CmaCh20G011150 (gene) Cucurbita maxima (Rimu)

BLAST of CmaCh20G011150 vs. Swiss-Prot
Match: PME20_ARATH (Probable pectinesterase/pectinesterase inhibitor 20 OS=Arabidopsis thaliana GN=PME20 PE=2 SV=2)

HSP 1 Score: 518.5 bits (1334), Expect = 9.2e-146
Identity = 283/569 (49.74%), Postives = 372/569 (65.38%), Query Frame = 1

Query: 16  LLFLFLFTSSSAVPTV----------------EVCQSATSPSFCKSFLPSSPASVHSHCR 75
           L+FLF     S++P+                  +C+ A  PS+C+S LP+ P  ++S+ R
Sbjct: 5   LMFLFTLACLSSLPSPFISAQIPAIGNATSPSNICRFAPDPSYCRSVLPNQPGDIYSYGR 64

Query: 76  YTLHHALVQARTFITLVDLQL------TLLPSFSALHDCRSLAMANLDFISSTVNTVNTT 135
            +L  +L +AR FI+++D +L          +  AL DC+ LA   +D++ S+  T ++T
Sbjct: 65  LSLRRSLSRARRFISMIDAELDRKGKVAAKSTVGALEDCKFLASLTMDYLLSSSQTADST 124

Query: 136 TNALPFYQADEMISLLSAVITNVDTCHDGL--AGSGLGLVDKVVKAISFDRQLYSLYLSL 195
              L   +A+++ + LSA ITN  TC +GL    S  GL   +      D +LY + L+L
Sbjct: 125 -KTLSLSRAEDVHTFLSAAITNEQTCLEGLKSTASENGLSGDLFN----DTKLYGVSLAL 184

Query: 196 FKMGWVSKTMKA-----PKLPKTNHFGFGNGPLKLKMSPKDRAYYERLMHGKKPAARRLL 255
           F  GWV +  ++     P+      FGF NG L LKM+ + RA Y  +        R+LL
Sbjct: 185 FSKGWVPRRQRSRPIWQPQARFKKFFGFRNGKLPLKMTERARAVYNTVTR------RKLL 244

Query: 256 QTNNADDGILVNGVVAVDQNGAYDFTTINDAIAAAPNKSTAANGYFLIFVTAGIYMESVV 315
           Q++   D + V+ +V V QNG  +FTTIN AIAAAPNK+  +NGYFLI+VTAG+Y E V 
Sbjct: 245 QSDA--DAVQVSDIVTVIQNGTGNFTTINAAIAAAPNKTDGSNGYFLIYVTAGLYEEYVE 304

Query: 316 VPKSKKYVLMIGEGNNRTIITGNKNVVDGSTTFNSATVIVEGTGFFGANLTITNTAGAIK 375
           VPK+K+YV+MIG+G N+T+ITGN++VVDG TTFNSAT I+ G  F G N+TI NTAG  K
Sbjct: 305 VPKNKRYVMMIGDGINQTVITGNRSVVDGWTTFNSATFILSGPNFIGVNITIRNTAGPTK 364

Query: 376 HQAVALRVSADMTTFYSCIFEGYQDTLYAHSLRQFYRECDIYGTVDFIFGNAAVVLQNCN 435
            QAVALR   D++ FYSC FE YQDTLY HSLRQFYRECD+YGTVDFIFGNAAVVLQNCN
Sbjct: 365 GQAVALRSGGDLSVFYSCSFEAYQDTLYTHSLRQFYRECDVYGTVDFIFGNAAVVLQNCN 424

Query: 436 IYARLPLSGQFNALTAQGRTDQNQNTGIFIHKCTIKPTPDLAASP-TTKSYLGRPWKEFS 495
           +Y R P  GQ N +TAQGRTD NQNTG  IH CTI+P  DLA S  T K+YLGRPWKE+S
Sbjct: 425 LYPRQPRKGQSNEVTAQGRTDPNQNTGTAIHGCTIRPADDLATSNYTVKTYLGRPWKEYS 484

Query: 496 RTVYMQSFIDAFIDPAGWKEWDGTLNLNTSYYAEFDNSGPGSDTSRRAQW-AVGVINATV 554
           RTV MQ++ID F++P+GW  W G   L+T YYAE++N+GPGSDT+ R  W    VINAT 
Sbjct: 485 RTVVMQTYIDGFLEPSGWNAWSGDFALSTLYYAEYNNTGPGSDTTNRVTWPGYHVINATD 544

BLAST of CmaCh20G011150 vs. Swiss-Prot
Match: PME41_ARATH (Probable pectinesterase/pectinesterase inhibitor 41 OS=Arabidopsis thaliana GN=PME41 PE=2 SV=2)

HSP 1 Score: 509.2 bits (1310), Expect = 5.6e-143
Identity = 287/578 (49.65%), Postives = 372/578 (64.36%), Query Frame = 1

Query: 8   ISLLTFFSLLFLFLFTSSSAVPTV-------EVCQSATSPSFCKSFLP-SSPASVHSHCR 67
           +SL  F   LFL L T   A  T+        +C++   P FCKS  P +S   V  + R
Sbjct: 2   LSLKLFLVTLFLSLQTLFIASQTLLPSNSSSTICKTTPDPKFCKSVFPQTSQGDVREYGR 61

Query: 68  YTLHHALVQARTFITLVDLQL----TLLP--SFSALHDCRSLAMANLDFISSTVNTVNTT 127
           ++L  +L Q+R F   +D  L     LL   +  AL DCR LA    D++ ++  TVN T
Sbjct: 62  FSLRKSLTQSRKFTRTIDRYLKRNNALLSQSAVGALQDCRYLASLTTDYLITSFETVNIT 121

Query: 128 TNA--LPFYQADEMISLLSAVITNVDTCHDGL---AGSGLGLVDKVVKAISFDRQLYSLY 187
           T++  L F +ADE+ +LLSA +TN  TC DG+   A S   + + V   +  D +L+S+ 
Sbjct: 122 TSSKTLSFSKADEIQTLLSAALTNEQTCLDGINTAASSSWTIRNGVALPLINDTKLFSVS 181

Query: 188 LSLFKMGWVSKTMKAPKL-----PKTNHFG------FGNGPLKLKMSPKDRAYYERLMHG 247
           L+LF  GWV K  K         PK  H        F NG L LKM+   RA YE L   
Sbjct: 182 LALFTKGWVPKKKKQVASYSWAHPKNTHSHTKPFRHFRNGALPLKMTEHTRAVYESL--- 241

Query: 248 KKPAARRLLQTNNADDGILVNGVVAVDQNGAYDFTTINDAIAAAPNKSTAANGYFLIFVT 307
              + R+L   +N  + +LV+ +V V+QNG  +FTTI +A+ +APNK+    GYF+I+VT
Sbjct: 242 ---SRRKLADDDNDVNTVLVSDIVTVNQNGTGNFTTITEAVNSAPNKTDGTAGYFVIYVT 301

Query: 308 AGIYMESVVVPKSKKYVLMIGEGNNRTIITGNKNVVDGSTTFNSATVIVEGTGFFGANLT 367
           +G+Y E+VV+ K+K+Y++MIG+G NRT++TGN+NVVDG TTFNSAT  V    F   N+T
Sbjct: 302 SGVYEENVVIAKNKRYLMMIGDGINRTVVTGNRNVVDGWTTFNSATFAVTSPNFVAVNMT 361

Query: 368 ITNTAGAIKHQAVALRVSADMTTFYSCIFEGYQDTLYAHSLRQFYRECDIYGTVDFIFGN 427
             NTAG  KHQAVA+R SAD++ FYSC FE YQDTLY HSLRQFYRECDIYGTVDFIFGN
Sbjct: 362 FRNTAGPEKHQAVAMRSSADLSIFYSCSFEAYQDTLYTHSLRQFYRECDIYGTVDFIFGN 421

Query: 428 AAVVLQNCNIYARLPLSGQFNALTAQGRTDQNQNTGIFIHKCTIKPTPDLAASP-TTKSY 487
           AAVV Q+CN+Y R P+  QFNA+TAQGRTD NQNTGI IH CTIKP  DL +S  T K+Y
Sbjct: 422 AAVVFQDCNLYPRQPMQNQFNAITAQGRTDPNQNTGISIHNCTIKPADDLVSSNYTVKTY 481

Query: 488 LGRPWKEFSRTVYMQSFIDAFIDPAGWKEWDGTLNLNTSYYAEFDNSGPGSDTSRRAQW- 547
           LGRPWKE+SRTV+MQS+ID  ++P GW+EW+G   L+T YYAE++N+G GS T+ R  W 
Sbjct: 482 LGRPWKEYSRTVFMQSYIDEVVEPVGWREWNGDFALSTLYYAEYNNTGSGSSTTDRVVWP 541

Query: 548 AVGVINATVANIFTVSEILAGDQWLPPTAVPYSGGLIS 554
              VIN+T AN FTV   L GD W+  + VPY  GL+S
Sbjct: 542 GYHVINSTDANNFTVENFLLGDGWMVQSGVPYISGLLS 573

BLAST of CmaCh20G011150 vs. Swiss-Prot
Match: PME7_ARATH (Probable pectinesterase/pectinesterase inhibitor 7 OS=Arabidopsis thaliana GN=PME7 PE=2 SV=1)

HSP 1 Score: 479.2 bits (1232), Expect = 6.2e-134
Identity = 269/580 (46.38%), Postives = 357/580 (61.55%), Query Frame = 1

Query: 6   NPISLLTFFSLLFLFLFTSSSAVPTVE-VCQSATSPSFCKSFLPSSPASVHSHCRYTLHH 65
           +PI +L   S     +  SS  +     +C++   P +CKS  P S  +V  +  +++  
Sbjct: 3   SPIFILITLSFFLQSVLASSQTLSNSSTICKTTPDPKYCKSVFPHSQGNVQQYGCFSIRK 62

Query: 66  ALVQARTFITLVDLQLTLLPSFS------ALHDCRSLAMANLDFISSTVNTVNTTT---- 125
           +L Q+R FI  VD  +      S      AL DCR LA   +D++ ++  TVN T+    
Sbjct: 63  SLSQSRKFIRTVDRYIKRNAHLSQPAVIRALQDCRFLAGLTMDYLLTSFETVNDTSAKTS 122

Query: 126 -NALPFYQADEMISLLSAVITNVDTCHDGLAGSGLGLVDKVVKA-----ISFDRQLYSLY 185
              L F +AD++ +LLSA +TN  TC +GL  +        V+      +  D +L  + 
Sbjct: 123 FKPLSFPKADDIQTLLSAALTNEQTCLEGLTTAASYSATWTVRTGVALPLVNDTKLLGVS 182

Query: 186 LSLFKMGWVSKTMKA-------PKLPKTNHFG----FGNGPLKLKMSPKDRAYYERLMHG 245
           L+LF  GWV K  K        P+   + H      F NG L LKM+ K +A YE L   
Sbjct: 183 LALFTKGWVPKKKKRAGFAWAQPRSGSSTHTKPFRLFRNGALPLKMTEKTKAVYESLSRR 242

Query: 246 KKPAARRLLQTNNADDG--ILVNGVVAVDQNGAYDFTTINDAIAAAPNKSTAANGYFLIF 305
           K           + DDG  +L++ +V V Q+G  +FT I  A+AAAPN +  + G+FLI+
Sbjct: 243 KLADGD---SNGDGDDGSMVLISDIVTVSQDGTGNFTNITAAVAAAPNNTDGSAGFFLIY 302

Query: 306 VTAGIYMESVVVPKSKKYVLMIGEGNNRTIITGNKNVVDGSTTFNSATVIVEGTGFFGAN 365
           VTAGIY E + + K+K+Y++MIG+G N+T++TGN++VVDG TTFNSAT  V    F   N
Sbjct: 303 VTAGIYEEYISIAKNKRYMMMIGDGINQTVVTGNRSVVDGWTTFNSATFAVTAPNFVAVN 362

Query: 366 LTITNTAGAIKHQAVALRVSADMTTFYSCIFEGYQDTLYAHSLRQFYRECDIYGTVDFIF 425
           +T  NTAG  KHQAVALR  AD + FYSC FE YQDTLY HSLRQFYRECD+YGTVDFIF
Sbjct: 363 ITFRNTAGPEKHQAVALRSGADFSIFYSCSFEAYQDTLYTHSLRQFYRECDVYGTVDFIF 422

Query: 426 GNAAVVLQNCNIYARLPLSGQFNALTAQGRTDQNQNTGIFIHKCTIKPTPDLAASP-TTK 485
           GNAAVV QNCN+Y R P+  QFNA+TAQGR+D NQNTG  I  CTIKP  DL +S  T K
Sbjct: 423 GNAAVVFQNCNLYPRKPMPNQFNAITAQGRSDPNQNTGTSIQNCTIKPADDLVSSNYTVK 482

Query: 486 SYLGRPWKEFSRTVYMQSFIDAFIDPAGWKEWDGTLNLNTSYYAEFDNSGPGSDTSRRAQ 545
           +YLGRPWKE+SRTVYMQS+ID F++P GW+EW+G   L+T YYAE++N+GPGS+T+ R  
Sbjct: 483 TYLGRPWKEYSRTVYMQSYIDGFVEPVGWREWNGDFALSTLYYAEYNNTGPGSNTTNRVT 542

Query: 546 W-AVGVINATVANIFTVSEILAGDQWLPPTAVPYSGGLIS 554
           W    VIN+T A  FTV+ +     W+  T VPY+ GLIS
Sbjct: 543 WPGYHVINSTDAANFTVTGLFIEADWIWKTGVPYTSGLIS 579

BLAST of CmaCh20G011150 vs. Swiss-Prot
Match: PME47_ARATH (Probable pectinesterase/pectinesterase inhibitor 47 OS=Arabidopsis thaliana GN=PME47 PE=2 SV=1)

HSP 1 Score: 416.4 bits (1069), Expect = 4.9e-115
Identity = 235/544 (43.20%), Postives = 321/544 (59.01%), Query Frame = 1

Query: 26  SAVPTVEVCQSATSPSFCKSFLP---SSPASVHSHCRYTLHHALVQARTFITLV------ 85
           S +P    C+S   P  C++ L    SSP+  + + ++T+   L QAR    ++      
Sbjct: 80  SPLPPNIACKSTPYPKLCRTILSAVKSSPSDPYHYGKFTMKQCLKQARRLSKVINRFAQR 139

Query: 86  ---DLQLTLLPSFSALHDCRSLAMANLDFISSTVNTVNTTTNALPFYQADEMISLLSAVI 145
              D   + +   SA+ DC  LA  +++++  TV         +     D + SLL  V+
Sbjct: 140 VEADPGTSTVEEVSAVADCGELAELSVEYLE-TVTEELKAAELMTAALVDRVTSLLGGVV 199

Query: 146 TNVDTCHDGLAGSGLGLVDKVVKAISFDRQLYSLYLSLFKMGWVSKTMKAPKLPKTNHFG 205
           TN  TC DGL  +  G    +   +    +LYS+ L L     +++ +K  K  K   FG
Sbjct: 200 TNQQTCLDGLVDAKSGFATAIGTPLGNLTRLYSVSLGLVSHA-LNRNLKRYKGSKGKIFG 259

Query: 206 FGNGPLKLKMSPKDRAYYERLMHGK--KPAARRLLQTNNADDG-ILVNGVVAVDQNGAYD 265
            GN P++  +    +   +    GK  + A R L +      G ILV   V V      +
Sbjct: 260 GGNKPVREPLETLIKVLRKTCDKGKDCRKANRNLGELGETSGGSILVREAVTVGPYETDN 319

Query: 266 FTTINDAIAAAPNKSTAANGYFLIFVTAGIYMESVVVPKSKKYVLMIGEGNNRTIITGNK 325
           F TI +A+AAAPN +    GYF+I+  AG+Y E VV+   K+ +++IG+G N+TII+GN 
Sbjct: 320 FPTITEAVAAAPNHTFPEQGYFVIYARAGLYEEYVVISNKKRNIMLIGDGINKTIISGNH 379

Query: 326 NVVDGSTTFNSATVIVEGTGFFGANLTITNTAGAIKHQAVALRVSADMTTFYSCIFEGYQ 385
           + +DG TT+NS+T  V G  F   ++T  NTAG  KHQAVA+R +AD +TFY C FEGYQ
Sbjct: 380 SFIDGWTTYNSSTFAVVGDRFVAVDVTFRNTAGPEKHQAVAVRNNADGSTFYRCSFEGYQ 439

Query: 386 DTLYAHSLRQFYRECDIYGTVDFIFGNAAVVLQNCNIYARLPLSGQFNALTAQGRTDQNQ 445
           DTLY HSLRQFYRECDIYGT+DFIFGNAA + QNCNIYAR P++ Q NA+TA GRTD NQ
Sbjct: 440 DTLYVHSLRQFYRECDIYGTIDFIFGNAAAIFQNCNIYARKPMANQKNAVTAHGRTDPNQ 499

Query: 446 NTGIFIHKCTIKPTPDLAASP-TTKSYLGRPWKEFSRTVYMQSFIDAFIDPAGWKEWDGT 505
            TGI I  CTI   PDLAA P +T ++LGRPWK +SRTVY+QS+I   + P GW EW+GT
Sbjct: 500 KTGISIINCTIGAAPDLAADPKSTMTFLGRPWKPYSRTVYIQSYISDVVQPVGWLEWNGT 559

Query: 506 LNLNTSYYAEFDNSGPGSDTSRRAQWA-VGVINATVANIFTVSEILAGDQWLPPTAVPYS 553
             L+T  Y E+DN GPG+DTS+R QW+   ++N   A  FTV     GD WLP T +P+ 
Sbjct: 560 TGLDTISYGEYDNFGPGADTSKRVQWSGYSLLNLVQAMNFTVYNFTLGDTWLPQTDIPFY 619

BLAST of CmaCh20G011150 vs. Swiss-Prot
Match: PME25_ARATH (Probable pectinesterase/pectinesterase inhibitor 25 OS=Arabidopsis thaliana GN=PME25 PE=2 SV=1)

HSP 1 Score: 412.5 bits (1059), Expect = 7.1e-114
Identity = 235/547 (42.96%), Postives = 326/547 (59.60%), Query Frame = 1

Query: 24  SSSAVPTVEVCQSATSPSFCKSFL---PSSPASVHSHCRYTLHHALVQARTFITLVDLQL 83
           S S  P++  C+S   P  C++ L    SSP+  + + ++T+   L QA     ++    
Sbjct: 74  SQSQSPSL-ACKSTPYPKLCRTILNAVKSSPSDPYRYGKFTIKQCLKQASRLSKVITSYA 133

Query: 84  TLLPS---------FSALHDCRSLAMANLDFISSTVNTVNTTTNALPFYQADEMISLLSA 143
             + S           A+ DC  L+  +++++  TV T   T   +     + + SLLS 
Sbjct: 134 RRVESKPGSATAEEIGAVADCGELSELSVNYLE-TVTTELKTAQVMTAALVEHVNSLLSG 193

Query: 144 VITNVDTCHDGLAGSGLGLVDKVVKAISFDRQLYSLYLSLFKMGWVSKTMKAPKLPKTNH 203
           V+TN  TC DGL  +  G    +   +    +LYS+ L L     +++ +K  K  K   
Sbjct: 194 VVTNQQTCLDGLVEAKSGFAAAIGSPMGNLTRLYSISLGLVSHA-LNRNLKRFKASKGKI 253

Query: 204 FGFGNGPLKLKMSPKDRAYYERLMHGK--KPAARRLLQTNNADDG-ILVNGVVAVDQNGA 263
            G GN   +  +    +   +   + K  +  +R L +      G ILV+  V V    +
Sbjct: 254 LGGGNSTYREPLETLIKGLRKTCDNDKDCRKTSRNLGELGETSGGSILVSKAVIVGPFKS 313

Query: 264 YDFTTINDAIAAAPNKSTAANGYFLIFVTAGIYMESVVVPKSKKYVLMIGEGNNRTIITG 323
            +FTTI DAIAAAPN +   +GYF+I+   G+Y E +VVP +KK ++++G+G N+TIITG
Sbjct: 314 DNFTTITDAIAAAPNNTRPEDGYFVIYAREGVYEEYIVVPINKKNLMLMGDGINKTIITG 373

Query: 324 NKNVVDGSTTFNSATVIVEGTGFFGANLTITNTAGAIKHQAVALRVSADMTTFYSCIFEG 383
           N NVVDG TT+N ++  V G  F   ++T  NTAG  KHQAVALR +A+ ++FY C FEG
Sbjct: 374 NHNVVDGWTTYNCSSFAVVGERFMAVDVTFRNTAGPEKHQAVALRNNAEGSSFYRCSFEG 433

Query: 384 YQDTLYAHSLRQFYRECDIYGTVDFIFGNAAVVLQNCNIYARLPLSGQFNALTAQGRTDQ 443
           YQDTLY HSLRQFYRECDIYGTVDFIFGNAA + QNCNIYAR P++ Q NA+TA GR D 
Sbjct: 434 YQDTLYVHSLRQFYRECDIYGTVDFIFGNAAAIFQNCNIYARKPMAKQKNAITAHGRLDP 493

Query: 444 NQNTGIFIHKCTIKPTPDLAASP-TTKSYLGRPWKEFSRTVYMQSFIDAFIDPAGWKEWD 503
           NQNTGI I  CTIK  PDLAA P +  ++LGRPWK +SRTV+MQS+I   + P GW EW+
Sbjct: 494 NQNTGISIINCTIKAAPDLAAEPKSAMTFLGRPWKPYSRTVFMQSYISDIVQPVGWLEWN 553

Query: 504 GTLNLNTSYYAEFDNSGPGSDTSRRAQW-AVGVINATVANIFTVSEILAGDQWLPPTAVP 554
           GT+ L+T YY E+ N GPG++T++R QW    ++N   A  FTV     GD WLP T +P
Sbjct: 554 GTIGLDTIYYGEYSNFGPGANTNQRVQWLGYNLLNLAEAMNFTVYNFTMGDTWLPQTDIP 613

BLAST of CmaCh20G011150 vs. TrEMBL
Match: M5VIT6_PRUPE (Pectinesterase OS=Prunus persica GN=PRUPE_ppa003639mg PE=4 SV=1)

HSP 1 Score: 572.8 bits (1475), Expect = 4.6e-160
Identity = 308/553 (55.70%), Postives = 382/553 (69.08%), Query Frame = 1

Query: 10  LLTFFSLLFLFLFTSSSAVPTVEVCQSATSPSFCKSFLPSSPAS--VHSHCRYTLHHALV 69
           L   FS L L    SSS V    +C+S   PSFCKS LP +  +  V+ + R+++  +L 
Sbjct: 14  LFPLFSSLSLAADNSSSPVSAGTICKSTPDPSFCKSVLPHNQTAENVYYYGRFSVRKSLS 73

Query: 70  QARTFITLVDLQLTLLPSFS-----ALHDCRSLAMANLDFISSTVNTVNTTTNALPFYQA 129
           Q+R F+ LVD  L    S S     AL DCR LA  N+DF+ ++  TVN T+  L   +A
Sbjct: 74  QSRKFLNLVDKYLKRRSSLSLTAVRALEDCRLLAGLNMDFLLNSFETVNKTSKTLSNLKA 133

Query: 130 DEMISLLSAVITNVDTCHDGLAGSGLGLVDKVVKAISFDRQLYSLYLSLFKMGWVSKTMK 189
           D++ + LSA++TN  TC DG+  +      K   +   D + YS+ L+LF  GWV K  K
Sbjct: 134 DDVQTSLSAILTNQQTCLDGIQSTASAWSLKTGLSAPNDTKFYSVSLALFTKGWVPKKKK 193

Query: 190 -APKLPKTNHFGFGNGPLKLKMSPKDRAYYERLMHGKKPAARRLLQTNNADDGILVNGVV 249
            A   P     GF NG L LKMS + RA YE +        R+LLQT   DDG+LV+ VV
Sbjct: 194 SATWKPTRKQRGFKNGRLPLKMSSRTRAVYEGV------TKRKLLQTETEDDGVLVSEVV 253

Query: 250 AVDQNGAYDFTTINDAIAAAPNKSTAANGYFLIFVTAGIYMESVVVPKSKKYVLMIGEGN 309
            V QNG  +F+TINDAIAAAPN S + NGYFLI+VTAG+Y E V + K+K+Y++M+G+G 
Sbjct: 254 TVSQNGTGNFSTINDAIAAAPNNSASTNGYFLIYVTAGVYEEYVSIAKNKRYLMMVGDGI 313

Query: 310 NRTIITGNKNVVDGSTTFNSATVIVEGTGFFGANLTITNTAGAIKHQAVALRVSADMTTF 369
           N+TI+TGN++VVDG TTFNSAT  V   GF   N+T  NTAGAIKHQAVA+R  AD++TF
Sbjct: 314 NQTILTGNRSVVDGWTTFNSATFAVTAPGFVAVNMTFRNTAGAIKHQAVAVRNGADLSTF 373

Query: 370 YSCIFEGYQDTLYAHSLRQFYRECDIYGTVDFIFGNAAVVLQNCNIYARLPLSGQFNALT 429
           YSC FE YQDTLY HSLRQFYRECDIYGTVDFIFGNAAVV QNCN+Y RLP+SGQFNA+T
Sbjct: 374 YSCSFEAYQDTLYTHSLRQFYRECDIYGTVDFIFGNAAVVFQNCNLYPRLPMSGQFNAIT 433

Query: 430 AQGRTDQNQNTGIFIHKCTIKPTPDLAAS-PTTKSYLGRPWKEFSRTVYMQSFIDAFIDP 489
           AQGRTD NQNTG  IH CTI+   DLA+S  +TK+YLGRPWKE+SRTVYMQS+ID+ +DP
Sbjct: 434 AQGRTDPNQNTGTSIHNCTIRAADDLASSNSSTKTYLGRPWKEYSRTVYMQSYIDSVVDP 493

Query: 490 AGWKEWDGTLNLNTSYYAEFDNSGPGSDTSRRAQW-AVGVINATVANIFTVSEILAGDQW 549
           AGW+ WDG   L+T YYAE++N+GPGS+T+ R  W    VINAT A  FTV+  L GD W
Sbjct: 494 AGWRAWDGEFALSTLYYAEYNNTGPGSNTTSRVTWPGYHVINATDAANFTVTNFLLGDDW 553

Query: 550 LPPTAVPYSGGLI 553
           LP T VPY+GGLI
Sbjct: 554 LPQTGVPYTGGLI 560

BLAST of CmaCh20G011150 vs. TrEMBL
Match: I1NB83_SOYBN (Pectinesterase OS=Glycine max GN=GLYMA_19G212300 PE=4 SV=1)

HSP 1 Score: 570.5 bits (1469), Expect = 2.3e-159
Identity = 307/555 (55.32%), Postives = 377/555 (67.93%), Query Frame = 1

Query: 13  FFSLLFLFLFTSSSAVPTVEV-------CQSATSPSFCKSFLPSSPASVHSHCRYTLHHA 72
           F  L+ L LF SS  VP           C+S   PSFCKS LP    +V+ + R+++  +
Sbjct: 8   FLFLILLPLFASSDTVPNPTTPVSPGTACKSTPDPSFCKSVLPPQNGNVYDYGRFSVKKS 67

Query: 73  LVQARTFITLVDLQLTLLPSFSA-----LHDCRSLAMANLDFISSTVNTVNTTTNALPFY 132
           L QAR F+ LVD  L    S SA     L DCR+L   N DF+SS+  TVN TT  LP +
Sbjct: 68  LSQARKFLNLVDKYLQRSSSLSATAIRALQDCRTLGELNFDFLSSSFQTVNKTTRFLPSF 127

Query: 133 QADEMISLLSAVITNVDTCHDGL--AGSGLGLVDKVVKAISFDRQLYSLYLSLFKMGWVS 192
           QAD++ +LLSA++TN  TC DGL    S   + + +   +S D +LYS+ L+LF  GWV 
Sbjct: 128 QADDIQTLLSAILTNQQTCLDGLKDTASAWSVRNGLTVPLSNDTKLYSVSLALFTKGWVP 187

Query: 193 KTMKAPKLPKTNHFGFGNGPLKLKMSPKDRAYYERLMHGKKPAARRLLQTNNADDGILVN 252
           +T      P     GF NG L LKMS + RA YE +      + R+LLQ    D+ ++V 
Sbjct: 188 RTKAKAMHPTKKQLGFKNGRLPLKMSSRTRAIYESV------SRRKLLQATVGDE-VVVR 247

Query: 253 GVVAVDQNGAYDFTTINDAIAAAPNKSTAANGYFLIFVTAGIYMESVVVPKSKKYVLMIG 312
            +V V Q+G+ +FTTINDAIAAAPNKS + +GYFLI+VTAG+Y E+V V K K Y++M+G
Sbjct: 248 DIVTVSQDGSGNFTTINDAIAAAPNKSVSTDGYFLIYVTAGVYEENVSVDKKKTYLMMVG 307

Query: 313 EGNNRTIITGNKNVVDGSTTFNSATVIVEGTGFFGANLTITNTAGAIKHQAVALRVSADM 372
           +G N+TIITGN++VVDG TTF+SAT+ V G GF G N+TI NTAGA+KHQAVALR  AD+
Sbjct: 308 DGINKTIITGNRSVVDGWTTFSSATLAVVGQGFVGVNMTIRNTAGAVKHQAVALRSGADL 367

Query: 373 TTFYSCIFEGYQDTLYAHSLRQFYRECDIYGTVDFIFGNAAVVLQNCNIYARLPLSGQFN 432
           +TFYSC FEGYQDTLY HSLRQFY ECDIYGTVDFIFGNA VV QNC +Y RLP+SGQFN
Sbjct: 368 STFYSCSFEGYQDTLYVHSLRQFYSECDIYGTVDFIFGNAKVVFQNCKMYPRLPMSGQFN 427

Query: 433 ALTAQGRTDQNQNTGIFIHKCTIKPTPDLAASPTTKSYLGRPWKEFSRTVYMQSFIDAFI 492
           A+TAQGRTD NQ+TGI IH CTI+   DLAAS    +YLGRPWKE+SRTVYMQ+ +D+ I
Sbjct: 428 AITAQGRTDPNQDTGISIHNCTIRAADDLAASNGVATYLGRPWKEYSRTVYMQTVMDSVI 487

Query: 493 DPAGWKEWDGTLNLNTSYYAEFDNSGPGSDTSRRAQW-AVGVINATVANIFTVSEILAGD 552
              GW+EWDG   L+T YYAE+ NSGPGS T  R  W    VINAT A  FTVS  L GD
Sbjct: 488 HAKGWREWDGDFALSTLYYAEYSNSGPGSGTDNRVTWPGYHVINATDAANFTVSNFLLGD 547

BLAST of CmaCh20G011150 vs. TrEMBL
Match: A0A0B2SGV0_GLYSO (Pectinesterase OS=Glycine soja GN=glysoja_002082 PE=4 SV=1)

HSP 1 Score: 570.5 bits (1469), Expect = 2.3e-159
Identity = 307/555 (55.32%), Postives = 377/555 (67.93%), Query Frame = 1

Query: 13  FFSLLFLFLFTSSSAVPTVEV-------CQSATSPSFCKSFLPSSPASVHSHCRYTLHHA 72
           F  L+ L LF SS  VP           C+S   PSFCKS LP    +V+ + R+++  +
Sbjct: 8   FLFLILLPLFASSDTVPNPTTPVSPGTACKSTPDPSFCKSVLPPQNGNVYDYGRFSVKKS 67

Query: 73  LVQARTFITLVDLQLTLLPSFSA-----LHDCRSLAMANLDFISSTVNTVNTTTNALPFY 132
           L QAR F+ LVD  L    S SA     L DCR+L   N DF+SS+  TVN TT  LP +
Sbjct: 68  LSQARKFLNLVDKYLQRSSSLSATAIRALQDCRTLGELNFDFLSSSFQTVNKTTRFLPSF 127

Query: 133 QADEMISLLSAVITNVDTCHDGL--AGSGLGLVDKVVKAISFDRQLYSLYLSLFKMGWVS 192
           QAD++ +LLSA++TN  TC DGL    S   + + +   +S D +LYS+ L+LF  GWV 
Sbjct: 128 QADDIQTLLSAILTNQQTCLDGLKDTASAWSVRNGLTVPLSNDTKLYSVSLALFTKGWVP 187

Query: 193 KTMKAPKLPKTNHFGFGNGPLKLKMSPKDRAYYERLMHGKKPAARRLLQTNNADDGILVN 252
           +T      P     GF NG L LKMS + RA YE +      + R+LLQ    D+ ++V 
Sbjct: 188 RTKAKAMHPTKKQLGFKNGRLPLKMSSRTRAIYESV------SRRKLLQATVGDE-VVVR 247

Query: 253 GVVAVDQNGAYDFTTINDAIAAAPNKSTAANGYFLIFVTAGIYMESVVVPKSKKYVLMIG 312
            +V V Q+G+ +FTTINDAIAAAPNKS + +GYFLI+VTAG+Y E+V V K K Y++M+G
Sbjct: 248 DIVTVSQDGSGNFTTINDAIAAAPNKSVSTDGYFLIYVTAGVYEENVSVDKKKTYLMMVG 307

Query: 313 EGNNRTIITGNKNVVDGSTTFNSATVIVEGTGFFGANLTITNTAGAIKHQAVALRVSADM 372
           +G N+TIITGN++VVDG TTF+SAT+ V G GF G N+TI NTAGA+KHQAVALR  AD+
Sbjct: 308 DGINKTIITGNRSVVDGWTTFSSATLAVVGQGFVGVNMTIRNTAGAVKHQAVALRSGADL 367

Query: 373 TTFYSCIFEGYQDTLYAHSLRQFYRECDIYGTVDFIFGNAAVVLQNCNIYARLPLSGQFN 432
           +TFYSC FEGYQDTLY HSLRQFY ECDIYGTVDFIFGNA VV QNC +Y RLP+SGQFN
Sbjct: 368 STFYSCSFEGYQDTLYVHSLRQFYSECDIYGTVDFIFGNAKVVFQNCKMYPRLPMSGQFN 427

Query: 433 ALTAQGRTDQNQNTGIFIHKCTIKPTPDLAASPTTKSYLGRPWKEFSRTVYMQSFIDAFI 492
           A+TAQGRTD NQ+TGI IH CTI+   DLAAS    +YLGRPWKE+SRTVYMQ+ +D+ I
Sbjct: 428 AITAQGRTDPNQDTGISIHNCTIRAADDLAASNGVATYLGRPWKEYSRTVYMQTVMDSVI 487

Query: 493 DPAGWKEWDGTLNLNTSYYAEFDNSGPGSDTSRRAQW-AVGVINATVANIFTVSEILAGD 552
              GW+EWDG   L+T YYAE+ NSGPGS T  R  W    VINAT A  FTVS  L GD
Sbjct: 488 HAKGWREWDGDFALSTLYYAEYSNSGPGSGTDNRVTWPGYHVINATDAANFTVSNFLLGD 547

BLAST of CmaCh20G011150 vs. TrEMBL
Match: M5XRB3_PRUPE (Pectinesterase OS=Prunus persica GN=PRUPE_ppa003581mg PE=4 SV=1)

HSP 1 Score: 565.8 bits (1457), Expect = 5.6e-158
Identity = 309/569 (54.31%), Postives = 387/569 (68.01%), Query Frame = 1

Query: 4   SSNPISLLTFFSLLFLFLFTSSSAV---------PTVEVCQSATSPSFCKSFLPSSPASV 63
           +S   SL TF S L +F   SS ++         P   +C+S   PS+C S LP   A+V
Sbjct: 2   ASKLFSLATFSSFLIIFHSLSSPSLADVPLDTPLPPETICKSTPHPSYCISVLPHKNANV 61

Query: 64  HSHCRYTLHHALVQARTFITLVDLQLTL-----LPSFSALHDCRSLAMANLDFISSTVNT 123
           +   R+++ HAL Q+      ++  L L      P+  AL DC+ L + N+DF+SS++ T
Sbjct: 62  YDFGRFSVQHALSQSHNLFDSIENHLQLGSVLPQPAIQALEDCKLLTLLNIDFLSSSLET 121

Query: 124 VNTTTNALPFYQADEMISLLSAVITNVDTCHDGLAG---SGLGLVDKVVKAISFDRQLYS 183
           VN T+  L    AD++ +LLSA++TN  TC DGL     S   L++ +  +IS + QLYS
Sbjct: 122 VNKTSGVLASLDADDVQTLLSAILTNQQTCSDGLESLPSSAGSLINDLSASISNNSQLYS 181

Query: 184 LYLSLFKMGWVSKTMKA-PKLPKTNHFGFGNGPLKLKMSPKDRAYYERLMHGKKPAARRL 243
           + L+LF  GWV K     PK PK  H  FG G L LKMS + RA Y+  ++ ++   RRL
Sbjct: 182 VSLALFTKGWVPKDKNGVPKQPK-RHLQFGKGRLNLKMSSQARAIYDAAINHRR---RRL 241

Query: 244 LQTNNADDGILVNGVVAVDQNGAYDFTTINDAIAAAPNKSTAANGYFLIFVTAGIYMESV 303
           LQ    D+ +LV G+V V Q+G+ +FTTIN AIAAAPN S A+ GYFLI+VTAG+Y E V
Sbjct: 242 LQVG--DEEVLVKGIVVVSQDGSGNFTTINAAIAAAPNNSVASGGYFLIYVTAGVYQEYV 301

Query: 304 VVPKSKKYVLMIGEGNNRTIITGNKNVVDGSTTFNSATVIVEGTGFFGANLTITNTAGAI 363
            +  +KKY+LMIG+G N+TIITGN +V DGSTTFNSAT+ V G GF   N+T+ NTAG  
Sbjct: 302 SIASNKKYLLMIGDGINQTIITGNNSVGDGSTTFNSATLAVTGLGFVAVNITVRNTAGPS 361

Query: 364 KHQAVALRVSADMTTFYSCIFEGYQDTLYAHSLRQFYRECDIYGTVDFIFGNAAVVLQNC 423
           K QAVALR  AD++ FYSC FEGYQDTLY HSLRQFYRECDIYGTVDFIFGNAAVV QNC
Sbjct: 362 KGQAVALRSGADLSVFYSCSFEGYQDTLYTHSLRQFYRECDIYGTVDFIFGNAAVVFQNC 421

Query: 424 NIYARLPLSGQFNALTAQGRTDQNQNTGIFIHKCTIKPTPDLAASP-TTKSYLGRPWKEF 483
           N+Y R P  GQ NA+TAQGRTD NQNTG  IH CTIKPTPDLA+S  T K+YLGRPWKE+
Sbjct: 422 NLYPRQPNKGQSNAITAQGRTDPNQNTGTSIHNCTIKPTPDLASSNFTVKTYLGRPWKEY 481

Query: 484 SRTVYMQSFIDAFIDPAGWKEWDGTLNLNTSYYAEFDNSGPGSDTSRRAQW-AVGVINAT 543
           SRTVYMQ+F+ + IDPAGW  W G   L+T YYAE++N+GPGS+T+ R  W    VIN+T
Sbjct: 482 SRTVYMQTFMASLIDPAGWLAWSGDFALSTLYYAEYNNTGPGSNTTNRVTWPGYHVINST 541

Query: 544 VANIFTVSEILAGDQWLPPTAVPYSGGLI 553
           VA  FTV+  L GD WL  T VPY+GGLI
Sbjct: 542 VAANFTVTNFLMGDNWLAATGVPYTGGLI 564

BLAST of CmaCh20G011150 vs. TrEMBL
Match: A0A0B2QJL1_GLYSO (Pectinesterase OS=Glycine soja GN=glysoja_039687 PE=4 SV=1)

HSP 1 Score: 564.3 bits (1453), Expect = 1.6e-157
Identity = 304/556 (54.68%), Postives = 381/556 (68.53%), Query Frame = 1

Query: 10  LLTFFSLLFLFLFTSSSAVPTVEV-----CQSATSPSFCKSFLPSSPASVHSHCRYTLHH 69
           L+ F  LL LF  + +   PT  V     C+S   PS+CKS LP    +V+ + R+++  
Sbjct: 8   LILFLILLPLFASSETVPNPTTSVSPGTACKSTPDPSYCKSVLPPQNGNVYDYGRFSVKK 67

Query: 70  ALVQARTFITLVDLQLTLLPSFSA-----LHDCRSLAMANLDFISSTVNTVNTTTNALPF 129
           +L QAR F+ LVD  L    S SA     L DCR+L   N DF+SS+  TVN TT  LP 
Sbjct: 68  SLSQARKFLNLVDKYLQRGSSLSATAIRALQDCRTLGELNFDFLSSSFQTVNKTTRFLPS 127

Query: 130 YQADEMISLLSAVITNVDTCHDGL--AGSGLGLVDKVVKAISFDRQLYSLYLSLFKMGWV 189
           +QAD++ +LLSA++TN  TC DGL    S   + + +   +S D +LYS+ L+LF  GWV
Sbjct: 128 FQADDIQTLLSAILTNQQTCLDGLKDTASAWSVRNGLSVPLSNDTKLYSVSLALFIKGWV 187

Query: 190 SKTMKAPKLPKTNHFGFGNGPLKLKMSPKDRAYYERLMHGKKPAARRLLQTNNADDGILV 249
            +T      P     GF NG L LKMS + RA YE +      + R+LLQ    D+ ++V
Sbjct: 188 PRTKAKAMHPTKKQLGFKNGRLPLKMSSRTRAIYESV------SRRKLLQAKVGDE-VVV 247

Query: 250 NGVVAVDQNGAYDFTTINDAIAAAPNKSTAANGYFLIFVTAGIYMESVVVPKSKKYVLMI 309
             +V V Q+G+ +FTTINDAIAAAPNKS + +GYFLI+VTAG+Y E+V + K K Y++M+
Sbjct: 248 RDIVTVSQDGSGNFTTINDAIAAAPNKSVSTDGYFLIYVTAGVYEENVSIDKKKTYLMMV 307

Query: 310 GEGNNRTIITGNKNVVDGSTTFNSATVIVEGTGFFGANLTITNTAGAIKHQAVALRVSAD 369
           G+G N+TIITGN++VVDG TTF+SAT+ V G GF G N+TI NTAGA+KHQAVALR  AD
Sbjct: 308 GDGINKTIITGNRSVVDGWTTFSSATLAVVGQGFVGVNMTIRNTAGAVKHQAVALRSGAD 367

Query: 370 MTTFYSCIFEGYQDTLYAHSLRQFYRECDIYGTVDFIFGNAAVVLQNCNIYARLPLSGQF 429
           ++TFYSC FEGYQDTLY HSLRQFY ECDI+GTVDFIFGNA VV QNCN+Y RLP+SGQF
Sbjct: 368 LSTFYSCSFEGYQDTLYVHSLRQFYSECDIFGTVDFIFGNAKVVFQNCNMYPRLPMSGQF 427

Query: 430 NALTAQGRTDQNQNTGIFIHKCTIKPTPDLAASPTTKSYLGRPWKEFSRTVYMQSFIDAF 489
           NA+TAQGRTD NQ+TGI IH  TI+   DLA+S    +YLGRPWKE+SRTVYMQ+F+D+ 
Sbjct: 428 NAITAQGRTDPNQDTGISIHNSTIRAADDLASSNGVATYLGRPWKEYSRTVYMQTFMDSV 487

Query: 490 IDPAGWKEWDGTLNLNTSYYAEFDNSGPGSDTSRRAQW-AVGVINATVANIFTVSEILAG 549
           I   GW+EWDG   L+T YYAE+ NSGPGS T  R  W    VINAT A+ FTVS  L G
Sbjct: 488 IHAKGWREWDGDFALSTLYYAEYSNSGPGSGTDNRVTWPGYHVINATDASNFTVSNFLLG 547

Query: 550 DQWLPPTAVPYSGGLI 553
           D WLP T V Y+  LI
Sbjct: 548 DDWLPQTGVSYTNNLI 556

BLAST of CmaCh20G011150 vs. TAIR10
Match: AT2G47550.1 (AT2G47550.1 Plant invertase/pectin methylesterase inhibitor superfamily)

HSP 1 Score: 518.5 bits (1334), Expect = 5.2e-147
Identity = 283/569 (49.74%), Postives = 372/569 (65.38%), Query Frame = 1

Query: 16  LLFLFLFTSSSAVPTV----------------EVCQSATSPSFCKSFLPSSPASVHSHCR 75
           L+FLF     S++P+                  +C+ A  PS+C+S LP+ P  ++S+ R
Sbjct: 5   LMFLFTLACLSSLPSPFISAQIPAIGNATSPSNICRFAPDPSYCRSVLPNQPGDIYSYGR 64

Query: 76  YTLHHALVQARTFITLVDLQL------TLLPSFSALHDCRSLAMANLDFISSTVNTVNTT 135
            +L  +L +AR FI+++D +L          +  AL DC+ LA   +D++ S+  T ++T
Sbjct: 65  LSLRRSLSRARRFISMIDAELDRKGKVAAKSTVGALEDCKFLASLTMDYLLSSSQTADST 124

Query: 136 TNALPFYQADEMISLLSAVITNVDTCHDGL--AGSGLGLVDKVVKAISFDRQLYSLYLSL 195
              L   +A+++ + LSA ITN  TC +GL    S  GL   +      D +LY + L+L
Sbjct: 125 -KTLSLSRAEDVHTFLSAAITNEQTCLEGLKSTASENGLSGDLFN----DTKLYGVSLAL 184

Query: 196 FKMGWVSKTMKA-----PKLPKTNHFGFGNGPLKLKMSPKDRAYYERLMHGKKPAARRLL 255
           F  GWV +  ++     P+      FGF NG L LKM+ + RA Y  +        R+LL
Sbjct: 185 FSKGWVPRRQRSRPIWQPQARFKKFFGFRNGKLPLKMTERARAVYNTVTR------RKLL 244

Query: 256 QTNNADDGILVNGVVAVDQNGAYDFTTINDAIAAAPNKSTAANGYFLIFVTAGIYMESVV 315
           Q++   D + V+ +V V QNG  +FTTIN AIAAAPNK+  +NGYFLI+VTAG+Y E V 
Sbjct: 245 QSDA--DAVQVSDIVTVIQNGTGNFTTINAAIAAAPNKTDGSNGYFLIYVTAGLYEEYVE 304

Query: 316 VPKSKKYVLMIGEGNNRTIITGNKNVVDGSTTFNSATVIVEGTGFFGANLTITNTAGAIK 375
           VPK+K+YV+MIG+G N+T+ITGN++VVDG TTFNSAT I+ G  F G N+TI NTAG  K
Sbjct: 305 VPKNKRYVMMIGDGINQTVITGNRSVVDGWTTFNSATFILSGPNFIGVNITIRNTAGPTK 364

Query: 376 HQAVALRVSADMTTFYSCIFEGYQDTLYAHSLRQFYRECDIYGTVDFIFGNAAVVLQNCN 435
            QAVALR   D++ FYSC FE YQDTLY HSLRQFYRECD+YGTVDFIFGNAAVVLQNCN
Sbjct: 365 GQAVALRSGGDLSVFYSCSFEAYQDTLYTHSLRQFYRECDVYGTVDFIFGNAAVVLQNCN 424

Query: 436 IYARLPLSGQFNALTAQGRTDQNQNTGIFIHKCTIKPTPDLAASP-TTKSYLGRPWKEFS 495
           +Y R P  GQ N +TAQGRTD NQNTG  IH CTI+P  DLA S  T K+YLGRPWKE+S
Sbjct: 425 LYPRQPRKGQSNEVTAQGRTDPNQNTGTAIHGCTIRPADDLATSNYTVKTYLGRPWKEYS 484

Query: 496 RTVYMQSFIDAFIDPAGWKEWDGTLNLNTSYYAEFDNSGPGSDTSRRAQW-AVGVINATV 554
           RTV MQ++ID F++P+GW  W G   L+T YYAE++N+GPGSDT+ R  W    VINAT 
Sbjct: 485 RTVVMQTYIDGFLEPSGWNAWSGDFALSTLYYAEYNNTGPGSDTTNRVTWPGYHVINATD 544

BLAST of CmaCh20G011150 vs. TAIR10
Match: AT4G02330.1 (AT4G02330.1 Plant invertase/pectin methylesterase inhibitor superfamily)

HSP 1 Score: 509.2 bits (1310), Expect = 3.1e-144
Identity = 287/578 (49.65%), Postives = 372/578 (64.36%), Query Frame = 1

Query: 8   ISLLTFFSLLFLFLFTSSSAVPTV-------EVCQSATSPSFCKSFLP-SSPASVHSHCR 67
           +SL  F   LFL L T   A  T+        +C++   P FCKS  P +S   V  + R
Sbjct: 2   LSLKLFLVTLFLSLQTLFIASQTLLPSNSSSTICKTTPDPKFCKSVFPQTSQGDVREYGR 61

Query: 68  YTLHHALVQARTFITLVDLQL----TLLP--SFSALHDCRSLAMANLDFISSTVNTVNTT 127
           ++L  +L Q+R F   +D  L     LL   +  AL DCR LA    D++ ++  TVN T
Sbjct: 62  FSLRKSLTQSRKFTRTIDRYLKRNNALLSQSAVGALQDCRYLASLTTDYLITSFETVNIT 121

Query: 128 TNA--LPFYQADEMISLLSAVITNVDTCHDGL---AGSGLGLVDKVVKAISFDRQLYSLY 187
           T++  L F +ADE+ +LLSA +TN  TC DG+   A S   + + V   +  D +L+S+ 
Sbjct: 122 TSSKTLSFSKADEIQTLLSAALTNEQTCLDGINTAASSSWTIRNGVALPLINDTKLFSVS 181

Query: 188 LSLFKMGWVSKTMKAPKL-----PKTNHFG------FGNGPLKLKMSPKDRAYYERLMHG 247
           L+LF  GWV K  K         PK  H        F NG L LKM+   RA YE L   
Sbjct: 182 LALFTKGWVPKKKKQVASYSWAHPKNTHSHTKPFRHFRNGALPLKMTEHTRAVYESL--- 241

Query: 248 KKPAARRLLQTNNADDGILVNGVVAVDQNGAYDFTTINDAIAAAPNKSTAANGYFLIFVT 307
              + R+L   +N  + +LV+ +V V+QNG  +FTTI +A+ +APNK+    GYF+I+VT
Sbjct: 242 ---SRRKLADDDNDVNTVLVSDIVTVNQNGTGNFTTITEAVNSAPNKTDGTAGYFVIYVT 301

Query: 308 AGIYMESVVVPKSKKYVLMIGEGNNRTIITGNKNVVDGSTTFNSATVIVEGTGFFGANLT 367
           +G+Y E+VV+ K+K+Y++MIG+G NRT++TGN+NVVDG TTFNSAT  V    F   N+T
Sbjct: 302 SGVYEENVVIAKNKRYLMMIGDGINRTVVTGNRNVVDGWTTFNSATFAVTSPNFVAVNMT 361

Query: 368 ITNTAGAIKHQAVALRVSADMTTFYSCIFEGYQDTLYAHSLRQFYRECDIYGTVDFIFGN 427
             NTAG  KHQAVA+R SAD++ FYSC FE YQDTLY HSLRQFYRECDIYGTVDFIFGN
Sbjct: 362 FRNTAGPEKHQAVAMRSSADLSIFYSCSFEAYQDTLYTHSLRQFYRECDIYGTVDFIFGN 421

Query: 428 AAVVLQNCNIYARLPLSGQFNALTAQGRTDQNQNTGIFIHKCTIKPTPDLAASP-TTKSY 487
           AAVV Q+CN+Y R P+  QFNA+TAQGRTD NQNTGI IH CTIKP  DL +S  T K+Y
Sbjct: 422 AAVVFQDCNLYPRQPMQNQFNAITAQGRTDPNQNTGISIHNCTIKPADDLVSSNYTVKTY 481

Query: 488 LGRPWKEFSRTVYMQSFIDAFIDPAGWKEWDGTLNLNTSYYAEFDNSGPGSDTSRRAQW- 547
           LGRPWKE+SRTV+MQS+ID  ++P GW+EW+G   L+T YYAE++N+G GS T+ R  W 
Sbjct: 482 LGRPWKEYSRTVFMQSYIDEVVEPVGWREWNGDFALSTLYYAEYNNTGSGSSTTDRVVWP 541

Query: 548 AVGVINATVANIFTVSEILAGDQWLPPTAVPYSGGLIS 554
              VIN+T AN FTV   L GD W+  + VPY  GL+S
Sbjct: 542 GYHVINSTDANNFTVENFLLGDGWMVQSGVPYISGLLS 573

BLAST of CmaCh20G011150 vs. TAIR10
Match: AT1G02810.1 (AT1G02810.1 Plant invertase/pectin methylesterase inhibitor superfamily)

HSP 1 Score: 479.2 bits (1232), Expect = 3.5e-135
Identity = 269/580 (46.38%), Postives = 357/580 (61.55%), Query Frame = 1

Query: 6   NPISLLTFFSLLFLFLFTSSSAVPTVE-VCQSATSPSFCKSFLPSSPASVHSHCRYTLHH 65
           +PI +L   S     +  SS  +     +C++   P +CKS  P S  +V  +  +++  
Sbjct: 3   SPIFILITLSFFLQSVLASSQTLSNSSTICKTTPDPKYCKSVFPHSQGNVQQYGCFSIRK 62

Query: 66  ALVQARTFITLVDLQLTLLPSFS------ALHDCRSLAMANLDFISSTVNTVNTTT---- 125
           +L Q+R FI  VD  +      S      AL DCR LA   +D++ ++  TVN T+    
Sbjct: 63  SLSQSRKFIRTVDRYIKRNAHLSQPAVIRALQDCRFLAGLTMDYLLTSFETVNDTSAKTS 122

Query: 126 -NALPFYQADEMISLLSAVITNVDTCHDGLAGSGLGLVDKVVKA-----ISFDRQLYSLY 185
              L F +AD++ +LLSA +TN  TC +GL  +        V+      +  D +L  + 
Sbjct: 123 FKPLSFPKADDIQTLLSAALTNEQTCLEGLTTAASYSATWTVRTGVALPLVNDTKLLGVS 182

Query: 186 LSLFKMGWVSKTMKA-------PKLPKTNHFG----FGNGPLKLKMSPKDRAYYERLMHG 245
           L+LF  GWV K  K        P+   + H      F NG L LKM+ K +A YE L   
Sbjct: 183 LALFTKGWVPKKKKRAGFAWAQPRSGSSTHTKPFRLFRNGALPLKMTEKTKAVYESLSRR 242

Query: 246 KKPAARRLLQTNNADDG--ILVNGVVAVDQNGAYDFTTINDAIAAAPNKSTAANGYFLIF 305
           K           + DDG  +L++ +V V Q+G  +FT I  A+AAAPN +  + G+FLI+
Sbjct: 243 KLADGD---SNGDGDDGSMVLISDIVTVSQDGTGNFTNITAAVAAAPNNTDGSAGFFLIY 302

Query: 306 VTAGIYMESVVVPKSKKYVLMIGEGNNRTIITGNKNVVDGSTTFNSATVIVEGTGFFGAN 365
           VTAGIY E + + K+K+Y++MIG+G N+T++TGN++VVDG TTFNSAT  V    F   N
Sbjct: 303 VTAGIYEEYISIAKNKRYMMMIGDGINQTVVTGNRSVVDGWTTFNSATFAVTAPNFVAVN 362

Query: 366 LTITNTAGAIKHQAVALRVSADMTTFYSCIFEGYQDTLYAHSLRQFYRECDIYGTVDFIF 425
           +T  NTAG  KHQAVALR  AD + FYSC FE YQDTLY HSLRQFYRECD+YGTVDFIF
Sbjct: 363 ITFRNTAGPEKHQAVALRSGADFSIFYSCSFEAYQDTLYTHSLRQFYRECDVYGTVDFIF 422

Query: 426 GNAAVVLQNCNIYARLPLSGQFNALTAQGRTDQNQNTGIFIHKCTIKPTPDLAASP-TTK 485
           GNAAVV QNCN+Y R P+  QFNA+TAQGR+D NQNTG  I  CTIKP  DL +S  T K
Sbjct: 423 GNAAVVFQNCNLYPRKPMPNQFNAITAQGRSDPNQNTGTSIQNCTIKPADDLVSSNYTVK 482

Query: 486 SYLGRPWKEFSRTVYMQSFIDAFIDPAGWKEWDGTLNLNTSYYAEFDNSGPGSDTSRRAQ 545
           +YLGRPWKE+SRTVYMQS+ID F++P GW+EW+G   L+T YYAE++N+GPGS+T+ R  
Sbjct: 483 TYLGRPWKEYSRTVYMQSYIDGFVEPVGWREWNGDFALSTLYYAEYNNTGPGSNTTNRVT 542

Query: 546 W-AVGVINATVANIFTVSEILAGDQWLPPTAVPYSGGLIS 554
           W    VIN+T A  FTV+ +     W+  T VPY+ GLIS
Sbjct: 543 WPGYHVINSTDAANFTVTGLFIEADWIWKTGVPYTSGLIS 579

BLAST of CmaCh20G011150 vs. TAIR10
Match: AT5G04970.1 (AT5G04970.1 Plant invertase/pectin methylesterase inhibitor superfamily)

HSP 1 Score: 416.4 bits (1069), Expect = 2.8e-116
Identity = 235/544 (43.20%), Postives = 321/544 (59.01%), Query Frame = 1

Query: 26  SAVPTVEVCQSATSPSFCKSFLP---SSPASVHSHCRYTLHHALVQARTFITLV------ 85
           S +P    C+S   P  C++ L    SSP+  + + ++T+   L QAR    ++      
Sbjct: 80  SPLPPNIACKSTPYPKLCRTILSAVKSSPSDPYHYGKFTMKQCLKQARRLSKVINRFAQR 139

Query: 86  ---DLQLTLLPSFSALHDCRSLAMANLDFISSTVNTVNTTTNALPFYQADEMISLLSAVI 145
              D   + +   SA+ DC  LA  +++++  TV         +     D + SLL  V+
Sbjct: 140 VEADPGTSTVEEVSAVADCGELAELSVEYLE-TVTEELKAAELMTAALVDRVTSLLGGVV 199

Query: 146 TNVDTCHDGLAGSGLGLVDKVVKAISFDRQLYSLYLSLFKMGWVSKTMKAPKLPKTNHFG 205
           TN  TC DGL  +  G    +   +    +LYS+ L L     +++ +K  K  K   FG
Sbjct: 200 TNQQTCLDGLVDAKSGFATAIGTPLGNLTRLYSVSLGLVSHA-LNRNLKRYKGSKGKIFG 259

Query: 206 FGNGPLKLKMSPKDRAYYERLMHGK--KPAARRLLQTNNADDG-ILVNGVVAVDQNGAYD 265
            GN P++  +    +   +    GK  + A R L +      G ILV   V V      +
Sbjct: 260 GGNKPVREPLETLIKVLRKTCDKGKDCRKANRNLGELGETSGGSILVREAVTVGPYETDN 319

Query: 266 FTTINDAIAAAPNKSTAANGYFLIFVTAGIYMESVVVPKSKKYVLMIGEGNNRTIITGNK 325
           F TI +A+AAAPN +    GYF+I+  AG+Y E VV+   K+ +++IG+G N+TII+GN 
Sbjct: 320 FPTITEAVAAAPNHTFPEQGYFVIYARAGLYEEYVVISNKKRNIMLIGDGINKTIISGNH 379

Query: 326 NVVDGSTTFNSATVIVEGTGFFGANLTITNTAGAIKHQAVALRVSADMTTFYSCIFEGYQ 385
           + +DG TT+NS+T  V G  F   ++T  NTAG  KHQAVA+R +AD +TFY C FEGYQ
Sbjct: 380 SFIDGWTTYNSSTFAVVGDRFVAVDVTFRNTAGPEKHQAVAVRNNADGSTFYRCSFEGYQ 439

Query: 386 DTLYAHSLRQFYRECDIYGTVDFIFGNAAVVLQNCNIYARLPLSGQFNALTAQGRTDQNQ 445
           DTLY HSLRQFYRECDIYGT+DFIFGNAA + QNCNIYAR P++ Q NA+TA GRTD NQ
Sbjct: 440 DTLYVHSLRQFYRECDIYGTIDFIFGNAAAIFQNCNIYARKPMANQKNAVTAHGRTDPNQ 499

Query: 446 NTGIFIHKCTIKPTPDLAASP-TTKSYLGRPWKEFSRTVYMQSFIDAFIDPAGWKEWDGT 505
            TGI I  CTI   PDLAA P +T ++LGRPWK +SRTVY+QS+I   + P GW EW+GT
Sbjct: 500 KTGISIINCTIGAAPDLAADPKSTMTFLGRPWKPYSRTVYIQSYISDVVQPVGWLEWNGT 559

Query: 506 LNLNTSYYAEFDNSGPGSDTSRRAQWA-VGVINATVANIFTVSEILAGDQWLPPTAVPYS 553
             L+T  Y E+DN GPG+DTS+R QW+   ++N   A  FTV     GD WLP T +P+ 
Sbjct: 560 TGLDTISYGEYDNFGPGADTSKRVQWSGYSLLNLVQAMNFTVYNFTLGDTWLPQTDIPFY 619

BLAST of CmaCh20G011150 vs. TAIR10
Match: AT3G10720.2 (AT3G10720.2 Plant invertase/pectin methylesterase inhibitor superfamily)

HSP 1 Score: 412.5 bits (1059), Expect = 4.0e-115
Identity = 235/547 (42.96%), Postives = 326/547 (59.60%), Query Frame = 1

Query: 24  SSSAVPTVEVCQSATSPSFCKSFL---PSSPASVHSHCRYTLHHALVQARTFITLVDLQL 83
           S S  P++  C+S   P  C++ L    SSP+  + + ++T+   L QA     ++    
Sbjct: 74  SQSQSPSL-ACKSTPYPKLCRTILNAVKSSPSDPYRYGKFTIKQCLKQASRLSKVITSYA 133

Query: 84  TLLPS---------FSALHDCRSLAMANLDFISSTVNTVNTTTNALPFYQADEMISLLSA 143
             + S           A+ DC  L+  +++++  TV T   T   +     + + SLLS 
Sbjct: 134 RRVESKPGSATAEEIGAVADCGELSELSVNYLE-TVTTELKTAQVMTAALVEHVNSLLSG 193

Query: 144 VITNVDTCHDGLAGSGLGLVDKVVKAISFDRQLYSLYLSLFKMGWVSKTMKAPKLPKTNH 203
           V+TN  TC DGL  +  G    +   +    +LYS+ L L     +++ +K  K  K   
Sbjct: 194 VVTNQQTCLDGLVEAKSGFAAAIGSPMGNLTRLYSISLGLVSHA-LNRNLKRFKASKGKI 253

Query: 204 FGFGNGPLKLKMSPKDRAYYERLMHGK--KPAARRLLQTNNADDG-ILVNGVVAVDQNGA 263
            G GN   +  +    +   +   + K  +  +R L +      G ILV+  V V    +
Sbjct: 254 LGGGNSTYREPLETLIKGLRKTCDNDKDCRKTSRNLGELGETSGGSILVSKAVIVGPFKS 313

Query: 264 YDFTTINDAIAAAPNKSTAANGYFLIFVTAGIYMESVVVPKSKKYVLMIGEGNNRTIITG 323
            +FTTI DAIAAAPN +   +GYF+I+   G+Y E +VVP +KK ++++G+G N+TIITG
Sbjct: 314 DNFTTITDAIAAAPNNTRPEDGYFVIYAREGVYEEYIVVPINKKNLMLMGDGINKTIITG 373

Query: 324 NKNVVDGSTTFNSATVIVEGTGFFGANLTITNTAGAIKHQAVALRVSADMTTFYSCIFEG 383
           N NVVDG TT+N ++  V G  F   ++T  NTAG  KHQAVALR +A+ ++FY C FEG
Sbjct: 374 NHNVVDGWTTYNCSSFAVVGERFMAVDVTFRNTAGPEKHQAVALRNNAEGSSFYRCSFEG 433

Query: 384 YQDTLYAHSLRQFYRECDIYGTVDFIFGNAAVVLQNCNIYARLPLSGQFNALTAQGRTDQ 443
           YQDTLY HSLRQFYRECDIYGTVDFIFGNAA + QNCNIYAR P++ Q NA+TA GR D 
Sbjct: 434 YQDTLYVHSLRQFYRECDIYGTVDFIFGNAAAIFQNCNIYARKPMAKQKNAITAHGRLDP 493

Query: 444 NQNTGIFIHKCTIKPTPDLAASP-TTKSYLGRPWKEFSRTVYMQSFIDAFIDPAGWKEWD 503
           NQNTGI I  CTIK  PDLAA P +  ++LGRPWK +SRTV+MQS+I   + P GW EW+
Sbjct: 494 NQNTGISIINCTIKAAPDLAAEPKSAMTFLGRPWKPYSRTVFMQSYISDIVQPVGWLEWN 553

Query: 504 GTLNLNTSYYAEFDNSGPGSDTSRRAQW-AVGVINATVANIFTVSEILAGDQWLPPTAVP 554
           GT+ L+T YY E+ N GPG++T++R QW    ++N   A  FTV     GD WLP T +P
Sbjct: 554 GTIGLDTIYYGEYSNFGPGANTNQRVQWLGYNLLNLAEAMNFTVYNFTMGDTWLPQTDIP 613

BLAST of CmaCh20G011150 vs. NCBI nr
Match: gi|659116086|ref|XP_008457895.1| (PREDICTED: pectinesterase-like [Cucumis melo])

HSP 1 Score: 872.5 bits (2253), Expect = 4.0e-250
Identity = 436/554 (78.70%), Postives = 482/554 (87.00%), Query Frame = 1

Query: 4   SSNPISLLTFFSLLFLFLFTSSSAVPTVEVCQSATSPSFCKSFLPSSPASVHSHCRYTLH 63
           SSNPIS+LTF +LLF FL  SSSAVPT E+CQS TSP FC  FLP+SPASVHSHCR+TLH
Sbjct: 3   SSNPISVLTFLTLLF-FLSPSSSAVPTAEICQSTTSPPFCNFFLPNSPASVHSHCRFTLH 62

Query: 64  HALVQARTFITLVDLQLTLLPSFSALHDCRSLAMANLDFISSTVNTVN-TTTNALPFYQA 123
           HALV ARTF++LV+  L L PS S LHDCRSLA ANLDF+S T + VN TTT  LP+Y A
Sbjct: 63  HALVHARTFLSLVNAHLNLFPSLSPLHDCRSLAEANLDFLSQTFSIVNSTTTTLLPYYDA 122

Query: 124 DEMISLLSAVITNVDTCHDGLAG--SGLGLVDKVVKAISFDRQLYSLYLSLFKMGWVSKT 183
            E ++L+SAVITN DTC++GLAG  S +GLVDKV++AISFD++LYSLYLSLFKMGWVSK 
Sbjct: 123 HEKLTLMSAVITNEDTCYEGLAGLDSAVGLVDKVLEAISFDKKLYSLYLSLFKMGWVSKD 182

Query: 184 MKAPKLPKTNHFGFGNGPLKLKMSPKDRAYYERLMH-GKKPAARRLLQTNNADDGILVNG 243
           +KAP LPK  HFG G G LKLKMSPKDRAYYERL+H  K P ARRLLQTN  DDGI++NG
Sbjct: 183 LKAPTLPKMKHFGVGKGQLKLKMSPKDRAYYERLVHRNKPPGARRLLQTNYQDDGIVING 242

Query: 244 VVAVDQNGAYDFTTINDAIAAAPNKSTAANGYFLIFVTAGIYMESVVVPKSKKYVLMIGE 303
           +V VDQNG YDFT I  AIAAAPNK+T A GYFLIFV AGIY E+V++PK K+YVL+IGE
Sbjct: 243 IVGVDQNGTYDFTNITAAIAAAPNKTTVAKGYFLIFVAAGIYNETVLIPKEKRYVLLIGE 302

Query: 304 GNNRTIITGNKNVVDGSTTFNSATVIVEGTGFFGANLTITNTAGAIKHQAVALRVSADMT 363
           GNN+TIITGNKNVVDGSTTFNSATV VEGTGF G NLTI+NTAGA KHQAVALRVSAD  
Sbjct: 303 GNNQTIITGNKNVVDGSTTFNSATVAVEGTGFLGVNLTISNTAGAAKHQAVALRVSADNA 362

Query: 364 TFYSCIFEGYQDTLYAHSLRQFYRECDIYGTVDFIFGNAAVVLQNCNIYARLPLSGQFNA 423
           T Y+CIFEGYQDTLY HSLRQFYRECDIYGTVDFIFGNAAVVLQNCNIYARLPLSGQFNA
Sbjct: 363 TLYNCIFEGYQDTLYTHSLRQFYRECDIYGTVDFIFGNAAVVLQNCNIYARLPLSGQFNA 422

Query: 424 LTAQGRTDQNQNTGIFIHKCTIKPTPDLAASPTTKSYLGRPWKEFSRTVYMQSFIDAFID 483
           +TAQGRTD NQNTG  IH CTIK TP+LAASP TKSYLGRPWK++SRTVYMQSFID+FID
Sbjct: 423 VTAQGRTDPNQNTGTSIHNCTIKATPELAASPATKSYLGRPWKQYSRTVYMQSFIDSFID 482

Query: 484 PAGWKEWDGTLNLNTSYYAEFDNSGPGSDTSRRAQWAVGVINATVANIFTVSEILAGDQW 543
           PAGWKEWDGT+NLNTSYYAEF+NSGPGSDTS+R  WAVGVINATVAN FTVS+ LAGD+W
Sbjct: 483 PAGWKEWDGTMNLNTSYYAEFNNSGPGSDTSQRVSWAVGVINATVANSFTVSQFLAGDRW 542

Query: 544 LPPTAVPYSGGLIS 554
           LPPT VPY+GGLIS
Sbjct: 543 LPPTDVPYTGGLIS 555

BLAST of CmaCh20G011150 vs. NCBI nr
Match: gi|449461481|ref|XP_004148470.1| (PREDICTED: pectinesterase-like [Cucumis sativus])

HSP 1 Score: 871.3 bits (2250), Expect = 8.9e-250
Identity = 433/553 (78.30%), Postives = 480/553 (86.80%), Query Frame = 1

Query: 4   SSNPISLLTFFSLLFLFLFTSSSAVPTVEVCQSATSPSFCKSFLPSSPASVHSHCRYTLH 63
           SSNPISLLTF +LLFL L  SSSA PT ++CQS TSP FC SFLP SP S+HSHCR+TLH
Sbjct: 3   SSNPISLLTFLTLLFL-LSPSSSAAPTADICQSTTSPPFCNSFLPKSPDSIHSHCRFTLH 62

Query: 64  HALVQARTFITLVDLQLTLLPSFSALHDCRSLAMANLDFISSTVNTVNTTTNALPFYQAD 123
            AL  ARTF++LV+ QL LLPS SALHDCR LA ANLDF+  T + VN+TT  LP+Y A 
Sbjct: 63  QALAHARTFLSLVNAQLNLLPSLSALHDCRCLAEANLDFLFQTFSIVNSTTTTLPYYDAH 122

Query: 124 EMISLLSAVITNVDTCHDGLAG--SGLGLVDKVVKAISFDRQLYSLYLSLFKMGWVSKTM 183
           EM+SL+SA+ITNVDTC++GLA   S +GLVDKV++AISFD++LYSLYLSLFKMGWVSK +
Sbjct: 123 EMLSLISAIITNVDTCYEGLASLNSAVGLVDKVLEAISFDKKLYSLYLSLFKMGWVSKDL 182

Query: 184 KAPKLPKTNHFGFGNGPLKLKMSPKDRAYYERLMH-GKKPAARRLLQTNNADDGILVNGV 243
           KAP  PK NHFG G G LKLKMSPKDRAYYERL+H  K P ARRLLQTN  DDGI+VNG+
Sbjct: 183 KAPTFPKMNHFGAGKGQLKLKMSPKDRAYYERLVHRNKPPGARRLLQTNYQDDGIVVNGI 242

Query: 244 VAVDQNGAYDFTTINDAIAAAPNKSTAANGYFLIFVTAGIYMESVVVPKSKKYVLMIGEG 303
           V VDQNG YDFT I  AIAAAPNK+T A GYFLIFV AGIY E+V+VPK K+YVL+IGEG
Sbjct: 243 VGVDQNGMYDFTNITAAIAAAPNKTTVAKGYFLIFVAAGIYNETVLVPKEKRYVLLIGEG 302

Query: 304 NNRTIITGNKNVVDGSTTFNSATVIVEGTGFFGANLTITNTAGAIKHQAVALRVSADMTT 363
           NN+TIITGNKNVVDGSTTFNSATV VEGTGF G NLTITNTAG+ KHQAVALRVSAD  T
Sbjct: 303 NNQTIITGNKNVVDGSTTFNSATVAVEGTGFLGVNLTITNTAGSAKHQAVALRVSADNVT 362

Query: 364 FYSCIFEGYQDTLYAHSLRQFYRECDIYGTVDFIFGNAAVVLQNCNIYARLPLSGQFNAL 423
            Y+CIFEGYQDTLY HSLRQFYRECD+YGTVDFIFGNAAVVLQNCNIYARLP+SGQFNAL
Sbjct: 363 LYNCIFEGYQDTLYTHSLRQFYRECDVYGTVDFIFGNAAVVLQNCNIYARLPMSGQFNAL 422

Query: 424 TAQGRTDQNQNTGIFIHKCTIKPTPDLAASPTTKSYLGRPWKEFSRTVYMQSFIDAFIDP 483
           TAQGRTD NQNTG  IH CTIK TP+LAASP TKSYLGRPWK++SRTVYMQSFID+FIDP
Sbjct: 423 TAQGRTDPNQNTGTSIHNCTIKATPELAASPATKSYLGRPWKQYSRTVYMQSFIDSFIDP 482

Query: 484 AGWKEWDGTLNLNTSYYAEFDNSGPGSDTSRRAQWAVGVINATVANIFTVSEILAGDQWL 543
            GWKEWDGTLNLNTSYYAEF+NSGPG DTS+RA WAVGVINATVA+ FTVS++LAGD+WL
Sbjct: 483 VGWKEWDGTLNLNTSYYAEFNNSGPGCDTSQRASWAVGVINATVASNFTVSQLLAGDKWL 542

Query: 544 PPTAVPYSGGLIS 554
           PPT VPY+GGLIS
Sbjct: 543 PPTEVPYTGGLIS 554

BLAST of CmaCh20G011150 vs. NCBI nr
Match: gi|645258934|ref|XP_008235120.1| (PREDICTED: probable pectinesterase/pectinesterase inhibitor 7 [Prunus mume])

HSP 1 Score: 573.2 bits (1476), Expect = 5.0e-160
Identity = 307/553 (55.52%), Postives = 385/553 (69.62%), Query Frame = 1

Query: 10  LLTFFSLLFLFLFTSSSAVPTVEVCQSATSPSFCKSFLPSSPAS--VHSHCRYTLHHALV 69
           LL FF+ L L    SSS V    +C+S   PSFCKS LP +  +  V+ + R+++  +L 
Sbjct: 14  LLPFFASLSLAANDSSSPVSAGTICKSTPDPSFCKSVLPHNQTAQNVYYYGRFSVRKSLS 73

Query: 70  QARTFITLVDLQLTLLPSFS-----ALHDCRSLAMANLDFISSTVNTVNTTTNALPFYQA 129
           Q+R F+ LVD  L    S S     AL DCR LA  N+DF+ ++  TVN T+  L   +A
Sbjct: 74  QSRKFLNLVDKYLKRRSSLSLTAVRALEDCRLLAGLNMDFLLNSFETVNKTSKTLSNLKA 133

Query: 130 DEMISLLSAVITNVDTCHDGLAGSGLGLVDKVVKAISFDRQLYSLYLSLFKMGWVSKTMK 189
           D++ + LSA++TN  TC DG+  +      K   +   D + YS+ L+LF  GWV K  K
Sbjct: 134 DDVQTSLSAILTNQQTCLDGIQSTASAWSLKTGLSAPNDTKFYSVSLALFTKGWVPKKKK 193

Query: 190 -APKLPKTNHFGFGNGPLKLKMSPKDRAYYERLMHGKKPAARRLLQTNNADDGILVNGVV 249
            A   P  N  GF NG L LKMS + RA YE +        R++LQT   DDG+LV+ VV
Sbjct: 194 SATWKPTRNQRGFKNGRLPLKMSSRTRAVYEGV------TKRKMLQTETEDDGVLVSEVV 253

Query: 250 AVDQNGAYDFTTINDAIAAAPNKSTAANGYFLIFVTAGIYMESVVVPKSKKYVLMIGEGN 309
            V QNG  +F+TINDAIAAAPN S + +GYFLI+VTAG+Y E V + K+K+Y++M+G+G 
Sbjct: 254 TVSQNGTGNFSTINDAIAAAPNNSASTDGYFLIYVTAGVYEEYVSIAKNKRYLMMVGDGI 313

Query: 310 NRTIITGNKNVVDGSTTFNSATVIVEGTGFFGANLTITNTAGAIKHQAVALRVSADMTTF 369
           N+TI+TGN++VVDG TTFNSAT  V   GF   N+T  NTAGAIKHQAVA+R  AD++TF
Sbjct: 314 NQTILTGNRSVVDGWTTFNSATFAVTAPGFVAVNMTFRNTAGAIKHQAVAVRNGADLSTF 373

Query: 370 YSCIFEGYQDTLYAHSLRQFYRECDIYGTVDFIFGNAAVVLQNCNIYARLPLSGQFNALT 429
           YSC FE YQDTLY HSLRQFYRECDIYGTVDFIFGNAAVV QNCN+Y RLP+SGQFNA+T
Sbjct: 374 YSCSFEAYQDTLYTHSLRQFYRECDIYGTVDFIFGNAAVVFQNCNLYPRLPMSGQFNAIT 433

Query: 430 AQGRTDQNQNTGIFIHKCTIKPTPDLAAS-PTTKSYLGRPWKEFSRTVYMQSFIDAFIDP 489
           AQGRTD NQNTG  IH CTI+   DLA+S  ++K+YLGRPWKE+SRTVYMQS+ID+ +DP
Sbjct: 434 AQGRTDPNQNTGTSIHNCTIRAADDLASSNSSSKTYLGRPWKEYSRTVYMQSYIDSVVDP 493

Query: 490 AGWKEWDGTLNLNTSYYAEFDNSGPGSDTSRRAQW-AVGVINATVANIFTVSEILAGDQW 549
           AGW+ WDG   L+T YYAE++N+GPGS+T+ R  W    VINAT A  FTV+  L GD W
Sbjct: 494 AGWRAWDGEFALSTLYYAEYNNTGPGSNTTSRVTWTGYHVINATDAANFTVTNFLLGDDW 553

Query: 550 LPPTAVPYSGGLI 553
           LP T VPY+GGLI
Sbjct: 554 LPQTGVPYTGGLI 560

BLAST of CmaCh20G011150 vs. NCBI nr
Match: gi|595792043|ref|XP_007199770.1| (hypothetical protein PRUPE_ppa003639mg [Prunus persica])

HSP 1 Score: 572.8 bits (1475), Expect = 6.6e-160
Identity = 308/553 (55.70%), Postives = 382/553 (69.08%), Query Frame = 1

Query: 10  LLTFFSLLFLFLFTSSSAVPTVEVCQSATSPSFCKSFLPSSPAS--VHSHCRYTLHHALV 69
           L   FS L L    SSS V    +C+S   PSFCKS LP +  +  V+ + R+++  +L 
Sbjct: 14  LFPLFSSLSLAADNSSSPVSAGTICKSTPDPSFCKSVLPHNQTAENVYYYGRFSVRKSLS 73

Query: 70  QARTFITLVDLQLTLLPSFS-----ALHDCRSLAMANLDFISSTVNTVNTTTNALPFYQA 129
           Q+R F+ LVD  L    S S     AL DCR LA  N+DF+ ++  TVN T+  L   +A
Sbjct: 74  QSRKFLNLVDKYLKRRSSLSLTAVRALEDCRLLAGLNMDFLLNSFETVNKTSKTLSNLKA 133

Query: 130 DEMISLLSAVITNVDTCHDGLAGSGLGLVDKVVKAISFDRQLYSLYLSLFKMGWVSKTMK 189
           D++ + LSA++TN  TC DG+  +      K   +   D + YS+ L+LF  GWV K  K
Sbjct: 134 DDVQTSLSAILTNQQTCLDGIQSTASAWSLKTGLSAPNDTKFYSVSLALFTKGWVPKKKK 193

Query: 190 -APKLPKTNHFGFGNGPLKLKMSPKDRAYYERLMHGKKPAARRLLQTNNADDGILVNGVV 249
            A   P     GF NG L LKMS + RA YE +        R+LLQT   DDG+LV+ VV
Sbjct: 194 SATWKPTRKQRGFKNGRLPLKMSSRTRAVYEGV------TKRKLLQTETEDDGVLVSEVV 253

Query: 250 AVDQNGAYDFTTINDAIAAAPNKSTAANGYFLIFVTAGIYMESVVVPKSKKYVLMIGEGN 309
            V QNG  +F+TINDAIAAAPN S + NGYFLI+VTAG+Y E V + K+K+Y++M+G+G 
Sbjct: 254 TVSQNGTGNFSTINDAIAAAPNNSASTNGYFLIYVTAGVYEEYVSIAKNKRYLMMVGDGI 313

Query: 310 NRTIITGNKNVVDGSTTFNSATVIVEGTGFFGANLTITNTAGAIKHQAVALRVSADMTTF 369
           N+TI+TGN++VVDG TTFNSAT  V   GF   N+T  NTAGAIKHQAVA+R  AD++TF
Sbjct: 314 NQTILTGNRSVVDGWTTFNSATFAVTAPGFVAVNMTFRNTAGAIKHQAVAVRNGADLSTF 373

Query: 370 YSCIFEGYQDTLYAHSLRQFYRECDIYGTVDFIFGNAAVVLQNCNIYARLPLSGQFNALT 429
           YSC FE YQDTLY HSLRQFYRECDIYGTVDFIFGNAAVV QNCN+Y RLP+SGQFNA+T
Sbjct: 374 YSCSFEAYQDTLYTHSLRQFYRECDIYGTVDFIFGNAAVVFQNCNLYPRLPMSGQFNAIT 433

Query: 430 AQGRTDQNQNTGIFIHKCTIKPTPDLAAS-PTTKSYLGRPWKEFSRTVYMQSFIDAFIDP 489
           AQGRTD NQNTG  IH CTI+   DLA+S  +TK+YLGRPWKE+SRTVYMQS+ID+ +DP
Sbjct: 434 AQGRTDPNQNTGTSIHNCTIRAADDLASSNSSTKTYLGRPWKEYSRTVYMQSYIDSVVDP 493

Query: 490 AGWKEWDGTLNLNTSYYAEFDNSGPGSDTSRRAQW-AVGVINATVANIFTVSEILAGDQW 549
           AGW+ WDG   L+T YYAE++N+GPGS+T+ R  W    VINAT A  FTV+  L GD W
Sbjct: 494 AGWRAWDGEFALSTLYYAEYNNTGPGSNTTSRVTWPGYHVINATDAANFTVTNFLLGDDW 553

Query: 550 LPPTAVPYSGGLI 553
           LP T VPY+GGLI
Sbjct: 554 LPQTGVPYTGGLI 560

BLAST of CmaCh20G011150 vs. NCBI nr
Match: gi|356572750|ref|XP_003554529.1| (PREDICTED: probable pectinesterase/pectinesterase inhibitor 41 [Glycine max])

HSP 1 Score: 570.5 bits (1469), Expect = 3.3e-159
Identity = 307/555 (55.32%), Postives = 377/555 (67.93%), Query Frame = 1

Query: 13  FFSLLFLFLFTSSSAVPTVEV-------CQSATSPSFCKSFLPSSPASVHSHCRYTLHHA 72
           F  L+ L LF SS  VP           C+S   PSFCKS LP    +V+ + R+++  +
Sbjct: 8   FLFLILLPLFASSDTVPNPTTPVSPGTACKSTPDPSFCKSVLPPQNGNVYDYGRFSVKKS 67

Query: 73  LVQARTFITLVDLQLTLLPSFSA-----LHDCRSLAMANLDFISSTVNTVNTTTNALPFY 132
           L QAR F+ LVD  L    S SA     L DCR+L   N DF+SS+  TVN TT  LP +
Sbjct: 68  LSQARKFLNLVDKYLQRSSSLSATAIRALQDCRTLGELNFDFLSSSFQTVNKTTRFLPSF 127

Query: 133 QADEMISLLSAVITNVDTCHDGL--AGSGLGLVDKVVKAISFDRQLYSLYLSLFKMGWVS 192
           QAD++ +LLSA++TN  TC DGL    S   + + +   +S D +LYS+ L+LF  GWV 
Sbjct: 128 QADDIQTLLSAILTNQQTCLDGLKDTASAWSVRNGLTVPLSNDTKLYSVSLALFTKGWVP 187

Query: 193 KTMKAPKLPKTNHFGFGNGPLKLKMSPKDRAYYERLMHGKKPAARRLLQTNNADDGILVN 252
           +T      P     GF NG L LKMS + RA YE +      + R+LLQ    D+ ++V 
Sbjct: 188 RTKAKAMHPTKKQLGFKNGRLPLKMSSRTRAIYESV------SRRKLLQATVGDE-VVVR 247

Query: 253 GVVAVDQNGAYDFTTINDAIAAAPNKSTAANGYFLIFVTAGIYMESVVVPKSKKYVLMIG 312
            +V V Q+G+ +FTTINDAIAAAPNKS + +GYFLI+VTAG+Y E+V V K K Y++M+G
Sbjct: 248 DIVTVSQDGSGNFTTINDAIAAAPNKSVSTDGYFLIYVTAGVYEENVSVDKKKTYLMMVG 307

Query: 313 EGNNRTIITGNKNVVDGSTTFNSATVIVEGTGFFGANLTITNTAGAIKHQAVALRVSADM 372
           +G N+TIITGN++VVDG TTF+SAT+ V G GF G N+TI NTAGA+KHQAVALR  AD+
Sbjct: 308 DGINKTIITGNRSVVDGWTTFSSATLAVVGQGFVGVNMTIRNTAGAVKHQAVALRSGADL 367

Query: 373 TTFYSCIFEGYQDTLYAHSLRQFYRECDIYGTVDFIFGNAAVVLQNCNIYARLPLSGQFN 432
           +TFYSC FEGYQDTLY HSLRQFY ECDIYGTVDFIFGNA VV QNC +Y RLP+SGQFN
Sbjct: 368 STFYSCSFEGYQDTLYVHSLRQFYSECDIYGTVDFIFGNAKVVFQNCKMYPRLPMSGQFN 427

Query: 433 ALTAQGRTDQNQNTGIFIHKCTIKPTPDLAASPTTKSYLGRPWKEFSRTVYMQSFIDAFI 492
           A+TAQGRTD NQ+TGI IH CTI+   DLAAS    +YLGRPWKE+SRTVYMQ+ +D+ I
Sbjct: 428 AITAQGRTDPNQDTGISIHNCTIRAADDLAASNGVATYLGRPWKEYSRTVYMQTVMDSVI 487

Query: 493 DPAGWKEWDGTLNLNTSYYAEFDNSGPGSDTSRRAQW-AVGVINATVANIFTVSEILAGD 552
              GW+EWDG   L+T YYAE+ NSGPGS T  R  W    VINAT A  FTVS  L GD
Sbjct: 488 HAKGWREWDGDFALSTLYYAEYSNSGPGSGTDNRVTWPGYHVINATDAANFTVSNFLLGD 547