Cucsa.250610 (gene) Cucumber (Gy14) v1

BLAST of Cucsa.250610 vs. Swiss-Prot
Match: PP139_ARATH (Pentatricopeptide repeat-containing protein At2g01390 OS=Arabidopsis thaliana GN=At2g01390/At2g01380 PE=2 SV=2)

HSP 1 Score: 551.2 bits (1419), Expect = 1.4e-155
Identity = 288/558 (51.61%), Postives = 383/558 (68.64%), Query Frame = 1

Query: 29  SSKCLHSLHQYKRDKPISRFSRQSRKGTKVAKKEEVIPRLYTRDTVRNICNILRNCSWAS 88
           S K LHSL + K     +   R S+K   V  +    P +YTRD V NI NIL+  +W S
Sbjct: 20  SVKLLHSLPRLKP----TNSKRFSQKPKLVKTQTLPDPSVYTRDIVSNIYNILKYSNWDS 79

Query: 89  AQKHLEMLPIRWDSYLINQVLKTHPPLEKTWLFFNWASTLQVFKHDQYTYTTMLDIFGEA 148
           AQ+ L  L +RWDS++IN+VLK HPP++K WLFFNWA+ ++ FKHD +TYTTMLDIFGEA
Sbjct: 80  AQEQLPHLGVRWDSHIINRVLKAHPPMQKAWLFFNWAAQIKGFKHDHFTYTTMLDIFGEA 139

Query: 149 GRISSMNYVFQQMKEKGIKIDAVTYTSLMHWRSNSGDVDGAIKVWKEMKANGCHPTVVSY 208
           GRI SM  VF  MKEKG+ ID VTYTSL+HW S+SGDVDGA+++W+EM+ NGC PTVVSY
Sbjct: 140 GRIQSMYSVFHLMKEKGVLIDTVTYTSLIHWVSSSGDVDGAMRLWEEMRDNGCEPTVVSY 199

Query: 209 TAYIKILLDNGQINEATATYKKMLQSGLSPNCCTYTILMEYLIGEGKCKEALDIFSKMQD 268
           TAY+K+L  +G++ EAT  YK+ML+S +SPNC TYT+LMEYL+  GKC+EALDIF KMQ+
Sbjct: 200 TAYMKMLFADGRVEEATEVYKEMLRSRVSPNCHTYTVLMEYLVATGKCEEALDIFFKMQE 259

Query: 269 AGVYPDKAACNILIQKCCKSGERLVMTQILEFMKENRFVLRYPVFVEAHETLKSCSVSYA 328
            GV PDKAACNILI K  K GE   MT++L +MKEN  VLRYP+FVEA ETLK+   S  
Sbjct: 260 IGVQPDKAACNILIAKALKFGETSFMTRVLVYMKENGVVLRYPIFVEALETLKAAGESDD 319

Query: 329 LLKQVNPHMEIESISKGEVVDVSTGSNTVPPNVDNE--LLAMLLKDNKLTAVDHMLIGIV 388
           LL++VN H+ +ES+   ++ +  T       N D+   + ++LL    L AVD +L  + 
Sbjct: 320 LLREVNSHISVESLCSSDIDETPTAEVNDTKNSDDSRVISSVLLMKQNLVAVDILLNQMR 379

Query: 389 DKNIQLDSSIIYSIIEVNCKSNRPNSALLAFDYCLKNSVNIKRKLYLDLIGILIRSSIYP 448
           D+NI+LDS ++ +IIE NC   R   A LAFDY L+  +++K+  YL LIG  +RS+  P
Sbjct: 380 DRNIKLDSFVVSAIIETNCDRCRTEGASLAFDYSLEMGIHLKKSAYLALIGNFLRSNELP 439

Query: 449 KLLEIVQEMYTQGHCLGLYHATLILYSLGKAGKPQYARKVFNMLPEELKCTATYTALVDG 508
           K++E+V+EM    H LG Y   ++++ LG   +P+ A  VF++LP++ K  A YTAL+D 
Sbjct: 440 KVIEVVKEMVKAQHSLGCYQGAMLIHRLGFGRRPRLAADVFDLLPDDQKGVAAYTALMDV 499

Query: 509 YFSAGSSGKGLKIFETMRKKGFTPSLGTYNVLLNGLAKNGR-GVELNIYRREKKSFEISH 568
           Y SAGS  K +KI   MR++   PSLGTY+VLL+GL K      E+ + R+EKKS   S 
Sbjct: 500 YISAGSPEKAMKILREMREREIMPSLGTYDVLLSGLEKTSDFQKEVALLRKEKKSLVASA 559

Query: 569 HSRLNTILDDERICDLLF 584
             R N  ++D +ICDLLF
Sbjct: 560 RFRENVHVED-KICDLLF 572

BLAST of Cucsa.250610 vs. Swiss-Prot
Match: PP123_ARATH (Pentatricopeptide repeat-containing protein At1g74750 OS=Arabidopsis thaliana GN=At1g74750 PE=2 SV=1)

HSP 1 Score: 123.6 bits (309), Expect = 7.0e-27
Identity = 82/260 (31.54%), Postives = 124/260 (47.69%), Query Frame = 1

Query: 29  SSKCLHSLHQYKRDKPISR---FSRQSRKGTKVAKKEEVIPRLYTRD--TVRNICNILRN 88
           S  C +S+H       IS    F + SR+  KV  +    PR +      V N+ +ILR 
Sbjct: 247 SGGCDYSVHSSDDRTIISSVEGFGKPSREMMKVTPRTAPTPRQHCNPGYVVENVSSILRR 306

Query: 89  CSWA-SAQKHLEMLPIRWDSYLINQVLKTHPPLEKTWLFFNWASTLQVFKHDQYTYTTML 148
             W  +A++ L     R D+Y  NQVLK          FF W      FKHD +TYTTM+
Sbjct: 307 FKWGHAAEEALHNFGFRMDAYQANQVLKQMDNYANALGFFYWLKRQPGFKHDGHTYTTMV 366

Query: 149 DIFGEAGRISSMNYVFQQMKEKGIKIDAVTYTSLMHWRSNSGDVDGAIKVWKEMKANGCH 208
              G A +   +N +  +M   G K + VTY  L+H    +  +  A+ V+ +M+  GC 
Sbjct: 367 GNLGRAKQFGEINKLLDEMVRDGCKPNTVTYNRLIHSYGRANYLKEAMNVFNQMQEAGCE 426

Query: 209 PTVVSYTAYIKILLDNGQINEATATYKKMLQSGLSPNCCTYTILMEYLIGEGKCKEALDI 268
           P  V+Y   I I    G ++ A   Y++M ++GLSP+  TY++++  L   G    A  +
Sbjct: 427 PDRVTYCTLIDIHAKAGFLDIAMDMYQRMQEAGLSPDTFTYSVIINCLGKAGHLPAAHRL 486

Query: 269 FSKMQDAGVYPDKAACNILI 283
           F +M   G  P+    NI+I
Sbjct: 487 FCEMVGQGCTPNLVTFNIMI 506

HSP 2 Score: 84.0 bits (206), Expect = 6.2e-15
Identity = 46/156 (29.49%), Postives = 82/156 (52.56%), Query Frame = 1

Query: 134 DQYTYTTMLDIFGEAGRISSMNYVFQQMKEKGIKIDAVTYTSLMHWRSNSGDVDGAIKVW 193
           D+ TY T++DI  +AG +     ++Q+M+E G+  D  TY+ +++    +G +  A +++
Sbjct: 428 DRVTYCTLIDIHAKAGFLDIAMDMYQRMQEAGLSPDTFTYSVIINCLGKAGHLPAAHRLF 487

Query: 194 KEMKANGCHPTVVSYTAYIKILLDNGQINEATATYKKMLQSGLSPNCCTYTILMEYLIGE 253
            EM   GC P +V++   I +         A   Y+ M  +G  P+  TY+I+ME L   
Sbjct: 488 CEMVGQGCTPNLVTFNIMIALHAKARNYETALKLYRDMQNAGFQPDKVTYSIVMEVLGHC 547

Query: 254 GKCKEALDIFSKMQDAGVYPDKAACNILIQKCCKSG 290
           G  +EA  +F++MQ     PD+    +L+    K+G
Sbjct: 548 GFLEEAEGVFAEMQRKNWVPDEPVYGLLVDLWGKAG 583

HSP 3 Score: 59.7 bits (143), Expect = 1.2e-07
Identity = 36/149 (24.16%), Postives = 69/149 (46.31%), Query Frame = 1

Query: 137 TYTTMLDIFGEAGRISSMNYVFQQMKEKGIKIDAVTYTSLMHWRSNSGDVDGAIKVWKEM 196
           T+  M+ +  +A    +   +++ M+  G + D VTY+ +M    + G ++ A  V+ EM
Sbjct: 501 TFNIMIALHAKARNYETALKLYRDMQNAGFQPDKVTYSIVMEVLGHCGFLEEAEGVFAEM 560

Query: 197 KANGCHPTVVSYTAYIKILLDNGQINEATATYKKMLQSGLSPNCCTYTILMEYLIGEGKC 256
           +     P    Y   + +    G +++A   Y+ MLQ+GL PN  T   L+   +   + 
Sbjct: 561 QRKNWVPDEPVYGLLVDLWGKAGNVDKAWQWYQAMLQAGLRPNVPTCNSLLSTFLRVHRM 620

Query: 257 KEALDIFSKMQDAGVYPDKAACNILIQKC 286
            EA ++   M   G++P      +L+  C
Sbjct: 621 SEAYNLLQSMLALGLHPSLQTYTLLLSCC 649

HSP 4 Score: 45.4 bits (106), Expect = 2.4e-03
Identity = 28/97 (28.87%), Postives = 46/97 (47.42%), Query Frame = 1

Query: 207 SYTAYIKILLDNGQINEATATYKKMLQSGLSPNCCTYTILMEYLIGEGKCKEALDIFSKM 266
           +YT  +  L    Q  E      +M++ G  PN  TY  L+         KEA+++F++M
Sbjct: 361 TYTTMVGNLGRAKQFGEINKLLDEMVRDGCKPNTVTYNRLIHSYGRANYLKEAMNVFNQM 420

Query: 267 QDAGVYPDKAACNILIQKCCKSGERLVMTQILEFMKE 304
           Q+AG  PD+     LI    K+G   +   + + M+E
Sbjct: 421 QEAGCEPDRVTYCTLIDIHAKAGFLDIAMDMYQRMQE 457

BLAST of Cucsa.250610 vs. Swiss-Prot
Match: PP445_ARATH (Pentatricopeptide repeat-containing protein At5g65560 OS=Arabidopsis thaliana GN=At5g65560 PE=2 SV=1)

HSP 1 Score: 120.6 bits (301), Expect = 6.0e-26
Identity = 101/424 (23.82%), Postives = 192/424 (45.28%), Query Frame = 1

Query: 134 DQYTYTTMLDIFGEAGRISSMNYVFQQMKEKGIKIDAVTYTSLMHWRSNSGDVDGAIKVW 193
           DQ+TYT+M+D   ++ R+     +F  +++KG+  + V YT+L+     +G VD A  + 
Sbjct: 496 DQWTYTSMIDSLCKSKRVEEACDLFDSLEQKGVNPNVVMYTALIDGYCKAGKVDEAHLML 555

Query: 194 KEMKANGCHPTVVSYTAYIKILLDNGQINEATATYKKMLQSGLSPNCCTYTILMEYLIGE 253
           ++M +  C P  +++ A I  L  +G++ EAT   +KM++ GL P   T TIL+  L+ +
Sbjct: 556 EKMLSKNCLPNSLTFNALIHGLCADGKLKEATLLEEKMVKIGLQPTVSTDTILIHRLLKD 615

Query: 254 GKCKEALDIFSKMQDAGVYPDKAACNILIQKCCKSGERLVMTQILEFMKENRF---VLRY 313
           G    A   F +M  +G  PD       IQ  C+ G  L    ++  M+EN     +  Y
Sbjct: 616 GDFDHAYSRFQQMLSSGTKPDAHTYTTFIQTYCREGRLLDAEDMMAKMRENGVSPDLFTY 675

Query: 314 PVFVEAHETLKSCSVSYALLKQV-------NPHMEIESISKGEVVDVSTGSNTVPPNVDN 373
              ++ +  L   + ++ +LK++       + H  +  I    ++++  G        + 
Sbjct: 676 SSLIKGYGDLGQTNFAFDVLKRMRDTGCEPSQHTFLSLIK--HLLEMKYGKQ---KGSEP 735

Query: 374 ELLAMLLKDNKLTAVDHMLIGIVDKNIQLDSSIIYSIIEVNCKSNRPNSALLAFDYCLKN 433
           EL AM       T V+ +L  +V+ ++  ++     +I   C+      A   FD+  +N
Sbjct: 736 ELCAMSNMMEFDTVVE-LLEKMVEHSVTPNAKSYEKLILGICEVGNLRVAEKVFDHMQRN 795

Query: 434 -SVNIKRKLYLDLIGILIRSSIYPKLLEIVQEMYTQGHCLGLYHATLILYSLGKAGKPQY 493
             ++    ++  L+    +   + +  ++V +M   GH   L    +++  L K G+ + 
Sbjct: 796 EGISPSELVFNALLSCCCKLKKHNEAAKVVDDMICVGHLPQLESCKVLICGLYKKGEKER 855

Query: 494 ARKVFNMLPEELKC-----TATYTALVDGYFSAGSSGKGLKIFETMRKKGFTPSLGTYNV 542
              VF  L   L+C        +  ++DG    G      ++F  M K G   S  TY++
Sbjct: 856 GTSVFQNL---LQCGYYEDELAWKIIIDGVGKQGLVEAFYELFNVMEKNGCKFSSQTYSL 910

HSP 2 Score: 114.0 bits (284), Expect = 5.6e-24
Identity = 107/423 (25.30%), Postives = 180/423 (42.55%), Query Frame = 1

Query: 136 YTYTTMLDIFGEAGRISSMNYVFQQMKEKGIKIDAVTYTSLMHWRSNSGDVDGAIKVWKE 195
           YTY  M++ + + G +   N    ++ E G+  D  TYTSL+       D+D A KV+ E
Sbjct: 219 YTYNKMVNGYCKLGNVEEANQYVSKIVEAGLDPDFFTYTSLIMGYCQRKDLDSAFKVFNE 278

Query: 196 MKANGCHPTVVSYTAYIKILLDNGQINEATATYKKMLQSGLSPNCCTYTILMEYLIGEGK 255
           M   GC    V+YT  I  L    +I+EA   + KM      P   TYT+L++ L G  +
Sbjct: 279 MPLKGCRRNEVAYTHLIHGLCVARRIDEAMDLFVKMKDDECFPTVRTYTVLIKSLCGSER 338

Query: 256 CKEALDIFSKMQDAGVYPDKAACNILIQKCCKSGERLVMTQILEFMKENRFVLRYPVFVE 315
             EAL++  +M++ G+ P+     +LI   C   +     ++L  M E            
Sbjct: 339 KSEALNLVKEMEETGIKPNIHTYTVLIDSLCSQCKFEKARELLGQMLE------------ 398

Query: 316 AHETLKSCSVSYALLKQVNPHMEIESISKGEVVDVSTGSNTVPPNVD--NELLAMLLKDN 375
             + L    ++Y  L  +N + +   I     V     S  + PN    NEL+    K N
Sbjct: 399 --KGLMPNVITYNAL--INGYCKRGMIEDAVDVVELMESRKLSPNTRTYNELIKGYCKSN 458

Query: 376 KLTAVDHMLIGIVDKNIQ---LDSSIIY-SIIEVNCKSNRPNSALLAFDYCLKNSVNIKR 435
                 H  +G+++K ++   L   + Y S+I+  C+S   +SA           +   +
Sbjct: 459 V-----HKAMGVLNKMLERKVLPDVVTYNSLIDGQCRSGNFDSAYRLLSLMNDRGLVPDQ 518

Query: 436 KLYLDLIGILIRSSIYPKLLEIVQEMYTQGHCLGLYHATLILYSLGKAGKPQYARKVF-- 495
             Y  +I  L +S    +  ++   +  +G    +   T ++    KAGK   A  +   
Sbjct: 519 WTYTSMIDSLCKSKRVEEACDLFDSLEQKGVNPNVVMYTALIDGYCKAGKVDEAHLMLEK 578

Query: 496 ----NMLPEELKCTATYTALVDGYFSAGSSGKGLKIFETMRKKGFTPSLGTYNVLLNGLA 547
               N LP  L    T+ AL+ G  + G   +   + E M K G  P++ T  +L++ L 
Sbjct: 579 MLSKNCLPNSL----TFNALIHGLCADGKLKEATLLEEKMVKIGLQPTVSTDTILIHRLL 616

HSP 3 Score: 80.9 bits (198), Expect = 5.2e-14
Identity = 99/415 (23.86%), Postives = 164/415 (39.52%), Query Frame = 1

Query: 137 TYTTMLDIFGEAGRISSMNYVFQQMKEKGIKIDAVTYTSLMHWRSNSGDVDGAIKVWKEM 196
           TYT ++     + R S    + ++M+E GIK +  TYT L+    +    + A ++  +M
Sbjct: 325 TYTVLIKSLCGSERKSEALNLVKEMEETGIKPNIHTYTVLIDSLCSQCKFEKARELLGQM 384

Query: 197 KANGCHPTVVSYTAYIKILLDNGQINEATATYKKMLQSGLSPNCCTYTILMEYLIGEGKC 256
              G  P V++Y A I      G I +A    + M    LSPN  TY  L+     +G C
Sbjct: 385 LEKGLMPNVITYNALINGYCKRGMIEDAVDVVELMESRKLSPNTRTYNELI-----KGYC 444

Query: 257 K----EALDIFSKMQDAGVYPDKAACNILIQKCCKSGERLVMTQILEFMKENRFVLRYPV 316
           K    +A+ + +KM +  V PD    N LI   C+SG      ++L  M +   V     
Sbjct: 445 KSNVHKAMGVLNKMLERKVLPDVVTYNSLIDGQCRSGNFDSAYRLLSLMNDRGLVPDQWT 504

Query: 317 FVEAHETL-KSCSVSYALLKQVNPHMEIESISKGEVVDVSTGSNTVPPNV--DNELLAML 376
           +    ++L KS  V  A                 ++ D S     V PNV     L+   
Sbjct: 505 YTSMIDSLCKSKRVEEAC----------------DLFD-SLEQKGVNPNVVMYTALIDGY 564

Query: 377 LKDNKLTAVDHMLIGIVDKNIQLDSSIIYSIIEVNCKSNRPNSALLAFDYCLKNSVNIKR 436
            K  K+     ML  ++ KN   +S    ++I   C   +   A L  +  +K  +    
Sbjct: 565 CKAGKVDEAHLMLEKMLSKNCLPNSLTFNALIHGLCADGKLKEATLLEEKMVKIGLQPTV 624

Query: 437 KLYLDLIGILIRSSIYPKLLEIVQEMYTQGHCLGLYHATLILYSLGKAGKPQYARKVFNM 496
                LI  L++   +       Q+M + G     +  T  + +  + G+   A  +   
Sbjct: 625 STDTILIHRLLKDGDFDHAYSRFQQMLSSGTKPDAHTYTTFIQTYCREGRLLDAEDMMAK 684

Query: 497 LPEE--LKCTATYTALVDGYFSAGSSGKGLKIFETMRKKGFTPSLGTYNVLLNGL 543
           + E        TY++L+ GY   G +     + + MR  G  PS  T+  L+  L
Sbjct: 685 MRENGVSPDLFTYSSLIKGYGDLGQTNFAFDVLKRMRDTGCEPSQHTFLSLIKHL 717

BLAST of Cucsa.250610 vs. Swiss-Prot
Match: PP344_ARATH (Pentatricopeptide repeat-containing protein At4g31850, chloroplastic OS=Arabidopsis thaliana GN=PGR3 PE=1 SV=1)

HSP 1 Score: 120.2 bits (300), Expect = 7.8e-26
Identity = 112/422 (26.54%), Postives = 190/422 (45.02%), Query Frame = 1

Query: 134 DQYTYTTMLDIFGEAGRISSMNYVFQQMKEKGIKIDAVTYTSLMHWRSNSGDVDGAIKVW 193
           D  TY  M+  + + G I     +  +M E G + D +   SL++    +  VD A K++
Sbjct: 502 DSVTYNMMMKCYSKVGEIDEAIKLLSEMMENGCEPDVIVVNSLINTLYKADRVDEAWKMF 561

Query: 194 KEMKANGCHPTVVSYTAYIKILLDNGQINEATATYKKMLQSGLSPNCCTYTILMEYLIGE 253
             MK     PTVV+Y   +  L  NG+I EA   ++ M+Q G  PN  T+  L + L   
Sbjct: 562 MRMKEMKLKPTVVTYNTLLAGLGKNGKIQEAIELFEGMVQKGCPPNTITFNTLFDCLCKN 621

Query: 254 GKCKEALDIFSKMQDAGVYPDKAACNILIQKCCKSGERLVMTQILEFMKENRFVLRYPVF 313
            +   AL +  KM D G  PD    N +I    K+G+          MK+    L YP F
Sbjct: 622 DEVTLALKMLFKMMDMGCVPDVFTYNTIIFGLVKNGQVKEAMCFFHQMKK----LVYPDF 681

Query: 314 VEAHETLKSCSVSYALLKQVNPHMEIESISKGEVVDVSTGSNTVPPNVDNELLAMLLKDN 373
           V    TL  C++   ++K        + I+          +N    ++   +LA    DN
Sbjct: 682 V----TL--CTLLPGVVKASLIEDAYKIITNFLYNCADQPANLFWEDLIGSILAEAGIDN 741

Query: 374 KLTAVDHMLIGIVDKNIQLDSSIIYSIIEVNCKSNRPNSALLAFDYCLKN-SVNIKRKLY 433
            ++  + ++   + ++     SI+  II  +CK N  + A   F+   K+  V  K   Y
Sbjct: 742 AVSFSERLVANGICRD---GDSILVPIIRYSCKHNNVSGARTLFEKFTKDLGVQPKLPTY 801

Query: 434 LDLIGILIRSSIYPKLLEIVQEMYTQGHCLG----LYHATLILYSLGKAGKPQYARKVF- 493
             LIG L+ +     ++EI Q+++ Q    G    +     +L + GK+GK     +++ 
Sbjct: 802 NLLIGGLLEAD----MIEIAQDVFLQVKSTGCIPDVATYNFLLDAYGKSGKIDELFELYK 861

Query: 494 NMLPEELKC-TATYTALVDGYFSAGSSGKGLKI-FETMRKKGFTPSLGTYNVLLNGLAKN 548
            M   E +  T T+  ++ G   AG+    L + ++ M  + F+P+  TY  L++GL+K+
Sbjct: 862 EMSTHECEANTITHNIVISGLVKAGNVDDALDLYYDLMSDRDFSPTACTYGPLIDGLSKS 906

HSP 2 Score: 114.0 bits (284), Expect = 5.6e-24
Identity = 108/434 (24.88%), Postives = 184/434 (42.40%), Query Frame = 1

Query: 129 QVFKHDQYTYTTMLDIFGEAGRISSMNYVFQQMKEKGIKIDAVTYTSLMHWRSNSGDVDG 188
           ++ K D  TY T+       G +    Y  ++M+E G  ++A +Y  L+H    S     
Sbjct: 147 RIIKRDTNTYLTIFKSLSVKGGLKQAPYALRKMREFGFVLNAYSYNGLIHLLLKSRFCTE 206

Query: 189 AIKVWKEMKANGCHPTVVSYTAYIKILLDNGQINEATATYKKMLQSGLSPNCCTYTILME 248
           A++V++ M   G  P++ +Y++ +  L     I+      K+M   GL PN  T+TI + 
Sbjct: 207 AMEVYRRMILEGFRPSLQTYSSLMVGLGKRRDIDSVMGLLKEMETLGLKPNVYTFTICIR 266

Query: 249 YLIGEGKCKEALDIFSKMQDAGVYPDKAACNILIQKCCKSGERLVMTQILEFMKENRFVL 308
            L   GK  EA +I  +M D G  PD     +LI   C + +     ++ E MK  R   
Sbjct: 267 VLGRAGKINEAYEILKRMDDEGCGPDVVTYTVLIDALCTARKLDCAKEVFEKMKTGRH-- 326

Query: 309 RYPVFVEAHETLKSCSVSY-ALLKQVNPHMEIESISK--------GEVVDVSTGSNTVPP 368
                       K   V+Y  LL + + + +++S+ +        G V DV T +  V  
Sbjct: 327 ------------KPDRVTYITLLDRFSDNRDLDSVKQFWSEMEKDGHVPDVVTFTILVD- 386

Query: 369 NVDNELLAMLLKDNKLTAVDHMLI----GIVDKNIQLDSSIIYSIIEVNCKSNRPNSALL 428
                  A+    N   A D + +    GI+  N+   +++I  ++ V    +R + AL 
Sbjct: 387 -------ALCKAGNFGEAFDTLDVMRDQGIL-PNLHTYNTLICGLLRV----HRLDDALE 446

Query: 429 AFDYCLKNSVNIKRKLYLDLIGILIRSSIYPKLLEIVQEMYTQGHCLGLYHATLILYSLG 488
            F       V      Y+  I    +S      LE  ++M T+G    +      LYSL 
Sbjct: 447 LFGNMESLGVKPTAYTYIVFIDYYGKSGDSVSALETFEKMKTKGIAPNIVACNASLYSLA 506

Query: 489 KAGKPQYARKVFNMLPE--ELKCTATYTALVDGYFSAGSSGKGLKIFETMRKKGFTPSLG 548
           KAG+ + A+++F  L +   +  + TY  ++  Y   G   + +K+   M + G  P + 
Sbjct: 507 KAGRDREAKQIFYGLKDIGLVPDSVTYNMMMKCYSKVGEIDEAIKLLSEMMENGCEPDVI 553

HSP 3 Score: 99.4 bits (246), Expect = 1.4e-19
Identity = 102/450 (22.67%), Postives = 183/450 (40.67%), Query Frame = 1

Query: 132 KHDQYTYTTMLDIFGEAGRISSMNYVFQQMKEKGIKIDAVTYTSLMHWRSNSGDVDGAIK 191
           K + YT+T  + + G AG+I+    + ++M ++G   D VTYT L+     +  +D A +
Sbjct: 255 KPNVYTFTICIRVLGRAGKINEAYEILKRMDDEGCGPDVVTYTVLIDALCTARKLDCAKE 314

Query: 192 VWKEMKANGCHPTVVSYTAYIKILLDNGQINEATATYKKMLQSGLSPNCCTYTILMEYLI 251
           V+++MK     P  V+Y   +    DN  ++     + +M + G  P+  T+TIL++ L 
Sbjct: 315 VFEKMKTGRHKPDRVTYITLLDRFSDNRDLDSVKQFWSEMEKDGHVPDVVTFTILVDALC 374

Query: 252 GEGKCKEALDIFSKMQDAGVYPDKAACNILI---QKCCKSGERLVMTQILEFMKENRFVL 311
             G   EA D    M+D G+ P+    N LI    +  +  + L +   +E +       
Sbjct: 375 KAGNFGEAFDTLDVMRDQGILPNLHTYNTLICGLLRVHRLDDALELFGNMESLGVKPTAY 434

Query: 312 RYPVFVEAHETLKSCSVSYALLKQVNPHMEIESISKGEVVDVSTGSNTVPPNV--DNELL 371
            Y VF++ +             K  +    +E+  K +       +  + PN+   N  L
Sbjct: 435 TYIVFIDYYG------------KSGDSVSALETFEKMK-------TKGIAPNIVACNASL 494

Query: 372 AMLLKDNKLTAVDHMLIGIVDKNIQLDSSIIYSIIEVNCKSNRPNSALLAFDYCLKNSVN 431
             L K  +      +  G+ D  +  DS     +++   K    + A+      ++N   
Sbjct: 495 YSLAKAGRDREAKQIFYGLKDIGLVPDSVTYNMMMKCYSKVGEIDEAIKLLSEMMENGCE 554

Query: 432 IKRKLYLDLIGILIRSSIYPKLLEIVQEMYTQGHCLGLYHATLILYSLGKAGKPQYARKV 491
               +   LI  L ++    +  ++   M        +     +L  LGK GK Q A ++
Sbjct: 555 PDVIVVNSLINTLYKADRVDEAWKMFMRMKEMKLKPTVVTYNTLLAGLGKNGKIQEAIEL 614

Query: 492 FNMLPEELKC---TATYTALVDGYFSAGSSGKGLKIFETMRKKGFTPSLGTYNVLLNGLA 551
           F  + ++  C   T T+  L D           LK+   M   G  P + TYN ++ GL 
Sbjct: 615 FEGMVQK-GCPPNTITFNTLFDCLCKNDEVTLALKMLFKMMDMGCVPDVFTYNTIIFGLV 674

Query: 552 KNGRGVELNIYRREKKSFEISHHSRLNTIL 574
           KNG+  E   +  + K         L T+L
Sbjct: 675 KNGQVKEAMCFFHQMKKLVYPDFVTLCTLL 684

HSP 4 Score: 85.9 bits (211), Expect = 1.6e-15
Identity = 108/486 (22.22%), Postives = 202/486 (41.56%), Query Frame = 1

Query: 104  LINQVLKTHPPLEKTWLFFNWASTLQVFKHDQYTYTTMLDIFGEAGRISSMNYVFQQMKE 163
            LIN + K    +++ W  F     +++ K    TY T+L   G+ G+I     +F+ M +
Sbjct: 544  LINTLYKADR-VDEAWKMFMRMKEMKL-KPTVVTYNTLLAGLGKNGKIQEAIELFEGMVQ 603

Query: 164  KGIKIDAVTYTSLMHWRSNSGDVDGAIKVWKEMKANGCHPTVVSYTAYIKILLDNGQINE 223
            KG   + +T+ +L      + +V  A+K+  +M   GC P V +Y   I  L+ NGQ+ E
Sbjct: 604  KGCPPNTITFNTLFDCLCKNDEVTLALKMLFKMMDMGCVPDVFTYNTIIFGLVKNGQVKE 663

Query: 224  ATATYKKMLQSGLSPNCCTYTILMEYLIGEGKCKEALDIFSKM------QDAGVYPDKAA 283
            A   + +M +  + P+  T   L+  ++     ++A  I +        Q A ++ +   
Sbjct: 664  AMCFFHQM-KKLVYPDFVTLCTLLPGVVKASLIEDAYKIITNFLYNCADQPANLFWEDLI 723

Query: 284  CNILIQK----------------CCKSGERLVMTQILEFMKENRFVLRYPVFVEAHETL- 343
             +IL +                  C+ G+ +++  I    K N       +F +  + L 
Sbjct: 724  GSILAEAGIDNAVSFSERLVANGICRDGDSILVPIIRYSCKHNNVSGARTLFEKFTKDLG 783

Query: 344  -KSCSVSYALLKQVNPHMEIESISKGEVVDVSTGSNTVPPNVD--NELLAMLLKDNKLTA 403
             +    +Y LL  +   +E + I   + V +   S    P+V   N LL    K  K+  
Sbjct: 784  VQPKLPTYNLL--IGGLLEADMIEIAQDVFLQVKSTGCIPDVATYNFLLDAYGKSGKIDE 843

Query: 404  VDHMLIGIVDKNIQLDSSIIYSIIEVNCKSNRPNSAL-LAFDYCLKNSVNIKRKLYLDLI 463
            +  +   +     + ++     +I    K+   + AL L +D       +     Y  LI
Sbjct: 844  LFELYKEMSTHECEANTITHNIVISGLVKAGNVDDALDLYYDLMSDRDFSPTACTYGPLI 903

Query: 464  GILIRSSIYPKLLEIVQEMYTQGHCLGLYHATLILYSLGKAGKPQYARKVFNMLPEE--L 523
              L +S    +  ++ + M   G         +++   GKAG+   A  +F  + +E   
Sbjct: 904  DGLSKSGRLYEAKQLFEGMLDYGCRPNCAIYNILINGFGKAGEADAACALFKRMVKEGVR 963

Query: 524  KCTATYTALVDGYFSAGSSGKGLKIFETMRKKGFTPSLGTYNVLLNGLAKNGRGVELNIY 561
                TY+ LVD     G   +GL  F+ +++ G  P +  YN+++NGL K+ R  E  + 
Sbjct: 964  PDLKTYSVLVDCLCMVGRVDEGLHYFKELKESGLNPDVVCYNLIINGLGKSHRLEEALVL 1023

HSP 5 Score: 81.3 bits (199), Expect = 4.0e-14
Identity = 104/424 (24.53%), Postives = 174/424 (41.04%), Query Frame = 1

Query: 136 YTYTTMLDIFGEAGRISSMNYVFQQMKEKGIKIDAVTYTSLMHWRSNSGDVDGAIKVWKE 195
           +TY T++       R+     +F  M+  G+K  A TY   + +   SGD   A++ +++
Sbjct: 399 HTYNTLICGLLRVHRLDDALELFGNMESLGVKPTAYTYIVFIDYYGKSGDSVSALETFEK 458

Query: 196 MKANGCHPTVVSYTAYIKILLDNGQINEATATYKKMLQSGLSPNCCTYTILMEYLIGEGK 255
           MK  G  P +V+  A +  L   G+  EA   +  +   GL P+  TY ++M+     G+
Sbjct: 459 MKTKGIAPNIVACNASLYSLAKAGRDREAKQIFYGLKDIGLVPDSVTYNMMMKCYSKVGE 518

Query: 256 CKEALDIFSKMQDAGVYPDKAACNILIQKCCKSGERLVMTQILEFMKENRFVLRYPVFVE 315
             EA+ + S+M + G  PD    N LI    K+       ++   MKE +          
Sbjct: 519 IDEAIKLLSEMMENGCEPDVIVVNSLINTLYKADRVDEAWKMFMRMKEMK---------- 578

Query: 316 AHETLKSCSVSY-ALLKQVNPHMEIESISKGEVVDVSTG--SNTVPPN--VDNELLAMLL 375
               LK   V+Y  LL  +  + +I+     E +++  G      PPN    N L   L 
Sbjct: 579 ----LKPTVVTYNTLLAGLGKNGKIQ-----EAIELFEGMVQKGCPPNTITFNTLFDCLC 638

Query: 376 KDNKLTAVDHMLIGIVDKNIQLDSSIIYSIIEVNCKSNRPNSALLAFDYCLKNSVNIKRK 435
           K++++T    ML  ++D     D     +II    K+ +   A+  F         +K+ 
Sbjct: 639 KNDEVTLALKMLFKMMDMGCVPDVFTYNTIIFGLVKNGQVKEAMCFFH-------QMKKL 698

Query: 436 LYLDLIGI--LIRSSIYPKLLEIVQEMYTQ------GHCLGLYHATLILYSLGKAGKPQY 495
           +Y D + +  L+   +   L+E   ++ T            L+   LI   L +AG    
Sbjct: 699 VYPDFVTLCTLLPGVVKASLIEDAYKIITNFLYNCADQPANLFWEDLIGSILAEAGIDN- 758

Query: 496 ARKVFNMLPEELKCTATYTALVD--GYFSAGSSGKGLK-IFETMRKK-GFTPSLGTYNVL 543
           A      L     C    + LV    Y    ++  G + +FE   K  G  P L TYN+L
Sbjct: 759 AVSFSERLVANGICRDGDSILVPIIRYSCKHNNVSGARTLFEKFTKDLGVQPKLPTYNLL 795

BLAST of Cucsa.250610 vs. Swiss-Prot
Match: RF1_ORYSI (Protein Rf1, mitochondrial OS=Oryza sativa subsp. indica GN=Rf1 PE=2 SV=1)

HSP 1 Score: 118.6 bits (296), Expect = 2.3e-25
Identity = 110/424 (25.94%), Postives = 190/424 (44.81%), Query Frame = 1

Query: 134 DQYTYTTMLDIFGEAGRISSMNYVFQQMKEKGIKIDAVTYTSLMHWRSNSGDVDGAIKVW 193
           D  +YTT+++ F + G        + +M ++GI  D VTY S++     +  +D A++V 
Sbjct: 195 DVVSYTTVINGFFKEGDSDKAYSTYHEMLDRGILPDVVTYNSIIAALCKAQAMDKAMEVL 254

Query: 194 KEMKANGCHPTVVSYTAYIKILLDNGQINEATATYKKMLQSGLSPNCCTYTILMEYLIGE 253
             M  NG  P  ++Y + +     +GQ  EA    KKM   G+ P+  TY++LM+YL   
Sbjct: 255 NTMVKNGVMPDCMTYNSILHGYCSSGQPKEAIGFLKKMRSDGVEPDVVTYSLLMDYLCKN 314

Query: 254 GKCKEALDIFSKMQDAGVYPDKAACNILIQKCCKSGERLVMTQILEFMKENRFVLRYPVF 313
           G+C EA  IF  M   G+ P+      L+Q     G  + M  +L+ M  N     + VF
Sbjct: 315 GRCMEARKIFDSMTKRGLKPEITTYGTLLQGYATKGALVEMHGLLDLMVRNGIHPDHYVF 374

Query: 314 VEAHETLKSCSVSYALLKQVNPHMEIESISKGEVVDVSTGSNTVPPN--VDNELLAMLLK 373
                ++  C  +YA   +V+  M + S  + +      G N   PN      ++ +L K
Sbjct: 375 -----SILIC--AYAKQGKVDQAMLVFSKMRQQ------GLN---PNAVTYGAVIGILCK 434

Query: 374 DNKLTAVDHMLIGIVDKNIQLDSSIIYSIIEVNCKSNRPNSALLAFDYCLKNSVNIKRKL 433
             ++         ++D+ +   + +  S+I   C  N+   A       L   + +    
Sbjct: 435 SGRVEDAMLYFEQMIDEGLSPGNIVYNSLIHGLCTCNKWERAEELILEMLDRGICLNTIF 494

Query: 434 YLDLI------GILIRSSIYPKLLEIVQEMYTQGHCLGLYHATLILYSLGKAGKPQYARK 493
           +  +I      G +I S    KL E++  +  + + +  Y+  +  Y L  AGK   A K
Sbjct: 495 FNSIIDSHCKEGRVIESE---KLFELMVRIGVKPNVI-TYNTLINGYCL--AGKMDEAMK 554

Query: 494 VFN-MLPEELK-CTATYTALVDGYFSAGSSGKGLKIFETMRKKGFTPSLGTYNVLLNGLA 548
           + + M+   LK  T TY+ L++GY         L +F+ M   G +P + TYN++L GL 
Sbjct: 555 LLSGMVSVGLKPNTVTYSTLINGYCKISRMEDALVLFKEMESSGVSPDIITYNIILQGLF 596

HSP 2 Score: 93.2 bits (230), Expect = 1.0e-17
Identity = 78/338 (23.08%), Postives = 147/338 (43.49%), Query Frame = 1

Query: 127 TLQVFKHDQYTYTTMLDIFGEAGRISSMNYVFQQMKEKGIKIDAVTYTSLMHWRSNSGDV 186
           T +  K +  TY T+L  +   G +  M+ +   M   GI  D   ++ L+   +  G V
Sbjct: 328 TKRGLKPEITTYGTLLQGYATKGALVEMHGLLDLMVRNGIHPDHYVFSILICAYAKQGKV 387

Query: 187 DGAIKVWKEMKANGCHPTVVSYTAYIKILLDNGQINEATATYKKMLQSGLSPNCCTYTIL 246
           D A+ V+ +M+  G +P  V+Y A I IL  +G++ +A   +++M+  GLSP    Y  L
Sbjct: 388 DQAMLVFSKMRQQGLNPNAVTYGAVIGILCKSGRVEDAMLYFEQMIDEGLSPGNIVYNSL 447

Query: 247 MEYLIGEGKCKEALDIFSKMQDAGVYPDKAACNILIQKCCKSGERLVMTQILEFMKE--- 306
           +  L    K + A ++  +M D G+  +    N +I   CK G  +   ++ E M     
Sbjct: 448 IHGLCTCNKWERAEELILEMLDRGICLNTIFFNSIIDSHCKEGRVIESEKLFELMVRIGV 507

Query: 307 -------NRFVLRYPVFVEAHETLKSCS-----------VSYALLKQVNPHMEIESISKG 366
                  N  +  Y +  +  E +K  S           V+Y+ L  +N + +I  +   
Sbjct: 508 KPNVITYNTLINGYCLAGKMDEAMKLLSGMVSVGLKPNTVTYSTL--INGYCKISRMEDA 567

Query: 367 EVVDVSTGSNTVPPNV--DNELLAMLLKDNKLTAVDHMLIGIVDKNIQLDSSIIYSIIEV 426
            V+     S+ V P++   N +L  L +  +  A   + + I +   Q++ S    I+  
Sbjct: 568 LVLFKEMESSGVSPDIITYNIILQGLFQTRRTAAAKELYVRITESGTQIELSTYNIILHG 627

Query: 427 NCKSNRPNSALLAFDYCLKNSVNIKRKLYLDLIGILIR 442
            CK+   + AL  F       + ++ + +  +I  L++
Sbjct: 628 LCKNKLTDDALQMFQNLCLMDLKLEARTFNIMIDALLK 663

HSP 3 Score: 93.2 bits (230), Expect = 1.0e-17
Identity = 96/424 (22.64%), Postives = 175/424 (41.27%), Query Frame = 1

Query: 131 FKHDQYTYTTMLDIFGEAGRIS-SMNYVFQQMKEKGIKIDAVTYTSLMHWRSNSGDVDGA 190
           F+ D   +T +L       R S +M+ V ++M E G   +  +Y  L+    +      A
Sbjct: 118 FRVDAIAFTPLLKGLCADKRTSDAMDIVLRRMTELGCIPNVFSYNILLKGLCDENRSQEA 177

Query: 191 IKVWKEM---KANGCHPTVVSYTAYIKILLDNGQINEATATYKKMLQSGLSPNCCTYTIL 250
           +++   M   +  G  P VVSYT  I      G  ++A +TY +ML  G+ P+  TY  +
Sbjct: 178 LELLHMMADDRGGGSPPDVVSYTTVINGFFKEGDSDKAYSTYHEMLDRGILPDVVTYNSI 237

Query: 251 MEYLIGEGKCKEALDIFSKMQDAGVYPDKAACNILIQKCCKSGERLVMTQILEFMKENRF 310
           +  L       +A+++ + M   GV PD    N ++   C SG+     + + F+K+ R 
Sbjct: 238 IAALCKAQAMDKAMEVLNTMVKNGVMPDCMTYNSILHGYCSSGQ---PKEAIGFLKKMR- 297

Query: 311 VLRYPVFVEAHETLKSCSVSYALLKQVNPHMEIESISKGEVVDVSTGSNTVPP-NVDNEL 370
                      + ++   V+Y+LL           +   ++ D  T     P       L
Sbjct: 298 ----------SDGVEPDVVTYSLLMDY-LCKNGRCMEARKIFDSMTKRGLKPEITTYGTL 357

Query: 371 LAMLLKDNKLTAVDHMLIGIVDKNIQLDSSIIYSIIEVNCKSNRPNSALLAFDYCLKNSV 430
           L        L  +  +L  +V   I  D  +   +I    K  + + A+L F    +  +
Sbjct: 358 LQGYATKGALVEMHGLLDLMVRNGIHPDHYVFSILICAYAKQGKVDQAMLVFSKMRQQGL 417

Query: 431 NIKRKLYLDLIGILIRSSIYPKLLEIVQEMYTQGHCLGLYHATLILYSLGKAGKPQYARK 490
           N     Y  +IGIL +S      +   ++M  +G   G      +++ L    K + A +
Sbjct: 418 NPNAVTYGAVIGILCKSGRVEDAMLYFEQMIDEGLSPGNIVYNSLIHGLCTCNKWERAEE 477

Query: 491 VFNMLPEELKC--TATYTALVDGYFSAGSSGKGLKIFETMRKKGFTPSLGTYNVLLNGLA 548
           +   + +   C  T  + +++D +   G   +  K+FE M + G  P++ TYN L+NG  
Sbjct: 478 LILEMLDRGICLNTIFFNSIIDSHCKEGRVIESEKLFELMVRIGVKPNVITYNTLINGYC 526

HSP 4 Score: 89.4 bits (220), Expect = 1.5e-16
Identity = 93/442 (21.04%), Postives = 180/442 (40.72%), Query Frame = 1

Query: 134 DQYTYTTMLDIFGEAGRISSMNYVFQQMKEKGIKIDAVTYTSLMHWRSNSGDVDGAIKVW 193
           D  TY ++L  +  +G+        ++M+  G++ D VTY+ LM +   +G    A K++
Sbjct: 265 DCMTYNSILHGYCSSGQPKEAIGFLKKMRSDGVEPDVVTYSLLMDYLCKNGRCMEARKIF 324

Query: 194 KEMKANGCHPTVVSYTAYIKILLDNGQINEATATYKKMLQSGLSPNCCTYTILMEYLIGE 253
             M   G  P + +Y   ++     G + E       M+++G+ P+   ++IL+     +
Sbjct: 325 DSMTKRGLKPEITTYGTLLQGYATKGALVEMHGLLDLMVRNGIHPDHYVFSILICAYAKQ 384

Query: 254 GKCKEALDIFSKMQDAGVYPDKAACNILIQKCCKSGERLVMTQILEFMKENRFVLRYPVF 313
           GK  +A+ +FSKM+  G+ P+      +I   CKSG         E M +        V+
Sbjct: 385 GKVDQAMLVFSKMRQQGLNPNAVTYGAVIGILCKSGRVEDAMLYFEQMIDEGLSPGNIVY 444

Query: 314 VEAHETLKSCS----VSYALLKQVNPHMEIESISKGEVVDVSTGSNT------------- 373
                 L +C+        +L+ ++  + + +I    ++D                    
Sbjct: 445 NSLIHGLCTCNKWERAEELILEMLDRGICLNTIFFNSIIDSHCKEGRVIESEKLFELMVR 504

Query: 374 --VPPNV--DNELLAMLLKDNKLTAVDHMLIGIVDKNIQLDSSIIYSIIEVNCKSNRPNS 433
             V PNV   N L+       K+     +L G+V   ++ ++    ++I   CK +R   
Sbjct: 505 IGVKPNVITYNTLINGYCLAGKMDEAMKLLSGMVSVGLKPNTVTYSTLINGYCKISRMED 564

Query: 434 ALLAFDYCLKNSVNIKRKLYLDLIGILIRSSIYPKLLEIVQEMYTQGHCLGLYHATLILY 493
           AL+ F     + V+     Y  ++  L ++       E+   +   G  + L    +IL+
Sbjct: 565 ALVLFKEMESSGVSPDIITYNIILQGLFQTRRTAAAKELYVRITESGTQIELSTYNIILH 624

Query: 494 SLGKAGKPQYARKVF-NMLPEELKCTA-TYTALVDGYFSAGSSGKGLKIFETMRKKGFTP 553
            L K      A ++F N+   +LK  A T+  ++D     G + +   +F      G  P
Sbjct: 625 GLCKNKLTDDALQMFQNLCLMDLKLEARTFNIMIDALLKVGRNDEAKDLFVAFSSNGLVP 684

BLAST of Cucsa.250610 vs. TrEMBL
Match: A0A0A0LJM3_CUCSA (Uncharacterized protein OS=Cucumis sativus GN=Csa_2G295410 PE=4 SV=1)

HSP 1 Score: 1181.0 bits (3054), Expect = 0.0e+00
Identity = 586/588 (99.66%), Postives = 587/588 (99.83%), Query Frame = 1

Query: 1   MHFCNIFSLLLSNYVVSSAIRKRIYQNISSKCLHSLHQYKRDKPISRFSRQSRKGTKVAK 60
           MHFCNIFSLLLSNYVVSSAIRKRIYQNISSKCLHSLHQYKRDKPISRFSRQSRKGTKVAK
Sbjct: 1   MHFCNIFSLLLSNYVVSSAIRKRIYQNISSKCLHSLHQYKRDKPISRFSRQSRKGTKVAK 60

Query: 61  KEEVIPRLYTRDTVRNICNILRNCSWASAQKHLEMLPIRWDSYLINQVLKTHPPLEKTWL 120
           KEEVIPRLYTRDTVRNICNILRNCSWASAQKHLEMLPIRWDSYLINQVLKTHPPLEKTWL
Sbjct: 61  KEEVIPRLYTRDTVRNICNILRNCSWASAQKHLEMLPIRWDSYLINQVLKTHPPLEKTWL 120

Query: 121 FFNWASTLQVFKHDQYTYTTMLDIFGEAGRISSMNYVFQQMKEKGIKIDAVTYTSLMHWR 180
           FFNWASTLQVFKHDQYTYTTMLDIFGEAGRISSMNYVFQQMKEKGIKIDAVTYTSLMHWR
Sbjct: 121 FFNWASTLQVFKHDQYTYTTMLDIFGEAGRISSMNYVFQQMKEKGIKIDAVTYTSLMHWR 180

Query: 181 SNSGDVDGAIKVWKEMKANGCHPTVVSYTAYIKILLDNGQINEATATYKKMLQSGLSPNC 240
           SNSGDVDGAIK+WKEMKANGCHPTVVSYTAYIKILLDNGQINEATATYKKMLQSGLSPNC
Sbjct: 181 SNSGDVDGAIKLWKEMKANGCHPTVVSYTAYIKILLDNGQINEATATYKKMLQSGLSPNC 240

Query: 241 CTYTILMEYLIGEGKCKEALDIFSKMQDAGVYPDKAACNILIQKCCKSGERLVMTQILEF 300
           CTYTILMEYLIGEGKCKEALDIFSKMQDAGVYPDKAACNILIQKCCKSGERLVMTQILEF
Sbjct: 241 CTYTILMEYLIGEGKCKEALDIFSKMQDAGVYPDKAACNILIQKCCKSGERLVMTQILEF 300

Query: 301 MKENRFVLRYPVFVEAHETLKSCSVSYALLKQVNPHMEIESISKGEVVDVSTGSNTVPPN 360
           MKENRFVLRYPVFVEAHETLKSCSVSYALLKQVNPHMEIESISKGEVVDVSTGSNTVPPN
Sbjct: 301 MKENRFVLRYPVFVEAHETLKSCSVSYALLKQVNPHMEIESISKGEVVDVSTGSNTVPPN 360

Query: 361 VDNELLAMLLKDNKLTAVDHMLIGIVDKNIQLDSSIIYSIIEVNCKSNRPNSALLAFDYC 420
           VDNELLAMLLKDNKLTAVDHMLIGIVDKNIQLDSSIIYSIIEVNCKSNRPNSALLAFDYC
Sbjct: 361 VDNELLAMLLKDNKLTAVDHMLIGIVDKNIQLDSSIIYSIIEVNCKSNRPNSALLAFDYC 420

Query: 421 LKNSVNIKRKLYLDLIGILIRSSIYPKLLEIVQEMYTQGHCLGLYHATLILYSLGKAGKP 480
           LKNSVNIKRKLYLDLIGILIRSSIYPKLLEIVQEMYTQGHCLGLYHATLIL SLGKAGKP
Sbjct: 421 LKNSVNIKRKLYLDLIGILIRSSIYPKLLEIVQEMYTQGHCLGLYHATLILCSLGKAGKP 480

Query: 481 QYARKVFNMLPEELKCTATYTALVDGYFSAGSSGKGLKIFETMRKKGFTPSLGTYNVLLN 540
           QYARKVFNMLPEELKCTATYTALVDGYFSAGSSGKGLKIFETMRKKGFTPSLGTYNVLLN
Sbjct: 481 QYARKVFNMLPEELKCTATYTALVDGYFSAGSSGKGLKIFETMRKKGFTPSLGTYNVLLN 540

Query: 541 GLAKNGRGVELNIYRREKKSFEISHHSRLNTILDDERICDLLFGELVS 589
           GLAKNGRGVELNIYRREKKSFEISHHSRLNTILDDERICDLLFGELVS
Sbjct: 541 GLAKNGRGVELNIYRREKKSFEISHHSRLNTILDDERICDLLFGELVS 588

BLAST of Cucsa.250610 vs. TrEMBL
Match: A0A0D2QLU1_GOSRA (Uncharacterized protein OS=Gossypium raimondii GN=B456_007G051500 PE=4 SV=1)

HSP 1 Score: 622.5 bits (1604), Expect = 5.4e-175
Identity = 316/576 (54.86%), Postives = 411/576 (71.35%), Query Frame = 1

Query: 9   LLLSNYVVSSAIRKRIYQNISSKCLHSLHQYKRDKPISRFSRQSRKGTKVAKKE-EVIPR 68
           +L  NY+   A + R + N   K LHSLHQ KR  P  R   +  K  K   K  +V P+
Sbjct: 1   MLRINYLHGLAAKFRNW-NRPLKFLHSLHQNKRRTP--RTKHKFTKALKTPMKPFDVEPK 60

Query: 69  LYTRDTVRNICNILRNCSWASAQKHLEMLPIRWDSYLINQVLKTHPPLEKTWLFFNWAST 128
           +Y RDT+ NI  IL+  +W SA+  L+ LPIRWDS+ +NQVLKTHPP+EK WLFFNW   
Sbjct: 61  VYMRDTISNIYKILKYSTWESAETQLKQLPIRWDSFTVNQVLKTHPPMEKAWLFFNWVGK 120

Query: 129 LQVFKHDQYTYTTMLDIFGEAGRISSMNYVFQQMKEKGIKIDAVTYTSLMHWRSNSGDVD 188
           ++ FKHDQ+TYTTMLDIFGEAGR+SSM Y+FQQM+EKG+KIDAVTYTS++HW S SGDVD
Sbjct: 121 VKSFKHDQFTYTTMLDIFGEAGRVSSMKYLFQQMQEKGLKIDAVTYTSILHWLSKSGDVD 180

Query: 189 GAIKVWKEMKANGCHPTVVSYTAYIKILLDNGQINEATATYKKMLQSGLSPNCCTYTILM 248
           GA++ W+EM+  GC PTVVSYTAY+K+L DN ++ E TA YK+MLQSG+SPNC TYT+LM
Sbjct: 181 GAVETWEEMRGKGCFPTVVSYTAYMKVLFDNQRVKEGTAVYKEMLQSGISPNCHTYTVLM 240

Query: 249 EYLIGEGKCKEALDIFSKMQDAGVYPDKAACNILIQKCCKSGERLVMTQILEFMKENRFV 308
           EYL G GK +EAL+IFSKMQ+A V PDKAACNIL++KCCK+GE   + QIL++MKEN  V
Sbjct: 241 EYLFGAGKYEEALEIFSKMQEARVKPDKAACNILVEKCCKAGETWPIIQILQYMKENHLV 300

Query: 309 LRYPVFVEAHETLKSCSVSYALLKQVNPHMEIESISKGEVVDVSTGSNTVPPNVDNELLA 368
           LRYP+F+ A ET K+     ALL+QV+PH+ +E I    VV+        P ++D  L+ 
Sbjct: 301 LRYPIFLLALETFKAAGERDALLRQVHPHISVECIDNERVVEYKGNDFEDPSSLDRGLVW 360

Query: 369 MLLKDNKLTAVDHMLIGIVDKNIQLDSSIIYSIIEVNCKSNRPNSALLAFDYCLKNSVNI 428
           +LLK   L AVD +L  ++DKNI+LDS ++ +I+ VNC   R + ALL F Y +K  + +
Sbjct: 361 VLLKKQNLQAVDSLLTELMDKNIKLDSELMSAIVHVNCGHCRVDGALLTFKYSVKTGIKL 420

Query: 429 KRKLYLDLIGILIRSSIYPKLLEIVQEMYTQGHCLGLYHATLILYSLGKAGKPQYARKVF 488
           +R  YL LIG LIRS+ +  ++EIV EM + GH LG+Y A+L++Y LG A +P  A K+F
Sbjct: 421 ERTAYLALIGCLIRSNTFTDIVEIVVEMTSTGHSLGVYLASLLIYRLGCARRPTCAAKIF 480

Query: 489 NMLPEELKCTATYTALVDGYFSAGSSGKGLKIFETMRKKGFTPSLGTYNVLLNGLAKNGR 548
           + LP++ KC ATYTALV  YF+AG++ KGLKI++TMRKKG  PSLGTY VL+ GL K  R
Sbjct: 481 DSLPDDQKCVATYTALVGVYFAAGTADKGLKIYKTMRKKGIYPSLGTYCVLVAGLEKLSR 540

Query: 549 GVELNIYRREKKSFEISHHSRLNTILDDERICDLLF 584
                 YR+EKKS     + R  +I  +E+ICDLLF
Sbjct: 541 VSNAETYRKEKKSLRKDAYFR-ESIPMEEKICDLLF 572

BLAST of Cucsa.250610 vs. TrEMBL
Match: A0A0B0PHY7_GOSAR (Uncharacterized protein OS=Gossypium arboreum GN=F383_31667 PE=4 SV=1)

HSP 1 Score: 614.0 bits (1582), Expect = 1.9e-172
Identity = 301/524 (57.44%), Postives = 390/524 (74.43%), Query Frame = 1

Query: 60  KKEEVIPRLYTRDTVRNICNILRNCSWASAQKHLEMLPIRWDSYLINQVLKTHPPLEKTW 119
           K  +V P++Y RDT+ NIC IL+  +W SA+  L+ LPIRWDS+ +NQVLKTHPP+EK W
Sbjct: 2   KPFDVEPKVYMRDTISNICKILKYSTWESAETQLKQLPIRWDSFTVNQVLKTHPPMEKAW 61

Query: 120 LFFNWASTLQVFKHDQYTYTTMLDIFGEAGRISSMNYVFQQMKEKGIKIDAVTYTSLMHW 179
           LFFNW   ++ FKHDQ+TYTTMLDIFGEAGR+SSM Y+FQQM+EKG+KIDAVTYTS++HW
Sbjct: 62  LFFNWVGKVKSFKHDQFTYTTMLDIFGEAGRVSSMKYLFQQMQEKGLKIDAVTYTSILHW 121

Query: 180 RSNSGDVDGAIKVWKEMKANGCHPTVVSYTAYIKILLDNGQINEATATYKKMLQSGLSPN 239
            S S DVDGA++ W+EM+  GC PTVVSYTAY+K+L DN ++ E TA YK+MLQSG+SPN
Sbjct: 122 LSKSEDVDGAVETWEEMRGKGCFPTVVSYTAYMKVLFDNQRVKEGTAVYKEMLQSGISPN 181

Query: 240 CCTYTILMEYLIGEGKCKEALDIFSKMQDAGVYPDKAACNILIQKCCKSGERLVMTQILE 299
           C TYT+LMEYL G GK +EAL+IFSKMQ+A V PDKAACNIL++KCCK+GE   + QIL+
Sbjct: 182 CHTYTVLMEYLFGAGKYEEALEIFSKMQEARVMPDKAACNILVEKCCKAGETWPIIQILQ 241

Query: 300 FMKENRFVLRYPVFVEAHETLKSCSVSYALLKQVNPHMEIESISKGEVVDVSTGSNTVPP 359
           +MKEN  VLRYP+F+ A ETLK+     ALL+QV+PH+ +E I  G VV+        P 
Sbjct: 242 YMKENHLVLRYPIFLLALETLKAAGERDALLRQVHPHISVECIDNGRVVEYKGNDFEDPS 301

Query: 360 NVDNELLAMLLKDNKLTAVDHMLIGIVDKNIQLDSSIIYSIIEVNCKSNRPNSALLAFDY 419
           ++D  L+ +LLK   L AVD +L  ++DKNI+LDS ++ SI+ VNC   R   ALLAF Y
Sbjct: 302 SLDRGLVWVLLKKLNLQAVDSLLTELMDKNIKLDSELMSSIVHVNCGHCRVGGALLAFKY 361

Query: 420 CLKNSVNIKRKLYLDLIGILIRSSIYPKLLEIVQEMYTQGHCLGLYHATLILYSLGKAGK 479
            +K  + ++R  YL LIG LIRS+ +  ++EIV EM + GH LG+Y A+L++Y LG A +
Sbjct: 362 SVKTGIKLERTGYLALIGCLIRSNRFTDIVEIVVEMTSTGHSLGVYLASLLIYRLGCARR 421

Query: 480 PQYARKVFNMLPEELKCTATYTALVDGYFSAGSSGKGLKIFETMRKKGFTPSLGTYNVLL 539
           P  A K+F+ LP++ KC ATYTALV  YF+AG++ KGLKI++ MRKKG  PSLGTY VL+
Sbjct: 422 PTCAAKIFDSLPDDQKCVATYTALVGVYFAAGTADKGLKIYKAMRKKGIYPSLGTYCVLV 481

Query: 540 NGLAKNGRGVELNIYRREKKSFEISHHSRLNTILDDERICDLLF 584
            GL K GR      YR+EKKS +   + R  +I  +E+ICDLLF
Sbjct: 482 AGLEKLGRVSNAETYRKEKKSLQKDAYFR-ESIPMEEKICDLLF 524

BLAST of Cucsa.250610 vs. TrEMBL
Match: A0A061DFX4_THECC (Tetratricopeptide repeat-like superfamily protein OS=Theobroma cacao GN=TCM_000458 PE=4 SV=1)

HSP 1 Score: 609.0 bits (1569), Expect = 6.1e-171
Identity = 301/553 (54.43%), Postives = 398/553 (71.97%), Query Frame = 1

Query: 31  KCLHSLHQYKRDKPISRFSRQSRKGTKVAKKEEVIPRLYTRDTVRNICNILRNCSWASAQ 90
           KCLHS  Q+K + P      ++ K     K  EV P++Y RDT+ NI  IL+  +W +A+
Sbjct: 22  KCLHSFDQHKPETPTKYKFTKTLKNPM--KPLEVEPKVYMRDTISNIYKILKYSTWDAAE 81

Query: 91  KHLEMLPIRWDSYLINQVLKTHPPLEKTWLFFNWASTLQVFKHDQYTYTTMLDIFGEAGR 150
             L+ L I+WDS+ INQVLKTHPP+EK WLFFNW   ++ FKHDQ+TYTTMLDIFGEAGR
Sbjct: 82  TQLKRLRIKWDSFTINQVLKTHPPMEKAWLFFNWVGKVKGFKHDQFTYTTMLDIFGEAGR 141

Query: 151 ISSMNYVFQQMKEKGIKIDAVTYTSLMHWRSNSGDVDGAIKVWKEMKANGCHPTVVSYTA 210
           +SSM Y+FQQM+EKG+KIDAV YTS++HW S S DVDGA+++W+EM+  GC PTVVSYTA
Sbjct: 142 VSSMKYLFQQMQEKGLKIDAVAYTSVLHWLSKSRDVDGAVEMWEEMRGKGCFPTVVSYTA 201

Query: 211 YIKILLDNGQINEATATYKKMLQSGLSPNCCTYTILMEYLIGEGKCKEALDIFSKMQDAG 270
           Y+K+L DN ++ E T  YK+MLQSG+SPNC TYT+LMEYL   GK +EAL+IF+KMQ+AG
Sbjct: 202 YMKVLFDNKRVKEGTDVYKEMLQSGISPNCHTYTVLMEYLFEAGKSEEALEIFNKMQEAG 261

Query: 271 VYPDKAACNILIQKCCKSGERLVMTQILEFMKENRFVLRYPVFVEAHETLKSCSVSYALL 330
           V PDKAACNIL++KCCK+ E   MTQIL++MKEN  VLRYP+F+EA ET K    S  LL
Sbjct: 262 VKPDKAACNILVEKCCKAAETRAMTQILQYMKENYLVLRYPIFLEALETFKVAGESNVLL 321

Query: 331 KQVNPHMEIESISKGEVVDVSTGSNTVPPNVDNELLAMLLKDNKLTAVDHMLIGIVDKNI 390
           ++V+PH+ +E I      +    ++  P + D  L+  LLK   L A+D +L  ++DKNI
Sbjct: 322 REVHPHISVECIGNETEAEYKGNASEAPLSFDRGLMWALLKKQNLLAIDSLLTELMDKNI 381

Query: 391 QLDSSIIYSIIEVNCKSNRPNSALLAFDYCLKNSVNIKRKLYLDLIGILIRSSIYPKLLE 450
           +LDS +I +II++NC   R + +LLAF Y +K  +N++R  YL LIG LIRS+ +  ++E
Sbjct: 382 RLDSEMISTIIDINCNHCRLDGSLLAFKYSVKAGINLERAAYLTLIGSLIRSNTFTDVVE 441

Query: 451 IVQEMYTQGHCLGLYHATLILYSLGKAGKPQYARKVFNMLPEELKCTATYTALVDGYFSA 510
           IV EM   GH  G+Y  +L++Y LG A +P  A K+FN+LP++ KC ATYTALV  YF+A
Sbjct: 442 IVVEMTRAGHSPGVYLGSLLIYRLGCARRPTCAAKIFNLLPDDQKCVATYTALVGVYFAA 501

Query: 511 GSSGKGLKIFETMRKKGFTPSLGTYNVLLNGLAKNGRGVELNIYRREKKSFEISHHSRLN 570
           G++ KGLKI++TMR KG +PSLGTY VLL GL K GR      YR+EKKS +   + R  
Sbjct: 502 GTADKGLKIYKTMRSKGISPSLGTYCVLLAGLEKLGRVSTAETYRKEKKSLQKDAYFR-E 561

Query: 571 TILDDERICDLLF 584
           +I  +E+ICDLLF
Sbjct: 562 SIPIEEKICDLLF 571

BLAST of Cucsa.250610 vs. TrEMBL
Match: M5W8D9_PRUPE (Uncharacterized protein OS=Prunus persica GN=PRUPE_ppa004294mg PE=4 SV=1)

HSP 1 Score: 606.7 bits (1563), Expect = 3.0e-170
Identity = 305/519 (58.77%), Postives = 387/519 (74.57%), Query Frame = 1

Query: 71  RDTVRNICNILRNCSWASAQKHLEMLPIRWDSYLINQVLKTHPPLEKTWLFFNWASTLQV 130
           R+T+ NI  IL+  +W SAQ  L+ LPIRWDSY ++QVLKTHPP+EK WLFFNW + ++ 
Sbjct: 2   RETIGNIYKILKYSTWDSAQDQLQRLPIRWDSYTVSQVLKTHPPMEKAWLFFNWVAGIRG 61

Query: 131 FKHDQYTYTTMLDIFGEAGRISSMNYVFQQMKEKGIKIDAVTYTSLMHWRSNSGDVDGAI 190
           FKHD +TYTTMLDIFGEAGR+SSM +VF+QM+EK I+IDA TYTSLMHW S++GDVDGA+
Sbjct: 62  FKHDHFTYTTMLDIFGEAGRVSSMTHVFKQMQEKDIRIDAFTYTSLMHWLSSAGDVDGAL 121

Query: 191 KVWKEMKANGCHPTVVSYTAYIKILLDNGQINEATATYKKMLQSGLSPNCCTYTILMEYL 250
           KVW+EM+A GC PTVVSYTAY+K+L ++ ++ EA   YK+MLQSG SP C TYT+LMEYL
Sbjct: 122 KVWEEMRAQGCVPTVVSYTAYMKVLFNDNRVKEAADVYKEMLQSGCSPTCHTYTVLMEYL 181

Query: 251 IGEGKCKEALDIFSKMQDAGVYPDKAACNILIQKCCKSGERLVMTQILEFMKENRFVLRY 310
           IG GKCKEAL+IF KMQDAGV PDKAACNILI+  CK GE   M Q+L FMKE+R  LRY
Sbjct: 182 IGSGKCKEALEIFGKMQDAGVQPDKAACNILIENLCKVGETWTMNQVLWFMKEHRLALRY 241

Query: 311 PVFVEAHETLKSCSVSYALLKQVNPHMEIESISKGEVVDVSTGSNTVPPNVDNELLAMLL 370
           PVF+EA  TLK   VS +LL+QV+PH  IES    E  ++   +   P  +D  L+ +LL
Sbjct: 242 PVFLEAIRTLKIAGVSDSLLRQVHPHFSIES-GNQETGELRATAADAPSTMDEWLVLILL 301

Query: 371 KDNKLTAVDHMLIGIVDKNIQLDSSIIYSIIEVNCKSNRPNSALLAFDYCLKNSVNIKRK 430
           K   L A+DH+L G   KNI+L+S+II +IIEVNC   RP+ ALLAF+Y +K  + ++R 
Sbjct: 302 KKENLVAIDHLLAGNEGKNIKLNSAIISTIIEVNCGLCRPDGALLAFEYSVKMGIIVERN 361

Query: 431 LYLDLIGILIRSSIYPKLLEIVQEMYTQGHCLGLYHATLILYSLGKAGKPQYARKVFNML 490
            YL LIG LIR S +PK++EIV EM   G+  G Y + L++Y LG+A +   A KVFN+L
Sbjct: 362 AYLSLIGALIRLSSFPKVVEIVVEMIRAGYSPGTYLSALLIYRLGRARRTNCAAKVFNLL 421

Query: 491 PEELKCTATYTALVDGYFSAGSSGKGLKIFETMRKKGFTPSLGTYNVLLNGLAKNGRGVE 550
           P++ KCT TYTAL+  YFSAGS+ +GLKIF+TMR +GF P LGTYNVLL GL K GR  E
Sbjct: 422 PDDHKCTTTYTALMGVYFSAGSADRGLKIFDTMRGEGFLPFLGTYNVLLAGLQKLGRVRE 481

Query: 551 LNIYRREKKSFEISHHSRLNTILDDERICDLLF-GELVS 589
             +YR+EKKS +    S+ N +  D++ICD LF G++VS
Sbjct: 482 AEMYRKEKKSLQSDGPSQ-NAVPIDQKICDFLFAGDVVS 518

BLAST of Cucsa.250610 vs. TAIR10
Match: AT2G01390.1 (AT2G01390.1 Tetratricopeptide repeat (TPR)-like superfamily protein)

HSP 1 Score: 551.2 bits (1419), Expect = 7.7e-157
Identity = 288/558 (51.61%), Postives = 383/558 (68.64%), Query Frame = 1

Query: 29  SSKCLHSLHQYKRDKPISRFSRQSRKGTKVAKKEEVIPRLYTRDTVRNICNILRNCSWAS 88
           S K LHSL + K     +   R S+K   V  +    P +YTRD V NI NIL+  +W S
Sbjct: 20  SVKLLHSLPRLKP----TNSKRFSQKPKLVKTQTLPDPSVYTRDIVSNIYNILKYSNWDS 79

Query: 89  AQKHLEMLPIRWDSYLINQVLKTHPPLEKTWLFFNWASTLQVFKHDQYTYTTMLDIFGEA 148
           AQ+ L  L +RWDS++IN+VLK HPP++K WLFFNWA+ ++ FKHD +TYTTMLDIFGEA
Sbjct: 80  AQEQLPHLGVRWDSHIINRVLKAHPPMQKAWLFFNWAAQIKGFKHDHFTYTTMLDIFGEA 139

Query: 149 GRISSMNYVFQQMKEKGIKIDAVTYTSLMHWRSNSGDVDGAIKVWKEMKANGCHPTVVSY 208
           GRI SM  VF  MKEKG+ ID VTYTSL+HW S+SGDVDGA+++W+EM+ NGC PTVVSY
Sbjct: 140 GRIQSMYSVFHLMKEKGVLIDTVTYTSLIHWVSSSGDVDGAMRLWEEMRDNGCEPTVVSY 199

Query: 209 TAYIKILLDNGQINEATATYKKMLQSGLSPNCCTYTILMEYLIGEGKCKEALDIFSKMQD 268
           TAY+K+L  +G++ EAT  YK+ML+S +SPNC TYT+LMEYL+  GKC+EALDIF KMQ+
Sbjct: 200 TAYMKMLFADGRVEEATEVYKEMLRSRVSPNCHTYTVLMEYLVATGKCEEALDIFFKMQE 259

Query: 269 AGVYPDKAACNILIQKCCKSGERLVMTQILEFMKENRFVLRYPVFVEAHETLKSCSVSYA 328
            GV PDKAACNILI K  K GE   MT++L +MKEN  VLRYP+FVEA ETLK+   S  
Sbjct: 260 IGVQPDKAACNILIAKALKFGETSFMTRVLVYMKENGVVLRYPIFVEALETLKAAGESDD 319

Query: 329 LLKQVNPHMEIESISKGEVVDVSTGSNTVPPNVDNE--LLAMLLKDNKLTAVDHMLIGIV 388
           LL++VN H+ +ES+   ++ +  T       N D+   + ++LL    L AVD +L  + 
Sbjct: 320 LLREVNSHISVESLCSSDIDETPTAEVNDTKNSDDSRVISSVLLMKQNLVAVDILLNQMR 379

Query: 389 DKNIQLDSSIIYSIIEVNCKSNRPNSALLAFDYCLKNSVNIKRKLYLDLIGILIRSSIYP 448
           D+NI+LDS ++ +IIE NC   R   A LAFDY L+  +++K+  YL LIG  +RS+  P
Sbjct: 380 DRNIKLDSFVVSAIIETNCDRCRTEGASLAFDYSLEMGIHLKKSAYLALIGNFLRSNELP 439

Query: 449 KLLEIVQEMYTQGHCLGLYHATLILYSLGKAGKPQYARKVFNMLPEELKCTATYTALVDG 508
           K++E+V+EM    H LG Y   ++++ LG   +P+ A  VF++LP++ K  A YTAL+D 
Sbjct: 440 KVIEVVKEMVKAQHSLGCYQGAMLIHRLGFGRRPRLAADVFDLLPDDQKGVAAYTALMDV 499

Query: 509 YFSAGSSGKGLKIFETMRKKGFTPSLGTYNVLLNGLAKNGR-GVELNIYRREKKSFEISH 568
           Y SAGS  K +KI   MR++   PSLGTY+VLL+GL K      E+ + R+EKKS   S 
Sbjct: 500 YISAGSPEKAMKILREMREREIMPSLGTYDVLLSGLEKTSDFQKEVALLRKEKKSLVASA 559

Query: 569 HSRLNTILDDERICDLLF 584
             R N  ++D +ICDLLF
Sbjct: 560 RFRENVHVED-KICDLLF 572

BLAST of Cucsa.250610 vs. TAIR10
Match: AT1G74750.1 (AT1G74750.1 Pentatricopeptide repeat (PPR) superfamily protein)

HSP 1 Score: 123.6 bits (309), Expect = 4.0e-28
Identity = 82/260 (31.54%), Postives = 124/260 (47.69%), Query Frame = 1

Query: 29  SSKCLHSLHQYKRDKPISR---FSRQSRKGTKVAKKEEVIPRLYTRD--TVRNICNILRN 88
           S  C +S+H       IS    F + SR+  KV  +    PR +      V N+ +ILR 
Sbjct: 247 SGGCDYSVHSSDDRTIISSVEGFGKPSREMMKVTPRTAPTPRQHCNPGYVVENVSSILRR 306

Query: 89  CSWA-SAQKHLEMLPIRWDSYLINQVLKTHPPLEKTWLFFNWASTLQVFKHDQYTYTTML 148
             W  +A++ L     R D+Y  NQVLK          FF W      FKHD +TYTTM+
Sbjct: 307 FKWGHAAEEALHNFGFRMDAYQANQVLKQMDNYANALGFFYWLKRQPGFKHDGHTYTTMV 366

Query: 149 DIFGEAGRISSMNYVFQQMKEKGIKIDAVTYTSLMHWRSNSGDVDGAIKVWKEMKANGCH 208
              G A +   +N +  +M   G K + VTY  L+H    +  +  A+ V+ +M+  GC 
Sbjct: 367 GNLGRAKQFGEINKLLDEMVRDGCKPNTVTYNRLIHSYGRANYLKEAMNVFNQMQEAGCE 426

Query: 209 PTVVSYTAYIKILLDNGQINEATATYKKMLQSGLSPNCCTYTILMEYLIGEGKCKEALDI 268
           P  V+Y   I I    G ++ A   Y++M ++GLSP+  TY++++  L   G    A  +
Sbjct: 427 PDRVTYCTLIDIHAKAGFLDIAMDMYQRMQEAGLSPDTFTYSVIINCLGKAGHLPAAHRL 486

Query: 269 FSKMQDAGVYPDKAACNILI 283
           F +M   G  P+    NI+I
Sbjct: 487 FCEMVGQGCTPNLVTFNIMI 506

HSP 2 Score: 84.0 bits (206), Expect = 3.5e-16
Identity = 46/156 (29.49%), Postives = 82/156 (52.56%), Query Frame = 1

Query: 134 DQYTYTTMLDIFGEAGRISSMNYVFQQMKEKGIKIDAVTYTSLMHWRSNSGDVDGAIKVW 193
           D+ TY T++DI  +AG +     ++Q+M+E G+  D  TY+ +++    +G +  A +++
Sbjct: 428 DRVTYCTLIDIHAKAGFLDIAMDMYQRMQEAGLSPDTFTYSVIINCLGKAGHLPAAHRLF 487

Query: 194 KEMKANGCHPTVVSYTAYIKILLDNGQINEATATYKKMLQSGLSPNCCTYTILMEYLIGE 253
            EM   GC P +V++   I +         A   Y+ M  +G  P+  TY+I+ME L   
Sbjct: 488 CEMVGQGCTPNLVTFNIMIALHAKARNYETALKLYRDMQNAGFQPDKVTYSIVMEVLGHC 547

Query: 254 GKCKEALDIFSKMQDAGVYPDKAACNILIQKCCKSG 290
           G  +EA  +F++MQ     PD+    +L+    K+G
Sbjct: 548 GFLEEAEGVFAEMQRKNWVPDEPVYGLLVDLWGKAG 583

HSP 3 Score: 59.7 bits (143), Expect = 7.0e-09
Identity = 36/149 (24.16%), Postives = 69/149 (46.31%), Query Frame = 1

Query: 137 TYTTMLDIFGEAGRISSMNYVFQQMKEKGIKIDAVTYTSLMHWRSNSGDVDGAIKVWKEM 196
           T+  M+ +  +A    +   +++ M+  G + D VTY+ +M    + G ++ A  V+ EM
Sbjct: 501 TFNIMIALHAKARNYETALKLYRDMQNAGFQPDKVTYSIVMEVLGHCGFLEEAEGVFAEM 560

Query: 197 KANGCHPTVVSYTAYIKILLDNGQINEATATYKKMLQSGLSPNCCTYTILMEYLIGEGKC 256
           +     P    Y   + +    G +++A   Y+ MLQ+GL PN  T   L+   +   + 
Sbjct: 561 QRKNWVPDEPVYGLLVDLWGKAGNVDKAWQWYQAMLQAGLRPNVPTCNSLLSTFLRVHRM 620

Query: 257 KEALDIFSKMQDAGVYPDKAACNILIQKC 286
            EA ++   M   G++P      +L+  C
Sbjct: 621 SEAYNLLQSMLALGLHPSLQTYTLLLSCC 649

HSP 4 Score: 45.4 bits (106), Expect = 1.4e-04
Identity = 28/97 (28.87%), Postives = 46/97 (47.42%), Query Frame = 1

Query: 207 SYTAYIKILLDNGQINEATATYKKMLQSGLSPNCCTYTILMEYLIGEGKCKEALDIFSKM 266
           +YT  +  L    Q  E      +M++ G  PN  TY  L+         KEA+++F++M
Sbjct: 361 TYTTMVGNLGRAKQFGEINKLLDEMVRDGCKPNTVTYNRLIHSYGRANYLKEAMNVFNQM 420

Query: 267 QDAGVYPDKAACNILIQKCCKSGERLVMTQILEFMKE 304
           Q+AG  PD+     LI    K+G   +   + + M+E
Sbjct: 421 QEAGCEPDRVTYCTLIDIHAKAGFLDIAMDMYQRMQE 457

BLAST of Cucsa.250610 vs. TAIR10
Match: AT5G65560.1 (AT5G65560.1 Pentatricopeptide repeat (PPR) superfamily protein)

HSP 1 Score: 120.6 bits (301), Expect = 3.4e-27
Identity = 101/424 (23.82%), Postives = 192/424 (45.28%), Query Frame = 1

Query: 134 DQYTYTTMLDIFGEAGRISSMNYVFQQMKEKGIKIDAVTYTSLMHWRSNSGDVDGAIKVW 193
           DQ+TYT+M+D   ++ R+     +F  +++KG+  + V YT+L+     +G VD A  + 
Sbjct: 496 DQWTYTSMIDSLCKSKRVEEACDLFDSLEQKGVNPNVVMYTALIDGYCKAGKVDEAHLML 555

Query: 194 KEMKANGCHPTVVSYTAYIKILLDNGQINEATATYKKMLQSGLSPNCCTYTILMEYLIGE 253
           ++M +  C P  +++ A I  L  +G++ EAT   +KM++ GL P   T TIL+  L+ +
Sbjct: 556 EKMLSKNCLPNSLTFNALIHGLCADGKLKEATLLEEKMVKIGLQPTVSTDTILIHRLLKD 615

Query: 254 GKCKEALDIFSKMQDAGVYPDKAACNILIQKCCKSGERLVMTQILEFMKENRF---VLRY 313
           G    A   F +M  +G  PD       IQ  C+ G  L    ++  M+EN     +  Y
Sbjct: 616 GDFDHAYSRFQQMLSSGTKPDAHTYTTFIQTYCREGRLLDAEDMMAKMRENGVSPDLFTY 675

Query: 314 PVFVEAHETLKSCSVSYALLKQV-------NPHMEIESISKGEVVDVSTGSNTVPPNVDN 373
              ++ +  L   + ++ +LK++       + H  +  I    ++++  G        + 
Sbjct: 676 SSLIKGYGDLGQTNFAFDVLKRMRDTGCEPSQHTFLSLIK--HLLEMKYGKQ---KGSEP 735

Query: 374 ELLAMLLKDNKLTAVDHMLIGIVDKNIQLDSSIIYSIIEVNCKSNRPNSALLAFDYCLKN 433
           EL AM       T V+ +L  +V+ ++  ++     +I   C+      A   FD+  +N
Sbjct: 736 ELCAMSNMMEFDTVVE-LLEKMVEHSVTPNAKSYEKLILGICEVGNLRVAEKVFDHMQRN 795

Query: 434 -SVNIKRKLYLDLIGILIRSSIYPKLLEIVQEMYTQGHCLGLYHATLILYSLGKAGKPQY 493
             ++    ++  L+    +   + +  ++V +M   GH   L    +++  L K G+ + 
Sbjct: 796 EGISPSELVFNALLSCCCKLKKHNEAAKVVDDMICVGHLPQLESCKVLICGLYKKGEKER 855

Query: 494 ARKVFNMLPEELKC-----TATYTALVDGYFSAGSSGKGLKIFETMRKKGFTPSLGTYNV 542
              VF  L   L+C        +  ++DG    G      ++F  M K G   S  TY++
Sbjct: 856 GTSVFQNL---LQCGYYEDELAWKIIIDGVGKQGLVEAFYELFNVMEKNGCKFSSQTYSL 910

HSP 2 Score: 114.0 bits (284), Expect = 3.1e-25
Identity = 107/423 (25.30%), Postives = 180/423 (42.55%), Query Frame = 1

Query: 136 YTYTTMLDIFGEAGRISSMNYVFQQMKEKGIKIDAVTYTSLMHWRSNSGDVDGAIKVWKE 195
           YTY  M++ + + G +   N    ++ E G+  D  TYTSL+       D+D A KV+ E
Sbjct: 219 YTYNKMVNGYCKLGNVEEANQYVSKIVEAGLDPDFFTYTSLIMGYCQRKDLDSAFKVFNE 278

Query: 196 MKANGCHPTVVSYTAYIKILLDNGQINEATATYKKMLQSGLSPNCCTYTILMEYLIGEGK 255
           M   GC    V+YT  I  L    +I+EA   + KM      P   TYT+L++ L G  +
Sbjct: 279 MPLKGCRRNEVAYTHLIHGLCVARRIDEAMDLFVKMKDDECFPTVRTYTVLIKSLCGSER 338

Query: 256 CKEALDIFSKMQDAGVYPDKAACNILIQKCCKSGERLVMTQILEFMKENRFVLRYPVFVE 315
             EAL++  +M++ G+ P+     +LI   C   +     ++L  M E            
Sbjct: 339 KSEALNLVKEMEETGIKPNIHTYTVLIDSLCSQCKFEKARELLGQMLE------------ 398

Query: 316 AHETLKSCSVSYALLKQVNPHMEIESISKGEVVDVSTGSNTVPPNVD--NELLAMLLKDN 375
             + L    ++Y  L  +N + +   I     V     S  + PN    NEL+    K N
Sbjct: 399 --KGLMPNVITYNAL--INGYCKRGMIEDAVDVVELMESRKLSPNTRTYNELIKGYCKSN 458

Query: 376 KLTAVDHMLIGIVDKNIQ---LDSSIIY-SIIEVNCKSNRPNSALLAFDYCLKNSVNIKR 435
                 H  +G+++K ++   L   + Y S+I+  C+S   +SA           +   +
Sbjct: 459 V-----HKAMGVLNKMLERKVLPDVVTYNSLIDGQCRSGNFDSAYRLLSLMNDRGLVPDQ 518

Query: 436 KLYLDLIGILIRSSIYPKLLEIVQEMYTQGHCLGLYHATLILYSLGKAGKPQYARKVF-- 495
             Y  +I  L +S    +  ++   +  +G    +   T ++    KAGK   A  +   
Sbjct: 519 WTYTSMIDSLCKSKRVEEACDLFDSLEQKGVNPNVVMYTALIDGYCKAGKVDEAHLMLEK 578

Query: 496 ----NMLPEELKCTATYTALVDGYFSAGSSGKGLKIFETMRKKGFTPSLGTYNVLLNGLA 547
               N LP  L    T+ AL+ G  + G   +   + E M K G  P++ T  +L++ L 
Sbjct: 579 MLSKNCLPNSL----TFNALIHGLCADGKLKEATLLEEKMVKIGLQPTVSTDTILIHRLL 616

HSP 3 Score: 80.9 bits (198), Expect = 2.9e-15
Identity = 99/415 (23.86%), Postives = 164/415 (39.52%), Query Frame = 1

Query: 137 TYTTMLDIFGEAGRISSMNYVFQQMKEKGIKIDAVTYTSLMHWRSNSGDVDGAIKVWKEM 196
           TYT ++     + R S    + ++M+E GIK +  TYT L+    +    + A ++  +M
Sbjct: 325 TYTVLIKSLCGSERKSEALNLVKEMEETGIKPNIHTYTVLIDSLCSQCKFEKARELLGQM 384

Query: 197 KANGCHPTVVSYTAYIKILLDNGQINEATATYKKMLQSGLSPNCCTYTILMEYLIGEGKC 256
              G  P V++Y A I      G I +A    + M    LSPN  TY  L+     +G C
Sbjct: 385 LEKGLMPNVITYNALINGYCKRGMIEDAVDVVELMESRKLSPNTRTYNELI-----KGYC 444

Query: 257 K----EALDIFSKMQDAGVYPDKAACNILIQKCCKSGERLVMTQILEFMKENRFVLRYPV 316
           K    +A+ + +KM +  V PD    N LI   C+SG      ++L  M +   V     
Sbjct: 445 KSNVHKAMGVLNKMLERKVLPDVVTYNSLIDGQCRSGNFDSAYRLLSLMNDRGLVPDQWT 504

Query: 317 FVEAHETL-KSCSVSYALLKQVNPHMEIESISKGEVVDVSTGSNTVPPNV--DNELLAML 376
           +    ++L KS  V  A                 ++ D S     V PNV     L+   
Sbjct: 505 YTSMIDSLCKSKRVEEAC----------------DLFD-SLEQKGVNPNVVMYTALIDGY 564

Query: 377 LKDNKLTAVDHMLIGIVDKNIQLDSSIIYSIIEVNCKSNRPNSALLAFDYCLKNSVNIKR 436
            K  K+     ML  ++ KN   +S    ++I   C   +   A L  +  +K  +    
Sbjct: 565 CKAGKVDEAHLMLEKMLSKNCLPNSLTFNALIHGLCADGKLKEATLLEEKMVKIGLQPTV 624

Query: 437 KLYLDLIGILIRSSIYPKLLEIVQEMYTQGHCLGLYHATLILYSLGKAGKPQYARKVFNM 496
                LI  L++   +       Q+M + G     +  T  + +  + G+   A  +   
Sbjct: 625 STDTILIHRLLKDGDFDHAYSRFQQMLSSGTKPDAHTYTTFIQTYCREGRLLDAEDMMAK 684

Query: 497 LPEE--LKCTATYTALVDGYFSAGSSGKGLKIFETMRKKGFTPSLGTYNVLLNGL 543
           + E        TY++L+ GY   G +     + + MR  G  PS  T+  L+  L
Sbjct: 685 MRENGVSPDLFTYSSLIKGYGDLGQTNFAFDVLKRMRDTGCEPSQHTFLSLIKHL 717

BLAST of Cucsa.250610 vs. TAIR10
Match: AT4G31850.1 (AT4G31850.1 proton gradient regulation 3)

HSP 1 Score: 120.2 bits (300), Expect = 4.4e-27
Identity = 112/422 (26.54%), Postives = 190/422 (45.02%), Query Frame = 1

Query: 134 DQYTYTTMLDIFGEAGRISSMNYVFQQMKEKGIKIDAVTYTSLMHWRSNSGDVDGAIKVW 193
           D  TY  M+  + + G I     +  +M E G + D +   SL++    +  VD A K++
Sbjct: 502 DSVTYNMMMKCYSKVGEIDEAIKLLSEMMENGCEPDVIVVNSLINTLYKADRVDEAWKMF 561

Query: 194 KEMKANGCHPTVVSYTAYIKILLDNGQINEATATYKKMLQSGLSPNCCTYTILMEYLIGE 253
             MK     PTVV+Y   +  L  NG+I EA   ++ M+Q G  PN  T+  L + L   
Sbjct: 562 MRMKEMKLKPTVVTYNTLLAGLGKNGKIQEAIELFEGMVQKGCPPNTITFNTLFDCLCKN 621

Query: 254 GKCKEALDIFSKMQDAGVYPDKAACNILIQKCCKSGERLVMTQILEFMKENRFVLRYPVF 313
            +   AL +  KM D G  PD    N +I    K+G+          MK+    L YP F
Sbjct: 622 DEVTLALKMLFKMMDMGCVPDVFTYNTIIFGLVKNGQVKEAMCFFHQMKK----LVYPDF 681

Query: 314 VEAHETLKSCSVSYALLKQVNPHMEIESISKGEVVDVSTGSNTVPPNVDNELLAMLLKDN 373
           V    TL  C++   ++K        + I+          +N    ++   +LA    DN
Sbjct: 682 V----TL--CTLLPGVVKASLIEDAYKIITNFLYNCADQPANLFWEDLIGSILAEAGIDN 741

Query: 374 KLTAVDHMLIGIVDKNIQLDSSIIYSIIEVNCKSNRPNSALLAFDYCLKN-SVNIKRKLY 433
            ++  + ++   + ++     SI+  II  +CK N  + A   F+   K+  V  K   Y
Sbjct: 742 AVSFSERLVANGICRD---GDSILVPIIRYSCKHNNVSGARTLFEKFTKDLGVQPKLPTY 801

Query: 434 LDLIGILIRSSIYPKLLEIVQEMYTQGHCLG----LYHATLILYSLGKAGKPQYARKVF- 493
             LIG L+ +     ++EI Q+++ Q    G    +     +L + GK+GK     +++ 
Sbjct: 802 NLLIGGLLEAD----MIEIAQDVFLQVKSTGCIPDVATYNFLLDAYGKSGKIDELFELYK 861

Query: 494 NMLPEELKC-TATYTALVDGYFSAGSSGKGLKI-FETMRKKGFTPSLGTYNVLLNGLAKN 548
            M   E +  T T+  ++ G   AG+    L + ++ M  + F+P+  TY  L++GL+K+
Sbjct: 862 EMSTHECEANTITHNIVISGLVKAGNVDDALDLYYDLMSDRDFSPTACTYGPLIDGLSKS 906

HSP 2 Score: 114.0 bits (284), Expect = 3.1e-25
Identity = 108/434 (24.88%), Postives = 184/434 (42.40%), Query Frame = 1

Query: 129 QVFKHDQYTYTTMLDIFGEAGRISSMNYVFQQMKEKGIKIDAVTYTSLMHWRSNSGDVDG 188
           ++ K D  TY T+       G +    Y  ++M+E G  ++A +Y  L+H    S     
Sbjct: 147 RIIKRDTNTYLTIFKSLSVKGGLKQAPYALRKMREFGFVLNAYSYNGLIHLLLKSRFCTE 206

Query: 189 AIKVWKEMKANGCHPTVVSYTAYIKILLDNGQINEATATYKKMLQSGLSPNCCTYTILME 248
           A++V++ M   G  P++ +Y++ +  L     I+      K+M   GL PN  T+TI + 
Sbjct: 207 AMEVYRRMILEGFRPSLQTYSSLMVGLGKRRDIDSVMGLLKEMETLGLKPNVYTFTICIR 266

Query: 249 YLIGEGKCKEALDIFSKMQDAGVYPDKAACNILIQKCCKSGERLVMTQILEFMKENRFVL 308
            L   GK  EA +I  +M D G  PD     +LI   C + +     ++ E MK  R   
Sbjct: 267 VLGRAGKINEAYEILKRMDDEGCGPDVVTYTVLIDALCTARKLDCAKEVFEKMKTGRH-- 326

Query: 309 RYPVFVEAHETLKSCSVSY-ALLKQVNPHMEIESISK--------GEVVDVSTGSNTVPP 368
                       K   V+Y  LL + + + +++S+ +        G V DV T +  V  
Sbjct: 327 ------------KPDRVTYITLLDRFSDNRDLDSVKQFWSEMEKDGHVPDVVTFTILVD- 386

Query: 369 NVDNELLAMLLKDNKLTAVDHMLI----GIVDKNIQLDSSIIYSIIEVNCKSNRPNSALL 428
                  A+    N   A D + +    GI+  N+   +++I  ++ V    +R + AL 
Sbjct: 387 -------ALCKAGNFGEAFDTLDVMRDQGIL-PNLHTYNTLICGLLRV----HRLDDALE 446

Query: 429 AFDYCLKNSVNIKRKLYLDLIGILIRSSIYPKLLEIVQEMYTQGHCLGLYHATLILYSLG 488
            F       V      Y+  I    +S      LE  ++M T+G    +      LYSL 
Sbjct: 447 LFGNMESLGVKPTAYTYIVFIDYYGKSGDSVSALETFEKMKTKGIAPNIVACNASLYSLA 506

Query: 489 KAGKPQYARKVFNMLPE--ELKCTATYTALVDGYFSAGSSGKGLKIFETMRKKGFTPSLG 548
           KAG+ + A+++F  L +   +  + TY  ++  Y   G   + +K+   M + G  P + 
Sbjct: 507 KAGRDREAKQIFYGLKDIGLVPDSVTYNMMMKCYSKVGEIDEAIKLLSEMMENGCEPDVI 553

HSP 3 Score: 99.4 bits (246), Expect = 8.0e-21
Identity = 102/450 (22.67%), Postives = 183/450 (40.67%), Query Frame = 1

Query: 132 KHDQYTYTTMLDIFGEAGRISSMNYVFQQMKEKGIKIDAVTYTSLMHWRSNSGDVDGAIK 191
           K + YT+T  + + G AG+I+    + ++M ++G   D VTYT L+     +  +D A +
Sbjct: 255 KPNVYTFTICIRVLGRAGKINEAYEILKRMDDEGCGPDVVTYTVLIDALCTARKLDCAKE 314

Query: 192 VWKEMKANGCHPTVVSYTAYIKILLDNGQINEATATYKKMLQSGLSPNCCTYTILMEYLI 251
           V+++MK     P  V+Y   +    DN  ++     + +M + G  P+  T+TIL++ L 
Sbjct: 315 VFEKMKTGRHKPDRVTYITLLDRFSDNRDLDSVKQFWSEMEKDGHVPDVVTFTILVDALC 374

Query: 252 GEGKCKEALDIFSKMQDAGVYPDKAACNILI---QKCCKSGERLVMTQILEFMKENRFVL 311
             G   EA D    M+D G+ P+    N LI    +  +  + L +   +E +       
Sbjct: 375 KAGNFGEAFDTLDVMRDQGILPNLHTYNTLICGLLRVHRLDDALELFGNMESLGVKPTAY 434

Query: 312 RYPVFVEAHETLKSCSVSYALLKQVNPHMEIESISKGEVVDVSTGSNTVPPNV--DNELL 371
            Y VF++ +             K  +    +E+  K +       +  + PN+   N  L
Sbjct: 435 TYIVFIDYYG------------KSGDSVSALETFEKMK-------TKGIAPNIVACNASL 494

Query: 372 AMLLKDNKLTAVDHMLIGIVDKNIQLDSSIIYSIIEVNCKSNRPNSALLAFDYCLKNSVN 431
             L K  +      +  G+ D  +  DS     +++   K    + A+      ++N   
Sbjct: 495 YSLAKAGRDREAKQIFYGLKDIGLVPDSVTYNMMMKCYSKVGEIDEAIKLLSEMMENGCE 554

Query: 432 IKRKLYLDLIGILIRSSIYPKLLEIVQEMYTQGHCLGLYHATLILYSLGKAGKPQYARKV 491
               +   LI  L ++    +  ++   M        +     +L  LGK GK Q A ++
Sbjct: 555 PDVIVVNSLINTLYKADRVDEAWKMFMRMKEMKLKPTVVTYNTLLAGLGKNGKIQEAIEL 614

Query: 492 FNMLPEELKC---TATYTALVDGYFSAGSSGKGLKIFETMRKKGFTPSLGTYNVLLNGLA 551
           F  + ++  C   T T+  L D           LK+   M   G  P + TYN ++ GL 
Sbjct: 615 FEGMVQK-GCPPNTITFNTLFDCLCKNDEVTLALKMLFKMMDMGCVPDVFTYNTIIFGLV 674

Query: 552 KNGRGVELNIYRREKKSFEISHHSRLNTIL 574
           KNG+  E   +  + K         L T+L
Sbjct: 675 KNGQVKEAMCFFHQMKKLVYPDFVTLCTLL 684

HSP 4 Score: 85.9 bits (211), Expect = 9.2e-17
Identity = 108/486 (22.22%), Postives = 202/486 (41.56%), Query Frame = 1

Query: 104  LINQVLKTHPPLEKTWLFFNWASTLQVFKHDQYTYTTMLDIFGEAGRISSMNYVFQQMKE 163
            LIN + K    +++ W  F     +++ K    TY T+L   G+ G+I     +F+ M +
Sbjct: 544  LINTLYKADR-VDEAWKMFMRMKEMKL-KPTVVTYNTLLAGLGKNGKIQEAIELFEGMVQ 603

Query: 164  KGIKIDAVTYTSLMHWRSNSGDVDGAIKVWKEMKANGCHPTVVSYTAYIKILLDNGQINE 223
            KG   + +T+ +L      + +V  A+K+  +M   GC P V +Y   I  L+ NGQ+ E
Sbjct: 604  KGCPPNTITFNTLFDCLCKNDEVTLALKMLFKMMDMGCVPDVFTYNTIIFGLVKNGQVKE 663

Query: 224  ATATYKKMLQSGLSPNCCTYTILMEYLIGEGKCKEALDIFSKM------QDAGVYPDKAA 283
            A   + +M +  + P+  T   L+  ++     ++A  I +        Q A ++ +   
Sbjct: 664  AMCFFHQM-KKLVYPDFVTLCTLLPGVVKASLIEDAYKIITNFLYNCADQPANLFWEDLI 723

Query: 284  CNILIQK----------------CCKSGERLVMTQILEFMKENRFVLRYPVFVEAHETL- 343
             +IL +                  C+ G+ +++  I    K N       +F +  + L 
Sbjct: 724  GSILAEAGIDNAVSFSERLVANGICRDGDSILVPIIRYSCKHNNVSGARTLFEKFTKDLG 783

Query: 344  -KSCSVSYALLKQVNPHMEIESISKGEVVDVSTGSNTVPPNVD--NELLAMLLKDNKLTA 403
             +    +Y LL  +   +E + I   + V +   S    P+V   N LL    K  K+  
Sbjct: 784  VQPKLPTYNLL--IGGLLEADMIEIAQDVFLQVKSTGCIPDVATYNFLLDAYGKSGKIDE 843

Query: 404  VDHMLIGIVDKNIQLDSSIIYSIIEVNCKSNRPNSAL-LAFDYCLKNSVNIKRKLYLDLI 463
            +  +   +     + ++     +I    K+   + AL L +D       +     Y  LI
Sbjct: 844  LFELYKEMSTHECEANTITHNIVISGLVKAGNVDDALDLYYDLMSDRDFSPTACTYGPLI 903

Query: 464  GILIRSSIYPKLLEIVQEMYTQGHCLGLYHATLILYSLGKAGKPQYARKVFNMLPEE--L 523
              L +S    +  ++ + M   G         +++   GKAG+   A  +F  + +E   
Sbjct: 904  DGLSKSGRLYEAKQLFEGMLDYGCRPNCAIYNILINGFGKAGEADAACALFKRMVKEGVR 963

Query: 524  KCTATYTALVDGYFSAGSSGKGLKIFETMRKKGFTPSLGTYNVLLNGLAKNGRGVELNIY 561
                TY+ LVD     G   +GL  F+ +++ G  P +  YN+++NGL K+ R  E  + 
Sbjct: 964  PDLKTYSVLVDCLCMVGRVDEGLHYFKELKESGLNPDVVCYNLIINGLGKSHRLEEALVL 1023

HSP 5 Score: 81.3 bits (199), Expect = 2.3e-15
Identity = 104/424 (24.53%), Postives = 174/424 (41.04%), Query Frame = 1

Query: 136 YTYTTMLDIFGEAGRISSMNYVFQQMKEKGIKIDAVTYTSLMHWRSNSGDVDGAIKVWKE 195
           +TY T++       R+     +F  M+  G+K  A TY   + +   SGD   A++ +++
Sbjct: 399 HTYNTLICGLLRVHRLDDALELFGNMESLGVKPTAYTYIVFIDYYGKSGDSVSALETFEK 458

Query: 196 MKANGCHPTVVSYTAYIKILLDNGQINEATATYKKMLQSGLSPNCCTYTILMEYLIGEGK 255
           MK  G  P +V+  A +  L   G+  EA   +  +   GL P+  TY ++M+     G+
Sbjct: 459 MKTKGIAPNIVACNASLYSLAKAGRDREAKQIFYGLKDIGLVPDSVTYNMMMKCYSKVGE 518

Query: 256 CKEALDIFSKMQDAGVYPDKAACNILIQKCCKSGERLVMTQILEFMKENRFVLRYPVFVE 315
             EA+ + S+M + G  PD    N LI    K+       ++   MKE +          
Sbjct: 519 IDEAIKLLSEMMENGCEPDVIVVNSLINTLYKADRVDEAWKMFMRMKEMK---------- 578

Query: 316 AHETLKSCSVSY-ALLKQVNPHMEIESISKGEVVDVSTG--SNTVPPN--VDNELLAMLL 375
               LK   V+Y  LL  +  + +I+     E +++  G      PPN    N L   L 
Sbjct: 579 ----LKPTVVTYNTLLAGLGKNGKIQ-----EAIELFEGMVQKGCPPNTITFNTLFDCLC 638

Query: 376 KDNKLTAVDHMLIGIVDKNIQLDSSIIYSIIEVNCKSNRPNSALLAFDYCLKNSVNIKRK 435
           K++++T    ML  ++D     D     +II    K+ +   A+  F         +K+ 
Sbjct: 639 KNDEVTLALKMLFKMMDMGCVPDVFTYNTIIFGLVKNGQVKEAMCFFH-------QMKKL 698

Query: 436 LYLDLIGI--LIRSSIYPKLLEIVQEMYTQ------GHCLGLYHATLILYSLGKAGKPQY 495
           +Y D + +  L+   +   L+E   ++ T            L+   LI   L +AG    
Sbjct: 699 VYPDFVTLCTLLPGVVKASLIEDAYKIITNFLYNCADQPANLFWEDLIGSILAEAGIDN- 758

Query: 496 ARKVFNMLPEELKCTATYTALVD--GYFSAGSSGKGLK-IFETMRKK-GFTPSLGTYNVL 543
           A      L     C    + LV    Y    ++  G + +FE   K  G  P L TYN+L
Sbjct: 759 AVSFSERLVANGICRDGDSILVPIIRYSCKHNNVSGARTLFEKFTKDLGVQPKLPTYNLL 795

BLAST of Cucsa.250610 vs. TAIR10
Match: AT1G18900.3 (AT1G18900.3 Pentatricopeptide repeat (PPR) superfamily protein)

HSP 1 Score: 117.9 bits (294), Expect = 2.2e-26
Identity = 76/246 (30.89%), Postives = 120/246 (48.78%), Query Frame = 1

Query: 46  SRFSRQSRKGTKVAKKEEVIPRLYTRD--TVRNICNILRNCSWA-SAQKHLEMLPIRWDS 105
           S FS  S +  K      +  R Y      V N+ ++LR   W  +A++ L+ L +R D+
Sbjct: 272 SGFSNSSVEMMKGPSGTALTSRQYCNSGHIVENVSSVLRRFRWGPAAEEALQNLGLRIDA 331

Query: 106 YLINQVLKTHPPLEKTWLFFNWASTLQVFKHDQYTYTTMLDIFGEAGRISSMNYVFQQMK 165
           Y  NQVLK          FF W      FKHD +TYTTM+   G A +  ++N +  +M 
Sbjct: 332 YQANQVLKQMNDYGNALGFFYWLKRQPGFKHDGHTYTTMVGNLGRAKQFGAINKLLDEMV 391

Query: 166 EKGIKIDAVTYTSLMHWRSNSGDVDGAIKVWKEMKANGCHPTVVSYTAYIKILLDNGQIN 225
             G + + VTY  L+H    +  ++ A+ V+ +M+  GC P  V+Y   I I    G ++
Sbjct: 392 RDGCQPNTVTYNRLIHSYGRANYLNEAMNVFNQMQEAGCKPDRVTYCTLIDIHAKAGFLD 451

Query: 226 EATATYKKMLQSGLSPNCCTYTILMEYLIGEGKCKEALDIFSKMQDAGVYPDKAACNILI 285
            A   Y++M   GLSP+  TY++++  L   G    A  +F +M D G  P+    NI++
Sbjct: 452 IAMDMYQRMQAGGLSPDTFTYSVIINCLGKAGHLPAAHKLFCEMVDQGCTPNLVTYNIMM 511

Query: 286 QKCCKS 289
               K+
Sbjct: 512 DLHAKA 517

HSP 2 Score: 114.8 bits (286), Expect = 1.8e-25
Identity = 84/306 (27.45%), Postives = 134/306 (43.79%), Query Frame = 1

Query: 46  SRFSRQSRKGTKVAKKEEVIPRLYTRD--TVRNICNILRNCSWA-SAQKHLEMLPIRWDS 105
           S FS  S +  K      +  R Y      V N+ ++LR   W  +A++ L+ L +R D+
Sbjct: 272 SGFSNSSVEMMKGPSGTALTSRQYCNSGHIVENVSSVLRRFRWGPAAEEALQNLGLRIDA 331

Query: 106 YLINQVLKTHPPLEKTWLFFNWASTLQVFKHDQYTYTTMLDIFGEAGRISSMN------- 165
           Y  NQVLK          FF W      FKHD +TYTTM+   G A +  ++N       
Sbjct: 332 YQANQVLKQMNDYGNALGFFYWLKRQPGFKHDGHTYTTMVGNLGRAKQFGAINKLLDEMV 391

Query: 166 ----------------------------YVFQQMKEKGIKIDAVTYTSLMHWRSNSGDVD 225
                                        VF QM+E G K D VTY +L+   + +G +D
Sbjct: 392 RDGCQPNTVTYNRLIHSYGRANYLNEAMNVFNQMQEAGCKPDRVTYCTLIDIHAKAGFLD 451

Query: 226 GAIKVWKEMKANGCHPTVVSYTAYIKILLDNGQINEATATYKKMLQSGLSPNCCTYTILM 285
            A+ +++ M+A G  P   +Y+  I  L   G +  A   + +M+  G +PN  TY I+M
Sbjct: 452 IAMDMYQRMQAGGLSPDTFTYSVIINCLGKAGHLPAAHKLFCEMVDQGCTPNLVTYNIMM 511

Query: 286 EYLIGEGKCKEALDIFSKMQDAGVYPDKAACNILIQKCCKSGERLVMTQILEFMKENRFV 314
           +        + AL ++  MQ+AG  PDK   +I+++     G       +   M++  ++
Sbjct: 512 DLHAKARNYQNALKLYRDMQNAGFEPDKVTYSIVMEVLGHCGYLEEAEAVFTEMQQKNWI 571

HSP 3 Score: 64.7 bits (156), Expect = 2.2e-10
Identity = 40/153 (26.14%), Postives = 69/153 (45.10%), Query Frame = 1

Query: 137 TYTTMLDIFGEAGRISSMNYVFQQMKEKGIKIDAVTYTSLMHWRSNSGDVDGAIKVWKEM 196
           TY  M+D+  +A    +   +++ M+  G + D VTY+ +M    + G ++ A  V+ EM
Sbjct: 506 TYNIMMDLHAKARNYQNALKLYRDMQNAGFEPDKVTYSIVMEVLGHCGYLEEAEAVFTEM 565

Query: 197 KANGCHPTVVSYTAYIKILLDNGQINEATATYKKMLQSGLSPNCCTYTILMEYLIGEGKC 256
           +     P    Y   + +    G + +A   Y+ ML +GL PN  T   L+   +   K 
Sbjct: 566 QQKNWIPDEPVYGLLVDLWGKAGNVEKAWQWYQAMLHAGLRPNVPTCNSLLSTFLRVNKI 625

Query: 257 KEALDIFSKMQDAGVYPDKAACNILIQKCCKSG 290
            EA ++   M   G+ P      +L+  CC  G
Sbjct: 626 AEAYELLQNMLALGLRPSLQTYTLLL-SCCTDG 657

HSP 4 Score: 32.7 bits (73), Expect = 9.2e-01
Identity = 21/61 (34.43%), Postives = 34/61 (55.74%), Query Frame = 1

Query: 497 TATYTALVDGYFSAGSSGKGLKIFETMRKKGFTPSLGTYNVL--LNGLAKNGRGVELNIY 556
           T TY+ +++    AG      K+F  M  +G TP+L TYN++  L+  A+N +   L +Y
Sbjct: 469 TFTYSVIINCLGKAGHLPAAHKLFCEMVDQGCTPNLVTYNIMMDLHAKARNYQNA-LKLY 528

BLAST of Cucsa.250610 vs. NCBI nr
Match: gi|778670108|ref|XP_011649371.1| (PREDICTED: pentatricopeptide repeat-containing protein At2g01390-like [Cucumis sativus])

HSP 1 Score: 1181.0 bits (3054), Expect = 0.0e+00
Identity = 586/588 (99.66%), Postives = 587/588 (99.83%), Query Frame = 1

Query: 1   MHFCNIFSLLLSNYVVSSAIRKRIYQNISSKCLHSLHQYKRDKPISRFSRQSRKGTKVAK 60
           MHFCNIFSLLLSNYVVSSAIRKRIYQNISSKCLHSLHQYKRDKPISRFSRQSRKGTKVAK
Sbjct: 1   MHFCNIFSLLLSNYVVSSAIRKRIYQNISSKCLHSLHQYKRDKPISRFSRQSRKGTKVAK 60

Query: 61  KEEVIPRLYTRDTVRNICNILRNCSWASAQKHLEMLPIRWDSYLINQVLKTHPPLEKTWL 120
           KEEVIPRLYTRDTVRNICNILRNCSWASAQKHLEMLPIRWDSYLINQVLKTHPPLEKTWL
Sbjct: 61  KEEVIPRLYTRDTVRNICNILRNCSWASAQKHLEMLPIRWDSYLINQVLKTHPPLEKTWL 120

Query: 121 FFNWASTLQVFKHDQYTYTTMLDIFGEAGRISSMNYVFQQMKEKGIKIDAVTYTSLMHWR 180
           FFNWASTLQVFKHDQYTYTTMLDIFGEAGRISSMNYVFQQMKEKGIKIDAVTYTSLMHWR
Sbjct: 121 FFNWASTLQVFKHDQYTYTTMLDIFGEAGRISSMNYVFQQMKEKGIKIDAVTYTSLMHWR 180

Query: 181 SNSGDVDGAIKVWKEMKANGCHPTVVSYTAYIKILLDNGQINEATATYKKMLQSGLSPNC 240
           SNSGDVDGAIK+WKEMKANGCHPTVVSYTAYIKILLDNGQINEATATYKKMLQSGLSPNC
Sbjct: 181 SNSGDVDGAIKLWKEMKANGCHPTVVSYTAYIKILLDNGQINEATATYKKMLQSGLSPNC 240

Query: 241 CTYTILMEYLIGEGKCKEALDIFSKMQDAGVYPDKAACNILIQKCCKSGERLVMTQILEF 300
           CTYTILMEYLIGEGKCKEALDIFSKMQDAGVYPDKAACNILIQKCCKSGERLVMTQILEF
Sbjct: 241 CTYTILMEYLIGEGKCKEALDIFSKMQDAGVYPDKAACNILIQKCCKSGERLVMTQILEF 300

Query: 301 MKENRFVLRYPVFVEAHETLKSCSVSYALLKQVNPHMEIESISKGEVVDVSTGSNTVPPN 360
           MKENRFVLRYPVFVEAHETLKSCSVSYALLKQVNPHMEIESISKGEVVDVSTGSNTVPPN
Sbjct: 301 MKENRFVLRYPVFVEAHETLKSCSVSYALLKQVNPHMEIESISKGEVVDVSTGSNTVPPN 360

Query: 361 VDNELLAMLLKDNKLTAVDHMLIGIVDKNIQLDSSIIYSIIEVNCKSNRPNSALLAFDYC 420
           VDNELLAMLLKDNKLTAVDHMLIGIVDKNIQLDSSIIYSIIEVNCKSNRPNSALLAFDYC
Sbjct: 361 VDNELLAMLLKDNKLTAVDHMLIGIVDKNIQLDSSIIYSIIEVNCKSNRPNSALLAFDYC 420

Query: 421 LKNSVNIKRKLYLDLIGILIRSSIYPKLLEIVQEMYTQGHCLGLYHATLILYSLGKAGKP 480
           LKNSVNIKRKLYLDLIGILIRSSIYPKLLEIVQEMYTQGHCLGLYHATLIL SLGKAGKP
Sbjct: 421 LKNSVNIKRKLYLDLIGILIRSSIYPKLLEIVQEMYTQGHCLGLYHATLILCSLGKAGKP 480

Query: 481 QYARKVFNMLPEELKCTATYTALVDGYFSAGSSGKGLKIFETMRKKGFTPSLGTYNVLLN 540
           QYARKVFNMLPEELKCTATYTALVDGYFSAGSSGKGLKIFETMRKKGFTPSLGTYNVLLN
Sbjct: 481 QYARKVFNMLPEELKCTATYTALVDGYFSAGSSGKGLKIFETMRKKGFTPSLGTYNVLLN 540

Query: 541 GLAKNGRGVELNIYRREKKSFEISHHSRLNTILDDERICDLLFGELVS 589
           GLAKNGRGVELNIYRREKKSFEISHHSRLNTILDDERICDLLFGELVS
Sbjct: 541 GLAKNGRGVELNIYRREKKSFEISHHSRLNTILDDERICDLLFGELVS 588

BLAST of Cucsa.250610 vs. NCBI nr
Match: gi|659116058|ref|XP_008457878.1| (PREDICTED: pentatricopeptide repeat-containing protein At2g01390-like [Cucumis melo])

HSP 1 Score: 1130.5 bits (2923), Expect = 0.0e+00
Identity = 555/588 (94.39%), Postives = 573/588 (97.45%), Query Frame = 1

Query: 1   MHFCNIFSLLLSNYVVSSAIRKRIYQNISSKCLHSLHQYKRDKPISRFSRQSRKGTKVAK 60
           MHFCN FSLLLSNYVV SAIRKRIYQNIS KCLHSLHQYKR+KPISRFSR SRKGTKV K
Sbjct: 1   MHFCNRFSLLLSNYVVISAIRKRIYQNISCKCLHSLHQYKREKPISRFSRNSRKGTKVVK 60

Query: 61  KEEVIPRLYTRDTVRNICNILRNCSWASAQKHLEMLPIRWDSYLINQVLKTHPPLEKTWL 120
           KEEVIPR+YTRDTV NICNILRNCSWASAQKHLEMLPIRWDSYLINQVLKTHPPLEKTWL
Sbjct: 61  KEEVIPRVYTRDTVCNICNILRNCSWASAQKHLEMLPIRWDSYLINQVLKTHPPLEKTWL 120

Query: 121 FFNWASTLQVFKHDQYTYTTMLDIFGEAGRISSMNYVFQQMKEKGIKIDAVTYTSLMHWR 180
           FFNWAS L+VFKHDQYTYTTMLDIFGEAGRISSMNY+FQQMKEKGIKIDA TYTSLMHWR
Sbjct: 121 FFNWASRLKVFKHDQYTYTTMLDIFGEAGRISSMNYLFQQMKEKGIKIDAATYTSLMHWR 180

Query: 181 SNSGDVDGAIKVWKEMKANGCHPTVVSYTAYIKILLDNGQINEATATYKKMLQSGLSPNC 240
           SNSGDVDGAIKVWKEMKANGCHPTVVSYTAYIKILLDNGQ  EATATYK+ML++GLSPNC
Sbjct: 181 SNSGDVDGAIKVWKEMKANGCHPTVVSYTAYIKILLDNGQSKEATATYKEMLKTGLSPNC 240

Query: 241 CTYTILMEYLIGEGKCKEALDIFSKMQDAGVYPDKAACNILIQKCCKSGERLVMTQILEF 300
           CTYTILMEYLIGEGKCKEALDIFSKMQDAGVYPDKAACNILIQKCCKSGERLVMTQILEF
Sbjct: 241 CTYTILMEYLIGEGKCKEALDIFSKMQDAGVYPDKAACNILIQKCCKSGERLVMTQILEF 300

Query: 301 MKENRFVLRYPVFVEAHETLKSCSVSYALLKQVNPHMEIESISKGEVVDVSTGSNTVPPN 360
           MKENRFVLRYPVFVEAHE LKSCSV +ALL+QVNPH+EIESISKGEV+DVSTGSNTVPPN
Sbjct: 301 MKENRFVLRYPVFVEAHENLKSCSVGHALLRQVNPHIEIESISKGEVLDVSTGSNTVPPN 360

Query: 361 VDNELLAMLLKDNKLTAVDHMLIGIVDKNIQLDSSIIYSIIEVNCKSNRPNSALLAFDYC 420
           VDNELLAMLLKDNKLTA+DHMLIGIVDKNIQLDSSIIYSIIEVNCKSNRPNSA+LAFDYC
Sbjct: 361 VDNELLAMLLKDNKLTAIDHMLIGIVDKNIQLDSSIIYSIIEVNCKSNRPNSAMLAFDYC 420

Query: 421 LKNSVNIKRKLYLDLIGILIRSSIYPKLLEIVQEMYTQGHCLGLYHATLILYSLGKAGKP 480
           LKN VNI RKLYLDLIGILIRSSIYPKLLEIVQEMYTQGHC+GLYHATLILYSLG+AGKP
Sbjct: 421 LKNGVNIGRKLYLDLIGILIRSSIYPKLLEIVQEMYTQGHCIGLYHATLILYSLGRAGKP 480

Query: 481 QYARKVFNMLPEELKCTATYTALVDGYFSAGSSGKGLKIFETMRKKGFTPSLGTYNVLLN 540
           QYARKVFN+LPEELKCTATYT+LVD YFSAGSSGKGLKIFETMRKKGFTPSLGTYNVLLN
Sbjct: 481 QYARKVFNILPEELKCTATYTSLVDAYFSAGSSGKGLKIFETMRKKGFTPSLGTYNVLLN 540

Query: 541 GLAKNGRGVELNIYRREKKSFEISHHSRLNTILDDERICDLLFGELVS 589
           GLAK+GRGVELNIYRREKKSFEISHHSRLNTILDDERICDLLFGELVS
Sbjct: 541 GLAKSGRGVELNIYRREKKSFEISHHSRLNTILDDERICDLLFGELVS 588

BLAST of Cucsa.250610 vs. NCBI nr
Match: gi|802640946|ref|XP_012079092.1| (PREDICTED: pentatricopeptide repeat-containing protein At2g01390 [Jatropha curcas])

HSP 1 Score: 642.9 bits (1657), Expect = 5.5e-181
Identity = 321/585 (54.87%), Postives = 427/585 (72.99%), Query Frame = 1

Query: 1   MHFCNIFSLLLSNYVVSSAIR--KRIYQNISSKCLHSLHQYKRDKPISRFSRQSRKGTKV 60
           +HF  I   L+ + + SS+       Y +IS KC HS HQ    K I +F++   K   +
Sbjct: 15  LHFHGINRFLIKSLISSSSYDGWPFRYFSISGKCFHSFHQSTPRKSIKQFTKTPEKAQNI 74

Query: 61  AKKEEVIPRLYTRDTVRNICNILRNCSWASAQKHLEMLPIRWDSYLINQVLKTHPPLEKT 120
                  P +Y R+T+ NI  IL+  SW +A++HL  L I+WDS+ +NQVLKTHPP+EK 
Sbjct: 75  ED-----PNIYMRNTISNIYKILKYSSWDTAKEHLSKLGIKWDSFTVNQVLKTHPPMEKA 134

Query: 121 WLFFNWASTLQVFKHDQYTYTTMLDIFGEAGRISSMNYVFQQMKEKGIKIDAVTYTSLMH 180
           WLFFNWAS L+ FKHDQ+TYTTMLDIFGEAGRI+SMNYVF+QM+EKGIKIDA TYTSLMH
Sbjct: 135 WLFFNWASGLKGFKHDQFTYTTMLDIFGEAGRIASMNYVFKQMQEKGIKIDAATYTSLMH 194

Query: 181 WRSNSGDVDGAIKVWKEMKANGCHPTVVSYTAYIKILLDNGQINEATATYKKMLQSGLSP 240
             S+SGD+DGAIK+W+EMK NGC P+VVSYTA++K+L D+ ++ EAT  Y +ML+SG+SP
Sbjct: 195 RVSSSGDIDGAIKIWEEMKENGCFPSVVSYTAFMKVLFDSNRVKEATDVYTEMLESGISP 254

Query: 241 NCCTYTILMEYLIGEGKCKEALDIFSKMQDAGVYPDKAACNILIQKCCKSGERLVMTQIL 300
           NC TYT+LMEYLI  GK +EAL+IF KMQ+AGV PDKA CNIL++KCCK+GE   MTQIL
Sbjct: 255 NCYTYTVLMEYLIVAGKYQEALEIFRKMQEAGVQPDKALCNILVEKCCKAGETNTMTQIL 314

Query: 301 EFMKENRFVLRYPVFVEAHETLKSCSVSYALLKQVNPHMEIESISKGEVVDVSTGSNTVP 360
           ++MK N   LRYP+F+EA  TL+    S ALL+QVNPH   ESI   +  +    +   P
Sbjct: 315 QYMKSNCLALRYPIFLEALRTLRIAGGSDALLRQVNPHCSTESIKNCDAPEFLASAAEDP 374

Query: 361 PNVDNELLAMLLKDNKLTAVDHMLIGIVDKNIQLDSSIIYSIIEVNCKSNRPNSALLAFD 420
             ++  LL +LLK   L AVDH++ GI+DKNI LDS ++ +IIEVN    RP+SALLAF+
Sbjct: 375 --LEQGLLLILLKKQNLVAVDHLIAGIMDKNILLDSWVVSTIIEVNSTQCRPDSALLAFE 434

Query: 421 YCLKNSVNIKRKLYLDLIGILIRSSIYPKLLEIVQEMYTQGHCLGLYHATLILYSLGKAG 480
           Y  +  +++ R +YL LIGILIRS+ +P+++E+V+EM   GH LGLY + L++Y LG A 
Sbjct: 435 YSTRMGIDLDRTVYLSLIGILIRSNTFPRVVEVVKEMIRAGHSLGLYLSALLIYRLGSAR 494

Query: 481 KPQYARKVFNMLPEELKCTATYTALVDGYFSAGSSGKGLKIFETMRKKGFTPSLGTYNVL 540
           +P +A K+FN+LP E KCTAT+TA++  YFSAGS+ K LKI++TM+KKG  PSLGTY++L
Sbjct: 495 RPTFAAKIFNLLPVEQKCTATFTAMISVYFSAGSADKALKIYKTMQKKGINPSLGTYDIL 554

Query: 541 LNGLAKNGRGVELNIYRREKKSFEISHHSRLNTILDDERICDLLF 584
           L GL ++GR  E++ YR+EKKS   + +   ++I  +E+ICDLLF
Sbjct: 555 LAGLERSGRICEIDTYRKEKKSLTANGY-YWDSIPMEEKICDLLF 591

BLAST of Cucsa.250610 vs. NCBI nr
Match: gi|645272910|ref|XP_008241621.1| (PREDICTED: pentatricopeptide repeat-containing protein At2g01390 [Prunus mume])

HSP 1 Score: 636.3 bits (1640), Expect = 5.2e-179
Identity = 322/563 (57.19%), Postives = 418/563 (74.25%), Query Frame = 1

Query: 29  SSKCLHSLHQYKRDKPISRFSRQS-RKGTKVAKKEE-VIPRLYTRDTVRNICNILRNCSW 88
           S K +HSL++ K+++P    +R+S +K  K +KK+E   P++Y R+T+ NI  IL+  +W
Sbjct: 29  SIKSIHSLNRIKQNEPSKPITRKSTKKHFKSSKKQEDADPKIYMRETIGNIYKILKYSTW 88

Query: 89  ASAQKHLEMLPIRWDSYLINQVLKTHPPLEKTWLFFNWASTLQVFKHDQYTYTTMLDIFG 148
            SAQ  L+ LPIRWDSY ++QVLKTHPP+EK WLFFNW + ++ FKHD +TYTTMLDIFG
Sbjct: 89  DSAQDQLQRLPIRWDSYTVSQVLKTHPPMEKAWLFFNWVAGIRGFKHDHFTYTTMLDIFG 148

Query: 149 EAGRISSMNYVFQQMKEKGIKIDAVTYTSLMHWRSNSGDVDGAIKVWKEMKANGCHPTVV 208
           EAGR+SSM +VF+QM+EK I+IDAVTYTSLMHW S++GDVDGA+KVW+EM+A GC PTVV
Sbjct: 149 EAGRVSSMTHVFKQMQEKDIRIDAVTYTSLMHWLSSAGDVDGAVKVWEEMRAQGCVPTVV 208

Query: 209 SYTAYIKILLDNGQINEATATYKKMLQSGLSPNCCTYTILMEYLIGEGKCKEALDIFSKM 268
           SYTAY+K+L ++ ++ EAT  YK+MLQSG SP C TYT+LMEYLIG GKCKEAL+IF KM
Sbjct: 209 SYTAYMKVLFNDNRVKEATDVYKEMLQSGCSPTCHTYTVLMEYLIGSGKCKEALEIFGKM 268

Query: 269 QDAGVYPDKAACNILIQKCCKSGERLVMTQILEFMKENRFVLRYPVFVEAHETLKSCSVS 328
           QDAGV PDKAACNILI+  CK GE   M Q+L FMKE+R  LRYPVF+EA  T K   VS
Sbjct: 269 QDAGVQPDKAACNILIENLCKVGETWTMNQVLWFMKEHRLALRYPVFLEAIRTFKIAGVS 328

Query: 329 YALLKQVNPHMEIESISKGEVVDVSTGSNTVPPNVDNELLAMLLKDNKLTAVDHMLIGIV 388
            +LL+QV+PH  IES    E  ++   +   P  +D  L+ +LLK   L A+DH+L G  
Sbjct: 329 DSLLRQVHPHFSIES-GNQETGELKATAADAPSTMDEWLVMILLKKENLVAIDHLLAGNE 388

Query: 389 DKNIQLDSSIIYSIIEVNCKSNRPNSALLAFDYCLKNSVNIKRKLYLDLIGILIRSSIYP 448
           DKNI+L+S+II +IIEVNC   RP+ ALLAF+Y +K  + ++R  YL LIG LIRS+ +P
Sbjct: 389 DKNIKLNSAIISTIIEVNCGLGRPDGALLAFEYSVKMGIIVERNAYLSLIGALIRSNSFP 448

Query: 449 KLLEIVQEMYTQGHCLGLYHATLILYSLGKAGKPQYARKVFNMLPEELKCTATYTALVDG 508
           K++EIV EM   G+  G Y + L++Y LG+A +   A KVFN+LP++ KCT TYTAL+  
Sbjct: 449 KVVEIVVEMIRAGYSPGTYLSALLIYRLGRARRTNCAAKVFNLLPDDHKCTTTYTALMGV 508

Query: 509 YFSAGSSGKGLKIFETMRKKGFTPSLGTYNVLLNGLAKNGRGVELNIYRREKKSFEISHH 568
           YFSAGS+ +GLKIF+ MR +GF P LGTYNVLL GL K GR  E  +YR+EKKS +    
Sbjct: 509 YFSAGSADRGLKIFDMMRGEGFLPFLGTYNVLLAGLQKLGRVRESEMYRKEKKSLQSDGQ 568

Query: 569 SRLNTILDDERICDLLF-GELVS 589
           S+ N +  D++ICD LF G++VS
Sbjct: 569 SQ-NAVPIDQKICDFLFAGDVVS 589

BLAST of Cucsa.250610 vs. NCBI nr
Match: gi|1009158915|ref|XP_015897536.1| (PREDICTED: pentatricopeptide repeat-containing protein At2g01390-like [Ziziphus jujuba])

HSP 1 Score: 634.8 bits (1636), Expect = 1.5e-178
Identity = 323/578 (55.88%), Postives = 420/578 (72.66%), Query Frame = 1

Query: 7   FSLLLSNYVVSSAIRKRIYQNISSKCLHSLHQYKRDKPISRF-SRQSRKGTKVAKKEEVI 66
           FS      +VS A         S K +HS   +K++K I  F  R+SRK  K   KE V 
Sbjct: 10  FSRNSQKILVSPATFSNSLVKNSIKFVHSFDHFKQNKSIKPFLGRKSRKYPKTIAKEAVD 69

Query: 67  PRLYTRDTVRNICNILRNCSWASAQKHLEMLPIRWDSYLINQVLKTHPPLEKTWLFFNWA 126
           P++Y RDT+  I  ILR  +W SAQ  L+ LP+RWDSY +NQ+LKTHPP+EK WLFFNWA
Sbjct: 70  PKVYMRDTIGKIYKILRYSTWDSAQDQLQGLPVRWDSYTVNQILKTHPPMEKAWLFFNWA 129

Query: 127 STLQVFKHDQYTYTTMLDIFGEAGRISSMNYVFQQMKEKGIKIDAVTYTSLMHWRSNSGD 186
             L+ FKHDQ+TYTTM+DIFGEAGRISSMN+VF+QM+E+ I+IDAVTYTSLMHW  +SGD
Sbjct: 130 DRLKGFKHDQFTYTTMIDIFGEAGRISSMNFVFKQMQEREIEIDAVTYTSLMHWIYSSGD 189

Query: 187 VDGAIKVWKEMKANGCHPTVVSYTAYIKILLDNGQINEATATYKKMLQSGLSPNCCTYTI 246
           VDGA++VW++MKANGC PTVVSYTAY+KIL ++ ++ +A   YK+MLQSG SPNC TYT+
Sbjct: 190 VDGAMEVWEQMKANGCKPTVVSYTAYMKILFNSNRVEKAIDAYKEMLQSGCSPNCHTYTV 249

Query: 247 LMEYLIGEGKCKEALDIFSKMQDAGVYPDKAACNILIQKCCKSGERLVMTQILEFMKENR 306
           LMEYLIG GKCKEAL+IF KMQ+AGV PDKAACNILI+K C+SG    MTQIL++MKEN 
Sbjct: 250 LMEYLIGSGKCKEALEIFDKMQEAGVQPDKAACNILIKKLCESGATWAMTQILQYMKENH 309

Query: 307 FVLRYPVFVEAHETLKSCSVSYALLKQVNPHMEIESISKGEVVDVSTGSNTVPPNVDNEL 366
            VLRYPVF+EA E LK    S +LL+QVNPH   E  S     D           +D  L
Sbjct: 310 IVLRYPVFLEACEILKVSGESDSLLRQVNPHFSNEPGSTDG--DFGKTPAAASFTLDEGL 369

Query: 367 LAMLLKDNKLTAVDHMLIGIVDKNIQLDSSIIYSIIEVNCKSNRPNSALLAFDYCLKNSV 426
           + +LL+   L AV+ +L  I++KNI+L+S+II +II+VNC   R + ALLAF+Y +K  +
Sbjct: 370 ILILLRKENLAAVNCLLAEIINKNIKLNSAIISTIIKVNCDHCRLHDALLAFEYSVKIGL 429

Query: 427 NIKRKLYLDLIGILIRSSIYPKLLEIVQEMYTQGHCLGLYHATLILYSLGKAGKPQYARK 486
            I+R  YL L+G L+R+  + K++++V+EM   GH LG+Y AT+++Y LG A +P +A +
Sbjct: 430 IIERNSYLALVGALVRAKSFTKVMDVVEEMIRAGHSLGIYAATILIYRLGCARRPIFAMR 489

Query: 487 VFNMLPEELKCTATYTALVDGYFSAGSSGKGLKIFETMRKKGFTPSLGTYNVLLNGLAKN 546
           +FN+ P++ KCTATYTAL+  YFSAG+  KGLKIFETM+ KG  PSLGTYNVLL+GL+K+
Sbjct: 490 IFNLFPDDHKCTATYTALIGAYFSAGAVDKGLKIFETMKGKGILPSLGTYNVLLSGLSKS 549

Query: 547 GRGVELNIYRREKKSFEISHHSRLNTILDDERICDLLF 584
           GR  E+ I+R+EKK  +I  H   + +  D++ICDLLF
Sbjct: 550 GRVNEVEIFRKEKKKLQIDGHPH-DIVPVDKKICDLLF 584