Cucsa.181720 (gene) Cucumber (Gy14) v1

BLAST of Cucsa.181720 vs. Swiss-Prot
Match: PME28_ARATH (Putative pectinesterase/pectinesterase inhibitor 28 OS=Arabidopsis thaliana GN=PME28 PE=2 SV=1)

HSP 1 Score: 657.5 bits (1695), Expect = 1.3e-187
Identity = 311/572 (54.37%), Postives = 431/572 (75.35%), Query Frame = 1

Query: 2   GYKDDDSQNKKRYAIIGVSSMLLVAMVVAVTVGVNLNQDETSDPATGNKSHEISSSMKAI 61
           GY D+D++ KKRY II +SS+LL++MVVAVT+GV++N+ + +         EI++S+KAI
Sbjct: 4   GYDDEDAKRKKRYVIISISSVLLISMVVAVTIGVSVNKSDNAG------DEEITTSVKAI 63

Query: 62  KAICQPTDYKQECVASLKATGNNSSDPKELVQAGFKAAMKLIQAAANKSVALNQLEKDPR 121
           K +C PTDYK+ C  +L+    ++SDP ELV+  F A MK I   A KS  + +L+KDPR
Sbjct: 64  KDVCAPTDYKETCEDTLRKDAKDTSDPLELVKTAFNATMKQISDVAKKSQTMIELQKDPR 123

Query: 122 ASKALAGCKELMDFAIDELKYSMNKLGEFDISKLDEMLIDIRIWLSATITYQETCLDGFA 181
           A  AL  CKELMD+AI EL  S  +LG+F+  K+DE L+ +RIWLSATI++++TCLDGF 
Sbjct: 124 AKMALDQCKELMDYAIGELSKSFEELGKFEFHKVDEALVKLRIWLSATISHEQTCLDGFQ 183

Query: 182 NTTGNAAEKMKKALKTSMKLSSNGLAMVSQISSMLSELQIPGISRRRLLEIPVLGHDDYP 241
            T GNA E +KKALKT+++L+ NGLAMV+++S+ L ++QIP ++ RRLL        ++P
Sbjct: 184 GTQGNAGETIKKALKTAVQLTHNGLAMVTEMSNYLGQMQIPEMNSRRLLS------QEFP 243

Query: 242 DWANPGMRRLLAAG-SKVKPNVVVAKDGSGQFKTIQEAIDQVPKRKNNATYVIHIKAGVY 301
            W +   RRLL A  S+VKP++VVA+DGSGQ+KTI EA++ VPK+KN  T+V+HIK G+Y
Sbjct: 244 SWMDARARRLLNAPMSEVKPDIVVAQDGSGQYKTINEALNFVPKKKNT-TFVVHIKEGIY 303

Query: 302 QEYVLVKKTLTHLMLIGDGPKKTIITGNKNFIDGTPTFKTATVAVTAEHFMARDIGFENT 361
           +EYV V +++THL+ IGDGP KT+I+G+K++ DG  T+KTATVA+  +HF+A++I FENT
Sbjct: 304 KEYVQVNRSMTHLVFIGDGPDKTVISGSKSYKDGITTYKTATVAIVGDHFIAKNIAFENT 363

Query: 362 AGPQKHQAVALRVQADKAVFYNCEMHGYQDTLYVHTMRQFYRDCTVSGTIDFIFGDAAAI 421
           AG  KHQAVA+RV AD+++FYNC+  GYQDTLY H+ RQFYRDCT+SGTIDF+FGDAAA+
Sbjct: 364 AGAIKHQAVAIRVLADESIFYNCKFDGYQDTLYAHSHRQFYRDCTISGTIDFLFGDAAAV 423

Query: 422 FQSCTFLVRKPLPNQQCIVTAHGRKERRQPSALIIQNCSFKPHADLVPVQKQFRSFLGRP 481
           FQ+CT LVRKPL NQ C +TAHGRK+ R+ +  ++Q C+     D + V++Q +++LGRP
Sbjct: 424 FQNCTLLVRKPLLNQACPITAHGRKDPRESTGFVLQGCTIVGEPDYLAVKEQSKTYLGRP 483

Query: 482 WKEYSRTIIMESYIGDLIQPEGWLPWAGDWGLRTCFYTEYNNYGPGSDKSKRVKWRGIKN 541
           WKEYSRTIIM ++I D + PEGW PW G++GL T FY+E  N GPG+  +KRV W GIK 
Sbjct: 484 WKEYSRTIIMNTFIPDFVPPEGWQPWLGEFGLNTLFYSEVQNTGPGAAITKRVTWPGIKK 543

Query: 542 ITPQHAVDFTPGRFLKGDRWIKPTGVPYVSGL 573
           ++ +  + FTP ++++GD WI   GVPY+ GL
Sbjct: 544 LSDEEILKFTPAQYIQGDAWIPGKGVPYILGL 562

BLAST of Cucsa.181720 vs. Swiss-Prot
Match: PME21_ARATH (Probable pectinesterase/pectinesterase inhibitor 21 OS=Arabidopsis thaliana GN=PME21 PE=2 SV=2)

HSP 1 Score: 652.5 bits (1682), Expect = 4.3e-186
Identity = 311/574 (54.18%), Postives = 428/574 (74.56%), Query Frame = 1

Query: 2   GYKDDDSQNKKRYAIIGVSSMLLVAMVVAVTVGVNLNQDETSDPATGNKSHEISSSMKAI 61
           GY DD+S+ K+RY +I +SS+LL++MVVAVTVGV+LN+ +           E+++S+KA+
Sbjct: 4   GY-DDESKRKRRYIVITISSVLLISMVVAVTVGVSLNKHDGDSKGKA----EVNASVKAV 63

Query: 62  KAICQPTDYKQECVASLKATGNNSSDPKELVQAGFKAAMKLIQAAANKSVALNQLEKDPR 121
           K +C PTDY++ C  +L   G N++DP ELV+  F   MK I  AA KS  + +L+KD R
Sbjct: 64  KDVCAPTDYRKTCEDTLIKNGKNTTDPMELVKTAFNVTMKQITDAAKKSQTIMELQKDSR 123

Query: 122 ASKALAGCKELMDFAIDELKYSMNKLGEFDISKLDEMLIDIRIWLSATITYQETCLDGFA 181
              AL  CKELMD+A+DEL  S  +LG+F+   LDE LI++RIWLSA I+++ETCL+GF 
Sbjct: 124 TRMALDQCKELMDYALDELSNSFEELGKFEFHLLDEALINLRIWLSAAISHEETCLEGFQ 183

Query: 182 NTTGNAAEKMKKALKTSMKLSSNGLAMVSQISSMLSELQIPGISRRRLLEIPVLGHDDYP 241
            T GNA E MKKALKT+++L+ NGLA++S++S+ + ++QIPG++ RRLL       + +P
Sbjct: 184 GTQGNAGETMKKALKTAIELTHNGLAIISEMSNFVGQMQIPGLNSRRLLA------EGFP 243

Query: 242 DWANPGMRRLL---AAGSKVKPNVVVAKDGSGQFKTIQEAIDQVPKRKNNATYVIHIKAG 301
            W +   R+LL   AA S VKP++VVA+DGSGQ+KTI EA+  VPK K N T+V+HIKAG
Sbjct: 244 SWVDQRGRKLLQAAAAYSDVKPDIVVAQDGSGQYKTINEALQFVPK-KRNTTFVVHIKAG 303

Query: 302 VYQEYVLVKKTLTHLMLIGDGPKKTIITGNKNFIDGTPTFKTATVAVTAEHFMARDIGFE 361
           +Y+EYV V KT++HL+ IGDGP KTII+GNKN+ DG  T++TATVA+   +F+A++IGFE
Sbjct: 304 LYKEYVQVNKTMSHLVFIGDGPDKTIISGNKNYKDGITTYRTATVAIVGNYFIAKNIGFE 363

Query: 362 NTAGPQKHQAVALRVQADKAVFYNCEMHGYQDTLYVHTMRQFYRDCTVSGTIDFIFGDAA 421
           NTAG  KHQAVA+RVQ+D+++F+NC   GYQDTLY H+ RQF+RDCT+SGTIDF+FGDAA
Sbjct: 364 NTAGAIKHQAVAVRVQSDESIFFNCRFDGYQDTLYTHSHRQFFRDCTISGTIDFLFGDAA 423

Query: 422 AIFQSCTFLVRKPLPNQQCIVTAHGRKERRQPSALIIQNCSFKPHADLVPVQKQFRSFLG 481
           A+FQ+CT LVRKPLPNQ C +TAHGRK+ R+ +  + Q C+     D + V++  +++LG
Sbjct: 424 AVFQNCTLLVRKPLPNQACPITAHGRKDPRESTGFVFQGCTIAGEPDYLAVKETSKAYLG 483

Query: 482 RPWKEYSRTIIMESYIGDLIQPEGWLPWAGDWGLRTCFYTEYNNYGPGSDKSKRVKWRGI 541
           RPWKEYSRTIIM ++I D +QP+GW PW GD+GL+T FY+E  N GPGS  + RV W GI
Sbjct: 484 RPWKEYSRTIIMNTFIPDFVQPQGWQPWLGDFGLKTLFYSEVQNTGPGSALANRVTWAGI 543

Query: 542 KNITPQHAVDFTPGRFLKGDRWIKPTGVPYVSGL 573
           K ++ +  + FTP ++++GD WI   GVPY +GL
Sbjct: 544 KTLSEEDILKFTPAQYIQGDDWIPGKGVPYTTGL 565

BLAST of Cucsa.181720 vs. Swiss-Prot
Match: PME58_ARATH (Probable pectinesterase/pectinesterase inhibitor 58 OS=Arabidopsis thaliana GN=PME58 PE=2 SV=1)

HSP 1 Score: 539.7 bits (1389), Expect = 4.0e-152
Identity = 274/575 (47.65%), Postives = 381/575 (66.26%), Query Frame = 1

Query: 6   DDSQNKKRYAIIGVSSMLLVAMVVAV--TVGVNLNQDETSDPATGNKSHEISSSMKAIKA 65
           D    KK+  I GV + LLV MVVAV  T   N +  E   P       +I ++  A++A
Sbjct: 4   DGELKKKKCIIAGVITALLVLMVVAVGITTSRNTSHSEKIVPV------QIKTATTAVEA 63

Query: 66  ICQPTDYKQECVASLKATGNNSSDPKELVQAGFKAAMKLIQAAANK-SVALNQLEKDPRA 125
           +C PTDYK+ CV SL     +S+ P +L++ GF   ++ I+ +  K SV L     + + 
Sbjct: 64  VCAPTDYKETCVNSLMKASPDSTQPLDLIKLGFNVTIRSIEDSIKKASVELTAKAANDKD 123

Query: 126 SK-ALAGCKELMDFAIDELKYSMNKLGEFDISKLDEMLIDIRIWLSATITYQETCLDGFA 185
           +K AL  C++LM+ A D+LK  ++    F I ++++ + D+R+WLS +I YQ+TC+D F 
Sbjct: 124 TKGALELCEKLMNDATDDLKKCLDNFDGFSIPQIEDFVEDLRVWLSGSIAYQQTCMDTFE 183

Query: 186 NTTGNAAEKMKKALKTSMKLSSNGLAMVSQISSMLSELQIPGIS------RRRLLEIPVL 245
            T    ++ M+K  KTS +L+SNGLAM++ IS++L E  + G++       R+LL     
Sbjct: 184 ETNSKLSQDMQKIFKTSRELTSNGLAMITNISNLLGEFNVTGVTGDLGKYARKLLS---- 243

Query: 246 GHDDYPDWANPGMRRLLAAGSKVKPNVVVAKDGSGQFKTIQEAIDQVPKRKNNATYVIHI 305
             D  P W  P  RRL+A    VK NVVVA DGSGQ+KTI EA++ VPK  N   +VI+I
Sbjct: 244 AEDGIPSWVGPNTRRLMATKGGVKANVVVAHDGSGQYKTINEALNAVPKA-NQKPFVIYI 303

Query: 306 KAGVYQEYVLVKKTLTHLMLIGDGPKKTIITGNKNFIDG-TPTFKTATVAVTAEHFMARD 365
           K GVY E V V K +TH+  IGDGP KT ITG+ N+  G   T+ TATVA+  ++F A++
Sbjct: 304 KQGVYNEKVDVTKKMTHVTFIGDGPTKTKITGSLNYYIGKVKTYLTATVAINGDNFTAKN 363

Query: 366 IGFENTAGPQKHQAVALRVQADKAVFYNCEMHGYQDTLYVHTMRQFYRDCTVSGTIDFIF 425
           IGFENTAGP+ HQAVALRV AD AVFYNC++ GYQDTLYVH+ RQF+RDCTVSGT+DFIF
Sbjct: 364 IGFENTAGPEGHQAVALRVSADLAVFYNCQIDGYQDTLYVHSHRQFFRDCTVSGTVDFIF 423

Query: 426 GDAAAIFQSCTFLVRKPLPNQQCIVTAHGRKERRQPSALIIQNCSFKPHADLVPVQKQFR 485
           GD   + Q+C  +VRKP+ +Q C++TA GR ++R+ + L++QNC        +PV+   +
Sbjct: 424 GDGIVVLQNCNIVVRKPMKSQSCMITAQGRSDKRESTGLVLQNCHITGEPAYIPVKSINK 483

Query: 486 SFLGRPWKEYSRTIIMESYIGDLIQPEGWLPWAGDWGLRTCFYTEYNNYGPGSDKSKRVK 545
           ++LGRPWKE+SRTIIM + I D+I P GWLPW GD+ L T +Y EY N GPGS++++RVK
Sbjct: 484 AYLGRPWKEFSRTIIMGTTIDDVIDPAGWLPWNGDFALNTLYYAEYENNGPGSNQAQRVK 543

Query: 546 WRGIKNITPQHAVDFTPGRFLKGDRWIKPTGVPYV 570
           W GIK ++P+ A+ FTP RFL+G+ WI P  VPY+
Sbjct: 544 WPGIKKLSPKQALRFTPARFLRGNLWIPPNRVPYM 567

BLAST of Cucsa.181720 vs. Swiss-Prot
Match: PME23_ARATH (Probable pectinesterase/pectinesterase inhibitor 23 OS=Arabidopsis thaliana GN=PME23 PE=2 SV=3)

HSP 1 Score: 510.4 bits (1313), Expect = 2.6e-143
Identity = 264/577 (45.75%), Postives = 370/577 (64.12%), Query Frame = 1

Query: 7   DSQNKKRYAIIGVSSMLLVAMVVAVTVGVNLNQDETSDPATGNKSHEISSSMKAIKAICQ 66
           D   KK++ + G  S  LV MVV+V V        TS  +  +  + I  + KA++A+C 
Sbjct: 4   DGDKKKKFIVAGSVSGFLVIMVVSVAV-------VTSKHSPKDDENHIRKTTKAVQAVCA 63

Query: 67  PTDYKQECVASLKATGNNSSDPKELVQAGFKAAMKLIQAAANKSVA--LNQLEKDPRASK 126
           PTD+K  CV SL     +S DP +L++ GFK  +K I  +  K+      + +K+P A  
Sbjct: 64  PTDFKDTCVNSLMGASPDSDDPVDLIKLGFKVTIKSINESLEKASGDIKAKADKNPEAKG 123

Query: 127 ALAGCKELMDFAIDELKYSMNKLGEFDISKLDEMLIDIRIWLSATITYQETCLDGFANTT 186
           A   C++LM  AID+LK  M+    F + +++  + D+R+WLS +I +Q+TC+D F    
Sbjct: 124 AFELCEKLMIDAIDDLKKCMDH--GFSVDQIEVFVEDLRVWLSGSIAFQQTCMDSFGEIK 183

Query: 187 GNAAEKMKKALKTSMKLSSNGLAMVSQISSMLSELQIPGIS------RRRLLEIPVLGHD 246
            N  + M K  KTS +LSSN LAMV++IS+++    + G++       R+LL       D
Sbjct: 184 SNLMQDMLKIFKTSRELSSNSLAMVTRISTLIPNSNLTGLTGALAKYARKLLST----ED 243

Query: 247 DYPDWANPGMRRLLAAGS----KVKPNVVVAKDGSGQFKTIQEAIDQVPKRKNNATYVIH 306
             P W  P  RRL+AA       VK N VVA+DG+GQFKTI +A++ VPK  N   ++IH
Sbjct: 244 SIPTWVGPEARRLMAAQGGGPGPVKANAVVAQDGTGQFKTITDALNAVPKG-NKVPFIIH 303

Query: 307 IKAGVYQEYVLVKKTLTHLMLIGDGPKKTIITGNKNF-IDGTPTFKTATVAVTAEHFMAR 366
           IK G+Y+E V V K + H+  IGDGP KT+ITG+ NF I    TF TAT+ +  +HF A+
Sbjct: 304 IKEGIYKEKVTVTKKMPHVTFIGDGPNKTLITGSLNFGIGKVKTFLTATITIEGDHFTAK 363

Query: 367 DIGFENTAGPQKHQAVALRVQADKAVFYNCEMHGYQDTLYVHTMRQFYRDCTVSGTIDFI 426
           +IG ENTAGP+  QAVALRV AD AVF++C++ G+QDTLYVH+ RQFYRDCTVSGT+DFI
Sbjct: 364 NIGIENTAGPEGGQAVALRVSADYAVFHSCQIDGHQDTLYVHSHRQFYRDCTVSGTVDFI 423

Query: 427 FGDAAAIFQSCTFLVRKPLPNQQCIVTAHGRKERRQPSALIIQNCSFKPHADLVPVQKQF 486
           FGDA  I Q+C  +VRKP   Q C+VTA GR   R+ + L++  C        +P++   
Sbjct: 424 FGDAKCILQNCKIVVRKPNKGQTCMVTAQGRSNVRESTGLVLHGCHITGDPAYIPMKSVN 483

Query: 487 RSFLGRPWKEYSRTIIMESYIGDLIQPEGWLPWAGDWGLRTCFYTEYNNYGPGSDKSKRV 546
           +++LGRPWKE+SRTIIM++ I D+I P GWLPW+GD+ L+T +Y E+ N GPGS++++RV
Sbjct: 484 KAYLGRPWKEFSRTIIMKTTIDDVIDPAGWLPWSGDFALKTLYYAEHMNTGPGSNQAQRV 543

Query: 547 KWRGIKNITPQHAVDFTPGRFLKGDRWIKPTGVPYVS 571
           KW GIK +TPQ A+ +T  RFL+GD WI  T VPY +
Sbjct: 544 KWPGIKKLTPQDALLYTGDRFLRGDTWIPQTQVPYTA 566

BLAST of Cucsa.181720 vs. Swiss-Prot
Match: PME_MEDSA (Pectinesterase/pectinesterase inhibitor OS=Medicago sativa PE=2 SV=1)

HSP 1 Score: 453.4 bits (1165), Expect = 3.8e-126
Identity = 226/449 (50.33%), Postives = 300/449 (66.82%), Query Frame = 1

Query: 129 CKELMDFAIDELKYSMNKLGEFDISKLDEMLIDIRIWLSATITYQETCLDGFANTTGNAA 188
           C E++D+A+D +  S+  L +FD  KL E   D+++WL+ T+++Q+TCLDGFANTT  A 
Sbjct: 4   CNEVLDYAVDGIHKSVGTLDQFDFHKLSEYAFDLKVWLTGTLSHQQTCLDGFANTTTKAG 63

Query: 189 EKMKKALKTSMKLSSNGLAMVSQISSMLSELQIPGISRRRLLEIPVLGHDDYPDWANPGM 248
           E M K LKTSM+LSSN + M+  +S +L        S  R L    L  D  P W N G 
Sbjct: 64  ETMTKVLKTSMELSSNAIDMMDAVSRILKGFDTSQYSVSRRL----LSDDGIPSWVNDGH 123

Query: 249 RRLLAAGSKVKPNVVVAKDGSGQFKTIQEAIDQVPKRKNNATYVIHIKAGVYQEYVLVKK 308
           RRLLA G+ V+PN VVA+DGSGQFKT+ +A+  VP  KN   +VIH+KAGVY+E V V K
Sbjct: 124 RRLLAGGN-VQPNAVVAQDGSGQFKTLTDALKTVPP-KNAVPFVIHVKAGVYKETVNVAK 183

Query: 309 TLTHLMLIGDGPKKTIITGNKNFIDGTPTFKTATVAVTAEHFMARDIGFENTAGPQKHQA 368
            + ++ +IGDGP KT  TG+ N+ DG  T+ TAT  V   +FMA+DIGFENTAG  KHQA
Sbjct: 184 EMNYVTVIGDGPTKTKFTGSLNYADGINTYNTATFGVNGANFMAKDIGFENTAGTGKHQA 243

Query: 369 VALRVQADKAVFYNCEMHGYQDTLYVHTMRQFYRDCTVSGTIDFIFGDAAAIFQSCTFLV 428
           VALRV AD+A+FYNC+M G+QDTLYV + RQFYRDC++SGTIDF+FG+   +FQ+C  + 
Sbjct: 244 VALRVTADQAIFYNCQMDGFQDTLYVQSQRQFYRDCSISGTIDFVFGERFGVFQNCKLVC 303

Query: 429 RKPLPNQQCIVTAHGRKERRQPSALIIQNCSFKPHADLVPVQKQFRSFLGRPWKEYSRTI 488
           R P   QQC+VTA GR+++   SAL+ Q+  F     L  V  +  S+LGRPWK+YS+ +
Sbjct: 304 RLPAKGQQCLVTAGGREKQNSASALVFQSSHFTGEPALTSVTPKV-SYLGRPWKQYSKVV 363

Query: 489 IMESYIGDLIQPEGWLPWAGDWGLRTCFYTEYNNYGPGSDKSKRVKWRGIKNITPQHAVD 548
           IM+S I  +  PEG++PW G     TC Y EYNN GPG+D + RVKW G+K +T   A +
Sbjct: 364 IMDSTIDAIFVPEGYMPWMGSAFKETCTYYEYNNKGPGADTNLRVKWHGVKVLTSNVAAE 423

Query: 549 FTPGRFLK------GDRWIKPTGVPYVSG 572
           + PG+F +       D WI  +GVPY  G
Sbjct: 424 YYPGKFFEIVNATARDTWIVKSGVPYSLG 445

BLAST of Cucsa.181720 vs. TrEMBL
Match: A0A0A0LYF5_CUCSA (Pectinesterase OS=Cucumis sativus GN=Csa_1G569220 PE=4 SV=1)

HSP 1 Score: 1164.1 bits (3010), Expect = 0.0e+00
Identity = 576/576 (100.00%), Postives = 576/576 (100.00%), Query Frame = 1

Query: 1   MGYKDDDSQNKKRYAIIGVSSMLLVAMVVAVTVGVNLNQDETSDPATGNKSHEISSSMKA 60
           MGYKDDDSQNKKRYAIIGVSSMLLVAMVVAVTVGVNLNQDETSDPATGNKSHEISSSMKA
Sbjct: 1   MGYKDDDSQNKKRYAIIGVSSMLLVAMVVAVTVGVNLNQDETSDPATGNKSHEISSSMKA 60

Query: 61  IKAICQPTDYKQECVASLKATGNNSSDPKELVQAGFKAAMKLIQAAANKSVALNQLEKDP 120
           IKAICQPTDYKQECVASLKATGNNSSDPKELVQAGFKAAMKLIQAAANKSVALNQLEKDP
Sbjct: 61  IKAICQPTDYKQECVASLKATGNNSSDPKELVQAGFKAAMKLIQAAANKSVALNQLEKDP 120

Query: 121 RASKALAGCKELMDFAIDELKYSMNKLGEFDISKLDEMLIDIRIWLSATITYQETCLDGF 180
           RASKALAGCKELMDFAIDELKYSMNKLGEFDISKLDEMLIDIRIWLSATITYQETCLDGF
Sbjct: 121 RASKALAGCKELMDFAIDELKYSMNKLGEFDISKLDEMLIDIRIWLSATITYQETCLDGF 180

Query: 181 ANTTGNAAEKMKKALKTSMKLSSNGLAMVSQISSMLSELQIPGISRRRLLEIPVLGHDDY 240
           ANTTGNAAEKMKKALKTSMKLSSNGLAMVSQISSMLSELQIPGISRRRLLEIPVLGHDDY
Sbjct: 181 ANTTGNAAEKMKKALKTSMKLSSNGLAMVSQISSMLSELQIPGISRRRLLEIPVLGHDDY 240

Query: 241 PDWANPGMRRLLAAGSKVKPNVVVAKDGSGQFKTIQEAIDQVPKRKNNATYVIHIKAGVY 300
           PDWANPGMRRLLAAGSKVKPNVVVAKDGSGQFKTIQEAIDQVPKRKNNATYVIHIKAGVY
Sbjct: 241 PDWANPGMRRLLAAGSKVKPNVVVAKDGSGQFKTIQEAIDQVPKRKNNATYVIHIKAGVY 300

Query: 301 QEYVLVKKTLTHLMLIGDGPKKTIITGNKNFIDGTPTFKTATVAVTAEHFMARDIGFENT 360
           QEYVLVKKTLTHLMLIGDGPKKTIITGNKNFIDGTPTFKTATVAVTAEHFMARDIGFENT
Sbjct: 301 QEYVLVKKTLTHLMLIGDGPKKTIITGNKNFIDGTPTFKTATVAVTAEHFMARDIGFENT 360

Query: 361 AGPQKHQAVALRVQADKAVFYNCEMHGYQDTLYVHTMRQFYRDCTVSGTIDFIFGDAAAI 420
           AGPQKHQAVALRVQADKAVFYNCEMHGYQDTLYVHTMRQFYRDCTVSGTIDFIFGDAAAI
Sbjct: 361 AGPQKHQAVALRVQADKAVFYNCEMHGYQDTLYVHTMRQFYRDCTVSGTIDFIFGDAAAI 420

Query: 421 FQSCTFLVRKPLPNQQCIVTAHGRKERRQPSALIIQNCSFKPHADLVPVQKQFRSFLGRP 480
           FQSCTFLVRKPLPNQQCIVTAHGRKERRQPSALIIQNCSFKPHADLVPVQKQFRSFLGRP
Sbjct: 421 FQSCTFLVRKPLPNQQCIVTAHGRKERRQPSALIIQNCSFKPHADLVPVQKQFRSFLGRP 480

Query: 481 WKEYSRTIIMESYIGDLIQPEGWLPWAGDWGLRTCFYTEYNNYGPGSDKSKRVKWRGIKN 540
           WKEYSRTIIMESYIGDLIQPEGWLPWAGDWGLRTCFYTEYNNYGPGSDKSKRVKWRGIKN
Sbjct: 481 WKEYSRTIIMESYIGDLIQPEGWLPWAGDWGLRTCFYTEYNNYGPGSDKSKRVKWRGIKN 540

Query: 541 ITPQHAVDFTPGRFLKGDRWIKPTGVPYVSGLTRTG 577
           ITPQHAVDFTPGRFLKGDRWIKPTGVPYVSGLTRTG
Sbjct: 541 ITPQHAVDFTPGRFLKGDRWIKPTGVPYVSGLTRTG 576

BLAST of Cucsa.181720 vs. TrEMBL
Match: W9S2R1_9ROSA (Pectinesterase OS=Morus notabilis GN=L484_001919 PE=4 SV=1)

HSP 1 Score: 788.1 bits (2034), Expect = 7.2e-225
Identity = 383/576 (66.49%), Postives = 471/576 (81.77%), Query Frame = 1

Query: 6   DDSQNKKRYAIIGVSSMLLVAMVVAVTVGVNLNQDETSDPATGNK--SHEISSSMKAIKA 65
           DD + +KRYA+IG+SS+LLVAMV+AVTVGV+LN  ++S PA+ NK  SHE+S+SMKAIK+
Sbjct: 5   DDGEKRKRYAVIGISSVLLVAMVIAVTVGVSLNNGQSS-PASSNKDGSHEVSASMKAIKS 64

Query: 66  ICQPTDYKQECVASLKATGNNSSDPKELVQAGFKAAMKLIQAAANKSVALNQLEKDPRAS 125
           ICQPTDYK+EC+ SL +   N++DPK+L+QAGFK AMK +  AA KSV L  LEKDPRAS
Sbjct: 65  ICQPTDYKEECINSLSSESGNTTDPKKLIQAGFKVAMKYLSEAAKKSVVLQDLEKDPRAS 124

Query: 126 KALAGCKELMDFAIDELKYSMNKLGEFDISKLDEMLIDIRIWLSATITYQETCLDGFANT 185
           KAL  CK+LM+ +ID+LK+S++KLGEFDISK D+ML+D++IWLSAT+TYQETCLDGF NT
Sbjct: 125 KALGHCKQLMENSIDDLKHSVDKLGEFDISKFDQMLMDLKIWLSATVTYQETCLDGFQNT 184

Query: 186 TGNAAEKMKKALKTSMKLSSNGLAMVSQISSMLSELQIPGISRRRLL-----EIPVLGHD 245
           TG AAEKMKKALKTSM+LSSNGL MVS+ISS+ S+LQ+  + RR        E+PVLGH 
Sbjct: 185 TGEAAEKMKKALKTSMRLSSNGLTMVSEISSVFSDLQLSSMGRRLFSDEEESELPVLGHS 244

Query: 246 DYPDWANPGMRRLLAAG-SKVKPNVVVAKDGSGQFKTIQEAIDQVPKRKNNATYVIHIKA 305
           D  +W   G+RRL+A   +KVKPN+VVAKDGSG++ TI EA+  +P+R  N T+VI+IK 
Sbjct: 245 DEAEWFGGGVRRLMAVHPTKVKPNIVVAKDGSGKYTTINEALKDIPRRSEN-TFVIYIKE 304

Query: 306 GVYQEYVLVKKTLTHLMLIGDGPKKTIITGNKNFIDGTPTFKTATVAVTAEHFMARDIGF 365
           GVY+EYV +++ +T+L++IGDG  KT ITG+KNFIDG PT  TATV V  +HF ARDIGF
Sbjct: 305 GVYKEYVTIERNMTNLLIIGDGANKTRITGDKNFIDGFPTSLTATVVVLGDHFRARDIGF 364

Query: 366 ENTAGPQKHQAVALRVQADKAVFYNCEMHGYQDTLYVHTMRQFYRDCTVSGTIDFIFGDA 425
           ENTAG  KHQAVALRV AD+A+FY C M GYQDTLY H  RQFYRDC VSGTIDF+FGDA
Sbjct: 365 ENTAGAHKHQAVALRVNADEAIFYRCAMDGYQDTLYTHAKRQFYRDCNVSGTIDFVFGDA 424

Query: 426 AAIFQSCTFLVRKPLPNQQCIVTAHGRKERRQPSALIIQNCSFKPHADLVPVQKQFRSFL 485
           AA+FQ+C F+VR+P+ NQQCIVTA GRKERRQPSA+IIQN +F    D  PV+ +  ++L
Sbjct: 425 AAVFQNCRFIVRRPMDNQQCIVTAQGRKERRQPSAIIIQNGTFVADPDYYPVRFELPAYL 484

Query: 486 GRPWKEYSRTIIMESYIGDLIQPEGWLPWAGDWGLRTCFYTEYNNYGPGS-DKSKRVKWR 545
           GRPWKE+SRTIIMES+I DLIQP GWLPWAGD+GLRTCFYTE+ N GPG+ + S+R KW+
Sbjct: 485 GRPWKEFSRTIIMESFIDDLIQPRGWLPWAGDFGLRTCFYTEFGNRGPGAVEPSRRAKWK 544

Query: 546 GIKNITPQHAVDFTPGRFLKGDRWIKPTGVPYVSGL 573
           G+KNIT +HA DFTPG F KGD WIK TG+PYVSG+
Sbjct: 545 GVKNITAKHARDFTPGSFFKGDWWIKRTGIPYVSGM 578

BLAST of Cucsa.181720 vs. TrEMBL
Match: B9RFE0_RICCO (Pectinesterase OS=Ricinus communis GN=RCOM_1433930 PE=4 SV=1)

HSP 1 Score: 751.5 bits (1939), Expect = 7.5e-214
Identity = 371/578 (64.19%), Postives = 459/578 (79.41%), Query Frame = 1

Query: 6   DDSQNKKRYAIIGVSSMLLVAMVVAVTVGVNLNQD-ETSDPATG--NKSHEISSSMKAIK 65
           +DS+ KKR AIIGVSS LLVAMVVAVTV +  N D E+ D   G  +KS E+S+SMKAIK
Sbjct: 5   EDSKRKKRLAIIGVSSFLLVAMVVAVTVSIGFNNDGESEDDINGKNHKSKEVSASMKAIK 64

Query: 66  AICQPTDYKQECVASLKATGNNSSDPKELVQAGFKAAMKLIQAAANKSVALNQLEKDPRA 125
            +CQPT YKQ C  SL  +  N++DPKEL++  FK A K I +A+ KS+ L +LEKDPR 
Sbjct: 65  TLCQPTYYKQTCERSLAKSAGNTTDPKELIKIAFKLAEKQIDSASKKSLTLLELEKDPRT 124

Query: 126 SKALAGCKELMDFAIDELKYSMNKLGEFDISKLDEMLIDIRIWLSATITYQETCLDGFAN 185
             AL  CKELM  +I+EL+ S+ K+ +FD S+LDE++ DI+ WLSA ITY+ETCLD F N
Sbjct: 125 RGALNSCKELMTMSINELRSSLEKVADFDFSQLDELMADIKTWLSAAITYEETCLDAFEN 184

Query: 186 TTGNAAEKMKKALKTSMKLSSNGLAMVSQISSMLSELQIPGISRRRLLE-IPVLGHDD-- 245
           TT NA EKMKKALKT+M++SSNGL +VS ISS+L++LQIPG+SRR L + IPV GH D  
Sbjct: 185 TTTNAGEKMKKALKTAMEMSSNGLDIVSGISSVLTDLQIPGVSRRLLQDDIPVAGHGDIS 244

Query: 246 --YPDWANPGMRRLLAAG-SKVKPNVVVAKDGSGQFKTIQEAIDQVPKRKNNATYVIHIK 305
             +P W +PG RRLL+A  S +KP++VVAKDGSG +KTI EA+ Q+PK K+N T+V++IK
Sbjct: 245 QAFPAWIDPGTRRLLSAPPSNIKPDLVVAKDGSGDYKTILEALPQIPK-KSNETFVLYIK 304

Query: 306 AGVYQEYVLVKKTLTHLMLIGDGPKKTIITGNKNFIDGTPTFKTATVAVTAEHFMARDIG 365
            G+Y+EYV   +++T+L++IGDGP KT ITG+KNF+DG  T++TATVAV  ++F+AR+IG
Sbjct: 305 EGIYEEYVEFNRSMTNLVVIGDGPDKTRITGSKNFVDGINTYRTATVAVIGDNFVARNIG 364

Query: 366 FENTAGPQKHQAVALRVQADKAVFYNCEMHGYQDTLYVHTMRQFYRDCTVSGTIDFIFGD 425
           FEN+AG  KHQAVALRV +D AVFYNC M GYQDTLY H  RQFYRDCTVSGTIDF+FGD
Sbjct: 365 FENSAGAIKHQAVALRVSSDYAVFYNCSMDGYQDTLYTHAKRQFYRDCTVSGTIDFVFGD 424

Query: 426 AAAIFQSCTFLVRKPLPNQQCIVTAHGRKERRQPSALIIQNCSFKPHADLVPVQKQFRSF 485
           A  +FQ+CTFLVRKPL NQQCIVTA GRK RRQPSA+IIQN +   H +L PV+ Q++S+
Sbjct: 425 APVVFQNCTFLVRKPLENQQCIVTAQGRKARRQPSAIIIQNSTITAHPELEPVKDQYKSY 484

Query: 486 LGRPWKEYSRTIIMESYIGDLIQPEGWLPWAGDWGLRTCFYTEYNNYGPGSDKSKRVKWR 545
           LGRPWKE+SRTIIME++I DLIQPEGW PW G +GL+TC+Y EYNNYGPGSD   RVKW 
Sbjct: 485 LGRPWKEFSRTIIMETFIDDLIQPEGWSPWFGSFGLKTCWYGEYNNYGPGSDMKNRVKWN 544

Query: 546 GIKNITPQHAVDFTPGRFLKGDRWIKPTGVPYVSGLTR 575
           GIK ++ QHA+DFTPGRFL+GD WIKPTGVPY   LTR
Sbjct: 545 GIKPVSRQHAIDFTPGRFLRGDSWIKPTGVPYAPYLTR 581

BLAST of Cucsa.181720 vs. TrEMBL
Match: A0A067JV77_JATCU (Pectinesterase OS=Jatropha curcas GN=JCGZ_23282 PE=4 SV=1)

HSP 1 Score: 751.1 bits (1938), Expect = 9.8e-214
Identity = 366/579 (63.21%), Postives = 457/579 (78.93%), Query Frame = 1

Query: 7   DSQNKKRYAIIGVSSMLLVAMVVAVTVGVNLNQDETSDPATGNKSH----EISSSMKAIK 66
           D  N KR AIIG+SS+LLVAMVVAVT+GV  +++  S+  TG KSH    EIS+SMKAIK
Sbjct: 3   DDANNKRLAIIGISSILLVAMVVAVTIGVGFSENGESEDITG-KSHKSTGEISASMKAIK 62

Query: 67  AICQPTDYKQECVASLKATGNNSSDPKELVQAGFKAAMKLIQAAANKSVALNQLEKDPRA 126
           +ICQP DYK+ C  SL+ +  N++DPKEL++A FK A K I+ AA KS  L +LEKDPR 
Sbjct: 63  SICQPADYKKTCEKSLEKSAGNTTDPKELIKAAFKIAQKKIREAAKKSATLEELEKDPRT 122

Query: 127 SKALAGCKELMDFAIDELKYSMNKLGEFDISKLDEMLIDIRIWLSATITYQETCLDGFAN 186
             AL  CKELMD +I EL+ S +K+ +FDI+ LD+ + D+R WLSA ITY+ TCLDGF N
Sbjct: 123 RDALQSCKELMDMSISELQNSFDKVADFDITMLDDFMADLRTWLSAVITYEGTCLDGFEN 182

Query: 187 TTGNAAEKMKKALKTSMKLSSNGLAMVSQISSMLSELQIPGISRRRLLEIPVLGHDD--- 246
           TT +A EKMKKA+K ++++S+NGL ++  +SS L++LQI G++RR L E  VLGHDD   
Sbjct: 183 TTSDAGEKMKKAMKAAIEMSTNGLDIIDGLSSALADLQITGVNRRLLQEDEVLGHDDVPS 242

Query: 247 -YPDWANPGMRRLLAAG-SKVKPNVVVAKDGSGQFKTIQEAIDQVPKRKNNAT--YVIHI 306
            +P W + G RRLLAA  SK+KPN+ VAKDGSG FKTI+ A+D +PK K  +T  +V++I
Sbjct: 243 KFPSWVDAGTRRLLAAPPSKIKPNLTVAKDGSGDFKTIRGALDSIPKPKKGSTQTFVLYI 302

Query: 307 KAGVYQEYVLVKKTLTHLMLIGDGPKKTIITGNKNFIDGTPTFKTATVAVTAEHFMARDI 366
           KAGVYQEYV   K++ +LM+IGDGP+KT ITGNKNF+DG  T+ TATV V  ++F+A++I
Sbjct: 303 KAGVYQEYVEFTKSMDYLMVIGDGPEKTRITGNKNFVDGVNTYHTATVVVLGDNFIAKNI 362

Query: 367 GFENTAGPQKHQAVALRVQADKAVFYNCEMHGYQDTLYVHTMRQFYRDCTVSGTIDFIFG 426
           GFEN+AG  KHQAVALRV AD AVFYNC M GYQDTLY H  RQFYR+C++SGTIDF+FG
Sbjct: 363 GFENSAGAIKHQAVALRVSADFAVFYNCAMDGYQDTLYTHAKRQFYRECSISGTIDFVFG 422

Query: 427 DAAAIFQSCTFLVRKPLPNQQCIVTAHGRKERRQPSALIIQNCSFKPHADLVPVQKQFRS 486
           DA  +FQ+C FLVRKPL NQQCIVTA GRK+RRQPS +IIQN +F  H +LVPV+KQ++S
Sbjct: 423 DAPVVFQNCKFLVRKPLENQQCIVTAQGRKQRRQPSGIIIQNGTFTAHPELVPVKKQYKS 482

Query: 487 FLGRPWKEYSRTIIMESYIGDLIQPEGWLPWAGDWGLRTCFYTEYNNYGPGSDKSKRVKW 546
           +LGRPWK++SRTIIMESYI D+I+PEGWLPW G +GL+TC+YTE+NNYGPGSDK  RVKW
Sbjct: 483 YLGRPWKQFSRTIIMESYIDDIIEPEGWLPWFGTFGLKTCWYTEFNNYGPGSDKKARVKW 542

Query: 547 RGIKNITPQHAVDFTPGRFLKGDRWIKPTGVPYVSGLTR 575
            GIK I  QHA+DFTPGRFL+GD WIKPTG+PY   LTR
Sbjct: 543 NGIKTIDRQHALDFTPGRFLRGDTWIKPTGIPYSPFLTR 580

BLAST of Cucsa.181720 vs. TrEMBL
Match: D7U9X3_VITVI (Pectinesterase OS=Vitis vinifera GN=VIT_14s0060g01950 PE=4 SV=1)

HSP 1 Score: 732.6 bits (1890), Expect = 3.6e-208
Identity = 361/575 (62.78%), Postives = 448/575 (77.91%), Query Frame = 1

Query: 3   YKDDDSQNKKRYAIIGVSSMLLVAMVVAVTVGVNLNQDETSDPATGNKSHEISSSMKAIK 62
           Y ++D   KK+ AIIG SS+ LVAMV+AV   V+     +SD     K  EISSSMKAI+
Sbjct: 5   YYNNDRGKKKKLAIIGFSSIFLVAMVIAVIASVS---SSSSDSGNAQKQ-EISSSMKAIQ 64

Query: 63  AICQPTDYKQECVASLKATGNNSSDPKELVQAGFKAAMKLIQAAANKSVALNQLEKDPRA 122
           AICQPTDYK  CV SL +   N++DPK+LVQA F +AM+ + AAA  S  L +L KDPRA
Sbjct: 65  AICQPTDYKDACVNSLTSKAGNTTDPKDLVQAAFASAMEHLSAAAKNSTLLQELNKDPRA 124

Query: 123 SKALAGCKELMDFAIDELKYSMNKLGEFDISKLDEMLIDIRIWLSATITYQETCLDGFAN 182
           S+AL  C++L+++AID+LK S N++G+FD SK+D ++ DI+IWLSA ITYQETCLDGF N
Sbjct: 125 SQALQNCEDLVNYAIDDLKKSFNQVGDFDYSKMDNIIADIKIWLSAVITYQETCLDGFEN 184

Query: 183 TTGNAAEKMKKALKTSMKLSSNGLAMVSQISSMLSELQIPGISRRRLLEIPVLGH----D 242
           TTG+A EKM++ LKTSM+LSSNGLA+V ++SS+LS LQ+  ++RR L + P        D
Sbjct: 185 TTGDAGEKMRQILKTSMELSSNGLAIVGEVSSILSNLQLANLNRRLLSDDPADPDNHIDD 244

Query: 243 DYPDWANPGMRRLLAAG-SKVKPNVVVAKDGSGQFKTIQEAIDQVPKRKNNATYVIHIKA 302
           ++P W++   R+LL A  S++KPN+ VAKDGSG FKTI EAI Q+PK  N  T++++IK 
Sbjct: 245 EFPYWSHSEGRKLLQANVSELKPNLTVAKDGSGDFKTINEAIRQLPKFSNQ-TFILYIKK 304

Query: 303 GVYQEYVLVKKTLTHLMLIGDGPKKTIITGNKNFIDGTPTFKTATVAVTAEHFMARDIGF 362
           G+Y+E V + KT T+LM++GDGP KT ITG+ NF+DGTPTFKTATVAV  + F+A+ IGF
Sbjct: 305 GIYEEQVQINKTFTNLMMVGDGPTKTKITGSLNFVDGTPTFKTATVAVLGDGFIAKGIGF 364

Query: 363 ENTAGPQKHQAVALRVQADKAVFYNCEMHGYQDTLYVHTMRQFYRDCTVSGTIDFIFGDA 422
           EN+AG  KHQAVALRVQ+D+++FYNC+M GYQDTLY HT RQFYRDCT+SGTIDFIFGDA
Sbjct: 365 ENSAGAAKHQAVALRVQSDRSIFYNCQMDGYQDTLYTHTKRQFYRDCTISGTIDFIFGDA 424

Query: 423 AAIFQSCTFLVRKPLPNQQCIVTAHGRKERRQPSALIIQNCSFKPHADLVPVQKQFRSFL 482
           A IFQ+CTF+VRKPL NQQCIVTA GRKERRQPSA+IIQN +F    +  P + + +S+L
Sbjct: 425 AVIFQNCTFVVRKPLDNQQCIVTAQGRKERRQPSAIIIQNSTFTADPEYYPYRNELKSYL 484

Query: 483 GRPWKEYSRTIIMESYIGDLIQPEGWLPWAGDWGLRTCFYTEYNNYGPGSDKSKRVKWRG 542
           GRPWKE+SRTIIMESYI DLIQP GWLPWAGD+ LRTCFYTE+ N GPG+    RVKWRG
Sbjct: 485 GRPWKEFSRTIIMESYIEDLIQPSGWLPWAGDFALRTCFYTEFRNRGPGAKTHDRVKWRG 544

Query: 543 IKNITPQHAVDFTPGRFLKGDRWIKPTGVPYVSGL 573
           IK I P HA+DF PGRFL GDRWI  TGVPY SGL
Sbjct: 545 IKTIKPSHAIDFAPGRFLSGDRWIPSTGVPYNSGL 574

BLAST of Cucsa.181720 vs. TAIR10
Match: AT5G27870.1 (AT5G27870.1 Plant invertase/pectin methylesterase inhibitor superfamily)

HSP 1 Score: 657.5 bits (1695), Expect = 7.5e-189
Identity = 311/572 (54.37%), Postives = 431/572 (75.35%), Query Frame = 1

Query: 2   GYKDDDSQNKKRYAIIGVSSMLLVAMVVAVTVGVNLNQDETSDPATGNKSHEISSSMKAI 61
           GY D+D++ KKRY II +SS+LL++MVVAVT+GV++N+ + +         EI++S+KAI
Sbjct: 4   GYDDEDAKRKKRYVIISISSVLLISMVVAVTIGVSVNKSDNAG------DEEITTSVKAI 63

Query: 62  KAICQPTDYKQECVASLKATGNNSSDPKELVQAGFKAAMKLIQAAANKSVALNQLEKDPR 121
           K +C PTDYK+ C  +L+    ++SDP ELV+  F A MK I   A KS  + +L+KDPR
Sbjct: 64  KDVCAPTDYKETCEDTLRKDAKDTSDPLELVKTAFNATMKQISDVAKKSQTMIELQKDPR 123

Query: 122 ASKALAGCKELMDFAIDELKYSMNKLGEFDISKLDEMLIDIRIWLSATITYQETCLDGFA 181
           A  AL  CKELMD+AI EL  S  +LG+F+  K+DE L+ +RIWLSATI++++TCLDGF 
Sbjct: 124 AKMALDQCKELMDYAIGELSKSFEELGKFEFHKVDEALVKLRIWLSATISHEQTCLDGFQ 183

Query: 182 NTTGNAAEKMKKALKTSMKLSSNGLAMVSQISSMLSELQIPGISRRRLLEIPVLGHDDYP 241
            T GNA E +KKALKT+++L+ NGLAMV+++S+ L ++QIP ++ RRLL        ++P
Sbjct: 184 GTQGNAGETIKKALKTAVQLTHNGLAMVTEMSNYLGQMQIPEMNSRRLLS------QEFP 243

Query: 242 DWANPGMRRLLAAG-SKVKPNVVVAKDGSGQFKTIQEAIDQVPKRKNNATYVIHIKAGVY 301
            W +   RRLL A  S+VKP++VVA+DGSGQ+KTI EA++ VPK+KN  T+V+HIK G+Y
Sbjct: 244 SWMDARARRLLNAPMSEVKPDIVVAQDGSGQYKTINEALNFVPKKKNT-TFVVHIKEGIY 303

Query: 302 QEYVLVKKTLTHLMLIGDGPKKTIITGNKNFIDGTPTFKTATVAVTAEHFMARDIGFENT 361
           +EYV V +++THL+ IGDGP KT+I+G+K++ DG  T+KTATVA+  +HF+A++I FENT
Sbjct: 304 KEYVQVNRSMTHLVFIGDGPDKTVISGSKSYKDGITTYKTATVAIVGDHFIAKNIAFENT 363

Query: 362 AGPQKHQAVALRVQADKAVFYNCEMHGYQDTLYVHTMRQFYRDCTVSGTIDFIFGDAAAI 421
           AG  KHQAVA+RV AD+++FYNC+  GYQDTLY H+ RQFYRDCT+SGTIDF+FGDAAA+
Sbjct: 364 AGAIKHQAVAIRVLADESIFYNCKFDGYQDTLYAHSHRQFYRDCTISGTIDFLFGDAAAV 423

Query: 422 FQSCTFLVRKPLPNQQCIVTAHGRKERRQPSALIIQNCSFKPHADLVPVQKQFRSFLGRP 481
           FQ+CT LVRKPL NQ C +TAHGRK+ R+ +  ++Q C+     D + V++Q +++LGRP
Sbjct: 424 FQNCTLLVRKPLLNQACPITAHGRKDPRESTGFVLQGCTIVGEPDYLAVKEQSKTYLGRP 483

Query: 482 WKEYSRTIIMESYIGDLIQPEGWLPWAGDWGLRTCFYTEYNNYGPGSDKSKRVKWRGIKN 541
           WKEYSRTIIM ++I D + PEGW PW G++GL T FY+E  N GPG+  +KRV W GIK 
Sbjct: 484 WKEYSRTIIMNTFIPDFVPPEGWQPWLGEFGLNTLFYSEVQNTGPGAAITKRVTWPGIKK 543

Query: 542 ITPQHAVDFTPGRFLKGDRWIKPTGVPYVSGL 573
           ++ +  + FTP ++++GD WI   GVPY+ GL
Sbjct: 544 LSDEEILKFTPAQYIQGDAWIPGKGVPYILGL 562

BLAST of Cucsa.181720 vs. TAIR10
Match: AT3G05610.1 (AT3G05610.1 Plant invertase/pectin methylesterase inhibitor superfamily)

HSP 1 Score: 652.5 bits (1682), Expect = 2.4e-187
Identity = 311/574 (54.18%), Postives = 428/574 (74.56%), Query Frame = 1

Query: 2   GYKDDDSQNKKRYAIIGVSSMLLVAMVVAVTVGVNLNQDETSDPATGNKSHEISSSMKAI 61
           GY DD+S+ K+RY +I +SS+LL++MVVAVTVGV+LN+ +           E+++S+KA+
Sbjct: 4   GY-DDESKRKRRYIVITISSVLLISMVVAVTVGVSLNKHDGDSKGKA----EVNASVKAV 63

Query: 62  KAICQPTDYKQECVASLKATGNNSSDPKELVQAGFKAAMKLIQAAANKSVALNQLEKDPR 121
           K +C PTDY++ C  +L   G N++DP ELV+  F   MK I  AA KS  + +L+KD R
Sbjct: 64  KDVCAPTDYRKTCEDTLIKNGKNTTDPMELVKTAFNVTMKQITDAAKKSQTIMELQKDSR 123

Query: 122 ASKALAGCKELMDFAIDELKYSMNKLGEFDISKLDEMLIDIRIWLSATITYQETCLDGFA 181
              AL  CKELMD+A+DEL  S  +LG+F+   LDE LI++RIWLSA I+++ETCL+GF 
Sbjct: 124 TRMALDQCKELMDYALDELSNSFEELGKFEFHLLDEALINLRIWLSAAISHEETCLEGFQ 183

Query: 182 NTTGNAAEKMKKALKTSMKLSSNGLAMVSQISSMLSELQIPGISRRRLLEIPVLGHDDYP 241
            T GNA E MKKALKT+++L+ NGLA++S++S+ + ++QIPG++ RRLL       + +P
Sbjct: 184 GTQGNAGETMKKALKTAIELTHNGLAIISEMSNFVGQMQIPGLNSRRLLA------EGFP 243

Query: 242 DWANPGMRRLL---AAGSKVKPNVVVAKDGSGQFKTIQEAIDQVPKRKNNATYVIHIKAG 301
            W +   R+LL   AA S VKP++VVA+DGSGQ+KTI EA+  VPK K N T+V+HIKAG
Sbjct: 244 SWVDQRGRKLLQAAAAYSDVKPDIVVAQDGSGQYKTINEALQFVPK-KRNTTFVVHIKAG 303

Query: 302 VYQEYVLVKKTLTHLMLIGDGPKKTIITGNKNFIDGTPTFKTATVAVTAEHFMARDIGFE 361
           +Y+EYV V KT++HL+ IGDGP KTII+GNKN+ DG  T++TATVA+   +F+A++IGFE
Sbjct: 304 LYKEYVQVNKTMSHLVFIGDGPDKTIISGNKNYKDGITTYRTATVAIVGNYFIAKNIGFE 363

Query: 362 NTAGPQKHQAVALRVQADKAVFYNCEMHGYQDTLYVHTMRQFYRDCTVSGTIDFIFGDAA 421
           NTAG  KHQAVA+RVQ+D+++F+NC   GYQDTLY H+ RQF+RDCT+SGTIDF+FGDAA
Sbjct: 364 NTAGAIKHQAVAVRVQSDESIFFNCRFDGYQDTLYTHSHRQFFRDCTISGTIDFLFGDAA 423

Query: 422 AIFQSCTFLVRKPLPNQQCIVTAHGRKERRQPSALIIQNCSFKPHADLVPVQKQFRSFLG 481
           A+FQ+CT LVRKPLPNQ C +TAHGRK+ R+ +  + Q C+     D + V++  +++LG
Sbjct: 424 AVFQNCTLLVRKPLPNQACPITAHGRKDPRESTGFVFQGCTIAGEPDYLAVKETSKAYLG 483

Query: 482 RPWKEYSRTIIMESYIGDLIQPEGWLPWAGDWGLRTCFYTEYNNYGPGSDKSKRVKWRGI 541
           RPWKEYSRTIIM ++I D +QP+GW PW GD+GL+T FY+E  N GPGS  + RV W GI
Sbjct: 484 RPWKEYSRTIIMNTFIPDFVQPQGWQPWLGDFGLKTLFYSEVQNTGPGSALANRVTWAGI 543

Query: 542 KNITPQHAVDFTPGRFLKGDRWIKPTGVPYVSGL 573
           K ++ +  + FTP ++++GD WI   GVPY +GL
Sbjct: 544 KTLSEEDILKFTPAQYIQGDDWIPGKGVPYTTGL 565

BLAST of Cucsa.181720 vs. TAIR10
Match: AT5G49180.1 (AT5G49180.1 Plant invertase/pectin methylesterase inhibitor superfamily)

HSP 1 Score: 539.7 bits (1389), Expect = 2.3e-153
Identity = 274/575 (47.65%), Postives = 381/575 (66.26%), Query Frame = 1

Query: 6   DDSQNKKRYAIIGVSSMLLVAMVVAV--TVGVNLNQDETSDPATGNKSHEISSSMKAIKA 65
           D    KK+  I GV + LLV MVVAV  T   N +  E   P       +I ++  A++A
Sbjct: 4   DGELKKKKCIIAGVITALLVLMVVAVGITTSRNTSHSEKIVPV------QIKTATTAVEA 63

Query: 66  ICQPTDYKQECVASLKATGNNSSDPKELVQAGFKAAMKLIQAAANK-SVALNQLEKDPRA 125
           +C PTDYK+ CV SL     +S+ P +L++ GF   ++ I+ +  K SV L     + + 
Sbjct: 64  VCAPTDYKETCVNSLMKASPDSTQPLDLIKLGFNVTIRSIEDSIKKASVELTAKAANDKD 123

Query: 126 SK-ALAGCKELMDFAIDELKYSMNKLGEFDISKLDEMLIDIRIWLSATITYQETCLDGFA 185
           +K AL  C++LM+ A D+LK  ++    F I ++++ + D+R+WLS +I YQ+TC+D F 
Sbjct: 124 TKGALELCEKLMNDATDDLKKCLDNFDGFSIPQIEDFVEDLRVWLSGSIAYQQTCMDTFE 183

Query: 186 NTTGNAAEKMKKALKTSMKLSSNGLAMVSQISSMLSELQIPGIS------RRRLLEIPVL 245
            T    ++ M+K  KTS +L+SNGLAM++ IS++L E  + G++       R+LL     
Sbjct: 184 ETNSKLSQDMQKIFKTSRELTSNGLAMITNISNLLGEFNVTGVTGDLGKYARKLLS---- 243

Query: 246 GHDDYPDWANPGMRRLLAAGSKVKPNVVVAKDGSGQFKTIQEAIDQVPKRKNNATYVIHI 305
             D  P W  P  RRL+A    VK NVVVA DGSGQ+KTI EA++ VPK  N   +VI+I
Sbjct: 244 AEDGIPSWVGPNTRRLMATKGGVKANVVVAHDGSGQYKTINEALNAVPKA-NQKPFVIYI 303

Query: 306 KAGVYQEYVLVKKTLTHLMLIGDGPKKTIITGNKNFIDG-TPTFKTATVAVTAEHFMARD 365
           K GVY E V V K +TH+  IGDGP KT ITG+ N+  G   T+ TATVA+  ++F A++
Sbjct: 304 KQGVYNEKVDVTKKMTHVTFIGDGPTKTKITGSLNYYIGKVKTYLTATVAINGDNFTAKN 363

Query: 366 IGFENTAGPQKHQAVALRVQADKAVFYNCEMHGYQDTLYVHTMRQFYRDCTVSGTIDFIF 425
           IGFENTAGP+ HQAVALRV AD AVFYNC++ GYQDTLYVH+ RQF+RDCTVSGT+DFIF
Sbjct: 364 IGFENTAGPEGHQAVALRVSADLAVFYNCQIDGYQDTLYVHSHRQFFRDCTVSGTVDFIF 423

Query: 426 GDAAAIFQSCTFLVRKPLPNQQCIVTAHGRKERRQPSALIIQNCSFKPHADLVPVQKQFR 485
           GD   + Q+C  +VRKP+ +Q C++TA GR ++R+ + L++QNC        +PV+   +
Sbjct: 424 GDGIVVLQNCNIVVRKPMKSQSCMITAQGRSDKRESTGLVLQNCHITGEPAYIPVKSINK 483

Query: 486 SFLGRPWKEYSRTIIMESYIGDLIQPEGWLPWAGDWGLRTCFYTEYNNYGPGSDKSKRVK 545
           ++LGRPWKE+SRTIIM + I D+I P GWLPW GD+ L T +Y EY N GPGS++++RVK
Sbjct: 484 AYLGRPWKEFSRTIIMGTTIDDVIDPAGWLPWNGDFALNTLYYAEYENNGPGSNQAQRVK 543

Query: 546 WRGIKNITPQHAVDFTPGRFLKGDRWIKPTGVPYV 570
           W GIK ++P+ A+ FTP RFL+G+ WI P  VPY+
Sbjct: 544 WPGIKKLSPKQALRFTPARFLRGNLWIPPNRVPYM 567

BLAST of Cucsa.181720 vs. TAIR10
Match: AT3G06830.1 (AT3G06830.1 Plant invertase/pectin methylesterase inhibitor superfamily)

HSP 1 Score: 510.4 bits (1313), Expect = 1.5e-144
Identity = 264/577 (45.75%), Postives = 370/577 (64.12%), Query Frame = 1

Query: 7   DSQNKKRYAIIGVSSMLLVAMVVAVTVGVNLNQDETSDPATGNKSHEISSSMKAIKAICQ 66
           D   KK++ + G  S  LV MVV+V V        TS  +  +  + I  + KA++A+C 
Sbjct: 4   DGDKKKKFIVAGSVSGFLVIMVVSVAV-------VTSKHSPKDDENHIRKTTKAVQAVCA 63

Query: 67  PTDYKQECVASLKATGNNSSDPKELVQAGFKAAMKLIQAAANKSVA--LNQLEKDPRASK 126
           PTD+K  CV SL     +S DP +L++ GFK  +K I  +  K+      + +K+P A  
Sbjct: 64  PTDFKDTCVNSLMGASPDSDDPVDLIKLGFKVTIKSINESLEKASGDIKAKADKNPEAKG 123

Query: 127 ALAGCKELMDFAIDELKYSMNKLGEFDISKLDEMLIDIRIWLSATITYQETCLDGFANTT 186
           A   C++LM  AID+LK  M+    F + +++  + D+R+WLS +I +Q+TC+D F    
Sbjct: 124 AFELCEKLMIDAIDDLKKCMDH--GFSVDQIEVFVEDLRVWLSGSIAFQQTCMDSFGEIK 183

Query: 187 GNAAEKMKKALKTSMKLSSNGLAMVSQISSMLSELQIPGIS------RRRLLEIPVLGHD 246
            N  + M K  KTS +LSSN LAMV++IS+++    + G++       R+LL       D
Sbjct: 184 SNLMQDMLKIFKTSRELSSNSLAMVTRISTLIPNSNLTGLTGALAKYARKLLST----ED 243

Query: 247 DYPDWANPGMRRLLAAGS----KVKPNVVVAKDGSGQFKTIQEAIDQVPKRKNNATYVIH 306
             P W  P  RRL+AA       VK N VVA+DG+GQFKTI +A++ VPK  N   ++IH
Sbjct: 244 SIPTWVGPEARRLMAAQGGGPGPVKANAVVAQDGTGQFKTITDALNAVPKG-NKVPFIIH 303

Query: 307 IKAGVYQEYVLVKKTLTHLMLIGDGPKKTIITGNKNF-IDGTPTFKTATVAVTAEHFMAR 366
           IK G+Y+E V V K + H+  IGDGP KT+ITG+ NF I    TF TAT+ +  +HF A+
Sbjct: 304 IKEGIYKEKVTVTKKMPHVTFIGDGPNKTLITGSLNFGIGKVKTFLTATITIEGDHFTAK 363

Query: 367 DIGFENTAGPQKHQAVALRVQADKAVFYNCEMHGYQDTLYVHTMRQFYRDCTVSGTIDFI 426
           +IG ENTAGP+  QAVALRV AD AVF++C++ G+QDTLYVH+ RQFYRDCTVSGT+DFI
Sbjct: 364 NIGIENTAGPEGGQAVALRVSADYAVFHSCQIDGHQDTLYVHSHRQFYRDCTVSGTVDFI 423

Query: 427 FGDAAAIFQSCTFLVRKPLPNQQCIVTAHGRKERRQPSALIIQNCSFKPHADLVPVQKQF 486
           FGDA  I Q+C  +VRKP   Q C+VTA GR   R+ + L++  C        +P++   
Sbjct: 424 FGDAKCILQNCKIVVRKPNKGQTCMVTAQGRSNVRESTGLVLHGCHITGDPAYIPMKSVN 483

Query: 487 RSFLGRPWKEYSRTIIMESYIGDLIQPEGWLPWAGDWGLRTCFYTEYNNYGPGSDKSKRV 546
           +++LGRPWKE+SRTIIM++ I D+I P GWLPW+GD+ L+T +Y E+ N GPGS++++RV
Sbjct: 484 KAYLGRPWKEFSRTIIMKTTIDDVIDPAGWLPWSGDFALKTLYYAEHMNTGPGSNQAQRV 543

Query: 547 KWRGIKNITPQHAVDFTPGRFLKGDRWIKPTGVPYVS 571
           KW GIK +TPQ A+ +T  RFL+GD WI  T VPY +
Sbjct: 544 KWPGIKKLTPQDALLYTGDRFLRGDTWIPQTQVPYTA 566

BLAST of Cucsa.181720 vs. TAIR10
Match: AT5G04960.1 (AT5G04960.1 Plant invertase/pectin methylesterase inhibitor superfamily)

HSP 1 Score: 430.3 bits (1105), Expect = 1.9e-120
Identity = 249/571 (43.61%), Postives = 346/571 (60.60%), Query Frame = 1

Query: 9   QNKKRYAIIGVSSMLLVAMVVAVTVGVNLNQDETSDPATGNKSHEISSSMKAIKAICQPT 68
           + KKR AII +SS++LV +VV   VG    +D +  P T N    IS S   +KA+C  T
Sbjct: 20  KTKKRIAIIAISSIVLVCIVVGAVVGTTA-RDNSKKPPTENNGEPISVS---VKALCDVT 79

Query: 69  DYKQECVASLKATGNNS-SDPKELVQAGFKAAMKLIQAAANKSVA--LNQLEKDPRASKA 128
            +K++C  +L +  N S S P+EL    FK A+K+     +K +    N    D   S A
Sbjct: 80  LHKEKCFETLGSAPNASRSSPEEL----FKYAVKVTITELSKVLDGFSNGEHMDNATSAA 139

Query: 129 LAGCKELMDFAIDELKYSM-NKLGEFDISKLDEMLIDIRIWLSATITYQETCLDGFANTT 188
           +  C EL+  A+D+L  +M + L  FD         D+R WLS+  TYQETC+D      
Sbjct: 140 MGACVELIGLAVDQLNETMTSSLKNFD---------DLRTWLSSVGTYQETCMDALVEAN 199

Query: 189 GNAAEKM-KKALKTSMKLSSNGLAMVSQISSMLSELQIPGISRRRLLEIPVLGHDDYPDW 248
             +     +  LK S +++SN LA+++ +  +   ++     RRRLLE    G+      
Sbjct: 200 KPSLTTFGENHLKNSTEMTSNALAIITWLGKIADTVKF---RRRRLLET---GNAKVVVA 259

Query: 249 ANPGM--RRLLAAGS-KVKPNVVVAKDGSGQFKTIQEAIDQVPKRKNNATYVIHIKAGVY 308
             P M  RRLL +G  K K  +VVAKDGSG+++TI EA+ +V + KN    +I++K GVY
Sbjct: 260 DLPMMEGRRLLESGDLKKKATIVVAKDGSGKYRTIGEALAEV-EEKNEKPTIIYVKKGVY 319

Query: 309 QEYVLVKKTLTHLMLIGDGPKKTIITGNKNFIDGTPTFKTATVAVTAEHFMARDIGFENT 368
            E V V+KT  +++++GDG  KTI++   NFIDGTPTF+TAT AV  + FMARD+GF NT
Sbjct: 320 LENVRVEKTKWNVVMVGDGQSKTIVSAGLNFIDGTPTFETATFAVFGKGFMARDMGFINT 379

Query: 369 AGPQKHQAVALRVQADKAVFYNCEMHGYQDTLYVHTMRQFYRDCTVSGTIDFIFGDAAAI 428
           AGP KHQAVAL V AD +VFY C M  +QDT+Y H  RQFYRDC + GT+DFIFG+AA +
Sbjct: 380 AGPAKHQAVALMVSADLSVFYKCTMDAFQDTMYAHAQRQFYRDCVILGTVDFIFGNAAVV 439

Query: 429 FQSCTFLVRKPLPNQQCIVTAHGRKERRQPSALIIQNCSFKPHADLVPVQKQFRSFLGRP 488
           FQ C  L R+P+  QQ  +TA GRK+  Q + + I NC+ KP  +L  +Q    +FLGRP
Sbjct: 440 FQKCEILPRRPMKGQQNTITAQGRKDPNQNTGISIHNCTIKPLDNLTDIQ----TFLGRP 499

Query: 489 WKEYSRTIIMESYIGDLIQPEGWLPWAGDWGLRTCFYTEYNNYGPGSDKSKRVKWRGIK- 548
           WK++S T+IM+S++   I P+GWLPW GD    T FY EY N GPG+    RVKW+G+K 
Sbjct: 500 WKDFSTTVIMKSFMDKFINPKGWLPWTGDTAPDTIFYAEYLNSGPGASTKNRVKWQGLKT 559

Query: 549 NITPQHAVDFTPGRFLKGDRWIKPTGVPYVS 571
           ++T + A  FT   F+ G+ W+  T VP+ S
Sbjct: 560 SLTKKEANKFTVKPFIDGNNWLPATKVPFNS 562

BLAST of Cucsa.181720 vs. NCBI nr
Match: gi|449435986|ref|XP_004135775.1| (PREDICTED: putative pectinesterase/pectinesterase inhibitor 28 [Cucumis sativus])

HSP 1 Score: 1164.1 bits (3010), Expect = 0.0e+00
Identity = 576/576 (100.00%), Postives = 576/576 (100.00%), Query Frame = 1

Query: 1   MGYKDDDSQNKKRYAIIGVSSMLLVAMVVAVTVGVNLNQDETSDPATGNKSHEISSSMKA 60
           MGYKDDDSQNKKRYAIIGVSSMLLVAMVVAVTVGVNLNQDETSDPATGNKSHEISSSMKA
Sbjct: 1   MGYKDDDSQNKKRYAIIGVSSMLLVAMVVAVTVGVNLNQDETSDPATGNKSHEISSSMKA 60

Query: 61  IKAICQPTDYKQECVASLKATGNNSSDPKELVQAGFKAAMKLIQAAANKSVALNQLEKDP 120
           IKAICQPTDYKQECVASLKATGNNSSDPKELVQAGFKAAMKLIQAAANKSVALNQLEKDP
Sbjct: 61  IKAICQPTDYKQECVASLKATGNNSSDPKELVQAGFKAAMKLIQAAANKSVALNQLEKDP 120

Query: 121 RASKALAGCKELMDFAIDELKYSMNKLGEFDISKLDEMLIDIRIWLSATITYQETCLDGF 180
           RASKALAGCKELMDFAIDELKYSMNKLGEFDISKLDEMLIDIRIWLSATITYQETCLDGF
Sbjct: 121 RASKALAGCKELMDFAIDELKYSMNKLGEFDISKLDEMLIDIRIWLSATITYQETCLDGF 180

Query: 181 ANTTGNAAEKMKKALKTSMKLSSNGLAMVSQISSMLSELQIPGISRRRLLEIPVLGHDDY 240
           ANTTGNAAEKMKKALKTSMKLSSNGLAMVSQISSMLSELQIPGISRRRLLEIPVLGHDDY
Sbjct: 181 ANTTGNAAEKMKKALKTSMKLSSNGLAMVSQISSMLSELQIPGISRRRLLEIPVLGHDDY 240

Query: 241 PDWANPGMRRLLAAGSKVKPNVVVAKDGSGQFKTIQEAIDQVPKRKNNATYVIHIKAGVY 300
           PDWANPGMRRLLAAGSKVKPNVVVAKDGSGQFKTIQEAIDQVPKRKNNATYVIHIKAGVY
Sbjct: 241 PDWANPGMRRLLAAGSKVKPNVVVAKDGSGQFKTIQEAIDQVPKRKNNATYVIHIKAGVY 300

Query: 301 QEYVLVKKTLTHLMLIGDGPKKTIITGNKNFIDGTPTFKTATVAVTAEHFMARDIGFENT 360
           QEYVLVKKTLTHLMLIGDGPKKTIITGNKNFIDGTPTFKTATVAVTAEHFMARDIGFENT
Sbjct: 301 QEYVLVKKTLTHLMLIGDGPKKTIITGNKNFIDGTPTFKTATVAVTAEHFMARDIGFENT 360

Query: 361 AGPQKHQAVALRVQADKAVFYNCEMHGYQDTLYVHTMRQFYRDCTVSGTIDFIFGDAAAI 420
           AGPQKHQAVALRVQADKAVFYNCEMHGYQDTLYVHTMRQFYRDCTVSGTIDFIFGDAAAI
Sbjct: 361 AGPQKHQAVALRVQADKAVFYNCEMHGYQDTLYVHTMRQFYRDCTVSGTIDFIFGDAAAI 420

Query: 421 FQSCTFLVRKPLPNQQCIVTAHGRKERRQPSALIIQNCSFKPHADLVPVQKQFRSFLGRP 480
           FQSCTFLVRKPLPNQQCIVTAHGRKERRQPSALIIQNCSFKPHADLVPVQKQFRSFLGRP
Sbjct: 421 FQSCTFLVRKPLPNQQCIVTAHGRKERRQPSALIIQNCSFKPHADLVPVQKQFRSFLGRP 480

Query: 481 WKEYSRTIIMESYIGDLIQPEGWLPWAGDWGLRTCFYTEYNNYGPGSDKSKRVKWRGIKN 540
           WKEYSRTIIMESYIGDLIQPEGWLPWAGDWGLRTCFYTEYNNYGPGSDKSKRVKWRGIKN
Sbjct: 481 WKEYSRTIIMESYIGDLIQPEGWLPWAGDWGLRTCFYTEYNNYGPGSDKSKRVKWRGIKN 540

Query: 541 ITPQHAVDFTPGRFLKGDRWIKPTGVPYVSGLTRTG 577
           ITPQHAVDFTPGRFLKGDRWIKPTGVPYVSGLTRTG
Sbjct: 541 ITPQHAVDFTPGRFLKGDRWIKPTGVPYVSGLTRTG 576

BLAST of Cucsa.181720 vs. NCBI nr
Match: gi|659099441|ref|XP_008450602.1| (PREDICTED: probable pectinesterase/pectinesterase inhibitor 21 [Cucumis melo])

HSP 1 Score: 1111.7 bits (2874), Expect = 0.0e+00
Identity = 544/576 (94.44%), Postives = 560/576 (97.22%), Query Frame = 1

Query: 1   MGYKDDDSQNKKRYAIIGVSSMLLVAMVVAVTVGVNLNQDETSDPATGNKSHEISSSMKA 60
           MGYKDDDSQNKKRYAIIGVSSMLLVAMVVAVTVGVNLNQDETSDPATGNKSHEISSSMKA
Sbjct: 1   MGYKDDDSQNKKRYAIIGVSSMLLVAMVVAVTVGVNLNQDETSDPATGNKSHEISSSMKA 60

Query: 61  IKAICQPTDYKQECVASLKATGNNSSDPKELVQAGFKAAMKLIQAAANKSVALNQLEKDP 120
           IKAICQPTDYKQEC+ASLKATGNNSSDP+ELVQAGFKAAMKLIQAAANKSV L QLEKDP
Sbjct: 61  IKAICQPTDYKQECIASLKATGNNSSDPQELVQAGFKAAMKLIQAAANKSVDLKQLEKDP 120

Query: 121 RASKALAGCKELMDFAIDELKYSMNKLGEFDISKLDEMLIDIRIWLSATITYQETCLDGF 180
           R SKALAGCKELMDFAIDELKYSMNKLGEFDI+KLDEMLIDIRIWLSATITYQETCLDGF
Sbjct: 121 RTSKALAGCKELMDFAIDELKYSMNKLGEFDITKLDEMLIDIRIWLSATITYQETCLDGF 180

Query: 181 ANTTGNAAEKMKKALKTSMKLSSNGLAMVSQISSMLSELQIPGISRRRLLEIPVLGHDDY 240
            NTTGNAAEKMKKALKTSMKLSSNGLAMVSQ SSML+ELQIPGISRRRLLEIPVLGHDDY
Sbjct: 181 QNTTGNAAEKMKKALKTSMKLSSNGLAMVSQFSSMLTELQIPGISRRRLLEIPVLGHDDY 240

Query: 241 PDWANPGMRRLLAAGSKVKPNVVVAKDGSGQFKTIQEAIDQVPKRKNNATYVIHIKAGVY 300
           PDWANPGMRRLLAAGS+VKPNVVVAKDGSGQF+TIQ A+DQVPKRKNNATYVIHIKAGVY
Sbjct: 241 PDWANPGMRRLLAAGSRVKPNVVVAKDGSGQFRTIQAALDQVPKRKNNATYVIHIKAGVY 300

Query: 301 QEYVLVKKTLTHLMLIGDGPKKTIITGNKNFIDGTPTFKTATVAVTAEHFMARDIGFENT 360
           QEY+ +KKT+THLMLIGDGPKKTIITGNKNF+DGTPTFKTATVAVTAEHFMARDIGFENT
Sbjct: 301 QEYLQIKKTVTHLMLIGDGPKKTIITGNKNFVDGTPTFKTATVAVTAEHFMARDIGFENT 360

Query: 361 AGPQKHQAVALRVQADKAVFYNCEMHGYQDTLYVHTMRQFYRDCTVSGTIDFIFGDAAAI 420
           AGPQKHQAVALRVQADKAVFYNCEMHGYQDTLYVHTMRQFYRDCTVSGTIDFIFGDAAAI
Sbjct: 361 AGPQKHQAVALRVQADKAVFYNCEMHGYQDTLYVHTMRQFYRDCTVSGTIDFIFGDAAAI 420

Query: 421 FQSCTFLVRKPLPNQQCIVTAHGRKERRQPSALIIQNCSFKPHADLVPVQKQFRSFLGRP 480
           FQ CTFLVRKPLPNQQCIVTAHGRKERRQPSALIIQNCS K   DL PVQKQFRSFLGRP
Sbjct: 421 FQGCTFLVRKPLPNQQCIVTAHGRKERRQPSALIIQNCSIKADKDLAPVQKQFRSFLGRP 480

Query: 481 WKEYSRTIIMESYIGDLIQPEGWLPWAGDWGLRTCFYTEYNNYGPGSDKSKRVKWRGIKN 540
           WKEYSRTIIMESYIGDLIQPEGWLPWAGDWGL+TCFYTEYNNYGPGS+KSKRVKWRGIK 
Sbjct: 481 WKEYSRTIIMESYIGDLIQPEGWLPWAGDWGLKTCFYTEYNNYGPGSNKSKRVKWRGIKT 540

Query: 541 ITPQHAVDFTPGRFLKGDRWIKPTGVPYVSGLTRTG 577
           ITPQHA+DFT GRF+ GDRWIKPTGVPYVSG+TRTG
Sbjct: 541 ITPQHALDFTAGRFVMGDRWIKPTGVPYVSGMTRTG 576

BLAST of Cucsa.181720 vs. NCBI nr
Match: gi|703091827|ref|XP_010094457.1| (Putative pectinesterase/pectinesterase inhibitor 28 [Morus notabilis])

HSP 1 Score: 788.1 bits (2034), Expect = 1.0e-224
Identity = 383/576 (66.49%), Postives = 471/576 (81.77%), Query Frame = 1

Query: 6   DDSQNKKRYAIIGVSSMLLVAMVVAVTVGVNLNQDETSDPATGNK--SHEISSSMKAIKA 65
           DD + +KRYA+IG+SS+LLVAMV+AVTVGV+LN  ++S PA+ NK  SHE+S+SMKAIK+
Sbjct: 5   DDGEKRKRYAVIGISSVLLVAMVIAVTVGVSLNNGQSS-PASSNKDGSHEVSASMKAIKS 64

Query: 66  ICQPTDYKQECVASLKATGNNSSDPKELVQAGFKAAMKLIQAAANKSVALNQLEKDPRAS 125
           ICQPTDYK+EC+ SL +   N++DPK+L+QAGFK AMK +  AA KSV L  LEKDPRAS
Sbjct: 65  ICQPTDYKEECINSLSSESGNTTDPKKLIQAGFKVAMKYLSEAAKKSVVLQDLEKDPRAS 124

Query: 126 KALAGCKELMDFAIDELKYSMNKLGEFDISKLDEMLIDIRIWLSATITYQETCLDGFANT 185
           KAL  CK+LM+ +ID+LK+S++KLGEFDISK D+ML+D++IWLSAT+TYQETCLDGF NT
Sbjct: 125 KALGHCKQLMENSIDDLKHSVDKLGEFDISKFDQMLMDLKIWLSATVTYQETCLDGFQNT 184

Query: 186 TGNAAEKMKKALKTSMKLSSNGLAMVSQISSMLSELQIPGISRRRLL-----EIPVLGHD 245
           TG AAEKMKKALKTSM+LSSNGL MVS+ISS+ S+LQ+  + RR        E+PVLGH 
Sbjct: 185 TGEAAEKMKKALKTSMRLSSNGLTMVSEISSVFSDLQLSSMGRRLFSDEEESELPVLGHS 244

Query: 246 DYPDWANPGMRRLLAAG-SKVKPNVVVAKDGSGQFKTIQEAIDQVPKRKNNATYVIHIKA 305
           D  +W   G+RRL+A   +KVKPN+VVAKDGSG++ TI EA+  +P+R  N T+VI+IK 
Sbjct: 245 DEAEWFGGGVRRLMAVHPTKVKPNIVVAKDGSGKYTTINEALKDIPRRSEN-TFVIYIKE 304

Query: 306 GVYQEYVLVKKTLTHLMLIGDGPKKTIITGNKNFIDGTPTFKTATVAVTAEHFMARDIGF 365
           GVY+EYV +++ +T+L++IGDG  KT ITG+KNFIDG PT  TATV V  +HF ARDIGF
Sbjct: 305 GVYKEYVTIERNMTNLLIIGDGANKTRITGDKNFIDGFPTSLTATVVVLGDHFRARDIGF 364

Query: 366 ENTAGPQKHQAVALRVQADKAVFYNCEMHGYQDTLYVHTMRQFYRDCTVSGTIDFIFGDA 425
           ENTAG  KHQAVALRV AD+A+FY C M GYQDTLY H  RQFYRDC VSGTIDF+FGDA
Sbjct: 365 ENTAGAHKHQAVALRVNADEAIFYRCAMDGYQDTLYTHAKRQFYRDCNVSGTIDFVFGDA 424

Query: 426 AAIFQSCTFLVRKPLPNQQCIVTAHGRKERRQPSALIIQNCSFKPHADLVPVQKQFRSFL 485
           AA+FQ+C F+VR+P+ NQQCIVTA GRKERRQPSA+IIQN +F    D  PV+ +  ++L
Sbjct: 425 AAVFQNCRFIVRRPMDNQQCIVTAQGRKERRQPSAIIIQNGTFVADPDYYPVRFELPAYL 484

Query: 486 GRPWKEYSRTIIMESYIGDLIQPEGWLPWAGDWGLRTCFYTEYNNYGPGS-DKSKRVKWR 545
           GRPWKE+SRTIIMES+I DLIQP GWLPWAGD+GLRTCFYTE+ N GPG+ + S+R KW+
Sbjct: 485 GRPWKEFSRTIIMESFIDDLIQPRGWLPWAGDFGLRTCFYTEFGNRGPGAVEPSRRAKWK 544

Query: 546 GIKNITPQHAVDFTPGRFLKGDRWIKPTGVPYVSGL 573
           G+KNIT +HA DFTPG F KGD WIK TG+PYVSG+
Sbjct: 545 GVKNITAKHARDFTPGSFFKGDWWIKRTGIPYVSGM 578

BLAST of Cucsa.181720 vs. NCBI nr
Match: gi|255542792|ref|XP_002512459.1| (PREDICTED: probable pectinesterase/pectinesterase inhibitor 21 [Ricinus communis])

HSP 1 Score: 751.5 bits (1939), Expect = 1.1e-213
Identity = 371/578 (64.19%), Postives = 459/578 (79.41%), Query Frame = 1

Query: 6   DDSQNKKRYAIIGVSSMLLVAMVVAVTVGVNLNQD-ETSDPATG--NKSHEISSSMKAIK 65
           +DS+ KKR AIIGVSS LLVAMVVAVTV +  N D E+ D   G  +KS E+S+SMKAIK
Sbjct: 5   EDSKRKKRLAIIGVSSFLLVAMVVAVTVSIGFNNDGESEDDINGKNHKSKEVSASMKAIK 64

Query: 66  AICQPTDYKQECVASLKATGNNSSDPKELVQAGFKAAMKLIQAAANKSVALNQLEKDPRA 125
            +CQPT YKQ C  SL  +  N++DPKEL++  FK A K I +A+ KS+ L +LEKDPR 
Sbjct: 65  TLCQPTYYKQTCERSLAKSAGNTTDPKELIKIAFKLAEKQIDSASKKSLTLLELEKDPRT 124

Query: 126 SKALAGCKELMDFAIDELKYSMNKLGEFDISKLDEMLIDIRIWLSATITYQETCLDGFAN 185
             AL  CKELM  +I+EL+ S+ K+ +FD S+LDE++ DI+ WLSA ITY+ETCLD F N
Sbjct: 125 RGALNSCKELMTMSINELRSSLEKVADFDFSQLDELMADIKTWLSAAITYEETCLDAFEN 184

Query: 186 TTGNAAEKMKKALKTSMKLSSNGLAMVSQISSMLSELQIPGISRRRLLE-IPVLGHDD-- 245
           TT NA EKMKKALKT+M++SSNGL +VS ISS+L++LQIPG+SRR L + IPV GH D  
Sbjct: 185 TTTNAGEKMKKALKTAMEMSSNGLDIVSGISSVLTDLQIPGVSRRLLQDDIPVAGHGDIS 244

Query: 246 --YPDWANPGMRRLLAAG-SKVKPNVVVAKDGSGQFKTIQEAIDQVPKRKNNATYVIHIK 305
             +P W +PG RRLL+A  S +KP++VVAKDGSG +KTI EA+ Q+PK K+N T+V++IK
Sbjct: 245 QAFPAWIDPGTRRLLSAPPSNIKPDLVVAKDGSGDYKTILEALPQIPK-KSNETFVLYIK 304

Query: 306 AGVYQEYVLVKKTLTHLMLIGDGPKKTIITGNKNFIDGTPTFKTATVAVTAEHFMARDIG 365
            G+Y+EYV   +++T+L++IGDGP KT ITG+KNF+DG  T++TATVAV  ++F+AR+IG
Sbjct: 305 EGIYEEYVEFNRSMTNLVVIGDGPDKTRITGSKNFVDGINTYRTATVAVIGDNFVARNIG 364

Query: 366 FENTAGPQKHQAVALRVQADKAVFYNCEMHGYQDTLYVHTMRQFYRDCTVSGTIDFIFGD 425
           FEN+AG  KHQAVALRV +D AVFYNC M GYQDTLY H  RQFYRDCTVSGTIDF+FGD
Sbjct: 365 FENSAGAIKHQAVALRVSSDYAVFYNCSMDGYQDTLYTHAKRQFYRDCTVSGTIDFVFGD 424

Query: 426 AAAIFQSCTFLVRKPLPNQQCIVTAHGRKERRQPSALIIQNCSFKPHADLVPVQKQFRSF 485
           A  +FQ+CTFLVRKPL NQQCIVTA GRK RRQPSA+IIQN +   H +L PV+ Q++S+
Sbjct: 425 APVVFQNCTFLVRKPLENQQCIVTAQGRKARRQPSAIIIQNSTITAHPELEPVKDQYKSY 484

Query: 486 LGRPWKEYSRTIIMESYIGDLIQPEGWLPWAGDWGLRTCFYTEYNNYGPGSDKSKRVKWR 545
           LGRPWKE+SRTIIME++I DLIQPEGW PW G +GL+TC+Y EYNNYGPGSD   RVKW 
Sbjct: 485 LGRPWKEFSRTIIMETFIDDLIQPEGWSPWFGSFGLKTCWYGEYNNYGPGSDMKNRVKWN 544

Query: 546 GIKNITPQHAVDFTPGRFLKGDRWIKPTGVPYVSGLTR 575
           GIK ++ QHA+DFTPGRFL+GD WIKPTGVPY   LTR
Sbjct: 545 GIKPVSRQHAIDFTPGRFLRGDSWIKPTGVPYAPYLTR 581

BLAST of Cucsa.181720 vs. NCBI nr
Match: gi|802760279|ref|XP_012089439.1| (PREDICTED: probable pectinesterase/pectinesterase inhibitor 21 [Jatropha curcas])

HSP 1 Score: 751.1 bits (1938), Expect = 1.4e-213
Identity = 366/579 (63.21%), Postives = 457/579 (78.93%), Query Frame = 1

Query: 7   DSQNKKRYAIIGVSSMLLVAMVVAVTVGVNLNQDETSDPATGNKSH----EISSSMKAIK 66
           D  N KR AIIG+SS+LLVAMVVAVT+GV  +++  S+  TG KSH    EIS+SMKAIK
Sbjct: 3   DDANNKRLAIIGISSILLVAMVVAVTIGVGFSENGESEDITG-KSHKSTGEISASMKAIK 62

Query: 67  AICQPTDYKQECVASLKATGNNSSDPKELVQAGFKAAMKLIQAAANKSVALNQLEKDPRA 126
           +ICQP DYK+ C  SL+ +  N++DPKEL++A FK A K I+ AA KS  L +LEKDPR 
Sbjct: 63  SICQPADYKKTCEKSLEKSAGNTTDPKELIKAAFKIAQKKIREAAKKSATLEELEKDPRT 122

Query: 127 SKALAGCKELMDFAIDELKYSMNKLGEFDISKLDEMLIDIRIWLSATITYQETCLDGFAN 186
             AL  CKELMD +I EL+ S +K+ +FDI+ LD+ + D+R WLSA ITY+ TCLDGF N
Sbjct: 123 RDALQSCKELMDMSISELQNSFDKVADFDITMLDDFMADLRTWLSAVITYEGTCLDGFEN 182

Query: 187 TTGNAAEKMKKALKTSMKLSSNGLAMVSQISSMLSELQIPGISRRRLLEIPVLGHDD--- 246
           TT +A EKMKKA+K ++++S+NGL ++  +SS L++LQI G++RR L E  VLGHDD   
Sbjct: 183 TTSDAGEKMKKAMKAAIEMSTNGLDIIDGLSSALADLQITGVNRRLLQEDEVLGHDDVPS 242

Query: 247 -YPDWANPGMRRLLAAG-SKVKPNVVVAKDGSGQFKTIQEAIDQVPKRKNNAT--YVIHI 306
            +P W + G RRLLAA  SK+KPN+ VAKDGSG FKTI+ A+D +PK K  +T  +V++I
Sbjct: 243 KFPSWVDAGTRRLLAAPPSKIKPNLTVAKDGSGDFKTIRGALDSIPKPKKGSTQTFVLYI 302

Query: 307 KAGVYQEYVLVKKTLTHLMLIGDGPKKTIITGNKNFIDGTPTFKTATVAVTAEHFMARDI 366
           KAGVYQEYV   K++ +LM+IGDGP+KT ITGNKNF+DG  T+ TATV V  ++F+A++I
Sbjct: 303 KAGVYQEYVEFTKSMDYLMVIGDGPEKTRITGNKNFVDGVNTYHTATVVVLGDNFIAKNI 362

Query: 367 GFENTAGPQKHQAVALRVQADKAVFYNCEMHGYQDTLYVHTMRQFYRDCTVSGTIDFIFG 426
           GFEN+AG  KHQAVALRV AD AVFYNC M GYQDTLY H  RQFYR+C++SGTIDF+FG
Sbjct: 363 GFENSAGAIKHQAVALRVSADFAVFYNCAMDGYQDTLYTHAKRQFYRECSISGTIDFVFG 422

Query: 427 DAAAIFQSCTFLVRKPLPNQQCIVTAHGRKERRQPSALIIQNCSFKPHADLVPVQKQFRS 486
           DA  +FQ+C FLVRKPL NQQCIVTA GRK+RRQPS +IIQN +F  H +LVPV+KQ++S
Sbjct: 423 DAPVVFQNCKFLVRKPLENQQCIVTAQGRKQRRQPSGIIIQNGTFTAHPELVPVKKQYKS 482

Query: 487 FLGRPWKEYSRTIIMESYIGDLIQPEGWLPWAGDWGLRTCFYTEYNNYGPGSDKSKRVKW 546
           +LGRPWK++SRTIIMESYI D+I+PEGWLPW G +GL+TC+YTE+NNYGPGSDK  RVKW
Sbjct: 483 YLGRPWKQFSRTIIMESYIDDIIEPEGWLPWFGTFGLKTCWYTEFNNYGPGSDKKARVKW 542

Query: 547 RGIKNITPQHAVDFTPGRFLKGDRWIKPTGVPYVSGLTR 575
            GIK I  QHA+DFTPGRFL+GD WIKPTG+PY   LTR
Sbjct: 543 NGIKTIDRQHALDFTPGRFLRGDTWIKPTGIPYSPFLTR 580