BLAST of Cucsa.250450 vs. Swiss-Prot
Match:
PME20_ARATH (Probable pectinesterase/pectinesterase inhibitor 20 OS=Arabidopsis thaliana GN=PME20 PE=2 SV=2)
HSP 1 Score: 465.3 bits (1196), Expect = 8.8e-130
Identity = 260/544 (47.79%), Postives = 331/544 (60.85%), Query Frame = 1
Query: 24 SAAPTADICQSTTSPPFCNSFLPKSPDSIHSHCRFTLHQALAHARTFLSLVNAQLN---- 83
+A ++IC+ P +C S LP P I+S+ R +L ++L+ AR F+S+++A+L+
Sbjct: 31 NATSPSNICRFAPDPSYCRSVLPNQPGDIYSYGRLSLRRSLSRARRFISMIDAELDRKGK 90
Query: 84 --LLPSLSALHDCRCLAEANLDFLFQTFSIVNSTTTTLPYYDAHEMLSLISAIITNVDTC 143
++ AL DC+ LA +D+L + +ST T L A ++ + +SA ITN TC
Sbjct: 91 VAAKSTVGALEDCKFLASLTMDYLLSSSQTADSTKT-LSLSRAEDVHTFLSAAITNEQTC 150
Query: 144 YEGLASLNSAVGLVDKVLEAISFDKKLYSLYLSLFKMGWV-----SKDLKAPTFPKMNHF 203
EGL S S GL + D KLY + L+LF GWV S+ + P F
Sbjct: 151 LEGLKSTASENGLSGDLFN----DTKLYGVSLALFSKGWVPRRQRSRPIWQPQARFKKFF 210
Query: 204 GAGKGQLKLKMSPKDRAYYERLVHRNKPPGARRLLQTNYQDDGIVVNGIVGVDQNGMYDF 263
G G+L LKM+ + RA Y + R +LLQ++ D + V+ IV V QNG +F
Sbjct: 211 GFRNGKLPLKMTERARAVYNTVTRR-------KLLQSDA--DAVQVSDIVTVIQNGTGNF 270
Query: 264 TNITAAIAAAPNKTTVAKGYFLIFVAAGIYNETVLVPKEKD------------------- 323
T I AAIAAAPNKT + GYFLI+V AG+Y E V VPK K
Sbjct: 271 TTINAAIAAAPNKTDGSNGYFLIYVTAGLYEEYVEVPKNKRYVMMIGDGINQTVITGNRS 330
Query: 324 -----------TFSVEGTGFLGVNLTITNTAGSAKHQAVALRVSADNVTLYNCIFEGYQD 383
TF + G F+GVN+TI NTAG K QAVALR D Y+C FE YQD
Sbjct: 331 VVDGWTTFNSATFILSGPNFIGVNITIRNTAGPTKGQAVALRSGGDLSVFYSCSFEAYQD 390
Query: 384 TLYTHSLRQFYRECDVYGTVDFIFGNAAVVLQNCNIYARLPMSGQFNALTAQGRTDPNQN 443
TLYTHSLRQFYRECDVYGTVDFIFGNAAVVLQNCN+Y R P GQ N +TAQGRTDPNQN
Sbjct: 391 TLYTHSLRQFYRECDVYGTVDFIFGNAAVVLQNCNLYPRQPRKGQSNEVTAQGRTDPNQN 450
Query: 444 TGTSIHNCTIKATPELAASPAT-KSYLGRPWKQYSRTVYMQSFIDSFIDPVGWKEWDGTL 503
TGT+IH CTI+ +LA S T K+YLGRPWK+YSRTV MQ++ID F++P GW W G
Sbjct: 451 TGTAIHGCTIRPADDLATSNYTVKTYLGRPWKEYSRTVVMQTYIDGFLEPSGWNAWSGDF 510
Query: 504 NLNTSYYAEFNNSGPGCDTSQRASW-AVGVINATVASNFTVSQLLAGDKWLPPTEVPYTG 525
L+T YYAE+NN+GPG DT+ R +W VINAT ASNFTV+ L G+ W+ T VP+ G
Sbjct: 511 ALSTLYYAEYNNTGPGSDTTNRVTWPGYHVINATDASNFTVTNFLVGEGWIGQTGVPFVG 560
BLAST of Cucsa.250450 vs. Swiss-Prot
Match:
PME7_ARATH (Probable pectinesterase/pectinesterase inhibitor 7 OS=Arabidopsis thaliana GN=PME7 PE=2 SV=1)
HSP 1 Score: 453.4 bits (1165), Expect = 3.5e-126
Identity = 263/581 (45.27%), Postives = 344/581 (59.21%), Query Frame = 1
Query: 5 NPISLLTFLTLLF--LLSPSSSAAPTADICQSTTSPPFCNSFLPKSPDSIHSHCRFTLHQ 64
+PI +L L+ +L+ S + + ++ IC++T P +C S P S ++ + F++ +
Sbjct: 3 SPIFILITLSFFLQSVLASSQTLSNSSTICKTTPDPKYCKSVFPHSQGNVQQYGCFSIRK 62
Query: 65 ALAHARTFLSLV------NAQLNLLPSLSALHDCRCLAEANLDFLFQTFSIVNSTTTT-- 124
+L+ +R F+ V NA L+ + AL DCR LA +D+L +F VN T+
Sbjct: 63 SLSQSRKFIRTVDRYIKRNAHLSQPAVIRALQDCRFLAGLTMDYLLTSFETVNDTSAKTS 122
Query: 125 ---LPYYDAHEMLSLISAIITNVDTCYEGL---ASLNSAVGLVDKVLEAISFDKKLYSLY 184
L + A ++ +L+SA +TN TC EGL AS ++ + V + D KL +
Sbjct: 123 FKPLSFPKADDIQTLLSAALTNEQTCLEGLTTAASYSATWTVRTGVALPLVNDTKLLGVS 182
Query: 185 LSLFKMGWVSKDLKAPTF----PKMNHFGAGK-------GQLKLKMSPKDRAYYERLVHR 244
L+LF GWV K K F P+ K G L LKM+ K +A YE L R
Sbjct: 183 LALFTKGWVPKKKKRAGFAWAQPRSGSSTHTKPFRLFRNGALPLKMTEKTKAVYESLSRR 242
Query: 245 NKPPGARRLLQTNYQDDG--IVVNGIVGVDQNGMYDFTNITAAIAAAPNKTTVAKGYFLI 304
G DDG ++++ IV V Q+G +FTNITAA+AAAPN T + G+FLI
Sbjct: 243 KLADGD----SNGDGDDGSMVLISDIVTVSQDGTGNFTNITAAVAAAPNNTDGSAGFFLI 302
Query: 305 FVAAGIYNETVLVPKEKD------------------------------TFSVEGTGFLGV 364
+V AGIY E + + K K TF+V F+ V
Sbjct: 303 YVTAGIYEEYISIAKNKRYMMMIGDGINQTVVTGNRSVVDGWTTFNSATFAVTAPNFVAV 362
Query: 365 NLTITNTAGSAKHQAVALRVSADNVTLYNCIFEGYQDTLYTHSLRQFYRECDVYGTVDFI 424
N+T NTAG KHQAVALR AD Y+C FE YQDTLYTHSLRQFYRECDVYGTVDFI
Sbjct: 363 NITFRNTAGPEKHQAVALRSGADFSIFYSCSFEAYQDTLYTHSLRQFYRECDVYGTVDFI 422
Query: 425 FGNAAVVLQNCNIYARLPMSGQFNALTAQGRTDPNQNTGTSIHNCTIKATPELAASPAT- 484
FGNAAVV QNCN+Y R PM QFNA+TAQGR+DPNQNTGTSI NCTIK +L +S T
Sbjct: 423 FGNAAVVFQNCNLYPRKPMPNQFNAITAQGRSDPNQNTGTSIQNCTIKPADDLVSSNYTV 482
Query: 485 KSYLGRPWKQYSRTVYMQSFIDSFIDPVGWKEWDGTLNLNTSYYAEFNNSGPGCDTSQRA 525
K+YLGRPWK+YSRTVYMQS+ID F++PVGW+EW+G L+T YYAE+NN+GPG +T+ R
Sbjct: 483 KTYLGRPWKEYSRTVYMQSYIDGFVEPVGWREWNGDFALSTLYYAEYNNTGPGSNTTNRV 542
BLAST of Cucsa.250450 vs. Swiss-Prot
Match:
PME41_ARATH (Probable pectinesterase/pectinesterase inhibitor 41 OS=Arabidopsis thaliana GN=PME41 PE=2 SV=2)
HSP 1 Score: 448.7 bits (1153), Expect = 8.5e-125
Identity = 263/582 (45.19%), Postives = 342/582 (58.76%), Query Frame = 1
Query: 7 ISLLTFLTLLFL-----------LSPSSSAAPTADICQSTTSPPFCNSFLPK-SPDSIHS 66
+SL FL LFL L PS+S++ IC++T P FC S P+ S +
Sbjct: 2 LSLKLFLVTLFLSLQTLFIASQTLLPSNSSST---ICKTTPDPKFCKSVFPQTSQGDVRE 61
Query: 67 HCRFTLHQALAHARTFLSLVNAQL---NLLPSLSA---LHDCRCLAEANLDFLFQTFSIV 126
+ RF+L ++L +R F ++ L N L S SA L DCR LA D+L +F V
Sbjct: 62 YGRFSLRKSLTQSRKFTRTIDRYLKRNNALLSQSAVGALQDCRYLASLTTDYLITSFETV 121
Query: 127 NSTTT--TLPYYDAHEMLSLISAIITNVDTCYEGL-ASLNSAVGLVDKVLEAISFDKKLY 186
N TT+ TL + A E+ +L+SA +TN TC +G+ + +S+ + + V + D KL+
Sbjct: 122 NITTSSKTLSFSKADEIQTLLSAALTNEQTCLDGINTAASSSWTIRNGVALPLINDTKLF 181
Query: 187 SLYLSLFKMGWVSKDLKAPT-----FPKMNHFGAG------KGQLKLKMSPKDRAYYERL 246
S+ L+LF GWV K K PK H G L LKM+ RA YE L
Sbjct: 182 SVSLALFTKGWVPKKKKQVASYSWAHPKNTHSHTKPFRHFRNGALPLKMTEHTRAVYESL 241
Query: 247 VHRNKPPGARRLLQTNYQDDGIVVNGIVGVDQNGMYDFTNITAAIAAAPNKTTVAKGYFL 306
R+L + + ++V+ IV V+QNG +FT IT A+ +APNKT GYF+
Sbjct: 242 -------SRRKLADDDNDVNTVLVSDIVTVNQNGTGNFTTITEAVNSAPNKTDGTAGYFV 301
Query: 307 IFVAAGIYNETVLVPKEKD------------------------------TFSVEGTGFLG 366
I+V +G+Y E V++ K K TF+V F+
Sbjct: 302 IYVTSGVYEENVVIAKNKRYLMMIGDGINRTVVTGNRNVVDGWTTFNSATFAVTSPNFVA 361
Query: 367 VNLTITNTAGSAKHQAVALRVSADNVTLYNCIFEGYQDTLYTHSLRQFYRECDVYGTVDF 426
VN+T NTAG KHQAVA+R SAD Y+C FE YQDTLYTHSLRQFYRECD+YGTVDF
Sbjct: 362 VNMTFRNTAGPEKHQAVAMRSSADLSIFYSCSFEAYQDTLYTHSLRQFYRECDIYGTVDF 421
Query: 427 IFGNAAVVLQNCNIYARLPMSGQFNALTAQGRTDPNQNTGTSIHNCTIKATPELAASPAT 486
IFGNAAVV Q+CN+Y R PM QFNA+TAQGRTDPNQNTG SIHNCTIK +L +S T
Sbjct: 422 IFGNAAVVFQDCNLYPRQPMQNQFNAITAQGRTDPNQNTGISIHNCTIKPADDLVSSNYT 481
Query: 487 -KSYLGRPWKQYSRTVYMQSFIDSFIDPVGWKEWDGTLNLNTSYYAEFNNSGPGCDTSQR 525
K+YLGRPWK+YSRTV+MQS+ID ++PVGW+EW+G L+T YYAE+NN+G G T+ R
Sbjct: 482 VKTYLGRPWKEYSRTVFMQSYIDEVVEPVGWREWNGDFALSTLYYAEYNNTGSGSSTTDR 541
BLAST of Cucsa.250450 vs. Swiss-Prot
Match:
PME25_ARATH (Probable pectinesterase/pectinesterase inhibitor 25 OS=Arabidopsis thaliana GN=PME25 PE=2 SV=1)
HSP 1 Score: 373.6 bits (958), Expect = 3.5e-102
Identity = 220/552 (39.86%), Postives = 301/552 (54.53%), Query Frame = 1
Query: 19 LSPSSSAAPTADICQSTTSPPFCNSFL---PKSPDSIHSHCRFTLHQALAHARTFLSLVN 78
L PS S +P+ C+ST P C + L SP + + +FT+ Q L A ++
Sbjct: 71 LPPSQSQSPSL-ACKSTPYPKLCRTILNAVKSSPSDPYRYGKFTIKQCLKQASRLSKVIT 130
Query: 79 AQLNLLPS---------LSALHDCRCLAEANLDFLFQTFSIVNSTTTTLPYYDAHEMLSL 138
+ + S + A+ DC L+E ++++L +T + T + + SL
Sbjct: 131 SYARRVESKPGSATAEEIGAVADCGELSELSVNYL-ETVTTELKTAQVMTAALVEHVNSL 190
Query: 139 ISAIITNVDTCYEGLASLNSAVGLVDKVLEAISFDKKLYSLYLSLFKMGWVSKDLKAPTF 198
+S ++TN TC +GL S G + + +LYS+ L L ++++LK
Sbjct: 191 LSGVVTNQQTCLDGLVEAKS--GFAAAIGSPMGNLTRLYSISLGLVSHA-LNRNLKRFKA 250
Query: 199 PKMNHFGAGKGQLKLKMSPKDRAYYERLVHRNKPPGARRLLQTNYQDDG--IVVNGIVGV 258
K G G + + + + + R L + G I+V+ V V
Sbjct: 251 SKGKILGGGNSTYREPLETLIKGLRKTCDNDKDCRKTSRNLGELGETSGGSILVSKAVIV 310
Query: 259 DQNGMYDFTNITAAIAAAPNKTTVAKGYFLIFVAAGIYNETVLVPKEK------------ 318
+FT IT AIAAAPN T GYF+I+ G+Y E ++VP K
Sbjct: 311 GPFKSDNFTTITDAIAAAPNNTRPEDGYFVIYAREGVYEEYIVVPINKKNLMLMGDGINK 370
Query: 319 ------------------DTFSVEGTGFLGVNLTITNTAGSAKHQAVALRVSADNVTLYN 378
+F+V G F+ V++T NTAG KHQAVALR +A+ + Y
Sbjct: 371 TIITGNHNVVDGWTTYNCSSFAVVGERFMAVDVTFRNTAGPEKHQAVALRNNAEGSSFYR 430
Query: 379 CIFEGYQDTLYTHSLRQFYRECDVYGTVDFIFGNAAVVLQNCNIYARLPMSGQFNALTAQ 438
C FEGYQDTLY HSLRQFYRECD+YGTVDFIFGNAA + QNCNIYAR PM+ Q NA+TA
Sbjct: 431 CSFEGYQDTLYVHSLRQFYRECDIYGTVDFIFGNAAAIFQNCNIYARKPMAKQKNAITAH 490
Query: 439 GRTDPNQNTGTSIHNCTIKATPELAASP-ATKSYLGRPWKQYSRTVYMQSFIDSFIDPVG 498
GR DPNQNTG SI NCTIKA P+LAA P + ++LGRPWK YSRTV+MQS+I + PVG
Sbjct: 491 GRLDPNQNTGISIINCTIKAAPDLAAEPKSAMTFLGRPWKPYSRTVFMQSYISDIVQPVG 550
Query: 499 WKEWDGTLNLNTSYYAEFNNSGPGCDTSQRASW-AVGVINATVASNFTVSQLLAGDKWLP 525
W EW+GT+ L+T YY E++N GPG +T+QR W ++N A NFTV GD WLP
Sbjct: 551 WLEWNGTIGLDTIYYGEYSNFGPGANTNQRVQWLGYNLLNLAEAMNFTVYNFTMGDTWLP 610
BLAST of Cucsa.250450 vs. Swiss-Prot
Match:
PME47_ARATH (Probable pectinesterase/pectinesterase inhibitor 47 OS=Arabidopsis thaliana GN=PME47 PE=2 SV=1)
HSP 1 Score: 369.0 bits (946), Expect = 8.6e-101
Identity = 222/551 (40.29%), Postives = 299/551 (54.26%), Query Frame = 1
Query: 20 SPSS-SAAPTADICQSTTSPPFCNSFLP---KSPDSIHSHCRFTLHQALAHARTFLSLVN 79
SPS S P C+ST P C + L SP + + +FT+ Q L AR ++N
Sbjct: 75 SPSQPSPLPPNIACKSTPYPKLCRTILSAVKSSPSDPYHYGKFTMKQCLKQARRLSKVIN 134
Query: 80 --AQ-------LNLLPSLSALHDCRCLAEANLDFLFQTFSIVNSTTTTLPYYDAHEMLSL 139
AQ + + +SA+ DC LAE ++++L +T + + + SL
Sbjct: 135 RFAQRVEADPGTSTVEEVSAVADCGELAELSVEYL-ETVTEELKAAELMTAALVDRVTSL 194
Query: 140 ISAIITNVDTCYEGLASLNSAVGLVDKVLEAISFDKKLYSLYLSLFKMGWVSKDLKAPTF 199
+ ++TN TC +GL S G + + +LYS+ L L ++++LK
Sbjct: 195 LGGVVTNQQTCLDGLVDAKS--GFATAIGTPLGNLTRLYSVSLGLVSHA-LNRNLKRYKG 254
Query: 200 PKMNHFGAGKGQLKLKMSPKDRAYYERLVHRNKPPGARRLLQTNYQDDG--IVVNGIVGV 259
K FG G ++ + + + A R L + G I+V V V
Sbjct: 255 SKGKIFGGGNKPVREPLETLIKVLRKTCDKGKDCRKANRNLGELGETSGGSILVREAVTV 314
Query: 260 DQNGMYDFTNITAAIAAAPNKTTVAKGYFLIFVAAGIYNETVLV---------------- 319
+F IT A+AAAPN T +GYF+I+ AG+Y E V++
Sbjct: 315 GPYETDNFPTITEAVAAAPNHTFPEQGYFVIYARAGLYEEYVVISNKKRNIMLIGDGINK 374
Query: 320 --------------PKEKDTFSVEGTGFLGVNLTITNTAGSAKHQAVALRVSADNVTLYN 379
TF+V G F+ V++T NTAG KHQAVA+R +AD T Y
Sbjct: 375 TIISGNHSFIDGWTTYNSSTFAVVGDRFVAVDVTFRNTAGPEKHQAVAVRNNADGSTFYR 434
Query: 380 CIFEGYQDTLYTHSLRQFYRECDVYGTVDFIFGNAAVVLQNCNIYARLPMSGQFNALTAQ 439
C FEGYQDTLY HSLRQFYRECD+YGT+DFIFGNAA + QNCNIYAR PM+ Q NA+TA
Sbjct: 435 CSFEGYQDTLYVHSLRQFYRECDIYGTIDFIFGNAAAIFQNCNIYARKPMANQKNAVTAH 494
Query: 440 GRTDPNQNTGTSIHNCTIKATPELAASP-ATKSYLGRPWKQYSRTVYMQSFIDSFIDPVG 499
GRTDPNQ TG SI NCTI A P+LAA P +T ++LGRPWK YSRTVY+QS+I + PVG
Sbjct: 495 GRTDPNQKTGISIINCTIGAAPDLAADPKSTMTFLGRPWKPYSRTVYIQSYISDVVQPVG 554
Query: 500 WKEWDGTLNLNTSYYAEFNNSGPGCDTSQRASWA-VGVINATVASNFTVSQLLAGDKWLP 524
W EW+GT L+T Y E++N GPG DTS+R W+ ++N A NFTV GD WLP
Sbjct: 555 WLEWNGTTGLDTISYGEYDNFGPGADTSKRVQWSGYSLLNLVQAMNFTVYNFTLGDTWLP 614
BLAST of Cucsa.250450 vs. TrEMBL
Match:
A0A0A0LJX5_CUCSA (Pectinesterase OS=Cucumis sativus GN=Csa_2G296080 PE=4 SV=1)
HSP 1 Score: 585.5 bits (1508), Expect = 6.5e-164
Identity = 302/352 (85.80%), Postives = 312/352 (88.64%), Query Frame = 1
Query: 1 MVSSNPISLLTFLTLLFLLSPSSSAAPTADICQSTTSPPFCNSFLPKSPDSIHSHCRFTL 60
MVSSNPISLLTFLTLLFLLSPSSSAAPTADICQSTTSPPFCNSFLPKSPDSIHSHCRFTL
Sbjct: 1 MVSSNPISLLTFLTLLFLLSPSSSAAPTADICQSTTSPPFCNSFLPKSPDSIHSHCRFTL 60
Query: 61 HQALAHARTFLSLVNAQLNLLPSLSALHDCRCLAEANLDFLFQTFSIVNSTTTTLPYYDA 120
HQALAHARTFLSLVNAQLNLLPSLSALHDCRCLAEANLDFLFQTFSIVNSTTTTLPYYDA
Sbjct: 61 HQALAHARTFLSLVNAQLNLLPSLSALHDCRCLAEANLDFLFQTFSIVNSTTTTLPYYDA 120
Query: 121 HEMLSLISAIITNVDTCYEGLASLNSAVGLVDKVLEAISFDKKLYSLYLSLFKMGWVSKD 180
HEMLSLISAIITNVDTCYEGLASLNSAVGLVDKVLEAISFDKKLYSLYLSLFKMGWVSKD
Sbjct: 121 HEMLSLISAIITNVDTCYEGLASLNSAVGLVDKVLEAISFDKKLYSLYLSLFKMGWVSKD 180
Query: 181 LKAPTFPKMNHFGAGKGQLKLKMSPKDRAYYERLVHRNKPPGARRLLQTNYQDDGIVVNG 240
LKAPTFPKMNHFGAGKGQLKLKMSPKDRAYYERLVHRNKPPGARRLLQTNYQDDGIVVNG
Sbjct: 181 LKAPTFPKMNHFGAGKGQLKLKMSPKDRAYYERLVHRNKPPGARRLLQTNYQDDGIVVNG 240
Query: 241 IVGVDQNGMYDFTNITAAIAAAPNKTTVAKGYFLIFVAAGIYNETVLVPKEKDTFSVEGT 300
IVGVDQNGMYDFTNITAAIAAAPNKTTVAKGYFLIFVAAGIYNETVLVPKEK + G
Sbjct: 241 IVGVDQNGMYDFTNITAAIAAAPNKTTVAKGYFLIFVAAGIYNETVLVPKEKRYVLLIGE 300
Query: 301 G----FLGVNLTITNTAGSAKHQAVALRVSADNVTLYNCIFEGYQDTLYTHS 349
G + N + + + + V L S + ++ Y T+Y S
Sbjct: 301 GNNQTIITGNKNVVDGSTTFNSATVELAASPATKSYLGRPWKQYSRTVYMQS 352
BLAST of Cucsa.250450 vs. TrEMBL
Match:
A0A0A0LJX5_CUCSA (Pectinesterase OS=Cucumis sativus GN=Csa_2G296080 PE=4 SV=1)
HSP 1 Score: 232.3 bits (591), Expect = 1.4e-57
Identity = 110/111 (99.10%), Postives = 110/111 (99.10%), Query Frame = 1
Query: 414 ATPELAASPATKSYLGRPWKQYSRTVYMQSFIDSFIDPVGWKEWDGTLNLNTSYYAEFNN 473
AT ELAASPATKSYLGRPWKQYSRTVYMQSFIDSFIDPVGWKEWDGTLNLNTSYYAEFNN
Sbjct: 323 ATVELAASPATKSYLGRPWKQYSRTVYMQSFIDSFIDPVGWKEWDGTLNLNTSYYAEFNN 382
Query: 474 SGPGCDTSQRASWAVGVINATVASNFTVSQLLAGDKWLPPTEVPYTGGLIS 525
SGPGCDTSQRASWAVGVINATVASNFTVSQLLAGDKWLPPTEVPYTGGLIS
Sbjct: 383 SGPGCDTSQRASWAVGVINATVASNFTVSQLLAGDKWLPPTEVPYTGGLIS 433
HSP 2 Score: 526.6 bits (1355), Expect = 3.6e-146
Identity = 286/560 (51.07%), Postives = 362/560 (64.64%), Query Frame = 1
Query: 7 ISLLTFLTLLFLLS---PSSSAAPTADICQSTTSPPFCNSFLPKSPDSIHSHCRFTLHQA 66
+ +L FL L L+ P++S +P IC+ST P +CNS LP +++ + RF++ ++
Sbjct: 9 VIILIFLPSLLALADITPNTSVSP-GTICKSTPDPSYCNSVLPPQNGNVYEYGRFSVRKS 68
Query: 67 LAHARTFLSLVNAQLNL-------LPSLSALHDCRCLAEANLDFLFQTFSIVNSTTTTLP 126
L+ A FL+LVN L L P++ AL DC+ LAE N+DFL + VN TT LP
Sbjct: 69 LSQATNFLNLVNRYLQLQRRSSLSTPAIHALEDCQSLAELNIDFLSSSLETVNRTTKFLP 128
Query: 127 YYDAHEMLSLISAIITNVDTCYEGLASLNSAVGLVDKVLEAISFDKKLYSLYLSLFKMGW 186
A ++ +L+SAI+TN TC EGL + SA L + + +S D KLYS+ L+LF GW
Sbjct: 129 TSQADDIQTLLSAILTNQQTCLEGLQATASAWRLKNGLSVPLSNDTKLYSVSLALFTKGW 188
Query: 187 VSKDLKAPTF-PKMNHFGAGKGQLKLKMSPKDRAYYERLVHRNKPPGARRLLQTNYQDDG 246
V ++ F P H G G+L LKMS + RA YE + R+LLQ D+
Sbjct: 189 VPENANVTAFQPSAKHRGFRNGRLPLKMSSRTRAIYESV-------SRRKLLQATVGDE- 248
Query: 247 IVVNGIVGVDQNGMYDFTNITAAIAAAPNKTTVAKGYFLIFVAAGIYNETVLVPKEKD-- 306
+ V IV V ++G +FT I+ A+AAAPNKT+ GYFLI+V AG+Y E V + K+K
Sbjct: 249 VKVKDIVTVSKDGNGNFTTISDAVAAAPNKTSSTAGYFLIYVTAGVYEENVSIDKKKTYL 308
Query: 307 ----------------------------TFSVEGTGFLGVNLTITNTAGSAKHQAVALRV 366
TF+V G F+GVN+TI NTAG+ KHQAVALR
Sbjct: 309 MMVGDGINKTIITGNRSVVDGWTTFKSATFAVVGARFVGVNMTIRNTAGAEKHQAVALRN 368
Query: 367 SADNVTLYNCIFEGYQDTLYTHSLRQFYRECDVYGTVDFIFGNAAVVLQNCNIYARLPMS 426
AD T Y+C FEGYQDTLYTHSLRQFYRECD+YGTVDFIFGNAAVV QNCN+Y RLPMS
Sbjct: 369 GADLSTFYSCSFEGYQDTLYTHSLRQFYRECDIYGTVDFIFGNAAVVFQNCNLYPRLPMS 428
Query: 427 GQFNALTAQGRTDPNQNTGTSIHNCTIKATPELAAS-PATKSYLGRPWKQYSRTVYMQSF 486
GQFN++TAQGRTDPNQNTGTSIHNCTI+ +LAA+ A ++YLGRPWK YSRTVYMQSF
Sbjct: 429 GQFNSITAQGRTDPNQNTGTSIHNCTIRPADDLAANIDAAETYLGRPWKNYSRTVYMQSF 488
Query: 487 IDSFIDPVGWKEWDGTLNLNTSYYAEFNNSGPGCDTSQRASW-AVGVINATVASNFTVSQ 524
+D+ I+ GW+EWDG L+T YYAEFNN+GPG T+ R +W VINATVA+NFTV+
Sbjct: 489 MDTVINSAGWREWDGDFALSTLYYAEFNNTGPGSTTANRVTWPGYHVINATVAANFTVAN 548
BLAST of Cucsa.250450 vs. TrEMBL
Match:
B9SAU3_RICCO (Pectinesterase OS=Ricinus communis GN=RCOM_1179060 PE=4 SV=1)
HSP 1 Score: 524.6 bits (1350), Expect = 1.4e-145
Identity = 284/565 (50.27%), Postives = 361/565 (63.89%), Query Frame = 1
Query: 8 SLLTFLTLLFLL-----------SPSSSAAPTADICQSTTSPPFCNSFLPKSPDSIHSHC 67
SL T L+LL LL SP S+ +C ST P +C S LPK +++ +
Sbjct: 9 SLTTLLSLLLLLLPFFTSLSLADSPPSNPTSPGSLCNSTPEPAYCKSVLPKHNANVYDYG 68
Query: 68 RFTLHQALAHARTFLSLVNAQLNLLPSLS-----ALHDCRCLAEANLDFLFQTFSIVNST 127
R+++ ++L+ +R FLSLV+ L SLS AL DCR LA N+DFL +F VN++
Sbjct: 69 RYSVRKSLSQSRKFLSLVDKYLARRSSLSISAIRALEDCRLLAGLNMDFLLNSFHTVNTS 128
Query: 128 TTTLPYYDAHEMLSLISAIITNVDTCYEGLASLNSAVGLVDKVLEAISFDKKLYSLYLSL 187
+TTL A ++ + +SAI+TN TC EGL + SA + + + +S D KLYS+ L+L
Sbjct: 129 STTLSSLKADDVQTFLSAILTNQQTCLEGLQATASAWSVKNGLAVPLSNDTKLYSVSLAL 188
Query: 188 FKMGWVSKDLKAPTF-PKMNHFGAGKGQLKLKMSPKDRAYYERLVHRNKPPGARRLLQTN 247
F GWV K K T+ P G+L L+MS K R +E + R+LLQT+
Sbjct: 189 FTKGWVPKKKKGRTWQPTGKQLAFSNGRLPLRMSSKTRTVFESV-------SRRKLLQTD 248
Query: 248 YQDDGIVVNGIVGVDQNGMYDFTNITAAIAAAPNKTTVAKGYFLIFVAAGIYNETVLVPK 307
Q+D ++V+ IV V+QNG DFT I A+AAAPN T + GYF+IFV AG+Y E V +PK
Sbjct: 249 DQNDAVLVSDIVTVNQNGTGDFTTINDAVAAAPNNTDGSNGYFMIFVTAGVYEEYVSIPK 308
Query: 308 EKD------------------------------TFSVEGTGFLGVNLTITNTAGSAKHQA 367
K TF+V ++GVN+T NTAG+ KHQA
Sbjct: 309 NKKYLMMVGAGINQTIITGNRSVVDGWTTFNSATFAVVAPNYVGVNITFRNTAGAIKHQA 368
Query: 368 VALRVSADNVTLYNCIFEGYQDTLYTHSLRQFYRECDVYGTVDFIFGNAAVVLQNCNIYA 427
VALR AD T Y+C FEGYQDTLYTHSLRQFY ECD+YGTVDFIFGNAAVV QNCN+Y
Sbjct: 369 VALRSGADLSTFYSCSFEGYQDTLYTHSLRQFYSECDIYGTVDFIFGNAAVVFQNCNLYP 428
Query: 428 RLPMSGQFNALTAQGRTDPNQNTGTSIHNCTIKATPELAASPAT-KSYLGRPWKQYSRTV 487
RLPMSGQFNA+TAQGRTDPNQNTGTSIHNCTI+A +LA+S +T ++YLGRPWK+YSRTV
Sbjct: 429 RLPMSGQFNAITAQGRTDPNQNTGTSIHNCTIRAADDLASSNSTVRTYLGRPWKEYSRTV 488
Query: 488 YMQSFIDSFIDPVGWKEWDGTLNLNTSYYAEFNNSGPGCDTSQRASW-AVGVINATVASN 524
YMQS++DS I P GW+ W G L+T YYAE+NN+GPG DT+ R +W VINAT A+N
Sbjct: 489 YMQSYMDSLIHPAGWQIWSGDFVLSTLYYAEYNNTGPGSDTNNRVTWEGYHVINATDAAN 548
BLAST of Cucsa.250450 vs. TrEMBL
Match:
M5VIT6_PRUPE (Pectinesterase OS=Prunus persica GN=PRUPE_ppa003639mg PE=4 SV=1)
HSP 1 Score: 524.2 bits (1349), Expect = 1.8e-145
Identity = 284/552 (51.45%), Postives = 357/552 (64.67%), Query Frame = 1
Query: 12 FLTLLFLLSPSSSAAPTADICQSTTSPPFCNSFLP--KSPDSIHSHCRFTLHQALAHART 71
F +L SSS IC+ST P FC S LP ++ ++++ + RF++ ++L+ +R
Sbjct: 18 FSSLSLAADNSSSPVSAGTICKSTPDPSFCKSVLPHNQTAENVYYYGRFSVRKSLSQSRK 77
Query: 72 FLSLVNAQLNLLPSLS-----ALHDCRCLAEANLDFLFQTFSIVNSTTTTLPYYDAHEML 131
FL+LV+ L SLS AL DCR LA N+DFL +F VN T+ TL A ++
Sbjct: 78 FLNLVDKYLKRRSSLSLTAVRALEDCRLLAGLNMDFLLNSFETVNKTSKTLSNLKADDVQ 137
Query: 132 SLISAIITNVDTCYEGLASLNSAVGLVDKVLEAISFDKKLYSLYLSLFKMGWVSKDLKAP 191
+ +SAI+TN TC +G+ S SA L K + D K YS+ L+LF GWV K K+
Sbjct: 138 TSLSAILTNQQTCLDGIQSTASAWSL--KTGLSAPNDTKFYSVSLALFTKGWVPKKKKSA 197
Query: 192 TF-PKMNHFGAGKGQLKLKMSPKDRAYYERLVHRNKPPGARRLLQTNYQDDGIVVNGIVG 251
T+ P G G+L LKMS + RA YE + R +LLQT +DDG++V+ +V
Sbjct: 198 TWKPTRKQRGFKNGRLPLKMSSRTRAVYEGVTKR-------KLLQTETEDDGVLVSEVVT 257
Query: 252 VDQNGMYDFTNITAAIAAAPNKTTVAKGYFLIFVAAGIYNETVLVPKEKD---------- 311
V QNG +F+ I AIAAAPN + GYFLI+V AG+Y E V + K K
Sbjct: 258 VSQNGTGNFSTINDAIAAAPNNSASTNGYFLIYVTAGVYEEYVSIAKNKRYLMMVGDGIN 317
Query: 312 --------------------TFSVEGTGFLGVNLTITNTAGSAKHQAVALRVSADNVTLY 371
TF+V GF+ VN+T NTAG+ KHQAVA+R AD T Y
Sbjct: 318 QTILTGNRSVVDGWTTFNSATFAVTAPGFVAVNMTFRNTAGAIKHQAVAVRNGADLSTFY 377
Query: 372 NCIFEGYQDTLYTHSLRQFYRECDVYGTVDFIFGNAAVVLQNCNIYARLPMSGQFNALTA 431
+C FE YQDTLYTHSLRQFYRECD+YGTVDFIFGNAAVV QNCN+Y RLPMSGQFNA+TA
Sbjct: 378 SCSFEAYQDTLYTHSLRQFYRECDIYGTVDFIFGNAAVVFQNCNLYPRLPMSGQFNAITA 437
Query: 432 QGRTDPNQNTGTSIHNCTIKATPELAAS-PATKSYLGRPWKQYSRTVYMQSFIDSFIDPV 491
QGRTDPNQNTGTSIHNCTI+A +LA+S +TK+YLGRPWK+YSRTVYMQS+IDS +DP
Sbjct: 438 QGRTDPNQNTGTSIHNCTIRAADDLASSNSSTKTYLGRPWKEYSRTVYMQSYIDSVVDPA 497
Query: 492 GWKEWDGTLNLNTSYYAEFNNSGPGCDTSQRASW-AVGVINATVASNFTVSQLLAGDKWL 524
GW+ WDG L+T YYAE+NN+GPG +T+ R +W VINAT A+NFTV+ L GD WL
Sbjct: 498 GWRAWDGEFALSTLYYAEYNNTGPGSNTTSRVTWPGYHVINATDAANFTVTNFLLGDDWL 557
BLAST of Cucsa.250450 vs. TrEMBL
Match:
A0A0L9VHI5_PHAAN (Pectinesterase OS=Phaseolus angularis GN=LR48_Vigan10g028400 PE=4 SV=1)
HSP 1 Score: 520.4 bits (1339), Expect = 2.6e-144
Identity = 281/553 (50.81%), Postives = 356/553 (64.38%), Query Frame = 1
Query: 10 LTFLTLLFLLSPSSSAAPTADICQSTTSPPFCNSFLPKSPDSIHSHCRFTLHQALAHART 69
L F++L+F +P +++ IC+ST P +C+S LP +++ + RF++ ++L+ A
Sbjct: 22 LLFISLVFAETPPTTSVSPVTICKSTPDPSYCSSVLPPRNANVYDYGRFSVKKSLSQATK 81
Query: 70 FLSLVNAQLNLLPSLS-----ALHDCRCLAEANLDFLFQTFSIVNSTTTTLPYYDAHEML 129
FL+LVN L SLS AL DC+ LAE N+DFL +F VN TTT LP A ++
Sbjct: 82 FLNLVNRYLQRRSSLSTSAIHALQDCQTLAELNVDFLSSSFQTVNRTTTLLPTSRADDIQ 141
Query: 130 SLISAIITNVDTCYEGLASLNSAVGLVDKVLEAISFDKKLYSLYLSLFKMGWVSKDLKAP 189
+L+SAI+TN TC EGL + SA + + + +S D KLY++ L+LF GWV +
Sbjct: 142 TLLSAILTNQQTCLEGLQATASAWRVSNGLFLPLSNDTKLYTVSLALFTKGWVPTNANVT 201
Query: 190 TF-PKMNHFGAGKGQLKLKMSPKDRAYYERLVHRNKPPGARRLLQTNYQDDGIVVNGIVG 249
F P H G+L LKMS + RA Y + R+LLQ N D+ + V IV
Sbjct: 202 AFQPPAKHRAFRNGRLPLKMSSRTRAIYNSV-------SRRKLLQGNVGDE-VKVKDIVT 261
Query: 250 VDQNGMYDFTNITAAIAAAPNKTTVAKGYFLIFVAAGIYNETVLVPKEKD---------- 309
V ++G +FT + AIAAAPNK++ GYFLI+V AG+Y E V + K+K
Sbjct: 262 VSKDGSGNFTTLNDAIAAAPNKSSSTSGYFLIYVTAGVYEENVSIDKKKTYLMMVGDGIN 321
Query: 310 --------------------TFSVEGTGFLGVNLTITNTAGSAKHQAVALRVSADNVTLY 369
TF+V G GF+GVN+TI NTAG+ KHQAVALR AD T Y
Sbjct: 322 KTIITGNRSVVDGWTTFKSATFAVVGAGFVGVNMTIRNTAGAEKHQAVALRNGADLSTFY 381
Query: 370 NCIFEGYQDTLYTHSLRQFYRECDVYGTVDFIFGNAAVVLQNCNIYARLPMSGQFNALTA 429
+C FEGYQDTLY HSLRQFYRECD+YGTVDFIFGNAA VLQNCN+Y RLPM+GQFNA+TA
Sbjct: 382 SCSFEGYQDTLYAHSLRQFYRECDIYGTVDFIFGNAATVLQNCNLYPRLPMNGQFNAITA 441
Query: 430 QGRTDPNQNTGTSIHNCTIKATPELAAS-PATKSYLGRPWKQYSRTVYMQSFIDSFIDPV 489
QGRTDPNQNTG I NCTI+A +LAA+ A ++YLGRPWK YSRTVYMQSF+DS IDP
Sbjct: 442 QGRTDPNQNTGICIQNCTIRAADDLAANINAAETYLGRPWKNYSRTVYMQSFMDSVIDPG 501
Query: 490 GWKEWDGTLNLNTSYYAEFNNSGPGCDTSQRASW-AVGVINATVASNFTVSQLLAGDKWL 525
GW+ WDG LNT YYAEFNN+GPG T+ R +W VINAT A+NFTVS L GD WL
Sbjct: 502 GWRAWDGDFALNTLYYAEFNNTGPGSTTANRVTWPGYHVINATDAANFTVSNFLLGDNWL 561
BLAST of Cucsa.250450 vs. TAIR10
Match:
AT2G47550.1 (AT2G47550.1 Plant invertase/pectin methylesterase inhibitor superfamily)
HSP 1 Score: 465.3 bits (1196), Expect = 4.9e-131
Identity = 260/544 (47.79%), Postives = 331/544 (60.85%), Query Frame = 1
Query: 24 SAAPTADICQSTTSPPFCNSFLPKSPDSIHSHCRFTLHQALAHARTFLSLVNAQLN---- 83
+A ++IC+ P +C S LP P I+S+ R +L ++L+ AR F+S+++A+L+
Sbjct: 31 NATSPSNICRFAPDPSYCRSVLPNQPGDIYSYGRLSLRRSLSRARRFISMIDAELDRKGK 90
Query: 84 --LLPSLSALHDCRCLAEANLDFLFQTFSIVNSTTTTLPYYDAHEMLSLISAIITNVDTC 143
++ AL DC+ LA +D+L + +ST T L A ++ + +SA ITN TC
Sbjct: 91 VAAKSTVGALEDCKFLASLTMDYLLSSSQTADSTKT-LSLSRAEDVHTFLSAAITNEQTC 150
Query: 144 YEGLASLNSAVGLVDKVLEAISFDKKLYSLYLSLFKMGWV-----SKDLKAPTFPKMNHF 203
EGL S S GL + D KLY + L+LF GWV S+ + P F
Sbjct: 151 LEGLKSTASENGLSGDLFN----DTKLYGVSLALFSKGWVPRRQRSRPIWQPQARFKKFF 210
Query: 204 GAGKGQLKLKMSPKDRAYYERLVHRNKPPGARRLLQTNYQDDGIVVNGIVGVDQNGMYDF 263
G G+L LKM+ + RA Y + R +LLQ++ D + V+ IV V QNG +F
Sbjct: 211 GFRNGKLPLKMTERARAVYNTVTRR-------KLLQSDA--DAVQVSDIVTVIQNGTGNF 270
Query: 264 TNITAAIAAAPNKTTVAKGYFLIFVAAGIYNETVLVPKEKD------------------- 323
T I AAIAAAPNKT + GYFLI+V AG+Y E V VPK K
Sbjct: 271 TTINAAIAAAPNKTDGSNGYFLIYVTAGLYEEYVEVPKNKRYVMMIGDGINQTVITGNRS 330
Query: 324 -----------TFSVEGTGFLGVNLTITNTAGSAKHQAVALRVSADNVTLYNCIFEGYQD 383
TF + G F+GVN+TI NTAG K QAVALR D Y+C FE YQD
Sbjct: 331 VVDGWTTFNSATFILSGPNFIGVNITIRNTAGPTKGQAVALRSGGDLSVFYSCSFEAYQD 390
Query: 384 TLYTHSLRQFYRECDVYGTVDFIFGNAAVVLQNCNIYARLPMSGQFNALTAQGRTDPNQN 443
TLYTHSLRQFYRECDVYGTVDFIFGNAAVVLQNCN+Y R P GQ N +TAQGRTDPNQN
Sbjct: 391 TLYTHSLRQFYRECDVYGTVDFIFGNAAVVLQNCNLYPRQPRKGQSNEVTAQGRTDPNQN 450
Query: 444 TGTSIHNCTIKATPELAASPAT-KSYLGRPWKQYSRTVYMQSFIDSFIDPVGWKEWDGTL 503
TGT+IH CTI+ +LA S T K+YLGRPWK+YSRTV MQ++ID F++P GW W G
Sbjct: 451 TGTAIHGCTIRPADDLATSNYTVKTYLGRPWKEYSRTVVMQTYIDGFLEPSGWNAWSGDF 510
Query: 504 NLNTSYYAEFNNSGPGCDTSQRASW-AVGVINATVASNFTVSQLLAGDKWLPPTEVPYTG 525
L+T YYAE+NN+GPG DT+ R +W VINAT ASNFTV+ L G+ W+ T VP+ G
Sbjct: 511 ALSTLYYAEYNNTGPGSDTTNRVTWPGYHVINATDASNFTVTNFLVGEGWIGQTGVPFVG 560
BLAST of Cucsa.250450 vs. TAIR10
Match:
AT1G02810.1 (AT1G02810.1 Plant invertase/pectin methylesterase inhibitor superfamily)
HSP 1 Score: 453.4 bits (1165), Expect = 1.9e-127
Identity = 263/581 (45.27%), Postives = 344/581 (59.21%), Query Frame = 1
Query: 5 NPISLLTFLTLLF--LLSPSSSAAPTADICQSTTSPPFCNSFLPKSPDSIHSHCRFTLHQ 64
+PI +L L+ +L+ S + + ++ IC++T P +C S P S ++ + F++ +
Sbjct: 3 SPIFILITLSFFLQSVLASSQTLSNSSTICKTTPDPKYCKSVFPHSQGNVQQYGCFSIRK 62
Query: 65 ALAHARTFLSLV------NAQLNLLPSLSALHDCRCLAEANLDFLFQTFSIVNSTTTT-- 124
+L+ +R F+ V NA L+ + AL DCR LA +D+L +F VN T+
Sbjct: 63 SLSQSRKFIRTVDRYIKRNAHLSQPAVIRALQDCRFLAGLTMDYLLTSFETVNDTSAKTS 122
Query: 125 ---LPYYDAHEMLSLISAIITNVDTCYEGL---ASLNSAVGLVDKVLEAISFDKKLYSLY 184
L + A ++ +L+SA +TN TC EGL AS ++ + V + D KL +
Sbjct: 123 FKPLSFPKADDIQTLLSAALTNEQTCLEGLTTAASYSATWTVRTGVALPLVNDTKLLGVS 182
Query: 185 LSLFKMGWVSKDLKAPTF----PKMNHFGAGK-------GQLKLKMSPKDRAYYERLVHR 244
L+LF GWV K K F P+ K G L LKM+ K +A YE L R
Sbjct: 183 LALFTKGWVPKKKKRAGFAWAQPRSGSSTHTKPFRLFRNGALPLKMTEKTKAVYESLSRR 242
Query: 245 NKPPGARRLLQTNYQDDG--IVVNGIVGVDQNGMYDFTNITAAIAAAPNKTTVAKGYFLI 304
G DDG ++++ IV V Q+G +FTNITAA+AAAPN T + G+FLI
Sbjct: 243 KLADGD----SNGDGDDGSMVLISDIVTVSQDGTGNFTNITAAVAAAPNNTDGSAGFFLI 302
Query: 305 FVAAGIYNETVLVPKEKD------------------------------TFSVEGTGFLGV 364
+V AGIY E + + K K TF+V F+ V
Sbjct: 303 YVTAGIYEEYISIAKNKRYMMMIGDGINQTVVTGNRSVVDGWTTFNSATFAVTAPNFVAV 362
Query: 365 NLTITNTAGSAKHQAVALRVSADNVTLYNCIFEGYQDTLYTHSLRQFYRECDVYGTVDFI 424
N+T NTAG KHQAVALR AD Y+C FE YQDTLYTHSLRQFYRECDVYGTVDFI
Sbjct: 363 NITFRNTAGPEKHQAVALRSGADFSIFYSCSFEAYQDTLYTHSLRQFYRECDVYGTVDFI 422
Query: 425 FGNAAVVLQNCNIYARLPMSGQFNALTAQGRTDPNQNTGTSIHNCTIKATPELAASPAT- 484
FGNAAVV QNCN+Y R PM QFNA+TAQGR+DPNQNTGTSI NCTIK +L +S T
Sbjct: 423 FGNAAVVFQNCNLYPRKPMPNQFNAITAQGRSDPNQNTGTSIQNCTIKPADDLVSSNYTV 482
Query: 485 KSYLGRPWKQYSRTVYMQSFIDSFIDPVGWKEWDGTLNLNTSYYAEFNNSGPGCDTSQRA 525
K+YLGRPWK+YSRTVYMQS+ID F++PVGW+EW+G L+T YYAE+NN+GPG +T+ R
Sbjct: 483 KTYLGRPWKEYSRTVYMQSYIDGFVEPVGWREWNGDFALSTLYYAEYNNTGPGSNTTNRV 542
BLAST of Cucsa.250450 vs. TAIR10
Match:
AT4G02330.1 (AT4G02330.1 Plant invertase/pectin methylesterase inhibitor superfamily)
HSP 1 Score: 448.7 bits (1153), Expect = 4.8e-126
Identity = 263/582 (45.19%), Postives = 342/582 (58.76%), Query Frame = 1
Query: 7 ISLLTFLTLLFL-----------LSPSSSAAPTADICQSTTSPPFCNSFLPK-SPDSIHS 66
+SL FL LFL L PS+S++ IC++T P FC S P+ S +
Sbjct: 2 LSLKLFLVTLFLSLQTLFIASQTLLPSNSSST---ICKTTPDPKFCKSVFPQTSQGDVRE 61
Query: 67 HCRFTLHQALAHARTFLSLVNAQL---NLLPSLSA---LHDCRCLAEANLDFLFQTFSIV 126
+ RF+L ++L +R F ++ L N L S SA L DCR LA D+L +F V
Sbjct: 62 YGRFSLRKSLTQSRKFTRTIDRYLKRNNALLSQSAVGALQDCRYLASLTTDYLITSFETV 121
Query: 127 NSTTT--TLPYYDAHEMLSLISAIITNVDTCYEGL-ASLNSAVGLVDKVLEAISFDKKLY 186
N TT+ TL + A E+ +L+SA +TN TC +G+ + +S+ + + V + D KL+
Sbjct: 122 NITTSSKTLSFSKADEIQTLLSAALTNEQTCLDGINTAASSSWTIRNGVALPLINDTKLF 181
Query: 187 SLYLSLFKMGWVSKDLKAPT-----FPKMNHFGAG------KGQLKLKMSPKDRAYYERL 246
S+ L+LF GWV K K PK H G L LKM+ RA YE L
Sbjct: 182 SVSLALFTKGWVPKKKKQVASYSWAHPKNTHSHTKPFRHFRNGALPLKMTEHTRAVYESL 241
Query: 247 VHRNKPPGARRLLQTNYQDDGIVVNGIVGVDQNGMYDFTNITAAIAAAPNKTTVAKGYFL 306
R+L + + ++V+ IV V+QNG +FT IT A+ +APNKT GYF+
Sbjct: 242 -------SRRKLADDDNDVNTVLVSDIVTVNQNGTGNFTTITEAVNSAPNKTDGTAGYFV 301
Query: 307 IFVAAGIYNETVLVPKEKD------------------------------TFSVEGTGFLG 366
I+V +G+Y E V++ K K TF+V F+
Sbjct: 302 IYVTSGVYEENVVIAKNKRYLMMIGDGINRTVVTGNRNVVDGWTTFNSATFAVTSPNFVA 361
Query: 367 VNLTITNTAGSAKHQAVALRVSADNVTLYNCIFEGYQDTLYTHSLRQFYRECDVYGTVDF 426
VN+T NTAG KHQAVA+R SAD Y+C FE YQDTLYTHSLRQFYRECD+YGTVDF
Sbjct: 362 VNMTFRNTAGPEKHQAVAMRSSADLSIFYSCSFEAYQDTLYTHSLRQFYRECDIYGTVDF 421
Query: 427 IFGNAAVVLQNCNIYARLPMSGQFNALTAQGRTDPNQNTGTSIHNCTIKATPELAASPAT 486
IFGNAAVV Q+CN+Y R PM QFNA+TAQGRTDPNQNTG SIHNCTIK +L +S T
Sbjct: 422 IFGNAAVVFQDCNLYPRQPMQNQFNAITAQGRTDPNQNTGISIHNCTIKPADDLVSSNYT 481
Query: 487 -KSYLGRPWKQYSRTVYMQSFIDSFIDPVGWKEWDGTLNLNTSYYAEFNNSGPGCDTSQR 525
K+YLGRPWK+YSRTV+MQS+ID ++PVGW+EW+G L+T YYAE+NN+G G T+ R
Sbjct: 482 VKTYLGRPWKEYSRTVFMQSYIDEVVEPVGWREWNGDFALSTLYYAEYNNTGSGSSTTDR 541
BLAST of Cucsa.250450 vs. TAIR10
Match:
AT3G10720.2 (AT3G10720.2 Plant invertase/pectin methylesterase inhibitor superfamily)
HSP 1 Score: 373.6 bits (958), Expect = 2.0e-103
Identity = 220/552 (39.86%), Postives = 301/552 (54.53%), Query Frame = 1
Query: 19 LSPSSSAAPTADICQSTTSPPFCNSFL---PKSPDSIHSHCRFTLHQALAHARTFLSLVN 78
L PS S +P+ C+ST P C + L SP + + +FT+ Q L A ++
Sbjct: 71 LPPSQSQSPSL-ACKSTPYPKLCRTILNAVKSSPSDPYRYGKFTIKQCLKQASRLSKVIT 130
Query: 79 AQLNLLPS---------LSALHDCRCLAEANLDFLFQTFSIVNSTTTTLPYYDAHEMLSL 138
+ + S + A+ DC L+E ++++L +T + T + + SL
Sbjct: 131 SYARRVESKPGSATAEEIGAVADCGELSELSVNYL-ETVTTELKTAQVMTAALVEHVNSL 190
Query: 139 ISAIITNVDTCYEGLASLNSAVGLVDKVLEAISFDKKLYSLYLSLFKMGWVSKDLKAPTF 198
+S ++TN TC +GL S G + + +LYS+ L L ++++LK
Sbjct: 191 LSGVVTNQQTCLDGLVEAKS--GFAAAIGSPMGNLTRLYSISLGLVSHA-LNRNLKRFKA 250
Query: 199 PKMNHFGAGKGQLKLKMSPKDRAYYERLVHRNKPPGARRLLQTNYQDDG--IVVNGIVGV 258
K G G + + + + + R L + G I+V+ V V
Sbjct: 251 SKGKILGGGNSTYREPLETLIKGLRKTCDNDKDCRKTSRNLGELGETSGGSILVSKAVIV 310
Query: 259 DQNGMYDFTNITAAIAAAPNKTTVAKGYFLIFVAAGIYNETVLVPKEK------------ 318
+FT IT AIAAAPN T GYF+I+ G+Y E ++VP K
Sbjct: 311 GPFKSDNFTTITDAIAAAPNNTRPEDGYFVIYAREGVYEEYIVVPINKKNLMLMGDGINK 370
Query: 319 ------------------DTFSVEGTGFLGVNLTITNTAGSAKHQAVALRVSADNVTLYN 378
+F+V G F+ V++T NTAG KHQAVALR +A+ + Y
Sbjct: 371 TIITGNHNVVDGWTTYNCSSFAVVGERFMAVDVTFRNTAGPEKHQAVALRNNAEGSSFYR 430
Query: 379 CIFEGYQDTLYTHSLRQFYRECDVYGTVDFIFGNAAVVLQNCNIYARLPMSGQFNALTAQ 438
C FEGYQDTLY HSLRQFYRECD+YGTVDFIFGNAA + QNCNIYAR PM+ Q NA+TA
Sbjct: 431 CSFEGYQDTLYVHSLRQFYRECDIYGTVDFIFGNAAAIFQNCNIYARKPMAKQKNAITAH 490
Query: 439 GRTDPNQNTGTSIHNCTIKATPELAASP-ATKSYLGRPWKQYSRTVYMQSFIDSFIDPVG 498
GR DPNQNTG SI NCTIKA P+LAA P + ++LGRPWK YSRTV+MQS+I + PVG
Sbjct: 491 GRLDPNQNTGISIINCTIKAAPDLAAEPKSAMTFLGRPWKPYSRTVFMQSYISDIVQPVG 550
Query: 499 WKEWDGTLNLNTSYYAEFNNSGPGCDTSQRASW-AVGVINATVASNFTVSQLLAGDKWLP 525
W EW+GT+ L+T YY E++N GPG +T+QR W ++N A NFTV GD WLP
Sbjct: 551 WLEWNGTIGLDTIYYGEYSNFGPGANTNQRVQWLGYNLLNLAEAMNFTVYNFTMGDTWLP 610
BLAST of Cucsa.250450 vs. TAIR10
Match:
AT5G04970.1 (AT5G04970.1 Plant invertase/pectin methylesterase inhibitor superfamily)
HSP 1 Score: 369.0 bits (946), Expect = 4.8e-102
Identity = 222/551 (40.29%), Postives = 299/551 (54.26%), Query Frame = 1
Query: 20 SPSS-SAAPTADICQSTTSPPFCNSFLP---KSPDSIHSHCRFTLHQALAHARTFLSLVN 79
SPS S P C+ST P C + L SP + + +FT+ Q L AR ++N
Sbjct: 75 SPSQPSPLPPNIACKSTPYPKLCRTILSAVKSSPSDPYHYGKFTMKQCLKQARRLSKVIN 134
Query: 80 --AQ-------LNLLPSLSALHDCRCLAEANLDFLFQTFSIVNSTTTTLPYYDAHEMLSL 139
AQ + + +SA+ DC LAE ++++L +T + + + SL
Sbjct: 135 RFAQRVEADPGTSTVEEVSAVADCGELAELSVEYL-ETVTEELKAAELMTAALVDRVTSL 194
Query: 140 ISAIITNVDTCYEGLASLNSAVGLVDKVLEAISFDKKLYSLYLSLFKMGWVSKDLKAPTF 199
+ ++TN TC +GL S G + + +LYS+ L L ++++LK
Sbjct: 195 LGGVVTNQQTCLDGLVDAKS--GFATAIGTPLGNLTRLYSVSLGLVSHA-LNRNLKRYKG 254
Query: 200 PKMNHFGAGKGQLKLKMSPKDRAYYERLVHRNKPPGARRLLQTNYQDDG--IVVNGIVGV 259
K FG G ++ + + + A R L + G I+V V V
Sbjct: 255 SKGKIFGGGNKPVREPLETLIKVLRKTCDKGKDCRKANRNLGELGETSGGSILVREAVTV 314
Query: 260 DQNGMYDFTNITAAIAAAPNKTTVAKGYFLIFVAAGIYNETVLV---------------- 319
+F IT A+AAAPN T +GYF+I+ AG+Y E V++
Sbjct: 315 GPYETDNFPTITEAVAAAPNHTFPEQGYFVIYARAGLYEEYVVISNKKRNIMLIGDGINK 374
Query: 320 --------------PKEKDTFSVEGTGFLGVNLTITNTAGSAKHQAVALRVSADNVTLYN 379
TF+V G F+ V++T NTAG KHQAVA+R +AD T Y
Sbjct: 375 TIISGNHSFIDGWTTYNSSTFAVVGDRFVAVDVTFRNTAGPEKHQAVAVRNNADGSTFYR 434
Query: 380 CIFEGYQDTLYTHSLRQFYRECDVYGTVDFIFGNAAVVLQNCNIYARLPMSGQFNALTAQ 439
C FEGYQDTLY HSLRQFYRECD+YGT+DFIFGNAA + QNCNIYAR PM+ Q NA+TA
Sbjct: 435 CSFEGYQDTLYVHSLRQFYRECDIYGTIDFIFGNAAAIFQNCNIYARKPMANQKNAVTAH 494
Query: 440 GRTDPNQNTGTSIHNCTIKATPELAASP-ATKSYLGRPWKQYSRTVYMQSFIDSFIDPVG 499
GRTDPNQ TG SI NCTI A P+LAA P +T ++LGRPWK YSRTVY+QS+I + PVG
Sbjct: 495 GRTDPNQKTGISIINCTIGAAPDLAADPKSTMTFLGRPWKPYSRTVYIQSYISDVVQPVG 554
Query: 500 WKEWDGTLNLNTSYYAEFNNSGPGCDTSQRASWA-VGVINATVASNFTVSQLLAGDKWLP 524
W EW+GT L+T Y E++N GPG DTS+R W+ ++N A NFTV GD WLP
Sbjct: 555 WLEWNGTTGLDTISYGEYDNFGPGADTSKRVQWSGYSLLNLVQAMNFTVYNFTLGDTWLP 614
BLAST of Cucsa.250450 vs. NCBI nr
Match:
gi|449461481|ref|XP_004148470.1| (PREDICTED: pectinesterase-like [Cucumis sativus])
HSP 1 Score: 1038.5 bits (2684), Expect = 4.0e-300
Identity = 521/554 (94.04%), Postives = 522/554 (94.22%), Query Frame = 1
Query: 1 MVSSNPISLLTFLTLLFLLSPSSSAAPTADICQSTTSPPFCNSFLPKSPDSIHSHCRFTL 60
MVSSNPISLLTFLTLLFLLSPSSSAAPTADICQSTTSPPFCNSFLPKSPDSIHSHCRFTL
Sbjct: 1 MVSSNPISLLTFLTLLFLLSPSSSAAPTADICQSTTSPPFCNSFLPKSPDSIHSHCRFTL 60
Query: 61 HQALAHARTFLSLVNAQLNLLPSLSALHDCRCLAEANLDFLFQTFSIVNSTTTTLPYYDA 120
HQALAHARTFLSLVNAQLNLLPSLSALHDCRCLAEANLDFLFQTFSIVNSTTTTLPYYDA
Sbjct: 61 HQALAHARTFLSLVNAQLNLLPSLSALHDCRCLAEANLDFLFQTFSIVNSTTTTLPYYDA 120
Query: 121 HEMLSLISAIITNVDTCYEGLASLNSAVGLVDKVLEAISFDKKLYSLYLSLFKMGWVSKD 180
HEMLSLISAIITNVDTCYEGLASLNSAVGLVDKVLEAISFDKKLYSLYLSLFKMGWVSKD
Sbjct: 121 HEMLSLISAIITNVDTCYEGLASLNSAVGLVDKVLEAISFDKKLYSLYLSLFKMGWVSKD 180
Query: 181 LKAPTFPKMNHFGAGKGQLKLKMSPKDRAYYERLVHRNKPPGARRLLQTNYQDDGIVVNG 240
LKAPTFPKMNHFGAGKGQLKLKMSPKDRAYYERLVHRNKPPGARRLLQTNYQDDGIVVNG
Sbjct: 181 LKAPTFPKMNHFGAGKGQLKLKMSPKDRAYYERLVHRNKPPGARRLLQTNYQDDGIVVNG 240
Query: 241 IVGVDQNGMYDFTNITAAIAAAPNKTTVAKGYFLIFVAAGIYNETVLVPKEK-------- 300
IVGVDQNGMYDFTNITAAIAAAPNKTTVAKGYFLIFVAAGIYNETVLVPKEK
Sbjct: 241 IVGVDQNGMYDFTNITAAIAAAPNKTTVAKGYFLIFVAAGIYNETVLVPKEKRYVLLIGE 300
Query: 301 ----------------------DTFSVEGTGFLGVNLTITNTAGSAKHQAVALRVSADNV 360
T +VEGTGFLGVNLTITNTAGSAKHQAVALRVSADNV
Sbjct: 301 GNNQTIITGNKNVVDGSTTFNSATVAVEGTGFLGVNLTITNTAGSAKHQAVALRVSADNV 360
Query: 361 TLYNCIFEGYQDTLYTHSLRQFYRECDVYGTVDFIFGNAAVVLQNCNIYARLPMSGQFNA 420
TLYNCIFEGYQDTLYTHSLRQFYRECDVYGTVDFIFGNAAVVLQNCNIYARLPMSGQFNA
Sbjct: 361 TLYNCIFEGYQDTLYTHSLRQFYRECDVYGTVDFIFGNAAVVLQNCNIYARLPMSGQFNA 420
Query: 421 LTAQGRTDPNQNTGTSIHNCTIKATPELAASPATKSYLGRPWKQYSRTVYMQSFIDSFID 480
LTAQGRTDPNQNTGTSIHNCTIKATPELAASPATKSYLGRPWKQYSRTVYMQSFIDSFID
Sbjct: 421 LTAQGRTDPNQNTGTSIHNCTIKATPELAASPATKSYLGRPWKQYSRTVYMQSFIDSFID 480
Query: 481 PVGWKEWDGTLNLNTSYYAEFNNSGPGCDTSQRASWAVGVINATVASNFTVSQLLAGDKW 525
PVGWKEWDGTLNLNTSYYAEFNNSGPGCDTSQRASWAVGVINATVASNFTVSQLLAGDKW
Sbjct: 481 PVGWKEWDGTLNLNTSYYAEFNNSGPGCDTSQRASWAVGVINATVASNFTVSQLLAGDKW 540
BLAST of Cucsa.250450 vs. NCBI nr
Match:
gi|659116086|ref|XP_008457895.1| (PREDICTED: pectinesterase-like [Cucumis melo])
HSP 1 Score: 963.0 bits (2488), Expect = 2.1e-277
Identity = 478/555 (86.13%), Postives = 498/555 (89.73%), Query Frame = 1
Query: 1 MVSSNPISLLTFLTLLFLLSPSSSAAPTADICQSTTSPPFCNSFLPKSPDSIHSHCRFTL 60
MVSSNPIS+LTFLTLLF LSPSSSA PTA+ICQSTTSPPFCN FLP SP S+HSHCRFTL
Sbjct: 1 MVSSNPISVLTFLTLLFFLSPSSSAVPTAEICQSTTSPPFCNFFLPNSPASVHSHCRFTL 60
Query: 61 HQALAHARTFLSLVNAQLNLLPSLSALHDCRCLAEANLDFLFQTFSIVNSTTTTL-PYYD 120
H AL HARTFLSLVNA LNL PSLS LHDCR LAEANLDFL QTFSIVNSTTTTL PYYD
Sbjct: 61 HHALVHARTFLSLVNAHLNLFPSLSPLHDCRSLAEANLDFLSQTFSIVNSTTTTLLPYYD 120
Query: 121 AHEMLSLISAIITNVDTCYEGLASLNSAVGLVDKVLEAISFDKKLYSLYLSLFKMGWVSK 180
AHE L+L+SA+ITN DTCYEGLA L+SAVGLVDKVLEAISFDKKLYSLYLSLFKMGWVSK
Sbjct: 121 AHEKLTLMSAVITNEDTCYEGLAGLDSAVGLVDKVLEAISFDKKLYSLYLSLFKMGWVSK 180
Query: 181 DLKAPTFPKMNHFGAGKGQLKLKMSPKDRAYYERLVHRNKPPGARRLLQTNYQDDGIVVN 240
DLKAPT PKM HFG GKGQLKLKMSPKDRAYYERLVHRNKPPGARRLLQTNYQDDGIV+N
Sbjct: 181 DLKAPTLPKMKHFGVGKGQLKLKMSPKDRAYYERLVHRNKPPGARRLLQTNYQDDGIVIN 240
Query: 241 GIVGVDQNGMYDFTNITAAIAAAPNKTTVAKGYFLIFVAAGIYNETVLVPKEK------- 300
GIVGVDQNG YDFTNITAAIAAAPNKTTVAKGYFLIFVAAGIYNETVL+PKEK
Sbjct: 241 GIVGVDQNGTYDFTNITAAIAAAPNKTTVAKGYFLIFVAAGIYNETVLIPKEKRYVLLIG 300
Query: 301 -----------------------DTFSVEGTGFLGVNLTITNTAGSAKHQAVALRVSADN 360
T +VEGTGFLGVNLTI+NTAG+AKHQAVALRVSADN
Sbjct: 301 EGNNQTIITGNKNVVDGSTTFNSATVAVEGTGFLGVNLTISNTAGAAKHQAVALRVSADN 360
Query: 361 VTLYNCIFEGYQDTLYTHSLRQFYRECDVYGTVDFIFGNAAVVLQNCNIYARLPMSGQFN 420
TLYNCIFEGYQDTLYTHSLRQFYRECD+YGTVDFIFGNAAVVLQNCNIYARLP+SGQFN
Sbjct: 361 ATLYNCIFEGYQDTLYTHSLRQFYRECDIYGTVDFIFGNAAVVLQNCNIYARLPLSGQFN 420
Query: 421 ALTAQGRTDPNQNTGTSIHNCTIKATPELAASPATKSYLGRPWKQYSRTVYMQSFIDSFI 480
A+TAQGRTDPNQNTGTSIHNCTIKATPELAASPATKSYLGRPWKQYSRTVYMQSFIDSFI
Sbjct: 421 AVTAQGRTDPNQNTGTSIHNCTIKATPELAASPATKSYLGRPWKQYSRTVYMQSFIDSFI 480
Query: 481 DPVGWKEWDGTLNLNTSYYAEFNNSGPGCDTSQRASWAVGVINATVASNFTVSQLLAGDK 525
DP GWKEWDGT+NLNTSYYAEFNNSGPG DTSQR SWAVGVINATVA++FTVSQ LAGD+
Sbjct: 481 DPAGWKEWDGTMNLNTSYYAEFNNSGPGSDTSQRVSWAVGVINATVANSFTVSQFLAGDR 540
BLAST of Cucsa.250450 vs. NCBI nr
Match:
gi|700206964|gb|KGN62083.1| (hypothetical protein Csa_2G296080 [Cucumis sativus])
HSP 1 Score: 585.5 bits (1508), Expect = 9.3e-164
Identity = 302/352 (85.80%), Postives = 312/352 (88.64%), Query Frame = 1
Query: 1 MVSSNPISLLTFLTLLFLLSPSSSAAPTADICQSTTSPPFCNSFLPKSPDSIHSHCRFTL 60
MVSSNPISLLTFLTLLFLLSPSSSAAPTADICQSTTSPPFCNSFLPKSPDSIHSHCRFTL
Sbjct: 1 MVSSNPISLLTFLTLLFLLSPSSSAAPTADICQSTTSPPFCNSFLPKSPDSIHSHCRFTL 60
Query: 61 HQALAHARTFLSLVNAQLNLLPSLSALHDCRCLAEANLDFLFQTFSIVNSTTTTLPYYDA 120
HQALAHARTFLSLVNAQLNLLPSLSALHDCRCLAEANLDFLFQTFSIVNSTTTTLPYYDA
Sbjct: 61 HQALAHARTFLSLVNAQLNLLPSLSALHDCRCLAEANLDFLFQTFSIVNSTTTTLPYYDA 120
Query: 121 HEMLSLISAIITNVDTCYEGLASLNSAVGLVDKVLEAISFDKKLYSLYLSLFKMGWVSKD 180
HEMLSLISAIITNVDTCYEGLASLNSAVGLVDKVLEAISFDKKLYSLYLSLFKMGWVSKD
Sbjct: 121 HEMLSLISAIITNVDTCYEGLASLNSAVGLVDKVLEAISFDKKLYSLYLSLFKMGWVSKD 180
Query: 181 LKAPTFPKMNHFGAGKGQLKLKMSPKDRAYYERLVHRNKPPGARRLLQTNYQDDGIVVNG 240
LKAPTFPKMNHFGAGKGQLKLKMSPKDRAYYERLVHRNKPPGARRLLQTNYQDDGIVVNG
Sbjct: 181 LKAPTFPKMNHFGAGKGQLKLKMSPKDRAYYERLVHRNKPPGARRLLQTNYQDDGIVVNG 240
Query: 241 IVGVDQNGMYDFTNITAAIAAAPNKTTVAKGYFLIFVAAGIYNETVLVPKEKDTFSVEGT 300
IVGVDQNGMYDFTNITAAIAAAPNKTTVAKGYFLIFVAAGIYNETVLVPKEK + G
Sbjct: 241 IVGVDQNGMYDFTNITAAIAAAPNKTTVAKGYFLIFVAAGIYNETVLVPKEKRYVLLIGE 300
Query: 301 G----FLGVNLTITNTAGSAKHQAVALRVSADNVTLYNCIFEGYQDTLYTHS 349
G + N + + + + V L S + ++ Y T+Y S
Sbjct: 301 GNNQTIITGNKNVVDGSTTFNSATVELAASPATKSYLGRPWKQYSRTVYMQS 352
BLAST of Cucsa.250450 vs. NCBI nr
Match:
gi|700206964|gb|KGN62083.1| (hypothetical protein Csa_2G296080 [Cucumis sativus])
HSP 1 Score: 232.3 bits (591), Expect = 2.0e-57
Identity = 110/111 (99.10%), Postives = 110/111 (99.10%), Query Frame = 1
Query: 414 ATPELAASPATKSYLGRPWKQYSRTVYMQSFIDSFIDPVGWKEWDGTLNLNTSYYAEFNN 473
AT ELAASPATKSYLGRPWKQYSRTVYMQSFIDSFIDPVGWKEWDGTLNLNTSYYAEFNN
Sbjct: 323 ATVELAASPATKSYLGRPWKQYSRTVYMQSFIDSFIDPVGWKEWDGTLNLNTSYYAEFNN 382
Query: 474 SGPGCDTSQRASWAVGVINATVASNFTVSQLLAGDKWLPPTEVPYTGGLIS 525
SGPGCDTSQRASWAVGVINATVASNFTVSQLLAGDKWLPPTEVPYTGGLIS
Sbjct: 383 SGPGCDTSQRASWAVGVINATVASNFTVSQLLAGDKWLPPTEVPYTGGLIS 433
HSP 2 Score: 526.6 bits (1355), Expect = 5.1e-146
Identity = 286/560 (51.07%), Postives = 362/560 (64.64%), Query Frame = 1
Query: 7 ISLLTFLTLLFLLS---PSSSAAPTADICQSTTSPPFCNSFLPKSPDSIHSHCRFTLHQA 66
+ +L FL L L+ P++S +P IC+ST P +CNS LP +++ + RF++ ++
Sbjct: 9 VIILIFLPSLLALADITPNTSVSP-GTICKSTPDPSYCNSVLPPQNGNVYEYGRFSVRKS 68
Query: 67 LAHARTFLSLVNAQLNL-------LPSLSALHDCRCLAEANLDFLFQTFSIVNSTTTTLP 126
L+ A FL+LVN L L P++ AL DC+ LAE N+DFL + VN TT LP
Sbjct: 69 LSQATNFLNLVNRYLQLQRRSSLSTPAIHALEDCQSLAELNIDFLSSSLETVNRTTKFLP 128
Query: 127 YYDAHEMLSLISAIITNVDTCYEGLASLNSAVGLVDKVLEAISFDKKLYSLYLSLFKMGW 186
A ++ +L+SAI+TN TC EGL + SA L + + +S D KLYS+ L+LF GW
Sbjct: 129 TSQADDIQTLLSAILTNQQTCLEGLQATASAWRLKNGLSVPLSNDTKLYSVSLALFTKGW 188
Query: 187 VSKDLKAPTF-PKMNHFGAGKGQLKLKMSPKDRAYYERLVHRNKPPGARRLLQTNYQDDG 246
V ++ F P H G G+L LKMS + RA YE + R+LLQ D+
Sbjct: 189 VPENANVTAFQPSAKHRGFRNGRLPLKMSSRTRAIYESV-------SRRKLLQATVGDE- 248
Query: 247 IVVNGIVGVDQNGMYDFTNITAAIAAAPNKTTVAKGYFLIFVAAGIYNETVLVPKEKD-- 306
+ V IV V ++G +FT I+ A+AAAPNKT+ GYFLI+V AG+Y E V + K+K
Sbjct: 249 VKVKDIVTVSKDGNGNFTTISDAVAAAPNKTSSTAGYFLIYVTAGVYEENVSIDKKKTYL 308
Query: 307 ----------------------------TFSVEGTGFLGVNLTITNTAGSAKHQAVALRV 366
TF+V G F+GVN+TI NTAG+ KHQAVALR
Sbjct: 309 MMVGDGINKTIITGNRSVVDGWTTFKSATFAVVGARFVGVNMTIRNTAGAEKHQAVALRN 368
Query: 367 SADNVTLYNCIFEGYQDTLYTHSLRQFYRECDVYGTVDFIFGNAAVVLQNCNIYARLPMS 426
AD T Y+C FEGYQDTLYTHSLRQFYRECD+YGTVDFIFGNAAVV QNCN+Y RLPMS
Sbjct: 369 GADLSTFYSCSFEGYQDTLYTHSLRQFYRECDIYGTVDFIFGNAAVVFQNCNLYPRLPMS 428
Query: 427 GQFNALTAQGRTDPNQNTGTSIHNCTIKATPELAAS-PATKSYLGRPWKQYSRTVYMQSF 486
GQFN++TAQGRTDPNQNTGTSIHNCTI+ +LAA+ A ++YLGRPWK YSRTVYMQSF
Sbjct: 429 GQFNSITAQGRTDPNQNTGTSIHNCTIRPADDLAANIDAAETYLGRPWKNYSRTVYMQSF 488
Query: 487 IDSFIDPVGWKEWDGTLNLNTSYYAEFNNSGPGCDTSQRASW-AVGVINATVASNFTVSQ 524
+D+ I+ GW+EWDG L+T YYAEFNN+GPG T+ R +W VINATVA+NFTV+
Sbjct: 489 MDTVINSAGWREWDGDFALSTLYYAEFNNTGPGSTTANRVTWPGYHVINATVAANFTVAN 548
BLAST of Cucsa.250450 vs. NCBI nr
Match:
gi|645258934|ref|XP_008235120.1| (PREDICTED: probable pectinesterase/pectinesterase inhibitor 7 [Prunus mume])
HSP 1 Score: 525.8 bits (1353), Expect = 8.7e-146
Identity = 287/563 (50.98%), Postives = 364/563 (64.65%), Query Frame = 1
Query: 1 MVSSNPISLLTFLTLLFLLSPSSSAAPTADICQSTTSPPFCNSFLP--KSPDSIHSHCRF 60
+V+S I L F +L + SSS IC+ST P FC S LP ++ +++ + RF
Sbjct: 7 IVASFFILLPFFASLSLAANDSSSPVSAGTICKSTPDPSFCKSVLPHNQTAQNVYYYGRF 66
Query: 61 TLHQALAHARTFLSLVNAQLNLLPSLS-----ALHDCRCLAEANLDFLFQTFSIVNSTTT 120
++ ++L+ +R FL+LV+ L SLS AL DCR LA N+DFL +F VN T+
Sbjct: 67 SVRKSLSQSRKFLNLVDKYLKRRSSLSLTAVRALEDCRLLAGLNMDFLLNSFETVNKTSK 126
Query: 121 TLPYYDAHEMLSLISAIITNVDTCYEGLASLNSAVGLVDKVLEAISFDKKLYSLYLSLFK 180
TL A ++ + +SAI+TN TC +G+ S SA L K + D K YS+ L+LF
Sbjct: 127 TLSNLKADDVQTSLSAILTNQQTCLDGIQSTASAWSL--KTGLSAPNDTKFYSVSLALFT 186
Query: 181 MGWVSKDLKAPTF-PKMNHFGAGKGQLKLKMSPKDRAYYERLVHRNKPPGARRLLQTNYQ 240
GWV K K+ T+ P N G G+L LKMS + RA YE + R ++LQT +
Sbjct: 187 KGWVPKKKKSATWKPTRNQRGFKNGRLPLKMSSRTRAVYEGVTKR-------KMLQTETE 246
Query: 241 DDGIVVNGIVGVDQNGMYDFTNITAAIAAAPNKTTVAKGYFLIFVAAGIYNETVLVPKEK 300
DDG++V+ +V V QNG +F+ I AIAAAPN + GYFLI+V AG+Y E V + K K
Sbjct: 247 DDGVLVSEVVTVSQNGTGNFSTINDAIAAAPNNSASTDGYFLIYVTAGVYEEYVSIAKNK 306
Query: 301 D------------------------------TFSVEGTGFLGVNLTITNTAGSAKHQAVA 360
TF+V GF+ VN+T NTAG+ KHQAVA
Sbjct: 307 RYLMMVGDGINQTILTGNRSVVDGWTTFNSATFAVTAPGFVAVNMTFRNTAGAIKHQAVA 366
Query: 361 LRVSADNVTLYNCIFEGYQDTLYTHSLRQFYRECDVYGTVDFIFGNAAVVLQNCNIYARL 420
+R AD T Y+C FE YQDTLYTHSLRQFYRECD+YGTVDFIFGNAAVV QNCN+Y RL
Sbjct: 367 VRNGADLSTFYSCSFEAYQDTLYTHSLRQFYRECDIYGTVDFIFGNAAVVFQNCNLYPRL 426
Query: 421 PMSGQFNALTAQGRTDPNQNTGTSIHNCTIKATPELAAS-PATKSYLGRPWKQYSRTVYM 480
PMSGQFNA+TAQGRTDPNQNTGTSIHNCTI+A +LA+S ++K+YLGRPWK+YSRTVYM
Sbjct: 427 PMSGQFNAITAQGRTDPNQNTGTSIHNCTIRAADDLASSNSSSKTYLGRPWKEYSRTVYM 486
Query: 481 QSFIDSFIDPVGWKEWDGTLNLNTSYYAEFNNSGPGCDTSQRASW-AVGVINATVASNFT 524
QS+IDS +DP GW+ WDG L+T YYAE+NN+GPG +T+ R +W VINAT A+NFT
Sbjct: 487 QSYIDSVVDPAGWRAWDGEFALSTLYYAEYNNTGPGSNTTSRVTWTGYHVINATDAANFT 546
The following BLAST results are available for this feature:
| Match Name | E-value | Identity | Description | |
|---|
| PME20_ARATH | 8.8e-130 | 47.79 | Probable pectinesterase/pectinesterase inhibitor 20 OS=Arabidopsis thaliana GN=P... | [more] |
| PME7_ARATH | 3.5e-126 | 45.27 | Probable pectinesterase/pectinesterase inhibitor 7 OS=Arabidopsis thaliana GN=PM... | [more] |
| PME41_ARATH | 8.5e-125 | 45.19 | Probable pectinesterase/pectinesterase inhibitor 41 OS=Arabidopsis thaliana GN=P... | [more] |
| PME25_ARATH | 3.5e-102 | 39.86 | Probable pectinesterase/pectinesterase inhibitor 25 OS=Arabidopsis thaliana GN=P... | [more] |
| PME47_ARATH | 8.6e-101 | 40.29 | Probable pectinesterase/pectinesterase inhibitor 47 OS=Arabidopsis thaliana GN=P... | [more] |
| Match Name | E-value | Identity | Description | |
|---|
| A0A0A0LJX5_CUCSA | 6.5e-164 | 85.80 | Pectinesterase OS=Cucumis sativus GN=Csa_2G296080 PE=4 SV=1 | [more] |
| A0A0A0LJX5_CUCSA | 1.4e-57 | 99.10 | Pectinesterase OS=Cucumis sativus GN=Csa_2G296080 PE=4 SV=1 | [more] |
| B9SAU3_RICCO | 1.4e-145 | 50.27 | Pectinesterase OS=Ricinus communis GN=RCOM_1179060 PE=4 SV=1 | [more] |
| M5VIT6_PRUPE | 1.8e-145 | 51.45 | Pectinesterase OS=Prunus persica GN=PRUPE_ppa003639mg PE=4 SV=1 | [more] |
| A0A0L9VHI5_PHAAN | 2.6e-144 | 50.81 | Pectinesterase OS=Phaseolus angularis GN=LR48_Vigan10g028400 PE=4 SV=1 | [more] |
| Match Name | E-value | Identity | Description | |
|---|
| AT2G47550.1 | 4.9e-131 | 47.79 | Plant invertase/pectin methylesterase inhibitor superfamily | [more] |
| AT1G02810.1 | 1.9e-127 | 45.27 | Plant invertase/pectin methylesterase inhibitor superfamily | [more] |
| AT4G02330.1 | 4.8e-126 | 45.19 | Plant invertase/pectin methylesterase inhibitor superfamily | [more] |
| AT3G10720.2 | 2.0e-103 | 39.86 | Plant invertase/pectin methylesterase inhibitor superfamily | [more] |
| AT5G04970.1 | 4.8e-102 | 40.29 | Plant invertase/pectin methylesterase inhibitor superfamily | [more] |
