BLAST of Cucsa.202120 vs. Swiss-Prot
Match:
PME61_ARATH (Probable pectinesterase/pectinesterase inhibitor 61 OS=Arabidopsis thaliana GN=PME61 PE=2 SV=1)
HSP 1 Score: 709.5 bits (1830), Expect = 3.1e-203
Identity = 365/594 (61.45%), Postives = 438/594 (73.74%), Query Frame = 1
Query: 20 PAASFRLSGNDPPPPSSSLRRRSRILIISFMSVLLIAASVVSAAILVRSHMTSSQPHFPH 79
P+ + DP L+++++ +I F +L+ VV I S P
Sbjct: 8 PSGPSNPNQKDPATSLPELQKKTKTKLILFTLAVLVVG-VVCFGIFAGIRAVDSGKTEPK 67
Query: 80 --NKPSQAISRACSHTLYPSLCLSSLLSFPGAQSADVHDLVHISLNLTLQHLTKALYSTS 139
KP+QAISR CS +LYP+LC+ +LL FPG+ +AD ++L+HIS N TLQ +KALY++S
Sbjct: 68 LTRKPTQAISRTCSKSLYPNLCIDTLLDFPGSLTADENELIHISFNATLQKFSKALYTSS 127
Query: 140 QIPVLQISKDPLAHSAYEDCMELLNDAIDAFSLSL-----FSKDASNHDIMTWLSAALTY 199
I Q+ P SAY+ C+ELL+D++DA + +L S D S+ D+MTWLS+A+T
Sbjct: 128 TITYTQMP--PRVRSAYDSCLELLDDSVDALTRALSSVVVVSGDESHSDVMTWLSSAMTN 187
Query: 200 HDTCTAGFQDVADLG--VKDEVEAKLSDLSEMISNSLAIFSGFGG--GDLPVENRKRRRL 259
HDTCT GF ++ G VKD+V + DLSEM+SN LAIF+G +PV N R+L
Sbjct: 188 HDTCTDGFDEIEGQGGEVKDQVIGAVKDLSEMVSNCLAIFAGKVKDLSGVPVVNN--RKL 247
Query: 260 MESSTTSWAAENGGDHEGFPAWLSGKDRRLLAAPLSTIQADIVVAKDGSGKFKTVAEAIE 319
+ + T E P WL +DR LL P S IQADI V+KDGSG FKT+AEAI+
Sbjct: 248 LGTEET----------EELPNWLKREDRELLGTPTSAIQADITVSKDGSGTFKTIAEAIK 307
Query: 320 AAPSSSGRRIIIYIKAGKYEEENLKVGRKKTNLMFVGDGKGITVISGGKSVYDKVTTFRT 379
AP S RR +IY+KAG+YEEENLKVGRKKTNLMF+GDGKG TVI+GGKS+ D +TTF T
Sbjct: 308 KAPEHSSRRFVIYVKAGRYEEENLKVGRKKTNLMFIGDGKGKTVITGGKSIADDLTTFHT 367
Query: 380 ATFAGSGTNIILRDMTFENTAGPSKHQAVALRLSADHAVVYHCNIIGYQDTLYVHSNRQF 439
ATFA +G I+RDMTFEN AGP+KHQAVALR+ DHAVVY CNIIGYQD LYVHSNRQF
Sbjct: 368 ATFAATGAGFIVRDMTFENYAGPAKHQAVALRVGGDHAVVYRCNIIGYQDALYVHSNRQF 427
Query: 440 FRECDIYGTIDFIFGNAVVVFQSCNIYARKPMAGQKNTITAQDRKDPNQNTGISIHACKI 499
FREC+IYGT+DFIFGNA V+ QSCNIYARKPMA QK TITAQ+RKDPNQNTGISIHACK+
Sbjct: 428 FRECEIYGTVDFIFGNAAVILQSCNIYARKPMAQQKITITAQNRKDPNQNTGISIHACKL 487
Query: 500 VATGDLEASKGSFPTFLGRPWKLYSRVVYMVSSMGDHIHPRGWLEWQGSFALDTLYYGEY 559
+AT DLEASKGS+PT+LGRPWKLYSRVVYM+S MGDHI PRGWLEW G FALD+LYYGEY
Sbjct: 488 LATPDLEASKGSYPTYLGRPWKLYSRVVYMMSDMGDHIDPRGWLEWNGPFALDSLYYGEY 547
Query: 560 MNSGPGAAVGQRVKWPGYRVITSTVEASKFTVGQFIYGSSWLPSTGVSFSAGLS 603
MN G G+ +GQRVKWPGY VITSTVEASKFTV QFI GSSWLPSTGVSF +GLS
Sbjct: 548 MNKGLGSGIGQRVKWPGYHVITSTVEASKFTVAQFISGSSWLPSTGVSFFSGLS 586
BLAST of Cucsa.202120 vs. Swiss-Prot
Match:
PME34_ARATH (Probable pectinesterase/pectinesterase inhibitor 34 OS=Arabidopsis thaliana GN=PME34 PE=2 SV=1)
HSP 1 Score: 691.8 bits (1784), Expect = 6.6e-198
Identity = 347/599 (57.93%), Postives = 440/599 (73.46%), Query Frame = 1
Query: 13 SHSANTTPAASFRLSGNDPPPPSSSLRRRSRILIISF-MSVLLIAASVVSAAILVRSHMT 72
S + +TT A P +++ R + ++++ S +++ LI A+ + A + R +
Sbjct: 14 SVTTSTTTAPILNQVSTSEQPENNNRRSKKKLVVSSIVLAISLILAAAIFAGVRSRLKLN 73
Query: 73 SSQPHFPHNKPSQAISRACSHTLYPSLCLSSLLSFPGAQSADVH-DLVHISLNLTLQHLT 132
S P KPSQAIS+AC T +P LC+ SL+ FPG+ +A DL+H+++N+TL H +
Sbjct: 74 QSVPGLAR-KPSQAISKACELTRFPELCVDSLMDFPGSLAASSSKDLIHVTVNMTLHHFS 133
Query: 133 KALYSTSQIPVLQISKDPLAHSAYEDCMELLNDAIDAFSLSL---FSKDASNHDIMTWLS 192
ALYS++ + + + P A SAY+ C+ELL+D++DA S +L S A D+ TWLS
Sbjct: 134 HALYSSASLSFVDMP--PRARSAYDSCVELLDDSVDALSRALSSVVSSSAKPQDVTTWLS 193
Query: 193 AALTYHDTCTAGFQDVADLGVKDEVEAKLSDLSEMISNSLAIFSGFGGGD----LPVENR 252
AALT HDTCT GF V D GVKD + A L +LSE++SN LAIFS GD +P++NR
Sbjct: 194 AALTNHDTCTEGFDGVDDGGVKDHMTAALQNLSELVSNCLAIFSASHDGDDFAGVPIQNR 253
Query: 253 KRRRLMESSTTSWAAENGGDHEGFPAWLSGKDRRLLAAPLSTIQADIVVAKDGSGKFKTV 312
+ + E E FP W+ K+R +L P+S IQADI+V+KDG+G KT+
Sbjct: 254 RLLGVEERE------------EKFPRWMRPKEREILEMPVSQIQADIIVSKDGNGTCKTI 313
Query: 313 AEAIEAAPSSSGRRIIIYIKAGKYEEENLKVGRKKTNLMFVGDGKGITVISGGKSVYDKV 372
+EAI+ AP +S RRIIIY+KAG+YEE NLKVGRKK NLMFVGDGKG TVISGGKS++D +
Sbjct: 314 SEAIKKAPQNSTRRIIIYVKAGRYEENNLKVGRKKINLMFVGDGKGKTVISGGKSIFDNI 373
Query: 373 TTFRTATFAGSGTNIILRDMTFENTAGPSKHQAVALRLSADHAVVYHCNIIGYQDTLYVH 432
TTF TA+FA +G I RD+TFEN AGP+KHQAVALR+ ADHAV+Y CNIIGYQDTLYVH
Sbjct: 374 TTFHTASFAATGAGFIARDITFENWAGPAKHQAVALRIGADHAVIYRCNIIGYQDTLYVH 433
Query: 433 SNRQFFRECDIYGTIDFIFGNAVVVFQSCNIYARKPMAGQKNTITAQDRKDPNQNTGISI 492
SNRQFFRECDIYGT+DFIFGNA VV Q+C+IYARKPM QKNTITAQ+RKDPNQNTGISI
Sbjct: 434 SNRQFFRECDIYGTVDFIFGNAAVVLQNCSIYARKPMDFQKNTITAQNRKDPNQNTGISI 493
Query: 493 HACKIVATGDLEASKGSFPTFLGRPWKLYSRVVYMVSSMGDHIHPRGWLEWQGSFALDTL 552
HA +++A DL+A+ GS T+LGRPWKL+SR VYM+S +G H+H RGWLEW +FALDTL
Sbjct: 494 HASRVLAASDLQATNGSTQTYLGRPWKLFSRTVYMMSYIGGHVHTRGWLEWNTTFALDTL 553
Query: 553 YYGEYMNSGPGAAVGQRVKWPGYRVITSTVEASKFTVGQFIYGSSWLPSTGVSFSAGLS 603
YYGEY+NSGPG+ +GQRV WPGYRVI ST EA++FTV +FIYGSSWLPSTGVSF AGLS
Sbjct: 554 YYGEYLNSGPGSGLGQRVSWPGYRVINSTAEANRFTVAEFIYGSSWLPSTGVSFLAGLS 597
BLAST of Cucsa.202120 vs. Swiss-Prot
Match:
PME3_ARATH (Pectinesterase/pectinesterase inhibitor 3 OS=Arabidopsis thaliana GN=PME3 PE=2 SV=2)
HSP 1 Score: 474.6 bits (1220), Expect = 1.7e-132
Identity = 260/589 (44.14%), Postives = 364/589 (61.80%), Query Frame = 1
Query: 37 SLRRRSRILIISFMSVLLIAASV--VSAAILVRSHMTSSQPHFPHNKPSQAISRA-CSHT 96
+ ++ +++++S LL A+V +SA + + P S A+ R+ CS T
Sbjct: 13 NFKKNKKLVLLSAAVALLFVAAVAGISAGASKANEKRTLSPS------SHAVLRSSCSST 72
Query: 97 LYPSLCLSSLLSFPGAQSADVHDLVHISLNLTLQHLTKALYSTSQIPVLQISKDPLAHSA 156
YP LC+S++++ G + D++ S+NLT+ + ++ ++ + P +A
Sbjct: 73 RYPELCISAVVTAGGVELTSQKDVIEASVNLTITAVEHNYFTVKKLIKKRKGLTPREKTA 132
Query: 157 YEDCMELLNDAIDAF-----SLSLF--SKDASNH--DIMTWLSAALTYHDTCTAGFQ-DV 216
DC+E +++ +D L L+ K H D+ T +S+A+T +TC GF D
Sbjct: 133 LHDCLETIDETLDELHETVEDLHLYPTKKTLREHAGDLKTLISSAITNQETCLDGFSHDD 192
Query: 217 ADLGVKDEVEAKLSDLSEMISNSLAIFSGFGGGDLP-------VENRKRRRLMESSTTSW 276
AD V+ + + M SN+LA+ D+ + + R+ E+ T+
Sbjct: 193 ADKQVRKALLKGQIHVEHMCSNALAMIKNMTDTDIANFEQKAKITSNNRKLKEENQETTV 252
Query: 277 AAENGG----DHEGFPAWLSGKDRRLLAAPLSTIQADIVVAKDGSGKFKTVAEAIEAAPS 336
A + G D EG+P WLS DRRLL S ++AD VA DGSG FKTVA A+ AAP
Sbjct: 253 AVDIAGAGELDSEGWPTWLSAGDRRLLQG--SGVKADATVAADGSGTFKTVAAAVAAAPE 312
Query: 337 SSGRRIIIYIKAGKYEEENLKVGRKKTNLMFVGDGKGITVISGGKSVYDKVTTFRTATFA 396
+S +R +I+IKAG Y E N++V +KK N+MF+GDG+ T+I+G ++V D TTF +AT A
Sbjct: 313 NSNKRYVIHIKAGVYRE-NVEVAKKKKNIMFMGDGRTRTIITGSRNVVDGSTTFHSATVA 372
Query: 397 GSGTNIILRDMTFENTAGPSKHQAVALRLSADHAVVYHCNIIGYQDTLYVHSNRQFFREC 456
G + RD+TF+NTAGPSKHQAVALR+ +D + Y+C+++ YQDTLYVHSNRQFF +C
Sbjct: 373 AVGERFLARDITFQNTAGPSKHQAVALRVGSDFSAFYNCDMLAYQDTLYVHSNRQFFVKC 432
Query: 457 DIYGTIDFIFGNAVVVFQSCNIYARKPMAGQKNTITAQDRKDPNQNTGISIHACKIVATG 516
I GT+DFIFGNA VV Q C+I+AR+P +GQKN +TAQ R DPNQNTGI I C+I AT
Sbjct: 433 LIAGTVDFIFGNAAVVLQDCDIHARRPNSGQKNMVTAQGRTDPNQNTGIVIQKCRIGATS 492
Query: 517 DLEASKGSFPTFLGRPWKLYSRVVYMVSSMGDHIHPRGWLEWQGSFALDTLYYGEYMNSG 576
DL++ KGSFPT+LGRPWK YS+ V M S++ D I P GW EW G+FAL+TL Y EY N+G
Sbjct: 493 DLQSVKGSFPTYLGRPWKEYSQTVIMQSAISDVIRPEGWSEWTGTFALNTLTYREYSNTG 552
Query: 577 PGAAVGQRVKWPGYRVITSTVEASKFTVGQFIYGSSWLPSTGVSFSAGL 602
GA RVKW G++VIT+ EA K+T GQFI G WL STG FS GL
Sbjct: 553 AGAGTANRVKWRGFKVITAAAEAQKYTAGQFIGGGGWLSSTGFPFSLGL 592
BLAST of Cucsa.202120 vs. Swiss-Prot
Match:
PME2_ARATH (Pectinesterase 2 OS=Arabidopsis thaliana GN=PME2 PE=2 SV=2)
HSP 1 Score: 473.8 bits (1218), Expect = 2.8e-132
Identity = 263/580 (45.34%), Postives = 350/580 (60.34%), Query Frame = 1
Query: 36 SSLRRRSRILIISFMSVLLIAASVVSAAILVRSHMTSSQPHFPHNKPSQAISRA-CSHTL 95
S + ++++ S LL+ AS+V A ++ +Q + S AI ++ CS TL
Sbjct: 12 SDFKNNKKLILSSAAIALLLLASIVGIAATT-TNQNKNQKITTLSSTSHAILKSVCSSTL 71
Query: 96 YPSLCLSSLLSFPGAQSADVHDLVHISLNLTLQHLTKALYSTSQIPVLQISKDPLAHSAY 155
YP LC S++ + G + +++ SLNLT + + ++ ++ + P +A
Sbjct: 72 YPELCFSAVAATGGKELTSQKEVIEASLNLTTKAVKHNYFAVKKLIAKRKGLTPREVTAL 131
Query: 156 EDCMELLNDAIDAFSLSLFS-------KDASNH--DIMTWLSAALTYHDTCTAGFQ-DVA 215
DC+E +++ +D +++ K H D+ T +S+A+T TC GF D A
Sbjct: 132 HDCLETIDETLDELHVAVEDLHQYPKQKSLRKHADDLKTLISSAITNQGTCLDGFSYDDA 191
Query: 216 DLGVKDEVEAKLSDLSEMISNSLAIFSGFGGGDLP-VENRKRRRLMESSTTSWAAENGGD 275
D V+ + + M SN+LA+ D+ E R + ++ E GD
Sbjct: 192 DRKVRKALLKGQVHVEHMCSNALAMIKNMTETDIANFELRDKSSTFTNNNNRKLKEVTGD 251
Query: 276 --HEGFPAWLSGKDRRLLAAPLSTIQADIVVAKDGSGKFKTVAEAIEAAPSSSGRRIIIY 335
+G+P WLS DRRLL STI+AD VA DGSG F TVA A+ AAP S +R +I+
Sbjct: 252 LDSDGWPKWLSVGDRRLLQG--STIKADATVADDGSGDFTTVAAAVAAAPEKSNKRFVIH 311
Query: 336 IKAGKYEEENLKVGRKKTNLMFVGDGKGITVISGGKSVYDKVTTFRTATFAGSGTNIILR 395
IKAG Y E N++V +KKTN+MF+GDG+G T+I+G ++V D TTF +AT A G + R
Sbjct: 312 IKAGVYRE-NVEVTKKKTNIMFLGDGRGKTIITGSRNVVDGSTTFHSATVAAVGERFLAR 371
Query: 396 DMTFENTAGPSKHQAVALRLSADHAVVYHCNIIGYQDTLYVHSNRQFFRECDIYGTIDFI 455
D+TF+NTAGPSKHQAVALR+ +D + Y C++ YQDTLYVHSNRQFF +C I GT+DFI
Sbjct: 372 DITFQNTAGPSKHQAVALRVGSDFSAFYQCDMFAYQDTLYVHSNRQFFVKCHITGTVDFI 431
Query: 456 FGNAVVVFQSCNIYARKPMAGQKNTITAQDRKDPNQNTGISIHACKIVATGDLEASKGSF 515
FGNA V Q C+I AR+P +GQKN +TAQ R DPNQNTGI I C+I T DL A KG+F
Sbjct: 432 FGNAAAVLQDCDINARRPNSGQKNMVTAQGRSDPNQNTGIVIQNCRIGGTSDLLAVKGTF 491
Query: 516 PTFLGRPWKLYSRVVYMVSSMGDHIHPRGWLEWQGSFALDTLYYGEYMNSGPGAAVGQRV 575
PT+LGRPWK YSR V M S + D I P GW EW GSFALDTL Y EY+N G GA RV
Sbjct: 492 PTYLGRPWKEYSRTVIMQSDISDVIRPEGWHEWSGSFALDTLTYREYLNRGGGAGTANRV 551
Query: 576 KWPGYRVITSTVEASKFTVGQFIYGSSWLPSTGVSFSAGL 602
KW GY+VITS EA FT GQFI G WL STG FS L
Sbjct: 552 KWKGYKVITSDTEAQPFTAGQFIGGGGWLASTGFPFSLSL 587
BLAST of Cucsa.202120 vs. Swiss-Prot
Match:
PME3_CITSI (Pectinesterase 3 OS=Citrus sinensis PE=1 SV=1)
HSP 1 Score: 471.5 bits (1212), Expect = 1.4e-131
Identity = 264/591 (44.67%), Postives = 365/591 (61.76%), Query Frame = 1
Query: 25 RLSGNDPPPPSSSLRRRSRILIISFMSVLLIAASVVSAAILVRSHMTSSQPHFPHNKPSQ 84
+LS + S++ ++ + L ++ + LL+ A+V+ V S S N+P
Sbjct: 10 KLSESSTNQNISNIPKKKKKLFLALFATLLVVAAVIGIVAGVNSRKNSGDNG---NEPHH 69
Query: 85 AISRA-CSHTLYPSLCLSSLLSFPGAQS--ADVHDLVHISLNLTLQHLTKALYSTSQIPV 144
AI ++ CS T YP LC S++ + P A D++ +SLN+T + + ++ +
Sbjct: 70 AILKSSCSSTRYPDLCFSAIAAVPEASKKVTSQKDVIEMSLNITTTAVEHNYFGIQKL-L 129
Query: 145 LQISKDPLAHSAYEDCMELLNDAIDAFSLSLFS-------KDASNH--DIMTWLSAALTY 204
+ + A DC+E +++ +D ++ K S H D+ T +SAA+T
Sbjct: 130 KRTNLTKREKVALHDCLETIDETLDELHKAVEDLEEYPNKKSLSQHADDLKTLMSAAMTN 189
Query: 205 HDTCTAGFQ-DVADLGVKDEVEAKLSDLSEMISNSLAIFSGFGGGDLPV-ENRKRRRLME 264
TC GF D A+ V+D + + +M SN+LA+ D+ + R+L+E
Sbjct: 190 QGTCLDGFSHDDANKHVRDALSDGQVHVEKMCSNALAMIKNMTDTDMMIMRTSNNRKLIE 249
Query: 265 SSTTSWAAENGGDHEGFPAWLSGKDRRLLAAPLSTIQADIVVAKDGSGKFKTVAEAIEAA 324
++T +G+PAWLS DRRLL + S++ ++VVA DGSG FKTVA ++ AA
Sbjct: 250 ETSTV---------DGWPAWLSTGDRRLLQS--SSVTPNVVVAADGSGNFKTVAASVAAA 309
Query: 325 PSSSGRRIIIYIKAGKYEEENLKVGRKKTNLMFVGDGKGITVISGGKSVYDKVTTFRTAT 384
P +R II IKAG Y E N++V +K N+MF+GDG+ T+I+G ++V D TTF++AT
Sbjct: 310 PQGGTKRYIIRIKAGVYRE-NVEVTKKHKNIMFIGDGRTRTIITGSRNVVDGSTTFKSAT 369
Query: 385 FAGSGTNIILRDMTFENTAGPSKHQAVALRLSADHAVVYHCNIIGYQDTLYVHSNRQFFR 444
A G + RD+TF+NTAGPSKHQAVALR+ AD + Y+C+++ YQDTLYVHSNRQFF
Sbjct: 370 VAVVGEGFLARDITFQNTAGPSKHQAVALRVGADLSAFYNCDMLAYQDTLYVHSNRQFFV 429
Query: 445 ECDIYGTIDFIFGNAVVVFQSCNIYARKPMAGQKNTITAQDRKDPNQNTGISIHACKIVA 504
C I GT+DFIFGNA V Q+C+I+ARKP +GQKN +TAQ R DPNQNTGI I +I A
Sbjct: 430 NCLIAGTVDFIFGNAAAVLQNCDIHARKPNSGQKNMVTAQGRADPNQNTGIVIQKSRIGA 489
Query: 505 TGDLEASKGSFPTFLGRPWKLYSRVVYMVSSMGDHIHPRGWLEWQGSFALDTLYYGEYMN 564
T DL+ +GSFPT+LGRPWK YSR V M SS+ D IHP GW EW G+FAL+TL+YGE+ N
Sbjct: 490 TSDLKPVQGSFPTYLGRPWKEYSRTVIMQSSITDVIHPAGWHEWDGNFALNTLFYGEHQN 549
Query: 565 SGPGAAVGQRVKWPGYRVITSTVEASKFTVGQFIYGSSWLPSTGVSFSAGL 602
+G GA RVKW G+RVITS EA FT G FI GSSWL STG FS GL
Sbjct: 550 AGAGAGTSGRVKWKGFRVITSATEAQAFTPGSFIAGSSWLGSTGFPFSLGL 584
BLAST of Cucsa.202120 vs. TrEMBL
Match:
A0A0A0KJG1_CUCSA (Pectinesterase OS=Cucumis sativus GN=Csa_5G139430 PE=4 SV=1)
HSP 1 Score: 1210.7 bits (3131), Expect = 0.0e+00
Identity = 604/604 (100.00%), Postives = 604/604 (100.00%), Query Frame = 1
Query: 1 MNNNNSYNKLHTSHSANTTPAASFRLSGNDPPPPSSSLRRRSRILIISFMSVLLIAASVV 60
MNNNNSYNKLHTSHSANTTPAASFRLSGNDPPPPSSSLRRRSRILIISFMSVLLIAASVV
Sbjct: 1 MNNNNSYNKLHTSHSANTTPAASFRLSGNDPPPPSSSLRRRSRILIISFMSVLLIAASVV 60
Query: 61 SAAILVRSHMTSSQPHFPHNKPSQAISRACSHTLYPSLCLSSLLSFPGAQSADVHDLVHI 120
SAAILVRSHMTSSQPHFPHNKPSQAISRACSHTLYPSLCLSSLLSFPGAQSADVHDLVHI
Sbjct: 61 SAAILVRSHMTSSQPHFPHNKPSQAISRACSHTLYPSLCLSSLLSFPGAQSADVHDLVHI 120
Query: 121 SLNLTLQHLTKALYSTSQIPVLQISKDPLAHSAYEDCMELLNDAIDAFSLSLFSKDASNH 180
SLNLTLQHLTKALYSTSQIPVLQISKDPLAHSAYEDCMELLNDAIDAFSLSLFSKDASNH
Sbjct: 121 SLNLTLQHLTKALYSTSQIPVLQISKDPLAHSAYEDCMELLNDAIDAFSLSLFSKDASNH 180
Query: 181 DIMTWLSAALTYHDTCTAGFQDVADLGVKDEVEAKLSDLSEMISNSLAIFSGFGGGDLPV 240
DIMTWLSAALTYHDTCTAGFQDVADLGVKDEVEAKLSDLSEMISNSLAIFSGFGGGDLPV
Sbjct: 181 DIMTWLSAALTYHDTCTAGFQDVADLGVKDEVEAKLSDLSEMISNSLAIFSGFGGGDLPV 240
Query: 241 ENRKRRRLMESSTTSWAAENGGDHEGFPAWLSGKDRRLLAAPLSTIQADIVVAKDGSGKF 300
ENRKRRRLMESSTTSWAAENGGDHEGFPAWLSGKDRRLLAAPLSTIQADIVVAKDGSGKF
Sbjct: 241 ENRKRRRLMESSTTSWAAENGGDHEGFPAWLSGKDRRLLAAPLSTIQADIVVAKDGSGKF 300
Query: 301 KTVAEAIEAAPSSSGRRIIIYIKAGKYEEENLKVGRKKTNLMFVGDGKGITVISGGKSVY 360
KTVAEAIEAAPSSSGRRIIIYIKAGKYEEENLKVGRKKTNLMFVGDGKGITVISGGKSVY
Sbjct: 301 KTVAEAIEAAPSSSGRRIIIYIKAGKYEEENLKVGRKKTNLMFVGDGKGITVISGGKSVY 360
Query: 361 DKVTTFRTATFAGSGTNIILRDMTFENTAGPSKHQAVALRLSADHAVVYHCNIIGYQDTL 420
DKVTTFRTATFAGSGTNIILRDMTFENTAGPSKHQAVALRLSADHAVVYHCNIIGYQDTL
Sbjct: 361 DKVTTFRTATFAGSGTNIILRDMTFENTAGPSKHQAVALRLSADHAVVYHCNIIGYQDTL 420
Query: 421 YVHSNRQFFRECDIYGTIDFIFGNAVVVFQSCNIYARKPMAGQKNTITAQDRKDPNQNTG 480
YVHSNRQFFRECDIYGTIDFIFGNAVVVFQSCNIYARKPMAGQKNTITAQDRKDPNQNTG
Sbjct: 421 YVHSNRQFFRECDIYGTIDFIFGNAVVVFQSCNIYARKPMAGQKNTITAQDRKDPNQNTG 480
Query: 481 ISIHACKIVATGDLEASKGSFPTFLGRPWKLYSRVVYMVSSMGDHIHPRGWLEWQGSFAL 540
ISIHACKIVATGDLEASKGSFPTFLGRPWKLYSRVVYMVSSMGDHIHPRGWLEWQGSFAL
Sbjct: 481 ISIHACKIVATGDLEASKGSFPTFLGRPWKLYSRVVYMVSSMGDHIHPRGWLEWQGSFAL 540
Query: 541 DTLYYGEYMNSGPGAAVGQRVKWPGYRVITSTVEASKFTVGQFIYGSSWLPSTGVSFSAG 600
DTLYYGEYMNSGPGAAVGQRVKWPGYRVITSTVEASKFTVGQFIYGSSWLPSTGVSFSAG
Sbjct: 541 DTLYYGEYMNSGPGAAVGQRVKWPGYRVITSTVEASKFTVGQFIYGSSWLPSTGVSFSAG 600
Query: 601 LSTN 605
LSTN
Sbjct: 601 LSTN 604
BLAST of Cucsa.202120 vs. TrEMBL
Match:
A0ZNJ9_PYRCO (Pectinesterase OS=Pyrus communis GN=PcPME1 PE=2 SV=1)
HSP 1 Score: 749.6 bits (1934), Expect = 3.0e-213
Identity = 375/584 (64.21%), Postives = 448/584 (76.71%), Query Frame = 1
Query: 35 SSSLRRRSRILIISFMSVLLIAASVVSAAILVRSHMTSSQPHFP---HNKPSQAISRACS 94
S+S R++++++IS +SV LI AS VSA +L +S H KP++AIS AC+
Sbjct: 35 STSRSRKNKLILISLLSVALILASAVSAVVLFAGRSKASNSAASSALHRKPTKAISDACA 94
Query: 95 HTLYPSLCLSSLLSFPGAQSADVHDLVHISLNLTLQHLTKALYSTSQIPVLQISKDPLAH 154
T +PSLC+ SLL FPG+ +A DLVHIS N+TLQ L+KALY +S + Q+ DP +
Sbjct: 95 TTRFPSLCVDSLLDFPGSTTASEQDLVHISFNMTLQRLSKALYLSSSLSYRQM--DPYSR 154
Query: 155 SAYEDCMELLNDAIDAFSLSLFS---------KDASNHDIMTWLSAALTYHDTCTAGFQD 214
SAY+DC+ELL+D++DA S +L S S D++TWLSAALT DTC GF+
Sbjct: 155 SAYDDCLELLDDSVDALSRALTSVIPGAASTSTSTSTQDVLTWLSAALTNQDTCGEGFEQ 214
Query: 215 VADLGVKDEVEAKLSDLSEMISNSLAIFSGFGGGD---LPVENRKRRRLMESSTT-SWAA 274
+ VK+E++ +L DLSE++SN LAI+S GGGD +P++NR+RR L S
Sbjct: 215 INGGDVKNEMDQRLKDLSELVSNCLAIYSAIGGGDFSGVPIQNRRRRLLGNSDMERDIMG 274
Query: 275 ENGGDHEGFPAWLSGKDRRLLAAPLSTIQADIVVAKDGSGKFKTVAEAIEAAPSSSGRRI 334
+N FP WL ++RRLLA P S IQADI+V+KDGSG FKT+AEAI+ AP SS RR
Sbjct: 275 DNANVSRDFPRWLGRRERRLLAVPGSQIQADIIVSKDGSGTFKTIAEAIKKAPESSSRRT 334
Query: 335 IIYIKAGKYEEENLKVGRKKTNLMFVGDGKGITVISGGKSVYDKVTTFRTATFAGSGTNI 394
IIY+KAG+YEE NLKV RKKTNLMF+GDGKG T+I+GGK+V KVTTF TATFA G
Sbjct: 335 IIYVKAGRYEESNLKVARKKTNLMFIGDGKGKTIITGGKNVAQKVTTFHTATFAAQGAGF 394
Query: 395 ILRDMTFENTAGPSKHQAVALRLSADHAVVYHCNIIGYQDTLYVHSNRQFFRECDIYGTI 454
I RD+TFEN AGP KHQAVALR+ ADHAVVYHC+IIGYQDT YVHSNRQF RE DIYGT+
Sbjct: 395 IARDLTFENYAGPDKHQAVALRIGADHAVVYHCSIIGYQDTFYVHSNRQFVRETDIYGTV 454
Query: 455 DFIFGNAVVVFQSCNIYARKPMAGQKNTITAQDRKDPNQNTGISIHACKIVATGDLEASK 514
DFIFGNA VVFQ C++YARKPMA QKNTITAQ+RKDPNQNTGISIH C+I+AT +LE SK
Sbjct: 455 DFIFGNAAVVFQKCSLYARKPMANQKNTITAQNRKDPNQNTGISIHDCRILATPELEGSK 514
Query: 515 GSFPTFLGRPWKLYSRVVYMVSSMGDHIHPRGWLEWQGSFALDTLYYGEYMNSGPGAAVG 574
GSFPT+LGRPWKLYSR VYM+S +GDH+HPRGWLEW G+FALDTLYYGEYMNSGPGAAVG
Sbjct: 515 GSFPTYLGRPWKLYSRTVYMLSFIGDHVHPRGWLEWNGNFALDTLYYGEYMNSGPGAAVG 574
Query: 575 QRVKWPGYRVITSTVEASKFTVGQFIYGSSWLPSTGVSFSAGLS 603
QRV WPGYRVITS VEA KFTV QFIYGSSWLPSTGV+F AGLS
Sbjct: 575 QRVTWPGYRVITSPVEAGKFTVAQFIYGSSWLPSTGVAFLAGLS 616
BLAST of Cucsa.202120 vs. TrEMBL
Match:
V4TRH7_9ROSI (Pectinesterase OS=Citrus clementina GN=CICLE_v10019376mg PE=4 SV=1)
HSP 1 Score: 745.0 bits (1922), Expect = 7.4e-212
Identity = 374/586 (63.82%), Postives = 450/586 (76.79%), Query Frame = 1
Query: 28 GNDPPPPSSSLRRRSRILIISFMSVLLIAASVVSAAILVRSHMTSSQPHFPHNKPSQAIS 87
G+ P S R R +++++S +S LI AS VSA +LV ++ P H KP+QAIS
Sbjct: 23 GHATSAPQPSSRSRKKLILLSVLSFALIIASAVSAVLLVGLKTSAVGPTIGH-KPTQAIS 82
Query: 88 RACSHTLYPSLCLSSLLSFPGAQSADVHDLVHISLNLTLQHLTKALYSTSQIPVLQISKD 147
R CS TLYP+LC+SSLL+FPG+ +A DLVHIS N+TLQH ++ALY TS I LQ+ D
Sbjct: 83 RTCSKTLYPNLCVSSLLNFPGSLTASEQDLVHISFNMTLQHFSRALYFTSGISYLQM--D 142
Query: 148 PLAHSAYEDCMELLNDAIDAFSLSLFS-------KDASNHDIMTWLSAALTYHDTCTAGF 207
P SA++DC+ELL+D++DA S SL S D++TWLSAALT HDTC+ GF
Sbjct: 143 PHVRSAFDDCLELLDDSVDALSRSLGSVLLLPGGDINKREDVLTWLSAALTNHDTCSEGF 202
Query: 208 QDVADLGVKDEVEAKLSDLSEMISNSLAIFSGFGGGD----LPVENRKRRRLMESSTTSW 267
+++ VKD+V KL DLSE++SN LAIFS G D +P++NR+RR L S
Sbjct: 203 EELNG-PVKDQVSDKLKDLSELVSNCLAIFSAGGDSDDFSGVPIQNRRRRLLSSSEEDDT 262
Query: 268 AAENGGDHEGFPAWLSGKDRRLLAAPLSTIQADIVVAKDGSGKFKTVAEAIEAAPSSSGR 327
EN D + P W+S + RRLL+ P+ IQADI+V+KDG+G KT+AEAI+ AP +S R
Sbjct: 263 WGENDDD-DNVPRWVSRETRRLLSLPVPHIQADIIVSKDGTGTVKTIAEAIKKAPENSDR 322
Query: 328 RIIIYIKAGKYEEENLKVGRKKTNLMFVGDGKGITVISGGKSVYDKVTTFRTATFAGSGT 387
R IIY++AG+YEE NLKVGRKK NLMF+GDGKG T+I+GG++V+DK+TTF TA+FA +G
Sbjct: 323 RTIIYVRAGRYEESNLKVGRKKRNLMFIGDGKGKTIITGGRNVFDKLTTFHTASFAATGA 382
Query: 388 NIILRDMTFENTAGPSKHQAVALRLSADHAVVYHCNIIGYQDTLYVHSNRQFFRECDIYG 447
I RDMTFEN AGP+KHQAVALR+ ADHAVVY CN+IGYQDTLYVHSNRQF+RECDIYG
Sbjct: 383 GFIARDMTFENFAGPAKHQAVALRVGADHAVVYRCNVIGYQDTLYVHSNRQFYRECDIYG 442
Query: 448 TIDFIFGNAVVVFQSCNIYARKPMAGQKNTITAQDRKDPNQNTGISIHACKIVATGDLEA 507
T+DFIFGNA VV Q C IYARKPMA QKNTITAQ+RKDPNQNTGISIH C + A DL+A
Sbjct: 443 TVDFIFGNAAVVLQKCAIYARKPMANQKNTITAQNRKDPNQNTGISIHDCSLYAAPDLQA 502
Query: 508 SKGSFPTFLGRPWKLYSRVVYMVSSMGDHIHPRGWLEWQGSFALDTLYYGEYMNSGPGAA 567
SKGS PT+LGRPWKLYSRVVYM++ MGDHI PRGWLEW G FALDTLYYGEYMNSGPG+A
Sbjct: 503 SKGSIPTYLGRPWKLYSRVVYMLTYMGDHIQPRGWLEWNGDFALDTLYYGEYMNSGPGSA 562
Query: 568 VGQRVKWPGYRVITSTVEASKFTVGQFIYGSSWLPSTGVSFSAGLS 603
VG RV+WPGYRVITS EASKFTV QFIYGSSWLPSTGV+F GLS
Sbjct: 563 VGHRVRWPGYRVITSATEASKFTVSQFIYGSSWLPSTGVAFLGGLS 603
BLAST of Cucsa.202120 vs. TrEMBL
Match:
W9RDL0_9ROSA (Pectinesterase OS=Morus notabilis GN=L484_006819 PE=4 SV=1)
HSP 1 Score: 740.0 bits (1909), Expect = 2.4e-210
Identity = 392/621 (63.12%), Postives = 464/621 (74.72%), Query Frame = 1
Query: 7 YNKLHTSHSANTTPAASFRLSGNDPPPPSSSLRRRSRILII-SFMSVLLIAASVVSAAIL 66
Y KL S ++ S LS SS R + ++L++ S +S++LI AS VSA L
Sbjct: 3 YGKLEPSDPGGSS---SMDLSHQSEHATSSGNRSKKKLLVVLSVVSIVLITASAVSAVFL 62
Query: 67 V----RSHMTSSQPHFPHNKPSQAISRACSHTLYPSLCLSSLLSFPGAQSADVHDLVHIS 126
+ R+ S P H KP+QAISRACS T +PSLC++SL+ FPGA +A DLVHIS
Sbjct: 63 IGVRTRASGQSGHPGLAH-KPTQAISRACSKTRFPSLCVNSLVDFPGALTATEEDLVHIS 122
Query: 127 LNLTLQHLTKALYSTSQIPVLQISKDPLAHSAYEDCMELLNDAIDAFSLSLFS------- 186
N+TLQ L+KALY++S I L + DPL+ SAY+ C+ELL+D+IDA S SL S
Sbjct: 123 FNMTLQRLSKALYASSGISYLAM--DPLSRSAYDGCLELLDDSIDALSRSLTSVAPSVAG 182
Query: 187 ------KDASNHDIMTWLSAALTYHDTCTAGFQDVADLGVKDEVEAKLSDLSEMISNSLA 246
AS+ D++TWLSAALT DTC GF VA VK + L DLSE++SN L+
Sbjct: 183 GSAKPISGASSQDVVTWLSAALTNQDTCGEGFDGVAGGSVKSHMAESLRDLSELLSNCLS 242
Query: 247 IFSGFGGGD---LPVENRKRRRLMESSTTSWAAENGGDHEGFPAWLSGKDRRLLAAPLST 306
IFSG D +P++NR RRLM+ S GD FP WL K+RRLLA P+S
Sbjct: 243 IFSGSVTDDFSGVPIQNR--RRLMQEDDIS------GD---FPRWLGRKERRLLALPVSA 302
Query: 307 IQADIVVAKDGSG----KFKTVAEAIEAAPSSSGRRIIIYIKAGKYEEENLKVGRKKTNL 366
IQADIVV+KDG+G ++KT+AEAI+ P S RRI+IY+KAG+YEE NLKVGRKKTN+
Sbjct: 303 IQADIVVSKDGNGSNGREYKTIAEAIKKVPEHSTRRIVIYVKAGRYEENNLKVGRKKTNV 362
Query: 367 MFVGDGKGITVISGGKSVYDKVTTFRTATFAGSGTNIILRDMTFENTAGPSKHQAVALRL 426
MF+GDG G TVISGGKSV DK+TTF TA+FA +G I RD+TF N AGP +HQAVALR+
Sbjct: 363 MFIGDGAGKTVISGGKSVSDKLTTFHTASFAATGAGFIARDITFTNWAGPGRHQAVALRI 422
Query: 427 SADHAVVYHCNIIGYQDTLYVHSNRQFFRECDIYGTIDFIFGNAVVVFQSCNIYARKPMA 486
ADHAVVY C IGYQDTLYVHSNRQFFREC+IYGT+DFIFGNA VVFQ+C+IYARKPM
Sbjct: 423 GADHAVVYRCQAIGYQDTLYVHSNRQFFRECEIYGTVDFIFGNAAVVFQNCSIYARKPMP 482
Query: 487 GQKNTITAQDRKDPNQNTGISIHACKIVATGDLEASKGSFPTFLGRPWKLYSRVVYMVSS 546
QKNTITAQ+RKDPNQNTGISIHAC+I+AT DL+ASKGSFPT+LGRPWKLYSRVVY++S
Sbjct: 483 QQKNTITAQNRKDPNQNTGISIHACRILATSDLQASKGSFPTYLGRPWKLYSRVVYLMSY 542
Query: 547 MGDHIHPRGWLEWQGSFALDTLYYGEYMNSGPGAAVGQRVKWPGYRVITSTVEASKFTVG 603
MGDHIHPRGWLEW +FALD+LYYGEYMNSGPG AVGQRVKWPGYRVITSTVEASKFTV
Sbjct: 543 MGDHIHPRGWLEWNATFALDSLYYGEYMNSGPGGAVGQRVKWPGYRVITSTVEASKFTVA 602
BLAST of Cucsa.202120 vs. TrEMBL
Match:
A0A067JU95_JATCU (Pectinesterase OS=Jatropha curcas GN=JCGZ_20123 PE=4 SV=1)
HSP 1 Score: 739.6 bits (1908), Expect = 3.1e-210
Identity = 378/611 (61.87%), Postives = 453/611 (74.14%), Query Frame = 1
Query: 11 HTSHSANTTPAASFRLSGNDPPPPSSSLRRRSRILIISFMSVLLIAASVVSAAILVRSHM 70
H P S R S RR+ +I+++S +S+ LI AS VSA +L+
Sbjct: 5 HYGRLGPAEPGGSSRHLDQPEYATSQPSRRKKKIILLSLLSLALIIASAVSATLLIGLRT 64
Query: 71 TSSQPHFP--HNKPSQAISRACSHTLYPSLCLSSLLSFPGAQSADVHDLVHISLNLTLQH 130
+S P KP++AIS+AC T +P+LC++SLL FPG+ +A DLVHIS N+TLQH
Sbjct: 65 RASGQPGPALDRKPTKAISKACRKTRFPTLCVNSLLDFPGSTTASEKDLVHISFNMTLQH 124
Query: 131 LTKALYSTSQIPVLQISKDPLAHSAYEDCMELLNDAIDAFSLSLF--------------S 190
+KALY +S I LQ+ D SAYEDC+ELL+D++DA S SL S
Sbjct: 125 FSKALYLSSAISYLQM--DTRVRSAYEDCLELLDDSVDALSRSLSTVAPPIDGNTGQIQS 184
Query: 191 KDASNHDIMTWLSAALTYHDTCTAGFQDVADLGVKDEVEAKLSDLSEMISNSLAIFSGFG 250
S D++ WLSAALT DTCT GF D+ VK+++ KL DLSE++SN LAIFS
Sbjct: 185 AGGSTEDVVNWLSAALTNQDTCTEGFADLNG-AVKEQMTVKLKDLSELVSNCLAIFSASD 244
Query: 251 GGD---LPVENRKRRRLMESSTTSWAAENGGDHEGFPAWLSGKDRRLLAAPLSTIQADIV 310
D +P++NR+R + E S G + + P+WL ++RRLL P+S IQADI+
Sbjct: 245 DDDFSGVPIQNRRRLMVAEIS--------GNNADELPSWLGRRERRLLDMPVSAIQADII 304
Query: 311 VAKDGSGKFKTVAEAIEAAPSSSGRRIIIYIKAGKYEEENLKVGRKKTNLMFVGDGKGIT 370
V++ G+G +KT+ EAI+ AP S RR IIY+ AG+YEE+ LKVGRKK NLMF+GDGKG T
Sbjct: 305 VSQYGNGTYKTITEAIKKAPEYSNRRTIIYVTAGRYEEKILKVGRKKRNLMFIGDGKGKT 364
Query: 371 VISGGKSVYDKVTTFRTATFAGSGTNIILRDMTFENTAGPSKHQAVALRLSADHAVVYHC 430
+ISGGKS++D +TTF TATFA +G I RDMTFEN AGPSKHQAVALR+ ADHAVVY C
Sbjct: 365 IISGGKSIFDNITTFHTATFAATGAGFIARDMTFENWAGPSKHQAVALRIGADHAVVYRC 424
Query: 431 NIIGYQDTLYVHSNRQFFRECDIYGTIDFIFGNAVVVFQSCNIYARKPMAGQKNTITAQD 490
NIIGYQDTLYVHSNRQFFRECDIYGT+DFIFGNA VVFQ+C+IYARKPMA QKNTITAQ+
Sbjct: 425 NIIGYQDTLYVHSNRQFFRECDIYGTVDFIFGNAAVVFQNCSIYARKPMAFQKNTITAQN 484
Query: 491 RKDPNQNTGISIHACKIVATGDLEASKGSFPTFLGRPWKLYSRVVYMVSSMGDHIHPRGW 550
RKDPNQNTGISIHAC+I+AT DLEASKGSFPT+LGRPWKLYSRVVYM+S MGDHIHPRGW
Sbjct: 485 RKDPNQNTGISIHACRILATSDLEASKGSFPTYLGRPWKLYSRVVYMLSYMGDHIHPRGW 544
Query: 551 LEWQGSFALDTLYYGEYMNSGPGAAVGQRVKWPGYRVITSTVEASKFTVGQFIYGSSWLP 603
LEW +FALDTLYYGEYMN GPG A+GQRVKWPGYRVITSTVEASKFTV +FIYGSSWLP
Sbjct: 545 LEWNTTFALDTLYYGEYMNDGPGGALGQRVKWPGYRVITSTVEASKFTVAEFIYGSSWLP 604
BLAST of Cucsa.202120 vs. TAIR10
Match:
AT5G53370.1 (AT5G53370.1 pectin methylesterase PCR fragment F)
HSP 1 Score: 709.5 bits (1830), Expect = 1.7e-204
Identity = 365/594 (61.45%), Postives = 438/594 (73.74%), Query Frame = 1
Query: 20 PAASFRLSGNDPPPPSSSLRRRSRILIISFMSVLLIAASVVSAAILVRSHMTSSQPHFPH 79
P+ + DP L+++++ +I F +L+ VV I S P
Sbjct: 8 PSGPSNPNQKDPATSLPELQKKTKTKLILFTLAVLVVG-VVCFGIFAGIRAVDSGKTEPK 67
Query: 80 --NKPSQAISRACSHTLYPSLCLSSLLSFPGAQSADVHDLVHISLNLTLQHLTKALYSTS 139
KP+QAISR CS +LYP+LC+ +LL FPG+ +AD ++L+HIS N TLQ +KALY++S
Sbjct: 68 LTRKPTQAISRTCSKSLYPNLCIDTLLDFPGSLTADENELIHISFNATLQKFSKALYTSS 127
Query: 140 QIPVLQISKDPLAHSAYEDCMELLNDAIDAFSLSL-----FSKDASNHDIMTWLSAALTY 199
I Q+ P SAY+ C+ELL+D++DA + +L S D S+ D+MTWLS+A+T
Sbjct: 128 TITYTQMP--PRVRSAYDSCLELLDDSVDALTRALSSVVVVSGDESHSDVMTWLSSAMTN 187
Query: 200 HDTCTAGFQDVADLG--VKDEVEAKLSDLSEMISNSLAIFSGFGG--GDLPVENRKRRRL 259
HDTCT GF ++ G VKD+V + DLSEM+SN LAIF+G +PV N R+L
Sbjct: 188 HDTCTDGFDEIEGQGGEVKDQVIGAVKDLSEMVSNCLAIFAGKVKDLSGVPVVNN--RKL 247
Query: 260 MESSTTSWAAENGGDHEGFPAWLSGKDRRLLAAPLSTIQADIVVAKDGSGKFKTVAEAIE 319
+ + T E P WL +DR LL P S IQADI V+KDGSG FKT+AEAI+
Sbjct: 248 LGTEET----------EELPNWLKREDRELLGTPTSAIQADITVSKDGSGTFKTIAEAIK 307
Query: 320 AAPSSSGRRIIIYIKAGKYEEENLKVGRKKTNLMFVGDGKGITVISGGKSVYDKVTTFRT 379
AP S RR +IY+KAG+YEEENLKVGRKKTNLMF+GDGKG TVI+GGKS+ D +TTF T
Sbjct: 308 KAPEHSSRRFVIYVKAGRYEEENLKVGRKKTNLMFIGDGKGKTVITGGKSIADDLTTFHT 367
Query: 380 ATFAGSGTNIILRDMTFENTAGPSKHQAVALRLSADHAVVYHCNIIGYQDTLYVHSNRQF 439
ATFA +G I+RDMTFEN AGP+KHQAVALR+ DHAVVY CNIIGYQD LYVHSNRQF
Sbjct: 368 ATFAATGAGFIVRDMTFENYAGPAKHQAVALRVGGDHAVVYRCNIIGYQDALYVHSNRQF 427
Query: 440 FRECDIYGTIDFIFGNAVVVFQSCNIYARKPMAGQKNTITAQDRKDPNQNTGISIHACKI 499
FREC+IYGT+DFIFGNA V+ QSCNIYARKPMA QK TITAQ+RKDPNQNTGISIHACK+
Sbjct: 428 FRECEIYGTVDFIFGNAAVILQSCNIYARKPMAQQKITITAQNRKDPNQNTGISIHACKL 487
Query: 500 VATGDLEASKGSFPTFLGRPWKLYSRVVYMVSSMGDHIHPRGWLEWQGSFALDTLYYGEY 559
+AT DLEASKGS+PT+LGRPWKLYSRVVYM+S MGDHI PRGWLEW G FALD+LYYGEY
Sbjct: 488 LATPDLEASKGSYPTYLGRPWKLYSRVVYMMSDMGDHIDPRGWLEWNGPFALDSLYYGEY 547
Query: 560 MNSGPGAAVGQRVKWPGYRVITSTVEASKFTVGQFIYGSSWLPSTGVSFSAGLS 603
MN G G+ +GQRVKWPGY VITSTVEASKFTV QFI GSSWLPSTGVSF +GLS
Sbjct: 548 MNKGLGSGIGQRVKWPGYHVITSTVEASKFTVAQFISGSSWLPSTGVSFFSGLS 586
BLAST of Cucsa.202120 vs. TAIR10
Match:
AT3G49220.1 (AT3G49220.1 Plant invertase/pectin methylesterase inhibitor superfamily)
HSP 1 Score: 691.8 bits (1784), Expect = 3.7e-199
Identity = 347/599 (57.93%), Postives = 440/599 (73.46%), Query Frame = 1
Query: 13 SHSANTTPAASFRLSGNDPPPPSSSLRRRSRILIISF-MSVLLIAASVVSAAILVRSHMT 72
S + +TT A P +++ R + ++++ S +++ LI A+ + A + R +
Sbjct: 14 SVTTSTTTAPILNQVSTSEQPENNNRRSKKKLVVSSIVLAISLILAAAIFAGVRSRLKLN 73
Query: 73 SSQPHFPHNKPSQAISRACSHTLYPSLCLSSLLSFPGAQSADVH-DLVHISLNLTLQHLT 132
S P KPSQAIS+AC T +P LC+ SL+ FPG+ +A DL+H+++N+TL H +
Sbjct: 74 QSVPGLAR-KPSQAISKACELTRFPELCVDSLMDFPGSLAASSSKDLIHVTVNMTLHHFS 133
Query: 133 KALYSTSQIPVLQISKDPLAHSAYEDCMELLNDAIDAFSLSL---FSKDASNHDIMTWLS 192
ALYS++ + + + P A SAY+ C+ELL+D++DA S +L S A D+ TWLS
Sbjct: 134 HALYSSASLSFVDMP--PRARSAYDSCVELLDDSVDALSRALSSVVSSSAKPQDVTTWLS 193
Query: 193 AALTYHDTCTAGFQDVADLGVKDEVEAKLSDLSEMISNSLAIFSGFGGGD----LPVENR 252
AALT HDTCT GF V D GVKD + A L +LSE++SN LAIFS GD +P++NR
Sbjct: 194 AALTNHDTCTEGFDGVDDGGVKDHMTAALQNLSELVSNCLAIFSASHDGDDFAGVPIQNR 253
Query: 253 KRRRLMESSTTSWAAENGGDHEGFPAWLSGKDRRLLAAPLSTIQADIVVAKDGSGKFKTV 312
+ + E E FP W+ K+R +L P+S IQADI+V+KDG+G KT+
Sbjct: 254 RLLGVEERE------------EKFPRWMRPKEREILEMPVSQIQADIIVSKDGNGTCKTI 313
Query: 313 AEAIEAAPSSSGRRIIIYIKAGKYEEENLKVGRKKTNLMFVGDGKGITVISGGKSVYDKV 372
+EAI+ AP +S RRIIIY+KAG+YEE NLKVGRKK NLMFVGDGKG TVISGGKS++D +
Sbjct: 314 SEAIKKAPQNSTRRIIIYVKAGRYEENNLKVGRKKINLMFVGDGKGKTVISGGKSIFDNI 373
Query: 373 TTFRTATFAGSGTNIILRDMTFENTAGPSKHQAVALRLSADHAVVYHCNIIGYQDTLYVH 432
TTF TA+FA +G I RD+TFEN AGP+KHQAVALR+ ADHAV+Y CNIIGYQDTLYVH
Sbjct: 374 TTFHTASFAATGAGFIARDITFENWAGPAKHQAVALRIGADHAVIYRCNIIGYQDTLYVH 433
Query: 433 SNRQFFRECDIYGTIDFIFGNAVVVFQSCNIYARKPMAGQKNTITAQDRKDPNQNTGISI 492
SNRQFFRECDIYGT+DFIFGNA VV Q+C+IYARKPM QKNTITAQ+RKDPNQNTGISI
Sbjct: 434 SNRQFFRECDIYGTVDFIFGNAAVVLQNCSIYARKPMDFQKNTITAQNRKDPNQNTGISI 493
Query: 493 HACKIVATGDLEASKGSFPTFLGRPWKLYSRVVYMVSSMGDHIHPRGWLEWQGSFALDTL 552
HA +++A DL+A+ GS T+LGRPWKL+SR VYM+S +G H+H RGWLEW +FALDTL
Sbjct: 494 HASRVLAASDLQATNGSTQTYLGRPWKLFSRTVYMMSYIGGHVHTRGWLEWNTTFALDTL 553
Query: 553 YYGEYMNSGPGAAVGQRVKWPGYRVITSTVEASKFTVGQFIYGSSWLPSTGVSFSAGLS 603
YYGEY+NSGPG+ +GQRV WPGYRVI ST EA++FTV +FIYGSSWLPSTGVSF AGLS
Sbjct: 554 YYGEYLNSGPGSGLGQRVSWPGYRVINSTAEANRFTVAEFIYGSSWLPSTGVSFLAGLS 597
BLAST of Cucsa.202120 vs. TAIR10
Match:
AT3G14310.1 (AT3G14310.1 pectin methylesterase 3)
HSP 1 Score: 474.6 bits (1220), Expect = 9.4e-134
Identity = 260/589 (44.14%), Postives = 364/589 (61.80%), Query Frame = 1
Query: 37 SLRRRSRILIISFMSVLLIAASV--VSAAILVRSHMTSSQPHFPHNKPSQAISRA-CSHT 96
+ ++ +++++S LL A+V +SA + + P S A+ R+ CS T
Sbjct: 13 NFKKNKKLVLLSAAVALLFVAAVAGISAGASKANEKRTLSPS------SHAVLRSSCSST 72
Query: 97 LYPSLCLSSLLSFPGAQSADVHDLVHISLNLTLQHLTKALYSTSQIPVLQISKDPLAHSA 156
YP LC+S++++ G + D++ S+NLT+ + ++ ++ + P +A
Sbjct: 73 RYPELCISAVVTAGGVELTSQKDVIEASVNLTITAVEHNYFTVKKLIKKRKGLTPREKTA 132
Query: 157 YEDCMELLNDAIDAF-----SLSLF--SKDASNH--DIMTWLSAALTYHDTCTAGFQ-DV 216
DC+E +++ +D L L+ K H D+ T +S+A+T +TC GF D
Sbjct: 133 LHDCLETIDETLDELHETVEDLHLYPTKKTLREHAGDLKTLISSAITNQETCLDGFSHDD 192
Query: 217 ADLGVKDEVEAKLSDLSEMISNSLAIFSGFGGGDLP-------VENRKRRRLMESSTTSW 276
AD V+ + + M SN+LA+ D+ + + R+ E+ T+
Sbjct: 193 ADKQVRKALLKGQIHVEHMCSNALAMIKNMTDTDIANFEQKAKITSNNRKLKEENQETTV 252
Query: 277 AAENGG----DHEGFPAWLSGKDRRLLAAPLSTIQADIVVAKDGSGKFKTVAEAIEAAPS 336
A + G D EG+P WLS DRRLL S ++AD VA DGSG FKTVA A+ AAP
Sbjct: 253 AVDIAGAGELDSEGWPTWLSAGDRRLLQG--SGVKADATVAADGSGTFKTVAAAVAAAPE 312
Query: 337 SSGRRIIIYIKAGKYEEENLKVGRKKTNLMFVGDGKGITVISGGKSVYDKVTTFRTATFA 396
+S +R +I+IKAG Y E N++V +KK N+MF+GDG+ T+I+G ++V D TTF +AT A
Sbjct: 313 NSNKRYVIHIKAGVYRE-NVEVAKKKKNIMFMGDGRTRTIITGSRNVVDGSTTFHSATVA 372
Query: 397 GSGTNIILRDMTFENTAGPSKHQAVALRLSADHAVVYHCNIIGYQDTLYVHSNRQFFREC 456
G + RD+TF+NTAGPSKHQAVALR+ +D + Y+C+++ YQDTLYVHSNRQFF +C
Sbjct: 373 AVGERFLARDITFQNTAGPSKHQAVALRVGSDFSAFYNCDMLAYQDTLYVHSNRQFFVKC 432
Query: 457 DIYGTIDFIFGNAVVVFQSCNIYARKPMAGQKNTITAQDRKDPNQNTGISIHACKIVATG 516
I GT+DFIFGNA VV Q C+I+AR+P +GQKN +TAQ R DPNQNTGI I C+I AT
Sbjct: 433 LIAGTVDFIFGNAAVVLQDCDIHARRPNSGQKNMVTAQGRTDPNQNTGIVIQKCRIGATS 492
Query: 517 DLEASKGSFPTFLGRPWKLYSRVVYMVSSMGDHIHPRGWLEWQGSFALDTLYYGEYMNSG 576
DL++ KGSFPT+LGRPWK YS+ V M S++ D I P GW EW G+FAL+TL Y EY N+G
Sbjct: 493 DLQSVKGSFPTYLGRPWKEYSQTVIMQSAISDVIRPEGWSEWTGTFALNTLTYREYSNTG 552
Query: 577 PGAAVGQRVKWPGYRVITSTVEASKFTVGQFIYGSSWLPSTGVSFSAGL 602
GA RVKW G++VIT+ EA K+T GQFI G WL STG FS GL
Sbjct: 553 AGAGTANRVKWRGFKVITAAAEAQKYTAGQFIGGGGWLSSTGFPFSLGL 592
BLAST of Cucsa.202120 vs. TAIR10
Match:
AT1G53830.1 (AT1G53830.1 pectin methylesterase 2)
HSP 1 Score: 473.8 bits (1218), Expect = 1.6e-133
Identity = 263/580 (45.34%), Postives = 350/580 (60.34%), Query Frame = 1
Query: 36 SSLRRRSRILIISFMSVLLIAASVVSAAILVRSHMTSSQPHFPHNKPSQAISRA-CSHTL 95
S + ++++ S LL+ AS+V A ++ +Q + S AI ++ CS TL
Sbjct: 12 SDFKNNKKLILSSAAIALLLLASIVGIAATT-TNQNKNQKITTLSSTSHAILKSVCSSTL 71
Query: 96 YPSLCLSSLLSFPGAQSADVHDLVHISLNLTLQHLTKALYSTSQIPVLQISKDPLAHSAY 155
YP LC S++ + G + +++ SLNLT + + ++ ++ + P +A
Sbjct: 72 YPELCFSAVAATGGKELTSQKEVIEASLNLTTKAVKHNYFAVKKLIAKRKGLTPREVTAL 131
Query: 156 EDCMELLNDAIDAFSLSLFS-------KDASNH--DIMTWLSAALTYHDTCTAGFQ-DVA 215
DC+E +++ +D +++ K H D+ T +S+A+T TC GF D A
Sbjct: 132 HDCLETIDETLDELHVAVEDLHQYPKQKSLRKHADDLKTLISSAITNQGTCLDGFSYDDA 191
Query: 216 DLGVKDEVEAKLSDLSEMISNSLAIFSGFGGGDLP-VENRKRRRLMESSTTSWAAENGGD 275
D V+ + + M SN+LA+ D+ E R + ++ E GD
Sbjct: 192 DRKVRKALLKGQVHVEHMCSNALAMIKNMTETDIANFELRDKSSTFTNNNNRKLKEVTGD 251
Query: 276 --HEGFPAWLSGKDRRLLAAPLSTIQADIVVAKDGSGKFKTVAEAIEAAPSSSGRRIIIY 335
+G+P WLS DRRLL STI+AD VA DGSG F TVA A+ AAP S +R +I+
Sbjct: 252 LDSDGWPKWLSVGDRRLLQG--STIKADATVADDGSGDFTTVAAAVAAAPEKSNKRFVIH 311
Query: 336 IKAGKYEEENLKVGRKKTNLMFVGDGKGITVISGGKSVYDKVTTFRTATFAGSGTNIILR 395
IKAG Y E N++V +KKTN+MF+GDG+G T+I+G ++V D TTF +AT A G + R
Sbjct: 312 IKAGVYRE-NVEVTKKKTNIMFLGDGRGKTIITGSRNVVDGSTTFHSATVAAVGERFLAR 371
Query: 396 DMTFENTAGPSKHQAVALRLSADHAVVYHCNIIGYQDTLYVHSNRQFFRECDIYGTIDFI 455
D+TF+NTAGPSKHQAVALR+ +D + Y C++ YQDTLYVHSNRQFF +C I GT+DFI
Sbjct: 372 DITFQNTAGPSKHQAVALRVGSDFSAFYQCDMFAYQDTLYVHSNRQFFVKCHITGTVDFI 431
Query: 456 FGNAVVVFQSCNIYARKPMAGQKNTITAQDRKDPNQNTGISIHACKIVATGDLEASKGSF 515
FGNA V Q C+I AR+P +GQKN +TAQ R DPNQNTGI I C+I T DL A KG+F
Sbjct: 432 FGNAAAVLQDCDINARRPNSGQKNMVTAQGRSDPNQNTGIVIQNCRIGGTSDLLAVKGTF 491
Query: 516 PTFLGRPWKLYSRVVYMVSSMGDHIHPRGWLEWQGSFALDTLYYGEYMNSGPGAAVGQRV 575
PT+LGRPWK YSR V M S + D I P GW EW GSFALDTL Y EY+N G GA RV
Sbjct: 492 PTYLGRPWKEYSRTVIMQSDISDVIRPEGWHEWSGSFALDTLTYREYLNRGGGAGTANRV 551
Query: 576 KWPGYRVITSTVEASKFTVGQFIYGSSWLPSTGVSFSAGL 602
KW GY+VITS EA FT GQFI G WL STG FS L
Sbjct: 552 KWKGYKVITSDTEAQPFTAGQFIGGGGWLASTGFPFSLSL 587
BLAST of Cucsa.202120 vs. TAIR10
Match:
AT1G11580.1 (AT1G11580.1 methylesterase PCR A)
HSP 1 Score: 420.6 bits (1080), Expect = 1.6e-117
Identity = 247/582 (42.44%), Postives = 340/582 (58.42%), Query Frame = 1
Query: 37 SLRRRSRILIISFMSVLLIAASVVSAAILVRSH------MTSSQP-HFPHNKPSQAISRA 96
SL+ ++ L++SF+++L A + I V ++ +T+SQ H H++ S
Sbjct: 14 SLKHKNLCLVLSFVAILGSVAFFTAQLISVNTNNNDDSLLTTSQICHGAHDQDS------ 73
Query: 97 CSHTL--YPSLCLSSLLSFPGAQSADVHDLVHISLNLTLQHLTKALYSTSQIPVLQISKD 156
C L + +L LS L DL+H+ L ++ L + S+ + S
Sbjct: 74 CQALLSEFTTLSLSKLNRL---------DLLHVFLKNSVWRLESTMTMVSEARIR--SNG 133
Query: 157 PLAHSAYEDCMELLNDAIDAFSLSLFSKDASNHD------IMTWLSAALTYHDTCTAGFQ 216
+ + DC E+++ + D S+ N++ + TWLS+ LT + TC
Sbjct: 134 VRDKAGFADCEEMMDVSKDRMMSSMEELRGGNYNLESYSNVHTWLSSVLTNYMTCLESIS 193
Query: 217 DVADLGVKDEVEAKLSDLSEMISNSLAIFSGFGGGDLPVENRKRRRLMESSTTSWAAENG 276
DV+ + K V+ +L DL +LAIF LP + + +
Sbjct: 194 DVS-VNSKQIVKPQLEDLVSRARVALAIFVSV----LPARDDLKMIISNR---------- 253
Query: 277 GDHEGFPAWLSGKDRRLLAAPLSTIQ--ADIVVAKDGSGKFKTVAEAIEAAPSSSGRRII 336
FP+WL+ DR+LL + T++ A++VVAKDG+GKFKTV EA+ AAP +S R +
Sbjct: 254 -----FPSWLTALDRKLLESSPKTLKVTANVVVAKDGTGKFKTVNEAVAAAPENSNTRYV 313
Query: 337 IYIKAGKYEEENLKVGRKKTNLMFVGDGKGITVISGGKSVYDKVTTFRTATFAGSGTNII 396
IY+K G Y+E + +G+KK NLM VGDGK T+I+G +V D TTFR+AT A +G +
Sbjct: 314 IYVKKGVYKE-TIDIGKKKKNLMLVGDGKDATIITGSLNVIDGSTTFRSATVAANGDGFM 373
Query: 397 LRDMTFENTAGPSKHQAVALRLSADHAVVYHCNIIGYQDTLYVHSNRQFFRECDIYGTID 456
+D+ F+NTAGP+KHQAVALR+SAD V+ C I YQDTLY H+ RQF+R+ I GT+D
Sbjct: 374 AQDIWFQNTAGPAKHQAVALRVSADQTVINRCRIDAYQDTLYTHTLRQFYRDSYITGTVD 433
Query: 457 FIFGNAVVVFQSCNIYARKPMAGQKNTITAQDRKDPNQNTGISIHACKIVATGDLEASKG 516
FIFGN+ VVFQ+C+I AR P AGQKN +TAQ R+D NQNT ISI CKI A+ DL KG
Sbjct: 434 FIFGNSAVVFQNCDIVARNPGAGQKNMLTAQGREDQNQNTAISIQKCKITASSDLAPVKG 493
Query: 517 SFPTFLGRPWKLYSRVVYMVSSMGDHIHPRGWLEWQGSFALDTLYYGEYMNSGPGAAVGQ 576
S TFLGRPWKLYSR V M S + +HI P GW W G FAL TLYYGEY N+GPGA +
Sbjct: 494 SVKTFLGRPWKLYSRTVIMQSFIDNHIDPAGWFPWDGEFALSTLYYGEYANTGPGADTSK 553
Query: 577 RVKWPGYRVITSTVEASKFTVGQFIYGSSWLPSTGVSFSAGL 602
RV W G++VI + EA +FTV + I G WL TGV+F L
Sbjct: 554 RVNWKGFKVIKDSKEAEQFTVAKLIQGGLWLKPTGVTFQEWL 557
BLAST of Cucsa.202120 vs. NCBI nr
Match:
gi|778698893|ref|XP_011654619.1| (PREDICTED: probable pectinesterase/pectinesterase inhibitor 61 [Cucumis sativus])
HSP 1 Score: 1210.7 bits (3131), Expect = 0.0e+00
Identity = 604/604 (100.00%), Postives = 604/604 (100.00%), Query Frame = 1
Query: 1 MNNNNSYNKLHTSHSANTTPAASFRLSGNDPPPPSSSLRRRSRILIISFMSVLLIAASVV 60
MNNNNSYNKLHTSHSANTTPAASFRLSGNDPPPPSSSLRRRSRILIISFMSVLLIAASVV
Sbjct: 1 MNNNNSYNKLHTSHSANTTPAASFRLSGNDPPPPSSSLRRRSRILIISFMSVLLIAASVV 60
Query: 61 SAAILVRSHMTSSQPHFPHNKPSQAISRACSHTLYPSLCLSSLLSFPGAQSADVHDLVHI 120
SAAILVRSHMTSSQPHFPHNKPSQAISRACSHTLYPSLCLSSLLSFPGAQSADVHDLVHI
Sbjct: 61 SAAILVRSHMTSSQPHFPHNKPSQAISRACSHTLYPSLCLSSLLSFPGAQSADVHDLVHI 120
Query: 121 SLNLTLQHLTKALYSTSQIPVLQISKDPLAHSAYEDCMELLNDAIDAFSLSLFSKDASNH 180
SLNLTLQHLTKALYSTSQIPVLQISKDPLAHSAYEDCMELLNDAIDAFSLSLFSKDASNH
Sbjct: 121 SLNLTLQHLTKALYSTSQIPVLQISKDPLAHSAYEDCMELLNDAIDAFSLSLFSKDASNH 180
Query: 181 DIMTWLSAALTYHDTCTAGFQDVADLGVKDEVEAKLSDLSEMISNSLAIFSGFGGGDLPV 240
DIMTWLSAALTYHDTCTAGFQDVADLGVKDEVEAKLSDLSEMISNSLAIFSGFGGGDLPV
Sbjct: 181 DIMTWLSAALTYHDTCTAGFQDVADLGVKDEVEAKLSDLSEMISNSLAIFSGFGGGDLPV 240
Query: 241 ENRKRRRLMESSTTSWAAENGGDHEGFPAWLSGKDRRLLAAPLSTIQADIVVAKDGSGKF 300
ENRKRRRLMESSTTSWAAENGGDHEGFPAWLSGKDRRLLAAPLSTIQADIVVAKDGSGKF
Sbjct: 241 ENRKRRRLMESSTTSWAAENGGDHEGFPAWLSGKDRRLLAAPLSTIQADIVVAKDGSGKF 300
Query: 301 KTVAEAIEAAPSSSGRRIIIYIKAGKYEEENLKVGRKKTNLMFVGDGKGITVISGGKSVY 360
KTVAEAIEAAPSSSGRRIIIYIKAGKYEEENLKVGRKKTNLMFVGDGKGITVISGGKSVY
Sbjct: 301 KTVAEAIEAAPSSSGRRIIIYIKAGKYEEENLKVGRKKTNLMFVGDGKGITVISGGKSVY 360
Query: 361 DKVTTFRTATFAGSGTNIILRDMTFENTAGPSKHQAVALRLSADHAVVYHCNIIGYQDTL 420
DKVTTFRTATFAGSGTNIILRDMTFENTAGPSKHQAVALRLSADHAVVYHCNIIGYQDTL
Sbjct: 361 DKVTTFRTATFAGSGTNIILRDMTFENTAGPSKHQAVALRLSADHAVVYHCNIIGYQDTL 420
Query: 421 YVHSNRQFFRECDIYGTIDFIFGNAVVVFQSCNIYARKPMAGQKNTITAQDRKDPNQNTG 480
YVHSNRQFFRECDIYGTIDFIFGNAVVVFQSCNIYARKPMAGQKNTITAQDRKDPNQNTG
Sbjct: 421 YVHSNRQFFRECDIYGTIDFIFGNAVVVFQSCNIYARKPMAGQKNTITAQDRKDPNQNTG 480
Query: 481 ISIHACKIVATGDLEASKGSFPTFLGRPWKLYSRVVYMVSSMGDHIHPRGWLEWQGSFAL 540
ISIHACKIVATGDLEASKGSFPTFLGRPWKLYSRVVYMVSSMGDHIHPRGWLEWQGSFAL
Sbjct: 481 ISIHACKIVATGDLEASKGSFPTFLGRPWKLYSRVVYMVSSMGDHIHPRGWLEWQGSFAL 540
Query: 541 DTLYYGEYMNSGPGAAVGQRVKWPGYRVITSTVEASKFTVGQFIYGSSWLPSTGVSFSAG 600
DTLYYGEYMNSGPGAAVGQRVKWPGYRVITSTVEASKFTVGQFIYGSSWLPSTGVSFSAG
Sbjct: 541 DTLYYGEYMNSGPGAAVGQRVKWPGYRVITSTVEASKFTVGQFIYGSSWLPSTGVSFSAG 600
Query: 601 LSTN 605
LSTN
Sbjct: 601 LSTN 604
BLAST of Cucsa.202120 vs. NCBI nr
Match:
gi|659074422|ref|XP_008437596.1| (PREDICTED: LOW QUALITY PROTEIN: probable pectinesterase/pectinesterase inhibitor 61 [Cucumis melo])
HSP 1 Score: 1161.0 bits (3002), Expect = 0.0e+00
Identity = 581/605 (96.03%), Postives = 589/605 (97.36%), Query Frame = 1
Query: 1 MNNNNSYNKLHTSHSANTTPAASFRLSGNDPPPPSSSLRRRSRILIISFMSVLLIAASVV 60
MNNNN+Y +LHTSHSANTTPA SFRLSGNDPPPPSSSLRRRSRILIISFMSVLLIAASVV
Sbjct: 1 MNNNNNYKQLHTSHSANTTPATSFRLSGNDPPPPSSSLRRRSRILIISFMSVLLIAASVV 60
Query: 61 SAAILVRSHMTSSQPHFPHNKPSQAISRACSHTLYPSLCLSSLLSFPGAQSADVHDLVHI 120
SAAILVRS + SSQPHFPHNKPSQAISRACSHTLYPSLCLSSLLSFPGAQSADVHDLVHI
Sbjct: 61 SAAILVRSRIASSQPHFPHNKPSQAISRACSHTLYPSLCLSSLLSFPGAQSADVHDLVHI 120
Query: 121 SLNLTLQHLTKALYSTSQIPVLQISKDPLAHSAYEDCMELLNDAIDAFSLSLFSKDASNH 180
SLNLTLQHLTKALYSTSQIP+LQISKDPLAHSAYEDCMELLNDAIDAFSLSLFS ASNH
Sbjct: 121 SLNLTLQHLTKALYSTSQIPILQISKDPLAHSAYEDCMELLNDAIDAFSLSLFSTGASNH 180
Query: 181 DIMTWLSAALTYHDTCTAGFQDVADLGVKDEVEAKLSDLSEMISNSLAIFSGFGGGDLPV 240
DIMTWLSAALTYHDTCT GFQDV DLGVK+EVEAKLSDLSEMISNSLAIFSGFGGGDLPV
Sbjct: 181 DIMTWLSAALTYHDTCTDGFQDVTDLGVKNEVEAKLSDLSEMISNSLAIFSGFGGGDLPV 240
Query: 241 ENRKRRRLMESST-TSWAAENGGDHEGFPAWLSGKDRRLLAAPLSTIQADIVVAKDGSGK 300
ENRKRRRLMES T +SWAAENGGDHEGFP WLSGKDRRLLAAP+S IQADIVVAKDGSGK
Sbjct: 241 ENRKRRRLMESGTPSSWAAENGGDHEGFPEWLSGKDRRLLAAPISAIQADIVVAKDGSGK 300
Query: 301 FKTVAEAIEAAPSSSGRRIIIYIKAGKYEEENLKVGRKKTNLMFVGDGKGITVISGGKSV 360
FKTVAEAIEAAPSSSGRRIIIYIKAGKYEEE LKVGRKKTNLMFVGDGKGITVISGGKSV
Sbjct: 301 FKTVAEAIEAAPSSSGRRIIIYIKAGKYEEEILKVGRKKTNLMFVGDGKGITVISGGKSV 360
Query: 361 YDKVTTFRTATFAGSGTNIILRDMTFENTAGPSKHQAVALRLSADHAVVYHCNIIGYQDT 420
YDKVTTFRTATFAGSGT IILRDM+FENTAGPSKHQAVALRLSADHAVVY CNIIGYQDT
Sbjct: 361 YDKVTTFRTATFAGSGTGIILRDMSFENTAGPSKHQAVALRLSADHAVVYRCNIIGYQDT 420
Query: 421 LYVHSNRQFFRECDIYGTIDFIFGNAVVVFQSCNIYARKPMAGQKNTITAQDRKDPNQNT 480
LYVHSNRQFFRECDIYGTIDFIFGNAVVVFQSCNIYARKPMAGQK TITAQDRKDPNQNT
Sbjct: 421 LYVHSNRQFFRECDIYGTIDFIFGNAVVVFQSCNIYARKPMAGQKXTITAQDRKDPNQNT 480
Query: 481 GISIHACKIVATGDLEASKGSFPTFLGRPWKLYSRVVYMVSSMGDHIHPRGWLEWQGSFA 540
GISIHACKIVATGDLEASKGSFPTFLGRPWKLYSRVVYMVSSMGDHIHPRGWLEWQGSFA
Sbjct: 481 GISIHACKIVATGDLEASKGSFPTFLGRPWKLYSRVVYMVSSMGDHIHPRGWLEWQGSFA 540
Query: 541 LDTLYYGEYMNSGPGAAVGQRVKWPGYRVITSTVEASKFTVGQFIYGSSWLPSTGVSFSA 600
LDTLYYGEYMNSGPGAAVGQRVKWPGYRVITSTVEASKFTVGQFIYGSSWLPSTGVSFSA
Sbjct: 541 LDTLYYGEYMNSGPGAAVGQRVKWPGYRVITSTVEASKFTVGQFIYGSSWLPSTGVSFSA 600
Query: 601 GLSTN 605
GLSTN
Sbjct: 601 GLSTN 605
BLAST of Cucsa.202120 vs. NCBI nr
Match:
gi|1009123309|ref|XP_015878472.1| (PREDICTED: probable pectinesterase/pectinesterase inhibitor 34 [Ziziphus jujuba])
HSP 1 Score: 762.7 bits (1968), Expect = 4.9e-217
Identity = 387/582 (66.49%), Postives = 457/582 (78.52%), Query Frame = 1
Query: 38 LRRRSRILIISFMSVLLIAASVVSAAIL--VRSHMTSSQPHFPHNKPSQAISRACSHTLY 97
+R R +I+ +S +++ LIAAS +SA + VR+ + S H KP+QAISRACS T Y
Sbjct: 32 VRNRKKIIFLSVLAIALIAASAISAVLFIGVRTKASGSPGSVLHRKPTQAISRACSKTRY 91
Query: 98 PSLCLSSLLSFPGAQSADVHDLVHISLNLTLQHLTKALYSTSQIPVLQISKDPLAHSAYE 157
SLC++SLL FPG+ +A DLVHIS N+TLQH++KALY +S I LQ+ DP SAY+
Sbjct: 92 ESLCVNSLLDFPGSLNASEQDLVHISFNMTLQHISKALYLSSGISYLQM--DPHVRSAYD 151
Query: 158 DCMELLNDAIDAFSLSLFS------------KDASNHDIMTWLSAALTYHDTCTAGFQDV 217
DC+ELL D+I+A S SL S S D+MTWLSAALT DTCT GF D+
Sbjct: 152 DCVELLEDSIEALSRSLTSVVPSDGATNRQLSGGSVQDVMTWLSAALTNQDTCTEGFSDL 211
Query: 218 ADLGVKDEVEAKLSDLSEMISNSLAIFSGFGGGD---LPVENRKRRRLMESSTTSWAAEN 277
+ VKD++ +L DLSE++SN LAIF+ D +P++NR RRL+E +
Sbjct: 212 SG-NVKDQMGERLKDLSELVSNCLAIFAASDSDDFSGVPIQNR--RRLLEEDSDISPKNA 271
Query: 278 GGDHEGFPAWLSGKDRRLLAAPLSTIQADIVVAKDGSGKFKTVAEAIEAAPSSSGRRIII 337
GD FP WL KDR+LLA P+S IQADIVV+KDG+G KT+AEAI+ AP SS RR II
Sbjct: 272 AGD---FPRWLGRKDRKLLALPVSEIQADIVVSKDGNGTVKTIAEAIKKAPQSSNRRTII 331
Query: 338 YIKAGKYEEENLKVGRKKTNLMFVGDGKGITVISGGKSVYDKVTTFRTATFAGSGTNIIL 397
Y++AG+YEE+NLKVGRKKTNLMF+GDGKG T+ISGGKSV DK+TTF TA+FA +G I
Sbjct: 332 YVRAGRYEEDNLKVGRKKTNLMFIGDGKGKTIISGGKSVKDKLTTFHTASFAATGAGFIA 391
Query: 398 RDMTFENTAGPSKHQAVALRLSADHAVVYHCNIIGYQDTLYVHSNRQFFRECDIYGTIDF 457
RDMTFEN AGP+KHQAVALR+ ADHAVVY CN+IGYQDTLYVHSNRQFFRECD+YGT+DF
Sbjct: 392 RDMTFENWAGPAKHQAVALRVGADHAVVYRCNVIGYQDTLYVHSNRQFFRECDVYGTVDF 451
Query: 458 IFGNAVVVFQSCNIYARKPMAGQKNTITAQDRKDPNQNTGISIHACKIVATGDLEASKGS 517
IFGNA VVFQ+C+++ARKPMA QKNTITAQ+RKDPNQNTGISIHAC+I+AT DLEASKGS
Sbjct: 452 IFGNAAVVFQNCSMFARKPMAMQKNTITAQNRKDPNQNTGISIHACRILATSDLEASKGS 511
Query: 518 FPTFLGRPWKLYSRVVYMVSSMGDHIHPRGWLEWQGSFALDTLYYGEYMNSGPGAAVGQR 577
F T+LGRPWKLYSR VYM+S MGDHIHPRGWLEW +FALDTLYYGEYMNSGPG AVGQR
Sbjct: 512 FSTYLGRPWKLYSRTVYMLSYMGDHIHPRGWLEWNAAFALDTLYYGEYMNSGPGGAVGQR 571
Query: 578 VKWPGYRVITSTVEASKFTVGQFIYGSSWLPSTGVSFSAGLS 603
VKWPGYRVITSTVEASKFTV QFIYGSSWLPSTGV+F AGLS
Sbjct: 572 VKWPGYRVITSTVEASKFTVAQFIYGSSWLPSTGVAFLAGLS 605
BLAST of Cucsa.202120 vs. NCBI nr
Match:
gi|119507461|dbj|BAF42038.1| (pectin methylesterase 1 [Pyrus communis])
HSP 1 Score: 749.6 bits (1934), Expect = 4.3e-213
Identity = 375/584 (64.21%), Postives = 448/584 (76.71%), Query Frame = 1
Query: 35 SSSLRRRSRILIISFMSVLLIAASVVSAAILVRSHMTSSQPHFP---HNKPSQAISRACS 94
S+S R++++++IS +SV LI AS VSA +L +S H KP++AIS AC+
Sbjct: 35 STSRSRKNKLILISLLSVALILASAVSAVVLFAGRSKASNSAASSALHRKPTKAISDACA 94
Query: 95 HTLYPSLCLSSLLSFPGAQSADVHDLVHISLNLTLQHLTKALYSTSQIPVLQISKDPLAH 154
T +PSLC+ SLL FPG+ +A DLVHIS N+TLQ L+KALY +S + Q+ DP +
Sbjct: 95 TTRFPSLCVDSLLDFPGSTTASEQDLVHISFNMTLQRLSKALYLSSSLSYRQM--DPYSR 154
Query: 155 SAYEDCMELLNDAIDAFSLSLFS---------KDASNHDIMTWLSAALTYHDTCTAGFQD 214
SAY+DC+ELL+D++DA S +L S S D++TWLSAALT DTC GF+
Sbjct: 155 SAYDDCLELLDDSVDALSRALTSVIPGAASTSTSTSTQDVLTWLSAALTNQDTCGEGFEQ 214
Query: 215 VADLGVKDEVEAKLSDLSEMISNSLAIFSGFGGGD---LPVENRKRRRLMESSTT-SWAA 274
+ VK+E++ +L DLSE++SN LAI+S GGGD +P++NR+RR L S
Sbjct: 215 INGGDVKNEMDQRLKDLSELVSNCLAIYSAIGGGDFSGVPIQNRRRRLLGNSDMERDIMG 274
Query: 275 ENGGDHEGFPAWLSGKDRRLLAAPLSTIQADIVVAKDGSGKFKTVAEAIEAAPSSSGRRI 334
+N FP WL ++RRLLA P S IQADI+V+KDGSG FKT+AEAI+ AP SS RR
Sbjct: 275 DNANVSRDFPRWLGRRERRLLAVPGSQIQADIIVSKDGSGTFKTIAEAIKKAPESSSRRT 334
Query: 335 IIYIKAGKYEEENLKVGRKKTNLMFVGDGKGITVISGGKSVYDKVTTFRTATFAGSGTNI 394
IIY+KAG+YEE NLKV RKKTNLMF+GDGKG T+I+GGK+V KVTTF TATFA G
Sbjct: 335 IIYVKAGRYEESNLKVARKKTNLMFIGDGKGKTIITGGKNVAQKVTTFHTATFAAQGAGF 394
Query: 395 ILRDMTFENTAGPSKHQAVALRLSADHAVVYHCNIIGYQDTLYVHSNRQFFRECDIYGTI 454
I RD+TFEN AGP KHQAVALR+ ADHAVVYHC+IIGYQDT YVHSNRQF RE DIYGT+
Sbjct: 395 IARDLTFENYAGPDKHQAVALRIGADHAVVYHCSIIGYQDTFYVHSNRQFVRETDIYGTV 454
Query: 455 DFIFGNAVVVFQSCNIYARKPMAGQKNTITAQDRKDPNQNTGISIHACKIVATGDLEASK 514
DFIFGNA VVFQ C++YARKPMA QKNTITAQ+RKDPNQNTGISIH C+I+AT +LE SK
Sbjct: 455 DFIFGNAAVVFQKCSLYARKPMANQKNTITAQNRKDPNQNTGISIHDCRILATPELEGSK 514
Query: 515 GSFPTFLGRPWKLYSRVVYMVSSMGDHIHPRGWLEWQGSFALDTLYYGEYMNSGPGAAVG 574
GSFPT+LGRPWKLYSR VYM+S +GDH+HPRGWLEW G+FALDTLYYGEYMNSGPGAAVG
Sbjct: 515 GSFPTYLGRPWKLYSRTVYMLSFIGDHVHPRGWLEWNGNFALDTLYYGEYMNSGPGAAVG 574
Query: 575 QRVKWPGYRVITSTVEASKFTVGQFIYGSSWLPSTGVSFSAGLS 603
QRV WPGYRVITS VEA KFTV QFIYGSSWLPSTGV+F AGLS
Sbjct: 575 QRVTWPGYRVITSPVEAGKFTVAQFIYGSSWLPSTGVAFLAGLS 616
BLAST of Cucsa.202120 vs. NCBI nr
Match:
gi|694345742|ref|XP_009357300.1| (PREDICTED: probable pectinesterase/pectinesterase inhibitor 34 [Pyrus x bretschneideri])
HSP 1 Score: 748.8 bits (1932), Expect = 7.3e-213
Identity = 375/584 (64.21%), Postives = 447/584 (76.54%), Query Frame = 1
Query: 35 SSSLRRRSRILIISFMSVLLIAASVVSAAILVRSHMTSSQPHFP---HNKPSQAISRACS 94
S+S R++++++IS +SV LI AS VSA +L +S H KP++AIS AC+
Sbjct: 35 STSRSRKNKLILISLLSVALILASAVSAVVLFAGRSKASNSAASSALHRKPTKAISDACA 94
Query: 95 HTLYPSLCLSSLLSFPGAQSADVHDLVHISLNLTLQHLTKALYSTSQIPVLQISKDPLAH 154
T +PSLC+ SLL FPG+ +A DLVHIS N+TLQ L+KALY +S + Q+ DP +
Sbjct: 95 RTRFPSLCVDSLLDFPGSTTASEQDLVHISFNMTLQRLSKALYLSSSLSYRQM--DPYSR 154
Query: 155 SAYEDCMELLNDAIDAFSLSLFS---------KDASNHDIMTWLSAALTYHDTCTAGFQD 214
SAY+DC+ELL+D++DA S +L S S D++TWLSAALT DTC GF+
Sbjct: 155 SAYDDCLELLDDSVDALSRALTSVIPGAASTSTSTSTQDVLTWLSAALTNQDTCGEGFEQ 214
Query: 215 VADLGVKDEVEAKLSDLSEMISNSLAIFSGFGGGD---LPVENRKRRRLMESSTT-SWAA 274
+ VK+E+ +L DLSE++SN LAI+S GGGD +P++NR+RR L S
Sbjct: 215 INGGDVKNEMGQRLKDLSELVSNCLAIYSAIGGGDFSGVPIQNRRRRLLGNSDMERDIMG 274
Query: 275 ENGGDHEGFPAWLSGKDRRLLAAPLSTIQADIVVAKDGSGKFKTVAEAIEAAPSSSGRRI 334
+N FP WL ++RRLLA P S IQADI+V+KDGSG FKT+AEAI+ AP SS RR
Sbjct: 275 DNANVSRDFPRWLGRRERRLLAVPGSQIQADIIVSKDGSGTFKTIAEAIKKAPESSSRRT 334
Query: 335 IIYIKAGKYEEENLKVGRKKTNLMFVGDGKGITVISGGKSVYDKVTTFRTATFAGSGTNI 394
IIY+KAG+YEE NLKV RKKTNLMF+GDGKG T+I+GGK+V KVTTF TATFA G
Sbjct: 335 IIYVKAGRYEESNLKVARKKTNLMFIGDGKGKTIITGGKNVAQKVTTFHTATFAAQGAGF 394
Query: 395 ILRDMTFENTAGPSKHQAVALRLSADHAVVYHCNIIGYQDTLYVHSNRQFFRECDIYGTI 454
I RD+TFEN AGP KHQAVALR+ ADHAVVYHC+IIGYQDT YVHSNRQF RE DIYGT+
Sbjct: 395 IARDLTFENYAGPDKHQAVALRIGADHAVVYHCSIIGYQDTFYVHSNRQFVRETDIYGTV 454
Query: 455 DFIFGNAVVVFQSCNIYARKPMAGQKNTITAQDRKDPNQNTGISIHACKIVATGDLEASK 514
DFIFGNA VVFQ C++YARKPMA QKNTITAQ+RKDPNQNTGISIH C+I+AT +LE SK
Sbjct: 455 DFIFGNAAVVFQKCSLYARKPMANQKNTITAQNRKDPNQNTGISIHDCRILATPELEGSK 514
Query: 515 GSFPTFLGRPWKLYSRVVYMVSSMGDHIHPRGWLEWQGSFALDTLYYGEYMNSGPGAAVG 574
GSFPT+LGRPWKLYSR VYM+S +GDH+HPRGWLEW G+FALDTLYYGEYMNSGPGAAVG
Sbjct: 515 GSFPTYLGRPWKLYSRTVYMLSFIGDHVHPRGWLEWNGNFALDTLYYGEYMNSGPGAAVG 574
Query: 575 QRVKWPGYRVITSTVEASKFTVGQFIYGSSWLPSTGVSFSAGLS 603
QRV WPGYRVITS VEA KFTV QFIYGSSWLPSTGV+F AGLS
Sbjct: 575 QRVTWPGYRVITSPVEAGKFTVAQFIYGSSWLPSTGVAFLAGLS 616
The following BLAST results are available for this feature:
| Match Name | E-value | Identity | Description | |
|---|
| PME61_ARATH | 3.1e-203 | 61.45 | Probable pectinesterase/pectinesterase inhibitor 61 OS=Arabidopsis thaliana GN=P... | [more] |
| PME34_ARATH | 6.6e-198 | 57.93 | Probable pectinesterase/pectinesterase inhibitor 34 OS=Arabidopsis thaliana GN=P... | [more] |
| PME3_ARATH | 1.7e-132 | 44.14 | Pectinesterase/pectinesterase inhibitor 3 OS=Arabidopsis thaliana GN=PME3 PE=2 S... | [more] |
| PME2_ARATH | 2.8e-132 | 45.34 | Pectinesterase 2 OS=Arabidopsis thaliana GN=PME2 PE=2 SV=2 | [more] |
| PME3_CITSI | 1.4e-131 | 44.67 | Pectinesterase 3 OS=Citrus sinensis PE=1 SV=1 | [more] |
| Match Name | E-value | Identity | Description | |
|---|
| A0A0A0KJG1_CUCSA | 0.0e+00 | 100.00 | Pectinesterase OS=Cucumis sativus GN=Csa_5G139430 PE=4 SV=1 | [more] |
| A0ZNJ9_PYRCO | 3.0e-213 | 64.21 | Pectinesterase OS=Pyrus communis GN=PcPME1 PE=2 SV=1 | [more] |
| V4TRH7_9ROSI | 7.4e-212 | 63.82 | Pectinesterase OS=Citrus clementina GN=CICLE_v10019376mg PE=4 SV=1 | [more] |
| W9RDL0_9ROSA | 2.4e-210 | 63.12 | Pectinesterase OS=Morus notabilis GN=L484_006819 PE=4 SV=1 | [more] |
| A0A067JU95_JATCU | 3.1e-210 | 61.87 | Pectinesterase OS=Jatropha curcas GN=JCGZ_20123 PE=4 SV=1 | [more] |
