CmoCh18G013460 (gene) Cucurbita moschata (Rifu)

BLAST of CmoCh18G013460 vs. Swiss-Prot
Match: PME34_ARATH (Probable pectinesterase/pectinesterase inhibitor 34 OS=Arabidopsis thaliana GN=PME34 PE=2 SV=1)

HSP 1 Score: 753.8 bits (1945), Expect = 1.4e-216
Identity = 386/564 (68.44%), Postives = 451/564 (79.96%), Query Frame = 1

Query: 21  SVLSLLSLILILPAVSPTSLLGLRTSSDPKLPRKPTRAISKACARSRLPALCVDSLLDFP 80
           S++  +SLIL     +          S P L RKP++AISKAC  +R P LCVDSL+DFP
Sbjct: 49  SIVLAISLILAAAIFAGVRSRLKLNQSVPGLARKPSQAISKACELTRFPELCVDSLMDFP 108

Query: 81  GSLSANE-RDLVHISFNLTLHHLNEALYSSSHLSSLHMDLPLRSAYDACLELLDDSVHAL 140
           GSL+A+  +DL+H++ N+TLHH + ALYSS+ LS + M    RSAYD+C+ELLDDSV AL
Sbjct: 109 GSLAASSSKDLIHVTVNMTLHHFSHALYSSASLSFVDMPPRARSAYDSCVELLDDSVDAL 168

Query: 141 TRSFHSLAPPSSDSRPTPLASSSDDVITWLSAALTNQDTCTDGFSQLS-GNVKDQMTDKL 200
           +R+  S+   SS ++P        DV TWLSAALTN DTCT+GF  +  G VKD MT  L
Sbjct: 169 SRALSSVV--SSSAKP-------QDVTTWLSAALTNHDTCTEGFDGVDDGGVKDHMTAAL 228

Query: 201 HNLSELVSNCLALFSGSVR-NDFSGVPIQNKRRLMNAAEKEDDSGNFPGWMSRRERRLLT 260
            NLSELVSNCLA+FS S   +DF+GVPIQN RRL+   E+E+    FP WM  +ER +L 
Sbjct: 229 QNLSELVSNCLAIFSASHDGDDFAGVPIQN-RRLLGVEEREE---KFPRWMRPKEREILE 288

Query: 261 LPVGALQADIVVSKDGNGTVKTIAEAIKKAPEFSTRKTIIYVMAGRYEEKNLKVGRKKTN 320
           +PV  +QADI+VSKDGNGT KTI+EAIKKAP+ STR+ IIYV AGRYEE NLKVGRKK N
Sbjct: 289 MPVSQIQADIIVSKDGNGTCKTISEAIKKAPQNSTRRIIIYVKAGRYEENNLKVGRKKIN 348

Query: 321 LMFVGDGKGKTVISGGKSIFDNVTTFHTASFAVSGAGIILRDMTFVNWAGPARHQAVALR 380
           LMFVGDGKGKTVISGGKSIFDN+TTFHTASFA +GAG I RD+TF NWAGPA+HQAVALR
Sbjct: 349 LMFVGDGKGKTVISGGKSIFDNITTFHTASFAATGAGFIARDITFENWAGPAKHQAVALR 408

Query: 381 VSADHAVVYRCNVIGYQDTLYVHSNRQFFRECDIYGTVDFIFGNAAVVFQNCSIYARKAM 440
           + ADHAV+YRCN+IGYQDTLYVHSNRQFFRECDIYGTVDFIFGNAAVV QNCSIYARK M
Sbjct: 409 IGADHAVIYRCNIIGYQDTLYVHSNRQFFRECDIYGTVDFIFGNAAVVLQNCSIYARKPM 468

Query: 441 ELQKNTITAQNRKDPNQNTGISIHACRILATPDLASSSTATNPTYLGRPWKLYSRVVYMQ 500
           + QKNTITAQNRKDPNQNTGISIHA R+LA  DL +++ +T  TYLGRPWKL+SR VYM 
Sbjct: 469 DFQKNTITAQNRKDPNQNTGISIHASRVLAASDLQATNGSTQ-TYLGRPWKLFSRTVYMM 528

Query: 501 SYMGGHVHPRGWLEWNATFALDTLYYGEYMNYGPGGAVGQRVTWPGYRVITSRVEASQFT 560
           SY+GGHVH RGWLEWN TFALDTLYYGEY+N GPG  +GQRV+WPGYRVI S  EA++FT
Sbjct: 529 SYIGGHVHTRGWLEWNTTFALDTLYYGEYLNSGPGSGLGQRVSWPGYRVINSTAEANRFT 588

Query: 561 VAQFIYGSSWLPSTGVAFLGGLNL 582
           VA+FIYGSSWLPSTGV+FL GL++
Sbjct: 589 VAEFIYGSSWLPSTGVSFLAGLSI 598

BLAST of CmoCh18G013460 vs. Swiss-Prot
Match: PME61_ARATH (Probable pectinesterase/pectinesterase inhibitor 61 OS=Arabidopsis thaliana GN=PME61 PE=2 SV=1)

HSP 1 Score: 729.9 bits (1883), Expect = 2.1e-209
Identity = 378/599 (63.11%), Postives = 455/599 (75.96%), Query Frame = 1

Query: 1   MGCRRLNETHPAANPRRGPLSVLSLLS------LILILPAVSPTSLL------GLRT--- 60
           MG  RL  + P+   ++ P + L  L       LIL   AV    ++      G+R    
Sbjct: 1   MGYDRLGPSGPSNPNQKDPATSLPELQKKTKTKLILFTLAVLVVGVVCFGIFAGIRAVDS 60

Query: 61  -SSDPKLPRKPTRAISKACARSRLPALCVDSLLDFPGSLSANERDLVHISFNLTLHHLNE 120
             ++PKL RKPT+AIS+ C++S  P LC+D+LLDFPGSL+A+E +L+HISFN TL   ++
Sbjct: 61  GKTEPKLTRKPTQAISRTCSKSLYPNLCIDTLLDFPGSLTADENELIHISFNATLQKFSK 120

Query: 121 ALYSSSHLSSLHMDLPLRSAYDACLELLDDSVHALTRSFHSLAPPSSDSRPTPLASSSDD 180
           ALY+SS ++   M   +RSAYD+CLELLDDSV ALTR+  S+   S D        S  D
Sbjct: 121 ALYTSSTITYTQMPPRVRSAYDSCLELLDDSVDALTRALSSVVVVSGDE-------SHSD 180

Query: 181 VITWLSAALTNQDTCTDGFSQLSGN---VKDQMTDKLHNLSELVSNCLALFSGSVRNDFS 240
           V+TWLS+A+TN DTCTDGF ++ G    VKDQ+   + +LSE+VSNCLA+F+G V+ D S
Sbjct: 181 VMTWLSSAMTNHDTCTDGFDEIEGQGGEVKDQVIGAVKDLSEMVSNCLAIFAGKVK-DLS 240

Query: 241 GVPIQNKRRLMNAAEKEDDSGNFPGWMSRRERRLLTLPVGALQADIVVSKDGNGTVKTIA 300
           GVP+ N R+L+   E E+     P W+ R +R LL  P  A+QADI VSKDG+GT KTIA
Sbjct: 241 GVPVVNNRKLLGTEETEE----LPNWLKREDRELLGTPTSAIQADITVSKDGSGTFKTIA 300

Query: 301 EAIKKAPEFSTRKTIIYVMAGRYEEKNLKVGRKKTNLMFVGDGKGKTVISGGKSIFDNVT 360
           EAIKKAPE S+R+ +IYV AGRYEE+NLKVGRKKTNLMF+GDGKGKTVI+GGKSI D++T
Sbjct: 301 EAIKKAPEHSSRRFVIYVKAGRYEEENLKVGRKKTNLMFIGDGKGKTVITGGKSIADDLT 360

Query: 361 TFHTASFAVSGAGIILRDMTFVNWAGPARHQAVALRVSADHAVVYRCNVIGYQDTLYVHS 420
           TFHTA+FA +GAG I+RDMTF N+AGPA+HQAVALRV  DHAVVYRCN+IGYQD LYVHS
Sbjct: 361 TFHTATFAATGAGFIVRDMTFENYAGPAKHQAVALRVGGDHAVVYRCNIIGYQDALYVHS 420

Query: 421 NRQFFRECDIYGTVDFIFGNAAVVFQNCSIYARKAMELQKNTITAQNRKDPNQNTGISIH 480
           NRQFFREC+IYGTVDFIFGNAAV+ Q+C+IYARK M  QK TITAQNRKDPNQNTGISIH
Sbjct: 421 NRQFFRECEIYGTVDFIFGNAAVILQSCNIYARKPMAQQKITITAQNRKDPNQNTGISIH 480

Query: 481 ACRILATPDLASSSTATNPTYLGRPWKLYSRVVYMQSYMGGHVHPRGWLEWNATFALDTL 540
           AC++LATPDL  +S  + PTYLGRPWKLYSRVVYM S MG H+ PRGWLEWN  FALD+L
Sbjct: 481 ACKLLATPDL-EASKGSYPTYLGRPWKLYSRVVYMMSDMGDHIDPRGWLEWNGPFALDSL 540

Query: 541 YYGEYMNYGPGGAVGQRVTWPGYRVITSRVEASQFTVAQFIYGSSWLPSTGVAFLGGLN 581
           YYGEYMN G G  +GQRV WPGY VITS VEAS+FTVAQFI GSSWLPSTGV+F  GL+
Sbjct: 541 YYGEYMNKGLGSGIGQRVKWPGYHVITSTVEASKFTVAQFISGSSWLPSTGVSFFSGLS 586

BLAST of CmoCh18G013460 vs. Swiss-Prot
Match: PME_PRUPE (Pectinesterase/pectinesterase inhibitor PPE8B OS=Prunus persica PE=2 SV=1)

HSP 1 Score: 427.9 bits (1099), Expect = 1.7e-118
Identity = 219/466 (47.00%), Postives = 294/466 (63.09%), Query Frame = 1

Query: 110 SHLSSLHMDLPLRSAYDACLELLDDSVHALTRSFHSLAPPSSDSRPTPLASSSDDVITWL 169
           S  ++   D  L +A   CL+LLD S   L  S  +       +  T   SS  D+ TWL
Sbjct: 75  SQFANAFGDFRLANAISDCLDLLDFSADELNWSLSASQNQKGKNNSTGKLSS--DLRTWL 134

Query: 170 SAALTNQDTCTDGFSQLSGNVKDQMTDKLHNLSELVSNCLALFSGSVRNDFSGVPIQNKR 229
           SAAL NQDTC++GF   +  V+  ++  L  ++ LV   L                    
Sbjct: 135 SAALVNQDTCSNGFEGTNSIVQGLISAGLGQVTSLVQELLT------------------- 194

Query: 230 RLMNAAEKEDDSGNFPGWMSRRERRLLTLPVGALQADIVVSKDGNGTVKTIAEAIKKAPE 289
           ++   + ++  +G  P W+  ++R+LL      +  D +V++DG G    + +A+  AP+
Sbjct: 195 QVHPNSNQQGPNGQIPSWVKTKDRKLLQ--ADGVSVDAIVAQDGTGNFTNVTDAVLAAPD 254

Query: 290 FSTRKTIIYVMAGRYEEKNLKVGRKKTNLMFVGDGKGKTVISGGKSIFDNVTTFHTASFA 349
           +S R+ +IY+  G Y+E N+++ +KK NLM +GDG   T+ISG +S  D  TTF +A+FA
Sbjct: 255 YSMRRYVIYIKRGTYKE-NVEIKKKKWNLMMIGDGMDATIISGNRSFVDGWTTFRSATFA 314

Query: 350 VSGAGIILRDMTFVNWAGPARHQAVALRVSADHAVVYRCNVIGYQDTLYVHSNRQFFREC 409
           VSG G I RD+TF N AGP +HQAVALR  +D +V YRCN+ GYQDTLY H+ RQF+R+C
Sbjct: 315 VSGRGFIARDITFENTAGPEKHQAVALRSDSDLSVFYRCNIRGYQDTLYTHTMRQFYRDC 374

Query: 410 DIYGTVDFIFGNAAVVFQNCSIYARKAMELQKNTITAQNRKDPNQNTGISIHACRILATP 469
            I GTVDFIFG+A VVFQNC I A+K +  QKN+ITAQ RKDPN+ TGISI  C I A  
Sbjct: 375 KISGTVDFIFGDATVVFQNCQILAKKGLPNQKNSITAQGRKDPNEPTGISIQFCNITADS 434

Query: 470 DLASSSTATNPTYLGRPWKLYSRVVYMQSYMGGHVHPRGWLEWNATFALDTLYYGEYMNY 529
           DL ++S  + PTYLGRPWKLYSR V MQS++   + P GWLEWN  FAL++L+YGEYMNY
Sbjct: 435 DLEAASVNSTPTYLGRPWKLYSRTVIMQSFLSNVIRPEGWLEWNGDFALNSLFYGEYMNY 494

Query: 530 GPGGAVGQRVTWPGYRVITSRVEASQFTVAQFIYGSSWLPSTGVAF 576
           GPG  +G RV WPGY+V     +A  +TVAQFI G+ WLPSTGV +
Sbjct: 495 GPGAGLGSRVKWPGYQVFNESTQAKNYTVAQFIEGNLWLPSTGVKY 516

BLAST of CmoCh18G013460 vs. Swiss-Prot
Match: PME32_ARATH (Probable pectinesterase/pectinesterase inhibitor 32 OS=Arabidopsis thaliana GN=PME32 PE=2 SV=1)

HSP 1 Score: 411.8 bits (1057), Expect = 1.3e-113
Identity = 216/452 (47.79%), Postives = 281/452 (62.17%), Query Frame = 1

Query: 128 CLELLDDSVHALTRSFHSLAPPSSDSRPTPLASSSDDVITWLSAALTNQDTCTDGFSQLS 187
           C++LLD +   L+    +   P+     T    S  D+ TW+SAAL+NQDTC DGF   +
Sbjct: 84  CVDLLDSAAEELSWIISASQSPNGKDNSTGDVGS--DLRTWISAALSNQDTCLDGFEGTN 143

Query: 188 GNVKDQMTDKLHNLSELVSNCLALFSGSVRNDFSGVPIQNKRRLMNAAEKEDDSGNFPGW 247
           G +K  +   L  +   V N L +            P     +     +       FP W
Sbjct: 144 GIIKKIVAGGLSKVGTTVRNLLTMVHSP-----PSKPKPKPIKAQTMTKAHSGFSKFPSW 203

Query: 248 MSRRERRLLTLPVGALQADIVVSKDGNGTVKTIAEAIKKAPEFSTRKTIIYVMAGRYEEK 307
           +   +R+LL        AD VV+ DG G   TI++A+  AP++ST++ +I+V  G Y E 
Sbjct: 204 VKPGDRKLLQTD-NITVADAVVAADGTGNFTTISDAVLAAPDYSTKRYVIHVKRGVYVE- 263

Query: 308 NLKVGRKKTNLMFVGDGKGKTVISGGKSIFDNVTTFHTASFAVSGAGIILRDMTFVNWAG 367
           N+++ +KK N+M VGDG   TVI+G +S  D  TTF +A+FAVSG G I RD+TF N AG
Sbjct: 264 NVEIKKKKWNIMMVGDGIDATVITGNRSFIDGWTTFRSATFAVSGRGFIARDITFQNTAG 323

Query: 368 PARHQAVALRVSADHAVVYRCNVIGYQDTLYVHSNRQFFRECDIYGTVDFIFGNAAVVFQ 427
           P +HQAVA+R   D  V YRC + GYQDTLY HS RQFFREC I GTVDFIFG+A  VFQ
Sbjct: 324 PEKHQAVAIRSDTDLGVFYRCAMRGYQDTLYAHSMRQFFRECIITGTVDFIFGDATAVFQ 383

Query: 428 NCSIYARKAMELQKNTITAQNRKDPNQNTGISIHACRILATPDLASSSTATNPTYLGRPW 487
           +C I A++ +  QKN+ITAQ RKDPN+ TG +I    I A  DL  +   T  TYLGRPW
Sbjct: 384 SCQIKAKQGLPNQKNSITAQGRKDPNEPTGFTIQFSNIAADTDLLLNLN-TTATYLGRPW 443

Query: 488 KLYSRVVYMQSYMGGHVHPRGWLEWNATFALDTLYYGEYMNYGPGGAVGQRVTWPGYRVI 547
           KLYSR V+MQ+YM   ++P GWLEWN  FALDTLYYGEYMN GPG ++ +RV WPGY V+
Sbjct: 444 KLYSRTVFMQNYMSDAINPVGWLEWNGNFALDTLYYGEYMNSGPGASLDRRVKWPGYHVL 503

Query: 548 TSRVEASQFTVAQFIYGSSWLPSTGVAFLGGL 580
            +  EA+ FTV+Q I G+ WLPSTG+ F+ GL
Sbjct: 504 NTSAEANNFTVSQLIQGNLWLPSTGITFIAGL 525

BLAST of CmoCh18G013460 vs. Swiss-Prot
Match: PME3_CITSI (Pectinesterase 3 OS=Citrus sinensis PE=1 SV=1)

HSP 1 Score: 411.8 bits (1057), Expect = 1.3e-113
Identity = 242/574 (42.16%), Postives = 341/574 (59.41%), Query Frame = 1

Query: 15  PRRGPLSVLSLLSLILILPAVSP--TSLLGLRTSSDPKLPRKPTRAISKA-CARSRLPAL 74
           P++     L+L + +L++ AV      +   + S D     +P  AI K+ C+ +R P L
Sbjct: 24  PKKKKKLFLALFATLLVVAAVIGIVAGVNSRKNSGDNG--NEPHHAILKSSCSSTRYPDL 83

Query: 75  CVDSLLDFPGSLS--ANERDLVHISFNLTLHHLNEALYSSSHL-SSLHMDLPLRSAYDAC 134
           C  ++   P +     +++D++ +S N+T   +    +    L    ++    + A   C
Sbjct: 84  CFSAIAAVPEASKKVTSQKDVIEMSLNITTTAVEHNYFGIQKLLKRTNLTKREKVALHDC 143

Query: 135 LELLDDSVHALTRSFHSLAP-PSSDSRPTPLASSSDDVITWLSAALTNQDTCTDGFSQLS 194
           LE +D+++  L ++   L   P+  S    L+  +DD+ T +SAA+TNQ TC DGFS   
Sbjct: 144 LETIDETLDELHKAVEDLEEYPNKKS----LSQHADDLKTLMSAAMTNQGTCLDGFSHDD 203

Query: 195 GN--VKDQMTDKLHNLSELVSNCLALFSGSVRNDFSGVPIQNKRRLMNAAEKEDDSGNFP 254
            N  V+D ++D   ++ ++ SN LA+       D   +   N R+L+      D    +P
Sbjct: 204 ANKHVRDALSDGQVHVEKMCSNALAMIKNMTDTDMMIMRTSNNRKLIEETSTVD---GWP 263

Query: 255 GWMSRRERRLLTLPVGALQADIVVSKDGNGTVKTIAEAIKKAPEFSTRKTIIYVMAGRYE 314
            W+S  +RRLL     ++  ++VV+ DG+G  KT+A ++  AP+  T++ II + AG Y 
Sbjct: 264 AWLSTGDRRLLQS--SSVTPNVVVAADGSGNFKTVAASVAAAPQGGTKRYIIRIKAGVYR 323

Query: 315 EKNLKVGRKKTNLMFVGDGKGKTVISGGKSIFDNVTTFHTASFAVSGAGIILRDMTFVNW 374
           E N++V +K  N+MF+GDG+ +T+I+G +++ D  TTF +A+ AV G G + RD+TF N 
Sbjct: 324 E-NVEVTKKHKNIMFIGDGRTRTIITGSRNVVDGSTTFKSATVAVVGEGFLARDITFQNT 383

Query: 375 AGPARHQAVALRVSADHAVVYRCNVIGYQDTLYVHSNRQFFRECDIYGTVDFIFGNAAVV 434
           AGP++HQAVALRV AD +  Y C+++ YQDTLYVHSNRQFF  C I GTVDFIFGNAA V
Sbjct: 384 AGPSKHQAVALRVGADLSAFYNCDMLAYQDTLYVHSNRQFFVNCLIAGTVDFIFGNAAAV 443

Query: 435 FQNCSIYARKAMELQKNTITAQNRKDPNQNTGISIHACRILATPDLASSSTATNPTYLGR 494
            QNC I+ARK    QKN +TAQ R DPNQNTGI I   RI AT DL      + PTYLGR
Sbjct: 444 LQNCDIHARKPNSGQKNMVTAQGRADPNQNTGIVIQKSRIGATSDL-KPVQGSFPTYLGR 503

Query: 495 PWKLYSRVVYMQSYMGGHVHPRGWLEWNATFALDTLYYGEYMNYGPGGAVGQRVTWPGYR 554
           PWK YSR V MQS +   +HP GW EW+  FAL+TL+YGE+ N G G     RV W G+R
Sbjct: 504 PWKEYSRTVIMQSSITDVIHPAGWHEWDGNFALNTLFYGEHQNAGAGAGTSGRVKWKGFR 563

Query: 555 VITSRVEASQFTVAQFIYGSSWLPSTGVAFLGGL 580
           VITS  EA  FT   FI GSSWL STG  F  GL
Sbjct: 564 VITSATEAQAFTPGSFIAGSSWLGSTGFPFSLGL 584

BLAST of CmoCh18G013460 vs. TrEMBL
Match: A0A0A0L6S0_CUCSA (Pectinesterase OS=Cucumis sativus GN=Csa_3G239880 PE=4 SV=1)

HSP 1 Score: 941.0 bits (2431), Expect = 6.7e-271
Identity = 478/583 (81.99%), Postives = 521/583 (89.37%), Query Frame = 1

Query: 5   RLNETHPAANPRRGPLSVLSLLSLILILPA-VSPTSLLGLRTSS----DPKLPRKPTRAI 64
           +L+  HP  N R   L ++S LSL+LI+ A VS T L+GLRT+S    DP LPRKPT+AI
Sbjct: 25  QLSSHHPTPNRRTTRLLLISFLSLLLIVAAAVSATFLIGLRTASPARSDPNLPRKPTQAI 84

Query: 65  SKACARSRLPALCVDSLLDFPGSLSANERDLVHISFNLTLHHLNEALYSSSHLSSLHMDL 124
           SKAC+R+R P LCV+SLLDFPGSL+ANE+DLVHISFN+TL HLN+ALYSSS +SSL MDL
Sbjct: 85  SKACSRTRFPTLCVNSLLDFPGSLNANEQDLVHISFNVTLQHLNKALYSSSEISSLQMDL 144

Query: 125 PLRSAYDACLELLDDSVHALTRSFHSLAPPSSDSRPTPLASSSDDVITWLSAALTNQDTC 184
            +RSAYDACLELLDDS+ A++RS  S+AP SS S P  L SS +D+ITWLSAALTNQDTC
Sbjct: 145 RVRSAYDACLELLDDSIDAVSRSLQSVAPSSSSSTPQRLGSS-EDIITWLSAALTNQDTC 204

Query: 185 TDGFSQLSGNVKDQMTDKLHNLSELVSNCLALFSGSVRNDFSGVPIQNKRRLMNA-AEKE 244
           TDGFS+LSG VK+QM DKLHNLSELVSNCLALFSGS  +DF+GVPIQNKRRLM    E E
Sbjct: 205 TDGFSELSGTVKNQMADKLHNLSELVSNCLALFSGSETSDFAGVPIQNKRRLMEEEGENE 264

Query: 245 DDSGNFPGWMSRRERRLLTLPVGALQADIVVSKDGNGTVKTIAEAIKKAPEFSTRKTIIY 304
           D SG FP WM+RRERRLLTLPVG LQADIVVS++GNGTVKTIAEAIKKAP++S+R+TIIY
Sbjct: 265 DSSGKFPRWMNRRERRLLTLPVGVLQADIVVSQNGNGTVKTIAEAIKKAPQYSSRRTIIY 324

Query: 305 VMAGRYEEKNLKVGRKKTNLMFVGDGKGKTVISGGKSIFDNVTTFHTASFAVSGAGIILR 364
           VMAGRYEEKNLKVGRKKTNLMFVGDGKGKTVISG KSIFDNVTTFHTASFA +GAGIILR
Sbjct: 325 VMAGRYEEKNLKVGRKKTNLMFVGDGKGKTVISGSKSIFDNVTTFHTASFAATGAGIILR 384

Query: 365 DMTFVNWAGPARHQAVALRVSADHAVVYRCNVIGYQDTLYVHSNRQFFRECDIYGTVDFI 424
           DMTF NWAGP RHQAVALRV ADHAVVYRCN+IGYQDTLYVHSNRQF+RECDIYGTVDFI
Sbjct: 385 DMTFENWAGPGRHQAVALRVGADHAVVYRCNIIGYQDTLYVHSNRQFYRECDIYGTVDFI 444

Query: 425 FGNAAVVFQNCSIYARKAMELQKNTITAQNRKDPNQNTGISIHACRILATPDLASSSTAT 484
           FGNAAVVFQNCSIYARKAM LQKNTITAQNRKDPNQNTGISIHACRILAT DL SS+T +
Sbjct: 445 FGNAAVVFQNCSIYARKAMALQKNTITAQNRKDPNQNTGISIHACRILATSDLESSNT-S 504

Query: 485 NPTYLGRPWKLYSRVVYMQSYMGGHVHPRGWLEWNATFALDTLYYGEYMNYGPGGAVGQR 544
           NPTYLGRPWKLYSR V M SY+G HVHPRGWLEWNATFALDTLYYGEYMNYGPGGAVGQR
Sbjct: 505 NPTYLGRPWKLYSRTVVMLSYIGNHVHPRGWLEWNATFALDTLYYGEYMNYGPGGAVGQR 564

Query: 545 VTWPGYRVITSRVEASQFTVAQFIYGSSWLPSTGVAFLGGLNL 582
           VTWPGYRVITS VEAS+FTVAQFIYGSSWLPSTGVAFLGGLN+
Sbjct: 565 VTWPGYRVITSTVEASKFTVAQFIYGSSWLPSTGVAFLGGLNV 605

BLAST of CmoCh18G013460 vs. TrEMBL
Match: A0A067JU95_JATCU (Pectinesterase OS=Jatropha curcas GN=JCGZ_20123 PE=4 SV=1)

HSP 1 Score: 832.4 bits (2149), Expect = 3.4e-238
Identity = 420/573 (73.30%), Postives = 482/573 (84.12%), Query Frame = 1

Query: 16  RRGPLSVLSLLSLILILP-AVSPTSLLGLRTSSD----PKLPRKPTRAISKACARSRLPA 75
           R+  + +LSLLSL LI+  AVS T L+GLRT +     P L RKPT+AISKAC ++R P 
Sbjct: 34  RKKKIILLSLLSLALIIASAVSATLLIGLRTRASGQPGPALDRKPTKAISKACRKTRFPT 93

Query: 76  LCVDSLLDFPGSLSANERDLVHISFNLTLHHLNEALYSSSHLSSLHMDLPLRSAYDACLE 135
           LCV+SLLDFPGS +A+E+DLVHISFN+TL H ++ALY SS +S L MD  +RSAY+ CLE
Sbjct: 94  LCVNSLLDFPGSTTASEKDLVHISFNMTLQHFSKALYLSSAISYLQMDTRVRSAYEDCLE 153

Query: 136 LLDDSVHALTRSFHSLAPP--SSDSRPTPLASSSDDVITWLSAALTNQDTCTDGFSQLSG 195
           LLDDSV AL+RS  ++APP   +  +      S++DV+ WLSAALTNQDTCT+GF+ L+G
Sbjct: 154 LLDDSVDALSRSLSTVAPPIDGNTGQIQSAGGSTEDVVNWLSAALTNQDTCTEGFADLNG 213

Query: 196 NVKDQMTDKLHNLSELVSNCLALFSGSVRNDFSGVPIQNKRRLMNAAEKEDDSGNFPGWM 255
            VK+QMT KL +LSELVSNCLA+FS S  +DFSGVPIQN+RRLM A    +++   P W+
Sbjct: 214 AVKEQMTVKLKDLSELVSNCLAIFSASDDDDFSGVPIQNRRRLMVAEISGNNADELPSWL 273

Query: 256 SRRERRLLTLPVGALQADIVVSKDGNGTVKTIAEAIKKAPEFSTRKTIIYVMAGRYEEKN 315
            RRERRLL +PV A+QADI+VS+ GNGT KTI EAIKKAPE+S R+TIIYV AGRYEEK 
Sbjct: 274 GRRERRLLDMPVSAIQADIIVSQYGNGTYKTITEAIKKAPEYSNRRTIIYVTAGRYEEKI 333

Query: 316 LKVGRKKTNLMFVGDGKGKTVISGGKSIFDNVTTFHTASFAVSGAGIILRDMTFVNWAGP 375
           LKVGRKK NLMF+GDGKGKT+ISGGKSIFDN+TTFHTA+FA +GAG I RDMTF NWAGP
Sbjct: 334 LKVGRKKRNLMFIGDGKGKTIISGGKSIFDNITTFHTATFAATGAGFIARDMTFENWAGP 393

Query: 376 ARHQAVALRVSADHAVVYRCNVIGYQDTLYVHSNRQFFRECDIYGTVDFIFGNAAVVFQN 435
           ++HQAVALR+ ADHAVVYRCN+IGYQDTLYVHSNRQFFRECDIYGTVDFIFGNAAVVFQN
Sbjct: 394 SKHQAVALRIGADHAVVYRCNIIGYQDTLYVHSNRQFFRECDIYGTVDFIFGNAAVVFQN 453

Query: 436 CSIYARKAMELQKNTITAQNRKDPNQNTGISIHACRILATPDLASSSTATNPTYLGRPWK 495
           CSIYARK M  QKNTITAQNRKDPNQNTGISIHACRILAT DL  +S  + PTYLGRPWK
Sbjct: 454 CSIYARKPMAFQKNTITAQNRKDPNQNTGISIHACRILATSDL-EASKGSFPTYLGRPWK 513

Query: 496 LYSRVVYMQSYMGGHVHPRGWLEWNATFALDTLYYGEYMNYGPGGAVGQRVTWPGYRVIT 555
           LYSRVVYM SYMG H+HPRGWLEWN TFALDTLYYGEYMN GPGGA+GQRV WPGYRVIT
Sbjct: 514 LYSRVVYMLSYMGDHIHPRGWLEWNTTFALDTLYYGEYMNDGPGGALGQRVKWPGYRVIT 573

Query: 556 SRVEASQFTVAQFIYGSSWLPSTGVAFLGGLNL 582
           S VEAS+FTVA+FIYGSSWLPSTGVAFL GL++
Sbjct: 574 STVEASKFTVAEFIYGSSWLPSTGVAFLAGLSV 605

BLAST of CmoCh18G013460 vs. TrEMBL
Match: W9RDL0_9ROSA (Pectinesterase OS=Morus notabilis GN=L484_006819 PE=4 SV=1)

HSP 1 Score: 808.9 bits (2088), Expect = 4.0e-231
Identity = 413/574 (71.95%), Postives = 479/574 (83.45%), Query Frame = 1

Query: 20  LSVLSLLSLILILP-AVSPTSLLGLRT-----SSDPKLPRKPTRAISKACARSRLPALCV 79
           L VLS++S++LI   AVS   L+G+RT     S  P L  KPT+AIS+AC+++R P+LCV
Sbjct: 38  LVVLSVVSIVLITASAVSAVFLIGVRTRASGQSGHPGLAHKPTQAISRACSKTRFPSLCV 97

Query: 80  DSLLDFPGSLSANERDLVHISFNLTLHHLNEALYSSSHLSSLHMDLPLRSAYDACLELLD 139
           +SL+DFPG+L+A E DLVHISFN+TL  L++ALY+SS +S L MD   RSAYD CLELLD
Sbjct: 98  NSLVDFPGALTATEEDLVHISFNMTLQRLSKALYASSGISYLAMDPLSRSAYDGCLELLD 157

Query: 140 DSVHALTRSFHSLAPPSSDSRPTPLA-SSSDDVITWLSAALTNQDTCTDGFSQLSG-NVK 199
           DS+ AL+RS  S+AP  +     P++ +SS DV+TWLSAALTNQDTC +GF  ++G +VK
Sbjct: 158 DSIDALSRSLTSVAPSVAGGSAKPISGASSQDVVTWLSAALTNQDTCGEGFDGVAGGSVK 217

Query: 200 DQMTDKLHNLSELVSNCLALFSGSVRNDFSGVPIQNKRRLMNAAEKEDDSGNFPGWMSRR 259
             M + L +LSEL+SNCL++FSGSV +DFSGVPIQN+RRLM   +++D SG+FP W+ R+
Sbjct: 218 SHMAESLRDLSELLSNCLSIFSGSVTDDFSGVPIQNRRRLM---QEDDISGDFPRWLGRK 277

Query: 260 ERRLLTLPVGALQADIVVSKDGNGT----VKTIAEAIKKAPEFSTRKTIIYVMAGRYEEK 319
           ERRLL LPV A+QADIVVSKDGNG+     KTIAEAIKK PE STR+ +IYV AGRYEE 
Sbjct: 278 ERRLLALPVSAIQADIVVSKDGNGSNGREYKTIAEAIKKVPEHSTRRIVIYVKAGRYEEN 337

Query: 320 NLKVGRKKTNLMFVGDGKGKTVISGGKSIFDNVTTFHTASFAVSGAGIILRDMTFVNWAG 379
           NLKVGRKKTN+MF+GDG GKTVISGGKS+ D +TTFHTASFA +GAG I RD+TF NWAG
Sbjct: 338 NLKVGRKKTNVMFIGDGAGKTVISGGKSVSDKLTTFHTASFAATGAGFIARDITFTNWAG 397

Query: 380 PARHQAVALRVSADHAVVYRCNVIGYQDTLYVHSNRQFFRECDIYGTVDFIFGNAAVVFQ 439
           P RHQAVALR+ ADHAVVYRC  IGYQDTLYVHSNRQFFREC+IYGTVDFIFGNAAVVFQ
Sbjct: 398 PGRHQAVALRIGADHAVVYRCQAIGYQDTLYVHSNRQFFRECEIYGTVDFIFGNAAVVFQ 457

Query: 440 NCSIYARKAMELQKNTITAQNRKDPNQNTGISIHACRILATPDLASSSTATNPTYLGRPW 499
           NCSIYARK M  QKNTITAQNRKDPNQNTGISIHACRILAT DL  +S  + PTYLGRPW
Sbjct: 458 NCSIYARKPMPQQKNTITAQNRKDPNQNTGISIHACRILATSDL-QASKGSFPTYLGRPW 517

Query: 500 KLYSRVVYMQSYMGGHVHPRGWLEWNATFALDTLYYGEYMNYGPGGAVGQRVTWPGYRVI 559
           KLYSRVVY+ SYMG H+HPRGWLEWNATFALD+LYYGEYMN GPGGAVGQRV WPGYRVI
Sbjct: 518 KLYSRVVYLMSYMGDHIHPRGWLEWNATFALDSLYYGEYMNSGPGGAVGQRVKWPGYRVI 577

Query: 560 TSRVEASQFTVAQFIYGSSWLPSTGVAFLGGLNL 582
           TS VEAS+FTVAQFIYGSSWLPSTGVAFL GL++
Sbjct: 578 TSTVEASKFTVAQFIYGSSWLPSTGVAFLAGLSV 607

BLAST of CmoCh18G013460 vs. TrEMBL
Match: B9IDR8_POPTR (Pectinesterase OS=Populus trichocarpa GN=POPTR_0015s01610g PE=4 SV=2)

HSP 1 Score: 805.8 bits (2080), Expect = 3.4e-230
Identity = 407/579 (70.29%), Postives = 475/579 (82.04%), Query Frame = 1

Query: 11  PAANP-RRGPLSVLSLLSLILILP-AVSPTSLLGLRTSS----DPK--LPRKPTRAISKA 70
           P  +P R+  +  L++ S+ LI   AVS   LLG+RT +    DP     RKPT+AISK 
Sbjct: 31  PRVSPTRKKRIIFLAIFSIALIAASAVSAVLLLGIRTKASGQPDPSSLTHRKPTQAISKT 90

Query: 71  CARSRLPALCVDSLLDFPGSLSANERDLVHISFNLTLHHLNEALYSSSHLSSLHMDLPLR 130
           C+++R P LCV SLLDFPGS+SA+E DLVHISFN+TL H ++ALY SS +S ++M+  +R
Sbjct: 91  CSKTRFPNLCVSSLLDFPGSVSASESDLVHISFNMTLQHFSKALYLSSAISYVNMETRVR 150

Query: 131 SAYDACLELLDDSVHALTRSFHSLAPPSSDSRPTPLASSSDDVITWLSAALTNQDTCTDG 190
           SA+D CLELLDDS+ AL+RS  ++AP            S  DV+TWLSAALTNQDTC++G
Sbjct: 151 SAFDDCLELLDDSIDALSRSLSTVAPSHGGGTG---GGSPADVVTWLSAALTNQDTCSEG 210

Query: 191 FSQLSGNVKDQMTDKLHNLSELVSNCLALFSGSVRNDFSGVPIQNKRRLMNAAEKEDDSG 250
           F  ++G VK+QMT +L +L+ELVSNCLA+FS +  +DFSGVP+QNKRRL+   E      
Sbjct: 211 FDGVNGAVKNQMTGRLKDLTELVSNCLAIFSSANGDDFSGVPVQNKRRLLTENEDISYEE 270

Query: 251 NFPGWMSRRERRLLTLPVGALQADIVVSKDGNGTVKTIAEAIKKAPEFSTRKTIIYVMAG 310
           NFP W+ RR+R+LL +PV A+ ADI+VS DGNGT KTI+EAIKKAPE+STR+T+IYV AG
Sbjct: 271 NFPRWLGRRDRKLLDVPVPAIHADIIVSGDGNGTCKTISEAIKKAPEYSTRRTVIYVRAG 330

Query: 311 RYEEKNLKVGRKKTNLMFVGDGKGKTVISGGKSIFDNVTTFHTASFAVSGAGIILRDMTF 370
           RYEE NLKVGRKK NLMF+GDGKGKT+ISGGKS+F+N+TTFHTASFA +GAG I RDMTF
Sbjct: 331 RYEENNLKVGRKKWNLMFIGDGKGKTIISGGKSVFNNLTTFHTASFAATGAGFIARDMTF 390

Query: 371 VNWAGPARHQAVALRVSADHAVVYRCNVIGYQDTLYVHSNRQFFRECDIYGTVDFIFGNA 430
            NWAGPA+HQAVALRV ADHAVVYRCN+IGYQDTLYVHSNRQFFRECDIYGTVDFIFGNA
Sbjct: 391 ENWAGPAKHQAVALRVGADHAVVYRCNIIGYQDTLYVHSNRQFFRECDIYGTVDFIFGNA 450

Query: 431 AVVFQNCSIYARKAMELQKNTITAQNRKDPNQNTGISIHACRILATPDLASSSTATNPTY 490
           AVVFQNCSIYARK M  QKNTITAQNRKDPNQNTGISIHACRILAT DL +    + PT+
Sbjct: 451 AVVFQNCSIYARKPMAFQKNTITAQNRKDPNQNTGISIHACRILATSDL-TPLKGSFPTF 510

Query: 491 LGRPWKLYSRVVYMQSYMGGHVHPRGWLEWNATFALDTLYYGEYMNYGPGGAVGQRVTWP 550
           LGRPWKLYSR VYM SY+G HVHPRGWLEWN TFALDTLYYGEYMNYGPGGAVGQRV WP
Sbjct: 511 LGRPWKLYSRTVYMLSYIGDHVHPRGWLEWNTTFALDTLYYGEYMNYGPGGAVGQRVKWP 570

Query: 551 GYRVITSRVEASQFTVAQFIYGSSWLPSTGVAFLGGLNL 582
           GYRV+TS +EAS+FTVAQFIYGSSWLPSTGV+FL GL+L
Sbjct: 571 GYRVVTSTIEASKFTVAQFIYGSSWLPSTGVSFLAGLSL 605

BLAST of CmoCh18G013460 vs. TrEMBL
Match: B9I5B4_POPTR (Pectinesterase OS=Populus trichocarpa GN=POPTR_0012s02170g PE=4 SV=2)

HSP 1 Score: 797.3 bits (2058), Expect = 1.2e-227
Identity = 404/580 (69.66%), Postives = 472/580 (81.38%), Query Frame = 1

Query: 9   THPAANPRRGPLSVLSLLSLILILP-AVSPTSLLGLRTS----SDPK--LPRKPTRAISK 68
           T   +  R+  +  L++ S++LI   A S   LLG+R      SDP     RKPT+AISK
Sbjct: 30  TSQVSPTRKKKIIFLTIFSVVLIAASAASAAILLGVRDKASGQSDPNSLTHRKPTQAISK 89

Query: 69  ACARSRLPALCVDSLLDFPGSLSANERDLVHISFNLTLHHLNEALYSSSHLSSLHMDLPL 128
           AC+++R PALCV SLLDFPGS++A+E DLVHISFN+TL HL++ALY SS +S ++M+   
Sbjct: 90  ACSKTRFPALCVSSLLDFPGSMTASESDLVHISFNMTLLHLDKALYLSSGISYVNMETHE 149

Query: 129 RSAYDACLELLDDSVHALTRSFHSLAPPSSDSRPTPLASSSDDVITWLSAALTNQDTCTD 188
           RSA+D CLELL+DS+ ALTRS  +++P S          S +DV+TWLSAALTNQDTC++
Sbjct: 150 RSAFDDCLELLEDSIDALTRSLSTVSPSSGGG------GSPEDVVTWLSAALTNQDTCSE 209

Query: 189 GFSQLSGNVKDQMTDKLHNLSELVSNCLALFSGSVRNDFSGVPIQNKRRLMNAAEKEDDS 248
           GF  ++G VKDQMT+KL +L+ELVSNCLA+FS +   DFSGVPIQNKRRLM       + 
Sbjct: 210 GFEGVNGTVKDQMTEKLKDLTELVSNCLAIFSATNGGDFSGVPIQNKRRLMTEDGDISEE 269

Query: 249 GNFPGWMSRRERRLLTLPVGALQADIVVSKDGNGTVKTIAEAIKKAPEFSTRKTIIYVMA 308
            NFP W+ RRERRLL LPV A+QADI+VS DG+GT KTI+EAIKKAPE S R+TIIYV A
Sbjct: 270 DNFPSWLGRRERRLLGLPVSAIQADIIVSGDGSGTFKTISEAIKKAPEHSNRRTIIYVRA 329

Query: 309 GRYEEKNLKVGRKKTNLMFVGDGKGKTVISGGKSIFDNVTTFHTASFAVSGAGIILRDMT 368
           GRY E NLKVGRKK NLMF+GDG GKT+I+G +S+F+++TTFHTASFA +GAG I RDMT
Sbjct: 330 GRYVEDNLKVGRKKWNLMFIGDGMGKTIITGSRSVFNHITTFHTASFAATGAGFIARDMT 389

Query: 369 FVNWAGPARHQAVALRVSADHAVVYRCNVIGYQDTLYVHSNRQFFRECDIYGTVDFIFGN 428
           F NWAGPA+HQAVALRV ADHAVVYRC++IGYQDTLYVHSNRQF+RECDIYGTVDFIFGN
Sbjct: 390 FENWAGPAKHQAVALRVGADHAVVYRCSIIGYQDTLYVHSNRQFYRECDIYGTVDFIFGN 449

Query: 429 AAVVFQNCSIYARKAMELQKNTITAQNRKDPNQNTGISIHACRILATPDLASSSTATNPT 488
           AAVV QNCSIYARK M  QKNTITAQNRKDPNQNTGISIHAC+ILA  DLA S   T PT
Sbjct: 450 AAVVLQNCSIYARKPMASQKNTITAQNRKDPNQNTGISIHACKILAASDLAPSK-GTFPT 509

Query: 489 YLGRPWKLYSRVVYMQSYMGGHVHPRGWLEWNATFALDTLYYGEYMNYGPGGAVGQRVTW 548
           YLGRPWKLYSR VYM S+MG H+HPRGWLEW+A+FALDTLYYGEYMNYGPG AVGQRV W
Sbjct: 510 YLGRPWKLYSRTVYMLSFMGDHIHPRGWLEWDASFALDTLYYGEYMNYGPGAAVGQRVKW 569

Query: 549 PGYRVITSRVEASQFTVAQFIYGSSWLPSTGVAFLGGLNL 582
           PGYRVITS VEA++FTVAQFIYGSSWLPSTGVAFL GL++
Sbjct: 570 PGYRVITSTVEANKFTVAQFIYGSSWLPSTGVAFLAGLSV 602

BLAST of CmoCh18G013460 vs. TAIR10
Match: AT3G49220.1 (AT3G49220.1 Plant invertase/pectin methylesterase inhibitor superfamily)

HSP 1 Score: 753.8 bits (1945), Expect = 7.7e-218
Identity = 386/564 (68.44%), Postives = 451/564 (79.96%), Query Frame = 1

Query: 21  SVLSLLSLILILPAVSPTSLLGLRTSSDPKLPRKPTRAISKACARSRLPALCVDSLLDFP 80
           S++  +SLIL     +          S P L RKP++AISKAC  +R P LCVDSL+DFP
Sbjct: 49  SIVLAISLILAAAIFAGVRSRLKLNQSVPGLARKPSQAISKACELTRFPELCVDSLMDFP 108

Query: 81  GSLSANE-RDLVHISFNLTLHHLNEALYSSSHLSSLHMDLPLRSAYDACLELLDDSVHAL 140
           GSL+A+  +DL+H++ N+TLHH + ALYSS+ LS + M    RSAYD+C+ELLDDSV AL
Sbjct: 109 GSLAASSSKDLIHVTVNMTLHHFSHALYSSASLSFVDMPPRARSAYDSCVELLDDSVDAL 168

Query: 141 TRSFHSLAPPSSDSRPTPLASSSDDVITWLSAALTNQDTCTDGFSQLS-GNVKDQMTDKL 200
           +R+  S+   SS ++P        DV TWLSAALTN DTCT+GF  +  G VKD MT  L
Sbjct: 169 SRALSSVV--SSSAKP-------QDVTTWLSAALTNHDTCTEGFDGVDDGGVKDHMTAAL 228

Query: 201 HNLSELVSNCLALFSGSVR-NDFSGVPIQNKRRLMNAAEKEDDSGNFPGWMSRRERRLLT 260
            NLSELVSNCLA+FS S   +DF+GVPIQN RRL+   E+E+    FP WM  +ER +L 
Sbjct: 229 QNLSELVSNCLAIFSASHDGDDFAGVPIQN-RRLLGVEEREE---KFPRWMRPKEREILE 288

Query: 261 LPVGALQADIVVSKDGNGTVKTIAEAIKKAPEFSTRKTIIYVMAGRYEEKNLKVGRKKTN 320
           +PV  +QADI+VSKDGNGT KTI+EAIKKAP+ STR+ IIYV AGRYEE NLKVGRKK N
Sbjct: 289 MPVSQIQADIIVSKDGNGTCKTISEAIKKAPQNSTRRIIIYVKAGRYEENNLKVGRKKIN 348

Query: 321 LMFVGDGKGKTVISGGKSIFDNVTTFHTASFAVSGAGIILRDMTFVNWAGPARHQAVALR 380
           LMFVGDGKGKTVISGGKSIFDN+TTFHTASFA +GAG I RD+TF NWAGPA+HQAVALR
Sbjct: 349 LMFVGDGKGKTVISGGKSIFDNITTFHTASFAATGAGFIARDITFENWAGPAKHQAVALR 408

Query: 381 VSADHAVVYRCNVIGYQDTLYVHSNRQFFRECDIYGTVDFIFGNAAVVFQNCSIYARKAM 440
           + ADHAV+YRCN+IGYQDTLYVHSNRQFFRECDIYGTVDFIFGNAAVV QNCSIYARK M
Sbjct: 409 IGADHAVIYRCNIIGYQDTLYVHSNRQFFRECDIYGTVDFIFGNAAVVLQNCSIYARKPM 468

Query: 441 ELQKNTITAQNRKDPNQNTGISIHACRILATPDLASSSTATNPTYLGRPWKLYSRVVYMQ 500
           + QKNTITAQNRKDPNQNTGISIHA R+LA  DL +++ +T  TYLGRPWKL+SR VYM 
Sbjct: 469 DFQKNTITAQNRKDPNQNTGISIHASRVLAASDLQATNGSTQ-TYLGRPWKLFSRTVYMM 528

Query: 501 SYMGGHVHPRGWLEWNATFALDTLYYGEYMNYGPGGAVGQRVTWPGYRVITSRVEASQFT 560
           SY+GGHVH RGWLEWN TFALDTLYYGEY+N GPG  +GQRV+WPGYRVI S  EA++FT
Sbjct: 529 SYIGGHVHTRGWLEWNTTFALDTLYYGEYLNSGPGSGLGQRVSWPGYRVINSTAEANRFT 588

Query: 561 VAQFIYGSSWLPSTGVAFLGGLNL 582
           VA+FIYGSSWLPSTGV+FL GL++
Sbjct: 589 VAEFIYGSSWLPSTGVSFLAGLSI 598

BLAST of CmoCh18G013460 vs. TAIR10
Match: AT5G53370.1 (AT5G53370.1 pectin methylesterase PCR fragment F)

HSP 1 Score: 729.9 bits (1883), Expect = 1.2e-210
Identity = 378/599 (63.11%), Postives = 455/599 (75.96%), Query Frame = 1

Query: 1   MGCRRLNETHPAANPRRGPLSVLSLLS------LILILPAVSPTSLL------GLRT--- 60
           MG  RL  + P+   ++ P + L  L       LIL   AV    ++      G+R    
Sbjct: 1   MGYDRLGPSGPSNPNQKDPATSLPELQKKTKTKLILFTLAVLVVGVVCFGIFAGIRAVDS 60

Query: 61  -SSDPKLPRKPTRAISKACARSRLPALCVDSLLDFPGSLSANERDLVHISFNLTLHHLNE 120
             ++PKL RKPT+AIS+ C++S  P LC+D+LLDFPGSL+A+E +L+HISFN TL   ++
Sbjct: 61  GKTEPKLTRKPTQAISRTCSKSLYPNLCIDTLLDFPGSLTADENELIHISFNATLQKFSK 120

Query: 121 ALYSSSHLSSLHMDLPLRSAYDACLELLDDSVHALTRSFHSLAPPSSDSRPTPLASSSDD 180
           ALY+SS ++   M   +RSAYD+CLELLDDSV ALTR+  S+   S D        S  D
Sbjct: 121 ALYTSSTITYTQMPPRVRSAYDSCLELLDDSVDALTRALSSVVVVSGDE-------SHSD 180

Query: 181 VITWLSAALTNQDTCTDGFSQLSGN---VKDQMTDKLHNLSELVSNCLALFSGSVRNDFS 240
           V+TWLS+A+TN DTCTDGF ++ G    VKDQ+   + +LSE+VSNCLA+F+G V+ D S
Sbjct: 181 VMTWLSSAMTNHDTCTDGFDEIEGQGGEVKDQVIGAVKDLSEMVSNCLAIFAGKVK-DLS 240

Query: 241 GVPIQNKRRLMNAAEKEDDSGNFPGWMSRRERRLLTLPVGALQADIVVSKDGNGTVKTIA 300
           GVP+ N R+L+   E E+     P W+ R +R LL  P  A+QADI VSKDG+GT KTIA
Sbjct: 241 GVPVVNNRKLLGTEETEE----LPNWLKREDRELLGTPTSAIQADITVSKDGSGTFKTIA 300

Query: 301 EAIKKAPEFSTRKTIIYVMAGRYEEKNLKVGRKKTNLMFVGDGKGKTVISGGKSIFDNVT 360
           EAIKKAPE S+R+ +IYV AGRYEE+NLKVGRKKTNLMF+GDGKGKTVI+GGKSI D++T
Sbjct: 301 EAIKKAPEHSSRRFVIYVKAGRYEEENLKVGRKKTNLMFIGDGKGKTVITGGKSIADDLT 360

Query: 361 TFHTASFAVSGAGIILRDMTFVNWAGPARHQAVALRVSADHAVVYRCNVIGYQDTLYVHS 420
           TFHTA+FA +GAG I+RDMTF N+AGPA+HQAVALRV  DHAVVYRCN+IGYQD LYVHS
Sbjct: 361 TFHTATFAATGAGFIVRDMTFENYAGPAKHQAVALRVGGDHAVVYRCNIIGYQDALYVHS 420

Query: 421 NRQFFRECDIYGTVDFIFGNAAVVFQNCSIYARKAMELQKNTITAQNRKDPNQNTGISIH 480
           NRQFFREC+IYGTVDFIFGNAAV+ Q+C+IYARK M  QK TITAQNRKDPNQNTGISIH
Sbjct: 421 NRQFFRECEIYGTVDFIFGNAAVILQSCNIYARKPMAQQKITITAQNRKDPNQNTGISIH 480

Query: 481 ACRILATPDLASSSTATNPTYLGRPWKLYSRVVYMQSYMGGHVHPRGWLEWNATFALDTL 540
           AC++LATPDL  +S  + PTYLGRPWKLYSRVVYM S MG H+ PRGWLEWN  FALD+L
Sbjct: 481 ACKLLATPDL-EASKGSYPTYLGRPWKLYSRVVYMMSDMGDHIDPRGWLEWNGPFALDSL 540

Query: 541 YYGEYMNYGPGGAVGQRVTWPGYRVITSRVEASQFTVAQFIYGSSWLPSTGVAFLGGLN 581
           YYGEYMN G G  +GQRV WPGY VITS VEAS+FTVAQFI GSSWLPSTGV+F  GL+
Sbjct: 541 YYGEYMNKGLGSGIGQRVKWPGYHVITSTVEASKFTVAQFISGSSWLPSTGVSFFSGLS 586

BLAST of CmoCh18G013460 vs. TAIR10
Match: AT3G43270.1 (AT3G43270.1 Plant invertase/pectin methylesterase inhibitor superfamily)

HSP 1 Score: 411.8 bits (1057), Expect = 7.2e-115
Identity = 216/452 (47.79%), Postives = 281/452 (62.17%), Query Frame = 1

Query: 128 CLELLDDSVHALTRSFHSLAPPSSDSRPTPLASSSDDVITWLSAALTNQDTCTDGFSQLS 187
           C++LLD +   L+    +   P+     T    S  D+ TW+SAAL+NQDTC DGF   +
Sbjct: 84  CVDLLDSAAEELSWIISASQSPNGKDNSTGDVGS--DLRTWISAALSNQDTCLDGFEGTN 143

Query: 188 GNVKDQMTDKLHNLSELVSNCLALFSGSVRNDFSGVPIQNKRRLMNAAEKEDDSGNFPGW 247
           G +K  +   L  +   V N L +            P     +     +       FP W
Sbjct: 144 GIIKKIVAGGLSKVGTTVRNLLTMVHSP-----PSKPKPKPIKAQTMTKAHSGFSKFPSW 203

Query: 248 MSRRERRLLTLPVGALQADIVVSKDGNGTVKTIAEAIKKAPEFSTRKTIIYVMAGRYEEK 307
           +   +R+LL        AD VV+ DG G   TI++A+  AP++ST++ +I+V  G Y E 
Sbjct: 204 VKPGDRKLLQTD-NITVADAVVAADGTGNFTTISDAVLAAPDYSTKRYVIHVKRGVYVE- 263

Query: 308 NLKVGRKKTNLMFVGDGKGKTVISGGKSIFDNVTTFHTASFAVSGAGIILRDMTFVNWAG 367
           N+++ +KK N+M VGDG   TVI+G +S  D  TTF +A+FAVSG G I RD+TF N AG
Sbjct: 264 NVEIKKKKWNIMMVGDGIDATVITGNRSFIDGWTTFRSATFAVSGRGFIARDITFQNTAG 323

Query: 368 PARHQAVALRVSADHAVVYRCNVIGYQDTLYVHSNRQFFRECDIYGTVDFIFGNAAVVFQ 427
           P +HQAVA+R   D  V YRC + GYQDTLY HS RQFFREC I GTVDFIFG+A  VFQ
Sbjct: 324 PEKHQAVAIRSDTDLGVFYRCAMRGYQDTLYAHSMRQFFRECIITGTVDFIFGDATAVFQ 383

Query: 428 NCSIYARKAMELQKNTITAQNRKDPNQNTGISIHACRILATPDLASSSTATNPTYLGRPW 487
           +C I A++ +  QKN+ITAQ RKDPN+ TG +I    I A  DL  +   T  TYLGRPW
Sbjct: 384 SCQIKAKQGLPNQKNSITAQGRKDPNEPTGFTIQFSNIAADTDLLLNLN-TTATYLGRPW 443

Query: 488 KLYSRVVYMQSYMGGHVHPRGWLEWNATFALDTLYYGEYMNYGPGGAVGQRVTWPGYRVI 547
           KLYSR V+MQ+YM   ++P GWLEWN  FALDTLYYGEYMN GPG ++ +RV WPGY V+
Sbjct: 444 KLYSRTVFMQNYMSDAINPVGWLEWNGNFALDTLYYGEYMNSGPGASLDRRVKWPGYHVL 503

Query: 548 TSRVEASQFTVAQFIYGSSWLPSTGVAFLGGL 580
            +  EA+ FTV+Q I G+ WLPSTG+ F+ GL
Sbjct: 504 NTSAEANNFTVSQLIQGNLWLPSTGITFIAGL 525

BLAST of CmoCh18G013460 vs. TAIR10
Match: AT1G53830.1 (AT1G53830.1 pectin methylesterase 2)

HSP 1 Score: 408.7 bits (1049), Expect = 6.1e-114
Identity = 226/532 (42.48%), Postives = 308/532 (57.89%), Query Frame = 1

Query: 59  ISKACARSRLPALCVDSLLDFPGSLSANERDLVHISFNLTLHHLNEALYSSSHLSSLHMD 118
           +   C+ +  P LC  ++    G    ++++++  S NLT   +    ++   L +    
Sbjct: 62  LKSVCSSTLYPELCFSAVAATGGKELTSQKEVIEASLNLTTKAVKHNYFAVKKLIAKRKG 121

Query: 119 LPLR--SAYDACLELLD---DSVHALTRSFHSLAPPSSDSRPTPLASSSDDVITWLSAAL 178
           L  R  +A   CLE +D   D +H      H      S      L   +DD+ T +S+A+
Sbjct: 122 LTPREVTALHDCLETIDETLDELHVAVEDLHQYPKQKS------LRKHADDLKTLISSAI 181

Query: 179 TNQDTCTDGFS--QLSGNVKDQMTDKLHNLSELVSNCLALFSGSVRNDFSGVPIQNK--- 238
           TNQ TC DGFS       V+  +     ++  + SN LA+       D +   +++K   
Sbjct: 182 TNQGTCLDGFSYDDADRKVRKALLKGQVHVEHMCSNALAMIKNMTETDIANFELRDKSST 241

Query: 239 -----RRLMNAAEKEDDSGNFPGWMSRRERRLLTLPVGALQADIVVSKDGNGTVKTIAEA 298
                 R +     + DS  +P W+S  +RRLL      ++AD  V+ DG+G   T+A A
Sbjct: 242 FTNNNNRKLKEVTGDLDSDGWPKWLSVGDRRLLQ--GSTIKADATVADDGSGDFTTVAAA 301

Query: 299 IKKAPEFSTRKTIIYVMAGRYEEKNLKVGRKKTNLMFVGDGKGKTVISGGKSIFDNVTTF 358
           +  APE S ++ +I++ AG Y E N++V +KKTN+MF+GDG+GKT+I+G +++ D  TTF
Sbjct: 302 VAAAPEKSNKRFVIHIKAGVYRE-NVEVTKKKTNIMFLGDGRGKTIITGSRNVVDGSTTF 361

Query: 359 HTASFAVSGAGIILRDMTFVNWAGPARHQAVALRVSADHAVVYRCNVIGYQDTLYVHSNR 418
           H+A+ A  G   + RD+TF N AGP++HQAVALRV +D +  Y+C++  YQDTLYVHSNR
Sbjct: 362 HSATVAAVGERFLARDITFQNTAGPSKHQAVALRVGSDFSAFYQCDMFAYQDTLYVHSNR 421

Query: 419 QFFRECDIYGTVDFIFGNAAVVFQNCSIYARKAMELQKNTITAQNRKDPNQNTGISIHAC 478
           QFF +C I GTVDFIFGNAA V Q+C I AR+    QKN +TAQ R DPNQNTGI I  C
Sbjct: 422 QFFVKCHITGTVDFIFGNAAAVLQDCDINARRPNSGQKNMVTAQGRSDPNQNTGIVIQNC 481

Query: 479 RILATPDLASSSTATNPTYLGRPWKLYSRVVYMQSYMGGHVHPRGWLEWNATFALDTLYY 538
           RI  T DL +    T PTYLGRPWK YSR V MQS +   + P GW EW+ +FALDTL Y
Sbjct: 482 RIGGTSDLLAVK-GTFPTYLGRPWKEYSRTVIMQSDISDVIRPEGWHEWSGSFALDTLTY 541

Query: 539 GEYMNYGPGGAVGQRVTWPGYRVITSRVEASQFTVAQFIYGSSWLPSTGVAF 576
            EY+N G G     RV W GY+VITS  EA  FT  QFI G  WL STG  F
Sbjct: 542 REYLNRGGGAGTANRVKWKGYKVITSDTEAQPFTAGQFIGGGGWLASTGFPF 583

BLAST of CmoCh18G013460 vs. TAIR10
Match: AT3G14310.1 (AT3G14310.1 pectin methylesterase 3)

HSP 1 Score: 404.4 bits (1038), Expect = 1.1e-112
Identity = 237/583 (40.65%), Postives = 331/583 (56.78%), Query Frame = 1

Query: 20  LSVLSLLSLILILPAVSPTSLLGLRTSSDPKLPRKPTRAISKACARSRLPALCVDSLLDF 79
           L +LS    +L + AV+  S    + +    L       +  +C+ +R P LC+ +++  
Sbjct: 20  LVLLSAAVALLFVAAVAGISAGASKANEKRTLSPSSHAVLRSSCSSTRYPELCISAVVTA 79

Query: 80  PGSLSANERDLVHISFNLTLHHLNEALYSSSHLSSLHMDLPLR--SAYDACLELLD---D 139
            G    +++D++  S NLT+  +    ++   L      L  R  +A   CLE +D   D
Sbjct: 80  GGVELTSQKDVIEASVNLTITAVEHNYFTVKKLIKKRKGLTPREKTALHDCLETIDETLD 139

Query: 140 SVHALTRSFHSLAPPSSDSRPTPLASSSDDVITWLSAALTNQDTCTDGFSQLSGN--VKD 199
            +H      H L P     R       + D+ T +S+A+TNQ+TC DGFS    +  V+ 
Sbjct: 140 ELHETVEDLH-LYPTKKTLR-----EHAGDLKTLISSAITNQETCLDGFSHDDADKQVRK 199

Query: 200 QMTDKLHNLSELVSNCLALFSGSVRNDFSGVPIQNKRRLMNAAEKED------------- 259
            +     ++  + SN LA+       D +    + K    N   KE+             
Sbjct: 200 ALLKGQIHVEHMCSNALAMIKNMTDTDIANFEQKAKITSNNRKLKEENQETTVAVDIAGA 259

Query: 260 ---DSGNFPGWMSRRERRLLTLPVGALQADIVVSKDGNGTVKTIAEAIKKAPEFSTRKTI 319
              DS  +P W+S  +RRLL      ++AD  V+ DG+GT KT+A A+  APE S ++ +
Sbjct: 260 GELDSEGWPTWLSAGDRRLLQ--GSGVKADATVAADGSGTFKTVAAAVAAAPENSNKRYV 319

Query: 320 IYVMAGRYEEKNLKVGRKKTNLMFVGDGKGKTVISGGKSIFDNVTTFHTASFAVSGAGII 379
           I++ AG Y E N++V +KK N+MF+GDG+ +T+I+G +++ D  TTFH+A+ A  G   +
Sbjct: 320 IHIKAGVYRE-NVEVAKKKKNIMFMGDGRTRTIITGSRNVVDGSTTFHSATVAAVGERFL 379

Query: 380 LRDMTFVNWAGPARHQAVALRVSADHAVVYRCNVIGYQDTLYVHSNRQFFRECDIYGTVD 439
            RD+TF N AGP++HQAVALRV +D +  Y C+++ YQDTLYVHSNRQFF +C I GTVD
Sbjct: 380 ARDITFQNTAGPSKHQAVALRVGSDFSAFYNCDMLAYQDTLYVHSNRQFFVKCLIAGTVD 439

Query: 440 FIFGNAAVVFQNCSIYARKAMELQKNTITAQNRKDPNQNTGISIHACRILATPDLASSST 499
           FIFGNAAVV Q+C I+AR+    QKN +TAQ R DPNQNTGI I  CRI AT DL  S  
Sbjct: 440 FIFGNAAVVLQDCDIHARRPNSGQKNMVTAQGRTDPNQNTGIVIQKCRIGATSDL-QSVK 499

Query: 500 ATNPTYLGRPWKLYSRVVYMQSYMGGHVHPRGWLEWNATFALDTLYYGEYMNYGPGGAVG 559
            + PTYLGRPWK YS+ V MQS +   + P GW EW  TFAL+TL Y EY N G G    
Sbjct: 500 GSFPTYLGRPWKEYSQTVIMQSAISDVIRPEGWSEWTGTFALNTLTYREYSNTGAGAGTA 559

Query: 560 QRVTWPGYRVITSRVEASQFTVAQFIYGSSWLPSTGVAFLGGL 580
            RV W G++VIT+  EA ++T  QFI G  WL STG  F  GL
Sbjct: 560 NRVKWRGFKVITAAAEAQKYTAGQFIGGGGWLSSTGFPFSLGL 592

BLAST of CmoCh18G013460 vs. NCBI nr
Match: gi|449456965|ref|XP_004146219.1| (PREDICTED: probable pectinesterase/pectinesterase inhibitor 34 [Cucumis sativus])

HSP 1 Score: 941.0 bits (2431), Expect = 9.7e-271
Identity = 478/583 (81.99%), Postives = 521/583 (89.37%), Query Frame = 1

Query: 5   RLNETHPAANPRRGPLSVLSLLSLILILPA-VSPTSLLGLRTSS----DPKLPRKPTRAI 64
           +L+  HP  N R   L ++S LSL+LI+ A VS T L+GLRT+S    DP LPRKPT+AI
Sbjct: 25  QLSSHHPTPNRRTTRLLLISFLSLLLIVAAAVSATFLIGLRTASPARSDPNLPRKPTQAI 84

Query: 65  SKACARSRLPALCVDSLLDFPGSLSANERDLVHISFNLTLHHLNEALYSSSHLSSLHMDL 124
           SKAC+R+R P LCV+SLLDFPGSL+ANE+DLVHISFN+TL HLN+ALYSSS +SSL MDL
Sbjct: 85  SKACSRTRFPTLCVNSLLDFPGSLNANEQDLVHISFNVTLQHLNKALYSSSEISSLQMDL 144

Query: 125 PLRSAYDACLELLDDSVHALTRSFHSLAPPSSDSRPTPLASSSDDVITWLSAALTNQDTC 184
            +RSAYDACLELLDDS+ A++RS  S+AP SS S P  L SS +D+ITWLSAALTNQDTC
Sbjct: 145 RVRSAYDACLELLDDSIDAVSRSLQSVAPSSSSSTPQRLGSS-EDIITWLSAALTNQDTC 204

Query: 185 TDGFSQLSGNVKDQMTDKLHNLSELVSNCLALFSGSVRNDFSGVPIQNKRRLMNA-AEKE 244
           TDGFS+LSG VK+QM DKLHNLSELVSNCLALFSGS  +DF+GVPIQNKRRLM    E E
Sbjct: 205 TDGFSELSGTVKNQMADKLHNLSELVSNCLALFSGSETSDFAGVPIQNKRRLMEEEGENE 264

Query: 245 DDSGNFPGWMSRRERRLLTLPVGALQADIVVSKDGNGTVKTIAEAIKKAPEFSTRKTIIY 304
           D SG FP WM+RRERRLLTLPVG LQADIVVS++GNGTVKTIAEAIKKAP++S+R+TIIY
Sbjct: 265 DSSGKFPRWMNRRERRLLTLPVGVLQADIVVSQNGNGTVKTIAEAIKKAPQYSSRRTIIY 324

Query: 305 VMAGRYEEKNLKVGRKKTNLMFVGDGKGKTVISGGKSIFDNVTTFHTASFAVSGAGIILR 364
           VMAGRYEEKNLKVGRKKTNLMFVGDGKGKTVISG KSIFDNVTTFHTASFA +GAGIILR
Sbjct: 325 VMAGRYEEKNLKVGRKKTNLMFVGDGKGKTVISGSKSIFDNVTTFHTASFAATGAGIILR 384

Query: 365 DMTFVNWAGPARHQAVALRVSADHAVVYRCNVIGYQDTLYVHSNRQFFRECDIYGTVDFI 424
           DMTF NWAGP RHQAVALRV ADHAVVYRCN+IGYQDTLYVHSNRQF+RECDIYGTVDFI
Sbjct: 385 DMTFENWAGPGRHQAVALRVGADHAVVYRCNIIGYQDTLYVHSNRQFYRECDIYGTVDFI 444

Query: 425 FGNAAVVFQNCSIYARKAMELQKNTITAQNRKDPNQNTGISIHACRILATPDLASSSTAT 484
           FGNAAVVFQNCSIYARKAM LQKNTITAQNRKDPNQNTGISIHACRILAT DL SS+T +
Sbjct: 445 FGNAAVVFQNCSIYARKAMALQKNTITAQNRKDPNQNTGISIHACRILATSDLESSNT-S 504

Query: 485 NPTYLGRPWKLYSRVVYMQSYMGGHVHPRGWLEWNATFALDTLYYGEYMNYGPGGAVGQR 544
           NPTYLGRPWKLYSR V M SY+G HVHPRGWLEWNATFALDTLYYGEYMNYGPGGAVGQR
Sbjct: 505 NPTYLGRPWKLYSRTVVMLSYIGNHVHPRGWLEWNATFALDTLYYGEYMNYGPGGAVGQR 564

Query: 545 VTWPGYRVITSRVEASQFTVAQFIYGSSWLPSTGVAFLGGLNL 582
           VTWPGYRVITS VEAS+FTVAQFIYGSSWLPSTGVAFLGGLN+
Sbjct: 565 VTWPGYRVITSTVEASKFTVAQFIYGSSWLPSTGVAFLGGLNV 605

BLAST of CmoCh18G013460 vs. NCBI nr
Match: gi|802709095|ref|XP_012084535.1| (PREDICTED: probable pectinesterase/pectinesterase inhibitor 34 [Jatropha curcas])

HSP 1 Score: 832.4 bits (2149), Expect = 4.8e-238
Identity = 420/573 (73.30%), Postives = 482/573 (84.12%), Query Frame = 1

Query: 16  RRGPLSVLSLLSLILILP-AVSPTSLLGLRTSSD----PKLPRKPTRAISKACARSRLPA 75
           R+  + +LSLLSL LI+  AVS T L+GLRT +     P L RKPT+AISKAC ++R P 
Sbjct: 34  RKKKIILLSLLSLALIIASAVSATLLIGLRTRASGQPGPALDRKPTKAISKACRKTRFPT 93

Query: 76  LCVDSLLDFPGSLSANERDLVHISFNLTLHHLNEALYSSSHLSSLHMDLPLRSAYDACLE 135
           LCV+SLLDFPGS +A+E+DLVHISFN+TL H ++ALY SS +S L MD  +RSAY+ CLE
Sbjct: 94  LCVNSLLDFPGSTTASEKDLVHISFNMTLQHFSKALYLSSAISYLQMDTRVRSAYEDCLE 153

Query: 136 LLDDSVHALTRSFHSLAPP--SSDSRPTPLASSSDDVITWLSAALTNQDTCTDGFSQLSG 195
           LLDDSV AL+RS  ++APP   +  +      S++DV+ WLSAALTNQDTCT+GF+ L+G
Sbjct: 154 LLDDSVDALSRSLSTVAPPIDGNTGQIQSAGGSTEDVVNWLSAALTNQDTCTEGFADLNG 213

Query: 196 NVKDQMTDKLHNLSELVSNCLALFSGSVRNDFSGVPIQNKRRLMNAAEKEDDSGNFPGWM 255
            VK+QMT KL +LSELVSNCLA+FS S  +DFSGVPIQN+RRLM A    +++   P W+
Sbjct: 214 AVKEQMTVKLKDLSELVSNCLAIFSASDDDDFSGVPIQNRRRLMVAEISGNNADELPSWL 273

Query: 256 SRRERRLLTLPVGALQADIVVSKDGNGTVKTIAEAIKKAPEFSTRKTIIYVMAGRYEEKN 315
            RRERRLL +PV A+QADI+VS+ GNGT KTI EAIKKAPE+S R+TIIYV AGRYEEK 
Sbjct: 274 GRRERRLLDMPVSAIQADIIVSQYGNGTYKTITEAIKKAPEYSNRRTIIYVTAGRYEEKI 333

Query: 316 LKVGRKKTNLMFVGDGKGKTVISGGKSIFDNVTTFHTASFAVSGAGIILRDMTFVNWAGP 375
           LKVGRKK NLMF+GDGKGKT+ISGGKSIFDN+TTFHTA+FA +GAG I RDMTF NWAGP
Sbjct: 334 LKVGRKKRNLMFIGDGKGKTIISGGKSIFDNITTFHTATFAATGAGFIARDMTFENWAGP 393

Query: 376 ARHQAVALRVSADHAVVYRCNVIGYQDTLYVHSNRQFFRECDIYGTVDFIFGNAAVVFQN 435
           ++HQAVALR+ ADHAVVYRCN+IGYQDTLYVHSNRQFFRECDIYGTVDFIFGNAAVVFQN
Sbjct: 394 SKHQAVALRIGADHAVVYRCNIIGYQDTLYVHSNRQFFRECDIYGTVDFIFGNAAVVFQN 453

Query: 436 CSIYARKAMELQKNTITAQNRKDPNQNTGISIHACRILATPDLASSSTATNPTYLGRPWK 495
           CSIYARK M  QKNTITAQNRKDPNQNTGISIHACRILAT DL  +S  + PTYLGRPWK
Sbjct: 454 CSIYARKPMAFQKNTITAQNRKDPNQNTGISIHACRILATSDL-EASKGSFPTYLGRPWK 513

Query: 496 LYSRVVYMQSYMGGHVHPRGWLEWNATFALDTLYYGEYMNYGPGGAVGQRVTWPGYRVIT 555
           LYSRVVYM SYMG H+HPRGWLEWN TFALDTLYYGEYMN GPGGA+GQRV WPGYRVIT
Sbjct: 514 LYSRVVYMLSYMGDHIHPRGWLEWNTTFALDTLYYGEYMNDGPGGALGQRVKWPGYRVIT 573

Query: 556 SRVEASQFTVAQFIYGSSWLPSTGVAFLGGLNL 582
           S VEAS+FTVA+FIYGSSWLPSTGVAFL GL++
Sbjct: 574 STVEASKFTVAEFIYGSSWLPSTGVAFLAGLSV 605

BLAST of CmoCh18G013460 vs. NCBI nr
Match: gi|1009123309|ref|XP_015878472.1| (PREDICTED: probable pectinesterase/pectinesterase inhibitor 34 [Ziziphus jujuba])

HSP 1 Score: 814.7 bits (2103), Expect = 1.0e-232
Identity = 412/580 (71.03%), Postives = 481/580 (82.93%), Query Frame = 1

Query: 9   THPAANPRRGPLSVLSLLSLILILP-AVSPTSLLGLRTSSDPK----LPRKPTRAISKAC 68
           T P  N ++  +  LS+L++ LI   A+S    +G+RT +       L RKPT+AIS+AC
Sbjct: 29  TRPVRNRKK--IIFLSVLAIALIAASAISAVLFIGVRTKASGSPGSVLHRKPTQAISRAC 88

Query: 69  ARSRLPALCVDSLLDFPGSLSANERDLVHISFNLTLHHLNEALYSSSHLSSLHMDLPLRS 128
           +++R  +LCV+SLLDFPGSL+A+E+DLVHISFN+TL H+++ALY SS +S L MD  +RS
Sbjct: 89  SKTRYESLCVNSLLDFPGSLNASEQDLVHISFNMTLQHISKALYLSSGISYLQMDPHVRS 148

Query: 129 AYDACLELLDDSVHALTRSFHSLAPPSSDSRPTPLASSSDDVITWLSAALTNQDTCTDGF 188
           AYD C+ELL+DS+ AL+RS  S+ P    +       S  DV+TWLSAALTNQDTCT+GF
Sbjct: 149 AYDDCVELLEDSIEALSRSLTSVVPSDGATNRQLSGGSVQDVMTWLSAALTNQDTCTEGF 208

Query: 189 SQLSGNVKDQMTDKLHNLSELVSNCLALFSGSVRNDFSGVPIQNKRRLMNA---AEKEDD 248
           S LSGNVKDQM ++L +LSELVSNCLA+F+ S  +DFSGVPIQN+RRL+        ++ 
Sbjct: 209 SDLSGNVKDQMGERLKDLSELVSNCLAIFAASDSDDFSGVPIQNRRRLLEEDSDISPKNA 268

Query: 249 SGNFPGWMSRRERRLLTLPVGALQADIVVSKDGNGTVKTIAEAIKKAPEFSTRKTIIYVM 308
           +G+FP W+ R++R+LL LPV  +QADIVVSKDGNGTVKTIAEAIKKAP+ S R+TIIYV 
Sbjct: 269 AGDFPRWLGRKDRKLLALPVSEIQADIVVSKDGNGTVKTIAEAIKKAPQSSNRRTIIYVR 328

Query: 309 AGRYEEKNLKVGRKKTNLMFVGDGKGKTVISGGKSIFDNVTTFHTASFAVSGAGIILRDM 368
           AGRYEE NLKVGRKKTNLMF+GDGKGKT+ISGGKS+ D +TTFHTASFA +GAG I RDM
Sbjct: 329 AGRYEEDNLKVGRKKTNLMFIGDGKGKTIISGGKSVKDKLTTFHTASFAATGAGFIARDM 388

Query: 369 TFVNWAGPARHQAVALRVSADHAVVYRCNVIGYQDTLYVHSNRQFFRECDIYGTVDFIFG 428
           TF NWAGPA+HQAVALRV ADHAVVYRCNVIGYQDTLYVHSNRQFFRECD+YGTVDFIFG
Sbjct: 389 TFENWAGPAKHQAVALRVGADHAVVYRCNVIGYQDTLYVHSNRQFFRECDVYGTVDFIFG 448

Query: 429 NAAVVFQNCSIYARKAMELQKNTITAQNRKDPNQNTGISIHACRILATPDLASSSTATNP 488
           NAAVVFQNCS++ARK M +QKNTITAQNRKDPNQNTGISIHACRILAT DL +S  + + 
Sbjct: 449 NAAVVFQNCSMFARKPMAMQKNTITAQNRKDPNQNTGISIHACRILATSDLEASKGSFS- 508

Query: 489 TYLGRPWKLYSRVVYMQSYMGGHVHPRGWLEWNATFALDTLYYGEYMNYGPGGAVGQRVT 548
           TYLGRPWKLYSR VYM SYMG H+HPRGWLEWNA FALDTLYYGEYMN GPGGAVGQRV 
Sbjct: 509 TYLGRPWKLYSRTVYMLSYMGDHIHPRGWLEWNAAFALDTLYYGEYMNSGPGGAVGQRVK 568

Query: 549 WPGYRVITSRVEASQFTVAQFIYGSSWLPSTGVAFLGGLN 581
           WPGYRVITS VEAS+FTVAQFIYGSSWLPSTGVAFL GL+
Sbjct: 569 WPGYRVITSTVEASKFTVAQFIYGSSWLPSTGVAFLAGLS 605

BLAST of CmoCh18G013460 vs. NCBI nr
Match: gi|703114846|ref|XP_010100757.1| (putative pectinesterase/pectinesterase inhibitor 34 [Morus notabilis])

HSP 1 Score: 808.9 bits (2088), Expect = 5.7e-231
Identity = 413/574 (71.95%), Postives = 479/574 (83.45%), Query Frame = 1

Query: 20  LSVLSLLSLILILP-AVSPTSLLGLRT-----SSDPKLPRKPTRAISKACARSRLPALCV 79
           L VLS++S++LI   AVS   L+G+RT     S  P L  KPT+AIS+AC+++R P+LCV
Sbjct: 38  LVVLSVVSIVLITASAVSAVFLIGVRTRASGQSGHPGLAHKPTQAISRACSKTRFPSLCV 97

Query: 80  DSLLDFPGSLSANERDLVHISFNLTLHHLNEALYSSSHLSSLHMDLPLRSAYDACLELLD 139
           +SL+DFPG+L+A E DLVHISFN+TL  L++ALY+SS +S L MD   RSAYD CLELLD
Sbjct: 98  NSLVDFPGALTATEEDLVHISFNMTLQRLSKALYASSGISYLAMDPLSRSAYDGCLELLD 157

Query: 140 DSVHALTRSFHSLAPPSSDSRPTPLA-SSSDDVITWLSAALTNQDTCTDGFSQLSG-NVK 199
           DS+ AL+RS  S+AP  +     P++ +SS DV+TWLSAALTNQDTC +GF  ++G +VK
Sbjct: 158 DSIDALSRSLTSVAPSVAGGSAKPISGASSQDVVTWLSAALTNQDTCGEGFDGVAGGSVK 217

Query: 200 DQMTDKLHNLSELVSNCLALFSGSVRNDFSGVPIQNKRRLMNAAEKEDDSGNFPGWMSRR 259
             M + L +LSEL+SNCL++FSGSV +DFSGVPIQN+RRLM   +++D SG+FP W+ R+
Sbjct: 218 SHMAESLRDLSELLSNCLSIFSGSVTDDFSGVPIQNRRRLM---QEDDISGDFPRWLGRK 277

Query: 260 ERRLLTLPVGALQADIVVSKDGNGT----VKTIAEAIKKAPEFSTRKTIIYVMAGRYEEK 319
           ERRLL LPV A+QADIVVSKDGNG+     KTIAEAIKK PE STR+ +IYV AGRYEE 
Sbjct: 278 ERRLLALPVSAIQADIVVSKDGNGSNGREYKTIAEAIKKVPEHSTRRIVIYVKAGRYEEN 337

Query: 320 NLKVGRKKTNLMFVGDGKGKTVISGGKSIFDNVTTFHTASFAVSGAGIILRDMTFVNWAG 379
           NLKVGRKKTN+MF+GDG GKTVISGGKS+ D +TTFHTASFA +GAG I RD+TF NWAG
Sbjct: 338 NLKVGRKKTNVMFIGDGAGKTVISGGKSVSDKLTTFHTASFAATGAGFIARDITFTNWAG 397

Query: 380 PARHQAVALRVSADHAVVYRCNVIGYQDTLYVHSNRQFFRECDIYGTVDFIFGNAAVVFQ 439
           P RHQAVALR+ ADHAVVYRC  IGYQDTLYVHSNRQFFREC+IYGTVDFIFGNAAVVFQ
Sbjct: 398 PGRHQAVALRIGADHAVVYRCQAIGYQDTLYVHSNRQFFRECEIYGTVDFIFGNAAVVFQ 457

Query: 440 NCSIYARKAMELQKNTITAQNRKDPNQNTGISIHACRILATPDLASSSTATNPTYLGRPW 499
           NCSIYARK M  QKNTITAQNRKDPNQNTGISIHACRILAT DL  +S  + PTYLGRPW
Sbjct: 458 NCSIYARKPMPQQKNTITAQNRKDPNQNTGISIHACRILATSDL-QASKGSFPTYLGRPW 517

Query: 500 KLYSRVVYMQSYMGGHVHPRGWLEWNATFALDTLYYGEYMNYGPGGAVGQRVTWPGYRVI 559
           KLYSRVVY+ SYMG H+HPRGWLEWNATFALD+LYYGEYMN GPGGAVGQRV WPGYRVI
Sbjct: 518 KLYSRVVYLMSYMGDHIHPRGWLEWNATFALDSLYYGEYMNSGPGGAVGQRVKWPGYRVI 577

Query: 560 TSRVEASQFTVAQFIYGSSWLPSTGVAFLGGLNL 582
           TS VEAS+FTVAQFIYGSSWLPSTGVAFL GL++
Sbjct: 578 TSTVEASKFTVAQFIYGSSWLPSTGVAFLAGLSV 607

BLAST of CmoCh18G013460 vs. NCBI nr
Match: gi|566205409|ref|XP_002321999.2| (pectin methylesterase family protein [Populus trichocarpa])

HSP 1 Score: 805.8 bits (2080), Expect = 4.8e-230
Identity = 407/579 (70.29%), Postives = 475/579 (82.04%), Query Frame = 1

Query: 11  PAANP-RRGPLSVLSLLSLILILP-AVSPTSLLGLRTSS----DPK--LPRKPTRAISKA 70
           P  +P R+  +  L++ S+ LI   AVS   LLG+RT +    DP     RKPT+AISK 
Sbjct: 31  PRVSPTRKKRIIFLAIFSIALIAASAVSAVLLLGIRTKASGQPDPSSLTHRKPTQAISKT 90

Query: 71  CARSRLPALCVDSLLDFPGSLSANERDLVHISFNLTLHHLNEALYSSSHLSSLHMDLPLR 130
           C+++R P LCV SLLDFPGS+SA+E DLVHISFN+TL H ++ALY SS +S ++M+  +R
Sbjct: 91  CSKTRFPNLCVSSLLDFPGSVSASESDLVHISFNMTLQHFSKALYLSSAISYVNMETRVR 150

Query: 131 SAYDACLELLDDSVHALTRSFHSLAPPSSDSRPTPLASSSDDVITWLSAALTNQDTCTDG 190
           SA+D CLELLDDS+ AL+RS  ++AP            S  DV+TWLSAALTNQDTC++G
Sbjct: 151 SAFDDCLELLDDSIDALSRSLSTVAPSHGGGTG---GGSPADVVTWLSAALTNQDTCSEG 210

Query: 191 FSQLSGNVKDQMTDKLHNLSELVSNCLALFSGSVRNDFSGVPIQNKRRLMNAAEKEDDSG 250
           F  ++G VK+QMT +L +L+ELVSNCLA+FS +  +DFSGVP+QNKRRL+   E      
Sbjct: 211 FDGVNGAVKNQMTGRLKDLTELVSNCLAIFSSANGDDFSGVPVQNKRRLLTENEDISYEE 270

Query: 251 NFPGWMSRRERRLLTLPVGALQADIVVSKDGNGTVKTIAEAIKKAPEFSTRKTIIYVMAG 310
           NFP W+ RR+R+LL +PV A+ ADI+VS DGNGT KTI+EAIKKAPE+STR+T+IYV AG
Sbjct: 271 NFPRWLGRRDRKLLDVPVPAIHADIIVSGDGNGTCKTISEAIKKAPEYSTRRTVIYVRAG 330

Query: 311 RYEEKNLKVGRKKTNLMFVGDGKGKTVISGGKSIFDNVTTFHTASFAVSGAGIILRDMTF 370
           RYEE NLKVGRKK NLMF+GDGKGKT+ISGGKS+F+N+TTFHTASFA +GAG I RDMTF
Sbjct: 331 RYEENNLKVGRKKWNLMFIGDGKGKTIISGGKSVFNNLTTFHTASFAATGAGFIARDMTF 390

Query: 371 VNWAGPARHQAVALRVSADHAVVYRCNVIGYQDTLYVHSNRQFFRECDIYGTVDFIFGNA 430
            NWAGPA+HQAVALRV ADHAVVYRCN+IGYQDTLYVHSNRQFFRECDIYGTVDFIFGNA
Sbjct: 391 ENWAGPAKHQAVALRVGADHAVVYRCNIIGYQDTLYVHSNRQFFRECDIYGTVDFIFGNA 450

Query: 431 AVVFQNCSIYARKAMELQKNTITAQNRKDPNQNTGISIHACRILATPDLASSSTATNPTY 490
           AVVFQNCSIYARK M  QKNTITAQNRKDPNQNTGISIHACRILAT DL +    + PT+
Sbjct: 451 AVVFQNCSIYARKPMAFQKNTITAQNRKDPNQNTGISIHACRILATSDL-TPLKGSFPTF 510

Query: 491 LGRPWKLYSRVVYMQSYMGGHVHPRGWLEWNATFALDTLYYGEYMNYGPGGAVGQRVTWP 550
           LGRPWKLYSR VYM SY+G HVHPRGWLEWN TFALDTLYYGEYMNYGPGGAVGQRV WP
Sbjct: 511 LGRPWKLYSRTVYMLSYIGDHVHPRGWLEWNTTFALDTLYYGEYMNYGPGGAVGQRVKWP 570

Query: 551 GYRVITSRVEASQFTVAQFIYGSSWLPSTGVAFLGGLNL 582
           GYRV+TS +EAS+FTVAQFIYGSSWLPSTGV+FL GL+L
Sbjct: 571 GYRVVTSTIEASKFTVAQFIYGSSWLPSTGVSFLAGLSL 605