Cp4.1LG08g05100 (gene) Cucurbita pepo (Zucchini)

Overview
NameCp4.1LG08g05100
Typegene
OrganismCucurbita pepo (Cucurbita pepo (Zucchini))
DescriptionUbiquitin-conjugating enzyme, E2
LocationCp4.1LG08 : 1002181 .. 1004905 (-)
Genome Viewer
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: five_prime_UTRCDSthree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
AAAATGACGTGGCGTGTTAATAGCCGACTTTTGTTCTTTCGCTCCCCTTCCTCCGTGCCCATAAATTGCTGTTCCCGTTCAATTTCTACGGATTGGCTTTGATTTCGATTCAAATCCTCTCGTCTGGTTTTAGGTTGAGTTTTTTTTTTTTCACCGTTCTCGATCGGATAATTTTTCGTCTCGTGATCGGGTGGCGGTTGCAGTCGGATCGGATCTGCGAGTTATTTGAAAAGATATGGCGGAGGATAAGTACAATTTGAAGAACCCTGCGGTGAAGAGGATCCTTCGGGAGGTCAAGGAGATGCAGGCTAATCCTTCTGATGATTTCATGAGTCTGCCTCTCGAGGTTCGTTGCTTTTGAATTTCCCTGGATTTCCTTTTCCATTAATTATTTCTTTTTCTTTTTTTTTCCTTGAAAACTNTAATGGTTTTTCGTATTTTTGTTGAATGAATTGATTCACTATGATTGTTTCCGACTATTCAAATGATCTGATACGCGTGGAATTGTGGTTGAAATTAAGTATTAATTTGACGTTATTTTATAGTTCAAATCTGCTGGTGTCTGCCGTTGCTTGCATATGAATTTCTAGGGTTCTAAGGATGAAAATGGAAATATTAACGAGTGTTTGTGGATTTCATTCCAGTTTTGTATGTTTGGAATTTTTTTGGACAGCTGAAAATTGAAAATTTGGGACTGTTCTTTGATTTTTAAACCCTTGCTCTCTCTAGTTATGTTAATAATTGCGTAGGTATCTCGACTGGGTCCAGAATTTGCATATGTGTTCATGCATCATATGGTATTCTCATAATTGTATCGACATAATTGCATCTGGAAAAAGAAAAGAAAAACGAACACCAGTTCTGGTATTATCTGCATAATTTTATTTTCTTAAATACTTTAGAACGTAAGGCTTTTGTCGAACATCCTTCTGTTGAATTTAAGGCTAAATGTTGGTTTTTCAGGATAACATATTTGAATGGCAATTCGCCATCCGCGGACCTGGTGATAGTGAATTTGAGGGTGGGATATACCATGGACGTATCCAATTGCCATCTGAATATCCATTCAAGCCACCTTCGTTCATGTTGTTAACCGTAAGATAGCAATCTCTTTTCCTCTACTTATTTCCCTTTTTGTTGATTCTCTTCTGTTGTGTTGATGGGCTGCTCAATGTCAATTCCTTTCTTTCAGCCAAATGGGCGTTTTGAAACCCAAACAAAGATTTGCTTAAGCATATCGAACCATCATCCCGAGCATTGGCAACCGTCATGGAGTGGTGAGTTCTTTGTATTCTTTATCCAGCTTGTCTTATTACATTGTTCCGAGTATGTAACAACCAAAATCCACGATATTGTCCTCTTTGAGCCTCTCATAGGGAGGTTTCTAGATCCTTGTGAATGTTTCGTTTCCCTCTCCAACCGACGTGGGATCTCACAATGTATATAATTCCTAACTTAAACATAACTCAACTGATTTAGGCTTAAGATTTTTGACTGAAAAGAACATACTTTGAATTCTTACTCACACATTAACAAGGATGGCATTGTCTGGTTTCTACTTAAGTTGCTTTAGGTTCCACTTCCACATTCTCTGATCCTTCTTGTTCTTTTCCTCCCTTCCCTTTTTGTTGATTGCTGCTTGTCAGTACGCACTGCCTTGGTTGCATTAATTGCTTTCATGCCCACAAACCCAAATGGCGCTCTTGGTTCCCTGGACTACAAGAAGGAAGAAAGACGGGTATTGGCCATCAAATCTCGAGAAGCAGCCCCCAAATTTGGTACTCCTGAACGTCAAAAGCTAATTGACGAGGTATTTCCAAGTTCTGTTGATATTTCATACCCTGTTGAATAGCATATTGAATTTCATTGTTCATTCATGAGCTTCTTTAACATCACAGATTCATGAATATTTGCTGAGTAAGGCGCCGCCTGCCCCTCAACCGAGTTCTCCAGATGACTCCGAGGAGAAACCTGCTAACAAAGAGCCTGATGTTGAGGTAACTTCTGAACCCGCTGAACTTGCAGCTGCTGTGGTAGAGCAACAGAATCCACCCATAAGCGACAGGATCGTCGAGGAAGCCATACCAGAGGAGACATCTATAAATACCAATCTCCAACAAGCAGCAGCAAGAGAGGCGCCATCTGATCGCCCCGCCGTGCAGTTGAGAACAGAGACGAGAGTCAATAAACCTGCCGATGACCGGTTGTTCACATGGGCTGCTGTGGGGCTCACTCTTGCAATTGTAGTTCTGTTGCTGAAGAAGTTCATCAAAGCAAGCACTCATGGATCAGTGTTCATGGATGAATCTTAATAAGATCACAGAAATGTCCGCCGCTGCTAGACACAAGGCTCCTTCTTATGTGTATCATGATTAGAGTCATTAGCGTAAATTTGAATTTCGTTATCATTGGACTAGGAAAAGGCAATCCTATGTCTTGTGATCCTGCCGCCCGCTTCCATTATTTGTAGCTATAATAGTAACATATGCAAGAGAGAAAAAAAAAATGCTTGGCTTAAAGCAGTGTGCCTAAAAACCCTTTTAAATATTTTGGCCGTCTATTTTATGTACATGGTGTTACCATATAATTACAATTGGTTTTGAGAACATGATGAAGTCTTCTTTTCTTCCTTTTGGAAGATTAATAGTAGTGTTGTGTTCTTTAGTTTTTCAATTTTTCTCCATTCATTTCCTAAAAATAATACACATAATTTTCTTTCTTTGTT

mRNA sequence

AAAATGACGTGGCGTGTTAATAGCCGACTTTTGTTCTTTCGCTCCCCTTCCTCCGTGCCCATAAATTGCTGTTCCCGTTCAATTTCTACGGATTGGCTTTGATTTCGATTCAAATCCTCTCGTCTGGTTTTAGGTTGAGTTTTTTTTTTTTCACCGTTCTCGATCGGATAATTTTTCGTCTCGTGATCGGGTGGCGGTTGCAGTCGGATCGGATCTGCGAGTTATTTGAAAAGATATGGCGGAGGATAAGTACAATTTGAAGAACCCTGCGGTGAAGAGGATCCTTCGGGAGGTCAAGGAGATGCAGGCTAATCCTTCTGATGATTTCATGAGTCTGCCTCTCGAGGATAACATATTTGAATGGCAATTCGCCATCCGCGGACCTGGTGATAGTGAATTTGAGGGTGGGATATACCATGGACGTATCCAATTGCCATCTGAATATCCATTCAAGCCACCTTCGTTCATGTTGTTAACCCCAAATGGGCGTTTTGAAACCCAAACAAAGATTTGCTTAAGCATATCGAACCATCATCCCGAGCATTGGCAACCGTCATGGAGTGTACGCACTGCCTTGGTTGCATTAATTGCTTTCATGCCCACAAACCCAAATGGCGCTCTTGGTTCCCTGGACTACAAGAAGGAAGAAAGACGGGTATTGGCCATCAAATCTCGAGAAGCAGCCCCCAAATTTGGTACTCCTGAACGTCAAAAGCTAATTGACGAGATTCATGAATATTTGCTGAGTAAGGCGCCGCCTGCCCCTCAACCGAGTTCTCCAGATGACTCCGAGGAGAAACCTGCTAACAAAGAGCCTGATGTTGAGGTAACTTCTGAACCCGCTGAACTTGCAGCTGCTGTGGTAGAGCAACAGAATCCACCCATAAGCGACAGGATCGTCGAGGAAGCCATACCAGAGGAGACATCTATAAATACCAATCTCCAACAAGCAGCAGCAAGAGAGGCGCCATCTGATCGCCCCGCCGTGCAGTTGAGAACAGAGACGAGAGTCAATAAACCTGCCGATGACCGGTTGTTCACATGGGCTGCTGTGGGGCTCACTCTTGCAATTGTAGTTCTGTTGCTGAAGAAGTTCATCAAAGCAAGCACTCATGGATCAGTGTTCATGGATGAATCTTAATAAGATCACAGAAATGTCCGCCGCTGCTAGACACAAGGCTCCTTCTTATGTGTATCATGATTAGAGTCATTAGCGTAAATTTGAATTTCGTTATCATTGGACTAGGAAAAGGCAATCCTATGTCTTGTGATCCTGCCGCCCGCTTCCATTATTTGTAGCTATAATAGTAACATATGCAAGAGAGAAAAAAAAAATGCTTGGCTTAAAGCAGTGTGCCTAAAAACCCTTTTAAATATTTTGGCCGTCTATTTTATGTACATGGTGTTACCATATAATTACAATTGGTTTTGAGAACATGATGAAGTCTTCTTTTCTTCCTTTTGGAAGATTAATAGTAGTGTTGTGTTCTTTAGTTTTTCAATTTTTCTCCATTCATTTCCTAAAAATAATACACATAATTTTCTTTCTTTGTT

Coding sequence (CDS)

ATGGCGGAGGATAAGTACAATTTGAAGAACCCTGCGGTGAAGAGGATCCTTCGGGAGGTCAAGGAGATGCAGGCTAATCCTTCTGATGATTTCATGAGTCTGCCTCTCGAGGATAACATATTTGAATGGCAATTCGCCATCCGCGGACCTGGTGATAGTGAATTTGAGGGTGGGATATACCATGGACGTATCCAATTGCCATCTGAATATCCATTCAAGCCACCTTCGTTCATGTTGTTAACCCCAAATGGGCGTTTTGAAACCCAAACAAAGATTTGCTTAAGCATATCGAACCATCATCCCGAGCATTGGCAACCGTCATGGAGTGTACGCACTGCCTTGGTTGCATTAATTGCTTTCATGCCCACAAACCCAAATGGCGCTCTTGGTTCCCTGGACTACAAGAAGGAAGAAAGACGGGTATTGGCCATCAAATCTCGAGAAGCAGCCCCCAAATTTGGTACTCCTGAACGTCAAAAGCTAATTGACGAGATTCATGAATATTTGCTGAGTAAGGCGCCGCCTGCCCCTCAACCGAGTTCTCCAGATGACTCCGAGGAGAAACCTGCTAACAAAGAGCCTGATGTTGAGGTAACTTCTGAACCCGCTGAACTTGCAGCTGCTGTGGTAGAGCAACAGAATCCACCCATAAGCGACAGGATCGTCGAGGAAGCCATACCAGAGGAGACATCTATAAATACCAATCTCCAACAAGCAGCAGCAAGAGAGGCGCCATCTGATCGCCCCGCCGTGCAGTTGAGAACAGAGACGAGAGTCAATAAACCTGCCGATGACCGGTTGTTCACATGGGCTGCTGTGGGGCTCACTCTTGCAATTGTAGTTCTGTTGCTGAAGAAGTTCATCAAAGCAAGCACTCATGGATCAGTGTTCATGGATGAATCTTAA

Protein sequence

MAEDKYNLKNPAVKRILREVKEMQANPSDDFMSLPLEDNIFEWQFAIRGPGDSEFEGGIYHGRIQLPSEYPFKPPSFMLLTPNGRFETQTKICLSISNHHPEHWQPSWSVRTALVALIAFMPTNPNGALGSLDYKKEERRVLAIKSREAAPKFGTPERQKLIDEIHEYLLSKAPPAPQPSSPDDSEEKPANKEPDVEVTSEPAELAAAVVEQQNPPISDRIVEEAIPEETSINTNLQQAAAREAPSDRPAVQLRTETRVNKPADDRLFTWAAVGLTLAIVVLLLKKFIKASTHGSVFMDES
Homology
[Close]
BLAST of Cp4.1LG08g05100 vs. Swiss-Prot
Match: UBC32_ARATH (Ubiquitin-conjugating enzyme E2 32 OS=Arabidopsis thaliana GN=UBC32 PE=2 SV=1)

HSP 1 Score: 389.0 bits (998), Expect = 4.6e-107
Identity = 205/311 (65.92%), Postives = 246/311 (79.10%), Query Frame = 1 

Query: 1   MAEDKYNLKNPAVKRILREVKEMQANPSDDFMSLPLEDNIFEWQFAIRGPGDSEFEGGIY 60
           MA+++YN KNPAVKRIL+EVKEMQANPSDDFMSLPLE+NIFEWQFAIRGPGD+EFEGGIY
Sbjct: 1   MADERYNRKNPAVKRILQEVKEMQANPSDDFMSLPLEENIFEWQFAIRGPGDTEFEGGIY 60

Query: 61  HGRIQLPSEYPFKPPSFMLLTPNGRFETQTKICLSISNHHPEHWQPSWSVRTALVALIAF 120
           HGRIQLP++YPFKPPSFMLLTPNGRFET TKICLSISN+HPEHWQPSWSVRTALVALIAF
Sbjct: 61  HGRIQLPADYPFKPPSFMLLTPNGRFETNTKICLSISNYHPEHWQPSWSVRTALVALIAF 120

Query: 121 MPTNPNGALGSLDYKKEERRVLAIKSREAAPKFGTPERQKLIDEIHEYLLSKAPPAPQPS 180
           MPT+PNGALGS+DY K+ERR LAIKSRE  PK+G+PERQK+IDEIH+Y+LSKA   P+P 
Sbjct: 121 MPTSPNGALGSVDYPKDERRTLAIKSRETPPKYGSPERQKIIDEIHQYILSKATVVPKPL 180

Query: 181 SPDDSEEKPANKEPDVEVTSEPAELAAAVVEQQNPPISDRIVEEAIPEETSINTNLQQAA 240
             + S+      E   +V  EP E A  VVE+++   +D IV++ I EET+   N   A+
Sbjct: 181 PLECSQAPSIVSEAHSQV--EPQE-AITVVEERSIATTDTIVDDQIIEETAEAVN-TAAS 240

Query: 241 AREAPSDRPAVQLRTETRVN-----------KPADDRLFTWAAVGLTLAIVVLLLKKFIK 300
              A +  PAV++  +  V+           KP DDRLFTWAAVGLT+AI+VLLLKKFIK
Sbjct: 241 VVPAAAPLPAVEVVVKASVSGEQRMARRAAQKPVDDRLFTWAAVGLTIAIMVLLLKKFIK 300

[Close]
BLAST of Cp4.1LG08g05100 vs. Swiss-Prot
Match: UB2J1_HUMAN (Ubiquitin-conjugating enzyme E2 J1 OS=Homo sapiens GN=UBE2J1 PE=1 SV=2)

HSP 1 Score: 213.4 bits (542), Expect = 3.4e-54
Identity = 95/146 (65.07%), Postives = 118/146 (80.82%), Query Frame = 1 

Query: 3   EDKYNLKNPAVKRILREVKEMQANPSDDFMSLPLEDNIFEWQFAIRGPGDSEFEGGIYHG 62
           E +YNLK+PAVKR+++E  E++ +P+D + + PLEDN+FEW F +RGP DS+F+GG+YHG
Sbjct: 2   ETRYNLKSPAVKRLMKEAAELK-DPTDHYHAQPLEDNLFEWHFTVRGPPDSDFDGGVYHG 61

Query: 63  RIQLPSEYPFKPPSFMLLTPNGRFETQTKICLSISNHHPEHWQPSWSVRTALVALIAFMP 122
           RI LP EYP KPPS +LLT NGRFE   KICLSIS HHPE WQPSWS+RTAL+A+I FMP
Sbjct: 62  RIVLPPEYPMKPPSIILLTANGRFEVGKKICLSISGHHPETWQPSWSIRTALLAIIGFMP 121

Query: 123 TNPNGALGSLDYKKEERRVLAIKSRE 149
           T   GA+GSLDY  EERR LA KS++
Sbjct: 122 TKGEGAIGSLDYTPEERRALAKKSQD 146

[Close]
BLAST of Cp4.1LG08g05100 vs. Swiss-Prot
Match: UB2J1_MOUSE (Ubiquitin-conjugating enzyme E2 J1 OS=Mus musculus GN=Ube2j1 PE=1 SV=2)

HSP 1 Score: 213.4 bits (542), Expect = 3.4e-54
Identity = 95/146 (65.07%), Postives = 118/146 (80.82%), Query Frame = 1 

Query: 3   EDKYNLKNPAVKRILREVKEMQANPSDDFMSLPLEDNIFEWQFAIRGPGDSEFEGGIYHG 62
           E +YNLK+PAVKR+++E  E++ +P+D + + PLEDN+FEW F +RGP DS+F+GG+YHG
Sbjct: 2   ETRYNLKSPAVKRLMKEAAELK-DPTDHYHAQPLEDNLFEWHFTVRGPPDSDFDGGVYHG 61

Query: 63  RIQLPSEYPFKPPSFMLLTPNGRFETQTKICLSISNHHPEHWQPSWSVRTALVALIAFMP 122
           RI LP EYP KPPS +LLT NGRFE   KICLSIS HHPE WQPSWS+RTAL+A+I FMP
Sbjct: 62  RIVLPPEYPMKPPSIILLTANGRFEVGKKICLSISGHHPETWQPSWSIRTALLAIIGFMP 121

Query: 123 TNPNGALGSLDYKKEERRVLAIKSRE 149
           T   GA+GSLDY  EERR LA KS++
Sbjct: 122 TKGEGAIGSLDYTPEERRALAKKSQD 146

[Close]
BLAST of Cp4.1LG08g05100 vs. Swiss-Prot
Match: UBC6_YEAST (Ubiquitin-conjugating enzyme E2 6 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) GN=UBC6 PE=1 SV=1)

HSP 1 Score: 140.6 bits (353), Expect = 2.8e-32
Identity = 60/135 (44.44%), Postives = 88/135 (65.19%), Query Frame = 1 

Query: 12  AVKRILREVKEMQANPSDDFMSLPLEDNIFEWQFAIRGPGDSEFEGGIYHGRIQLPSEYP 71
           A KR+ +E K M  NP    ++ P EDNI EW + I GP D+ ++GG YHG +  PS+YP
Sbjct: 6   AHKRLTKEYKLMVENPPPYILARPNEDNILEWHYIITGPADTPYKGGQYHGTLTFPSDYP 65

Query: 72  FKPPSFMLLTPNGRFETQTKICLSISNHHPEHWQPSWSVRTALVALIAFMPTNPNGALGS 131
           +KPP+  ++TPNGRF+  T++CLS+S++HP+ W P WSV T L  L++FM T+     GS
Sbjct: 66  YKPPAIRMITPNGRFKPNTRLCLSMSDYHPDTWNPGWSVSTILNGLLSFM-TSDEATTGS 125

Query: 132 LDYKKEERRVLAIKS 147
           +     +++ LA  S
Sbjct: 126 ITTSDHQKKTLARNS 139

[Close]
BLAST of Cp4.1LG08g05100 vs. Swiss-Prot
Match: UBC6_ASHGO (Ubiquitin-conjugating enzyme E2 6 OS=Ashbya gossypii (strain ATCC 10895 / CBS 109.51 / FGSC 9923 / NRRL Y-1056) GN=UBC6 PE=3 SV=1)

HSP 1 Score: 140.6 bits (353), Expect = 2.8e-32
Identity = 60/137 (43.80%), Postives = 89/137 (64.96%), Query Frame = 1 

Query: 12  AVKRILREVKEMQANPSDDFMSLPLEDNIFEWQFAIRGPGDSEFEGGIYHGRIQLPSEYP 71
           A KR+ +E K M  NP    ++ P EDNI  W + I GP ++ +E G YHG +  P++YP
Sbjct: 6   AYKRLSKEYKMMTENPPPYIVAAPKEDNILVWHYVITGPPETPYEDGQYHGTLVFPNDYP 65

Query: 72  FKPPSFMLLTPNGRFETQTKICLSISNHHPEHWQPSWSVRTALVALIAFMPTNPNGALGS 131
           F PP+  +LTPNGRF   T++CLS+S++HP+ W PSWSV T L  L++FM T+   ++G+
Sbjct: 66  FNPPAIRMLTPNGRFRENTRLCLSMSDYHPDTWNPSWSVATILTGLLSFMTTD-ESSIGT 125

Query: 132 LDYKKEERRVLAIKSRE 149
           ++     +R  A KS+E
Sbjct: 126 MESTDSTKRKYAAKSKE 141

[Close]
BLAST of Cp4.1LG08g05100 vs. TrEMBL
Match: A0A0A0L954_CUCSA (Uncharacterized protein OS=Cucumis sativus GN=Csa_3G166270 PE=4 SV=1)

HSP 1 Score: 499.2 bits (1284), Expect = 3.5e-138
Identity = 257/304 (84.54%), Postives = 269/304 (88.49%), Query Frame = 1 

Query: 1   MAEDKYNLKNPAVKRILREVKEMQANPSDDFMSLPLEDNIFEWQFAIRGPGDSEFEGGIY 60
           MAEDKYNLKNPAVKRIL+EVKEMQANPSDDFMSLPLE+NIFEWQFAIRGPGDSEFEGGIY
Sbjct: 1   MAEDKYNLKNPAVKRILQEVKEMQANPSDDFMSLPLEENIFEWQFAIRGPGDSEFEGGIY 60

Query: 61  HGRIQLPSEYPFKPPSFMLLTPNGRFETQTKICLSISNHHPEHWQPSWSVRTALVALIAF 120
           HGRIQLP+EYPFKPPSFMLLTPNGRFETQTKICLSISNHHPEHWQPSWSVRTALVALIAF
Sbjct: 61  HGRIQLPAEYPFKPPSFMLLTPNGRFETQTKICLSISNHHPEHWQPSWSVRTALVALIAF 120

Query: 121 MPTNPNGALGSLDYKKEERRVLAIKSREAAPKFGTPERQKLIDEIHEYLLSKAPPAPQPS 180
           MPTNPNGALGSLDYKKEERRVLAIKSREA PKFGTPERQKLIDEIHEY+LSKAPP PQ  
Sbjct: 121 MPTNPNGALGSLDYKKEERRVLAIKSREAPPKFGTPERQKLIDEIHEYMLSKAPPVPQSG 180

Query: 181 SPDDSEEKPANKEPDVEVTSEPAELAAAVVEQQNPPISDRIVEEAIPEETSINTNLQQAA 240
           S D S EKP N+E DV+VTSE AELAAAV E QN P  +RIVEEA+PE       +Q   
Sbjct: 181 STDVSGEKPTNEEADVQVTSETAELAAAVEELQNSPTINRIVEEAVPE-------VQPEV 240

Query: 241 AREAPSDRPAVQL---RTETRVNKPADDRLFTWAAVGLTLAIVVLLLKKFIKASTHGSVF 300
           AR APS RP  Q    R ETRV+KPADDRLFTWAA+GLTLAIVVLLLKKF+KAS HGSVF
Sbjct: 241 ARVAPSSRPTTQTEQGRPETRVHKPADDRLFTWAAIGLTLAIVVLLLKKFMKASAHGSVF 297

Query: 301 MDES 302
           MDES
Sbjct: 301 MDES 297

[Close]
BLAST of Cp4.1LG08g05100 vs. TrEMBL
Match: V4RY15_9ROSI (Uncharacterized protein OS=Citrus clementina GN=CICLE_v10005518mg PE=4 SV=1)

HSP 1 Score: 447.6 bits (1150), Expect = 1.2e-122
Identity = 232/305 (76.07%), Postives = 261/305 (85.57%), Query Frame = 1 

Query: 1   MAEDKYNLKNPAVKRILREVKEMQANPSDDFMSLPLEDNIFEWQFAIRGPGDSEFEGGIY 60
           MAED+YNLKNPAVKRIL+EVKEMQ+NPSDDFMSLPLE+NIFEWQFAIRGPGD+EFEGGIY
Sbjct: 1   MAEDRYNLKNPAVKRILQEVKEMQSNPSDDFMSLPLEENIFEWQFAIRGPGDTEFEGGIY 60

Query: 61  HGRIQLPSEYPFKPPSFMLLTPNGRFETQTKICLSISNHHPEHWQPSWSVRTALVALIAF 120
           HGRIQLP+EYPFKPPSFMLLTPNGRFETQTKICLSISNHHPEHWQPSWSVRTALVALIAF
Sbjct: 61  HGRIQLPAEYPFKPPSFMLLTPNGRFETQTKICLSISNHHPEHWQPSWSVRTALVALIAF 120

Query: 121 MPTNPNGALGSLDYKKEERRVLAIKSREAAPKFGTPERQKLIDEIHEYLLSKAPPAPQPS 180
           MPTNPNGALGSLDYKKEERR LAIKSREAAPKFGTPERQKLIDEIHEY+LSKAPP PQ S
Sbjct: 121 MPTNPNGALGSLDYKKEERRALAIKSREAAPKFGTPERQKLIDEIHEYMLSKAPPVPQLS 180

Query: 181 SPDDSEEKPANKEPDVEVTSEPAELAAAVVEQQNPPISDRIVEEAIPE-ETSINTN-LQQ 240
           +    EE+P N+E + + +S  A +A A  E    P+ DRI+EE   +   S+N N +  
Sbjct: 181 T---CEEQPGNREGEAQASSIDAMVAGA-GEGLPAPVGDRIIEEVQEDLPASMNPNPVVA 240

Query: 241 AAAREAPSDRPAVQL--RTETRVNKPADDRLFTWAAVGLTLAIVVLLLKKFIKASTHGSV 300
             +RE P++   VQL  + ETRV KPADDRLFTWAAVGLT+AI+VLLLKKF+K++ HG+V
Sbjct: 241 GTSREVPANASGVQLQPKPETRVQKPADDRLFTWAAVGLTIAILVLLLKKFMKSNGHGAV 300

Query: 301 FMDES 302
           FMD S
Sbjct: 301 FMDGS 301

[Close]
BLAST of Cp4.1LG08g05100 vs. TrEMBL
Match: A0A067FWY2_CITSI (Uncharacterized protein OS=Citrus sinensis GN=CISIN_1g020053mg PE=4 SV=1)

HSP 1 Score: 446.0 bits (1146), Expect = 3.5e-122
Identity = 231/305 (75.74%), Postives = 260/305 (85.25%), Query Frame = 1 

Query: 1   MAEDKYNLKNPAVKRILREVKEMQANPSDDFMSLPLEDNIFEWQFAIRGPGDSEFEGGIY 60
           MAED+YNLKNPAVKRIL+EVKEMQ+NPSDDFMSLPLE+NIFEWQFAIRGPGD+EFEGGIY
Sbjct: 1   MAEDRYNLKNPAVKRILQEVKEMQSNPSDDFMSLPLEENIFEWQFAIRGPGDTEFEGGIY 60

Query: 61  HGRIQLPSEYPFKPPSFMLLTPNGRFETQTKICLSISNHHPEHWQPSWSVRTALVALIAF 120
           HGRIQLP+EYPFKPPSFMLLTPNGRFETQTKICLSISNHHPEHWQPSWSVRTALVALIAF
Sbjct: 61  HGRIQLPAEYPFKPPSFMLLTPNGRFETQTKICLSISNHHPEHWQPSWSVRTALVALIAF 120

Query: 121 MPTNPNGALGSLDYKKEERRVLAIKSREAAPKFGTPERQKLIDEIHEYLLSKAPPAPQPS 180
           MPTNPNGALGSLDYKKEERR LAIKSREAAPKFGTPERQKLIDEIHEY+LSKAPP PQ S
Sbjct: 121 MPTNPNGALGSLDYKKEERRALAIKSREAAPKFGTPERQKLIDEIHEYMLSKAPPVPQLS 180

Query: 181 SPDDSEEKPANKEPDVEVTSEPAELAAAVVEQQNPPISDRIVEEAIPE-ETSINTN-LQQ 240
           +    EE+P N+E + + +S  A +  A  E    P+ DRI+EE   +   S+N N +  
Sbjct: 181 T---CEEQPGNREGEAQASSIDAMVTGA-GEGLPAPVGDRIIEEVQEDLPASMNPNPVVA 240

Query: 241 AAAREAPSDRPAVQL--RTETRVNKPADDRLFTWAAVGLTLAIVVLLLKKFIKASTHGSV 300
             +RE P++   VQL  + ETRV KPADDRLFTWAAVGLT+AI+VLLLKKF+K++ HG+V
Sbjct: 241 GTSREVPANASGVQLQPKPETRVQKPADDRLFTWAAVGLTIAILVLLLKKFMKSNGHGTV 300

Query: 301 FMDES 302
           FMD S
Sbjct: 301 FMDGS 301

[Close]
BLAST of Cp4.1LG08g05100 vs. TrEMBL
Match: B9RZP3_RICCO (Non-canonical ubiquitin conjugating enzyme, putative OS=Ricinus communis GN=RCOM_1000310 PE=4 SV=1)

HSP 1 Score: 434.9 bits (1117), Expect = 8.1e-119
Identity = 232/310 (74.84%), Postives = 253/310 (81.61%), Query Frame = 1 

Query: 1   MAEDKYNLKNPAVKRILREVKEMQANPSDDFMSLPLEDNIFEWQFAIRGPGDSEFEGGIY 60
           MAEDKYNLKNPAVKRIL+EVKEMQ+NPSDDFMS+PLE+NIFEWQFAIRGP D+EFEGGIY
Sbjct: 1   MAEDKYNLKNPAVKRILQEVKEMQSNPSDDFMSMPLEENIFEWQFAIRGPCDTEFEGGIY 60

Query: 61  HGRIQLPSEYPFKPPSFMLLTPNGRFETQTKICLSISNHHPEHWQPSWSVRTALVALIAF 120
           HGRIQLPSEYPFKPPSF+LLTPNGRFETQTKICLSISNHHPEHWQPSWSVRTALVALIAF
Sbjct: 61  HGRIQLPSEYPFKPPSFLLLTPNGRFETQTKICLSISNHHPEHWQPSWSVRTALVALIAF 120

Query: 121 MPTNPNGALGSLDYKKEERRVLAIKSREAAPKFGTPERQKLIDEIHEYLLSKAPPAPQPS 180
           MPTNPNGALGSLDYKKEERR LAIKSREAAP+FGTPERQKLIDEIH+Y+LSKAPP PQ S
Sbjct: 121 MPTNPNGALGSLDYKKEERRALAIKSREAAPRFGTPERQKLIDEIHQYMLSKAPPVPQLS 180

Query: 181 SPDDSEEKPANKEPDVE-VTSEPAELAA-----AVVEQQNPPISDRIVEEAIPEETSINT 240
              DSEE    KE +   +  E   +AA     AV EQ    + DRIVEE    E  +N 
Sbjct: 181 PSQDSEEHHTGKEDEAHAILQEQGAVAANLPNLAVSEQL--AVGDRIVEEV--PEAPVNV 240

Query: 241 NLQQAAAR---EAPSDRPAVQL--RTETRVNKPADDRLFTWAAVGLTLAIVVLLLKKFIK 300
           N      R   E P+   + QL  R ETRV +PADDRLFTWAAVGLT+AIVVLLLKKF+K
Sbjct: 241 NPGATGVRTSTEIPARASSDQLLHRPETRVQRPADDRLFTWAAVGLTIAIVVLLLKKFLK 300

[Close]
BLAST of Cp4.1LG08g05100 vs. TrEMBL
Match: W9RG08_9ROSA (Ubiquitin-conjugating enzyme E2 32 OS=Morus notabilis GN=L484_004205 PE=4 SV=1)

HSP 1 Score: 430.6 bits (1106), Expect = 1.5e-117
Identity = 217/302 (71.85%), Postives = 245/302 (81.13%), Query Frame = 1 

Query: 1   MAEDKYNLKNPAVKRILREVKEMQANPSDDFMSLPLEDNIFEWQFAIRGPGDSEFEGGIY 60
           MAEDKYNLKNPAVKRIL+E+KEMQ+N SDDFMSLPLE+NIFEWQFAIRGP D+EFEGGIY
Sbjct: 1   MAEDKYNLKNPAVKRILQEIKEMQSNSSDDFMSLPLEENIFEWQFAIRGPRDTEFEGGIY 60

Query: 61  HGRIQLPSEYPFKPPSFMLLTPNGRFETQTKICLSISNHHPEHWQPSWSVRTALVALIAF 120
           HGRIQLP+EYPFKPPSFMLLTPNGRFETQTKICLSISNHHPEHWQPSWSVRTALVALIAF
Sbjct: 61  HGRIQLPAEYPFKPPSFMLLTPNGRFETQTKICLSISNHHPEHWQPSWSVRTALVALIAF 120

Query: 121 MPTNPNGALGSLDYKKEERRVLAIKSREAAPKFGTPERQKLIDEIHEYLLSKAPPAPQPS 180
           MPT+PNGALGSLDYKKEERR LA+KSREA+PKFGTPERQKLIDE+HEY+LSKAPP PQ  
Sbjct: 121 MPTSPNGALGSLDYKKEERRALAVKSREASPKFGTPERQKLIDEVHEYMLSKAPPVPQLC 180

Query: 181 SPDDSEEKPAN-KEPDVEVTSEPAELAAAVVEQQNPPISDRIVEEAIPEETSINTNLQQA 240
             +  EE+P   +E D +  S+ +++A A     NP + +R+VEE      + N  L + 
Sbjct: 181 PSETPEEQPTKIEEGDAQAGSQNSDVAQAAEGLPNPSVGERVVEEHQESPANANPRLARE 240

Query: 241 AAREAPSDRPAVQLRTETRVNKPADDRLFTWAAVGLTLAIVVLLLKKFIKASTHGSVFMD 300
            A         V  R E R  +PADDRLFTWAAVGLT+AIVVLLLKKF+ A  HG+VFMD
Sbjct: 241 RAPSEVHSSNQVLQRQEVRAQRPADDRLFTWAAVGLTIAIVVLLLKKFMNAGGHGAVFMD 300

Query: 301 ES 302
            S
Sbjct: 301 GS 302

[Close]
BLAST of Cp4.1LG08g05100 vs. TAIR10
Match: AT3G17000.1 (AT3G17000.1 ubiquitin-conjugating enzyme 32)

HSP 1 Score: 389.0 bits (998), Expect = 2.6e-108
Identity = 205/311 (65.92%), Postives = 246/311 (79.10%), Query Frame = 1 

Query: 1   MAEDKYNLKNPAVKRILREVKEMQANPSDDFMSLPLEDNIFEWQFAIRGPGDSEFEGGIY 60
           MA+++YN KNPAVKRIL+EVKEMQANPSDDFMSLPLE+NIFEWQFAIRGPGD+EFEGGIY
Sbjct: 1   MADERYNRKNPAVKRILQEVKEMQANPSDDFMSLPLEENIFEWQFAIRGPGDTEFEGGIY 60

Query: 61  HGRIQLPSEYPFKPPSFMLLTPNGRFETQTKICLSISNHHPEHWQPSWSVRTALVALIAF 120
           HGRIQLP++YPFKPPSFMLLTPNGRFET TKICLSISN+HPEHWQPSWSVRTALVALIAF
Sbjct: 61  HGRIQLPADYPFKPPSFMLLTPNGRFETNTKICLSISNYHPEHWQPSWSVRTALVALIAF 120

Query: 121 MPTNPNGALGSLDYKKEERRVLAIKSREAAPKFGTPERQKLIDEIHEYLLSKAPPAPQPS 180
           MPT+PNGALGS+DY K+ERR LAIKSRE  PK+G+PERQK+IDEIH+Y+LSKA   P+P 
Sbjct: 121 MPTSPNGALGSVDYPKDERRTLAIKSRETPPKYGSPERQKIIDEIHQYILSKATVVPKPL 180

Query: 181 SPDDSEEKPANKEPDVEVTSEPAELAAAVVEQQNPPISDRIVEEAIPEETSINTNLQQAA 240
             + S+      E   +V  EP E A  VVE+++   +D IV++ I EET+   N   A+
Sbjct: 181 PLECSQAPSIVSEAHSQV--EPQE-AITVVEERSIATTDTIVDDQIIEETAEAVN-TAAS 240

Query: 241 AREAPSDRPAVQLRTETRVN-----------KPADDRLFTWAAVGLTLAIVVLLLKKFIK 300
              A +  PAV++  +  V+           KP DDRLFTWAAVGLT+AI+VLLLKKFIK
Sbjct: 241 VVPAAAPLPAVEVVVKASVSGEQRMARRAAQKPVDDRLFTWAAVGLTIAIMVLLLKKFIK 300

[Close]
BLAST of Cp4.1LG08g05100 vs. TAIR10
Match: AT5G50430.1 (AT5G50430.1 ubiquitin-conjugating enzyme 33)

HSP 1 Score: 119.8 bits (299), Expect = 2.9e-27
Identity = 62/187 (33.16%), Postives = 104/187 (55.61%), Query Frame = 1 

Query: 13  VKRILREVKEMQANPSDDFMSLPLEDNIFEWQFAIRGPGDSEFEGGIYHGRIQLPSEYPF 72
           +KR+ +E + +   P    ++ P  ++I EW + + G   + F GG Y+G+I+ P EYP+
Sbjct: 7   IKRLQKEYRALCKEPVSHVVARPSPNDILEWHYVLEGSEGTPFAGGFYYGKIKFPPEYPY 66

Query: 73  KPPSFMLLTPNGRFETQTKICLSISNHHPEHWQPSWSVRTALVALIAFMPTNPNGALGSL 132
           KPP   + TPNGRF TQ KICLS+S+ HPE W P WSV + L  L++FM  N +   GS+
Sbjct: 67  KPPGITMTTPNGRFVTQKKICLSMSDFHPESWNPMWSVSSILTGLLSFMMDN-SPTTGSV 126

Query: 133 DYKKEERRVLAIKSREAAPKFGTPER--QKLIDEIHEYLLSKAPPAPQPSSPDDSEEKPA 192
           +    E++ LA  S     K  T  +   + +++  +  +++   A Q ++  ++++ P 
Sbjct: 127 NTSVAEKQRLAKSSLAFNCKSVTFRKLFPEYVEKYSQQQVAEEEAATQQTTTSENQDFPQ 186

Query: 193 NKEPDVE 198
                VE
Sbjct: 187 KDNAKVE 192

[Close]
BLAST of Cp4.1LG08g05100 vs. TAIR10
Match: AT1G17280.1 (AT1G17280.1 ubiquitin-conjugating enzyme 34)

HSP 1 Score: 119.0 bits (297), Expect = 5.0e-27
Identity = 69/186 (37.10%), Postives = 103/186 (55.38%), Query Frame = 1 

Query: 13  VKRILREVKEMQANPSDDFMSLPLEDNIFEWQFAIRGPGDSEFEGGIYHGRIQLPSEYPF 72
           +KR+ +E + +   P    ++ P  ++I EW + + G   + F GG Y+G+I+ P EYP+
Sbjct: 7   IKRLQKEYRALCKEPVSHVVARPSPNDILEWHYVLEGSEGTPFAGGFYYGKIKFPPEYPY 66

Query: 73  KPPSFMLLTPNGRFETQTKICLSISNHHPEHWQPSWSVRTALVALIAF-MPTNPNGALGS 132
           KPP   + TPNGRF TQ KICLS+S+ HPE W P WSV + L  L++F M T+P    GS
Sbjct: 67  KPPGITMTTPNGRFMTQKKICLSMSDFHPESWNPMWSVSSILTGLLSFMMDTSP--TTGS 126

Query: 133 LDYKKEERRVLAIKSREAAPKFGTPERQKLIDEIHEYLLSKAPPAPQPSSPDDSEEKPAN 192
           ++    E++ LA  S   A    TP  +KL  E  E   ++   A Q ++   + E P  
Sbjct: 127 VNTTVIEKQRLAKSS--LAFNCKTPAFRKLFPEYVE-KYNQQQLAEQATTQLTTPESPQK 186

Query: 193 KEPDVE 198
            +  VE
Sbjct: 187 SDTKVE 187

[Close]
BLAST of Cp4.1LG08g05100 vs. TAIR10
Match: AT4G27960.2 (AT4G27960.2 ubiquitin conjugating enzyme 9)

HSP 1 Score: 82.8 bits (203), Expect = 4.0e-16
Identity = 40/117 (34.19%), Postives = 65/117 (55.56%), Query Frame = 1 

Query: 12  AVKRILREVKEMQANPSDDFMSLPLEDNIFEWQFAIRGPGDSEFEGGIYHGRIQLPSEYP 71
           A KRIL+E+K++Q +P     + P+ +++F WQ  I GP DS + GG++   I  P +YP
Sbjct: 32  ASKRILKELKDLQKDPPTSCSAGPVAEDMFHWQATIMGPSDSPYSGGVFLVTIHFPPDYP 91

Query: 72  FKPPSFMLLTP--NGRFETQTKICLSISNHHPEHWQPSWSVRTALVALIAFMPTNPN 127
           FKPP     T   +    +   ICL I     E W P+ ++   L+++ + + T+PN
Sbjct: 92  FKPPKVAFRTKVFHPNINSNGSICLDILK---EQWSPALTISKVLLSICSLL-TDPN 144

[Close]
BLAST of Cp4.1LG08g05100 vs. TAIR10
Match: AT3G08690.1 (AT3G08690.1 ubiquitin-conjugating enzyme 11)

HSP 1 Score: 82.0 bits (201), Expect = 6.8e-16
Identity = 40/117 (34.19%), Postives = 67/117 (57.26%), Query Frame = 1 

Query: 12  AVKRILREVKEMQANPSDDFMSLPLEDNIFEWQFAIRGPGDSEFEGGIYHGRIQLPSEYP 71
           A KRIL+E+K++Q +P  +  + P+ +++F WQ  I GP +S + GG++   I  P +YP
Sbjct: 2   ASKRILKELKDLQKDPPSNCSAGPVAEDMFHWQATIMGPPESPYAGGVFLVSIHFPPDYP 61

Query: 72  FKPP--SFMLLTPNGRFETQTKICLSISNHHPEHWQPSWSVRTALVALIAFMPTNPN 127
           FKPP  SF     +    +   ICL I     E W P+ ++   L+++ + + T+PN
Sbjct: 62  FKPPKVSFKTKVYHPNINSNGSICLDILK---EQWSPALTISKVLLSICSLL-TDPN 114

[Close]
BLAST of Cp4.1LG08g05100 vs. NCBI nr
Match: gi|659076938|ref|XP_008438945.1| (PREDICTED: ubiquitin-conjugating enzyme E2 32 [Cucumis melo])

HSP 1 Score: 500.0 bits (1286), Expect = 2.9e-138
Identity = 256/304 (84.21%), Postives = 271/304 (89.14%), Query Frame = 1 

Query: 1   MAEDKYNLKNPAVKRILREVKEMQANPSDDFMSLPLEDNIFEWQFAIRGPGDSEFEGGIY 60
           MAEDKYN+KNPAVKRIL+EVKEMQANPSDDFMSLPLE+NIFEWQFAIRGP DSEFEGGIY
Sbjct: 1   MAEDKYNMKNPAVKRILQEVKEMQANPSDDFMSLPLEENIFEWQFAIRGPDDSEFEGGIY 60

Query: 61  HGRIQLPSEYPFKPPSFMLLTPNGRFETQTKICLSISNHHPEHWQPSWSVRTALVALIAF 120
           HGRIQLP+EYPFKPPSFMLLTPNGRFETQTKICLSISNHHPEHWQPSWSVRTALVALIAF
Sbjct: 61  HGRIQLPAEYPFKPPSFMLLTPNGRFETQTKICLSISNHHPEHWQPSWSVRTALVALIAF 120

Query: 121 MPTNPNGALGSLDYKKEERRVLAIKSREAAPKFGTPERQKLIDEIHEYLLSKAPPAPQPS 180
           MPTNPNGALGSLDYKKEERRVLAIKSREA PKFGTPERQKLIDEIHEY+LSKAPP PQ  
Sbjct: 121 MPTNPNGALGSLDYKKEERRVLAIKSREAPPKFGTPERQKLIDEIHEYMLSKAPPVPQSG 180

Query: 181 SPDDSEEKPANKEPDVEVTSEPAELAAAVVEQQNPPISDRIVEEAIPEETSINTNLQQAA 240
           S D S++KP N+E DV+VTSEPAELAAA  E QNPP  +RIVEEA+PE       +Q   
Sbjct: 181 SSDVSDQKPTNEESDVQVTSEPAELAAAGEELQNPPTINRIVEEAVPE-------VQPEV 240

Query: 241 AREAPSDRP---AVQLRTETRVNKPADDRLFTWAAVGLTLAIVVLLLKKFIKASTHGSVF 300
           AR APS RP   A Q R ETRV+KPADDRLFTWAA+GLTLAIVVLLLKKF+KAS HGSVF
Sbjct: 241 ARVAPSSRPTTQAEQQRPETRVHKPADDRLFTWAAIGLTLAIVVLLLKKFMKASAHGSVF 297

Query: 301 MDES 302
           MDES
Sbjct: 301 MDES 297

[Close]
BLAST of Cp4.1LG08g05100 vs. NCBI nr
Match: gi|449432914|ref|XP_004134243.1| (PREDICTED: ubiquitin-conjugating enzyme E2 32 [Cucumis sativus])

HSP 1 Score: 499.2 bits (1284), Expect = 5.0e-138
Identity = 257/304 (84.54%), Postives = 269/304 (88.49%), Query Frame = 1 

Query: 1   MAEDKYNLKNPAVKRILREVKEMQANPSDDFMSLPLEDNIFEWQFAIRGPGDSEFEGGIY 60
           MAEDKYNLKNPAVKRIL+EVKEMQANPSDDFMSLPLE+NIFEWQFAIRGPGDSEFEGGIY
Sbjct: 1   MAEDKYNLKNPAVKRILQEVKEMQANPSDDFMSLPLEENIFEWQFAIRGPGDSEFEGGIY 60

Query: 61  HGRIQLPSEYPFKPPSFMLLTPNGRFETQTKICLSISNHHPEHWQPSWSVRTALVALIAF 120
           HGRIQLP+EYPFKPPSFMLLTPNGRFETQTKICLSISNHHPEHWQPSWSVRTALVALIAF
Sbjct: 61  HGRIQLPAEYPFKPPSFMLLTPNGRFETQTKICLSISNHHPEHWQPSWSVRTALVALIAF 120

Query: 121 MPTNPNGALGSLDYKKEERRVLAIKSREAAPKFGTPERQKLIDEIHEYLLSKAPPAPQPS 180
           MPTNPNGALGSLDYKKEERRVLAIKSREA PKFGTPERQKLIDEIHEY+LSKAPP PQ  
Sbjct: 121 MPTNPNGALGSLDYKKEERRVLAIKSREAPPKFGTPERQKLIDEIHEYMLSKAPPVPQSG 180

Query: 181 SPDDSEEKPANKEPDVEVTSEPAELAAAVVEQQNPPISDRIVEEAIPEETSINTNLQQAA 240
           S D S EKP N+E DV+VTSE AELAAAV E QN P  +RIVEEA+PE       +Q   
Sbjct: 181 STDVSGEKPTNEEADVQVTSETAELAAAVEELQNSPTINRIVEEAVPE-------VQPEV 240

Query: 241 AREAPSDRPAVQL---RTETRVNKPADDRLFTWAAVGLTLAIVVLLLKKFIKASTHGSVF 300
           AR APS RP  Q    R ETRV+KPADDRLFTWAA+GLTLAIVVLLLKKF+KAS HGSVF
Sbjct: 241 ARVAPSSRPTTQTEQGRPETRVHKPADDRLFTWAAIGLTLAIVVLLLKKFMKASAHGSVF 297

Query: 301 MDES 302
           MDES
Sbjct: 301 MDES 297

[Close]
BLAST of Cp4.1LG08g05100 vs. NCBI nr
Match: gi|567852467|ref|XP_006419397.1| (hypothetical protein CICLE_v10005518mg [Citrus clementina])

HSP 1 Score: 447.6 bits (1150), Expect = 1.7e-122
Identity = 232/305 (76.07%), Postives = 261/305 (85.57%), Query Frame = 1 

Query: 1   MAEDKYNLKNPAVKRILREVKEMQANPSDDFMSLPLEDNIFEWQFAIRGPGDSEFEGGIY 60
           MAED+YNLKNPAVKRIL+EVKEMQ+NPSDDFMSLPLE+NIFEWQFAIRGPGD+EFEGGIY
Sbjct: 1   MAEDRYNLKNPAVKRILQEVKEMQSNPSDDFMSLPLEENIFEWQFAIRGPGDTEFEGGIY 60

Query: 61  HGRIQLPSEYPFKPPSFMLLTPNGRFETQTKICLSISNHHPEHWQPSWSVRTALVALIAF 120
           HGRIQLP+EYPFKPPSFMLLTPNGRFETQTKICLSISNHHPEHWQPSWSVRTALVALIAF
Sbjct: 61  HGRIQLPAEYPFKPPSFMLLTPNGRFETQTKICLSISNHHPEHWQPSWSVRTALVALIAF 120

Query: 121 MPTNPNGALGSLDYKKEERRVLAIKSREAAPKFGTPERQKLIDEIHEYLLSKAPPAPQPS 180
           MPTNPNGALGSLDYKKEERR LAIKSREAAPKFGTPERQKLIDEIHEY+LSKAPP PQ S
Sbjct: 121 MPTNPNGALGSLDYKKEERRALAIKSREAAPKFGTPERQKLIDEIHEYMLSKAPPVPQLS 180

Query: 181 SPDDSEEKPANKEPDVEVTSEPAELAAAVVEQQNPPISDRIVEEAIPE-ETSINTN-LQQ 240
           +    EE+P N+E + + +S  A +A A  E    P+ DRI+EE   +   S+N N +  
Sbjct: 181 T---CEEQPGNREGEAQASSIDAMVAGA-GEGLPAPVGDRIIEEVQEDLPASMNPNPVVA 240

Query: 241 AAAREAPSDRPAVQL--RTETRVNKPADDRLFTWAAVGLTLAIVVLLLKKFIKASTHGSV 300
             +RE P++   VQL  + ETRV KPADDRLFTWAAVGLT+AI+VLLLKKF+K++ HG+V
Sbjct: 241 GTSREVPANASGVQLQPKPETRVQKPADDRLFTWAAVGLTIAILVLLLKKFMKSNGHGAV 300

Query: 301 FMDES 302
           FMD S
Sbjct: 301 FMDGS 301

[Close]
BLAST of Cp4.1LG08g05100 vs. NCBI nr
Match: gi|568871343|ref|XP_006488848.1| (PREDICTED: ubiquitin-conjugating enzyme E2 32 [Citrus sinensis])

HSP 1 Score: 446.0 bits (1146), Expect = 5.0e-122
Identity = 231/305 (75.74%), Postives = 260/305 (85.25%), Query Frame = 1 

Query: 1   MAEDKYNLKNPAVKRILREVKEMQANPSDDFMSLPLEDNIFEWQFAIRGPGDSEFEGGIY 60
           MAED+YNLKNPAVKRIL+EVKEMQ+NPSDDFMSLPLE+NIFEWQFAIRGPGD+EFEGGIY
Sbjct: 1   MAEDRYNLKNPAVKRILQEVKEMQSNPSDDFMSLPLEENIFEWQFAIRGPGDTEFEGGIY 60

Query: 61  HGRIQLPSEYPFKPPSFMLLTPNGRFETQTKICLSISNHHPEHWQPSWSVRTALVALIAF 120
           HGRIQLP+EYPFKPPSFMLLTPNGRFETQTKICLSISNHHPEHWQPSWSVRTALVALIAF
Sbjct: 61  HGRIQLPAEYPFKPPSFMLLTPNGRFETQTKICLSISNHHPEHWQPSWSVRTALVALIAF 120

Query: 121 MPTNPNGALGSLDYKKEERRVLAIKSREAAPKFGTPERQKLIDEIHEYLLSKAPPAPQPS 180
           MPTNPNGALGSLDYKKEERR LAIKSREAAPKFGTPERQKLIDEIHEY+LSKAPP PQ S
Sbjct: 121 MPTNPNGALGSLDYKKEERRALAIKSREAAPKFGTPERQKLIDEIHEYMLSKAPPVPQLS 180

Query: 181 SPDDSEEKPANKEPDVEVTSEPAELAAAVVEQQNPPISDRIVEEAIPE-ETSINTN-LQQ 240
           +    EE+P N+E + + +S  A +  A  E    P+ DRI+EE   +   S+N N +  
Sbjct: 181 T---CEEQPGNREGEAQASSIDAMVTGA-GEGLPAPVGDRIIEEVQEDLPASMNPNPVVA 240

Query: 241 AAAREAPSDRPAVQL--RTETRVNKPADDRLFTWAAVGLTLAIVVLLLKKFIKASTHGSV 300
             +RE P++   VQL  + ETRV KPADDRLFTWAAVGLT+AI+VLLLKKF+K++ HG+V
Sbjct: 241 GTSREVPANASGVQLQPKPETRVQKPADDRLFTWAAVGLTIAILVLLLKKFMKSNGHGTV 300

Query: 301 FMDES 302
           FMD S
Sbjct: 301 FMDGS 301

[Close]
BLAST of Cp4.1LG08g05100 vs. NCBI nr
Match: gi|255556356|ref|XP_002519212.1| (PREDICTED: ubiquitin-conjugating enzyme E2 32 [Ricinus communis])

HSP 1 Score: 434.9 bits (1117), Expect = 1.2e-118
Identity = 232/310 (74.84%), Postives = 253/310 (81.61%), Query Frame = 1 

Query: 1   MAEDKYNLKNPAVKRILREVKEMQANPSDDFMSLPLEDNIFEWQFAIRGPGDSEFEGGIY 60
           MAEDKYNLKNPAVKRIL+EVKEMQ+NPSDDFMS+PLE+NIFEWQFAIRGP D+EFEGGIY
Sbjct: 1   MAEDKYNLKNPAVKRILQEVKEMQSNPSDDFMSMPLEENIFEWQFAIRGPCDTEFEGGIY 60

Query: 61  HGRIQLPSEYPFKPPSFMLLTPNGRFETQTKICLSISNHHPEHWQPSWSVRTALVALIAF 120
           HGRIQLPSEYPFKPPSF+LLTPNGRFETQTKICLSISNHHPEHWQPSWSVRTALVALIAF
Sbjct: 61  HGRIQLPSEYPFKPPSFLLLTPNGRFETQTKICLSISNHHPEHWQPSWSVRTALVALIAF 120

Query: 121 MPTNPNGALGSLDYKKEERRVLAIKSREAAPKFGTPERQKLIDEIHEYLLSKAPPAPQPS 180
           MPTNPNGALGSLDYKKEERR LAIKSREAAP+FGTPERQKLIDEIH+Y+LSKAPP PQ S
Sbjct: 121 MPTNPNGALGSLDYKKEERRALAIKSREAAPRFGTPERQKLIDEIHQYMLSKAPPVPQLS 180

Query: 181 SPDDSEEKPANKEPDVE-VTSEPAELAA-----AVVEQQNPPISDRIVEEAIPEETSINT 240
              DSEE    KE +   +  E   +AA     AV EQ    + DRIVEE    E  +N 
Sbjct: 181 PSQDSEEHHTGKEDEAHAILQEQGAVAANLPNLAVSEQL--AVGDRIVEEV--PEAPVNV 240

Query: 241 NLQQAAAR---EAPSDRPAVQL--RTETRVNKPADDRLFTWAAVGLTLAIVVLLLKKFIK 300
           N      R   E P+   + QL  R ETRV +PADDRLFTWAAVGLT+AIVVLLLKKF+K
Sbjct: 241 NPGATGVRTSTEIPARASSDQLLHRPETRVQRPADDRLFTWAAVGLTIAIVVLLLKKFLK 300

The following BLAST results are available for this feature:
BLAST of Cp4.1LG08g05100 vs. Swiss-Prot
Match NameE-valueIdentityDescription
UBC32_ARATH4.6e-10765.92Ubiquitin-conjugating enzyme E2 32 OS=Arabidopsis thaliana GN=UBC32 PE=2 SV=1[more]
UB2J1_HUMAN3.4e-5465.07Ubiquitin-conjugating enzyme E2 J1 OS=Homo sapiens GN=UBE2J1 PE=1 SV=2[more]
UB2J1_MOUSE3.4e-5465.07Ubiquitin-conjugating enzyme E2 J1 OS=Mus musculus GN=Ube2j1 PE=1 SV=2[more]
UBC6_YEAST2.8e-3244.44Ubiquitin-conjugating enzyme E2 6 OS=Saccharomyces cerevisiae (strain ATCC 20450... [more]
UBC6_ASHGO2.8e-3243.80Ubiquitin-conjugating enzyme E2 6 OS=Ashbya gossypii (strain ATCC 10895 / CBS 10... [more]
BLAST of Cp4.1LG08g05100 vs. TrEMBL
Match NameE-valueIdentityDescription
A0A0A0L954_CUCSA3.5e-13884.54Uncharacterized protein OS=Cucumis sativus GN=Csa_3G166270 PE=4 SV=1[more]
V4RY15_9ROSI1.2e-12276.07Uncharacterized protein OS=Citrus clementina GN=CICLE_v10005518mg PE=4 SV=1[more]
A0A067FWY2_CITSI3.5e-12275.74Uncharacterized protein OS=Citrus sinensis GN=CISIN_1g020053mg PE=4 SV=1[more]
B9RZP3_RICCO8.1e-11974.84Non-canonical ubiquitin conjugating enzyme, putative OS=Ricinus communis GN=RCOM... [more]
W9RG08_9ROSA1.5e-11771.85Ubiquitin-conjugating enzyme E2 32 OS=Morus notabilis GN=L484_004205 PE=4 SV=1[more]
BLAST of Cp4.1LG08g05100 vs. TAIR10
Match NameE-valueIdentityDescription
AT3G17000.12.6e-10865.92 ubiquitin-conjugating enzyme 32[more]
AT5G50430.12.9e-2733.16 ubiquitin-conjugating enzyme 33[more]
AT1G17280.15.0e-2737.10 ubiquitin-conjugating enzyme 34[more]
AT4G27960.24.0e-1634.19 ubiquitin conjugating enzyme 9[more]
AT3G08690.16.8e-1634.19 ubiquitin-conjugating enzyme 11[more]
BLAST of Cp4.1LG08g05100 vs. NCBI nr
Match NameE-valueIdentityDescription
gi|659076938|ref|XP_008438945.1|2.9e-13884.21PREDICTED: ubiquitin-conjugating enzyme E2 32 [Cucumis melo][more]
gi|449432914|ref|XP_004134243.1|5.0e-13884.54PREDICTED: ubiquitin-conjugating enzyme E2 32 [Cucumis sativus][more]
gi|567852467|ref|XP_006419397.1|1.7e-12276.07hypothetical protein CICLE_v10005518mg [Citrus clementina][more]
gi|568871343|ref|XP_006488848.1|5.0e-12275.74PREDICTED: ubiquitin-conjugating enzyme E2 32 [Citrus sinensis][more]
gi|255556356|ref|XP_002519212.1|1.2e-11874.84PREDICTED: ubiquitin-conjugating enzyme E2 32 [Ricinus communis][more]
Annotated Terms
The following terms have been associated with this gene:
Vocabulary: INTERPRO
TermDefinition
IPR016135UBQ-conjugating_enzyme/RWD
IPR000608UBQ-conjugat_E2
GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0016036 cellular response to phosphate starvation
biological_process GO:0008150 biological_process
biological_process GO:0090304 nucleic acid metabolic process
biological_process GO:0033554 cellular response to stress
biological_process GO:0031668 cellular response to extracellular stimulus
biological_process GO:0044255 cellular lipid metabolic process
biological_process GO:0044249 cellular biosynthetic process
biological_process GO:0044712 single-organism catabolic process
biological_process GO:0042631 cellular response to water deprivation
biological_process GO:0006511 ubiquitin-dependent protein catabolic process
biological_process GO:1901576 organic substance biosynthetic process
biological_process GO:0042221 response to chemical
biological_process GO:0009407 toxin catabolic process
biological_process GO:0051788 response to misfolded protein
biological_process GO:0016567 protein ubiquitination
biological_process GO:0043161 proteasome-mediated ubiquitin-dependent protein catabolic process
biological_process GO:0080129 proteasome core complex assembly
biological_process GO:0006301 postreplication repair
biological_process GO:0045892 negative regulation of transcription, DNA-templated
biological_process GO:0019375 galactolipid biosynthetic process
biological_process GO:0006635 fatty acid beta-oxidation
cellular_component GO:0044444 cytoplasmic part
cellular_component GO:0016021 integral component of membrane
cellular_component GO:0005575 cellular_component
cellular_component GO:0048471 perinuclear region of cytoplasm
cellular_component GO:0016020 membrane
cellular_component GO:0005783 endoplasmic reticulum
molecular_function GO:0003674 molecular_function
molecular_function GO:0016874 ligase activity
molecular_function GO:0003824 catalytic activity
molecular_function GO:0004842 ubiquitin-protein transferase activity
Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Cp4.1LG08g05100.1Cp4.1LG08g05100.1mRNA


InterPro
Analysis Name: InterPro Annotations of Cucurbita pepo
Date Performed: 2017-12-02
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR000608Ubiquitin-conjugating enzyme E2PFAMPF00179UQ_concoord: 15..126
score: 2.8
IPR000608Ubiquitin-conjugating enzyme E2PROFILEPS50127UBIQUITIN_CONJUGAT_2coord: 14..126
score: 24
IPR016135Ubiquitin-conjugating enzyme/RWD-likeGENE3DG3DSA:3.10.110.10coord: 7..188
score: 2.6
IPR016135Ubiquitin-conjugating enzyme/RWD-likeunknownSSF54495UBC-likecoord: 12..135
score: 2.88
NoneNo IPR availablePANTHERPTHR24067UBIQUITIN-CONJUGATING ENZYME E2coord: 1..148
score: 9.8
NoneNo IPR availablePANTHERPTHR24067:SF54UBIQUITIN-CONJUGATING ENZYME E2 J1coord: 1..148
score: 9.8
NoneNo IPR availableSMARTSM00212ubc_7coord: 14..174
score: 8.1

Synteny
The following gene(s) are orthologous to this gene:
GeneOrthologueOrganismBlock
Cp4.1LG08g05100Cucsa.252590Cucumber (Gy14) v1cgycpeB0687
Cp4.1LG08g05100CmaCh14G015930Cucurbita maxima (Rimu)cmacpeB292
Cp4.1LG08g05100CmaCh06G015600Cucurbita maxima (Rimu)cmacpeB853
Cp4.1LG08g05100CmoCh06G015570Cucurbita moschata (Rifu)cmocpeB796
Cp4.1LG08g05100CmoCh14G016240Cucurbita moschata (Rifu)cmocpeB254
Cp4.1LG08g05100Cla021788Watermelon (97103) v1cpewmB845
Cp4.1LG08g05100Csa3G166270Cucumber (Chinese Long) v2cpecuB859
Cp4.1LG08g05100MELO3C006710Melon (DHL92) v3.5.1cpemeB813
Cp4.1LG08g05100ClCG05G006510Watermelon (Charleston Gray)cpewcgB787
Cp4.1LG08g05100CSPI03G14930Wild cucumber (PI 183967)cpecpiB861
Cp4.1LG08g05100Lsi05G015870Bottle gourd (USVL1VR-Ls)cpelsiB718
Cp4.1LG08g05100MELO3C006710.2Melon (DHL92) v3.6.1cpemedB951
Cp4.1LG08g05100CsaV3_3G015120Cucumber (Chinese Long) v3cpecucB1056
Cp4.1LG08g05100Bhi01G000775Wax gourdcpewgoB1110
Cp4.1LG08g05100CsGy3G015040Cucumber (Gy14) v2cgybcpeB454
Cp4.1LG08g05100Carg23738Silver-seed gourdcarcpeB1321
Cp4.1LG08g05100Carg19627Silver-seed gourdcarcpeB0396
The following gene(s) are paralogous to this gene:
GeneParalogueOrganismBlock
Cp4.1LG08g05100Cp4.1LG03g11700Cucurbita pepo (Zucchini)cpecpeB482
The following block(s) are covering this gene:
GeneOrganismBlock
Cp4.1LG08g05100Silver-seed gourdcarcpeB0109
Cp4.1LG08g05100Silver-seed gourdcarcpeB0987
Cp4.1LG08g05100Wax gourdcpewgoB1102
Cp4.1LG08g05100Cucurbita pepo (Zucchini)cpecpeB194
Cp4.1LG08g05100Cucurbita pepo (Zucchini)cpecpeB343
Cp4.1LG08g05100Cucurbita maxima (Rimu)cmacpeB412
Cp4.1LG08g05100Cucurbita maxima (Rimu)cmacpeB931
Cp4.1LG08g05100Cucurbita moschata (Rifu)cmocpeB373
Cp4.1LG08g05100Cucurbita moschata (Rifu)cmocpeB866
Cp4.1LG08g05100Bottle gourd (USVL1VR-Ls)cpelsiB697
Cp4.1LG08g05100Watermelon (Charleston Gray)cpewcgB788
Cp4.1LG08g05100Watermelon (97103) v1cpewmB853
Cp4.1LG08g05100Melon (DHL92) v3.5.1cpemeB793