCTTATATAGAGCATGTTTGGAATCACTTTTCATATCTAATACACTTTTAAACACAATTTTTTGTAAGATCAAAATTCAGTTTTAAAGATTAGAAGTGATCTCTCAGATTGAATAGAAGACTAGGATTGTTCTGGCAATTTCATAATCACTCTCAAACAAGCCTCCTATAGTTCTTCTGTTGCGTATTTTGACAAGTGATGTTATACTTAATGGAAGAGCTTATCAATGTCAATTCCTTTCTTTCAGCCGAACGGTCGATTTGAAACCCAAACGAAGATTTGTTTGAGCATATCAAATCACCATCCAGAGCATTGGCAACCATCATGGAGTGGTGCGTTCTTTTTATTATTTATTAAACTTGTCTTGTCATCTTATGTTATGCATTGTCTGGATTTTGCATTTACACTTAGTTGCTTCAAGTTCCACATGCTCTGATCTCCGTTCCTTTTTTCCTGGCTTCCCCATTTCTGTTGATTACTGTTTGTAGTGCGCACTGCGTTGGTGGCATTAATTGCTTTCATGCCTACAAACCCAAATGGAGCTCTTGGTTCCCTGGACTACAAGAAGGAAGAAAGGCGAGTACTGGCCATCAAATCTCGAGAAGCACCCCCGAAATTTGGTACTCCTGAACGTCAAAAACTAATTGACGAGGTACTCCCAAGTTCTGTTGAAATTTTATAAGCTATCAAGTAGCAATAATGCATTGAATCTCCAACATGTGATATGTCATTGTTAACTAATCAGCTTCCTTTACATCTCAGATTCATGAATACATGCTGAGTAAGGCGCCGCCTGTCCCTCAATCGGGTTCTACAGATGTCTCTGGCGAGAAACCCACCAACGAGGAGGCTGATGTCCAGGTAACTTCTGAAACTGCTGAACTTGCAGCTGCTGTGGAAGAGCTGCAGAATTCACCTACAATCAACAGGATCGGTGAGGAAGCAGTACCAGAGGTCCAGCCAGAAGTGGCAAGAGTGGCGCCATCTAGTCGCCCCACCACTCAGACTGAGCAGGGGAGGCCTGAGACAAGAGTTCATAAACCAGCCGATGACAGGTTATTCACATGGGCTGCAATCGGGCTCACTCTTGCAATTGTTGTTCTTTTGCTGAAGAAGTTCATGAAAGCAAGCGCTCATGGTTCTGTGTTCATGGATGAATCTTAGAAGATTGGAGATGATATTTGTTGGTTGATAGCTTTGCTATGAGACACAAGGGTCTTATGTGTATTGATAGTTTTAGCGTAAATTCTAATGTTTCTTATCATTCTGATTCTACAAGGAAAAAAGCAATCTTATGTTTTTATGGTGTTGCCTTCTCCATTATTTGTAGTTAAATAATAACATATGCAAAATTGTTTGGCTT

mRNA sequence

TTGATTATCGATTCAAATCCTCTCGTTTGGTTCCGGAGATTGCTTCTTTTCCGATCAAGTAATTTTGTAGTCTCGTGATCGGTGGTGGTTGCAGTCGGATCGGATCTGCGATTGAATTGAGAAGCTATGGCGGAGGATAAGTACAATTTGAAGAACCCTGCGGTGAAGAGGATCCTTCAGGAGGTCAAGGAGATGCAGGCTAATCCTTCTGATGATTTGATGAGTCTTCCTCTTGAGGAGAATATATTTGAATGGCAATTTGCCATCCGCGGACCTGGTGATAGTGAGTTTGAGGGTGGGATATATCATGGACGTATCCAATTGCCAGCAGAATATCCATTCAAGCCACCTTCATTCATGCTATTGACCCCGAACGGTCGATTTGAAACCCAAACGAAGATTTGTTTGAGCATATCAAATCACCATCCAGAGCATTGGCAACCATCATGGAGTGTGCGCACTGCGTTGGTGGCATTAATTGCTTTCATGCCTACAAACCCAAATGGAGCTCTTGGTTCCCTGGACTACAAGAAGGAAGAAAGGCGAGTACTGGCCATCAAATCTCGAGAAGCACCCCCGAAATTTGGTACTCCTGAACGTCAAAAACTAATTGACGAGATTCATGAATACATGCTGAGTAAGGCGCCGCCTGTCCCTCAATCGGGTTCTACAGATGTCTCTGGCGAGAAACCCACCAACGAGGAGGCTGATGTCCAGGTAACTTCTGAAACTGCTGAACTTGCAGCTGCTGTGGAAGAGCTGCAGAATTCACCTACAATCAACAGGATCGGTGAGGAAGCAGTACCAGAGGTCCAGCCAGAAGTGGCAAGAGTGGCGCCATCTAGTCGCCCCACCACTCAGACTGAGCAGGGGAGGCCTGAGACAAGAGTTCATAAACCAGCCGATGACAGGTTATTCACATGGGCTGCAATCGGGCTCACTCTTGCAATTGTTGTTCTTTTGCTGAAGAAGTTCATGAAAGCAAGCGCTCATGGTTCTGTGTTCATGGATGAATCTTAGAAGATTGGAGATGATATTTGTTGGTTGATAGCTTTGCTATGAGACACAAGGGTCTTATGTGTATTGATAGTTTTAGCGTAAATTCTAATGTTTCTTATCATTCTGATTCTACAAGGAAAAAAGCAATCTTATGTTTTTATGGTGTTGCCTTCTCCATTATTTGTAGTTAAATAATAACATATGCAAAATTGTTTGGCTT

Coding sequence (CDS)

ATGGCGGAGGATAAGTACAATTTGAAGAACCCTGCGGTGAAGAGGATCCTTCAGGAGGTCAAGGAGATGCAGGCTAATCCTTCTGATGATTTGATGAGTCTTCCTCTTGAGGAGAATATATTTGAATGGCAATTTGCCATCCGCGGACCTGGTGATAGTGAGTTTGAGGGTGGGATATATCATGGACGTATCCAATTGCCAGCAGAATATCCATTCAAGCCACCTTCATTCATGCTATTGACCCCGAACGGTCGATTTGAAACCCAAACGAAGATTTGTTTGAGCATATCAAATCACCATCCAGAGCATTGGCAACCATCATGGAGTGTGCGCACTGCGTTGGTGGCATTAATTGCTTTCATGCCTACAAACCCAAATGGAGCTCTTGGTTCCCTGGACTACAAGAAGGAAGAAAGGCGAGTACTGGCCATCAAATCTCGAGAAGCACCCCCGAAATTTGGTACTCCTGAACGTCAAAAACTAATTGACGAGATTCATGAATACATGCTGAGTAAGGCGCCGCCTGTCCCTCAATCGGGTTCTACAGATGTCTCTGGCGAGAAACCCACCAACGAGGAGGCTGATGTCCAGGTAACTTCTGAAACTGCTGAACTTGCAGCTGCTGTGGAAGAGCTGCAGAATTCACCTACAATCAACAGGATCGGTGAGGAAGCAGTACCAGAGGTCCAGCCAGAAGTGGCAAGAGTGGCGCCATCTAGTCGCCCCACCACTCAGACTGAGCAGGGGAGGCCTGAGACAAGAGTTCATAAACCAGCCGATGACAGGTTATTCACATGGGCTGCAATCGGGCTCACTCTTGCAATTGTTGTTCTTTTGCTGAAGAAGTTCATGAAAGCAAGCGCTCATGGTTCTGTGTTCATGGATGAATCTTAG

Protein sequence

MAEDKYNLKNPAVKRILQEVKEMQANPSDDLMSLPLEENIFEWQFAIRGPGDSEFEGGIYHGRIQLPAEYPFKPPSFMLLTPNGRFETQTKICLSISNHHPEHWQPSWSVRTALVALIAFMPTNPNGALGSLDYKKEERRVLAIKSREAPPKFGTPERQKLIDEIHEYMLSKAPPVPQSGSTDVSGEKPTNEEADVQVTSETAELAAAVEELQNSPTINRIGEEAVPEVQPEVARVAPSSRPTTQTEQGRPETRVHKPADDRLFTWAAIGLTLAIVVLLLKKFMKASAHGSVFMDES*
Homology
[Close]
BLAST of Cucsa.252590 vs. Swiss-Prot
Match: UBC32_ARATH (Ubiquitin-conjugating enzyme E2 32 OS=Arabidopsis thaliana GN=UBC32 PE=2 SV=1)

HSP 1 Score: 393.7 bits (1010), Expect = 1.8e-108
Identity = 201/309 (65.05%), Postives = 234/309 (75.73%), Query Frame = 1 

Query: 1   MAEDKYNLKNPAVKRILQEVKEMQANPSDDLMSLPLEENIFEWQFAIRGPGDSEFEGGIY 60
           MA+++YN KNPAVKRILQEVKEMQANPSDD MSLPLEENIFEWQFAIRGPGD+EFEGGIY
Sbjct: 1   MADERYNRKNPAVKRILQEVKEMQANPSDDFMSLPLEENIFEWQFAIRGPGDTEFEGGIY 60

Query: 61  HGRIQLPAEYPFKPPSFMLLTPNGRFETQTKICLSISNHHPEHWQPSWSVRTALVALIAF 120
           HGRIQLPA+YPFKPPSFMLLTPNGRFET TKICLSISN+HPEHWQPSWSVRTALVALIAF
Sbjct: 61  HGRIQLPADYPFKPPSFMLLTPNGRFETNTKICLSISNYHPEHWQPSWSVRTALVALIAF 120

Query: 121 MPTNPNGALGSLDYKKEERRVLAIKSREAPPKFGTPERQKLIDEIHEYMLSKAPPVPQSG 180
           MPT+PNGALGS+DY K+ERR LAIKSRE PPK+G+PERQK+IDEIH+Y+LSKA  VP+  
Sbjct: 121 MPTSPNGALGSVDYPKDERRTLAIKSRETPPKYGSPERQKIIDEIHQYILSKATVVPKPL 180

Query: 181 STDVSGEKPTNEEADVQVTSETAELAAAVEELQNSPTINRIGEEAVPEVQPEV---ARVA 240
             + S       EA  QV  E  E    VEE   + T   + ++ + E    V   A V 
Sbjct: 181 PLECSQAPSIVSEAHSQV--EPQEAITVVEERSIATTDTIVDDQIIEETAEAVNTAASVV 240

Query: 241 PSSRP----------TTQTEQGRPETRVHKPADDRLFTWAAIGLTLAIVVLLLKKFMKAS 297
           P++ P          +   EQ        KP DDRLFTWAA+GLT+AI+VLLLKKF+K++
Sbjct: 241 PAAAPLPAVEVVVKASVSGEQRMARRAAQKPVDDRLFTWAAVGLTIAIMVLLLKKFIKSN 300

[Close]
BLAST of Cucsa.252590 vs. Swiss-Prot
Match: UB2J1_MOUSE (Ubiquitin-conjugating enzyme E2 J1 OS=Mus musculus GN=Ube2j1 PE=1 SV=2)

HSP 1 Score: 207.6 bits (527), Expect = 1.9e-52
Identity = 131/317 (41.32%), Postives = 176/317 (55.52%), Query Frame = 1 

Query: 3   EDKYNLKNPAVKRILQEVKEMQANPSDDLMSLPLEENIFEWQFAIRGPGDSEFEGGIYHG 62
           E +YNLK+PAVKR+++E  E++ +P+D   + PLE+N+FEW F +RGP DS+F+GG+YHG
Sbjct: 2   ETRYNLKSPAVKRLMKEAAELK-DPTDHYHAQPLEDNLFEWHFTVRGPPDSDFDGGVYHG 61

Query: 63  RIQLPAEYPFKPPSFMLLTPNGRFETQTKICLSISNHHPEHWQPSWSVRTALVALIAFMP 122
           RI LP EYP KPPS +LLT NGRFE   KICLSIS HHPE WQPSWS+RTAL+A+I FMP
Sbjct: 62  RIVLPPEYPMKPPSIILLTANGRFEVGKKICLSISGHHPETWQPSWSIRTALLAIIGFMP 121

Query: 123 TNPNGALGSLDYKKEERRVLAIKSRE--------------APPKFGTPERQ--KLIDEIH 182
           T   GA+GSLDY  EERR LA KS++               P K G+   Q  +   E+ 
Sbjct: 122 TKGEGAIGSLDYTPEERRALAKKSQDFCCEGCGSAMKDVLLPLKSGSGSSQADQEAKELA 181

Query: 183 EYMLSKAPPVPQSGST----DVSGEKPTNEEADVQVTSETAELAAAVEELQNSPTINRIG 242
             +  KA  V  SG T    D++     N+  D   T+     A+     QN P+   + 
Sbjct: 182 RQISFKA-EVNSSGKTIAESDLNQCFSLNDSQDDLPTTFQGATASTSYGAQN-PSGAPLP 241

Query: 243 EEAVPEVQPEVARVAPSSRPTTQTEQGRPET----------RVHKPADDRLFTWAAIGLT 290
           +   P   P+   ++P  R   Q  Q RP T          R H   +        I LT
Sbjct: 242 QPTQP--APKNTSMSPRQRRAQQQSQRRPSTSPDVLQGQPPRAHH-TEHGGSAMLIIILT 301

[Close]
BLAST of Cucsa.252590 vs. Swiss-Prot
Match: UB2J1_HUMAN (Ubiquitin-conjugating enzyme E2 J1 OS=Homo sapiens GN=UBE2J1 PE=1 SV=2)

HSP 1 Score: 206.5 bits (524), Expect = 4.2e-52
Identity = 94/146 (64.38%), Postives = 117/146 (80.14%), Query Frame = 1 

Query: 3   EDKYNLKNPAVKRILQEVKEMQANPSDDLMSLPLEENIFEWQFAIRGPGDSEFEGGIYHG 62
           E +YNLK+PAVKR+++E  E++ +P+D   + PLE+N+FEW F +RGP DS+F+GG+YHG
Sbjct: 2   ETRYNLKSPAVKRLMKEAAELK-DPTDHYHAQPLEDNLFEWHFTVRGPPDSDFDGGVYHG 61

Query: 63  RIQLPAEYPFKPPSFMLLTPNGRFETQTKICLSISNHHPEHWQPSWSVRTALVALIAFMP 122
           RI LP EYP KPPS +LLT NGRFE   KICLSIS HHPE WQPSWS+RTAL+A+I FMP
Sbjct: 62  RIVLPPEYPMKPPSIILLTANGRFEVGKKICLSISGHHPETWQPSWSIRTALLAIIGFMP 121

Query: 123 TNPNGALGSLDYKKEERRVLAIKSRE 149
           T   GA+GSLDY  EERR LA KS++
Sbjct: 122 TKGEGAIGSLDYTPEERRALAKKSQD 146

[Close]
BLAST of Cucsa.252590 vs. Swiss-Prot
Match: UBC6_CANGA (Ubiquitin-conjugating enzyme E2 6 OS=Candida glabrata (strain ATCC 2001 / CBS 138 / JCM 3761 / NBRC 0622 / NRRL Y-65) GN=UBC6 PE=3 SV=1)

HSP 1 Score: 140.2 bits (352), Expect = 3.7e-32
Identity = 61/135 (45.19%), Postives = 92/135 (68.15%), Query Frame = 1 

Query: 12  AVKRILQEVKEMQANPSDDLMSLPLEENIFEWQFAIRGPGDSEFEGGIYHGRIQLPAEYP 71
           A KR+ +E K M  NP   +++ P EENI EW + I GP D+ ++GG YHG +  P++YP
Sbjct: 6   AQKRLTKEYKMMVENPPPFIIARPNEENILEWHYVISGPPDTPYDGGQYHGTLTFPSDYP 65

Query: 72  FKPPSFMLLTPNGRFETQTKICLSISNHHPEHWQPSWSVRTALVALIAFMPTNPNGALGS 131
           +KPP+  ++TPNGRF+  T++CLS+S++HP+ W P WSV T L  L++FM T      GS
Sbjct: 66  YKPPAIRMITPNGRFKENTRLCLSMSDYHPDTWNPGWSVATILNGLLSFM-TGDESTTGS 125

Query: 132 LDYKKEERRVLAIKS 147
           +   ++E+++LA KS
Sbjct: 126 ITTTQQEKKILAKKS 139

[Close]
BLAST of Cucsa.252590 vs. Swiss-Prot
Match: UBC6_KLULA (Ubiquitin-conjugating enzyme E2 6 OS=Kluyveromyces lactis (strain ATCC 8585 / CBS 2359 / DSM 70799 / NBRC 1267 / NRRL Y-1140 / WM37) GN=UBC6 PE=3 SV=1)

HSP 1 Score: 134.4 bits (337), Expect = 2.0e-30
Identity = 71/212 (33.49%), Postives = 115/212 (54.25%), Query Frame = 1 

Query: 12  AVKRILQEVKEMQANPSDDLMSLPLEENIFEWQFAIRGPGDSEFEGGIYHGRIQLPAEYP 71
           A KR+ +E K +  NP   +++ P E+NI EW + I GP  + +E G YHG +  P++YP
Sbjct: 6   ANKRLTKEYKNIVNNPPPFIIAAPHEDNILEWHYVITGPPSTPYENGQYHGTLTFPSDYP 65

Query: 72  FKPPSFMLLTPNGRFETQTKICLSISNHHPEHWQPSWSVRTALVALIAFMPTNPNGALGS 131
           F PP+  ++TPNGRF+  T++CLS+S++HPE W P+WSV T L  L++FM T      GS
Sbjct: 66  FNPPAIRMITPNGRFKENTRLCLSMSDYHPEAWNPAWSVVTILNGLLSFM-TGDEQTTGS 125

Query: 132 LDYKKEERRVLAIKSREAPPKFGTPERQKLIDEIHEYMLSKAPPVPQSGSTDVSGEKPTN 191
           +    +++R LA KS+     F T    K  +   +   S    + +  + +   +    
Sbjct: 126 VSTSDKDKRTLAKKSKH----FNTYSNFKFKNMFPDLRESNIKDIEREAALEAESKGAQQ 185

Query: 192 EEADVQVTSETAELAAAVEELQNSPTINRIGE 224
           EE   Q  +   E A +++++ +     RI E
Sbjct: 186 EENKAQKLA--TEKATSLDDISDPEDRVRIQE 210

[Close]
BLAST of Cucsa.252590 vs. TrEMBL
Match: A0A0A0L954_CUCSA (Uncharacterized protein OS=Cucumis sativus GN=Csa_3G166270 PE=4 SV=1)

HSP 1 Score: 593.6 bits (1529), Expect = 1.4e-166
Identity = 295/297 (99.33%), Postives = 295/297 (99.33%), Query Frame = 1 

Query: 1   MAEDKYNLKNPAVKRILQEVKEMQANPSDDLMSLPLEENIFEWQFAIRGPGDSEFEGGIY 60
           MAEDKYNLKNPAVKRILQEVKEMQANPSDD MSLPLEENIFEWQFAIRGPGDSEFEGGIY
Sbjct: 1   MAEDKYNLKNPAVKRILQEVKEMQANPSDDFMSLPLEENIFEWQFAIRGPGDSEFEGGIY 60

Query: 61  HGRIQLPAEYPFKPPSFMLLTPNGRFETQTKICLSISNHHPEHWQPSWSVRTALVALIAF 120
           HGRIQLPAEYPFKPPSFMLLTPNGRFETQTKICLSISNHHPEHWQPSWSVRTALVALIAF
Sbjct: 61  HGRIQLPAEYPFKPPSFMLLTPNGRFETQTKICLSISNHHPEHWQPSWSVRTALVALIAF 120

Query: 121 MPTNPNGALGSLDYKKEERRVLAIKSREAPPKFGTPERQKLIDEIHEYMLSKAPPVPQSG 180
           MPTNPNGALGSLDYKKEERRVLAIKSREAPPKFGTPERQKLIDEIHEYMLSKAPPVPQSG
Sbjct: 121 MPTNPNGALGSLDYKKEERRVLAIKSREAPPKFGTPERQKLIDEIHEYMLSKAPPVPQSG 180

Query: 181 STDVSGEKPTNEEADVQVTSETAELAAAVEELQNSPTINRIGEEAVPEVQPEVARVAPSS 240
           STDVSGEKPTNEEADVQVTSETAELAAAVEELQNSPTINRI EEAVPEVQPEVARVAPSS
Sbjct: 181 STDVSGEKPTNEEADVQVTSETAELAAAVEELQNSPTINRIVEEAVPEVQPEVARVAPSS 240

Query: 241 RPTTQTEQGRPETRVHKPADDRLFTWAAIGLTLAIVVLLLKKFMKASAHGSVFMDES 298
           RPTTQTEQGRPETRVHKPADDRLFTWAAIGLTLAIVVLLLKKFMKASAHGSVFMDES
Sbjct: 241 RPTTQTEQGRPETRVHKPADDRLFTWAAIGLTLAIVVLLLKKFMKASAHGSVFMDES 297

[Close]
BLAST of Cucsa.252590 vs. TrEMBL
Match: V4RY15_9ROSI (Uncharacterized protein OS=Citrus clementina GN=CICLE_v10005518mg PE=4 SV=1)

HSP 1 Score: 449.1 bits (1154), Expect = 4.1e-123
Identity = 233/306 (76.14%), Postives = 255/306 (83.33%), Query Frame = 1 

Query: 1   MAEDKYNLKNPAVKRILQEVKEMQANPSDDLMSLPLEENIFEWQFAIRGPGDSEFEGGIY 60
           MAED+YNLKNPAVKRILQEVKEMQ+NPSDD MSLPLEENIFEWQFAIRGPGD+EFEGGIY
Sbjct: 1   MAEDRYNLKNPAVKRILQEVKEMQSNPSDDFMSLPLEENIFEWQFAIRGPGDTEFEGGIY 60

Query: 61  HGRIQLPAEYPFKPPSFMLLTPNGRFETQTKICLSISNHHPEHWQPSWSVRTALVALIAF 120
           HGRIQLPAEYPFKPPSFMLLTPNGRFETQTKICLSISNHHPEHWQPSWSVRTALVALIAF
Sbjct: 61  HGRIQLPAEYPFKPPSFMLLTPNGRFETQTKICLSISNHHPEHWQPSWSVRTALVALIAF 120

Query: 121 MPTNPNGALGSLDYKKEERRVLAIKSREAPPKFGTPERQKLIDEIHEYMLSKAPPVPQSG 180
           MPTNPNGALGSLDYKKEERR LAIKSREA PKFGTPERQKLIDEIHEYMLSKAPPVPQ  
Sbjct: 121 MPTNPNGALGSLDYKKEERRALAIKSREAAPKFGTPERQKLIDEIHEYMLSKAPPVPQLS 180

Query: 181 STDVSGEKPTNEEADVQVTSETAELAAAVEELQNSPTINRIGEEAVPEV------QPEVA 240
           + +   E+P N E + Q +S  A +A A E L  +P  +RI EE   ++       P VA
Sbjct: 181 TCE---EQPGNREGEAQASSIDAMVAGAGEGLP-APVGDRIIEEVQEDLPASMNPNPVVA 240

Query: 241 ---RVAPSSRPTTQTEQGRPETRVHKPADDRLFTWAAIGLTLAIVVLLLKKFMKASAHGS 298
              R  P++    Q  Q +PETRV KPADDRLFTWAA+GLT+AI+VLLLKKFMK++ HG+
Sbjct: 241 GTSREVPANASGVQL-QPKPETRVQKPADDRLFTWAAVGLTIAILVLLLKKFMKSNGHGA 300

[Close]
BLAST of Cucsa.252590 vs. TrEMBL
Match: A0A067FWY2_CITSI (Uncharacterized protein OS=Citrus sinensis GN=CISIN_1g020053mg PE=4 SV=1)

HSP 1 Score: 447.6 bits (1150), Expect = 1.2e-122
Identity = 232/306 (75.82%), Postives = 254/306 (83.01%), Query Frame = 1 

Query: 1   MAEDKYNLKNPAVKRILQEVKEMQANPSDDLMSLPLEENIFEWQFAIRGPGDSEFEGGIY 60
           MAED+YNLKNPAVKRILQEVKEMQ+NPSDD MSLPLEENIFEWQFAIRGPGD+EFEGGIY
Sbjct: 1   MAEDRYNLKNPAVKRILQEVKEMQSNPSDDFMSLPLEENIFEWQFAIRGPGDTEFEGGIY 60

Query: 61  HGRIQLPAEYPFKPPSFMLLTPNGRFETQTKICLSISNHHPEHWQPSWSVRTALVALIAF 120
           HGRIQLPAEYPFKPPSFMLLTPNGRFETQTKICLSISNHHPEHWQPSWSVRTALVALIAF
Sbjct: 61  HGRIQLPAEYPFKPPSFMLLTPNGRFETQTKICLSISNHHPEHWQPSWSVRTALVALIAF 120

Query: 121 MPTNPNGALGSLDYKKEERRVLAIKSREAPPKFGTPERQKLIDEIHEYMLSKAPPVPQSG 180
           MPTNPNGALGSLDYKKEERR LAIKSREA PKFGTPERQKLIDEIHEYMLSKAPPVPQ  
Sbjct: 121 MPTNPNGALGSLDYKKEERRALAIKSREAAPKFGTPERQKLIDEIHEYMLSKAPPVPQLS 180

Query: 181 STDVSGEKPTNEEADVQVTSETAELAAAVEELQNSPTINRIGEEAVPEV------QPEVA 240
           + +   E+P N E + Q +S  A +  A E L  +P  +RI EE   ++       P VA
Sbjct: 181 TCE---EQPGNREGEAQASSIDAMVTGAGEGLP-APVGDRIIEEVQEDLPASMNPNPVVA 240

Query: 241 ---RVAPSSRPTTQTEQGRPETRVHKPADDRLFTWAAIGLTLAIVVLLLKKFMKASAHGS 298
              R  P++    Q  Q +PETRV KPADDRLFTWAA+GLT+AI+VLLLKKFMK++ HG+
Sbjct: 241 GTSREVPANASGVQL-QPKPETRVQKPADDRLFTWAAVGLTIAILVLLLKKFMKSNGHGT 300

[Close]
BLAST of Cucsa.252590 vs. TrEMBL
Match: W9RG08_9ROSA (Ubiquitin-conjugating enzyme E2 32 OS=Morus notabilis GN=L484_004205 PE=4 SV=1)

HSP 1 Score: 444.5 bits (1142), Expect = 1.0e-121
Identity = 225/302 (74.50%), Postives = 247/302 (81.79%), Query Frame = 1 

Query: 1   MAEDKYNLKNPAVKRILQEVKEMQANPSDDLMSLPLEENIFEWQFAIRGPGDSEFEGGIY 60
           MAEDKYNLKNPAVKRILQE+KEMQ+N SDD MSLPLEENIFEWQFAIRGP D+EFEGGIY
Sbjct: 1   MAEDKYNLKNPAVKRILQEIKEMQSNSSDDFMSLPLEENIFEWQFAIRGPRDTEFEGGIY 60

Query: 61  HGRIQLPAEYPFKPPSFMLLTPNGRFETQTKICLSISNHHPEHWQPSWSVRTALVALIAF 120
           HGRIQLPAEYPFKPPSFMLLTPNGRFETQTKICLSISNHHPEHWQPSWSVRTALVALIAF
Sbjct: 61  HGRIQLPAEYPFKPPSFMLLTPNGRFETQTKICLSISNHHPEHWQPSWSVRTALVALIAF 120

Query: 121 MPTNPNGALGSLDYKKEERRVLAIKSREAPPKFGTPERQKLIDEIHEYMLSKAPPVPQSG 180
           MPT+PNGALGSLDYKKEERR LA+KSREA PKFGTPERQKLIDE+HEYMLSKAPPVPQ  
Sbjct: 121 MPTSPNGALGSLDYKKEERRALAVKSREASPKFGTPERQKLIDEVHEYMLSKAPPVPQLC 180

Query: 181 STDVSGEKPTN-EEADVQVTSETAELAAAVEELQNSPTINRIGE---EAVPEVQPEVARV 240
            ++   E+PT  EE D Q  S+ +++A A E L N     R+ E   E+     P +AR 
Sbjct: 181 PSETPEEQPTKIEEGDAQAGSQNSDVAQAAEGLPNPSVGERVVEEHQESPANANPRLARE 240

Query: 241 -APSSRPTTQTEQGRPETRVHKPADDRLFTWAAIGLTLAIVVLLLKKFMKASAHGSVFMD 298
            APS   ++     R E R  +PADDRLFTWAA+GLT+AIVVLLLKKFM A  HG+VFMD
Sbjct: 241 RAPSEVHSSNQVLQRQEVRAQRPADDRLFTWAAVGLTIAIVVLLLKKFMNAGGHGAVFMD 300

[Close]
BLAST of Cucsa.252590 vs. TrEMBL
Match: I1MGF5_SOYBN (Uncharacterized protein OS=Glycine max GN=GLYMA_15G141200 PE=4 SV=1)

HSP 1 Score: 443.7 bits (1140), Expect = 1.7e-121
Identity = 228/305 (74.75%), Postives = 245/305 (80.33%), Query Frame = 1 

Query: 4   DKYNLKNPAVKRILQEVKEMQANPSDDLMSLPLEENIFEWQFAIRGPGDSEFEGGIYHGR 63
           DKYNLKNPAVKRILQEVKEMQ+NPSDD MSLPLEENIFEWQFAIRGP D+EFEGGIYHGR
Sbjct: 3   DKYNLKNPAVKRILQEVKEMQSNPSDDFMSLPLEENIFEWQFAIRGPRDTEFEGGIYHGR 62

Query: 64  IQLPAEYPFKPPSFMLLTPNGRFETQTKICLSISNHHPEHWQPSWSVRTALVALIAFMPT 123
           IQLP+EYPFKPPSFMLLTPNGRFETQTKICLSISNHHPEHWQPSWSVRTALVALIAFMPT
Sbjct: 63  IQLPSEYPFKPPSFMLLTPNGRFETQTKICLSISNHHPEHWQPSWSVRTALVALIAFMPT 122

Query: 124 NPNGALGSLDYKKEERRVLAIKSREAPPKFGTPERQKLIDEIHEYMLSKAPPVPQSGSTD 183
           NPNGALGSLDYKKEER  LAIKSREAPPKFGTPERQKLIDEIHEYMLSKAPPVPQ  +T+
Sbjct: 123 NPNGALGSLDYKKEERHTLAIKSREAPPKFGTPERQKLIDEIHEYMLSKAPPVPQPSATE 182

Query: 184 VSGEKPTNEEADVQVTSETAELAAAVE-----------ELQNSPTINRIGEEAVPEVQPE 243
            S E P NEEA+ QV S   E   A E           E Q     N  G E   EVQP 
Sbjct: 183 ASEENPRNEEAETQVNSPNPESLPAGEGITDQEVDGIVEEQEVILANPAGVEVSREVQPS 242

Query: 244 VARVAPSSRPTTQTEQGRPETRVHKPADDRLFTWAAIGLTLAIVVLLLKKFMKASAHGSV 298
           V+R     +  T+ +  +PETRV K  DDRLFT AAIGLT+AIVVLLLKKF+K++ HG++
Sbjct: 243 VSRNEVLQKSDTRAQNPKPETRVQK-HDDRLFTLAAIGLTIAIVVLLLKKFLKSTQHGAL 302

[Close]
BLAST of Cucsa.252590 vs. TAIR10
Match: AT3G17000.1 (AT3G17000.1 ubiquitin-conjugating enzyme 32)

HSP 1 Score: 393.7 bits (1010), Expect = 1.0e-109
Identity = 201/309 (65.05%), Postives = 234/309 (75.73%), Query Frame = 1 

Query: 1   MAEDKYNLKNPAVKRILQEVKEMQANPSDDLMSLPLEENIFEWQFAIRGPGDSEFEGGIY 60
           MA+++YN KNPAVKRILQEVKEMQANPSDD MSLPLEENIFEWQFAIRGPGD+EFEGGIY
Sbjct: 1   MADERYNRKNPAVKRILQEVKEMQANPSDDFMSLPLEENIFEWQFAIRGPGDTEFEGGIY 60

Query: 61  HGRIQLPAEYPFKPPSFMLLTPNGRFETQTKICLSISNHHPEHWQPSWSVRTALVALIAF 120
           HGRIQLPA+YPFKPPSFMLLTPNGRFET TKICLSISN+HPEHWQPSWSVRTALVALIAF
Sbjct: 61  HGRIQLPADYPFKPPSFMLLTPNGRFETNTKICLSISNYHPEHWQPSWSVRTALVALIAF 120

Query: 121 MPTNPNGALGSLDYKKEERRVLAIKSREAPPKFGTPERQKLIDEIHEYMLSKAPPVPQSG 180
           MPT+PNGALGS+DY K+ERR LAIKSRE PPK+G+PERQK+IDEIH+Y+LSKA  VP+  
Sbjct: 121 MPTSPNGALGSVDYPKDERRTLAIKSRETPPKYGSPERQKIIDEIHQYILSKATVVPKPL 180

Query: 181 STDVSGEKPTNEEADVQVTSETAELAAAVEELQNSPTINRIGEEAVPEVQPEV---ARVA 240
             + S       EA  QV  E  E    VEE   + T   + ++ + E    V   A V 
Sbjct: 181 PLECSQAPSIVSEAHSQV--EPQEAITVVEERSIATTDTIVDDQIIEETAEAVNTAASVV 240

Query: 241 PSSRP----------TTQTEQGRPETRVHKPADDRLFTWAAIGLTLAIVVLLLKKFMKAS 297
           P++ P          +   EQ        KP DDRLFTWAA+GLT+AI+VLLLKKF+K++
Sbjct: 241 PAAAPLPAVEVVVKASVSGEQRMARRAAQKPVDDRLFTWAAVGLTIAIMVLLLKKFIKSN 300

[Close]
BLAST of Cucsa.252590 vs. TAIR10
Match: AT1G17280.1 (AT1G17280.1 ubiquitin-conjugating enzyme 34)

HSP 1 Score: 123.2 bits (308), Expect = 2.6e-28
Identity = 71/198 (35.86%), Postives = 109/198 (55.05%), Query Frame = 1 

Query: 13  VKRILQEVKEMQANPSDDLMSLPLEENIFEWQFAIRGPGDSEFEGGIYHGRIQLPAEYPF 72
           +KR+ +E + +   P   +++ P   +I EW + + G   + F GG Y+G+I+ P EYP+
Sbjct: 7   IKRLQKEYRALCKEPVSHVVARPSPNDILEWHYVLEGSEGTPFAGGFYYGKIKFPPEYPY 66

Query: 73  KPPSFMLLTPNGRFETQTKICLSISNHHPEHWQPSWSVRTALVALIAF-MPTNPNGALGS 132
           KPP   + TPNGRF TQ KICLS+S+ HPE W P WSV + L  L++F M T+P    GS
Sbjct: 67  KPPGITMTTPNGRFMTQKKICLSMSDFHPESWNPMWSVSSILTGLLSFMMDTSP--TTGS 126

Query: 133 LDYKKEERRVLAIKSREAPPKFGTPERQKL----IDEIHEYMLSK-------APPVPQSG 192
           ++    E++ LA  S     K  TP  +KL    +++ ++  L++        P  PQ  
Sbjct: 127 VNTTVIEKQRLAKSSLAFNCK--TPAFRKLFPEYVEKYNQQQLAEQATTQLTTPESPQKS 186

Query: 193 STDVSGEK---PTNEEAD 196
            T V  EK   PT  +++
Sbjct: 187 DTKVESEKTIDPTKGDSE 200

[Close]
BLAST of Cucsa.252590 vs. TAIR10
Match: AT5G50430.1 (AT5G50430.1 ubiquitin-conjugating enzyme 33)

HSP 1 Score: 122.5 bits (306), Expect = 4.5e-28
Identity = 63/202 (31.19%), Postives = 108/202 (53.47%), Query Frame = 1 

Query: 13  VKRILQEVKEMQANPSDDLMSLPLEENIFEWQFAIRGPGDSEFEGGIYHGRIQLPAEYPF 72
           +KR+ +E + +   P   +++ P   +I EW + + G   + F GG Y+G+I+ P EYP+
Sbjct: 7   IKRLQKEYRALCKEPVSHVVARPSPNDILEWHYVLEGSEGTPFAGGFYYGKIKFPPEYPY 66

Query: 73  KPPSFMLLTPNGRFETQTKICLSISNHHPEHWQPSWSVRTALVALIAFMPTNPNGALGSL 132
           KPP   + TPNGRF TQ KICLS+S+ HPE W P WSV + L  L++FM  N +   GS+
Sbjct: 67  KPPGITMTTPNGRFVTQKKICLSMSDFHPESWNPMWSVSSILTGLLSFMMDN-SPTTGSV 126

Query: 133 DYKKEERRVLAIKSREAPPKFGTPER--QKLIDEIHEYMLSKAPPVPQSGSTDVSGEKPT 192
           +    E++ LA  S     K  T  +   + +++  +  +++     Q  +T  + + P 
Sbjct: 127 NTSVAEKQRLAKSSLAFNCKSVTFRKLFPEYVEKYSQQQVAEEEAATQQTTTSENQDFPQ 186

Query: 193 NEEADVQVTSETAELAAAVEEL 213
            + A V+          +++E+
Sbjct: 187 KDNAKVESEKSVGLKKESIQEV 207

[Close]
BLAST of Cucsa.252590 vs. TAIR10
Match: AT4G27960.2 (AT4G27960.2 ubiquitin conjugating enzyme 9)

HSP 1 Score: 80.1 bits (196), Expect = 2.5e-15
Identity = 41/117 (35.04%), Postives = 65/117 (55.56%), Query Frame = 1 

Query: 12  AVKRILQEVKEMQANPSDDLMSLPLEENIFEWQFAIRGPGDSEFEGGIYHGRIQLPAEYP 71
           A KRIL+E+K++Q +P     + P+ E++F WQ  I GP DS + GG++   I  P +YP
Sbjct: 32  ASKRILKELKDLQKDPPTSCSAGPVAEDMFHWQATIMGPSDSPYSGGVFLVTIHFPPDYP 91

Query: 72  FKPPSFMLLTP--NGRFETQTKICLSISNHHPEHWQPSWSVRTALVALIAFMPTNPN 127
           FKPP     T   +    +   ICL I     E W P+ ++   L+++ + + T+PN
Sbjct: 92  FKPPKVAFRTKVFHPNINSNGSICLDILK---EQWSPALTISKVLLSICSLL-TDPN 144

[Close]
BLAST of Cucsa.252590 vs. TAIR10
Match: AT3G08690.1 (AT3G08690.1 ubiquitin-conjugating enzyme 11)

HSP 1 Score: 79.3 bits (194), Expect = 4.3e-15
Identity = 41/117 (35.04%), Postives = 67/117 (57.26%), Query Frame = 1 

Query: 12  AVKRILQEVKEMQANPSDDLMSLPLEENIFEWQFAIRGPGDSEFEGGIYHGRIQLPAEYP 71
           A KRIL+E+K++Q +P  +  + P+ E++F WQ  I GP +S + GG++   I  P +YP
Sbjct: 2   ASKRILKELKDLQKDPPSNCSAGPVAEDMFHWQATIMGPPESPYAGGVFLVSIHFPPDYP 61

Query: 72  FKPP--SFMLLTPNGRFETQTKICLSISNHHPEHWQPSWSVRTALVALIAFMPTNPN 127
           FKPP  SF     +    +   ICL I     E W P+ ++   L+++ + + T+PN
Sbjct: 62  FKPPKVSFKTKVYHPNINSNGSICLDILK---EQWSPALTISKVLLSICSLL-TDPN 114

[Close]
BLAST of Cucsa.252590 vs. NCBI nr
Match: gi|449432914|ref|XP_004134243.1| (PREDICTED: ubiquitin-conjugating enzyme E2 32 [Cucumis sativus])

HSP 1 Score: 593.6 bits (1529), Expect = 1.9e-166
Identity = 295/297 (99.33%), Postives = 295/297 (99.33%), Query Frame = 1 

Query: 1   MAEDKYNLKNPAVKRILQEVKEMQANPSDDLMSLPLEENIFEWQFAIRGPGDSEFEGGIY 60
           MAEDKYNLKNPAVKRILQEVKEMQANPSDD MSLPLEENIFEWQFAIRGPGDSEFEGGIY
Sbjct: 1   MAEDKYNLKNPAVKRILQEVKEMQANPSDDFMSLPLEENIFEWQFAIRGPGDSEFEGGIY 60

Query: 61  HGRIQLPAEYPFKPPSFMLLTPNGRFETQTKICLSISNHHPEHWQPSWSVRTALVALIAF 120
           HGRIQLPAEYPFKPPSFMLLTPNGRFETQTKICLSISNHHPEHWQPSWSVRTALVALIAF
Sbjct: 61  HGRIQLPAEYPFKPPSFMLLTPNGRFETQTKICLSISNHHPEHWQPSWSVRTALVALIAF 120

Query: 121 MPTNPNGALGSLDYKKEERRVLAIKSREAPPKFGTPERQKLIDEIHEYMLSKAPPVPQSG 180
           MPTNPNGALGSLDYKKEERRVLAIKSREAPPKFGTPERQKLIDEIHEYMLSKAPPVPQSG
Sbjct: 121 MPTNPNGALGSLDYKKEERRVLAIKSREAPPKFGTPERQKLIDEIHEYMLSKAPPVPQSG 180

Query: 181 STDVSGEKPTNEEADVQVTSETAELAAAVEELQNSPTINRIGEEAVPEVQPEVARVAPSS 240
           STDVSGEKPTNEEADVQVTSETAELAAAVEELQNSPTINRI EEAVPEVQPEVARVAPSS
Sbjct: 181 STDVSGEKPTNEEADVQVTSETAELAAAVEELQNSPTINRIVEEAVPEVQPEVARVAPSS 240

Query: 241 RPTTQTEQGRPETRVHKPADDRLFTWAAIGLTLAIVVLLLKKFMKASAHGSVFMDES 298
           RPTTQTEQGRPETRVHKPADDRLFTWAAIGLTLAIVVLLLKKFMKASAHGSVFMDES
Sbjct: 241 RPTTQTEQGRPETRVHKPADDRLFTWAAIGLTLAIVVLLLKKFMKASAHGSVFMDES 297

[Close]
BLAST of Cucsa.252590 vs. NCBI nr
Match: gi|659076938|ref|XP_008438945.1| (PREDICTED: ubiquitin-conjugating enzyme E2 32 [Cucumis melo])

HSP 1 Score: 571.6 bits (1472), Expect = 7.9e-160
Identity = 284/297 (95.62%), Postives = 288/297 (96.97%), Query Frame = 1 

Query: 1   MAEDKYNLKNPAVKRILQEVKEMQANPSDDLMSLPLEENIFEWQFAIRGPGDSEFEGGIY 60
           MAEDKYN+KNPAVKRILQEVKEMQANPSDD MSLPLEENIFEWQFAIRGP DSEFEGGIY
Sbjct: 1   MAEDKYNMKNPAVKRILQEVKEMQANPSDDFMSLPLEENIFEWQFAIRGPDDSEFEGGIY 60

Query: 61  HGRIQLPAEYPFKPPSFMLLTPNGRFETQTKICLSISNHHPEHWQPSWSVRTALVALIAF 120
           HGRIQLPAEYPFKPPSFMLLTPNGRFETQTKICLSISNHHPEHWQPSWSVRTALVALIAF
Sbjct: 61  HGRIQLPAEYPFKPPSFMLLTPNGRFETQTKICLSISNHHPEHWQPSWSVRTALVALIAF 120

Query: 121 MPTNPNGALGSLDYKKEERRVLAIKSREAPPKFGTPERQKLIDEIHEYMLSKAPPVPQSG 180
           MPTNPNGALGSLDYKKEERRVLAIKSREAPPKFGTPERQKLIDEIHEYMLSKAPPVPQSG
Sbjct: 121 MPTNPNGALGSLDYKKEERRVLAIKSREAPPKFGTPERQKLIDEIHEYMLSKAPPVPQSG 180

Query: 181 STDVSGEKPTNEEADVQVTSETAELAAAVEELQNSPTINRIGEEAVPEVQPEVARVAPSS 240
           S+DVS +KPTNEE+DVQVTSE AELAAA EELQN PTINRI EEAVPEVQPEVARVAPSS
Sbjct: 181 SSDVSDQKPTNEESDVQVTSEPAELAAAGEELQNPPTINRIVEEAVPEVQPEVARVAPSS 240

Query: 241 RPTTQTEQGRPETRVHKPADDRLFTWAAIGLTLAIVVLLLKKFMKASAHGSVFMDES 298
           RPTTQ EQ RPETRVHKPADDRLFTWAAIGLTLAIVVLLLKKFMKASAHGSVFMDES
Sbjct: 241 RPTTQAEQQRPETRVHKPADDRLFTWAAIGLTLAIVVLLLKKFMKASAHGSVFMDES 297

[Close]
BLAST of Cucsa.252590 vs. NCBI nr
Match: gi|567852467|ref|XP_006419397.1| (hypothetical protein CICLE_v10005518mg [Citrus clementina])

HSP 1 Score: 449.1 bits (1154), Expect = 5.9e-123
Identity = 233/306 (76.14%), Postives = 255/306 (83.33%), Query Frame = 1 

Query: 1   MAEDKYNLKNPAVKRILQEVKEMQANPSDDLMSLPLEENIFEWQFAIRGPGDSEFEGGIY 60
           MAED+YNLKNPAVKRILQEVKEMQ+NPSDD MSLPLEENIFEWQFAIRGPGD+EFEGGIY
Sbjct: 1   MAEDRYNLKNPAVKRILQEVKEMQSNPSDDFMSLPLEENIFEWQFAIRGPGDTEFEGGIY 60

Query: 61  HGRIQLPAEYPFKPPSFMLLTPNGRFETQTKICLSISNHHPEHWQPSWSVRTALVALIAF 120
           HGRIQLPAEYPFKPPSFMLLTPNGRFETQTKICLSISNHHPEHWQPSWSVRTALVALIAF
Sbjct: 61  HGRIQLPAEYPFKPPSFMLLTPNGRFETQTKICLSISNHHPEHWQPSWSVRTALVALIAF 120

Query: 121 MPTNPNGALGSLDYKKEERRVLAIKSREAPPKFGTPERQKLIDEIHEYMLSKAPPVPQSG 180
           MPTNPNGALGSLDYKKEERR LAIKSREA PKFGTPERQKLIDEIHEYMLSKAPPVPQ  
Sbjct: 121 MPTNPNGALGSLDYKKEERRALAIKSREAAPKFGTPERQKLIDEIHEYMLSKAPPVPQLS 180

Query: 181 STDVSGEKPTNEEADVQVTSETAELAAAVEELQNSPTINRIGEEAVPEV------QPEVA 240
           + +   E+P N E + Q +S  A +A A E L  +P  +RI EE   ++       P VA
Sbjct: 181 TCE---EQPGNREGEAQASSIDAMVAGAGEGLP-APVGDRIIEEVQEDLPASMNPNPVVA 240

Query: 241 ---RVAPSSRPTTQTEQGRPETRVHKPADDRLFTWAAIGLTLAIVVLLLKKFMKASAHGS 298
              R  P++    Q  Q +PETRV KPADDRLFTWAA+GLT+AI+VLLLKKFMK++ HG+
Sbjct: 241 GTSREVPANASGVQL-QPKPETRVQKPADDRLFTWAAVGLTIAILVLLLKKFMKSNGHGA 300

[Close]
BLAST of Cucsa.252590 vs. NCBI nr
Match: gi|568871343|ref|XP_006488848.1| (PREDICTED: ubiquitin-conjugating enzyme E2 32 [Citrus sinensis])

HSP 1 Score: 447.6 bits (1150), Expect = 1.7e-122
Identity = 232/306 (75.82%), Postives = 254/306 (83.01%), Query Frame = 1 

Query: 1   MAEDKYNLKNPAVKRILQEVKEMQANPSDDLMSLPLEENIFEWQFAIRGPGDSEFEGGIY 60
           MAED+YNLKNPAVKRILQEVKEMQ+NPSDD MSLPLEENIFEWQFAIRGPGD+EFEGGIY
Sbjct: 1   MAEDRYNLKNPAVKRILQEVKEMQSNPSDDFMSLPLEENIFEWQFAIRGPGDTEFEGGIY 60

Query: 61  HGRIQLPAEYPFKPPSFMLLTPNGRFETQTKICLSISNHHPEHWQPSWSVRTALVALIAF 120
           HGRIQLPAEYPFKPPSFMLLTPNGRFETQTKICLSISNHHPEHWQPSWSVRTALVALIAF
Sbjct: 61  HGRIQLPAEYPFKPPSFMLLTPNGRFETQTKICLSISNHHPEHWQPSWSVRTALVALIAF 120

Query: 121 MPTNPNGALGSLDYKKEERRVLAIKSREAPPKFGTPERQKLIDEIHEYMLSKAPPVPQSG 180
           MPTNPNGALGSLDYKKEERR LAIKSREA PKFGTPERQKLIDEIHEYMLSKAPPVPQ  
Sbjct: 121 MPTNPNGALGSLDYKKEERRALAIKSREAAPKFGTPERQKLIDEIHEYMLSKAPPVPQLS 180

Query: 181 STDVSGEKPTNEEADVQVTSETAELAAAVEELQNSPTINRIGEEAVPEV------QPEVA 240
           + +   E+P N E + Q +S  A +  A E L  +P  +RI EE   ++       P VA
Sbjct: 181 TCE---EQPGNREGEAQASSIDAMVTGAGEGLP-APVGDRIIEEVQEDLPASMNPNPVVA 240

Query: 241 ---RVAPSSRPTTQTEQGRPETRVHKPADDRLFTWAAIGLTLAIVVLLLKKFMKASAHGS 298
              R  P++    Q  Q +PETRV KPADDRLFTWAA+GLT+AI+VLLLKKFMK++ HG+
Sbjct: 241 GTSREVPANASGVQL-QPKPETRVQKPADDRLFTWAAVGLTIAILVLLLKKFMKSNGHGT 300

[Close]
BLAST of Cucsa.252590 vs. NCBI nr
Match: gi|1009132113|ref|XP_015883210.1| (PREDICTED: ubiquitin-conjugating enzyme E2 32 [Ziziphus jujuba])

HSP 1 Score: 445.7 bits (1145), Expect = 6.5e-122
Identity = 230/301 (76.41%), Postives = 242/301 (80.40%), Query Frame = 1 

Query: 1   MAEDKYNLKNPAVKRILQEVKEMQANPSDDLMSLPLEENIFEWQFAIRGPGDSEFEGGIY 60
           MA+DKYNLKNPAVKRILQEVKEMQ+NPSDD MSLPLEENIFEWQFAIRGP DSEFEGGIY
Sbjct: 1   MADDKYNLKNPAVKRILQEVKEMQSNPSDDFMSLPLEENIFEWQFAIRGPRDSEFEGGIY 60

Query: 61  HGRIQLPAEYPFKPPSFMLLTPNGRFETQTKICLSISNHHPEHWQPSWSVRTALVALIAF 120
           HGRIQLPAEYPFKPPSFMLLTPNGRFETQTKICLSISNHHPEHWQPSWSVRTALVALIAF
Sbjct: 61  HGRIQLPAEYPFKPPSFMLLTPNGRFETQTKICLSISNHHPEHWQPSWSVRTALVALIAF 120

Query: 121 MPTNPNGALGSLDYKKEERRVLAIKSREAPPKFGTPERQKLIDEIHEYMLSKAPPVPQSG 180
           MPTNPNGALGSLDYKKEERR LAIKSRE PPKFGTPERQKLIDEIHEYMLSKAPPVPQS 
Sbjct: 121 MPTNPNGALGSLDYKKEERRALAIKSRETPPKFGTPERQKLIDEIHEYMLSKAPPVPQSS 180

Query: 181 STDVSGEKPTNEEADVQVTSETAELAAAVEELQNSPTINRIGEEAV-PEVQPEVARV--- 240
            +    E     E + Q +S     A A E L N P  + I E  +   V P   RV   
Sbjct: 181 PSQALEENSPKSEDEAQKSSVDDGAAPASESLPNPPAGDVIVERVLEAPVNPNPGRVSVR 240

Query: 241 APSSRPTTQTEQGRPETRVHKPADDRLFTWAAIGLTLAIVVLLLKKFMKASAHGSVFMDE 298
           A    P T T     +TRV KPADDRLFTWAA+GLTLAI+VLLLKKFMK+S  G+VFMD 
Sbjct: 241 AVREVPPTSTSNQMLQTRVQKPADDRLFTWAAVGLTLAILVLLLKKFMKSSGPGAVFMDG 300

The following BLAST results are available for this feature:
BLAST of Cucsa.252590 vs. Swiss-Prot
Match NameE-valueIdentityDescription
UBC32_ARATH1.8e-10865.05Ubiquitin-conjugating enzyme E2 32 OS=Arabidopsis thaliana GN=UBC32 PE=2 SV=1[more]
UB2J1_MOUSE1.9e-5241.32Ubiquitin-conjugating enzyme E2 J1 OS=Mus musculus GN=Ube2j1 PE=1 SV=2[more]
UB2J1_HUMAN4.2e-5264.38Ubiquitin-conjugating enzyme E2 J1 OS=Homo sapiens GN=UBE2J1 PE=1 SV=2[more]
UBC6_CANGA3.7e-3245.19Ubiquitin-conjugating enzyme E2 6 OS=Candida glabrata (strain ATCC 2001 / CBS 13... [more]
UBC6_KLULA2.0e-3033.49Ubiquitin-conjugating enzyme E2 6 OS=Kluyveromyces lactis (strain ATCC 8585 / CB... [more]
BLAST of Cucsa.252590 vs. TrEMBL
Match NameE-valueIdentityDescription
A0A0A0L954_CUCSA1.4e-16699.33Uncharacterized protein OS=Cucumis sativus GN=Csa_3G166270 PE=4 SV=1[more]
V4RY15_9ROSI4.1e-12376.14Uncharacterized protein OS=Citrus clementina GN=CICLE_v10005518mg PE=4 SV=1[more]
A0A067FWY2_CITSI1.2e-12275.82Uncharacterized protein OS=Citrus sinensis GN=CISIN_1g020053mg PE=4 SV=1[more]
W9RG08_9ROSA1.0e-12174.50Ubiquitin-conjugating enzyme E2 32 OS=Morus notabilis GN=L484_004205 PE=4 SV=1[more]
I1MGF5_SOYBN1.7e-12174.75Uncharacterized protein OS=Glycine max GN=GLYMA_15G141200 PE=4 SV=1[more]
BLAST of Cucsa.252590 vs. TAIR10
Match NameE-valueIdentityDescription
AT3G17000.11.0e-10965.05 ubiquitin-conjugating enzyme 32[more]
AT1G17280.12.6e-2835.86 ubiquitin-conjugating enzyme 34[more]
AT5G50430.14.5e-2831.19 ubiquitin-conjugating enzyme 33[more]
AT4G27960.22.5e-1535.04 ubiquitin conjugating enzyme 9[more]
AT3G08690.14.3e-1535.04 ubiquitin-conjugating enzyme 11[more]
BLAST of Cucsa.252590 vs. NCBI nr
Match NameE-valueIdentityDescription
gi|449432914|ref|XP_004134243.1|1.9e-16699.33PREDICTED: ubiquitin-conjugating enzyme E2 32 [Cucumis sativus][more]
gi|659076938|ref|XP_008438945.1|7.9e-16095.62PREDICTED: ubiquitin-conjugating enzyme E2 32 [Cucumis melo][more]
gi|567852467|ref|XP_006419397.1|5.9e-12376.14hypothetical protein CICLE_v10005518mg [Citrus clementina][more]
gi|568871343|ref|XP_006488848.1|1.7e-12275.82PREDICTED: ubiquitin-conjugating enzyme E2 32 [Citrus sinensis][more]
gi|1009132113|ref|XP_015883210.1|6.5e-12276.41PREDICTED: ubiquitin-conjugating enzyme E2 32 [Ziziphus jujuba][more]
Annotated Terms
The following terms have been associated with this gene:
Vocabulary: INTERPRO
TermDefinition
IPR000608UBQ-conjugat_E2
IPR016135UBQ-conjugating_enzyme/RWD
GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0016036 cellular response to phosphate starvation
biological_process GO:0042631 cellular response to water deprivation
biological_process GO:0006635 fatty acid beta-oxidation
biological_process GO:0019375 galactolipid biosynthetic process
biological_process GO:0045892 negative regulation of transcription, DNA-templated
biological_process GO:0006301 postreplication repair
biological_process GO:0080129 proteasome core complex assembly
biological_process GO:0043161 proteasome-mediated ubiquitin-dependent protein catabolic process
biological_process GO:0051788 response to misfolded protein
biological_process GO:0009407 toxin catabolic process
biological_process GO:0008150 biological_process
cellular_component GO:0005783 endoplasmic reticulum
cellular_component GO:0048471 perinuclear region of cytoplasm
cellular_component GO:0005575 cellular_component
molecular_function GO:0003674 molecular_function
Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Cucsa.252590.1Cucsa.252590.1mRNA


InterPro
Analysis Name: InterPro Annotations of cucumber (Gy14)
Date Performed: 2017-01-17
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR000608Ubiquitin-conjugating enzyme E2PFAMPF00179UQ_concoord: 15..126
score: 1.5
IPR000608Ubiquitin-conjugating enzyme E2PROFILEPS50127UBIQUITIN_CONJUGAT_2coord: 14..126
score: 23
IPR016135Ubiquitin-conjugating enzyme/RWD-likeGENE3DG3DSA:3.10.110.10coord: 7..197
score: 1.9
IPR016135Ubiquitin-conjugating enzyme/RWD-likeunknownSSF54495UBC-likecoord: 12..134
score: 5.38
NoneNo IPR availablePANTHERPTHR24067UBIQUITIN-CONJUGATING ENZYME E2coord: 1..148
score: 6.8
NoneNo IPR availablePANTHERPTHR24067:SF54UBIQUITIN-CONJUGATING ENZYME E2 J1coord: 1..148
score: 6.8
NoneNo IPR availableSMARTSM00212ubc_7coord: 14..174
score: 3.2

Synteny
The following gene(s) are orthologous to this gene:
GeneOrthologueOrganismBlock
Cucsa.252590Cla021788Watermelon (97103) v1cgywmB479
Cucsa.252590Csa3G166270Cucumber (Chinese Long) v2cgycuB366
Cucsa.252590MELO3C006710Melon (DHL92) v3.5.1cgymeB439
Cucsa.252590ClCG05G006510Watermelon (Charleston Gray)cgywcgB451
Cucsa.252590CSPI03G14930Wild cucumber (PI 183967)cgycpiB383
Cucsa.252590CmaCh14G015930Cucurbita maxima (Rimu)cgycmaB0709
Cucsa.252590CmaCh06G015600Cucurbita maxima (Rimu)cgycmaB0727
Cucsa.252590CmoCh14G016240Cucurbita moschata (Rifu)cgycmoB0700
Cucsa.252590CmoCh06G015570Cucurbita moschata (Rifu)cgycmoB0718
Cucsa.252590Lsi05G015870Bottle gourd (USVL1VR-Ls)cgylsiB420
Cucsa.252590Cp4.1LG03g11700Cucurbita pepo (Zucchini)cgycpeB0682
Cucsa.252590Cp4.1LG08g05100Cucurbita pepo (Zucchini)cgycpeB0687
Cucsa.252590MELO3C006710.2Melon (DHL92) v3.6.1cgymedB432
Cucsa.252590CsaV3_3G015120Cucumber (Chinese Long) v3cgycucB387
Cucsa.252590Cla97C05G088110Watermelon (97103) v2cgywmbB452
Cucsa.252590Bhi01G000775Wax gourdcgywgoB522
Cucsa.252590Carg19627Silver-seed gourdcarcgyB0329
Cucsa.252590Carg23738Silver-seed gourdcarcgyB1072
The following gene(s) are paralogous to this gene:

None

The following block(s) are covering this gene:
GeneOrganismBlock
Cucsa.252590Melon (DHL92) v3.6.1cgymedB425
Cucsa.252590Melon (DHL92) v3.6.1cgymedB427
Cucsa.252590Silver-seed gourdcarcgyB0083
Cucsa.252590Silver-seed gourdcarcgyB0788
Cucsa.252590Watermelon (97103) v2cgywmbB453
Cucsa.252590Wax gourdcgywgoB512
Cucsa.252590Cucurbita maxima (Rimu)cgycmaB0714
Cucsa.252590Cucurbita maxima (Rimu)cgycmaB0731
Cucsa.252590Cucurbita moschata (Rifu)cgycmoB0707
Cucsa.252590Cucurbita moschata (Rifu)cgycmoB0720
Cucsa.252590Melon (DHL92) v3.5.1cgymeB430
Cucsa.252590Watermelon (Charleston Gray)cgywcgB453
Cucsa.252590Watermelon (97103) v1cgywmB483
Cucsa.252590Cucurbita pepo (Zucchini)cgycpeB0671
Cucsa.252590Cucurbita pepo (Zucchini)cgycpeB0678
Cucsa.252590Bottle gourd (USVL1VR-Ls)cgylsiB412